LOCUS       DAYYQN010000001        73618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYYQN010000001 DAYYQN010000000
VERSION     DAYYQN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 73618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.001"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..309
                     /locus_tag="ACHBRQ_000001"
     CDS             <1..309
                     /locus_tag="ACHBRQ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9591770.1"
                     /translation="ENTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSQDNGMME
                     SFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG
                     "
     gene            complement(437..685)
                     /locus_tag="ACHBRQ_000002"
     CDS             complement(437..685)
                     /locus_tag="ACHBRQ_000002"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9591771.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(633..1715)
                     /locus_tag="ACHBRQ_000003"
     CDS             complement(633..1715)
                     /locus_tag="ACHBRQ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9591772.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1841..2623)
                     /gene="gatD"
                     /locus_tag="ACHBRQ_000004"
     CDS             complement(1841..2623)
                     /gene="gatD"
                     /locus_tag="ACHBRQ_000004"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HFM9591773.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2623..3966)
                     /gene="murT"
                     /locus_tag="ACHBRQ_000005"
     CDS             complement(2623..3966)
                     /gene="murT"
                     /locus_tag="ACHBRQ_000005"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HFM9591774.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4065..5381)
                     /locus_tag="ACHBRQ_000006"
     CDS             complement(4065..5381)
                     /locus_tag="ACHBRQ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9591775.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5575..6801
                     /locus_tag="ACHBRQ_000007"
     CDS             5575..6801
                     /locus_tag="ACHBRQ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFM9591776.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGILGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7000..8574
                     /locus_tag="ACHBRQ_000008"
     CDS             7000..8574
                     /locus_tag="ACHBRQ_000008"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9591777.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8838..9626
                     /gene="codY"
                     /locus_tag="ACHBRQ_000009"
     CDS             8838..9626
                     /gene="codY"
                     /locus_tag="ACHBRQ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9591778.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9626..10201
                     /locus_tag="ACHBRQ_000010"
     CDS             9626..10201
                     /locus_tag="ACHBRQ_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9591779.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10283..10368
                     /locus_tag="ACHBRQ_000011"
     tRNA            10283..10368
                     /locus_tag="ACHBRQ_000011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10317..10319,aa:Leu,seq:aag)
     gene            10651..11781
                     /locus_tag="ACHBRQ_000012"
     CDS             10651..11781
                     /locus_tag="ACHBRQ_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591780.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(11928..12074)
                     /locus_tag="ACHBRQ_000013"
     CDS             complement(11928..12074)
                     /locus_tag="ACHBRQ_000013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591781.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            12282..13040
                     /locus_tag="ACHBRQ_000014"
     CDS             12282..13040
                     /locus_tag="ACHBRQ_000014"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9591782.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13128..13640
                     /locus_tag="ACHBRQ_000015"
     CDS             13128..13640
                     /locus_tag="ACHBRQ_000015"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9591783.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13822..14766
                     /gene="metA"
                     /locus_tag="ACHBRQ_000016"
     CDS             13822..14766
                     /gene="metA"
                     /locus_tag="ACHBRQ_000016"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFM9591784.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAARQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14775..15452
                     /locus_tag="ACHBRQ_000017"
     CDS             14775..15452
                     /locus_tag="ACHBRQ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9591785.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15550..16308
                     /gene="tpiA"
                     /locus_tag="ACHBRQ_000018"
     CDS             15550..16308
                     /gene="tpiA"
                     /locus_tag="ACHBRQ_000018"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9591786.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16346..17851
                     /gene="lytC"
                     /locus_tag="ACHBRQ_000019"
     CDS             16346..17851
                     /gene="lytC"
                     /locus_tag="ACHBRQ_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HFM9591787.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18349..18867
                     /locus_tag="ACHBRQ_000020"
     CDS             18349..18867
                     /locus_tag="ACHBRQ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9591788.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            18997..19503
                     /locus_tag="ACHBRQ_000021"
     CDS             18997..19503
                     /locus_tag="ACHBRQ_000021"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9591789.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19503..19673
                     /locus_tag="ACHBRQ_000022"
     CDS             19503..19673
                     /locus_tag="ACHBRQ_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591790.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19705..20937
                     /gene="clpX"
                     /locus_tag="ACHBRQ_000023"
     CDS             19705..20937
                     /gene="clpX"
                     /locus_tag="ACHBRQ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9591791.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20946..21533
                     /gene="yihA"
                     /locus_tag="ACHBRQ_000024"
     CDS             20946..21533
                     /gene="yihA"
                     /locus_tag="ACHBRQ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9591792.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21544..21924
                     /locus_tag="ACHBRQ_000025"
     CDS             21544..21924
                     /locus_tag="ACHBRQ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFM9591793.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21976..22866
                     /gene="rapZ"
                     /locus_tag="ACHBRQ_000026"
     CDS             21976..22866
                     /gene="rapZ"
                     /locus_tag="ACHBRQ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9591794.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22863..23840
                     /locus_tag="ACHBRQ_000027"
     CDS             22863..23840
                     /locus_tag="ACHBRQ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HFM9591795.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23837..24748
                     /gene="whiA"
                     /locus_tag="ACHBRQ_000028"
     CDS             23837..24748
                     /gene="whiA"
                     /locus_tag="ACHBRQ_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9591796.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24782..25750)
                     /locus_tag="ACHBRQ_000029"
     CDS             complement(24782..25750)
                     /locus_tag="ACHBRQ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9591797.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25886..26743
                     /gene="cdaA"
                     /locus_tag="ACHBRQ_000030"
     CDS             25886..26743
                     /gene="cdaA"
                     /locus_tag="ACHBRQ_000030"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9591798.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26730..27509
                     /locus_tag="ACHBRQ_000031"
     CDS             26730..27509
                     /locus_tag="ACHBRQ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9591799.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27533..28885
                     /gene="glmM"
                     /locus_tag="ACHBRQ_000032"
     CDS             27533..28885
                     /gene="glmM"
                     /locus_tag="ACHBRQ_000032"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9591800.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVNYYVDTITDVVRAEIGID
                     "
     gene            28959..29333
                     /locus_tag="ACHBRQ_000033"
     CDS             28959..29333
                     /locus_tag="ACHBRQ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9591801.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29335..30183
                     /locus_tag="ACHBRQ_000034"
     CDS             29335..30183
                     /locus_tag="ACHBRQ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9591802.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30449..31216
                     /gene="dapB"
                     /locus_tag="ACHBRQ_000035"
     CDS             30449..31216
                     /gene="dapB"
                     /locus_tag="ACHBRQ_000035"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9591803.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWIDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31213..32412
                     /locus_tag="ACHBRQ_000036"
     CDS             31213..32412
                     /locus_tag="ACHBRQ_000036"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9591804.1"
                     /translation="MRLTQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAI
                     TEKYQSLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            32409..34280
                     /locus_tag="ACHBRQ_000037"
     CDS             32409..34280
                     /locus_tag="ACHBRQ_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9591805.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKSDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLNKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34423..35607)
                     /gene="mntE"
                     /locus_tag="ACHBRQ_000038"
     CDS             complement(34423..35607)
                     /gene="mntE"
                     /locus_tag="ACHBRQ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HFM9591806.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPILEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35911..38607
                     /locus_tag="ACHBRQ_000039"
     CDS             35911..38607
                     /locus_tag="ACHBRQ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9591807.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAMDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     LLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFASDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38620..39411
                     /gene="yghU"
                     /locus_tag="ACHBRQ_000040"
     CDS             38620..39411
                     /gene="yghU"
                     /locus_tag="ACHBRQ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFM9591808.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKVANRPAVKRGLEVTYTEIK"
     gene            39527..39937
                     /locus_tag="ACHBRQ_000041"
     CDS             39527..39937
                     /locus_tag="ACHBRQ_000041"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9591809.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39949..41544
                     /locus_tag="ACHBRQ_000042"
     CDS             39949..41544
                     /locus_tag="ACHBRQ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591810.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            41711..41875
                     /locus_tag="ACHBRQ_000043"
     CDS             41711..41875
                     /locus_tag="ACHBRQ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HFM9591811.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            41954..42682
                     /gene="yaaA"
                     /locus_tag="ACHBRQ_000044"
     CDS             41954..42682
                     /gene="yaaA"
                     /locus_tag="ACHBRQ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFM9591812.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42960..43508)
                     /locus_tag="ACHBRQ_000045"
     CDS             complement(42960..43508)
                     /locus_tag="ACHBRQ_000045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9591813.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            43829..44317
                     /locus_tag="ACHBRQ_000046"
     CDS             43829..44317
                     /locus_tag="ACHBRQ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9591814.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            44314..45501
                     /locus_tag="ACHBRQ_000047"
     CDS             44314..45501
                     /locus_tag="ACHBRQ_000047"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9591815.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     CTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            45494..45859
                     /locus_tag="ACHBRQ_000048"
                     /pseudo
     CDS             45494..45859
                     /locus_tag="ACHBRQ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            45875..47221
                     /gene="asnS"
                     /locus_tag="ACHBRQ_000049"
     CDS             45875..47221
                     /gene="asnS"
                     /locus_tag="ACHBRQ_000049"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9591816.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            47374..47664
                     /gene="rpsF"
                     /locus_tag="ACHBRQ_000050"
     CDS             47374..47664
                     /gene="rpsF"
                     /locus_tag="ACHBRQ_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9591817.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            47676..48146
                     /gene="ssbA"
                     /locus_tag="ACHBRQ_000051"
     CDS             47676..48146
                     /gene="ssbA"
                     /locus_tag="ACHBRQ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HFM9591818.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            48178..48417
                     /gene="rpsR"
                     /locus_tag="ACHBRQ_000052"
     CDS             48178..48417
                     /gene="rpsR"
                     /locus_tag="ACHBRQ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9591819.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            48712..50112
                     /locus_tag="ACHBRQ_000053"
     CDS             48712..50112
                     /locus_tag="ACHBRQ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HFM9591820.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            50114..50473
                     /locus_tag="ACHBRQ_000054"
     CDS             50114..50473
                     /locus_tag="ACHBRQ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFM9591821.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            50527..51276
                     /locus_tag="ACHBRQ_000055"
     CDS             50527..51276
                     /locus_tag="ACHBRQ_000055"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9591822.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            51266..51565
                     /locus_tag="ACHBRQ_000056"
     CDS             51266..51565
                     /locus_tag="ACHBRQ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9591823.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            51636..52571
                     /locus_tag="ACHBRQ_000057"
     CDS             51636..52571
                     /locus_tag="ACHBRQ_000057"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9591824.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            52657..53169
                     /locus_tag="ACHBRQ_000058"
     CDS             52657..53169
                     /locus_tag="ACHBRQ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HFM9591825.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            53153..53802
                     /locus_tag="ACHBRQ_000059"
                     /pseudo
     CDS             53153..53802
                     /locus_tag="ACHBRQ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            53903..54097
                     /locus_tag="ACHBRQ_000060"
     CDS             53903..54097
                     /locus_tag="ACHBRQ_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9591826.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVSKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(54143..55588)
                     /locus_tag="ACHBRQ_000061"
     CDS             complement(54143..55588)
                     /locus_tag="ACHBRQ_000061"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9591827.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYIAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISLDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FRFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            55694..57316
                     /locus_tag="ACHBRQ_000062"
     CDS             55694..57316
                     /locus_tag="ACHBRQ_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9591828.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            57519..59477
                     /locus_tag="ACHBRQ_000063"
     CDS             57519..59477
                     /locus_tag="ACHBRQ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9591829.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKSKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            59649..60605
                     /locus_tag="ACHBRQ_000064"
     CDS             59649..60605
                     /locus_tag="ACHBRQ_000064"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591830.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFEGGKSVSG"
     gene            60609..61331
                     /locus_tag="ACHBRQ_000065"
     CDS             60609..61331
                     /locus_tag="ACHBRQ_000065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591831.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGKKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            61488..62582
                     /locus_tag="ACHBRQ_000066"
     CDS             61488..62582
                     /locus_tag="ACHBRQ_000066"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9591832.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            62590..63756
                     /locus_tag="ACHBRQ_000067"
     CDS             62590..63756
                     /locus_tag="ACHBRQ_000067"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9591833.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSIKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            63849..66947
                     /locus_tag="ACHBRQ_000068"
     CDS             63849..66947
                     /locus_tag="ACHBRQ_000068"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9591834.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREKDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            66995..67612
                     /locus_tag="ACHBRQ_000069"
     CDS             66995..67612
                     /locus_tag="ACHBRQ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9591835.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKLVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            67623..68957
                     /gene="murC"
                     /locus_tag="ACHBRQ_000070"
     CDS             67623..68957
                     /gene="murC"
                     /locus_tag="ACHBRQ_000070"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9591836.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            68967..69431
                     /locus_tag="ACHBRQ_000071"
     CDS             68967..69431
                     /locus_tag="ACHBRQ_000071"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9591837.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKVIRLESPDELLSYFGMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            69428..69922
                     /locus_tag="ACHBRQ_000072"
     CDS             69428..69922
                     /locus_tag="ACHBRQ_000072"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9591838.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVDLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            70003..71658
                     /gene="mltG"
                     /locus_tag="ACHBRQ_000073"
     CDS             70003..71658
                     /gene="mltG"
                     /locus_tag="ACHBRQ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9591839.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPAKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            71729..72211
                     /gene="greA"
                     /locus_tag="ACHBRQ_000074"
     CDS             71729..72211
                     /gene="greA"
                     /locus_tag="ACHBRQ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9591840.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(72268..72636)
                     /locus_tag="ACHBRQ_000075"
     CDS             complement(72268..72636)
                     /locus_tag="ACHBRQ_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591841.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKHGFEVVDETDEVS
                     SKHGFEVVDETDEVSSKHGFEVVDETDEVGSKHGFEVVDETDEVSSKHCFEVVDETDK
                     VSNHIYGKATLTWFEEIFEE"
     gene            72764..73162
                     /locus_tag="ACHBRQ_000076"
     CDS             72764..73162
                     /locus_tag="ACHBRQ_000076"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9591842.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT        21826 a        13718 c        16671 g        21403 t
ORIGIN      
        1 gagaatacga ttctccatag tgaccaaggc tggcaatacc gacacgattc ttatcatcgg
       61 ttcctagaga gtaagggagt tcaagcatcc atgtcacgca agggaaacag ccaagacaac
      121 ggtatgatgg aatctttctt tgggattctg aaatcggaaa tgttttacgg ttatgagaag
      181 tcgtttcagt cgcttaagca attggaacaa gctattgtag actatattga ttactacaac
      241 aataaacgaa ttaaggtaaa actaaaagga cttagtcctg tgcaatacag aactaaatcc
      301 ttcggataaa ttatttgtct aacttttggg gggcagtaca ttttaacact tgatactatg
      361 cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt tctaacatct
      421 tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg ggtttcatcc
      481 cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt agggttagat
      541 tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata gcccctagat
      601 attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa atctttgctg
      661 gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc aatacgaaca
      721 ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc ttcttcgatg
      781 accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc gataccatgc
      841 ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc acgggcagcg
      901 cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc ttgttgggct
      961 tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac agcgactgta
     1021 cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag gagaagagca
     1081 tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc
     1141 aagaccatgg tatcaaagct catttcataa ccttcacgtt tcatggcaaa gtcgatttgt
     1201 gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac atgaacagcc
     1261 ttgtcagcat aaagacctgc aaccatcatt ttttgcactt catcagctga tttgatgagg
     1321 cgcatgcgtt ggatacgagg agtgaggttg tcaaactcag cagtctcaaa aactgttttc
     1381 aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg tttgaagtca
     1441 agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac atagcccact
     1501 actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac aaagaggaga
     1561 ggttcctgat ccgctaggac aaagaggaac atttggcgtt catggggatc actgtaaaaa
     1621 ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag tttttctgat
     1681 tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc tacccctcta
     1741 ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga caaaaaactc
     1801 cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag tcagccttgc
     1861 ttttgacgtc actgtactct tcagcgattt cttggctgag aatgtcctca taggcaggga
     1921 gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga taagccagat
     1981 tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc ttataatgaa
     2041 ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt cccagcggtt
     2101 tttggtcatc agagaggaag gtacgacctt ggtgattttc aaatccatag taggtttcat
     2161 cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc tggttgagcg
     2221 tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca acataatatt
     2281 gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt tggatgtagt
     2341 tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt
     2401 gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca tggagagaaa
     2461 cgatgtcaac ggtcacatgg gctcccagtt tttcagccac atacttgagc atgaggatgt
     2521 ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg ttgagctgat
     2581 aggggtaatt gctatctttt gaggaaagtg aagtataaac cattagttca tctcctttct
     2641 aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat aagttgccag
     2701 aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct ccagattact
     2761 cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag cgatttcaga
     2821 atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt gctcaaagtc
     2881 tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa ggaggacaga
     2941 taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg caccgactgg
     3001 atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt cttggcgtcc
     3061 aaagacagca cggctcttgt caaatccctg tttgatgagt tgggaatctg cgccgaggaa
     3121 acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc cgatttggat
     3181 accgtattct tggccgtcta tgacaaagcg agagcgattg ttggtcaact caaccagttt
     3241 tgtcaaacga tagtcgagat caggacgttt acatccacaa ccttcacaga tataggcacc
     3301 caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag gacagagaat
     3361 cccttcggta ttgtagtgag caagttgggc tggtcctttt tccaagtcaa aaccaaaata
     3421 ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt taaggagaac
     3481 agtagcagtt ggaacttttc gaatggcatc caatatcatg ttataggtag tatagatttc
     3541 accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac taggctggat
     3601 atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa tatttttccc
     3661 agtttttgaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc
     3721 gcttgggttg gttagaactt gaccataaac ctcttttaaa atgccgacag tgagggcagt
     3781 tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct tagctaggct
     3841 ttgtaaaata tctttatcaa attgaagggc gactttccct gggagcgtac ttccacgtcc
     3901 aagacggctt aaaacgaagt gggaagaacg cccagcaaga aggcccaaag tagtttttaa
     3961 gttcatgttt ctattatatc acaaaagagg gaaaagacag atttggtgtt tcgtagaagt
     4021 aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat cgcaaacaag
     4081 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
     4141 ggaatcttgt tgtccatagc aacagtaatg atgttgatga tttcttgaga accttctgag
     4201 aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt gaaagctccg
     4261 cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc tttcactgcg
     4321 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
     4381 gtgatgaaca tagtatttgg cacattgaga cggtcctcaa gtgtgtagct gccatctcca
     4441 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca
     4501 ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttgtctact
     4561 ttaatagcac cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
     4621 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
     4681 acgacaagca cttggtcacc atcgtttttg atttcagtag tatggatatt ttgaagcaat
     4741 tcaataccgt cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
     4801 ggtaggaatg tatccaaggc atctaggact gtaaccttgc ttcctagttt attgtaaagg
     4861 ccagcaaatt caagaccgat atttccgcca ccaaggactc caagtttttc aggcaattta
     4921 tccaagcttt ggatacctgt tgagtcaaag acgtttttgc ttgtagcaag tccagggatt
     4981 ggcaagacgt ttgaaacagc accagtgttg atgacgatag tttcagcagt caattcttgt
     5041 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttcagcatca
     5101 aagatattga cgccagtacc agcaacagtc gtatagtttt taccgttgag gcgaccagtg
     5161 atcgtgtttt tggtagcaat gacttcttca aaagacaagt ctttctcagc agcaaccagc
     5221 aaagttttag ttgggataca accgatgttg atacaagttc caccgtacat agctttgcta
     5281 cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag tgttttacca
     5341 gctttaccaa atccgataac gattaaatca taagttaaca tttagagttc ctcctattcg
     5401 catttcattc tagcacaaga aaaaaacttt tgcacaattc tgctacggaa aagacagaaa
     5461 aagataaaaa cgctttccag acaaaaaact agtaattttt tgacaagctt gataaactaa
     5521 aatcctatct atttttatat aagatagaaa aaatgttcga aaataggagg ttttatgaaa
     5581 tcgaatcgtt atattattgc ctttgctggg gtcattttac acttaatgct gggttcgact
     5641 tatgcttgga gtgtttatcg taaccctatt attgaaaaaa cgggatggga tcaggcttct
     5701 gttgcctttg cctttagtct agcaatcttt tgtttgggct tatcggctgc atttatgggg
     5761 cgtttggtag aaaaatttgg tccgagagtc atggggagtc tatctgcttt tctatacgca
     5821 ggtggaaata tcttaacagg atttgcaata gaccgtcagg aactgtggtt gttgtatcta
     5881 gcttatggca ttttaggtgg gcttggtttg ggagcaggct atattacccc tgtgtcaacg
     5941 attataaaat ggtttcctga taaacgtggt ctcgcaacag gtttagcgat tatggggttt
     6001 ggttttgctt ctttattgac tagtcccata gcgcaacatc tcatcgcagg ggtagggctt
     6061 gtagaaactt tttatatttt aggtgcaagt tactttatta tcatgctcct agcttcacaa
     6121 ttcattaagc gtccaaatga gcaagagctt gcaattttat ctgcttcagg gaaagaaaaa
     6181 acagactctc tgacgcaagg gatgactgca aatcaggctc taaaaagcaa tcggttttat
     6241 atgctttgga ttattttctt tatcaacata gcttgtggtt taggcttaat ttcagcggca
     6301 tcgccaatgg cacaggagat ggctggcttg tctacaagtc atgcagcagt aatggtgggt
     6361 attttgggga ttttcaatgg atttggtcgc ttgctctggg cgagtttgtc tgactatatc
     6421 ggtcgccctc taacctttag tatattactg cttgtcaatc ttttcttttc tctctcactt
     6481 tggctcttta cagattccgt tttatttgta gttgctatgt ctattttgat gacttgctat
     6541 ggagctggtt tttctttgat tccagcttat ctcagtgata tttttggaac caaggaattg
     6601 gccgctctgc atggttatat tttaacagct tgggcaatgg ctggtttagc gggacctatt
     6661 ttattagcag agacttataa aatggctcat tcgtacacac aaaccttgtt cgtttttctc
     6721 attttataca gtatcgcctt ggctttgtct tattatctag gtcgttcaat caaaaaagaa
     6781 agtcaaaaac cgcttacatg attttctgta gaaatggggc tatcttttgc tctggtcttg
     6841 tgttatacta gataggttgc aaagaaaaca gtacttttct tttgtggaaa agaagcaaca
     6901 cgattttata tctagttata tgaaaatacg tcataaaaag aaaagtataa actaagccct
     6961 atagggtggc ttcgcaccac ctttagaaag aagaataacg tgaaatttaa tgaattaaac
     7021 ttgtctgctg atttgctagc agaaattgaa aaagctggtt ttgtagaagc tagtcctatc
     7081 caagaacaaa ctattccttt ggcccttgaa ggcaaggatg ttatcggtca agctcagact
     7141 ggtacaggaa aaactgcagc ctttggcttg cctacccttg aaaaaatccg tacagaagaa
     7201 gcgactatcc aagctttggt catcgctcca actcgtgaac tagctgtcca aagtcaagaa
     7261 gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc gttcagtata tggcggatca
     7321 agcattgaaa aacaaattaa ggctcttaaa tctggtgccc atatcgtggt gggaactcca
     7381 ggtcgcctct tggacttgat taaacgcaag gccttgaaat tacaagacat tgaaaccctt
     7441 atccttgacg aagcggatga aatgcttaac atgggcttcc ttgaagacat cgaagccatt
     7501 atttcccgtg tacctgagaa ccgtcaaact ttgcttttct cagcaactat gccagatgcc
     7561 atcaaacgta tcggtgttca gtttatgaaa gcccctgaac atgtcaagat tgcggctaag
     7621 gaattgacaa cagaattggt tgaccagtac tatatccgtg ttaaggaaca agaaaaattt
     7681 gacaccatga ctcgtctcat ggatgtggca caaccagaac tcgctattgt atttggtcgt
     7741 accaaacgcc gtgtggatga attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa
     7801 ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat
     7861 ggcaatcttg atgttttggt tgcgacagac gttgcagcgc gtggtttgga tatttcaggt
     7921 gtgacccatg tctacaacta cgatattcca caagatcctg agagttatgt tcaccgtatc
     7981 ggtcgtacag gtcgtgctgg taagtcaggt caatctatta cttttgttgc tccaaacgaa
     8041 atgggttacc ttcaaatcat tgaaaacttg actaagaaac gcatgaaagg tctcaaacct
     8101 gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt
     8161 gattttgcag atgaaaccat tcgtgccaac tttgagaaat ttggtaagga tgctcgcaaa
     8221 ttggctgctg agtttactcc agaagaattg gcaatgtata tcttgagtct gacagtccaa
     8281 gacccagata gccttccaga agtggagatt gcacgtgaaa aaccactacc gtttaaacca
     8341 tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg gaggccgtcg tggggacgac
     8401 cgtcgagagc gtgatcgccg tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt
     8461 ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg ataataaaaa accacgcaat
     8521 actttaagcg aaaagcaaac aggctttgtt attcgtaaca agggcgataa ataagaaaaa
     8581 gcggttcaaa atgaatcgct tttttatata aggagaccga atgtgaaaag acaaataaaa
     8641 ttattatatt atcttttata atgttatgat acacaaaaat aggcaacttg tcaatagaaa
     8701 aggaacaatt taattaaaaa tattctggtt tttaattaaa ttgtaagtca atgcaagtca
     8761 ataaggaatt ttcagataat tattgaaaaa gagacgtttt tatgttataa tgatagcgat
     8821 taaattcgag gtgaaaaatg gcacatttat tagaaaaaac tagaaaaatt acatcaattt
     8881 tgaaacgctc agaggagcag ttgcaggatg agctccctta caacgctatt acgcgtcagt
     8941 tagcggatat tattcattgc aatgcctgca ttatcaatag taagggacgt ctgcttggct
     9001 attttatgcg ttataaaaca aatacagatc gcgtagagca attcttccaa actaagattt
     9061 tcccagatga ctacgttcaa ggggctaata tgatttacga aacagaagca aacttgcctg
     9121 ttgagcatga tatgagtatt ttccctgttg agagtagaga tgattttcca gatggcttga
     9181 cgactattgc accgattcat gtatcgggga ttcgccttgg ttctttgatt atttggcgta
     9241 atgataaaaa attcgaagat gaggacttgg ttcttgttga gattgccagt accgttgttg
     9301 ggattcagct tcttaacttc caacgtgaag aagatgagaa aaatattcgt cgtcgtactg
     9361 ctgtcaccat ggcggtcaat accctttctt actccgaact ccgtgctgtt tcagcaattt
     9421 taggggaatt aaatggaaat gaagggaagt tgactgcgtc agtgattgca gatcgtatcg
     9481 gaatcactcg ctctgtgatt gtcaatgctc ttcgtaaact tgagtctgcg gggattattg
     9541 aaagtcgctc acttggaatg aagggaacct atcttaaggt cttgatttca gatatttttg
     9601 aagaagtgaa gaaaagagat tactaatgac aaaggcttta atttcgattg attatacaga
     9661 agattttgtt gctgatagtg ggaagctgac agcaggtgct ccagctcagg cgatttcgga
     9721 tgccatcagc aaggtaactc gattagcttt tgaacgagga gattatatct tctttactat
     9781 tgatgctcat gaagaaaacg attttttcca tccggaaagt aagttatttc ctcctcataa
     9841 tctgattggg acgagtggac ggaatttata tggagatttg gggatctttt atcaagagca
     9901 tggttcagac agtcgtgtct tttggatgga taaacgccat tactcagctt tttcagggac
     9961 tgacctagat attcgtttga gagagcgtag agtgtctacc gttatcttaa caggtgtctt
    10021 gacggatatc tgtgtcctac atacagctat agatgcctat aatctaggat atgacatcga
    10081 gattgttaaa ccagctgttg cttccatctg gcctgaaaat catcaatttg ccctaggtca
    10141 tttcaaaaat acactcggag ctaagttagt agatgaaaat ctaaatgaac tttctgagta
    10201 acctggttgg tgaaaatgaa aaaatttgaa aaaaagtgtt gacaagcatc ccaaaagttg
    10261 atatactagt aaagtaatcg atgcggggat ggcggaattg gcagacgcgc aggactaagg
    10321 atcctgtgac cgctttaggt cgtgagggtt caagtccctc tctccgcata ggaaaagagt
    10381 tagctaaagc tagctctttt gtatttctct aaaaaattag aatccagtca aatgtagtgg
    10441 agaatcagcg aaaatatggg aaaactgatc cttgtttctg cttttgtcat agaagaataa
    10501 taaagattat cttcttcaaa ttctccgata ttctctaaag ttttgtgcaa gttgcacaga
    10561 acttttttat ttttttggtc atcttgccat agaaatataa agcgttttca tctataatat
    10621 aattatcaaa agacaaaagg agttcacctc atggtagaat tgaatcttaa aaatatttac
    10681 aaaaaatatc caaacagcga acactattca gttgaagatt tcaacttgaa catcaaagat
    10741 aaagaattta tcgttttcgt aggaccttca ggatgtggta aatcaactac actccgtatg
    10801 attgctggtc ttgaagacat tacagaaggt actgcatcta tcgatggcgt ggttgtcaac
    10861 gacgtagctc caaaagaccg tgatatcgcc atggtattcc aaaactacgc tctttaccca
    10921 cacatgactg tttatgacaa catggctttc ggtttgaaat tgcgtaaata cagcaaagaa
    10981 gacattaaca aacgtgttca agaagcagct gaaatacttg gattgaaaga attcttggaa
    11041 cgtaaaccag ctgacctttc aggtggtcaa cgtcaacgtg ttgccatggg gcgtgcgatt
    11101 gtccgtgatg cgaaagtatt cttgatggac gaacctttgt caaacttgga tgccaaactt
    11161 cgtgtatcaa tgcgtgctga aatcgctaaa attcaccgtc gtatcggagc tacaactatc
    11221 tatgtaactc acgaccaaac agaagcgatg acacttgcag accgtatcgt tattatgtca
    11281 gctactaaga accctgctgg tacaggtact atcggacgtg tagaacaaat cggtactcct
    11341 caagaagttt acaaaaatcc agttaacaaa tttgttgcag gattcatcgg aagcccagct
    11401 atgaatttca tcaacgtgaa attggttggt agcgaaattg tttctgacgg tttccgtttg
    11461 aaagtgccag aaggagcatt gaaagttctt cgtgaaaaag gctacgaagg aaaagaattg
    11521 atctttggta tccgtccaga agacgtgaat gcagaacctg ctttccttga aacattccca
    11581 gactgtgttg taaaagcgac tatctctgta tcagaactgc ttggttcaga atctcacctt
    11641 tactgccaag ttggtaaaga cgagtttgtt gcaaaagttg atgctcgtga ctacttgcaa
    11701 acaggtgcaa cagttgagct tggatttgac ttgaacaaag cacacttctt cgatgtagaa
    11761 actgaaaaaa caatctacta aaataaataa aattcaaagc actacaagaa aagatatctc
    11821 tttatcaatt gtagtggaga gatatcagtt aatctaggga gagaaacaaa atgcttctct
    11881 cctttttgct agggaagtca tattatgcat ctatattgtg atgctcttta atactcttcg
    11941 aaaatctctt caaaccacgt caacgtcgcc ttgccgtacg tatgattact gatttcgtca
    12001 gttttatctg caacctcaaa gatgtacttt gagcagctta cggctagttt cctagtttgc
    12061 tctttgattt ccattgagta ttatttgtgg gtaccatcta caagtgaagc tatatgcgta
    12121 aactacgtga gcaattgaat tcgaactaga gaggtaataa taaatttatg ctatagttat
    12181 ggtgacttgt atgcttttga ttctagttta tcaaataata gattagaatt gtcagataat
    12241 atcattttgt gttataatga agaaaaaaca gaggtgttca aatgtcagaa gcaggtcata
    12301 agtttttagc aaaattgggg aaaaaacgct tacgtccagg tggaaagcgt gccacagatt
    12361 ggttaattgc agaaggagga ttttcaaaag aaaagagaat actagaggtt gcgtgtaata
    12421 ggggaactac agcaattgag ttggcacagc gttttggttg taagataact gctgttgata
    12481 tggatgctca agctttagaa gtggctaaaa aatctgctgg aacggcaggt gttgctcatt
    12541 taatcagttt tgaaagagca aatgcaatga aacttcctta tcaagatgct agttttgata
    12601 ttgttataaa tgaagctatg ctgactatgc aagccgatca agctaagaaa aaatgtgtaa
    12661 tggaatatct aagggtatta aaacctggag gtcttctctt gacacatgat gtgcttctta
    12721 aggaagctaa agagtctatc agacaggaat tatcacaagc aattcatgta aatgtaggtc
    12781 ctttaactca agatggttgg gaacaggtga tgatagaatc aggttattgt gatgtgaaag
    12841 cattgactgg tgaaatgaca ttaatgaaat tatcgggtat gatttatgac gaaggtttgc
    12901 taggaacttt gaaaatttgt gtaaatgctt gtaaaaagga gaatagaaag cagtttttaa
    12961 ctatgtataa aatgtttgct aagaataaac agaaattggg ctttattgcg atggctagtt
    13021 ataaatcgtc aaaacgttag ataattattg aagttaactt ttcctttttt ctttcttaaa
    13081 aaatatgcta taatagagag taaaaaactt tgaaagaaag aaaaaagatg aatttaaaag
    13141 attacattgc aacaattgaa aattatccaa aggaaggcat taccttccgt gatattagtc
    13201 ctttgatggc tgatggaaat gcttatagct acgctgttcg tgaaatcgtt cagtatgcta
    13261 ctgacaagaa agtcgacatg atcgtgggac ctgaagctcg tggatttatc gtgggttgtc
    13321 cagttgcctt tgagttggga attggttttg cgcctgttcg taagccaggt aaattgccac
    13381 gcgaagttat ttctgctgac tatgaaaaag agtacggtgt cgataccttg actatgcacg
    13441 cggatgccat taagccaggt caacgtgttc ttattgtaga tgaccttttg gcgacaggtg
    13501 gaactgttaa ggcaactatc gagatgattg aaaaacttgg tggtgttatg gcaggttgtg
    13561 ccttccttgt tgaattggat gaattgaacg gccgtgaaaa aatcggtgac tacgactaca
    13621 aagttcttat gcattattaa tgaaaacagt ccctagggct gttttctcta cactaggata
    13681 taaaaataga ctataactag ttagagaaaa actataattg aaaactatat cttcttgcag
    13741 tataataaaa ggactaagtg tttgagattt gtcttcaaac acatgcaatt attcctgaga
    13801 gagtacagtt aggagagggt tatgccgatt cgaattgata aaaaattgcc agctgttgag
    13861 attttacgga cagagaatat ctttgtcatg gatgatcaac gtgctgcccg ccaagatatc
    13921 cgtcctttga agattttaat tttaaatctc atgccacaga aaatggtcac agagacccag
    13981 ttgttgcgcc acttggctaa tacaccccta caactggata ttgattttct ctatatggag
    14041 agccaccgtt ctaaaacaac tcgttcagag cacatggaga ccttctataa aacttttcct
    14101 gaagtcaagg atgagtattt tgatgggatg atcatcacgg gtgctccagt tgagcattta
    14161 ccatttgagg aagtggacta ttgggaggaa tttagacaga tgcttgagtg gtctaagact
    14221 catgtctatt cgacccttca tatctgttgg ggagctcagg ctgggcttta tctgcgctat
    14281 ggtgtagaaa aataccagat ggacagtaag ctatcaggta tttatcctca ggacacccta
    14341 aaagagggtc accttctatt tagaggcttt gatgatagct atgtatcccc tcattcacgg
    14401 cacacggaga tttctaagga agaggtctta aacaagacta atctcgagat tttatcagaa
    14461 ggacctcagg ttggggtttc tattttggcc agtcgtgatt tacgagaaat ttatagtttt
    14521 ggtcatttgg agtatgaccg tgataccttg gcaaaagagt attttcgaga tcgtgatgca
    14581 ggtttcgacc cacatattcc agaaaattac tttaaagatg atgatgttaa tcaggtacct
    14641 tgtctttgtt ggtcttcatc tgcagccctc tttttcagta attgggtaaa ccatgcggtc
    14701 tatcaggaga cgccttttga ctggagaaag atagaagatg atgcatctgc atatgggtat
    14761 ttataagagg aattatgaca tatttagacg cttttaaatc aggtaccttg gttttaccga
    14821 gtgccctgct cttgcatttt aaggaactct ttccttctag cgacgatttt ctggtttggc
    14881 aatttttcta tttgcaaaat acgacaggct tagaagaaat gtcgccaagc cagattgctg
    14941 aaaggattgg caaggaaatt tcggatgtca accagtccat ttctaatctg acggaaaggg
    15001 gactgctcca gtatcgtact atcgaattaa atggcgaaat tgaattgctc tttgatgcta
    15061 gtttggcctt ggaacgtttg gatgacctgt ttggagcagt tcattcaagt tcagaccagc
    15121 taacaccaca aaaccagctc aaggatttgg tggaaacctt ccagcaggag ttgggacgat
    15181 tgttgacgcc ttttgagatt gaggatttga ccaagacact aaaggaagat ggaaccagtg
    15241 ctgacttgat taaggaggct cttcgtgaag ctgttttgaa tggaaaacca aactggaagt
    15301 acattcaggc gattttgaga aactggcgcc atgaaggaat caagagtgtg gctcaaattg
    15361 aggccaagag agcagaaaga gaagcaagca atcctcagtt gacacaggta tctgcagatt
    15421 tcagaaatgc catggatctc tggaaggatt aatccatgca agtaggcttg aaatccgagt
    15481 aagatttgca agctgtgtat aattgtgata gaataaatag aaaataaatt gaaaaaagag
    15541 gtatgtgaaa tgtcacgtaa accatttatc gctggtaact ggaaaatgaa caaaaatcca
    15601 gaagaagcta aagcattcgt tgaagcagtt gcatcaaaac ttccttcatc agatcttgtt
    15661 gaagcaggta tcgctgctcc agctcttgat ttgacaactg ttcttgctgt tgcaaaaggc
    15721 tcaaacctta aagttgctgc tcaaaactgc tactttgaaa atgcaggtgc tttcactggt
    15781 gaaactagcc cacaagtttt gaaagaaatc ggtactgact acgttgttat cggtcactca
    15841 gaacgccgtg actacttcca tgaaactgac gaagatatca acaaaaaagc aaaagcaatc
    15901 tttgcgaacg gtatgcttcc aatcatctgt tgtggtgaat cacttgaaac ttacgaagct
    15961 ggtaaagctg ctgaattcgt aggtgctcaa gtatctgctg cattggctgg attgactgct
    16021 gaacaagttg ctgcctcagt tatcgcttat gagccaatct gggctatcgg tactggtaaa
    16081 tcagcttcac aagacgatgc acaaaaaatg tgtaaagttg ttcgtgacgt tgtagctgct
    16141 gactttggtc aagaagtcgc agacaaagtt cgtgttcaat acggtggttc tgttaaacct
    16201 gaaaatgttg cttcatacat ggcttgccca gacgttgacg gtgcccttgt aggtggtgcg
    16261 tcacttgaag ctgaaagctt cttagctttg cttgactttg taaaataatc agtaagtagc
    16321 aaaagctagg tggaacagca ttcagatgtc tgttacattt tttataggag agcaaagatt
    16381 gaaaacaaaa attggattag caagtatctg tttactaggc ttggcaacta gtcatgtcgc
    16441 tgcaaatgaa actgaagtag caaaaacttc gcaggataca acgacagctt caagtagttc
    16501 agagcaaaat cagtcttcta ataaaacgca aacgagcgca gaagtacaga ctaatgctgc
    16561 tgcccactgg gatggggatt attatgtaaa ggatgatggt tctaaagctc aaagtgaatg
    16621 gatttttgac aactactata aggcttggtt ttatattaat tcagatggtc gttactcgca
    16681 gaatgaatgg catggaaatt actacctgaa atcaggtgga tatatggccc aaaacgagtg
    16741 gatctatgac agtaattaca agagttggtt ttatctcaag tcagatgggg cttatgctca
    16801 tcaagaatgg caattgattg gaaataagtg gtactacttc aagaagtggg gttacatggc
    16861 taaaagccaa tggcaaggaa gttatttctt gaatggtcaa ggagctatga tgcaaaatga
    16921 atggctctat gatccagcct attctgctta tttttatcta aaatccgatg gaacttatgc
    16981 taaccaagag tggcaaaaag tgggcggcaa atggtactat ttcaagaagt ggggctatat
    17041 ggctcggaat gagtggcaag gcaactacta tttgactgga agtggtgcta tggcgactga
    17101 cgaagtgatt atggatggtg ctcgctatat ctttgcggcc tctggtgagc tcaaagaaaa
    17161 gaaagatttg aatgtcggct gggttcacag agatggtaaa cgctatttct ttaataatag
    17221 agaagaacaa gtgggaaccg aacatgctaa gaaaatcatt gatattagtg agcacaatgg
    17281 tcgtatcaat gattggaaaa aggttattga tgagaacgag gtggatggtg tcattgttcg
    17341 tctaggttat agcggtaaag aagacaagga attggcgcat aacattaagg agttaaaccg
    17401 tctgggaatt ccttatggtg tctatctcta tacctatgct gaaaatgaga ccgatgctga
    17461 gaatgacgct aaacagacca ttgaacttat aaagaaatac aatatgaacc tgtcttaccc
    17521 tatctattat gatgttgaga actgggaata tgtaaataag agcaagagag ctccaagtga
    17581 tacagacact tgggttaaaa tcatcaacaa gtacatggac acgatgaagc aggcgggtta
    17641 tcaaaatgtg tatgtctata gctatcgtag tttattacag acgcgtttaa aacacccaga
    17701 tattttaaaa catgtaaact gggtagcggc ttatacgaat gctttagaat gggaaaaccc
    17761 ttattattca ggagaaaaag gttggcaata tacctcttct gaatacatga aaggaatcca
    17821 agggcgcgta gatgtcagcg tttggtatta agcgatgatt tgaaagaggg atgtgatagt
    17881 agcaccctct ttttctttgt tttatgatag ttcatcctcg agtaaattca agatcttgct
    17941 cggaaatgaa gcttatatag tagattgaat atagacaaat accttgtgat tggtaaaaca
    18001 ttttagaaat tcatttacct ttcctaatcg acttggtttc atcttatttc aatctattat
    18061 agtattgggg aatttcttca aaccacatca gcttggtcag ttctacctgc gacctcaaaa
    18121 cttgtgcttt ggtcaagctg ggtttagttt cctagtttgc tgatggattt ccattgacta
    18181 taagcatcca accctctttt tgtcttctaa agaattctta aattatcagt ctattgcaac
    18241 ttttctcatg tgggtcattt ggcttgcaat tgtctttcct tagtagtata ctgaggcagt
    18301 aatcattaag aagtggttac ataaaaataa tgaatgaggt aaaataaaat ggtagaattg
    18361 aaaaaagaag cagcaaaaga tgtcgcaaca ttaacaaaag cagcgccagt agcattggca
    18421 aaaacaaagg aagttttgaa tcaagcagtt gctgatttgt atgtagctca cgttgctttg
    18481 caccaagtgc actggtatat gcatggtcgt ggtttccttg tatggcatcc aaaaatggat
    18541 gagtacatgg aagctcttga tggtcattta gatgaaatca gcgaacgctt gatcacactt
    18601 ggtggaagcc cattctctac tttgacagag ttccttcaaa acagtgaaat cgaagaagaa
    18661 gctggtgaat accgtaatgt tgaagaaagc ttggaacgtg ttctagctat ttatcgctac
    18721 ctcatcactc ttttccaaaa agcgttggat gtgactgacg aagaaggcga tgatgttaca
    18781 aacgatattt ttgttggtgc taaggctgaa cttgagaaaa cagtttggat gcttgccgca
    18841 gaacttggac aagcacctgg tttgtaagaa acaagacaac atagataaat ctgtaggaaa
    18901 tttcttacag attttttcta ttttgtaagc tattccaaac cagtcttttt ttgagttttt
    18961 tgataaaata gtactatcag tgaaaaggat ggaagcatga ctaagaaaat cgtagctatt
    19021 tgggcccagg atgaagaggg tgtgattggt aaggacaatc gtctgccttg gcatttgcca
    19081 gcagaattgc agcactttaa agaaacaact ctgaatcatg ctatcttgat ggggcgggta
    19141 acctttgatg gaatggggcg tcgcttgctt ccaaaacggg agaccctgat tttgacgcgt
    19201 aatccggaag aaaagataga tggggttgct acttttcagg acgtccagtc tgttcttgac
    19261 tggtatcagg ctcaagaaaa gaatctctac attatcggtg ggaagcaaat ttttcaggct
    19321 tttgaagctt accttgatga agtgattgtc actcacattc atgctcgggt ggaaggagat
    19381 acctatttcc ctgaagagct tgacttgtct ctttttgaga cagtttcaag caaattttac
    19441 gccaaagatg agaagaatcc ttatgatttt accatccaat accgcaagag aaaggaagtt
    19501 taatggaacg gagtttattt ggtctattca cggcttttct gtgtttcatc tgctttctgg
    19561 caggagcaca agcttttcgt aaaaagcgtt acgggctttc tatcctcctt tggttaaatg
    19621 cctttaccaa tctggtcaat agtattcatg ctttttacat gaccttattt tagatagaat
    19681 gacagtatag aatggaagga aatcatgtct acaaatagaa aaaatgatat gatggtttat
    19741 tgctcatttt gtggcaaaaa ccaagaagaa gtacaaaaaa taattgctgg caacaatgct
    19801 tttatttgta atgaatgcgt ggagttagct caggaaatca ttcgagaaga attggttgag
    19861 gaagtcttgg cagacttgtc tgaggtgcca aaaccaattg aactcctcca tatcttgaac
    19921 cactatgtaa ttggtcaaga tcgtgccaag cgtgccttgg cagtggcggt ttataaccac
    19981 tacaaacgca tcaatttcca cgatacacgc gaagagtcag aagatgtgga tttgcagaag
    20041 tcaaacattt tgatgattgg cccaactggt tcagggaaaa ctttccttgc ccagaccttg
    20101 gctaagagct tgaatgtacc ttttgctatt gcggatgcga cagctctgac ggaggctggt
    20161 tatgtgggtg aggatgtgga aaatatactc ctcaaactct tgcaggttgc tgactttaac
    20221 atcgaacgtg cagagcgtgg cattatctat gtggatgaaa ttgacaagat tgccaagaag
    20281 agtgagaatg tgtctatcac acgtgatgtt tctggtgaag gggtgcaaca agcccttctc
    20341 aagattatcg agggaactgt tgctagcgta ccgcctcaag gtggacgcaa acatccacaa
    20401 caagagatga ttcaagtgga tacaaaaaat atcctcttca tcgtgggtgg tgcttttgat
    20461 ggtattgaag aaattgtcaa acaacgtctg ggtgaaaaag tcatcggatt tggtcaaaac
    20521 aataaggcga ttgacgaaaa cagctcatac atgcaagaaa tcatcgctga agacattcaa
    20581 aaatttggta ttatccctga gttgattgga cgcttgcctg tttttgcggc tcttgagcaa
    20641 ttgaccgttg atgacttggt tcgcatcttg aaagagccaa gaaatgcctt ggtgaaacaa
    20701 taccaaacct tgctttctta tgatgatgtt gagttggaat ttgacgacga agcccttcaa
    20761 gagattgcta ataaagcaat cgaacggaag acaggggcgc gtggacttcg ctccatcatc
    20821 gaagaaacca tgctagatgt tatgtttgag gtgccgagtc aggaaaatgt gaaattggtt
    20881 cgcatcacta aagaaactgt cgatggaacg gataaaccga tcctagaaac agcctagagg
    20941 tgactatgga acttaataca cacaatgctg aaatcttgct cagtgcagct aataagtccc
    21001 actatccgca ggatgaactg ccagagattg ccctagcagg gcgttcaaat gttggtaaat
    21061 ccagctttat caacactatg ttgaaccgta agaatctcgc ccgtacatca ggaaaacctg
    21121 gtaaaaccca gctcctgaac ttttttaaca ttgatgacaa gatgcgcttt gtggatgtgc
    21181 ctggttatgg ctatgctcgt gtttctaaaa aggaacgtga aaagtggggg tgcatgattg
    21241 aggagtactt aacgactcgg gaaaatctcc gtgcggttgt cagtctagtt gaccttcgtc
    21301 atgccccgtc agcagatgat gtgcagatgt acgaatttct caagtattat gagattccag
    21361 tcatcattgt ggcgaccaag gcggacaaga ttcctcgtgg taaatggaac aagcatgaat
    21421 cagcaatcaa aaagaaatta aactttgacc cgagtgacga tttcatcctc ttttcatctg
    21481 tcagtaaggc agggatggat gaggcttggg atgcaatctt agaaaaattg tgaggaaaag
    21541 aaaatggcaa aaacaattca tacagataag gccccaaagg ctatcggacc ctatgttcaa
    21601 ggaaaaatcg ttggcaacct tttgtttgct agcggtcaag ttcccctatc ccctgaaact
    21661 ggggaaattg taggagagaa tatccaagaa cagacagagc aagtcttgaa aaacatcggt
    21721 gctattttgg cagaagcagg aacagacttt gaccatgttg tcaaaacaac ttgcttcttg
    21781 agcgatatga acgactttgt tccttttaat gaggtttacc aaacggcctt caaagaggaa
    21841 ttcccagctc gttcagctgt ggaggtagct cgtcttcctc gtgatgtaaa agtcgaaatt
    21901 gaagtcatcg cagagattgg ataagctagt tgaagtttgg tgttgccaaa cttcttttga
    21961 tataaggaga aaaagatgac aaagaaacaa cttcacttgg tgattgtgac agggatgagt
    22021 ggcgcaggga aaactgtagc cattcagtcc ttcgaggatc taggttattt caccattgat
    22081 aatatgccgc cagctctctt gcctaagttt ttgcagctgg ttgaaattaa ggaagacaat
    22141 cctaagttgg ccttggtagt ggatatgcgt agccgttctt tcttttcaga gattcaagct
    22201 gttttggatg agttggaaaa tcaagatggt ttggatttca aaatcctctt tttggatgcg
    22261 gctgataagg aattggtcgc tcgttacaag gaaaccagac ggagtcaccc actagcagca
    22321 gacggtcgta ttttagatgg aatcaagttg gaacgtgaac tcttggcacc tttgaaaaat
    22381 atgagccaaa atgtggtgga tacgactgaa ctcactccac gtgagctgcg taaaaccctt
    22441 gcagagcagt tttcagacca agaacaagcc cagtctttcc gtatcgaagt catgtctttc
    22501 ggatttaagt atggaatccc gattgatgcg gacttggtct ttgatgtccg tttcttgcca
    22561 aatccctatt atctaccaga actgagaaac caaacgggtg tggatgaacc tgtttatgat
    22621 tatgtcatga accatcctga gtcagaagac ttttatcaac atttattggc cttgattgag
    22681 ccgattctgc caagttacca aaaggaaggt aagtccgttt tgaccattgc tatgggatgt
    22741 acgggtggac aacaccgtag tgtggcattt gctaaacgct tggtgcagga cttatccaag
    22801 aattggtctg ttaatgaagg gcatcgcgac aaagaccgca gaaaggaaac ggtaaaccgt
    22861 tcatgagaaa accaaagata acggtgattg gtggagggac tggaattccc gtcattctaa
    22921 aaagtctgcg ggaaaaagat gtggaaatcg cagctatcgt gacggtggca gatgatggtg
    22981 gttcttcagg tgaactccga aaaaatatgc aacagttgac accgccaggt gatcttcgta
    23041 atgtccttgt ggccatgtcg gatatgccta agttttatga gaaggtcttt cagtatcggt
    23101 tctctgagga tgccggagcc tttgctggcc atccattggg aaatctcatc attgctggct
    23161 tgtcagaaat gcagggttca acctataatg ccatgcagtt attgagcaaa tttttccata
    23221 caacagggaa aatttatcct tccagtgacc atcctttgac ccttcatgca gtctttcagg
    23281 atgggacaga agtggctgga gagagtcata ttgtagacca tcgaggcata attgacaatg
    23341 tctatgtgac caatgcccta aacgatgata cgcctctggc cagccgtcga gtagtgcaga
    23401 ccatccttga aagtgacatg attgtcctag ggccaggttc cctctttacc tctattttgc
    23461 ccaatatcgt gattaaggaa attgggcggg ctcttttgga aactaaggca gaaattgcct
    23521 atgtctgcaa tatcatgacc caacgtgggg agacggaaca ctttacagat agcgaccacg
    23581 tggaagtctt gcatcgtcac cttggtcgcc cttttatcga cactgtcttg gtgaatattg
    23641 aaaaagtgcc tcaggaatac atgaattcca accgttttga tgaatactta gtgcaagtgg
    23701 aacacgattt tgtaggtctt tgtaagcaag tttcgcgcgt gatttcatct aacttccttc
    23761 gtctggaaaa tggcggtgcc ttccacgatg gagatttgat tgtggacgag ttgatgcgca
    23821 ttatacaggt gaaaaaatga gtttcacagt agcagtaaaa gaagaaatcc tgggccaaca
    23881 ccatctgagc tggcatgaat tatctgccat tatcaaaatg tctggtagca tcggtctctc
    23941 gacttcgggc ttgactttgt cagttgtgac agaaaatgcc aaactggccc gtcacctcta
    24001 tgagtccttt cttcatttct atgaaatcaa atctgaaatt cgtcaccacc aacgtagcaa
    24061 tcttcgcaag aatcgcgtct ataccgtttt tacagatgaa aaggtgcagg atttgttaag
    24121 tgatttgcac ttggcagact ctttctttgg cctggaaaca ggtattgatg aggcgatttt
    24181 atcggatgag gaagcaggtc gtgcctatct ctgtggcgct ttcttggcaa atgggagcat
    24241 tcgtgaccct gagtcaggca agtaccagtt ggaaatcagt tctgtttatc tggaccacgc
    24301 gcaaggaatt gcctcccttc tccaacagtt tttactggat gccaaggtgc ttgagcgcaa
    24361 gaagggggct gtgacctatc tccagagagc agaagacatt atggacttct tgatagtaat
    24421 cggagccatg caggcacgtg atgattttga gcgggttaag attttgcgag aaacccgtaa
    24481 cgacctcaat cgggctaata atgccgagac agctaatatc gctcggacag tttctgccag
    24541 catgaagacc attaacaata tcagtaaaat caaagatatc atgggcttag aaaatttgcc
    24601 agtagatttg caggaagtgg cgcaactgag gattcagcac ccagactact ctatccagca
    24661 gttagcagat agtctcagca cccctctgac caaaagtggt gtcaatcaca gactcagaaa
    24721 aatcaataag atagccgatg aattataaaa ccacgaatcc tatgtgactc gtggttcttt
    24781 tttataaact agtagagtgt tttggttgta ctttttgttc agggtcaatg tagttgatag
    24841 cgttgttgac agcagttgga gcttctccga ggcctgtcgc aatcagatca atttttccgt
    24901 catagtagca gcagtcaccg atagcataga tacctgcttg gctggattcc tgtttgctgt
    24961 tgacgataat cttgtgacgg ttgaggtcga gcccccagtt tttaaggtta ccgacagaag
    25021 atttgaaacc atagttgaca aagaggtggt ctaggtcaat ggtttcagtt tcatcagatt
    25081 tgacttttgt gatttcaagt ttatcaagtg tttttccatt tccaaggagt tggctagggg
    25141 cgaatggtgt cttgatggtt acagatgatt cttgcaaggc ttgaacactg tgttccaagg
    25201 cacggaaatt atctctgcgg tgaacaaggg tagttggtgc gattttttca aaagccaaag
    25261 cccaatccac agccgagtct cccccaccaa gaatcgtcac tttcttacca gcgtattgct
    25321 gaatgttaga aacgtggtag tggatatttt catagccctc aacaccttca agttccagcg
    25381 gacgtggttt gaaggcaccg ccacccatag cgatgatgac tgttttagtc aggtgacttc
    25441 ctttagaagt tgtgatggca aattcttctt cttgtttgtc aatctcaaga accgtttcat
    25501 tgagatgaat aggggtatca aatccattta gctgttcaat caagcggtta gtcaactctt
    25561 ctccagtcag gtttgggaag cctggtacgt ctaggatttc cttttcaggg tagagaatag
    25621 caggttgtcc acctagctgg ggaagagagt cgatgatttg aaccttggct tggcgtaggt
    25681 gggcataaaa ggctgcaaaa agcccgacag gaccaccacc cacaatggta atatcataga
    25741 gttgagacat agtttctcct ttgttttttc tagtcagttt attttatcat atttttgttc
    25801 aatgtaaaat ggactaggat agggaaaaaa atgccagaaa atggtataat aggacgaaag
    25861 tgttttgata gggaggatat cagggatgaa ttttcaacaa ttatccaatc tgcaatattg
    25921 gaccagtttg tttgcaagtc catggacgat agctatcaat ctgattgata ttttgattgt
    25981 tgcttatatt ttataccatt ttacaaaagc tattgcagga accaagatta tgattttggt
    26041 acgtggagtt ttggtgttta ttttagctca aatccttgca aatatgattg gtttgactac
    26101 gatttcttgg ttaatcaatc aaattattac ttatggggtt attgcggcgg ttgttatctt
    26161 ctctccagag attcggactg gtttggaacg tttgggaaga gcgacagatt tcttttccaa
    26221 tgcccctatt agtgctgagg aacagatgat tcgtgccttt gttaagtctg ttgaatacat
    26281 gagtcctcgt aaaatcgggg ccttggttgc tattcagcgt gtacgtacct tgcaggagta
    26341 tatttcgaca ggaattccct tggatgctaa gatttctgca gaacttctca ttaacatttt
    26401 tattcccaac actcccctac atgatggtgc ggtgattatc aaagaagaac gtatcgctgt
    26461 gacgtctgcc tatctgccct tgacaaaaaa cacagggatt tccaaggaat ttgggaccag
    26521 acaccgggcg gctatcggtt tatcagaagt ctcagatgcc ttgacttttg tcgtatcaga
    26581 ggaaacggga ggaatttcga taacctataa tggaaggttt aagcacaacc taacacttga
    26641 tgaatttgaa acagaattac gtgaaatctt acttccaaaa gaggaagtgg gtcttagttt
    26701 taaagaacga ttgctaggag gatggaaaca tgaaaaaaaa tagtttatat atcatatcct
    26761 cactcttttt tgcttgtgtc ttatttgtct atgctacggc gacgaatttt caaaacagta
    26821 ccagtgctag gcaggtaaaa acggaaacct atactaatac agtaacaaat gtccctattg
    26881 acatacgcta taatagtgat aagtatttta ttagcggttt tgcttcagaa gtatcagtgg
    26941 tcttgactgg tgcaaatcgc ctatcgctag ctagtgaaat gcaagaaagt acacgtaaat
    27001 tcaaggttac tgctgaccta acagatgccg gtgttggaac gattgaagtt cctttgagca
    27061 ttgaagattt acccaatggg ctgaccgctg tggcgactcc gcaaaaaatt acagtcaaga
    27121 ttggtaagaa ggctcagaag gataaggtaa agattgtacc agagattgac cctagtcaaa
    27181 ttgatagtcg ggtacaaatt gaaaatgtca tggtgtcaga taaagaagtg tcgattacga
    27241 gtgaccaaga gacattggat agaattgata agattatcgc tgttttgcca actagcgaac
    27301 gtataacagg taattacagt ggttcagtac ctttgcaggc aatcgaccgc aatggtgttg
    27361 tcttaccggc agttatcact ccgtttgata caataatgaa ggtgactaca aaaccagtag
    27421 caccaagttc aagcacatca aattcaagta caagcagttc atcggagaca tcttcgtcaa
    27481 cgaaagcaac tagttcaaaa acgaattaaa aatagaaaag gattttataa caatgggtaa
    27541 atattttggg actgatggag tccgtggaga agctaaccta gaactaacac cagaattagc
    27601 ctttaaacta ggacgttttg gaggctatgt tcttagtcaa catgaaacgg aagcgccgaa
    27661 agtctttgta ggacgtgaca cacgtatttc aggggaaatg ttggaatcgg ccttggtggc
    27721 aggtctcctt tcagtaggga ttcacgtata caaacttggt gtccttgcaa caccagcagt
    27781 agcttacttg gttgaaactg aaggagcaag tgccggtgtc atgatttctg ctagccacaa
    27841 cccagccctt gataacggaa tcaagttctt tggcggtgat ggcttcaaac tagatgatga
    27901 aaaagaagca gaaattgaag ccttgctaga tgctgaggaa gacactcttc ctcgtccaag
    27961 tgcagaaggc ttaggaattt tggtagatta tccagaaggc ttgcgtaagt atgaaggata
    28021 ccttgtttca actggaactc ctcttgatgg aatgaaggtt gccttggata cagctaatgg
    28081 agcagcttct accagtgccc gtcaaatctt tgcagacctt ggtgcccaat tgacggttat
    28141 cggggaaaca ccagacggtc ttaacatcaa ccttaatgtt ggttcaacac atccagaagc
    28201 ccttcaagaa gtggtcaaag aaagtgggtc agctattggt ttggcctttg atggagacag
    28261 tgaccgcttg attgctgttg atgagaatgg tgacatcgtt gatggtgaca agattatgta
    28321 catcatcgga aaataccttt ctgaaaaagg acaattggct caaaatacaa ttgtgacaac
    28381 tgttatgtct aaccttggtt tccacaaggc cttgaatcgc gaaggtatta acaaggcagt
    28441 tactgcagtt ggtgaccgct acgttgttga agaaatgaga aaatcaggct acaaccttgg
    28501 tggtgaacag tctggtcacg ttatcttgat ggattacaat accacaggtg atggtcaatt
    28561 atcagcagtt caattgacta aaatcatgaa ggaaactggt aagagcttat cagagttggc
    28621 ggcagaagta acgatttatc cacaaaaatt agttaatatc cgagtggaaa acgtcatgaa
    28681 ggaaaaggcc atggaagtgc cagctatcaa ggccatcatc gagaagatgg aagaagaaat
    28741 ggcggggaac ggccgtatcc ttgttcgtcc aagtggaaca gaacccctct tgcgtgttat
    28801 ggcagaagcg cctacaacag aagaagtaaa ctactatgtt gataccatca cagatgtagt
    28861 tcgtgctgaa attgggattg actaaatcct agaaaactag atgaaaataa aaaattatgg
    28921 tacaatagct tataatattg tagccgatgg agaaagacat gaatcttaaa cgtgaacaag
    28981 aatttgttag tcagtatcat tttgatgcgc gtaattttga atgggaaaat gaaaatggag
    29041 ctcctgaaac caaggtggat gtgaatttcc aattgctcca acatgaccaa gaaaatcaag
    29101 tgacttcctt gattgtcatc ttgagcttta tgattgtatt tgataagttt gtcattagtg
    29161 gaacaatctc acaagtaaac catatcgatg gccgtattgt caatgaacca agtgaattga
    29221 accaagaaga ggtggaaacc ttggctcgtc catgtttgaa catgctcaat cgtttgactt
    29281 acgaagtgac tgaaattgca ttagacttac caggaattaa tttggagttc taatatgaaa
    29341 ttagctgttt tcacagattc ctctgcttat ctcagtgcag agatcttgca aagagaagac
    29401 ttgtttgtct tggatattcc tgtcaatatt gatggtgagg aatatgtcga aggcatcaat
    29461 ctgtctgctg aggaatttta ccaaaaaatg gctcaggctt ctgaattgcc taagaccagt
    29521 caaccaagta ttgccaagtt agatgaaatc ttaacttcgc tcaaagaaca aggctataca
    29581 catgccttgg ggcttttcct atcttctgga atttcaggtt tttaccaaaa tatccagtat
    29641 atggtcgatg actatgaggg cttaaccatt gctttcccag acactttgat tacaagtgct
    29701 cccctaggca tcatggttga aagcgtcttt aattggcgtg atcagggcga tgattttgcc
    29761 agcattcagg ataagctagc cattcaaatc agccgtacgt cagcctttat catggtagat
    29821 gacttggatc atttggtaaa aggtggacgc ctttcaaatg gggctgccat tttgggcaat
    29881 ttgcttagca ttaagccaat cctttatttt aacgatcaag gtgtgattga agtttacgaa
    29941 aaagttcgta ctgaaaagaa ggccaccaaa cgcttaattg aaattatcaa ggaaacaacg
    30001 gcttcaggtc aataccgggt cattgtcatt catgggaatg ctcctgaaaa ggctgaagaa
    30061 ttgcgtcagc atttgcttga ttttggcttg ggttcggatg tttcacttgc tacatttggt
    30121 agtgtcattg gaacgcacct aggagcagga agtattgctc taggttatat tccagtgatt
    30181 tagttggcac ttctaggcta gttaatctta gaaattgaac acggactacc tgcctcttga
    30241 aaaaagatgc ctgtcttgcc tttcgtggaa agtcagtgcc attccctatt tttcaggggc
    30301 agctaaggtc ctttgtatct tgataattga acacgcctgg aaccctgtgt gaaaaagata
    30361 gttcttccaa gaagtagaca cttcttgatc agaattccta ttttcacttt gtgttcttac
    30421 aggctttgta ttttggacag gagtagagat gagtattcga gtaattattg ccggttttaa
    30481 gggaaagatg ggccaggctg cttgtcagat ggtattgact gatccagact tggacttggt
    30541 agcagttttg gatccttttg agtctgagtc agaatggcag ggtattcctg ttttcaagga
    30601 taaggctgat ttagctggtt ttgaagcgga tgtctggata gattttacta ctccagctgt
    30661 tgcctacgaa aatacacgtt ttgctcttga aaatggcttt gctccagtag ttggaacgac
    30721 tggtttcacg agtgaagaaa ttgcagagct aaaagaattt tctcgtgccc aagacttggg
    30781 tggcctgatt gcccctaact ttgccttggg tgctgtctta ctcatgcaat ttgcgacgca
    30841 ggctgccaaa tatttcccaa atgtggagat tattgagctc catcatgaca agaaaaagga
    30901 tgctccgagt ggaacagcca ttaaaacagc tgagttgatg gcagaggttc gagagtcaat
    30961 tcagcaaggt gcagcagatg aggaagagct gattgctggt gctcgtggtg ctgactttga
    31021 tggtatgcgc atccactcag ttcgtttgcc aggcttggta gctcatcagg aagtcatctt
    31081 tggcaatcag ggagaagggt tgaccctccg tcatgactcc tatgatcgca tctccttcat
    31141 gacaggagtc aatttgggaa ttaaagaagt tgtcaagcgt catgagcttg tctatggatt
    31201 agaacactta ttatgagatt aacgcaaatg ccttctgaat ttcagaaggc tttaccagta
    31261 ttagaaaaaa ttaaagaagc aggctttgag gcttattttg ttgggggctc tgttcgagat
    31321 gcccttctcc atagccctat ccacgatgtg gatattgcga cgtcttctta tccagaagag
    31381 accaagcaga tttttccgcg aacagccgat atcggaatcg agcatggaac cgtcttggtc
    31441 ttagatgggg atgaggagta tgaggtaaca acctttcgga cagaggatgt ctatgtggac
    31501 tatcgcagac ccagtgcggt ttcctttgtg cgctcgctag aagaagacct caaacgccgt
    31561 gatttcacag tcaacgcctt tgccttggat gagacaggag aaatcgttga cttgttccat
    31621 ggtttagaag atttggaaaa gcaagtcttg cgagcagttg gagtggctag tgagcgtttc
    31681 aacgaagatg ctttacggat tatgcgtggt ttccgttttc aggctagtct tggttttgca
    31741 cttgagccag aaacatttaa agctatgaag accttgacgc cgcttttgga gaaaatttct
    31801 gtagagcgta ccttcgtcga atttgataaa ctcttgctgg ctccattttg gagaaggggc
    31861 ttggcttcca tgattgagag tcaagcttat gactatctcc ctgatatggc atctagccag
    31921 gacaagctca acagactgtt tgatttggag actgatttta cttttgaatc ctctgaacaa
    31981 gcctgggcgg ctttactatg ggctttggag attgaaaatg cgcagtcatt tttgaaatct
    32041 tggaagacct cacgccagtt tgccaagcaa gttcaggatt tgctgattat tttggctctg
    32101 cgtgaaaatg gagaattgag caagcgagat tgttatcgct ttgacataga tttgctttta
    32161 caggctgaaa atcttcgtca agctcaagga aaagaagtca acccacaagc catcacagaa
    32221 aaataccaaa gcttgactat ccatgacaag aaagagattc agattaatgg cgatattttg
    32281 atcaaggaat atggctatca gccaggccca gacttgggag agattttaac agagattgag
    32341 tttgccattg tcgatggaga attggagaat aatcgtgaag ccatccatgc ttacctgagg
    32401 gagaaaaaat gagtgatttt atcgttgaaa aactaagtaa atccgttggt gacaagaccg
    32461 tttttaggga tatttccttt attatccatg acttagacag aattggttta atcggtgtca
    32521 atgggactgg caagaccacc cttttggacg tcctttctgg tgtttctgga tttgatgggg
    32581 atgtcagtcc tttttcagct aaaagtgatt accagattgg ttatttgact caggatcctg
    32641 attttgatga tagaaagaca gttttggata cggttctatc tagtgaactc aaggaaatcc
    32701 agctcattcg tgagtatgaa ttgattatgc tcgactatag tgaggacaag caggcgcgtt
    32761 tggaacgtgt catggcagag atggactctc tccaagcttg ggaaatcgag agtcaggtca
    32821 agaccgttct taacaaattg ggcattcaag acttatctac tcctgttggg gaattgtcag
    32881 gtggtctgag aagacgggta cagttggcac aagtcttact tggcaaccac gaccttttgc
    32941 ttttggatga gccgaccaac catctggata ttgcgattat tgagtggctg accctctttt
    33001 tgaaaaattc taagaagacc gtccttttta tcactcacga tcgttatttc ttagacgctt
    33061 tgtcaacacg gattttcgag ttggagcgag caggcttgac agaatatcag ggcaattacc
    33121 aggactatgt tcgccttaag gcagaacagg acgagcgcga tgcagctcta cttcataaaa
    33181 aagaacaact ctacaaacaa gaactggcct ggatgagcag acaaccgcag gcgcgtgcga
    33241 ccaagcaaca agctcgtatc aatcgtttcc atgatctgaa aaaggaagtt tcaggcagta
    33301 gtgctgagac agacttgact atgaactttg aaaccagtcg gattgggaag aaagtcatcg
    33361 agtttcagga tgtttccttt gcctatgaaa ataagcccat tttgcaagat tttaatctct
    33421 tagttcaggc taaagaccgt attggaattg ttggggacaa tggtgttgga aaatcaaccc
    33481 tacttaacct gattgcagga agtcttgagc cgacagcagg acaagttgtg attggggaaa
    33541 ctgttcgcat cgcctatttc tctcaacaaa ttgagggttt ggatgaaagc aagcgtgtga
    33601 tcaattacct gcaggaagtg gcagaggagg tcaagaccag tggtggttct acgacttcca
    33661 tcgctgagtt gctggagcaa ttcctcttcc cacgttcgac gcatgggacc ttgattgaga
    33721 agttgtcagg gggtgagaaa aaacgtcttt atctcctcaa actgcttttg gaaaaaccaa
    33781 atgttcttct tttagacgag ccaaccaatg acctagatat tgcaactttg acagtcttag
    33841 agaatttctt gcaaggtttt gcaggtcccg ttttaacagt cagtcacgac cgctatttct
    33901 tggataaggt agcgaccaag attctcgctt ttgaggatgg caagattcgt cctttctttg
    33961 gtcattacac cgactatctt gatgaaaaag cttttgaaac agatatggcc aatcaagtgc
    34021 aaaaggccga aaaggaaaaa gtggtcaagg ttcgagaaga caagaaacgc atgacctacc
    34081 aagaaaagca ggagtgggca agtattgaag gtgatattga aaccttggaa aaacgtatcg
    34141 ctgctattga agaggaaatg caggctaacg gctctgactt tggtaagctg gctactctcc
    34201 aaaaagaatt ggatgagaaa aatgaagcac tccttgaaaa atacgaacgc tatgagtatc
    34261 tcagtgaatt tgatagttaa gactattcga aaatcaaagt gcaaactatg tcggcttcac
    34321 attgccgact caagtacagc ctgcgaccgg cttcctaatt tgcactttga tttttattga
    34381 gtataaacga atagaaaaat ctcgtctcat gaaaggattt ctctattctt ttttggtttc
    34441 ttgatgaaag atattttgcc aggtttcgtg gcgacgccag atactggtat ggatgatacc
    34501 atctaactca tagcagatga gtttggtttt ttgactgatg gaagtaattt gaatgtcctt
    34561 gatagcagaa tttaactctt ttttggtctt ataagcctct ttatccatct gctctccatc
    34621 ttggcgaata tagacaaaat catcagtcaa gagttcttct agttggtttc cttggtcaat
    34681 caattgttca cgcataagca attttttata gacattgtct aaaatttcat cctcaaggat
    34741 aggtgctggt tcgatatgga catcggtatc aaagacgcca aaacgctcct ccagcataga
    34801 ctcgacctga tccgcgattt catggctttc aaaaacagac aagtcaggat tcatctctag
    34861 tgtaatatcc aggtagatgt tgctaccgta ggtgcgacct ctttgcgatt tgaccttgct
    34921 gattttggga atttccatga tagccttttg gtagtcctcg agcaggcggt cgtcaaagcc
    34981 atctgaaaga ctaaaggaag actcgatgaa gatatcatag gcagtcttca agataaagaa
    35041 agtgatgatg atagcaacca gtttatccac aatcggataa ttgaaactac tagctaggat
    35101 ggcaatggcg gttccaagtg aggtaacagc gtcagaaaga ttgtccttag cagctgcctt
    35161 cagcgccttg gagttggatt tcttactgag gcgagtattg tagagataga ccacaaacat
    35221 aatcgctgca gaaatgattc ctagagttgc accaagagga tcaatgaccg tttcttcccg
    35281 actgagaatc ttttgaatgg tatctcttag aacatcgaaa ccgacataga acatgatgat
    35341 agaagtgatc aagcttgcca aatcttcaat cttccaatga ccaaaacggt ggtcacggtc
    35401 tgcaggctgg cgcgccatcc gaatcccgat taagagggct acatttccaa tgatgtccga
    35461 tacgttatta aaaccatcgg ccaccaaact ggatgaatga aggagatgac cagctgctaa
    35521 tttggctgca gacaagatca aataggtcga aatactgata atggctccac gctcagctaa
    35581 cttgagattt gagatagatt gcttcattca acttcctttc tagtcacata gcattctacc
    35641 attatactgg ttttcaagga aaaattcaaa taaggcagtt tttactcttt gaaaactccc
    35701 caaaatttgg tataatggta ctactcaagg gagtagctgg cagaaacctg tgatagtgtc
    35761 gtcattccga attttatgct gaaaagtatg ctttccggcc ctatcttaaa cagcgagact
    35821 tgttatgatt aacaggtctc tttttgtttg tcgtgaaaag atatgacaga aagagagtct
    35881 gttttgcaca caatgtcaag gaggagacac atgtcaaaag aacaaaaacg ccaagcgttt
    35941 tatactcaga gccctgaaga ggtcttgcag gctatggatg cgaccgagca aggtttgtca
    36001 tcaagtgagg cggaaaagcg ccttgccgaa tttgggcaca atgaactcga agaaggcgag
    36061 aaacgatcaa tcttggtcaa attcatcgag caatttaagg atttgatgat tatcatccta
    36121 gttgcggcag caatcttgtc agtcgtgact tctggtgggg aagatatcgc agatgccatt
    36181 atcatcctag ctgtggttat catcaacgct gcctttggtg tttaccaaga aggaaaagct
    36241 gaagaagcta ttgaagccct caaatccatg tctagtccag ttgcccgcgt tcttcgtgat
    36301 ggacacatgg cagagattga ctctaaagaa ttggtaccag gcgatatcgt tgcccttgaa
    36361 gcaggtgacg tggtaccagc ggacctacgt ttgatagaag ccaactctct taaaattgaa
    36421 gaagcagcct tgacaggtga atctgtacca gtcgaaaaag acttgtcagt cgagcttgcg
    36481 acagatgctg gtattggtga ccgtgtcaac atggccttcc aaaactcaaa cgtaacctat
    36541 ggtcgtggga tgggtgttgt tgtcaataca ggtatgtata ctgaagttgg tcatattgct
    36601 ggtatgcttc aagatgcgga tgagactgat acaccactca aacaaaactt gaacaacctt
    36661 tctaaggtct tgacctatgc tatcttggtc attgcccttg ttacttttgt agtgggtgtc
    36721 ttcattcaag ggaaaaatct acttggtgag ttgttgactt ctgttgccct tgccgttgca
    36781 gccattccag aaggacttcc tgctatcgtt accatcgttc tttctcttgg tactcaagtt
    36841 ttggccaaac gacattccat cgttcgtaag ttgccagcag ttgaaacact tggttcaact
    36901 gaaatcatcg cttctgataa gactggtacg ctgactatga acaagatgac agtcgaaaaa
    36961 gtcttctacg atgcggttct acatgactca gctgatgata ttgaactagg tcttgaaatg
    37021 ccactacttc gttcagttgt cttggccaat gatacgaaaa tcgatgtgga aggtaacttg
    37081 attggtgacc caaccgaaac agcctttatc caatatgcct tggacaaggg ctatgatgtc
    37141 aaaggtttct tagagaaata tcctcgtgta gctgaattgc catttgcctc tgaccgtaag
    37201 ctcatgtcaa cagttcaccc attgccagat ggtcgtttcc ttgtagcagt caagggtgcg
    37261 ccagaccaac tcttaaaacg ttgtcttctt cgtgataagg ctggggatat tgctccgatt
    37321 gatgagaagg ttacaaatct cattcataca aacaattctg aaatggctca tcaagccttg
    37381 cgtgtccttg caggtgctta taagattatc gatagtattc cagaaaatct cacttctgaa
    37441 gagcttgaaa atgatttaat ttttactggt ttgattggga tgattgaccc tgaacgtcct
    37501 gaagccgctg aggctgttcg tgtggctaag gaagcgggaa tccgtccgat tatgattaca
    37561 ggtgaccacc aagacacagc ggaagccatt gccaaacgtt tgggaatcat tgacgcaaac
    37621 gatacagaag gtcacgtttt aactggtgct gaactcaatg aactgtcaga tgaagaattt
    37681 gaaaaagtcg ttggtcaata ctctgtttat gcccgtgtgt ctccagaaca caaggttcgt
    37741 atcgtcaagg cttggcaaaa acaaggtaaa gtcgttgcca tgacaggtga cggtgtcaat
    37801 gacgcgccag ctctgaaaac agccgatatc ggtatcggta tgggaatcac tggtacagag
    37861 gtttctaagg gggcttctga tatgattctt gcagatgata actttgcgac tattatcgtc
    37921 gcagtggaag aaggacgtaa ggtcttctca aacattcaaa agactattca gtacctactt
    37981 tctgctaata ctgctgaagt attaaccatc ttcctatcaa ccttgtttgg ttgggatgtc
    38041 ttgcagccgg ttcatctttt gtggatcaac ttggtaacgg ataccttccc agctatcgct
    38101 cttggtgttg aacctgcgga gcctggtgtc atgaatcata aaccacgtgg acgcaaggca
    38161 agcttcttct caggtggtgt tttgagttct atcatttatc aaggtgtact ccaagcagct
    38221 cttgttatga gtgtttatgg ccttgcgatt gcttacccag ttcatgtggg tgacaatcat
    38281 gctattcatg cagatgccct aacgatggcc tttgcaaccc ttggtttgat tcagctcttc
    38341 catgcctaca atgtcaagtc tgtctaccaa tccatcttga cagttggccc attcaagtct
    38401 aagaccttta actggtccat cttggtatcc tttatccttc tcatggcaac aatcgttgta
    38461 gaaccgcttg aaggaatctt ccacgtaacc aaactagact tgtcacaatg gggaattgtt
    38521 atggctggaa gcttctcaat gattatcatc gtcgaaatcg ttaagtttat tcaacgcaaa
    38581 ctcggttttg acaagaatgc gatttaaggg aggtttcgga tgtcagcata tcaattaccg
    38641 accgtatggc aggatgaagc tagtaatcaa ggagctttta cggggctaaa cagaccaaca
    38701 gcaggtgccc gtttcgaaca aaacttgcca aaaggagaac aagcttttca gctttattca
    38761 ctgggaacac caaatggtgt gaaggttact atcttattgg aagaattact agaagctggt
    38821 tttaaggaag cggcttacga cttgtataag attgctatca tggatgggga tcaattcggc
    38881 tcagactttg tgaagctcaa tccaaattcc aagattccag ccttattgga ccagtcaggt
    38941 actgaaaacg taagagtctt tgagtctgct catattcttc tttaccttgc tgagaaattt
    39001 ggagcctttt taccaagtaa tcctgtggaa aaggtagaag ttttgaattg gctattctgg
    39061 caagcaggtg cagcaccttt tctaggtggg ggatttggac atttcttcaa ttatgctcct
    39121 gaaaaattgg aatatcctat taaccgtttt acgatggaag tgaaacgcca gttggattta
    39181 ttggataagg aattggctca gaaaccttat attgcaggca atgactatac gattgcagat
    39241 attgctatct ggtcttggta tggacagtta gttcaaggaa atctttacca aggttctgca
    39301 aaattcttgg atgcctcaag ttatcaaaat ctagtaaaat gggcagaaaa agttgccaat
    39361 cgtccagctg ttaagcgtgg cttggaagta acttatacag aaattaaata gcaaaaaagt
    39421 catcttcgga tggctttttg ctacagttga gggaaagggt ttgcagttat cggcttttgt
    39481 gctataattg ctataatata gtaaaaagca agaggagtgt gaggaagtgg aaaagaaaat
    39541 tgtgaaggat atcttatttt tatctcaagt gtctcagccg gcaagtcagg aggaccttta
    39601 tcttgccaga gatttgcagg atacactctt agcaaatcgt gatacctgtg ttggtctagc
    39661 tgccaatatg attggggtgc agaagcgcgt gattatcttt aatcttggct tggttcctgt
    39721 ggtcatgttt aacccagtgc ttctgtcctt tgaaggatcc tatgaggcag aagaaggctg
    39781 tttgtccttg gtaggtgtga gatcaactaa gcgttatgaa accataaggc ttgcctatcg
    39841 tgacagcaag tggcaggaac agaccattac cttgacaggc ttcccagctc agatttgcca
    39901 gcatgagctg gatcacttgg aaggacgaat catttaggag gaaagcaaat gaaacgaata
    39961 gtctttgaac ttatttttat cgcaacgacc tggtatatct ttttaccgcc ccttaacctg
    40021 accagctggg aatttctctt cttcctctgt gggcatttgt tagttgtggc aatattattt
    40081 ggctttggca aggggataaa ccttgtcaaa acggttcatg tgcgccacgg taaggcggaa
    40141 gctgccttaa atcttgaggg tttcaaaatc aatcggttag ggaaaattct gttagcttcg
    40201 attggaggaa ttcttctctt ggcagctttg gtttccttgg taacttccag catgtttcag
    40261 gctaaaaatt atgccaatgt agtcacggtt acggaaaaag actttactga atttcctaag
    40321 agtgacacca gtaaggttcc tatcctagat agaagtactg ctgaaaaaat tggagaccgc
    40381 tacttgggtt ccctaaccga taaggtgtcg caatacgtag cggcagatac ctatacccaa
    40441 ttgacaattg atgggaaacc ttatcgggtc acaccactag aatatgcaga ccctatcaaa
    40501 tggtttaaca atcaagccaa gggaatcggt gagtatatca aggtggacat ggtaactgga
    40561 aatgcggatt tggtggactt gaagacacca atcaagtatt cagactcgga gtattttaac
    40621 cgtgatgtca aacgtcacct gcgcttgaag tacccgacca aaatctttaa aactccatct
    40681 tttgaggtgg acgatgaggg caatcctttc tatgtagcaa cggtttacca aaagcaattt
    40741 ggacttgcgg ttcctcgtcc tgcttcagtc attatcttgg atgctacaaa tggagaaacc
    40801 aaggaataca gcttatcaga tgttccagaa tgggtggaca ggatctatcc agcagaggaa
    40861 accattgagc aaattaacta caacggcaag tacaaggacg gtttcttgaa tgccatgatt
    40921 tccaagaaaa acgtgaccca gactaccaag ggctataatt acttgtctat cggtaatgac
    40981 atctatctct acacaggtgt gacgtcggct aatgcggatg agagtaatct tggtttcatc
    41041 cttgaaaata tgcgaacagg agaaatcact aagtatagct tggcttctgc gacagaagaa
    41101 tcagcccgtg aatcagcaga aggtgctgtt caggagaaat cctacaaagc aaccttccca
    41161 atcctcatca acctcaatga caagcctctc tacatcatgg gcttaaagga caatgctggc
    41221 ttggtcaaag agtacgccct ggtagacgca gtcgagtacc aaaatgttat cgttgctact
    41281 acagtggaag agatgctcag caagtatgcc aataaaaacg accttgaaat tgacaatgca
    41341 acgacagaaa gcatcaaggg agtagtagca gacctcaaat cagctgttat caagggagac
    41401 actgtctact tctttaaagt tgatggcaag atatacaagg tcaaggcttc agtatccgat
    41461 gaccttcctt accttgaaaa tggtaaaacc ttcgaaggtc aagtaggaaa agacaattat
    41521 ctcaagacct ttaaagtaca ataatactct tcgaaaatct ctagctctat ctgcaacctc
    41581 aaaacagtgt tttgagcagc ctgcggctag tttcctagtt tgctctttga ttttcattga
    41641 gtataaaaaa ggtttattta ataaatatat gttataataa ggtaaattaa gcctaatgga
    41701 ggacaaagaa atgggttttt atttgatggt tgcttcaatg ctactcggac tgctcgcttt
    41761 gaagataggt ttttctcagt tcaaggagaa aaaagataaa ttcttatcta tcttaacaag
    41821 tttagctggc acagcccttg ttctcgtggc tgtctggtta ggatggccca aataaataga
    41881 aaaacctcgg tcaaaccgag gtttttcaaa tgaatttctt ggttgtggct aaaaaatatg
    41941 ctacactatc aatatgaaaa ttttaatccc aacagcaaaa gaaatgaaca cagacttgcc
    42001 aagtatcgag gcaattcctt taaaaccaga aagtcagacc gtgcttgatg ccttggctct
    42061 ctattctgcc agtcaattgg agagtttcta caaggtatcg gctgagaaag cggcggaaga
    42121 atttcaaaat atccaagctt tgaaaaggca aactgctcaa cactatccag ccttgaaact
    42181 ttttgatggg cttatgtacc gcaacattaa gagagataag ctgaccgagg cggaacaaga
    42241 ttatcttgaa aatcatgtct tcattacctc ggctttgtac ggtgttgttc cagccttgtc
    42301 acccatggct cctcaccgtt tggatttttt gatgaaatta aaagtcgctg gtaagacttt
    42361 gaagagccat tggaaggcag tctatgatga agctctgaag aaggaagaag tgattttctc
    42421 tctcttgtca tcagagtttg agactgtatt ttctaaggaa atcagagcaa agatggtgac
    42481 cttcaaattc atggaggata gaggcggtca gctgaagatt cactcaacta tctccaagaa
    42541 agcgcgcggg gcctttctaa cagctttaat agaaaatcaa gtacaaactg tgggggaagc
    42601 acgtcgcttg aactttgctg gatttgttta ccgagaagat ttgtcacaac cacagggatt
    42661 ggtttttgta aaggaagtat agaaatcaga tattttgtat ttggcaatat gtatcatcag
    42721 ctgttttagt ttataaatga gaaaaaaagc gaacaagcta gtcttctgag tgtcagaaaa
    42781 gtagtcttgt tcgcttttat tatgttatac tcttcgaaaa tctcttcaag ccacgtcagc
    42841 gtcgccttac cgtatatatg tcactgactt cgtcagttct atctacaacc tcaaagcagt
    42901 gctttgagca gcttgcggct agcttcctag tttgcttttt gatttttatt gagtgttagt
    42961 tacttgatag cttcgaccaa gtcttgagct tgtttttcaa gtgagtttag gactgtttct
    43021 tcaagaacca attttccgtc tgcccaggca gagtcattaa cacgtgcagc agtgaaatca
    43081 ccaacgcctt gtgtacggat aaatggcaag aggtctttgt agatagcgaa aagttgatcg
    43141 tgccctgcat tggctacaga tgatactgtg acaaacttgt cttgaagggc agaaacgcca
    43201 cgtgtatcag acaagtcaag ggcacgagat agccagtcaa gcaagttttt cactgtacca
    43261 gggatagaga agttgtagac tggagagaaa atccagatag catccgcaac gagaactgct
    43321 tcacgagcag cagctacagc tggatgtgtt ggaacttcca aatcttggct gaagagagga
    43381 agggctgaat aatcaaggta gctaacttcc gctttaccag caagtgcttt ctcagcttcg
    43441 agcgccattt ggtggttgaa agaaccttga cgtagtgatc cgacgataaa taatactttt
    43501 ttagacatga tgtgtctcct ttttcttaaa atttcgagaa ctctctcgtt gttataaaat
    43561 atattacaac aaaacaaaag aacttgcaag aaatttgctc agagtttatt tgaaaattgt
    43621 aaaatttcgg aaactctagt catttgtgac tgaaaaagta agaactgttt cactttcaaa
    43681 atcggttttg tttgaaggga gaaatagaat tggttgctca aagcacagct ttgaggttgc
    43741 agataaagct gacgtggttt gaagagattt tcgaagagta ttcttatatt cacttgtaat
    43801 aaagcttaat agatggtaaa atagacgagt gaaaaaaaga caaaacaaag caaaaaataa
    43861 tctactgtgg cagtatggtc tagggatgac gattttgttt gtggttatca gtgcttcctt
    43921 tctgtatatg gtttctctta gcatgaaacc ctatcaaaca gctaaaagtg aaggagaaaa
    43981 attagctcag cagtatgcag gattagagca ggctgatcag gttgatttat acaatggctt
    44041 ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa gaagcgcttg ctgtcctgat
    44101 tggtaaagat gaccataaga tttacgttta tcagctaaat cagggtattt cacaagaaaa
    44161 agcagaaacg gtttctaagg aaaagggagc tggcgagatt gacaagataa cctttggtcg
    44221 ttatcaagac aagccaatct gggaagttaa gtcaggatct gatttttatc tagtagattt
    44281 tgaaacagga gcattggtca acaaggaggg cctatgaaat tatccaaccg tgttttagaa
    44341 atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca aagcactgaa ggctgagggt
    44401 agagatattt tgtctctaac cttgggtgag ccagatttca ctactcccaa aaatatccaa
    44461 gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt tttatacagt aacctcaggt
    44521 ctgccagagc ttaaggcggt ggtcaatagc tactttgagc gcttttacgg ctattctgta
    44581 gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt ctctctatac cttctttatg
    44641 gctgtggtca atccaggtga tgaagtcatc atcccaaccc catactgggt tagctatgga
    44701 gatcaggtca agatggcaga aggcgttccc gtctttgttt ctgctaagga agacaatcac
    44761 tttaaggtga ccgtagagca gttagaagca gcttgcactg acaagaccaa ggttttggtg
    44821 ctgaattcgc catctaatcc cacaggtatg atttacaccc gtgaggaact cttggcaatt
    44881 ggaaactggg ctgtagaaaa tgatattctc atcctagcag acgatatcta tggccgcttg
    44941 gtttataatg gtcatgagtt cacaccgatt tctagcctat cggaagcgat tcgcaagcaa
    45001 acagtggtca tcaatggtgt gtctaaaacc tatgccatga ctggttggcg gattggttat
    45061 gccgttgggg aagcagacat tatcgctgcc atgtccaaga ttgcaggtca aacaacttcg
    45121 aatccgtcag cagtagccca atatgcagca gttgaggctc tatcaggtga gcaagatact
    45181 gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata ccatctatcc cctccttgca
    45241 gaagtgccag gatttgaagt ggtcaaaccg caaggggcct tctatctctt cccaaatgtc
    45301 aaaaaggcca tggagatgaa aggctacacg gatgtgacag actttacaac tgttatctta
    45361 gaagaagccg aagtggcctt ggtaacagga gctggatttg gagcaccaga aaatgtccgc
    45421 ctcagctatg cgacagacct agacacgctt aaagaagcag tcgaacgctt gaaagcattt
    45481 atgggtagtg agaatgattg atcattttga gattaaggta aaggatttac aaatttcagg
    45541 aggattttat aggagttttc tcgctccttt ggactataaa ttggctttta agactagttc
    45601 tctaattagt tttctatccc cgaacagccc tcatcctggt ggtgattttt ggctgactca
    45661 aggaacacaa gatcctgttc attttgcttt tttagcagaa aataaggaag aagtttaggc
    45721 ttgttatgag gctggcttag aggcaggtgg gcgagacaat ggggctcctg gttatcgaag
    45781 tgagcgtccg atttactatg ctgcttttat gattgacctg gatgggaaca atatagaggt
    45841 ggtttgccat aaagaataaa aaagaaaagg aaaaataatg acaaaacgtg taacgattat
    45901 tgacgtaaaa gactatgttg gtcaggaagt gacgattggc gcttgggttg ccaacaaatc
    45961 aggaaaaggg aaaatcgctt tcttacaatt gcgtgatgga acagccttct ttcaaggtgt
    46021 ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa gtgggacttg agaagtttga
    46081 tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg acaggtattg tcaaagagga
    46141 cgaacgttct aaatttggct atgagttgga catcacagac atcgaagtga tcggtgaatc
    46201 tcaagactac ccaatcacac caaaagaaca cggaacagac tttttgatgg ataaccgtca
    46261 cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa atccgtaacg ctattatcta
    46321 tgcaacttat gagttctttg acaagaacgg ttttatgaag tttgacagcc caattctttc
    46381 aggaaatgcg gcagaagatt ctacagaact ctttgaaact gactacttcg gaacgccagc
    46441 ctacttgagc caatcaggtc agctttacct agaagcaggg gctatggctc ttggtcgtgt
    46501 ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa acacgccgtc acttgactga
    46561 gttctggatg atggatgctg agtactcata cttgacacat gatgagtcgc ttgacttgca
    46621 agaagcttat gtgaaagctc ttctacaagg tgttcttgac cgcgcgcctc aagccttgga
    46681 aaccttggaa cgtgatacag aactcttgaa acgctacatt gcagagccat tcaaacgtat
    46741 cacttacgat caagccattg acctcttgca agagcatgaa aatgatgaag atgctgacta
    46801 cgagcatctt gagcatggtg atgactttgg gtcaccacac gaaacttgga tttcaaacca
    46861 ctttggtgtg ccaacatttg tcatgaacta tccagcagcc atcaaggcct tctacatgaa
    46921 accagttcct ggaaatccag agcgcgtgct ttgtgcagac ttgcttgctc cagaaggcta
    46981 tggagaaatt atcggtgggt ctatgcgtga ggaagattac gatgcccttg tcgctaagat
    47041 ggatgaactt ggcatggatc gtacagaata tgaattctac cttgaccttc gtaaatacgg
    47101 tacagttcca cacggaggat ttggtatcgg tatcgaacgt atggtaacct tcgcagcagg
    47161 aacaaaacat atccgtgaag ctattccatt cccacgtatg ttgcaccgta tcaaaccata
    47221 agaacaggaa aacgcccatg tggcgttttt tcgaattcta gtgtttttac ttgcgtaaaa
    47281 aaattttttc tagtataata gtttattgtg agcgaacctc acttacccct tgcaaagtct
    47341 tggggtcatt agaccaaaag gaggaacata tcaatggcta aatacgaaat tctttatatc
    47401 attcgtccaa acattgaaga agaagctaaa aacgctttgg tagcacgttt tgactctatt
    47461 ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt gggaaaaacg tcgtcttgca
    47521 tacgaaatcc aagatttccg tgaaggactt taccacatcg ttaacgttga agcaaatgac
    47581 gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca acgctgacat tcttcgtcac
    47641 atgatcgtca aaattgacgc gtaagaaggt aaactatgat taacaatgtt gtacttgtag
    47701 ggcgtatgac acgtgacgct gaattgcgtt ataccccatc aaatgtagca gttgcgactt
    47761 ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg tgaacgtgag gctgatttta
    47821 tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc taactgggct aaaaaaggct
    47881 cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta cgataaccag caaggacaac
    47941 gtgtctacgt gacagaggtc gtggctgaga atttccaaat gttggaaagc cgtagtgtgc
    48001 gtgagggtca cacaggtgga gcttactctg caccaactgc aaactattca gcacctacaa
    48061 attcagtacc agacttttca cgtaatgaaa atccatttgg agcaacaaac ccattggata
    48121 tttcagatga tgatttacca ttctaatgga caactaaatt tataaaggag aaaaaacatg
    48181 gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag ttgattacat cgcagcaaac
    48241 aaaattgaat atgttgatta caaagatact gagcttctta gccgtttcgt ttcagaacgt
    48301 gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta aaaaccaacg taaagtaaca
    48361 acagctatca aacgcgctcg cgtaatggct ttgatgcctt tcgtaaacga agattaaaga
    48421 aaagacccag ttgggtctct ttttcatgag cttgaaaaca agaaagcgaa ttaagacccc
    48481 aacgggtctt ttttcacaag ctttgaaatg agagagcgag ttaggctcag tggctccctt
    48541 tttacgagtt tgaaaacaag agaacacatt aaaagcacga tggagctttt tcataaatat
    48601 gtctcttttc tcatccaaga aattaaaata aaaatgataa actttttgaa aataggggat
    48661 ttgcctactt tatgatataa tggaaagagt tagattgaaa gaggtagtga gatggacgcg
    48721 aaattaagat acaaggcaaa gaagatcaag attgtctttt ttgatattga tgatacattg
    48781 cggaattcaa agacagggtt tattccaact acaattccca ctgtttttaa acagttgcgt
    48841 gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca tttttggtgt tgttccagag
    48901 attcgtgatc tcaagcctga cttttttgta actttgaatg gggcttatat cgaagataaa
    48961 aaaggtcagg tcatttatca gcatcagatt gaaaagtcag atgttgagga gtatatctct
    49021 tgggctaagc aagaaggaat tgagtatggc ttggttggga gtcatgatgc caagttgtcg
    49081 actcgcaccg atatgatgag tgaagctatc aatccaattt atcccgactt agatgtagat
    49141 cccgatttcc atgaaaaaga agatatctat cagatgtgga cttttgaaga taagggagat
    49201 gacttgcact tgcctgacag tctctcagac aaacttcgca tggttcgttg gcatcaacat
    49261 tcgtctgata ttgtgccgat ttcaggctcc aaagcgacgg gggtggaaaa ggttgtggaa
    49321 caccttggct tgaaaccaga gaaggtcatg gtttttggag atggtctcaa cgacttggaa
    49381 ctctttgatt atgctggaat cagcgttgcc atgggaattt ctcatgataa aatcaaagaa
    49441 aaagcagatt atattacaaa aacattagaa gaagatggca tttttgctgc cttagaagta
    49501 tttggtatgg tagaaaaaga attgcatttt ccacaagtag acattgaaac agtagaaggt
    49561 cctcttgcga ctattaagac caatcacgga gacttacgta tcaagctttt ccctgaacat
    49621 gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag atggctacta tgatggtgtc
    49681 attttccacc gtattatcaa ggactttatg atccaaggtg gagacccaac tggaactggt
    49741 atgggtggcc agtcaatcta cggcgaatct tttgaggatg aattctcaga agagctttac
    49801 aatatccgtg gtgctctttc catggcaaat gctggtccaa ataccaacgg cagccagttc
    49861 tttatcgtgc aaaaccaaca cctaccttat tctaagaaag aaattactcg tggtggttgg
    49921 ccagaaccga ttgcagaaat ctatgccaat caaggtggga cacctcacct agaccgccgt
    49981 cacacggttt ttggtcagtt agctgatgaa gcatcttacg ctgtcttgga tgccattgct
    50041 gctgttgaga caggagctat ggacaagcca gttgaagatg ttgtaattga aactattgaa
    50101 atcgaggact aagatgaaaa tcggtgataa gctaaagggg cgtattacag ggattcagcc
    50161 ctacggtgcc tttgttgagt tagagacggg tgatacgggg ctgattcaca tttcagaaat
    50221 tcggacagga tttattgaaa atattcatga aaccttgaaa gtcgatgaag aggttcaagt
    50281 tcaggtagtg gatttagatg aatttacggg gaaagccagt ctttctatcc gcactttgga
    50341 ggaagaaaag taccagtttc ctagacggag acgcttttca agcgaccgct ttaactacgg
    50401 ttttgctccc tttcgacgaa tgctaccaat ctggactgga gaagccctgc accatttaaa
    50461 aaagaagaag tagtttagag ttttatattt tttgtaatgt taatatgaat ttgctataat
    50521 aggaccatgg aagaagaaca attattaaaa tcaggagagc gcattaacca gctcttttcg
    50581 acagatatca aaatcattca aaatagagag gtttttagct attcggtgga tagtgttctc
    50641 ttatcacgat ttccacgttt tcctaagaag gggttgattg tggatttctg tgctgggaat
    50701 ggagcagtgg ggctttttgc tagcactcgt actcaagcac agatattgtc tgttgagatt
    50761 caggagcgtt tggcggatat ggctgaacgc tctgtccgtt tgaatggatt agaggagcaa
    50821 atgcaggtca tctgcgatga tttgaaaaat atgcctgctc acattcaggg gagtaaggtg
    50881 gatatgattt tgtgtaatcc accctatttc aaggtggatc cttattctaa tctgaacgag
    50941 agtgaacatt atctcttggc tcgacacgaa atcacgacta atttggaaga aatctgtcgt
    51001 agtgcccaga gtattctcaa gtctaatggg cgtttggcca tggttcatcg tcctgatcga
    51061 cttctggata ttttggatac gttaaaacga cataatctag cccctaagcg cctgcagttt
    51121 gtttatccaa aaagagaaaa ggaagccaat atgcttttga ttgaggcgat caaggatggc
    51181 tcaacaagtg gctttaaggt cttacctcct ttgattgtcc acaatgatga tggctcttat
    51241 acgcccgaaa tcgaagagat ttattatgga tcataaggct tatatgtacg ttctggagtg
    51301 tcgtgatgga tcctactata taggctatac gactgatatg agaagacgcc tcgctatcca
    51361 caatagtggg aagggagcca aatatacacg agcacgcttg ccagtcaaac ttatctatgc
    51421 tcaaggtttt gccagtaagg aagaagccat gtcggctgaa gctcttctca agcgtaagaa
    51481 gaggccacag aaggaagaat ttttatctga aaatcaagat agaaatttac tcagttattt
    51541 tgaagaatcg tggggagtcc tttgactcct cttacctttg tcaaaaagcg aaaaaaatgc
    51601 tacaataaag aaaataaaca aaatgaggta ttatcatgtc caagattcta gtatttggtc
    51661 accaaaatcc agactcagat gccatcggat catctgtagc ttttgcctac cttgcaaaag
    51721 aagcttacgg tttggatacg gaagctgttg cccttggaac tccaaatgaa gaaacagcct
    51781 ttgtcttgaa ctattttggt gtggaagcac cacgtgttat cacttctgcc aaagcagagg
    51841 gggcagagca agttatcttg actgaccaca atgaattcca acaatctgta tcagatatcg
    51901 ctgaagtaga agtttacggt gttgtagacc accaccgtgt ggctaacttt gaaactgcaa
    51961 gcccacttta catgcgtttg gagccagttg gatcagcgtc ttcaatcgtt taccgtatgt
    52021 tcaaagaaca tggtgtagct gtgcctaaag agattgcagg tttgatgctt tcaggtttga
    52081 tttcagatac ccttcttttg aaatcaccaa caacacaccc aacagataaa atcattgctc
    52141 ctgaattggc tgaattggct ggtgtgaact tggaagaata tggtttggca atgttgaaag
    52201 ctggtaccaa cttggctagc aaatctgctg aagaattgat tgatatcgat gctaagactt
    52261 ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa cacagttgac atcgctgaag
    52321 ttttggaacg ccaagcagaa attgaagctg caatgcaagc tgccaacgaa tcaaacggct
    52381 actctgactt tgtcttgatg attacagata tcgtcaactc aaactcagaa atcttggctc
    52441 ttggtgccaa tatggacaag gtcgaagcgg ctttcaactt caaacttgaa aacaatcatg
    52501 ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt acctcaattg actgaaagct
    52561 ttaatgcgta agattttggg ggtcagctca aaatcggaaa gtctagtttg ccttatatcg
    52621 aaaggagttt cggctccttt tttctaggag tgaagtatgt tagaaaatgg cgatttgatt
    52681 tttgtgagag atgggtcaga catgggacag gccatccaga cttccacagg taactatagc
    52741 catgttgcca tttatttgga tgggatgatt tatcatgcta gtggacaggc tggtgttgtc
    52801 tgtcaagaac cggcagactt ctttgagtcc aatcatttat acgacctcta tgtttaccca
    52861 gaaatggata tccagtcggt gaaggaaaga gcttgcaaac atcttggagc accctacaat
    52921 gcttctttct atccagatgc agctggtttt tactgctccc agtatatagc agaaatccta
    52981 cctatttttg aaactattcc tatgaaattt ggagatgggg agcaggagat tagtgatttt
    53041 tggagggagt attacataga actaggtctg cctgttcctc tgaaccaagc tggtaccaat
    53101 cctagtcagt tggcagcatc gcctctgtta caatgtaaag aaaggaatct tcatgattca
    53161 gatttttaat tcatctcgtt tgacgagaca gccatttttg gagaattgat ccgctatctg
    53221 gatcagtatg aggatgtgat tctacgggaa attaaggctc aatttccaga tgttgcagtt
    53281 gataaactca tggaagagta tataaaggca ggcttgattc tacgtgaaaa taagcgctat
    53341 tacctcaatt ttcctacgct tgaatcactt gatagtcttg aactggatca agagattttt
    53401 gtcagagaag ctagtccggt ctatcaagcc ttgttggagc agagttttga gacggaattg
    53461 cgcaatcaaa tcaatgcagc tattttagtt gaaaagacgg actttgcgcg cattaaaatg
    53521 accctgtcca attattttta caaggtcaaa cagcagtatc ctttgacaga aaaacagcag
    53581 gagctctatg acattttagg agatgttaat cctgagtatg ccctcaagta tatgacggct
    53641 tttttgttga aatttctcaa aaaagaccag cttatgcaga aatgccgtga tatctttgtg
    53701 gacagttagg ttgtcttagg ctatattgtg caaaatgaag atggaaagta tgagttggct
    53761 atcgattttg ataaggagag gttaactttc tacttagcgt gatttcttgt ttctgagtac
    53821 attgtttgac tttccttagt attcggtata aactatatgt aaccggtaac acatatcgga
    53881 ataaactaaa ggagacaatc atatgtcact tgaaaacaaa ttggaacaag caacaggcgc
    53941 tgtcaaagaa ggttttggta aagttactgg agacagcaag acagaacttg aaggagctgt
    54001 tgaaaaaaca gtttctaagg caaaagacgt tgtagaagac gcaaaaggtg ctgtagaagg
    54061 tgccgttgaa ggtttgaaaa acgtttttac taaataatag gaaaaaatca agggtttcat
    54121 tttcccttga ttttttctat tcttataaat aattttctgc gacggctgta tctcctgggt
    54181 aggattcttt cttgccctgg atgatttggt aacaatcggc tcccttaccc gcaataataa
    54241 ctgcatctaa ttcgtgattt gtgatagcca ttgccgcctt gatggcttct tggcgatccg
    54301 caatcttctc aacaggatga ttgatgtagc tactaatttc atctgcaatg gccattgggt
    54361 cttcatagtt agggtcatca gcagtcagaa agacttgaat ctcagggtgt tgattgagga
    54421 ggaggccaaa gtccttacga cgactttctc ccttgtttcc tgttgatccc agaaccagag
    54481 caatctttcc ggtttgatga gtttcaacca cattgatgag ttttttcaga ctatccccat
    54541 tgtgggcata gtcgatgaag accttggctc catttttctg agtgaggact tccatacgac
    54601 caggaacgcg ggttgcagcg atgccttttt tgatgtcctc aagacttgct ccgagacgga
    54661 gacaagcaag tccagcagca actgcatttt cttggttgaa gttgccaatg agttggatat
    54721 cataatctcc agcgagttta cccgtagctg aaaatctaaa ggctttggaa ttctcgattt
    54781 ggttatcaaa ttggctacca tagaaatcat ggtcttgatc ttcaacctgt tctttcaaga
    54841 ctgagaagtg gtccatgtca ctgttaatga tgactgctcg gctattttcc atcaagagac
    54901 gcttgtggta gaaatagtct tcaaagctag ggtgttcaat cgggccgata tggtctaggc
    54961 tgatatttag gaaaactccc acatcaaagg ttagaccata gacacgtttg accagatagg
    55021 cttgactgga gacttccatg atgaggtggg tacggtcatt ttgcacagcc tgattcatca
    55081 tgtcaaagag gtcaatactc tcaggggttg tcaacgctga cttaaagaaa gtctctccat
    55141 caagagttgt gttcatggtc gacaacatag caggtcgatg cccttgagat aagatgttat
    55201 aggcgaaata ggctgctgtt gtcttaccct tagtaccagt aaaggcaagg agtttgagtt
    55261 tttcctgtgg attaccatag aactccatgg caatcaaact catggctttc tttatatcgt
    55321 tcacaatgat gacagggata ccgacttcgt agtccttttc agctatatac caagctaagc
    55381 cttgtgttat agcagaaaga aggtattctt ttttaaaggc agcgcctttt gcaaaaaaaa
    55441 gagtgtcttc tgttactttt cggctgtcgt agctgatgct atcaaaaaca actttgctgt
    55501 agttgtagtg gtaatgacct tggtcaataa tttcgcgaaa aaggccatct ttctttaaaa
    55561 tatctaatac agtttcaatt tttatcatac tttctattgt aaaccgaaag tcgcaaattt
    55621 acaagtgaca aggaaaagtt tataatggaa gataaggagt ttttcctagt tatcaaaatt
    55681 gaatgaggaa tctatgtcgc acgaaaacaa tcaccagcag gcccagatgt tacgggggac
    55741 tgcttggcta acggctagta actttatcag tcgcctactc ggggctgttt acattatccc
    55801 ttggtacatc tggatggggg cttatgcagc taaggcaaat ggtctcttta ccatgggtta
    55861 caatatctat gcttggttct tgttggtttc aacagcgggg attccagttg cggtggccaa
    55921 gcaagttgcc aagtataata ccatgcgaga agaagagcat agctttgccc tgattcggag
    55981 cttcttaggc tttatgacag gactaggcct ggtttttgct ttagtcttgt atgtctttgc
    56041 tccttggcta gcagacttgt ctggcgtggg caaagacttg atcccaatca tgcaaagctt
    56101 ggcttgggga gtcttgattt tcccgtctat gagtgttatc cgaggatttt tccaagggat
    56161 gaataacctc aaaccctatg ccatgagcca aattgctgag caggtcattc gtgttatctg
    56221 gatgctccta gcaaccttta tcattatgaa gctcggttca ggagattatc tagcagccgt
    56281 tacccaatca acctttgctg cctttgtcgg tatggtagcc agttttgcag tcttgattta
    56341 tttccttgcc caagaaggtt cactcaaaag agtctttgaa acaggagata agattaacag
    56401 taagcgtctc ttggttgata ccattaagga agccattcct tttatcctga cagggtctgc
    56461 catccagctc ttccagattt tggatcagct gacctttatc aatagtatga gctggtttac
    56521 caactacagc aatgaggact tggttgtcat gttttcttat ttctcagcca atcctaataa
    56581 aatcacgatg attttgattt ctgtaggggt ttcgattggg agtgttggtt tgccactttt
    56641 gacggaaaac tatgtcaagg gggacttgaa agcagcttct cgtctcgttc aggacagtct
    56701 caccctactc tttatgttct tgctaccagc aacagttgga gtggttatgg taggagaacc
    56761 tctttatacg gtcttctatg gtaagccaga tagtttggct ctgggcttat ttgtctttgc
    56821 agttttgcag tctattattt taggcttgta catggtcttg tctccaatgc ttcaggccat
    56881 gttccgcaac cgcaaggccg ttctctattt tatctatggt tctattgcca agctagtctt
    56941 gcaactacct accatcgccc tcttccacag ttatggtcct ttgatttcaa caaccattgc
    57001 tctcatcatt cctaacgtct tgatgtatcg ggatatttgt aaagtaactg gtgtcaagcg
    57061 caaggtgatt ttgaagcgaa ccattttaat cagtttgctg accctagtca tgtttctgtt
    57121 aataggaacc atccagtggc tgttaggatt tttcttccaa ccaagtggac gtttgtggag
    57181 cttcttttat gtagctcttg tcggtgccat ggggggtgga ctttatatgg ttatgagtct
    57241 gcgtacctat ttgttagata aggtaatagg aaaagcccaa gcagatcgcc tgcgagcaaa
    57301 atttaagctt tcgtaaggta gcatttataa ataaaaggaa taagttgaac aatcttttag
    57361 gagaacttga aacttattcc ttttttagcg taaaaatagc taaaattatc tttcaaaaat
    57421 agaaaatgag gtttttttcg attttttgga aaaataattg actaaacaaa ccaaacgttg
    57481 tataataaaa caaatattta aagcaggagg ttctggaaat gaaaaagtct aagagcaaat
    57541 atctaacctt ggcaggtctt gtcctgggta caggagtttt attgagcgcg tgtggaaatt
    57601 ctagcacggc gtcaaaaacc tacaactatg tttattcaag tgatccatct agcttgaact
    57661 atctagcaga aaaccgcgca gcaacatccg atattgttgc aaatttggta gacgggttat
    57721 tagaaaatga ccaatatggg aatattattc catcattagc agaggattgg actgtttctc
    57781 aggacggttt gacctatacc tacaaacttc gtaaggatgc caagtggttt acttctgagg
    57841 gagaagaata tgcgcctgta actgcccagg attttgtgac aggtttgcaa tatgcagctg
    57901 ataaaaaatc agaagccttg tatctagtgc aggactctgt tgctggtttg gatgactata
    57961 tcactggtaa aacaagcgac ttttcaactg tcggtgtcaa ggcacttgat gaccaaacgg
    58021 ttcaatatac tttggttaaa ccagaacttt actggaattc aaaaacactt gcaacgatac
    58081 tttttcctgt taatgcagat ttcctgaaat caaaagggga tgattttggg aaggcggatc
    58141 catctagtat tttgtacaat ggacctttct tgatgaaagc acttgtctca aaatctgcta
    58201 ttgaatataa gaaaaaccct aattactggg atgctaagaa tgtctttgta gacgatgtga
    58261 aattgaccta ctatgatggt agcgaccaag aatcactgga acgtaatttt acagctggtg
    58321 cttatactac ggctcgtctt tttcctaaca gctccagcta tgaagggatt aaagaaaaat
    58381 acaaaaacaa tatcatatat agtatgcaaa attcaacttc atatttcttt aattttaacc
    58441 tagataggaa gtcttacaat tatacttcta aaacaagtga cattgaaaag aaatcgactc
    58501 aggaagcagt tctcaataaa aacttccgtc aggctatcaa ttttgctttt gacagaacat
    58561 cttatggggc tcagtctgaa gggaaagaag gtgcaacaaa gattttgcgt aacctagtgg
    58621 ttcctccaaa ctttgtcagt atcaagggaa aagactttgg tgaagttgta gcctctaaga
    58681 tggtcaacta tggtaaggaa tggcaaggta tcaactttgc ggatggtcaa gacccttact
    58741 acaatcctga gaaatccaag gctaagtttg cggaagctaa gaaagaactc gaagcaaagg
    58801 gtgttcaatt cccaatccac ttggataaga ctgtggaagt aacagataaa gtaggcatac
    58861 aaggagttag ttctatcaaa caatcaattg aatctgtttt aggttctgat aatgtagtga
    58921 ttgacattca gcaattaaca tcagatgagt ttgacagttc aggctacttt gctcaaacag
    58981 ctgctcagaa agactatgat ttatatcatg gcggttgggg acctgattat caagacccgt
    59041 caacctatct cgatattttt aatactaata gtggaggagt tctacaaaat cttggactag
    59101 agcctggaga agccaatgac aaggctaagg aagttggact ggatgtctat actcaaatgt
    59161 tggaagaagc taataaagag caagatccag ccaaacgtta tgagaaatat gctgatattc
    59221 aagcttggtt gattgatagt tctttagttc ttccaagtgt ttcgcgtggg ggaacaccat
    59281 cattgagaag aaccgtacca tttgctgctg cctatggttt aaccggtaca aaagggattg
    59341 aatcatataa atacctcaaa gtacaagata agattgtcac aacagacgaa tatgcaaaag
    59401 ctagagaaaa atggttgaaa gaaaaagaag aatccaataa aaaagcccaa gaagaattgg
    59461 caaaacatgt caaataaaat ctaattgtag ataagtttgt gtaagaagtc tttgtgctta
    59521 aaaacgcata atatcaggta tataaaatcc tagatgttat gcgttttgtt ataggaagat
    59581 ttattagatt ttatcctcaa attgagttat tcccgacttt atccgttgaa atattataag
    59641 gagtatttat gaaattaaaa ttattaagag tagatactaa ggtgattatg gggagtttct
    59701 tccttgttct gtctagtcta cttgctttgt tgcttcccct tatcttaaaa ggtttaatag
    59761 atgggagttc tattgaaaat ataggttcca aagtatttca atcgtttttg atttttattg
    59821 gtcaagcctt gttttcttct attggttact atctgtttag tcaatcgggt gaaaaaaaga
    59881 tagcaaaaat caggaaaaaa gtgatagagg ggttgattta tgcagagaaa tccttctttg
    59941 ataagagcca aagtggggag ttgacttctg ccattgtcaa tgacatgagt gtcattcgtg
    60001 agtttttaat tacgactttc ccaaatatta ttctgagttt agttatggta cttggttcca
    60061 ttgtagtctt atttagtctt gattggaatc tttctctact tttattcatc actcttcctt
    60121 gtatgatgtt tattatcttg cccctttcca atatcagtga aaagtatagt cgtcgtttac
    60181 aggaggaaat cggattttta acagggcaat tgactgaaaa gattcaagaa catgaattaa
    60241 ttaagactaa ccaagcagag aaaagtgtac aggatgtttt ggataattgt attgaaaggg
    60301 tacaaaacaa ttcactgaaa tcagataggg ttacttcttt tgagaccccg tttgctctct
    60361 tatttatctt tgcgactata gctgtgatgc taacctatgg gggttatcgg gtcagcgcag
    60421 gatatatatc tgtgggaacc ttggtttcgt ttttgattta cctctttcaa ttacttaatc
    60481 ctattagtaa tatagctaat tttgtaactg tttattctag gagcaaggga tcttcagttg
    60541 cactggagaa cttgcttgca gttcctaaag aaaaatttga gggaggaaaa tcggtatcag
    60601 gataaaggtt gaattttaac catgtctatt ttggttatga tgaaaatcga cctgtcttaa
    60661 aggatattac ttgttcaatt ttcaagggga aaaaaattgc ttttgttgga ccatctggat
    60721 caggaaaatc aacgattgtg cgtttgttag agcggtttta taaaccgctt tcaggagata
    60781 ttctaatgga gcaatcaagt atatatgatt ttaacttaaa agaatggaga agtaaaatcg
    60841 cttgggtttc acaaaataat gcagtcttat ctggcagtat tcgtgacaat ctttgtctcg
    60901 gtttgaatcg cttagtaact gatgatgaat tgatgaaagt gctagactta gtatcactag
    60961 gtgatgagat tcgctccatg aaagagggac tagatactga agttggtgaa cgcggacgat
    61021 tcttgtcagg ggggcaaagc caaagacttc aaatagctag agcctactta aaagatgctg
    61081 aaattcttat atttgatgaa gccactgcta atcttgatgc ggattctgag tatgcgatta
    61141 tcagtagcct ctattctgta ttaaaggaga agacggttgt gattatagcg catagtttgt
    61201 caacggtaaa agatgtggat tgtattttct tcttagagga ggggaaaatc actggctcag
    61261 gaactcataa ggaactactg gaaaatcatg agcgttatgc tcgttttgtg caggagcaaa
    61321 tgatagagtg aagtgtcttt tgagattcac cattttatag tctattaaag ggagcaggaa
    61381 aaactccctt tttatatagt ttgaaactat aactagctct tgaaaagaag aaaatgagtt
    61441 gatgaaaata agtggtacaa tagttactat agatttggag gtattgtatg agcaaggaat
    61501 tacacattaa cacaattttg gcccaggcgg gtattaagtc agatgaagcg acaggtgcat
    61561 tggtgacacc gcttcatttt tcaacgacct atcagcatcc agagtttggt cgatctactg
    61621 ggtttgacta tacgcgcact aaaaatccaa ctcgtagtaa ggctgaggaa gtcttggcgg
    61681 ctattgagtc agcagactat gccctagcga ctagctcagg gatgtcagct attgtactgg
    61741 cctttagcgt ctttccagta ggaagtaagg tcttggcagt gcgtgatctt tacggtggtt
    61801 cttttcgctg gtttaaccaa gtggagcagg aaggtcattt ccattttacc tatgccaata
    61861 cagaagaaga gttgattgcc gagttagaaa aggatgtgga tgttctctat atcgaaaccc
    61921 caaccaatcc cttgatgttg gaatttgata tcgaaaaact agcaaaattg gctcatgcta
    61981 agggtgccaa agtggtggtg gacaatacct tctacagccc tatctaccaa cgtccgattg
    62041 aagatggagc agatatcgtt ctccattcag caaccaagta tctagcaggc cacaatgatg
    62101 tcttggctgg agtggttgtg accaatagtt tagaactata cgagaagctt ttttacaatc
    62161 tcaatacaac aggggcagtc ttgtctccat ttgacagcta ccagttgctt cgtggtctca
    62221 agactttgtc tcttcgtatg gagcgctcaa cagctaacgc ccaagaagtg gttgcctttt
    62281 tgaaggattc tccagcagtt aaggaagttc tctacactgg tcgtggaggc atgatttcct
    62341 ttaaagtagc cgatgaaaca cgcattcctc atattttgaa cagtctcaag gtcttctctt
    62401 ttgcggaaag tttgggtgga gtggaaagtc ttattactta tccaacgact caaactcatg
    62461 ctgatattcc agcagaagta cgccattctt atggtttgac agatgacctc ttgcgtttgt
    62521 ctattgggat tgaggatgct agagatttga ttgcagattt gcgccaagcc ttagaaggat
    62581 aagacaaaga tgggaaaata tgattttaca agcctgccca accgtttagg gcaccatacc
    62641 tataaatgga aagaaacaga aacggatagt gaagttctac cagcttggat agcggatatg
    62701 gactttgtgg tcttgcctga aatccgccaa gccgtgcaaa cttacgcaga ccaactggtt
    62761 tatggttata cctatgccag tgaagactta attaaggaag ttcaaaagtg ggaagctaca
    62821 caatacggtt acaactttga caaagaggct cttgtcttta tcgagggtgt ggtaccagcc
    62881 atctcaacag ctattcaaac ctttacaaaa gaaggcgagg cggttttaat taacacgcct
    62941 gtctacccac cctttgctcg cagtatcaag ttgaataatc gtagattgat tactaattcc
    63001 ttagtggaaa aggatggtct gtttgagatt gactttgacc aacttgaaaa ggatttggtg
    63061 gaagaggagg ttaaactcta tattctttgc aaccctcaca atcctggtgg acgtgtttgg
    63121 gaaaaagaag tgttggagaa gattggccaa ctctgccaaa aacacggtgt tttgttagtt
    63181 tcggatgaga ttcaccaaga tttgaccctc tttggtcaca aacaccagtc tttcaatacc
    63241 atcaatcctg ccttcaaaaa ttttgctatc gtcttgagca gtgccactaa aacatttaat
    63301 attgctggaa caaaaaattc ctatgcagtc attgaaaatc ctaagttgag actggctttc
    63361 cagaaacgcc tgttggccaa taatcagcat gaaatttcag gcttgggtta tttggcgaca
    63421 gaagctgcct atcgatacgg taaagattgg ctagaggaac tcaagcaagt ctttgaagac
    63481 cacatcaatt atgtggtgga tctatttgga aaagagacta aaatcaaggt catgaaaccg
    63541 caaggtacct acttgatttg gcttgacttt tcagcttatg acctgactga tgaaacattg
    63601 caagagttgt tgagaaatga agccaaggtt atcctcaacc gtggtttgga ttttggagag
    63661 gaaggaagtc tccatgcccg catcaatata gctatgccca aatctctgtt gcaagaagtc
    63721 tgtcagcgga ttgtggctac ttttgccaaa cgttaaaaat ccagccttct aggagaaaag
    63781 tcttcctaga aggctatttt cataggagaa aatatggtat aataaaaaga taaggtaaag
    63841 gtgaaaatat ggctaaattg attccgggga aagttcgtat cgaaggtgtt gccctttatg
    63901 aaactggtaa ggttgatatc atcaaggaaa agaacaatcg gctctacgct cgcgttgcag
    63961 aagaagaact gcgctatagt ttagaggatg atttggtttt ttgtgcctgt gatttttttc
    64021 aaaagagggg ctactgtgtg catttggcag cgctagagca ttttctgaaa aatgatgagc
    64081 gtggtcagga aatcttgtgg agtctggaag aaggtcatga agaaaaagag gctgttgaaa
    64141 ccaaggtgac cttgggtggc aagtttttgg atcgaatttt atctccaaaa tcagaatgcg
    64201 cctatgagtt atcagctgtg ggtcaggtgg aagcaggaac caatcatatc ttgtggactc
    64261 tgagaatcgg ccaaatcaat agccaaaaat actatgtcat tcgggatatt ccactttttt
    64321 taaggattgt cgagcagaga aagtcgtata tgattggcaa gatttatgag gagtctcttt
    64381 cttgggaagc ctttgatgat gccagccaag aacttttgac tttcttgcac ggactaacgg
    64441 aggaagggct ggttccagac ctctttttcc aaaaccaagg tcggcacctc tttttccctc
    64501 tgaccttttt tgagcagggt gtgaatttac tgatggcctt gccccatttt caatttgacc
    64561 atcaagtgga tagctaccaa accctgcttt ttcaggatat gcatgctgat gccaatctat
    64621 ttgcctttac agtaacagaa tactcggatt attttgaaat ggaaatcagt gagagtccta
    64681 gggttaatgt cttttatcag ggagcagtac ttttccataa aggtcaggtt tattttttaa
    64741 cagaccagca gatgcgtctt ctcaaggaaa ttaaagtgtt acctgtgggt caacaaggaa
    64801 agaaatactt gcaatttgat agcagtgatc gagacaaatt ggcctcctgt ctgactcttt
    64861 ttatccagat gggcaccgtt tcagctccag aacgtctaca aatcaaaact tttgcgccct
    64921 ctttttactt tgacagggaa aaagataatc ggattcgttt agaaattcag tttgattatg
    64981 gaaatagaca ggtatctagc cgacaagagc tagaagaatt accattttca agtgacgcgg
    65041 acttagaaga aagaattttc caagtctgtt tggctgcggg ctttgaagcc gattttcaat
    65101 cttggcgtca ggccttaaaa gcggagtctg tctatcattt ttttcatgat atcattccag
    65161 tctttgaaaa actcgggcat gttgacctat cagataaact agaagagctt tatagcctag
    65221 cgagtcctca ggtgcagatt gcctccaagg gaggtctctt ggaaatccag tttgattttc
    65281 aaaatattgc ccaggaggaa atcgaccaag ccatgcaggc cttggttgcc aatcaggatt
    65341 tttatattga ttcgtctaat caagtttact ttttcgatga agaaaccaag aaaattcggc
    65401 aaaatctaca ggaactgggg caatttgaat taaaagatgg gaccttgcag gctcggaaat
    65461 ccttggccta cagtttagct catctctttg aagggcgaga ccgtgtttct ttttcacaag
    65521 aattccagaa tctggcccag gatttgacac atccagagga ctttcctcga caggcaactc
    65581 aggttcaggc tgacttgcga gattatcagg aaaagggaat tggctggttg cagatgctcc
    65641 atcattatgg ttttggtggg attttggctg atgatatggg acttgggaaa acccttcaga
    65701 ccattgcttt tttgactagt caagtgacaa aagaaagtcg ggttttgatt ctcgctccgt
    65761 caggtttgat ttacaactgg gcagatgagt ttcagaaatt tgccccacag ttggatgtgg
    65821 ctgttgttca tggtttgaaa gcaagtcgtg aggagattct tgccgagagc catcaaatct
    65881 atgtgactag ctatgccacc ttccgtcagg acagtgagtt ttatcagggg atggcctttg
    65941 acttcctttt cttagatgag gctcaggtca tgaaaaatgc ccagaccaag attgcccaga
    66001 ccttgagaca atttgtggtg ccgtcggtct ttgccttgtc aggaactccg attgaaaacc
    66061 atctgggtga gttgtggtct attttccaaa tcgtcatgcc cggacttttg ccaagcaaga
    66121 aagaatttat gaaattgcca gcagagcgag tggctcagtt tatcaagcct ttcgtgatgc
    66181 gacgcaagaa agaagaagtt ctgactgaat tgccagactt gattgaggtg gtttataaaa
    66241 atgaactgga agaccagcaa aaggctattt accttgccca gttacaacag atgcgagacc
    66301 atctggctca agtgtcagaa caggaatttc agcgaagtcg tgtggaaatt ttatctggtt
    66361 tgatgcgctt gcgtcaaata tgtgacactc ctgccctgtt tatggaagat tatcagggag
    66421 ccagcggcaa actagatagt ctccgagacc tactggtaca ggtggcagac ggcggacacc
    66481 gtgtcttgat tttctctcag ttcaagggaa tgttggaaaa aattgaacaa gaactgccag
    66541 acttgggcct gacatccttt aaaattacgg gttcaacccc agccaaggaa agacaagaca
    66601 tgaccaaggc ctttaaccaa ggagaaagag atgcctttct gatttccctc aaggctggtg
    66661 gggtcggtct gaacctgaca ggtgcagata cagtgatctt ggttgacctt tggtggaatc
    66721 ctgcggtgga agcgcaagcc attggccgtg cccatcggat gggtcaggaa gaaacggttg
    66781 aggtctatcg cttggtgacc aagggaacca ttgaagaaaa aattcaggaa ctccaagaac
    66841 aaaagaaaca tctggtgtca caagtattgg atggtacaga gtcacgtggt agtctgaccc
    66901 tagcagaaat tagagaaatt ttgggaattt ctgaagccag cacttgaaaa atcaagacaa
    66961 tacgctataa tgaagtgttg aaaggaaata gaaaatgaaa caagaacgat ttccattggt
    67021 gtcagatgac gaggtcatgt tgactgaaat gccagtcatg aatctctatg atgagtctga
    67081 tctgatcagt aatatcaagg gtgagtatcg agataaaaat tatttagaat gggctcctat
    67141 tactgaagaa aaactagtaa aaccgattga aaagcaagtc gaaaaaccta aaaaggctcc
    67201 tttaggggtt aaaaaagaag gaaagagcta tgcggaggtg gcgcgtgaag aagcgcgtgc
    67261 ggacttgaaa aagaaacgct ctgctaacta cctaactcag gatttcagcc ttgcgagacg
    67321 tcattctcag cccagtctag ttagacaggg caatcaaccg acaactcctt tccaaaagga
    67381 aaatcctggt gaatttgtca aatatagcca aaaattgacc cagtctcatt atatcttggc
    67441 ggaagaagtt cattctatcc ctaccaagaa tgaagaagtg tcagcacctg ctccaaagaa
    67501 aaacaattat gattttctaa agaagagcca aatctacaat aaaaaaagta aacaaacaga
    67561 acaagaacgt cgggttgccc aagagttgaa tctgaccaga atgacagaat aggggagaaa
    67621 acatgtcaaa gacatatcat tttatcggaa ttaagggatc agggatgagt gccttggcct
    67681 tgatgttgca ccagatgggg cacaaggttc agggatcaga tgttgaaaag tactacttta
    67741 cccaacgcgg tcttgagcag gcaggaatta ccattcttcc ttttgatgaa aagaatctag
    67801 acggtgatat ggaaattatc gctggaaatg cctttcgtcc agataacaac gtcgaaattg
    67861 cctatgcgga ccaaaatggt atcagctaca aacgttacca tgagtttcta ggtagcttta
    67921 tgcgtgactt tgttagcatg ggagtagcag gagcacatgg aaaaacttca acgacaggta
    67981 tgttgtctca tgtcttgtct cacattacag ataccagctt cttgattgga gatgggacag
    68041 gtcgtggttc ggctaatgcc aaatattttg tctttgaatc tgacgaatat gagcgtcact
    68101 tcatgcctta ccacccagaa tactctatta tcaccaacat tgactttgac catccagatt
    68161 atttcacaag tctcgaggat gttttcaatg cctttaacga ctatgccaaa caaattacca
    68221 agggtctttt tgtctatggt gaagatgctg aattgcgtaa gattacgtct gatgcaccaa
    68281 tttattatta tggttttgaa gctgaaggca atgactttgt agctagtgat cttcttcgtt
    68341 caacaactgg ttcaaccttc accgttcatt tccgtggaca aaacttgggg caattccaca
    68401 ttccaacctt tggtcgtcac aatatcatga atgcgacagc cgttattggt cttctttaca
    68461 cagcaggatt tgatttgaac ttggtgcgtg agcacttgaa aacatttgcc ggtgttaaac
    68521 gtcgtttcac tgagaaaatt gtcaatgata cagtgattat tgatgacttt gcccatcatc
    68581 caacagaaat tattgcgacc ttggatgcgg ctcgtcagaa atacccaagc aaggaaattg
    68641 tagcagtctt tcaaccgcat acctttacaa gaaccattgc cttgttggac gactttgccc
    68701 atgctttaaa ccaagcagat gctgtttatc tagcgcaaat ttatggctcg gctcgtgaag
    68761 tagatcatgg tgacgttaag gtagaagacc tagccaacaa aatcaacaaa aaacaccaag
    68821 tgattactgt tgaaaatgtt tctccgctcc tagaccatga caatgctgtt tacgtcttta
    68881 tgggagcagg agacatccaa acctatgaat actcatttga gcgtctcttg tctaacttga
    68941 caagcaatgt tcaataggat gttcccatgg aaattccaat taagattatt caggcaagca
    69001 agtctgattt gcctgagata ggggcacttc agacctcgtc ttttccagct gaaaagcagc
    69061 aactttccca tattttagaa gaaagtatcc gtaagtgtgc ggataccttt cttctagcta
    69121 gggatgaaaa tcaactttta ggctatattt tatcaagtcc ccagtcagac aatccgcaat
    69181 gtctaaaagt acattcttta gtcatcgagt ctgaccatca gagacagggc ctgggaacac
    69241 ttcttcttgc agccttgaaa gaggtggcag ttgagctgga ttacaaagtg attcgtttgg
    69301 agagtcctga tgagctgctt tcctattttg gaatgaacgg ttttgttgat gaagaagaaa
    69361 ctttgctcta tgcaactagc cagggctata gtatgatttg gtttaatccc ttttatctgg
    69421 aggaacaatg aaaatcagac aagcaagatt agaagatttg gatcggattg ttgaacttga
    69481 atttgaaaat ttttcggtcg aagaagccat tcctccttct gtctttgaag cacatttgag
    69541 agaaattcag acctcttttc tggttgctga aaaagaagga agaatcatgg gttatatcga
    69601 aggaccagtt gacctgcacc gccatctgca agaccagtct tttacagaag aaataaaaga
    69661 ctatagtcat gagcctggtg gttatatctc tgtgacctgt ctgtctattg ccaaggaggc
    69721 acagggactc ggactgggtc agaaattgct gacagccttg aaagaagttg ctcttgaaga
    69781 tgagagagat ggcattaatc taacctgtca tgactatctc atcgcctatt atgaaaaaca
    69841 tggatttgtc aacgaaggcc agtcccagtc aacctttgca ggggaaacat ggtatgatat
    69901 ggtctgggaa atgaaaaaat aagttaggaa aagtatcata gattgctttt tttggacttt
    69961 taagatataa tattatggat tatcaacaag gaggaaaaac ttttgagtga aaagtcaaga
    70021 gaagaagaga aattaagctt taaagagcag attctgagag atttagaaaa agtaaaaggc
    70081 tatgatgaag ttctgaaaga agatgaggca gtagttcgca ctcctgcaaa tgaaccttca
    70141 gctgaagaac tcatggctga ttccttgtca acggtagagg agattatgag aaaagctcct
    70201 accgtgccta ctcacccaag tcaaggtgta ccagcttctc cagcagatga gattcaaaga
    70261 gaaactcctg gtgttccaag tcatccaagt caagatgtac cttcttctcc agcggaagaa
    70321 agtggatcaa gaccaggtcc aggtcctgtt agacctaaga aacttgaaag agaatacaat
    70381 gaaaccccaa caagggtagc tgtttcctat acgacggcag agaaaaaagc agaacaagca
    70441 ggtccagaaa cacctacgcc tgctacagaa acagtggata tcatcagaga tacatcacgt
    70501 cgtagccgta gagaaggagc aaaacccgct aagcctaaga aagagaagaa gtcacatgtg
    70561 aaagcttttg tgatttcatt ccttgtattc cttgccttgc tctcagcagg tggttacttt
    70621 ggttaccagt acgtgctaga ttccttatta cctatcgatg ctaattctaa gaaatatgtg
    70681 acggttggaa ttccagaagg ttcaaacgtt caagaaatcg gtacgacgct tgaaaaagct
    70741 ggtttggtaa agcatggtct gatttttagt ttttatgcca agtataaaaa ttataccgac
    70801 ttgaaagcag gttactacaa tttgcaaaag agtatgagta cagaagactt actcaaagag
    70861 ttgcaaaaag gtggaacaga tgaaccgcaa gaacctgtac ttgcgacttt gacaattcca
    70921 gaaggttata ccttggatca gattgctcaa gctgtgggtc aattgcaagg tgacttcaaa
    70981 gagtctttga cagcggagac tttcttggct aaagttcaag atgagacgtt tatcagtcaa
    71041 gcagtagcga aatatcctac tttactggaa agtttgcctg taaaagacag cggtgcgcgt
    71101 tatcgtttgg aaggatacct tttcccagct acatactcta tcaaggaaag cacaactatt
    71161 gagagcttga ttgatgagat gttagctgct atggataaga acctatctcc ttactatagt
    71221 actatcaaat ctaaaaactt gactgtcaat gagttgttga ccattgcttc cttggtcgaa
    71281 aaagaaggtg ccaagacaga agatcgtaag ctcattgcag gtgtattcta caatcgtttg
    71341 aatcgtgata tgccacttca aagtaatatt gcaatcttgt atgcccaagg aaaactgggg
    71401 caaaatatca gtctagctga ggatgttgcg attgatacca acattgattc accttataat
    71461 gtttataaaa atgtaggtct catgcctggt ccagtcgata gtccaagtct ggatgcgatt
    71521 gagtcaagca tcaatcaaac taagagcgat aacctctact ttgtagcaga tgtcacagaa
    71581 ggcaaggtct actatgctaa caatcaagaa gaccacgacc gcaatgtcgc tgaacatgta
    71641 aacagcaaat taaactaaac aaactaaaat tatgtgatat ttcacataat tttctttaga
    71701 aaatattatc agaagaaagt tgagaaaaat ggcagaaaaa acatatccta tgacccttga
    71761 ggaaaaggag aaacttgaaa aagaattaga agaattgaaa ttggttcgtc gaccagaagt
    71821 ggtagaacgc attaagattg cccgttcata cggtgatctt tcagaaaaca gtgagtacga
    71881 agcagctaag gatgaacaag cctttgtcga aggacaaatc tctagcttag aaacaaaaat
    71941 ccgctatgct gaaatcgtca atagcgacgc agttgcccag gacgaagtag cgattggtaa
    72001 aacagtcacc atccaagaaa ttggcgagga cgaagaagaa gtttatatta tcgtaggttc
    72061 agctggtgcg gatgcctttg caggtaaggt ttcaaatgaa agcccaattg ggcaggcctt
    72121 gattggcaag aaaacaggtg acacagcaac aattgaaacg cctgttggta gctatgatgt
    72181 aaaaatcttg aaggttgaaa aaacagccta aaaacagaaa aaggagtggg gaggcgatgt
    72241 gcttcactca ctcctttttc cattttgcta ttcttcgaaa atctcttcaa accacgtcag
    72301 cgtcgccttg ccgtatatat ggttactgac tttgtcagtt tcatctacaa cctcaaaaca
    72361 gtgttttgag ctaacttcgt cagtttcatc tacaacctca aaaccatgtt ttgagccgac
    72421 ttcgtcagtt tcatctacaa cctcaaaacc atgctttgag ctgacttcgt cagtttcatc
    72481 tacaacctca aaaccatgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc
    72541 atgttttgag ctgacttcgt cagttctatc tacaacctca aaacagtgtt ttgagcaacc
    72601 tgcggctagt ttcctagttt gctctttgat tttcattgag tattagatgt atagtgcatt
    72661 gaagttaaaa tagtaaactt tgatttctaa gacattatta gaaattagat taaattcctt
    72721 aatcgatttg cgcatgtttt atttcatttc attatacttt agattggaaa ataaggaatc
    72781 acataaatcg caagaaatag ggaatctgat tcgtgtttat aaccgttcaa aaagggaaga
    72841 ggctgaaagt gagccactta atctttatgt cgaagatgaa aagggcaatc tccttgcggg
    72901 tttgatagca gagacttttg gaaattggct agaaatcgag tatttgtttg taaaagagga
    72961 actgcgaggg caaggaatcg gttcaaaact attgcaacag gcagaaagtg aagctaagaa
    73021 tcgaaattgt tgttttgcct ttgtcaatac ttatcagttc caggcaccgg acttttatca
    73081 gaaacatggc tacaaggaag ttttttcttt gcaagactat ctctacatta ggcaaagata
    73141 ttattaccaa aagaatctgt aaggtgagta tggaggtata atacaaagag aggctcgcac
    73201 ataagtatgg aggtgtactg atgtatatga gtaactatcg ataaatacca gttataacct
    73261 agaaattgtg tctttctaga cgatattgag gacaatataa tcgtagctcc aaaattagga
    73321 atcaaggctt atcaggttaa gaaaagaagt gatgttgttg atattttaaa acaatttatt
    73381 taaactaaaa actctctatc tttttaaatt gacattaatc cgagattccc ctctaggatt
    73441 ttgtgttgct ttcattaaat ttacaggtaa gacttgctag cttcagtaaa tatacacaag
    73501 tttttcagaa tagagtgaaa tccgaaagaa aatggaaata atgaaaactc ttgtaggcat
    73561 aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa acacaagaaa ggcacaga
//
LOCUS       DAYYQN010000002        72601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYYQN010000002 DAYYQN010000000
VERSION     DAYYQN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.002"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..229)
                     /locus_tag="ACHBRQ_000077"
     CDS             complement(<1..229)
                     /locus_tag="ACHBRQ_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="HFM9591843.1"
                     /translation="MLVNEMEITDHRVDNLFEKGKNEIKDSIGTNSVLNKKIILQKIR
                     KLSNQPSGYWIGSLDERFLDHAIINQIDVTSE"
     gene            complement(389..685)
                     /locus_tag="ACHBRQ_000078"
     CDS             complement(389..685)
                     /locus_tag="ACHBRQ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591844.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
     gene            complement(669..2801)
                     /gene="pfbA"
                     /locus_tag="ACHBRQ_000079"
     CDS             complement(669..2801)
                     /gene="pfbA"
                     /locus_tag="ACHBRQ_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HFM9591845.1"
                     /translation="MLKIVKKLEVLMKYFVPNKVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SVIKSNANGKEPVIRDSDGNFNIVTNNLSDKNEKEKNKEEKQSNSNNVIDSNQKNGEF
                     NSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKLPKTGSN
                     KIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3918..5144)
                     /locus_tag="ACHBRQ_000080"
     CDS             complement(3918..5144)
                     /locus_tag="ACHBRQ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFM9591846.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5187..5879)
                     /locus_tag="ACHBRQ_000081"
     CDS             complement(5187..5879)
                     /locus_tag="ACHBRQ_000081"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9591847.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNCRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(6052..7803)
                     /locus_tag="ACHBRQ_000082"
     CDS             complement(6052..7803)
                     /locus_tag="ACHBRQ_000082"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591848.1"
                     /translation="MQNKQEQWAVLKRLMSYLKSYGLLIFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKVLILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(7793..9538)
                     /locus_tag="ACHBRQ_000083"
     CDS             complement(7793..9538)
                     /locus_tag="ACHBRQ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9591849.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9744..10121)
                     /locus_tag="ACHBRQ_000084"
     CDS             complement(9744..10121)
                     /locus_tag="ACHBRQ_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9591850.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10193..10649)
                     /locus_tag="ACHBRQ_000085"
                     /pseudo
     CDS             complement(10193..10649)
                     /locus_tag="ACHBRQ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10783..11610
                     /locus_tag="ACHBRQ_000086"
     CDS             10783..11610
                     /locus_tag="ACHBRQ_000086"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9591851.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11622..11717
                     /locus_tag="ACHBRQ_000087"
                     /pseudo
     CDS             11622..11717
                     /locus_tag="ACHBRQ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
     gene            11765..12004
                     /locus_tag="ACHBRQ_000088"
     CDS             11765..12004
                     /locus_tag="ACHBRQ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HFM9591852.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12387..12482
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12882..13463
                     /locus_tag="ACHBRQ_000089"
     CDS             12882..13463
                     /locus_tag="ACHBRQ_000089"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9591853.1"
                     /translation="MKLLEERILKDGHILDDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVLFKEADL"
     gene            13463..14725
                     /locus_tag="ACHBRQ_000090"
     CDS             13463..14725
                     /locus_tag="ACHBRQ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9591854.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIFVASLLAIVLNAVLNHKKK"
     gene            complement(14776..15237)
                     /locus_tag="ACHBRQ_000091"
     CDS             complement(14776..15237)
                     /locus_tag="ACHBRQ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HFM9591855.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(15234..15998)
                     /locus_tag="ACHBRQ_000092"
     CDS             complement(15234..15998)
                     /locus_tag="ACHBRQ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HFM9591856.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(16179..16583)
                     /locus_tag="ACHBRQ_000093"
     CDS             complement(16179..16583)
                     /locus_tag="ACHBRQ_000093"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFM9591857.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(16653..18134)
                     /locus_tag="ACHBRQ_000094"
     CDS             complement(16653..18134)
                     /locus_tag="ACHBRQ_000094"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9591858.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKAQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKCDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGISLD"
     gene            complement(18153..19331)
                     /locus_tag="ACHBRQ_000095"
     CDS             complement(18153..19331)
                     /locus_tag="ACHBRQ_000095"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9591859.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19495..20502
                     /gene="galR"
                     /locus_tag="ACHBRQ_000096"
     CDS             19495..20502
                     /gene="galR"
                     /locus_tag="ACHBRQ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HFM9591860.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20548..21585)
                     /locus_tag="ACHBRQ_000097"
     CDS             complement(20548..21585)
                     /locus_tag="ACHBRQ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFM9591861.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21586..21939)
                     /gene="nmlR"
                     /locus_tag="ACHBRQ_000098"
     CDS             complement(21586..21939)
                     /gene="nmlR"
                     /locus_tag="ACHBRQ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HFM9591862.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(22183..23073)
                     /gene="czcD"
                     /locus_tag="ACHBRQ_000099"
     CDS             complement(22183..23073)
                     /gene="czcD"
                     /locus_tag="ACHBRQ_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HFM9591863.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            23206..23742
                     /gene="sczA"
                     /locus_tag="ACHBRQ_000100"
     CDS             23206..23742
                     /gene="sczA"
                     /locus_tag="ACHBRQ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HFM9591864.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            complement(23908..24714)
                     /gene="pnuC"
                     /locus_tag="ACHBRQ_000101"
     CDS             complement(23908..24714)
                     /gene="pnuC"
                     /locus_tag="ACHBRQ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9591865.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(25059..26579)
                     /locus_tag="ACHBRQ_000102"
     CDS             complement(25059..26579)
                     /locus_tag="ACHBRQ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9591866.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26572..27300)
                     /locus_tag="ACHBRQ_000103"
     CDS             complement(26572..27300)
                     /locus_tag="ACHBRQ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9591867.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(27315..28079)
                     /locus_tag="ACHBRQ_000104"
     CDS             complement(27315..28079)
                     /locus_tag="ACHBRQ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFM9591868.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(28083..28526)
                     /locus_tag="ACHBRQ_000105"
     CDS             complement(28083..28526)
                     /locus_tag="ACHBRQ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9591869.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(29208..29504)
                     /locus_tag="ACHBRQ_000106"
     CDS             complement(29208..29504)
                     /locus_tag="ACHBRQ_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9591870.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(29526..30590)
                     /gene="pepA"
                     /locus_tag="ACHBRQ_000107"
     CDS             complement(29526..30590)
                     /gene="pepA"
                     /locus_tag="ACHBRQ_000107"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9591871.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30991..31692
                     /locus_tag="ACHBRQ_000108"
     CDS             30991..31692
                     /locus_tag="ACHBRQ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9591872.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHGLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            31708..31818
                     /locus_tag="ACHBRQ_000109"
     CDS             31708..31818
                     /locus_tag="ACHBRQ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HFM9591873.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKAYTIKEIFKTAEELLN"
     gene            32115..33074
                     /locus_tag="ACHBRQ_000110"
     CDS             32115..33074
                     /locus_tag="ACHBRQ_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9591874.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            33064..34020
                     /locus_tag="ACHBRQ_000111"
     CDS             33064..34020
                     /locus_tag="ACHBRQ_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9591875.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYILLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILIGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            34017..34769
                     /locus_tag="ACHBRQ_000112"
     CDS             34017..34769
                     /locus_tag="ACHBRQ_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591876.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            34865..35830
                     /locus_tag="ACHBRQ_000113"
     CDS             34865..35830
                     /locus_tag="ACHBRQ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9591877.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(36055..36297)
                     /gene="yidD"
                     /locus_tag="ACHBRQ_000114"
     CDS             complement(36055..36297)
                     /gene="yidD"
                     /locus_tag="ACHBRQ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9591878.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(36297..37019)
                     /locus_tag="ACHBRQ_000115"
     CDS             complement(36297..37019)
                     /locus_tag="ACHBRQ_000115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9591879.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(37006..37575)
                     /gene="scpB"
                     /locus_tag="ACHBRQ_000116"
     CDS             complement(37006..37575)
                     /gene="scpB"
                     /locus_tag="ACHBRQ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9591880.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(37594..38322)
                     /locus_tag="ACHBRQ_000117"
     CDS             complement(37594..38322)
                     /locus_tag="ACHBRQ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9591881.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(38322..39056)
                     /gene="xerD"
                     /locus_tag="ACHBRQ_000118"
     CDS             complement(38322..39056)
                     /gene="xerD"
                     /locus_tag="ACHBRQ_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9591882.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(39053..39514)
                     /gene="cbpB"
                     /locus_tag="ACHBRQ_000119"
     CDS             complement(39053..39514)
                     /gene="cbpB"
                     /locus_tag="ACHBRQ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9591883.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(39511..40032)
                     /locus_tag="ACHBRQ_000120"
     CDS             complement(39511..40032)
                     /locus_tag="ACHBRQ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9591884.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(40008..40979)
                     /locus_tag="ACHBRQ_000121"
     CDS             complement(40008..40979)
                     /locus_tag="ACHBRQ_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HFM9591885.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(40976..41770)
                     /gene="racE"
                     /locus_tag="ACHBRQ_000122"
     CDS             complement(40976..41770)
                     /gene="racE"
                     /locus_tag="ACHBRQ_000122"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9591886.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(41977..42225)
                     /locus_tag="ACHBRQ_000123"
     CDS             complement(41977..42225)
                     /locus_tag="ACHBRQ_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9591887.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(42481..44106)
                     /gene="treC"
                     /locus_tag="ACHBRQ_000124"
     CDS             complement(42481..44106)
                     /gene="treC"
                     /locus_tag="ACHBRQ_000124"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9591888.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(44284..46251)
                     /gene="treP"
                     /locus_tag="ACHBRQ_000125"
     CDS             complement(44284..46251)
                     /gene="treP"
                     /locus_tag="ACHBRQ_000125"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9591889.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETLVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            46436..47146
                     /gene="treR"
                     /locus_tag="ACHBRQ_000126"
     CDS             46436..47146
                     /gene="treR"
                     /locus_tag="ACHBRQ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9591890.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(47179..47982)
                     /locus_tag="ACHBRQ_000127"
     CDS             complement(47179..47982)
                     /locus_tag="ACHBRQ_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591891.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(47995..48888)
                     /locus_tag="ACHBRQ_000128"
     CDS             complement(47995..48888)
                     /locus_tag="ACHBRQ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9591892.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIVEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            complement(48900..49631)
                     /locus_tag="ACHBRQ_000129"
     CDS             complement(48900..49631)
                     /locus_tag="ACHBRQ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9591893.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSEPNSVV
                     QKDEAKRLLKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQ
                     VSIKHKVPVFGGSTEMIAVGGLYNYGTNYEELGRQTARMLIHVLKGEKPENIAVELPE
                     KLELHTNQEMADALGIDISKLEGKE"
     gene            complement(50121..51047)
                     /locus_tag="ACHBRQ_000130"
     CDS             complement(50121..51047)
                     /locus_tag="ACHBRQ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9591894.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(51058..52125)
                     /locus_tag="ACHBRQ_000131"
     CDS             complement(51058..52125)
                     /locus_tag="ACHBRQ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9591895.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(52134..53060)
                     /gene="oppC"
                     /locus_tag="ACHBRQ_000132"
     CDS             complement(52134..53060)
                     /gene="oppC"
                     /locus_tag="ACHBRQ_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFM9591896.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(53060..54556)
                     /locus_tag="ACHBRQ_000133"
     CDS             complement(53060..54556)
                     /locus_tag="ACHBRQ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9591897.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(54623..56602)
                     /locus_tag="ACHBRQ_000134"
     CDS             complement(54623..56602)
                     /locus_tag="ACHBRQ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9591898.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(57184..58530)
                     /locus_tag="ACHBRQ_000135"
     CDS             complement(57184..58530)
                     /locus_tag="ACHBRQ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HFM9591899.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     LISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDIISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFHHLFLH
                     VVGNCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            complement(58995..60188)
                     /locus_tag="ACHBRQ_000136"
     CDS             complement(58995..60188)
                     /locus_tag="ACHBRQ_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9591900.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(60326..61768)
                     /gene="gtfA"
                     /locus_tag="ACHBRQ_000137"
     CDS             complement(60326..61768)
                     /gene="gtfA"
                     /locus_tag="ACHBRQ_000137"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9591901.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(61952..62119)
                     /locus_tag="ACHBRQ_000138"
     CDS             complement(61952..62119)
                     /locus_tag="ACHBRQ_000138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591902.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKVKLTWFEEI
                     FEEYKKSSWNL"
     gene            complement(62126..62959)
                     /locus_tag="ACHBRQ_000139"
     CDS             complement(62126..62959)
                     /locus_tag="ACHBRQ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9591903.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(62973..63839)
                     /locus_tag="ACHBRQ_000140"
     CDS             complement(62973..63839)
                     /locus_tag="ACHBRQ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9591904.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(63853..65112)
                     /locus_tag="ACHBRQ_000141"
     CDS             complement(63853..65112)
                     /locus_tag="ACHBRQ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9591905.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTSFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(65204..67366)
                     /locus_tag="ACHBRQ_000142"
     CDS             complement(65204..67366)
                     /locus_tag="ACHBRQ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9591906.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            67474..68334
                     /locus_tag="ACHBRQ_000143"
     CDS             67474..68334
                     /locus_tag="ACHBRQ_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9591907.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            68331..69266
                     /gene="birA"
                     /locus_tag="ACHBRQ_000144"
     CDS             68331..69266
                     /gene="birA"
                     /locus_tag="ACHBRQ_000144"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFM9591908.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            69412..70718
                     /locus_tag="ACHBRQ_000145"
                     /pseudo
     CDS             69412..70718
                     /locus_tag="ACHBRQ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(70822..70909)
                     /locus_tag="ACHBRQ_000146"
     tRNA            complement(70822..70909)
                     /locus_tag="ACHBRQ_000146"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70873..70875),aa:Ser,seq:gct)
     gene            complement(70916..70989)
                     /locus_tag="ACHBRQ_000147"
     tRNA            complement(70916..70989)
                     /locus_tag="ACHBRQ_000147"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70953..70955),aa:Ile,seq:gat)
     gene            complement(71025..71095)
                     /locus_tag="ACHBRQ_000148"
     tRNA            complement(71025..71095)
                     /locus_tag="ACHBRQ_000148"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71061..71063),aa:Gly,seq:tcc)
     gene            complement(71115..71187)
                     /locus_tag="ACHBRQ_000149"
     tRNA            complement(71115..71187)
                     /locus_tag="ACHBRQ_000149"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71152..71154),aa:Phe,seq:gaa)
     gene            complement(71191..71264)
                     /locus_tag="ACHBRQ_000150"
     tRNA            complement(71191..71264)
                     /locus_tag="ACHBRQ_000150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71228..71230),aa:Met,seq:cat)
     gene            complement(71275..71364)
                     /locus_tag="ACHBRQ_000151"
     tRNA            complement(71275..71364)
                     /locus_tag="ACHBRQ_000151"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71326..71328),aa:Ser,seq:tga)
     gene            complement(71375..71448)
                     /locus_tag="ACHBRQ_000152"
     tRNA            complement(71375..71448)
                     /locus_tag="ACHBRQ_000152"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71412..71414),aa:Ile,seq:cat)
     gene            complement(71462..71535)
                     /locus_tag="ACHBRQ_000153"
     tRNA            complement(71462..71535)
                     /locus_tag="ACHBRQ_000153"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71499..71501),aa:Met,seq:cat)
     gene            complement(71541..71614)
                     /locus_tag="ACHBRQ_000154"
     tRNA            complement(71541..71614)
                     /locus_tag="ACHBRQ_000154"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71578..71580),aa:Pro,seq:tgg)
     gene            complement(71630..71703)
                     /locus_tag="ACHBRQ_000155"
     tRNA            complement(71630..71703)
                     /locus_tag="ACHBRQ_000155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71667..71669),aa:Arg,seq:acg)
     gene            complement(71713..71798)
                     /locus_tag="ACHBRQ_000156"
     tRNA            complement(71713..71798)
                     /locus_tag="ACHBRQ_000156"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71762..71764),aa:Leu,seq:taa)
     gene            complement(71806..71877)
                     /locus_tag="ACHBRQ_000157"
     tRNA            complement(71806..71877)
                     /locus_tag="ACHBRQ_000157"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71843..71845),aa:Gly,seq:gcc)
     gene            complement(71900..71972)
                     /locus_tag="ACHBRQ_000158"
     tRNA            complement(71900..71972)
                     /locus_tag="ACHBRQ_000158"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71937..71939),aa:Thr,seq:tgt)
     gene            complement(71991..72072)
                     /locus_tag="ACHBRQ_000159"
     tRNA            complement(71991..72072)
                     /locus_tag="ACHBRQ_000159"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72036..72038),aa:Leu,seq:tag)
     gene            complement(72078..72150)
                     /locus_tag="ACHBRQ_000160"
     tRNA            complement(72078..72150)
                     /locus_tag="ACHBRQ_000160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72115..72117),aa:Lys,seq:ttt)
     gene            complement(72175..72247)
                     /locus_tag="ACHBRQ_000161"
     tRNA            complement(72175..72247)
                     /locus_tag="ACHBRQ_000161"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72212..72214),aa:Asp,seq:gtc)
     gene            complement(72250..72322)
                     /locus_tag="ACHBRQ_000162"
     tRNA            complement(72250..72322)
                     /locus_tag="ACHBRQ_000162"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72287..72289),aa:Val,seq:tac)
     gene            complement(72327..72442)
                     /gene="rrf"
                     /locus_tag="ACHBRQ_000163"
     rRNA            complement(72327..72442)
                     /gene="rrf"
                     /locus_tag="ACHBRQ_000163"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(72519..>72601)
                     /locus_tag="ACHBRQ_000164"
     rRNA            complement(72519..>72601)
                     /locus_tag="ACHBRQ_000164"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT        21696 a        16282 c        13385 g        21238 t
ORIGIN      
        1 gttcactagt aacatctatt tgattaataa tagcatggtc taaaaatctc tcatccaacg
       61 aacctatcca gtacccactc ggttgattag ataattttct gattttttgt aaaataattt
      121 ttttatttaa aacactattt gtaccaattg aatcttttat ctcatttttc cctttttcaa
      181 ataagttatc tactctatga tcagttattt ccatttcgtt tactaacatg acgcagtcac
      241 ctagcatcat atactccaac tttttttctg aaagtttagc aaatattggt aagcgataat
      301 atagtatgtt gaaactagaa tagtacacct ctacttctaa aacattgtta gaaatcgatt
      361 tgactgtcct gattgatttg tcctattatt atttcatttt actatactct gttaatttat
      421 atgagtttaa accgatttca tctttaacct cgagtaaagc agtttcaaat atttgtttaa
      481 gagtttttga ttctttacaa ttaaccgaca aactttctga taaaatatgt acaacttctg
      541 agactgaata acctatctcc tctttagaat tatataaatc tgtagctcca ccaataatcc
      601 aaaaatactg attttgtgaa cctacaatat cctcattttc tacggaactt ccttgtatcg
      661 aacaaatttt atttatcttt accataatac ttcaaccctt ttagtgtcaa aagtaaacca
      721 attcctgtca ctgttaagaa tagttccata atcttgttcg aaccagtctt tggtaatttt
      781 tgttttacat ctactatttc tttagattta ttaatatgat tttcagtttc tctgccatct
      841 ccaactattt tatagtttac ttcttctgtc ttattatctt gtttattgtc gatcttgtca
      901 ttcatttgtc tattatcttt acttgagtta aactctccgt tcttctggtt actatcaatt
      961 acattatttg aattagattg tttttcctct ttgttttttt ctttttcgtt tttatcactt
     1021 aaattatttg ttacaatatt gaaattacca tcgctatcac gtataacagg ttctttccca
     1081 tttgcattag atttgatgac tgatatatac ttaccggata aattataaaa ttggttattt
     1141 aaaacggtta ttttaccctt tgaatcctca ataacaattc cttctttacc accgaatata
     1201 ctattctctg agactactaa attatcacta actcgtaata attcaatatt tggttgttct
     1261 gtttccttag aattattatt aattacattt tttgttacag taatatctga tacttttcct
     1321 aaatattctg cactatcttt tgcaactcgt atcgcttttg ttttaggatc ggcaatatta
     1381 aatatatttt cggcgataac cacctgttta tttaaatcta ataggtcttt agtgttttta
     1441 tagctataag catttactaa agctgctccg ttttctcgat aatgtacact ctctccttta
     1501 actttcttat caaagcgatt tcctttgatt aatacatcag tgaatcctgt aaaacgtaca
     1561 cctgcataca tcatgttatc aaaatgatta tttaaaattt taatattaga ggggttctgt
     1621 gtcgacaatg tttgatagtg tgtaccaatt gctgttacta attccccaga tttatcactt
     1681 ttgccaaaat aggaattttg aatagtcaca ttttcagatt ttttcccatc atcattcaag
     1741 gcataaggaa aaccttttct agttaatggt tcaatctgaa tgctctcctt acttatgatt
     1801 tgcccatcct tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt atcaactaat
     1861 acatttttcg aacctgcaat ttgaatagca tgcccttgat aactatcctt gaatgttaca
     1921 ttttttatag ttacgttatt tgaattccca atagcaaatg cacctgaaga atttataagt
     1981 ggtagatttt ttgctttagt tccttcttca ttcaaagcac cgttcatatc aatcgtacca
     2041 ccagaataac taatatcttc tgttgggccc cattctactt gcgcaccatc atctgtaaat
     2101 aaacctgtca taaaaacaat ggaagggtga ttcttaatat ttataccatt tagaattgta
     2161 gctttctcat tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc ttttactaaa
     2221 taagttcctt caggaaaata tacatttcct ccacctagcc cttgagctgc agcatctatt
     2281 gcatcttgaa ttgcttgtcg atcatcatga atcccatcac ctaccgcacc ataatctttt
     2341 acacttaaag cagtatcttc aaattctttt ttgataattc tgtcacttat aacatcttcc
     2401 ttataactct tattttcatt agtagatgtt actgtagatt caactgttct gttgtctaat
     2461 tgtgaattgg aacgaatagt accattagag ctgatactat taacacttaa cgaattatct
     2521 aaaccattag tggttgaatt agaggcaatc atttcagcag aacgattttg attaacagtt
     2581 ggtgaattat ctaaatcatt agtaattgca ttagaagact cttttgtagc catcggtgaa
     2641 gaactagtaa caacttcatc cgataaaata ccttggcttc ccaaaattga aattgccaat
     2701 agtaccgagc aagtccccac ctttaattta cgaatactga ataccttatt aggaacaaaa
     2761 tatttcatca gaacctccaa tttttttact atttttaaca attgattatt acttttctta
     2821 aaatagatag aatgacaccg ctgtcattat ctttatcata tattttccaa attgaattgt
     2881 caaggaataa cacaagaaaa tattaaaatt tatactcttc ggaaatctct tcaaactagg
     2941 tcagtgccgc cttgccctaa gtctaggtta ctgacttggt cagttctata gtagactgaa
     3001 actataatag tacacctcta cttctaaaac attgttagaa atcgatttaa ctgtcccgat
     3061 taatttgtcc tattcttatt tcattttatt atatctacaa cctcaaagca gggttttgag
     3121 ctatctatgg ccaacttcct agtttgctct ttagccttcc attcaaaagt gatgctatct
     3181 ttcttagctt gaatataaaa tatcccattt actatattta aatttgttga tatgttgtaa
     3241 attataaatg aaagaacaaa tatcttgtca tacaagcatt cgccagggat atttacatga
     3301 atatcgctca tatggcggat tgaaactaaa acactacaat atatttctta aatagacctt
     3361 ctataaaagc tagatttaac ctcgataaaa atagatgcag tgcttctaaa atactggaat
     3421 agggcatata ggtaatcgta gacaagtgtt ttatagattg tctcgcagga gatgcaagca
     3481 tcactaaaca tatagagtat ttgtattatt ataatatcaa catgctataa attctatcat
     3541 gcaaaagaat gactagaaca tgaaagacag gcacaaaatt ttgaacaata ttctgaaaac
     3601 aaaaaaagac gcgagtttcc acaatctcac tcatggaaac tcacgtcttt caaaaaatag
     3661 ggtgaattga aaactagaac aagacactag attttctaaa tgatgtctag taaggattct
     3721 aaatgctaca ctaaattgct cacatcttat ttcaaaccac gggactccca caagaaagga
     3781 aaatcctata aaaattctct ctctgaggag aaagaggcct caactacagg tgtaaactat
     3841 tatcaattga aaacaacagt ttttacactc aattagactt tttagggaca ggatcacttt
     3901 cgcttgactg tagtttgtca tttttttgat aaagttagca ctttttcaga aactgttttg
     3961 aaagtctcaa tgatatagtc tacttcttca tcgcttaatt tagtatgaag agggagggta
     4021 atttcattct caaagaaggc ataggcctta ggatagttcg tcatatcaaa tccaagattc
     4081 ttataggctg tcaagagagg aagcggtttg tagtgaacat tacttgcaat tcctgcttta
     4141 gccaattctt ggatgatgag gttgcgttct tctaggcttg ctccttctac acgggtgatg
     4201 tagaggtggc gtgaagattc gacagtttca gtcttgtgtg ccaaaggatg gatgcgagaa
     4261 cctgcaaaac cactatcata gcggtccaca atgtccttac ggcgttgcaa caaacttgga
     4321 tagcggtcca attgtaccaa accaagtgaa gccatgatat cggtcatgtt gcacttatag
     4381 gctggtgtaa cgatatcgta ttcccatgac cccaattgca tcttggcaag agcatcctta
     4441 gtttgcccgt gaagggaaag gatttggaat tccttgtaca tctcttcgtc atcaatcact
     4501 ggattggctt tccaagtcgc acttccacct tctgccgttg taaagttctt aacggcatgg
     4561 aatgagaagg aagtaaagtc agcgatagaa ccagaaggtt gtcctttata agtagatccc
     4621 aaagcgtggg cactatcaga gacaatgaca atacggttaa aggccttttg ccacttgctt
     4681 gaagcggtaa agaagtcacg ttttttctcc acgacttgga acaaacggtc ataatcgcaa
     4741 acaatccctg cgagctctac tgggataatc accttagttt tctcagtgat agcttgctca
     4801 agcaggtcat agtccatctc aaacgtatct gcttggatat ccaccatgac aggggttgct
     4861 cccacgtgcg taatgacact acatgaagcc gtataggtca tggctggaac gatgacttca
     4921 tcaccaggtc ccacttccaa aacgcgtaaa atcaactcca gagcggctgt cgcagagttg
     4981 agacaaacag tcttaggtgt ctgtgtgtaa agagacaagc ggcgctccag ttcttttgtt
     5041 ttaggacctg ttgtgatcca accagaacgc agggtatccg ctacttcagc aatttctgct
     5101 tctgtgatat caggcggtga aaatggaata ttgtaatttg gcattgattt gctcctttta
     5161 tctgtactca ttttcttatg actactttat tttagtacct caaacacggt ttgaaacatg
     5221 attttgatgt ccccaaagaa actaaactct cggagatagg cgaggttata gcgcatcttt
     5281 tcaggaagaa catgctccac ataggcctga tcaactgaca gacctttctc cgtcatttga
     5341 ctgatgatgg tgtcctcatc cttgtagttg atgctggctg gagaggtaat tcctgcttgc
     5401 aagagcaagg ttgccatcat ttcagggcta tactgctctg tataacgtgg cacttcaggt
     5461 cgtgtaccga caaaggacat ctcaccttta aggacattga ccaactgagg cagttcgtcc
     5521 aaacggacac gtcggatgaa atttccaacc ttggtaatac ggctatcgtt agcagaagtc
     5581 accagacttc cttttttatc cgcatccgtc accatggtac ggaacttcca aatcttgaac
     5641 cgacagttgt actgggtcac acgctcttgc ttgtaaatca ctggcccctt gctatccaac
     5701 ttgatccaaa tgctcaagat gagaaagatg ggagaagtta gaaccagtaa gaccaaggcc
     5761 aaaacccagt ccaagcaacg cttgaaaatc agcgaaccct tccttttaga gacaagctgg
     5821 tagtaagact caacctcgct tgatttcatt tccacaggca agtcttccca tttcagcatg
     5881 ctgttctcct ttgtttttat tatactattt gtcaacattt ttcattatac cacaaaatgg
     5941 aaaaggcggt gaaagaaatc tgtaatttga aggatttcct tctagtactc aatgaaaatc
     6001 aaagtacagc tttgagattg cagataaagc tgacatggtt taaagagatt ttcaaagagt
     6061 atcggccatg gcccctgcct gcaaactata catcttgtga taagttcctc ccagagccaa
     6121 gagttcctca tgggttccac tctcgataat gcgtccctta tccaagacat agatgcagtt
     6181 ggcgtcttga atagtagaaa ggcggtgagc gatagcaata gttgttcggc cctgtctcat
     6241 cttcgccaga gaagcttgaa ccaagctttc tgtttcagag tcaatattgg ctgtcgcttc
     6301 atccaaaatc aggactttag gctggctggc gactgttcta gcaaaggcaa gaagctggcg
     6361 ctgcccagta gagaagctcg aaccacgctc ggaaacaggg gagtcgtacc cctgaggaag
     6421 ttcttgaata aaggaatctg catccacaaa ggctgccgca gcctgaacct gctcatcact
     6481 gatttcttgg tacatggcaa tattggactt aatagttcca tgatagagga agggttcctg
     6541 caagaccaaa ccgatgtttt ttctcagctc ttcttgactg aaatccctga tatccacatc
     6601 atccaagaga actctccctg actggaattc ataaaagcgc atgaggacat tgataataga
     6661 cgatttccct gaacctgtat gacctacaaa ggcaatggtt tcacccttat taacagagaa
     6721 agaaatgtca tccagaatcg gatgtttacc gtcatatgag aaacacacat gttcaaaacg
     6781 gatattgcct tcttggactt tggcttgccc attttcttga agaggttcat aggtcctctc
     6841 gtctatcagg gcaaagacac gacctgcaga aaccatagcc gtttgcagag ttgaaaagtt
     6901 ttgcgtcacc tcaatcaagg ggtcaaaaag gcggttgatg tactggataa aggcatacat
     6961 ggtcccgacc gttatcccga tagaaaaacc acggtagcca aagtaggcca tcaagactgc
     7021 atagcctaga agtttcagca aactcatggc aggtctcaaa aagagggcat ccaaggctac
     7081 agaacggttg gcgtagacca agtgttcttg gttgatttca tcaaattctg cctgcaggcg
     7141 cttctcttga ttaaaagcct gaataatcct gattccctcg atattctctg ccagcttact
     7201 attgatatct gacaagagac ttctggtttt ctcgatgatt ttcactgact tttttcgata
     7261 gagattgacc aaaaggaaaa tcaaaggaag aaagagcaag actaaagccg tcaaacgaaa
     7321 atccagcacc aacatggtat aaagggttgt cagaaagata aaaactgctg agataaagct
     7381 ggataaaatc ccagaaaaca tatcactaat cgtctcggta tcgttggtca aacgagaaac
     7441 gatagaacct gctggcgtct tgtcaaagta agacatgccc agtttctcca tattggcaaa
     7501 ggcatcccga cgaatatccc taacaatact gtaagacacg cgcgcaaaga gaagattgcc
     7561 tacatactga actacagttt gtaggatgta gagaccatag tagaccagca aaacggtaac
     7621 ggctagttgg ttaagattgc tgagatactg gtcgataaag tgggaagcca cgaggggtat
     7681 gacactttta atgaccgtcg tcgctaggag aaaactgagt gccaaaaaga tcaggagtcc
     7741 ataagacttg agataagaca tcaagcgctt caatacagcc cattgttctt gtttattctg
     7801 catcttcttc tcctttcatt tctaactgct gagactggta ggtttgggca taccagccat
     7861 ccatcgctag caagtcttcg tgcctgcctc gttcgataat ttgaccattt tgtagaacta
     7921 aaataaaatc tgcatggaca acagcactga ggcgatgggc agtgataatg gttgtcttgt
     7981 cctttcgcgt ctccttgagg ttgtcgataa tcgcatactc tgtcttggca tctacggcgg
     8041 ataaggaatc atccaaaatc aagatatcag ggtttaaaat catagcccga ctcatagcca
     8101 accgttgctt ttgaccacca gaaagactga ctcctttttc accaatcagc gtatcaaatc
     8161 cttgaggcat gtctacaata tcttggtaaa ctcgggctag cttagtagct tcctcgaccg
     8221 ctgaaagggg caagttagga ttgccaaagc ggatattgtc taggattgaa gttgcaaaga
     8281 gaaactggtc ctgaggaaca tagcccatga gactgcgaag gtctgtcaga cgatagtccc
     8341 gaatatcgtg accgtttaga taaatggcgc ccttatccac atcgtattca cgcaagagaa
     8401 gcttgattaa ggacgttttc ccagagcctg tctgcccaac caagcccagt gtttgccctt
     8461 ttgccaaact aaagtgaata tccgtcagtg tttcctcatt ttcaaaagca aagctgtcaa
     8521 tggcatactc caaacgccca ttttcaatac catccagagg aaactcaggg tcttgtacag
     8581 gagattcctg agacaaaaga ttttcaatcc gctggtaaga aaccttccct cgctgagtag
     8641 tattaaagag gaagccgatg gccataagag gccagaccag catatccaaa tagctgataa
     8701 aggtgaccag attcccaact gtaatctgcc cttcctgaac catcaaggag ccaaccaaaa
     8761 gcgttaaaac ataggacgaa ccaacaaaca agagaaccat agggtcaaag agactatcat
     8821 atttcatggt ttgcaggttc ttttggaagg ttaattcatt gactgcctga aaagacttca
     8881 actcgtctgc ctgataaccg aaagacttgg tcactttgat acctgatacg gactcctgta
     8941 ccttgttatt gagttcagaa aaagcagctt gggattcgcc aaaggcctta tgagtctttc
     9001 tccctaggcg actagtcgca taggccatga aaggtagggg gagaatggca acaagagtca
     9061 tctgccatga gatgctaaag agcatggtca acaaagtcac cagagccgtg atagaggcat
     9121 ccaccgcaga catgacaccg ccacctgcta aacgagtcaa ggcattgata tcattggttg
     9181 cgtgtgccat cagatcaccc gtccgatagg tttgataaaa ggctgacgac atttttgtga
     9241 aatgcttaaa caagcgagac cgcatgatct gtcccaagca ataagaggtc ccaaggatat
     9301 acatacgcca cacatagcgc aaatagtaca taccaaaggc tgcaagtagc aagtaaaata
     9361 ggctaagaag gaggtcctgc tgggttaatt gccccgatgt gatggcatca atgacccgcc
     9421 ccataaccat aggaggaatg agattgagga cggaaaccaa gatcagggcc acaattccga
     9481 ctagataacg gcgtttttct aacttgaaaa accaccaaag tttttgaata atggacataa
     9541 aatccctttc tgattgcaaa tggaaacctg aggccaatac tcaatgaaaa tcaaagagca
     9601 aactaggaaa ctagccgcag gctgctcaaa gcactgcttt gaggttgtag atagaactga
     9661 cgaagtcagt aacctacata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa
     9721 gagtacaaga ccccaggttt ttcttattca taagttacga ctgtaacagc acccttgtca
     9781 tattcagcaa taaagatatt ggctacattg tcatgccctt gtttactgag gttatcaagc
     9841 aaccactctt cgctacgacc aatcgattcc aagacatcta cttgaatcac accgtcagtc
     9901 acaactggat acttaggatt ttcatctccc atttgcacaa cgatgagttg cccattttgc
     9961 tcttgcacag ctcgtttgac ttgcttcatc tggaaaatcc cttggctacg aagtttgagg
    10021 gatacatccg atgcagacaa accaactgaa cgacaggctt ctgggtcaat attcccattt
    10081 ttgataagga gagttggctt accatcaatc aaacgtttca caaagtgaac attgttgttg
    10141 agctatttga gagtcaagat cagtttgatt aaaatttcga tataattaag tgtcatttgt
    10201 tcacatccac cagttcaatt gcgtctgtct tatacaattc gactttctct aacaggtact
    10261 tgtctggctc acttccgttt agggcacgat aatagcgatc tcccaccttg ataagtgcgc
    10321 catctgtgtt tgtggaagta ttaacataga cttctgactt gtctactccc aaatctttgg
    10381 aaacaacctc gatgaaatga agtgaagttt gaaatttatt gttagaaact tgattgtttt
    10441 gataagtcgt gatgctgacc aaaagcatag ctaaataagg ccagagctga aattatgacc
    10501 aactcacgaa atttggttcc ttttttatca tggtatgcct tgaaagcaaa aaaaactgtc
    10561 acagctaata aaacaatccc aaagccaacc atgataccat tttgctgacc gatttggctg
    10621 agtacatagt cataagagta gaatttcatg tattttcact ccctttcata aaatcattct
    10681 taattataaa cgaaagagag taatttttca aaccatacgt tggggaaata gatctttttt
    10741 tggtataata aaatctataa tctgaatgaa aaaggtaact ttatgaaact tatctcatgg
    10801 aatattgatt ccctaaacgc tgccctaact agtgactcag ctcgtgccaa attgtcccaa
    10861 gaagtcctac aaaccttggt cgctgaaaat gctgatatca ttgctatcca agaaaccaag
    10921 ctttctgcca aaggacctac aaagaaacac gtggaaattt tagaagaact cttcccaggc
    10981 tacgaaaaca catggcgctc ttcccaagag cctgcccgta aaggctatgc tggaaccatg
    11041 ttcctttata agaaagaact tacgcctacg atcagttttc cagaaatcgg tgccccttct
    11101 accatggact tggaaggccg tatcatcact ctagaatttg atgcattttt cgtaacccaa
    11161 gtttacactc caaacgctgg tgacggtctc aaacgcttgg aagaacgcca agtctgggat
    11221 gtcaaatatg cggagtattt ggctgaacta gacaaagaaa aaccagtcct tgcgaccggt
    11281 gactacaacg tagcccacaa tgaaatcgac cttgcaaatc ctgctagcaa ccgccgttca
    11341 cctggattta ctgacgagga acgtgctgga tttaccaacc ttttggcaac tggatttaca
    11401 gacaccttcc gccatgttca tggcgatgtt cctgaacgct acacttggtg ggcacaacgc
    11461 agcaaaactt ctaaaatcaa caatacaggc tggagaatcg actactggct cacaagtaac
    11521 cgtatcgctg acaaggtcac taaatctgat atgattgact caggtgctcg ccaagaccat
    11581 acaccgattg tcttggaaat tgacttgtaa ggattttcta tatggactac aatgcggtca
    11641 ttcccgagtt tcttgtatcg aatatcgaac agtcccggtc cttctactgt ggtttactgg
    11701 gttttaggat tgaatactaa cgtccagaag aaaactttct ctttctcttg aagagtgtca
    11761 actaatgtta gaggagggaa caaaggatca actagctgaa ctgacctacc cctttggtcg
    11821 tggagtcaat ctctcctttg gcataaagga tgttccaaaa ctctatcaaa aagtgatgga
    11881 ggcaaactat cctatttatc gtctcttgac caaaagaaag tttcgtgtca gtgatcccta
    11941 tatctatcct cataaatttg cagttttgga tccagatggc tattttttaa gatttagcga
    12001 gtagaataat aaaaattggg aaaagtcttt aaagttagaa aaaggcatat tgtcaggtgt
    12061 ctaaaaactt gatagtatgc cttttatgat ggaaaaatct tctaatactc ttcgaaaatc
    12121 tcttcaagcc acgtcaacgt cgccttaccg taggtatatg atactgactc tgtcaattct
    12181 atccacaacc tcaaaacagt gttttgaggt tgtggatagc ttcctagttt gctctttgat
    12241 tttcatttag tataaatgtt agcttacaat ggagtttttg ttcaccctct cttctttttg
    12301 ttcgcttttt aaaccgaaac actaacaaaa tagttgattt ataaagttat ttagtgtaaa
    12361 ataaaaacat tggctcaagc ctatttaaaa ttgaatatcg ttttacttgt ttatgtcgtg
    12421 aattggcacg acgtttctac aaggtgccgg aacacctaac aataagtaag tcagcagtga
    12481 gatatggccg tttttgccat gtctatttag gggacttact tagagattaa gtaggtcttt
    12541 tttgaatatt tttaatattt cccttaatta gtgcggacgg gaggtgaaat tatcctgtga
    12601 ataatttcag gaatccaggg aatttcatcc ctaatttttg agcccctagc tctttcattt
    12661 ctaggcttag gctaaaatag ttccccgaac tattttactc tcaaaaatcc cgtcatagac
    12721 aataacaaga ttattgtcta tgataccact acaacgggaa atatttgata agactcgctt
    12781 cgctcgtaat agttttatca aatatatagt attagcaaaa gggatcacta tagaaatatt
    12841 taaacaacaa gtaaattaac ttttagactt taggaggtct tatgaaatta ttagaagagc
    12901 gcatcctcaa ggatgggcat atcttggatg ataacatcct caaggtagat tcctttttaa
    12961 cccaccaagt tgactttagc ttgatgcgag agattggtaa ggtttttgcg gaaaaatttg
    13021 ctgctgccgg cattaccaag gtcgtaacca ttgaagcgtc gggtattgcc ccagccgttt
    13081 ttacagctga agccttaaac gttcccatga ttttcgccaa aaaagctaag aacatcacca
    13141 tgaacgaagg catcttaact gctcaagtct actcctttac caagcaggtg accagcactg
    13201 tttctatcgc tggaaaattc ctctcaccag aggacaaggt tttgattatc gacgatttcc
    13261 ttgctaatgg ccaagctgct aaaggcttga ttcaaatcat cgaacaggcc ggtgccacag
    13321 tccaagctat cggtatcgtg attgagaaat ccttccaaga tggtcgtgat ttgcttgaaa
    13381 aagcaggcta ccctgtccta tcacttgctc gcttggatcg ttttgaaaat ggtcaggtct
    13441 tatttaagga ggcagatctc taatgcaaac tcaagaaaaa cactcgcaag cagccgttct
    13501 gggcttgcag cacttactag ccatgtactc aggatctatc ctggttccca tcatgattgc
    13561 gacagccctt ggctattcag ctgagcagtt gacctacctg atttctacag atatcttcat
    13621 gtgtggggtg gcaaccttcc tccaactcca actcaacaaa tactttggga ttggactccc
    13681 agtcgttctt ggagttgcat tccagtcggt cgctcccttg attatgattg ggcaaagcca
    13741 tggtagtggc gctatgtttg gtgcccttat cgcatctggg atttacgtgg ttcttgtttc
    13801 aggcatcttc tcaaaagtag ccaatctttt cccatctatc gtaacaggat ctgttattac
    13861 cacgattggt ttaaccttga tccctgtcgc tattggaaat atgggaaata acgttccaga
    13921 gccaactggt caaagtctct tgctttcagc tattactgtt ctgattatcc tcttgatcaa
    13981 catctttacc aaaggattta tcaagtctat ctctattttg attggtctgg ttgttggaac
    14041 tgccattgct gctactatgg gcttggtgga cttctctcct gttgcggtag ctccacttgt
    14101 ccatgtccca actccactct actttgggat gccaaccttt gaaatctcat ctattgtcat
    14161 gatgtgtatc atcgcaacgg tgtctatggt tgagtcaact ggtgtttatc tagccttgtc
    14221 tgatatcaca aaagatccaa tcgacagcac gcgccttcgc aacggttacc gcgcagaagg
    14281 tttggccgta cttctcggag gaatctttaa caccttccct tacaccggat tttcacaaaa
    14341 cgttggtttg gttaaattgt caggcatcaa aaaacgcctg ccaatctact acgcagctgg
    14401 tttcctggtt ctccttggac tgcttcctaa gtttggtgcc cttgcccaaa tcattccaag
    14461 ctccgtcctc ggcggtgcca tgctggtgat gtttggtttt gtatctattc aagggatgca
    14521 aatcctagcc cgagttgact ttgctaacaa tgaacacaac ttccttatcg cagctgtttc
    14581 aatcgctgca ggtgtcggtc tcaacaacag taatctcttt gtcagcatgc cgacagcctt
    14641 ccaaatgttc ttctcaaacg gaatcttcgt agccagccta ctcgctattg tcctcaatgc
    14701 cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt tgtgtgacaa
    14761 aataaaaaca tctacctatt catatatgct agtgtcatag tcctgaaaat catctgcatc
    14821 aagaacaatt tcacaactct tatacttttc tcttacaagt cgttcggctt catctggatt
    14881 ttcagcctct atacttacta aacgtgataa agtttctgta atttctactg tatacttttt
    14941 cagttttaga ttgctttcca ataattcacc tattaacaat aatctatgat aggagctatg
    15001 tagccactct ggcaatttat ctcctacttc ccacccatat ttttccttca tcaaagctag
    15061 ttgtttttcg tcaccaaatt tcatattttc agataaccat ctcacacaat cgggatctga
    15121 taaggtacag acgattaaac ttaggttatc gtaacggctt tcagtttcat aatcaggcca
    15181 gatgactttt aagaaatcat acacctcttc tgatgcaaca agttgtttcg ttttcataat
    15241 ttccgatact ttcctctacc tttaaattct attatttctt tgtctcttaa tatttgaagc
    15301 tgttgcctaa tcttttcttt gatatgattg ttcttaacaa agatattttt taggtcactt
    15361 tcaaaacgat acatatcttc aagggtaaat tctgaaccct ctatcttatc tatacaattt
    15421 agaatttcta ttgtccaacc tcttgatgac agagagctct tccttaaaaa taaaccttgc
    15481 ttaaattctt ttgtaacttt ttctggatct ctaacttgtc catcttgcac aagaaatatc
    15541 cttcctttag aaggtacttg tgataaatca atgttacaac caatccaacc tgctcgtcta
    15601 gcagttggtg caagtggttt tctttgaata attgatttcg gtgtaacaaa ttgcttcgga
    15661 aggacaagaa agttatttac ctcaaaattt tttgtgtaag ttaaaaaaaa gaaattagga
    15721 ttattatctg cctgcacacg cttcatcatc gttgcataag caccatcatt gattgttgat
    15781 gaaaaatttc cttttttgct ctttagttca aactcctcac tacaatgatt acagtaaaaa
    15841 tctgctacag gccgattatt ttcaaaatga tttaagggat tgttcccaca attagggcaa
    15901 taagactgac gataaaccca atcctccgtt aagatacgtg ccttttggct attactttta
    15961 taggtttcta ctaattctaa attaaagtgt aattccattt aaaaattcct cactaatatg
    16021 agaactcact cgatataagc aattaaaatc tactataact agtataccac atatatgtac
    16081 ctattacatt tgtatttcac cactctcttt tatatttcct tctaacatat ctcataaatt
    16141 aaaaatgagt gagataaatt tatcccactc acctttcttt atatccttac ccgtctttcc
    16201 tcagaccttt ggcctgtgac aaacatggta aaggttgctt tacagacatt tctgccctct
    16261 tgattggtga tatccacatc catcacacag gttgtacgac cttgatggac acattctcct
    16321 ttaatggtca atacatcgtc gagttttcct gccttaaggt agttgataga ggattggagt
    16381 gtcaccccat caagtcccag cgagataacc accaaaccac tgatttggtc acatagcgtg
    16441 aatagatagc caccatgggc attgccatag tagttgagcg acgagttcac tactttggtc
    16501 gtcaccacaa cgtgaccatc tctcattttt tcaatttcgt aattttcaaa ggcagatata
    16561 gcgtcaaaat gaaagtcttt catcgtttcc tcctgatatt tcaaacgaca ctatgatact
    16621 actttttata ggactgtgca aggagaaagc tcctagtcta gtgaaattcc gacctgttcc
    16681 acaaagcgca taaaggctgt ctgcccttgt tctgtctgct tgtagactcc tgcatcctca
    16741 agtacacgca caaagatagc acccacagag tccttgacga ttgcaagggc tttttcttta
    16801 tccgttaggt ctggatgttg ggatttgagt tggtctgccc actcctgatg ataatcggca
    16861 actgtaacag cttctcctac aagatagcta gcgacttgct ccacttcttc tttcagacgt
    16921 ggtggcaaga ttgccaagcc catgacctca atcaagccga tattttcctt cttgatatgt
    16981 tggacatcct tgtggggatg atagatgcca tcaggatgct ctgctgaagt ctgattgtct
    17041 cgcaagacca agtccaactc aaactgtcca tcacacttac gcgctattgg tgtgatggtg
    17101 tgatgcggcg tcccgtctgt ctctgctaaa atctgcacac tagaatctga atactgacgc
    17161 cattcctgga aaatcttatc agctaagtta atcaaatcct ctttggaatc cgaagtcaag
    17221 cgtaggacag acattggcca cttgataatt ccagccttga cctgctcaaa accagcaaat
    17281 cggaaggtct tttgcaaggg agccaattcc ataggaaata cgtgacggcc tccctgataa
    17341 tgatcgtgag ttagaataga gccccccaca atcggcaggt cggcattaga tccagcaaaa
    17401 tatcctggaa actgctctac gatagccaac agacgttcaa aactctgacg actaatggcc
    17461 atgggacgat gctggccatc taaaaagata caatgctcat taaagtaagc atagggcgaa
    17521 tactggaacc cccattcctg accaaccatt tcaaaacgga taatccggtg gttgctgcga
    17581 gctgggtggt tgacccgacc atggtaaccc tcattctcta aacaaagttg acactgagga
    17641 taattactag cttgcaccaa cttggctacc gcaatctctt tcggatcctt ttcaggctta
    17701 gagagattga tggtaatttc aagttctcca taatcagatg gaacacgata agcgatattt
    17761 ttagcaatgg ccttgagttt gatgtagtca tttttctgac tgagttgata aaaatcctct
    17821 atcgcttgct caggagagta ggcataggct tcccagaagt cacgattgac ctgactcgga
    17881 caaggagtca ccaagtccat cagttcagta ccaaggattt cacgcgcagt ctgactatcc
    17941 tcaatcgtct ctaatcgaac ggcttcctca actagctggg ccttgaggtc aatcaattta
    18001 tccagattgg tctcaacttc caaaacacct tctcccactc gtgccaaaac acgattggtc
    18061 aggtagattc gatccatttc ctcaaatgaa ctttcagaaa tgacatgtgt tacaaattta
    18121 tctactaagg tcactataga gcctcctttt gactagtcaa ggacgcgagt gccacctgca
    18181 acttcagcga tatagaagct tggagcgtat ccaactactt cctcgtagtg tttgcctaca
    18241 gcttccttaa aggcctcaac agtatctttt tgaaccaagg caatggcaca gccaccaaaa
    18301 ccagcccctg tcatacgagc accgagaact ccttcttgtg cccaagctgt gtgaacaagg
    18361 gtatctaatt ccaaaccagt tacttcataa tcatgctcca gagaaacgtg tgacgcattc
    18421 atcaaacgac caaatgtttc caaatcacct gcttgaaggg ctgcttgagc tttaagggta
    18481 cgttggtttt caagcacagc atggcgagca cgtttcaaac gattttcatc tttaattaga
    18541 tagctatatt ggtcaacggc ccactcgtcc aattcaccca gggtctgaat atccaaggca
    18601 acttgcaatt cttccactgc tttttcacac tcagcacgac gttcattgta tttagagtcc
    18661 gccaattcac ggcgtttgtt ggtgttcatg ataacaacga cattgtcctt caaatcaagt
    18721 ggcaccaaat catactctaa ggtgttggta tcaaggtaaa tagcacgttg atcagccccc
    18781 ataccaatag caaactggtc catgatacca gagttgactc cgataaagtt gttttctgtt
    18841 tgtttgccga ttttaaccaa atcgagacgc tctaatttta aatcaaagag atgctctgcc
    18901 acgactcctg tcaagagctc caaggatgct gaagaagaca agccagcacc atttgggata
    18961 ttcccataaa cataaaaatc aaaacctttg tcaatcacgt gcccagcttc ttgcaagaaa
    19021 tgaagaaccc cttttggata attggtccag ttgtgctctt tttcaaactt gaggtcagcg
    19081 agaggcactt cgataatgcc cttgtcctca aagttagctg agtagaaacg caagacttgg
    19141 tcgtcacgct tacgagctgc accgtaagtt cccaaggaaa tagcagcagg aaaaacgtgc
    19201 ccaccgttgt agtctgtgtg ttcaccaatc aaattaatac gacctggtga aaagaaggtt
    19261 tggtctgctt cttgaccaaa aacagcaaga aagtctttgc gaaggacttc agtagtaaga
    19321 tgttgtgcca tatgaattct cctttgactg tccgttaagc catcttaacg tgtaatcgtt
    19381 ttcttctatt gtatccaagg aatttcccaa ggtcaatcct ttttactaaa attttagtaa
    19441 aacatcagta aaaagaagaa aaatatgata tactaaccta atcaaggagg atttatggct
    19501 accttaaaag acattgcaca gctagcctct gtctctatcg cgaccgtatc ccgtgtcctc
    19561 aaccgcgacc agagcctatc tgttacagaa gaaaccagac accgtatttt aaccgttgct
    19621 gaagagctgg gctacaccaa gcacctcaag acaggcgagt cccataaacc caagcaaaag
    19681 attgccatta ttcaatgggt cagcgaacaa ggggagctag acgacctcta ctactaccag
    19741 attcgcctag gaatagaaaa aagagcccaa gagttggact acgatatctt gcgctatttt
    19801 aatgaccatc cttttaccct aagcgaggaa gtgattggga ttctctgcat cggaaaattt
    19861 agccgagctc agatttctgc ctttgaagaa taccaaaagc ctcttgtatt tctagacagc
    19921 gatacacttt ccctgggaca tacctgtatt atcacggatt tttacactgc tatgaaacag
    19981 gttgtcgatt atttcctcag tcaagggatg gaccgcatcg ggattctaac agggcttgag
    20041 gaaacaacag accaagaaga aatcattcag gacaagcgtc tagaaaactt caaaaactac
    20101 agtcaagcga ggggaatcta tcatgatgaa ctggtctttc aaggaagatt tactgcccag
    20161 tctggctatg acttaatgaa ggaggccatt cagagcttgg gagaccaact tccgccagca
    20221 tttttcgcag ccagcgatag tttagctatc ggtgccctcc gtgccctcca agaagctgga
    20281 atcagcctgc cagatcgcgt cagcctcatt tcattcaacg acaccagtct gaccaaacag
    20341 gtctatcctc ccctctctag tattacagtt tatactgaag aaatggggcg agcaggtatg
    20401 gatattctta acaaggaagt cctccacggt cgcaaaattc ctagcctgac catgctggga
    20461 accagactga cattaagaga aagtacccta aatcaagaat aggataacat aaaaaacgaa
    20521 tagagttcta aaactcctat tcgtttttta ttcgattaca atcatagact taattgtctt
    20581 acgttcatcc atatctttat aggcttggtc gatatcttcc agtttataac ttgaagtaaa
    20641 gacgcgacct ggattgatat caccatcaag gacggctttt agtaaaaatt gcttatcgta
    20701 tgttgtagca gaagctgccc cacctgctac agagatattt tgcataaatg tcgaaccaag
    20761 agcacgatta ttatagtgtg ggactcctac aaagcccata cgccctccat tatgaagaac
    20821 acctagcgcc tgttctatag cagcctccgt accaacacat tcaagtgctg cgtctgctcc
    20881 tccgccgagg atttcacgca ccttggtaat tccttcttga ccacgttctg caacaacagc
    20941 tgtcgcacct gactccatag ccatcttttg acggtcttca tgacggctca taaggataat
    21001 ttgtgatgct ccacgcatct tagccgcgat gacagcacat tgaccaacag ccccatcacc
    21061 gataacaaca accttgtccc ctttttgaac atttgcaaca cgcgctgcat gatagcctgt
    21121 cggcatgaca tctgcaagag tcaaaaggga cttgagcatc ccttctgtat aatcagaagg
    21181 ttgaccaggg attttaacca gcgcccagtt tgcatagtgg aagcgaatat attctgcctg
    21241 aaaatcaccc cccaaattat taccaatatg attgtcgcaa gaaccgtcaa atccagcaag
    21301 acaggcatca cactcaccac atccatgtgt aaaagggaca atcacaaaat cacctggttt
    21361 caccgtcgta atggcttccc cagcttcttc aacaatccca atcgcttcgt gtccactatt
    21421 tttatgtcca gctttcgttt ctggattacg gtacctccat aaatctgaac cacaaacgca
    21481 cgcacgaacc acacgaataa tcacatcatc cgcttctatt atttgcggac gttcaatgct
    21541 agcaagtcca acctgacctg cctttgtata tactgctgat ttcatttaaa attttccttc
    21601 cttataaagt ttaattttga gatttaaacg atttaaagct gtctgtagct gagacaagcg
    21661 ctcctctaat ttttgctttt cctcttctaa aataccaagc ctctcctctc tcgtttcatc
    21721 tcccttttgg tagagcgaca tatagtcaac taaactatct acagagacac ccgccgaacg
    21781 aaaacactta ataaattcca gcgcttcgat atcctgatct tgaaaatcac gaatcccagt
    21841 agcagtacga gtaatcggtg gcacaagacc aacccgttca taataccgaa tcgtatccgc
    21901 tgaaattccc aacaaatcac tggcagattt aatattcaca cgctaacctc ctctcatttc
    21961 attcactgaa ttcacgctat tctaactcac ttatcttatt atataacttt gagttgactc
    22021 caagtcaagc attttgtatc taatcgaaaa atttttatca ttaacaatca tatatctgga
    22081 gtttatacaa tgctcttctc ttaccaattt ttgaaatatt taaataataa agaaattact
    22141 ctattccaaa taagatagta cccaagtatt tttcactttt ttctaatgtt gatgctcata
    22201 actccgttcc aagtcacaca cctttcgctt atgggtttgg tgagtttcta ggtcagcatc
    22261 aatttcaatg gtaatatttt gaaaaccaca atcttttagg aaaattcgaa tagactcttt
    22321 acaagtttcc atatgttcca tttcttttag acaaacatgt acaatggcat ttttttccaa
    22381 agcatccata gtccagagat taagctgatt aaggctggcc acattgtcca atcgctccag
    22441 gccactcttt acttgcttga tatcaagacc ttctggcaca gcatccaaga aaatcttgag
    22501 tgtagaccaa aaacgtggaa gggcttttga aagaataaag aaagaaatga caagggacaa
    22561 aagaggatct aggatatacc agtccgtaaa tcgaagaaca atcgccatca ggataacagc
    22621 tacccaccct agcgtatctt ccagaaaatg caaactcaga atagactcat tctttgtctt
    22681 tcccttacca accaccagac tcgctaacag attgatagta atcgcaataa ttcctaacca
    22741 gagaatcccc tcatcattga ctggttgcgg atgcaaaatc ttcgtgacat tttccaaaat
    22801 gactagaaca gagcccgtta cgagaatcac agctgttacc aaggctccta gcaggctaaa
    22861 ccgcttatag cccaaggtgt actgattgtc ttcttcacga ttggagattg tttctagaaa
    22921 agctgatatt ccaattgcaa tcgcatctcc caagtcatgc acagagtcag caagaacagc
    22981 gctagaacca aatactccac ctgcaataaa ctcaacaatg gcataagtca aatttaagaa
    23041 aaaagccacc caaacagcat attttgcctt catatttctc attcctttgt tataatagat
    23101 ttatgaacac cttgttcatt atcattatcc actaaaactc aaagaaagga tagaagcaaa
    23161 acgtcagctt tattcagttc tgaacaattt gccttaagtg tccatatgac taacattgac
    23221 cgccgtatca gcaaaaccaa aaaagccatc tatcaagctt ttatacaact tttgaatgct
    23281 aagggctacg aggccactac tgttcaggat atcattgatc tcgcagatgt gggaagatcc
    23341 accttttact gtcactatga aagtaaggag cttcttctgg accagctctg ccgctacctc
    23401 ttccatcatc tttttgaaag agagcaagcc atttcaaccg aggactacct cgcccacctc
    23461 tttctccatt ttcataaaaa ccaagaccat atcaccagtc tgctattttc caaaaatgac
    23521 tacttcctcc gtcaactcca taaagagcta gaacaccatg tctattccgt gctagctgat
    23581 aatttaaaag aagcccaccc taatatgcct acttcttacc tccaacactt ggtcgtgtcc
    23641 aactttatcg agacattgac ctggtggctc aaaaaaggtc aagatttcac aggccaggaa
    23701 gttgtccaat tttatctaga ccttctcatt cctaaaaatt gaatatagag taaagctcag
    23761 ttgtcttatt tctaggttac tgagtttttt atcttttcaa caacaaaaga ggacccgccg
    23821 atcctctttt tcatactata aatccttgat tatcaactat atctgtttta atcgaaatct
    23881 caaaacagca ctttcaaaca tcttttccta gttaagtaaa tcagtattct gcttagctgc
    23941 cttgctccat tgataccaac caactagact gttaatgaga taaattagat atttcccttg
    24001 aatttgcagg ctttctcccc accagagata gattgaaaaa acattggtag ccgcccagaa
    24061 tatccactgt tcacggtaaa cagctgtcat gaggatttgc cctaccccat tggttgcatc
    24121 tgtgattgaa tcacgatagg gacgattggc accaatagac tgataaatga agccaaaggc
    24181 caaccaccaa agcacactaa tggaaagata ctttgtccag cccttgccgt ccagtttacg
    24241 cgcgacaaac tcctgctttt ccttcttaaa ctgtgcctga taaatccaaa ctagaagtcc
    24301 aattggctgc atgactgtga agtaaagtgt cgtcagcacc tcaccataaa agcctttctg
    24361 tagggccaaa ataaggtaaa taacagagtt aatcaagcca aaaagataat tacttgctcg
    24421 accttccgat acaaagatta cacagataat ccctgtcaag ctacaaatca tcccaatcca
    24481 gtcaacaata cgatgttcgt aaaccaactc cagccagaga ggaaaacttc ctaaaaccag
    24541 caaataaatc cactgggcaa aactacgatg ggcaaagagg tcatcccaga tagccttcat
    24601 agttcctgaa aatcctaaat cagccatagc cgcaaccata cgacggtaac cacctgacat
    24661 ttcacctagg gttgttttga tattttcaat tttcttttgc aaataagtat gcatcatttc
    24721 tccttttgtt tttaaagagc cgtgtctgga tagactttcg gacgcaacgc tctattagat
    24781 aatgaactgc ctatacacaa gatttctaac cttagtcgac atgagctaaa acctcttatt
    24841 tgttaagtag ttcacaaaat attatacacc tattttatga atagtcaact gtctttacag
    24901 taaaatttta gaaaatcatg aaaattttct ctttctttcc attttaagtg acattcagtc
    24961 attctcacat caaaaaagcc cagacgaaat tgtctgagca ttcttttatc tagtcgttta
    25021 aggaagttga gttcagtatg tttaaagtct ctgtcccatc atttcttcaa caaaccttgt
    25081 tcttggagaa actccttggc tacttgcttt gctgacttgc cttcaacacc gacttggtag
    25141 ttgagctggc tcatctggct ttctgtaatc ttaccagcca atgtattaag aactctttcc
    25201 aactctgggt gtttcttgag aagagcttct ttcatgagtg gagccccttg ataaggtggg
    25261 aagagttgct tgtcatcttc caagacctgt aaatcataac gctccaattc cgcatcagtc
    25321 gaataggcat ccgtgatttg aatatcccct gactgaattg cctgataacg aagagctggc
    25381 tgcatagtcg ccacattgag attgagacca tacattgatt gcaagccctt atttccatct
    25441 tcacggtcgt taaactcaag cgtaaagcct gccttcagct gcccttccac ttttttcaaa
    25501 tccgaaatgg tcttcaagcc atattcttga gcaatctttt tcggaacagc tacagcatag
    25561 gtattttgat aagacatagg tttgagatag gctagatgat cctgcttggc aatgccatca
    25621 cgcgccactt gataaacctg ctctggctca tgactcacct tgggtgatgg ttgaagcaaa
    25681 ctttcagtca ccgtaccggt aaattcagga taaatgtcaa tatcaccttt tttcagagct
    25741 tcataaagga aacttgtcgt cccaaaattc ggtttaacag tcgcagtcat gctggtattt
    25801 tcttcaatca gcaacttata catattagcc aaaatttctg gttctgggcc tattttccca
    25861 gcaataatca agttttcctt ctctttttga gccaaaagag ctggactata agacagaccc
    25921 agtaataaag ccaccaaggc aaaaccagag aaaatcgtcc gcaatttcgc tttttccatt
    25981 acttttagta ggaagttaaa ggcaatagct agcactgcag aagaaagggc cccaatcaaa
    26041 atcaaactgg cattattacg gtcaattccc aacaggataa aggaacccag tccccctgcc
    26101 ccaatcaagg tcgccaaggt agccgtaccg ataatcaaga ccgctgccgt ccgaattcca
    26161 gacatgataa caggcatggc aagtggaatt tcaaacttct tgagacgctc ccatctggtc
    26221 atcccaaagg caatcccagc ctcttgaaga ctcggatcaa ttcccttcag cccagtaata
    26281 gtattttgta aaatcggaaa aatcgcataa atcactagag ctgtcaaagc cggcaaggtc
    26341 ccaattccca tcaaagggat aaagagcccc aacaaggcca gagacgggat ggtctggaaa
    26401 atacctgcaa tctgcaagac ccagtcggcc agcttctcat gatagcgaag aaaaacagcc
    26461 aagggaatcg caagcaaaat agctagtaac aaggtcaaaa gtgacaactg caaatgttga
    26521 gatagagctg tcaaccaatc actaaaacga tcctgaaaag ttgcaattaa attagtcatg
    26581 aacactacct ccaaacaagt ctgctacaaa gtctgttgca ggcactttta aaattgtctc
    26641 gggattcgct acctggcgaa tttctccatc ctgcaagaca gcaatacggt ccgccaactt
    26701 caaggcttca tccgtatcat gggttacaaa aatcgttgtc atcccaaact ctttatgcaa
    26761 ttcttttgtc agaacctgca actgttttct cgaaatagca tccaaggccg aaaagggttc
    26821 atccatgagg aaaatcttgg gctgaccaat catagctcgg acaataccga cccgttgctg
    26881 ttctccacca gataattcac taggtaagcg atgcccatac tcggctactg gtaaaccaac
    26941 cttagccaaa agctcttctg ttttcttcgt aatttcttcc ttgctccacc ccttcatttc
    27001 aggaatgaga gcaatatttt ccgcaactgt tagatttgga aaaagagcaa tagcctgtaa
    27061 aacataacca gtagaaagac gaagttcacg ctcatcatag tctttgatgc gcttcccatc
    27121 catataaata tttccatcag ttggttccaa aagacggtta atcatcttga gcatggtcgt
    27181 cttacctgac ccagaaggcc ctactaaaac cataaattcc ccatcctcaa tctgtaagtt
    27241 gacatctctc aagacatcct tttctgtgta gcgcagtgct acatttttgt attcaatcat
    27301 tctttgtcct caatttaaaa cttccctcga ttagtcaagt cttctacctt aggcatgact
    27361 tccttattat cccaatgctc cacaattttc ccgttctcta aacggaagat atcgtactgg
    27421 gcataagcaa cgccatcaat ctgagtctga ccatagctaa ccacatagtt tccttgtcct
    27481 aagagttgga aaacaaagtc aaaagtgaca ctatattcag ccacatagtt tttataagca
    27541 gcacttcctt gtccaatctc atgattatgc tgaatcaaat cgtctgccac ataatcactc
    27601 cactgctcta gctccccatt ttggaaagtt tctgtcaaga aacggcgaac cagcttttta
    27661 ttttctgctt tcttgtccaa atccttgatt tcaaaatctc caaaaatttg atctagttgg
    27721 tcattttcag gtgttcgata gtagtcaatg acatcccaat gctcaacaat acgaccattc
    27781 tcatccgcac ggaaagtatc cgtcgtcacc cattgagctt ctccaccatt cagatattga
    27841 tgaacatgaa caaagaccag attgccatcc tcaatggtgc ggacaatctt aatctgacgc
    27901 tctggatgac gctcaaagaa atctgcaaag aaggctgcaa atccttcttt cccgtcagga
    27961 acacctgtcg aatgttggat ataggtatcc cctacagact gggcttgagc ctcagcaact
    28021 cgtccgtctt gaatggcatg gatgtatagg ttgtgagcat ttttcacttg ttgtgacata
    28081 ttctaaacct cattttcctt ctctttcaga tttgccaaaa ttctttcttg aaaaccttca
    28141 aattggtgaa tttcttcctc tgaaaatcct ttgtaaaaga tagtatccaa tttctgactg
    28201 acacgatgcc ccacttcttt ctggaacttg cctaactccg ttaaaactaa atacttctta
    28261 cgcttgtctt ttccacacgg actaacaatt acaagctttt gttcctctag cttttttatc
    28321 atagtcgtca gcgtattatt cgcaagccca gtcgcaagcg cgatatctgt cgcagttgcg
    28381 cagccagttt cactattcca taaaaccgct aaaatcttgc cctgttcacc cctataaaga
    28441 gcctcaggat cttgactcag taacttttga aaaatccgcc cattcaacaa acgaatatga
    28501 tgggctagca aatgactatc tttcataaca cctccaattt atttcgatat cgaaatgaat
    28561 aaaataattg taacactcat cgttctaact gtcaactatt tcgatttgga aataattttt
    28621 gataattatt cacaccacca tactccggct caactaactt ttaacgagag tttctaaatt
    28681 ccttcgtcct ccagtctaca aaagccttcc atccgtacta tcctattttt tatgagggga
    28741 cacatttttc ctatcagatc atttatttta aagatagaag taaatcataa ttgcttccat
    28801 ctgttctttt atagtatatt gaagttggac tagggcactg tatcttctaa aacattgata
    28861 gaaagcgatt tgaatttccc aatcaatttg ttcgtattta tagcattttg aaactagaat
    28921 aggacgccat gactgctaaa agatttctat aaattcattt gatttcccta atcaatttgt
    28981 tcatatctta tttcattccg ctataatttc accttaccct atctttttcg tagcaccctt
    29041 caaacagcct atcccctacc gtttgatgat tcctcacttc actccacttc cattacagaa
    29101 gtttcttcac tactatggtc tcggctgact tctcatgatt ccttgttact actatttgaa
    29161 cgctcacgag ataggtctta taaaaaatgc tttgatccac aatggaatca aagcatttta
    29221 aaaagttcct catacataag cgcagaagtc gcagttcctc tgtacttggc ttcttctctt
    29281 ttgacaaagc gagccaagtt gagcaactca ggtgctggat gtttgggatt taggagcaat
    29341 tcacgattga ccaggcttga gagacgaact gcctgcaatt gctcatttgt agtaggcagt
    29401 tttttagtag tctctaggag agcagcaact aaatcttcac tcaaatcatg tcgagcatga
    29461 ttgtaaagat cttttataag gctttctagg tttggttcta ccatccctac cacctccctt
    29521 atggtttaat aatgtttaat caaatcaacc gttgaacgat ccaatttctt caccaaggct
    29581 tgtaagaaag cttgcgcttc taggaagtca tccattgcat agagggtttg gtgagaatgg
    29641 atataacgag cgcagacacc gatagttgtt gatgggacac caccattttt cagatgagct
    29701 gcacctgcat ctgttccgcc tttaccacag tagtattggt acttgatacc agcttcttca
    29761 gccgttgtca aaaggaaatc cttcatccct gggagaagca agtgacctgg atcatagaaa
    29821 cgaatcaagg ttccatctcc aatcttgcct tgaccaccgt agacatcacc tgctggtgag
    29881 caatcaactg cgaggaagac ttctgggtca aacttggttg tagaggtatg agcgccacgc
    29941 agaccaactt cttcttggac gttagaaccc agatagagtt cattgccgag tttttgacct
    30001 gataaagctt cagctagctc gcttaccatg aggacaccgt agcggttatc ccaagctttt
    30061 gagatgatat ttttttcatt ggctgtcaaa attgcagaac tatctggtac gatggtatca
    30121 ccaggacgga tgccaaaact ttctgcctca gccttgtccg caaaaccacc atcaaaaacg
    30181 atatcggcaa tggctggcat ggttggtccc ccctttccac gagtcaaatg cggaggaaca
    30241 gaacctgaaa tcacaggaat ttcatgacca tcacgagtca agagtttgaa acgttggctg
    30301 ctaaccacca tggggttcca gccaccgatt tctacgacac ggaaggtacc atctggcttg
    30361 atttcgctga ccataaaacc aacttcgtcc atatgagaag cgaccaagac gcgcggtgca
    30421 tccacagctt ctgaatgttt gataccaaaa ataccaccca agccatctgt caccacttca
    30481 tccacatgcg gtgtcaactt ttcacgaaga taagcacgga caggcgcttc atgacctgag
    30541 actgcagcaa gttctgttac ttctttaatt tttgaaaata atgttgtcat ttcagttcct
    30601 tctttctttc atccatttta ccacttttta taggagaagg atagtgggaa ggtggatttc
    30661 taagttagta tcttagtcct gctctatctt agaaaaggat agtattctct tgcatgtagt
    30721 gcaaaatcta gtaaacattc caaaattaac tcgaatattt atttccaaac aaaaaaacaa
    30781 tacaccatca aagttgtttg gatttttcat gaaatttaca gaaaatagtt gacttccctt
    30841 tcttctttct ttaaatatat agttggttga gtttggaata gtacgctgta gctgctgaaa
    30901 catttctaga aattaatttg actttcctaa tagagttgtt catatcttat ttcaatttac
    30961 tatagtacaa aactagaaaa ggaaaaaatc atgaccaaaa caattcttgt tacaggtggg
    31021 actagctaca ttggctccca taccgttaaa gctcttttaa atgctggcta taaggtgcat
    31081 ggcctcgata atctctccac aggaaatcga gcggctgtgg atagtcgtgc tagctttaaa
    31141 gaactggatg tttatgatgc tagcgcctta aaggcttact tagaagaaaa tcagattgat
    31201 gctgtcctcc attgtgcagg tgaaattgtt gtgagcgaaa gtattgaaaa tccaagtaaa
    31261 tacttcactg ccaacgttgc tggtatgaac caagttctca aagtcttatc tgaagttggc
    31321 attcaaaaaa tcatgttctc ttcgactgct tctctctatg gaaataactg tattgacaag
    31381 ccggtgactg aagataccct gctcgaccct gtcaaccctt atgcagagac aaaactgatg
    31441 ggcgaacgaa tgatttactg gatggccaat cgctacgact ggaaatatgt tattttccgt
    31501 tactttaatg ttgctggggc tgaaatggat gcttcaaacg gtctgcgtgt gaaaaatcca
    31561 actcacatca ttccaaatat caacaagacc gcattgggac aacatgatag cttaaaaata
    31621 tttggagatg actacgatac acgtgacggt tcatgtattc gagattacat ttatgtcttg
    31681 gaccttgcat aggctcatgt taaaggaatg aactacctat ttcaagaaga cagttcttct
    31741 caaatcttta acttaggaac taaaaaagcc tatactatca aagaaatctt taaaactgct
    31801 gaagaattac taaattaaaa aatcccacac gagattgttg ctcgacgtgc tagtgaccca
    31861 gccagtgtcc tagcagatgc ttcaaaagct aaacaatatc ttgattggac ggctagctac
    31921 tccctcgaag atattatttt atcagattaa ggtaaaaaca ttttagaata ggataacaaa
    31981 ggagaaagtc ttgttgctct ctccttgttg tatttattaa attgtcagaa attttattga
    32041 cagattagaa aaataatgtt acatttatat ccgcagatat ctttcgatac caaatctaca
    32101 tgaaaggacg gggtatgaaa ctttctcatt atttaattgg cttacttcta ctcctagtct
    32161 ttctctctat tagcattggg accagtgatt tttcatgggg aaagctattt gatttcgacc
    32221 agcagacctg gctcctcttt caagagtccc gtctcccaag aactatcagt attctcctga
    32281 ctgcctctag tatgagtatg gcaggccttc tcatgcagac tattacccaa aatcagtttg
    32341 ctgcaccgag tacagttgga acgactgaag ccgccaaact gggaatggtg ctgagccttt
    32401 ttgtctttcc atcggctagt ctgacccaaa agatgctctt cgcttttgtt tcatccatcg
    32461 tattcaccct cttcttccta gcctttatga ccatttttac tgtaaaggaa aggtggatgt
    32521 tgcctctgat tgggatcatc tatagcggga ttatcggctc agtcacagaa gttatcgcct
    32581 atcgtttcaa tctggttcag agtatgactg cctggaccca gggctccttc tccatgattc
    32641 agacccatca gtatgagtgg ctcttcttag gcctcatcat cctgataacc gtttggaaat
    32701 tatcccaaac cttcaccatc atgaatctag ggaaagaaac cagcgagagt ttggggattt
    32761 cctactccct acttgaaaaa ctggccctct ttctggtggc gctaacgaca agcgtcacca
    32821 tgattaccgt gggtggccta ccatttctcg gagttatcgt tcccaatctt gttcgcaagc
    32881 gctatggaga taatctaagt caaaccaaac tcatggtcgc actggttggt gccaatctag
    32941 ttctggcttg cgatatccta tcccgagttc tgattaggcc ctatgagttg tctgtcagtc
    33001 tcttgctagg aatcatcggt agtctcgtct ttatcctact tctctggaga gggggacgaa
    33061 aagatgcaga ctaaaagcaa acataccaag ctcttctgga ttctcattat tcttgccatc
    33121 ggagcttgtc ttctctactt ttggcccatc actcacttgt cagcctttgc ttggaagttg
    33181 cgttcccaaa agatcatcgt ttatctcttg gtagccatcg cgactgggat ttcgaccatt
    33241 agttttcaaa ccctgacgga aaatcgcttc ctgacgccta gtattttggg aattgaatcc
    33301 ttctacatcc tactacaaac cctactactg gtttttgaaa gcaagtttct tcaacttggc
    33361 aaatccccta tcttagaatt cctagtctta cttcttgtcc agtccctctt ctttctcgcc
    33421 ttacaaggtt acttgaagac actgatgaag caagacctgg tcttcatcct gctgatctgt
    33481 ctagcgctca gaagtctctt tcgaaatatc agcaccttcc ttcaagtcct aatggatcca
    33541 aacgaatacg ataaactgca aaatagtctt tttgcctcct ttcaacatct caacacttcc
    33601 atcctagcca tcggttctct gatcatcctc gctttgacaa tctttttctt tcgaaaagca
    33661 gtcgttctag atgtcttgca cctgcaaaga gaaacggctc agatattggg actcgatgtt
    33721 gaaaaagaac agaaagagct cctctgggga atcgtgcttt tgacctcaac ggccactgcc
    33781 ttggtaggac ctatggcctt cttcggcttt atgctggcca acctcaccta cctgattgtc
    33841 aaagactatc agcacaagtt actctttata gtggccattc tgattggatt tattagctta
    33901 accttggggc aagccttgat tgaacgagtc tttgcactgg aaattcgtat cagtatgatc
    33961 attgagagtg tgggtggctt cttattcttt atcttactat ataggaggtc tcgtcagtga
    34021 aactggaaaa cattgacaaa tccattcaaa aacaggatat tttgcaaggc atttcgcttg
    34081 aagtcagtcc tcaaaaactg acagccttta ttggtccaaa tggtgctgga aaatcgactc
    34141 tcctctccat catgagcaga ctaaccaaga aagatcaggg agttctcagt atcaaaggac
    34201 gtgaaatcga gagctggaat tcgcaagaac tggcccaaga actaaccatc ctaaaacaga
    34261 aaatcaatta ccaagccaaa ttgactgttg aagaactggt cagttttgga cgttttccct
    34321 acagccgagg tcgacttaga tcagaagact gggaaaaaat ccgagaaact ctgaactatt
    34381 tggaactgac caacttaaaa gaccgctaca tcaatagcct gtcagggggg caactccagc
    34441 gcgtctttat cgctatggta ctggcccagg atacggactt tatcttgctg gacgaaccac
    34501 tcaacaatct cgatatcaag caaagcgtca gcatgatgca gattcttcga cgactggtgg
    34561 aggaactcgg caagaccatt atcatcgtcc tccacgatat caacatggcc agtcagtatg
    34621 cagatgaaat tgtcgccttc aaggacggcc aggtctttag caagggaaga accgatcaaa
    34681 tcatgcaggc tgacctactc agtcaacttt atgagattcc catcacgcta gctgatatca
    34741 atgacaaaaa gatctgtatc tatagctagt aacataaaag ctcaagttag agaaccttca
    34801 gtctcttagt caataagatt aagagactcc ctaaatcgtt atcacatttt aaaaaggaga
    34861 aattatgaaa acatccctta aactttattt cactgcccta gtggccagct tcttgctcct
    34921 acttggtgca tgtagtacaa actcaagcac tagtcagaca gagaccagta gctctgctcc
    34981 aacagagata accattaaaa gttcactgga cgaggtcaaa ctttccaaag ttcctgaaaa
    35041 gattgtgacc tttgacctcg gcgctgcgga tactattcgc gctttaggat ttgaaaaaaa
    35101 tatcgtcgga atgcctacaa aaactgttcc gacttatcta aaagacctag tgggaactgt
    35161 caaaaatgtt ggttctatga aagaacctga tttagaagct atcgccgccc ttgagcctga
    35221 tttgattatc gcttcaccac gtacacaaaa attcgtagac aaattcaaag aaatcgcccc
    35281 aaccgttctc ttccaagcaa gcaaggatga ctactggact tctaccaagg ctaatatcga
    35341 atccttagca agtgccttcg gcgaaactag tacacaaaaa gccaaggaag aattggccaa
    35401 gctagacaag agcatccaag aagtcgctac taaaaatgaa agctctgaca aaaaagccct
    35461 tgcgatcctc cttaatgaag gaaaaatggc agcctttggt gccaaatctc gtttctcttt
    35521 cttgtaccaa accttgaaat tcaaaccaac tgatacaaaa tttgaagact cacgccacgg
    35581 acaagaagtc agctttgaaa gtgtcaaaga aatcaaccct gacatcctct ttgtcatcaa
    35641 ccgtaccctt gccatcggtg gggacaactc tagcaacgac ggtgtcctag aaaatgccct
    35701 tatcgctgaa acacctgctg ctaaaaatgg taagattatc caactaacac cagacctctg
    35761 gtatctaagc ggaggcggac ttgaatcaac aaaactcatg attgaagaca tacaaaaagc
    35821 tttgaaataa gccacttcaa actcaattta atactcaatg aaaatcaaag agcaaactag
    35881 gaaactagcc gcaggttgct caaaacactg ttttgaggtt gcagatgaaa gctgacgcgg
    35941 tttgaaggga ttttcgaaga gtataaaatt ttgacattct aaaacgcatc ctatcaagtt
    36001 cactcaaacc tgataggatg cgtttttatc attttgaaat ctatttaccc cacctcattc
    36061 cccttcttga tttcgtttaa gggaaaagtg gtctggaaca gggtccttac ctgttttcga
    36121 ccagggatga caacgtaaaa tccgagccaa gcccatcaat actcccttaa aaccatgttt
    36181 ttcaatagcc tgaatcatgt agttggaaca agtcagatca aagcgacaag agggtggaaa
    36241 gactggtgag ataaaacgtt ggtaaaagcg cacaggcgct attaaaattc gtttcattat
    36301 ttcttagtta cagccatggt gtgtagttgg ctgatttctt ttttattaag acgacgggat
    36361 tctcctggac ggagtcctgt caagtctagg tgtccgaaac gagtccgaga caacttatct
    36421 acttggagac caacagcttc aaacatcttt ttaacctgat ggttacgccc ttcatggatg
    36481 gtcaactgca ccacagagcg atttttgact gggtccactt tgagaatttc ataaacagct
    36541 ggcttggttt tcttaccatc aatctcaaga ccacgggtca aggggcggag attgtcctta
    36601 ttggccacac ctttaacacg cgcgacataa accttgtcaa tctcattacg agggtgaatc
    36661 atctcgtctg taaagtcccc atcattggtc aaaatcaaga cacctgatgt atcccagtcc
    36721 aaacgaccca cagggtaaat acgctctttg acattgggca agaggtcgac aaccgtcttg
    36781 cgacccttat catctgtcac actggaaatc acaccgcgtg gtttgttaag cagatagtag
    36841 accttttctt cgttgtagat aggttgacct tcaacttcga ccttgtcgcc tgacttgata
    36901 gtggttgcta gttcacgcac cacttggccg ttaaccgtca ccaagccttg cttaatcagc
    36961 tcttctgctt ttctcctact ggccacacct gcgtgggcaa tatacttatt gattctcatc
    37021 ttcttctatc ctttcaccaa ataattggct ttcttgggct tgaatctcaa gctcatcaat
    37081 cactggtaat tcttctaaat ggtttatccc catgtaatct aggaaataat ccgtagtcac
    37141 atagaggttg gggcgcccca atacttcctt tttcccgtct tcctttatca ggtcaaaagc
    37201 ctgcaacttt gccaaggctc cactcgagtt aactccacgg atggcatcaa tttctatccg
    37261 cgtaatcggc tgtttgtagg caataatgga caaggtctca agggcagccc gagacaagct
    37321 ctggttgata ggcgccttag agtattcctt caaaatctct gcaaattgag gcttggtcac
    37381 caatctataa gcaccacttg tctcaatcaa agccaaactg gaatctgggt ccttttcata
    37441 cttctgggct aattttccta aactttgctg gatgcctgtc ggtggcagag agaggagttc
    37501 agctaactgg cggacccgaa tcccatcttc acccgctaca aacaagagcg cttctatttt
    37561 tgctaaagta ctcatctttc ctctctatca agtctagctt tgaggcactt gactttcttc
    37621 cttcttttcc atgagataga tatctccaaa actctcctct tgcacgagga tcagctcctg
    37681 ggttttgatt aactctaggg ttgccaaaaa gagggtgatg acctcttgga cattctgggc
    37741 ttccttgaac aaatcctgca agcgcaattg atctcgtcca atcaaggact ctttcacgat
    37801 aatcatcatg tcctcaatct tatactcatc ccgcaagatc gtcgtgtgat tttgtgcaaa
    37861 ctcctctttt ttcttggcta ggatatttga aaaagccaaa aagaggtcaa tggtcgtctt
    37921 gtcatgcaca agctccgcat cttcgtaaat caactctgtc ggcgctttgg aataatactg
    37981 ggcccgttct tggtgcttgg cttccaagtg ctcacccaag agcttgaact tgcgatattc
    38041 ttcgatttga gagaggaggt cctgctccag gtcatccccc aagtctgtca cttctgctac
    38101 cttcggaagg agtttacgac tcttaatcag catgagctga ctagccatga ccatgtactc
    38161 acccgtcact tccagacgca tggcctgcag ggttgagaca taggctagat actgttcgat
    38221 gacttccgta atgggcacat cgtagatatc catctggtac ttagaaacca gatgcaagag
    38281 caagtccagg ggtccttcaa aatcttttaa tttaatatcc attatctata tttttctaag
    38341 gtcaggactg tttttaatcc taattttttt gcaatttcgt acaaatcgac cttgttttct
    38401 atttgtctta gaataaactg ttcacgtaag acttgagctg ataaggatgg aaaacctttc
    38461 tccttgacaa aagattctaa ctgacgaaag gcccactgac gagaataggg tttctctcct
    38521 ctttcaaaaa gataggtctg ccccatcaag ggttccaatt ctgaaagcaa ggccgtggga
    38581 atggtgacaa tcctctgttg ggaagccttg ctgattcgca gcacctgaaa atccagattg
    38641 atgtccgcaa ccttgatggc taaaatctca ctgggcaaga gccccatttc taggattaag
    38701 agcgctagca agcggccctc tggatggtcg ctttcctgcc aaaaagagtc taggtataga
    38761 atctctggct tttccgtctt cttttcagct tgtttggcta attccaagcg gtaaaagctg
    38821 tccacctctc ctttttgata gagaaagtat agaaattggt tacaggccga aatctttcgc
    38881 ttctgggcgc tgatttttag attggctagc tgggcttggt aaatcttgag actggtctca
    38941 gaaatccgct cacctaccat gtctaaaaat tgctccaaat catacttata ggactgcttg
    39001 gaattgacag acaagccctg cttttcctct aaaaaggctg aaatcctgtc tctcattggc
    39061 atcgaatctc atattcctta ctaaagtcat gcaagagggc attaacggcc ttgaggatgg
    39121 acttgcgcgt aataatccct tggaaaatac cctctgcatc cacgaccggt aagaaggact
    39181 catctactag cttgtgcaag acctccgtaa tggtgaaatc aggcgaaaca accgctacgt
    39241 ccgtttttgt catatgaacg atatccgtat ccgccatgat ttcttggctc aagtcatgct
    39301 ccatctgata agccataata tctctgagtc caatcgtccc aacaaactgt ttttcatctg
    39361 tcacaacggg aacacgggta taggtcatct gactgagcaa gagggtcgca tgatccgcat
    39421 tgtgggtatc aatcaacaca gctagatttt tagcaggggt caaaaaagtt tcctcctgcc
    39481 ccaacaagaa agtctcaaac tccttggcaa tcatcggcta aactccttgg acaaacctgg
    39541 atacacctcg tgatctcgtg tcaaaaagtc cactttgaag taactatcat caatctccac
    39601 acgagcatag agacattctc tgatggtacc tcgtggttga ctgatagaac ctggatttag
    39661 aaagaggatc ttgccttcca accaagcact tggcacatgc aagtgaccat agaggcagat
    39721 agcggcctct tcctcctgag cccagtagtc caacttttga aagttgaaat tgatgtcaaa
    39781 caagtgacca tgagtttgga taatcttggt cgaaccaagc tcagtcacca gacgttctgg
    39841 gtagccggcg tagaagtcca tgttcccttt aacaacgcgg atgccctccc aaagtggaga
    39901 atccggacgt agttcagaat cgccgttatg aaaaacggca tcgactttgc ccacatagcg
    39961 atcacggact tcttccacaa tcaagctatc gccatgggaa tcgctcatta caatgatggt
    40021 ttgctttgcc atgatggaaa tacctccaaa agtttcttaa cggctaaggc acggtgagat
    40081 tgactatttt tttcttctag ggttaattca gctgatgact cacctgtctc tcctacaagg
    40141 aagaggggat catagccaaa gccattttca cccttaggtt caaagttaat ataacctgac
    40201 cagtctgctt caacaactaa actttcctta tttgggctgg ctacgactag ggttgtgtgg
    40261 aactgagccg agcggtcctt gagttcaaag accatggcca attcgtgcaa gagtttggca
    40321 ttattttcac ggtcagttgc tcccacacct gcgaaacgag ctgaccagac gcctggtaag
    40381 ccaccaagga catcgacttt gagaccagaa tcatctgcca aaaccatctt acccgttaat
    40441 tgagaaatgg tttctgcctt aaggcgggca ttttcttcaa aggtcatacc tgtttctgct
    40501 acttcaggca ggtcagggta gtcattaaga ttttccacat cgtagcctaa cttatcaaag
    40561 atagctcgga attccttggt tttgccttcg ttacgagtcg caatcaacaa ggtttctctg
    40621 accttgcttg tctcaaagaa atcatgaaca ttgacccctt ctttaggcaa ttctacatac
    40681 aagagttgcc cattttcaac caagagcagg gccccatgac gttgaataac ttccaagttt
    40741 cgtcccattt cttgattaag catatctacc acaaaggaga acaaacggta ggtagaacca
    40801 tagcgtaaaa cagtaactga aagcgggaaa ccatactctt catctccaaa tattttggag
    40861 aattcaaaca gaggaaaatc tgtcttatag tcgctcaaac tgttaacgcc accaaaaata
    40921 ctataagacc caacatacca gtcctggtca tccttatatt cataaatttt atttgtcata
    40981 attctacatg ctccacatga atctcttttt ccagccattc ttcaccaatt tgtgcaaaac
    41041 tttggctact ggctgttgtg taaaaacggt gatggagtgg tccagcatcg cgaccacgat
    41101 tgatttcaaa ataattgagt aagactgaga tatcccgtac gcactctgcc ccactatcga
    41161 tgagctgaac ctttggcccc atcacatttt ggataatagg gcgaaggagt ggataatgag
    41221 tacagcccaa aatcaggcta tccacctttc caaccaaggg acgcagggtt tcatagacca
    41281 ccttcttggt aacactggtt gacagggcac ctgactcaac caagggagca aacttgggac
    41341 aggccaagct ctccacctgt aagtcgggat ccagatcatg gattttctga cggtatatgt
    41401 ctgattgtac cgtcatgggc gttccaatca ctccgatttt cccaccttga ctggacttga
    41461 tggctgccga agctcctggc aaaattacac ccaagacagg aatatctagt tgagccttga
    41521 tttcttccca gacgaccgca gtcgcagtgt tacaagcaat gacaatcatt ttgacatcct
    41581 tggtcaagag aaagttgacc agctgccaag tatattcacg aatttgctca gcaggacggg
    41641 ggccataggg cgcccgcgcc gaatctccaa tatagacgat ttcttcatgg ggaagctggc
    41701 gcatgagctc gcgcacaacg gtcaagcccc cgacacccga atccaaaaaa ccaattggtc
    41761 gattatccat acagtcttct ttctagtctt ttttctgatt gatcgttcat tatgattcct
    41821 tgtcctgaac gaactgaccg acagcttgat agaatgtcaa ctacctctct cttaatcata
    41881 atcaaatatc aatagaatgc aaggaaaaag tctcatccct aaaccataac caacatagtg
    41941 agaagtctgg aactttcatc ccagactttt ccttatttat tttttctttt tattgttagc
    42001 aagggctgcc ttttgttggc ggatgatttg gtggtaaact tgttgtacct tagcttcgct
    42061 tggtttttga ccatttgcac tcaaaagagt acgaactgct tcagcattca aacgtgggtt
    42121 gtcagcaaat tctttttcga tttgcttacg aaccaagtac attcctccaa gagctcctcc
    42181 tagaaaagct agcacaatca atacaattgc taaaagtaaa tccatcattt ttctcctgtt
    42241 tctttttgag tccctttcat tataccacaa actagccacc ctgaccagtt tgttctacac
    42301 tttcagggat ggaatagaca gcaaaaagaa ccctgctttt ctgaaaaaga ggtctccttg
    42361 ctaagtttat actcaatgaa aatcaaagag caaactagga agctagccac aggttgctca
    42421 aagcaccgct ttgaggttgc agataaaact gacacggttt gaagagattt gcgaagagta
    42481 ttaatttaca taaatagcca gtgtttgata gggtttgagt agaattttct cagacacttc
    42541 tgcatcttca tagtttgata tcaaaatctg tccattttgg tagactgctg gcaagtcgat
    42601 ttccacttct ttagcataaa agttattgag cactagtaac ttttgatcct caaactggcg
    42661 ttcaaaagcg tagacttgtt tgctatcttc aaaggctggt ttgtaacttc cttctgaaat
    42721 gattggcatt tccttacgca gtcgaatcaa gtcttgatag aaggtaaaaa tcggaccctg
    42781 gatttcattt tctacattga tgtctttata ggatttacca gctttcaacc aaggagtgcc
    42841 tgttgaaaat cctgcatttt ccgaagcatc ccactgcatg ggaatgcgtg aattatcacg
    42901 cgacttagct tgaataatct ggaaggcttc ttgctgactc tttccttctt ctaagagcat
    42961 ctgataggca ttaagcgatt cgacatccac ataatcagcc atagaatcat agtctgggtc
    43021 aatcatcccg atttcctcac ccatgtagat ataaggtgtc ccacgtgaca ggtgaatgct
    43081 ggccgctagc atggtggctc cttccttgcg gaagttttga atatcgacaa aacggttcaa
    43141 ggcacgtggt tgatcgtgat tgttccaaaa gagggcactc caaccgtctt tatcactcat
    43201 ttccttaccc caactatggt aaagactctt caactcttca aaatcaaagg gagccaaggt
    43261 ccacttttgt ccatccttat agtccacctt gaggtgatga aaattaaagg tcatggataa
    43321 ttcctgacga tcaggcgacg aataaaggac acagttttcc atggtggtag aagacatttc
    43381 cccaactgtc ataaagctat cgtcagatcc aaaagtggct tggttcatca tacgcaaata
    43441 gttatgaacg atgggtttgt ctgtataagc tggcttccct tcattttcag gacagtccac
    43501 tgaaacctcg tccttaccga tcaaattgat cacatcaaat cgaaaacctt tgacaccctt
    43561 gtcgcgccag aaattaacaa ccttgaaaag ctccttacgg acattgggat tgcgccagtt
    43621 aaggtcagcc tgggtctcat caaataggtg aagatagtat ttcccagtat ccccgaaagg
    43681 cgcccatgca gaaccaccaa acttagactg ccaatctgtt ggttggtctt ggatgaagaa
    43741 aaagtcttga taatacttat caccagctag ggctttctga aaccattcat gctctgtcga
    43801 acaatgatta agtaccatgt ccagcataaa gtcaatcttg tgctctttac cgatacacac
    43861 catttcctca aaatcagcca tatcaccaaa aagaggatcc actgccatat aatctgaaat
    43921 atcgtaacca ttatcccgtt gaggacttgg atagaatgga ttgagccaga ccatatccac
    43981 acctagtttg gctagatagg gaatttttcc gataatccca cggaaatccc caataccgtt
    44041 ttcagtggtg tctttgtaag attttggata gatttgatag actacttttc ctttatcaag
    44101 tgtcatctgt ttctcctttt ctgataaaag ggaggaagca gtcttccgtc cctatttgtg
    44161 ctatttcaat tatactcaat gaaaatcaaa gaacaaacta ggaagctagc cacaggttgc
    44221 tcaaaacact attttgaggt tgcagataga gctgacgtgg tttgaagaga ttttcgaaga
    44281 gtattagatt cgtgtagcga ccatgagaga tgctccagct tggatcgttg tcggataagt
    44341 tccgggaata gtcgctgtat aagcatcttg gttggtgatg ataacaagag tttctgtcac
    44401 cagacctgca gccttaatga catccatatc aaaacgaatc agttgctggc caactataac
    44461 gtgatctcct tggactacaa gactttcaaa acctttgcca tcaagaccta ctgtatccat
    44521 accgatgtgg atgagcaatt caactccctc gtcagagaca atgccgatgg catgcttggt
    44581 agggaaaaga accgtcactg ttccattaac tggagaggtc aactcacctt ggcttggttc
    44641 aatgaccaga ccttgcccca tgacacctga tgcaaaaaca ggatccgtcg cttgactcaa
    44701 ttctttcact tggccagtta gtgggctgat aatttctacc gaagtaagtt ctactagttc
    44761 atggctcaca aattctgctt cttcttgagc aacgaattct gcctgcaagt tcgtatcgcc
    44821 ctctgttttt gtaaagagac cagccttgcg gaagaagaaa gtcaagagca ttggaacaac
    44881 aatcgcaact agcatagttc ctgcaaatgg cagcatgtat tgaggttgaa tagagagaat
    44941 acctggcaaa ccaccgatac caatagaagc cgcagttaca ttaaaagtaa cggataacat
    45001 gcctgcaagg gctgaaccag tcatcccagc aacaaatgga taaatatatt ttacgttaac
    45061 cccaaaaaga gctggttctg taacaccgag ataggctgaa atggttgcag gaagtgaaac
    45121 ctgagcctca cgctcatcat ggcgatgcat gaaataatag gcaaacacgg ctgagccttg
    45181 agcaatatta gaaagagcaa tcataggcca gagggcagtg ccaccagcat ccgcaatcaa
    45241 ttgtgtatca atggcattgg tcatgtggtg cagacctgtg atgacaaatg gagcgtagag
    45301 ggcaccaaaa attgcaccga agagccattt tacaggaccc gttaaacctg ccaatacaac
    45361 tgatgaaagt ccttgtccaa ttgtccaacc gattggcccc aagacagtat gagccaaaat
    45421 caaggctgga atcaatgaca aaaaaggtac aaaaatcatg gaaatgactt ctgggatatg
    45481 tttgcgccaa aaaatttcaa gataagacag actcaaacct gcaagcaagg ctgggataac
    45541 ttgggcttgg taaccaatac gattaacagt aaaataacca aaattccaaa cccagtttgc
    45601 tgcgatatca gctgctggcg ttgaagcaac cgcataggca ttgagcaact gaggcgatac
    45661 caaacagatt ccgagaacaa ttcccaaaat ttggctggtt cccatcttac gagaaacaga
    45721 ccaagtaatc cctactggta agaactggaa gatagcttca ccaggcaacc agaggaagtg
    45781 attaacccct gcccaaaact gagaggattc tgtgatggtc ttgccatcca acatcgacca
    45841 atggacacct tccaagacat tacggaaacc gaggatcaat ccaccaacaa tcaaggctgg
    45901 aataatcgga gtaaagattt ccgccagagt ggtcataaca ccttggacca cgttttgatt
    45961 actcttagct gcagacttgg ctgcttcttt ggaaacaccc tcaatacctg aaacggctgt
    46021 aaaatcatta taaaagatgg gcacgtcatt tccaatgatt acctgaaatt gacctgcatt
    46081 tgtaaaggtt cctttaacag ctggaattga ctcgatagct ttaacattag ccttcttatc
    46141 atctcctaaa acaaaccgca tccgtgtcgc acagtgggtg acggcagtca cattctcttt
    46201 acctccaatt gcctgaagca aatctttggc ttcttgttca aattttccca tttgattctc
    46261 cttatacttg tcaaagcaag tttgatttga taagtatatt gtaaacgata ccaaacttgt
    46321 ctgcaacttg tttggtataa aaagggtgtt tttttgtaaa ttttctagca cttgtatggt
    46381 aaaatagata caggtgttca ttaaactaga ctaaaaacct atttaagaaa gcaaaatgaa
    46441 aaaataccaa caattattta agcaaatcca agaaaccatt caaaacgaga cttacgctgt
    46501 cggagatttt cttcctagcg agcacgacct tatggagcaa tatcaagtga gtcgtgatac
    46561 cgtccgaaag gccctgtctc tcctccaaga ggaaggattg atcaaaaaga taagagggca
    46621 aggttctcaa gtcgtcaaag aagaaaccgt caatttccct gtatccaacc taaccagcta
    46681 ccaagaacta gttaaagaac ttggactgcg ctctaaaacc aacgtggtca gtctggacaa
    46741 gattattatt gataaaaaat cctcactgat aaccggtttc ccagagtttc ggatggtttg
    46801 gaaggtggtc cgccagcgtg tggtggatga tctggtatcc gttctggata cggactatct
    46861 ggatatggaa ctaatcccaa atctcactcg ccaaattgct gagcagtcta tctattctta
    46921 tatagaaaat ggcctcaaac tccttattga ttatgctcag aaggaaatca ccattgacca
    46981 ctcaagcgac cgagacaaga ttctcatgga cattggcaaa gacccttatg tcgtttcgat
    47041 taaatcaaaa gtctatctcc aagacggacg ccaatttcag tttaccgaaa gtcgccataa
    47101 gttagaaaaa tttagatttg tagattttgc aaaacgcaag aaataaaaga ctgagacacc
    47161 agatctcagc ctttttcttt atcccaaaac taattcatca ctgaccagac tttggccact
    47221 atttttctgg aagagatgca taaggtcttc aactgtcaga tgctttttct cttctccctt
    47281 gacatccacc actatcttgc cttgatagag cataatgaga cgattgccgt actcaatcgc
    47341 atgattcata tcatgggtaa tcatcaaagt cgtccactga tggtgctcca caatcttttg
    47401 cgtcaagtcc atcaccattt gactggtttt cgggtcaagt gccgcagtat gttcatccaa
    47461 aagtaaaagt tttggtttca tcagatctgt catgactaga gtcaaagcct gtctttgtcc
    47521 tcctgagaga tattgagtat ctacttttaa gcggttttca agaccaatat tcaactcctt
    47581 caaggcctct tggaactgga ttctatcctt ctccttcacg ccccaaccaa gccctctctt
    47641 ctgcccgcgt ctcaaggcaa tagccatatt ctcctcaatc gtcaaacgag aagctgttcc
    47701 catcttagga tcttgaaaaa cacgggcaat atccttggct ctctttctga cactcagatt
    47761 tttaatggat ttccctgcca gtagaaggtc cccttcgtcc accgatagat tgccagccaa
    47821 gatattcatc aaagtggatt ttcctgctcc atttccacca atcacagaga taaaatctcc
    47881 ctcttcaacc tctaaatcta atcctttgag gacatggttc tcattgaccg tccctgcttc
    47941 aaatgtcttg tgaatatttt caagtgttaa caaacttgcc ataactttct cctcctattc
    48001 atttctcaat ttcagaccac gaatctttaa tctcttttgc aattctggtg caaataaaac
    48061 caaagcgagc aagattgcat taaagagacg taccaagttt tgatctagat ttggaatttc
    48121 atagatattt aaaataatca aacggtaaac aatagcaccg attccgatgg ataataagcg
    48181 gcctccaatg gtcaagtcgt gtatcaagac ttccacaata atcaccgagc tcaaaccaac
    48241 aacaatggtt cctgtcccag aagtcacatc tgaaaatcca tcattttggg caaacaagga
    48301 accacataga gcaatcaaac cgtttgaaat catgtaacca acaatcttca tggtgtctac
    48361 attgactcca ttggcctcac tcatcgggat attatcccca gtcgaacgca agaccaagcc
    48421 aatctctgtt ttcatcaaaa gggtcaagac caaacaaaca agtaaaaaac aggccaaact
    48481 aagtgagaaa acagttcctt catttgtcag acccaagctc gccaactgtt taaagacggt
    48541 tgcagaatct cccaaggaaa gattgggtac actgcccatg attttaatat tgattgaata
    48601 gagccctgtc aaggtcacaa tccctgtcaa gagagctggg attttcatct tggtgtggag
    48661 cattcctgat acaagacctg ctaccatacc tgccagcaaa gcaagtaaag tcgcaagcca
    48721 aggatttgcc cctgcctgta tctgtgatac aacgactgct gcccccagag gaaaggctcc
    48781 ctcagcagtc atatccgcaa tatccaaaat acggaaagtc aagtagaccc caatcgccac
    48841 aatcgaccac aacaaacctt ctgataaact agataacaca aaattcatct taatcctcct
    48901 tattccttgc cttctaactt actaatatca attcccaatg catctgccat ttcttgatta
    48961 gtatgcaatt ccagtttttc aggcaactca actgctatat tttctggttt ctcacctttt
    49021 aaaacatgaa tcagcatgcg agctgtctgt cttcccaatt cttcataatt ggttccgtag
    49081 ttatacaagc caccaacagc aatcatttct gttgaaccac cgaatactgg aaccttgtgt
    49141 ttaatagaaa cctgcttaac tgtttccatg gttaacgaaa tgatattatc agttgggaca
    49201 aaaaccatat ccacctctgc catcaaactc tctgctgccg ccttgacgtt attactgtcc
    49261 aagattgttt tttcaacaac ggtaaagcct ttttctttca gaagtcgctt agcttcatcc
    49321 ttttgaacaa ccgaattggg ctcgctctga gcagaaggcc ttgcgatggc tagaacgaca
    49381 tcgctggatt ctgctagttg ctgggaaatc gtttttagat ttctttgttc tccctgtgca
    49441 ttttgcaaat caatctcaat attttccccc tcaacatagc cttgtttggc cagctcatcc
    49501 acaaatcctt ctctagtagc gtctaaggac tggtgggtaa taaactgcga aataccgata
    49561 cgaaagacat ctcttttctt atctgccttc aactgatgca aactgatcag agaagtcaag
    49621 aggatcccca ctaccaagag ggggctagtg attttcgaac aactttcata atgaaactcc
    49681 ttctcagaaa agataaaaca aaaaaccgcc tagatactat ctaaacggat acttgaaata
    49741 acctaacaag ttacaactta gctagtccat ttagatattc atctatatgg accacaatca
    49801 aaaatcttag ccacatagat acaaacaaat tgcttgaact gtttgcatcc atggtgacat
    49861 gtctacaaac actatctaaa gtagctaaag taaaagtttt gattcatagt tacggcttgt
    49921 ctcttattca tttctttttc tgctttcttt tttgatgctt cctgtcctac cacctttttc
    49981 atcacctgtc aataacattt cataattttt aaaatttttc aaaaattctt tcaggaagct
    50041 ttcaaagttt ttcaaatcag tccaacaaaa aaagttttat aatttccata aaaattgaca
    50101 tggaaattat aaaaccatta ctagtttagt cctttttgat aacgtgccaa ttcggcttgg
    50161 ttcgcccaaa catagtgacc tggacggatt tctaccatag atggcttatc agtctcatag
    50221 tcgtgttgac ttgggtcgta aaccttcaag accttcttac gttccaagat tggatctggg
    50281 attggtaccg ctgaaagcaa ggcttgagta tatgggtgaa ttggattgtt aaacaattct
    50341 tctgtttctg caacctctac aataacaccc ttgtaaataa ctgcgatacg atctgaaata
    50401 aagcgaacaa ccgacaagtc atgggcgatg aagagatagg tcaggccgag ctctttttgg
    50461 aattttttga gcaagttcaa gacttgggca cgtacagaaa cgtccaaggc tgaaattggc
    50521 tcatctgcaa taacaaagtc tggttgcatg accaaggcac gggcaatacc gatacgttga
    50581 cgttgaccgc ctgagaattc atgagggtaa cgagtcaagt gctcagcaag aagacctact
    50641 tcacggataa tattttgaac tttctcttta cgttcttctt catccttaaa taaacggtga
    50701 ttgtaaagac cttcagaaat aatataatca acagtcgcac gttcattcaa acttgcggca
    50761 gggtcttgga aaatcatctg gattcgacga atcaattccg cagcttgttc acgcgatttc
    50821 ttaccattaa tcttttgacc atcaaaaatg atatctccat tacttgtatc atttagaccg
    50881 atgatagcac gaccaatagt tgttttccca ctaccggact cacctacaag cgagaaagtt
    50941 tctcccttgt tgataaagaa gttagcattt ttaaccgcga caaacttctt acttccttca
    51001 ccgaaggaaa tttctaaatc tttgatttct actaattttt cagacatttc cttcctccta
    51061 gtcagccaga tgggcaaatc ccattttttc acggatctta tcatggagat ttgcaatcac
    51121 agctggtttt tctacttttg gagcatcctc atgaagaagc caagttttag cccaatgtgt
    51181 ctctgatact gagaattgag gagctttttg ttcgaagtca atctgcattg cgtagtcaga
    51241 acgcaaggca aaagcatccc ctttcaggtc agtataaagt gacggaggtg ttcctgggat
    51301 tgagtaaagt tcccctttat catcagcaag ctgaggcaag ctagacaaga gactccatgt
    51361 atatggatgg cgagggtcat agaagacttc ctcaaccgtt ccatactcaa cgatttctcc
    51421 tgcatacata accgctacct tatccgcaat acttgccacc acaccaaggt cgtgggtaat
    51481 aaagattgtt gtgaaatgat actcgttttg taaagatttt agcaaatcaa taatctgagc
    51541 ttgaatagtc acatccaagg cagttgttgg ctcatcacag atcaagacat caggtcggca
    51601 ggcaagggca atcgcaataa cgatacgttg acgcattcct ccagaatatt ggaatgggta
    51661 ttcattaaaa cgtctatctg cgtctggaat gccaacctta ttcatgtagt caatggccaa
    51721 ttctttcgct tctttagctg tttttccttg gtgttttaca ataacttctg taatctgact
    51781 accaattgtt ttaatggggt ccaaactagt cattgggtcc tggaagatag tcgcaatctt
    51841 agcaccacga atttgttccc aatccttgtg agaagataaa gctgtcaagt cctgaccacg
    51901 gtagtcaata ctaccttggg caatacgacc attttcttcg agcatacctg tgaaggtctt
    51961 tgtcaaaaca gatttacctg atcctgactc acctaccaag gctaatactt ctccttcgac
    52021 tagttcaagg gaaacgccgc gaatggctgt caatactttg tcacgaacgt caaattccac
    52081 gacaatatcg cgagcagtca aaattacatt tttttctttt gtcatttcta ctcctatcta
    52141 tgtgtacgtg gatcactagc atccgctaag ttttgaccaa ctacgaaaag ggacaaggat
    52201 accaagacaa gggttgtcaa tggaatccag aacaagtaag cattggttgt tacgttttgt
    52261 gaataatccg aaatcaaacg acccaaactt ggcactgtaa tcggtaatcc aagaccgaag
    52321 aaagacaaga aggcttcgta tgagataaag cttggaagca tttgagtcat ggttgtcaca
    52381 ataacagata ccaattgagg catgatattt ttggcaacaa tcttcaaggt tggtgttccc
    52441 aaagtacgtg acgccaagtt gtattccaag tcacgatagc gcaagatttg cacacggatc
    52501 atgaaggcaa taccaatcca tgttgttacg ctcatggcaa aaatcagatt ccagaatcca
    52561 gctccgattg agtaagttaa gacaataaca atcaaaagag gtgggatgtt tgagatgacg
    52621 ttgtaaactt ccatcatgac acggtcaact gattttgaaa taccccaaat accaccgaca
    52681 aaaacaccga taaccaagtt aatcactgtc gcaatcacag aaatgaggat ggagttacga
    52741 gctccgaacc agacaccgtc aaagagcgat ttaccgttac tgtcagtacc gaaccaatgc
    52801 tccgcatttg gcttgatata acgaacacta aagtcgttta ccttgctgac atcattgaaa
    52861 tcaaacttag aaaacattgg gtagatgaaa cttatcaaaa tgatggctac caagattccc
    52921 aacatgacta cagttgattt tttcttcata aattgtttaa acactgattt ccagtaagaa
    52981 tatgctggcg catcaatagt ttcagaggca aaatcgtcac gttttacaaa ctgaaatttt
    53041 tctttatcga ttgtagacat tatttgcctc ctttctcagt caatttaata cgtgggtcaa
    53101 taatagtcat ccaaatatct cccaaaagac gtgagaagat agaaatacat gtaaagatga
    53161 agacaagacc aacgaccata gagttattag atgcttttac agagtcaatc aacattttac
    53221 ccatacctgg gaaggcgaag actgtttcag taagggttgc accaccgata accccaataa
    53281 tggcaccagg aattcctgaa accagcggaa ccatggcatt tttaaagatg tgtttgtttg
    53341 aaatttcttt ttcagacaaa ccttttgcac gagcgaaacg aacaaagtct tgagattgca
    53401 agtcaatcat gtaacgacga atccaaatgg ctgtaccagg agcacccaac aaaccaagga
    53461 tgactgctgg taaaacgtaa gaacgccaat ctccagctcc caagataggg aatgaatctg
    53521 gaagggaaat agatgatcca atcaatcgaa cgatgtaaac caaggcaatc gttggaagag
    53581 caagcaagaa ggtcaaagcc cctgttgaga ggctatcaat ccaagtgttc ttgaaacgag
    53641 ccatggctga accaagtggc acggcaagag cataggcaag aaccaaacca atcaaaccag
    53701 taatagcaga gctgacaatc atagatggat attggtaatt actttcagtc gctgtataag
    53761 gatcatcttt cccatagcta gctacttcac gagagtcagc ctgactaggt gacttgtagg
    53821 ttcttgagta aatatttaca gaagacgttt tcttacctgt tgggaactga acttgggcag
    53881 ttttggtttg tccttgacct tgagtaataa cctgaagaac tggtgtatta gcataggttg
    53941 ggtaagagtc acctaaattc aagttcacaa agttttgatg aacaaatggg aactgactgt
    54001 taaagtacaa gagatattta tgtttagttc ctgaaccgac caatgaccat ccgatagctg
    54061 gatcattttc aaaacgaagg tagcgtttca agtctggatt ttcagggtct tggattttat
    54121 ttgtatggtc aatgtcaatc aagttagcat agaagtgaaa aacacgttca aaaattggaa
    54181 tttcacgagt agcatagaat tgaccacttt cagtaaattc tcccaaagtc caaccatgac
    54241 ctaattgatt gatgtacttt tcataaatag ctttattggt cgcatttgct tctactgtta
    54301 cagaagaatc catgctactt gccttttctt gcaactcttt agtatcgtaa tactcaatgt
    54361 agcccatacg ctcaaacaca gtattttcat agttatcacg tttatcagcc gttgtcgcaa
    54421 ttttattata gttaggatcc tgcttgaaaa tcaattttcg aggaaccaag gtatagataa
    54481 tcgtgtaggt caaagtcgtt actaagaaaa tcgaaaccaa tgaccgcaaa acacgcataa
    54541 aaatatattt tttcatatta tttcctttaa aaatcccaaa agaaccttct cctcatggag
    54601 agaaagttct attagaaatt atttacttca catgacttgc caattctttt tgagctttct
    54661 catttgattc agctttttct ttcaaccatt tttcacgagc tttttcatac tcttccttag
    54721 tcaccgcttt atcttgtaat ttcaaatatt tgaagtaaac atctgacccc ttagagcctg
    54781 tttgcgcaga agctccagta aatggaacaa ttcgtgaaag cactggtgct gcaccagaag
    54841 aagccatagc aggaataaag agtgaactat ctgtcaacca tgcttgagcc gctgcatatt
    54901 tttcataacg gacattcaag tcgcttgtct ctttggcagc ttcatcaact aatttatcgt
    54961 attctttcaa accaacttga actactgaag ggctatttgg attatcaaat cctaaatatg
    55021 tttttgtagt ttcactgcta gttgttttta aaatatccag gtaagtagat gggtcttgat
    55081 agtctggccc ccatgaaact cctcccgata catcccaatc ctcagatgaa gcattggcag
    55141 catagtaagt aatattaagg aattcatcac ttgtcatttg ttgaatatca acaacgacat
    55201 tttcaacacc aagaactgtt tctacagatt gtttaaagga ctgaatacga gatatatagt
    55261 tttttgatgc ttggtctact ggaacgtcca gatgaatagg aaactgaacg ccgtctgctt
    55321 ctaaagcttt cttagctttc gcaaactctg ccttggcctt gtcagcattg aataaaccat
    55381 cctgcccatc agctaaattc acacctttcc actcatcacc ataagcagga agttgagcag
    55441 cgactaaatc accaaaggtc ttctcaccag ctgaaacaaa gtctggtttt acaaataaat
    55501 tacgaactgc taaagctgct ccatctttac cattgatttg agctgagtaa gctgagcgat
    55561 caagagcaaa attcaaggct tgacggaaat ctttgttaag caatgccttc ttagtagcta
    55621 ctttctctga atctgtagtt ttagaagtat agttgtaact ttggcgatca atattcacac
    55681 ccagaccagc aatcccagag cctgattgtg tgtaatagat attgtccttg tattcttctg
    55741 caaccttaga atagttggag ctggtagggt aaagacgggc ataactatag gctccactag
    55801 tgaagttacg ctctagcgac tcctgatctg atccatcata gtaagctaga ttgatagtat
    55861 ctaggtggac attttcttta tcccaatatt gctcattttt tacaaactct acagaagatt
    55921 ttgcagtcaa ccctttcaac aagaatggac cattataaag caaggatgtc ggatctgttg
    55981 gtttagcaaa atcgcttcct tttgatgttt cgaattcttc attcagaggc cagaaaatag
    56041 aataggtcaa cttagagttc cagaacggtt caggctggtt caaagtgtat tgtaacgtat
    56101 aatcatcaac cgccttgaca ccaactgttg aaaaatctgt tgaagttcct gatagataat
    56161 ctgccaagcc tttaaccgaa ttttcagcta aatacatagc ttctgatttt ttatctgctg
    56221 cgtgttttaa accgttcacg aaatctttag ccgtcacctc tgcatattct tctccatcag
    56281 aggtaaacca tttaacccct ttacgaatct tataagtgta ggtcaaacca tccttagaga
    56341 cttcccaatc ctctgcaact gcaggagcaa gattaccgta attatcgtta gtgaataaac
    56401 catcaatccc atttgaagtc actactgttg tactattttt acttgaaatc aggtagtcca
    56461 aggtttctgg gtctgctgta taaacatagc cataagcttt aggggctgat gaatcagatg
    56521 attttgaaga actgcatgct gcaagtacac ctgctgctaa taaaacaaga cctgctgtag
    56581 caaatacacg attttttttc attttctact cctctgttta tgtgaattat agattgacaa
    56641 ccattatatc acattatcca ttaaaaatca aacaaatttt cagaatattt aggcttgttg
    56701 gcacaaattt ttcatttttt ttgaatatat gattcaaatt gtcgttcgaa gtgtcaaaga
    56761 ctacagtgaa aataggaaat ttgacgcaga aactttggag cttaggaaga catacagtaa
    56821 aatgaaatac ggacggaaca atgtgatttt ggaattcaaa ttaaattata acaatattgt
    56881 agaagtatca ttctagtatt caagattcag tttactatgt cttttcacac caaccttatt
    56941 ccgaattcaa ttactttcgt gatttacaca tatagattaa gactatcttt tacccaaatc
    57001 attcatacct ctctcaacta gatgtaactt acaaaacccc tgacctcatg agccactttc
    57061 ttcctcctca tgaggtcagt tttactttct gctgttccag tatcgttttt cctcgctaga
    57121 tttcctcaaa agggcagact cctcccttgg tgcgtcacac gattttttca tctcgactgt
    57181 tctttaatgc atcattaacg acgcttttct tccaggtggt tcataaggaa caggaagatt
    57241 caggttgact tttctaatcc tagaataaag tgctgaaaac aattcggaat aggcatagag
    57301 actagacaat ttgaggagct gcttgcgtcc tgttcgaaca cagtttccta ccacgtgaag
    57361 aaaaagatgg tggaagcgtt tgattgttaa agtttggaag tcacctccag ctagatgttt
    57421 gagaaaaaga tagagattgt aggcgataca gctcatcatc atacgaactt cgtttttgat
    57481 taaggttgaa ctatccgttt tatcgccaaa aaatccctcc ttcatctcct tgatgaaatt
    57541 ctcggcttga ccacgtccac gataaagctg aaactggtct tggcttgttc cactcgtcat
    57601 atttgtaacg agagaaataa tatcgtagaa caagtttcct tcttttcgtt cagagaactg
    57661 gcagacacga cgcttgtgcg accaagatcc tgcttgatag agagtttctg agtaggcgga
    57721 gtggggcaag atggttaagt cctcatcctg tgggcaaggg agggaaaggt ctccaagacg
    57781 gctcagaaca gtatttttct tgagttttat gaggtagtat tgccctgttt tttcaattaa
    57841 atcgtataat tttggggtcg caaagccact atccattcga aagagaagtt gattaaaccg
    57901 ttctaaaaca ggtgtgataa agctgtctgc ctcttcagaa caataacgat taccaggacg
    57961 aagctgggca ttgaaacaat aacctgtctt cccctcgaaa gcataaagag gatgatagcc
    58021 atgagcacga tagtgggcgt tataagcaac accttcttgc ttgccataag ttgtgaaatg
    58081 ggtagaatcg atatctacaa tgagttggtt tagctggtga aactgtaaaa agaattcgac
    58141 caattcaagg ttgaggcatc gcaaactatg gactgtttcc tcgtcagttc tggaaagaaa
    58201 acgggataag gttggctgtg aagcaagctg ccctccttcc aacaattttg gaaagtaggc
    58261 atcagctgac aattctttac aagcatagtc cgttccatag cctgttaaca gttgaaagag
    58321 gaactggaca aggatatctg aatccgaata acgacagtag cggcgttggt cattcgttac
    58381 taaatactta gaaatcagct cttttagttt caactgggaa aaaagttcct gaaaaaagat
    58441 aagaccacca tactgggtta aatgacctcc atcgaaagat agttggtaaa aagacttgtt
    58501 ttggaagtga tgatttggta aactgttcat gtgagtttcc tttctttttg tgtttttttc
    58561 tacacttata ccataaaggg gaaactcttt tttgtctagt aaaaaacacc cattgggtga
    58621 aaaaagaaac catccaggat ctaagctaag gcaaggattc tggatggttt ttaaattagg
    58681 ggtgaataat tggggtttta tactttaaaa tttctcgcta ccttatccac tatatgctcc
    58741 tcgctatcac gtttctattc atagcctacg atttcactat tgctttctct gacaattctt
    58801 atttcctgcg tcagacttaa aacgatctat ccccagacca ttttaatccg ctacctcacg
    58861 atagtcaggc ttggggagcg ctattgtatt caccggtagt ggatccctac agaggactta
    58921 cacctcagat gcacgacatg cccatcgtat aaaaaatctc ctacccaagg tagaagattt
    58981 caaacttata aaacttaatc cgtcatgtcc gataccaaca ttcgatgctc caatggaata
    59041 ctgcacataa ctagcaagaa aataaagcct gactgaatcc agaagagagc caagtcaaaa
    59101 attccgtgca cagcaaccac tgtaaggaaa gatagataaa ggccgataat cggacgtttc
    59161 cccgactcct gactcatatc catcatcaag cgaacaggag caacagaaga cagaactaat
    59221 aaaatggtac ccacaattcc gtaactcaga atcgtatcaa tataaagact gtgggcatgt
    59281 tcatgataag gagcatgtat ccgaggataa gagtgcatat aggtcaatgg cccttcaccc
    59341 caaaaaggat tttgcttaaa caaggccatc ccagcatccc agatagaaat gcgttcttcc
    59401 atagaagagt ctaaagtacc cattcgaact cccaaatcac tagaaaagag gaaactcaaa
    59461 ccaatcgcga agaccccaat actaagccaa aaggccttcc agtttttaat agtcgtaaag
    59521 agatagataa ttgctccagc gataatagca ggaaaggcag ttcgattttg agtaaagttc
    59581 aaaccaaaga gattaacaaa gcctgcaatc acacagaata ctttcaacca attcaacttg
    59641 gtcgttgtaa acagatagaa agcaatcata atacagaaac aacaaataat tccataataa
    59701 ttaggattaa agaaggtcac ttctgcccgg ttctgatgcc acacctgcat attgggggaa
    59761 agaaaagcat aattaaattt cttcacaatt tggaaatgtt ctaaactggc aaaagcagct
    59821 gacaagacac taccaaacaa gacgaactgc aaaatcaatc gaaagaattt atgggataaa
    59881 atcgactgat agtgcaaaaa gaaaatagta aatagaaaca ttcctactga agccacaaga
    59941 cccatccaat tttgtgcaag aatggatata acagtactat agccaagaaa aagaagcagc
    60001 atcgaatgct cccccatttt ctgaagaata cttttcatgt ctcctgtaaa aatcagactg
    60061 ataatatata aacagaatac aactacaaaa agataaaagg gtaaaaagat actcaggata
    60121 attcccaata aaatcagctc tttactagac aaccccttca gcttttcaat aaagcctatt
    60181 gatttcaaaa tgaatccttt ctctccaaat cagctgattc agataatagt aagctatcct
    60241 atattgtacc acttttttag caatttgaaa acaaaggaaa cgttttccaa aataaaaacc
    60301 ctattttatc caccatatca aggcttcaaa atgttacttc aactccattc tcaattaccc
    60361 gataagtctg attttgcaag tcaatttctg ctactgctgt tacggactta tctttatttt
    60421 gacgtttgat tacaatgctg tgagctgttg gtgtctctat ctcagtagtc ccttctagat
    60481 caaaggcttc tgaacggtta cggaaagaaa atagattgag aagggccttc acaacaggtc
    60541 gttgcacttc ttttgctatt tcctcgttgc tatagtaatg acgattaata tttcgacctt
    60601 ctttagtttc ttctaataat ttcaagtcat tcttgcctgc taatagaccc acatagtaaa
    60661 cctgaggaat acctggggca aaagcttgaa ttagacgagc gagaaaatac ttgacatcat
    60721 catctccaag cgctgaatag taggttgaat tgatttggta gatatctaag ttgttatact
    60781 cggcactaga gtacttacgt ttgacattgg ctccaacctt atagagttca tttgaagcat
    60841 agtcaatctc ctcatcggtc aggatatcct tgacatctac tactccaatc ccatcatggg
    60901 tatctagcgt cgtaaattgc ttcatcgggc tcatctttaa ccacttagcc aaacgctctg
    60961 ttctggaact gtaaagagta taaagtgtca ccattggaag agcaaaatca taaacatagt
    61021 aatcatggtc tgctatttta aactgaatcg aatagtgttc atgaatctca ggtaaaagct
    61081 ctgtcccata ctcagcagcg atatctcgaa ctttgtccaa taaatcccaa atatctggtt
    61141 ccacaaagaa atcattagta tccaatttct tcactgcata agcaaaggca tctagacgaa
    61201 tcaaatcaca cccattactt gccaagtgct gaatggtctt acggataaat tccatagtta
    61261 cttctttggt cacatcaaga tcaatctgct cctcaccaaa ggtattccac aaatgttcca
    61321 ctgaaccatc ttcaaacaca atctcttgct ttggtgcacg atccttacgc ttgtaaatta
    61381 aatctacatc agactgtgtc ggacggtttt ctggccaaaa cttatcccag tttaaaaaga
    61441 gagctttaaa ttcactggct tcatgttttt cttgatagtc cttataatac ttggattgac
    61501 gagaaatatg attaatcata aaatcaaaca taagataata tttctcacct aaacgcttaa
    61561 catcctccca atcaccaaaa gctgagtcca cttcgtcgta gtcaactggc gcaaatccac
    61621 gatcacctgt tgatgggaaa aatggtaaaa ggtgaactcc tccaatagca tctccaaaat
    61681 gctcttccaa attatcatat aagtctttaa gattatttcc aaggctatca gaataggtaa
    61741 tcaacatggt tttattttga atcggcatca ttactctcct ttttctaatt gaagccaagt
    61801 ctcatatgat ctggcttcgt aaatcaaatt cattttaaat ctctatttat catcaaactc
    61861 gtactaatat agactgtgat aaacaaagta ctactttctt gttttctaca tagaattatc
    61921 aacaagctga actcttcctc tgtgtcaaag actatagatt ccatgagctc ttcttatact
    61981 cttcgaaaat ctcttcaaac cacgtcagct tcaccttacc gtaggtatgg ttactgactt
    62041 cgtcagtttc atccacaacc tcaaaacagt gttttgagca acctgcggct agcttcctag
    62101 tttgctcttt gattttcatt gagtattact tcactgcccc gttgctcatt cctgaaatga
    62161 tatggcgttg gaagaagaga tagacaatgg tgatactgat aatgccgacc acgtaagagg
    62221 caaagcttgg tccgtagtcg ttgaaatatt ggcctgcgta gttgtattgg aacaaaggca
    62281 gagtccacat tttggaatcc cggttcaaga caaggagtgg caacatgaag tcattccaga
    62341 accaaagggc attgatgatc atggttgtcg catgcatcgg tttcatcatt gggaagatga
    62401 tgcggaaata ggttgtaaat tgattagccc catcgatctc tgctgcttca tccagacttt
    62461 ctggaatcga gattttgata tagccaacat agagaaagag ggtctgtgga atcgcatagg
    62521 tcaagtagag caagatcaaa ccaaaggtat tagccaaacc gagtttactc atcataaccg
    62581 taatcggaat catgatgact tggaaaggta cgaagattcc gaggattaag agggtataca
    62641 tgatggtaaa gacttttctt ttactcatat tgcgagcgat ggagtaggct gccataggga
    62701 taaagatcat tactgcaagt aaagacaaga cagtgatgac gacagagttc caataatagc
    62761 ctccaatccc atcagctaag agacggctaa agttgtccca tgtgaagttg gttggaaagc
    62821 caaagaaatt atctacaata tccttagtgg gtttgaagga actaaagagg gtagcaagga
    62881 gcggcactaa aatcagaacc gatcctagaa tcaatagaat gtatttgcca atcagggctt
    62941 ttctttcatc ttgtttcatc atgcttctcc tcttaaattt caaatttctt agatactctc
    63001 aattggatga tcgaaatcac tacaattaag aggaacaaga ttacggcaat ggcattggca
    63061 taaccgaatt ggttgttttt aaaggcatag ttataaacca agagcccaag tgaggttgtg
    63121 gcattgtttg gaccaccacc ggtcatggca aagacttggt caaaggcagt cagcccacct
    63181 tttagggcta ggataaagac catagagaca cttggtagca agtaaggcaa ttcaatgttc
    63241 cagaaaactt gcttgctagt cgcaccatca atccttgctg cctctgtaat ctcagttgga
    63301 atagattgca aaccagctag gaagatgatg atgggcatag ccaccccttg ccaaagaagg
    63361 acaaagacag ccgcaaagat tgctccccac ttagtcccta aaagactggt ttggagaaat
    63421 tcaatatgaa gggcattccc aatcgctgga agaccgtagt tgaagacttg cttgaagatc
    63481 aaagccactg tcaaaccaga taaaacagct gggaagaaga accaagcacg gaagaaggtt
    63541 tggcctttga ttttagagtt caagacacgc gcaatgaaga tcccgagtgc aatctcacca
    63601 accaccatgg caatcgtaat gattgcggta aagccaatcg cattcatgaa ttttggatcc
    63661 atgaagagga gcttaaagtt gtttaagccg acaaatttgt agttgtaagt caatcctgtc
    63721 cagttggtaa aactgtaaaa ggctccttga aacatcggca catagaagaa aattgcttgt
    63781 aacaagaggg ggatgaccac aaaagcccat gcccaatatt tttgtaatac ttttttcata
    63841 gtctctctac tcctaatcca catccgcttt catcgggtta aagaaggcat tcaaatcatt
    63901 gaccatgcct tgtttatcac cggtcaagac atagttcatg gtcaaggtat ggaagtctgc
    63961 ttcactggtc cagtattgtt gcaaccagac caagtgacga tccgtaaagg catattcggt
    64021 cataccagca agcggtgaat cttctcctgc ttgtttgacc ccttcgatcg ctgttggaga
    64081 tccgtccaca tcgtagtatt tttgcatgac ttctggacgg gtcatatatt ccacaaaggc
    64141 attggcttct tttggatgtt tggtggtggc tgagatagac catgccaagt ctcccgcacc
    64201 aacggttaag ctttgtcctt tttcttttcc tggaatcatg aaggttccaa tcttaaagtt
    64261 cggtttttgt tcattaatcg ctgtgatcgc ccaagaccca tttggtgtca tgaggacatc
    64321 cccacgtgcg aaggctccga taacatcggt atagccagca ccttcccagt tcttttgctt
    64381 agagccattg atgcgaagga tgtccatgac cttgatatca tctttcataa tcggatccga
    64441 caatttaatg gcatttggtt gagaataacg aaggtattga tttgcttctt ttcctccacc
    64501 tgttgctgtc gcaaaggcta attgattgta accattgagt gtccaagcat ctgcacctgc
    64561 aattccaaat gatgtttgtc ctttagcaac gatatctttg actaactgtt caaattcatc
    64621 ccaggtttca ggaaccttca agcccagttc ttcgaattta tctttgttgt agtaaattcc
    64681 ataagcatta gctgtaaaag gaacgttata aactttttcg tttacagcat atttttcagc
    64741 gtaggcattt ttcacgcgtt tcaggtagtc tttgttgctc aaatcttcaa aaacacctgc
    64801 ttttgcccat tcttgcagtt cgatggactg tgggtaaata ttgaccacat caggcacatc
    64861 tcctgcgaga acgcgtgtct tcaatacttc accagcattt ggtacattga cgactttgac
    64921 cttgatctta gggttttcct tctcaaaatc acgagtgatt tcttccaagg ttttggtcat
    64981 ttcttttttc tggttgaaat actcgatggt cactgtgcca tccgcagatt taccatagtt
    65041 ggagcaagcg ccgagcccaa acaaagctaa acctgtagtt gcaagaagtc cgattttttt
    65101 ataccattcc attagaaagc ctcctttata aatttataca cccttattga actgcacccc
    65161 aaaagttaga cagaataaat ctaacttttg gggtcagtac atatcatagt tttctaaaaa
    65221 tatactgtct actcaaaaaa tctccttggg ataagataac agttaagccc gcatacatta
    65281 gttctgcacc tgagtaaact tcgccatttt cctgtaattt atatagtccc tcttcatcca
    65341 aatcttttaa ttttaaagtt gtttccatgg tctctacaac agataaaacg cgaacgtagg
    65401 ttacaatcgt ttgatttccg taattaaatt gtacagctgc ttcattggat gcagtatcag
    65461 gattaattag tctatactgc tgtcctaact gaactactgg tcgtaattct ttatacaagt
    65521 tcacctgatt agcaatcgta gctttctctt catctgataa atttgtcaaa tcaagctcat
    65581 agcccaaatt tcccatcatt gctacatggc cacgtgtttc taatggtgtc attcgtccca
    65641 tctgatgatt cggtactgct gacacatgag cccccataga aatggttgga tagagatagg
    65701 atgaaccgta ttgaattggt aaacgtgcaa tggcatcagt attatcacta gcccagactt
    65761 gtgggaaata gcgtatcata ccaagatcat ttcgtccacc accaccagag caggactcaa
    65821 agagaatatg gctgtgcttc tctgtcagat aagaaacgag ttcgtaaagc cccagcatgt
    65881 actgatgaga ttgcatctgt gtctctagat aagttaatcc attccctagc ttagtgatat
    65941 tgcggttcat atcccattta atgtaatcaa tctcatgata agataggagt tgatctaaga
    66001 cacttttcaa gtattctact acctgaggat tggcaagatt aagtactaat tgattccgag
    66061 aataagtatg ctcataatca ggaacctgaa tagcccagtc aggatgttga cgatacaaat
    66121 cactatctac agaaatcatt tcgggttcta accaaagtcc aaactgcaaa cctctttcat
    66181 ggatagctga aatcagactt tctagacttc cacccagttt ttcctcatta acaacccaat
    66241 cacctaaagc acgattatca tcaaaacgat tgccaaacca accatcatct aatacaaaaa
    66301 gttcaatgcc aactttctta gcttcatctg ctaactctaa cagtttttct ctctgaaagt
    66361 caaagtaagt agcttcccag ttattgatta gaattggacg ttctttttta gaaaattcac
    66421 ttagcataat gtgcttcagt acaaaattct gactttcatg actaatacca gttaatccct
    66481 gatctgaatg agtcactaaa gctaccggtg tttcaaagta ttcctcaggg gctaacttcc
    66541 aagaaaagtt ttctggatta atgccaatag ccacccgaac ttcattcaat tgattttttt
    66601 gaacaaaagc ttcaaagttg ccactataca ttagttgaat agcaaacaca ttcccagcat
    66661 cctctgtgac tccttgttcg catagtagaa gagctggtgt ttgagcatga ccagaagcac
    66721 ctcggtttga actaatcgaa aagattcctt gttctacctg ttgacgtcta acagtctttt
    66781 cacgagcata agcaccctgc agagttgcta tttcataatc tgcagctgga aaatcagcca
    66841 taaaagaaaa atctttatgg atgacaactt cctgattact attattatct aatttactgt
    66901 agctagtaat agtcgcatca ttattaaaag tagtataata caaagtcaga ctaagttgag
    66961 ccttagaatc ttctaacatt aagacaagag tctctgtatc gtccatgcta tgtggagaag
    67021 gtaagccctg tggaccattc tgacctttta aaatctttgc ttctacaaat cgaaagtctg
    67081 ttacttcagt tacactatgc tgaacctgta tagttggttt cctaaaatct cctaagccat
    67141 gttgtccaaa aatctgtcgc tgagtatcta aactaaaggt tcgattagta gccgttggat
    67201 ttcctgaaaa ggcatggtct cgttcataaa cactattgga acctttatag ttcttaatag
    67261 tctttcctaa atgtttcaaa agtaagtagc catttcgatt ttcaataatc aaacttagat
    67321 ttttactctc aacataaaat agattattct ctatcctaac tcccatttac ttcacctcat
    67381 cactttattg attatatttt atcacctgaa atcgctttcc aaaatagaaa aatgtctcaa
    67441 gaatatggta aaatgttagg taggaggtag cacatgttag ttttttcaga ataccagact
    67501 ggaacaatcg accttgccct aagcttttat ggatatgagg aatgcacacc taattactct
    67561 tttggtccag ccattcgtga tacatacgtc ctacattaca ttactaaagg acaaggaaaa
    67621 tttcattaca aggataaaat tgttgattta aaagaaggag atttctttct attaaaacca
    67681 gaggaactaa ccttttatca agcagatagt aaagaacctt gggcctacta ctggttagga
    67741 atcactggag ggaaatcccc tgattatttt gctctttctc aaatttctga tcaatcctat
    67801 ctcatccaat ctgaaacttg tcatacccag actactgcaa aactcatctc agacattgtc
    67861 cgcttcgctc agattacaaa atcaagtgaa ttagctcaac tccatatcat gggacaactt
    67921 catgaactga tgtttcatct gggaactatt gctcccaatc agaaaaaaaa gaatatttca
    67981 tcaacccacc aactctatct tgaatgcaaa cgattaattg atagccacta tcctcaatca
    68041 cttacaattc aagatttagc aaaagaacta tccgttcaca gaagctactt atcaagcgta
    68101 ttcaaagaat ttaatacctt atcacccaaa gaatacctac tctacgttcg aatgcaccga
    68161 gctagacaac ttctcgaaaa tacccaagag tccatcaagg taattgcata ctcggtaggt
    68221 ttttcagatc cactccattt ttcgaaagct tataaacaat actttaatca gactccaagt
    68281 catacaagaa aagaatactc tcaataccaa ctagtgagaa aggcaacatt atgaaatcct
    68341 accaagctgt ctaccaaatc ctatctaaag aaaccgacta tatcagcgga gaaaaaatcg
    68401 cagaaaaact atccctaagc cgaacatcaa tttggaaagc tatcaagcga ctagaacaag
    68461 aaggcattga aattgatagt atcaaaaata gaggatataa actaatgaat ggtgacctta
    68521 ttcttccaga gattctagaa gaaaatcttc caattaaagt cagctttaaa cccgaaacaa
    68581 aatcaacaca actagatgca aaagaagcaa ttgatttagg ccatgaagca aataccctct
    68641 atctagcttc ctatcaaaca gcaggccgag gacgttttca acgttccttc tactcaccac
    68701 aaggtggtat ttatatgaca ctccatctta aaccaaatct cccctatgac aaattaccat
    68761 cctacacact acttgtagct ggagctgtct acaaagctat taagaaccta actttaatag
    68821 atgtcgacat aaaatgggtc aatgatatct atctaaacaa tcataaaatt ggaggaatcc
    68881 ttactgaagc aatgacctct gtagaaactg gcttagtcac agatatcatt attggagtag
    68941 gtatcaattt cactattaaa gacttccctc aggaattaaa agaaaaagct gccagcttat
    69001 ttaaagctac agctcctata acaaggaatg aattgatcat agaaatctgg cgtgctttct
    69061 tcgaaacacc agcagaagag ctattatacc tatacaaaaa acagtcattc attctaggaa
    69121 aagaagtcac tttcacacta cagcaaaaag actacaaggg acttgctaaa gacatctcag
    69181 aaaatggaaa acttttagtt caatgtgata acggaaaaga aatctggcta aatagtggcg
    69241 aaatttctct caatagttgg aagtaaaata acacaattat aatataaggc tctataatat
    69301 ttgtagtggg taaatccact atagatatta tggagcctat ttttgtgtag aaaaaaagtc
    69361 ccataagatt tataatgaaa agcgatcaaa caactcatta gaaagattca tatggaacaa
    69421 ttacatttta tcacaaaact actcgatatc aaagacacaa atatccaaat tatagatgtc
    69481 gtcaataggg attcacacaa agaaatcatc gccaaactgg actacgacgc accatcttgc
    69541 cctgagtgcg gaaaccaatt gaagaaatat gactttcaaa aaccttctaa aattccttat
    69601 cttgaaacga ctggtatgcc tactagaatt ctccttagaa aacgccgttt caagtgctat
    69661 cactgttcaa aaatgatggt cgctgaaact tctatcgtca agaagaatca ccaaatccct
    69721 cgtatcatca accaaaaaat tgcgcaaaag ttgattgaga agatttctat gaccgatatt
    69781 gctcatcagc tggccatttc aacttcaact gttattcgta agctcaatga ttttcacttt
    69841 aaacatgatt tttcttgtct tcctgagatt atgtcttggg atgagtatgc ttttacaaag
    69901 ggaaagatga gcttcattgc gcaagatttt aacaatctca atatcatcac tgttcttgag
    69961 ggtagaacac aagctatcat tcgaaatcac tttcttcact acgatagagc tgtccgatgt
    70021 cgcgtcaaaa tcattactat ggatatgttt agcccttact atgacttggc taaacagctt
    70081 cgctttcaaa tttctaggct caggctgaaa cagtctccca gactgtttca ctcccgaatg
    70141 ctaaaatcgt tcttgatcgc tttcacattg tacaacatct tagccgtgct atgagtcgtg
    70201 tgcgtgttca aatcatgaat cagtttcatc gaaaatccca tgaatacaag gctatcaagc
    70261 gctactggaa actcattcaa caggatagtc gtaaactcag cgataaacat ttttatcgcc
    70321 ctacttttcg tatgcattta accaataaag agattttaga caagcttttg agctattcac
    70381 aagacttgaa acaccactat cagctctatc aactcttgct gtttcacttt cagaataagg
    70441 aaccagagaa atttttcgga cttattgagg acaatcttaa gcaggttcat cctatttttc
    70501 agactgtctt taaaaccttc ctcaaagata aagaaaagat tgtcaacgcc cttcaactac
    70561 actattctaa tgccaaactg gaagcgacca ataatctcat caaacttatc aagcgcaatg
    70621 cctttggttt tcggaacttt gaaaacttca aaaaacggat tttcatcgct ctgaacatca
    70681 aaaaagaaag gacgaaattt gtcctttctc gagcttagct tttcttcaac ccactacagt
    70741 tgacaaagag ccttaagttt acggatctga agttttattg tctctaaaaa caaaaaagag
    70801 agttacaaac tctcattaaa acggagaata agggattcga acccttgcgc cagttacccg
    70861 acctaacgat ttagcaaacc gtcctcttca gcctcttgag taattctcca attaatgggc
    70921 acgagtggac tcgaaccacc gacctcacgc ttatcaggcg tgcgctctaa ccacctgagc
    70981 tacgcgccca agttaaaaaa cttggtaatt tgaacaaagt tcaaagcggg tgacgagaat
    71041 cgaactcgcg acaacagctt ggaaggctgt agttttacca ctaaactaca cccgcataaa
    71101 tactatcaat aaaatggcgc gagacggaat cgaaccgccg acacatggag cttcaatcca
    71161 ttgctctacc aactgagcta ccgagcctta ttgcgggagc aggatttgaa cctacgacct
    71221 tcgggttatg agcccgacga gctaccgagc tgctccatcc cgcgttaata taaaaggagg
    71281 atgtgggatt cgaacccacg cacgctttta cacgcctgac ggttttcaag accgttccct
    71341 tcagccggac ttgggtaatc ctccaatatt caaatggacc ttgtaggact tgaacctacg
    71401 accactcggt tatgagccga gagctctaac cagctgagct aaaggtccga caagatcatt
    71461 atagcggcga aggggatcga acccccgacc tcccgggtat gaaccggacg ctctagccag
    71521 ctgagctaca ccgccatgaa tcgggaagac aggattcgaa cctgcgacac cttggtccca
    71581 aaccaagtac tctaccaagc tgagctactt cccgagttaa atagaaaaat gcaccctaga
    71641 ggagtcgaac ctctaaccgc ctgattcgta gtcaggtact ctatccagtt gagctaaggg
    71701 tgctccatat tatgccgagg accggaatcg aaccggtacg atcgttacca atcgcaggat
    71761 tttaagtcct gtgcgtctgc cagttccgcc accccggcct ctctaagcga acgacgggat
    71821 tcgaacccgc gacccccacc ttggcaaggt ggtgttctac cactgaacta cgttcgcact
    71881 gttttcttct atctaaaaat gccggctaca tgacttgaac acgcgaccct ctgattacaa
    71941 atcagatgct ctaccaactg agctaagccg gctcatttgt tatatcttaa tgcgggttaa
    72001 gggacttgaa cccccacgcc gttaagcgcc agatcctaaa tctggtgcgt ctgccaattc
    72061 cgccaaaccc gcatatatga cccgtactgg gctcgaacca gtgacccatt gattaaaagt
    72121 caattgctct accaactgag ctaacgagtc taaaataact tgcgttacct taaacggtcc
    72181 cgacgggaat cgaacccgcg atcttcgccg tgacagggcg acgtgataac cgctacacta
    72241 cgggaccttt gggagttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct
    72301 ctcccagctg agctaaactc cctagagcta agcgacttcc ctatctcaca gggggcaacc
    72361 cccaactact tccggcgttc tagggcttaa cttctgtgtt cggcatgggt acaggtgtat
    72421 ctcctaggct atcgtcactt aactctgagt aatacctact caaaattgaa tatctattca
    72481 acttaagaaa accgttcgct ttcatattct cagttacttt ggataagtcc tcgagctatt
    72541 agtattagtc cgctacatgt gtcgccacac ttccacttct aacctatcta cctgatcatc
    72601 t
//
LOCUS       DAYYQN010000003        62338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYYQN010000003 DAYYQN010000000
VERSION     DAYYQN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.003"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            56..340
                     /locus_tag="ACHBRQ_000165"
     CDS             56..340
                     /locus_tag="ACHBRQ_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591909.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            667..885
                     /locus_tag="ACHBRQ_000166"
     CDS             667..885
                     /locus_tag="ACHBRQ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591910.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(1252..3576)
                     /gene="pflB"
                     /locus_tag="ACHBRQ_000167"
     CDS             complement(1252..3576)
                     /gene="pflB"
                     /locus_tag="ACHBRQ_000167"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9591911.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            3924..4985
                     /gene="dinB"
                     /locus_tag="ACHBRQ_000168"
     CDS             3924..4985
                     /gene="dinB"
                     /locus_tag="ACHBRQ_000168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9591912.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(5132..5977)
                     /locus_tag="ACHBRQ_000169"
     CDS             complement(5132..5977)
                     /locus_tag="ACHBRQ_000169"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9591913.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            6233..7101
                     /locus_tag="ACHBRQ_000170"
                     /pseudo
     CDS             6233..7101
                     /locus_tag="ACHBRQ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7402..9291)
                     /locus_tag="ACHBRQ_000171"
     CDS             complement(7402..9291)
                     /locus_tag="ACHBRQ_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFM9591914.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            9429..10994
                     /locus_tag="ACHBRQ_000172"
     CDS             9429..10994
                     /locus_tag="ACHBRQ_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9591915.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            10994..11734
                     /locus_tag="ACHBRQ_000173"
     CDS             10994..11734
                     /locus_tag="ACHBRQ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9591916.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(11843..12058)
                     /locus_tag="ACHBRQ_000174"
     CDS             complement(11843..12058)
                     /locus_tag="ACHBRQ_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591917.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(12929..14179)
                     /gene="ilvA"
                     /locus_tag="ACHBRQ_000175"
     CDS             complement(12929..14179)
                     /gene="ilvA"
                     /locus_tag="ACHBRQ_000175"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HFM9591918.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(14229..14585)
                     /locus_tag="ACHBRQ_000176"
     CDS             complement(14229..14585)
                     /locus_tag="ACHBRQ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFM9591919.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(14823..15059)
                     /locus_tag="ACHBRQ_000177"
     CDS             complement(14823..15059)
                     /locus_tag="ACHBRQ_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591920.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(15166..16188)
                     /gene="ilvC"
                     /locus_tag="ACHBRQ_000178"
     CDS             complement(15166..16188)
                     /gene="ilvC"
                     /locus_tag="ACHBRQ_000178"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFM9591921.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(16254..16730)
                     /gene="ilvN"
                     /locus_tag="ACHBRQ_000179"
     CDS             complement(16254..16730)
                     /gene="ilvN"
                     /locus_tag="ACHBRQ_000179"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFM9591922.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(16723..18423)
                     /locus_tag="ACHBRQ_000180"
     CDS             complement(16723..18423)
                     /locus_tag="ACHBRQ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFM9591923.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(18623..20290)
                     /locus_tag="ACHBRQ_000181"
     CDS             complement(18623..20290)
                     /locus_tag="ACHBRQ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9591924.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(20293..20658)
                     /locus_tag="ACHBRQ_000182"
     CDS             complement(20293..20658)
                     /locus_tag="ACHBRQ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9591925.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(20815..21003)
                     /gene="rpmB"
                     /locus_tag="ACHBRQ_000183"
     CDS             complement(20815..21003)
                     /gene="rpmB"
                     /locus_tag="ACHBRQ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9591926.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(21119..21295)
                     /locus_tag="ACHBRQ_000184"
     CDS             complement(21119..21295)
                     /locus_tag="ACHBRQ_000184"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9591927.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAISLAAVRDDFYTHNFNSGILLIN
                     NGMWRAENVTQDLI"
     gene            complement(21406..21603)
                     /locus_tag="ACHBRQ_000185"
     CDS             complement(21406..21603)
                     /locus_tag="ACHBRQ_000185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591928.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(21857..23401)
                     /locus_tag="ACHBRQ_000186"
     CDS             complement(21857..23401)
                     /locus_tag="ACHBRQ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9591929.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23614..23916
                     /gene="gatC"
                     /locus_tag="ACHBRQ_000187"
     CDS             23614..23916
                     /gene="gatC"
                     /locus_tag="ACHBRQ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9591930.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            23916..25382
                     /gene="gatA"
                     /locus_tag="ACHBRQ_000188"
     CDS             23916..25382
                     /gene="gatA"
                     /locus_tag="ACHBRQ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9591931.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            25382..26824
                     /gene="gatB"
                     /locus_tag="ACHBRQ_000189"
     CDS             25382..26824
                     /gene="gatB"
                     /locus_tag="ACHBRQ_000189"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9591932.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            26953..27513
                     /gene="efp"
                     /locus_tag="ACHBRQ_000190"
     CDS             26953..27513
                     /gene="efp"
                     /locus_tag="ACHBRQ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9591933.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            27535..27924
                     /locus_tag="ACHBRQ_000191"
     CDS             27535..27924
                     /locus_tag="ACHBRQ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9591934.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            27917..28339
                     /gene="nusB"
                     /locus_tag="ACHBRQ_000192"
     CDS             27917..28339
                     /gene="nusB"
                     /locus_tag="ACHBRQ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9591935.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(28386..28622)
                     /locus_tag="ACHBRQ_000193"
     CDS             complement(28386..28622)
                     /locus_tag="ACHBRQ_000193"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9591936.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(28635..29108)
                     /locus_tag="ACHBRQ_000194"
     CDS             complement(28635..29108)
                     /locus_tag="ACHBRQ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591937.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(29126..29311)
                     /gene="briC"
                     /locus_tag="ACHBRQ_000195"
     CDS             complement(29126..29311)
                     /gene="briC"
                     /locus_tag="ACHBRQ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HFM9591938.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(29470..30237)
                     /locus_tag="ACHBRQ_000196"
     CDS             complement(29470..30237)
                     /locus_tag="ACHBRQ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9591939.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(30234..31100)
                     /gene="accD"
                     /locus_tag="ACHBRQ_000197"
     CDS             complement(30234..31100)
                     /gene="accD"
                     /locus_tag="ACHBRQ_000197"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9591940.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(31137..32504)
                     /gene="accC"
                     /locus_tag="ACHBRQ_000198"
     CDS             complement(31137..32504)
                     /gene="accC"
                     /locus_tag="ACHBRQ_000198"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9591941.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGYVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(32516..32938)
                     /gene="fabZ"
                     /locus_tag="ACHBRQ_000199"
     CDS             complement(32516..32938)
                     /gene="fabZ"
                     /locus_tag="ACHBRQ_000199"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9591942.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSDNTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(32935..33420)
                     /gene="accB"
                     /locus_tag="ACHBRQ_000200"
     CDS             complement(32935..33420)
                     /gene="accB"
                     /locus_tag="ACHBRQ_000200"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9591943.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(33423..34658)
                     /gene="fabF"
                     /locus_tag="ACHBRQ_000201"
     CDS             complement(33423..34658)
                     /gene="fabF"
                     /locus_tag="ACHBRQ_000201"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9591944.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEVNVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(34680..35411)
                     /gene="fabG"
                     /locus_tag="ACHBRQ_000202"
     CDS             complement(34680..35411)
                     /gene="fabG"
                     /locus_tag="ACHBRQ_000202"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9591945.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(35445..36365)
                     /gene="fabD"
                     /locus_tag="ACHBRQ_000203"
     CDS             complement(35445..36365)
                     /gene="fabD"
                     /locus_tag="ACHBRQ_000203"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9591946.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSNFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(36358..37332)
                     /gene="fabK"
                     /locus_tag="ACHBRQ_000204"
     CDS             complement(36358..37332)
                     /gene="fabK"
                     /locus_tag="ACHBRQ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFM9591947.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(37451..37675)
                     /locus_tag="ACHBRQ_000205"
     CDS             complement(37451..37675)
                     /locus_tag="ACHBRQ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9591948.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(37735..38709)
                     /locus_tag="ACHBRQ_000206"
     CDS             complement(37735..38709)
                     /locus_tag="ACHBRQ_000206"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9591949.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(38709..39143)
                     /locus_tag="ACHBRQ_000207"
     CDS             complement(38709..39143)
                     /locus_tag="ACHBRQ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9591950.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(39237..40022)
                     /locus_tag="ACHBRQ_000208"
     CDS             complement(39237..40022)
                     /locus_tag="ACHBRQ_000208"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HFM9591951.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            complement(40082..40204)
                     /locus_tag="ACHBRQ_000209"
     CDS             complement(40082..40204)
                     /locus_tag="ACHBRQ_000209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9591952.1"
                     /translation="MKIKEQTRKLAADCSKHCFEVVNKTDEVSSKYCFEVADGS"
     gene            40392..41756
                     /locus_tag="ACHBRQ_000210"
     CDS             40392..41756
                     /locus_tag="ACHBRQ_000210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9591953.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            41759..42130
                     /locus_tag="ACHBRQ_000211"
     CDS             41759..42130
                     /locus_tag="ACHBRQ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9591954.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            42344..43618
                     /gene="serS"
                     /locus_tag="ACHBRQ_000212"
     CDS             42344..43618
                     /gene="serS"
                     /locus_tag="ACHBRQ_000212"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9591955.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(43643..44542)
                     /locus_tag="ACHBRQ_000213"
     CDS             complement(43643..44542)
                     /locus_tag="ACHBRQ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9591956.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDMLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPERRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(44652..45200)
                     /locus_tag="ACHBRQ_000214"
     CDS             complement(44652..45200)
                     /locus_tag="ACHBRQ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9591957.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(45418..46740)
                     /locus_tag="ACHBRQ_000215"
     CDS             complement(45418..46740)
                     /locus_tag="ACHBRQ_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFM9591958.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSYFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(46831..46919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(47083..49419)
                     /locus_tag="ACHBRQ_000216"
     CDS             complement(47083..49419)
                     /locus_tag="ACHBRQ_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9591959.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFSDIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(49523..50071)
                     /locus_tag="ACHBRQ_000217"
     CDS             complement(49523..50071)
                     /locus_tag="ACHBRQ_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM9591960.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(50068..50370)
                     /gene="zapA"
                     /locus_tag="ACHBRQ_000218"
     CDS             complement(50068..50370)
                     /gene="zapA"
                     /locus_tag="ACHBRQ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9591961.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            50457..51338
                     /gene="rnhC"
                     /locus_tag="ACHBRQ_000219"
     CDS             50457..51338
                     /gene="rnhC"
                     /locus_tag="ACHBRQ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9591962.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            51342..51956
                     /gene="lepB"
                     /locus_tag="ACHBRQ_000220"
     CDS             51342..51956
                     /gene="lepB"
                     /locus_tag="ACHBRQ_000220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9591963.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            52084..54450
                     /locus_tag="ACHBRQ_000221"
     CDS             52084..54450
                     /locus_tag="ACHBRQ_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9591964.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGITGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDAEFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(54498..55781)
                     /gene="tig"
                     /locus_tag="ACHBRQ_000222"
     CDS             complement(54498..55781)
                     /gene="tig"
                     /locus_tag="ACHBRQ_000222"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9591965.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLTELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(56587..57723)
                     /locus_tag="ACHBRQ_000223"
     CDS             complement(56587..57723)
                     /locus_tag="ACHBRQ_000223"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9591966.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(57785..58222)
                     /locus_tag="ACHBRQ_000224"
     CDS             complement(57785..58222)
                     /locus_tag="ACHBRQ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9591967.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(58224..60179)
                     /locus_tag="ACHBRQ_000225"
     CDS             complement(58224..60179)
                     /locus_tag="ACHBRQ_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9591968.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKLEEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(60203..61972)
                     /locus_tag="ACHBRQ_000226"
     CDS             complement(60203..61972)
                     /locus_tag="ACHBRQ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9591969.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATKAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            62181..>62338
                     /locus_tag="ACHBRQ_000227"
     CDS             62181..>62338
                     /locus_tag="ACHBRQ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9591970.1"
                     /translation="MKLTYDDKVQIYELRKQGYILEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKG"
BASE COUNT        18602 a        13858 c        11672 g        18206 t
ORIGIN      
        1 acattttcga atataaacta caataatata aactaaattt tataggagga agacaatgga
       61 ttggtacgat tatatgatac aggcatccaa acaatcacaa ttcaacgcaa gccattggtt
      121 tcgctatttg cgaaaagtta tttttgaaga ctattcttat ttaacaaacc aagatgtaga
      181 aaagttgcta gactccaaag aactaacccg ttttcaaaaa attagcttga agtatgcctt
      241 tcaagagcat actccaactc ataaatatgt gatttcatta aataaacctg ctaagttaac
      301 caatgttcaa aaattgatgg agaaatacaa acatggataa aatgaaaccg gtcttccaag
      361 ccctaaataa gaaattaatt caggaaaatc tgactttaac aattatggtt atgtcttaga
      421 atatcatggt ttacgtgcca cacaagatgt tgatgctttt tatgaccaaa atcagaaaat
      481 caatgaaatt attgctcgtg taggaaaaca gtttaatctt aacatccacg aagagttatg
      541 gctcaacaat catgttgcaa agcaaatatg aacaaacaac ctcccttaag tttatgcgaa
      601 tccttgtact catttgagaa tctgaccgtt ttagtagtcc ctattgagta tgtgttagga
      661 atgaagatga tgagtatcag agagcaggat ttgaaagata ttggtgcaat tattaaatac
      721 aaaaactttc attcaccatt cgataccttt aaatatttaa aagacatggg atttgatact
      781 attgacctct ctgttctatt agaaggtttt agttatgctt atggtatgga ttggttagaa
      841 aaatttttta aagaaaatca agataaactt cgcgaatttt attaacagta tcaaacaaga
      901 tgattgtcaa ttagccagtc gtcttatttt tatgatggaa tgaaataaaa tcggaacaaa
      961 tcacttagag aattccagtc aatttctagc gctgtttgag acgtttcagt ggactatagt
     1021 aaaatgaaat aagaatagga caaatcgatc aggacagtca aatcgatttc taagaatgtt
     1081 ttagaagtaa aggtgtacta ttctagtttc aatctattat atttcagatt cggtccacta
     1141 ttccttccca aaaacattgc atattgcgcc gaaggaattg ctcacactat cccaagcaca
     1201 aaaccctcag tcgtttgacc gagggttcct ttttattatt caagaacttg attagctcaa
     1261 tgcatccaag gcgtcatcca ttgaaagaac ttcgtggaag acacgttgtg tcaattcagt
     1321 tttttgttct ggagtgaggt atttagtgtt tacacagtat ccagagatac gtacgataac
     1381 gtcttcgcct gacatgattt tttcgtaaac atcgttcaag tccataacgt tcaagttaac
     1441 gtgttgtcca ccgttttcga agtaaccatc aaggattgtt accaagttat caacttgttc
     1501 atcacgagtc ttaccaagag cacgaggtga tacttgtgta gtcaatgaga taccgtcagc
     1561 tgcataacta aagtcaaggc tagaaagtga gttcaagttt tgcaaccaac cacctttagc
     1621 tttgttagat gggttagcac ctggtgagaa gaattcaagt ttagacaagt tcacagaacc
     1681 atcttcgttg aggtatacac ctttgtgaac tggtgagtta ccagtttgtt tagagtaagc
     1741 aacgttagat gtgattgtca aaagtgatac tgtagcttct gcgtctttgt atagtttgtg
     1801 actacgtaga cgagttgtgt aagcttcgat caaccattct gccaattcgt ttgaacgtgg
     1861 gtcatcttca ccccagcgtg ggtagtcacc gattgtttcg taatcgtaga tgtagccatc
     1921 ttcgtcacgg attggtttaa ctgtagcgta tttgatagct gacaatgtat caacagtgtt
     1981 agcaaatcca cagataccga atcccatgtt ggcacgttgt ttagttggca agaaggccat
     2041 ttgaacagct tcgtagttgt acctatcagt catgtagtgg atgatgttca aggcatctac
     2101 gtaagtgtca gtcaaccagt caagagattt ttcaaagttc gctttaactg attcgaattc
     2161 aagaacttcg tcacggattg gttcgatatc aaatactttg tagtctttgt gaacatcgtc
     2221 gtaaccacca ttcaaaccag taagaagggc tttaagaacg tttacacgag caccgaagta
     2281 ctggatgttg tggcgttgtt cttcattttc tggatcaagt ggagacacac agcatgagat
     2341 acagctcatt tcaccatatc cgtctttagc cattgttgtt acaccttcgt attggataga
     2401 agagtgtttg tggctcatgt gcatacagta gcgacggaag ttgtatggca atttgtcagt
     2461 ccaaagaact gtcaagtttg gttctggtga gttaccgatg ttgtcaagag tgttcaagaa
     2521 acggtagtcc atcttagtaa cacggtgacg accgtcgtta cccataccag ccatagaagt
     2581 tgtgataaag gttgggtcac ctgagtacaa ttggtcataa gcttttgtac gagcaaattt
     2641 aactgtacga agtttcataa cgaaatcatc aacgaattct tggatttctg attcagtaaa
     2701 tgtaccacga gcaaggtcac gttctgcaaa gatgtccaat acgattggta cacgacctag
     2761 agatgtagca gcaccgttaa tcacacggca gacagccatg aaagcaatgt taacccattg
     2821 gattgcttct ttcacgttca tcgctggttt gcgaacatca accccgtaaa ggtcacccag
     2881 gcgaacaact tgttgcaatg cttggtattg aaggtttact tcttcacgaa gacggattgt
     2941 ttcttcatcg atttctttga ttgcattcca gtcgtttact ttttcttgca tcaagtagtc
     3001 tgcaccgtaa agagcaagac gtgcgtaaac accgatgata cgtccgcgtg agtatgcatc
     3061 tggaagacca gttacagtgt gtgcgtgacg agcgcgacga atatttgaag tgtaggcacg
     3121 gaaaataccg tcgttaactg ttgttacata tttagtgaag atttcgtgaa cagctgggtc
     3181 tggttcgtat ccattttctt tcaaagtagt ttcagccata cggataccac cttttggcat
     3241 gaagttcaat ttgaagagtt catcgttttg gataccgaag ataacttcat tttctttgtc
     3301 gataaatcca gcagggatat cagcgataga tgttggacga gtgtccattg ggaaacgagt
     3361 ttcttcgtag tgtgctttag tttcttctac aattttcttg atgtgaagtg aacgctctgt
     3421 tggtcctgca aggaagcttt cgtctccatc ataaggtgtg tagttagctt gtacaaatcg
     3481 tgatacactt gctttttctt tccaatctac gcctttgaag ccttcccaag ctttgtcaaa
     3541 aatatcttgt gcttcaacaa ctgtcttaac aaccatatta atgtcctctt ttttctagta
     3601 acagtcatct gttacattca taagacaagt ataccatata gtaaccgatt tcaacaagtg
     3661 aaaattccct atttttacac tttcttttct aaaacagtct atattttatt tcaaactgta
     3721 ttatattttt gaaaaaataa agtccttttt tctttttttc agaaaaaagg gtataataaa
     3781 agaaaataag cagtaacact caatggaaat cgaaaagcaa actaggaagc tagccgcaga
     3841 ttgctcaaaa cactgttttg aggttgcaga tagagctgac gtggtttgaa gagattttcg
     3901 aagagtataa aaaggtgcta ggcatgttga tttttccttt gttaaatgat ttgtcaagaa
     3961 aaatcatcca tattgacatg gatgcctttt ttgctgcagt ggaaatcagg gataatccta
     4021 aactcagagg aaaacctgtc attattggaa gcgaccctcg gcaaacaggt ggacggggag
     4081 tcgtttctac ctgtagttat gaggcaagag cttttggtgt ccattctgcc atgagttcca
     4141 aggaagctta tgaacgttgt ccccaggctg tctttatctc agggaattat gagaaataca
     4201 agtctgtggg actccagatt cgagctattt ttaagcgcta tacagatttg attgaaccca
     4261 tgagcattga cgaagcctat ttggatgtga cagaaaataa actcggtatc aagtcagcgg
     4321 tcaaaattgc tcgcctcatt caaaaagata tctggcaaga actccatcta actgcttccg
     4381 caggcgtttc ttacaacaaa ttcttagcta aaatggcgag tgattatcaa aaaccacatg
     4441 gtttgacagt gattctacct gaacaggctg aggattttct caaacaaatg gatatttcca
     4501 aatttcatgg agtaggaaaa aagacagtag aacgtcttca tcaaatgggc gtttttactg
     4561 gtgctgattt acttgaagtt cctgaggtaa ccctaataga ccgttttggt agactaggct
     4621 atgatctgta tcgaaaggct cgtggcattc acaactctcc agtcaaatcc aatcgcatcc
     4681 gtaaatcaat cggcaaggag aaaacctacg ggaagattct ccttgctgag gaagatatca
     4741 aaaaagagct gactcttcta tcagaaaaag tcgctctcaa tctacatcaa caagaaaaag
     4801 ctggaaaaat tgtcattttg aaaatccgct acgaggactt ttcaactctt accaaacgaa
     4861 aaagtcttgc tcaaaaaaca caagatgcta gtcagataag ccaaatagcc ctgcaactct
     4921 atgaagaatt aagcaagaaa gaaagaggtg tccgcctatt ggggattacc atgactggat
     4981 tttaaagctc aatgaaaatc aaagagcaaa ctaggaggct agccgcaggc tgctcaaaac
     5041 actgttttga ggttgcagat aaagctgacg cggtttgaag agattttcga agagtataaa
     5101 accttgaaga gttgcccctt caaggttttt cttatacaaa taaacggaca aaactgtaaa
     5161 gtagcaaaac actaccaagc acgatacggt atttaccaaa aagggtgaag tcgtgttttt
     5221 tcacatagct ggtcaagaag cgaatagcca ccatgctgac cgcaaaagct actcccatcg
     5281 cgaccaagag taaaaacaat tgcccaaagc tcaagagttc tccggctttc acaaatttga
     5341 aaatctttaa ggcactagct ccaaacataa cgggaatccc aagatagaag gtaaattctg
     5401 tcacaactga acgactggtt ccatttaaca aaccaccgac aatcgttgca cctgaacggc
     5461 tagtccctgg taaaagagca agaacttgga agagtccgat atagaaagcg gtcgtataag
     5521 gaagcttgtc caactctgtt acacttggct cgatagcacg cgctttattg cgcttttcca
     5581 aatatatgaa ggcaaccccg tagataatca acatgagagc aactgaaacc atgttatgga
     5641 agtgggtatc aaaccaatca tcaaatttaa agacaccaag taaaggtaaa gtagcaatca
     5701 agaccttcaa ccatagtctc caagtcttac gaacttcctg tttgtcctta gtcggtttaa
     5761 aaggattgag cttgttaaaa taaatcacca taactgctaa aatagcacca agctgaatca
     5821 cgacattaaa catggacata aaggcttcat tttgattttg gtattggata aactcctctg
     5881 ctaaaatcaa gtgacctgta ctggaaatcg gcaaccattc cgtaattcct tcaacaatcc
     5941 cgaagaagat agattttaaa atttcaataa gatacatata tttctcctta tattatattt
     6001 aacgcagtta gctactgcca aagcccaagt ggtatacttg gaataagcca ctgtggatta
     6061 gttcatttcc tccaatcgct tgacgatttc cgaaagggtc taacaacagc tttttgtttc
     6121 aattggtaat gagtttgata acgcatagac gttttatctc gtctatggta acaaggcaaa
     6181 aagttcgtgg ataaccaagt agacttgaaa tactatatct ggaggtactc ccatgcttta
     6241 tgtgggcatt gatgtcgcta aaaataaaca cgatgttaca gccttgaatg ttcgaggaaa
     6301 actgttctta aaccactcac tttttcaaat aataaagctg gttttgaact cttagatctg
     6361 tcttttcgac agctcaacca agactgtctc atcgctctta aacttctttc tgaccccaat
     6421 cgtgaacaat ttcaacacga taatcggcaa atagaactaa aaatactggc tagacatatt
     6481 catcgtctca agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc
     6541 atctttcctg agttggataa aatcgttgga aagcattcag aatataccta ccaactcttg
     6601 acacgctacc ctaatcctca gaaaaggctt gaggcaggat ttgataagct gatagaaatt
     6661 aagcgattga ccgcttctaa aattcaggat atcctctcag tcgcacctcg ttctatcgga
     6721 acaacatctc ctgctcgtga attcgaaatc atcgaaatca tcaaacatta caagaggctc
     6781 attgacaagg cggaaacatg tgtcaatgac ttgatggctg agttcaactc ggtcatcacg
     6841 acggttactg ggattggggg tcgtttaggg gcggtcattt tagccgagat tcgaaatatt
     6901 catgcctttg ataatcctgc tcaattacaa gctttcgctg gactggattc ttctatttat
     6961 cagtcaggtc agattgattt agctggaaga atgatcaaac ggggttcccc tcatctgcgg
     7021 tgggcactca tacaagctgc caaagcatgc gctcgctttt cacctgcttt taaggcctat
     7081 cttaagacta agttagaata aggaaaacat tacaatgtag ccatcatcca ccttgcaaaa
     7141 aaacttatcc gaaccctgtt ttatatccta aaaaagagct gccatttgac gaacaaaaag
     7201 tgatgtaatt tctgaaatta acacaggatt acctttgaaa aaatggactt tttcaagggc
     7261 tttttgccgt gctctaaaat catcgccaaa tcatcaaatt gaaataagaa atagtttaga
     7321 taaaactatt gacttttctt atagtttgtc tttcttctat cttccattat atcatacttt
     7381 ttcagtctat ggtagctctc tttaaaaggc tccgatactg ccgccacctc cgcctccaga
     7441 gaagccacca ccagaacttc cacttccaga agatacagag taggtacttg ctgtatttgc
     7501 gacactagca taatggctca tttgcgcgct tgaatgataa aacatactgt gccagccata
     7561 agctacatag agattgatat ctggattttc aacttgaatc tgatgaacct tcatcaaatg
     7621 actaaccttg tccgcatagc caaataaggt tgcatagacc aagagacgat tccaaacaac
     7681 aatactttcc aattcagcct tatccagtcg tgcaatctca cgtaacatat tttcaaaact
     7741 ggtccagaga tagtagacct cagctcccgc ttcatttaga acaccatcac gattatctag
     7801 tcgaagcttc caataataga aaacagacaa aactaaccct agaaaaccaa gtattggcaa
     7861 agggaggtaa agatagccat gaacgtctaa actgtacaag aacaaaccaa atccgataaa
     7921 taggggcaag atagtcaaga cacccatacc cacttgcaaa gccttttcca aaccagttaa
     7981 aggacgatag taatctggga gtccccagaa gacaactcgc tttctcactc cttcttgcat
     8041 ctcttttaat actgcttcga aagaagattt gagctgaatc cctcttgctt gaatccgttt
     8101 ctcatcagca gctttagctc cgcgataaag gctgtcagat accttgtaat ccgcaaacaa
     8161 attggaaaga gtggcttctt ttttgcctga aaaggctaga tttagacagt ctctctcaaa
     8221 tcttgacaaa ccatcttctt tcaccaatct caagccaact gcatctcctt ctgaaataat
     8281 agaaacattc ccacggtcca tcacatctag caaggtagct tgaataagtt ggtcaaaagt
     8341 gaatttgcca gcccccttga ctaagggact cacttcctcc aaggaggtcg agtagacagc
     8401 ctctgataaa accataggct ctaattccat tggtggttca tagagacgat gatttttggc
     8461 atatttgact gaaggagtgg tctttcttct ataaataaaa tagaagcaga cactcaataa
     8521 caaggagata gaaaggatcg aagggaggac ccaagtaaca agttgtttac tctgatcttt
     8581 ttctctaaca atcgagtctt ctatcttatt aaactcttct aaacgattcc ctttcaatcc
     8641 ctgatcccta gcgctagcaa aatcggtccg aggccagtag gcatgcaact caactccacg
     8701 cttagccgga agattgtcta aacggatagt ataatcaagg ttactctttt caatcgttcc
     8761 ctctctaaaa agtttccctg tatggaaaaa gagtttttca gcccccttgt ctccccttac
     8821 atgaaattca aacttttcaa tagactctga actatctgtc agaggttgcc aatttaattc
     8881 agcgatatca tcataaagga aaagtaaatt ttttaagttc cagacgaggt caacttcaac
     8941 tatgtcgccc tcctgacctg gattatagac tctcacagta taaccatccg cttcttctgt
     9001 tacttcgcta gtcacatctg ctagttctgc accgtttttc gcggcctgaa cctttggatg
     9061 agggtcaatg tcaaacccgc taggcatctt accagcacgt ccaagtccca cgatttgtcc
     9121 cttaaagtcc tcctcaaact ggtaaactat cttctgtcta aactctgccg tattatctgc
     9181 atgaatatac aaatcccctt gataagagtt tatcttgaaa tcaatggcaa aaacagagag
     9241 tggaagaagg caaaacaagc ctaacaccag taagaaaaaa gtttttttca tcaactaagc
     9301 ccccttttta tctttcttat cattatagca ttttttcgta agtaaaggct tacttatgtt
     9361 gaaaaattac gcatttttcg ataaaatagg agacagttat tttttaatag atcagaatag
     9421 gagaaatcat gagaaaaata tacttatcta ttttcacaag tctcttgctg atgctaggac
     9481 ttgtcaatgt tgctcaagcc gatgaatatt tacgcatcgg gatggaagca gcatatgctc
     9541 cctttaactg gacccaggat gatgatagca acggagctgt caaaatcgat gggaccaatc
     9601 agtatgccaa cggatacgat gttcaaatcg ccaagaaaat cgctaaggac ttaggtaaag
     9661 aacctttggt tgttaaaacc aagtgggaag gtctagtccc tgcccttact tctggtaaga
     9721 ttgacatgat tatcgcaggt atgagtccaa ctgcagaacg caaacaagaa attgcctttt
     9781 cgagcagtta ctacactagc gaaccagttt tgcttgtcaa aaaagattct gcctacgcaa
     9841 gtgctaaatc tttggatgac tttaatggtg cgaaaatcac ttctcaacaa ggtgtctacc
     9901 tctacgactt gattgcacaa atcccaggtg ctaaaaaaga aacagccatg ggagacttcg
     9961 ctcaaatgcg ccaagctctt gaggctggtg tcattgatgc ttatgtttct gaacgtccag
    10021 aagcactgac tgctgaagct gcgaactcta agttcaagat gattcaagta gaacctggtt
    10081 tcaaaactgg ggaagaagat acagctatcg ctatcgggct tcgtaaaaat gacaatcgta
    10141 ttagccaaat caatgccagc attgaaacca tttcaaaaga tgaccaagtt gccttgatgg
    10201 atcgtatgat caaggaacaa cctgccgaag ctacaacaac tgaagagact agcagtagtt
    10261 tctttagcca agtcgctaaa attctttctg aaaactggca acaactcttg cgtggtgctg
    10321 gtatcactct tttaatctct atcgtcggaa ccatcatagg tctcattatt ggacttgcca
    10381 ttggtgtctt ccgtactgct cctctctctg aaaacaaagc catttatggc cttcaaaaac
    10441 tagtcggctg ggttctcaat gtctacattg aaattttccg tggtacgcca atgattgttc
    10501 aatcgatggt tatctactat ggaactgccc aagctttcgg gatcaacctt gaccgtacac
    10561 tggctgctat cttcatcgtt tcaatcaata ccggtgccta catgactgaa atcgtccgtg
    10621 gtggtatcct agcagttgac aagggacaat ttgaagctgc gactgctctt ggtatgaccc
    10681 ataaccagac catgcgtaag attgtcctac ctcaggtagt ccgtaacatc ctacctgcaa
    10741 ctggtaatga atttgtcatc aatatcaaag atacatctgt attgaacgtt atctctgttg
    10801 tcgaacttta tttctcagga aataccgtgg caacacaaac ctatcaatac ttccagacat
    10861 ttacaatcat cgccgtgatt tactttgtcc tcaccttcac cgtaacacgt atcctacgat
    10921 ttatcgagcg ccgaatggac atggatacct atactacagg tgctaaccaa atgcaaacgg
    10981 aggatttgaa ataatgacac aagcaatcct tgaaattaaa cacctcaaaa aatcctatgg
    11041 acaaaacgaa gtgctaaaag acatttcact cactgtccac aagggagagg tcatctctat
    11101 catcggaagc tctggaagcg gaaaatcgac cttcctacgc tccattaacc tacttgaaac
    11161 accaactgat ggacaaatcc tttatcatgg acaaaacgtc ctcgaaaaag gctatgacct
    11221 cacgcaatac cgtgaaaagt tggggatggt tttccaatcc tttaacctct ttgaaaatct
    11281 caatgttctt gaaaacacaa tcgtcgctca gacaactgtc ctaaaacgcg aacgcacaga
    11341 agctgaaaag attgccaaag aaaacctgga aaaggtcggc atgggagaac gctactggca
    11401 agccaaacca aaacaactct caggtggtca aaaacaacgt gtggccatcg ctcgtgccct
    11461 ctccatgaat ccggacgcta ttctctttga tgaaccaaca tcagctctcg atccagaaat
    11521 ggttggagaa gtcctcaaaa tcatgcagga cctggctcag gaaggcttga ccatgattgt
    11581 cgtaacccat gaaatggaat ttgcccgtga tgtctctcac cgtgttatct ttatggataa
    11641 gggcgtgatc gctgaagaag gtaaaccaga agacctcttc accaatccta aagaagaccg
    11701 aacaaaagag ttccttcaac gctatctcaa ataaaaagaa aaggctgcat caaccgtgca
    11761 gtctttttgc tgtcctcaaa atgaaaaggc agattctcta tctctactac ttgaaggtca
    11821 agaatccgcc gttatccaaa gattaatctt caaatttttc ataatttttt tcatagaaat
    11881 caattaggcc tagggctgtt tcaaatgaaa tattttttac ttttgcacga ccctgcgcca
    11941 gagcaatgat agacatttca cgcgcattcg tttctttaga gatacggtag cctgtgattt
    12001 tcttatcacg aacccagcca acaactgatt ctactttttc aaagtttgac ttagccatgt
    12061 ttttctccta ttttcttctt tataatattt aattgtatac gtttttacta cttttgtcaa
    12121 taaaaaaaac atatattcaa cgaaaataat tagttcagaa ttttcttact tcctttttaa
    12181 atccgataat atataggttt tgctagcctc tacaccataa atatttgcta ataaaactac
    12241 tgtattatta ccctcttaag gtgcgaaatt attcaagttc ttaaaaaatg cttctctatt
    12301 tccgtaagat agcttatttc ttcgatttaa gaactgaaga tgatagttct aacctttaga
    12361 aatccaagcc ttcactagct caaaaactcc tatctttctt cctcaataaa tcatctgaga
    12421 gataggtatt ccttatatcc ttcaaattca tcactccttt ggatattgag gtgctagcct
    12481 agaagcagac acccgattat ctaaaccatc cttaatagag atgacaagac agataagctc
    12541 agttaattac ttatatatct ttttaatatg gtatatttgt caacaaaaag aaatcaaatc
    12601 ataccttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca agttgctcaa
    12661 aacactgttt tgaggttgca gatagagcta acgtggtttg aagagatttt cgaagagtat
    12721 taaagacagt gtgactatgc ttgtcaatag ataggaaatc aaaatgaagg atggaaatct
    12781 gagccaaaca tcataagtgc tataatgaac agaagtaaga attgcacgat aagttttcta
    12841 aaaccatagg aatcgtcccc tttcttcatc ttcattatag cacatataca tcagttgtac
    12901 aaataatatg atattttttt attagtcctc agacaagcat attataaagc gtttcattac
    12961 catttaagtt aatataagct ggatcaaaac cttccattct acgaatcaaa cctgcataat
    13021 catgcttatc tgctaaagcg atcccaatta atactgggcc tgttcccttg ctagctcgtt
    13081 tgatatactc aaaacgtgtg atatcatcat ttggccccag gatatcattt acaaactcac
    13141 gcaaagctcc tggacgttgt gggaaattga ccacaaagta atgtttgata ccatcataaa
    13201 tcaaggcacg ctcttccatt tctggcatac ggttgatatc attatttcct ccagaaatga
    13261 tacaacaaat ggttttcccc ttaatatatt cagctaaaac ctctaaagag gcgatactag
    13321 ccgctccagc aggttctgcg actatccctt gcttagagta aaggtcaatc aaggtttcag
    13381 aaatcaatcc ctcatcgaca cctaccaaag ttttaatatg ttgacgagtt gcttcatagg
    13441 tcaactgacc taccttttgc acagcaatcc catccgcaaa tttatcaatt tccttgagtt
    13501 tgactggacc tccagcctca aaggcagctt tcatggaacg cgctccattc gcctctactc
    13561 cgataacctc aatctctgga cttgtttcct tgatataggt agaaaccccg gcaatgagac
    13621 cgccaccacc aacaggaacc aagacagcat caaaatcaat cgattctttt cgagcttctt
    13681 ctaaaatctc ataagcaact gttccttgac ctgcttgaac atgagcatca tcaaaaggat
    13741 caataaaggt acgattttca gagactgtaa attcttgagc tgctttggct gaggcatcaa
    13801 aggtatctcc aactagttta atagttacaa aatccccacc aaaaaagcga acctgaccaa
    13861 tcttttgttg tggcgtagta atgggcataa agatagtagc aggaattttc atttcattac
    13921 aagtataggc tactccctgc gcatgatttc ccgcagaagc gcaaactacc ccacgttcac
    13981 gttcttcctt gctgagctgg gaaatggcat aataggcacc acgaatttta aaggagcgaa
    14041 cacgctgggc attttctttt ttcaaataaa tcttagcacc atacttctcc gataaataat
    14101 gatcgtaatc cagtggagta ttcacaacca caccgttcaa gaccttgtga gccttgatta
    14161 tatcttttga acttaacata taaaacctct ttttctataa aaaacagtca ctaatgactg
    14221 ttttttagtt attcatctcc gaaaatttca gtaacacttt catggtattt atgagccata
    14281 cgaatcgaaa actctttagg aagttcataa cgatttgtgg aaatccaatc atataaccat
    14341 tctaccaaac tttcttcact atcattacga tggaagataa aatagtttaa aatttcttta
    14401 tattctacat cattcaaatc caacgtgtct acaaattgac tacgaggaac aaaattagaa
    14461 tctaattttc ttagttgatc tgtcaattct cccaattgta aatccaactg aaaaaatagt
    14521 ttttgcagtt gcctatcagt ttcatcacat cctgtcttga tagaaaacaa attggtaata
    14581 ttcatatgat ttcaacccct aaataagtac atctaatatc ccacgaatag ctgaagctaa
    14641 agttttcgca gtccagtcat ttaaacccga ttctttcaat ccagatgcaa taatttgctc
    14701 tatttgatat aaaccgccat ctatcgtctc atttattctg cgaagaggac cttcaatata
    14761 ggataaagct tctccgaatc taacagctga tttaggtcct aatgatttac caactatatt
    14821 tgttaatatt ttttatttct caaaatccat ttcgctatat aacgagcaga tttagtcgca
    14881 ccagctctct caaacggaga atgaatttca agaccttctt ctctagctat ttgtgccaat
    14941 ttgaagaatt gttcaaaggt catatgcaac ctaccatctt caaaattagc atcatcataa
    15001 atgttaatta cagcattttc atcatacgac caatctgctt tattttggtc tgctagcact
    15061 attaccgacg gtgtaattac attatacaac tagaaccaat aataacgatg caatttttct
    15121 cataatcttt ttcataaaat catctccagc gctatatatt tctaattagt tatagatttt
    15181 gaatgcatca tcgtcgtttt taccaacgaa tggcattgct ttacgcaatt ctgcaccaac
    15241 tttttcaatt tcaaggttag ctgcttgttc acggtaagca gtcaattttg gacgtccagc
    15301 tttatagtca tttacaaagt catttgcaaa tttaccattt tggatgtctg ccaagacagc
    15361 cttcatattt tctttaactt gttcagtgat tacacgtgga cctgatacat agtcaccgta
    15421 ttcagcagtg tttgaaatag attgacgcat tttcttgaat ccaccttcgt agatcaagtc
    15481 aacgatcaat ttcatttcgt gaagaacttc aaagtaagcc aattctggag cgtaacctgc
    15541 ttctgtcaag acttcgaaac ctgcttcgat aagggcagtc aaaccaccac aaagtacagc
    15601 ttgttcacca aacaaatctt cttcagtttc ttctttgtaa gttgtttcaa gaagacctac
    15661 acgagccgct ccaacacctt tacaccagtc catagcaatg tttttagcat ttcctgttgc
    15721 atcttggtat actgcataaa gagctggaac accaaatcct tcttcgtaag tacgacgtac
    15781 caagtgtcct ggtcctttag gagcacacat gaagacatct acatccgcag gaactttgat
    15841 aaattcaaag tggatgttga aaccatgggc aaatccaact gcgtttccag cttccaagtt
    15901 tggagcgatt tctgcttcgt acaattcttg ttgaatttcg tctggcgcca agatcatgat
    15961 aacatcagcc aacttagtag cttctgctac tgtgtaagta tcaaatccat cttcttttgc
    16021 tttatcaaaa gatttacctg gacgtacacc gataataacg tcacgacctg aatcacgcaa
    16081 gttttgagca tgcgcatgcc cttgtgaacc ataaccgata acggcgattt ttttaccgtc
    16141 aagtgctgct actttaacat ctttttcata ttccatttga actgtcatag cttttctctc
    16201 ttttctatta tttattgcct tttaagctgg tttaacaaat ttaagttgga tttttaatcg
    16261 cgggtaaatc cagttgcacc cgttcgagca atattgcgaa taccgtatgg gcgaatgact
    16321 cgcaataggg cttcgctctt ttctgcattt cccgtcatct gaatggtaat cgagcttggc
    16381 gctacgtcta ccactgttgc acggaaaggt tgaataatcg ctaaaatctc agctctcttc
    16441 tcagctggcg ctgacatctt aaccaaaatc acctcgcgct ccaaatgagg cttgtctgta
    16501 atatctcgaa tgcgaatcac atcaatctga cgattgagct gtttgatgat ttgctccact
    16561 tcatcatgag aagcaacatc aataataata gtgatacgcg atacattcgg atcttctgtt
    16621 gctccaacag agatgctttc aatattaacc tgacgacgag ataggacacc tgtaaagcga
    16681 ttgaggactc ctgaacgatt ttgtagtttt gctgttaaca ttctacgcat ggaactgcac
    16741 ccccaacatc tcatgattac tcttaccagc cggtaccatt ggtaacacct gttccttacg
    16801 agaaatatct acctcaatta gcataggaac atcctcagtg atgacttcaa ggtcttgagc
    16861 caaggtctca ggattgtcaa acttatagtt tttaatacca taagcctgcg ccatcaattg
    16921 gaaatcagga agggtatcaa agaccgactc tgatgttctg ccttcataga aggattcctg
    16981 ccactggcga accattccga gtgaatgatt gttcagcata accaccttga ttggcacctt
    17041 gtaaatattc aaaatagcca actcctggtt ggtcatttgg aaaccaccat ccccaacaaa
    17101 caagactact tccttatctg ggttagcaat tttagcaccg attgctgctg gaattccaaa
    17161 gcccattgtt cccaaaccac ctgaagtcac taactgacgt tcattttggt agggataata
    17221 ctgagctgtc cacatttggt gttgaccaac gtctgttacc acaatggcat ctccattcgt
    17281 caattcacca attcgttcaa taactgcttg cggttgaacc acacgctctt tcttgtcata
    17341 cgaacgaaca cgattcttgt ctttagtgac tttctcaatc cacttttcag tattgttgtg
    17401 aactgttggt tctgctagca acatttgcaa ggccttctta gcatctccaa ctacaggaat
    17461 gtctgcactg ataatcttgc caatctcagc tgggtcaata tcaatgtggg caaccttagc
    17521 attcttagcg aaagtcttag gattccccgt caaacggtca tcgaaacgag aaccaatact
    17581 aatcataaag tccgcttccg tcatggcaat atttgctgcg aatgacccgt gcatgcctcc
    17641 cattccaaga aagagtgggt gactcgttgc aatcgttcct tgtcccaaaa gactggttac
    17701 cactggaatt tgatagcgtt ctgcaaattc atttagttcc gtagcagcct cagcataact
    17761 aattccacca ccagctaaca agactggctt tttagccttg gacaattgct tcaagatttt
    17821 cttgatttgc atatcattcg gctcaagagt cggctgataa cttggtaaat tcacttctgg
    17881 tgaataaatg aagtctgttt ctaaagcaga tatgtctttt ggtaggtcaa ttacaactgg
    17941 ccctggacgg cctgtagttg cgatatggac agcttccgta atgatacgcg gaatatcagc
    18001 tgtctcacga acttggtaat tgtacttagt gattggcatg gtaattccta cgatgtctgc
    18061 ctcctgaaag gcatccttcc caatccctgc tcgcgccacc tgacctgtaa agaccaaaag
    18121 gggaacgcta tcgctcatag catccgcaat ccctgtaatg gcatttgttg ctcctggtcc
    18181 actagtgacg acggcaacac ccaactttcc agttgatttg gcatagcctt cagcttcatg
    18241 caaacaacct tgctcatggc gccctagaat gtggcgaatg cctttaaaat tatatatcgc
    18301 atcataaaaa ggcaaaaccg caccaccagg ataaccaaag atggtatcaa ctcctaaatc
    18361 acgaagtgtt tccaaaacta ggtccgaccc cgtcttagga gattctaaac tgattttctc
    18421 cattattccc ctttctttcc tcttaaaaat aacttgttac tatcatacca ttttttcaaa
    18481 atttttcaag ataaaagaag aaattttctg aattttctat tttaacgttt atttatgaat
    18541 gttatttttg tttttattaa aaagataccg atttcattta ctaaaaagaa aaaagaactg
    18601 atttctcagt ccttcattaa tcttattcca cactaaatag gtatgggtaa acaggttgtt
    18661 gaccttggtg aatctcgact tcaacgtctt cgaattcttc tacgatttct tgagcgattt
    18721 cattggcaag ttcttcgctt ccgtcttcac cgacatagaa ggttacgatt tcactgtctt
    18781 catccaacat atgtttcaag gtttcagtca atgtttggtg catatcaggg tttgacacaa
    18841 gaatttttcc atccaccata cctaaattat cgttttcatg gatttctaag ccatcgatcg
    18901 ttgtatcacg cacggctgtt gtgacgcttc cgctaacgac atcgctaaga gcagctgtca
    18961 tacgctcttg gttttcttca atggacttgc ttggatcaaa ggcaagaaga cttgtcatac
    19021 cttgaggaag agtgcgagcc tctaccacta ccgctggttg ctccaaaact tctgccgcag
    19081 attgagctgc catgaagatg ttcttgttgt ttggcaagaa gatgatgtta cgggcattaa
    19141 cctgttcaac agccttgata aagtcttctg ttgaagggtt catggtttga ccgccttcga
    19201 taacataatc cacgccttga gaacagaaga tatctgctag acctttacca gccaccacag
    19261 caatcaaagc atactctttt tcttcagccg acttgataac ttgagtagct tctttctcaa
    19321 cctgtgcttc gtgttggtta cgcatattgt caacttttac cttgaccaag ctaccatatt
    19381 tgagaccttc ttgcataaca agtcctggat cttctgtatg aacatggact ttgacaattt
    19441 catcatcgtt aacaacgagg agagaatctc caagctcatc caagtagtta cggaattcat
    19501 cgtagtcaaa atctttagca taggttggac cttgcttaag agctaccatg atttcagtac
    19561 agtaaccaaa cgtgatgtcc tcagtcgcta cgtgaccagc tacagactta tgatgctcta
    19621 cattgatcat ctcactcatg ttggcaggag tcgctacaaa gtcctcagat gcaatatatt
    19681 cgccagtaag ggctgaaagg aaaccttcgt agatgaagac caatccttga ccacctgagt
    19741 ccacaacgcc aacttctttc aatactggaa gcatgtctgg tgttttagct agagctgttt
    19801 tagcaccttc caaggctgcg cgcatgactt caacagtgtc atctgtttgc tcagcttttt
    19861 tcttagcacc gatagcagct ccacgagaaa ctgtcaaaat cgttccttca acaggtttca
    19921 tcactgcctt ataggcaact tccacacctg attggaaggc cagagccaag tcttgacctg
    19981 ttaactcgtc tttatccttg atagcttggg aaaatccacg gaaaagctga gacgtaatca
    20041 ctcctgagtt cccacgcgca cccatcaaaa gccctttggc aagaatgctc gctacttctc
    20101 caactgtaga agctggcttg tctgcaactt ctttagcacc attttcaatg gtcattccca
    20161 tatttgtccc agtatctcca tctggaactg gaaagacgtt taatgaattg acatattcag
    20221 cttgcttatt caagcgagtt gatgcagcct gcaccatttc ttgaaataag ctagtagtaa
    20281 tttttgacac ggttattctc ctacaacttt gatattttga atgtagacat ttacagtctg
    20341 agcagtaatt ccaagctggt tttccaagct aaaggcaaca cgctcttgaa tgttttttga
    20401 cacttcacta atctttgttc cgtagcttaa cacggtatat acatcaactg caatactgcc
    20461 atcttcggct gcctttacga cgacaccttt agaataattt tccttaccta gcagggcttg
    20521 gaaattatct ttgagggcat ttttactagc cataccgacc acaccaaaaa tctcagttgc
    20581 tgctccacct acgacggttg caatcacttc atctgttagt tcgatttgac catcttttgt
    20641 attaattttt acagtcatcc tttttacctc aactagttga tactctattt tatcatattt
    20701 cagcccaagt gtaaaagcag aatgatgtat cagcggatat ttactctatt tttcaaacga
    20761 tgttataccc acaataaaag aaaaaagacc ctaaggtctc ctttgctttt attattaaac
    20821 gcgttcaact ttacctgatt tcaaagcacg agctgaagcc caaacttttt taggtttacc
    20881 atcgataaga acagtaactt tttgaaggtt tggttttacg gcacgttttg tttggttcat
    20941 cgcgtgtgaa cggttgtttc ctgatacagt cttacgacct gtaaagtaac atactttagc
    21001 cattgtgttt tcctcctatt agatctaata tagcggatgt gctagcacca cataccgtac
    21061 tatgttatca cactttctta ttttttgcaa gggaattgga agatttttta tttgtgtctt
    21121 aaatcaggtc ttgcgtgaca ttttctgctc tccacatgcc attgttgatt aacagaatac
    21181 cagaattaaa attatgtgta taaaaatcat ctctaactgc agctaaggat atagccgtca
    21241 agtccaaatc ccacagctca tctatcgatt ttcttacaac aatatctgaa tccaaataca
    21301 gtacacgaga ctcgcttaca tactttggaa taaaatacct aaaaaagccg catatgaaag
    21361 tccctcaaag gggagacgat aacctttcag aatactactg tcaatctaaa cattcacaat
    21421 ctcactattc aaagtctcta gtcttttttc cattaattgg aaccattctc gcggaaggtc
    21481 atcattaaaa acataaaact taagattata atgatgaaca caaagagatt ttattgttgt
    21541 ttcaacttta tccatataag cattatctgc acctaagaca atcgcttttt tctcttcttt
    21601 cactttttat ctcatttctt ttattcccat catatgtttc tacgtaacca ttattttcgc
    21661 ctattcgttc gtaaaaccat accagtggag attttagatg aagtcccatt acggtttaca
    21721 atttttacat tacgacacgg agtttggcaa atcgattcta tttgccaaac gtagttagtg
    21781 aggcagttag ctagttcgcc aaatagcgac tagcgtccaa caatttggaa ctttagttcc
    21841 aattgttggt actgagtcac atcttctcct ctaactctac gtctggatac ttgtccgcaa
    21901 accagcggag ggcaaagtca ttttcaaaga gaaagactgg ttggtcaaaa cggtctttgg
    21961 ctaagatatt gcgacttgac gacatccgtt catccaagtc ctcaggcttg atccaacgaa
    22021 cggtcttttt acccattggg ttcataacta cttctgcatt gtactcgcct tccatgcggt
    22081 gtttaaagac ttcaaactgg agttgaccta cagcgcctag catgtactca cctgtttggt
    22141 aattcttata aagctgaacg gctccttctt gcaccaattg ctcaatcccc ttgtggaagg
    22201 atttttgctt cataacattc ttagcagaaa ctttcatgaa aatttcagga gtaaaggttg
    22261 gcagtggttc aaattcaaac ttgtttttac caactgtcaa ggtatcccca acttgataag
    22321 taccggtatc gtaaaccccg ataatatcac ctgccacggc attgatcaca ttctcacgac
    22381 tttccgccat aaactgagta acattagaca gtttagcccc cttaccagta cgagggagat
    22441 tgacactcat gccgcgctca aattcgccag atacgatacg gacaaaggca atacggtcac
    22501 ggtgacgagg gtccatgttg gcttggattt taaagacaaa tcctgagaaa tccttgtcat
    22561 aaggatccac aatttcaccg tctgttttct tgtgaccatg tggttctgga gcaaacttga
    22621 ggaaggtttc aaggaaggtc tgcacaccaa agtttgtcag ggctgaaccg aaaaagacag
    22681 gcgtcaattc tccagccaga atagcttcct ctgaaaactc attcccggct tcatttaaaa
    22741 gctcaatatc atccttgact tgctcgtaga aaggattgct accaaaaagt ttgtccccat
    22801 cttctagact ggcaaaacgc tcatcccctt tataaagctc taaacgttgg ttatagaggt
    22861 catacaagcc ctcaaaggct ttccccatcc cgataggcca gttcataggg tagctagcaa
    22921 tgcccaagat ttcttccaat tcttgtaaga gatccaaagg ctcacgaccg tcacggtcca
    22981 gcttgttcat aaaggtaaag actggaatgc cacgatgttt cacaacctca aacaatttct
    23041 tggtttgagc ctcgatcccc ttggcagagt ccacgaccat gaccgcagca tccaccgcca
    23101 tcaaggtacg ataggtatct tctgagaagt cctcgtgccc tggcgtgtct aagatattca
    23161 cgcgcttgcc gtcgtagtca aattgcataa cagatgaagt aacagaaatc ccacgttgct
    23221 tctcgatatc catccagtca gatttagcaa aagtccctgt tttcttccct tttaccgtac
    23281 cagcctcacg aatctcaccc ccaaagtaga gtaactgctc agtgatggtt gttttccccg
    23341 cgtccgggtg ggagataatg gcaaaggtac gacgtttctt aatttcttct tgaatattca
    23401 taagttctct ttctttgatt ctctattttt cttgtttcaa tagctgagaa tgatttttac
    23461 attggatttt accattcctt tcaacactcc attatatcgg attttagcat ttttttcaat
    23521 ttctatttct tttcacttcc ccctccctta tttataggaa aatatggtaa aatagaacag
    23581 actaaaaatc atcatttcac gaaaggatgc aagatgaaaa ttacgcaaga agaggtaaca
    23641 cacgttgcca atctttcaaa attaagattc tctgaaaaag aaactgctgc ctttgcgacc
    23701 accttgtcta agattgttga catggttgaa ttgctgggcg aagttgacac aactggtgtc
    23761 gcacctacta cgactatggc tgaccgcaag actgtactcc gccctgatgt ggccgaagaa
    23821 ggaacagacc gtgatcgctt gtttaaaaac gtacctgaaa aagacaacta ctatatcaag
    23881 gtgccagcta tcctagacga tggaggagat gcctaatgac ttttaacaat aaaactattg
    23941 aagagttgca caatctcctt gtctctaagg aaatttctgc aacagaattg acccaagcaa
    24001 cacttgaaaa tatcaagtct cgtgaggaag ccctcaattc atttgtcacc atcgctgagg
    24061 agcaagctct tgttcaagct aaagccattg atgaagctgg aattgatgct gacaatgtcc
    24121 tttcaggaat tccacttgct gttaaggata acatctctac agacggtatt ctcacaactg
    24181 ctgcctcaaa aatgctctac aactatgagc caatctttga tgcgacagct gttgccaatg
    24241 caaaaaccaa gggcatgatt gtcgttggaa agaccaacat ggacgaattt gctatgggtg
    24301 gttcaggtga aacttcacac tacggagcaa ctaaaaacgc ttgggaccac agcaaggttc
    24361 ctggtgggtc atcaagtggt tctgccgcag ctgtagcctc aggacaagtt cgcttgtcac
    24421 ttggttctga tactggtggt tccatccgcc aacctgctgc cttcaacgga atcgttggtc
    24481 tcaaaccaac ctacggaaca gtttcacgtt tcggtctcat tgcctttggt agctcattag
    24541 accagattgg accttttgct cctactgtta aggaaaatgc cctcttgctc aacgctattg
    24601 ccagcgaaga tgctaaagac tctacttctg ctcctgtccg catcgccgac tttacttcaa
    24661 aaatcggcca agacatcaag ggtatgaaaa tcgctttgcc taaggaatac ctcggtgaag
    24721 gaattaaccc agaggttaag gaaaccattc taaatgccgc taaacacttt gaaaaattgg
    24781 gtgctattgt cgaagaagtc agccttcctc actctaaata cggagttgcc gtatactaca
    24841 tcatcgcttc atcagaagct tcatcaaact tgcaacgctt tgacggtatc cgttacggct
    24901 atcgcgcaga agatgcaacc aaccttgatg aaatctatgt aaacagccga agccaaggtt
    24961 ttggtgaaga agtgaagcgc cgtatcatgc tgggtacttt cagtctttca tcaggttact
    25021 acgatgccta ctataagaag gctggacagg tccgtacact tatcattcaa gatttcgaaa
    25081 aagtcttcgc ggattacgat ttgattttgg gtccaactgc tccaagtgtt gcctatgact
    25141 tggattctct caaccatgac ccagttgcca tgtacttagc cgacctattg accatacctg
    25201 taaacttggc aggactgcct ggaatttcga ttcctgctgg attctctcaa ggtctacctg
    25261 tcggtctgca attgattggt cccaagcact ctgaggaaac catttaccaa gttgctgctg
    25321 cctttgaagc aacaacaggc taccacaaac aacaacccgt gatttttgga ggtgacaact
    25381 aatgaacttt gaaacagtca tcggacttga agtccacgta gagctcaaca ccaattcaaa
    25441 aatcttctca cctacttctg cccacttcgg aaatgatcaa aatgccaaca ctaacgtgat
    25501 tgactggtct ttcccaggag ttctgccagt tctcaataaa ggggttgtcg atgccggaat
    25561 caaggctgct cttgccctca acatggacat ccacaaaaag atgcactttg accgcaagaa
    25621 ctacttctac cctgataatc ccaaagccta ccaaatttct cagtttgatg agccaatcgg
    25681 ttataacggc tggattgaag tcgaactaga agacggtacg accaagaaaa tcggtatcga
    25741 acgtgcccac ctagaggaag acgctggtaa aaacacccat ggtacagatg gctactctta
    25801 tgttgacctc aaccgccaag gggttccctt gattgagatt gtatctgagg cagatatgcg
    25861 ttctcctgaa gaagcctatg cttatctgac agccctcaag gaagttatcc agtacgctgg
    25921 catttctgac gttaagatgg aggaaggttc gatgcgtgtg gatgccaaca tctcccttcg
    25981 tccttatggt caagagaaat tcggtaccaa gactgaattg aagaacctca actccttctc
    26041 aaacgttcgt aaaggtcttg aatacgaagt ccaacgccag gctgaaattc ttcgctcagg
    26101 tggtcaaatc cgccaagaaa cacgccgtta cgatgaagcg aataaagcaa ccatcctcat
    26161 gcgtgtcaag gaaggggctg ctgactaccg ctacttccca gaaccagacc tacccctctt
    26221 tgaaatttct gacgagtgga ttgaggaaat gcggactgag ttgccagagt ttccaaaaga
    26281 acgtcgtgcg cgttatgtat ctgaccttgg tttatcagac tacgatgcta gtcagttgac
    26341 tgctaataaa gtcacttctg atttctttga aaaagctgtt gccctaggtg gtgatgccaa
    26401 acaagtctct aactggctcc aaggggaagt cgctcagttc ttgaatgctg aaggtaaaac
    26461 actggaacaa atcgaattga caccagaaaa cttggttgaa atgattacca tcatcgaaga
    26521 cggtactatt tcatctaaga ttgccaagaa agtctttgtc catctagcta aaaatggcgg
    26581 tggcgcgcgt gaatacgtgg aaaaagctgg tatggttcaa atttcagatc cagctatctt
    26641 gatcccaatc atccaccaag tctttgccga taacgaagct gctgttgccg acttcaagtc
    26701 aggcaaacgt aacgccgaca aggcctttac aggattcctt atgaaggcaa ccaaaggcca
    26761 agccaaccca caagttgccc ttaaactact tgcacaggaa ttggcgaagt tgaaagaaaa
    26821 ctagacagaa caaaaccagc cctaaggttg gttttttctt ctctaccaac tcccaataac
    26881 tattttggct ttatttccag agtattttat ggtaaaatga agagtaataa tatttattaa
    26941 agaggtaaaa acatgattga agcaagtaaa ttaaaagctg gtatgacctt tgaaacagct
    27001 gacggcaaat tgattcgcgt tttggaagct agtcaccaca aaccaggtaa aggaaacacg
    27061 atcatgcgta tgaaattgcg tgatgtccgt actggttcta catttgacac aagctaccgt
    27121 ccagaggaaa aatttgaaca agctattatc gagactgtcc cagctcaata cttgtacaaa
    27181 atggatgaca cagcatactt catgaataca gaaacttacg accaatacga aatccctgta
    27241 gtcaatgttg aaaacgaatt gctttacatc cttgaaaact ctgatgtgaa aatccaattc
    27301 tacggaactg aagtgatcgg tgtcaccgtt cctactactg ttgagttgac agttgctgaa
    27361 actcaaccat ctatcaaagg tgctactgtt acaggttctg gtaaaccagc aacgatggaa
    27421 actggacttg tcgtaaacgt tccagacttc atcgaagcag gacaaaaact cgttatcaac
    27481 actgcagaag gaacttacgt ttctcgtgcc taatctctag aaagaggtca ttctatggga
    27541 attgaagaac aacttggcga aatcgttatc gccccacgtg tacttgaaaa aatcattgct
    27601 atcgctactg caaaggtaga gggggttcac tctttttcaa acagatcagt gtctgatacc
    27661 ctttcaaaac tttcactcgg ccgtggcatt tatcttaaaa acgtggacga agaactcaca
    27721 gcagatatct atctctacct tgagtacgga gtaaaagttc ctaaggtagc ggttgctatc
    27781 cagaaagctg tcaaagatgc cgtccgtaat atggctgatg tagaactcgc tgctatcaat
    27841 attcacgttg caggtatcgt cccagataaa acaccaaaac cagaattgaa agatctattt
    27901 gacgaggact tcctcaatga ctagtccact attagaatct agacgccaac tccgtaaatg
    27961 cgcttttcaa gctctcatga gccttgagtt cggtacggat gtcgaaactg cttgtcgttt
    28021 cgcctatact catgatcgtg aagatacgga tgtacaactt ccagcctttt tgatagacct
    28081 cgtttctggt gttcaagcta aaaaggaaga actagataag caaatcactc agcatttaaa
    28141 agcaggttgg accattgaac gcttaacgct cgtggagaga aacctccttc gcttgggagt
    28201 ctttgaaatc acttcatttg acactcctca gctggttgct gttaatgaag ctatcgagct
    28261 tgcaaaggac ttctccgatc aaaaatctgc ccgttttatc aatggactgc tcagccagtt
    28321 tgtaacagaa gaacaataaa aagaaaaagt gtttgatatc tatcaaacac tttttctggc
    28381 tcccactatc taaaaaaatg ataatagata taattgtaaa caaaaatcca gataggtttt
    28441 gcatgattga gaaagttaaa aaaactatgg cagagaatcg ttaatctcag attgtcggta
    28501 gaacgataaa caagggcaaa aaagaaacca atcagactat aatataataa actaattgga
    28561 tctctgtgag atagtatcaa atggctaatc ccaaagatga tagcagatag gataacatcc
    28621 aaatagtact tggactaggg aaagaaggta ttcataaaat accctctatc aagagtctcc
    28681 tcaaaaacag gaccgatgat tacaggcagg acaaaagata agatagtcga taaaaaggtt
    28741 ggttgtccat ttgaaaaaag cacggtaaaa tactcatcat gaatattcct atgattaatc
    28801 aaatgagcat agcgtgccca aaaattaccg agaatctgat aaaccacata agttgcaaat
    28861 aagtagaaga caaatgacca gttccagctc tttttctcaa agataaagag catctttttc
    28921 ttttttaacc tccaaattaa tagaaggaaa cttcccacta atcccattgt taaaataaga
    28981 gaatagacat cagctcctaa ccctaaaatg atcgtcacat acaatccaat tgtttgtggt
    29041 aaataggtag atagtaaaat aataagcaaa aatattccaa attgtcttag tttttttgtg
    29101 tttctcatcg tacttttttg aaagattacc ctgctcggaa gccgtacttc caagcatcta
    29161 tataagaatt aagtgcccct tgcctcatat agggagcaaa ttctctataa tataaccatc
    29221 tactatatcc atcttcccaa acagcaagac cacctgaagt ttgctccaag tcctcagttg
    29281 aaagaactgt aaatgtattt gtacctgtca ttgcaagtac cttcttaaaa tagattgttg
    29341 taggctcaca tttatagtat atttcttttt ttgtctattt tatagcccat ctcctcaact
    29401 ggcaattttt cgacctgaat tacatttttc cataaaaaat gagacctttc tagtctcatt
    29461 tagtcattct tagtattttc taaatcgttg atagcgttct tccagcaact cttctagcgg
    29521 tttttgtgaa agtctagcca gctccgtttg gagttctttt ttgacactct taatcagttc
    29581 tttactagaa agtcctattt cagaaatcac cttatccacc acgtccattt ctaacagttc
    29641 atgcgaagtg attttcatca gttctgctgc ttccatagcg cgagtaccgt ccttccataa
    29701 aatggaagca aagccttctg gactgagaat ggcatagata gaattttcca gcatccagac
    29761 acggtccgcg acagctagag ccagagcccc gcctgaacca ccttcaccga taataatggc
    29821 gataatagga actttcaggt cactcatttc catgagattg cgagcgatag cttccccttg
    29881 accacgttct tccgctccga caccaggata agcacctgct gtattgataa aggtcacaac
    29941 tggacggcca aatttctcag cctgtttcat caaccgcagt gcctttcggt agccttctgg
    30001 atgtggttgg ccaaaattcc gtttgaggtt gtcttgcaaa ctcttgcctt tttggatacc
    30061 aaccactgtt acagcttgat ctccaagcca accaatacca ccaacaactg caccatcatc
    30121 acgaaaagaa cggtcaccat gtaattggat aaattcatca aaaatgcctg tcgcaaaatc
    30181 caaggttgtc aagcgactct gctcacgcgc ttctctgact atttttgcaa tattcatcta
    30241 ggactccctc catgcaatct gactaggcta gcaatcgtat ctggtaagtc tcttcttttg
    30301 acaatagcat ccacaaagcc atgttctaat aggaattctg ccttttggaa atcctcaggc
    30361 aagctttcac gaaccgtatt ttcaatcaca cgacgcccag caaaaccaac caagctctgt
    30421 ggttcagcca gaatgatatc gccttccata gcgaaagaag ctgtcacacc accagtcgtt
    30481 ggatctgtca aaatggtcag gtaaaagaga ccagcatttg aatgacgttt aaccgccgca
    30541 gagatcttag ccatctgcat gagactcatg attccttcct gcatacgggc tccaccagag
    30601 gctgtgaata ggacaactgg caatttttcg acagtcgcat actcaaacaa acgagtgatt
    30661 ttttcaccta caaccgtacc catagaagcc atgataaagt tagaatccat aatcccaaga
    30721 gccacagtct gacctttaat aagagcagtt cctgtcacaa cggcttcatg cagacctgtt
    30781 ttttcacgca tagatgccag tttcttttgg taaccaggga aatgcaaggg atccttgctt
    30841 tcaatccctg taaacaattc tttgaaggtt cccatatcaa tcgtcaaagc caagcgttct
    30901 tgggcagaaa tacgaaaggt atagctacag tgcggacaga tacgttcact tcccagatcc
    30961 ttctgataga tggtatgctt acagcctgga cactgggaaa ataattcatc tggaacctct
    31021 ggcttagctt gaggtttttc cctaactgaa cgattgggat tgattcgaat atacttatct
    31081 tttttactaa atagagccat tgattcccct tttcggttta aactcttaaa gtcattttat
    31141 tctttttctt gatatttagg taagaaggtt tccatcaaga aggaagtatc ataatcccca
    31201 gcaatgacat tgcgatctga aatgaggtca agctggaaat ctgcattggt ctgcactcct
    31261 tcaatctcta attcatagag ggcacgttgc attttcatca aggcgtcaaa acgattttcg
    31321 ccgtgtacta tgattttggc aatcatacta tcataataag gcggaatggt ataacctgga
    31381 taaactgctg aatccacgcg caagccaact ccaccacttg gcagatagag attagtaatc
    31441 ttacctggac ttggagcaaa gttaaaggct gggttttctg cattgatacg acactcgatg
    31501 gcatgaccgc gtaggacaat atcttcttgc ttaacagaca aaggctgacc tgccgcaatg
    31561 caaatctgtt ccttaacgat atcaacacct gaaacaaact ctgttactgg atgttctacc
    31621 tgaacacgag tattcatctc catgaaatag aaattgctac ttgcttcatc aagaagaaat
    31681 tcaatggttc ctgcattctc atagccaaca aactctgccg ctcgaacagc agcagcacct
    31741 atttcatgac gcagcgtttt tccgattgca atcgagggac tttcttccaa aaccttttgg
    31801 ttattccttt gaagagaaca atcccgttca cccaagtgaa tcacatgtcc atgctcatca
    31861 cctaggattt gaacctcaat gtgccgagct ggatagataa cccgttctat gtacatggca
    31921 ccattgccat aattggcctt ggcctcacta gaggcagttt caaaggcaga aacgaggtca
    31981 tctggttttt caaccttacg aatcccttta ccacctccac ctgctgaagc cttgagcata
    32041 acaggatagc caattttttc agcaacaatc aaagcttctt cagagttatg cacttctcca
    32101 tctgaacctg gtataacagg cacacctgct ttaatcatct gagcacgcgc attgatctta
    32161 tcccccatca tatccataac ataaccagat ggaccgataa acttgatacc tacttcttca
    32221 cacatggtcg caaatttgga attttcactg agaaatccaa aaccagggtg aatagcttct
    32281 gcctcagtca agactgcagc tgatagaact gcattaatat tgagataaga ctctgttgcc
    32341 ttgccaggac caatacaaac tgcttcatct gccaaaagcg tatgaagagc ttccttatca
    32401 gcagttgaat aaaccgctac cgtcgcaatc cccaattcac gtgccgcacg gataatacga
    32461 accgcaattt caccacgatt ggcaattaaa atttttcgaa acatggagaa cctccttagt
    32521 tcccaattgc aaaagtaagg ataccactgg ctgcaagctt gccatccact tcagcctttg
    32581 cttcaaccac agctatggtg ccacgacgtt ttacaaaagt cgctgtcata accaattggt
    32641 cgcctggtac aacttgcttc ttgaacttaa ccttgtccat accagcgtaa aagaccagtt
    32701 ttcctttatt ttcaggtttt gataactcca acacaccagc agtttgcgcc aaggcttcca
    32761 taatcagaac acctggcata actgggtatt gaggaaagtg gccgttaaag aaaggctcgt
    32821 tgatggtcac atttttgata gcaacaatgg tattatcgct cacttccaag acacggtcca
    32881 ctagaagcat aggataacgg tggggaaggg cttctttgat tccttgaata tcgatcattt
    32941 gatacgtacc aatcctttgc caaactcaac catttcctcg ttagagacga gaatttccgt
    33001 taccacacca tccttaggag ctgggatttc attcatgact ttcatggctt cgataattac
    33061 caatgtttga ccttttttga cactatcacc aactgtaacg aaggcaggtt tatctggtcc
    33121 agcagccaag taaaccactc caacaagtgg actctctaca agatttccct cagtagccac
    33181 acttgcttct gctggagctg gaacttcttc tgctacagtc tctgctggag cagatgtagg
    33241 agctactgga ctcggtgttg ctagaacggg tgctggagcg acttgagttg caacttcagg
    33301 cacaggtctc gcttcattct tgctaaactg caactcatcc gtcccatttt tataagaaaa
    33361 ttctctcaaa cttgactggt caaattgagt catcaagtct ttaatatcgt ttaaattcat
    33421 acttatctat tctcccaacg tttgaaagca agaactgcat tgtggcctcc aaaaccaaaa
    33481 gtatttgaaa tagcgtatgg aatttctttc tccaagcctt gtccataaac gacattaact
    33541 tcgatataat ctgatacttc acttgtccca gctgtcattg gtacaaagtt atgacgcata
    33601 gcttcgatgg tgacgatagc ttctactgca cccgcagccc ccagcaaatg tcctgtaaaa
    33661 gacttggttg atgatacagg tacttcctta ccaagaacag ctacgatagc tccactttct
    33721 cctttttcat tggcaggagt tgacgttccg tgagcattga cataggctac ttgctctgga
    33781 gaaatctcag cttcttccaa ggctagtttg atggccttga tagctccctg accttctgga
    33841 tgtggagaag tcatgtggta ggcatcacaa gtatttccgt aaccaaccac ttcagccagg
    33901 atagtagctc cacgtttttc agcgtgttca agactttcta gaaccaacat ccctgaacct
    33961 tcacccataa caaacccatt gcgatcctta tcaaatggga tcgaagcacg agttggatcc
    34021 tctgtagtag agagagctgt taaggcttgg aaaccagcga tggcaaaagg tgtgatagaa
    34081 gcttctgttc ctcccaccaa catcacatct tggaaaccaa acttaatgga gcggaaggca
    34141 tccccaatcg catcatttga tgaagagcag gcagtattga tagatttaca aacaccgttt
    34201 gcaccaaaac gcatggctac attcccagaa gccatatttg gtaaagcttt tggaagagtc
    34261 attggtttga cacgtttggg tcctttttca tgaaggcgaa gtacctgatc ttcaatttcc
    34321 ttgattccac caataccaga tgcaacgata acaccaaaac gatccctatt aagagcctct
    34381 acatcaagat tggcatgatt tacagcctct tgggctgcat acaaggcata taaagaatag
    34441 ttatcaaaac ggttggtatc tttttttaca aagtatttat cgaacggaaa atcttggatt
    34501 tctgccgcat tatgcacatc aaagtcacta tgatcaaatt ttgtaatgcc accaatgccg
    34561 attttcccag ttgctaaact attccaaaat tcttctggtg tatttccgat tggagatgtt
    34621 actccataac ctgttaccac tactcgattt agtttcattc ttttcacctc tagctttcgc
    34681 tacatactta agccaccatc aatggcaacc acttgaccag ttagataatc ttggcctgct
    34741 aaaaatactg tcaaatctgc aacctgctct gcctgcccaa attctttcat cggaatctga
    34801 gctagtgtag cttccttaat cttatctgat aagatagctg tcatatcaga ctcaatcatt
    34861 cctggagcaa tcacattgac tcgtatattc cgactagcga cctcgcgtgc cacagacttg
    34921 gtaaagccaa tcaagccagc cttagaagca gcatagttag cttgaccaat attccccatc
    34981 aaaccaacaa cactagacat attaatgata gcaccttctc tggctttcat catcggtttc
    35041 aagactgatt gtgtcatatt aaaggcacca gtcagattga ccttgagcac tttttcaaaa
    35101 tctgcttctg tcatcttgag cataagagta tcttgggtaa tccctgcatt gttgaccaaa
    35161 acatctactg aacccagttc tgcaatagct tgatcaatca tacgcttagc gtctgcaaaa
    35221 tctgatacat ctcctgaaat gggaaccacc ttgataccat agtttgaaaa ctcagcgagc
    35281 aattcttctg agattgcccc acgactgttt aagacaatgt tggctcctgc ttgagcaaac
    35341 ttgtgggcga tggcaagacc aattccacga ctcgaacctg taataaagat atttttatgt
    35401 tctagtttca tttttttcct ttcaaaactt ctacttattt tagtctattt ttctaaaagt
    35461 gctactaaac tcgcttgatc ttccacatga gctaagtgag cagtttgatc aattttttta
    35521 acaaaacctg acaagacttt ccccggtcca atctcgataa agttgcttat gcctgcttct
    35581 tgcatgaccc caatactttc atagaaacga acgggttcct tgacctgacg cgtcaagagc
    35641 tgagcaatgt cctctttttg catcacagca gcttctgtat tgccgactag gggacaagta
    35701 aaatttgaaa aacttacctg agctagagtt tcagctagtt tctggctagc aggctcaagg
    35761 agagcggtgt gaaagggacc tgacacctta agaggaatca agcgtttggc acctgcttct
    35821 tgcaaaagtt caaccgctcg atcaactgca accacttctc cagcaatgac gatttgtgca
    35881 ggtgtgttat agttggctgg agtaaccact ccaagttcag aagctttttg acaggcttct
    35941 tcaatgacct ctactggcgt attgagaact gctaccatct tgccagagtc agcaggagcc
    36001 gcttcttcca tataggctcc acgcttagct accaaggcaa ccgcatcttc aaaatccaag
    36061 gcgccgcttg ccaccaaggc agagtattct ccaagagaca aaccagcaac catatcaggc
    36121 tgatagccct tttcttgcaa taaacggtag atagcaaccg aagtcgctag aatggctggt
    36181 tgcgtatagc gggtctgatt gagtttgtct tcttccgtat cgatgagata acgtaaatca
    36241 taaccgagca cctgactcgc tcgatcaatc gtttctttga caatcggata ctgatcatag
    36301 aaatcccgtc ccatccctag atactgggca ccttgaccag caaataaaaa ggctgtttta
    36361 gtcatttctt acaactcctg cccagcgaga ggcttcttct tgaattttct tagcggctcc
    36421 gtaatacaaa tcttttagga tttcttcagc tgtttcttct ttagaaacaa gccctgcgat
    36481 ttgacctgcc atgacagagc caccatccac atcaccgtga acaactgctt tggctagggc
    36541 acctgctccc atttgttcaa agatttctaa atcaggatct tcctgcttaa aggcatcttt
    36601 ttcagccagt tcaaaatctc tagtcaactg atttttaata gcacgaacag catgaccaaa
    36661 gtgctgagct gaaatcgtag tatcaatatc ccttgctttt aaaattttct ccttgtagtt
    36721 tggatgggca ttcgactctt ttgcaactac aaaccgtgtc cccacctgta cagcctctgc
    36781 acctagcata aagccagccg cagcaccttc accatccgca attcctcctg cagcaataac
    36841 aggaatagat atagctgtgg ctacctgtcg caccaaggtc atggttgtta atttaccgat
    36901 atgcccccca gcttccattc cttctgcaat aacagcgtct gcaccgattt tttccatgcg
    36961 tttagctaaa gcgacactag gaacaacagg aataacgatt atcccagctt catggaaacg
    37021 ttccatatac ttgcttggat ttcctgctcc tgttgtgaca actttaacac cttcttcaat
    37081 aacgagatcc acgatatctt ccacaaaggg agataagagc atgatgttga ccccaaaggg
    37141 tttatcagtc aatgatttga ttttatcaat attggccttg acaacttctt tcggggcatt
    37201 tcccccaccg ataattccta atcctccagc cttggaaaca gcccctgcca aatcaccatc
    37261 agcaacccag gccatccctc cttggaaaat aggatagtca atcttcaata attctgtaat
    37321 acgcgttttc atagtgcctc caaccttcct tgcttacgta atagttcgat ttcaccataa
    37381 tttgacagtc aaactattac ctaaacaaga gggagtgggt ttctccctac tccttctact
    37441 aatattctgc ttattttgct tgctcttcaa cgtaagcaac caagtcacca actgttttca
    37501 agtcattttc tgcttcgatt tggatatcaa aagcatcttc gatttctgag attacttgga
    37561 acaagtccaa tgaatctgcg tccaaatcat caaaagttga ttcaagtgtt acttctgatg
    37621 cgtcttttcc aagttcttca acgataattt cttgtacttt ttcaaatact gccatgatag
    37681 gactccttta aaataaatag tttttttata acaatgtgtt caccacatga ttacctaaat
    37741 tgtaagaatg agcgtgcccc aggtcaagcc tccaccgaag cctgatagaa gaacagtctg
    37801 gctaccatct aaagggatga gaccttgttc tacacactct gaaagtaaaa tcgggatact
    37861 ggctgcactg gtattgccat attccatcat attggctgga agtttggctc ggtcaacacc
    37921 aatttttcta gccatcttat ccaaaatacg gtcattggct tgatgaagta gcagataatc
    37981 caagtctgtc acctctatag gagattcatc aatagtctgc ttgatagact tggctacatc
    38041 tcgaatggca aaatcaaaga ctgtgcgtcc atccatcttc aaaaacgaat ctgcactttc
    38101 ttgatctgaa aatggagaat gcaaacctga atgcccataa gttaaacact cgctgcgact
    38161 tccatcgcta ttgagactct cagctaagaa atgctcttgc tcgctagctt ctaacaagac
    38221 accaccagca ccatctccaa acaacacagc tgttgatcga tccgaccaat cgactgcctt
    38281 agagagggtt tcactaccaa tcaccaagcc tttttgaaag cgaccagaag cgataaactt
    38341 ttcagcagtt gaaagagcaa atacaaatcc actgcaagcc gcggttaagt caaaagcaaa
    38401 ggctttatta gcgccaatat tagcttgaac acgagcagct gtagagggca tcatcgaatc
    38461 tggagtaatg gtagctagga tgataaaatc cagttctttt cctgttattc cagcttttgc
    38521 catcagtttc ttagcaacct ctgtagccaa atcactggta gattctgttc ttgaaatatg
    38581 cctttgtcgt attcccgttc gacttgaaat ccactcatca ttggtatcca taatctgagc
    38641 caagtcgtga tttgtaacca cttgctctgg cacataatga gcaacctgac ttatttttgc
    38701 aaaagccatt atttcaaatc ctccaaaaat tggtaaagat tagtcaagcc tttacccatg
    38761 acagcaattt cttcctcgct catgccatca ataatttttt ctaccatggc cttgtggaag
    38821 cgtttatgca gtctatgaat caagcgaccc ttctttgtca aatgcagatg caccacacga
    38881 cgatcctgtt ctgaccgaac tcgctcaatg tagcccttac gctctaaatt gttcaaactt
    38941 gtcgtaacag ttccaagagt taccatcaac tcttttgaca cttgacttgg agtcacatct
    39001 ggagccttac cgatgacatc aatcgtatgc atttctttga tggagatatc cttaaaacga
    39061 ctacctctca agttcacttc ctcaattaca aggacattgt taaatataga tgttaaatat
    39121 tcattgattc gttggtagtc catttttcat atccctcctt cttcaaaaaa cttttacaat
    39181 caaaacattt gacagaaaga attatatcaa aattcaaaga atgatgcaag tattttttat
    39241 tttcctgtaa atttaggtct tcttctctcc gaatgagctc gcactccttc tttgaaatcc
    39301 tctgtttgag ctaaggattt ctgtaggttc agttctaaag tagcgtaacc ctgccagtct
    39361 ttaaattggc tctcccaaac caacttctta atggcagcat aggaattact tgaagcacgt
    39421 cttaattttt taagaagctg ttctctcgtc ttttcaagtt tttcagcttc agaaacgcgg
    39481 taaaccaacc cccattctag ggctttttct gccgttaaag cctctcctgt catagctaat
    39541 tgagcagcac gcgtcacacc gatactgcgg ctcaagagat gaattccccc tgcatctgga
    39601 gccaaaccaa caccaacaaa ggcttggata aacttagcct tatccgtcgc caaacagaaa
    39661 tctgccgcaa cagccatatt cgctgcggca cctgcaacag caccgtcaac ttccattaaa
    39721 acaggtttgg ctatttgctt gattttataa gaaatcgtat tgaccaactc tgcgattttt
    39781 gtcaatgatg gaatatcatc ctcatccact gcccgcttca tctctaccaa atctccccca
    39841 actgagaaga cctttccatt ggcattgatt aagataaaat gcacagctgg attttcttct
    39901 gccaaagtca gagcttctaa aatctcctca cacatgggaa tatgaaaacc atttgcgacc
    39961 tcaggacggt tcaaggtaag gattgccaag tcctcttcaa gctgataaat aatgtgttcc
    40021 atcagggctc ctttttattt tataagcagt ttatactctt cgaaaatctc ttcaaaccgc
    40081 gtcagcttcc atctgcaacc tcaaaacagt attttgagct gacttcgtca gtcttattta
    40141 caacctcaaa gcagtgcttt gagcagtctg cggctagctt cctagtttgc tctttgattt
    40201 tcattgagtg ttaaattatt tcactgtcaa agtatatctt ttttaggagc agaaagcaag
    40261 aaaaaatcga ctgaaagtgt ctcagccgat aagaaaggct cgctttaggc tagaacgcat
    40321 cttattttct tcattgaaaa aagcctttct ttctgctata atcgtataaa atacttactt
    40381 taggagttct tatgaaagtt gttaaatttg gaggtagttc tcttgcctct gctagtcaat
    40441 tagaaaaagt tttaaacatc gtcaaaagcg attcagagcg tcgttttgta gtcgtttctg
    40501 cgcctggtaa acgcaatgct gaagatacta aggttacgga tgccctgatt aaatactacc
    40561 gcgactatgt tgcgggtaac gatattagca agagccaaag ctggattatc gaccgctatg
    40621 ctgctatggt tagtgaattg ggactaaaac cagctgtgct agaaaaaatt tctaaaagca
    40681 ttcacgcctt ggccactctt cctattgaag aaaatgaatt tctctacgat actttcctag
    40741 cagccggtga aaataacaat gccaaattga ttgctgccta ctttaaccaa aatggtatcg
    40801 atgcacgcta tatgcaccct agagaagctg ggattgtggt cacaagtgaa cctggtcacg
    40861 ctcgcatcat tccatcaagt tatgacaaga ttgaagaatt gacaaacacc aatgaagtcc
    40921 ttgtcattcc tggtttcttt ggtgtcacta aggaaaatca aatctgtact ttctcacgtg
    40981 gaggttctga tattacaggt tctatcattg ctgctggtgt caaagccgac ctctatgaaa
    41041 actttacgga cgttgatggt atctttgcag cccaccctgg tattatccac caaccacact
    41101 cgattcctga gttgacctac cgtgaaatgc gcgagttggc ctatgcaggc ttctcagtcc
    41161 ttcatgacga ggctcttctt cctgcctacc gtggaaaaat tcctctggtt atcaagaata
    41221 ccaacaaccc tgaccatcca ggtactcgta tcgttctaaa acacagtaat gatgaatttc
    41281 cagttgtggg aattgctggt gactcaggct ttgtcagtat taacatgtcg aaatacctca
    41341 tgaaccgtga ggttggattt ggccgcaagg ttctgcaaat cctggaagaa cttaacatcg
    41401 gttgggaaca tatgccaaca ggtatcgacg atctttctat cattctccgt tctcgccaac
    41461 taactcccat caaggaagaa gaaatcctgc gtcagttggt tcaaaaggct aaagtagatc
    41521 atgcagaaat cgaacacgac ctttctatca ttatgattgt tggtgaaaaa atgaagagcc
    41581 acatcggagt gactgctact gcgacacgcg ctctatctga aaacaaaatc aacatccaga
    41641 tgatgtctca aggttctagt gaagtttcta ttatgtttgt tgtcaataaa gaccaagaga
    41701 aagcagcaat taaagccctc tacaatgcct tttttggaga aagtaaggaa gactaagcat
    41761 ggctcaatca cttaacaaaa cagtactcct cagtacgaca ggcacttcct acctctctat
    41821 cgctgggaaa gttgggaaat tccttgtcgg agatcaggct ttggaatttt acccagatgt
    41881 caatgtcgaa caatttatcc agattccttg gagccatatc aatcagattg gagccaatgt
    41941 cactggatgc aaaatcagcc gccacttcga agtctttaca gacaaaggaa aattcctctt
    42001 tgcttcaaaa gactcaggtg ccattctcaa aattgcacgc gaaaaactag gcaatgacaa
    42061 ggtcgtcaaa cttccgaccc tgattcagac aattagccaa aaatttaaaa atctatttgc
    42121 aaaaaagtag aaactttagt ataatcatag caacggataa gtaggttatc ttcttctttc
    42181 agaaagtctg cgggtactgc gagcagatag gagggatagg tgaaattcta ccgttagtaa
    42241 caattacata cacaatcaag tgcacacctg tgaagttgga tggaaccgtg gccctgccac
    42301 tccaacgttt tgtcaggcgt gcttttttca taaaggagtt cttatgttag atatcaaacg
    42361 tattcgtaca gattttgaag ctgtcgcaga aaaattagct acacgtggtg tagatgctgc
    42421 tgtcttgaat gaaatgaaag aaatcgatgc taaacgtcgt aacatcttgg tcaaggttga
    42481 aactctcaaa gcagaacgta acacagtttc tgctgagatt gcccaagcta agcgcaacaa
    42541 ggaaaataca gatgacaaga ttgctgccat gcaaaatcta tctgctgagg ttaaagcctt
    42601 ggatgctgaa ttggcagaaa tcgatgctaa attgacagaa tttacaacga ctcttccaaa
    42661 tatcccagct gacagcgttc ctgttggggc tgacgaagac gacaatgtgg aagttcgccg
    42721 ttggggtact ccacgcgagt ttgacttcga acctaaagct cactgggatc tcggtgaaga
    42781 ccttggtatc cttgactggg aacgcggtgg taaggtaaca ggcgctcgct tcctctttta
    42841 taaaggcctc ggtgctcgtt tggaacgtgc tatctacaac tttatgttgg atgaacatgg
    42901 aaaagaaggc tatactgaag tcatcacacc ttacatagtc aaccatgatt ctatgtttgg
    42961 tactggtcag tatccaaaat ttaaggaaga tacttttgaa ctcagcgata ccaactttgt
    43021 cttgattcca actgctgaag ttcctctgac aaactactac cgtgatgaaa tcttagacgg
    43081 caaagatctt ccaatctact tcactgctat gagtccgtca ttccgttctg aggctggttc
    43141 tgccggtcgt gatacgcgtg gcttgatccg tttgcaccaa ttccacaagg ttgaaatggt
    43201 caaatttgcc aaaccagaag aatcttacga agaattggaa aaaatgacag ccaacgctga
    43261 aaacattctt caaaaactca accttccata ccgtgtcgtt gctctctcta ctggagatat
    43321 gggcttctca gctacgaaga cttacgactt ggaagtgtgg attccagcac aaaacaatta
    43381 ccgtgaaatc tcaagctgtt caaacacaga agatttccaa gcccgtcgtg cccaaatccg
    43441 ttaccgtgat gaagcagatg gcaaggtgaa actccttcat accttgaacg gttctggact
    43501 tgcagttgga cgtacagtgg ctgcaattct tgaaaattac caaaatgaag atggttctgt
    43561 gaccatccca gaagcacttc gtccatacat gggtggagct gaagtcatca aaccataaaa
    43621 aataaggttt agctatttct agctagacct tttttcgtaa ccaaatcaga taagcaccta
    43681 gtacaaagaa taaaatagtt aggcatataa tggtttcagc caataccagg taatccagaa
    43741 atggaagttt caaaattccc tgagccatct tgagcgaggt cgctgtgata atggttggga
    43801 aggtgagggc tgagaaggct ggttgaaaac cttgttttaa aatgttgggc agacgagtta
    43861 aaacaaagaa aaagaaggat tgagaagcca aaatcatgac aatcaagacc caagtcggca
    43921 ggctggttcc tcctactcga actagagaag ccaagagtag agagaaagga gcacagtaga
    43981 ttccttcttg tccaagcaag gctagtggga gtggatgttt ctttaaatcg ctataaataa
    44041 ggggatagag atagaaggtc aagagaaaac caaaactcaa ggtcgcatag gcaatttcga
    44101 taatacctac cagaggatag gtcaaggcag ccactgctat ccccacatag agaaccgtcc
    44161 agcttggagt ggcatgaacc ctccgctctg gacaagcaaa cttgatggta aaaccagcaa
    44221 tcaaggtcaa atccaagaga aatgaaaacc accaaatccc ttgtgctacc aaaggaagat
    44281 aagagaatac gcgaaagaca taggtcgata aaatcatccc agccatagga aaggttgcca
    44341 ttcctgacaa aagagggggc ttggtcaatt cttgcttggt ttctttccaa ttaaagagat
    44401 gcagaattag aaagtaaatc cataaaacca aaccagtcag actaaaaaga tgggatagaa
    44461 ctggcaacat atctaaaata agatttccag ctcctgccaa acctagcaaa caacctgaaa
    44521 atactaaggg gagttttttc atcctaacct ccaataatca tgttagtttc agtataacat
    44581 aaaagcgctt aaatgaggat ttaaaaaaac gaagtccgat tatttcagac ttcattttac
    44641 tcagatatga attaggcata aggttgcaat tctggattaa ttggtgtatt agctaagttg
    44701 ttggcatagt tacagaggat tgctaggctg acaccgaaaa ccacatccaa ggcattttgt
    44761 tgagtgtagc cagcttctaa aaactcagac aaggcttcat ctcctacacg acccttggta
    44821 ttgataactg ccaaggtaaa cttagctagg gtatccaatt taggatctgt ttcaattgga
    44881 gtacgattgc gaagagcttg aatcaagtca tcattcatct ggatttgttt gatggaaaag
    44941 gctgtgtgac ctgcgacaca gaaggcacaa ccattggtca cggctgccgt gatttgcacc
    45001 acttcacgct caacgggtgt caggctgttg cgacggtgga tagatgagac aatttggtag
    45061 gcttctaaaa cagtcggggc attggccaag agaccgatta ggttgggaat atagccattg
    45121 ttgtcttttt ctactgtttc aagaatttct ttcacttctg ctggtgctga ctctactgta
    45181 tggatagtaa atgttgtcat aagatacctc ttttcttatt attgacacta atattattgg
    45241 aaaatcttat aaaatcctga ttcctaagtt tatctaagat aaagctttat tctctcataa
    45301 gattttcgtt gttatattag tttatcacac ttccaatcac ttgcataata tatattatat
    45361 atcaggctga taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta
    45421 tttgtcaaaa tactttttag tttcagcaat aacgactggc gacaagacca agagggcaat
    45481 caagtttggc agagccatca aggcgttaac gatatctgcg ataatccaga ccatatccaa
    45541 ctcgataaat cctcctaaca agaccatgag cacaaaaacc acacggtaga gccagataaa
    45601 gcgagcccca aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt
    45661 tgtaaaggca aaaagtacaa ggaagatggt caagagggca ggcccaaagt atgaaaagac
    45721 tgttgagaaa gctgactgag tcaaggcaac cccattcaag tcaccactcc aaactccagt
    45781 taccaagatg gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt
    45841 catggaaatc aaaccttgct ctactggttc atttgtcttg gcagctgcag ctgcaatagg
    45901 agcagaaccc agaccagatt cgtttgagaa cacaccacgc gccacaccat tttgaatagc
    45961 catccgaacg ctagcaccag caaatccacc taccgcagca aggggactaa aagctgaggt
    46021 aaagactaaa gcgattgtgc cagggatttt tccgatatta aagaaaataa ctgtaagagt
    46081 tcctaagata taaatgaggg ccataaaagg aacaacagta gttgaaacct tagaaataga
    46141 cttgagtcca ccaaagactg caatcgctac aaagacagac aagacgagag ctgtgatggc
    46201 tggcgaaatc gtcgttgtat tttggataga ttctgtaatc gagttgactt gggtgaaggt
    46261 tccgattccc aagagagcaa ccaatactcc tgctactgca aacaaaacag caagtggtcg
    46321 ccacttttct cccatcccta gaaggatata gtgcatggga cctcccgcta ctgcaccatg
    46381 gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc gcatacttgg tagccattcc
    46441 aaagaaagcc gccatccaca tccaaaatag agctcctggt ccaccaacct tgatagccgt
    46501 cgcaactcct atgatatttc ctgttccaac agttgatgcc aaggctgtac acagagctgc
    46561 aaaactggat acatcaccat gtcccttatc ctggataaaa ataagctgaa aggccttggg
    46621 cagacgcaaa acctgcaaga gtcctagccg tatggttagg taaatccctg ttccgaccaa
    46681 taaaatcaag aggggcggtc cccaagcaaa agcatcgatt gatttaagca attctaacat
    46741 ttccttctcc tatcgtttca accccaaaag aaagagcaca tgcaagatac atgtactctg
    46801 gaatgcttag ataaatgcta aaaagcggtc tatcctagct ctgtcctttt acctgagagt
    46861 ttgagcagtt gcctgccttg ccccttcggt gcctttacgg tctctccaga gttccgtcca
    46921 tttacagtca tggaaaatca aacgattccc cacttctatt aaacttcatt cggtgttggt
    46981 atttaattga ttctaatttt acaaaaaatg ttggcttttg tcaatgtgtt tattagtaaa
    47041 aattagttca acagttttta ctttataaag tccagaatac tgctatcctt taaaagtgac
    47101 aatagtcgca ccactgcctc cagcattttg tggggcatag ccgaaactct tgacatgttt
    47161 gtttctttgc aagtatttgg taactccttc acggatgact cctgttccga taccatggat
    47221 gatatcaact tgagccatat tgttaagcaa ggcttggtcg atgaaggtat ctagctcatt
    47281 catggcttct tcatagcgct tgcctcgaag atccagtcta gcttgaggtc ctcgcccaga
    47341 agttcgtttc acaacattga cctgtttctt cttgactggt ttttcttgct gggcttgaac
    47401 aagatcaaac tctttctctt ccaaggtcat cttaatcaag ccaacttggg cttcccagcg
    47461 gccgtccttg agttgactgg tcaaggtacc acgctgacca taactgagaa ccacgatatc
    47521 atctcccacc tttggagctc gttttttctt ggccttttga aggaccttgt ttttagacaa
    47581 gtccactttt tcaggagcca attttttcaa cttggccttg gcttcaatga tttcgtgggg
    47641 cttgagttgg gatttactgt ggagattttt gagaatctgg tcactttcac ttagggccat
    47701 atccacaatc tcagcagcct gttcacgcgc cttgttaagc tcggtttcct tttcacgatt
    47761 aagctcgttg tagagttttt ttagcgcacg gttcatcttg agattttctt gctccacctc
    47821 acggatattg tccaagcgtt tgcggctttc cagcgtctgc tcttctaatt gctcaatgat
    47881 acgattgacg tcattgtcct gatcgatctg ctgactggca tctcctacga taacttcaga
    47941 taggcctaga cgtttggcaa tttcaaaggc attacttcgg ccaggaacac cctgcataaa
    48001 gcgataggtc gggcgaagag ttgcagtatc aaactccata ctggcatttt gcacaaaggc
    48061 tgtctcaata ccgtaggcct tgagttctgg atagtgggtc gtcgccatgg tcttgatttg
    48121 acgcaggcga aggtcctcca gaatagccat ggcaagggct gctccctctt ggggatcagt
    48181 accagccccc aattcatcca aaagtaagag tgaatgttgg ttgaccttgc caagaatatc
    48241 cacgatattg gtcatatggc tagagaaggt agacaagctc tgctcaatag actgctcatc
    48301 tccaatatca gaaaaaattt cttcaaaaat accaacacga cttcccttgt ctgctaaaat
    48361 cggcaatcct gactgggcca tgacctgtgt caagcccaga gttttgagca tgatggtctt
    48421 cccacctgta ttgggacctg taatgacaat agctgttaaa tcttgaccaa aatagacatc
    48481 atttgcgacg gcatttttga ccaaaggatg gcagacatgg agcagttgaa tctcttgatt
    48541 ttctgacagc tgaggcacga ctgcttgtct ttcttggata aatcgaacct tggcacgaat
    48601 caagtccaga tgaccgataa tccaagcatc attagcaatc tcagccgcat gagggcggac
    48661 acgctcagaa atttcttgga gaatgcgaag catttcatag cgttcatctg ctcgcagact
    48721 agcaatttct tcgctcagtt tgactacctc acgtggttcg atatagacgg tgtttccact
    48781 agcagaaata tcatgaacga cacctgcaat cttattgcgg taggtgtttt tgactggtaa
    48841 aacctgacgg ccatttctgc tagcaacaat tccttccgtc aacatctgcg ctttttgctt
    48901 gagcaagtct tgtaaaacat cgcgtacctg actctcgcta tcatgtattt ttcgacggat
    48961 tcgcgccaat tcttcactgg caaaattttc aatgaaaccc gcatcattaa aggcctgaag
    49021 atttccttgt aattgcggaa aatcatgtaa tttctcaaac caaagggcta attcttccaa
    49081 gctgacattt tccagattgg cgtaaaaact ttgaagttct cggctggcaa gaagcacgcg
    49141 tttcaagagt aggaactcct cgatattgag atccgctccc atctccaacc tcttgcagac
    49201 tcctgcaatt tccttagttg agagaatagt aaaatgcggt tgctcgacga aaagagcctg
    49261 catttccttc atctcagcaa aagcctgttt gattttatct gctttggcag tcggagccag
    49321 ttgtctcaat tgctccaagc cctgctcggt caacaaatga ggctcaaaca aggccttgac
    49381 cttatcgaac tctaatgttt ctaatatttt cttattcatc tttatttcct tgtctttgaa
    49441 tgtctaggtg tggtagcttt ttacattctg atagattcga gtcaaatctg taaacaaagg
    49501 gctaggaaaa ctcctgccct ttttatccga ttaaatttgt cacccagatt tgtttgagcc
    49561 aactggttgt tatcggtatg ctctggatga tgtgttttgc gacgatactc ttttcaagag
    49621 gattttgtat aactgccatg gggatggtcg ccaagattgt caaggccatt tgcaagacaa
    49681 ataaggtcac caacatggac aagatacctg ctgaaacttg gaacaactta ccacccagtt
    49741 ttttgctagg aatcaagtgt aagagaagac caagcaaacg accaatgcta tagacaatcc
    49801 caaatacaag caagtagccg atgcctgcat aaaagacctt atccagctga aagagttgat
    49861 ccgatgggaa aaagaaagtc ccctgacctt cctgcgaatt tgcataaggg agcaataaat
    49921 ggaattgctc tccaagcccc ttataaaact ggccagccat aaaagccgat gccatggctg
    49981 aaatcaggta ataaacctgt aagagcaggc ctctccgata gccgatataa aatccccaaa
    50041 ccaagaccaa tagaagaagg aatgaaatca taaggaatcc tcaatcttgc tctgttcttg
    50101 cttacaagtc acaagcttgt gacggagttc ttctagctct tgctccttat cgtcaaattc
    50161 aatctcacgg ctgagctgag ttgataaaca gttgactgcc aacaaaagag cgattgtttc
    50221 atcatctgcg ctaggcattt gttctttaat tgcttggtat ttttctgtcg caaccttagc
    50281 gatttcctcc ataaaaaggt tgtcatgttc gcttgtcaag gttaacgatt ttttcccgaa
    50341 tgtaaatttg aatcgattta gatttgccat aaaattcacc tcacgatatt ataccaaaat
    50401 tcgctaattt tgtcagtttt tacaaatttt actgcttttg tggtacaata gaaactatgg
    50461 caagtatcac actcacacca agcgaaaagg atattcaggc ttttcttgaa cactatcaaa
    50521 ccagtctggc tcctagcaag aatccctata tccgctactt tttgaaacta cctcaagcaa
    50581 cggtttctat ctatacttct ggaaaaatct tgcttcaggg tgaaggggct gaaaaatacg
    50641 ccagtttctt tggctatcaa gctgtagagc aaaccagcgg acaaaatctt cctttgattg
    50701 ggacagatga ggtagggaat ggttcctact ttggtgggct tgcagttgtg gcttcctttg
    50761 tcacacctga ccagcatgcc ttcttacgaa aactcggtgt gggagattct aagactctga
    50821 ccgaccaaaa gattcgtcag attgctccta ttctcaagga aaaaattcag caccaggcac
    50881 tccttctctc acccagcaag tacaacgagg tcatcggaga ccgctacaat gctgtttcgg
    50941 ttaaggttgc cctccataat caggctatct atctcctcct tcaaaaaggt gttcagcctg
    51001 agaaaattgt gattgatgcc tttaccagtg ctaaaaatta tgacaagtac ttggcacaag
    51061 agaccaatcg tttcagcaat cctatcagct tagaagaaaa ggctgagggc aaatacttgg
    51121 ctgtcgcagt ttcttctgtc attgcgcgtg atctctttct ggaaaatctt gaaaatttgg
    51181 gacgagaact gggttatcag ctcccaagtg gagctggaac ggcttctgac aaggtggcta
    51241 gccagatttt gcaagcctat ggtatgcagg gactcagctt ctgcgccaaa ttgcacttta
    51301 aaaacactga aaaagcgaaa aaacgcttag aaaggtaagt tatgaattca tttaaaaatt
    51361 tcttaaaaga gtggggattg ttcctcctaa ttctgtcatt actagcttta agtcgtatct
    51421 ttttttggag caatgttcgc gtagaaggac attccatgga tccgacccta gcggatggcg
    51481 aaattctctt cgttgtaaaa caccttccta ttgaccgttt tgatatcgtg gtggcccatg
    51541 aggaagatgg caataaggac atcgtcaagc gcgtgattgg aatgcctggc gacaccattc
    51601 gttacgaaaa tgataaactc tacatcaatg acaaagaaac ggacgagcct tatctagcag
    51661 actatatcaa acgcttcaag gatgacaaac tccaaagcac ttactcaggc aagggctttg
    51721 aaggaaataa aggaactttc tttagaagta tcgctcaaaa agcccaagcc ttcacagttg
    51781 atgtcaacta caacaccaac tttagcttta ctgttccaga aggagaatac cttctcctcg
    51841 gagatgaccg cttggtttcg agcgacagcc gccacgtagg taccttcaaa gcaaaagata
    51901 tcacagggga agctaaattc cgcttctggc caatcacccg tatcggaaca ttttaagaaa
    51961 cctaagaggc cgagaatcac caatctcagc ctcttcttct atcgtgagaa aatgattggt
    52021 aactatctaa acttaccaga acagaaacac ctcaactctc acctattcat gcaaaggaat
    52081 tttatggaag tttatttttc aggaaccatt gaacggatta tttttgaaaa tcccagcaat
    52141 ttttatcgca tcctcctcct agaaatcgac gatacggacg cagaggattt tgatgatttt
    52201 gaaatcattg tcacaggaac catggctgat gtaattgagg gcgaagacta tactttttgg
    52261 gggcaaattg tccagcactc caagtatgga gaacaactgc aaatcagtcg ttatgatcgc
    52321 gcaaaaccaa ctagtaaggg cttggtcaag tacttttcaa gtagccattt caaggggatt
    52381 ggtctcaaga cagctcagaa aatcgtggat acctatggcg aaaataccat tgacgaaatt
    52441 ttgcaacacc cagaaaagtt agaaggcatc acaggactct ctgccaaaaa tcgcgaggct
    52501 ttcgtctcca ctctccgtct caactacgga acggagatgg ttttgtccaa actagccaac
    52561 tacggcattc ccaacaaact agcctttcag attcaagact tttacaagga agaaaccctt
    52621 gatgtggttg aaaattatcc ctaccagctg gttgaggata tcaagggttt gggctttacc
    52681 attgctgacc aactagctga ggaactaggc atcgaaagtc aggctcctga acgcttccgc
    52741 gccggtctag ttcacagtct ttttcaggcc tgtatggaaa caggggacac ctatgttgaa
    52801 gcacgggatt tgctggaaca aacccttact ctccttgagt cttcccgtcc cgtggaacta
    52861 gaccccagcc aagttgccca agagctctcc tacctgatcg aagaagacaa ggttcagcag
    52921 attgatacca agatctttga caacagcctc tttttcgctg aggaaggcat ccgcagtcac
    52981 ttggttcgta tccttgaaaa aggaaaacag aagagtcatg atttagaaac cattcaaaaa
    53041 catatcacta ctatcgagga agagctggga attgagtatg ataacattca aaaacaggct
    53101 atctgtgatg ctatccagaa taaggtcttt atcctgacag gtgggcctgg tactggtaag
    53161 acaactgtta tcaatggaat cattgctgtt tatgcccttt tagaaggact tgacttcagg
    53221 aagaaaagca atctgcccat tcttcttgct gctccaactg gtcgagctgc tcgtcgcatg
    53281 aatgaattga caggtttgcc tagcgcgacc atacatcgcc acttgggaat gacaggtgac
    53341 gatgatacca gtcatctgga agattaccta gatgctgagt ttatcatcgt ggatgaattc
    53401 tctatggtag atacttggct ggccaatcaa ctcttctcca atatctcttc taacagtaag
    53461 atcctcatcg taggtgacag tgatcagcta ccttctgtca gtcctggaca ggttctagcg
    53521 gatctgcttc atattccttt gattcctcag actcgcttgg aaaaaattta tcgacaaagc
    53581 aaagaatcaa ccatcgtcac cctagctagt cagattcgac agggcatctt gccagctgat
    53641 ttcacccaaa aaaaagctga ccgttcctac tttgaaattg ctagtggcca tattcctgcc
    53701 accattgaaa aaatcttagg tgccgccctc agaaatggta ttcccgcccg tgatatccaa
    53761 gttctggctc ctatgtaccg agggacggca gggattgatg ctatcaatca gctcatgcaa
    53821 gacctgctca atcccccaca aaaagatcaa ctcagttttg aagctcctca gtgtcactat
    53881 cgtaagagag acaaggtcat tcatttggtt aacgatgctg aaatcaatgt ctttaatggg
    53941 gatttaggag ctatcacaga cctgattcct ggtaaataca ccgaatcgaa acaagacgag
    54001 attgtcattg attttgatgg taatgaagtc tcttaccccc gtaacgaatg gtacaagatt
    54061 cgcttggcct atgccatgag tattcataag tctcagggaa gtgagttccc agttgtcatc
    54121 ctaccgatta ctagtgctag ccgacgtatg ctggagcgaa atctcatcta cacagccatt
    54181 acacgtgcca aaagcaaact catcttacta ggcgaattac aagccttcga ctatgctacc
    54241 caacacatcg gaactgcccg aaaaacctat ctgattgaac gcttcagtga tctactggag
    54301 aatgttgaag aaaagcaaca agctgtctct gaaacagtca catcaagtgc ctctgaacaa
    54361 tcctacatcc taaccgaaga aaactgggac cgcatcccag ccatgattgg gattacagac
    54421 acagacctca aagagatttt tggaaaatag tgcatcagaa aacccaccaa ttcagatggg
    54481 ttttctgttt attaagatta ttttactgtt gctgtgcttg taatcaattc aacagctttt
    54541 ttgattgtga tatcatgttt caacatgtca gctgaaagca agctttgtac ttgagcaact
    54601 tccatgttgt agtctgctgc caattgctca acttcttttt ggatttcttc ttctgaagca
    54661 tcaaatcctt cagctttggc aactgcttcg ataacaaggt tagtcttagt acgtgactca
    54721 gcttctgctt ggtattggtt gtgaaggtct tcttgagtag ttccagtgat ttggaagtac
    54781 atgtcagggt tgatcccttg acgttgcaag ttcccaagga attcatttac tgaacggtga
    54841 acttcttcgt ggatcatttc ttctggaagt tctacgattt cagcattttc tacagctgta
    54901 tcaattgctg caccttcaac tgcatctttg taagcttctt ctttagcagc agccaattct
    54961 ttgcggtatt tttctttcaa gtcagcaagt gtttcaactt cttcatcaat gtcttttgca
    55021 agttcatcgt caagagctgg aacttcttta gcttttactt catggatagt tgtcacgaat
    55081 ttagcttctt tacctgcaag gtcttctgct tggtagtctt ctgggaatgt tacgataaca
    55141 tcaacggttt cgccagctga gtgacctacc aattggtctt cgaaaccagg gatgaattga
    55201 cctgaaccaa gtccaagtga gaagttttca ccttttccgc cgtcaaattc aacaccgtcg
    55261 atagaaccaa cgaagtcgat cacaacagtg tcgccgtttt cagcagcagc ttccttgata
    55321 accaattcag tcaggttgtt gcgttcgcgt tcgatacgct cttcaacatc agcgtcagtt
    55381 acttcttttt ctacatcaac tgatacttca aggtttttgt agtcacccaa ttttacttca
    55441 ggttttgtaa cgacttcagc agcgataacc caatcttgac ctttttccat tgaagttacg
    55501 tcaatttttg gttgggcaac cacttcaaga ccagcttctt ttacagctgc ttcataagcg
    55561 tttggcaaaa gagcgttcat aacgtcttgg taaagtgatt cttcaccaaa ttttttatcg
    55621 aagataggac gtggaaggtg acctttacgg aaacctggaa cattaagaga tttcttcact
    55681 gacttgaaga cacggtccaa ttctggtttg atttggtctt gagagatagt gaaagtcaag
    55741 acaccacggt ttgtttcttt gttttcaaat gatacagaca ttctgtcatt tctccttaaa
    55801 attttttaaa tacagtctat tataacattt tctataaata aaatctagac taatgcagcc
    55861 aattatagac aacaagcctt tttcctaact aaaacactca taaagtatag acaattttag
    55921 actactttgg atatcttttg gttagtcatt ttccaaaact aatttatcta atttttcagt
    55981 tatatagtag attgaaacta gaatagtaca cctctacttc taaaacattt ttagaaatcg
    56041 atttgactgt cctgatcgat ttgtcctgtt cttgtttcat tttactatat agatcgcatt
    56101 cgaaagaatt cgttccaaaa ttgcttggtt cgttcttctc ttggacgact aaagaattga
    56161 tgggcattat ttttttcaac aattttccca ccgtccataa aaataacatg atgggctaca
    56221 tctctagcaa aacctatctc ataaagctga tcaaaaactc aatctgagga gaaaatccaa
    56281 taaggctttc cataataaag gacatcctat caagattttt gaacacccga taggctgcct
    56341 ttttccgatt ttgaagactt tttgtccagc ttcttttacc ttataaataa caacaaaaat
    56401 caaagacagg ctagaaagat taccgcagat aactcaaaca gtgttttgag gttgcagata
    56461 agactgacga agtcagctca aagcactgct ttgaggttgt agatagaact gacgaagtca
    56521 gctcaaaata ctgttttgag gttgcagatg gatgctaacc tgatttgaag agattttcga
    56581 aaaagattaa atatactcta caatttgctc aatcaattct tggtcgtcta aacctgtaac
    56641 ttgtataaca acatctttga ctgattgttt agccaacaat tcacctaatc gaatactttc
    56701 ttcatcattt acatcgcgat agtcaaaggc atagccaact gttttaagta ggtttttata
    56761 tgacaaactg agttctttca attcacgtat cggccggatg aatcgttcat tatagcctaa
    56821 ttttcggatt ggagtacgag ctacgcgact aacctcgtcc actatgaaag gattttcaaa
    56881 ccgttctata atgactttgt gataattctc caattctttt ttatcaaagt tccatttggc
    56941 aatcaagaga ctccgaattt cagctaatac agattcaatc cgagatttga tattaggatt
    57001 ttgaagagct tccaaaattg tcttggcacc ataatgcgca ccaatgtaag ctgaagttgc
    57061 atgtccagaa ttgactgaaa aaagttttcg ctcaataaag ggttctaaat cttcttcata
    57121 atgcacatct tttagacgta aatctggatt tttaagacgc ttggtttcca cgacccattc
    57181 attaaagggc tcgaccacaa caaaaaggga atcttcgtga ctttgtgctg gaacaatcct
    57241 gtctactgca gcatttggaa aacctatgta gttatcagca aatgtcaaac cttccggact
    57301 taaatatttc ttgacttctt gataaagaaa ttgagacccg ccaatcatat tttcacaggc
    57361 cataacatcc aatgcctgtg tatttcctgc aactcggcga gcttcgattc ctttggctag
    57421 aagttcggcg ataaaaggga gtatattagg tccgattgca gtagtaataa tatccgtctt
    57481 ttgaatcgct tcaatgactt gctcaggatg ttctttgcta ttaatgccag ccacattagt
    57541 tacttctata cgagactgtc ctttctgtgc aatttcaatt tcatacttgc ccttttcatt
    57601 tagagcatga attatctgat tattgacatc cacaaaatca atatggaaac catttttaaa
    57661 tagaatttca cctataaaac cacgaccgat attaccggca ccaaaatgaa cagaatgttt
    57721 cattcaattt ctccttttcc tatttgaggg acatatatac tagatgacaa atgacgaaat
    57781 tcaattattc aacagcatca aataagcgca atacttcctc ttttgactga gcatctgcta
    57841 gtttaagaac attatctaca tctgcacaga agatagaaat tttctgaata atttctaagt
    57901 gttcattacc aataccagca ataccaaaaa gaaccgttgc cacttgaggg ttagatacat
    57961 tcccaaaatc aacccctcta gggacttgaa cgactgtaat accagacttg aggacatcat
    58021 tttttgctgc atctgtccca tgcggtatgg cgataaagtt acccatgtaa acagatagct
    58081 ctttatctcg ctcaatcatg gcttcaatat agtcttcatt aacatatcca ccctcataaa
    58141 gaacttgccc acaataacaa atagcttcct ccttgttaga aaattgttta ttaagcttaa
    58201 tcaaatgttt ttcaagtttc atattagttc tccaatttct taattttttc gttaaaaata
    58261 gtatttagca tctgataaat aatggattga tttcccgtct tgtaaatctc tgtataaaga
    58321 tgattttcaa taatcgactg actaatagct gtcattaaat ctctcgcttc ctcgctagct
    58381 tctttagaca ttagcattag cagacacctt ttgacctctt cttcctctag tttcattgac
    58441 aatgcggaga taggtagttt gagttcaaac atagtaaaac tatttgttgt tactttgcta
    58501 gactgactat gtaaaagcac cagaccagta ttaggaatag ccataggact attctggaag
    58561 tgagataaca attttcttgt taggtaatct ctgtcactca cattcttctg atattggatg
    58621 atttgttcta ccgtgtgctc aaatgattga ttattctcca attgaaccaa ctcgaattga
    58681 ctcaaaagct gactactaga tattaaataa tcttgcaagt cataactttt ctctggagcg
    58741 atagcatcac gcgcgacaat tgtacgatgt gcctgtacat actgaagtcg ttcctgtaat
    58801 tcaagcaatt ctttgacggt tggaaaacta gaaattacat cgatttcctg attagtcagc
    58861 ggcttggtag ataaaatata atcatactga ctgagatcaa tactgtggta atctactaga
    58921 gactgaatat ctatcaactc tacaaaagga gcaacactct taattttact gactaataaa
    58981 tctctagtga cccgacgttc atccgttaaa agcaaaacac ggactggata aatctctgga
    59041 ctacggccta agctagagat aaaatgtagg gcaatcatgc catactcata ctctgtatga
    59101 agatatttcg gaaaaatatc attcactaaa agactgatga ctgtataaag aaatttattc
    59161 ctttcaacta aaatctggac agattctggc aaattttcac cctgaaaaag gatagggacg
    59221 cctaaactga gccgaatatg atggaaaaga aaattgaata aaaccttgtc cttaaaaaag
    59281 tcaatcttgg tatacatgga aaccgtatca atcagatttg aaatattgta gaaaaattca
    59341 ccatcaaatt tctccgtaaa gagcggattg tcctgacgtt taatgattaa ttcatccaag
    59401 atgctcgcaa aatagataat ttcctgaata ctataaaatt gtgcagtctg cttagagtaa
    59461 gtttgaaaaa ttttttgaga aatttccaaa gcctgcttac tagtattagg tgaattttca
    59521 atgttttgct cctgacctat aagagataac aagatcgcaa aaaacatctt catcctagca
    59581 tccatatctg gaaaacctga cagttgctta ttaacaatct gactggccag cctagttcta
    59641 tctgcttcta aaatatcaaa gctcccaaaa ttaccggttg aaaaatctgc tactgagata
    59701 cagtttgtca gtaaaatagc caaaaatctt ctcttaccaa ctgaatcacc agaaatccga
    59761 taaccttttt gtcgttcaat tttcagatca aagtctaaaa gacgcttatc aatatctgaa
    59821 atatcctgaa taatagttac attactgact ttcgtgcact cttgcaatgc ttcattggtg
    59881 acaaaaccac tctcagtcag aaggcgatag gtaatcaact cttgtctttc ttggggacta
    59941 tactcaacta acacttcaag ttctgtcggc aaatcatcca actctccagt caaaatatag
    60001 tatttcccat gcttcgttat ttcgattcca tattgctcca tgctatctgt caaatctgat
    60061 aaagttcgat aaattgttct agatgaaacc tgtaagattt cagtcatatc ttgcattgaa
    60121 agcttcccta catgtaggaa agccttcaat aattgttctt ctcgtttggt taatagcatt
    60181 cgtaatttag aaacctctag ttctatttgt acattctagc agccattttg tcatattctg
    60241 gtgttgttac taaactatcc acaataagaa attgagaatt cggtgctgct tgctgcactt
    60301 ctgcctgtaa agaaatagtt gttacaatca ttatgttttt agaattaaat tcacctaatt
    60361 ctgaaatttt ggcagtagaa actggaatac gaatattctt gtttctaaag atagcccgaa
    60421 tcgtttcaca gcccatagtt gcagttccct gtgctttacc ataagcaact actacagcat
    60481 caatatggtt aaggtcaatt tcctgactat ctactggtgc tgaagttggt atatctcctt
    60541 caatttctgc tattggagaa gctcctgtta atgaagctac aatttcatca taacgagggg
    60601 acgctaagaa attatcaaca gaaacatgaa tagcacttgg actcttgtct ttagctcttg
    60661 gtgtcagttc ttcctgagta acaattaatg tttttggtgt atcaagcaaa tttgagattg
    60721 cctgattaga tactggaatc tctagacctg cttttttaac cttatctcga agaatactag
    60781 ctcccatagc agagcttccc ataccagcat cgcaggcgaa aatgattttt tccactgagt
    60841 ctgtcgaaac aactgcatca acagaagttg atactaactg acctttagac tgagccttag
    60901 ctgctttggt agccgcctga gcagcttcga gcgaatcctc agttgacttg tctgcatgaa
    60961 gaataagggc tgctacaagg aaagaaacaa ctgctgctac taaaacacct agaagaacat
    61021 ttatatgggg ccaaacacct tttggcgccg tagccataat cgcaataata gaacctggtg
    61081 aagctggaga tttaagacca gcgtctaaga gttgaaaagt aaaagttcca gagatacctc
    61141 ccgccatagc agctaaaaat agagtaggct tcatcataac ataaggaaag taaatttcat
    61201 gaatccctcc gaagaaatga ataaccattg ccccccaaga agaagattta gcagaacctt
    61261 taccgaatac agcataagct aatagaattc ccaggcctgg tccaggatta gcttccaata
    61321 ggaagagaat tgacttacca gcttgagcta cctgttctac tcccagagga gtaaaaatgc
    61381 catgattgag ggcattattg aggaaaagga ctttagccgg ttcgatgata atattagcca
    61441 taggaaggag gcgagcattg acaatagcct caacaccatt cccaacagct ccagtaagag
    61501 tcgatacgac tggaccgatt gcgtagaaag ccaaaagcaa taatgcaaaa ccaacgagac
    61561 cagctgagaa gttattaact aacatttcaa atccgggacg aattttttct tggaacttct
    61621 catcaaattt cttgatagtc catcctccca gtgggcccat taccatagct ccgataaaca
    61681 taggaacact agaacctgta attgcaccaa cagtagcaat agctcctaca acggcaccac
    61741 gttggccatg gatcatatat ccacctgtgt aaccaatcag gattggcaat aaatacgtta
    61801 acataggacc aacaacagta gctaactgtt catttggcag atagccatca gcgataaaga
    61861 gggcagtcaa tactccccaa gcaataaatg ctccaatatt gggcataacc atatttgaaa
    61921 gcgatgtccc tagtttttga accctgactt tcaatgatac tttttcttcc attagatcat
    61981 cctccttttc ttaaaactag tatatacaaa gttaaaaacc tttaaaacta aagttttgtc
    62041 acagattttg tgtcaaaaca ttacttttga cgaggctggg caaaaaggct taaaaattat
    62101 aaaacgcatc atatcaggtg tacatgaact acaccccaaa agttagacag aaaaaatcta
    62161 acttttgggg tgtttttatt atgaaattaa cttatgatga taaagttcag atctatgaac
    62221 ttagaaaaca aggatatatc ttagagaagc tttcaaataa atttgggata aataattcta
    62281 atcttaggta catgattaaa ttgattgatc gttacggaat agagttcgtc aaaaaagg
//
LOCUS       DAYYQN010000004        56784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYYQN010000004 DAYYQN010000000
VERSION     DAYYQN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.004"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            74..1677
                     /locus_tag="ACHBRQ_000228"
                     /pseudo
     CDS             74..1677
                     /locus_tag="ACHBRQ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(1707..4259)
                     /locus_tag="ACHBRQ_000229"
     CDS             complement(1707..4259)
                     /locus_tag="ACHBRQ_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9591971.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMGFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIRFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4321..5943)
                     /locus_tag="ACHBRQ_000230"
     CDS             complement(4321..5943)
                     /locus_tag="ACHBRQ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9591972.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            6149..7174
                     /gene="trpS"
                     /locus_tag="ACHBRQ_000231"
     CDS             6149..7174
                     /gene="trpS"
                     /locus_tag="ACHBRQ_000231"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9591973.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFTKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            7326..8804
                     /gene="guaB"
                     /locus_tag="ACHBRQ_000232"
     CDS             7326..8804
                     /gene="guaB"
                     /locus_tag="ACHBRQ_000232"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9591974.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(8855..9952)
                     /gene="recF"
                     /locus_tag="ACHBRQ_000233"
     CDS             complement(8855..9952)
                     /gene="recF"
                     /locus_tag="ACHBRQ_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9591975.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(9955..10323)
                     /locus_tag="ACHBRQ_000234"
     CDS             complement(9955..10323)
                     /locus_tag="ACHBRQ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9591976.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            10482..11732
                     /locus_tag="ACHBRQ_000235"
     CDS             10482..11732
                     /locus_tag="ACHBRQ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9591977.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            11729..13012
                     /locus_tag="ACHBRQ_000236"
     CDS             11729..13012
                     /locus_tag="ACHBRQ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9591978.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13054..13875
                     /gene="rodZ"
                     /locus_tag="ACHBRQ_000237"
     CDS             13054..13875
                     /gene="rodZ"
                     /locus_tag="ACHBRQ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFM9591979.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            13886..14431
                     /gene="pgsA"
                     /locus_tag="ACHBRQ_000238"
     CDS             13886..14431
                     /gene="pgsA"
                     /locus_tag="ACHBRQ_000238"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9591980.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            14428..15255
                     /locus_tag="ACHBRQ_000239"
     CDS             14428..15255
                     /locus_tag="ACHBRQ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9591981.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRREWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            15240..16079
                     /locus_tag="ACHBRQ_000240"
     CDS             15240..16079
                     /locus_tag="ACHBRQ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFM9591982.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16072..16866
                     /locus_tag="ACHBRQ_000241"
     CDS             16072..16866
                     /locus_tag="ACHBRQ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9591983.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            16925..17743
                     /gene="mreC"
                     /locus_tag="ACHBRQ_000242"
     CDS             16925..17743
                     /gene="mreC"
                     /locus_tag="ACHBRQ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9591984.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            17743..18237
                     /gene="mreD"
                     /locus_tag="ACHBRQ_000243"
     CDS             17743..18237
                     /gene="mreD"
                     /locus_tag="ACHBRQ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9591985.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            18331..19509
                     /gene="pcsB"
                     /locus_tag="ACHBRQ_000244"
     CDS             18331..19509
                     /gene="pcsB"
                     /locus_tag="ACHBRQ_000244"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HFM9591986.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            19733..20512
                     /gene="rpsB"
                     /locus_tag="ACHBRQ_000245"
     CDS             19733..20512
                     /gene="rpsB"
                     /locus_tag="ACHBRQ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9591987.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            20591..21631
                     /gene="tsf"
                     /locus_tag="ACHBRQ_000246"
     CDS             20591..21631
                     /gene="tsf"
                     /locus_tag="ACHBRQ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9591988.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            21878..22201
                     /gene="tnpB"
                     /locus_tag="ACHBRQ_000247"
     CDS             21878..22201
                     /gene="tnpB"
                     /locus_tag="ACHBRQ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9591989.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLSYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENVRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(22356..22664)
                     /locus_tag="ACHBRQ_000248"
                     /pseudo
     CDS             complement(22356..22664)
                     /locus_tag="ACHBRQ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22646..23175
                     /locus_tag="ACHBRQ_000249"
                     /pseudo
     CDS             22646..23175
                     /locus_tag="ACHBRQ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23250..24170)
                     /gene="cysK"
                     /locus_tag="ACHBRQ_000250"
     CDS             complement(23250..24170)
                     /gene="cysK"
                     /locus_tag="ACHBRQ_000250"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9591990.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(24455..25090)
                     /locus_tag="ACHBRQ_000251"
     CDS             complement(24455..25090)
                     /locus_tag="ACHBRQ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9591991.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            25146..26444
                     /locus_tag="ACHBRQ_000252"
     CDS             25146..26444
                     /locus_tag="ACHBRQ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9591992.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            26441..27103
                     /locus_tag="ACHBRQ_000253"
     CDS             26441..27103
                     /locus_tag="ACHBRQ_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9591993.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            27183..27731
                     /gene="raiA"
                     /locus_tag="ACHBRQ_000254"
     CDS             27183..27731
                     /gene="raiA"
                     /locus_tag="ACHBRQ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9591994.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            27867..29840
                     /locus_tag="ACHBRQ_000255"
     CDS             27867..29840
                     /locus_tag="ACHBRQ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9591995.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            29837..30289
                     /gene="rplI"
                     /locus_tag="ACHBRQ_000256"
     CDS             29837..30289
                     /gene="rplI"
                     /locus_tag="ACHBRQ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9591996.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            30333..31685
                     /gene="dnaB"
                     /locus_tag="ACHBRQ_000257"
     CDS             30333..31685
                     /gene="dnaB"
                     /locus_tag="ACHBRQ_000257"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9591997.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKRLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            31687..31953
                     /locus_tag="ACHBRQ_000258"
     CDS             31687..31953
                     /locus_tag="ACHBRQ_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9591998.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            32042..33388
                     /gene="cbpD"
                     /locus_tag="ACHBRQ_000259"
     CDS             32042..33388
                     /gene="cbpD"
                     /locus_tag="ACHBRQ_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HFM9591999.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      33471..33573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33644..33934
                     /locus_tag="ACHBRQ_000260"
     CDS             33644..33934
                     /locus_tag="ACHBRQ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFM9592000.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            33897..34655
                     /locus_tag="ACHBRQ_000261"
     CDS             33897..34655
                     /locus_tag="ACHBRQ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592001.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            34693..35700
                     /locus_tag="ACHBRQ_000262"
     CDS             34693..35700
                     /locus_tag="ACHBRQ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9592002.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            35700..36428
                     /locus_tag="ACHBRQ_000263"
     CDS             35700..36428
                     /locus_tag="ACHBRQ_000263"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592003.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AETISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            36547..37005
                     /locus_tag="ACHBRQ_000264"
     CDS             36547..37005
                     /locus_tag="ACHBRQ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9592004.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLVSVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            37007..39439
                     /locus_tag="ACHBRQ_000265"
     CDS             37007..39439
                     /locus_tag="ACHBRQ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9592005.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLMTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            40081..40734
                     /gene="rr06"
                     /locus_tag="ACHBRQ_000266"
     CDS             40081..40734
                     /gene="rr06"
                     /locus_tag="ACHBRQ_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HFM9592006.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            40731..42062
                     /gene="hk06"
                     /locus_tag="ACHBRQ_000267"
     CDS             40731..42062
                     /gene="hk06"
                     /locus_tag="ACHBRQ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HFM9592007.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDENGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            42157..42684
                     /locus_tag="ACHBRQ_000268"
     CDS             42157..42684
                     /locus_tag="ACHBRQ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFM9592008.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            42763..45573
                     /locus_tag="ACHBRQ_000269"
     CDS             42763..45573
                     /locus_tag="ACHBRQ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HFM9592009.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGIT
                     QVATSYNKANESQTEHRKAAKQVDEDIKKMLSEIQEYIKKMLSEIQLDKRKHTQNVNL
                     NRKLSAIQTKYLYELRVLKEKSKKEELTSKTKKELDAAFEKFKKEPELTKKLAEAKQK
                     AKAQKEEDFRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKPRNEEKIKQAKAKV
                     ESKKAEATRLEEIKTERKKAEEEAKRKAEESEKKAAEAKQKVDTKEQGKPKRRAKRGV
                     SGELATPDKKENDAKSSDSSVGEETLPSPSLNMANESQTEHRKDVDEYIKKMLSEIQL
                     DRRKHTQNVNLNIKLSAIKTKYLYELSVLKENSKKEELTSKTKAELTAAFEQFKKDTL
                     KPEKKVAEAEKKVEEAKKKAKDQKEEDRRNYPTNTYKTLELEIAESDVKVKEAELELV
                     KEEANESRNEEKIKQAKEKVESKKAEATRLEKIKTDRKKAEEEAKRKAEESEKKAAEA
                     KQKVDAEEYALEAKIAELEYEVQRLEKELKEIDESDSEDYLKEGLRAPLQSKLDTKKA
                     KLSKLEELSDKIDELDAEIAKLEVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAE
                     ADLKKAVDEPETPAPAPQPAPAPEKPAEKPAPAPAPEKPAPAPQPAPAPEKPAEKPAP
                     APAPEKPAPTPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGW
                     LQNNGSWYYLNSNGAMATGWLQYNGSWYYLNANGDMATGWFQYNGSWYYLNANGDMAT
                     GWFQYNGSWYYLNANGDMATGWFQYNGSWYYLNANGDMATGWLQYNGSWYYLNSNGAM
                     VTGWLQNNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGS
                     GALAVNTTVDSYRVNANGEWVN"
     gene            complement(45803..46783)
                     /gene="dusB"
                     /locus_tag="ACHBRQ_000270"
     CDS             complement(45803..46783)
                     /gene="dusB"
                     /locus_tag="ACHBRQ_000270"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9592010.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(46770..47642)
                     /gene="hslO"
                     /locus_tag="ACHBRQ_000271"
     CDS             complement(46770..47642)
                     /gene="hslO"
                     /locus_tag="ACHBRQ_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9592011.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            47807..49234
                     /locus_tag="ACHBRQ_000272"
     CDS             47807..49234
                     /locus_tag="ACHBRQ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9592012.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLYSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            49393..50901
                     /gene="glpK"
                     /locus_tag="ACHBRQ_000273"
     CDS             49393..50901
                     /gene="glpK"
                     /locus_tag="ACHBRQ_000273"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9592013.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            50943..52769
                     /gene="glpO"
                     /locus_tag="ACHBRQ_000274"
     CDS             50943..52769
                     /gene="glpO"
                     /locus_tag="ACHBRQ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9592014.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKRTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            52837..53541
                     /locus_tag="ACHBRQ_000275"
     CDS             52837..53541
                     /locus_tag="ACHBRQ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9592015.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            53780..53935
                     /locus_tag="ACHBRQ_000276"
     CDS             53780..53935
                     /locus_tag="ACHBRQ_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eukaryotic translation initiation factor 3
                     subunit E"
                     /protein_id="HFM9592016.1"
                     /translation="MVDMLVEFHKSQGTLETPEVQAEIAQKREEIEQRRAELEGKKQE
                     LLNRLNK"
     gene            complement(54022..54630)
                     /locus_tag="ACHBRQ_000277"
     CDS             complement(54022..54630)
                     /locus_tag="ACHBRQ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592017.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAMIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            54762..54959
                     /locus_tag="ACHBRQ_000278"
     CDS             54762..54959
                     /locus_tag="ACHBRQ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592018.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKNNKK"
     gene            55717..56784
                     /gene="ltrA"
                     /locus_tag="ACHBRQ_000279"
                     /pseudo
     CDS             55717..56784
                     /gene="ltrA"
                     /locus_tag="ACHBRQ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT        17885 a         9623 c        12356 g        16920 t
ORIGIN      
        1 tatatttaaa aaaggagaaa ctaagtttaa agaatggaaa gcaatttaaa aaaaaccaac
       61 ctttattatt gtcatgatcg ggatttctct tattccagat ctgtacaata tcatattttt
      121 gtcatcaatg tgggatccat atgggcaatt gtctgactta cctgtggcag ttgtaaataa
      181 tgataaagag gcttcctata atggtaatac tatggcaata ggaaaagaca tggtgtccaa
      241 tttaaaagaa aataaaacct tggattttca ttttgtagat gaagaggaag gaaagaaggg
      301 attggaagat ggcgattact atatggtagt gactttacca agtgatttat ctgaaaaaac
      361 aactacatta tccaatattc aatcgacagc agcttatcaa tcattgacaa gtgagcaaca
      421 aactgagata agtgattctg tatctcaaaa ttcaactgat agtattcaat cggctcagtc
      481 aattgtagct ttagtacaag atttacaggg aagtttagaa aacttacaaa atcaatcttc
      541 taatctttcg actttaaaaa atcaatctaa tcaagtatca cctattactt ctacttcttt
      601 gataggattg tcaagtggat taacagagat acaaggagat gttactagca aattagttcc
      661 tgccagtcag tcgattgcat caggtgtaaa cgcatatact acaggtgttg ataaagtttc
      721 tcagggcgca agtcaactaa gtgaaaaaaa tgccaccttg acaggtagtt tgaataaact
      781 agtttcaggc tcaaacacct tgacacaaaa atcttctaga ttgacagcag gagttggtta
      841 attacaatca ggatctgggt aattagcaga caaatccagt cagttacttt caggtgcttc
      901 tccattagag aatagagcta ataaattggc agatggatct gggaaactag cagaaggtgg
      961 aacaaagtta acttctggat tggaagattt acagacagga cttgcttctt taggacaagg
     1021 actaggtaat gctagtgatc aactcaaatc agtatcaaca gaatctaaaa atgcagagat
     1081 tttgtcaaat ccactcaatc tttcaaaaac agacaatgat caagttcttg taaatggaat
     1141 cgcaatagct ccttatatga tatcagttgc tctttttgtt gcagcaatat caacaaatat
     1201 gatatttgcg aaattgcctt caggacgtca tccagagagc cgttgggctt ggttgaaatc
     1261 tcgagctgaa ataaatggta ttatagctgt tttggcagga attttggtat atggaggagt
     1321 tcagcttatt ggtttaactg ctaatcatga gatgagaata tttattctca tcatcctaac
     1381 aagtttagta ttcatgtcta tggtgaccac tttaacaacg tggaatagcc gtataggagc
     1441 ttttttctca cttattttgc ttttactaca gttagcatca agtgcaggta cttatccact
     1501 tgctttgaca aatgatttct ttagatctat taatccctgg ttaccaatga gctattcagt
     1561 ttcgggatta cgacaaacaa tctccatcaa caagtcattt tcctagctgt catactagct
     1621 ctatttactg gtttaggtat gctagcctat caacataaga aaatagaaga agattaaaaa
     1681 aatcgaccga ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag
     1741 attccaaata gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga
     1801 atgaaggaaa gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt
     1861 tgagcttgtt taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa
     1921 ggaatggtga agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt
     1981 ttagaagttg atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt
     2041 ttggtatcta aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa
     2101 ctaacattga tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag
     2161 acattatcag ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt
     2221 ttatcgttag aaaagtgaag gtttatgaaa gagagataaa cttggctgtt ttctggaact
     2281 tcaatttgat actggattgc tgcatcttca tttgaagaac ttgtgacact aatcaaatca
     2341 ttagtatttt ctattttttc tgttttttca taaggtattg gagaaaaata atcaaaattg
     2401 acgttagcaa gttgatttaa aaatgaggcc tgattatcca aggtatgttc attgaacttg
     2461 acatcattgt aaacagattg actagcaact gcaatcggaa gagagtattg attttcatat
     2521 agggtaagat tatctttttg atagatatct ttaaagccat acttatcaat aggactgtct
     2581 gagatattgt actggatacc aaataaacta tcagccaaaa tactattatt tgcatatcgg
     2641 agattgagat tagtcccaga ggatttaaaa ccaagtttat ctaaagtaga gcttgatgaa
     2701 cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt tgaatttcat actgtcattt
     2761 cctgtctgag tttgtagttt ttcagtacga gtaaattgat ttccaatata tgttgagaaa
     2821 gattccatag ctgggatatc tcgactataa gcacttcgag aagcaaatcc ccattcctta
     2881 gcaattccgt ccatttgaga tgaagcattt aaactcattt caaccagtat aaataaagag
     2941 attagaatgg caaatagatt cacagatata aactttttga taactgcaag gagtaaaaga
     3001 gaatagacaa ccaaaaattc aagagtaagc agaatattca aatctgttaa aaaagaataa
     3061 tgcgatttta gatagatggt agctaaaaat cctgttacta caagaaaaag cgaaactaaa
     3121 aaattccaga ctttaagttc tttcagacgc tttaagactt ctgctgctgt gtaaattaac
     3181 aaggtagaga aaatccaagc atagcgatgt aaaaacatgt ttggagtatg catgccttgc
     3241 caaaataagt caagagcttc tatgtaaaag cttgcaatta gaaatgcaaa gaatattata
     3301 tatatgagtt tcacgtgaaa cctaatagat ttcagcgtaa aaaataaaat ggtcaaaata
     3361 aagggaagta gtccaacaaa aatcattggg atggccccat actttgttgt gtcaaaggaa
     3421 ccaatgaatt gcttagcaaa gagatcaaga taccagctac tttcagtttg aaactttgta
     3481 acttcagtca atttttcccc atgtgtctgt aaatcaaaca gagtgggaag agtcataatc
     3541 aaactagcca taccagctaa aaaggagata actatgaaat caagaacaga tgattttcga
     3601 gtcttaaagt cccacgaaat ttgacagaga taccagaaaa taagaaacaa tactgtcata
     3661 tatccaaaat aataattttg aataaataag attgacagac ttgtaaagta caataggagt
     3721 ttcttttcag ttatcagtag atgtaaacca gttataatta aaggaatcaa gataaaaaca
     3781 tctagccagg tttttatctc taattgactg acagtgaaac ccatcagagc ataggaagta
     3841 gataaggcta gttttaaagt ctgagggata gattgaaaca atttattcaa actaaaaaag
     3901 gttgacagac caatcaatcc aaattttaag agagtggtca gatagacagc atctggcata
     3961 ttcgttagat caaaaaagta aactagaggc gcgagaaaac tacccaagta ataactagat
     4021 agggcataga agtttagccc tagaccactt gtaaaggtgt aaaacagact actatttcca
     4081 tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt gatgaaagcc atctcctaat
     4141 agaggagagt tgtcgctatt ccagtagata ccttgagata gatatactcc agacataatc
     4201 actacaggaa tgatgaaaga aataaaatag gttcgatatg tttttaaaaa tgatttcatg
     4261 ttacctcgta gaatgataga aaactcagtt ggttaaccca actgagtttt gaagttttat
     4321 ttagtctttc caaagttctt taacttttgc ttgtacttct gcattttcta ggaattcatc
     4381 gtaggtttca tcgatacggt caatgacgcc atttttagat aagacaatga tatggttagc
     4441 caaagtttga ataaattcgt ggtcatgact agcaaagatg attgattctt taaagttttt
     4501 caatccatca ttcaagcttg agatagattc caagtccaag tgatttgttg gatcatcaag
     4561 tacaaggaca tttgatttta agagcatgag ttttgaaagc atgacacgaa ctttttctcc
     4621 ccctgacaag acatttacag gtttgttaac ttcatctcca gagaagagca tacggccgag
     4681 gaaaccacgt aggaaagtat tgtcatcttc ttctttactt gcgaattgac gcaaccagtc
     4741 aagaattgat tctcctcctg caaaatcagc tgagttatct tttggtaggt aagattgact
     4801 agttgtaact ccccacttga cagttccttc atagtcaata tctcccatga ttgcacgaat
     4861 taatgcagtc gtttgaatat cattttgtcc aataagtgct gtcttatcat ctggacgcaa
     4921 gatgaaacta atattatcca agatagtttc accatcaatc tttacagtta gattttctac
     4981 tgtcaagaga tcattaccaa tctcacgttc cgctttaaag ttgataaatg gatatttacg
     5041 actagatggt acaatctctt ctagctcaat cttatcaagc attttcttac gtgatgttgc
     5101 ctgccttgac ttagaagcat tggcagagaa acgagcaacg aattcttgca attgtttaat
     5161 tttttcttct gctttagcat tacggtctgc tagcaattta gcagcaagct cagaagattc
     5221 cttccagaag tcgtagtttc cgacatagag tttgattttt ccaaagtcaa ggtcggccat
     5281 gtgagtacaa actttgttta agaagtgacg gtcgtgggat actacgataa ctgtgttatc
     5341 aaagtcaatt aagaagtctt ctaaccaagt aatcgattgg atatccaaac cgttagtagg
     5401 ctcgtccaag agaagaacat ctggtttacc aaaaagtgct ttggcgagga gaacctttac
     5461 tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt aattcttctg gaatgtttag
     5521 gttttgaagt agttgagagg cttcactctc tgcttcccaa cctccaagtt cggcaaactc
     5581 tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag aaatcttcct tcatgtagat
     5641 agcatctttc tctttcatga tgctataaag tttttcattt cccatgataa cgacatcaat
     5701 ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga acagagagac gttcatctgg
     5761 accaagagag atgtgaccag tagtaggttc gatatctcca gctaaaattt ttaaaaaggt
     5821 tgattttccg gcaccattag caccgattaa tccgtaagta tttccttctg taaatttgat
     5881 attgacatca tcaaaaagtt tgcgatcact aaaacgtagt gaaacatcag atactgtaag
     5941 caatattttt ctcctatatg tgtaatatat ttattctact agaaaataca gaaatattca
     6001 aatttttatt tatcaatttt gtgtaaatta tatttacagt atcctttaca caaatctgta
     6061 aaaagcaagg ctgatttatt ttgataaatt acggttattt cattaaaaaa atgctataat
     6121 tgaaaggact atatcgaagg agaacaaaat gactaaaccc attattttaa caggagaccg
     6181 tccaacagga aaattgcata ttggacatta tgttggaagt ctcaaaaatc gagtattatt
     6241 acaggaagag gataagtatg atatgtttgt gttcttggct gaccaacaag ccttgacaga
     6301 tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga aatgtggctt tggattatct
     6361 tgcagttgga ttggatccaa ataagtcaac tatttttatt caaagccaga ttccagagtt
     6421 ggctgagttg tctatgtatt atatgaatct agtttcgtta gcacgtttgg agcgaaatcc
     6481 aacagtcaag acagagattt ctcagaaagg atttggagaa agcattccga caggattctt
     6541 ggtctatcca atcgctcaag cagctgatat cacagctttc aaggctaatt atgttcctgt
     6601 tgggacagat cagaaaccaa tgattgagca aactcgtgaa attgttcgtt cttttaacaa
     6661 tgcatataac tgtgatgtct tggtagagcc ggaaggtatt tatccagaaa atgagagagc
     6721 agggcgtttg cctggtttag atggaaatgc taaaatgtct aaatcactaa ataatggtat
     6781 ttatttagct gatgatgcgg atactttgcg taaaaaagta atgagtatgt atacagatcc
     6841 agatcatatc cgcgttgagg atccaggtaa gattgaggga aatatggttt tccattatct
     6901 agatgttttt ggtcgtccag aagatgctca agaaattgct gatatgaaag aacgttatca
     6961 acgaggtggt cttggtgatg tgaagaccaa gcgttatcta cttgaaatat tagaacgtga
     7021 actgggtcct attcgtgagc gccgtattga atttactaag gatatgggag aagtttataa
     7081 tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg ggtcaaaccc tatctgaggt
     7141 aaaaggggca atgggacttc attactttaa ctaagtttat tttacaagaa actatctttt
     7201 ctatctcgaa gaaaagatag ttttttattt acccctgtaa catttgacaa atttttatag
     7261 aatggtatga tagatacaat ataaaaagag tcaagctcaa aaagaaagaa aagaggaaac
     7321 ttcgaatgtc taattgggac actaaatttt tgaaaaaagg ttttaccttt gatgatgtat
     7381 tgcttattcc agctgaaagt catgtgttgc ctaacgatgc agatttaaca actaaattgg
     7441 cagataattt gactttaaat atcccaatta ttaccgctgc catggacaca gttacagaga
     7501 gtcaaatggc cattgctatt gctcgtgcag gcggtctcgg agttatccat aaaaacatgt
     7561 caattgctca acaagcagac gaggttcgta aggtaaaacg ttctgaaaat ggagttatta
     7621 ttgatccgtt cttcttgacg cctgaacata caattgctga agcagatgag cttatgggtc
     7681 gttaccgcat cagtggtgtt ccagttgttg aaacacttga aaatcgtaaa ttggttggta
     7741 ttttgacaaa ccgagatctt cgttttattt cagattataa tcaaccaatt tcaaaccata
     7801 tgactagtga aaatcttgtt actgctcctg tgggtacgga tcttgcaacg gctgagagta
     7861 ttcttcaaga gcatcgtatt gaaaaacttc cgttggtcga tgaagaaggc agtctttctg
     7921 gtttgatcac tatcaaagat attgaaaaag ttattgagtt tccaaatgcg gctaaagatg
     7981 agtttggtcg tcttctagtt gcaggtgcag taggtgttac ttcagataca tttgaacgtg
     8041 cagaggctct ttttgaggca ggagcggatg cgattgttat tgatactgca catggtcatt
     8101 ctgcaggtgt cttgcgtaaa attgccgaga ttcgtgctca tttcccagat cggactttga
     8161 ttgctggaaa tattgctact gctgaaggtg cacgtgccct ttatgaagcg ggtgtagacg
     8221 ttgttaaggt tggtattgga ccaggttcta tctgtactac tcgtgtgatt gctggtgttg
     8281 gtgttccgca agtaacagct atctacgatg ctgcagctgt tgcgcgcgaa tatggtaaaa
     8341 cgattattgc tgacggtggg atcaagtatt ctggagatat tgtaaaagca cttgctgcag
     8401 gtggaaatgc tgttatgctt ggatctatgt ttgctggaac tgatgaagct ccaggcgaaa
     8461 ctgaaatctt ccaaggacgt aaattcaaga cttaccgtgg tatgggatca attgctgcta
     8521 tgaagaaagg ttcaagcgac cgttatttcc aaggttctgt caatgaagca aacaagcttg
     8581 ttccagaagg aattgaaggt cgtgttgctt ataaaggagc ggcagctgat attgttttcc
     8641 aaatgattgg tggtattcgc tctggtatgg gttactgtgg tgcagctaac cttaaagaac
     8701 tacacgataa tgctcaattt attgaaatgt ctggtgctgg tttgaaagaa agccatcctc
     8761 atgatgtgca aattactaat gaggcaccaa attattctat gtaaaaaaca atgaaaagaa
     8821 ctccagtgaa aacaggagtt cttttacaat gttgtcaatt tccatttaca gcagctttac
     8881 catcctgaat agtgaagata cttagatttt ctggcagatt ttgaagatgg tctaagcttg
     8941 ttgttgtgat aaaggtttgg attgattgag aaatcgtttc taataatttt aactgtctag
     9001 tgttgtcaag ttcactcatc acatcgtcaa gcagtaatat aggagattct gtggtaatgc
     9061 tttccattaa ttcgatttct gctaatttta tcgagaggac gagactacga tgttgacctt
     9121 ggcttccgaa actagcatcc atcccattta tataaaaaga aatgtcatct cgatgaggac
     9181 cgacaccagt attcttttta aataaatctc tggatctact tttttctaaa gcaattttga
     9241 aagattcgga taagttttgt ttgtcagtta tattgacaga agattgatag gatattgaca
     9301 actcttcgat ctgattagag agttcaaaat gtttcttacg cccaaatgat tctagttttt
     9361 ttatgaaatc taagcggtga ttcattacac gacatccata atcaactagc tgatcatcta
     9421 acacagaaag gaatgtttca tctatttttt gagctgattt taggtaagtg tttctttgct
     9481 ttaggatgtg gttataattg gttaagtcag ataaatagat tggcttaatt tgcccaagtt
     9541 ccatatcaat gaattttcgt cgaatcgaag gtgctccttt aattagttgt aaatcttcag
     9601 gagcaaataa gacaacattc atgtgtccta cataatctga aaggcgtgcc tgttttaagt
     9661 gattaacttt tgttacacgg cctttttgtg ttagttcgat ttctagggga atggatccag
     9721 tttttttctg aacgagacct gaaagatgaa gttgttcctc atcaaaatga atgagatttt
     9781 tatctgttcg agttcgatga ctacgcgtta aggctaaaaa atagatagcc tctaacatgt
     9841 ttgtttttcc ttgtgcatta cgtcccaaaa agacattcaa tttaggatta aagtctattt
     9901 tcgtttcttt gtaattacga aaagtcttga gagatagatg ttgtagccac atgattatct
     9961 accagggaat cgtggagctt gttggctttt aggtgatgaa gcaggtttcg atttgtcttt
    10021 tttaactccc ttattcatct ttttgacaag tttagcgatc cgttcttttt caactttatc
    10081 agcttggtat tcatcttgtt cttcagaagt aggttgtgtc aacaagatgt caatgttcat
    10141 gtcagggatg tcaactttat cgccaatacg aagttttttt ccacgacaac tctctaattc
    10201 cctattaaag taaacagaat gttcagagag aaatgattta atagctcctc cgctatgtgt
    10261 aattccaagt tctttgagta gtgcttggag ggtaataaat tcttcaaata atttgtattc
    10321 cataattcac ctcaatcttt ggtattatac catattttcc taaaaatagt gagagagaca
    10381 gctagaaatg taagcgattt ttatttcaaa agtgttacag ggaacaagaa aatttcaggt
    10441 tttcgtgata taatagaagt ctgtatataa ggaggtaaat catggagtta gtgcatggaa
    10501 tttcaacaca ttttatccaa tcaaaaaagt ttaaaacaaa caaaattacc gtgcgtttta
    10561 ccgctccatt atcccttgat acgattgcag gtcacatgtt gagtgcaagt atgttagaga
    10621 ctgctaatca gatgtacccc acttctcaag atttgaggag acacttggcc agtctatacg
    10681 gtacagatat gtcaaccaat tgtttcagaa gagggcaaag ccacattata gaattgacat
    10741 ttacctatgt tcgtgatgag tttttaagta ggaaaaacgt gctaacctct cagattttgg
    10801 aacttgtaaa agaaactctt ttttcacccg cagtagttga taatgggttt gatccggcct
    10861 tatttgaaat tgagaaaaaa caattgctag caagtttagc agctgatatg gatgattctt
    10921 tttattttgc acataaagaa ttggataaat tgttttttca tgatgaacgt cttcaattgg
    10981 aatatagtga tttacgaaat cgtattttag ctgaaactcc acaaagttct tattcttgtt
    11041 tccaagaatt tttagccaat gatcgaatag atttcttttt cctaggtgat tttaatgagg
    11101 ttgaaattca aaatgtatta gaatcatttg gctttaaagg tcgaaaagga gatgtgaagg
    11161 ttcagtattg tcaaccttat tctaatatcc ttcaggaagg tatggttcgg aaaaatgtgg
    11221 gacaatccat tttggaatta ggttatcatt accgttctaa atatggtgat gaacaacatt
    11281 tacccatgat tgtaatgaat ggtttacttg gtggatttgc tcactctaag ctctttacaa
    11341 atgtccgtga aaatgctgga ttagcttata ccatttcaag tgagcttgat ttatttagtg
    11401 gattcttgag gatgtatgct ggtatcaatc gagaaaatcg taaccaggct cgtaaaatga
    11461 tgaataatca actgcttgat ttaaaaaaag gttattttac agagtttgag ttaaatcaga
    11521 ccaaggaaat gattcgttgg tcgttgttac tttctcaaga taatcaatct tcattgattg
    11581 aacgtgctta tcaaaatgcc ttatttggaa aatcttcagc agactttaaa ggttggattg
    11641 caaaactcga gcaagttgac aaagatgcta tttgtagagc agctaataat gtgaaactac
    11701 aggcgattta ctttatggaa ggaatagaat gacaaaggtt gtttttgaaa aaaaatacta
    11761 tccagctgta aaagaaatga tttatcgaac tcgtttggcc aacggattga cagttgctct
    11821 tttgcctaaa aaggaattta aagaggttta cgggagtgtc actgtacagt ttggttcggt
    11881 agatacgttt gtcacagaag ttgacggaga tgtaaaacaa tatcctgcag gaattgctca
    11941 ttttcttgaa cataaattat ttgagagaga agattctagt gatttgatgt cggcttttac
    12001 gagtctaggt gcagatagta atgcctttac aagctttaca aaaacaaact atcttttttc
    12061 agcaacggat tattttttag aaaatttata tttacttgat gaattggtaa catcagcaca
    12121 ctttactgaa gcttccattc tgacagagca ggatattatt cagcaagaac gagaaatgta
    12181 ccaagatgat ccagattcgt gtttattctt ttcaacttta gcgaatttgt atcctggtac
    12241 acctttagca actgatatag ttggaagtga ggagtccatt tcccaaatca atctaactaa
    12301 tttgcaagaa aattttacaa agttttacaa acctgtaaac atgtctctgt ttttagttgg
    12361 taattttgat gtggagcgag tacaggacta ttttgaaagc aaagaactga aagattcaga
    12421 ttttcaggaa gtagcaagag aaaagttgtt tttacagcct gtaaagccaa cagatagtat
    12481 gagaatggaa gtatcttctc ccaaactagc gattggagtt agaggtaagc gagaagtttc
    12541 tgaagcggat tgctatcgac atcatatttt attaaaatta ttgtttgcaa tgatgtttgg
    12601 ttggacttcg gatcgttttc aaaaatgtta tgaatcaggt aaaattgatg cgtccttatc
    12661 tctggaagtt gaaataacaa gtcgctttca ttttgtcatg ttgacaatgg atacgaaaga
    12721 gccagttgct ttgtctcatc aatttaggaa ggctattcgt aattttacaa aggatttaga
    12781 tattacagag gaacatttag atattatcaa aagagagatg tttggcgaat ttttcagtag
    12841 catgaactct cttgaattta ttgcaacgca atatgatgct tttgaaaatg gtgagacaat
    12901 ttttgatttg ccgaaaattt tacaggaaat tactttagag gatgtccttg atgctggaca
    12961 tcatttaata gatgatggtg acatagttga ttttacaata ttcccatcgt agtaacctat
    13021 cataatagac actagaaaga agggatgaca agtatgagaa aaaaaacaat tggagaggtt
    13081 ttacgattag ctagaatcaa tcagggattg agtttagatg aattgcagaa aaagacagaa
    13141 atccagttag atatgttgga agcaatggaa gcagacgatt tcgatcaact tccaagtcct
    13201 ttttacacgc gttctttctt gaaaaaatat gcatgggctg ttgagttaga tgaccaaatt
    13261 gttttggatg cttatgattc tgggagtatg attacttatg aggaagtaga tgttgatgaa
    13321 gatgagttga caggtcgtag acgttcaagt aagaaaaaga agaaaaaaac atcattttta
    13381 cctttatttt attttatcct ttttgcttta tcgattttaa tttttgtgac ttattatgtt
    13441 tggaactata ttcaaactca accagaggag ccttctcttt ctaattacag tgtggttcaa
    13501 tcaacaagtt caactagctc tgttccccac tcctcaagta gtagttcttc tagtatagaa
    13561 tcagctataa gtgtatcagg cgaaggaaat catgtagaaa tcgcttataa gacaagtaag
    13621 gaaacagtta aattgcaatt ggcagtttca gatgttacaa gttgggtcag tgtttcagaa
    13681 agcgaacttg agggcggtgt aaccttatcg ccaaagaaga aaagtgcaga agcaacagtt
    13741 gcaactaaaa gtcctgtaac aattacgtta ggtgttgtaa aaggtgttga tttgacagta
    13801 gataatcaga ctgttgattt atcgaaatta acagctcaga ctggacaaat cactgtaacc
    13861 tttactaaaa attaaggaaa aacgaatgaa aaaagaacaa attcccaatc tcttaacaat
    13921 aggtcgaatt ctctttatac ctatttttat ctttatttta acgataggaa attcgataga
    13981 gagtcatata gttgcagcta ttatctttgc tgttgccagt attaccgact atttagatgg
    14041 atatttagct cgtaaatgga atgtggtcag taattttggt aaatttgcag atcctatggc
    14101 ggataagtta ctagttatgt cggcttttat tatgttgatt gagttaggta tggctccggc
    14161 ttggattgtt gcagtgatta tctgtcgtga gttagctgtg acaggtttaa ggcttttatt
    14221 ggttgaaact ggtggaacaa ttttagcagc agcaatgcct ggaaaaatta aaacttttag
    14281 tcagatgttt gccattattt tcttgctatt acattggact ttgcttggtc aagttctact
    14341 ttatgtagcc ttatttttca ctatctactc tggctatgac tatttcaagg gtagtgccta
    14401 tgtatttaaa gggacatttg gttcgaaatg aaatcaataa ttgatgtaaa aaatctttct
    14461 tttcgctata aagaaaatca gaactactac gatgtgaagg atattacgtt tcacgtgaaa
    14521 cgtagagaat ggctttcgat tgtagggcat aatggtagtg gtaaatcaac gacggttcga
    14581 ttaattgatg gcttactgga agcagaatcc ggagagattg taattgatgg ccaacgtttg
    14641 actgaggaaa atgtttggaa tatacgtcgt caaatcggta tggtttttca aaatccagac
    14701 aatcaatttg ttggagcgac tgttgaagat gatgttgctt ttggtttgga aaatcaggga
    14761 ctttctcgtc aagaaatgaa aaagagagtg gaagaagctc tggctttagt tggcatgttg
    14821 gactttaaaa agagagagcc agcgcgtcta tcaggtggcc aaaagcaacg tgtggccatt
    14881 gcaggtgttg tagccctaag accagctatt ttaatcttag atgaagcaac gagtatgttg
    14941 gatcctgagg ggcgtagaga acttattggg acagtaaaag gaattcgaaa agactatgat
    15001 atgacggtca tttctattac ccatgatttg gaagaagtcg ccatgagtga tcgcgtattg
    15061 gtcatgaaaa aaggggaaat tgaatcaact agtagtccaa gggagctttt ctctcgaaat
    15121 gatttagatc aaattggatt agacgatcct tttgctaatc aattaaaaaa atctttgagc
    15181 cagaatggct atgatttacc tgaaaattat ttgacagaaa gtgagctaga ggataagcta
    15241 tgggaattgc tctagaaaat gtgaatttta catatcaaga aggtactccc ttagcttcag
    15301 cagctttgtc ggatgtttct ttgacgattg aagatggctc ttatacagct ttaattgggc
    15361 acacaggtag tggtaaatca actattttac aactcttaaa tggtttattg gtgccaagtc
    15421 aagggagtgt gagggttttt gataccttaa tcacctcgac ttctaaaaat aaagatattc
    15481 gtcaaattag aaaacaggtt ggcttggtat ttcagtttgc tgaaaatcag atttttgaag
    15541 aaacggtttt gaaggacgtt gcttttggac cgcaaaattt tggagtttct gaagaagatg
    15601 ctgtgaagac tgcgcgtgag aaactggctc tggttggaat tgatgaatca ctttttgatc
    15661 gtagtccgtt tgagctgtca gggggacaaa tgagacgtgt tgccattgca ggcatacttg
    15721 ccatggagcc agctatatta gtcttagatg agccaacagc tggtctagat cctctaggga
    15781 gaaaagagtt gatgaccctg ttcaaaaaac tccaccagtc agggatgacc atcgtcttgg
    15841 taacgcattt gatggatgat gttgctgaat atgcgaatca agtctatgta atggaaaagg
    15901 gacgtttagt aaaggggggc aaaccaagtg atgtctttca agacgttgtt tttatggaag
    15961 aagttcagtt gggagtacct aaaattacgg ccttttgtaa acgattggct gatagaggcg
    16021 tgtcatttaa acgattaccg attaagatag aggagttcaa ggagtcgcta aatggatagt
    16081 atgattttag ggcgttatat cccaggggat tcgattgttc accgattgga tccacgtagc
    16141 aaattgcttg ctatgatgct actgattttg attgtgtttt gggctaataa ccccttgacg
    16201 aatctgattc tttttatagc gacagggata tttattgcct tgtcaggagt atctctttca
    16261 ttttttattc agggcttgaa gtctatgttt ttcttgattg ccttcacaac tatttttcaa
    16321 ctatttttca tttctaatgg gaatgtttta tttgagtttt cgtttgtgag aatcacggat
    16381 tatgctttgc aacaagctgg gattattttt tgtcgctttg tattgattat tttcttttca
    16441 actttgttaa ccttaacaac catgccttta agtttggcat cagctgtcga agctttatta
    16501 gcacctttaa agcgtgtgaa agttccagtt catgaaattg gattgatgct gtctatgagt
    16561 ttgcgttttg tcccaacctt gatggatgat acgactcgga ttatgaatgc acagaaagct
    16621 cgtggagtgg attttggaga aggaagcatc gttcaaaaag taaaggcgat gattcccatt
    16681 ttgattcctc tttttgcgac aagtttaaaa cgtgcagatt ccttggctat cgctatggaa
    16741 gcgcgtggct atcagggtgg aaaaggcaga agtcaatacc gacaattgaa atggactctg
    16801 aaggatacgc tgaccattct tgttattctc gtacttggtt gttgtttatt tttcttaaaa
    16861 tcttagtagc tttcaggaat tgataaaaag ttactgtaac agtttttgat ataaaggtag
    16921 gaatatgaac cgttttaaaa aatcaaaata tgtcattatt gtttttgtca ctgttctgct
    16981 tgtgtcagct ctcttagcga cgacttattc aagtacaatt gtgacaaaat taggagatgg
    17041 aatctcattg gttgatagag ttgtacaaaa accttttcag tggtttgatt ctgtcaaatc
    17101 agatttggct catttgacac gaacatataa tgaaaatgaa agtttgaaga aacagcttta
    17161 ccaattagaa gttaaatcaa atgaggtgga aagtttaaag acagaaaatg aacaactgcg
    17221 ccaattgctt gatatgaagt ctaaattgca agccacaaag actttagcag cagatgttat
    17281 tatgcgttct ccggtatctt ggaagcagga gttgacctta gatgcaggta gatcaaaagg
    17341 tgcttctgag aacatgttag ctattgcaaa tggtggcttg attgggagtg tttcaaaagt
    17401 agaggagaac tctactatag tcaaccttct gacaaatacg gaaaatgctg ataagatttc
    17461 tgttaaaatt caacatggct ctactacaat ttatggaatt attattggct atgacaagga
    17521 aaatgacgtt cttaaaatta gccaattaaa tagtaatagc gatattagtg cgggagataa
    17581 ggtgactact ggtggattag gaaactttaa cgttgctgat attcctgttg gtgaagtggt
    17641 tgccacaacg catagtacag actatttgac acgagaagta actgttaaat tgagtgcaga
    17701 tactcataat gtagatgtga tagaattagt ggggaattca taatgagaca gttgaagcga
    17761 gttggagtat ttttattgct tcctttcttt gttctaattg acgcccatat tagccagctt
    17821 ctgggctcat ttttccccca tgtacatttg gctagtcatt ttctttttct atttctctta
    17881 tttgagacga tagaagtatc agagtatctc tacctagtct attgttttgt tataggcttg
    17941 gtttatgatg tttacttttt ccatctaata gggattacaa ctctcttatt tatcttattg
    18001 ggagccttcc ttcataaatt gaatagtgtt attttgttga atcgttggac aagaatgcta
    18061 gctatgattg tgctgacatt cctgtttgaa atgggtagtt atcttttggc ttttatggta
    18121 gggttgacag tagatagcat gtcgattttt atagtctata gcttggtacc gacgatgatt
    18181 ttaaattttt tatggattac tgtttttcaa tttatttttg aaaaatatta tctataagaa
    18241 cgacatataa atgtaacaaa ggcgtaatat ttattaggcc tttttttggt atactagtat
    18301 tgtctttaaa agaaggagta tctacgtaat atgaagaaaa aaatcttagc gtcactttta
    18361 ttaagtacag taatggtttc tcaagtagct gttttaacaa ctgcgcatgc agaaacgact
    18421 gatgacaaaa ttgctgctca agataataaa attagtaact taacagcaca acaacaagaa
    18481 gcccaaaaac aagttgacca aattcaggag caagtatcag ctattcaagc tgagcagtct
    18541 aacttgcaag ctgaaaatga tagattacaa gcagaatcta agaaactcga gggtgagatt
    18601 acagaacttt ctaaaaacat tgtttctcgt aaccaatcgt tggaaaaaca agctcgtagt
    18661 gctcaaacaa atggagccgt aactagctat atcaatacca ttgtaaactc aaaatcaatt
    18721 acagaagcta tttcacgtgt tgctgcaatg agtgaaatcg tatctgcaaa caacaaaatg
    18781 ttagaacaac aaaaggcaga taaaaaagct atttctgaaa aacaagtagc aaataatgat
    18841 gctatcaata ctgtaattgc taatcaacaa aaattggctg atgatgctca agcgttgact
    18901 acgaaacagg cagaactaaa agctgctgaa ttaagtcttg ctgctgagaa agcgacagct
    18961 gaaggggaaa aagcaagtct attagagcaa aaagcagcag ctgaggcaga ggctcgtgca
    19021 gctgcggtag cagaagcagc ttataaagaa aaacgagcta gccaacaaca atcagtactt
    19081 gcttcagcaa acactaactt aacagctcaa gtgcaagcag tatctgaatc tgcagcagca
    19141 cctgtccgtg caaaagttcg tccaacatac agtacaaacg cttcaagtta tccaattgga
    19201 gaatgtacat ggggagtaaa aacattggca ccttgggctg gagactactg gggtaatgga
    19261 gcacagtggg ctacaagtgc agcagcagca ggtttccgta caggttcaac acctcaagtt
    19321 ggagcaattg catgttggaa tgatggtgga tatggtcacg tagcggttgt tacagctgtt
    19381 gaatcaacaa cacgtatcca agtatcagaa tcaaattatg caggtaatcg tacaattgga
    19441 aatcaccgtg gatggttcaa tccaacaaca acttctgaag gttttgttac atatatttat
    19501 gcagattaat ttacagaggg actcgaatag agccctcttt tcaggtttta ccgtgacaat
    19561 ccctattaaa aattatatca aaatagcttg aaaatattgg aaaagtatgg taaaatgaaa
    19621 attgtcgtgt gaacgataat actcattctt gatgaattgt gaagcagttg cccttgggtc
    19681 gttttgcgag ttgaagtcaa gaagaggaaa aaaacaaaaa ggagaaatac tcatggcagt
    19741 aatttcaatg aaacaacttc ttgaggctgg tgtacacttt ggtcaccaaa ctcgtcgctg
    19801 gaatcctaag atggctaagt acatctttac tgaacgtaac ggaatccacg ttatcgactt
    19861 gcaacaaact gtaaaatacg ctgaccaagc atacgacttc atgcgtgatg cagcagctaa
    19921 cgatgcagtt gtattgttcg ttggtactaa gaaacaagca gctgatgcag ttgctgaaga
    19981 agcagtacgt tcaggtcaat acttcatcaa ccaccgttgg ttgggtggaa ctcttacaaa
    20041 ctggggaaca atccaaaaac gtatcgctcg tttgaaagaa attaaacgta tggaagaaga
    20101 tggaactttc gaagttcttc ctaagaaaga agttgcactt cttaacaaac aacgtgcgcg
    20161 tcttgaaaaa ttcttgggcg gtatcgaaga tatgcctcgt atcccagatg tgatgtacgt
    20221 agttgaccca cataaagagc aaatcgctgt taaagaagct aaaaaattgg gaatcccagt
    20281 tgtagcgatg gttgacacca atactgatcc agatgatatc gatgtaatca tcccagctaa
    20341 cgatgacgct atccgtgctg ttaaattgat cacagctaaa ttggctgacg ctattatcga
    20401 aggacgtcaa ggtgaggatg cagtagcagt tgaagcagaa tttgcagctt cagaaactca
    20461 agcagattca attgaagaaa tcgttgaagt tgtagaaggt gacaacgctt aatttataca
    20521 aatagtaatt acctaggagg gcggggctta gcccggctct cctattttca aaaaatatag
    20581 gagaattaaa atggcagaaa ttacagctaa acttgtaaaa gagttgcgtg aaaaatctgg
    20641 tgccggtgtt atggacgcta aaaaagcgct tgtagaaaca gacggtgaca tcgaaaaagc
    20701 gattgaattg cttcgtgaaa aaggtatggc taaggcagct aagaaagctg accgtgttgc
    20761 tgcagaaggt ttgactggtg tttatgttaa cggtaatgtt gcagcagtta ttgaagtaaa
    20821 cgctgaaact gacttcgttg caaaaaacgc tcaattcgtt gaattggtaa atactacagc
    20881 taaagtcatt gctgaaggaa aacctgctaa caatgaagaa gctcttgctt tgataatgcc
    20941 ttcaggtgaa actcttgaag ctgcatacgt atctgcaaca gcaactatcg gagagaaaat
    21001 ctcattccgt cgctttgcat tgattgaaaa aacagacgca caacactttg gagcatacca
    21061 acataacggt ggacgtatcg gtgttatttc agttgttgaa ggtggagacg aagcacttgc
    21121 taaacaattg tcaatgcaca tcgcagcgat gaaaccaaca gttctttctt acaaagaatt
    21181 ggatgagcaa ttcgttaaag atgagttggc acaattgaat cacgttatcg accaagacaa
    21241 cgaaagccgt gcaatggtca ataaaccagc tcttccacac ttgaagtatg gatcaaaagc
    21301 tcaattaact gatgatgtta ttgctcaagc tgaagctgac atcaaagctg aattggctgc
    21361 agaaggcaaa ccagaaaaaa tctgggacaa aattattcca ggtaaaatgg atcgcttcat
    21421 gcttgataac actaaagttg accaagctta cacacttctt gcacaagttt acatcatgga
    21481 tgacagcaag acagttgaag cataccttga atcagttaac gcttcggtag ttgagtttgc
    21541 tcgctttgaa gttggtgaag gtatcgagaa agctgcaaac gacttcgaag ctgaagttgc
    21601 agctacaatg gcagcagcct tgaataacta ataatagaaa ggaagcaaat tgcttccttt
    21661 tttgtatagg gcttagattt ttcctgaaat tgttttttcc ccagtctcac tcgattagaa
    21721 taattaaaac aaaaagccct ataataaggg ctaagggcat ttaggagact atacttcaaa
    21781 ttcatagtaa gcgcgttcat aacaaggtat ctatcctaat tccccaactt ccagtatact
    21841 agataaaaaa taattatcgg aggaaaggta ttgtactatg ataattcaac taagtgattt
    21901 aggtcaagtt caccttgttt gtagcaagat agatatgagg cagggaatag actcattatc
    21961 ctatgtagtt aaaacccact ttgaattgga tcctttctcc ggtcaagtct ttctcttttg
    22021 tggtggacgt aaagaccgct ttaaagccct ttactgggat ggtcaaggat tttggctact
    22081 atataaacgc tttgagaacg tcagactgac ttggcccagt acagaaaagg atgtcaaagc
    22141 tctcacacct gaacaagtag actggcttat gaagggcttt tctatcactc caaaaatata
    22201 gtagattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
    22261 ctgtcctgat cgatttgtcc tgttcttatt tcattttact ataaatccat cagaaagtcg
    22321 tgatttctat tgaaatgagg actttctttt tatactcatc tgttttcaaa aagcattcta
    22381 gtccatctcc gattaacgat ggactttatc acctccttct ccagtccttg tataacatct
    22441 tggagttgat tcatgacatc ttccaaagtt tgaaaggttt tattcttaaa tccacgttta
    22501 cgaatctctt tccacacttg ttcaatgggg ttcatctctg gtgtgtatgg aggaataaat
    22561 gcaaagccaa tattagtcgg aatctttaag gtacttgatt tatgccatat agcattgtcc
    22621 ataacgagta aaagataatc atctggataa gcttgtgaaa tctcctattc ctaaagcccc
    22681 tttagcgcat aactttggct cagcttctat tatcgctcac accatccatc agaagtttaa
    22741 tctgaaggta cccaattatc gccaagaaga agattgggct aggatgggtt taccaatcac
    22801 acgtaaggaa atctctaatc ggcatatcaa gacgagtcaa tactatttgg agccccttta
    22861 taacctcttg cgagagagac tattgactca gcccttactt catgcggatg aaacttctta
    22921 tagggtgcta gagagtgata gtcagctgac ttactattgg acttttttgt ctgggaaagc
    22981 tgagaaacaa gggattacgc tttaccatca tgtcctaatc gatttgttca tatcttattt
    23041 caatccatta taaatagtga gaaatatcta tcctatcttc tagaatgtct tccaaacgtg
    23101 gaaactctcg taaacaaaga ggttttagag gcctatttac catggactaa agttgtacaa
    23161 gaaaagtgca aataagaaat ctccagatta ggaactatcc gtgagttctc tagtctggag
    23221 atttttcaat agacttcgtt attggacggt tacaattcat aaagtgctgt agagagataa
    23281 cgttcaccgt tatctggtgc tagggcaagg acttttttac ctgtacctaa ttttttggca
    23341 acctcgatgg ctccgtagat agctgcagct gaggaaatcc ctacaaggaa gccttctttt
    23401 ccaccaattt cacgtccgag tgcaagagcg tcatctgatg ttacacgaac gataccatca
    23461 taggctttag tatcaagtgt atcaggaata aatccagctg agataccttg aattttgtga
    23521 ggaccaggtt tttcaccaga tagaatagca gattcatctg cttctactgc aaaaacttga
    23581 atgttagaat tttctgattt gagtgcatga gaaacaccag aaatcgttcc accagtacct
    23641 actccagcaa caaaggcatc taatccatct ttaccgaaag cagctagtat ctcagctcct
    23701 gttgttcttt cgtgtacttc tggattagct ggattgtcaa attgaagagg aaggaaacca
    23761 tcacgttcag cagcgatttc ttgagcctta gcaatagcac ctttcattcc ctcgctacca
    23821 ggagttagga cgagttcagc accataagct tggataattt tacgtcgttc tacactcata
    23881 gtttcaggca taacgatgac gactttatac cctttagcag cacctaccca tgaaagtcca
    23941 ataccggtgt ttccacttgt tgcttcaaca atagtagaac caagtttcag aataccatct
    24001 tgttcagctt tttcaatcat gctaagggca atacggtctt ttacagatga accaggatta
    24061 aatgcttcaa gctttatata gacgtctgca gcaccttctg gcacgatgtt gttaagttta
    24121 acaatcggtg tttgaccgat taattcagta atgttgttat aaatagacat atgaatacct
    24181 ctttgtataa gatatcttta gtataacgct atccatacca tttgtaaaat atagaaatcc
    24241 tatcgaagcg ataggatttc tatatatcat agatcaaagt cattaatttt caagtgattg
    24301 cgttcagtta attctagtaa ataggtgtga aggaaaacga tatcaggttt cgtttgaatt
    24361 gcttcatttt ctagtatatt ttggtacgca tctttttatt ttgctcgcta aagtagttgt
    24421 gagaatgtca acgaaacgct gtattattca ttgtttacac taagtttaca ggaacttcaa
    24481 cctctcgtaa accttggtca gttaaagtga cttttccatt aaaaaactcc acaagtgcag
    24541 ctttaatagt ttctttttct tctttatcaa cataaatcat cgtatcgact tgatctgtaa
    24601 agtttgtatc cagctccatg agatcatgtt ctttaaggaa gttactgtac tcttggtact
    24661 gagcataaga catttgaata gcaatgccag cctgttcttt tatttcaata ataccaattt
    24721 ctttgacagc taaggcgaca ctgccggcgt aagcacgaat tagtcctcca gcgcctagtt
    24781 taataccacc aaagtagcgt gtcacgacca cacagacatt ggtgagattg tgattttcta
    24841 gtaccccaag catgggaaca ccagcagtac cactaggctc accatcatca cttgtacgtt
    24901 taatttcact acgttctcca ataataaagg cagagcagtt atgtgtcgct ttgtagtgtt
    24961 cttttttgat ggcagtaatg aagtcacgag cctcttcttc gctataaaca cgcttggcat
    25021 ggcagataaa gcgagatttt ttgatttctt cttggacttg gccgtcctct ttaattgtcc
    25081 taaattccat gatttaattg tactaaaaaa tcatctaaag tgctagtttt tacgaataaa
    25141 gaagtatgaa agtaaattta gattatctcg gtcgtttatt tactgagaat gaattaacag
    25201 aagaagaacg tcagttggcg gagaaacttc cagcaatgag aaaggagaag gggaaacttt
    25261 tctgtcaacg ttgtaatagc actattctag cagaatggta tttgcccatc ggtgcttact
    25321 attgtcgaga gtgcttgctg atgaagcgag tcagaagtga tcaaacttta tactattttc
    25381 cgcaggagga ttttccgaag caagatgttc tcaaatggcg cggccaatta actccttttc
    25441 aagagaaggt gtcagaggga ctgcttcaag cagtagacaa gcaaaagcca accttagttc
    25501 atgcggtaac aggagctgga aagacagaaa tgatttatca agtagtggct aaagtgatca
    25561 atgcgggtgg tgcagtgtgt ttggctagtc ctcgcataga tgtttgtttg gagctgtaca
    25621 agcgcctgca acaggatttt tcttgcggga tagctttgct acatggagaa tcggaacctt
    25681 attttcgaac accactagtt gttgcaacaa cccatcagtt attgaagttt tatcaagctt
    25741 ttgatttgct gatagtggat gaagtagatg cttttcctta tgttgataat cccacgcttt
    25801 accatgctgt caagaatagt gtaaaggaga atggattgag aatcttttta acagcgactt
    25861 cgaccaatga gttagataaa aaggtccgtt taggagaact aaaaagactg aatttaccga
    25921 gacggtttca tggaaatccg ttgattattc caaaaccaat ttggttatcg gattttaatc
    25981 gctacttaga caagaatcgt ttgtcaccaa agttaaagtc ctatattgag aagcagagaa
    26041 agacagctta tccgttactc atttttgctt cagaaattaa gaaaggggag cagttagaag
    26101 aaatcttaca ggagcaattt ccaaatgaga aaattggctt tgtatcttct gtaacagagg
    26161 atcgattaga gcaagtacaa gcttttcgag atggagaact gacaatactt atcagtacga
    26221 caatcttgga gcgtggagtt accttccctt gtgtggatgt tttcgtagta gaggccaatc
    26281 atcgtttgtt taccaagtct agtttgattc agattggtgg acgagttgga cgaagcatgg
    26341 atagaccgac aggagatttg cttttcttcc atgatgggtt aaatgcttca atcaagaagg
    26401 cgattaagga aattcagatg atgaataagg aggctggtct atgaagtgct tgttatgtgg
    26461 gcagactatg aagactgttt taacttttag tagtctctta cttctgagga atgatgactc
    26521 ttgtctttgt tcagactgtg attctacttt tgaaagaatt ggggaagaga actgtccaaa
    26581 ttgtatgaaa acagagttgt caacaaagtg tcaagattgt caactttggt gtaaagaagg
    26641 agttgaagtc agtcatagag cgatttttac ttacaatcaa gctatgaagg attttttcag
    26701 tcggtataag tttgatggag acttcctgtt aagaaaagtt ttcgcttcat ttttaagtga
    26761 ggagttgaaa aagtacaaag agtatcaatt tgttgtaatt cccctaagtc ctgatagata
    26821 tgctaataga ggatttaatc aggttgaggg cttggtagag gcagcaggct ttgagtatct
    26881 ggatttatta gagaaaagag aagagagagc cagttcttct aaaaatcgtt cagagcgctt
    26941 ggggacagaa cttcctttct ttattaaaag tggagtcact attcctaaaa aaatcctact
    27001 tatagatgat atctatacta caggagcaac tataaatcgt gttaagaaac tgttggaaga
    27061 agctggtgct aaggatgtaa aaacattttc ccttgtaaga tgaggaaaaa atttgcaaaa
    27121 atagaatgaa agcgttataa taaaatcata aaaacaaaaa tgtttagaaa gaaggtactc
    27181 atatgattaa atatagtatc cgtggtgaaa acctagaagt aacagaagca attcgtgatt
    27241 atgtagtttc taaactcgaa aagatcgaaa agtacttcca accagaacaa gagttggatg
    27301 cccgaattaa cttaaaagtt tatcgtgaaa aaacggctaa agtggaagta acgattccgc
    27361 ttggatctat tactctccgc gcagaagatg tatctcaaga tatgtatggt tcaattgacc
    27421 ttgtaactga taaaattgaa cgtcagattc gtaaaaataa aacaaaaatc gagcgtaaaa
    27481 ataaaaataa ggtagcaact ggtcaattat ttacagatgc tttggtggaa gattcaaata
    27541 ttgtccagtc taaagttgtt cgttcaaaac aaattgattt aaaaccaatg gatttggaag
    27601 aagcaattct acaaatggat ttattggggc atgatttctt tatctatgtg gatgttgaag
    27661 atcagacaac caatgtgatt tatcgtcgtg aggatggcga aattggtttg ttagaggtta
    27721 aagaatctta atttcaatat gtgtaaaggt aggtttactg aattgtaaac tacctttttc
    27781 ttataattga tagaattact gattacactt ttaaaaagtc gctttttgag gggtattatg
    27841 gtataatggg tgccaagagg ttttgaatga aaaaatttta tgtaagtcca atttttccta
    27901 ttctagtagg attgattgcg tttggagtct tatccacttt cattattttt gttaataata
    27961 atctgttgac ggttttaatt ttgtttcttt ttgtaggagg ctatgttttt ttatttaaga
    28021 aactgagagt gcattataca aggagtgatg tagaacagat acagtatgta aaccaccaag
    28081 cggaagaaag tttgacagct ctattggaac agatgcctgt aggtgttatg aaattgaatt
    28141 tatcttctgg agaggttgag tggtttaatc cctatgctga attgattttg accaaggaag
    28201 atggtgattt tgatttagaa gctgttcaaa cgattatcaa ggcttcagta ggaaatccgt
    28261 ctacttatgc caagcttggt gagaagcgtt atgctgttca tatggatgct tcttccggtg
    28321 ttttgtattt tgtagatgta tccagggaac aagccataac agatgaattg gtaacaagta
    28381 gaccagtgat tgggattgtc tctgtggata attatgatga tttggaggat gcaacttctg
    28441 agtcagatat tagtcaaatc aatagttttg tagctaattt tatatcagag ttttcagaaa
    28501 aatacatgat gttttctcgt cgggtaagta tggatcgatt ttatctattt actgactaca
    28561 cggtgcttga gggcttgatg aatgataaat tttctgttat tgatgctttc agagaagagt
    28621 cgaaacagag acagttgccc ttgaccttaa gtatgggatt ttcttatggc gatggaaatc
    28681 atgatgagat agggaaagtt gctttgctca atttgaactt ggctgaagta cgtggtggcg
    28741 accaggtggt tgttaaggaa aacgacgaaa cgaaaaatcc agtttatttt ggtggtgggt
    28801 ctgctgcttc aatcaagcgt acacggactc gtacgcgcgc tatgatgaca gctatttcag
    28861 ataagattcg gagtgtagat caggtttttg tagtcggtca caaaaattta gacatggatg
    28921 ctttgggctc tgctgtaggt atgcagttgt tcgccagcaa tgtgattgaa aatagctatg
    28981 ctctttatga tgaagaacaa atgtctccag atattgaacg agctgtttca ttcatagaaa
    29041 aagaaggagt tacgaagttg ttgtctgtta aggatgcaat ggggatggtg accaatcgtt
    29101 ctttgttgat tcttgtagac cattcaaaga cagccttaac attatcaaaa gaattttatg
    29161 atttatttac ccaaaccatt gttattgacc accatagaag ggatcaggat tttccagata
    29221 atgcggttat tacttatatc gaaagtggtg caagtagtgc cagtgagttg gtaacggaat
    29281 tgattcagtt ccagaattct aagaaaaatc gtttgagtcg tatgcaagca agtgttttga
    29341 tggctggtat gatgttggat actaaaaatt tcacctcgcg agtaactagt cggacatttg
    29401 atgttgctag ctatctcaga acgcgcggaa gtgatagtat tgctatccag gaaatcgctg
    29461 cgacagattt tgaagaatat cgtgaggtca atgaacttat tttacagggg tgtaaattag
    29521 gttcagatgt actaatagca gaggctaagg acacgaaatg ctatgataca gttgttatta
    29581 gtaaggcagc agatgccatg ttagccatgt caggtattga agcgagtttt gttcttgcga
    29641 agaatacaca aggatttatc tctatctcag ctcgaagtcg tagtaaactg aatgtacaac
    29701 ggattatgga agagttaggc ggtggaggcc actttaattt ggcagcagct caaattaaag
    29761 atgtaacctt gtcagaagca ggtgaaaaac tgacagaaat tgtattaaat gaaataaagg
    29821 aaaaggagaa agaagaatga aagtaatctt tttagcagat gttaaaggaa aaggtaaaaa
    29881 aggcgaaatt aaggaagtac caacagggta tgcgcaaaac tttcttatca aaaagaatct
    29941 agccaaagaa gcgactgctc aagctgtagg tgaacttcgt ggtaaacaaa aatcggaaga
    30001 aaaagctcac gctgagatga ttgcagaagg aaaagcaatt aaagcacaac ttgaagcaga
    30061 agaaactgtt gtagaatttg ttgaaaaagt tggtccagat ggtcgtacct ttggttctat
    30121 taccaataag aagattgcag aagaattgca aaagcaattt ggaattaaga ttgataaacg
    30181 tcatattcaa gtacaagctc cgattcgagc ggttggtttg attgatgtgc cagtgaaaat
    30241 ctatcaagat atcacaagtg taatcaatct tcgtgtgaaa gaaggataag tttacacctt
    30301 cttgacaaga ttgtaaaagg aagggaagtc tgatggcaga agtagaagag ttacgagtac
    30361 aacctcaaga tatcttagct gagcaatccg ttttaggggc tatctttatt gatgagagta
    30421 aacttgtttt tgtgcgagaa tacattgagt ctcgggactt ttttaagtat gcccatcgtt
    30481 tgattttcca agccatggtc gatttatccg atcgtggtga tgccatagat gcaacaacgg
    30541 ttcgtactat ccttgataat caaggtgatt tacagaatat tggtggcttg tcttacttgg
    30601 ttgagattgt caattctgtg ccaacttctg ctaatgcgga gtattatgct aagattgttg
    30661 cagaaaaagc aatgctacgt cgtttaattg ccaagttgac agagtctgtc aaccaagctt
    30721 acgaagcgtc acaaccagct gatgaaatta ttgctcaggc agaaaaaagg ttgattgatg
    30781 tcagtgaaaa tgcaaatcga agcgggttta agaacattcg agatgtgttg aatctcaact
    30841 ttggaaatct ggaagctcgc tcgcaacaaa cgaccgatat tacaggtatt gcgacaggtt
    30901 atcgtgattt ggatcatatg acaacaggac ttcatgagga ggagttgatt atcttagcag
    30961 ctcgtccagc agttggtaag acagcatttg ccttgaatat cgctcagaac attgggacta
    31021 agttggacaa aacggttgct attttttcac tcgaaatggg tgcggaaagc ttggtagatc
    31081 gtatgttagc tgcagaaggc ttagtggagt cacattctat ccgtacaggg caattgacag
    31141 atgaggagtg gcaaaaatat actattgctc agggtaatct agctaacgcc agtatctata
    31201 tcgatgatac gccaggtatt cggattacag agattcgttc tcgttctcgt aaattggctc
    31261 aagaaactgg aaatcttggt ttgattttga tagactattt gcaacttatc acgggaactg
    31321 gtcgagaaaa tcgtcaacaa gaagtttctg aaatttctcg tcagttgaaa atactagcca
    31381 aggaattgaa ggttccagta atcgctctga gtcagctttc tcgtggtgta gaacaacgtc
    31441 aggacaagag accggtcttg tctgatattc gtgaatctgg gtctattgag caggacgctg
    31501 atatcgtagc ttttctctat cgcgatgact actatgaacg tggtggtgaa gaagaggagg
    31561 gtatcccaaa taataaggtg gaagttatta tcgagaaaaa ccgtagtgga gctcgtggaa
    31621 cagtggaatt gattttccaa aaagaataca ataaattttc aagtatctca aagagggagg
    31681 cataagatgt cagatgcatt tacagatgta gccaagatga aaaaaatcaa agaagaaatc
    31741 aaggcacatg agggacaagt cgtagaaatg actttggaga atggtcgtaa gcgccaaaaa
    31801 aatagattgg gtaagctaat tgaagtttat ccatctctat ttattgtgga gtttggggat
    31861 gtggaaggag ataaacaagt taatgtttac gttgaatcct ttacttactc agatattctt
    31921 acagaaaaga atttgattca ttatcttgac taaagtgaga aattttctca ctttttcttt
    31981 tttctccgaa taatttaggt gaaggcaatc atcgctttat attatttttc aaggaggaag
    32041 aatgaaaatt ttaccgttta tagcaagagg aacaagttat tacttgaaga tgtcagttaa
    32101 aaagcttgtt ccttttttag tagtaggatt gatgctagca gctggtgata gtgtctatgc
    32161 ctattccaga ggaaatggat cgattgcgcg tggggatgat tatcctgctt attataaaaa
    32221 tgggagccag gagattgatc agtggcgcat gtattctcgt cagtgtactt cttttgtagc
    32281 ctttcgtttg agtaatgtca atggttttga aattccggca gcttatggaa atgcgaatga
    32341 atggggacat cgtgctcgtc gggaaggtta tcgtgtagat aatacaccga cgattggttc
    32401 cattacttgg tctactgcag gaacttatgg tcatgttgcc tgggtgtcaa atgtaatggg
    32461 agatcagatt gagattgagg aatataacta tggttataca gaatcctata ataaacgagt
    32521 tataaaagca aacacgatga caggatttat tcattttaaa gatttggatg gcggcagtgt
    32581 tgggaatagt caatcctcag cttcaacagg cggaactcat tattttaaga ccaagtctgc
    32641 tattaaaact gaacccctag ctagcggaac tgtgattgat tactattatc ctggggagaa
    32701 ggttcattat gatcagatac ttgaaaaaga cggctataag tggttgagtt atactgccta
    32761 taatggaagc tatcgttatg ttcaattgga ggctgtgaat aaaaatcctc taggtaattc
    32821 tgttctttct tcaacaggtg gaactcatta ttttaagacc aagtctgcta tcaaaactga
    32881 acccctagtt agtgcaactg tgattgatta ctattatcct ggggagaagg ttcattatga
    32941 tcagatactt gaaaaagacg gctacaagtg gttgagttat actgcctata atggaagtcg
    33001 tcgctatata cagctagagg gagtgacttc ttcacaaaat tatcagaatc aatcaggaaa
    33061 tatctctagc tatggatcca ataatagttc aactgtcggt tggaagaaaa taaatggtag
    33121 ttggtatcat ttcaaatcaa atggttctaa atcaacagga tggctgaaag acggttctag
    33181 ctggtattat ttgaaattat ctggtgaaat gcagacagga tggttaaagg agaatggctc
    33241 gtggtattat ctgggtagtt caggggcaat gaaaacaggc tggtaccagg tctctggtaa
    33301 gtggtattat tcttactctt caggcgcctt agctgttaat acgacggtgg atggctacag
    33361 agtaaacagt gatggagaac gagtatagaa aattggagta ggagaaattt cctgctcttt
    33421 tctgtaagca gtttccttga catttttctt attttgcgct atcatatata catataatat
    33481 atgggagtct gtgtacagtc tgagaggaag tgttaaactt cgaccgcacc tgatctgggt
    33541 aatgccagcg gagggaacga tacttagtct aattttgcac cttttccatg tatggtaaag
    33601 gtttttcttt tttttaaaag gaaaacgaga agaggaggtt cttatgaaag caagcattgc
    33661 cttgcaagtt ttacccctag cacaggggat tgatcggata gctgttattg atcaggtcat
    33721 tgcttatctg caaactcaag aagtgacgat ggtagtgaca ccatttgaaa cggtcttgga
    33781 aggggagttt gatgagctta tgcgcattct aaaagaagcg ctggaagtgg cagggcagga
    33841 ggcagacaat gtctttgcca atgtcaaaat aaatgtagga gagattttaa gtattgatga
    33901 gaaacttgaa aagtatactg agacgacaca ttagtctatt gggctttctc ggagtattgt
    33961 caatctggca gttagcaggt tttcttaaac ttctccccaa gtttatcctg ccgacacctc
    34021 ttgaaattct ccagcccttt gttcgtgaca gagaatttct ctggcaccat agctgggcga
    34081 ccttgagagt ggctttactg gggctgattt tgggagtttt gattgcctgt cttatggctg
    34141 tgctcatgga tagtttgact tggctcaatg acctgattta ccctatgatg gtggtcattc
    34201 agaccattcc gaccattgcc atagctccta tcctggtctt gtggctgggt tatgggattt
    34261 tgcccaagat tgtcttgatt atcttaacaa caacctttcc catcatcgtt agtattttgg
    34321 acggttttag gcattgtgac aaggatatgc tgactttgtt tagtctgatg cgagcaaacc
    34381 cttggcaaat cctgtggcat tttaaaatcc cggttagcct gccttacttt tatgcaggtc
    34441 tgagggtcag tgtctcctac gcctttatca caactgtggt atctgagtgg ttgggaggtt
    34501 ttgaaggtct tggtgtttat atgattcagt ctaaaaaact gtttcagtat gataccatgt
    34561 ttgccattat tattctggtg tcgattatca gtcttttggg tatgaagctg gtcgatatca
    34621 gtgaaaaata tgtgattaaa tggaaacgta cttaaaatag agcgtttttg aaaaagaaaa
    34681 gaggaaatca aaatgaagaa aacatggaaa gtgtttttaa cgcttgtaac agctcttgta
    34741 gctgttgtgc ttgtggcctg tggtcaagga actgcttcta aagacaacaa agaggcagaa
    34801 cttaagaagg ttgactttat cctagactgg acaccaaata ccaaccacac agggctttat
    34861 gttgccaagg aaaaaggtta tttcaaagaa gctggagtgg atgttgattt gaaattgcca
    34921 ccagaagaaa gttcttctga cttggttatc aacggaaagg caccatttgc agtgtatttc
    34981 caagactaca tggctaagaa attggaaaaa ggagcaggaa tcactgccgt tgcagctatt
    35041 gttgaacaca atacatcagg aatcatctct cgtaaatctg ataatgtaag cagtccaaaa
    35101 gacttggttg gtaagaaata tgggacatgg aatgacccaa ctgaacttgc tatgttgaaa
    35161 accttggtag aatctcaagg tggagacttt gagaaggttg aaaaagtacc aaataacgac
    35221 tcaaactcaa tcacaccgat tgccaatggc gtctttgata ctgcttggat ttactacggt
    35281 tgggatggta tccttgctaa atctcaaggt gtagatgcta acttcatgta cttgaaagac
    35341 tatgtcaagg agtttgacta ctattcacca gttatcatcg caaacaacga ctatctgaaa
    35401 gataacaaag aagaagctcg caaagtcatc caagccatca aaaaaggcta ccaatatgcc
    35461 atggaacatc cagaagaagc tgcagatatt ctcatcaaga atgcacctga actcaaggaa
    35521 aaacgtgact ttgtcatcga atctcaaaaa tacttgtcaa aagaatacgc aagcgacaag
    35581 gaaaaatggg gtcaatttga cgcagctcgc tggaatgctt tctataaatg ggataaagaa
    35641 aatggtatcc ttaaagaaga cttgacagac aaaggcttca ccaacgaatt tgtgaaataa
    35701 tgacagaaat tagactagag cacgtcagtt atgcctatgg tcaggagagg attttagagg
    35761 atatcaacct acaggtgact tcaggcgaag tggtttccat cctaggccca agtggtgttg
    35821 gaaagaccac cctctttaat ctaatcgctg ggattttaga agttcagtca gggagaattg
    35881 tccttgatgg tgaagaaaat cccaaggggc gcgtgagtta tatgttgcaa aaggatctgc
    35941 tcttggagca caagacggtg cttggaaata tcattctacc cctcttgatt caaaaggtgg
    36001 ataaggcaga aactatttcc cgagcggata aaattcttgc gaccttccag ctgacagctg
    36061 taagagacaa gtatcctcat gaacttagcg gtgggatgcg ccagcgtgta gccttactcc
    36121 ggacctacct ttttgggcat aagctctttc tcttagatga ggcctttagc gccttggatg
    36181 agatgacaaa gatggaactc cacgcttggt atcttgagat tcacaagcag ttgcagctaa
    36241 caaccctgat catcacgcat agtattgagg aggccctcaa tctcagcgac cgcatctata
    36301 tcttgaaaaa tcgccctggg cagattgttt cagaaattaa actagattgg tctgaagatg
    36361 aggacaagga agtccaaaag attgcctaca aacgtcaaat cttggcagaa ttaggcttag
    36421 ataagtagaa aaatagggag ttggtgaaga ttatccttta ccagcgccct ttttctttta
    36481 aaaatgagaa aatttcggta taatagtcaa acaaggtcaa ggtttaaaga gagaggtggg
    36541 tttgttatga gatttaaaaa tacatcggat catattgagg cctacatcaa ggcgatttta
    36601 gatcaatctg gtatcgtgga gttgcaacgg agtcagttgg cagatacctt tcaggttgtt
    36661 cctagtcaga ttaactacgt gatcaagaca cgctttacgg aaagtagagg ctacttggtt
    36721 gaaagtaagc gtggtggcgg aggctacatt cgtataggac ggattgagtt ttctagtcat
    36781 catgaaatgc tccgggagct gctttactcg attggtgagc gagtcagtca agaaatttat
    36841 gaggatattc tccagctttt ggttgagcag gaattgatga ccaagcagga gatgaatttg
    36901 ctagtatcag tagctttgga tcgcgtttta ggagaagaag ctccagttgt tcgagcaaac
    36961 atgctacgtc aggtcataca agaggtagat agaaaaggga agtaagatga actattcaaa
    37021 agcattgaat gaatgtatcg aaagtgccta catggttgct ggacattttg gagctcgtta
    37081 tctagagtcg tggcacttgt tgattgccat gtctaatcac agttatagtg tagcaggggc
    37141 aactttaaat gattatccgt atgagatgga ccgtttagaa gaggtggctt tggaactgac
    37201 tgaaacggac tatagccagg atgaaacctt tacggaattg ccgttctccc gtcgtttgca
    37261 ggttcttttt gacgaagcag agtatgtagc gtcagtggtc catgctaagg tgctagggac
    37321 agagcacgtc ttctatgcga ttttgcatga tggcaatgcc ttggcaactc gtatcttgga
    37381 gagggctggt ttttcttatg aagacaagaa agatcaggtc aagattgctg ctcttcgtcg
    37441 aaatttagaa gaacgggcag gctggactcg tgaagatctc aaggctttac gccaacgcca
    37501 tcgtacagta gctgacaagc aaaattctat ggccaatatg atgggcatgc cgcagactcc
    37561 tagtggtggt ctcgaggatt atacgcatga tttgacagag caagcgcgtt ctggcaagtt
    37621 agaaccagtc atcggtcggg acaaggaaat ctcacgtatg attcaaatct tgagccggaa
    37681 gactaagaac aaccctgtct tggttgggga tgctggtgtc gggaaaacag ctctggcgct
    37741 tggtcttgcc cagcgtattg ctagtggtga cgtgcctgcg gaaatggcta agatgcgcgt
    37801 gttagaactt gatttgatga atgtcgttgc agggacacgc ttccgtggtg actttgaaga
    37861 acgcatgaat aatatcatca aggatattga agaagatggc caagtcatcc tctttatcga
    37921 tgaactccac accatcatgg gttctggtag cggaattgat tcgactctgg atgcggccaa
    37981 tatcttgaaa ccagccttgg cgcgtggaac tttgagaacg gttggtgcca ccactcagga
    38041 agaatatcaa aaacatatcg aaaaagatgc ggctctttct cgtcgtttcg ctaaagtgac
    38101 gattgaagaa ccaagtgtgg cagatagtat gactatttta caaggtttga aggcgactta
    38161 tgagaaacat caccgtgtac aaatcacaga tgaagcggtt gaaacagcgg ttaagatggc
    38221 tcatcgttat ttaaccagtc gtcacttgcc agactctgct atcgacctct tggacgaagc
    38281 agcagcaaca gtgcaaaata aggcaaagta tgtaaaagca gacgattcag atttgagtcc
    38341 agctgacaag gccctgatgg atggcaagtg gaaacaggca gcccagctaa tcgcaaaaga
    38401 agaggaagta cctgtctaca aagacttgat gacagagtct gatattttga ccaccttgag
    38461 tcgcttgtca ggaatcccag ttcaaaaact gactcagact gacgctaaga aatatctaaa
    38521 tcttgaagca gaactccata aacgggttat cggtcaagat caagctgttt caagtattag
    38581 tcgtgcgatt cgccgtaatc agtcaggaat tcgcagtcac aagcgtccga tcggttcctt
    38641 tatgttccta ggacccacag gtgtcggtaa gaccgagttg gccaaggctt tggcagaagt
    38701 tctctttgat gacgaatcag cccttatccg ctttgatatg agtgagtata tggagaaatt
    38761 cgcagccagc cgtctaaatg gagctcctcc aggctatgtg ggttacgaag aaggtgggga
    38821 gttgacagag aaggttcgca ataaacccta ttccgttctc ctctttgatg aggtagagaa
    38881 ggctcaccca gatatcttta atgttctctt gcaggttctg gatgacggtg tcttgacaga
    38941 tagcaaggga cgcaaggtcg atttttcaaa taccattatc attatgacat cgaatctagg
    39001 tgcgactgcc cttcgtgatg ataagactgt tggttttggg gctaaggata ttcgttttga
    39061 ccaggaaaat atggaaaaac gcatgtttga agaactgaaa aaagcttata gaccggaatt
    39121 catcaaccgt attgatgaga aggtggtctt ccatagccta tctagtgacc atatgcagga
    39181 agtggtgaag attatggtca agcctttagt ggcaagtttg gctgaaaaag gcattgactt
    39241 gaaattacaa gcttcagctc tgaaattgtt agcaaatcaa ggatatgacc cagagatggg
    39301 agctcgccca cttcgcagaa ccctgcaaac agaagtggag gacaagttgg cagaacttct
    39361 tcttaaggga gatttagtgg caggcagcac acttaagatt ggtgtcaaag caggccagtt
    39421 aaaatttgat attgcataaa agaataaaag tatcagcatc tgaccataag tcacagtgga
    39481 gtgaaactca atgaaaatca aagagcaaac taggcagcta gccgcaggtt gcttaaaaca
    39541 ctgttttgag gttgcagata gagctgacgt ggtttgaaga gattttcgaa gagtatgaaa
    39601 ctaaaaccta tagcttctaa acgattcgtg gttttcacca ttcaacacaa aattcatatg
    39661 tttattaccc tccgtcgtat ttgtcttaga gcgtgtgtag tagaaaaaga gcagtcttat
    39721 ctgaaatttt tattctttca aaagagacct gtttcttttt tgcatgtcaa atccgttcta
    39781 actggtattt gaaaaatcaa actaatactc aatgaaaatc aaagaacaaa ctaggcagct
    39841 agccgcaggt tgctcaaaac actgttttga ggttgtagat agagctgacg tggtttggag
    39901 agattttcga agagtataag ctgcaagatg aatgattttc ttgtattgac gttgttgttg
    39961 acaaaaagta gcggataaat gaaatccatt ccattatcat agatgatagg ctggtaggaa
    40021 attttcaaat agcatacagg aaatagatgt atggagttct ggtagtagaa agggagagag
    40081 atgaacattt tagttgcaga tgacgaggaa atgattagag aaggaattgc agcatttctg
    40141 acagaagagg gttatcatgt cattatggct aaggatggac aagaggtctt ggaaaaattt
    40201 caagatctcc ctatccatct catggtactg gatttaatga tgcctaggaa gagtggtttt
    40261 gaagtgttaa aagaaatcaa tcaaaagcac gatattcctg tcatcgtctt gagtgctctg
    40321 ggagatgaaa ctactcagtc acaggtattt gatctctatg ctgatgatca tgtgacaaaa
    40381 cctttttctt tggtactgct tgtcaagcgt attaaggcgc ttatcagacg ttactacgtc
    40441 atagaggata tttggcgata tcaggatgta acagtggatt ttacctctta caaagcacat
    40501 tataaaaatg aagaaattga tctcaaacca aaggaattac tggtactaaa gtgtttgatt
    40561 cagcataaaa atcaagtttt aagtagagag cagatattgg aagaaatttc aaaagatgta
    40621 gctgatttac cttgtgatag ggtcgttgat gtctatattc gtactcttcg caaaaaatta
    40681 gctttagatt gtatcgtgac tgtgaaaaat gttgggtata agattagctt atgataaaaa
    40741 atcctaaatt attaaccaag tcttttttaa gaagttttgc aattctaggt ggtgttggtc
    40801 tagtcattca tatagctatt tatttgacct ttccttttta ttatattcaa ctggaggggg
    40861 aaaagtttaa tgagagcgca agagtgttta cggagtattt aaagactaag acatctgatg
    40921 aaataccaag cttactccag tcttattcaa agtccttgac catatctgct caccttaaaa
    40981 gagatattgt agataagcgg ctccctcttg tgcatgactt ggatattaaa gatggaaagc
    41041 tatcaaatta tatcgtgatg ttagatatgt ctgttagtac agcagatggt aaacaggtaa
    41101 cagtgcaatt tgttcacggg gtggatgtct acaaagaagc aaagaatatt ttgcttttgt
    41161 atctcccata tacatttttg gttacaattg ctttttcctt tgttttttct tatttttata
    41221 ctaaacgctt gctcaatcct cttttttaca tttcagaagt gactagtaaa atgcaagatt
    41281 tggatgacaa tattcgtttt gatgaaagta ggaaagatga agttggtgaa gttggaaaac
    41341 agattaatgg tatgtatgag cacttgttga aggttattca tgagttggaa agtcgtaatg
    41401 agcaaattgt aaaattgcaa aatcaaaagg tttcctttgt ccgtggagca tcacatgagt
    41461 tgaaaacccc tttagccagt cttagaatta tcctagagaa tatgcagcat aatattggag
    41521 attacaaaga tcatccaaaa tatattgcaa agagtataaa taagattgac cagatgagcc
    41581 acttattgga agaagtactg gagtcttcta aattccaaga gtggacagag tgtcgtgaga
    41641 ccttgactgt taagccagtt ttagtagata ttttatcacg ttatcaagaa ttagctcatt
    41701 caataggtgt tacaattgaa aatcaattga cagatgctac cagggtcgtc atgagtctta
    41761 gggcattgga taaggttttg acaaacctga ttagtaatgc aattaaatat tcagatgaaa
    41821 atgggcgcgt aatcatatcc gagcaagatg gctatctctc tatcaagaat acatgtgcgc
    41881 ctctaagtga ccaagaacta gaacatttat ttgatatatt ctatcattct caaatcgtga
    41941 cagataagga tgaaagttcc ggtttgggtc tttacattgt gagtaatatt ttagaaagct
    42001 atcaaatgga ctatagtttt ctcccttatg aacacggtat ggaatttaag attagcttgt
    42061 agacagatta gttttttatt aaagttcaca tagggttaac ataagtgtat tattctttat
    42121 atagataaaa atatagataa aagaaaggat actaatatgg tattagcgat tattttagta
    42181 acattcctta ttcgattgat ttttttaaag cgttcgatag agaatgagaa acgaatcctt
    42241 agcaatggcg ggaaagaatt tggagttgag aatacaaaac gattaacgtt ggctcatatt
    42301 gttttttatc tctcttgcct cgttgaagca atggtgcata agacaatgtt tgatggtatg
    42361 agcatggttg gtttagtctt gcttattttt tctatgctga tgttgatgtt ggtgattcac
    42421 ttgttgggag atatttggac agtgaagctt atgcttgtca ataatcacaa atatgtagat
    42481 catatcttgt ttaggacagt aaaacatcct aattactttt taaatatttt acctgagttg
    42541 attggcttga ccttgttgag tcatgcctat atgacttttg ttttagtttt tccagtttat
    42601 gcagttattt tgtatcgacg aatagctgaa gaggaaaagc tattacatga agttataatc
    42661 ccaaatggaa gcataaagag ataaatacaa aattcgattt atatacagtt catattgaag
    42721 tgatatagta aggttaaaga aaaaatatag aaggaaataa acatgtttgc atcaaaaagc
    42781 gaaagaaaag tacattattc aattcgtaaa tttagtattg gagtagctag tgtagtagtt
    42841 gctagtcttg ttatgggaag tgtggttcat gcgacggaga atgagggaat tacccaagta
    42901 gccacttctt ataataaggc aaatgaaagt cagacagaac ataggaaagc tgctaaacaa
    42961 gtcgatgaag atataaaaaa aatgttgagt gagatccaag aatatataaa aaaaatgttg
    43021 agtgagatcc aattagataa aagaaaacat acccaaaatg tcaacttaaa cagaaagttg
    43081 agcgcaattc aaacgaagta tttgtatgaa ttaagagttt taaaagagaa gtcgaaaaaa
    43141 gaagagttga cgtcaaaaac aaaaaaagag ttagacgcag cttttgagaa gtttaaaaaa
    43201 gaaccagaac ttactaaaaa attagcagaa gctaaacaaa aagccaaggc tcaaaaagaa
    43261 gaagatttcc gtaactaccc aaccaatact tacaaaacgc ttgaacttga aattgctgag
    43321 ttcgatgtga aagttaaaga agcggagctt gaactagtaa aagaggaagc taaaccccga
    43381 aacgaggaaa aaattaagca agcaaaagcg aaagttgaga gtaaaaaagc tgaggctaca
    43441 aggttagaag aaatcaagac agaacgtaaa aaagcagaag aagaagctaa acgaaaagca
    43501 gaagaatctg agaaaaaagc tgctgaagcc aaacaaaaag tggatactaa agagcaaggt
    43561 aaaccaaaga ggcgggcaaa acgaggagtt tctggagagc tagcaacacc tgataaaaaa
    43621 gaaaatgatg cgaagtcttc agattctagc gtaggtgaag aaactcttcc aagcccatcc
    43681 cttaatatgg caaatgaaag tcagacagaa cataggaaag atgtcgatga atatataaaa
    43741 aaaatgttga gtgagatcca attagataga agaaaacata cccaaaatgt caacttaaac
    43801 ataaagttga gcgcaattaa aacgaagtat ttgtatgaat taagtgtttt aaaagagaac
    43861 tcgaaaaaag aagagttgac gtcaaaaacc aaagcagagt taaccgcagc ttttgagcag
    43921 tttaaaaaag atacattgaa accagaaaaa aaggtagcag aagctgagaa gaaggttgaa
    43981 gaagctaaga aaaaagccaa ggatcaaaaa gaagaagatc gccgtaacta cccaaccaat
    44041 acttacaaaa cgcttgaact tgaaattgct gagtccgatg tgaaagttaa agaagcggag
    44101 cttgaactag taaaagagga agctaacgaa tctcgaaacg aggaaaaaat taagcaagca
    44161 aaagagaaag ttgagagtaa aaaagctgag gctacaaggt tagaaaaaat caagacagat
    44221 cgtaaaaaag cagaagaaga agctaaacga aaagcagaag aatctgagaa aaaagctgct
    44281 gaagccaaac aaaaagtgga tgctgaagaa tatgctcttg aagctaaaat cgctgagttg
    44341 gaatatgaag ttcagagact agaaaaagag ctcaaagaga ttgatgagtc tgactcagaa
    44401 gattatctta aagaaggcct ccgtgctcct cttcaatcta aattggatac caaaaaagct
    44461 aaactatcaa aacttgaaga gttgagtgat aagattgatg agttagacgc tgaaattgca
    44521 aaacttgaag ttcaacttaa agatgctgaa ggaaacaata atgtagaagc ctactttaaa
    44581 gaaggtttag agaaaactac tgctgagaaa aaagctgaat tagaaaaagc tgaagctgac
    44641 cttaagaaag cagttgatga gccagaaact ccagctccgg ctcctcaacc agctccagct
    44701 ccagaaaaac cagctgaaaa accagctcca gctccagctc cagaaaaacc agctccggct
    44761 cctcaaccag ctccagctcc agaaaaacca gctgaaaaac cagctccagc tccagctcca
    44821 gaaaaaccag ctccaactcc agaaactcca aaaacaggct ggaaacaaga aaacggtatg
    44881 tggtacttct acaatactga tggttcaatg gcaacaggct ggctccaaaa caatggctca
    44941 tggtactacc tcaacagcaa tggcgctatg gcgacaggat ggctccaaaa caatggctca
    45001 tggtactacc tcaacagcaa tggcgctatg gcgacaggat ggctccaata caatggttca
    45061 tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggttccaata caatggttca
    45121 tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggttccaata caatggttca
    45181 tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggttccaata caatggttca
    45241 tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggctccaata caatggttca
    45301 tggtactacc taaacagcaa tggtgctatg gtaacaggat ggctccaaaa caatggctca
    45361 tggtactacc taaacgctaa cggttcaatg gcaacagatt gggtgaaaga tggagatacc
    45421 tggtactatc ttgaagcatc aggtgctatg aaagcaagcc aatggttcaa agtatcagat
    45481 aaatggtact atgtcaatgg ctcaggtgcc cttgcagtca acacaactgt agatagctat
    45541 agagtcaatg ccaatggtga atgggtaaac taaacttaat ataactagtt aatactgact
    45601 tcctgtaaga actctttaaa gtattcccta caaataccat atcctttcag tagataatat
    45661 acccttgtag gaagtttaga ttaaaaaata actctgtaat ctctagccgg atttatagcg
    45721 ctagagacta cggagttttt ttgatgagga aagaatggcg gcattcaaga gactctttaa
    45781 gagagttacg ggttttaaac tattaagcct tctccaattg caagagggct tcaatctctg
    45841 ctagggtgct agcttgcgaa atggctccac ggagtttggc agcgccagat gttccacgga
    45901 ggtagtgagg agcgaggccg cggaattcac gaactgcgac gttttctcct ttgaggttaa
    45961 tcaatcgttt caagtgttcg taggcgatct tcatcttgtc ttcaaaggtc aaatcaggta
    46021 ggatttctcc tgtttcaaag taatggttga tttggttgaa gaggtaagga tttcccatgg
    46081 cagctcggcc aatcatgact gcgtcagcac caacttcttc gatgcgttgc ttggcttctt
    46141 ggacagtacg gatatcaccg ttggcgatga atggaatctt ggttagagct tgggcaacct
    46201 tgtaaagggt ctcaaggtct gcgtggccag tatacatttg ttcacgggta cggccatgca
    46261 tggcgagggc agaaacacct gcagcttcag cagcgagggc attttctact gccagagatg
    46321 ggtccgccca gccggtacgc attttgacag taagtgggat atcaaggaca gactggacct
    46381 tgttgatgat agagtagatc ttgtcaggat ccttgagcca catagcacca gcttcgttct
    46441 tcacgatttt gttgacaggg cagcccatgt tgatatcgac gatatcggtc ttggtgtttt
    46501 cttggatgaa ttctgctgcg cgtgctaggc tgtcttcatc gctaccaaaa agttggatag
    46561 agacagggtt ttcgccctca tcgatatgaa gcatgtgcag ggttttttcg ttgttgtatt
    46621 ggattccctt gtcagagacc atttccatta caacgagtcc agctccgagc tcttttgcga
    46681 tggtacgaaa ggctgagttg gtcacgccag ccataggcgc taaaacggta cgattgggaa
    46741 tctcaacatt gccaatcata aaaggtgtat taagatttgt cacgaatgag ttcctccagg
    46801 tccttttcat caaagttgta agtagtttgg cagaattgac aagtgatttc tgccccgtgg
    46861 tcttcctctt tcatttcctg taagtctgag cttggaaggc tggcaagagc gttcataaag
    46921 cgttcatggc tacagtcaca ttggaaacgg atttcttctt cagaaagacg cttgtaggct
    46981 tcgtccccgt agatagcctt gaggagggct tcgatatggt cgtcgctttc gagaagagta
    47041 gagatagctg gcatttcttg gatgcgtttt tcaaagcgag caatctcttc tttcttggct
    47101 cctggcaaga cttgaactag gaaaccacct gcaaccttga ccttgtcttc ctcgtccaaa
    47161 aggacattga ggccgaccgc tgaaggcgtt tgttggcttt cagtaaggta aaaggcaagg
    47221 tcttcaccga tttctccaga gatgagggga gttatagagt tgtaaggatt tccagtaccg
    47281 tagtctgtga taacgaggaa ttgaccattt ccaacaaaag gtccgactag gacttcacca
    47341 gtcgcagtct ttttgatgtc aacaccagga ttttgaacat agcctttgac gttccccttg
    47401 gtatcagcga cggtgataat agcacctaga gagctagagc ccaacacctt aactgtaagt
    47461 ttggtatttc ctttttcatt ggctgcgaga atctggctag cgataagagt tcgaccaagc
    47521 gctacagttg agctagcttg ggtttgatgt ttttcttgag cagtgcggac ggtttcagtg
    47581 ctatcaagga caaaagcacg aaaggctccg ctttctgata tagttttaat aattttatcc
    47641 atagctacta ttttagcata aaaatgccca aagggggagc cgtgtgttta ctgattttca
    47701 ggataatgga ccaggaaatc agcatgaaaa taaaaagaga aacagattat tttagcattt
    47761 gtaagattta tgctatgctt aaggtagaaa atgaaagggg taacaaatgt atttaggaga
    47821 tttgatggag aaagccgagt gtggtcaatt ttcaatcctt tcctttctat tacaagagtc
    47881 tcagacgacc gtcaaggctg taatggaaga aacaggattt tcaaaagcaa ccctaaccaa
    47941 atatgtcacc ctgctcaatg acaaggcttt ggatagtggc ttagaactga ctattcactc
    48001 agaagatgaa aatctgcgtc tgtctatcgg tgcagctacc aaggggagag atattcggag
    48061 cttgtttttg tatagtgctg ttaaatacca gattttggtt tatcttctct accaccaaca
    48121 gtttttagcc catcagctgg ctcaagaatt ggtgattagc gaggctacgc ttggtcgtca
    48181 cttggctggt ttaaatcaga ttttgtcaga atttgattta tccatccaaa atggacgctg
    48241 gagaggtcca gagcatcaga ttcgctattt ctatttctgt cttttccgaa aggtctggtc
    48301 gagtcaggaa tgggaaggtc acatgcagaa accagagaga aaacaggaga ttgccaattt
    48361 agaggaaatc tgcggtgcaa gtttgtctgc ggggcagaaa ttggacttgg ttctctgggc
    48421 tcacatcagt caacaacgtc ttcgggtcaa tgcttgtcag tttcaagtca tagaagagaa
    48481 aatgcgaggg tattttgaca atatctttta tcttcgtttg ctgagaaagg ttccgtcctt
    48541 ttttgctggg caacatattc cactaggagt tgaggatggt gagatgatga tattcttctc
    48601 ttttctccta tctcatcgca ttcttcctct tcatactatg gagtatattc ttggttttgg
    48661 agggcagttg gcagatttac tgacgcaatt gattcaagaa atgaagaagg aggaactatt
    48721 gggggattat acagaggacc atgtcaccta tgaactcagt cagctttgtg ctcaagtcta
    48781 tctctataag ggctatattt tacaggatcg ctacaagtac cagttagaga atcgtcatcc
    48841 atatttactg atggaacatg attttaaaga gacagcagag gagatttttc atgctctacc
    48901 tacttttcaa caggggacag atttagataa gaagattctc tgggaatggc tccagttaat
    48961 cgaatatatg gctgaaaacg gtggccagca tatgcggatt ggtctggatt tgacatctgg
    49021 ttttcttgtc ttttcaagga tggcagccat tttgaaacgg tatttggaat acaatcgttt
    49081 tattaccatt gaagcctatg accctagtag gcattatgat ttgctggtta ccaataaccc
    49141 tattcataag aaggaacaga caccagtcta ttatttaaaa aatgacttgg atatggaaga
    49201 tttggcaggg attcgtcagt tattattcac ttaaaaggct tggttaatcc aggtcttttt
    49261 tgtgaaattc acacaatctc ctcacatttt tttaaaaatt aaaaaaagtt gataaacaag
    49321 aaagcgcttt attttgtata ctagtaagtg taaagaggaa acacctcaag atctttatca
    49381 ggaggacagt acatgtcaca agaaaaatac atcatggcta ttgaccaggg aactacaagt
    49441 tctcgtgcca tcattttcaa caaaaaaggg gaaaaggtta gctcgagtca aaaagagttt
    49501 acccagattt tccctcaggc aggttgggtt gagcacaatg ccaatgaaat ttggaactct
    49561 gttcagtcag ttattgcggg tgctttcatt gaaagtggtg tcaagccaaa tcaaatcgag
    49621 gcaatcggga ttaccaacca acgtgaaaca acggttgtct gggataagaa aacaggactt
    49681 cctatctaca atgctatcgt ttggcagtca cgccagacag cacctttggc tgagcaacta
    49741 aaaagccaag gttatgtgga aaaattccat gaaaagactg gtttgattat tgatgcttac
    49801 ttctctgcta ccaaggttcg ttggattttg gatcatgtag aaggtgctca agagcgagca
    49861 gaaaaagggg aattgctctt tggtactatc gatacttggt tggtttggaa attgactgac
    49921 ggtgcggctc acgtgactga ctactcaaat gcagctcgta ccatgcttta taacattaaa
    49981 gaactcaaat gggatgatga gattttggaa atccttaaca ttccgaaggc tatacttcca
    50041 gaagttcgtt ctaactccga aatctacggc aagacagctc cattccattt ctacggtgga
    50101 gaggtgccaa tctcaggtat ggctggggac caacaagcag ccctctttgg acagttggct
    50161 tttgagccag gtatggttaa gaatacttat ggaacaggct ctttcatcat catgaatact
    50221 ggggaagaga tgcagttgtc tgaaaacaac ctcttgacaa ccattggtta cggaatcaac
    50281 ggtaaggttt attatgcctt ggaaggttct atcttcatcg caggaagtgc tattcagtgg
    50341 cttcgtgacg gtcttcgcat ggttgaaaat tcaccagaat ctgaaaaata cgctcgtgat
    50401 tctcacaaca acgatgaagt ttatgtcgtt ccagccttta caggtctagg cgctccatac
    50461 tggaaccaaa atgctcgtgg ttccgtcttt ggtttgactc gtggaacaag caaagaagac
    50521 tttatcaagg cgactttgca atctattgct tatcaagtgc gtgacatcat cgacactatg
    50581 caagtggata ctcagactgc cattcaagta ctgaaggtgg atggtggtgc agccatgaac
    50641 aacttcctca tgcagttcca ggcggatatt ttaggcattg acattgcacg tgctaaaaac
    50701 ttggaaacaa cagctctagg agcggccttc ctagcaggtt tatcagtagg atactggaaa
    50761 gacttggacg agttgaaact cttgaacgag acaggagaac tctttgagcc atctatgaac
    50821 gaatctcgca aggaacaact ctacaagggc tggaagaagg ctgtgaaagc aactcaagtc
    50881 tttgcggaag tagacgacta atactggcag aataaagcga tttatttaga aagtgtgtaa
    50941 atatggaatt ttcaaaaaaa acacgtgaat tgtcaattaa aaaaatgcag gaacgcaccc
    51001 tggacctctt gattatcggt ggaggaatca caggagctgg tgtagccttg caggcggcag
    51061 ctagcggtct tgagactggt ttgattgaaa tgcaagactt tgcagaagga acatctagtc
    51121 gttcaacaaa attggttcac ggaggacttc gttacctcaa acaatttgac gtagaggtgg
    51181 tctcagatac ggtttctgaa cgtgcagtgg ttcaacaaat cgctccacac attccaaaac
    51241 cagatccaat gctcttacca gtttacgatg aagatggagc aacctttagc ctcttccgtc
    51301 ttaaagtagc catggacttg tacgaccttt tagcaggtgt gagcaacaca ccaactgcga
    51361 acaaggtttt gagcaaggat caagtcttgg aacgccagcc aaacttgaag aaggaaggct
    51421 tggtaggagg tggagtgtat cttgacttcc gtaacaacga tgcgcgtctc gtgattgaaa
    51481 acatcaaacg tgccaaccaa gacggtgccc tcattgccaa ccacgtgaag gcagaaggct
    51541 tcctctttga cgaaagtggc aagattacag gtgttgtagc tcgtgatctc ttgacagacc
    51601 aagtatttga aatcaaggcc cgtctggtta ttaatacaac aggtccttgg agtgataaag
    51661 tacgtaattt gtctaataag agaacgcaat tctcacaaat gcgcccaact aagggagttc
    51721 acttggtagt ggattcaagc aaaatcaagg tttcacagcc agtttacttt gacacaggtt
    51781 tgggtgacgg tcgtatggtc tttgttctcc cacgtgaaaa caagacttac tttggtacaa
    51841 ctgatacaga ctacacaggt gatttggagc atccaaaagt aactcaagaa gatgtagatt
    51901 atctacttgg cattgtcaac aaccgcttcc cagaatccaa catcaccatt gatgatatcg
    51961 aaagcagctg ggcaggtctt cgtccattga ttgcagggaa cagtgcctct gactataatg
    52021 gtggaaataa cggtaccatc agtgatgaaa gctttgacaa cttgattgcg actgttgaat
    52081 cttatctctc caaagaaaaa acacgtgaag atgttgagtc tgctgtcagc aagcttgaaa
    52141 gtagcacatc tgagaaatat ttggatccat ctgcagtttc tcgtggatct agcttggacc
    52201 gtgatgacaa tggtcttttg actcttgctg gtggtaaaat cacagactac cgtaagatgg
    52261 ctgaaggagc tatggagcgc gtggttgaca tcctcaaagc agaatttgac cgtagcttta
    52321 aattgatcaa ttctaaaact taccctgttt caggtggaga attgaaccca gcaaatgtgg
    52381 attcagaaat cgaagccttt gcgcaacttg gagtttcacg tggattggat agcaaggaag
    52441 ctcattacct agcaaatctt tatggttcaa atgcaccgaa ggtctttgca cttgctcaca
    52501 gcttggaaca agcgccaggt cttagcttag cagacacctt gtctcttcac tatgcaatgc
    52561 gcaatgagtt gactcttagc ccagttgact tccttcttcg tcgtaccaat cacatgctct
    52621 ttatgcgtga tagcttggat agtatcgttg agccagtttt ggatgaaatg ggacgattct
    52681 atgactggac agaagaagaa aaagcaactt accgtgctga tgtaaaagca gctcttgctc
    52741 aaaacgactt agcagaatta aaaaattaag aaaaaataaa agaggtggag ggtagcaatc
    52801 cttgtcgccc gccccttttt taatggagac agaaaaatga tgagtgaatt atttggagaa
    52861 tttttaggga ctttaatcct gattcttcta ggaaatggtg ttgttgcagg tgtggttctt
    52921 ccaaaaacca agagcaacag tgcaggttgg attgtgatta ctatgggttg ggggattgca
    52981 gttgcggttg cagtatttgt atctggcaag ctcagtccag ctcatttaaa cccagctgtg
    53041 accatcggtg tggccttaaa aggtggtttg ccttgggctt ccgttttgcc ttatatctta
    53101 gcccagttcg caggggccat gctgggtcag attttggttt ggttgcaatt caaaccgcat
    53161 tatgaggtag aagaaaatgc aggcaatatc ctggcaacct tcagtactgg accagccatc
    53221 aaggatactg tatcaaactt gattagcgaa atccttggaa cctttgtttt ggtgttgaca
    53281 atctttgctt tgggtcttta cgattttcag gcaggtatcg gaacctttgc agtgggaact
    53341 ttgattgtcg gtatcggtct atcactaggt gggacaacag gttatgcctt gaacccagct
    53401 cgtgaccttg gacctcgtat catgcacagc atcttgccaa ttccaaacaa gggagacgga
    53461 gactggtctt acgcttggat tcctgttgta ggccctgtta tcggagcagc cttggcagtg
    53521 cttgtattct cacttttcta atctagaaaa caattatgtt gatagagctt gggcaagagc
    53581 ccaatttcag caaaaaatga agtaaatctt ctcataataa aacgcatcat atcaagcacg
    53641 aaaattccac gaggtcaact acagtcagaa agctgaacaa caagccaaaa cgcccaaaaa
    53701 aggcggcaaa aagcaagcac ctgcaagcaa cgtgccgaaa tagtcaaatc ctgattatgt
    53761 caacgaatta gacccaaaaa tcgttgatat gctagtagaa tttcacaagt cacaaggcac
    53821 tttggaaact cccgaggtgc aagcagaaat cgcccaaaaa cgtgaagaaa tcgagcaaag
    53881 gagagctgag cttgagggta aaaaacaaga gcttttgaac cgcttgaaca aatagagttt
    53941 cgcaagtatt atgcttacaa attacttgag caattaacta aaatataaac cctaccttta
    54001 tatctaggca gggtttatat tttagaaatt cacgtaggtt gttacggttt ttacataccc
    54061 agtatagttt gagtttctat agtattcagt gataaacttc cattttcttt gagcaacatg
    54121 gatataagta cttgttatgt agtatggata tgggctttgt gaatccaagt aagactgata
    54181 agcttgtata ccaaaatatg ctccaccaat cattgcaccc catggacccc ccaataaagc
    54241 acctatccta ccaatcatat aactgattcc agcaccagtc atgaagttag cgaatgtgtt
    54301 agcttgttta ttcccatgta ttgtgttgac gtaattccaa acattaggat cgtatgatct
    54361 aaaagatata tttaggtcga tttcattctt ttgataagcc atataaaatg ccccattgat
    54421 atagacgccg tcagcacgtc gttcaatagt gtctacactt ccatctggat tgacaacctc
    54481 aagaacttca tcgcttaaaa tatttacttg cgtatctccg aaccgcactg atgagccatt
    54541 ctcaaactga gcctcaccag atacaacttt agagtttgcc gataagctat catcagcaaa
    54601 aacaacaagc gacggggaaa tgctagacat acagaaaaca gacataacta gcaaacacat
    54661 gcatttaaac atcttagaca taacggaaac tcttttgtat ttttgatttt tttcaacttt
    54721 tattatacaa taaaaccaaa taaaaagaaa gcggtaacaa tatgcttaat gcgaaaattt
    54781 tttatatatt tttatgtttg atcgttatcg aaactacagg cttgttgttg ttgaaaagag
    54841 gtctcgaaat gggttattta gacacagaag ctattatcct cgcagttttt tcatttgctt
    54901 tttacaacct atgttcattc gcttgggtct gctctacaat aaaaaacaat aaaaaataaa
    54961 tagacgtatt ttcaaaaaaa aaaaatgcat atttatatta gcaaaacgac gatttaaatc
    55021 gtcgtttttt tgtagtacga cgggcatgtc gtatatctga ggtgtaagtc ctcggtgggc
    55081 acctgctacc ggtgaaccca atagcgattc ccaagcctga ctatcgtgag gtagcaggga
    55141 gaggaaggat agcgaaatcg tggctctacg aacaggaacg tgatagtaag gcgtatatag
    55201 cggataagga ggcttcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgtgtaaa
    55261 tcacgagagt aattgaattc ggactaaggt ttgtgtgaaa aagataaatc tttctagagt
    55321 ctaaagactc tgcgtcagat ttcctatttt cactgtaacc ttttaacgtc ctcatatctt
    55381 gtataaacga ggaaagatgt acgacttatc ccgtgaggtt tcatgagcgc tgaaagcgta
    55441 gtaacaacga atcatgagaa gtcagccgag cccatagtag tgaggaaact tctgtagtgg
    55501 aagtggagcg aaggggtgaa tactcaaaca gtctggggag agactgtttg aggtctgtcg
    55561 ctagaaagag aaaacgacag atcgaagtaa tcctacttca cttgtgtctg taaaatgagt
    55621 ggtctgatag aactggactt tgacacgtaa tgactagatg aaggttcacc aatataagat
    55681 gtccctcagg caccaaaaca agaaaggaat acgcacatgt caaaactgct agataagata
    55741 ttatcacgcg aaaatatgct ggaagcctac aatcaagtaa aatccaataa aggctcagct
    55801 gggattgatg gaatgactat cgaagagatg gataattatt tcagacaaaa ctggcgcttg
    55861 actaaggaac tgataaaaca gagaaaatat aagcctcaac cagttcttaa agttgagata
    55921 cctaaaccag acggaggcat ccgtcaacta ggaattccaa cagttatgga tagaatgatt
    55981 caacaggcca ttgtctaagt catgagcccc atttgtgaac cccatttctc agatacgagt
    56041 tatggtttca gaccaaatag gtcatgtgaa aaagccatca tgaagctctt agaatactta
    56101 aatgacggct atgagtggat agtggatata gacctagaga aatttttcga tacagttcct
    56161 caagatagat tgatgtcctt agtacataac attatcgaag acggagatac ggaatccttg
    56221 attcgtaagt atcttcattc aggtgttatc attaatggtc aacgttataa aacactagtt
    56281 ggtacaccac agggaggaaa tttatctcct ctcttatcca atatcatgct taatgaattg
    56341 gacaaggaat tagaaaagag gggacttcga tttgtgcgct acgcagatga ttgtgtgatt
    56401 acggtcggaa gcgaggagcc tctaagcgtg tgatgtattc agtcagtcgt tttattgaga
    56461 aacggctagg tttgaaagta aacatgacca agactaagat taccagacca agagagttga
    56521 aatatctagg ttttgggttc tggaaatcat cagatggttg gaaaagccgt ccacatcaag
    56581 atagtgttcg gagatttaag tttaaattga agaaactaac acagaggaaa tggagtatag
    56641 acctaacaag acgtattgag caactgaatt tgtctattcg aggatggata aactatttct
    56701 cattgggaaa tatgaaaagt atagtcgcca gcatagatga gcgcttgcgt actcgcctac
    56761 gagtgattat ctggaagcaa tgga
//
LOCUS       DAYYQN010000005        55973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYYQN010000005 DAYYQN010000000
VERSION     DAYYQN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.005"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(85..447)
                     /locus_tag="ACHBRQ_000280"
     CDS             complement(85..447)
                     /locus_tag="ACHBRQ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFM9592019.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            461..787
                     /locus_tag="ACHBRQ_000281"
     CDS             461..787
                     /locus_tag="ACHBRQ_000281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592020.1"
                     /translation="MKTKEQTRKLASGCSKHCFEVVDGTDVVSSKHCFEVVDRTDVVS
                     SKHCFEVVDRTDVVSSKHCFEVVDRTDVVSSKHCFEVVDRTDEVSNHTHGKATLTWFE
                     EIFEEY"
     gene            complement(918..1055)
                     /locus_tag="ACHBRQ_000282"
                     /pseudo
     CDS             complement(918..1055)
                     /locus_tag="ACHBRQ_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1433..2122)
                     /gene="rplA"
                     /locus_tag="ACHBRQ_000283"
     CDS             complement(1433..2122)
                     /gene="rplA"
                     /locus_tag="ACHBRQ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9592021.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2330..2755)
                     /gene="rplK"
                     /locus_tag="ACHBRQ_000284"
     CDS             complement(2330..2755)
                     /gene="rplK"
                     /locus_tag="ACHBRQ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9592022.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2887..3024
                     /locus_tag="ACHBRQ_000285"
     CDS             2887..3024
                     /locus_tag="ACHBRQ_000285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592023.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(3064..3780)
                     /gene="ldcB"
                     /locus_tag="ACHBRQ_000286"
     CDS             complement(3064..3780)
                     /gene="ldcB"
                     /locus_tag="ACHBRQ_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HFM9592024.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3783..4253)
                     /locus_tag="ACHBRQ_000287"
     CDS             complement(3783..4253)
                     /locus_tag="ACHBRQ_000287"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9592025.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLEKETRLRFLEEMSLIQEAVAKAFTAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(4255..5292)
                     /locus_tag="ACHBRQ_000288"
     CDS             complement(4255..5292)
                     /locus_tag="ACHBRQ_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9592026.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDGQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KEQNIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYIKSALVD"
     gene            complement(5390..6715)
                     /gene="brnQ"
                     /locus_tag="ACHBRQ_000289"
     CDS             complement(5390..6715)
                     /gene="brnQ"
                     /locus_tag="ACHBRQ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9592027.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6886..7173)
                     /locus_tag="ACHBRQ_000290"
                     /pseudo
     CDS             complement(6886..7173)
                     /locus_tag="ACHBRQ_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(7333..7911)
                     /locus_tag="ACHBRQ_000291"
     CDS             complement(7333..7911)
                     /locus_tag="ACHBRQ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFM9592028.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7965..9365)
                     /gene="pepV"
                     /locus_tag="ACHBRQ_000292"
     CDS             complement(7965..9365)
                     /gene="pepV"
                     /locus_tag="ACHBRQ_000292"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9592029.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9378..9983)
                     /locus_tag="ACHBRQ_000293"
     CDS             complement(9378..9983)
                     /locus_tag="ACHBRQ_000293"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9592030.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10154..10954
                     /locus_tag="ACHBRQ_000294"
     CDS             10154..10954
                     /locus_tag="ACHBRQ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9592031.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11136..11969)
                     /locus_tag="ACHBRQ_000295"
     CDS             complement(11136..11969)
                     /locus_tag="ACHBRQ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HFM9592032.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDCVLEVLDGQYGLENPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11959..13803)
                     /gene="uvrC"
                     /locus_tag="ACHBRQ_000296"
     CDS             complement(11959..13803)
                     /gene="uvrC"
                     /locus_tag="ACHBRQ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9592033.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQGESLPQVAEERVDYQTEGNHNEP"
     gene            complement(13842..14543)
                     /locus_tag="ACHBRQ_000297"
     CDS             complement(13842..14543)
                     /locus_tag="ACHBRQ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HFM9592034.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCALAFGFLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWERTGQLPKLNQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYVEAIQNWNQTGAFNFEIVTESSTADILATEMNNGNTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPNYGYSYERLVHTAEHELGHAIGLDHTDGKSVMQPAGSFYGIQ
                     EEDVTNLRKIYETSE"
     gene            complement(14622..15854)
                     /gene="murN"
                     /locus_tag="ACHBRQ_000298"
     CDS             complement(14622..15854)
                     /gene="murN"
                     /locus_tag="ACHBRQ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HFM9592035.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15859..17079)
                     /gene="murM"
                     /locus_tag="ACHBRQ_000299"
     CDS             complement(15859..17079)
                     /gene="murM"
                     /locus_tag="ACHBRQ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HFM9592036.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17191..17970)
                     /locus_tag="ACHBRQ_000300"
     CDS             complement(17191..17970)
                     /locus_tag="ACHBRQ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9592037.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(18039..19700)
                     /locus_tag="ACHBRQ_000301"
     CDS             complement(18039..19700)
                     /locus_tag="ACHBRQ_000301"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9592038.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19875..22097)
                     /gene="recJ"
                     /locus_tag="ACHBRQ_000302"
     CDS             complement(19875..22097)
                     /gene="recJ"
                     /locus_tag="ACHBRQ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9592039.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYRFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22374..23132
                     /locus_tag="ACHBRQ_000303"
     CDS             22374..23132
                     /locus_tag="ACHBRQ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9592040.1"
                     /translation="MALVEFKNVEKYYGDYHALRDINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LSPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDMTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            23172..23936
                     /locus_tag="ACHBRQ_000304"
     CDS             23172..23936
                     /locus_tag="ACHBRQ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9592041.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23949..24626
                     /locus_tag="ACHBRQ_000305"
     CDS             23949..24626
                     /locus_tag="ACHBRQ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592042.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24636..25295
                     /locus_tag="ACHBRQ_000306"
     CDS             24636..25295
                     /locus_tag="ACHBRQ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592043.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25590..26780
                     /locus_tag="ACHBRQ_000307"
     CDS             25590..26780
                     /locus_tag="ACHBRQ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HFM9592044.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(27101..27982)
                     /locus_tag="ACHBRQ_000308"
     CDS             complement(27101..27982)
                     /locus_tag="ACHBRQ_000308"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9592045.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(28123..29451)
                     /gene="vncS"
                     /locus_tag="ACHBRQ_000309"
     CDS             complement(28123..29451)
                     /gene="vncS"
                     /locus_tag="ACHBRQ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HFM9592046.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(29448..30104)
                     /gene="vncR"
                     /locus_tag="ACHBRQ_000310"
     CDS             complement(29448..30104)
                     /gene="vncR"
                     /locus_tag="ACHBRQ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HFM9592047.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30153..30239)
                     /gene="pep27"
                     /locus_tag="ACHBRQ_000311"
     CDS             complement(30153..30239)
                     /gene="pep27"
                     /locus_tag="ACHBRQ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HFM9592048.1"
                     /translation="MYEKGFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(30415..31794)
                     /gene="vex3"
                     /locus_tag="ACHBRQ_000312"
     CDS             complement(30415..31794)
                     /gene="vex3"
                     /locus_tag="ACHBRQ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HFM9592049.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31846..32493)
                     /gene="vex2"
                     /locus_tag="ACHBRQ_000313"
     CDS             complement(31846..32493)
                     /gene="vex2"
                     /locus_tag="ACHBRQ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HFM9592050.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32506..33783)
                     /locus_tag="ACHBRQ_000314"
     CDS             complement(32506..33783)
                     /locus_tag="ACHBRQ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592051.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELEMVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(34139..34309)
                     /locus_tag="ACHBRQ_000315"
                     /pseudo
     CDS             complement(34139..34309)
                     /locus_tag="ACHBRQ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(34452..35051)
                     /locus_tag="ACHBRQ_000316"
     CDS             complement(34452..35051)
                     /locus_tag="ACHBRQ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592052.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNTK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(35188..36072)
                     /locus_tag="ACHBRQ_000317"
     CDS             complement(35188..36072)
                     /locus_tag="ACHBRQ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9592053.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(36076..36462)
                     /locus_tag="ACHBRQ_000318"
     CDS             complement(36076..36462)
                     /locus_tag="ACHBRQ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9592054.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(36455..37798)
                     /gene="cysS"
                     /locus_tag="ACHBRQ_000319"
     CDS             complement(36455..37798)
                     /gene="cysS"
                     /locus_tag="ACHBRQ_000319"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9592055.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(37885..38769)
                     /locus_tag="ACHBRQ_000320"
     CDS             complement(37885..38769)
                     /locus_tag="ACHBRQ_000320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592056.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYQLAKLSNLEVLLADSSY
                     QEQISQLKFQAFSGEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(38781..39398)
                     /gene="cysE"
                     /locus_tag="ACHBRQ_000321"
     CDS             complement(38781..39398)
                     /gene="cysE"
                     /locus_tag="ACHBRQ_000321"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFM9592057.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(39414..41627)
                     /gene="pnp"
                     /locus_tag="ACHBRQ_000322"
     CDS             complement(39414..41627)
                     /gene="pnp"
                     /locus_tag="ACHBRQ_000322"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9592058.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(42314..43180)
                     /gene="metF"
                     /locus_tag="ACHBRQ_000323"
     CDS             complement(42314..43180)
                     /gene="metF"
                     /locus_tag="ACHBRQ_000323"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HFM9592059.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(43244..45493)
                     /gene="metE"
                     /locus_tag="ACHBRQ_000324"
     CDS             complement(43244..45493)
                     /gene="metE"
                     /locus_tag="ACHBRQ_000324"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM9592060.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLADHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKAEDFVDSLVAAYQEVFAKLAELGASRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVKLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPAILNHFAFAVEKLDEIRDLDAIRNGQGEEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(45754..46020)
                     /locus_tag="ACHBRQ_000325"
     CDS             complement(45754..46020)
                     /locus_tag="ACHBRQ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9592061.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            complement(46146..46549)
                     /locus_tag="ACHBRQ_000326"
                     /pseudo
     CDS             complement(46146..46549)
                     /locus_tag="ACHBRQ_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            46705..47706
                     /locus_tag="ACHBRQ_000327"
     CDS             46705..47706
                     /locus_tag="ACHBRQ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9592062.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(47879..50284)
                     /gene="pheT"
                     /locus_tag="ACHBRQ_000328"
     CDS             complement(47879..50284)
                     /gene="pheT"
                     /locus_tag="ACHBRQ_000328"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9592063.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIEFSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVSLLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(50361..50870)
                     /locus_tag="ACHBRQ_000329"
     CDS             complement(50361..50870)
                     /locus_tag="ACHBRQ_000329"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592064.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(50870..51916)
                     /gene="pheS"
                     /locus_tag="ACHBRQ_000330"
     CDS             complement(50870..51916)
                     /gene="pheS"
                     /locus_tag="ACHBRQ_000330"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9592065.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHHHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(52508..53923)
                     /locus_tag="ACHBRQ_000331"
     CDS             complement(52508..53923)
                     /locus_tag="ACHBRQ_000331"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9592066.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(53936..55774)
                     /locus_tag="ACHBRQ_000332"
     CDS             complement(53936..55774)
                     /locus_tag="ACHBRQ_000332"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9592067.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     MKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(55792..>55973)
                     /locus_tag="ACHBRQ_000333"
     CDS             complement(55792..>55973)
                     /locus_tag="ACHBRQ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9592068.1"
                     /translation="YCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSEL
                     VYLMAHIRRLTKNLD"
BASE COUNT        16015 a        12624 c        10339 g        16995 t
ORIGIN      
        1 attcccaaaa ggttgctgta taatgcaaga catttttctt actgatttta cttttcctta
       61 ccctcaattt agtgagctaa taaattattc ttcttcaaaa ggttgagatt tatctagtaa
      121 ctctaaaaca ttaatggtag gtagcaacat tgcttcactg ctatcaattg ccgaaactat
      181 tgaaacaata gaacgtaaat aatgataaag aatagcagta ccatttacgt atagaatatc
      241 ggtgatattt tcatctatat ttatttcaaa atatcctatt acagttacag atattttttt
      301 catggcatct ttgtcaacta aagaaacact aagttttacg gctccatgac tataatcatc
      361 tgtatgacca accttaacta tgggatataa tatatcgaag gtaaatgtga acaaactgat
      421 taggaaattt aaaccaactt ctaacaatct gttatactca atgaaaacca aagagcaaac
      481 taggaaacta gcctcaggtt gctcaaaaca ctgttttgag gttgtagatg gaactgacgt
      541 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgtagtca gctcaaaaca
      601 ctgttttgag gttgtggata gaactgacgt agtcagctca aaacactgtt ttgaggttgt
      661 ggatagaact gacgtagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga
      721 agtcagtaac catacgcacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga
      781 gtattagaaa gctcgatgga ctattccaga ttcattccac tagatttcga aatcgagtag
      841 gggataatct ctagcccctc tcacaccacc gtacgtgccg ttcggcatac ggcggttcaa
      901 ctaactgtta acgcatgtca ttcaaggtaa taatccaaac acgaaaccag tccacgtttt
      961 tcaaggactg gttttgatat agcacgttta agtaccgact tctgagctac taattgatag
     1021 tggtcgcccc agccagatac cttatccgct atataggcct tattatcacg ttcctgttcg
     1081 tagagccacg atttcgctat cccttcctct gacgattctt atttccagcg tcagacttaa
     1141 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcagac ttgggaatcg
     1201 ctattgggtt caccggtagc aggtgcccac cgaggactta cacctcaaat atataataaa
     1261 atgaaataag aataggacga atcgatcagg acagtcaaat caatttctaa caatgtttta
     1321 gaagtagatg tgtactattc tagtttcaat ctactataca acatgcccgt cgtacatcaa
     1381 aaaaagttga gacatagcgt cttcaacttt tttacattac tttaactact gattaaagtg
     1441 agtttacgtc aactttgata ccaacacctt gagtagttgt gatagtcaag tttgttacgt
     1501 aagttccttt agctgtagct ggttttgctt tttggattgt ttcgttgaaa gctttaaagt
     1561 tttcaaccaa tttttcagct tcaaatgata ctttaccgat gattgcttga acgttacctg
     1621 cacggtcagc acggtaagtg attttaccac ctttagactc ttcaactgct ttagcaacat
     1681 ccattgttac agtaccagtt ttagggtttg gcatcaagtt acgtggtcca aggacacgtc
     1741 caagacgtcc aacaagagcc atcatatcag gtgtagcgat aactacgtcg aagtccaacc
     1801 aaccgtcgtt gattttagca acaaggtcat cttcaccaac aaagtctgca ccagcagctt
     1861 ttgcttcttc agcttttgca ccacgtgcga aaacaagaac acgtgaagtt ttaccagtac
     1921 cgtttggcaa taccattgct ccacggattt gttggtcagc ttttttaacg tcgatgttca
     1981 agttgtaagc aacttctaca gttgcatcaa attttgcaaa gttagtttct tttgcaagtg
     2041 ctacagcttc ttctacactg tatgcttttg tgctgtcgat tttctcaaga gcagcacgaa
     2101 gttgtttgct ttttttagcc attttctatt ctccttgtaa gtggttcaat cgattttcat
     2161 ctcccacgtc acttctcgaa atgatgaagt cttgcgggtt ttcagtctac tatttttctc
     2221 ttcctacggc cttaagctcc cttgctgtac ccaagtacaa gcctcagtca cttgcctagt
     2281 atgaaaagca actaatagac tgctgtgaga ttttctactg tgttattgat tagtcaacaa
     2341 cagtgaatcc catagaacga gcagtacctt cgatcatacg cattgcagac tctacgtttg
     2401 ctgcgttcaa atctggcatc ttagtttctg caatttcttg tacttgtgca cgagtaactg
     2461 tagcaacttt agttttatta ggtgtacctg atcctttttc aacacctgca gcttttttca
     2521 aaagaacagc agctggtggt gttttcgtaa cgaaagtaaa tgatttatct tcgtaaactg
     2581 agataacaac tggaatgatc ataccagctt ggtcagctgt acgagcgttg aactcttttg
     2641 tgaatcccat gatgttgata ccagcttgac caagagcagg tccaaccggt ggagctggtg
     2701 tagctttacc agcagggatt tgcaatttta caagtttttc gactttttta gccattttta
     2761 aatcctcctt tgtggttttg gcggtaatta gagattttta cctcccacaa gtatgttttt
     2821 cacataccca tccattatac actatttatc tggaattgca agagaaaagt ttatttttta
     2881 tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgctc aaaatacagc
     2941 tttgaggttg cagataaaac tgacgaagtc agctcaaaac actgttttga ggttgtgaat
     3001 agaaccgacg cagtcagtag gtaaggcgac gctgacgtgg tctgaagaga ttttcgaaga
     3061 gtattaatcg acgtagtctc cgccttcaaa gccatagtat tcttccaaac tgagaccgct
     3121 tgcagcaatt tctttagctt cttttcctac ataacgcagg tgccattcct cagccatata
     3181 gcctgtttcc ttttccttgc ctttgagata acggacaaca aagccataat cagctgcatg
     3241 gtccaagagc cattgggctg ctttttcttc tgtcaccaaa tcaccatcag tcccaatcac
     3301 atcaaaggcc aagcctgtct ggtgttcgct atagccagga cgggcagagt aacggtcagc
     3361 tgctgccttt ccatcttggt tgacataatc ttgatagagc ttggtctgag tttcataact
     3421 tctaaaacca ctgtaatgat cactaatagg gaaacctgcc tcttgcatcg ctttgatgag
     3481 tttgaccaac tcagccttgg ctgttggatt ttccccggga ttatagtctt tcgacaaagg
     3541 ataatgttta ttggctacga tgatttcgtc gtattttcct tggatgctgt agtaatcacc
     3601 tttattgacc acttctgctt ttttctgggc agccccttga gacttactgc cgactgtccc
     3661 atcagctttg gctgtctgtt ctgtcttagc tgtgccatct tcattttttg ctttttcttg
     3721 tgaacaagct gctagactca aggctagcaa ggctgtcacg acaaggtatc ttttcttcat
     3781 ttctactcct ttatttctaa tagtttatct acttctgatg ataaacgttt gactaatctt
     3841 ttgatctcat ccggttttgc ttggcaggtt tctgctgtca tttcttcaaa aggaacccac
     3901 caaactggac cacgtccctt gagaccgtga ccattcatat cacctgtccg tcgtggaaac
     3961 agatgccaat gaagatgggc atcgccattt cctaaaagtt caatattcat tttctcagca
     4021 gtaaaggcct tagcaactgc ctcttggatt agactcattt cttcgagaaa acgtagtctt
     4081 gtttcctttt ctaaatggtg caattccgtg acgtgttcct tggctagaaa gagactatag
     4141 cctgcaaaat actggtgatc tccaatcaca agatagcctg tttccaactc tttgacaaaa
     4201 tagggatttt ctcccttctt gatgagctca attctctggc atataagaca catattagtc
     4261 taccaacgcg ctcttaatgt aagcatcaac catacgctct gttgcgacca ccgagtcaat
     4321 atgagtacgc tcataagaat gactagactc gatacccgcg ccaaggaggg cgtgtttgac
     4381 ctctgcccct gcggacatgg ccgctgaagc atccgaacca taaaatggat agatgtccag
     4441 cttaaaagga atattttgct ctttcgccaa ggtcaccaag tgttgacgga agtcatagtg
     4501 ataagggcct gaggcatcct tgacacagat agacactgtg tactcatctg tttgctggcc
     4561 atcgcccata gctcccatat caacagccag atattcgact acctgagcag gaatgttaga
     4621 attagcacca tgtcctactt cttcaaaaac tgaaaaagca aaatgagttg ttactggcaa
     4681 ttcaatcttc tctttcttat aaatacgaag caggttgagc aaaatcgctg cgctgacctt
     4741 atcatccaaa tgacgagact tgataaaacc tgtatcagtc acaacagttc gtggatcgaa
     4801 gctgataaaa tcaccgacct caatcccaag agcacgagtt tctttttcat tgcttacttt
     4861 tacatccaaa cgcacttcca tattgtcctg cgtgcgttct gcagttcctg catccttgta
     4921 gacatggcaa gaagtttggt ggatgaggat ggttcctgat accttttgac ctgtgctagc
     4981 cacatgaacg gtacagtttt caccttcaat catgttccaa ggaaaaccac cgatacggtc
     5041 caatttaaga cgaccatctg gtttgacagc acgaacaata gcacccagcg tgtccacatg
     5101 ggcagtcaca tagcgatgtt gctcatcatt ttgacctttg atggtcacat tgacaccacc
     5161 cttggctgtg cgaactggct ggtaaccaaa accttctaga gtcttgacta aataattcga
     5221 aatttcacga gtgaagcccg ttggcgatgc gatggctgtc agttctttga tatattctac
     5281 tgtttgattc atttcttcac ctctttcgct acctattata acacatttat catcggataa
     5341 aaaaagaaaa tcactcctgt agacttggct acaaaagtga tctaagtgat tattccattt
     5401 caaaaacatc gctttcttgc ttgtttggta gaaccaatga gagcaagatt ccaacaatgg
     5461 ctggcaccaa ccatgggaga gatgccttgg caaaaggaag aacgctaaca agatttgcaa
     5521 gaaactcaac cttaaacgag cttcctagta cgcttgcaat ggcaataact gtaaccacag
     5581 caattgtcaa ctgcatacct ggttttgaaa gggcaacaaa tttgttgaca atgacaatca
     5641 taacgatagc aatcgtgatt gggtacaaga taaccagtac tggaattgag tacttgataa
     5701 tcgcatcaag acccaaattg gcaatagcaa atccaatcaa ggtaaaggct gtcgcataaa
     5761 ccttgtagct gatttgtggg aagcgctcat taaagaactc agctgttgac acaatcaaac
     5821 caacagtcgt tgtgaagcag gttacggtaa ccatagctgc aaggaagagt tgagctgttg
     5881 agccaaagat ttcttgagtg gcttgtgaca agatgtaaac acctggtgtt ccacccttca
     5941 tcgcttcagc tggtactggg aaatgatttc caagaaaacc taaaccgatg taaagagcgc
     6001 tgaaggcaag ggcaacaacg ataccaacaa cccaaatagt tgaaatgtat tctttcttac
     6061 ttgaaaatcc aagttgtttc aaggtttgaa ctgcgattac gctaaaggcc actgaggcaa
     6121 gggcgtccaa ggtattgtaa ccttctagga aacctgtacc aaaggcagaa gcttgataag
     6181 cagctgaagc agcttgagga cttgttccac catatttgat agctcccaga acgaccaaga
     6241 taacaatcaa aattgcaaag actggcgtta aaatacgtcc aatgcggtct aagatttttg
     6301 atggattaag cgaaatcaaa taggctgccg caaaatacag aaccgtaaat acaatcaagc
     6361 caagtccttt atttgcatcc gacaaaaggg ggctaatccc tacttcgtaa gctgttgtag
     6421 cagtacgtgg ggtagcaaag aatggaccga ttgacaagta aagaactgag aggtaaagag
     6481 tcgcaaacca aggcgctatc ttcgttgaaa tctcgtagat atatccttta ggatttagcg
     6541 ttccaataat aagggtcaag acggcgatac caacgcctga aaagacaaaa cctgcgatgg
     6601 caggaagaaa atgttctcca gatagagcac ctagagaagg cggaaaaatc aagttccccg
     6661 caccaaaaaa tattccaaac aggagcaaac ctgttagggt acctttttta gccataaaaa
     6721 tctccttcat atttataaaa tactgaccta gtataccacg attaggagtc tgaaaaaagc
     6781 atcacgaggc aaatattaaa tgttttcaat tttcttaaaa ataagaatta tctaaaaata
     6841 aaactctcct acccagtcca caccaagtag gagaaatgca aaattttaaa gttcttcaaa
     6901 ggctaacatg ccaccctgga cattgataac attataacct tgttctaata ggaattggca
     6961 agcacgcgcc gatctcattc cagatttgca aataatataa tgcaagcgat ctttgtccaa
     7021 ttaatcataa ctatcagcca attgactaag cggtaggttg tgggcacctt ctaaatgaag
     7081 agctgcaaac tcatccactt ctctcacatc cactaaagaa agctggtcgt tttggtaaag
     7141 ctggtaaaat gcgtcaaagg ctatttcttt cattcttttt cttctaatac tcttcgaaaa
     7201 tctcttcaaa ccacgtcagc gtcgccttac cgtgcgtatg ttaccgactt cgtcagtttt
     7261 atctgcaact tcaaagctgt actttgagca gcctacgact agcttcctag tttgctcttt
     7321 gattttcatt gactataaaa tggttttaat tctttttttc aaatctggca ctacttctgc
     7381 ctcaaaccaa ggatttttgg ccatccagat ttgatttcgt ggtgatgggt gaactagcgg
     7441 aaaataggct ggcagatagt ctttatagtg tttcaccctc tccgttacct tcccactgat
     7501 tttctcctgt aaatagtagg cttgggcata ttgcccaatc aagagggtta actgaatatc
     7561 aggcaattcc tgtaagagct gcggatgcca tttttctgca aaacctgtac gaggcggaag
     7621 atcacccgac ttgccatgtc ctggaaagta gaaatccata ggcaaaacag caaaataacc
     7681 tgaattgtaa aaggtatctt catccacacc tagccagtcc cgcaagcggt caccactttt
     7741 atctttccag taaaggcctg cttcttgagt tttaagtccc ggagcctgac cgatgatatt
     7801 gatgcgagca gtttttggcg ctgcaaagag aggctcaatg ccacgctctg tatagctggc
     7861 attctgcgaa tccgccatga tagcctgttt gattctttcg atttgagaca tctactttcc
     7921 ctccaaaaag aagtccaagc ataagatctc agacttctat cgttttattt gatcaattcg
     7981 taaatagctt cggcataaat tgctgctgct cggaagagat cgtccaaggc aataaattca
     8041 ttggcttggt gcatggtatc aattgagtct gggaacatag caccataggc aactccgcgc
     8101 tctaacaagc gaccaaaggt tccaccaccg atgacttgtt catgaccttg taggcctgtt
     8161 tgtttttcat agacattcaa caaggtttgc acaagtggat cttccattgg cacatagtga
     8221 ggggtatgac catgttctga tagactgaca gaagcaactg gcaagttttc taggattgat
     8281 ttgatttgtt ctggacttgt tccttttgga tagcggatgt taagggcaat ggtattatca
     8341 gcacttgttt catcgaagcg gaagactccg gcattcatag aaagagcacc catcttttca
     8401 tccacatgag caatcttgag attttcaccc tcatgatcgt tcaagagaat tttacctgcg
     8461 atgtcaaggt agtctttggc tggaccagca aagccaaact ggctgaggaa gagggcaagg
     8521 taagttgcgc cattgacacc tgaagcaggc atagcaccgt gggctgattt accaatgatc
     8581 gtcaccttgt atttgccagc ttcttcttgg agttctcctc taagtttgtg ttctgcaaca
     8641 aaggcatcta gtttagcttg caagtcagcc aaatcacctg aaacgactgc tgttgctgat
     8701 tctggtacca tattttcacg taaaccacct gtaaagctgt gaagacgggc agcacctgta
     8761 ttttctcctg caaagtggag gtattccgtg atatttcctt tttcaccatt gatgattgga
     8821 aattcagcat ctggtgagaa accgaaatct ggtttggcaa gtcctacgtg ctcaaagtag
     8881 tagtccatgt ctgcccagcc tgattcttcg tctgttccaa cgatgaagcg aactttctta
     8941 gaagttggaa gacccaattc tttgatgatt ttcaaaccat agtaacaagc tgttgtagga
     9001 cccttatcgt ccgaagcccc gcgcgcataa aggcgaccat ctttgatagt tggtgtgtaa
     9061 ggatctgtat cccaaccgct accagctgga accacgtcca tatgggcaaa gattccgaga
     9121 acttcttctc catcaccaaa ctcaaaatgt cctgcgtagt tatcgacatt cttagttgga
     9181 taaccatcac ggtctgcgat ttcaaggaat ttttccaagg cttttactgg accaggtcca
     9241 aatggatgtt gggcatcagc cttgctatca tcacgttctg agttgatttc caaaaggcta
     9301 aacaagtcag ccaagaggtc ttctttgcgt ttttctactt ctgctgtaaa atcaattgct
     9361 atcatttttt cttcttccta tcttttttcg atgatttcat ctactggcaa gcggtagctt
     9421 ggttccaatt tgtcgtctgt gtaacccact gtgatcaaga gttctgggcg gaaacggtct
     9481 tcaatttcca aaacctcatt gacttttgat ttgtcaaaac caaggataat attggaacca
     9541 attccttggt ctgtaagagc gagaaccaag ttcatagcaa ccaaacctgc attgagtgct
     9601 aggtagtcgc tgacttgttg ttcattgtag cgtgcaaatt cagctggcaa atttttcatg
     9661 aagtattgaa gttgttcttc tgagaaattg ttagcaccac caactcgggc aatcttacga
     9721 gcgcgtttgg caaggtctgt atctgtaaat agggcaatgg ttacaggtgc tgatgatacc
     9781 tgctcaaagt ttgaaccata agccaacttt gccagttcag catttttctc acgaacaact
     9841 acaaatttcc aaggctggct gttgtgggcg cttggcgcca aggttgcaat ttcgatagcc
     9901 gtacgcacat cttttggatc aacaggctta tcagtgaaat gcttggtcgc atgacgtttt
     9961 ttatttaatt caagaaattt cataatcgat ttccttttct aattctactg cttccattct
    10021 accataattt gaaagagctt gcagggattt ttcatgatta agtttttcta tcacagacat
    10081 aaaagatata tattggttta tcaggaaagt ttctgataaa ctagcaagta ctttacattt
    10141 gaatggagac agtatgaaaa aatttagcct attactagct atcctaccat ttttggttgc
    10201 ctgtgggaat caagctacac ccaaagagac tagcgctcaa aagacaatcg tccttgctac
    10261 agctggcgac gtgccaccat ttgactacga agataaaggc aatctgacag gcttcgatat
    10321 cgaagttcta aaggcagtag atgaaaaact cagcgactac gagattcaat ttcaaagaac
    10381 cgcctgggaa agtatcttcc caggacttga ttctggtcac tatcaggctg cggccaataa
    10441 tttgagttac acaaaagagc gtgctgaaaa atacctttac tcgcttccga tttccaacaa
    10501 tcccctcgtc cttgtcagca acaagaaaaa tcctttgact tctcttgacc agatcgctgg
    10561 taaaacaaca caagaggata ccggaacttc taacgctcaa ttcatcaata actggaatca
    10621 gaaacacact gataatcccg ctacaattaa tttttctggt gaggatattg gtaaacgaat
    10681 cctagacctt gctaacggag agtttgattt cctagttttt gacaaggtat ccgttcaaaa
    10741 gattatcaag gaccgtggtt tagacctctc agtcgttgat ttaccttctg cagatagccc
    10801 cagcaattat atcattttct caagcgacca aaaagagttt aaagagcaat ttgataaagc
    10861 gctcaaagaa ctctatcaag acggaaccct tgaaaaactc agcaatacct atctaggtgg
    10921 ttcttacctc ccagatcaat ctcagttaca ataacactct tcgaaaatct cttcaaacca
    10981 cgtcagcttt atctgcaacc tcaaagctgt gctttgagca acctacggct agcttcctag
    11041 tttgctcttt gattttcatt gagtataaga tatactaaaa gcgattggac cagccaatcg
    11101 cttttttagt ttgcattggc tattttagga aactcttaca aaaaatcaaa aaaattcttc
    11161 acatcctcta catacccatg cttttctatt aagctggcta aaagttccag attgtgcccc
    11221 tgatagttat cgtcacgacg cagttcttca tacatttcaa taaagggaag ccccttggac
    11281 ttggccaatt ctaactcagc ctcgtaatca taagcaactt tacgaaattg gatattgacc
    11341 aattccccat cttcaacttc tatcacggca tactgggcac ggtgattttt taacgcctcc
    11401 caattaaaat agggcatgcc aatcgaccct ggattgatga tttgttgccc ttgacttcca
    11461 taacgaagca actgcttgtg aacatgacca taaactgcca cgtccgtttc cgcatctagc
    11521 agttggtcaa atttctctgt atcattctca actagcaagt caccaccata gttcttattt
    11581 ggcaaattat gagagataga aaagcgcagt ccagcaattt ctttcttttc cagcaaaggc
    11641 aagcttcgta gccagacagt cgttgcagga tccattcgct ccatcaaaaa ctgagtcatt
    11701 cgcatgagct ggatttcttg cggattttcc aaaccgtatt gcccatccaa gacctccagg
    11761 acacaatcat cccaattgcc tcgaacactg gctgtgatag gaaggtcctt tagcagggcg
    11821 actaagtcat ttgcgcctgg accaggaaga aaaatatctc ccagaagcca atattcactg
    11881 gccccttgat ttttagcatc tgcaatcact gcttctagcg ccgtcgcatt gccatgaaca
    11941 tctgataaaa ttgcgatttt atggttcatt gtggtttcct tccgtttggt aatctactct
    12001 ttcttctgct acttgaggca aggattctcc ctgcgggttc aactttcttt gcacagcctc
    12061 tgctactgct cttggtactc caacttcgac aatctcatcc acactggctt ccttgatttt
    12121 ggtcagagac ttgaagtgcc tcatgagatt ttgcttgcgt ttaggtccca gaccatcaat
    12181 cccatccaat tgagatgaga aagaattttt ggagcgcagt tggcggtgga aggtaatggc
    12241 aaagcggtgc acctcatctt ggatgcgttg gaggaggaaa aattcctgag aattgcgaga
    12301 caagtccacc acctcaagcg gatctccaaa gagcagttca tgggtttggt gcttatcatt
    12361 cttttgcagc ccagcaattg gaatatccaa gcccagttcc tcttggataa cctgcttagc
    12421 gatattgact tgaccttgcc ccccatcaat cacaatcaaa tctggaggag tcaaagcctc
    12481 acgctgtact cgaccatagc gtctgcgaat gacctctctc atgctggcat agtcgtctgg
    12541 tccaacaacc gtttttatct tgtacttgcg gtaatccttc ttactcggtt taccgttgac
    12601 aaagaccacc atagccgaaa cagggctagt tcccatgata ttagagttat cgaaggactc
    12661 gatacgtact ggggtcggga tttggagcaa acgtcctaga ttttcaatag ctccttgggt
    12721 cttctcgaca gatttctcta gcaggttaaa tttctgctcc agactaacac gtgcattttt
    12781 tatggctaga ttgaccagct gttttttctc tccacgttgg ggtttgagaa tcttggtatc
    12841 caccaaagcc ttgacagctt cttcgtcaat atcctgcgga atcagtacct cattgggaac
    12901 taggtgagat ttttcttgat agaattgtcc tacataggtc aaaaaatcct catctggatc
    12961 attgaagtag gggaagagat tgacatcgcg ctcgatgagc ttaccctgac ggacaaagaa
    13021 aacctgcaca cacatccagc ccttatccac atagtagcca aagacatcgc gattttggag
    13081 atccttagcc atgacccgtt gcttggttcg aagcgttcca atagcctgaa tcaggtcacg
    13141 gtattccgcc gcacgttcaa actccatagt ctgggctgcc gctgccattt tgccctttag
    13201 ctcatcaatg attttgttat cctgcccttt cagaaaatca gaaacctcct gggccataga
    13261 cttgaaataa gtctcatcct tcttacagat ggtgtgggcc atgcattgac cgatatggta
    13321 ataaaaacag accttagagg gcgggttggt acacttacga aaagggaata tccgatccag
    13381 caaccgcttg atttcattgg ctgcccccac atcgggatag ggtccaaaat aaagacctcc
    13441 gtcctttttg acctgacgag tgataatcaa gcgaggatac cgctcattag tgattttgat
    13501 gaagggatag gacttatcgt ccttgagcat aatattgtat ttaggcttat tttccttgat
    13561 tagattgatt tctaggagaa gtgcctcaat attggactcc gtaacaataa attcaaaatc
    13621 cacaatctca gacaccagag cctctgtttt ggtatcatga cttccacgga aataggaccg
    13681 cacgcggtta cgcagatttt tagcctttcc tacatagata atggtgccgt ttttatcctt
    13741 gtgaatgtaa caaccagggc tggtcggcaa gagctctagt tttgatttaa tcaagttatt
    13801 cataattcca ttatagcaaa aaagtaggga aagattgttc cctactcact agtctcatat
    13861 atttttcgga ggtttgtaac atcctcttcc tgaataccat aaaaggaacc tgcaggttgc
    13921 atcacagact tcccatctgt atggtccaag ccaatcgcat gccccaactc atgttctgcc
    13981 gtatggacaa ggcgctcata ggaatagcca tagtttggat tggacagata atagtgattc
    14041 aaccgtaccg tgacagacaa gaattgcccc gtcaagagat tggtctgact ttccgcctct
    14101 cctgccacag gagtgtttcc gttgttcatc tcagtagcca agatatccgc cgtgctggac
    14161 tcagtcacaa tttcaaagtt aaaagcacca gtttgattcc aattctggat tgcttctaca
    14221 taagcctctt gaaagcgtga atccatctgg ggatccaagt aaatacgagc agaagcctgt
    14281 gcccaccttc cttcagagtg ttggtgacta tctgtctggt tcaatttagg cagctgccct
    14341 gttctttccc attggtcaat actttgttga ccaatcttta ctgaccgttc tgcttgcttt
    14401 agcgctcctt gaaaatcccc gttcagatac cagagaaaac caaaagcaag agcgcataag
    14461 agaacaacta tccaaaccag gcgccaaaac aaacgccaca caaaagaaaa gaaagcccct
    14521 atcaaacgaa aaagccagcg catgtctctc cacctttcta gaaaataaaa actattttac
    14581 taaaacaagc tgaaagaaaa ctaaatactg actttttcat cttaacgtcc tactattttt
    14641 ttgagtaact ggatagcttt gtattttaaa ggcgatggat ggtaacggaa agtacctgct
    14701 ttgcgtacaa tatagccatt aaaattctgt ttaaaacgca aaacaccatc acttccatca
    14761 aaaatccctt gaatgcctag gaagttgtat ttaggtattc cacgttttat gctttccaac
    14821 ataacatatt tttgaagcag tgcaggggca tagaacttat taaactcagt gtaggaacca
    14881 ctaaagagat aagtcgtttc ctgaggcata taaacaaata aactcccagc taaaacaata
    14941 tcttcttctc catatttttc aatcaagtct cgcgcttctg cttttcgaac ttcaaacgtt
    15001 tcaaattgac tagaatattc tctcagttga ttttgttttt tctcagaatg aggattttta
    15061 ctcaaatcaa gtcgcaactt gtccaagttt tcttctagtt tactttgttc accttgcaat
    15121 ttgctcatat agtccgaaaa atttaagctt gctatgagaa actccgcttg ttctccaaaa
    15181 gtatcataaa aatgctcata atattctaaa cttttatcac tatattctct acgttcagag
    15241 gtttcttttg ttatattctt aaaaatcgat agttcttcac gttttaactt tttcaaccga
    15301 atgccaaagg tttcagcctt tttcaccaag ggtttaccct ttttgctaaa acttttaagc
    15361 aaactctttt cagttaattc agttaaatct ttatagtata accaatctgg ttctccacct
    15421 gggtaacctg ttgttaagcc atcaaattga taacctaaat cagtcaaacc ttgaataata
    15481 ctttttttct cagcatctat tgaattacct tggctatcaa aagtttgata agtttcataa
    15541 ggttttacaa gcaactctaa tacaccattt tgcttggcat attcttttaa ctctgcataa
    15601 aaaactggaa gagcatcttg ttgggtataa atcggccccg aattgagttc catatgcaga
    15661 ccacccagca tgggcaagct ataaaccaga gctgcaactt gaatttctcc ttcttgtttc
    15721 aaagcaagat aaacaattcg agcccctctt ttttctagca aatcccccat ctggacagat
    15781 tgcataaagg aacgagaaga aacctgatca gaataagtct gaaactcttc tttcgtgagt
    15841 gttgttagtg ccatatactt actttctatg tttttttctt aatgttttac ggaaatcaag
    15901 agcaagtctt aacagaggat agagaggatg agtgggcatt gtaaattcac ccaagtattc
    15961 ttcaatcgtt ggattaaatt tttccttaaa atgataaagt ccaccattga gagagttttc
    16021 aacaccacct aaattttgcc aaaccatacc tcgctcaaag gcatagcgag ccgtttcata
    16081 ccatgttaaa attggtgcat tgtaacgttt aaaatcatca tccataccag catatatatt
    16141 gacagaggta gtaccaaatt ccaaactcaa agtagccgct aaaggaactc tcgcttgacc
    16201 tacatctata tattcctgca agaaggtcaa ttcctctaac aaacgttctt tttccttctt
    16261 ctgcgcttct acttttgaaa ttcgagtcga ctcagtaaac gtctcttcca aggctctatt
    16321 tttcgctaac tgttcttcta attcttgcga acgtttagaa acatccaagg tggccaaggt
    16381 gatataggcc ttgtccttaa aattatctaa caatttttta taataggctt cattcctcaa
    16441 atgaatctct tttcgcttct cagttttttt catcaactcc gaaaatgaat ctaatagttc
    16501 cagtccacca tattgaatct caagcccttt gtttcgtgct gttcgaatag cctgttttgt
    16561 tgacttggaa agtttatctt cttcaaaatt ttccttgtat attttcgcct gaatacgagg
    16621 ttgaatggtg tctcccattt cctccgtttt tcctgaccac cttactccca tttgttgcaa
    16681 actatcaata atagccagat tttcaggaaa ttctgtcttt tcctgattga ttaaactttg
    16741 agataggcaa atacttgggt caaaagtcac aaaaaccgct ctcttactgc gagcatagga
    16801 cttaatagac tgaatgacaa aattcaagag ttctttatcc ccataatcca atataggtcc
    16861 tcttgggatg taaaacattt tatagcctag cggaagagtt ctaatcaaaa tactagctgt
    16921 cgccagtaat ttttcttccc tgtaaacacc aaacttctca tgttgccaat tagacttaac
    16981 ttcctcccaa gcactacttt gcaatacatt ggctaattca tgttctttga caaactgatc
    17041 atattctaat gtgggaatgc caatttgata acggtacatt tcctactctc tttcctccag
    17101 tattttatta ctattctact actttcgttt gaaaagtcta gtgaaaacat attttatttt
    17161 agttctactc cccctatgct gaagttcaaa ctaagtcagt ctctcttcta atttgaaatc
    17221 aattggtagg gttgttaaaa aaacttccaa ttgtttttcc cagtagtacc actcgtgagt
    17281 tccagcacta tggctatagg tcacatcaaa acctagtttt ttgagatttt tcactgcgag
    17341 attattggct tcgtacaaga aatcctgttc gccacaccac gcccaaagtt tggtcttttt
    17401 atccgatttt ttagccagac tttcaagaga atagggacta gttgtccagt ctctaatctc
    17461 tccaaaaaca cctctccagt aggctggact tcccagattt tgactttcag gagaaaagtt
    17521 ttgaaagctg agggcacctg aaaaactagc tgcatgagaa aaacgatttg tcgtaagagc
    17581 cagtttgaag cagccgtagc ctcccataga aagaccagcg ataaaggtct tttcacgctt
    17641 gctagtcata ttagggaaga agcgtttcag aacctgtggc aattcctctg ctagagccgt
    17701 gtagtagtca aaaccatact gggtatcggt gtaccaacca ttgctggtat tgggcataac
    17761 aacgatgaga ttagttcctc gaagcaagcg ttctacattg gtccgcttaa gccaactatt
    17821 atgatttcca gacatcccgt gcaaaaggta caagacggga atatcttcac actctggttc
    17881 ttccactcga ttggcatcag ggtagaggac attcaccccc cactccatat ccaatacttg
    17941 tgagtaatac tcgattttca ttactgccat gattttctcc ttctattttt ctataagaaa
    18001 aagccgaaac tcgactttct ctctgtttat ttcaacgatt attttgcttc catgactact
    18061 ggtaaaatag ctggacgacg cttggtttgg tcaaagagat acttggtcaa attatctcga
    18121 accttccctt taagatctgc ccagtcaaag tcatctcctt gaagataatc ttctaccgtt
    18181 tggttaatca attctgaact ttcacggaga atatcgcgac tcttcttgag ataaacaaat
    18241 ccgcgcgtat gaacacgagc cctagccaca attttcttct cacgacggtt gactgtaata
    18301 gccacgatga aaattccatc ctctgacaag accttacggt cacgaagaac aacatttcca
    18361 acatcaccaa tggcattccc atcaatcaag atatctcctg ctgaaaccga tccagctgga
    18421 acaaagtctc cattctcgta agccatggtc gtcccctttt taggaatgaa gatgcgttct
    18481 ggcaacatcc caactgccat ggcagcctta gcgtgagcat ccaactcacg atactcccct
    18541 tggacaggga agaggtactt aggttgcaaa agattaatca tcagctgcaa atcacgcaca
    18601 tttccgtgcc ctgatacatg taaactttgg gtaatcaatt tgacaacccc acctgcctga
    18661 taaatcatat tttctacacg cgcaacaaag gcttctttag caatagacgg agccgtagca
    18721 atatagacca ggtccccatc cttgatttct acataacgat ggcgaccaat cgacatctta
    18781 cgaagtccat tgataggttc acccatacga cctgtctcaa gaataatcaa ctcatggtct
    18841 tcaaagcgag acatatcttt aggcttaatc aagagaattt cgttggctaa agacaacttc
    18901 ttaagacgaa tcgctgtgcg gacgatattt tcaatatcaa atcctgtcaa gacgatacgt
    18961 cgacctgttt tatccgcagc gtcaaaaatc tgctgaatac gagaaagatt actggaaaca
    19021 gctgcaacga tgatacgacc ttcccagtca gcaatagttt gggtaatttc atccctaact
    19081 tcactttcac tagccacctg aatattgctg tctgcattgg ccgaatcact gaggagagcc
    19141 aggacgccgt cacgaccaat ctctgccaaa cgagcaaaat cagttgcata agattcacta
    19201 gccgtttggt caaatttgaa gtcacctgta taaacgatgc ttccttccga tgtcttcaag
    19261 acaattccca gactctctgg aacggagtaa gtcgtaggga agaaggaaac cactgtccca
    19321 ccaaaatcaa tctccgtatt ctcatcaatg acatggaaat cattaaattt cttaacggca
    19381 tcatttcctt tgacaaagag ctttgccaac tcaatggtca actcagaccc aaatacagga
    19441 actttagcct ctgccaagag ataaggtaga gcaccaatgg catccgcatg cccgtgggtc
    19501 aagaaaaccc cagcaatacg gtcgctattt tcaaaaaggt aatccatgtt tggaatcacc
    19561 acatcgaccc ctaattgttc attttcagga tattttaacc ctacattcaa aacaaaaatg
    19621 gactctccaa tttcagcaat gtacatattt tttccattct cacgcacacc accaagtgtt
    19681 gttaaactga tattactcat ttttcctcca aaatcttagt ttatcttgat tatagggcaa
    19741 ctctaccctc ttattctaaa agttctgata ctttcagccg aatcttttcc taccattata
    19801 ccattttttt gatgattttc aaaatgaagg tctgttttca aaaaagagtt gatttcccaa
    19861 ctctttccta acttctactc tttttccatc aaaaaatcat aaatttcttg caccgtaccc
    19921 agcgccatca tttcttggtc tttaacggtt tgtttgagat tttggtaaat ttgactttct
    19981 cctatctccc gctttggcgc ctctttattg actgtcatca caccatcttt tatcgtcaca
    20041 aagcctagtt cttcaaatac ttgaatcatc ttgaccagca agatttgttg aatattaaga
    20101 tatgcagcca aatctttcag cttgtagcga atatcaaact ctgggaaccg gtaaatagtc
    20161 ttgtacaatt tggcaaactg atctctagtc ccataacctg tcagataata ggccttgtca
    20221 atatcatttt tgaaatagac agcagagaaa tgctgttcct gaaaaatggt cttcagctga
    20281 gtaatatcct ctggaatgtt ttttacgaca acagcttcac tagccgcaag atttggcagt
    20341 tctccaggaa aatccaagac tggaacacct tctggcaaga ctgcattttt tccacgaatg
    20401 ttaaaaagtt gaacaccttc cactcgcgca tccaccatca tcaactggag ggcagtttgg
    20461 ccattccatt ggttgacaga caatttaacc gctaactcta gattcttggt ttgagaaaac
    20521 tctgtcgccc atctgccttg accaaaggct accacttcaa aactcgcctc acccttggaa
    20581 attttcagct ttagatgggc attacctgcc cccatagtac gagcactttc gacctgaaaa
    20641 ttcttgatat aaaaaatagg tttctgatta tccattccaa aaggagctaa acgttcaaaa
    20701 cttttgaccg tttccaagct aagtgcctcc aaatccaact cttcatctag gtttaactta
    20761 ttcttgccac cagcatctgc acctttttca cgaacataat cttccaaaac ctgagataaa
    20821 tctgagagtt gctcaacttc cagcgtcata cccgctgcac ctgcatgacc tccaaaggcg
    20881 atgaagaggt ctcgataggg atccagagct tcaaaaatat cgaccgcttc cacactacga
    20941 gcactgccct tggcacgacc gtcttctata ttaagaacaa tgactgtctg tcccaattct
    21001 tccaataaac gaccagccac gattcctaga accccaggat tccagccttc cttggccaag
    21061 acctgaacct tcttctcagg atccaccatg gtcttggctt cttcatagat agactgaacg
    21121 atttccttgc gctcttcgtt tttctggtga atcataaggg caatctcatg cgcttcctca
    21181 tcatcaaatc cagtcaacaa atcaatggca ggattgggat catccaagcg gcccaaggca
    21241 ttcaaacgag gagcaatctg gaaaccaacc gtttcttctg ttacttcgtt agcagcaatc
    21301 ccagccatgt ccagcatttc ttgcagacca atgcgctggg tatgacccaa catttccaga
    21361 ccatattgaa ctaagatacg attttcatcc gtcagactca ccatatctgc aatagttcca
    21421 atagcgacca aatcaagcaa ttccacttgc acttcttcta acagggcaca agccaacttg
    21481 aaagcaactc cacaaccagc caaatattta aaaggataat ccgcatctgg atgttcagga
    21541 tggacaatag cataagcatc tggcagggtt tcaggcatgg aatgatggtc tgtcacaatg
    21601 acatctactc ccatagactg agccaatgca atagcctcat gaccagcaac cccattgtcc
    21661 accgtcacaa tcaaggaaat cccttcttgc tcgataaagt atttataaac actagcatta
    21721 gggccatagc catcggtaaa acgatttggc aggtaaactc ggcactcagc accaagttgt
    21781 tccaaacttt ccttcacaat agaagccgaa gtcatgccat ccgcatcata gtctccataa
    21841 acgagaatat tttccccttc ttcaatagcc tgacgaatcc gctccactgc cttgtccata
    21901 tcatggagca gataagcatc atgtaagtcc tccaaggaag gttctaaaaa cttcttcaga
    21961 ctttcttggt tctgaatccc tctctcaaac aataaccgag ccacctcagg acccagtcca
    22021 gccttcttgg ctatctttgt aaaatccgca tcttctacct gcagggcaaa ctgccattca
    22081 taagtaggtg ttatcaaaaa gacatccact ctttctaaga attctattgc ttcattataa
    22141 catgatttag acgttttctc tatccagatt gcttttttat aacattttat agtatattga
    22201 aactagaata gtacacctct acttctaaaa tattgttaga aatcgatttg actgtcccga
    22261 tcgatttttc ctgttcttat ttcactatag tgaatttttt cagatatggt ttgacaagaa
    22321 cattctttta ttgtagaatc atgtcataaa atctaacaca agggagagct ctcatggctt
    22381 tagtagaatt taaaaacgtc gaaaaatatt acggagacta ccatgcactc cgcgacatca
    22441 atctccgttt tgaaaaagga caagtcgttg tcctactcgg accatctggt tctgggaaat
    22501 ccactcttat ccgtaccatc aatggtttgg aggctgttga caaaggaagt ctcctagtca
    22561 atgggcacca agttgctggt gccagccaga aagatttggt acctcttcgc aaggaagtcg
    22621 gcatggtttt tcaacatttt aacctttatc cacacaaaac ggtgttagaa aacgtgacac
    22681 tttcgcccat taaagttcta ggaattgata aaaaagaagc tgaaaaaacc gcccaaaaat
    22741 atctggaatt tgtaaatatg tgggacaaga aagattccta tcccgccatg ctatctggtg
    22801 gacaaaaaca gcgaatcgcc atcgctcgtg gtcttgctat gcatccggaa ctcctcctct
    22861 ttgatgaacc aacatctgct cttgatcctg agactatcgg agatgttcta gcagttatgc
    22921 agaaactggc gcatgatggg atgaacatga tcatcgttac ccacgaaatg ggctttgctc
    22981 gagaggttgc ggaccgcatt atctttatgg ccgacggaga agttttagta gatatgacag
    23041 atgtcgataa cttttttgac aatccaagcg aacctcgtgc ccaacaattc ctcagcaaaa
    23101 ttatcaacca cgaaagtgac aaagtcaaat aaggaggcgc ctatgaaaaa aaattctttt
    23161 tatcagcatt attgattagc cttttcggcc ttgctgctgc caaaccagtc caggctgata
    23221 ctagtatcgc agacattcaa aaaagaggcg aactggttgt cggtgtcaaa caagacgttc
    23281 ccaattttgg ttacaaagat cccaagaccg gtacttattc tggtatcgaa accgacttgg
    23341 ccaagatggt agctgatgaa ctcaaggtca agattcgcta tgtgccggtt acagcacaaa
    23401 cccgcggccc ccttctagac aatgaacagg tcgatatgga tatcgcgacc tttaccatca
    23461 cggacgaacg caaaaaactc tacaacttta ccagtcccta ctacacagac gcttctggat
    23521 ttttggtcaa taaatctgcc aaaatcaaaa agattgagga cctaaacggc aaaaccatcg
    23581 gagtcgccca aggttctatc acccaacgcc taattactga actgggtaaa aagaaaggtc
    23641 tgaagtttaa attcgtcgaa cttggttcct acccagaatt gattacttcc cttcacgctc
    23701 atcgtatcga tgccttttcc gttgaccgct ctattctgtc tggctacact agtaaacgca
    23761 cagaactact agatgatagt ttcaagccat ctgactacgg tattgttacc aagaaatcaa
    23821 atacagagct caacgactat cttgataact tggttactaa atggagcaag gatggtagtt
    23881 tgcagaaact ttatgaccgt tacaagctca aaccatctag ccatactgca gattaaggag
    23941 gacaccccat gacagattta tcatcttgga cagcctattt tcaggatttt ggacaatttt
    24001 tcaatggttt cctctttact cttgccctag cgattggatc ctttatcctc gccatggtct
    24061 taggaatctt ctttggtgcc atgtcaacca gtaaacgtcc aattttacgc attttagctc
    24121 acatctttgt ggaattttac caaaacactc ccctcttggt gcagtttgtt attgtttttt
    24181 atggcctacc tcttatcagt gaccatacta tcatgattcc aatttattgg acagctgttc
    24241 tctgcgtggg actctatcac ggcgcttata tcgctgaggt tattcgttca gggattcagt
    24301 ctattcctag tggtcagatg gaggccgcct tgtcgcaagg ttttacctat atcagtgcca
    24361 tgcgcttgat tatcttgcct cagaccttcc gcatcattct ccctccattg accaaccaaa
    24421 ttgttaacct catcaagaac acctctactg tagctatcat ctctggagta gacttgatgt
    24481 ttgtgactaa gtcttggtcg gctctcaacg gaaactatat cccagccttt ttaggtgctg
    24541 ctcttctcta ctttgctcta tgcttccctg ttgcccagtt tggtcgcaag atggagcaag
    24601 ccaacaaaaa agcctattca ctttaggagg ttactatgga atctatttta gaagttttaa
    24661 ccccagataa cctagtcttt atctttaaag gatttggctt gaccctctat atttctctga
    24721 ttgccatcat cctctctact atcatcggta cggtgctagc tgtcatgaga aatggcaaaa
    24781 atcctgtctt acgcattatt tccagtattt atatcgagtt tgtgcgcaac gttcccaacc
    24841 ttctctggat ttttactatc tttttggtgt tcaaaatgaa atccacacca gcaggtatta
    24901 cagcctttac tctctttaca tcagcagcct tggctgagat tattcgaggc ggtctcaatg
    24961 ccgtagacaa gggacagtac gaagcaggaa tgtcacaagg cttcacctca gcccaaatcc
    25021 tctactacat cattctccca caagccatcc gcaaaatgct accagccatc atttctcagt
    25081 ttgttaccgt gattaaggat accagtctcc tctactctgt tatcgcccta caagaactct
    25141 ttggagccag ccaaattctc atgggccgtt atttcgaacc agagcaggtc ttcagtcttt
    25201 gcatcctgat tgccctcatc tacttcagct ttaacctagc aatttctagc ctgtctcata
    25261 tgctagccaa acgttggcaa caagctgcag aataatactc ttcgaaaatc tcttcaaacc
    25321 acgtcagctt caccttgccg tatatatgtt actgacttcg tcagttctat ccacaacctc
    25381 aaaacggtgt tttgagcagc ctgcggctag cttcctagtt tgctctttga ttttcattaa
    25441 gtataaacag ccactctcca gttcaattgg agagtttttt gatgagagta gatatttata
    25501 gacctcaatt tgacaccata cattttctat gataaaatgt aacaaattca tttacaacaa
    25561 atcgaaatct aaatgaaatg gaattttcta tgaaatcagt caaaggacta ctctttatca
    25621 tagctagttt tatcttgact cttttgactt ggatgaacac ttctccccaa ttcatgattc
    25681 caggactagc tttaacaagc ctatctctga cttttatcct agccactcgt ctcccactac
    25741 tagaaagctg gtttcacggt ttggagaagg tctacaccgt ccacaaattc acagcctttc
    25801 tctccatcat cctactaatc tttcataact ttagtatggg cggtttgtgg ggctctcgct
    25861 tagctgctca gtttggcaat cttgccatct atatctttgc cagcatcatc cttgtcgcct
    25921 atttaggcaa atacatccaa tacgaagctt ggcgatggat tcaccgcctg gtttacctag
    25981 cctatatttt aggactcttt cacatctaca tgataatggg caatcgtctc cttacattta
    26041 atcttctaag ttttcttgtt ggtagctatg cccttttagg cttactagct ggtttttata
    26101 tcatttttct atatcaaaag atttccttcc cctatctagg gaaaattacc catctcaaac
    26161 gcttaaatca cgatactaga gaaattcaaa tccatcttag cagacctttc aactatcaat
    26221 caggacaatt tgcctttcta aagattttcc aagaaggctt tgaaagtgct ccgcatccct
    26281 tttctatctc aggaggtcat ggtcaaactc tttactttac tgttaaaaat tcaggcgacc
    26341 ataccaagaa tatctatgat aatcttcaag ccggcagcaa agtaacccta gacagagctt
    26401 acggacacat gatcatagaa gaaggacgag aaaatcaggt ttggattgct ggaggtattg
    26461 ggatcacccc cttcatctct tacatccgtg aacatcctat tttagataaa caggttcact
    26521 tctactatag cttccgtgga gatgaaaatg cagtctacct agatttactc cgtaactatg
    26581 ctcagaaaaa tcctaatttt gaactccatc taatcgacag tacgaaagac ggctatctta
    26641 attttgaaca aaaagaagtg cccgaacatg caaccgtcta tatgtgtggt cctatttcta
    26701 tgatgaaggc acttgccaaa cagattaaga aacaaaatcc aaaaacagag cttatttacg
    26761 aaggattcaa gttcaaataa gtaatcatac tctcctggaa tttcccagga gagtttttta
    26821 tagccaaatg aaataagacc ggaacgatgg aattttggca ttcaaatcaa ttgataagaa
    26881 tattttatag tggagtgaaa taggatacga acaaattgat taggaaaatc aaatgaattt
    26941 atagaaatct tttagcagtt atgttatcct attctagttt caaaacgcta tagaagtagc
    27001 attgtgctag tctagattca gtttactata ctaaaacgag tagcttgaaa tcaaaaaacc
    27061 caccctcaca ggcaggtttt atctgtatta ttcagctaga ttatgcttta ccttctgaac
    27121 cgaatacgtc gatacgttct tcaaccgatg cttggatagc ttttacaccg tcagccaaga
    27181 atttacgtgg gtcgaagagt tttttcttat cgtattctgc ttcgtttgct tcgtagtcac
    27241 gagcaaattt acgagttgcg ttagcgaatg cgatttggca ttctgtgtta acgttaactt
    27301 tggcaacacc aagtttgata gctgcttgga tttgctcatc aggaatacct gatccaccgt
    27361 gcaatacgat tgggaatcct ggaagagctt ctgtcaattt ttgcaagtgg tcaaggtcaa
    27421 gaccttccca gtttactggg taaggaccgt ggatgttacc gataccagct gccaagaagt
    27481 cgataccagt ttcaaccatt gctttagcgt cttcgattgg agccaattca cctttaccga
    27541 tgattccgtc ttcttcacca ccgatagtac caacttcagc ttctactgag atacctttag
    27601 cgtgtgcttt ttcaacaact tctttagcca atttaaggtt ttcttcaact ggaaggtgtg
    27661 aaccgtcaaa catgattgaa gtataaccaa cttcaataca ctcaagtgca tcttcgtagt
    27721 gaccgtggtc aaggtggata gctactggta cagtgatacc cattgattca acaaggttag
    27781 cgatcaagtt gcgagcaact ttgtaaccac ccatgtattt agcagcaccc attgaagttt
    27841 ggatcaaaac tggagctttt ttagcttctg ctgcgcgcaa gatagcttga gtccactcaa
    27901 ggttgtttgt gttaaatcca ccaactgcat aaccgttgtc acgggctgct tggacaaatt
    27961 tttctgctga aacgattgcc attttatcag gcctcctgta tatttttatg ggtcatccca
    28021 tttacattgt tcattttatc actttttgcc aaaaaaatct agtttttccc gcagtttcga
    28081 ttgattttct tctaactcca tctatgtaaa ccctttctct ccctagtctt gggcgacttt
    28141 tggaaaagct ataaagaagg ttaaacgatt ctcctccatc tcgaaacgat aagctaattt
    28201 ttcatgttct aatagactct taaccacaaa gagccccata ccagacccct tgaccttgcg
    28261 actagcattg tcagaaaaag actgggctag tttttcttgt tcctctgagc tacagctatt
    28321 ttcgataaaa agttctcctt ctctttctcc aattcgaact aagccacctg gaacagagtg
    28381 cttaatggca ttgctgatga gattagaaag aatcaacttc ataactgatg ggtttagata
    28441 agcctgctga tgggtcaaac tattgtctat ctggagctct ctttccttag ctagcaaggc
    28501 ataatctttg accagatttt gcgtcatctg gaggaggtca attgtttccc tatcatctcg
    28561 caattcctgc acagaagaga gggaaagtat ctgcagaaca tggtgattga gttcatccac
    28621 aatccccaag gcaactccca gatactggtc tctatcctta taacgaccga tattctctct
    28681 catattttcg attaggattt tcaaactagc cagcggtgtt ttcaattcat gagaagctcc
    28741 tcgtaggaat tcgaccttca tcttctccag ctggagaatg gcttcattct tttcatgcaa
    28801 gtccgcaata acagtcaaga gatgctggta gaggctattg atttgttcct tgagattacc
    28861 tatctcatcc ttagaatcca cgcgcaatcg cacttgggaa tccaggtcca tcatccgacg
    28921 ggtcacccgc ttgatttcca agatcggtgc aacaatagtc cgagcgtaga tgtaggccac
    28981 caagagggaa atcagaaagg aggccagcaa ggtataggga agaaactgga gactgatttg
    29041 ctccgcttcc ttttgtaaat ccatggaagc tagaaactgg agaatcatag tagcaccgtc
    29101 ttgcgttttc acctcgcgct cctcaataaa gagagaggtt gtctggcggt ctgtgtccag
    29161 aggaagactg tccttgactt ctaacttgtc ctcggtcatc tcacctttga cggtcccctt
    29221 gatatcacta gtctgggaat acaagtctaa cacttgctcg atactctgcc tatctttccc
    29281 ttctagggac tgggcaatgg ctgttgcctt ttgaccaatg gtttcctgac gatgactcag
    29341 ataagtcgaa ggaaaaagaa aataaatagc taaatgaagg cagataacca gaacactaaa
    29401 tatcgagaag gtatagataa atatctttgc aaataaacct gttcgtttca ttttcgctcc
    29461 aatttataac caacattgcg cacagtgagg atacaatcca agtctagctt tttccgcaat
    29521 tccttgatat aaacatcaat aacacggtca aagggaacct catctgtcgc tttccagacg
    29581 gcatcgataa tctgagatcg agtcaaggcc cggccttcat ttttcactag atagtccaga
    29641 atttccaact ctttggcatt gataggcact tcttgacctg cgatgcttgc actgtagctt
    29701 tcaaagtcca ccttggtatc cttgtaagaa aagattcgtc ctgtatcgta gtagcgcttg
    29761 aaaatcgcgt ccaccctcac ttttaaaagg gagagggaga aaggtttttc cagatagcca
    29821 tctgccaaag aggcaaaggc actcatcttg tattcctcat cctgaaaagc tgtcaacatc
    29881 aagacaggaa cctgactggt tttacgaatc tcagctagga cttctaagcc gttgagcttg
    29941 ggcatctgga tatccagtaa aaccagggcc acctcatagc tagaaaattg ctccagagct
    30001 tcctgaccgt ccgctgcctc aatagtttca tagccacaat ccgtcaaata atcactgacc
    30061 ccctcacgga tcatctcttc atcttctaca attaaaattt tcatacttta actgctctct
    30121 attttttatt tttcttagaa taaataccta ctctattttc tattatagtc tcttgctggc
    30181 cttttgtctg taagcaactg accactagat aaaacgttgt gaaatccttt ctcataaatt
    30241 ccataacttt agtatattat atttaagcac taaagtacaa agaaagcaac tgaaagcaat
    30301 gattttcacc actgctttcg gatttatttt gaattgttaa atagccattc ctatccacta
    30361 ttcttgaata gaaacacaag atgcaatctt tattccagac tcattttttc aaatttattc
    30421 accatccagc aagagctctt ttggttgttt tctaaggaga ttgcttgaag caagcgccat
    30481 aacgagaacc actagaacca aggcaaggac aaaaatgatg ataaagtctg atgtctgaat
    30541 agaaatgtct aggctcgaca aggtcttgct aaagccatct acttctgcac caccaccaag
    30601 gttagaggct tgagccgcct tactagcctg tttggcaaca cctgaagtca cattggcaag
    30661 gacagtgttt ccaattgcac gggcagtgta attagctagg aagtaagcag aaactagagc
    30721 agggatagca atcaagatag attcggtgat gaattgaccc aagatacttg cctgcttgag
    30781 gccgatagag aggagaattc ccacttcctt gcgacgggcg ttgatccaaa ggctgagcaa
    30841 gagggcaagg aggagaactg agaagctcaa gctaccccag aagaggaggt tggccatctt
    30901 gtacatacca gagatagatt gctcaagagc tgggtagtta gaggagctct tgacgagtgt
    30961 gtagctcttc cagttgatac cactgatgcc attcaactct ttcataacat catccaagtt
    31021 cttgtctgct gttacaaaga aggttgcgtc cccataaatg gctgtgtctt ctgtgtatcc
    31081 ataaagtttt gcagcagtgt gaatgtctgt aatagctgtg ttttcgtaaa gttcttgtga
    31141 gtaggttact gctgacttat tatgaccatc aaagagtccc ttgattgtca cttcaactgt
    31201 ttccttggct cctttttcat tatctgcatc gtagatatta gagtccagtt taaccttgtc
    31261 ccctactttc cagccgtgtt tggctgccaa gtccttgtgc aagaggattt tatccttgtc
    31321 gtcgttggtt aagtgctctc cttcgactag tttataagaa ccagagacaa acttgtcttc
    31381 tttagaggag tcattgacac ctgtaatcat caagctactt ccaaaacgtt tggcacgatc
    31441 agcagtgaga ttcttcttgg tttctggcgt ttcaatcagg tcatatccag tcaaatctcc
    31501 gatagcgttg atacgtttga cataagactc aatggccttg ttttcggtga tttttttgat
    31561 gtcttcaccc ttgatattcc cagcaccgcg aggcgttcct tggttgacgc gacgattgat
    31621 ttgcatggag aagctattgg tgatattttt aaaggtctcc tgagaagcct tggcagtagc
    31681 tcccttgatt gacaagccga ccaaactcaa gctcgccatg aggagaataa tcaggaagat
    31741 gacaatcgat ttgaaaaact tccttgtaac ataggcaaat gcgttgtgta acatagattc
    31801 cctttctaga ttttgtttta atcattctat taaagtaagc tcaaattatt tactagtatt
    31861 gcgcgtttca gtcagtttct tatcctttaa ttcaagtgta atatctgacg cttgtgccac
    31921 ttctttactg tgagttacga caatcacaca tttacctgtt ttctgggcaa gtgatttgag
    31981 tagttcgaca atatctccag cagttttagg atccagattt cctgttggct catcagctag
    32041 aataactgga gcttctgaga ccaaactgcg agcaatggca acacgttgct gttgaccacc
    32101 tgataactgg agaacattcc gcttgatctg actttcatcc aaaccaagct caagaagtgt
    32161 atccttgctt gcctttttgt tgaccaattg gatattttcc agcggagaaa gataatctat
    32221 caagttataa ttttgaaaga ccagggaaat atggtgcatg cgatggtaag aatagccctt
    32281 cttacgaata tcctctcctt gaaaaaggat agaaccttca acaggactat ctagaccagc
    32341 aagtagggac aagagtgtgg attttcctgc tcctgactcc ccaataatac tgtaaaattt
    32401 tccgggttca aaattataat tgatctgata taggactgct tcagcagtat tcttataacg
    32461 gtaggtaaca tcttgtaatt gtaataaagt catgatttct ccttcttaac taatagatga
    32521 taaaatttct ttcggtgatt ttctaaataa gaataggaaa caaagggcta cagataagca
    32581 actaagcaga actagaaaaa cataggattc tgcaaaagat aagatactag ttgataaact
    32641 gcttgctttg gctagtgtat cttgtaagct tgcctgatct ccacttgcta gtagagtttg
    32701 gagtaggtaa gttgtgattc cgtttcctgc aacaaatgct ggaagcaaag ctccaagaga
    32761 taccaaaact acctctaaac agaattgtag gaagatcgag ctcttgcctt ttccaagtgc
    32821 aagtaaaatc cccacttcat agacccgttc tctcaaccag agagacaaaa ccagaattaa
    32881 ggctccagct cctgctatca acatcccata aaggaagatg gtcaggaagg tttggaaagt
    32941 tgcaactgag tctttgattt gttcaaaagc cttgttttcc ttttcgactt ggtagccttg
    33001 attttccaag gccaagtttt ctacctgctt catgagtccg tccatttcct taggattttc
    33061 tacatagaag cgtgctgcac tgacttgagc ttcactattg cccaaaaggg tttggctact
    33121 ttcatagtct gtaaagactt gattttcact gaagtcagaa gacaagcctg tgaatttctc
    33181 ttgtttttta ccagaaaaga tgccaataat ctcaaactct actgtttgtc cttttccaga
    33241 ttcagactga ccagcatcca agccaatctt gtcatgaagt gaaagaccgt tcttcttagc
    33301 caattcttcg tggataagga ttttcttgga atcccctttt tgaaggtgtc gcccttcttt
    33361 tagattgaaa gccgaactgg taaaggttac atccttggat gaatcctcaa gagccgttaa
    33421 gctaaccaag ttattgtctg cagctgataa atcatcacgc tccacgctct gctcgccagt
    33481 cactgcttcc ttgtctttta gttttgcgac catctcaagt tcaggagaga cattttccag
    33541 ccccttaatc ttgcttacag atgctaggtc tgacaacttg aatgtctgac cattctctat
    33601 cttcttaata gaaaaagatg tattgagtga tttataaaga ttgctttcta ctgttttgtt
    33661 ggacttcatc agagtcaaac aggctgaaat tccggccaat aagaccaata aaatcagaaa
    33721 taaaataaaa cttctcagtc gctttctgct gacataagcc caagatcttt ggattggatt
    33781 catttgtcac ctccatattt gtaagactat tataaaaccc aaatatgaaa tgtttatgaa
    33841 atacgaaaaa aaatatcgag taggggataa tctctcgccc ctctcacacc accgtacgtg
    33901 ccgttcggca tacggcggtt caactaactt ttaacgcatg tcgttcaagg taataatcca
    33961 aacacgaaac ctgttcacgt ttttcaagga ctggttttga tatagcacgt ttaagtaccg
    34021 acttctgagc tactatagta gattgaaact agaatagtac atctcttctt ctaaaacatt
    34081 gttagaaatc gatttgactt ccctgatcga ttcgtcctat tcttatttca ttttaatata
    34141 attgatagtg gtcgccccag ccagatacct tatctgctat ccatttagga acccctaact
    34201 taagcaatcc ccataatcgt ctcgatttct tcttccattg cttccagata atcactcgta
    34261 ggcgagtacg caagcgctca tctatgctag tgactatact tttcatattt ataattcatt
    34321 cctttcgttt cactcaaggc acaacacaga atgaaaaagt gttgtgatct ttattttgtt
    34381 ttataataat agtgagaaag cctatcacta ctacaaatca cgaggaggtg aataagtgag
    34441 tggtacagcc actacctcgc atattttgtc acatcattta acggtacata ataagttgta
    34501 ccatctgaat aagttgctac aatatcattt gcatgctctc cttcaccttt agcaaaggtt
    34561 ggagctcctg ctggatgatt tttatttgcc tctttcaatt tttcaataat ggcatttttt
    34621 tctgtatctt ttatattatc aggatttttc actaagattt tgtctggata tgtcggttta
    34681 gcagaaacaa tttttactgt tatttctttt ttattcgaag cacttgtcca gtttccagca
    34741 ttatctttag tatttaattt tacagtaatt cctgaactag gaacttcagt agcaggttga
    34801 ttatcaacat tattcaactt taatttcaaa agagctgttg catcagacgt tttatcaatc
    34861 gttatatata atgataaatt gttattataa ccagtttctt catatttagc tgtttgtgag
    34921 ctacttgaaa cagaactgaa attatgccca ctacctccct gattatcttc aatgcttacg
    34981 tctaaatgaa cttccccact attatttggc ttagcaacaa ctgttatagt aaaataacat
    35041 aaaatttgca taaatagatt agggaaatca aagcaacttc taggaatgtt ttagcagtca
    35101 cagtgtactt tcccagcatc aatccactat aactctgcac ataaaaatgg agaagatggc
    35161 catcctcttc tccaaatatt aacttcttta caaaccaact atagttgaca aagaacctaa
    35221 aatcaattga taacacaagg tcaggtcggt caactctttc aactgaagcc ctgtcaactc
    35281 ttcccattta tcaatcttgt attggagaga attgcggtgc agatagagtt gctgggctgt
    35341 tttagtgaga acagcactat tttcccaaag agagagaatg atttcctgaa tctgatcttg
    35401 atccaaaatc atctggtgta gacattcttt aattggcttc aagtccacga gtctttctcc
    35461 catactccaa agatagagct gagaaaaagt atgaacacct tggtgaccct gacgccacca
    35521 tgtcttgaac aaatcccgct cagctttgat taagtctgat agggcttgat gtcctgtctg
    35581 agaccaaacc tgacccaaca tgatagaaag acgaagtcca aagtcatact caaccgcttc
    35641 aatcgtatca cttaaaatat ctcttacaga agtgtatttg tcttgttgaa gcacgaaaac
    35701 ataatcctga gatccgacct gtagcactgt ctgacaattc ggaaaaagag tccgcatcat
    35761 atctagccaa gaagccagat tttcctgctg aaaataagaa agatggcaat aaaccaactg
    35821 aatcttttta aaaacttgcg gtgcctgtcc cttgccttca accagatagg aataccaagg
    35881 gtttagcgaa cgagcctgct cctgctgggt caaaagggca accaactgct tttcacgctc
    35941 gctgagccca gcttcctcca gcaaaatcca ctgctgagaa gctaaaggga gcgtgagata
    36001 gccctctttc tctactggtt ggtctgaaat ccgagcctca ggaaaccagt cttgtagttc
    36061 tttttccctc atgttctagc cctccacttt ttggatgcac catgaaacca aactctcaag
    36121 acgttccaga ttctcagtca tatggagata gcccataacc gcttcaaatc ccgtggacat
    36181 acgataagtc acgacatctg catttttagc ctttgtgtgg ctattggtat tgcggccacg
    36241 tttgtagatt tcttcttctt tttccgttag gacctgctcc tccaacatga gagcaatcag
    36301 gcgagcctga gccttggctg acacatactt ggttgcttct tgatggagtt tattgggttt
    36361 ggtcatacct ttgaggatga ggtgacggcg aatatacata gaatacaccg catccccctc
    36421 aaaggctagc gcaatcccgt taatgagatt gacatcaatc acgtgtccac ctcactccat
    36481 ccttggtatc aaggagctta attccttgag taaccaattg gtcacggatt tggtctgctg
    36541 tctcaaagtc tcgattggca cgcgcctctt ggcgtttttg aatcaagtct tcaatctctg
    36601 catccaaaac ttcctcaaca aagacaattc caaaaatttc taacatatct gcaagagctt
    36661 gcttgacact tgcatcatag ttccctgagt tgatccattt ggccatttca aagacaactg
    36721 tgataccgtt ggcagcatta aaatcttcat ccatagctgc tacaaactta tctttaaagt
    36781 tttgtaactc ttgggcatcc acgtttcctg taaatggttg ttcgtaagta ttcttcagat
    36841 acttgagatt ggtctcggca tcgcgaactg ccttttccgt gaagttgata ggcttacggt
    36901 agtgctgggt cgcaaagaag aaacgaagta cttgcccatc aagagtttta agggcatcgt
    36961 gtaccgtaat gaagttaccc aaggacttag acattttgac attgtcgata ttgacaaagc
    37021 cattgtgcat ccagtagtta gcaaaagcct tgcctgtttt agcttcagat tgggcaattt
    37081 cattggtgtg gtgtggaaac tctaggtcag ctccaccacc gtggatatca atggtatcac
    37141 ctaaaatctc tgtcgacatg actgaacact caatatgcca acccggacgt ccaggtcccc
    37201 aaggactatc ccaagaaatc tcacctggtt tggaagattt ccatagagca aaatctacag
    37261 gattttcctt acgagccgtt tcttcatcgg tacgacctga agcacctagc tccaaatctt
    37321 ccaaggtttt attagccaat ttagcatagt tgtgggattt ttctacacgg aaatagacat
    37381 ccccttgact ctcataggca aagcctttct cgatcaagtc ttccacaaaa cggatgatgt
    37441 ctgccataaa ctccactaca cgcggatggc gagtcgcagg tttcacgcct aaggccgtca
    37501 catcctcacg aaaggcagcg atgtacttat ccgcaacctc ctgaggtgtg atgccttctt
    37561 ccctggcacg gttgataatc ttatcatcca catctgtaaa attggaaata taggcaacct
    37621 tatacccacg gtactcaaaa tagcgacgaa tcgtatcaaa agctaccgtc gaacgggcgt
    37681 ttcccacgtg aatatagttg tacacggttg gcccacaaac atacatcttg atcttgccgt
    37741 cctcaatcgg gacaaattct cgcaaatcac gagacatggt gtcatagatt ttaatcataa
    37801 atcataatca agaaagctaa aatccaagaa ctgttagttt catcactaaa ctaagagctc
    37861 aagtaaattt cagtccgaat gtctctacac ttcggaatcc cttgctcctt tctcattcag
    37921 ataaaccacc tgtgtctgtt tgacaaagcc aattttttca tacaaacgtt tggcacctac
    37981 attgctatct tccactgcaa tctgaaattc cttatcattt tgctcaatta gttggttgac
    38041 aagggatttt gctaagtagc ttccatagcc ttttccacgt tcaggttctg atattgctaa
    38101 accgtagaag taattcgtat cgctcgataa atcaaccgtg caagtcccaa taacctgacc
    38161 gtcttttaac aaaatatata agcgactttc tggatccttc agagcttcag cgacatatct
    38221 atccacaact tctctcgatt catgttcccc tgaaaatgcc tgaaatttta actggctaat
    38281 ttgctcctga tacgaactat ctgctaacaa aacttcaaga ttagaaagtt ttgctaactg
    38341 atatggtctt ctatccttac ctaaccaggt ttctgtctct tcatcctcga ttagtcccca
    38401 gttactggca aagtcaggat gattctctaa aaaaatacgt tctgtctgaa aagtgactga
    38461 ccgaatgggg aaagaagctg tttctctctc aaaactagta aacaatgcac gcgcaatccc
    38521 ctgacggcga tgacctggat gaaccagtat cgtcacttct acatcttggt catctgcata
    38581 gacagttaat aaaccaacaa gtttgccttt ttcataataa aggaaaaagg cgggcatgtt
    38641 tgggtcaaaa ttaagcatgt tagagagata gggatcgcga taggtaccgt catagttttg
    38701 gcaacagtta attacttttt tcgcctcaga tagctcctct tggcttaact tgtttcttgc
    38761 ttgaatcata taggtatcct ctacaaacca gacgatctgt gactggcatc tttagcctgc
    38821 tcgagtttat tgacataata ctcccgtttt tcttcgactt cgtgaataac aggctcatct
    38881 ttcttaccat gaagacggac aatcttggcc ggaataccga caaccgtcac atcactaggt
    38941 acatctgcta cgacaactgc tgcagcaccg accttggcat tttcaccaat ttccacaggc
    39001 ccgataactt gggcatgggc tgatatgagg gctccctttc gtacagtcgg atggcgtttg
    39061 ccacagtctt tccctgttcc cccgagagtc actccgtgat agagaagaac gcctttttca
    39121 acaatcgctg tctctccaat caccagacca gaaccatggt caataaagac acctgaatca
    39181 atctgggctc ctggatgaat ctcaatctga gtccaaaagc gccaaaactg actgtgcata
    39241 cgagctaaga gtttgaagtc gtacttccag agaaaatgcg agagacggtg ggccgccaag
    39301 gccttgacac ctggataagt cagcaaaacc tccaaagtgg tgcgggccgc tggatcattt
    39361 tcttttacga tatcaatggt ttcgtgccac caccccatac atttctcctt ttcttactct
    39421 gaatcttttg gtgtttctgt aaattctttc ttaggtttgt gatccttgtg atgacgtggg
    39481 cggtgagggc gctcagactt ttcacctttt tcatcatgct caggttttgg cggacgaggt
    39541 agaagagcct tcatagaggc atcgatacgg cctttttcat caatcttgat aaccttaaca
    39601 tcaacttcat ccccgatttc taccaaatcc tctacacgat tggtacgagt ccaagccatc
    39661 tcagagatat gaacaagggc atctgtctta tcaaagaggt taacaaaggc accaaatttc
    39721 tcgatacgaa cgactttagc acggtaaact tcatccactt tggcttcacg aaccaaacca
    39781 gcaataattt ctttggcacg gttaatagca tcttggtcac tagagtagat agacacattt
    39841 ccttcttcgt ctatatcaat cttaacacct gtttcagcga taatcttgtc gatggtttct
    39901 ccacccttac cgatgacaat cttaatcttg tccacatcaa tcttgatcgt atcaattttc
    39961 ggagcagttg gagccaattc tggacgaact tctggaatgg ttgcttcaat gacatcaagg
    40021 atttcaaaac gcgctttctt ggcttgagca agagcctccg tcaagatttc tgcagtaatc
    40081 ccttgaatct tgatatccat ttgaagggct gtaatcccat cacgagtacc tgcaaccttg
    40141 aagtccatat ctccaaagtg atcttccaaa ccttggatat ctgtcaatac tgtgtagtta
    40201 tttccatctg agataagtcc catagcaata ccagctactg gcgccttgat tggcacacca
    40261 ccagtcataa gggcaagagt tcccgcacag atagaagctt gagatgaaga accgtttgat
    40321 tccaaaactt ctgctactag acggatagcg tatgggaatt cttccaagct tggcaagact
    40381 tgagcaagag cacgctcacc aagggcaccg tgaccgattt cacgacgacc tggcgcaccg
    40441 taacgacctg tttcccctac agaatattga gggaagttat agtggtgcat aaagcgtttc
    40501 ttgtactctg gatccaaacc atcaatgatt tgagtttctc ccatcggagc caaggtcaag
    40561 actgaaagag cttgagtttg cccacgagta aagagacctg aaccatgtac acgaggaagg
    40621 aagtcaacaa ccgcatccaa aggacggatt tcatcgacct tacgaccatc aggacgcacc
    40681 ttgtcttctg taattaaacg tcgcacttct gcgtgttcca tttgttccaa gatttcagcc
    40741 acatcacgca taatacggtc aaattcttcg tggttcgcat atttttcttc gtaaacggca
    40801 gtcacttggt ctttcactac ttgagttgca gcttcacggg ccaatttctc ttctacttga
    40861 actgcctttt ggaggtcact gttgtaggct gcaatgattt cagcttgcaa ttcagcatcc
    40921 acgtgaagca attccacttc tgctttttct ttaccgacag cagcaacgat ttcttcttgg
    40981 aaggcaatca attctttgac agcttcgtgc cctttaagaa gcgcttccaa catgatttct
    41041 tctgacaatt ctttggcacc agactctacc atgttgatag cgtgcttggt tccagctact
    41101 gtcaattcaa gaagagattg ctctgcttgt tcttgacttg ggttgatgat gatttggcca
    41161 tctacatatc ccacttgtac cccagcaatt ggtccgtcaa atggaatatc tgaaatagac
    41221 agtgccaaag atgaaccaaa catagcagcc attggtgcag atgcattttc atcataagaa
    41281 agcactgtat tgatgacttg gacttcatta cggaaacctt ccgcaaacat aggacgaatc
    41341 ggacggtcaa tcaaacgcgc tgtcaaggtc gcatctgttg aaggacgtcc ttcacgtttc
    41401 ataaagccac caggaaactt cccagccgca tacatttttt cttcgtagtt gacttggagt
    41461 gggaagaaat ccccagttgc cattttctta gacataacgg cagcagtcaa gacagttgac
    41521 tcaccgtaac gtacgacaac agagccattt gcttgcttag caacctgacc agtttctaca
    41581 attaactcac gacccgcaaa agtcgtttga aacacttgtt ttgccatttt aatccccttt
    41641 ggattgatga aattatacgc cttgcctaca aagatcaaga taccaaggac gtcaaaagca
    41701 aagtaaaaat aggaaactga cgaagtcttc gatgaagaca agacagttta tcttttttac
    41761 acagcttttc ggccgtgttc aattacacaa gatattttgg acggttcggc ttgccgaaca
    41821 tttctgtaga aaaataggaa ggtgacgtcg cactcgacga gtgctaggaa gcttatcttt
    41881 tttcctaaga aatgagacca aaattcaatt actcaagata ctaagccgtc aagcaactca
    41941 aaggaaaata ggaaatcgaa cgacggagcg actgctccta gggagattta tctttttcca
    42001 cagagttgta ggcgagttca gttttcaaga tataaatcat tagaaaggtt taatactaaa
    42061 gtatctaaag ctttcacgct aatcgctatc gggcgattag ttaaatgctt tactaactct
    42121 ctcgtcaaat aacatcgatt tgactcactc gtgtcgttaa atcttacagt ttaaatgcat
    42181 tgtattattt aataccttca tctttgtatc aagtacgtac agaatttatt ttatcatatt
    42241 tttcttaaaa agtgaggtct ttaccattaa aaaggaacca ttcccctcac ctgagaagaa
    42301 tggtttgctt ttattatcct agagactggt gattaaacaa ggcatgggtt gcttgatgga
    42361 tgtattttgc tgtatcagca ttattcatcg tatagagatg cacaccggca acatcctgag
    42421 ttaccaagtc cacgatttgg tccactgcat aggcaagtcc tgctgctctg agcgactcag
    42481 ggtcatgctc atacttgtct aagatggctt taaatttgcg tggaagatgg atattctcac
    42541 aagtcttcaa gagtcggaga gcctgatttc gattcagaat tggcataatt cctgcatgaa
    42601 tgggaacatc aatcccagcc aagatgcact tgtcctgaaa atcatagaag cgctcattgt
    42661 caaagaagag ctgagttacg aggctcgaac agcctgcatc cactttcttc ttaagatttt
    42721 gaatatctga aatctgattt ggcgaatctg gatgcccttc tggatagcaa gctccaataa
    42781 tatcaaagtg aggggtttgt tccttgataa actcaatcaa gtctgttgca tagcggaaat
    42841 ccttttgtgg ttccacgtct ggaataatat ccccacgaag agccaagatt ttctgcaccc
    42901 caactttgtc caagtcagca atagtttcag caaccttgtc cttagttaga taaatagctg
    42961 gcaagtgggc aatggtcgga atcgccaaat cattttggat aaagtcagcc aaacgaaccg
    43021 tcgtttcctt gatattaaat ttattattgc tggcagttac actgataaaa tggggagcca
    43081 actcctgcat atcctgcaag gctgaaataa tgttatcatt acccacggct gggtttggag
    43141 ggaacacttc aaatgagagt gacggtgttt ggcgtgacat atgtaataac cttttctagt
    43201 tgatttcttt ttgaacaacc actgtatgga gagaaatcca atcttacaat ttctcacgcg
    43261 cagctttagc tgcttcaaca aggcggatca agctttcttt tgtttctggg ataccacgtg
    43321 ttttcaaacc acagtcaggg ttgatccaaa ctttcttgct tggcacttta gcaaggatga
    43381 cttcgattgt gttgtcgatt tcgccttcat ttggtacacg aggtgagtgg atatcgtaaa
    43441 ccccaggtcc cacttctgtt tggaagtttt tcgctttgag ttcgtccaag atttcaaggt
    43501 ttgaacggct agcttcaaag gaaataacgt ctgcatccat gttatcgata gctgggatga
    43561 tatctgtaaa ttctgagtaa cacatgtgag tgtggatttg tgtgtctggc gctactgttg
    43621 agtgtaccaa gcggaaggca ggaatagccc agtcaaggta gtcttcgtac cagtcgctac
    43681 ggcggagtgg caatttttca cgaagagcag cctcgtcgat ttggatgatt ttcacaccag
    43741 cagcttcaag gtcaagtact tcatccttga tagcaagggc gatttggaga gttgaatcct
    43801 tgatagagat gtcttcacgt gggaatgacc agttaaggat ggtaacaggt ccagtcaaca
    43861 tacctttaac aggtttgttt gtacgacttt gtgcatagct agaccattta acagtgatag
    43921 ggttaagacg agtgacatca ccccagatga ttggtggttt taccccacgc ataccgtatg
    43981 attgtaccca tccattttta gagaagaggt atcctgacaa gttttgaccg aagtactcaa
    44041 ccatgtcatt acgctcaaat tcaccgtgaa caaggacatc aaagtcaata tcttcttgcc
    44101 acttgatcca ttcgtcaatc gtttcagcaa ggaaagcgtc gtactctttt tgagacaatt
    44161 cacctttacg gtaagccaaa cgtttggcac gaacttcttt tgtttgaggg aatgaaccaa
    44221 tcgttgttgt tggaagagct ggaagtttga aagcttcttc ttggatagct tcacgttctg
    44281 caaaggctgg caaacgagtg tagtctgcgt ctgtcaagcc agcgatacgc gcacgaagtt
    44341 cagcattttc accaacacgc tcagtcgcaa agagttcttt gttagctgca agtgcttctt
    44401 ctccttgacc attgcggata gcatccaaat cacgaatctc atccaatttt tcaactgcaa
    44461 aggcaaagtg gttcaagatt gctggttcaa attcttcatt agctgttgta aatggcacat
    44521 gaagaagtga gcatgagctt gtcaagacaa tgttttcagc tgggatttgt tcaagaacag
    44581 ccaagctctt ttcgtagttg ttacgccaga tgtttttacc attgacaata cctgcgtaga
    44641 gagtcttgtc agctgggaag ccacctttaa ccaattcaag agttttctta ccttcaacga
    44701 agtcaagacc gatagcatct actggcaatt tcacaaggtc agcgtaaacg tcacgaacgt
    44761 caccgaagta agtttgaagc aatacttcaa gacctttttt gtcagccaaa agtttattgt
    44821 aaaggttcaa gaagagagct ttttcttcgg cagtcaaatc tttcacaaga gcagcttcgt
    44881 cgagttggat acgagatgca ccaagttcag ccaatttagc aaaaacttct tggtaagcag
    44941 ccactagact gtctacgaaa tcttctgctt tcacaccttc ttcaaagtct gacaattgaa
    45001 ggaaagtgaa tggacctaca aggacaggac gagtgttcaa tccaagttct tttgcttctt
    45061 ggaattcatc gaaaatcttg tgatctgcca attttacttg agtgtctttt tcaaatttag
    45121 gaacgatgta atggtagttg gtgttgaacc atttcttcat tggaagggcg cgaacgtccc
    45181 ctttttctcc ttggtaacca cgacccaaag cgaagtagcg ctcaaggtca gacaagtcca
    45241 agttttgaac tgaagcaggt accacgttga aaaggaaagc tgcatctagg aagttgtcat
    45301 agtgagaaaa gtcatttgat ggaatttcag tgatgccttt ttctttgaca atgttccagt
    45361 gtttagcacg caagtctttt gctgctgcta aaagttcttc ttctgagatt tcttttctaa
    45421 agtatttttc agttgtaaat tttaattcac ggaattcgcc caaacgaggg aaaccgatga
    45481 ttgtagttga catgatgtgt cctccaaaat ttgttgttga aactatctta acagaaaaga
    45541 aagcgtctgt ataattgtaa aaaattaggg tttgatatag tttgaaacta tatatctgtt
    45601 tcggacaaaa gaaaaagact tgaagcaaac gtctcaaatc ctttgtaatt cttactttac
    45661 agctatattc caattagaat actaaaacat gttattagta attcttataa gtgactatga
    45721 cctgttatta gaaaagacta taactgattc tagtcaactt tttccctgtt caagtgggac
    45781 gactgctagt gtctttccta aactggctag gacttttaag actgtatcca actgaggact
    45841 agtctttcct gtctccatcc tagctatgac aggctggctt attccactga cttcttccag
    45901 ctttttctga ctgattcctt gttcatacct agcctcaatc aactcgctca tgatagccac
    45961 tcgcatatca ctttcaagga tttcctcctt gctaaagagc tcagatggac atccttccaa
    46021 ttactcccaa tagcactatt cttcatcact taaccctctt ttttttacgt ctatgtattt
    46081 ttaaaaaaat gagcgaatta tgattcgata gattgaccag tgggtttaaa gttggtgcta
    46141 gcctatttct taagcgattt tccttttcta ggataaagca gatcctgctt gcttaacccc
    46201 aattttccac gatgaatcca atagtaaatg gttgaaattc ccacgttaac ccctttaacc
    46261 ataaccatca tttcaggcga aaatttttgg ttatgtatag tggagaatct tttcctttag
    46321 tttcttaaga ctgttgagcg tagtcggcag aataaacctc tttgaagcgc ccttttccaa
    46381 gacattgtcg gactgtccca cgcttgattt cagtgtggat agtttgagga acttttccaa
    46441 gcagagaggc aatttctcta tttgatttcc cttctttttt ccatctttcg attaagcgac
    46501 ggctatcgat tgtcaaatgt ttgccttttg tagtataatt gtcttgcatt tctgtgcctt
    46561 ttaatcattt caatcttaaa ttggactttt tttacttggg ttgtacttaa tctatgagga
    46621 agacaagaaa aagaatatca atcaagtaaa gtcacaaagt cacattagct ccgagcaacc
    46681 attgcaaatt gaggtactca cacaatgatt aaaacatttc tctctgccct ttcggtcatt
    46741 ctctttgtta tccctatcat aacttattct ttttttccat cttctaatct taatatttgg
    46801 ctatctacca gacctatctt ggcacagatt tatgccttcc ccttagctac tgcaactatg
    46861 gctgctattt taagtttcgt atttttttcc ctatcttttt acaagaaaaa taaacaaata
    46921 cggttttact ctggcatttt gctcttacta tcgctcatat tactattatt cggaacagat
    46981 aaaacccttt cttctgcatc aaataagact aaaaccttaa aattagtaac ttggaacgtc
    47041 gctaatcaaa tagaagcaca acatattgag cgaattttta gccattttga cgccgatatg
    47101 gctatattcc ctgaactagc taccaatatc agaggtgagc aagaaaacca gagaatcaaa
    47161 ctattgtttc atcaagttgg actttctatg gccaactatg atattttcac ttctccacct
    47221 accaatagtg gaatagctcc tgtgactgtg attgtcaaga aaagttatgg tttctataca
    47281 gaagctaaaa cttttcatac aacacggttc gggacaattg tattacattc gagaaaacaa
    47341 aatataccag atatcattgc cttgcatact gctcctcctc tgccaggttt aatggaaatc
    47401 tggaagcaag acttaaacat cattcataat caattggctt caaaatatcc aaaggctatt
    47461 attgcaggtg attttaatgc aactatgcgt catggagcac ttgcaaaaat aagctctcat
    47521 agggacgcat taaatgtact accacctttt gaaagaggaa cttggaatag ccaaagtcca
    47581 aaacttttta atgcaacaat agatcatatt ttattgccta aaaaccacta ctatgttaaa
    47641 gatttagaca ttgtaagttt tcaaaactct gatcatagat gtatttttac agaaatcaca
    47701 ttttaattat tttatactct tcgacaatct cttcaaacca cgtcagcctc gccttacagt
    47761 actcaagtac agcttacggc tagcttccta gtttgctttt tgattttcat tgagtattat
    47821 ataaaatcac ccctctagtg ttcataaact agagggggaa tttgtatcct actatcgttt
    47881 aacgcacttc tgcgttcact tttttttcaa gcgatgcttg gattttttcc atgtagcgtg
    47941 cgacttcttc gtccgttaag ctgtcttctg gattttggaa ggtcaagcta taagccattg
    48001 acttcatacc aagtcccaat ttctcacctg agaaaacgtc aaagagtttg atatctgtca
    48061 aacgtttcac gccggcagct tggatagcat ctataacttc ttggtgagtt actgctgcct
    48121 tgaggagaag ggaaacgtca cggctgactg ctgggaattt cgtgatttcc acaaatggag
    48181 cagcaggttg aagggcagct tcgatgactg aaaggttgag ctcagccaca tacgtttctg
    48241 gaatatcgta agccttagca gtgactggat gcacttggcc aagaaaacca agaacttggt
    48301 caccgagtga aatgactgct gtacgtcctg ggtgaaggct ggcaatctca gctgttgctg
    48361 tataggtcac ttctagtccc aagcgagtaa agagcgcttc taggattcct ttagcataga
    48421 agaaatcaac tggaactgct gctgtttgga aatctttttc agcaaccaag cctgtcaagg
    48481 caaaggcaaa actgttgatt tcatttggaa gttcttcttt tggattacct gtttgttcaa
    48541 agacttttcc aatctcgtaa agggccaagt ttttattctt acgagccacg ttgtaggcaa
    48601 cggtatcaag gatacctgaa atcatatttt gacggaggac tgaacgatcc acagtcattg
    48661 gccacatgag ttccgtaagg ttacttggtt gagccgtaaa ctcaactgct ttttcaggag
    48721 ttgttagagc ataggtgatg atttctgtca aacctgctcc ttcagcaatg gtacgaactt
    48781 gacggcggag tttttgtgtc acagtcaatt caccagctgt accatcgtct tttggaaggc
    48841 tggttggcaa gcggtcatat ccatagatac gagcgatttc ttcaaagaga tcagcttcga
    48901 ttgtgatatc ccaacgacga cgtggtacgc tgactgtaaa gctgtctgca tttccagaaa
    48961 gaccaaagcc aagacgacgg aagacgtctt cgacatcagc ataagacagc tcagttccga
    49021 ggacacggtt aacatcagca agagttgaag aaacttctac atctgaagta tcaagctcac
    49081 ccgctgaaac gataccctta cgcaccgtcg cacctgcaag ttccgcaatc aggctagctg
    49141 ccgcatcaag ggcttcatta actgttgcca cattaattcc tttttcaaag cgagaagatg
    49201 actcagaacg aaggttcagg cgaccacttg tcttacggat agatttgcca ttgaaaacag
    49261 cagcttcaag gacaacatga ctagattttt cagagatttc tgttgcttga ccacccatga
    49321 cacctgcaag ggctactggc ttgtctgcaa cagtgatgac taggtcattc acgtccaagt
    49381 cacgttcttc accgtccaag gtcaccaatt tttcaccagc acgcgcttca cgcacacgga
    49441 tgtcagtccc ttcaaatgtg tccaagtcaa aagcatgcat aggttgacca aagtagagca
    49501 ggatgtagtt tgtcacgtct acaacgttat tgatgggacg gatgccttcg ttcatgagaa
    49561 ggttttgcaa ccattgtgga cttggtgcga tagtcacatt gtccaagata cgagctgcat
    49621 agtaaggcgc cttgtctgtc tcaatgccca cagaaagggc atctgtcgca gcttcatttg
    49681 tttctgtcaa agtaaattct ttaaagttga ctgccttgtc atatatggct gccacttcgt
    49741 gagccactcc acgcatagaa agggcatctg cacggtttgg tgtgatagaa aattcgatga
    49801 tttcatcatc caagtctaga tatgaaaaga cttcctcacc tggaacggca ttttctggca
    49861 aaatttgaat accatctgcg aatttcttag gtacaactga gtcagaaata cccaattcac
    49921 caagtgaaca gatcattcca agtgactcta agccacggat ttttcctttt ttgattttgt
    49981 aattgtcagc gatacgagct cccggaagag ccaccataac cttgatgcct gcacgcacat
    50041 ttggggcacc acaaacgatc tgacgctctt cttcttcgcc aacgttaacc tgacaaacat
    50101 gaaggtgagt ttctggcaca tcttcgcagg acaagatctc accgacgaca atttttgaaa
    50161 gaccggcagc tggtgattcg acaccttcga cctcgatccc tgtggttgac attttttcag
    50221 ccaactcctg tgatggcaca tcaatgccca ccaattcttt taaccattta taagatacaa
    50281 gcataattta gttctccaga atgaccattg ccacactggt tcttttcctt tcctatcatt
    50341 tcaatagaag aattctcttc ttaccttaat ttctttctca gtaaccaatc cgtatctact
    50401 ttttgaccaa ccataaaatg atgttggcta aatttttcaa aaccatatcg gttataaaac
    50461 gcttgagctt ttgtattatg ctcccaaaca cctagccaag cccaagaaaa actatttttt
    50521 gtagcaagtt caagtgcgaa ttcaaacagt tgcttaccta gtccaaatcc ttggaatttt
    50581 tgtagcacat agaggcgttg aatttcaaaa gcgtcctcta attctctctc agtttgagca
    50641 cttccccagt tgactttgag aaaaccagct atctcctcct catgcataat gaaataggtt
    50701 tcagagtcag gatttcccaa ctcagttgac aaagttttca gactataagc ctcttcaaag
    50761 tattcctgta actgctcttc cgtattatca tacgcaaagg tttcacgaaa ggcttgtttg
    50821 gcaattttag ccaacacctc aacatctgcc atttctactt ttctaatcat tatttaaact
    50881 gttctgagaa gcggacatct ccttggtaga atccacggat atcgttgatt ccataacgga
    50941 gcatagctac acgctcttgt ccaagaccaa aggcaaagcc agagtataca gtcgcatcga
    51001 taccactcat ttcaaggaca cgtgggtgaa ccataccggc ccccataatt tcgatccaac
    51061 ctgttttctt acatacgtta cagccttctc caccacattt gaagcaagaa acatccacct
    51121 caacagatgg ctctgtgaat ggaaagtaag atggacgcaa acgaatttga cgctcttcac
    51181 caaacatttt ttggacaatc aactgaagcg ttccttgaag atcagccata gagatatttt
    51241 tcccaactac caagccttcg atttggtgga attggtgact gtgggtcgca tcgtccgtat
    51301 cgcgacggaa gacacgccct ggcgagatca tcttcaaagg acctttagaa aaatcatggg
    51361 catccatagc acgcgcctga actggagacg tgtgggtacg gagcaagatt tcttcagtga
    51421 tatagaaagt atcctgcata tcacgagctg ggtggtcttt tggaaggttc atacgttcaa
    51481 agttatagta gtcttgctcc acttcaaaac catccacgac ttgataaccc ataccgatga
    51541 agatatcttc gatttcttca ctggtttgtg tcaaaacgtg atggtgacca gtcgcaactg
    51601 gacgacctgg aagcgtcaca tcgatactct cgctagccag ttgagccgcg actttctttt
    51661 cttccaagag cttagctgtt tcttcaaaag cagcagtcaa gacatcacga gcttcattga
    51721 cgtgtttccc gatgattgga cgcatctcag cagaaacatc tttcatccct ttgaggattt
    51781 cagtgagcga acccttttta ccaaggacag agacacgcaa atcttgcatc tctttttcat
    51841 ttccagcagt aatctgcttc aagctagcca gcgtttcttc gcgaagcgct tttaattgtt
    51901 cttcaatagt tgacatattt cctccatcag tctctcgtag ataaaaagaa aaccacatgc
    51961 caaaaactcc actcggagcg ttgacacgcg gtaccatccg ttttcatctg acaagtcaga
    52021 ccttcatttc taaatccatg cgcaagtgaa ttcacccagc tttcatatag agagcttgca
    52081 gtcacggctc tcctccctga tatacttccc ttgggctact agtctttcag attcctattc
    52141 aattactact tagtttatca gatttttacc attcttgcaa gacctatctt acttctgctt
    52201 gttagcttat tcttatctaa ctttatagcg gattgaaata agatgaaaac aaatcgattg
    52261 ggaaagtaaa attaatttct ataaatgttt tagcaattgt ttcgtactat tttagattca
    52321 gtctactata tataacccat ctatcctaag ttaactattt ataatcggct ctctgtaaca
    52381 aaattaaatt aattaatccc cctataaaat aaagaggttt agaatttgat gtcttccaaa
    52441 cctctttatt tcatatttaa tgagatgttg ccttaaccgt gataatatct aatcatcaat
    52501 aaacaaacta tttgaataag gattctccat ttgattcaat cactccttta taccaagtaa
    52561 aggacatttt cttatatcga ttgaatgtgc catttccatc atcatttcga tcaacataaa
    52621 tgagaccgta ccttttagaa agttgtgcag tggacataga aacgcagtca atacatcccc
    52681 aagatgtata gcccataatt tcaacaccat cctgtagagc ttcagcaact tgcaataaat
    52741 gttctttcat atactgaatt ctataatcat cttggacggt taagttatta agttcatctt
    52801 ttattagttg atctttagca cctaatccat tttctactat aaataatggg atttgataac
    52861 ggtcataata tctatttaaa attatacgta gtccaattgg atcaatttgc catccccact
    52921 ctgaagactc taaataagga tttactaaac caccaataat attcccttct cctgaattat
    52981 actgtgttgg aagagcagat tgagtcacac tcatgtaata gctaaaggat aaaaaatcta
    53041 cggtataatt ttttaataac tctgcatctt cagctgcaaa ctctatgtta atgtcatgtt
    53101 ccttaaaata tctttttgca taattcggat aataacctct aacatgcaca tctgaaaata
    53161 gataatttag attctcatac tcatgagtcg cccatacatc ttttggattt ggagtcattg
    53221 gataagctgg catagctaat accatacacc ccaccttaaa ctctgaatta atctcacgag
    53281 caatttttgt aaccaaactt gaggcgacta attcatgatg tatagcttga tataattctt
    53341 gtttcgaaag attctcctta ggtatatcta ttcctccact agtaaatggt aattccaaaa
    53401 cagagtttac ttcgttaaat gtaagccaat atttaacttt atctttatac ctttctaaaa
    53461 ctgttcgagc aaatttttca taaaaatgaa tcattctcct atcaacccat ccatgatatt
    53521 ttcttgctaa atataatgga gtctcatagt gtgaaagagt tacaagtggc tctatcccgt
    53581 gagcatgtag ttcatcaaac aattcatcat aatatttcaa accagcttcg ttaggttctt
    53641 cttcatctcc ttttggaaaa attctactcc atgcaataga agtacgaaaa acattaaagc
    53701 ccatttcaga aaacaaggat atatcttcct tatatttatg ataaaaatca atacctatca
    53761 attttaagtt atcttctgta ggattttctg ttgcttctcc taatccacct ttgggtaaca
    53821 catcctgaac tgataagccc ttaccatctt cattatatgc tccctctact tgattagctg
    53881 caacagctcc accccaaaga aaatcatctg gaaaaatggt cataactttc ctccattata
    53941 atattaccag taattcctta gaatgctcga ttgtctgatt attaggtaat actaatacat
    54001 ctagaaaatc attggtattc gttacaatta ctggtgtaac tgtttcgtag cctttagtct
    54061 tgattaaatt caagtccatt tcaaaaatca actgattttt gaaaactctg tctccttctt
    54121 ctacatgact aataaaacct tgacctttta gctcaacagt atctaatcca atatgaatta
    54181 gtaactcaac accctcatca ctcttcaatc caattgcgtg cttagtcgga aaaatatttg
    54241 taattttccc atcaaatggt gcataaacct taccttcact tgggataatc gctactccgt
    54301 ctccaattag tttatctgaa aatgttttat cctggacatc gcttaacgga atgatttctc
    54361 ctgatatagg agaaaatatc atttttttat ttgaaactcc agctttaact tctaaattac
    54421 tagaactctc ttcttcatcg attccaaata tataagctaa tacaaaggta ataacaaccg
    54481 aaatgaccgc cacaattaaa gcatttacaa tatttgatgg tacatcagaa taaataaatt
    54541 gaggcaacgc tatcaaagat gggacagcaa atagatacgc tttaacacta gtaagacctg
    54601 caaataatcc cgctaatcca ccaccaatca tagctgcata aagcggtttt ttatatttta
    54661 aagtcacacc atataatgca ggttcggtaa ttcctgcaag taaggctgag aaacctgctg
    54721 caaaagcaat ttgttttgta ttattatttt tactctttaa tgcaacagcc atcgaagcag
    54781 ccccttgagc taagtttgac cctaacattg ctggaagaat taatacgtct ggagtagcaa
    54841 tagatgccgc caaaaaaata ggtgcaaaag cccaatgcat tccagtcata acaataaatg
    54901 gcataatagc accaagaata gctaatgtaa gccatccagc tacaccatac atttgcccaa
    54961 ctagatttga taatccttca ccaacaatta ctccaatagg tccgactaca actaaggcaa
    55021 tacagcttga tactaataat actagcgtag gttgcaaaaa actcttagta acagctggtg
    55081 ttaatttagc aatcattttt tcaatatatt tcatcaacca aaccataata agaattggaa
    55141 cgactgacga accataacta gctggtgtca caggtacacc aaataaacta agaggattcc
    55201 ctgattgcac catttgaaca aaatttggat ggagaagtac acctgctaca gacatagcta
    55261 atgtagatgt tacttttaat ttttgtgatg cagaataagc taataacagc ggtaagaaat
    55321 aatatggcgc atccccaaaa aatgtcaaaa aagcaatagt ctgagaatct gattgcaata
    55381 taccaagcat tggtaaaata attaccaaga ctttcagcat acctccccct aacattgctg
    55441 gaatgattgg agtcatggaa ccagcgatat actcaatgat tctttctaaa atattccctt
    55501 tgtgcccttg aacaactgaa tcggattcaa aattgccaag tttaacgaat tctttataat
    55561 aattagctac atcattacca agtataattt gatattgtcc attctttttc ataataccta
    55621 ttacacctgg tatcttcttc atatcatcat cattgactaa attttcatct tttaattcta
    55681 atcttaaacg tgttacacaa tgggtaactc tattgacatt tttttcacct ccaattacat
    55741 cgaggatttt ttgtacctta tctttataac tcatggtatt ctcctattct attaatctaa
    55801 attttttgtt aagcgacgaa tatgagccat caaataaact aattcactag aagtcagcaa
    55861 ataattgtac tcagtttgta taaacattgc cacctgttca ccacattcat attctctagg
    55921 atatttattt ttcattaatg ctaacaagtc ttcatcatca tcgtcacaat aag
//
LOCUS       DAYYQN010000006        45171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYYQN010000006 DAYYQN010000000
VERSION     DAYYQN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.006"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            32..679
                     /locus_tag="ACHBRQ_000334"
                     /pseudo
     CDS             32..679
                     /locus_tag="ACHBRQ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(668..1472)
                     /locus_tag="ACHBRQ_000335"
     CDS             complement(join(668..1102,1104..1472))
                     /locus_tag="ACHBRQ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFM9592069.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTIMIDVTEVKINRPKKELANYSGKKKCYAMKAQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKFKPLTVEDKTYNHA
                     LSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            1449..1721
                     /locus_tag="ACHBRQ_000336"
     CDS             1449..1721
                     /locus_tag="ACHBRQ_000336"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9592070.1"
                     /translation="MFTCFIIHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSG
                     KIKHHEDSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            2016..3350
                     /gene="trmFO"
                     /locus_tag="ACHBRQ_000337"
     CDS             2016..3350
                     /gene="trmFO"
                     /locus_tag="ACHBRQ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HFM9592071.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            3434..4171
                     /gene="pyrH"
                     /locus_tag="ACHBRQ_000338"
     CDS             3434..4171
                     /gene="pyrH"
                     /locus_tag="ACHBRQ_000338"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9592072.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            4180..4737
                     /gene="frr"
                     /locus_tag="ACHBRQ_000339"
     CDS             4180..4737
                     /gene="frr"
                     /locus_tag="ACHBRQ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9592073.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAIKHIDDMTANKEKELLEV"
     gene            4797..5651
                     /gene="cvfB"
                     /locus_tag="ACHBRQ_000340"
     CDS             4797..5651
                     /gene="cvfB"
                     /locus_tag="ACHBRQ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HFM9592074.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5660..5875
                     /locus_tag="ACHBRQ_000341"
     CDS             5660..5875
                     /locus_tag="ACHBRQ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9592075.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5961..6929
                     /locus_tag="ACHBRQ_000342"
     CDS             5961..6929
                     /locus_tag="ACHBRQ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9592076.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            7122..7623
                     /locus_tag="ACHBRQ_000343"
                     /pseudo
     CDS             7122..7623
                     /locus_tag="ACHBRQ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            7626..7951
                     /locus_tag="ACHBRQ_000344"
                     /pseudo
     CDS             7626..7951
                     /locus_tag="ACHBRQ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032802.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(8252..9363)
                     /gene="ald"
                     /locus_tag="ACHBRQ_000345"
                     /pseudo
     CDS             complement(8252..9363)
                     /gene="ald"
                     /locus_tag="ACHBRQ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            9540..10109
                     /locus_tag="ACHBRQ_000346"
     CDS             9540..10109
                     /locus_tag="ACHBRQ_000346"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592077.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            10177..10827
                     /locus_tag="ACHBRQ_000347"
     CDS             10177..10827
                     /locus_tag="ACHBRQ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFM9592078.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10811..13051
                     /locus_tag="ACHBRQ_000348"
     CDS             10811..13051
                     /locus_tag="ACHBRQ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9592079.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            13230..13418
                     /locus_tag="ACHBRQ_000349"
     CDS             13230..13418
                     /locus_tag="ACHBRQ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592080.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            13451..14038
                     /locus_tag="ACHBRQ_000350"
     CDS             13451..14038
                     /locus_tag="ACHBRQ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9592081.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            14042..15226
                     /locus_tag="ACHBRQ_000351"
     CDS             14042..15226
                     /locus_tag="ACHBRQ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592082.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASPGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     misc_feature    15390..15521
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            15537..16067
                     /gene="infC"
                     /locus_tag="ACHBRQ_000352"
     CDS             15537..16067
                     /gene="infC"
                     /locus_tag="ACHBRQ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9592083.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            16100..16300
                     /gene="rpmI"
                     /locus_tag="ACHBRQ_000353"
     CDS             16100..16300
                     /gene="rpmI"
                     /locus_tag="ACHBRQ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9592084.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            16352..16711
                     /gene="rplT"
                     /locus_tag="ACHBRQ_000354"
     CDS             16352..16711
                     /gene="rplT"
                     /locus_tag="ACHBRQ_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9592085.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16769..17149
                     /locus_tag="ACHBRQ_000355"
     CDS             16769..17149
                     /locus_tag="ACHBRQ_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9592086.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            17399..18199
                     /locus_tag="ACHBRQ_000356"
     CDS             17399..18199
                     /locus_tag="ACHBRQ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9592087.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            18162..19148
                     /locus_tag="ACHBRQ_000357"
     CDS             18162..19148
                     /locus_tag="ACHBRQ_000357"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9592088.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            19190..21223
                     /locus_tag="ACHBRQ_000358"
     CDS             19190..21223
                     /locus_tag="ACHBRQ_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9592089.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(21284..22939)
                     /gene="pavA"
                     /locus_tag="ACHBRQ_000359"
     CDS             complement(21284..22939)
                     /gene="pavA"
                     /locus_tag="ACHBRQ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HFM9592090.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            23044..23541
                     /gene="ybeY"
                     /locus_tag="ACHBRQ_000360"
     CDS             23044..23541
                     /gene="ybeY"
                     /locus_tag="ACHBRQ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9592091.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            23522..23917
                     /locus_tag="ACHBRQ_000361"
     CDS             23522..23917
                     /locus_tag="ACHBRQ_000361"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9592092.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23934..24833
                     /gene="era"
                     /locus_tag="ACHBRQ_000362"
     CDS             23934..24833
                     /gene="era"
                     /locus_tag="ACHBRQ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9592093.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24881..25705
                     /gene="mutM"
                     /locus_tag="ACHBRQ_000363"
     CDS             24881..25705
                     /gene="mutM"
                     /locus_tag="ACHBRQ_000363"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9592094.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            25705..26310
                     /gene="coaE"
                     /locus_tag="ACHBRQ_000364"
     CDS             25705..26310
                     /gene="coaE"
                     /locus_tag="ACHBRQ_000364"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9592095.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEAIVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            26297..27496
                     /locus_tag="ACHBRQ_000365"
     CDS             26297..27496
                     /locus_tag="ACHBRQ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9592096.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            27496..27645
                     /gene="rpmG"
                     /locus_tag="ACHBRQ_000366"
     CDS             27496..27645
                     /gene="rpmG"
                     /locus_tag="ACHBRQ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9592097.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            27685..27918
                     /gene="secG"
                     /locus_tag="ACHBRQ_000367"
     CDS             27685..27918
                     /gene="secG"
                     /locus_tag="ACHBRQ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9592098.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            28020..30374
                     /gene="rnr"
                     /locus_tag="ACHBRQ_000368"
     CDS             28020..30374
                     /gene="rnr"
                     /locus_tag="ACHBRQ_000368"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9592099.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            30337..30804
                     /gene="smpB"
                     /locus_tag="ACHBRQ_000369"
     CDS             30337..30804
                     /gene="smpB"
                     /locus_tag="ACHBRQ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9592100.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            30820..31680
                     /gene="tehB"
                     /locus_tag="ACHBRQ_000370"
     CDS             30820..31680
                     /gene="tehB"
                     /locus_tag="ACHBRQ_000370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFM9592101.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            31753..32706
                     /gene="coiA"
                     /locus_tag="ACHBRQ_000371"
     CDS             31753..32706
                     /gene="coiA"
                     /locus_tag="ACHBRQ_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HFM9592102.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKGKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            32725..34527
                     /gene="pepF"
                     /locus_tag="ACHBRQ_000372"
     CDS             32725..34527
                     /gene="pepF"
                     /locus_tag="ACHBRQ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9592103.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            34529..35242
                     /locus_tag="ACHBRQ_000373"
     CDS             34529..35242
                     /locus_tag="ACHBRQ_000373"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9592104.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            35309..36250
                     /gene="prsA"
                     /locus_tag="ACHBRQ_000374"
     CDS             35309..36250
                     /gene="prsA"
                     /locus_tag="ACHBRQ_000374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HFM9592105.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(36369..36710)
                     /locus_tag="ACHBRQ_000375"
                     /pseudo
     CDS             complement(36369..36710)
                     /locus_tag="ACHBRQ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            37122..37217
                     /locus_tag="ACHBRQ_000376"
                     /pseudo
     CDS             37122..37217
                     /locus_tag="ACHBRQ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(37470..38090)
                     /locus_tag="ACHBRQ_000377"
     CDS             complement(37470..38090)
                     /locus_tag="ACHBRQ_000377"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9592106.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(38090..38572)
                     /locus_tag="ACHBRQ_000378"
     CDS             complement(38090..38572)
                     /locus_tag="ACHBRQ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9592107.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(38572..39414)
                     /locus_tag="ACHBRQ_000379"
     CDS             complement(38572..39414)
                     /locus_tag="ACHBRQ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9592108.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(39509..40309)
                     /locus_tag="ACHBRQ_000380"
     CDS             complement(39509..40309)
                     /locus_tag="ACHBRQ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFM9592109.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     TYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            40453..41832
                     /gene="glmU"
                     /locus_tag="ACHBRQ_000381"
     CDS             40453..41832
                     /gene="glmU"
                     /locus_tag="ACHBRQ_000381"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9592110.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAEQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            41842..42387
                     /locus_tag="ACHBRQ_000382"
     CDS             41842..42387
                     /locus_tag="ACHBRQ_000382"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9592111.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            42399..42713
                     /gene="macP"
                     /locus_tag="ACHBRQ_000383"
     CDS             42399..42713
                     /gene="macP"
                     /locus_tag="ACHBRQ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9592112.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            42730..43422
                     /locus_tag="ACHBRQ_000384"
     CDS             42730..43422
                     /locus_tag="ACHBRQ_000384"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9592113.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            43499..43990
                     /gene="rocS"
                     /locus_tag="ACHBRQ_000385"
     CDS             43499..43990
                     /gene="rocS"
                     /locus_tag="ACHBRQ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HFM9592114.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            44088..44678
                     /locus_tag="ACHBRQ_000386"
     CDS             44088..44678
                     /locus_tag="ACHBRQ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9592115.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            44687..>45171
                     /locus_tag="ACHBRQ_000387"
     CDS             44687..>45171
                     /locus_tag="ACHBRQ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9592116.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     E"
BASE COUNT        13718 a         8273 c        10020 g        13160 t
ORIGIN      
        1 tgcaaatttt taagaaggga ggtgatcttg catggacttt gaatattttt ataacagaga
       61 agcgaaaaga tttaacttct taaaagtacc ggagatatta gttgatagag aagaatttcg
      121 gggcttatca gcagaagcaa ttatccttta ttccatactt cttaaataga caggaatgtc
      181 atttaagaat aactggatag acaaggaagg cagagtattt atctatttta ctgtcgaaga
      241 aattatgaaa agaagaaata tctcaaagcc aactgccata aaaacattag atgagcttga
      301 tataaaaaaa ggaataggac tgatcgaaag agtaaggctt ggacttggta agccgaacat
      361 catttatgtt aaagacttta tgagtatatt tcaggtaaaa gaaaatgact tacagaagtc
      421 aaaaaactta acttcagaag taaaagattt taacctcaga agtaaagaaa atgaacttca
      481 agaggttaag aaccttgact ctaactatat agagaataat aagagtaagt atagtaagag
      541 agaatatagt tttggtgaaa acggacttgg aacatttcaa aatgtgtttt tagctgctga
      601 agatatatag gatttacaaa tcataatgaa ctcacagctt gagaattaca ttagacttcc
      661 tgcaaaacta gaatcctagt tcatgattga taataccagc aatcaaattc attcgtaatc
      721 cgaatcgttt acgatgattt cgataggttg ttgaaaacat tttaaacgtt tctactttgg
      781 caaagatgtt ctcaaccttg cttctctcct tagatagcgc atggttatag gttttatctt
      841 caactgttag cggcttgaat ttgctggatt tacgtggagt ttgtgcttga ggatatatct
      901 tcatgagccc ttgataacca ctgtcagcca agattttacc agcttgtccg atatttctgc
      961 gactcatttt gaacaacttc atatcatgac aatagttcac agtgatatcc aaagaaacaa
     1021 ttctcccttg acttgtgaca atcgcttgag ccttcatagc gtagcatttc tttttaccag
     1081 aataattcgc taattctttt tttagggcga ttgattttta cttccgtcac atcaatcatt
     1141 atcgtgtcct cagaactgag aggagttctt gaaatcgtaa caccactttg aacaagagtt
     1201 acttcaaccc attggctccg acggattaag ttgctttcgt gaataccaaa atcagccgca
     1261 atttgctcat aagttcgata ttctcgcaca tattgaagag tggccataag aaggtcttct
     1321 aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat
     1381 acagctaata tctcttcaaa agtcgtgcgc tgaacaccaa caagacgctt aaatcgtgca
     1441 tcagttagtt gtttacttgc ttcatcattc atagaactac tataccatat tttgtttcgc
     1501 aggaagtcta ttggaaagta agaaatattg aagctgaggc gattagaaga aattgtgagc
     1561 gtggtgctat tttttcaggt aaaataaaac atcacgaaga ttctcagttt aaaggagacc
     1621 actatgttga atgttatgct gttttagata atacggttat agcaagagat agaataacag
     1681 tccctatcga tccgttatgt ggaaaagatt ttatagagta gcatataatt gattcttaac
     1741 tggaatactc tctatctctt tacatcaaga aaatgactaa acagggaagt ttgccttctt
     1801 cccttttttt gttatactag tagaagaaaa aaatagaaag atttgtggga gtgaaaaagt
     1861 ccgggggact ttttcagctt gagccaagaa attcgagagc gaggaaagtc tgggggactt
     1921 tttcagcctg agccaagaaa ctcgaaatct cgtaagtctg attggctttt tcaatgtgaa
     1981 tcttaacttc acactcccaa agaggtatta gtgtcgtgtc tcaatcttat atcaatgtta
     2041 tcggtgctgg tttggcaggt tctgaagcag cttaccaaat cgcagagcgt ggtattccag
     2101 ttaaacttta tgaaatgcgt ggtgtcaagt ctacacccca gcataaaaca gacaattttg
     2161 ctgagttggt ttgttccaat tctttgcgtg gggatgcttt gacaaatgca gttggtcttc
     2221 tcaaggaaga aatgcgtcgc ttgggttctg ttatcttgga atctgctgag gctacacgtg
     2281 ttcctgccgg tggagccctt gcggtggatc gtgatggttt ctctcaaatg gtgaccgaaa
     2341 aagtggtcaa ccaccccttg attgaagtgg tgcgtgatga aattacagaa ttgccaacag
     2401 atgttattac agttgtggcg actggtccct tgactagcga tgccctggct gaaaagattc
     2461 atgcccttaa taatggcgat ggcttctatt tctacgatgc agcagcgcct attatcgatg
     2521 tcaacactat cgatatgagt aaggtctatc tcaaatctcg ttatgataag ggagaagcag
     2581 cctatctcaa tgctccaatg accaagcaag agtttatgga tttccatgaa gccttggtca
     2641 atgcggaaga agcaccgctc aattcttttg aaaaagaaaa gtactttgaa ggatgtatgc
     2701 caatcgaagt tatggccaaa cgtggcatta aaactatgct ttatggccct atgaagccag
     2761 tcggtcttga gtatccggac gactacacag gacctcgtga tggagaattt aaaacacctt
     2821 atgcggttgt gcaacttcgt caggataatg cagctggtag cctatacaat attgttggtt
     2881 tccagaccca cctcaaatgg ggagaacaaa agcgtgtctt ccaaatgatt ccgggtcttg
     2941 aaaatgcgga gtttgtccgt tatggtgtca tgcatcgcaa ttcttacatg gattcaccaa
     3001 atcttcttga gcagacttac cgttccaaga aacaaccaaa tctcttcttt gctggtcaga
     3061 tgacgggggt ggaaggctat gttgagtcgg cggcttcggg cttagttgcg ggaattaacg
     3121 cagctcgtct cttcaaggaa gaaagcgagg ttattttccc cgagacgaca gcgattggaa
     3181 gcttagctca ttacattacc catgccgaca gcaaacattt ccaaccaatg aatgtcaatt
     3241 ttgggatcat caaggagttg gaaggcgagc gtatccgtga taagaaggct cgttatgaaa
     3301 aaattgcaga gcgtgccctt gccgacttag aggaattttt gactgtctaa tttttttgaa
     3361 agaattgttc atgatactat aaaaatctta gaaattgtga taaaataggt aggatgaaag
     3421 aaggagagtg aaaatggcga atcccaagta taaacgtatt ttaatcaagt tatcaggtga
     3481 agcccttgcc ggtgaacgtg gcgtagggat tgatatccaa acagttcaaa caatcgcaaa
     3541 agagattcaa gaagttcata gcttaggtat cgaaattgcc cttgttattg gtggaggaaa
     3601 tctctggcgt ggagaacctg cagcagaagc aggtatggac cgtgttcagg cagattacac
     3661 tggaatgctt gggactgtta tgaatgctct tgtgatggca gattcattgc aacaagttgg
     3721 ggttgatacg cgtgtacaaa cagctattgc tatgcaacaa gtggcagagc cttatgtccg
     3781 tggacgtgcc cttcgtcacc ttgaaaaagg ccgtatcgtt atctttggtg ctggaattgg
     3841 ttcaccatac ttctcgacag atacaacagc ggcccttcgt gcagctgaaa tcgaagcaga
     3901 tgccatcctc atggctaaaa atggcgtcga tggtgtgtac aatgccgatc ctaagaagga
     3961 caagacagcc gttaagtttg aagaattgac ccaccgtgat gttatcaaca aaggtcttcg
     4021 tatcatggac tcaacagcct caaccctctc aatggacaac gacattgact tggttgtctt
     4081 caacatgaac caatcaggca acatcaaacg tgttgtattt ggtgaaaata tcggaacaac
     4141 agtttcaaat aatatcgaag aaaaggaata agaaagaata tggctaacgt aattattgaa
     4201 aaagctaaag agagaatgac ccagtctcac caatcacttg ctcgtgaatt tggtggtatc
     4261 cgtgctggtc gtgccaatgc aagcttgctt gaccgtgtac atgtagaata ctatggagtc
     4321 gaaactcctc ttaaccaaat cgcttcaatt acgattccag aagcgcgtgt tttgttggta
     4381 acaccatttg acaagtcttc attgaaagac atcgaacgtg ccttgaacgc ttctgatctt
     4441 ggtatcacac cggctaatga cggttctgtg attcgcttgg ttatcccagc tcttacagaa
     4501 gaaactcgtc gtgaccttgc taaagaagtg aagaaggtcg gcgaaaatgc taaagtggct
     4561 gtccgcaata tccgtcgcga tgctatggac gaagctaaga agcaagaaaa agcaaaagaa
     4621 atcactgaag acgaattgaa gactcttgaa aaagatattc aaaaagtaac agacgatgct
     4681 attaaacaca tcgacgacat gactgctaac aaagagaaag aacttttgga agtctaaaaa
     4741 taaacagaaa aactcagttg gcattgctgg ctgagtttta ttcgaaagaa ggaaatatga
     4801 atacaaatct tgcaagtttt atcgttggac tgatcatcga tgaaaatgac cgtttttact
     4861 ttgtgcaaaa ggatggtcaa acctatgctc ttgctaagga agagggccaa catacagtag
     4921 gggatacggt caaaggtttt gcatacacgg atatgaagca aaaactccgc ctgacaacct
     4981 tagaagtgac tgccactcag gaccaatttg gttggggacg tgtcacagag gttcgtaagg
     5041 acttgggtgt ctttgtggat acaggccttc ctgacaagga aatcgttgtg tcactcgata
     5101 ttctccctgt gctcaaggaa ctctggccta agaagggcga ccaactctac atccgtcttg
     5161 aagtggataa gaaagaccgt atctggggcc tcttggctta tcaagaagac ttccaacgtc
     5221 tcgctcgtcc tgcctacaac aacatgcaga accaaaactg gccagccatt gtttaccgtc
     5281 tcaagctgtc aggaactttt gtttacctac cagaaaataa tatgcttggt tttattcatc
     5341 ctagcgagcg ttacgcagag ccacgtttgg ggcaagtatt agatgcgcgc gttattggtt
     5401 tccgtgaagt ggaccgcact ctgaacctct ccctcaaacc acgctccttt gaaatgttgg
     5461 aaaacgatgc tcagatgatt ttgacttatt tggaaagcaa tggcggtttc atgaccttaa
     5521 atgacaagtc atctccagac gacatcaagg caacctttgg catttctaaa ggtcagttca
     5581 agaaagcttt aggtggtctt atgaaggctg gtaaaatcaa gcaagaccag tttgggacag
     5641 agttgattta gggaggctta tgagaaaatc attttacact tggctcatga ccgagcgcaa
     5701 tcctaaaagt aacagtccca aagcaatttt ggcagacctc gcttttgaag agtcagcctt
     5761 tccaaaacat acagatgatt ttgatgaggt cagtcgcttt ttggaggagc atgccagttt
     5821 ctcttttaac ctaggagatt ttgacagcat ttggcaggaa tatctagaac actagcattt
     5881 attcattggg tttgggctag taatttctcc atccctctgc tataataaaa agaaataaaa
     5941 ggattagaga ggttctttat ttgaaggaac attcaataga cattcaactg agtcatccag
     6001 atgacctgtt tcatcttttt ggttccaatg aacgccatct tcgtttgatg gaagaagagc
     6061 ttgatgttgt gattcatgct cgtacggaga ttgtccaggt tttgggagaa gagtctgcct
     6121 gtgaggaagc ccgtcaagtt attcaggctt tgatggtctt ggtaaatcgt gggatgaccg
     6181 ttggtacgcc agatgtagtc actgcgatta gcatggtcaa aaatgatgaa attgacaagt
     6241 ttgtcgccct ttacgaagaa gaaatcatca aagacaatac tgggaagcct atccgagtca
     6301 aaaccttggg tcaaaaactt tatgtggaca gtgtcaaaca gcatgatgtg acctttggaa
     6361 tcggacctgc agggacaggg aagaccttcc ttgcagtgac cttggcagtg actgccctta
     6421 aacgtgggca agtcaaacga attatcctga cacgcccagc ggtggaagca ggagagagtc
     6481 taggttttct tccgggtgat cttaaggaga aggtggatcc ttaccttcgt cctgtttacg
     6541 atgccttgta tcaaattctt gggaaagacc aaacgactcg tctcatggag cgtgaaatta
     6601 tcgaaattgc gccccttgcc tatatgcgtg gccggacctt ggatgatgcc tttgtcattc
     6661 tcgatgaggc gcaaaacacg accatcatgc agatgaagat gttcttgacg cgtttaggct
     6721 ttcattctaa gatgattgtc aatggagata tcagtcagat tgacctgcca cgtaatgtca
     6781 agtccggttt gattgatgct caagagaaac tcaagaacat ccatcagatt gactttgttc
     6841 atttttcagc caaggatgtg gttcgccatc cagttgtcgc tcagattatc cgagcctatg
     6901 aatattctac tgaagttgca cacgactgat tttgaggaag ttcgcctgca aaagaatata
     6961 cttttgagag aggaaaatcc agttgtatag gctaaaggtt ttatccaaag gtttgagaca
     7021 acgattaggc acgatggaaa gaacttttat gtggctgatg acgatcagtg catcttcctg
     7081 tgtcataatc acagggcaca agaaagtagg aatttgaaaa gatgattgac caactatcta
     7141 agtattacag ttgtaggata ctaactgaaa aggatattcc aagtatttta tctttatatg
     7201 aaagtaatcc tctgtatttt cagcattgtc caccagagcc aaattttgca actgtaaaag
     7261 aggacatgct ttgtctacct gaaggtaaag ctaaggctga taagtttttt tgttggattt
     7321 tggaatggat ctgaccttgt ggctgttatg gattttgtct atgcatatcc tgatgaggag
     7381 actgttttta ttggtttgtt tatggttgat ccagcctatc agaggaaagg gattggtagt
     7441 catattgtga cagaagcact agcttatttt gctaagaact ttcgaaaggc acgtttggct
     7501 tatgttaagg gaaatccgca atctcagcat ttttgggaaa agcagggctt taaatcaatt
     7561 ggatgcgagg ttaagaaaga actctatacg gttgttatcg ttgaacagag cctagaagat
     7621 tagaaatggc atcaagtaag aactatttgg aatttgtttt ggaacaatta tcaggattag
     7681 atgatgtgac ttaccgttcc atgatggggg agtatattct ttacttccgc ggcaagatta
     7741 ttggcggcat ttatgacgat cgcttttagt taaacccgtg caagcagtct tagataagat
     7801 tgaccaatct tcttttgagt ttccatacaa aggtgccaaa gaaatgattt gagtggaaga
     7861 acttgataat aagatgtttc tataagacct gattttagct atgtataacc aactgccaac
     7921 gcccaaacct aaaaagaaaa agcaagggtg aacgaagtaa aaaagaagtc tgctaaggcc
     7981 ctgtctttgc acgggtaaaa ttttatatat aaaaagaagc tgggactaaa gagctcagct
     8041 tcctttggtt tatataattg tcattacaag acgaagtggt tgggcgaaac tctgttgact
     8101 ttattcaatt tagagtttct tatgcacaat tgagtctgga acgaaagtct ccagttgcaa
     8161 agtatacagt acaataaacc aacgatgtaa tagctgatga cacaaagcac agtgggtagg
     8221 acttgcgaag tcaccctttt cttttcaaaa tttatactaa atcattgata tcagtgtagt
     8281 cacgattaag tccttgagca actggtaggc tagtcaagta accttgataa gtggtcacac
     8341 cttgacgcaa gccttcatct tcagagattg cttgtgcgaa tcctttgcca gccaaagctt
     8401 cgatataagg aagagtgaca ttggttaggg cgatggttga agtgcgggca accgcaccag
     8461 ggatattggc aacggcatag tggagaacac cgtgtttttc atagacgggt tcatcgtgcg
     8521 ttgtcacacg gtcagctgtt tcgataaggc caccttggtc aacagcaatg tcaacgatac
     8581 agagcctgga cgcatttgtt tgaccatctc atctgtcacc aattccggtg cttttgcacc
     8641 agggatgaga atggctccaa tcaccacatc agcatctctc acacttgctt caatgttgaa
     8701 tgaattagac ataagagttt gaatttgact tccaaagact tcttctagaa ctgagagacg
     8761 cttggcacta atatctaaaa tagtcacttg agcaccaaga ccaagggcga tgcgggcagc
     8821 atgtgtaccg acgacaccat caccgatgat agttactttt ccttttggaa cacctggtac
     8881 accaccaagt agaacaccag agccaccagc ttgcttagta aggaagtgag ctccgattta
     8941 aacagccata cgacctgcaa cctcactcat aggaacgagg agcggtagtt gtccttgatt
     9001 gtcacgaaca gtttcatagg caattcctgt tgtttttgct gctaacatag catctgctaa
     9061 ttctggagca gcggccatgt gcaagtaggt gaagagaaga agatcgtcgc gcaagtaacc
     9121 gtattcagaa cttaaaggtt cttttacttt cacaaccaac tctgctgccc aagcttcacc
     9181 agcagtagcg acaatctcag ctccttgctt ttgatagtca gcatcagtaa agccagaacc
     9241 gagaccagca tttgtttcga taaggacacg atgaccacga ctaactaagc tatgaacacc
     9301 tgcaggtgtg agggcgacac ggttttcgtt atttttaatt tcttttggga ttccgattaa
     9361 cattgagata acctaccttt caattgacgg tcttgttttg gttgtcacat tccagttcat
     9421 aaatcaaaaa tgtgacggtt tcattgtata tgaaaccgct tcaaaaatca agaaaaactt
     9481 gtcatccaaa ttttttatgc tagactagtg aaaatcaagc tttgattggt tggaaaagta
     9541 tggaatcaat ttttgtaaaa cttgcccagt atccgtctat agaaacggag cgtttattgc
     9601 ttagacctgt aactttggat gatgcggaag caatgtttga ctatgcctcg gacaagggta
     9661 atacacgtta cacttttcca accaatcaaa gcttggaaga aaccaagaat aacattgctc
     9721 agttctattt ggctaatccc ttgggacgtt ggggaataga actaaaaagc aatggtcagt
     9781 ttattggaac cattgacttg cacaagattg attctgttct taagaaggca gctattggct
     9841 acattatcaa taaaaagtat tggaatcaag gattaacgac agaagccaat cgtgctgtga
     9901 ttgagctagc ttttgagaag atagggatga ataagttgac tgcccttcac gataaggaca
     9961 atcctgcgtc aggaaaggtc atggagaaat caggcatgcg tttttcccat gcagaaccat
    10021 atgcttgtat ggaccagcat gaaaaagatc gaatcgttac aagagttcat tatgtcttga
    10081 ccaaggaaga ctattttgca aataaataag cagttgaaaa gaaatttttc gactgttttt
    10141 tcttcatctt acgaataatc taagagagga gaaaatatgg aagcaattat cgagaaaatc
    10201 aaagagtata aaatcatcgt catctgtact ggtctgggct tgcttgtagg cggatttttc
    10261 ctgctaaagc cagctccaca aacacctgtc aaagaaacga atttgcaggc tgaagttgca
    10321 gctgtttcca aggatttggt atccgaaaag gaagtgaaga aggaagagaa ggaagaaccc
    10381 cttgaacaag atctaatcac agtagatgtc aaaggtgctg tcaaatcgcc agggatttat
    10441 gacttgcctg taggtagtcg agtcaatgat gctgttcaga aggctggtgg cttgacagag
    10501 caagcagaca gcaagtcgct caatctagct cagaaagtta gtgatgaggc tctggtttac
    10561 gttcctacta agggagaaga agcagttagc caacagactg gtttggggac agcttcttca
    10621 ataagcaagg aaaagaaggt caatctcaac aaggccagtc tggaagaact caagcaggtc
    10681 aagggactgg gaggaaaacg agctcaggac attatcgacc atcgtgaggc aaatggcaag
    10741 ttcaagtcag tagacgaact caagaaggtc tctggcattg gtggcaaaac aatagaaaag
    10801 cttaaagact atgttacagt ggattaagaa tttctctatt cccctaattt acctgagttt
    10861 tctattactt tggctttatt acgctatttt ctcagcatct tatcttgctt tgttgggctt
    10921 tgtttttctg ctagtctgtc tctttatcca atttccgtgg aaatctgctg gtaaagttct
    10981 agtgatttgt ggaatctttg gattttggtt tgtttttcaa aattggcaac agagtcaagc
    11041 gagtcaaaat ctggcggatt ctgttgaagg ggtacggatt ttgcctgata ctattaaggt
    11101 taatggtgat agtctatcct ttcgtggcaa gtctaacggt cgtgctttcc aagtctatta
    11161 taaactccag tccgaggagg agaaagaagc ctttcaagct ttaaccgacc tgcatgagat
    11221 aggactagaa gggaagcttt cggagccaga agggcagaga aattttggtg gctttaatta
    11281 ccaagcctat ctgaagactc agggaattta ccagactctc aatattaaaa caatccagtc
    11341 acttcaaaag attggcagtt gggatatagg agaaaacttg tccagtttac gtcgaaaggc
    11401 tgtggtttgg attaagacgc actttccaga ccctatgcgc aattacatga caggactctt
    11461 gctgggacat ctggacaccg actttgagga gatgaatgag ctttattcca gtctaggaat
    11521 tatccacctc tttgccctat ctggtatgca ggtaggtttt ttcatgaatg gatttaagaa
    11581 acttctcttg cgattgggct tgacccaaga aaagttgaaa tggctgactt atcccttttc
    11641 ccttatctat gcgggactaa ctggattttc agcatcggtt attcgcagtc tcttgcaaaa
    11701 gctactggct caacatgggg ttaagggctt ggataatttt gccttgacgg tgcttgtcct
    11761 ctttattgtc atgccaaact ttttcttgac agcaggagga gtcttgtcct gcgcttatgc
    11821 ttttatcctg accatgacca gcaaagaagg ggaggggctc aaggctgtta ctagtgaaag
    11881 tctagtcatc tccttgggca tattgcccat tctatccttc tattttgcgg aatttcaacc
    11941 ttggtctatc cttttgacct ttgtcttttc ctttctattt gacttgacct tcttaccgct
    12001 cttgtctatt ttatttgtcc tttcctttct ctatccagtc attcagctga actttatttt
    12061 tgaatggttg gagggcatta ttcgcttggt gtcacaggtg acaagtagac ctctggtctt
    12121 tggacaaccc aatgcatggt ttttaatcct attgttaatt tccttggctt tggtctatga
    12181 tttgagaaaa aacattaaaa agctaacggt attgtgctta ttgattacag ggctctttct
    12241 cctgaccaag catccactgg aaaatgaaat caccatgctg gatgtggggc aaggagaaag
    12301 tattttccta cgggatgtaa ctgggaaaac cattctcata gatgtaggtg gtaaggcaga
    12361 atcttataag aaaatcgaaa aatggcaaga aaagatgacg accagcaatg cccagcgaac
    12421 cttgattccc tatctcaaaa gtcgaggagt agctaagatt gaccagctaa ttttgactaa
    12481 cacggacaag gagaatgttg gagatttgtc agaggtgacc aaggctttcc atgtagggga
    12541 gattctagta tcaaaagaca gtctgaaaca gaaggaattt gtggcagaac tacaggcgac
    12601 tcaaacaaag gtgcgtagta tgacagtagg ggagaacttg cccatttttg gaagtcagtt
    12661 agaagttcta tctccaagga aaatgggaga tggaggacac gatgataccc tagttctgta
    12721 tgggaaattc ttggataagc aatttctctt cacgggaaat ttggaggaga aaggagagaa
    12781 ggacttgctg aagcactatc cagacttgaa agtaaatgtt ttgaaagcta gccaacatgg
    12841 caataaaaaa tcatcaagtc cagcctttct agaaaaactc aaaccagagc ttactcttat
    12901 ctcagttgga aagagcaatc gaatgaaact cccccatcag gaaacattga cacgactgga
    12961 aggtatcaat agcaaagttt atcgaactga ccagcaagga gctatacgtt ttaaggggtt
    13021 ggatagttgg aaaatcgaaa gtgttcgata ggaaggataa atgttgtaga ttagtgaaat
    13081 aaactaaaaa tttgttgcat aataatgata aaaatggtat aatgaaaacg tattcaatat
    13141 tgaggatata aaatcattaa aaatcagcaa aagttgtttt attagttagt ttataatcta
    13201 ttggtcttct tcagtccagt gtatctgctg tgacagtcac taaaagttac aagtatgatt
    13261 ggaatacggt ttgggaatat agtaccaact atcacgacca tcagtatgct tggattccgt
    13321 catggtctcg ttatgacagc tattctgagt ataaagttgg cggaggctgg aactacgctc
    13381 gttatgaggt cataaactat tacagcggag gctattaatt cttaaagagt gagaaaaagg
    13441 agggctagat atgttgcagc ttactcatgt gaccttaaaa acgcgacaag tcatcttgca
    13501 agatgtggat ttcaccttta aaaagggtag ggtttatggt cttcttgcta tcaatggctc
    13561 tggaaagacg accctgttcc gtgccattag caatttaatt cccataagta gtggaaatat
    13621 cgcagcccct ccttctttat tttattatga gagtattgaa tggctggatg gaaacttaag
    13681 tgggatggac taccttcgtc ttatcaaaaa catctggaag tcaggtctga acttgaggga
    13741 tgaaatcgcc tattgggaaa tgtctgacta tatcagtctt cccattcgca agtattcctt
    13801 aggcatgaag caacgcttgg tgattgccat gtatttcctc agtcaggcca aatgctggct
    13861 catggatgag attacaaatg gcttagatga gtattatcga cagaagtttt ttgataggct
    13921 agcacaaatc gatagacaag aacagctggt tcttttaagt tcccactata aggaagagtt
    13981 ggttgatgtc tgcgatagag tagtaaccat tcatcagggg cagatagaag aggtttagtt
    14041 tatgaaagat gttagtctat ttttattgaa aaaagttttc aaaagccgct taaactggat
    14101 tgtcttagct ttatttgtat ctgtactcgg tgttaccttt tatttaaata gtcagactgc
    14161 aaactcacac agcttggaga gcaggttgga aagtcgcatt gcagccaacg agagggctat
    14221 caatgaaaat gaagagaaac tctcccaaat gtctgatacc agctcggagg aataccagtt
    14281 tgctaaaaat aatttagacg tgcaaaaaaa tcttttgacg cgaaagacag aaattctaac
    14341 tttactaaaa gaagggcgct ggaaagaagc ctactatttg cagtggcaag atgaagagaa
    14401 gaattatgaa tttgtatcaa atgacccgac tgctagccct ggcttaaaaa tgggggttga
    14461 ccgcgaacgg aagatttacc aagccctgta tcccttgaac ataaaagcac atactttgga
    14521 gtttccgacc cacgggattg atcagattgt ctggatttta gaggttatca tcccaagttt
    14581 gtttgtggtt gctattattt ttatgctaac acaactattt gcagaaagat atcaaaatca
    14641 tctggacaca gctcacttat atcctgtttc aaaagtgaca tttgcaatat cctctcttgg
    14701 agttggagtg ggatatgtaa ctgtgctgtt tatcggaatc tgtggctttt cttttctagt
    14761 gggaagtctg ataagtggtt ttggacagtt agattatccc tacccaattt atagcttagt
    14821 gaatcaagaa gtaactattg ggaaaataca agatgtatta tttcctggct tgctcttagc
    14881 tttcttagcc tttatcgtca ttgtggaagt tgtgtacttg attgcttact ttttcaagca
    14941 aaaaatgcct gtcctctttc tttcactcat tgggattgtt ggcttattgt ttggtatcca
    15001 aaccattcag cctcttcaaa ggattgcaca tctgattccc tttacttact tgcgttcagt
    15061 ggagatttta tctggaagat tacctaagca gattgataat gtcgatctaa attggagcat
    15121 gggaatggtc ttacttcctt gcctgattat ctttttgcta ttgggaattc tatttattga
    15181 aagatgggga agttcacaga aaaaagaatt ttttaataga ttctagcttt cctataggta
    15241 gggaaaataa gtaaaaacta acatagagag ggaatcaact tgattctctc tttttgattc
    15301 gaaaaccaaa ccaaaataca aacacaaact tttcaaaaaa taacttttta tcttgacaag
    15361 agctagaaaa cttggtatca tataaaagtt gagaaaagca gaagtgagag cttctcgcct
    15421 tgtgacatta agttgcctgg ccctacggat gaaaagtttc gaagaaacgc tatcataacg
    15481 tgcgggcttg tatatttaca agtccgctat tgtttttctc taataaaaca aaagaggtga
    15541 aaaccatagc aaagcaagac ttattcatca atgatgagat tcgtgtacgt gaagttcgct
    15601 tgattggtct tgaaggagaa cagctaggta tcaagccact cagtgaagcg caagctttgg
    15661 ctgataacgc taatgttgac ctagtattga ttcaacccca agccaaaccg cctgttgcaa
    15721 aaattatgga ctacggtaag ttcaaatttg agtaccagaa gaagcaaaaa gaacaacgta
    15781 aaaaacaaag cgttgttact gtgaaagaag ttcgtctaag tccaactatt gacaagggtg
    15841 actttgatac aaaacttcgt aatgcacgta agttccttga aaaaggaaat aaagttaagg
    15901 tatctattcg ctttaagggt cgtatgatta cccataaaga gattggtgca aaagttttag
    15961 ccgagtttgc tgaagcaact caagatattg ccatcatcga acaacgtgct aaaatggatg
    16021 gacgtcaaat gttcatgcaa ttggcgccag cgactgacaa aaaataattg tcagaaagtt
    16081 aaataaagga gaaaaaatca tgccaaaaca aaaaacacac cgcgcatcag ctaaacgttt
    16141 caaacgtaca ggttctggtg gacttaaacg tttccgtgct tacacttctc accgtttcca
    16201 cggaaaaact aagaaacaac gtcgtcatct tcgtaaagca tctatggtgc attcaggaga
    16261 ttacaaacgt atcaaagcaa tgcttactcg cttgaaataa tagcttgact gtaagtaaac
    16321 aattctagga aatatttgga ggaaataaaa catggcacgt gttaaaggtg gcgttgtatc
    16381 acgcaaacgt cgtaaacgta ttcttaaatt agcaaaaggt tactatggag ctaaacacat
    16441 cttgttccgt actgcaaaag aacaagtaat gaactcttac tactatgcat accgtgaccg
    16501 tcgtcagaaa aaacgtgact tccgcaaatt gtggattact cgtatcaatg cggcagctcg
    16561 tatgaacgga ctttcatact cacaattgat gcatggtttg aaattggctg agattgaagt
    16621 taaccgtaaa atgcttgctg acttggctgt taacgatgca gtagctttca cagctcttgc
    16681 agatgcagct aaagcaaaac ttggtaaata ataactgatg agactggaac aggattgtcc
    16741 agtcttttta aaaataaagg agaaagatat ggcttcaaaa atgctacaca cttgcttacg
    16801 agtagaaaat cttgaaaaat caatcgcatt ttatcaagat gcttttggtt ttaaagaatt
    16861 gcgtcgcaga gattttccag accatgcctt cacgattgtc tatctaggtc ttgagggtga
    16921 tgattatgag ttggagttga cttataacta cgatcacggt ccgtatgtgg ttggtgatgg
    16981 gtttgcccat atcgccctca gtacacctga tcttgaggca cttcatcaag aacacagtgc
    17041 aaaaggctat gaagtgactg agccaaatgg tctaccagga actacaccaa actattactt
    17101 tgttaaagac cctgatggat ataaggtcga agtcattcgt gaaaaataat ttcgtgcaag
    17161 ttaagtagaa tattcgtttt ctacttgact ttttttagct gaaagagtat actaataaaa
    17221 attcaacctt taaggggagt ccagagagac tcacaaggtg tcagataaaa gaatagtgca
    17281 attttctaga ggagaccttt tgagtgtgct ctcttgtgtt gtacgatttt aactgaggcc
    17341 ttgcactagc aaggtctttt ctttatctgg tccccttaaa atttaaggag gaaaagttat
    17401 gaatctcaca tgtaagaagc gtttgggtgt cattcggttg gaaaccatga aggtggttgc
    17461 acaagaggaa atcgcgccag caatctttga attagtccta gaaggagaaa tggttgaagc
    17521 catgcgagca ggccaatttc ttcatctgcg tgtaccagac gatgcccatc tcttacgtcg
    17581 tcctatttca atttcgtcta ttgacaaggc aaacaagcag tgtcacctca tttatcggat
    17641 tgagggagct gggactgcaa ttttttcaac cttaagtcag ggagacactc ttgatgtgat
    17701 ggggcctcag ggaaatggtt ttgacttgtc tgaccttgat gagcagaatc aggttctcct
    17761 tgttggtggt gggattggtg ttccaccctt gcttgaggtg gccaaggaat tgcatgaacg
    17821 tggagtgaaa gtagtgacag tcctcggttt tgctaataag gatgctgtta ttttgaaaac
    17881 ggaattggct cagtatggtc aggtctttgt aacgacagat gatggttctt atggcatcaa
    17941 gggaaatgtt tccgttgtta tcaatgattt agatagtcag tttgatgctg tttactcgtg
    18001 tggggctcca ggaatgatga agtatatcaa tcaaaccttt gatgatcacc caagagccta
    18061 tttatctctg gaatctcgta tggcttgtgg gatgggagct tgctatgcct gtgttctaaa
    18121 agtaccagaa agcgagacgg tcagccaacg cgtctgtgaa gatggtcctg ttttccgcac
    18181 aggaacagtt gtattataag gagaaaatta tgactacaaa tcgattacaa gtgtctctac
    18241 ctggtttgga tttgaaaaat ccgattattc cagcatcagg ctgttttggc tttggacaag
    18301 agtatgccaa gtactatgat ttaaaccttt taggttctat tatgatcaag gcgacaaccc
    18361 ttgaaccacg ttttgggaac ccaactccaa gagtagcaga gacgcctgct ggtatgctca
    18421 atgcaattgg cttgcaaaat cctggtttag aggttgtttt ggctgaaaag ctaccttggc
    18481 tggaaagaga atatccaaat cttcctatta ttgccaatgt agctggtttt tcaaaacaag
    18541 agtatgcagc tgtttctcat gggatttcca aggcaactaa tgtaaaagct atcgagctca
    18601 atatttcttg tcccaatgtt gaccactgta atcatggact tttgattggt caagatccag
    18661 atttggctta tgatgtggtg aaagcagctg tggaagcctc agaagtgcca gtttatgtca
    18721 aattaacccc gagtgtgacc gatatcgtta ctgtcgcaaa agctgcagaa gatgcgggag
    18781 caagtggctt gaccatgatc aatactctgg ttggaatgcg ctttgacctc aaaaccagaa
    18841 aaccaatctt ggccaatgga acaggtggaa tgtcaggtcc agcagtcttt ccagtagccc
    18901 tcaaactcat ccgccaagta gcccaaacaa cagacctgcc tatcattgga atggggggag
    18961 tggattcggc tgaagctgcc ctagaaatgt atctggctgg ggcatctgct atcggagttg
    19021 gaacagctaa ctttaccaat ccttatgcct gccctgacat catcgaaaat ttaccaaaag
    19081 tcatggataa atacggtatt agcagtctgg aagaactccg tcaggaagta aaagagtctc
    19141 tgaggtaaac tgcaatcaat ctgttcttga ttttttatta gtttgtaata tgaatttagg
    19201 agaattttgg tacaataaaa taaataagaa cagaggaaga aggttaatga agaaagtaag
    19261 atttattttt ttagctctgc tatttttctt agctagtcca gagggtgcaa tggctagtga
    19321 tggtacttgg caaggaaaac agtatctgaa agaagatggc agtcaagcag caaatgagtg
    19381 ggtttttgat actcattatc aatcttggtt ctatataaaa gcagatgcta actatgctga
    19441 aaatgaatgg ctaaagcaag gtgacgacta tttttacctc aaatctggtg gctatatggc
    19501 caaatcagaa tgggtagaag acaagggagc cttttattat cttgaccaag atggaaagat
    19561 gaaaagaaat gcttgggtag gaacttccta tgttggtgca acaggtgcca aagtaataga
    19621 agactgggtc tatgattctc aatacgatgc ttggttttat atcaaagcag atggacagca
    19681 cgcagagaaa gaatggctcc aaattaaagg gaaggactat tatttcaaat ccggtggtta
    19741 tctactgaca agtcagtgga ttaatcaagc ttatgtgaat gctagtggtg ccaaagtaca
    19801 gcaaggttgg ctttttgaca aacaatacca atcttggttt tacatcaaag aaaatggaaa
    19861 ctatgctgat aaagaatgga ttttcgagaa tggtcactat tattatctaa aatccggtgg
    19921 ctacatggca gccaatgaat ggatttggga taaggaatct tggttttatc tcaaatttga
    19981 tgggaaaatg gctgaaaaag aatgggtcta cgattctcat agtcaagctt ggtactactt
    20041 caaatccggt ggttacatga cagccaatga atggatttgg gataaggaat cttggtttta
    20101 tctcaaatct gatgggaaaa tagctgaaaa agaatgggtc tacgattctc atagtcaagc
    20161 ttggtactac ttcaaatccg gtggttacat gacagccaat gaatggattt gggataagga
    20221 atcttggttt tacctcaaat ctgatgggaa aatagctgaa aaagaatggg tctacgattc
    20281 tcatagtcaa gcttggtact acttcaaatc tggtggctac atggcgaaaa atgagacagt
    20341 agatggttat cagcttggaa gcgatggtaa atggcttgga ggaaaaacta caaatgaaaa
    20401 tgctgcttac tatcaagtag tgcctgttac agccaatgtt tatgattcag atggtgaaaa
    20461 gctttcctat atatcgcaag gtagtgtcgt atggctagat aaggatagaa aaagtgatga
    20521 caagcgcttg gctattacta tttctggttt gtcaggctat atgaaaacag aagatttaca
    20581 agcgctagat gctagtaagg actttatccc ttattatgag agtgatggcc accgttttta
    20641 tcactatgtg gctcagaatg ctagtatccc agtagcttct catctttctg atatggaagt
    20701 aggcaagaaa tattattcgg cagatggcct gcattttgat ggttttaagc ttgagaatcc
    20761 cttccttttc aaagatttaa cagaggctac aaactacagt gctgaagaat tggataaggt
    20821 atttagtttg ctaaacatta ataatagcct tttggagaac aagggcgcta cttttaagga
    20881 agccgaagaa cattaccata tcaatgccct ttatctcctt gcccatagtg ccctagaaag
    20941 taactgggga agaagtaaaa ttgccaaaga taagaataat ttctttggca ttacagccta
    21001 tgatacgacc ccttaccttt ctgccaagac atttgatgat gtggataagg gaattttagg
    21061 tgcaaccaag tggattaagg aaaattatat cgatagggga agaactttcc ttggaaacaa
    21121 ggcttctggt atgaatgtgg aatatgcttc agacccttat tggggcgaaa aaattgctag
    21181 tgtgatgatg aaaatcaatg agaagctagg tggcaaagat tagtactata agtgaatatg
    21241 atttgagtga atagtaagtt aaaaatcctg atttcaagta aaatcaggat tttttcatgg
    21301 atgcaatttt tttggagtct ggtgtgacgc ggagggtctt ttgtcctgtg taagtgacaa
    21361 agccgggttt tccaccagtt ggtttattga gttttttgac ttcaatcata tctacctgca
    21421 ccagattcga caggcgccct tgagagaagt aggcagctaa ctctgctgcg tctgtcttga
    21481 ctgcatcaga tgggtcaaga tttcctgaga tgacaacatg gcttccagga atgtccttag
    21541 catggaacca aagttcctcc ttgcgggcca ttttaaaggt caattcctca ttttgaagat
    21601 tgtttcgtcc gacatagatg atggttttgc catcgcttgc tagatattgt tctagttttt
    21661 tgcgtttctg gattttctcc cgttgtcttc tgcggataaa acctgtttga atcaattctt
    21721 cacggatttc agcgatttct tccagtccag cttggttgag gacggtttct acactttcca
    21781 gatagagaat agtggctttg gtttcttcaa tcaaatcagt caagtatttg acagcttctt
    21841 tgagtttctg ataccgttta aaatagcgtt gggcattctg gttgggagtc agagccttat
    21901 caagcgcaat catgataggt tggttggtat agtagttgtc taggataacc tggtcttggt
    21961 cgttaggcac ttggtggagg aaggttgtca gcaattctcc tttttgacga aattcttcag
    22021 cgttgtctgt cgccagtaac tctttttcct gttttttgag tttgtgtcgg tttttctgaa
    22081 gttcattttc aacacgacga atcagttcac tggcctgctg tttgacgcgg tcgcgctcag
    22141 ccttatcctt atagtaggtg tccaacaaat cagaaagatt tgcaaaaggc tctcccacct
    22201 gatttgcaaa aggaactgga ctgaaggaag tctcagtcaa gcatggcttg gtttcctgat
    22261 tgaaaaaatt tcggaaagcg gaaagttttt cactaaccag tatcctttcc aattcatttg
    22321 ccgtatcgcg tcccagacct tgaaagaggc tttgaagatt ttttgctgtt agttcttggg
    22381 tttgcaggat ttcaaagagc ttttcatcct tgatagtaaa aggattgaga gattctgtac
    22441 ttggcggagc gatataggtc gatcctggaa gtaaggtgcg gtagctattt tgtgaaaaac
    22501 cgacgtgttt gataacttcg aggattttat gactgctttt atcgaccagt agaatattac
    22561 tgtgtttccc cataatttcg ataatcaagg tagcctggat atggtctcca atctcgtttt
    22621 tattggaaac tgtcatttcc acaatacggt cattttccac ttgctcaatc gactcaatca
    22681 gggccccctg caaatacttt ctcaaaacca tgataaaggt agaaggttgg gctggatttt
    22741 caaaagtcgt ttgggtcagc tgaatgcgtc caaaaactgg atgggcagaa aggagcaggc
    22801 gatggctttg gcgattgctg cggatttgca agaccaactc ttgttcaaaa ggctgattga
    22861 ttttctggat gcgaccattc actaattcgc ttcgcaattc ctcaactatg tggtgtaaaa
    22921 aaaatccgtc aaatgacatc gttctctcct tgtgattgta ttccatagta ttatatcaaa
    22981 aaggtagaat aaaatcatgg aaatgtggta taataaagcc aagtaaagag aaacgagaag
    23041 cacatgtata ttgaaatggt agatgaaact ggtcaagttt caaaagaaat gttgcaacaa
    23101 acccaagaaa ttttggaatt tgcagcccaa aaattaggaa aagaagacaa ggagatggca
    23161 gtcacttttg tgaccaatga gcgtagtcat gaacttaatc tggagtaccg tgacaccgac
    23221 cgtccgacag atgtcatcag ccttgagtat aaaccagaat tggaaattgc ctttgacgaa
    23281 gaggatttgc ttgaaaatcc agaattggca gagatgatgt ctgagtttga tgcctatatt
    23341 ggggaattgt tcatctctat cgataaggct catgagcagg ccgaagaata tggtcacagc
    23401 tttgagcgtg agatgggctt cttggcagta cacggctttt tacatattaa cggctatgat
    23461 cactacactc cggaagaaga agcggagatg ttcggtttac aagaagaaat tttgacagcc
    23521 tatggactca caagacaata aacgaaaatg gaaaaatcgt gacctgatat ccagtttaga
    23581 atttgccatc acagggattt ttactgctat caaggaagaa cgaaatatgc gaaaacatgc
    23641 agtgacggct ctagtggtta tccttgcagg ttttgttttt caggtgtcac gaatcgaatg
    23701 gctctttctc ctattgagca ttttcttggt agtagccttt gagattatca actctgctat
    23761 tgaaaatatg gtggatttgg ccagtcacta tcacttttcc atgctggcta aaaatgccaa
    23821 ggatatggcg gctggcgcgg tattagtggt ttctcttttc gcagccttaa caggtgcatt
    23881 gatttttctc ccacgaatct gggatttatt attttaaaca gtaagaggaa attatgactt
    23941 ttaaatcagg ctttgtagcc attttaggac gtcccaatgt tgggaagtca acctttttaa
    24001 atcacgttat ggggcaaaag attgccatca tgagtgacaa ggcgcagaca acgcgcaata
    24061 aaatcatggg aatttacacg actgataagg agcaaattgt ctttatcgac acaccaggga
    24121 ttcacaaacc taaaacagct ctcggagatt tcatggttga gtctgcctac agtacccttc
    24181 gcgaagtgga caccgttctt ttcatggtgc ctgctgatga agcgcgtggt aagggggacg
    24241 atatgattat cgagcgtctc aaggctgcca aggttcctgt gattttggtg gtgaataaaa
    24301 tcgataaggt ccatccagac cagctcttgt ctcagattga tgacttccgt aatcaaatgg
    24361 actttaagga aattgttcca atctcagccc ttcagggaaa taacgtgtct cgtctagtgg
    24421 atattttgag tgaaaatctg gatgaaggtt tccaatattt cccgtctgat caaatcacag
    24481 accatccaga acgtttcttg gtttcagaaa tggttcgcga gaaagtcttg catctaactc
    24541 gtgaagagat tccgcattct gtagcagtag ttgttgactc tatgaaacga gacgaagaga
    24601 cagacaaggt tcacatccgt gcaaccatca tggttgagcg cgatagccaa aaagggatta
    24661 tcatcggtaa aggtggcgct atgcttaaga aaatcggtag catggcccgt cgtgatatcg
    24721 aactcatgct aggagacaag gtcttcctag aaacctgggt caaggtcaag aaaaactggc
    24781 gcgataaaaa gctagatttg gctgactttg gctataatga aagagaatac taagtagagg
    24841 taggctcatg cctgcttctt gtttttacag aaggagactt atgcctgaat tacctgaggt
    24901 tgaaaccgtt tgtcgtggct tagaaaaatt gattatagga aagaagattt cgagtataga
    24961 aattcgctac cccaagatga ttaagacgga tttggaagag tttcaaaggg aattgcctag
    25021 tcagattatc gagtcaatgg gacgtcgtgg aaaatatttg cttttttatc tgacagacaa
    25081 ggtcttgatt tcccatttgc ggatggaggg caagtatttt tactatccag accaaggacc
    25141 tgaacgcaag catgcccatg ttttctttca ttttgaagat ggtggcacgc ttgtttatga
    25201 ggatgttcgc aagtttggaa ccatggaact cttggtgcct gaccttttag acgcctactt
    25261 tatttctaaa aaattaggtc ctgaaccaag cgaacaagat tttgatttac aggtctttca
    25321 atctgccctt gccaagtcca aaaagcctat caaatcccat ctcctagacc agaccttggt
    25381 agctggactt ggcaatatct atgtggatga ggttctctgg cgagctcagg ttcatccagc
    25441 tagaccttcc cagactttga cagcagaaga agcgactgcc attcatgacc agaccattgc
    25501 tgttttgggc caggctgttg aaaaaggtgg ctccaccatt cggacttata ccaatgcctt
    25561 tggggaagat ggaagcatgc aggactttca tcaggtctat gataagactg gtcaagaatg
    25621 tgtacgctgt ggtaccatca ttgagaaaat tcaactaggc ggacgtggaa cccacttttg
    25681 tccaaactgt caaaggaggg actgatggga aaaatcatcg gaatcactgg gggaattgcc
    25741 tcaggtaagt caactgtgac aaattttcta agacagcaag gctttcaagc agtggatgcc
    25801 gacgcagtcg ttcaccaact acagaaacct ggtggtcgtc tgtttgaggc tatagtacag
    25861 cactttgggc aagaaatcat tcttgaaaac ggagaactca atcgccctct cctagctagt
    25921 ctcatctttt caaatcctga agagcaaaaa tggtctaatc aaattcaagg ggagattatc
    25981 cgtgaggaac tggctacttt gagagaacaa ttggctcaga cagaagagat tttcttcatg
    26041 gatattcccc tactttttga gcaggactac agcgattggt ttgctgagac ttggttggtc
    26101 tatgtggacc gagatgccca agtagaacgc ttaatgaaaa gggaccagtt gtccaaagat
    26161 gaagctgagt ctcgtctggc agcccagtgg cctttagaaa aaaagaaaga tttggccagc
    26221 caggttcttg ataataatgg caatcagaac cagcttctta atcaagtgca tatccttctt
    26281 gagggaggta ggcaagatga cagagattaa ctggaaggat aatctgcgta ttgcctggtt
    26341 tggtaatttt ctgacaggag ccagtatttc tttggttgta ccttttatgc ccatcttcgt
    26401 ggaaaatcta ggtgtaggga gtcagcaagt cgctttttat gcaggcttag caatttctgt
    26461 ctctgctatt tccgcggcgc tcttttctcc tatttggggt attcttgctg acaaatacgg
    26521 ccgaaaaccc atgatgattc gggcaggtct tgctatgact atcactatgg gaggcttggc
    26581 ctttgtccca aatatctatt ggttaatctt tcttcgttta ctaaacggtg tatttgcagg
    26641 ttttgttcct aatgcaacgg cactgatagc cagtcaggtt ccaaaggaga aatcaggctc
    26701 tgccttaggt actttgtcta caggcgtagt tgcaggtact ctaactggtc cctttattgg
    26761 tggctttatc gcagaattat ttggcattcg tacagttttc ttactggttg gtagttttct
    26821 atttttagct gctattttga ctatttgctt tatcaaggaa gattttcaac cagtagccaa
    26881 ggaaaaggct attccaacaa aggaattgtt tacttcagtt aaatatccct atcttttgct
    26941 caatctcttt ttaaccagtt ttgtcatcca attttcagct caatcgatcg gccctatttt
    27001 ggctctttat gtacgcgact tagggcagac agagaatctt ctttttgtct ctggtttgat
    27061 tgtgtccagt atgggctttt ccagcatgat gagtgcagga gtcatgggca agctaggtga
    27121 caaggtgggc aatcatcgtc tcttggttgt cgcccagttt tattcagtca tcatctatct
    27181 cctctgtgcc aatgcctcta gcccccttca actaggactc tatcgtttcc tctttggatt
    27241 gggaaccggt gccttgattc ccggggttaa tgccctactc agcaaaatga ctcccaaagc
    27301 cggcatttcg aggatctttg ccttcaatca ggtattcttt tatctgggag gtgttgttgg
    27361 tcccatggca ggttctgcag tagcaggtca atttggctac catgctgtct tttatgcgac
    27421 aagcctttgt gttgccttta gttgtctctt taacctgatt caatttcgaa cattattaaa
    27481 agtaaaggaa atctagtgcg agtaaaaatt aatctcaaat gctcctcttg tggcagtatc
    27541 aattacctaa ccagtaaaaa ttcaaaaacc catccagaca agattgaggt tttaaagtat
    27601 tgtcccaagg aaagaaaagt aaccctacat cttgaatcta agtagatttt atggtaaaat
    27661 aatagggatt ttaaggagtt tgatatgtat aacctattat taaccatttt attagtatta
    27721 tctgttgtga ttgtgattgc aattttcatg caaccaacca aaaaccaatc cagcaatgta
    27781 tttgatgcca gttcaggtga tttgtttgaa cgcagtaaag ctcgcggttt tgaagctgta
    27841 atgcagcgtt tgacagggat tttagtcttt ttctggctag ccattgcctt agcattgacg
    27901 gtattatcaa gtagataaga aaataatggg caggactagg tctttgcctc tttttatttt
    27961 taaaggatgt ttgagaaggt tttacagtaa aagaaaatta aaaaatctag aaagaaaata
    28021 tgaaagatag aataaaagaa tatttacaag acaagggaaa ggtgactgtt aatgatttgg
    28081 ctcaggcttt gggaaaagac agttccaagg attttcgtga gttgattaaa accttgtcct
    28141 taatggaaag aaagcaccag attcgctttg aagaagatgg tagtctgaca ttagaaatta
    28201 agaaaaaaca tgagattacc ctcaagggga tttttcatgc ccataaaaat ggctttggct
    28261 ttgttagtct ggaaggcgag gaggacgacc tttttgtagg gaaaaatgat gtcaactatg
    28321 ctattgatgg tgataccgtc gaggtagtga ttaagaaagt cgctgaccgc aataagggaa
    28381 cagcagcaga agccaaaatt attgatatcc tagaacacag tttgacaaca gttgtcgggc
    28441 aaatcgttct ggatcaggaa aagcctaagt atgctggcta tattcgttca aaaaatcaga
    28501 aaatcagtca accgatttat gttaagaaac cagccctaaa gttagaagga acagaagttc
    28561 tcaaagtctt tatcgataaa tacccaagca agaaacatga tttctttgtc gcgagtgttc
    28621 tcgatgtagt ggggcactca acggatgtcg gaattgatgt tcttgaggtc ttggaatcaa
    28681 tggacattgt atctgagttt ccagaagctg ttgtcaagga agcagaaagt gtgcctgatg
    28741 ctccgtctca aaaggatatg gaaggtcgtc tggatctaag agatgaaatt acctttacca
    28801 ttgacggtgc ggatgccaag gacttggacg atgcagtgca tatcaaggct ctgaaaaatg
    28861 gcaatctgga gcttggggtt cacatcgcag atgtttccta ttatgtgacc gagggttctg
    28921 cccttgacaa ggaagccctt aaccgtgcga cttctgttta cgtgacagac cgagtggtgc
    28981 caatgcttcc agaacgacta tcaaatggca tctgctctct caatccccaa gttgaccgcc
    29041 tgacccagtc tgctattatg gagattgata aacatggtcg tgtggtcaac tataccatta
    29101 cacaaacagt tatcaagacc agttttcgta tgacctatag cgatgtcaat gatatcctag
    29161 ctggcgatga agaaaagaga aaagaatacc ataaaattgt accaagtatc gaactcatgg
    29221 ccaagcttca tgaaacttta gaaaacatgc gtgtgaaacg tggagctctc aattttgata
    29281 ccaatgaagc gaagatttta gtggataaac aaggtaagcc tgttgatatc gttcttcggc
    29341 agcgtggcat tgccgagcgg atgattgagt cttttatgtt gatggctaat gaaacagttg
    29401 ccgaacattt cagcaagttg gatttgcctt ttatctatcg aattcacgag gagcctaagg
    29461 ctgaaaaggt tcagaagttt attgattatg cttcgagttt tggcttgcgc atttatggga
    29521 ctgccagtga gattagccag gaggcgcttc aagacatcat gcgtgctgtt gagggagaac
    29581 cttatgcaga tgtattgtcc atgatgcttc ttcgctctat gcagcaggct cgttattcgg
    29641 agcacaatca cggccactat ggactagctg ctgactatta tactcacttt accagtccaa
    29701 ttcgtcgtta tccagacctt cttgttcacc gtatgattcg ggattacggc tgttctaagg
    29761 aaatagcaga gcattttgaa caagtgattc cagagattgc gacccagtct tccaaccgtg
    29821 aacgtcgtgc catagaagct gagcgtgaag ttgaagccat gaaaaaggct gagtatatgg
    29881 aagaatacgt gggtgaagag tatgatgcag ttgtatcaag tattgtcaaa ttcggtctct
    29941 ttgtcgaatt gccaaacaca gttgaaggct tgattcacat cactaatcta cctgaatttt
    30001 atcatttcaa tgagcgtgat ttgactcttc gtggagaaaa atcaggtatc actttccgag
    30061 tgggtcagca gatccgtatc cgtgttgaaa gagcggataa aatgactgga gagattgatt
    30121 tttcattcgt acctagtgag tttgatgtga ttgaaaaagg cttgaaacag tctagtcgta
    30181 gtggcagagg gcgtggttca aatcgtcgtt cggataagaa ggaagacaag agaaaatcag
    30241 gacgctcaaa tgataagcgt aagcattcac aaaaagacaa gaagaaaaaa ggaaagaaac
    30301 ctttttacaa ggaagtagct aagaaaggag ccaagcatgg caaagggcga gggaaaggtc
    30361 gtcgcacaaa ataaaaaggc acgccacgac tatacaatcg tagatacgct agaggcaggg
    30421 atggtcctga ctggaactga aatcaagagt gtacgagctg ctcgaattaa tctcaaggat
    30481 ggctttgctc aagtgaaaaa tggagaagtt tggctgagta atgttcatat cgcgccttac
    30541 gaagagggca atatctggaa ccaggaacca gaacgtcgtc gtaaactcct gctccataaa
    30601 aagcaaattc aaaaattgga acaagagacc aaagggacag gaatgacctt agttcccctt
    30661 aaggtctata taaaagatgg ctacgctaag cttcttttag gacttgccaa agggaagcat
    30721 gactatgaca aacgggagtc tatcaaacgt cgtgagcaaa atcgagatat cgcgcgtgtg
    30781 atgaaagctg ttaatcagcg ataaaaagag gaattgaaaa tggaaaaatt agttgcctat
    30841 aaacgcatgc ctttgtggaa taaacaaaca atgcctgaag ctgttcagca aaagcacaat
    30901 acaaaagttg ggacttgggg gaaaattact gtcttgaagg gagctctcaa gtttattgaa
    30961 ttgacagaag aaggggaagt tctagctgaa cacctctttg aagcaggggc agacaatcca
    31021 atggcccaac ctcaagcctg gcaccgagtg gaagctgcca cagatgatgt ggaatggtac
    31081 ttggaatttt attgtaaacc tgaggattat tttgctaaaa aatacaatac caatcctgtt
    31141 cattcagagg tcctagaggc catgcagaca gtgaaacaag ggaaagcttt ggatttgggt
    31201 tgtggtcagg ggcgtaattc tctttttcta gcccagcaag attttgatgt gacggctgta
    31261 gatcaaaatg gactagctct tgaaatcttg caaagcattg tggagcagga agatttggac
    31321 atgcctgttg gcctttacga tatcaattca gctagcattg aacaagaata tgattttatc
    31381 gtttcaacag ttgttctcat gtttctacaa gcggaccgca ttccagctat tattcaaaat
    31441 atgcaggaga aaaccagtgt tggtggttac aaccttatcg tttgtgccat ggacacggag
    31501 gattatcctt gctcggttaa cttcccattc acctttaaag aaggagaact ggcagactat
    31561 tacaaggatt gggaattggt taagtacaat gaaaatccag gccatttgca ccgtcgcgat
    31621 gagaatggca atcgtattca actacgcttt gcgaccttac tagctaagaa aatcaagtaa
    31681 acacacatga agattaggaa ttttcctgat cttttttctt ttttacgaat gatatagaaa
    31741 aggagggaat tcatgtttgt tgcgagagat gctaggggag aattggtaaa tgtgttagag
    31801 gataaacttg agaagcaagc atacacctgc ccagcttgtg gaggccagct ccatttgcgt
    31861 caaggaccaa gtgtacggac gcattttgcc cataaatctt taaaagactg tgattttttc
    31921 tttgaaaatg aaagtccaga acacctggcc aataaggaat ccctctatca ctggttgaaa
    31981 aaagagacaa aggttcaatt agagtacccg ctttcagaac ttaaacagat tgcggatgta
    32041 tttgtaaatg gcaatctagc tctagaagtt cagtgtagtc ccttgcctca gaaagtcctt
    32101 aaagagcgaa gtgagggcta tcgtagtcag ggttaccaag tactgtggtt gctgggtcaa
    32161 aaactgtggc tcaaggagcg tttgactcgt ctacagcaag gttttcttta tttcagtcaa
    32221 aacatgggct tttatgtttg ggaattagac aagggaaaac aagttttaag actcaaatac
    32281 ctgatttacc aggatctccg cggtaaactc cattatcaaa tcaaggaatt ttcctatggc
    32341 caaggtagtt tattggaaat attgcgtctt ccctataaga aacaaaaaat atctcatttt
    32401 acagtttctg aggacaagga catctgtcgc tatatccggc aacaacttta ttatcaaaat
    32461 ctcttttgga tgaaagaaca agcagaagcc tatcaaaagg gagaaaatat cctgacttat
    32521 ggactgaaag aatggtatcc acaaattcga ccaatagtgg gcaaattttt ccagattgaa
    32581 caagacttga ctagctatta tcagcacttt tatacctatt accaaaaaaa tcctcaaaat
    32641 gattggcaaa agctttatcc accagccttt tatcagcaat atttcttgaa aaatatggta
    32701 gaatagaaag gatggaggaa tctaatggta ttacaaagaa atgaaataaa tgaaaaagat
    32761 acatgggatc tatcaacgat ctacccaact gaccaggctt gggaagaagc cttaaaagat
    32821 ttaacagaac aattggagac agtagcccag tatgaaggcc atctcttgga tagtgcggat
    32881 aacctactag aaatcactga attttctctt gaaatggaac gccagataga gaagctttac
    32941 gtttatgctc atatgaagaa tgaccaggat acacgtgaag ctaagtatca agagtactat
    33001 gccaaggcca tgacactcta cagccagtta gaccaagcct tttcattcta tgagcctgaa
    33061 tttatggaga ttagcgaaaa gcagtatgct gactttttag aagctcaacc aaagctgcag
    33121 gtttatcaac actattttga caagctcttg caaggcaagg atcacgttct ttcacaacgt
    33181 gaagaagaat tattggctgg agctggagaa atctttggtt cagcaagtga aaccttcgct
    33241 atcttggaca atgcggatat tgtgttccct tatgtcctag acgatgatgg taaagaagtt
    33301 cagctatctc atgggactta cacacgtttg atggagtcta aaaaacgtga ggttcgccgt
    33361 ggtgcctatc aagctcttta tgcgacttac gaacaattcc aacacaccta tgccaaaacc
    33421 ttgcaaacca atgttaaggt gcaaaattac cgtgctaaag ttcgtaacta caagagtgct
    33481 cgtcatgcag ccctcgcagc gaattttgtt ccagaaagtg tttatgacaa tttggtagca
    33541 gcagttcgca agcatttgcc actcttacat cgctatcttg agcttcgttc aaaaatcttg
    33601 gggatttcag atctcaagat gtacgatgtc tacacaccgc tttcatctgt tgaatacaat
    33661 tttacctacc aagaagcctt gaaaaaagca gaagatgctt tggcagtctt gggtgaggat
    33721 tacttgagcc gtgtcaaacg tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa
    33781 ggcaagcgtt caggtgccta ctctggtggt tcttacgata ccaatgcctt tatgcttctc
    33841 aactggcagg acaatctgga caatctcttt actcttgttc atgaaacagg tcacagtatg
    33901 cattcaagct atactcgtga aactcagcct tatgtttacg gagattactc tatctttttg
    33961 gctgagattg cctcaactac caatgaaaat atcttgacgg agaaattatt ggaagaagtg
    34021 gaagacgacg caacacgctt tgctattctc aataacttcc tagatggttt ccgtggaaca
    34081 gttttccgcc aaactcaatt tgctgagttt gaacacgcca ttcaccaagc agatcaaaat
    34141 ggggaggtct tgacaagcga tttcctaaat aaactctacg cagacttgaa ccaagagtat
    34201 tatggtttga gtaaggaaga caatcctgaa atccaatacg agtgggctcg cattccacac
    34261 ttctactata actactatgt atatcaatat tcaactggct ttgcggccgc ctcagccttg
    34321 gctgaaaaaa ttgtccatgg tagtcaagaa gaccgtgacc gctatatcga ctacctcaag
    34381 gcaggtaagt cggactatcc acttaatgtc atgagaaaag ctggtgttga tatggagaag
    34441 gaagactacc tcaacgatgc ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc
    34501 cttgttgaaa aattaggatt ggcataaaat ggttgaatcg tatagtaaga atgctaacca
    34561 taacatgcgt cgtcctgtcg tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa
    34621 gcaggtcaca ggtttcttga aagaattgga agactttgcc cgcaaggaaa atattcctat
    34681 tattccccat gaaacggttg cttatttccg ttttcttatg gaaaccatgc agcctaaaaa
    34741 tattctggaa attgggacgg ctatcggttt ttcagctctc ttgatggctg aacatgcgcc
    34801 aaatgctaag attacaacta ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa
    34861 ttttgcccag tttgacagtc gcaagcaaat cactctccta gagggagatg cggttgatgt
    34921 cttatctaca ctgacagagt cttatgattt cgtctttatg gattctgcca agtctaaata
    34981 catcgtcttt ctgccagaaa tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga
    35041 tgatattttt caaggtggtg atgttgccaa ggatattatg gaagtccgtc gtggtcagcg
    35101 aaccatttat cgaggccttc aaaaattatt tgatgcaacc ttagacaatc cagaactcac
    35161 cgcaacatta gtgcctttag gagatggtat tctcatgctt cgtaaaaatg tagcagatgt
    35221 tcaactgtct gaaagcgaat gattttcaga aaaatttaag aaaaaatagt aaaatagata
    35281 gagtaacact tatctcaaag gagtagacat gaagaaaaaa ttattggcag gtgccatcac
    35341 actattatca gtagcaactt tagcagcttg ttcgaaaggg tcagaagggg cagaccttat
    35401 cagcatgaaa ggggatgtca tcacagaaca tcaattttat gagcaagtga aaaacaaccc
    35461 ttcagcccaa caagtcttgt taaatatgac catccaaaaa gtttttgaaa aacaatatgg
    35521 ctcagagctt gatgataaag aggttgatga tactattgcc gaagaaaaaa aacaatatgg
    35581 cgaaaactac caacgtgtct tgtcacaagc aggtatgact cttgaaacac gtaaagctca
    35641 aattcgtaca agtaaattag ttgagttggc agttaagaag gtagcagaag ctgaattgac
    35701 agatgaagcc tataagaaag cctttgatga gtacactcca gatgtaacgg ctcaaatcat
    35761 ccgtcttaat aatgaagata aggccaaaga agttctcgaa aaagccaagg cagaaggtgc
    35821 tgattttgct caattagcca aagataattc aactgatgaa aaaacaaaag aaaatggtgg
    35881 agaaattacc tttgattctg cttcaacaga agtacctgag caagtcaaaa aagccgcttt
    35941 cgctttagat gtggatggtg tttctgatgt gattacagca actggcacac aagcctacag
    36001 tagccaatat tacattgtaa aactcactaa gaaaacagaa aaatcatcta atattgatga
    36061 ctacaaagaa aaattaaaaa ctgttatctt gactcaaaaa caaaatgatt caacatttgt
    36121 tcaaagcatt atcggaaaag aattgcaagc agccaatatc aaggttaagg accaagcctt
    36181 ccaaaatatc tttacccaat atatcggtgg tggagattca agctcaagca gtagtacatc
    36241 aaacgaatag tccaaatcaa tgagtcaggg aaaaaactcg acttcaggaa aaaatgaagc
    36301 aaacattccc acaataaaac gcatagtaca aggtttgtac tgccccccaa aaaagttaga
    36361 caattaattt atccgaagga tttagttctg tattgcacag agctaagtcc ttttagtttt
    36421 atcttaattc tcttattgtt gtaataatca atatagtcta taatggcttg ttccaattga
    36481 ttaagtgatt taaatgtttg ctcatagcca taaaacattt cggattttaa aatgccaaag
    36541 aaagattcca tcctaccgtt gtcttggctg tttcctttgc gtgacataga tgcttgaatt
    36601 cccttactct ctaggaagcg atgataagaa tcgtgttggt attgccagcc ttggtcacta
    36661 tggagaatcg tattctcgta gtgcttctct ttgaatgcct gttccaacat taacgatcaa
    36721 tcaatttaat catgtaccta agattagaat tgtttatccc aaatttattt gaaagcttct
    36781 ctaagctata tccttgtttt ctaagttcat agatctgaac tttatcatca taagttaatt
    36841 tcataataaa aacaccccaa aagttagatt ttttctgtct aacttttggg gtgtagttca
    36901 tgtacacctg atatgatgcg ttttataatt ttaaagactt tttgaccagc ctcatttttt
    36961 aacttgatac tcagtgaaaa gcaaagatta aactaggaag ctagctgtag gctgctcaaa
    37021 gaacagcttt gaggttgcag ataaaacttg tgaggtaacc aacatatata atgtgaagct
    37081 gacgtggttt gaatagattt taggagggta tgagtctgga agttttaatg gataatgcaa
    37141 gattccatag aatgggtaag ctagagttct tatgtgaaga gtttgggcat aaacttttac
    37201 cttttcctcc ctactcatct tagtatagaa aagtgaatct gaaatagtac ataactgctt
    37261 ctaaaacatt cttataaatt gatttaaatt ctcaaatcat attattcagt tcttatttca
    37321 ttttgctcta caatcctgtt gagaagacac gtgttcatat caaaaaggta ttggcaagtt
    37381 gcaatacctt tttacaaggt tcttttgtct tatttttgtt tcaactgact atatctccta
    37441 tggttctagt tcagaaggct aggctataat tatgattgat aagaagtatc attccaagta
    37501 ttgagagtga atgtttcaaa atcatgggtt tctataatgg tcaggctggc atttgctaga
    37561 ccgccatctt tacgaagaag tggttcttta tagcctagga gagtacgaag actggcagta
    37621 agattggcgc cgtgtccgac aattagaata cgttcagctg gactatcttt taatgatttg
    37681 ataaattgga tggtccgttg agttgtacta tagagggatt cggctccgaa cattcgagtg
    37741 tcaaattgag caagatttga acgaaaagcc tggatttgtt gcgggtaaat agcttccaag
    37801 gttgcaattt tcaaaccttc taacttccca agttgccatt cacggagatt aggaacgatt
    37861 tctaaagaac agggggtata gagttgactt tggataatct cagcagattt gaccgctcga
    37921 ggtaaatcac ttgaataaat ctgatcaaaa ggaatttcct tgagatactg accaagtcgt
    37981 tttagggttt caatggattc aggaagaaga ggagaatcac cactagcacc ttgaaaacga
    38041 ccttcttggt tccagagggt acgaccgtgg cggacaaagt agagtttcat tacttgatgt
    38101 cctccaaaat atctacaaag tctgccttta caaagctagc caagtcttgt ggcgcgacga
    38161 taatgctgtg tccgacttcg cctgcagaga caatcatttg atccaaatct agagcaattt
    38221 tatcgataaa aatgggataa ttgtgtttct gacgaattcc gacaggatta ttggctccat
    38281 gaatgtaacc agttgttttt tctaagtcct tttgtggaat catgctcact tttttattgc
    38341 cagaaatttt agctagtttc ttttcagaca agtgctgagt gatagggaca attccgataa
    38401 tcggtccggt cttgtctccc aaaagcgcca aggttttgaa aatctgatct cgttcataac
    38461 cttgaggaag ctctccttct agggcattga tttgaatccc ctgatgaggg atagctgctt
    38521 tagataggat ttgttccacc aatgtttttt tgattttaac tttttttgcc attatttata
    38581 tttatcctcc aattgactca tccaaatacc aagccagatt cccagcgcaa agaagaaggc
    38641 gatgatgaca taaccgacaa gtgaaagtcc tgtgtattgg atactttcag cgtttcctgc
    38701 atttggaatt aagatcaaaa gggtacttga taggacgata ccgatgatga aatgatagac
    38761 acgtgagtgg tagttgttta aggcataatc catcaatttt gaaaaaacga tgagagttgc
    38821 acctgcacca attccaatcg gaaagaaggt tcccaagaaa tcaaaagttt taaaaccagt
    38881 caacatagga gcatagagtc ccaaaatcaa aagtaaattt gatgggctga ggccaggaac
    38941 caagacgcca agggccaata gtgcgcctgc taggacgaag ttaagaaagc tggcgcttaa
    39001 ggttccaacg acaaaattta aggcatagag tcctaatcca gaaatgataa aggttgtcca
    39061 taaccaagct aaatcaatct tgtctcggtc agattctcga gttgattctt tgaggaggct
    39121 aggaactgta ccgataatag ctcccgcaaa gctccataat acaaaaacct gataattttc
    39181 aagcaggtat tcaatcgggt aggaaaataa gccgattccc agaagcatac cgatggcaac
    39241 tggaataaag tacaaaacat tttctttaaa gtctttaaag ggatgggcca gaaagccaat
    39301 cattcgttca tagattccta agattgctgc tagaacccct ccggaaattc ccggtaggat
    39361 aaatccaaga gcaattacaa tccctttaat aacgcgtgct aaccatgaga gcatatattt
    39421 cctccaacta tttctatttc aaaaaatcct tactatattg tatcacaatc agacacaaaa
    39481 agaaaaagca aatgataaac aaatgctttt aaggttttaa aaaaagtttt cgaaaggttt
    39541 cttctttatt ttttaaggga gagataacgt tgatatctaa atcgtggtca aagccggcaa
    39601 tttttccttt agatgtgtat tggtgaatat cataatctaa atcagtttta ggactgctct
    39661 ccaaaaatcc tgagtctgag ccgtaggacg gaatccaaac agaggtaaac ttgcctgtat
    39721 caatactgtg ttcttccatg aagtagacac caacgtagat gccgatgttt ttagcaccta
    39781 gtgatgctag ttttgctcga aagttttcga caccttcgtt catattagac atggttttgt
    39841 cttccacgtc aagccaatag taactagggc tgtaaggaga ggcagcattg tagaaaactt
    39901 cggcagcctt ttccatttct tggacacttt ttccagctac ataagcgtag acagcaactg
    39961 ggacattccg cttttgaagt tcagtgatat gactcttata ggtcttgtct attccattga
    40021 taaatgaagc atcattttct tttgttgttt gagcaccact gtgaacacga acaatagcac
    40081 ctgaaatatt ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc cagccagaga
    40141 ggtcaataat cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt gctatattgg
    40201 attttgtttt aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa atgaacatca
    40261 taatcccaaa aaaactaaat aaaataagtg gatgaatttg ttttctcata tcttataatt
    40321 ctaccctaaa aatcaaaaaa aatcaaaaaa atgggttaag gaagagactt tagagcattt
    40381 tttcattcaa gagtgcggaa tgatttgaaa tatggtataa taaaagggaa tttctacaga
    40441 aaagagaaga ttatgtcaaa ttttgccatt attttagcag cgggtaaagg gactcgcatg
    40501 aaatctgatt tgccaaaagt tttgcacaag gttgcgggta tttctatgtt ggaacatgtt
    40561 ttccgtagtg tgggagctat ccaacctgaa aagacagtaa cagttgtagg acacaaggca
    40621 gaattggttg aggaggtctt ggctgaacag acagaatttg tgactcaatc tgaacagttg
    40681 ggaactggtc atgcagttat gatgacagag cctatcttag aaggtttgtc aggacacacc
    40741 ttggtcattg caggagatac tcctttaatc actggtgaaa gcttgaaaaa cttgattgat
    40801 ttccatatca atcataaaaa tgtggccact atcttgactg ctgaaacgga taatcctttt
    40861 ggctatggac gaattgttcg taatgacaat gctgaggttc ttcgtattgt tgagcagaag
    40921 gatgctacag attttgaaaa gcaaatcaag gaaatcaaca ctggaacata cgtctttgac
    40981 aacgagcgtt tgtttgaggc tttgaaaaat atcaatacca ataacgctca aggcgaatac
    41041 tatattacag acgtcattgg tattttccgt gaaactggtg aaaaagttgg cgcttatact
    41101 ttgaaagatt ttgatgaaag tcttggggta aatgaccgtg tggcgcttgc gacagctgag
    41161 tcagttatgc gtcgtcgcat caatcataaa cacatggtca acggtgttag ctttgtcaat
    41221 ccagaagcaa cttatatcga tattgatgtt gagattgctc cggaagttca aatcgaagcc
    41281 aatgttatct tgaaagggca aacgaaaatt ggtgctgaga ctgttttgac aaacggtact
    41341 tatgtagtgg acagcactat cggagcagga gcggtcatta ccaattctat gattgaggaa
    41401 agtagtgttg cagacggtgt gacagtcggt ccttatgctc acattcgtcc aaattcaagt
    41461 ctgggtgccc aagttcatat tggtaacttt gttgaggtga aaggatcttc aatcggtgag
    41521 aataccaagg ctggtcattt gacttatatc ggaaactgtg aagtgggaag caacgttaat
    41581 ttcggtgctg gaactattac agtcaactat gacggcaaaa acaaatacaa gacagtcatt
    41641 ggagacaatg tctttgttgg ttcaaattca accattattg caccagtaga acttggtgac
    41701 aattccctcg ttggtgctgg ttcaactatt actaaagacg tgccagcaga tgctattgct
    41761 attggtcgcg gtcgtcagat caataaagac gaatatgcaa cacgtcttcc tcatcatcct
    41821 aagaaccagt aggagcctat catggagttt gaagaaaaaa cgcttagccg aaaagaaatc
    41881 tatcaaggac caatatttaa actggtccaa gatcaggttg aattaccaga aggcaaggga
    41941 actgcccaac gggatttgat tttccacaat ggggctgtct gtgttttagc agtaacggat
    42001 gaacaaaaac ttatcttggt caagcagtac cgcaaagcta tcgaggcagt ctcttacgaa
    42061 attccagccg gaaaattgga ggtaggagaa aacacagccc ctgtcgcagc tgcccttcgt
    42121 gaattagagg aagaaacagc ctatacaggg aaattagaac tcttgtacga tttttattca
    42181 gctattggct tttgtaatga gaagttaaaa ctatatttag caagcgattt gacaaaagtg
    42241 gaaaatccgc gtccgcagga tgaggatgaa accttggaag tccttgaagt gagcttagaa
    42301 gaagcgaaag aattaatcca atcaggtcat atctgtgatg ccaagacaat tatggctgtt
    42361 cagtattggg agttgcagaa aaaatagagg aggtcagtat gggtaaatct ttattaacgg
    42421 atgaaatgat tgaaagagct aatagaggcg aaaaaatttc aggtcctcct ttgctagatg
    42481 ataatgagga gactaagatt ttaccaacct cttcttcccg ttttggttat gccaatccta
    42541 aggatcatgg ttttagccag gaaaccttga aaattcaggt cgaaccatct attcataaaa
    42601 gccgtcgcat tgaaaatacc aagagaaatg tcttcaattc taagttgaat aaaatcttat
    42661 tcgcggtcat ctttctcttg attttgcttg ttttagcaat gaaacttttg taatagaaaa
    42721 ggaattgaaa tgaaaatagg aattattgct gctatgccag aagaactggc ttatctggtc
    42781 cagcatttag ataataccca ggagcaagtt gttttgggga atacctatca tacaggaacc
    42841 attgcttctc atgaagtcgt tcttgtagaa agtggaattg gtaaggtcat gtctgctatg
    42901 agtgtggcga ttttggctga tcatttccag gtggatgccc ttattaatac gggttcagct
    42961 ggggcagtag cagaaggtat cgctgttggg gatgtcgtga ttgctgacaa attagcctat
    43021 catgacgtgg atgtcacagc ttttggctat gcttatggac aaatggcgca acaaccgctt
    43081 tatttcgaat cagacaaaac ctttgttgct caaatccaag agagtttatc tcaattggac
    43141 caaaactggc atcttggttt gattgctaca ggagatagtt ttgttgcagg aaatgacaag
    43201 atagaagcga ttaagtccca tttcccagaa gttttagccg tggagatgga gggggcagct
    43261 attgctcaag cagcgcatgc cctcaatctc ccagtcttag tcatcagagc tatgagtgac
    43321 aatgccaacc atgaagcaaa catctttttt gatgagttta ttatcgaagc tggacgtcgc
    43381 tctgcccaag tcttgttgac ctttttgaag gctttagatt aagcggaaat ttgacagttt
    43441 ttctagctta tgataagatt taagtaaaga aaagctagaa aacgtttcag aggatattat
    43501 gagtattgaa atgaccgtca gtgagattgc agaggtctta ggattatctc gccaagcaat
    43561 caataaccgt gtcaaagaat taccagaaga agacacagat aaaaatgaca aaggggtaac
    43621 agttgttacc agaagtggct tgattaagct agaagaaatc tataaaaaaa cgatttttga
    43681 agatgagcct gtcagtgaag atgtcaaaca acgtgaactg atggagattc tagtggatga
    43741 gaagaatgca gaaattcttc gtctttatga acaattaaaa gccaaggatc gtcagttatc
    43801 agaaaaagac gagcagatgc gtatcaaaga ccgtcagatt gctgaaaaag acaaacaatt
    43861 agatcagcaa caacaattga cccttcaagc catgaaggat caagaaaatc ttaagctaga
    43921 gctggaccaa gcaaaagaag aagtccaatc cactaagaaa ggcttttttg ctcgtttatt
    43981 tggaggataa tcaaggagct gtttaggtta aatgctaacc tgatggcttc ttttgtttgt
    44041 gaatagtata gttgattaga acagagacag tagaggagag gtacagaatg gaacgattag
    44101 aagattacat tgcttttgac ttagaattta atcagcacga aggacaaaca cacttgattc
    44161 aagtatcggc agtgcgtttt agagatggtc gggaaatcga tgcctatgat tcctatgttc
    44221 ataccagcgt ccctctaaaa agctttatca atggtttgac cgggattaca gctgagacct
    44281 taaaagatgc tcctccagtt gagaaagtct tagaagaatt ccaagagttt gtgggttctt
    44341 tgctgatgat aggctacaat gctgctaaaa gtgatttacc tattttagca gagcatggtt
    44401 tagactatag ccaacagtac aaggtggatt tgtatgatga agcttttgaa cgtcggagtt
    44461 cagatttaca tggtattgca aatctcaaat tgcaaactgt tgcctcattt cttggatttc
    44521 aaggtcagtc tcataatagt ttagaggatg ctcggatgac ggcgcgtgtt tacgaatcct
    44581 tcttagagtc ggatcaagct agggaattgt tagagacaga aagtagctta tcaaacaatc
    44641 cttttggtgg cttggacttg tctcaattat ttgactagga gtgcctatga aacctgaaaa
    44701 acctaaaaaa ttgggtctta aacagattta cctcaatctt gataaaatcc tctttctatt
    44761 tttcttgatt tttatgatag tggagtattt gtgggtacca ttcaattcat ggttggctag
    44821 cttcttatta gatcagacag gttatctttt tatttcctat aacaatgtct tagccatcct
    44881 tactcattct ccattgatta gtctagcttt tttagccttg attgtaatta accttctggt
    44941 tgcttatttt caaataggac tcctctttat tggtgctcgt catctcctct ataatgaaaa
    45001 gcgaaggcta attgagtata tccgcaaggt tttccatgaa agttttggat ttatgaaaaa
    45061 gctaacttgc gctaaagcct tgtttatctt tttctatata gctatgcttt ttccttttat
    45121 acggaaaatg ctgaagattt attatcttaa taaaatcgtc attccagagt t
//
LOCUS       DAYYQN010000007        44435 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYYQN010000007 DAYYQN010000000
VERSION     DAYYQN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.007"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            332..808
                     /locus_tag="ACHBRQ_000388"
     CDS             332..808
                     /locus_tag="ACHBRQ_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9592117.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            846..1151
                     /locus_tag="ACHBRQ_000389"
     CDS             846..1151
                     /locus_tag="ACHBRQ_000389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9592118.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            1199..2674
                     /locus_tag="ACHBRQ_000390"
     CDS             1199..2674
                     /locus_tag="ACHBRQ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFM9592119.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            3024..9725
                     /gene="bgaA"
                     /locus_tag="ACHBRQ_000391"
     CDS             3024..9725
                     /gene="bgaA"
                     /locus_tag="ACHBRQ_000391"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HFM9592120.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9973..10419)
                     /locus_tag="ACHBRQ_000392"
     CDS             complement(9973..10419)
                     /locus_tag="ACHBRQ_000392"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9592121.1"
                     /translation="MFDKEQKRGYKQSAKNLANWHYNDKEDSYIHPDGWCYRFHHIKY
                     QKTQTDFQQEIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQDRQIFAQRKI
                     DVEPVFGQIKACLGYKRCNLRGKRQVRIDMGLVLMANNLLKHSEMK"
     gene            complement(10584..10829)
                     /locus_tag="ACHBRQ_000393"
     CDS             complement(10584..10829)
                     /locus_tag="ACHBRQ_000393"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9592122.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTLEERHFYTS
                     YHAFGRPSYHPKMLVSTLLFAYSQGIFSGRKIEKWKS"
     gene            complement(11040..11399)
                     /locus_tag="ACHBRQ_000394"
     CDS             complement(11040..11399)
                     /locus_tag="ACHBRQ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9592123.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            11491..12447
                     /locus_tag="ACHBRQ_000395"
                     /pseudo
     CDS             11491..12447
                     /locus_tag="ACHBRQ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12431..12988
                     /locus_tag="ACHBRQ_000396"
     CDS             12431..12988
                     /locus_tag="ACHBRQ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9592124.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12981..13235
                     /locus_tag="ACHBRQ_000397"
     CDS             12981..13235
                     /locus_tag="ACHBRQ_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592125.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            13247..15301
                     /locus_tag="ACHBRQ_000398"
     CDS             13247..15301
                     /locus_tag="ACHBRQ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9592126.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15480..16340
                     /locus_tag="ACHBRQ_000399"
     CDS             15480..16340
                     /locus_tag="ACHBRQ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9592127.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            16551..17261
                     /gene="ccdA2"
                     /locus_tag="ACHBRQ_000400"
     CDS             16551..17261
                     /gene="ccdA2"
                     /locus_tag="ACHBRQ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HFM9592128.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            17281..17847
                     /locus_tag="ACHBRQ_000401"
     CDS             17281..17847
                     /locus_tag="ACHBRQ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFM9592129.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17858..18970
                     /gene="msrB"
                     /locus_tag="ACHBRQ_000402"
     CDS             17858..18970
                     /gene="msrB"
                     /locus_tag="ACHBRQ_000402"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9592130.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            19283..20020
                     /locus_tag="ACHBRQ_000403"
     CDS             19283..20020
                     /locus_tag="ACHBRQ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9592131.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            20017..21708
                     /locus_tag="ACHBRQ_000404"
     CDS             20017..21708
                     /locus_tag="ACHBRQ_000404"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9592132.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            21824..22387
                     /locus_tag="ACHBRQ_000405"
     CDS             21824..22387
                     /locus_tag="ACHBRQ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9592133.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            22409..28078
                     /locus_tag="ACHBRQ_000406"
     CDS             22409..28078
                     /locus_tag="ACHBRQ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HFM9592134.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVMYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            28139..29860
                     /gene="pabB"
                     /locus_tag="ACHBRQ_000407"
     CDS             28139..29860
                     /gene="pabB"
                     /locus_tag="ACHBRQ_000407"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFM9592135.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            30391..31506
                     /locus_tag="ACHBRQ_000408"
     CDS             30391..31506
                     /locus_tag="ACHBRQ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HFM9592136.1"
                     /translation="MSKGDFIDPKTQFKHQYRGESPVFRIRNRKYVELGTTDKKLDER
                     RDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSVNSNLLNSFGEKAKKVLIRSKL
                     INVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDLTDGEYKYVAYESQQYTDPQTT
                     LTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPKPDEKPKPSAPQQEKTKPTVQS
                     GWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYVQNAWVGNYYLKSGGYMAKSEW
                     IYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAKSEWVYDSNYKSYYYLTSEGSY
                     ARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK"
     gene            31593..32512
                     /locus_tag="ACHBRQ_000409"
                     /pseudo
     CDS             31593..32512
                     /locus_tag="ACHBRQ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
     gene            32647..33606
                     /locus_tag="ACHBRQ_000410"
     CDS             32647..33606
                     /locus_tag="ACHBRQ_000410"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9592137.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            33703..34542
                     /locus_tag="ACHBRQ_000411"
     CDS             33703..34542
                     /locus_tag="ACHBRQ_000411"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9592138.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(34590..34760)
                     /locus_tag="ACHBRQ_000412"
     CDS             complement(34590..34760)
                     /locus_tag="ACHBRQ_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9592139.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            34881..35765
                     /gene="miaA"
                     /locus_tag="ACHBRQ_000413"
     CDS             34881..35765
                     /gene="miaA"
                     /locus_tag="ACHBRQ_000413"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9592140.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            35758..36996
                     /gene="hflX"
                     /locus_tag="ACHBRQ_000414"
     CDS             35758..36996
                     /gene="hflX"
                     /locus_tag="ACHBRQ_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9592141.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            36989..37612
                     /locus_tag="ACHBRQ_000415"
     CDS             36989..37612
                     /locus_tag="ACHBRQ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFM9592142.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            37627..38556
                     /gene="rnz"
                     /locus_tag="ACHBRQ_000416"
     CDS             37627..38556
                     /gene="rnz"
                     /locus_tag="ACHBRQ_000416"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9592143.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            38577..39332
                     /locus_tag="ACHBRQ_000417"
     CDS             38577..39332
                     /locus_tag="ACHBRQ_000417"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9592144.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(39386..40294)
                     /locus_tag="ACHBRQ_000418"
     CDS             complement(39386..40294)
                     /locus_tag="ACHBRQ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9592145.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            40358..40627
                     /locus_tag="ACHBRQ_000419"
     CDS             40358..40627
                     /locus_tag="ACHBRQ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9592146.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            40627..41007
                     /locus_tag="ACHBRQ_000420"
     CDS             40627..41007
                     /locus_tag="ACHBRQ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9592147.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            41096..41323
                     /locus_tag="ACHBRQ_000421"
                     /pseudo
     CDS             41096..41323
                     /locus_tag="ACHBRQ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(41363..42088)
                     /locus_tag="ACHBRQ_000422"
     CDS             complement(41363..42088)
                     /locus_tag="ACHBRQ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9592148.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            42250..44091
                     /gene="typA"
                     /locus_tag="ACHBRQ_000423"
     CDS             42250..44091
                     /gene="typA"
                     /locus_tag="ACHBRQ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9592149.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            44111..44365
                     /locus_tag="ACHBRQ_000424"
     CDS             44111..44365
                     /locus_tag="ACHBRQ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HFM9592150.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
BASE COUNT        13756 a         8126 c         9789 g        12764 t
ORIGIN      
        1 atttggaagg aagtttttcg taaccgccca ataacgaagt ctattggacg gttacgatat
       61 gatcactgac ttcgtcagtc ttatctacaa cctcaaaact gtgttttgag caacctgcgg
      121 ctagcttcct agtttactct ttgattttca ttgagtatta gaacagaaaa aatgcttgga
      181 gtatttgttt gtgtgtttat ttttatataa caaactataa acaaaacaaa aatataaaaa
      241 aagagacaaa aaagaacaaa aagtaattga caacggtttc atatagtgtt atacttatgg
      301 cataaagtta atggaaagaa gggaaaaact tatggtatta gattatttct ttgacaaaaa
      361 ccttgtgttt tgcttagaag cggataatca agaacatctc tttgatcagg ttgcaacttt
      421 attggaagaa cgagaaattg taactccaac ttatcgtgaa gccttgatca cgcgtgaaaa
      481 gtcatttcca actggtttag atatggaatt tctaggaaag gacttgccaa atgtagcgat
      541 tcctcacaca gatattgttc ataatctagc tgagaaagtg gtggttgttc gattagaaaa
      601 accagtaact tttcacaata tgatagctcc tgataaggaa gtagaagtat ctctactctt
      661 ctttatcatt aacaattcaa gttcaagtca aacgaacatt ctggctcagt tgatggactt
      721 tttcacagga aatggtcatc ttgaagacct atcaaaaatt tctgaaccag aaaaacttta
      781 tgcttacatt gctgaagcaa ccgcttaatc ttgtctatta aaaaataaaa tcggaggaaa
      841 tccaaatgat taaaattctt gctgcctgcg gtgcaggtgt taactcaagt caccaaatta
      901 aaagtgctct agaagaagaa ctttcaaacc gtggttatga tgttcactgt gatgcagtca
      961 tggtgaaaga tgtaaacgaa gaccttatga aaggttacga tatctttaca ccaatcgctg
     1021 caacagacct tggttttgaa ccaggtatcc cagttatcga agctgggcca atcttattcc
     1081 gtatcccagc aatgagcgct ccagtatttg acaatattga agcagctatt aaagaacatg
     1141 gattaagcta attctaattt gttaacattc atataacata aaaaagggag gaactgttat
     1201 ggatgcaatc tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc
     1261 tgtcatcatg ttgatcattt tgacagtatt ggctttactt tttggagtga aattctccaa
     1321 agcgcttgaa ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg
     1381 tatgctaaat ggtgctttct cagcatcact tgcaaaattc gttgaaaaca ctggtatcca
     1441 attgagtatt acagacgttg gttgggcacc actcgcaaca atcacttggg gttctgcttg
     1501 gacactatac ttcttgctca tcatgttgat tgtcaacgta gtgatgcttg ctatgaagaa
     1561 aaccgataca cttgatgtcg atatctttga tatctggcac ttgtctatca caggtctctt
     1621 gattaaatgg tatgctgata acaatggtgt gagtcaaggg gtttcactct ttattgctac
     1681 agcagctatc gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac
     1741 atttgatgac cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta
     1801 catgatgaac ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct
     1861 tgataaatat gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa
     1921 attcttcatc ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat
     1981 tgcaggtgtt gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg
     2041 tttgactgcc ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc
     2101 cgtagaacca ctatcacaag gtattacaaa cgttgctact aaacgtcttc gaggacgtaa
     2161 attcaatatc ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc
     2221 caacgtactt gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg
     2281 tatcttgcca cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac
     2341 tcgtggtaaa ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct
     2401 ttcaggtaca cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc
     2461 agaaggtgtg agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa
     2521 acttcttggt tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc
     2581 agtagtcttc cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa
     2641 acgtaacgaa gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa
     2701 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctaaaaacac agttttgagg
     2761 ttgtagataa gactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg
     2821 acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa gtcagtaatc
     2881 atatctacgg caaggcgacg ctgacgaagt ttgaattgga ttttcgcaga gtgtaaattg
     2941 ttgaaactag gttgtcatac catgtatacc acttgggctt tggtagtagc taactgcact
     3001 aaatataata taaggagggt tttatgggga aaggccattg gaatcggaaa agagtttata
     3061 gcattcgtaa gtttgctgtg ggagcttgct cagtaatgat tgggacttgt gcagttctat
     3121 taggaggaaa tatagctgga gaatctgtag tttatgcgga tgaaacactt attactcata
     3181 ctgctgagaa acctaaagag gaaaaaatga tagtagaaga aaaggctgat aaagctttgg
     3241 aaactaaaaa tgtagttgaa aggacagaac aaagtgaacc tagttcaact gaggctattg
     3301 catctgagaa gaaagaagat gaagccgtaa ctccaaaaga ggaaaaagtg tctgctaaac
     3361 cggaagaaaa agctccaagg atagaatcac aagcttcaag tcaagaaaaa ccgctcaagg
     3421 aagatgctaa agctgtaaca aatgaagaaa tgaatcaaat gattgaagac aggaaagtgg
     3481 attttaatca aaattggcac tttaaactca atgcaaattc taaggaagcc attaaacctg
     3541 atgcagacgt atctacgtgg aaaaaattag atttaccgta tgactggagt atctttaaca
     3601 atttcgatca tgaatctcct gcacaaaatg aaggtggaca actcaacggt ggggaagctt
     3661 ggtatcgcaa gactttcaaa ctagatgaaa aagacctcaa gaaaaatgtt cgccttactt
     3721 ttgatggcgt ctacatggat tctcaagttt atgtcaatgg tcagttagtg gggcattatc
     3781 caaatggtta taaccagttc tcatatgata tcaccaaata ccttcacaaa gatggtcgtg
     3841 agaatgtgat tgctgtccat gcagtcaaca aacagccaag tagccgttgg tattcaggaa
     3901 gtggtatcta tcgtgatgtg actttacaag tgacagataa ggtgcatgtt gagaaaaatg
     3961 ggacaactat tttaacacca aaacttgaag aacaacaaca tggcaaggtt gaaactcatg
     4021 tgaccagcaa aatcgtcaat acggacgaca aagaccatga acttgtagcc gaatatcaaa
     4081 tcgttgaacg aggtggtcat gctgtaacag gcttagttcg tacagcgagt cgtaccttaa
     4141 aagcacatga atcaacaagc ctagatgcga ttttagaagt tgaaagacca aaactctgga
     4201 ccgttttaaa tgacaaacct gccttgtacg aattgattac gcgtgtttac cgtgacggtc
     4261 aattggttga tgctaagaag gatttgtttg gttaccgtta ctatcactgg actccaaatg
     4321 aaggtttctc tttgaatggt gaacgtatta aattccatgg agtatccttg caccacgacc
     4381 atggggcgct tggagcagaa gaaaactata aagcagaata tcgccgtctc aaacaaatga
     4441 aggagatggg agttaactct atccgtacaa cccacaaccc tgctagtgag caaaccttgc
     4501 aaatcgcagc agaactaggt ttactcgttc aggaagaggc ctttgatacg tggtatggtg
     4561 gcaagaaacc ttatgactat ggacgtttct ttgaaaaaga tgccactcac ccagaagctc
     4621 gaaaaggtga aaaatggtct gattttgacc tacgtaccat ggtcgaaaga ggcaaaaaca
     4681 accctgctat cttcatgtgg tcaattggta atgaaatagg tgaagctaat ggtgatgccc
     4741 actctttagc aactgttaaa cgtttggtca aggttatcaa ggatgttgat aagactcgct
     4801 atgttaccat gggagcagat aaattccgtt tcggcaatgg tagcggaggg catgagaaaa
     4861 ttgctgatga actcgatgct gttggattta actattctga agataattac aaagccctta
     4921 gagctaagca tccaaaatgg ttgatttacg gttcagaaac atcatcagca acccgtacac
     4981 gaggaagtta ctatcgccct gaacgtgaat tgaaacatag caatggacct gagcgtaatt
     5041 atgaacagtc agattatgga aatgatcgtg tgggttgggg gaaaacagca accgcttcat
     5101 ggacttttga ccgtgacaac gctggctatg ctggacagtt tatctggaca ggtacggact
     5161 atattggtga acctacacca tggcacaacc aaaatcaaac tcctgttaag agctcttact
     5221 ttggtatcgt agatacagcc ggcattccaa aacatgactt ctatctctac caaagccaat
     5281 gggtttctgt taagaagaaa ccgatggtac accttcttcc tcactggaac tgggaaaaca
     5341 aagaattagc atccaaagta gctgactcag aaggtaagat tccagttcgt gcttattcga
     5401 atgcttctag tgtagaattg ttcttgaatg gaaaatctct tggtcttaag actttcaata
     5461 aaaaacaaac cagcgatggg cggacttacc aagaaggtgc aaatgctaat gaactttatc
     5521 ttgaatggaa agttgcctat caaccaggta ccttggaagc aattgctcgt gatgaatctg
     5581 gcaaggaaat tgctcgagat aagattacga ctgctggtaa gccagcggca gttcgtctta
     5641 ttaaggaaga ccatgcgatt gcagcagatg gaaaagactt gacttacatc tactatgaaa
     5701 ttgttgacag ccaggggaat gtggttccaa ctgctaataa tctggttcgc ttccaattgc
     5761 atggccaagg tcaactggtc ggtgtagata acggagaaca agccagccgt gaacgctata
     5821 aggcgcaagc agatggttct tggattcgta aagcatttaa tggtaaaggt gttgccattg
     5881 tcaaatcaac tgaacaagca gggaaattca cccttactgc tcactctgat ctcttgaaat
     5941 ctagtcaagt cactgtcttt actggtaaga aagaaggaca agaaaagact gttttgggga
     6001 cagaggtgcc aaaagtacag accattattg gagaggcacc tgaaatgcct accactgttc
     6061 cgtttctata cagtgatggt agtcgtgcag aacgtcctgt aacctggtct tcagtagatg
     6121 tgagcaagcc tggtattgta acggtgaaag gtatggctga cggacgagaa gtagaagctc
     6181 gtgtagaagt gattgctctt aaatcagagc taccagttgt gaaacgtatt gctccaaata
     6241 ctgacttgaa ttctgtagac aaatctgttt cctatgtttt gactgatgga agtgtacaag
     6301 agtacgaagt agatagctgg gagattacgg aagtagataa agccaaactt tcagtagctg
     6361 gatcacgtat tcaaatgact ggtcagttag ctggagaaac tattcatgca acccttgtgg
     6421 tagaagaagg aaatgctgca gcacctgtag tgccaactgt tactgttgga ggtgaagcag
     6481 taacaggtct tactagtcga caaccaatgc aatatcgtac tctatcttat ggtgcccaat
     6541 tgccagaagt cacagcaagt gctgaaaatg ctgatgtgac agttcttcaa gcaagcgcag
     6601 caaacggcat gcgtgcaagc atatttgttc agcctaaaga tggtggccct cttcaaacct
     6661 atgcaattca attcctagaa gaagcaccaa aaattgctca cttgagcttg caagtggaaa
     6721 aagctgacag tctcaaagaa gaccaaactg tcaaattgtc ggttcgagct cactatcaag
     6781 atggaacgca agctgtatta ccagctgata aagtaacctt ctctacaagt ggtgaagggg
     6841 aagtcgcaat tcgtaaagga atgcttgagt tgcataagcc aggagcagtc actctcaaag
     6901 cggaatatga gggagctaaa ggccaagttg aactcactat ccaagccaat actgagaaga
     6961 agattgcgca atctatccgt cctgtaaatg tagtcacaga tttgcatcaa aaacctactc
     7021 ttccaacaac agtaacggtt gagtatgaca aaggtttccc taaagctcac aaagtcactt
     7081 ggcaagctat tccgaaagaa aaactagact cctatcaaac atttgaagta ctaggtaaag
     7141 ttgaaggaat tgaccttgaa gcgcgtgcaa aagtctctgt agaaggtatc gtttcagttg
     7201 aagaagtcag tgtgacaact ccaatcgcag aagcaccaca attaccagaa agtgttcgga
     7261 catatgattc aaatggtcac gtttcatcag ctaaggttgc atgggatgcg attcgtccag
     7321 agcaatacgc taaggaaggt gtctttacag ttaatggtcg cttagaaggt actcaattaa
     7381 caactaaact tcatgttcgc gtatctgctc aaactgagca aggtgcaaac atttctgacc
     7441 aatggaccgg ttcagaattg ccacttgcct ttgcttcaga ctcaaatcca agcgacccag
     7501 tttcaaatgt taatgacaag ctcatttcct acaataacca accagccaat cgttggacaa
     7561 actggaatcg tactaatcca gaagcttcag tcggtgtcct attcggagat tcaggtattt
     7621 tgagcaaacg ttcagttgat aacttaagcg ttggcttcca tgaagaccat ggagttggtg
     7681 caccgaagtc ttatgtgatt gagtattatg ttggtaagac tgtcccaaca gctcctaaaa
     7741 accctagttt tgttggtaat gaggaccatg tctttaatga ttctgccaac tggaaaccag
     7801 ttactaatct aaaagcccct gctcaactca aggctggaga aatgaaccac tttagctttg
     7861 ataaagttga aacctatgct gttcgtattc gcatggtgag agcagacaac aaactaggaa
     7921 cgtctatcac agaggtacaa atctttgcga aacaagttgc ggcagccaag caaggacaaa
     7981 caagaatcca agttgacggt aaagacttag caaacttcaa ccctgatttg acagactact
     8041 accttgagtc tgtagatgga aaagttacgg cagtcacagc aagtgttagc aacaatggcc
     8101 tcgctaccgt cgttccaagc gttcgtgaag gtgagccggt tcgtgtcatc gcgaaagctg
     8161 aaaatggcga catcttagga gaataccgtc tgcacttcac taaggataag aacttacttt
     8221 ctcataaacc agttgctgcg gttaaacaag ctcgcttgct acaagtaggt caagcacttg
     8281 aattgccgac taaggttcca gtttacttca caggtaaaga cggctacgaa acaaaaaacc
     8341 tgacagttga atgggaagaa gttccagcgg aaaatctgat aaaagcaggt caatttaccg
     8401 ttcgaggcca tgtccttggt agtgatcttg ttgctgaggt cactgtacga gtgacagaca
     8461 aactaggcga agctctttca gataacccta actatgatga aaacagtaac caggcctttg
     8521 cttcagcaac caatgatatt gacaaaaact ctcatgaccg cgttgactat ctcaatgacg
     8581 gagatcattc agaaaatcgt cgttggacaa actggtctcc aacaccatct tctaatccag
     8641 aagtatcagc gggtgtgatc ttccgtgaaa atggtaagat tgtagaacgg actgttgctc
     8701 aagccaaact tcacttcttt gcagatagtg gtacggatgc accaactaaa ctcgttttag
     8761 aacgctatgt cggtccagag tttgaagtgc caacctacta ttcaaactac caagcctacg
     8821 acgcagacca tccattcaac aatccagaaa attgggaagc tgtgccttat cgtgcggata
     8881 aagacatcgc agctggtgat gaaatcaacg taacatttaa agctgtcaaa gccaaagcta
     8941 tgagatggcg tatggagcgt aaagcagata agagcggtgt tgcgatgatt gagatgacct
     9001 tccttgcacc aagtgaattg cctcaagaaa gcacgcaatc gaagattctt gtagatggaa
     9061 aagaacttgc tgatttcgct gaaaatcgtc aagactatca aattacctat aaaggtcaac
     9121 ggccaaaagt ctcagttgaa gaaaacaatc aagtagcttc aactgtggta gatagtggag
     9181 aagatagcct tccagtactt gttcgcctcg tttcagaaag tggaaaacaa gtcaaggaat
     9241 accgtatcca gttgactaag gaaaaaccag tttctgagaa gacagttgct gctgtacaag
     9301 aagatcttcc aaaactcgaa tttgttgaaa aagatttggc ctacaagaca gttgagaaaa
     9361 aagattcaac actgtatcta ggtgaaactc gtgtagaaca agaaggaaaa actggtaaag
     9421 aacgtatctt tacagcgatt aatcctgatg gaagtaagga agaaaaactc cgtgaagtgg
     9481 tagaagctcc gacagaccgc atcgtcttgg ttggaaccaa accagtagct caagaagcta
     9541 aaaaaccaca agtgtcagaa aaagcagata caaaaccaat tgattcaagt gaagctagtc
     9601 aaactaataa agcccagtta ccaaatacag gtagtgcggc aagccaagca gcagtagcag
     9661 caggtttagc tcttctaggt ttgagtgcag gattagtagt tactaaaggt aaaaaagaag
     9721 actagaaatt ttgatacctt ctttatcatt aaagaccaaa gaaaaactct tgtcctaaat
     9781 agaagataaa gagtctggaa caaaatagtt ctcaaccaca aaaaagctag atatttccaa
     9841 ttgcggaact ctagcttttt aattttgagt cgttctctta ttgtatagta gattgaaact
     9901 agagtagtac acttctactt ctaaaacatt gttagaaatc gatttgactg tccttatcga
     9961 tttgtcctgt tcttatttca tttcactatg ttttaggagg ttattggcca taagtaccaa
    10021 tcccatgtca attctcactt gacgcttgcc tctcagatta catctcttgt aacccaaaca
    10081 agcctttatc tgcccaaaga caggttctac atcaatcttg cgttgagcga aaatctgtct
    10141 atcttgggga gataaaagcg cctgacattc tttagctttc aagttttgat agcgttcgtt
    10201 catatacaat cccttttgag gggctgattc aggttcatca gcgtagtaaa ccttgatttc
    10261 ctgttgaaag tctgtctgtg ttttctggta cttgatatgg tgaaaacgat agcaccagcc
    10321 atcaggatgt atgtagctat cctccttgtc attatagtgc caattcgcta agtttttagc
    10381 tgactgttta taccctctct tttgttcctt atcaaacatg gcatatttaa tcagaagggg
    10441 agtgatttct tcttgaaaat agacttgtat ctgttcttga agtttggcgg agaatttctc
    10501 tgtcgttttc ttccacacaa aactatactt gttggcattg gattcaatct tagttccgtc
    10561 aataaacaaa caatctaagg tcactaactc ttccattttt caatttttcg accagagaaa
    10621 atcccttgtg aataggcaaa tagaagagta gatacaagca ttttagggtg ataagacggg
    10681 cgaccaaagg catgatagga ggtgtagaag tgacgttcct ccaaggtatt caccactttt
    10741 tcaatagtaa agacgagatg gtcttgtggt aagaaggaac tgatttctag tggtaaagtt
    10801 gtttgatttg tgttatagtg aatatgcatg ggatagcctt tctaaatggt ttattaagca
    10861 attctatttt accaagacta tcgtaaccat gcaaaaaagc aacggcaaat ccgttgcctt
    10921 ttttggatat aagagactat tgtcccagac tcttttcttt gtataaaatt gtatagtgtt
    10981 attgtctata ctattttgta gagaaagcaa aaatccctag aaaatgctta gggattcgac
    11041 tatttcatta agaacaattc aacaatcatc tctatataca tgataagggt taataaaaat
    11101 cctgtacgcc agaagaattt gatgaattta ggatagtaaa agctgcgttt ttttaaaagg
    11161 aagaaaaaga cgagaataat ggctaggaac gagagggcta gccccagtct agggaggaaa
    11221 ttatgggtaa aggttttagc tgtaatcaag taatactcaa ataccaagac tggaaaagcc
    11281 aaatccgcaa agtttcgtcc tagttttttt aatctaaaaa gtttggttag gataatgcag
    11341 acgactaagg tcagtatcaa taataaaata gatgctaatt tcattaaaat catacccata
    11401 ttgtatcata aaaaaaggct aatggaaacg agaaactgag gaatttatag gaaagtcagg
    11461 cttttgagat tgtgggtgat ttttgttata atgaaagtta taaaatctta tgataccttg
    11521 aatgattatt atcgaaaact ctttggagaa aagactttta aagtccctat tgatgcggga
    11581 tttgactgtc ccaatcgtga tggaactgtg gctcatggag gctgtacttt ttgtacggtt
    11641 tccggttctg gagatgccat tgtagcccca gatccgccta tccgtgagca attttataag
    11701 gaaattgact ttattcatcg taagtggcca gatgttcaga agtatctggt ttattttcaa
    11761 aattttacca acacccatga aaaggtggaa gttattcgag agcgttatga acaggctatc
    11821 aatgagccag gtgtggtagg aatcaatatt ggaacgcgcc cagactgttt accagacgaa
    11881 accatcgaat atttggctga gttatcggag tgcatgcatg tgacggttga attgggcttg
    11941 cagactactt atgaagcaac ctctgacctg attaaccgtg cgcattctta tgaattgtag
    12001 gtggaaacgg tcaagcgttt gagaaagtat ccaaaaattg agattgtttc ccatttgatc
    12061 aatggtttgc ctggtgaaac ccatgagatg atggttgaaa atgtccgccg ttgtgtcacg
    12121 gataacgata ttcaagggat taaactgcat ttgctccatc ttatgacaaa tactcgtatg
    12181 caacgagatt accatgaggg acgtttacaa ttgatgagtc aggacgaata tgtcagggtc
    12241 atctgtgacc aactggaaat tattcccaaa catatcgtca tccatcgaat tacaggagat
    12301 gcgcctaggg atatgctgat tggtcctatg tggagcctca ataaatggga agtcctcaac
    12361 agcattgaga tggagatgag acgtcgtgga agtgttcaag gatgcaaggc tgtaaaacag
    12421 gagtttgaaa atgaaaagac cacttgagat ggcacatgat tttttggctg aggtcgtgac
    12481 aaaagaggat gtcgtagtgg atgcgactat gggaaatggt catgacacgc tttttttagc
    12541 caagctagcc aagcaagtct atgcctttga tattcagaag caagccttgg aaaagaccca
    12601 agagcgtttg catcaggctg gcttgacaaa tgcccagtta atcttgcaag gccatgagac
    12661 actggaccag tttgtgacaa aagctaaggc agggattttt aatttgggct atttgccgtc
    12721 agctgataag tctgtcatca cccgaccgca gacaacgatt gaggcattag aaaagctatg
    12781 tggcttactt gtcaaaggtg gacgaattgc tattatgatt tactatggtc atgaaggagg
    12841 cgacctcgag agagatgctg tcttggattt tgtgatccag ttgaaccaac aagagtacac
    12901 agctgccatt taccgaactt taaaccaagt caacaacccg ccgtttttag tgatgattga
    12961 aaaattagag agatacagac atggataaac aatacctaca cgaaaagctg gatgccatgc
    13021 gccagaattt tgttgaatca acctaccacg aacgagcggt gggcgtgcta gaccaagcgc
    13081 atatgagcaa aaaaatgctt aaaatcaaga aaaaattagt tgctcttgaa atggaacggt
    13141 gccagagaaa aattgagcac aaagactgtt ccaagattga ccaaaaaatc aaagagcaga
    13201 aggagatatt tgaatcctgt tgtaaaaaag attaaggagg acgtgcgtgg aattactgat
    13261 ttacctcatc ctatttttac tggtcttgat tgtctcaagt acaaccaata agctcctacc
    13321 ctttttgcct ctccctttgg tgcagattct tttgggaatt gtgattggtc tctttttacc
    13381 caatactgac tttcacctta atacggagtt gtttttggca ctggttatcg gccccttgct
    13441 tttccgagag gctgaagaag cagatgttac ggctatttta aaacactggc gaatcattgt
    13501 ttatctcata tttccagtga tttttatctc gaccctgagt ttgggtggct tggcccatct
    13561 tctttggttc agccttccct tggcagcttg cttggctgtt ggggcagccc ttggtcctac
    13621 ggacttggtg gcctttgcct ctctttcgga gcgttttagc tttcctaagc gcgtgtccaa
    13681 tattcttaag ggcgaaggac tcttgaatga tgcttctggt ttggtggctt ttcaggtagc
    13741 tttgacagct tggacaactg gagctttttc tctggggcaa gctagcagtt cactcatctt
    13801 ttcaatccta ggcggttttt taattggatt tttaacagcc atgaccaacc gcttcctcca
    13861 taccttcttg ctaagtgtgc gtgcaacgga tattgccagt gaacttttat tagaattgag
    13921 tttgcctctt gtgaccttct ttctggcaga agaagttcat gtttcaggta ttattgccgt
    13981 cgtagttgct ggaattttaa aggcaagtcg tttcaagaaa atcacgctcc tcgaagccca
    14041 agtggatacg gtgaccgaga cggtctggca tacagtgacc tttatgctca acggttctgt
    14101 ctttgtgatt ttagggatgg agctggaaat gatagcagaa cctatcttga ccaatccaat
    14161 ctataatcct ctacttttat tgctatctct tatcgccctt acctttgtcc tctttgtcat
    14221 tcgttttatt atgatctatg gctattatgc ctatagaacc cgacgcctca agaaaaagct
    14281 aaataagtat atgaaggaca tgtttctctt gaccttttca ggtgttaagg gaacggtgtc
    14341 gattgctacg attctcttga taccaagtaa tctagaacag gagtatcctc tcttgctttt
    14401 ccttgttgca ggtgtgacgc ttgttagctt tttaacaggt ctcttggtct tgcctcatct
    14461 ttctgatgaa gaggaagaaa gcaaggatta tctcatgcat atcgccattt tgaatgaagt
    14521 aacgctagag ttggaaaaag agttggaaga caccagaaat aaactttccc tctatgcggc
    14581 tattgacaat tatcatggac gtattgaaaa tctcatttta agccaagaaa accaggatga
    14641 tcaagaagac tgggctgctt tgaaactctt gattcttagt attgaaagtg atggtttgga
    14701 acaggcctat gaagagggga atattagcaa tcgtgcttac cgagtttacc aacgttatct
    14761 gaaaaatata gaacaaggaa tcaatcgtaa acttgcctca agactgacct attattttct
    14821 tgtttccttg aggattttac gttttctcct tcatgaagtt tttactcttg gaaagacctt
    14881 ccgtagctgg aaggacaagg agcaaagccg tctccgtgct cttgattatg accaaattgc
    14941 agagctctat cttgccaata cagagatgat tattgaaagt ttggaaaacc tgaagggagt
    15001 ctacagacgc tctttgatta gttttatgca ggagtctcgt cttcgagaaa cagctattat
    15061 cagcagtggt gcctttgtcg aacgggttat caatcgtgtc aaacccaaca atatcgatga
    15121 aatgctgaga ggctattatc tggagcgcaa gttgattttc gaatacgaag aaaaacgatt
    15181 aattacaact aagtatgcca agaaattacg acaaaatgta aataacttag agaactattc
    15241 cttgaaggaa gctgccaata ccctgccgta tgatatggtg gaattggtaa gaagaaatta
    15301 gttaatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttggat tatatatgtg
    15361 actgacttcg tcagtttcat ctacaacctc aaagcagggc tttgagcaac ctgcggctag
    15421 cttcctagtt tgctctttga ttttcattga gtataagatt ataaatgaag gagtatgaca
    15481 tgaaaaagtg gtggaaagag ctgatagaca agcctttatt aaaagctttt ttgcattatt
    15541 atcaagcatc agatagtgag ttgaccagtg tcgcagtagc ctactattgg ttgatttcga
    15601 ttttccccct gcttttggtg gtggtcaata tcctccccta ttttcagatt cctgtgggag
    15661 aatttctgtg ctttatgaaa gacgttctgc ccccaagcct ctatgaaggt gtggaaaaaa
    15721 tagccagaga agttttgacg caaccttcaa caggtttatt gagtttttct gttttatcgg
    15781 cgttatggag tttttcaaaa tctatgaatt tcttgcaaaa agcttttaac aaggcttatg
    15841 gcgtcgaaaa gagtcgtgga cttatttccc atcagatgct aagtctccta gtcagttttg
    15901 gcttgcagct cctctttgcg tttgccttgt ttttgatctt atttggtcag atgattttgg
    15961 ctttacttgc tcattattgg acaaaagatg gcattatcta tcaggcttta caagggttgg
    16021 caggtcctct gatttacgcc ctcctctttg ctatcttggt catgctttat tattttcttc
    16081 ccaatatatc taacaggaaa attagctata ccctaccagg cagtgccttt gttctcttag
    16141 tgattttagg tttactaact ctgttctcta gttatctcaa ttactatgtc catcatttgg
    16201 tggatgtccg aattttaggt tccgttcttc ttgttgttat gatgttttgg tttatcttga
    16261 ttgctaaaat tgttatccta ggcgcggtta tcaatgccag tatgcagagt ttgagagatc
    16321 cggtctttaa aaaagactaa ctatttatcc attacaaaag cgcatagtat caaaacttta
    16381 tagaaccttg atactatgcg cttttgtgct ttgacagttt tttgtatagt ttttgtccaa
    16441 gatttctaaa tcatctgatt aaattcctat tttcccctat cattgtccct gttttttcga
    16501 aaatggaagg agtataataa atctatcaat aaaatgatag gaggaacttt atgggtcata
    16561 ttttcttttt tctaagtgtc tttttggcag ggattctatc cttcttttct ccttgtatct
    16621 tacctttgtt accggtctat acaggagtgt tactagatga taaggatggt gctcaggctt
    16681 ctagcggcaa attttcaatc tcagttacta gtttattacg aactctggcc tttatagcag
    16741 gaatttcctt tatatttatt ttgttgggct atggagctgg ttttttaggc gatttgcttt
    16801 atgcttcttg gttccaatat cttactgggg caattattat ccttcttggt ttgcaccaaa
    16861 tggagattct acactttaag gggctttata aggaaaagag gctacaactg caaggacagg
    16921 ggcaaaatgg taagggctat agtcaggcat ttttattggg cttgaccttt agttttgctt
    16981 ggacgccttg cgtggggccg gttctggggt ctgttttggc cttggcggct tcaggtggtt
    17041 caggagcttg gcagggagct ggtctcatgt tggtgtatac gctgggcttg gcgctaccat
    17101 tcttgcttct agctctgacc tctagttatg ttttgaaaca tttccgaaaa cttcatccct
    17161 atctcggaat cctcaaaaaa gtgggtggtt ttctcattat tgtgatggga ctcttggttc
    17221 tgtttggaaa tgcttcaatt ttaagtcaat tatttgaata aaatggaaag gaatatcaat
    17281 atgaaaaaat ggcaaacatg tgttcttgga gcaggttcgc tcctttgttt gacggcttgt
    17341 tcaggcaagt ccgtgactag tgaacaccaa acgaaagatg aaatgaagac ggagcagaca
    17401 gctagtaaaa caagcgcagc taaagggaaa gaggtggctg attttgaatt gatgggagta
    17461 gatggcaaga cctaccgttt atctgattac aagggcaaga aagtctatct caaattctgg
    17521 gcttcttggt gttccatctg tctggctagt cttccagata cggatgagat tgctaaagaa
    17581 gctggtgatg actatgtggt cttgacagta gtgtcaccag gacataaggg agagcaatct
    17641 gaagcggact ttaagaattg gtataaggga ttggattata aaaatctccc agtcctagtt
    17701 gacccatcag gcaaactttt ggaaacttat ggtgtccgtt cttacccaac ccaagccttt
    17761 atagacaaag aaggcaagct ggtcaaaaca catccaggat tcatggaaaa agatgcaatt
    17821 ttgcaaactt tgaaggaatt agcctaggag gtgtcttatg aatgataagt taaaaatctt
    17881 cttgttgcta ggagtatttt ttctagccat aaccggtttc tatgttctat tgatacgaaa
    17941 tgcagggcag acagatgcct cgcaaattga aaaggcggca gttagccaag gaggaaaagc
    18001 agtgaaaaaa acagaaatta gtgaagacgc agacttgcac gaaatttatc tagctggagg
    18061 ttgtttctgg ggagtggagg aatatttctc acgcgttccc ggggtgacgg atgccgtttc
    18121 aggctatgca aatggtagag gagaaacaac caagtacgaa ttaattaacc aaacaggtca
    18181 tgcagaaacc gtccatgtca cctatgatgc caagcaaatt tctctcaagg aaatcctgct
    18241 tcactatttc cgcattatca atccaaccag caaaaataaa caaggaaatg atgtggggac
    18301 ccagtaccgt actggtgttt attacacaga tgacaaggat ttggaagtga ttaaccaagt
    18361 ctttgatgag gtggctaaga aatacgatca acctctagca gttgaaaagg aaaacttgaa
    18421 gaattttgtg gtggctgagg attaccatca agactatctc aagaaaaatc caaatggcta
    18481 ctgccatatc aatgttaatc aggcggccta tcctgtcatt gatgccagca aatatccaaa
    18541 accaagtgat gaggaattga aaaagaccct gtcacctgag gagtatgcag ttacccagga
    18601 aaatcaaaca gaacgagctt tctcaaaccg ttactgggat aaatttgaat ccggtatcta
    18661 tgtggatata gcaactgggg aacctctctt ttcatcaaaa gacaaatttg agtctggttg
    18721 tggctggcct agttttaccc aacccatcag tccagatgtt gtcacctaca aggaagataa
    18781 gtcctacaat atgacgcgta tggaagtgcg gagccgagta ggagattctc accttgggca
    18841 tgtctttacg gatggtccac aggacaaggg cggcttacgt tactgtatca atagcctctc
    18901 tatccgcttt attcccaaag accaaatgga agaaaaaggc tacgcttatt tactagatta
    18961 tgttgattaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt
    19021 agggttactg acttcgtcag ttttatctac aacctcaaag ctatactttg agctgacttc
    19081 gtcagtttta tctacaacct caaagctata ctttgagctg acttcgtcag ttttatctac
    19141 aacctcaaag cggtactttg agaaacctgt ggctatcttc ctagtttact ttttgatttt
    19201 tattgagtat aagaggtctt tcctaagcag ttagaggaga attttgctat actgatacta
    19261 gtaagtggta aaggagcaga gcatgaccta cacaatctta atcgtagaag atgaatatct
    19321 ggtaagacaa ggtttgacta aactggtcaa tgtagcagcc tacgatatgg aaatcatcgg
    19381 tcaggctgaa aatggaaggc aggcttggga attgatccaa aagcaggtgc cagatatcat
    19441 tttaaccgat atcaacatgc ctcatctaaa tggcatccag ttggccagtc tggtacgaga
    19501 aacctatcct caggttcatt tggtcttttt aacaggttac gatgattttg attatgcctt
    19561 gtctgctgtc aaactaggtg tggacgacta cctgctcaaa cccttttctc gtcaggatat
    19621 tgaggaaatg ttggggaaaa tcaaacaaaa actagacaag gaagagaaag aagagcagtt
    19681 acaagattta ttgactaata ggtttgaagg aaacatggcc cagaaaatcc agtctcatct
    19741 ggctgatagc caatttagtt taaagtcttt agccagtgac ttaggtttta gtccgaccta
    19801 tctgagttcc ttgattaaga aagagttggg cttgcctttt caggattatc tggtgagaga
    19861 acgtgttaaa caagccaagc tcttgctttt aactacagat ctgaagattt atgagatcgc
    19921 agagaaggtt ggttttgaag atatgaacta ttttacccaa cgttttaagc agattgcagg
    19981 tgtgacacct cgtcagttta agaagggaga agaccgatga agcgttcttc tcttttagtt
    20041 agaatggtta tttccatctt tctggtcttt ctcattctcc tagctctggt tggaactttc
    20101 tactatcaat caagttcttc agccattgag gccaccattg agggcaacag ccaaacgacc
    20161 atcagccaga ctagccactt tattcagtct tatatcaaaa aactagaaac cacctcgact
    20221 ggtttgaccc agcagacgga tgttctggcc tatgctgaga atcccagtca agacaaggtc
    20281 gagggaatcc gagatttgtt tttgaccatc ttgaagtcag ataaggactt gaaaactgtt
    20341 gtgctggtga ccaaatctgg tcaggtcatt tctacagatg acagtgtgca gatgaaaact
    20401 tcctctgata tgatggctga ggattggtac caaaaggcca ttcatcaggg agctatgcct
    20461 gttttgactc cagctcgtaa atcagatagt cagtgggtca tttctgtcac tcaagaactt
    20521 gttgatgcaa agggagccaa tcttggtgtg cttcgtttgg atatttctta tgaaactctg
    20581 gaagcctatc tcaatcaact ccagttgggg cagcagggct ttgccttcat tatcaatgaa
    20641 aaccatgaat ttgtctacca tcctcaacac acagtttata gttcgtctag caaaatggag
    20701 gctatgaaac cctacatcga tacaggtcag ggttatactc ctggtcacaa atcctacgtc
    20761 agtcaagaga agattgcagg aactgattgg acggtgcttg gcgtgtcatc attggaaaag
    20821 ttagaccagg ttcggagtca gctcttgtgg accttgcttg gggccagtgt cacatctctt
    20881 cttgtctgtc tctgcttagt gtggttcagt cttaaacgct ggattgctcc tttgaaggat
    20941 ttgagagaaa ccatgttgga aattgcttct ggtgctcaaa atcttcgtgc caaggaagtt
    21001 ggtgcctatg aactgagaga agtaactcgc caatttaatg ctatgttgga tcagattgat
    21061 cagttgatgg tagctattcg tagccaggaa gaaacgaccc gtcagtacca acttcaagcc
    21121 ctttcgagcc agattaatcc acatttcctc tataacactt tggacaccat catctggatg
    21181 gctgaatttc atgatagtca gcgagtggtg caggtgacca agtccttggc aacctatttc
    21241 cgcttggcgc tcaatcaagg caaggacttg atttgtctct ctgacgaaat caatcatgtc
    21301 cgccagtatc tctttatcca gaaacaacgc tatggagata agctggaata cgaaattaat
    21361 gaaaatgttg cctttgataa tttagtctta cccaagctgg tcctacaacc ccttgtagaa
    21421 aatgctcttt accatggcat taaggaaaag gaaggtcagg gccatattaa actttctgtc
    21481 cagaaacagg attcgggatt ggtcatccgt attgaggatg atggcgttgg cttccaagat
    21541 gctggtgata gtagtcaaag tcaactcaaa cgtgggggag ttggtcttca aaatgtcgat
    21601 caacggctca aacttcattt tggagccaat taccatatga agattgattc tagaccccaa
    21661 aaagggacga aagttgaaat atatataaat agaatagaaa ctagctaact cccagtcaat
    21721 tctgggagtt ttatgcgtaa ttgggcaaac tttctaggtt gtctatcttg ctaaaaacaa
    21781 gaaaaaacgg tatgatagaa gagtgacaac agaggtaaca gagatgaacg agacacagat
    21841 tcaaagagaa acacgtcagg tagtggaaga tgttttagaa aagaccaatt tgaagcaggg
    21901 ggcccttttt gttttaggtc tatcttctag tgaggtgcta ggtggtcata ttgggaaaga
    21961 atccagtcaa gagattggtg agctgatagt ggagactatc ttggatattc taggtagtag
    22021 aggtatccat ttagcagttc aaggttgtga gcatgtcaat cgggctctcg ttgttgaacg
    22081 tcaggtggca gagcagtttg gtctggaaat tgtcagtgtc catcctactc ttcatgcagg
    22141 aggttcgggg cagttggcag cctttaagtt tatgcaggat ccagttgagg ttgaatttat
    22201 caaggctcat gctggattgg atatcggaga cactgcaatt ggcatgcatg tcaagcatgt
    22261 tcaggttccg attcgcccta ttttgagaga gattggtcat gcccatgtaa cggcactggc
    22321 tagtcgtcca aaattaatcg gaggtgcgcg tgcgcactat ccgcaagatg ctattagaaa
    22381 gacctgagaa tgaataaagg agataaaagt gtttaaaaaa gaccgttttt caattcgtaa
    22441 gattaaggga gttgtaggct ctgtatttct tggaagcctt ttgatggctc cttctgtagt
    22501 ggacgcagcc acctatcact atgtaaataa agagattatt tcacaagaag ctaaagattt
    22561 aattcagaca ggaaagcctg acaggaatga agttgtatat ggtttggtgt atcaaaaaga
    22621 tcagttgcct caaacaggga cagaagcatc tgttttgaca gcttttggtt atctatcagg
    22681 tgacatcctc aaaacgcttg gcttggacac tgttttagaa gaaacctcag caaaacctgg
    22741 agaggtgact gtagtcgaag ttgagactcc tcaatcaaca acaaatcagg agcaagctag
    22801 gacagaaaac caagtagtag agacagagga agctccaaaa gaagaagcac ctaaaacaga
    22861 agaaagtcca aaggaagaac caaaatcgga gataaaacct actgacgaca cccttcctaa
    22921 agtagaagag gggaaagaag attcagcaga accagctcca gttgaagaag taggtggaga
    22981 agttgagtca aaaccagagg aaaaagtagc agttaagcca gaaagtcaac catcagacaa
    23041 accaacagag gaaccaaaag ttgaacaagt aggtgaacca gtcgagccaa gtgaagacga
    23101 acaggcacca acggcaccag ttgagccaga aaagcaacca gaagctcctg aagaagagaa
    23161 ggctgtagag gaaacaccga aaccagaaga taaaataaag ggtattggta ctaaagaacc
    23221 agttgataaa agtgagttaa ataatcaaat tgataaagct agttcagttt ctcctactga
    23281 ttattctaca gcaagttaca atgatcttgg acctgtttta gaaactgcaa aaggtgtcta
    23341 tgcttcagag cctgtaaaac agcctgaggt aaatagtgag acaaataaac ttaaaacggc
    23401 tattgacgct ctaaacgttg ataaaactga attaaacaat acgattgcag atgcaaaaac
    23461 aaaggtaaaa gaacattaca gtgatagaag ttggcaaaac ctccaaactg gggttacaga
    23521 agctgaaaaa gttgcagcta atacagatgc taaacaaagt gaagtaaatt ctgagactgc
    23581 aagtcttaaa acagcaattt ctagattaaa tacagataaa gttgaactcg aaaatcaact
    23641 taagattgca cagggaaaaa ctgaaactga tttcagcatg gaatcttgga ctgttttgag
    23701 tacagctaaa aataaagctc aggaagtaaa agataacgga acagcgaccc aagaacaaat
    23761 aaacgaagca gagaaaagtt tgaaaacagc ccttgctgac ttatctgttg acaaaacagc
    23821 tttgggaagc gctatcgata cagctactaa gaaaaataaa gagaattata caaatcaaac
    23881 atgggcagag ttggaaacag cgcttacagc agcaaaatct gtaaatacta acgagtctaa
    23941 acaaagcgac gttaacgaag ctgctgaaaa attaactgca actatggaaa aattggttga
    24001 attgtctgaa aagccacgtt tgactctatc tattgaaaag agagacatag atcgaaaagc
    24061 tacggtcacg tatactttgg aaaatccagc aaatactcaa ataaaatcaa taactgcaac
    24121 tctgaaaaaa ggagaggaag ttgttaagga ttttgtttta acagaagaaa atcttaaaac
    24181 taatcattta actgctttgt ttgaaaagct agactactac aaagaataca ctctatctac
    24241 ggatatggta tataacagag gaaatgatga tgagacggag agtatttcag aagaactaat
    24301 tcagctgaat cttaagaaac tagaattgaa agatatccag acagtctctc tgatgaaatt
    24361 tgaaaatggt caagaaagtc aggttacaca cttgtctgat aaaccgaccg atttgagtaa
    24421 attatactta aaagttacat catcaacgtc taaggatgct gtgttagccg ttagctctat
    24481 agaagaagag attattgaaa ataaaaaaat ctttaaaatt catgcagata caccagaatt
    24541 agtagttcgt aagaaagatg gcagtctttc taagggattt gactattaca tggagagagt
    24601 tatccctcat gatggtgata tttattatga ttttaaagac ttgatttcag ctatgacatc
    24661 taatcctaca ggtacgttta ttttagggag agatatttcc tcgcgtaatg taaaaccgga
    24721 tggaaatgga aaatcttata tcaaaggtga atttaaagga aaactacttg gtacaaatga
    24781 taatgttaga cactcgatct ttgacttaga atatccattg tttgatacta taaaatcagg
    24841 agtcgtgaaa gatattgatt tcaagcatgt caatatggta ttcccagata gcaatcaagg
    24901 agataacgta gcaacaattg caagagtaat caaagataag acaaaaattg agaatgttaa
    24961 tgttgaaggt tatctcgagg gtcgagatca tgtagctggt ttagttaata atcttgaagg
    25021 taattccgaa attgaaaatg tatcctttac aggaaagatt aagtctaaag gtggtaattc
    25081 tattacagca ggtattgcag gtagaaatat cttatctcgt gttaaacgtg cctatgtaaa
    25141 tgcagatata gaggtacatc gatcatctaa ttctagcatg cttgttgcag taaatggaat
    25201 aaacgcagat gcaagtggtg gttggggaac ctggggtaga cttactgagt cagttgctaa
    25261 aggaacatta gaaaccaagc aaggagggca agctggagga gcctcttcta cagtctggcc
    25321 ttatggagca attgataatg ttgtcagcta tgcaaaagtg accaaaggga aagaattatt
    25381 tggatcagat ggtgatttaa attatgattg gtttatgaaa aaaattagta atatttttgg
    25441 tgttcaaggc atcagctcag gcgattcggg tagtgattct aaatttacgc gtatttctga
    25501 ggaagaagca aatcaaaaag ttgcttctta taatattaca gctcccaact tgatgagtga
    25561 ctcaagtctt ttggtagaca gactcaatga aagttggaaa aatactgatc aatttgaaag
    25621 tatacaggat tatcaagccc aaaaccaatt aatttaccag aacttgacta aatttactcc
    25681 ttattacaat aaagaattta ttgttcatga aggaaatgct ctcactcctg aacaggaaat
    25741 attgaagact aagaaaatca aatcgattgt agggttgaaa ggaacagagt tcgttgtaga
    25801 tggcagtgat attgatacta tcatgcttca ttttgaggat ggtagtcaga agcgatataa
    25861 ggtaacctct actggtaagt tttcgataac taatttaccg gaatatcagg ttgaagattt
    25921 aaatgtgatg tatacatcag aacatatagt acatccacta gattcgtcac tgataaataa
    25981 ccttgttgaa gaattgaaaa aagtagaatt gtatacagag tctacttacc aagttctagg
    26041 tattgataaa gataatgcta acaaattgaa tagaactaag agattattct tagatgagtc
    26101 attagatgct gttaaaacac aattgcctac ctttgtaaaa acaatgtttg aaaatgagtg
    26161 gttgcacatc aatggagaat ctagtggagc tgtcgctgct ttacgacaaa aaatcatgga
    26221 taataagacg gctatattgc ttgctttgac ttatatcaat cgttattatg atgttaaatt
    26281 ctcagactat aatattaaaa aactgatgct ctttaaacca actttccatg gtgaaaagat
    26341 tgatctatta gatcgattaa tccgactagg atcatctgga gaaaatagat taaaaggttc
    26401 agaaaatgca gaaacgttca agcagttatt tgctagcgaa acgaagcaga aggatttggt
    26461 gacttattta gactacaatc gctctttgtt gactaattat caaacaacag gggaatggtt
    26521 taaagaaacg actaaggatt atattcaatt tgaggaacgt ccatccttag tagaggagat
    26581 taaagatgcc aaatatcgag tttacgataa tttaaccgca ccgtattatc aaggatatat
    26641 cctacctctc ttaacgttga agaatactca tttagcaatt ttatcgaact atagtactat
    26701 gacatttgtc agccgtgaaa aacgccctaa ttggaaaaat gaagattttg ataagtgggt
    26761 gaaatatgta gcaactgctc atagaaacca tgttgatact tggtataaga ttttacctga
    26821 taatatcaaa ggtaaaatgg ttaaagagaa tgttactgct gtttgggaag gattgagcat
    26881 cccaggaagt gaatgggtag accagaatgc tgtagataga aaaggaagag attacgcccc
    26941 agctcgagaa ttctttaatc ttgtaggtgg tcctatggga ggttggtatg cttaccatgg
    27001 ttatggtgcg catgctggag gaagaaatcg ggttaattat gaggtgtttg atgtattaag
    27061 tgagtatggt atatcggtct ttactcatga attgactcac gttaatgata cttggattta
    27121 cttaggaggg tatggtagac gtgaaaatat gggacctgaa gcttatgctc agggattatt
    27181 ccagtctcct gttccaggtc agccgggttg gggggcgctt ggtctcaata tggcatttga
    27241 aagaaaaaat gatggtgatc taatttataa tgcctcccca actcaatttg aaaataggaa
    27301 agagttagat agttatatga aaaattataa cgatactctc atgatggtgg actacttgga
    27361 aggagatgca gttatctcta aaggtaaaga agctattact aaatggttta aaaaagttga
    27421 acctaaggtt gttagtcaaa cagctcagta tgatacggtt cgtcagttga ctgctgaaga
    27481 gaaagaaaaa ctttctgttt ctagtgttga tgatttagtt gatcaaggat taatgtcaga
    27541 cagagctgta ggaaataata cctataatcc agctgatttt gaaacaagtt atattgcgat
    27601 agattatatg actggtattt atggtggagg aaagaatagt gtaggttcac caggtgcctt
    27661 gatgtttaaa cataatactt tccgtatgtg gggctactat ggttttgaag aaggtgttct
    27721 aggatatgct tctaataaat ttaagcaagc atctagagat gaaggtcatg ctggacttag
    27781 tgataacttt attatttcta agatttccaa aggggaattt ttaactatgg aagcatttaa
    27841 aaagggatat tttaagaaag tagtagaaga gttaaaaact aaaggtattc gtcctgttac
    27901 tatcaatcaa aaaacatata gcacctttga agaattgcaa gaaggattta agcaggctgt
    27961 tgaaagagat ttgaagaaaa atcagttaga tgagagggaa acaagaaact tcaaattcca
    28021 agttttcaga caacttcttc aacaaacaga tagctttaaa acttctatct ttagataatc
    28081 gaaatctctc atctgctttg cggatgaggg ttttcttatt ttatgctata cttaaagtat
    28141 gcatagaaaa acagtgattg attttaggac tttgggggag agatatacct ttacccagcc
    28201 tattaaagag ttgaaaacga gaaatgtaga agaagtggca gatttgctgg cacaagtgga
    28261 aagctaccaa gagcaaggtt attatgtggt ggggtatgtc agctacgagg ctgcacctgc
    28321 ttttgaggaa aaattagcag ttcacaagga tcccctactg ggagagtacc tgctttattt
    28381 tactgttcat gatagcgtag aaacttcccc tattcctctg acttatgagg atattgattt
    28441 gccctcaaat tggcaggaag taacgtctgc agcagactat gaaaaggcca ttgcccaaat
    28501 tcaccatcat ttacggcagg gagataccta tcaggtcaac tacaccgtcc aacttaagca
    28561 aaagttaaat gccaatcctt ttgccatcta caatcgtatg gtggtagagc aggaggcggg
    28621 ctacaatgcc tatgtggaac atgacgagat ggcagtgatt tccatgagcc cagagctctt
    28681 ttttgagcaa aatgatcgcg agttgacaac acgaccaatg aaggggacga ctcagcgtgg
    28741 ggtaactgac caagaagatc ttgaacaggc cagttggttg gaacaggatc ccaaaaatcg
    28801 ctctgaaaat atgatgattg tggacctctt gcgcaatgat atgaaccgta tttctgaagt
    28861 tgggagcgag cacgtggagc gtctgtgtca ggtagagcag tattcaactg tttggcagat
    28921 gacttcgacc atcaagagtc agttgcgaga ggatgtggac cttgttgaaa tcttccactc
    28981 actctttcct tgtggttcca taacgggtgc accgaaaatt gcgacaatgg agattatcaa
    29041 ggacttggag cctcaaccgc gtggagtcta ctgtggaacg attggtctct tgcttccaaa
    29101 tggacgacgg atttttaatg tggccattcg taccattcaa cttcacaaag gtcaagccat
    29161 ttatggagtt ggcggaggga ttacttggga tagcacttgg gaatctgaat accgtgaagt
    29221 tcatcaaaag gcagcagttc tctatcgtaa acaagctcgc ttccaattaa ttacaactgg
    29281 aaaaatcagc caaaaacaac tgatgtttga agaacaacat ctggaaaggc tgagaaaagc
    29341 tagtcggtat tttgcttatc cttttgaccc agaaattttg agacaaaaga ttgaagcaga
    29401 gtgtcagact tgtgatgcta atcaagatta ccgcttgcga atttctctca gcaagtctgg
    29461 agagatagaa ttcagtcgtc aaatcttaac acctcttagt ccaagttttt gtcaggccaa
    29521 actttgtctg caagaagcga atttgaatca atcctttacc tattttaaaa cgacttacag
    29581 accgcatttg agcatagagg aacaagagaa gatttaccat aatggaaaag gagaacttct
    29641 tgaaacctct atcggaaatt tggttctgaa aatcgatggg aaactctaca caccgcctat
    29701 ccgacttgga atcttgccag gaatttatca ccagtattta ctagaaacag gacaggtaga
    29761 agagaaagtc ttgaccttgg cagattttgc ccaagcagaa gctatttacg gctgtaatgc
    29821 agtgagaggc ttgtatgagt tgcaagtgat aggattgtga gttttagcta ttaatagttt
    29881 ttaatatata ttaaaatata ttggtgttta cttttgatga gatatcatat ttgtgttata
    29941 atgtaaccgt aaacaataga aatttaaagg agatttttga tgaaatcaaa aatagttttg
    30001 ggagcttcgc tagcgatcgc aacacttagc ttagtatctc ttgtcgagat tgaaggactc
    30061 tcaccgtttt taatagagaa tgttagcgca aatactcatt ctgcaaataa agttattaat
    30121 cataaagtaa gtatttactt agaaaatgct gatgaaggta aaggtctaac tgtaaacttt
    30181 tcaacagaca gtgttagtcc aaatttattt gatgagttcg aaaaaaagag tggaattaca
    30241 ataactacta tgttagtgaa tgctaagaca ggtgaagttg tagaaaaaag attaacacca
    30301 agcgtttttt tacgttccaa tgatttaacc agtgggacca tatcttcgtt tatttttagt
    30361 gaatatcctg atggagaata taaatatgtt gtgtctaaag gagactttat cgatcctaag
    30421 actcagttta aacatcagta tagaggagaa tctccagttt ttagaatacg taatcgaaaa
    30481 tatgtggagt tgggaacaac tgataaaaaa ttagatgaaa gacgtgataa tagcgtttat
    30541 aaagatgggg ttgttgaaca caaggtaaat ttaagtttaa caagttatca aggaggtaat
    30601 ggagttactg caattttttc aactgatagt gttaactcta acttacttaa ttcttttggt
    30661 gaaaaggcta aaaaagtttt gattaggtct aagttaatta atgttaagac tggcgaggtt
    30721 attgatgaaa cgttttctcc aaaagtaagt ttaacctcta aaattttgaa aagtggttct
    30781 acagcagtat tttattttat agatttaact gatggcgaat ataaatatgt tgcgtatgaa
    30841 agccagcaat atactgatcc tcaaacaaca ttgactcatc agtatagagg agaatctcct
    30901 atttttagta tcaaagatgg aaaattttct ggtttagtat ctgcctcaaa accagacgaa
    30961 aacccgaagc caacgccaaa accggatgag aaaccaaaac cttcagcacc acaacaggag
    31021 aaaacaaaac ctacagttca gtctggttgg gtaggttcat cttattatca aaatggtaaa
    31081 aaagtcacta gtaagtggat ttttgataaa aaatataatt catacttcta tctagatgca
    31141 tcaggaaatt atgttcaaaa cgcttgggta ggtaactatt atctcaaatc aggcggatac
    31201 atggctaaaa gtgagtggat ttatgataag aactatggtt catactacta tctaacatca
    31261 gaaggcagct acgctcgtaa cacttggtca ggcaattatt atttgaagtc aaatggtaag
    31321 atggctaaaa gtgagtgggt atacgatagt aactacaaat catattacta cttgacatca
    31381 gaaggaagtt atgctcgtaa cacttgggta ggcaattact atcttaaatc aaacggtaaa
    31441 atggctgtta atgaacgtac accagatggt tatcgtgtag atggttcagg taaatgggtt
    31501 aaataagaaa ctaaaaaata ggataatagg taatatcaga tgaataaaaa aaattatttt
    31561 catgtgcttt agcattttca acgattggaa cattgtcaac gattgataaa tcaggagttc
    31621 ttcctttttc agcgcaaacg gtgcaggctg acatacaaac gcttacccca atggcaaaat
    31681 attatattat tcttccgaag gatgcagaaa tatataaaac ttggagaggt actgtcaata
    31741 ttcccattat tgatgctaca aaaactactc cagaattatc ttatttcaaa gaagatcatc
    31801 gcaactatat agctaatgag aataagtctg gtgccaatta catagagtgg aagggaacag
    31861 tagaagagtt taaagaggcc attaagaagt tgactgataa aaaatcaact actgcaacac
    31921 caaaaaagga tgaaaagcca acaccaaaac cggatgagaa gccaaaacca acacctacag
    31981 ttcagtctgg ttgggtaggt tcatcctatt atcaagatgg taaaaaagtc attagtaagt
    32041 ggatttttga taaaaaatat aattcatact tctatctaga tgcatcagga aattatgttc
    32101 aaaacgcttg ggtaggtaac tattatctta aatcaggcgg atacatggct aaaggtgaat
    32161 gggtttatga tgccacctat caagcttggt attatttgac atcagatggt tcttatgctt
    32221 acagtacatg gcaaggaaat tactatctaa aatcggatgg taaaatggct gtcaatgaat
    32281 gggttgatgg tggacgttat tatgttggcg ctgacggagt ttggaaggaa ggtcaagcaa
    32341 gtacagcttc ttctagtaat gatagcaata gtgaatattc tgctgcttta ggaaaggcaa
    32401 aaagttataa ttcgttattc cacatgtcaa aaaaaaacgt atgtatagac aattaacttc
    32461 tgattttgat aaattttcaa atgatgcagc tcaatatgcc attgatcatt agatgattaa
    32521 cagatactgg taggcgaaaa aattccttat aattcattta cgcaaacctt tgcataagtg
    32581 aatggatttt gatatactag tactgtaagc attttcaatt actacacaat gaaaaaggag
    32641 aatatgatga gtcaaaagat tattgggatt gaccttggtg gaacttctat caaatttgca
    32701 atcttaacaa cagcaggaga aatccaagga aaatggtcaa tcaagaccaa cattttggat
    32761 gagggaagtc atatcgttga tgatatgatt gagtctattc aacatcgttt ggacttgctt
    32821 ggattggcag cagcggactt ccaaggcatt ggaatgggat caccaggtgt ggttgaccgt
    32881 gacaaaggga ctgttatcgg tgcctacaac ttgaactgga aaacccttca accaatcaaa
    32941 caaaagattg aaaaagcttt gggcattcca tttttcatcg ataatgatgc caacgtagca
    33001 gctcttggtg agcgctggat gggtgctgga gataaccaac cagacgttgt ctttatgaca
    33061 ctcggtactg gtgttggtgg cggtatcgtc gcagaaggca aattgcttca cggtgttgct
    33121 ggtgcagcag gtgagcttgg tcacatcact gttgactttg accagccaat ctcatgtact
    33181 tgcggtaaga aaggctgcct tgagacagtt gcttcagcaa cagggattgt caacttgact
    33241 cgtcgctatg ccgatgaata cgaaggcgat gcagccttga aacgcttgat tgataatgga
    33301 gaagaagtaa ctgctaagac tgtctttgat ctcgcaaaag aaggagacga ccttgctttg
    33361 attgtttacc gtaacttctc acgttacttg ggaatcgctt gtgctaacat tggttcaatc
    33421 ctaaacccat caacaatcgt catcggtggt ggtgtgtcag ctgcgggaga attccttcta
    33481 caaggtgttc aaaaagttta cgatgaaaat agtttcccac aagtacgcac atctactaaa
    33541 ttagctcttg caactctagg aaatgacgct ggagttatcg gagcagcatc acttgtattg
    33601 caataagata atgagagagg aaaatgctaa cctagagtta gtaatgttcc tcttttattt
    33661 tttatgctat actagttagg acaatgaatg aggtgagagt aaatgacaaa agcagatacg
    33721 atttttaaag agaatattga atgcattcta aaggaaggtg ttttctcaga gcaagctcgt
    33781 cctaagtaca aggatgggac tgttgctaat tccaagtatg taactggtgc cttttccgag
    33841 tacgacttat ctaaaggaga atttcctatc acaaccttgc gtcccattgc aatcaaatcc
    33901 gccatcaaag aagttctctg gatttaccaa gaccagtcaa atagcttaga agtgcttaat
    33961 gacaagtaca atgttcacta ctggaatgac tgggaagttg gagacacggg aaccattggt
    34021 gagcgctatg gtgccgttgt taagaaacac gacattatca ataagcttct caaacagttg
    34081 gaaaccaatc cttggaatcg ccgcaatatt atttcgctct gggattacca agctttcgaa
    34141 gaaacagatg ggctgctccc gtgcgccttt cagaccatgt ttgatgtccg gcgtgttgat
    34201 ggggaaatct atctggatgc gaccttgacc cagcgctcca atgatatgct ggtggcccac
    34261 cacatcaacg ctatgcagta tgtggcttta cagatgatga ttgccaaaca ttttggttgg
    34321 aaggttggga agttcttcta tttcatcaac aacctccata tctatgataa tcaatttgaa
    34381 caagctcagg aattgctccg tcgggagccg tcaaactgcc aaccacgctt ggttttaaat
    34441 gttcctgatg ggactaattt ctttgatatc aaagcagaag attttgagtt ggtggattat
    34501 gaccctgtta agccacagtt gaagtttgac ctagctattt aaaagaatag aaaaaagaag
    34561 ttgagaataa tcccaacttc ttttgtttct taacgtgata cgcggcgacg agctgctttt
    34621 ttacgatttt cttcgatgaa agctgctttt tgctcttctg gttcgattac tttcttttta
    34681 aatgcgtata ctgcacctgc aacggcagcg acagttcctg cgacacctgt tacaagacct
    34741 ttagcgaatc ctttagccat gagtcttcct cctttatatt ctcaatcagc cagcctcctc
    34801 aagaggtcac atttttctga ctgacctttt tgtgttataa taatagtaac gaaaaaatgg
    34861 gaatttttca aggaaaaaag atgaaaacaa aaataattgt gattgttgga ccgactgctg
    34921 ttggaaagac ggctctagct attgaagttg caaagcgctt taatggcgaa gtggttagtg
    34981 gagatagcca gcaagtctat cgaggacttg atattgggac ggccaaggct agtccagaag
    35041 agcaggcagc tgttcctcat catttaatcg atgttagaga gataaccgag tcttactcgg
    35101 cttttgattt tgtttcagaa gctaagatga ctattgagga tattcagagc cgtggcaagc
    35161 tagccattat cgccggtggg actggacttt atatccagag cttgctagaa ggttaccacc
    35221 taggtgggga gactcctcat gaggagattt tagcttatcg agctagtttg gagccatatt
    35281 cagatgagga attagcccat ttggtggagc aagcaggcct tgagattccc cagtttaatc
    35341 gtcgtcgtgc tatgcgtgcc ttagaaattg cccattttgg tcaggatttg gaaaatcaag
    35401 agatcttgta tgaaccgctg attatctgct tggatgatga acgtagtcaa ctttatgagc
    35461 gtatcaacca ccgagtggat ttgatgtttg aggctgggct tttggatgag gccaagtggc
    35521 tgtttgacca ttcccctaat gtacaggctg ctaaaggaat tggctataag gaactctttc
    35581 cttatttccg tggagagcag acctttgagg aagctcgtga gagtcttaaa caggcgaccc
    35641 gtcgttttgc caagcgtcag ctgacctggt tccgtaatcg catgcaggtc accttttatc
    35701 agattggaga atctggtgta caagaccgca ttttaagcca gatagaggag tttttagatg
    35761 attgaaacgg agaaaaaaga ggagcgagtc ctgctgattg gtgtggaatt gcagggtatg
    35821 gacagttttg acctctccat ggaagaattg gctagtttag cgaaaacggc aggggcagtc
    35881 gttgtagata gctacagaca aaaacgtgaa aaatatgatt ccaagacctt cgtcggctct
    35941 ggtaagttgg aagagattgc gcttatggtg gatgcagaag aaatcactac tgtcatcgtc
    36001 aacaatcgtc tgaccccaag gcagaatgtc aatctagagg aagttctcgg tgttaaggtc
    36061 attgaccgta tgcagttgat tttggatatc tttgccatgc gggctcgaag ccatgaaggg
    36121 aagctccaag tccacctagc ccaactcaaa tacctcttgc ctcgcttggt tggtcagggg
    36181 attatgctca gccgtcaggc agggggaatt ggttcccgtg gtcctggtga aagccaactg
    36241 gagctgaacc gtcgtagcgt tcgcaatcaa atcacggata tcgagcgcca gcttaaggtg
    36301 gttgagaaaa atcgtgcgac tgtcagagaa aaacgtttgg agtctagcac ttttaagatt
    36361 ggtttgattg gttataccaa tgctgggaaa tcaactatca tgaacatctt gaccagtaag
    36421 acccagtatg aagcagatga gctctttgcg actctggatg cgacaaccaa gagtattcat
    36481 ctgggaggca acctccaagt aactttgaca gataccgttg gctttatcca agatttgccg
    36541 acagagttgg tgtccagttt caagtcaacc ttggaagaaa gcaagcatgt ggaccttctg
    36601 gttcatgtta tcgatgctag caatccttac cacgaggagc atgaaaaaac ggttctctcc
    36661 atcatgaaag acctggacat ggaagatatt cctcacttga cgctttataa taaagcggat
    36721 ttggtggagg atttcacgcc tacccaaacg ccatataccc tcatttctgc caagtctgag
    36781 gacagtcgtg aaaacttgca agcattattg ctagataaga ttaaggaaat ttttgaagca
    36841 tttaccctgc gagtgccttt ttcaaagtcc tacaagattc atgatttaga gagtgttgca
    36901 attctggaag aacgtgatta tcaggaagac ggcgaagtga ttacaggcta catttccgag
    36961 aaaaataaat ggaggttaga agaattttat gactgatatt aaaactttgg ctctaaagta
    37021 tggaggttat acaagtctgg acaaggtcta tctggatcag cttctagctg gtaaaacaga
    37081 tcaggagcag ttaacactta ttacacctcc gccaagtgtt gttaatgcct actttgcaga
    37141 actctatcag aaaaagagtc ctcaggctgc gacggattat tttgcagaac tcagtcagga
    37201 actgaatctt tacaatactg agccaagttt caccctagaa aataagcctt ttattcggct
    37261 taatctgtct ggtaaatcct ttggtttttg ttatgagagt gacggccttg gtcggatttt
    37321 ctttgaaaat gaggagaaaa tctcggatga cttgctcttt gaaattgcgc aaattttccc
    37381 ccatcaatta gtctttgaag agtctggtaa gatttacatg aagcctgtcg aggacgagga
    37441 agttatcagc ctagaaagtc tcacagcttt gactgatttg gaaagattgg ctgatggtcg
    37501 aaaacgtctc aaaggctaca gccaagagga tttattgcaa gaagctgctg ctttttctgg
    37561 caaacgctat ttccgatcgg aaaaccgcac agccatgtta tatattgatt aattagaaag
    37621 tatcgaatgg atattcaatt tttaggaacg ggggctggtc agccctctaa agcccgcaac
    37681 gtttcaagtc tcgccctgaa acttttggat gagattaacg aagtttggct ctttgactgt
    37741 ggagaaggta cgcaaaatcg cattctggaa accacaattc gaccacgtaa ggtcagcaaa
    37801 atctttatta cccatctgca tggagaccac atttttggtt tgccaggttt cctttctagc
    37861 cgtgcctttc aggccaatga agagcagaca gatttggaaa tctacggacc tcaaggaatc
    37921 aagtcatttg tcttaaccag ccttcgtgtg tcaggttctc gtctgcccta ccgcattcat
    37981 ttccatgagt ttgaccaaga ttctctgggt aaaattcttg aaaccgataa attcactgtg
    38041 tatgcagagg agctggacca cactattttc tgtgttggct atcgtgtcat gcaaaaggat
    38101 ctagaaggga cgctggatgc tgaaaaactc aaggctgctg gtgttccgtt cggcccgctt
    38161 tttggtaaaa tcaaaaacgg ccaggatctt gttttggaag acggaactga aatcaaggca
    38221 gcagactata tctcagcgcc acgtccaggt aagattatca ctattttagg agacactcga
    38281 aaaacgggtg ccagtgtgcg tctggctgtc aatgcagatg tcctagttca tgagtccact
    38341 tatggcaagg gtgatgaaaa aattgctcgt aaccatggtc actcaactaa tatgcaagct
    38401 gcacaagtag cggtagaagc aggtgccaaa cgcctcctac tcaaccatat cagtgcccgt
    38461 ttcctctcaa aagatattag caaactcaag aaagacgctg ccacaatttt tgaaaatgtc
    38521 catgtggtca aagacttgga agaagtggaa atctagcagt cacagaaagg ataagtatgc
    38581 ctactattct cattaccgga gctagcggtg gtctagctca agaaatggtc aaactcctgc
    38641 ccaatgacca actcatcttg cttggtagaa ataaggaaaa attagcccaa ctctacggaa
    38701 attattccca tgcagaattg attgaaattg atattaccga cgattcagcc ctagaagctc
    38761 tggtaactga tctttatctc cgctatggca agattgatgt cttgattaac aacgctggtt
    38821 acgggatttt tgagggattt gaccagattg ctgataaaga tattcaccag atgtttgagg
    38881 tcaatacctt tgccctgatg aatctgtctc gtcaccttgc ggctcgtatg aaggaaagca
    38941 gcaaagggca tatcatcaac atcgtcagca tggcaggtct aatagctact ggcaagtcta
    39001 gtctttactc agcgaccaag tttgcggcta ttggtttttc aaatgctctg cgactcgaac
    39061 ttatgcccta tggagtctat gtgacaacag tcaatccagg tccaatccga acaggatttt
    39121 tcgaccaagc cgacccagat ggaacttatc ttaaatcggt tgaccgcttc ctgctagaac
    39181 cagatgcagt ggctaaaaag attgtcaaga ttataggaaa aaacaaacga gaactcaatc
    39241 tcccgatttt gttgaaccta gctcataagt tttataccct ctttcctaag ctagctgata
    39301 agttggcagg ggaaactttt aattataagt aaaaagaacc aatgtgcagg ttgttgctag
    39361 cctacatatt ggttcttatc atttctcaac tatcaaactg aacttcttct aggagatagt
    39421 ctataaagcg ttcgcccatc ttagataggc tggttttctc atgctggata tagaccagct
    39481 cgatcgggtc atcaatatcc agtgggatag aaacgatatt gtctccgttt aggttgctgt
    39541 tcaaaatccc tgtcgcaatg gtataaccat ccaaaccaat caagagatta aagagggtgg
    39601 cacggtcact gaccacaatg gatttcttgt ggtgttcttg agaaagaatc tcttctgaaa
    39661 agtagaagga gttgtgcgtc ccttggtcat agctgaggta agggaaattc tccaaatcag
    39721 acagtttcac cttgtctttc tttgccagag ggttggtctt gctgacaaag atatgtggtt
    39781 gcgctgtgaa gagatggtgg gctagcaggt gattgtcatc cagcatcttg gttaaaacat
    39841 cacggttgta actgtttaag aagaggaccc cgacctcact gcggaagttc ttgacgtcgt
    39901 cgataatctc ccaagtccga gtttcacgaa ggaagagttc gtatttctcc atatcgcttt
    39961 tcttgagcaa agagacaaag gcattgacca caaaggcata gtgttgagac gaaacgctaa
    40021 atagttcgcg gtgggcgaca ggatttttat agcgttcctc cagaagctgg gtctgctcga
    40081 caacctgacg ggcataagag agaaactcca tcccatcacg ggtcaaggtg attcccttgg
    40141 gattgcggat aaagatctca atgcccattt cattttccaa atctcgcact gcattggaga
    40201 gacttggctg agtgataaag agttgcttgg ctgcctcatt catggagcca gtttcgacga
    40261 ttttgataat ataatgtaat tgttgaattc tcatgttttt attgtaccat acttgcctta
    40321 gaatcgctaa agaagatagg aaaataagga gcattgtgtg agtctcttct tgaaaaaaac
    40381 acaaaatttt gctagaatga gtcttatgaa aacattctat gatgtgcagc aattcctcaa
    40441 acgatttggt attattgttt acatgggaaa acgcttatat gatattgaac tgatgaagtt
    40501 ggaactatct cggatttacg atgcagggtt gatggataaa ttagactatc tagaggcaga
    40561 agcagttctt cgcagagagc acaaggtaga attggattat attgagaaaa atggagaaaa
    40621 gaactaatgg ttacttggat tttgtgggca cttatactag caatgttggc gtggatgggc
    40681 tttaactatc ttcgtattcg ccgtgcggct aaaattgtgg acaatgagga gtttgaagcc
    40741 ttgattcgta cgggtcaatt gattgatttg cgcgacccag cagaattcca cagaaaacat
    40801 atccttggtg cacgcaatat tccttcaagt cagttgaaaa ctagtcttgc agcccttcgt
    40861 aaagataaac ctgtccttct ctacgagaac caacgtgcgc aacgagttac aaatgcagct
    40921 ctttacttga aaaaacaagg tttttctgag atttatatcc tttcttatgg cttggattct
    40981 tggaaaggga aagtgaagac tagctaacct tatttccaaa ttagtcgaat gataggatga
    41041 agctattgtt ttatagtata attaatttgt ataaaattta aggagttctt agaacataaa
    41101 agaaaagaag aaaaatccac tttttgtggg tatcttgtcg attattcttg gtttgttatt
    41161 tccaattgta ggcctgattt tgggtatcat aggattggtc ttggctattt cataccaaaa
    41221 agagtcataa ttagactata aaatagaaaa gattcttaat atcttaggaa ttgtgatttc
    41281 tgtagttaac tggattgtag caatcgcctt aatttttcgg taaataaaag aaaaaagctt
    41341 tagttttaaa gctttttttg ttttatcgac tcatctctaa atagttttta atgatttgta
    41401 actctttttg gttcaaaggg cggtagttac tttctgctaa atctgggttc aatgtgaagt
    41461 ccccaaattg gattctcttg aggatagtca ccttaacacc aaccgagagg aacattttct
    41521 tgatttgatg aaattttcct tctgaaatgg tgatagaggc ttgactgatg gagggacttg
    41581 cagatagaat ctctagtttt gcgggtttac agacagtacc atctaaaaag acaattccct
    41641 tttgaaaggc ttggatatgg tcaggtgtta gaagtccatt aacctcaact tgataagtct
    41701 tatcgacatg atattgggga tggaggagtt ggaagcccaa gggaccgtta tcggtcaaga
    41761 ggaggagtcc cgttgtatct cggtccagtc ggccaacggc atagagcttg tcagactgga
    41821 tgtcagatgg aagcaggtcc atgacggtcg gaagttcctt gtctttgttg gctgtaacga
    41881 caccagcagg tttatgaagc ataagatagg tgtgttcata gccttgaatg attcgatcct
    41941 gaaaaaggag ttcttgtagt cctgtatcga tattttgagc tagggagcgg gctgggcaac
    42001 catcgactag aattttccct ctgaggagtg cttgcttcat ggcctttcgg ctgatttttt
    42061 cttgggctaa taaattatct aaacgcatac tgtgcctatc ttatcataag tcgcttgctt
    42121 tgaaaaggct tgacgtgaaa agaaaagcgg agtgaaaaca tttacacttg cttgagttat
    42181 gttatttatt tgaaattatg gtataatcgt tcagttagaa aataaatttt gaatattata
    42241 gaggaaatca tgacaaaatt aagagaagat atccgtaaca ttgcgattat cgcccacgtt
    42301 gaccacggta aaacaaccct ggttgacgaa ttattgaaac aatcagaaac gcttgatgca
    42361 cgtactgaat tggcagagcg tgctatggac tcaaacgata tcgaaaaaga gcgtggaatc
    42421 accatccttg ctaaaaatac tgccgttgct tacaacggaa ctcgtatcaa cattatggac
    42481 acaccaggac acgcggactt cggtggagaa gttgagcgta tcatgaaaat ggttgacggt
    42541 gttgtcttgg tcgtagatgc ctatgaagga accatgccac aaactcgttt cgtattgaaa
    42601 aaagccttgg aacaagacct tgtcccaatc gtggttgtta acaaaatcga taagccatca
    42661 gctcgtccag cagaagtagt ggatgaagtc ttggaacttt tcatcgagct tggtgcagat
    42721 gacgaccagc ttgatttccc agtggtttat gcttcagcga tcaacggaac ttcttcattg
    42781 tcagatgatc cagctgacca agaagcgact atggcaccaa tctttgacac gattatcgac
    42841 catatcccag ctccagtaga taactcagat gagcctttgc agttccaagt gtcacttttg
    42901 gactacaatg acttcgttgg acgtatcggt atcggtcgtg tcttccgtgg tacagttaag
    42961 gttggggacc aagttaccct ttctaaactt gacggtacaa ctaaaaactt ccgtgttaca
    43021 aaactcttcg gtttctttgg tttggaacgt cgtgaaatcc aagaagccaa agcgggtgac
    43081 ttgattgccg tttcaggtat ggaagacatc tttgtcggtg aaaccatcac tccgacagat
    43141 gcagtagaag ctcttccaat cctacacatc gatgagccaa ctcttcaaat gactttcttg
    43201 gtcaacaact caccatttgc tggtaaagaa ggtaaatggg taacttctcg taaggtggaa
    43261 gaacgcttgc aggcagaatt gcaaacagac gtttcccttc gtgttgaccc aactgattca
    43321 ccagataaat ggactgtttc aggacgtgga gaattgcact tgtcaatcct tatcgaaaca
    43381 atgcgtcgtg agggctatga acttcaagta tctcgtccag aagttatcgt aaaagaaatc
    43441 gacggtgtta aatgtgaacc atttgaacgt gtacaaatcg acactccaga agaataccaa
    43501 gggtctgtta tccaaagcct ttctgaacgt aaaggtgaaa tgttggatat gatttcaact
    43561 ggtaatggtc aaactcgttt ggtcttcctt gttccagcgc gtggtttgat tggatactca
    43621 actgagttct tgtcaatgac tcgtggttac ggtatcatga accatacctt cgaccaatac
    43681 ttgccattga ttccagggga aattggtgga cgtcaccgtg gtgcccttgt ttctatcgat
    43741 gctggtaagg ctacaactta ctcaatcatg tctatcgaag aacgtggtac gatctttgtc
    43801 aacccaggta ctgaggttta tgaaggaatg atcatcggtg aaaactctcg tgaaaatgac
    43861 ttgacagtta acatcacgaa ggcaaaacaa atgaccaacg ttcgttcagc tactaaggac
    43921 caaacagctg ttatcaagac gcctcgtatc ttgacacttg aagagtctct tgagttcttg
    43981 aacgacgatg agtacatgga agtaacgcct gagtctatcc gtttgcgtaa acaaatcctt
    44041 aacaaagcag agcgtgagaa agctaacaag aagaaaaaat cagctgaata agagctagaa
    44101 agagagaaag atggtctatt tagtcctagg aattttactg ctcctactct atgtatttgc
    44161 gacaccagaa agcattaaag ggacagtcaa tatcgtcgct atggtatgta ttttagtggc
    44221 actcttgatt ttattggttc tatcttttct gaaaattttt caattaccaa cagaaatatt
    44281 cctagcaata gccatgttgc tcctagctta ctttagtgtt agagacatta cactcatgcc
    44341 agtcaaaaaa agtaaaagaa gataaaaaag agagttttgg ctctcttttt tgctaggaat
    44401 tttacaagat tgcaatttat ttttgagtta tcacc
//
LOCUS       DAYYQN010000008        39702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYYQN010000008 DAYYQN010000000
VERSION     DAYYQN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.008"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            527..2680
                     /gene="comA"
                     /locus_tag="ACHBRQ_000425"
     CDS             527..2680
                     /gene="comA"
                     /locus_tag="ACHBRQ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HFM9592151.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2693..4042
                     /gene="comB"
                     /locus_tag="ACHBRQ_000426"
     CDS             2693..4042
                     /gene="comB"
                     /locus_tag="ACHBRQ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HFM9592152.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQGGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4212..4919
                     /gene="purC"
                     /locus_tag="ACHBRQ_000427"
     CDS             4212..4919
                     /gene="purC"
                     /locus_tag="ACHBRQ_000427"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9592153.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4976..8701
                     /locus_tag="ACHBRQ_000428"
     CDS             4976..8701
                     /locus_tag="ACHBRQ_000428"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFM9592154.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLSIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGVIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLSELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLSYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8794..10236
                     /gene="purF"
                     /locus_tag="ACHBRQ_000429"
     CDS             8794..10236
                     /gene="purF"
                     /locus_tag="ACHBRQ_000429"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9592155.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FYDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10273..11295
                     /gene="purM"
                     /locus_tag="ACHBRQ_000430"
     CDS             10273..11295
                     /gene="purM"
                     /locus_tag="ACHBRQ_000430"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9592156.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11292..11837
                     /gene="purN"
                     /locus_tag="ACHBRQ_000431"
     CDS             11292..11837
                     /gene="purN"
                     /locus_tag="ACHBRQ_000431"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9592157.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11921..12430
                     /locus_tag="ACHBRQ_000432"
     CDS             11921..12430
                     /locus_tag="ACHBRQ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9592158.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12455..14002
                     /gene="purH"
                     /locus_tag="ACHBRQ_000433"
     CDS             12455..14002
                     /gene="purH"
                     /locus_tag="ACHBRQ_000433"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9592159.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     NDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            14124..15386
                     /gene="purD"
                     /locus_tag="ACHBRQ_000434"
     CDS             14124..15386
                     /gene="purD"
                     /locus_tag="ACHBRQ_000434"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9592160.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNIFISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTVATVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15643..15759
                     /locus_tag="ACHBRQ_000435"
     CDS             15643..15759
                     /locus_tag="ACHBRQ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HFM9592161.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            15789..16277
                     /gene="purE"
                     /locus_tag="ACHBRQ_000436"
     CDS             15789..16277
                     /gene="purE"
                     /locus_tag="ACHBRQ_000436"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9592162.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16264..17355
                     /gene="purK"
                     /locus_tag="ACHBRQ_000437"
     CDS             16264..17355
                     /gene="purK"
                     /locus_tag="ACHBRQ_000437"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9592163.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAKVTVAPYKVVTSSQDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVQA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAQLPAIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17365..17592
                     /locus_tag="ACHBRQ_000438"
     CDS             17365..17592
                     /locus_tag="ACHBRQ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HFM9592164.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17655..18953
                     /gene="purB"
                     /locus_tag="ACHBRQ_000439"
     CDS             17655..18953
                     /gene="purB"
                     /locus_tag="ACHBRQ_000439"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9592165.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19008..22934)
                     /gene="strH"
                     /locus_tag="ACHBRQ_000440"
     CDS             complement(19008..22934)
                     /gene="strH"
                     /locus_tag="ACHBRQ_000440"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HFM9592166.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDKAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23248..23964)
                     /locus_tag="ACHBRQ_000441"
     CDS             complement(23248..23964)
                     /locus_tag="ACHBRQ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9592167.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24312..26099
                     /locus_tag="ACHBRQ_000442"
     CDS             24312..26099
                     /locus_tag="ACHBRQ_000442"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFM9592168.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26096..26572
                     /locus_tag="ACHBRQ_000443"
     CDS             26096..26572
                     /locus_tag="ACHBRQ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9592169.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26600..27505
                     /locus_tag="ACHBRQ_000444"
     CDS             26600..27505
                     /locus_tag="ACHBRQ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9592170.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27492..28307
                     /locus_tag="ACHBRQ_000445"
     CDS             27492..28307
                     /locus_tag="ACHBRQ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9592171.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28314..28718
                     /locus_tag="ACHBRQ_000446"
     CDS             28314..28718
                     /locus_tag="ACHBRQ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9592172.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29017..30183
                     /locus_tag="ACHBRQ_000447"
     CDS             29017..30183
                     /locus_tag="ACHBRQ_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9592173.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30299..31336
                     /locus_tag="ACHBRQ_000448"
     CDS             30299..31336
                     /locus_tag="ACHBRQ_000448"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9592174.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31543..32397
                     /locus_tag="ACHBRQ_000449"
     CDS             31543..32397
                     /locus_tag="ACHBRQ_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9592175.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGIGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     RGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            32547..32783
                     /locus_tag="ACHBRQ_000450"
     CDS             32547..32783
                     /locus_tag="ACHBRQ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9592176.1"
                     /translation="MTNSKYITRLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAI"
     gene            complement(32912..33208)
                     /locus_tag="ACHBRQ_000451"
     CDS             complement(32912..33208)
                     /locus_tag="ACHBRQ_000451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592177.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGAVHFNT"
     gene            complement(33262..33558)
                     /locus_tag="ACHBRQ_000452"
     CDS             complement(33262..33558)
                     /locus_tag="ACHBRQ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9592178.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNTWPGQVE"
     gene            complement(33548..33730)
                     /locus_tag="ACHBRQ_000453"
                     /pseudo
     CDS             complement(33548..33730)
                     /locus_tag="ACHBRQ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            33779..34543
                     /locus_tag="ACHBRQ_000454"
     CDS             33779..34543
                     /locus_tag="ACHBRQ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HFM9592179.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            35156..36535
                     /locus_tag="ACHBRQ_000455"
     CDS             35156..36535
                     /locus_tag="ACHBRQ_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9592180.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36549..37214
                     /locus_tag="ACHBRQ_000456"
     CDS             36549..37214
                     /locus_tag="ACHBRQ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9592181.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            37524..38062
                     /locus_tag="ACHBRQ_000457"
                     /pseudo
     CDS             37524..38062
                     /locus_tag="ACHBRQ_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            38138..38539
                     /locus_tag="ACHBRQ_000458"
     CDS             38138..38539
                     /locus_tag="ACHBRQ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFM9592182.1"
                     /translation="MPVDFYRYLIRREDQSVNEQVMIKCIDQQLKVNRLLVDQLDLSQ
                     VSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAIRKT
                     MLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            38655..>39702
                     /gene="pavB"
                     /locus_tag="ACHBRQ_000459"
     CDS             38655..>39702
                     /gene="pavB"
                     /locus_tag="ACHBRQ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HFM9592183.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLT"
BASE COUNT        10920 a         7717 c         9274 g        11791 t
ORIGIN      
        1 gatataaagt ttgcctattt cagcgtattg acagtattac taattgggat aacaaattat
       61 gcgagattta aaaatttaag attgacagta gtgttttctg taccattttt atttaatgct
      121 ataatgtcta ttataaatag tttttagaaa tactaaaatt tttttcttta cttaataatg
      181 atatgaaatc atttcggttt tgttaacttg taattcttat attttctagg tagaacgttt
      241 gttctgccta tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa
      301 ggaagtccag tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac
      361 tcttcgaaaa tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact
      421 tcgtcagtcc tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta
      481 gtttgctctt tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa
      541 acgtcactat cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt
      601 ttttggctac tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac
      661 catggatggg acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac
      721 gcgagccatt aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt
      781 tgcccatgtg cttaaggaag ggaaattgct ccactactat gtggtgactg ggcaggataa
      841 ggatagcatt catattgccg acccagatcc tggggtgaaa ttgactaaac tgccacgtga
      901 gcgttttgcg gaagaatgga caggagtgac tctttttatg gcacctagtc cagactacaa
      961 gcctcataag gaacaaaaaa atggtctgtt ctcttttatc cctatattag tgaagcagcg
     1021 tggcttgatt gccaatatcg ttttggcaac actcttggta accgtgatta acattgtggg
     1081 ttcttattat ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact
     1141 agggattatt tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc
     1201 tcaggagtat ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta
     1261 tatcaagcat gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat
     1321 cgtgtctcgt tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc
     1381 gattttccta gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac
     1441 caatctcttt ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt
     1501 tatgaagccg tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc
     1561 ttctatcatt gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca
     1621 gcgttaccaa aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag
     1681 tcgagcagag agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg
     1741 cattctctgg atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat
     1801 tacctataat accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca
     1861 aaccaagctt cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagc
     1921 ttctgagttt gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagatatgac
     1981 cttcaagcag gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt
     2041 aaccgttccc caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac
     2101 tttggccaag atgatggtta atttttacga cccaagtcaa ggggagatta gtctgggtag
     2161 tgtcaatctc aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca
     2221 acagccctat gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg
     2281 gacgacacag gaagatatct tacgggcggt cgaattggca gagattcgag aggatatcga
     2341 gcgcatgcca ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg
     2401 tcaacgtcag agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt
     2461 ggatgaggcg actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat
     2521 ggctttggac aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga
     2581 gaaggtggtt gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct
     2641 tgcacaggga ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc
     2701 agaattttta gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt
     2761 gattgtaccc atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa
     2821 ggagatgagt ttgtccacta gagctactgt cgaacctagt cgtatccttg caaatatcca
     2881 gtcaactagc aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa
     2941 gggggatctt ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc
     3001 cagtcagttg gacatgctaa aggatcaaaa aaagcaattg gagtatctgc aaaagagcct
     3061 gcaaggaggg gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg
     3121 cgactacatc agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat
     3181 cgcgtcccag aatgcagcag ctagccaaac ccaagccgaa atcggcaacc tcatcagtca
     3241 aacagaggct aaaattcgcg attaccagac agctaagtca gctattgaaa caggtgcttc
     3301 cttggccggt cagaatctag cctactctct ttaccagtcc tacaagtctc agggcgaaga
     3361 aaatccccaa actaaggttc aggcagttgc acaggttgaa gcacagattt ctcagttaga
     3421 atctagtctt gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc
     3481 gtcagggtta agcagtcaat tggaatccct taaatcccaa cacttggcaa aggttggtca
     3541 ggaattgacc cttctagccc agaaaatttt ggaggcagag tcaggtaaga aggtacaggg
     3601 aaatctttta gacaagggga aagttacggc gagtgaggat ggggtgcttc atcttaatcc
     3661 tgagaccagt gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc
     3721 tttggaaaga gaagggaaag ccaaactcac agcttatcta agttcaaaag atgtagcaag
     3781 aatcaaggtc ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt
     3841 cctagattct actattacaa gtattgatgc gacagctact aagactgaga aagggaattt
     3901 ctttaaaatc gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtatgg
     3961 ggtggaaggc cgtttacaga tgattacggg caagaaaagt tatctacgtt attatttgga
     4021 tcaatttttg aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg
     4081 agaaagattc ttcttgcagt tttttcttta caatttttga aaagcatctg ctatttattc
     4141 ggttaaattc ttgtgttttt tggttttttg tggtaaaatg tgctcaagta atacgaaagg
     4201 cgaactttaa aatgtcaaaa caattgatct attcgggaaa agctaaagat atctatacaa
     4261 ctgaggatga aaatcttatt atttcaactt acaaggacca ggcgactgct ttcaacggtg
     4321 tcaagaagga gcagattgca ggtaagggag tcttgaataa tcagatctca tcttttattt
     4381 ttgagaaatt aaatgcggct ggtgtggcga ctcactttgt ggagaaactt tcagacacgg
     4441 aacaactcaa taaaaaggtt aagattattc ctttggaagt cgtgctccgc aactatactg
     4501 ctggttcctt ttcaaaacgt tttggtgtgg atgagggaat cgccttggag actccgattg
     4561 tcgaatttta ctataaaaat gatgatttgg atgatccatt tatcaatgat gagcatgtga
     4621 aattcctaca gattgcggat gaccagcaga ttgcctactt gaaggaagaa acgcgtcgta
     4681 tcaatgaact attgaaagtc tggtttgctg agattgggct taaattgatt gactttaagc
     4741 tagagttcgg ttttgacaag gatggcaaga ttatcttggc agacgaattt tcaccagata
     4801 actgccgctt gtgggatgct gatggcaacc acatggataa ggatgttttc cgtagaggat
     4861 tgggagaact aaccgacgtt tacgaaattg tctgggaaaa gttgcagggt ttaaaataac
     4921 aacctcaagg ctgtttggga atattgcaag agctgaaata aaggaataag aattgatgga
     4981 taaacgtatt tttgttgaaa aaaaggctga ttttcaggtc aagtcagaga gtttggttag
     5041 agagctccag cacaacttgg gactgtcaag cttgaaaagt attcgtattg tgcaagtata
     5101 tgatgtattt gacttggttg aggacttgtt tgcacctgca gagaagcaca ttttctctga
     5161 gcaggtaacc gaccatgttt tagatgaagt atctgtgcag gcggatcttg ctaactatgc
     5221 tttctttgcc attgaaagtc taccagggca gtttgaccag cgtgcagctt cgtcacagga
     5281 agccttgctt ttgttgggaa gttcgagtga cgtgacagtc aacacagccc aactttactt
     5341 ggtgaataaa gatattgatg cgactgagtt ggaagctgtc aaaaactacc tgctcaatcc
     5401 agttgattct cgtttcaagg atatcacgat agggattgcc aagcaggagt tttcagagtc
     5461 agacaagacc attccaaaat tgcctttctt tgaaagctat acagcagaag actttgctcg
     5521 ctacaaggcc gaacaaggga tggccatgga agtggatgat ttgctctcta tccaagacta
     5581 ctttaagtca atcgggcgcg tgccaactga gactgaactc aaggttttgg acacttactg
     5641 gtctgaccac tgccgtcata cgacttttga gacagagttg aaacacatcg acttttcagc
     5701 ttctaaattt caaaagcaat tgcagtcaac ctatgacaag tatattgcca tgcgcgagga
     5761 attaggtcgg tctgaaaaac cacaaacctt gatggatatg gcgactattt tcggtcgtta
     5821 tgagcgtgct aatggacgat tggatgatat ggaagtctct gacgaaatca atgcctgctc
     5881 agttgaaatt gaagtggacg ttgatggtgt caaggaacct tggctcctca tgtttaaaaa
     5941 cgaaacccac aaccatccaa cagaaattga gccatttggt ggagcggcta cctgtatcgg
     6001 tggagttatt cgtgatccgt tgtcaggccg ttcctatgtt taccaagcca tgcgtatttc
     6061 aggtgctggt gatattacag caccgatttc ggaaactcgt gctgggaaat tgccacaaca
     6121 agtcatttct aaaacagcaa ctcatggtta ttcttcatat ggtaaccaga ttgggcttgc
     6181 cacaacctac gttcgtgaat acttccaccc aggctttgta gctaaacgta tggaacttgg
     6241 tgccgttgtt ggtgcggctc ccaagggcaa tgttgtccgt gaaaaacctg aagcaggtga
     6301 tgtgatcatc cttctcggag gtaaaacagg tcgtgatggt gtcggtggtg cgacgggctc
     6361 ttctaaggtt caaacagttg agtctgtaga gactgctggt gctgaggttc aaaaaggaaa
     6421 tgccatcgaa gaacgcaaga ttcagcgcct cttccgtaat ggcaatgtca ctcgtctgat
     6481 caagaagtcc aatgactttg gggcaggcgg tgtctgtgtg gctatcggtg aattggcaga
     6541 cggtcttgaa atcgacctca acaaggtgcc tcttaaatac cagggcttga atggtacaga
     6601 aattgccatc tctgaatcac aagaacggat ggcggtcgtg gttcgtcctg aagatgtgga
     6661 taccttcgtt gccgaatgta acaaagaaaa tattgatgct gttgtggtgg cgacagtaac
     6721 tgaaaaacca aatcttgtca tgcactggaa tggtgagaca atcgttgact tggagcgtcg
     6781 tttccttgac accaatggtg tgcgcgtggt tgtcgatgcc aaagttgtgg acaaggatgt
     6841 caaactccca gaagagcgtc aaacatctgc tgaaacactg gaatcagata cccttacggt
     6901 tctatctgac ctcaaccatg caagtcaaaa aggattacag actatctttg actgctctgt
     6961 tggacgctca acggttaatc acccacttgg tggccgttac caactcacac caactgaggc
     7021 atctgtgcag aaattgccag ttcaacacgg tgtgactcat actgcgtcgg tcattgctca
     7081 aggtttcaac ccatatgtag ctgaatggtc tccataccac ggtgctgctt atgcggttat
     7141 cgaagcaacc gctcgtttgg ttgctgctgg tgccaactgg tccaaggctc gtttctctta
     7201 ccaagagtat ttcgagcgta tggataaaca agcagagcgt ttcggtcagc cagtagctgc
     7261 tcttctaggt tctattgaag cacaaattca gcttggcttg ccatctatcg gtggtaagga
     7321 ctccatgtct ggtacctttg aagaattgac cgttccgcca accttggttg cctttggggt
     7381 gacgacggca gatagccgta aggtgctctc tccagaattt aaagctgttg gggaaaatat
     7441 ctactacatc ccaggtcaag ccctctctgc agagattgat tttgacttga ttaagaaaaa
     7501 ttttgctcag tttgaagcca tccaagctga ccataaagtg acatctgcat cagctgtcaa
     7561 atacggtggt gtagttgaaa gtttggctct tgctaccttt ggaaactata ttggtgcaga
     7621 ggtgaccttg tctgaactta aaacagcttt gacagctcaa ttaggcggct ttgtcttcac
     7681 atctcctgaa gaaattgctg gagtagagaa gattggacaa acgaaagcag actttacact
     7741 gactgtcaac ggtgtgaagc tagatggaca caagcttgac agtgcatttc aagggacatt
     7801 ggaagaagtt tacccaacag aatttaccca agcaaaagaa ctagaagaag taccagctgt
     7861 ggcatcagat gttgtgatta aagccaaaga aaaggttgaa aagcctgtgg tttacatccc
     7921 agtctttcca ggaaccaact cagaatatga ttcagctaag gccttcgaaa aagaaggtgc
     7981 agaggtcaat ttggtgccat tcgtgacctt gaatgaagaa gctattgtca agtcagttga
     8041 aactatggtt gacaatatcg acaagactaa tattctcttc tttgccggtg gattctcggc
     8101 tgcggatgaa ccagatggtt cagctaagtt tatcgtcaat atcctgctta atgaaaaagt
     8161 gcgtgtggct attgatagct ttatcgcccg tggtggtttg attatcggta tttgtaatgg
     8221 attccaagcc ttagtcaaat cgggtctcct atcatacgga aactttgaag atgctaacag
     8281 tactagtcca accctcttct acaatgatgc caaccaacac gtggccaaga tggtggaaac
     8341 tcgcattgcc aataccaact caccatggtt ggctggtgtg caagtgggcg atatccacgc
     8401 tattcctgtt tcgcacggtg aagggaagtt tgtcgtgacg gctgaggaat ttgcagagct
     8461 ccgtgacaat ggacaaattt tcagccaata cgttgacttt aacggtaaac caagtatgga
     8521 ttctaagtac aatccgaatg gttctgtcca tgccatcgaa ggaattacca gcaagaatgg
     8581 tcaaatcatc ggtaagatgg gccactcaga acgttatgag gatggtcttt tccaaaatat
     8641 cccaggcaat aaagaccaac acctgttcgc atcagcggtt aaacatttca ctggaaaata
     8701 agacttacag attttctaat agatagtatc agtaatgtaa aagtcatgta aatctagctc
     8761 ttgatgatta caaatgaaaa ttaggtataa aaaatgacat acgaagtaaa atctcttaat
     8821 gaagaatgtg gtgttttcgg tatttgggga catccagatg ctgctaagtt gacctatttt
     8881 ggactccaca gtcttcaaca ccgtggtcag gagggggcag gaatcctctc caatgatcaa
     8941 ggacaactga agcgccatcg tgacatgggg cttttatcag aagttttcag aaatccagct
     9001 aatttggata aattgacagg agctggtgcg attgggcatg tgcgttatgc gactgctggc
     9061 gaagcttctg tagataacat ccagcccttc ctcttccgtt tttacgatat gcagtttggc
     9121 ttggctcata atggaaatct gaccaatgca gcctctctca agaaagaact ggaacaaaga
     9181 ggagcaattt tcagcgcgac ttcggactct gaaatcttgg ctcacctcat tcgtcgcagt
     9241 cataatccta gcctgatggg caaaatcaag gaagcgctca gccttgtcaa aggtggtttt
     9301 gcctatatct tgctgtttga ggacaagttg attgcggctc ttgacccaaa tggattccga
     9361 ccgctttcga ttggtaaaat ggctaatgga gcagtagtcg tttcctctga aacctgtgct
     9421 tttgaggtca ttggtgccga gtggattcgt gatttgaagc caggtgagat tgtgatcatt
     9481 gatgacgagg gcattcagta tgatagctat acagatgata cccagttggc gatttgttct
     9541 atggagtata tctactttgc tcgccctgat tctaatatcc acggtgtcaa tgtccatacg
     9601 gcacgtaaga gaatgggagc acaattggcg cgagaattta agcatgaggc agatattgta
     9661 gttggtgtgc ccaattcttc cctaagcgcg gctatgggat ttgcggaaga atcaggctta
     9721 ccaaatgaaa tgggtctgat caaaaaccaa tacacccagc gaacttttat ccaaccgact
     9781 caagaattgc gggagcaagg ggtgcggatg aaactgtctg ctgtttcggg tgttgtcaaa
     9841 ggcaaacgtg tggtcatggt ggatgattcc attgtacgtg gaacaacctc tcgtcgtatc
     9901 gttcagctct tgaaagaagc gggtgcgact gaggttcacg ttgccattgg aagtcctgca
     9961 ctagcgtatc catgtttcta cgggattgat atccagaccc gtcaggagct gattgcagcc
    10021 aatcatacgg tcgaagaaac tcgccaaatc attggtgcgg acagtctgac ttatctttca
    10081 attgatggct tgattgagtc gattggtatc gaaacagatg cgccgaacgg tggtctctgt
    10141 gtcgcttact ttgacggtga ctacccaacg cctctttatg actacgaaga agactatcgt
    10201 agaagtttgg aagaaaagac cagtttttac aagtaggcga cagattctcc attaaagaaa
    10261 aggaaaaaac aaatgacaaa taaaaatgca tatgcccagt cgggtgtgga tgttgaagcg
    10321 ggttatgaag ttgttgaacg gattaaaaag cacgtggccc gtacggagcg tgcaggtgtc
    10381 atgggagctc ttggtggctt tggtggtatg tttgaccttt ccaagactgg ggttaaagaa
    10441 cccgtcttga tttcagggac tgacggtgtc ggaaccaagc tcatgttggc tatcaagtac
    10501 gacaagcacg ataccatcgg gcaggactgt gtggccatgt gtgtcaacga catcattgct
    10561 gcaggtgcgg aacccctcta ttttctcgac tacgtagcga cagggaagaa tgaaccagct
    10621 aagctagaac aagtggttgc tggtgtggca gaaggttgtg tgcaggctgg tgctgccctc
    10681 atcggtgggg aaacggctga aatgccgggc atgtacggcg aagacgacta tgacttggct
    10741 ggttttgcgg tcggtgtggc tgaaaaatct caaatcattg acggttcaaa ggtggtagag
    10801 ggagatgttc ttctcggact tgcttcaagt gggattcact caaatggtta ctctttggtt
    10861 cgtcgtgtct ttgcggatta cacaggtgag gaagtcctac cagaattgga aggcaagaaa
    10921 cttaaggaag ttctacttga gccgactcgt atctatgtca aggctgtctt gccgctcatc
    10981 aaagaagagt tggtcaacgg cattgcccac atcacaggtg gtggctttat cgaaaatgtc
    11041 cctcgtatgt ttgcagatga cctagctgct gaaattgatg aaagcaaagt tccagtgctt
    11101 ccaattttca aagcccttga aaaatatggt cagattaaac acgaagaaat gtttgaaatc
    11161 ttcaatatgg gtgtgggact tatgttggcg gtcagccctg aaaatgtaga gcgtgtaaaa
    11221 gaattgttgg atgaagcagt ctatgaaatt ggtcgcatcg tcaagaaaga aaacgaaagt
    11281 gtcattatca aatgaaaaaa atagcggttt ttgcctctgg taatggctca aattttcagg
    11341 tgattgccga agaatttcca gtggagtttg tcttttcaga ccatcgtgat gcctatgtgc
    11401 ttgagcgtgc aaagcagctc ggcgttctgt cctatgcttt tgaactcaag gagtttgaga
    11461 gcaagacaga ctacgaagca gcccttgtcg aactcttgga agaacaccag attgacttgg
    11521 tttgcctagc aggctacatg aaaatcgttg gaccaacctt attgtcggct tatgaaggtc
    11581 ggattgtcaa cattcatcca gcctacttgc cagaatttcc aggagctcat gggattgagg
    11641 atgcttggaa tgctggcgtg ggtcagtctg gtgtgaccat tcactgggtg gattcgggtg
    11701 tggatacagg ccaggtcatc aaacaggttc gtgtgccacg actagctgat gataccattg
    11761 acagatttga agctcgcatc catgaagcag agtacaggtt gtatccggaa gtagtgaagg
    11821 ctctatttac agattgactt tttgatgatt catatgatat ctttgatttt aaattggagt
    11881 cagtgtttgt tgaagacggc ttcaaacgga ggtatttgta atgttagaat ctaaaaaaac
    11941 aactcgatat gtattttatg tctatctgat gttattaact tggggaatct tatttaagtt
    12001 tgaaacaaat cctgaattta tagcattttt cttagctcca aggtatatca attggattcc
    12061 attttcagaa ccactaatag tcgatggaaa aattgttttt gctgaaatgt tatttaatct
    12121 gatttccttt attccattag gtgtttgttt ccctttgata aaaactaatt tatctagttt
    12181 aagaatagtc gggacaggtt tcttgattag tttattgttt gagtgcttac agtatatttt
    12241 agcaataggt ataacagata taacggattt gactttaaat acgctaggtg tctgtgtagg
    12301 cttactgatt tatcaaattt ttataagagt gttcaaatca cagactagaa aatggatcaa
    12361 tatcttaggt atgcttagcc ttggttttgc ttatcttgtt ttactgttac tgcatttaat
    12421 tggtgtttaa ctaatgatta aaaaggagaa tataatgact aaacgcgtct taatcagcgt
    12481 ctcagacaaa gcgggcattg ttgaatttgc ccaagaactc aaaaaacttg gttgggagat
    12541 tatctcaaca ggtggaacta aggttgccct tgataatgct ggggtggata ccattgctat
    12601 cgatgatgtg actggtttcc cagagatgat ggatggccgt gttaagactc ttcatccaaa
    12661 tatccacggg ggtcttctcg ctcgtcgtga tttggatagc cacttggaag cagccaatga
    12721 gaaccaaatt gagcttattg accttgtagt ggtcaacctt tacccattta aggaaactat
    12781 ccttaaacca gatgtgactt atgctgatgc agttgaaaat atcgatattg gtgggccgtc
    12841 tatgcttcgt tcagcagcga aaaatcatgc cagtgttaca gttgtggtag atcctgctga
    12901 ctacgctgtg gttttggacg aattggcagc aaacggcgaa acctcttatg aaactcgcca
    12961 acgtttagca gccaaagtat ttcgtcacac agcagcttat gacgccttga ttgcagaata
    13021 cttcacagct caagtgggtg aaagcaagcc tgaaaaactc actttgactt atgacctcaa
    13081 gcaaccaatg cgttacggtg agaatcctca acaagacgcg gacttttacc agaaagcttt
    13141 gcctacagat tactctattg cttcagccaa acagctcaac gggaaagaat tgtcatttaa
    13201 taatatccgt gatgcagatg ctgctatccg tatcatccgt gacttcaaag atagtccaac
    13261 cgttgtggct ctcaaacaca tgaatccatg tggaattggt caagctgatg acatcgagac
    13321 tgcttgggac tacgcttatg agtctgaccc agtatctatc tttggtggga ttgtcgtcct
    13381 caaccgtgag gtggatgctg cgacagctga gaagatgcac ggcgttttcc tcgaaatcat
    13441 cattgcacca agctatacgg atgaagcgct agctattttg atcaataaaa agaaaaactt
    13501 gcgtatcctt gccttgccat ttaatgctca agaggctagc gaagtggaag cagaatacac
    13561 aggtgtagtc ggtggacttc tcgtgcaaaa tcaagacgtg gtcaaggaaa gcccagctga
    13621 ctggcaagtg gtgaccaaac gtcagccaac tgagacagaa gcgactgctc ttgagttcgc
    13681 ttggaaggct atcaagtacg tcaaatcaaa tggtattatc gtgaccaacg accacatgac
    13741 acttggtgtt ggtccaggtc aaaccaaccg tgtggcttct gttcgccttg ccattgacca
    13801 agccaatgat cgtctggacg gggcggtcct tgcttcagat gccttcttcc catttgcgga
    13861 taacgtggaa gaaatcgcca aagcaggaat taaggccatc atccagcccg gtggctctgt
    13921 ccgtgaccaa gaatccatcg aagcagcgaa taaatacggc ttgactatgg tctttacagg
    13981 tgtgagacat tttagacatt aagaagataa aagggaagaa aacagtttct ttcctttttt
    14041 ggcttaaaat gctaactgaa acaagattaa aacgaacttt tttgatataa tgttggtaaa
    14101 taattcgcaa aagaggttga ggaatgaaac tgcttgttgt cggttctggt ggtcgtgagc
    14161 atgcgattgc taaaaagtta cttgaatcaa aagacgtgga aaaagtcttt gtagctcctg
    14221 ggaatgatgg gatgactctg gatggtttgg aattggtaaa tatctttatt tccgaacatt
    14281 ctaaattgat tgacttcgca aagaccaatg atgttgcttg gacctttatc ggtccagatg
    14341 atgcccttgc tgctggtatc gtggatgatt ttaaccaagc tgggcttaag gcctttggtc
    14401 cgactagggc tgcagcggag ctggagtggt ccaaggattt tgccaaggaa atcatggtca
    14461 aatatggtgt ttcgacagca gcctatggca cattttcaga tttcgaggaa gcaaaggcct
    14521 atatcgaaaa gcatggtgcg cctatcgtag tcaaggcgga tggcttggcg cttgggaagg
    14581 gtgtcgtcgt tgctgagacg gttgagcaag cggtcgaagc cgctcatgag atgcttttgg
    14641 acaataaatt tggtgactca ggtgcgcgcg tggttattga ggaattcctt gaaggagagg
    14701 aattttcact ctttgccttt gtcaatggtg ataagttcta catcatgcca acggctcagg
    14761 accacaaacg tgcctacgat ggcgacaaag ggcctaacac gggtggtatg ggtgcctatg
    14821 cgccagttcc acacttacca cagagtgtag ttgatacagc ggttgacacc attgtcaagc
    14881 cagttctaga agggatgatt aaagaaggtc gcccttatct gggagttctt tacgcagggc
    14941 ttatcctgac agctgatgga ccgaaagtca ttgagttcaa cgctcggttc ggagatccag
    15001 aaactcagat tatattgcct cgtttgacct ctgactttgc tcaaaatatc acagatatcc
    15061 tggatagcaa ggagccaaat atcatgtgga cggacaaggg tgtgactctg ggtgtggttg
    15121 tcgcatccaa gggctacccg ctagactatg aaaggggcgt tgagttgcca gccaagacag
    15181 aaggcgatgt cattacctac tatgcagggg ctaagtttgc ggaaaatagc agagcactgc
    15241 tctcaaacgg cggacgagtt tatatgctgg ttaccacagt agccaccgtc aaagaagccc
    15301 aagccagcat ctaccaagaa ctatcccaac aaaaaataga aggactcttc taccgaacag
    15361 atatcggaag caaggcaatt aagtaaagat ataagaataa cgcgccgtag tcaccaaaca
    15421 cgataatggt cgtcgtggtg aaaagaccag aacagtgaat gttctggtca gggggaaact
    15481 tggagacctt aggctcaaag tttaggaatg aaaccgaagg tttgcttccg cctccatcac
    15541 ctaagaccat tatcaaaaag aaaaataaaa attcacaaaa tacgttaatg atcgtatggt
    15601 ttgcgagcgt tagcgagcta atatagaaca atcaccgccg ttgtgaaaga acgattggat
    15661 gataatccaa tcgttcaggg aaattggaag accttgggtt tccaatttag gcatgagaca
    15721 cctttggtgg ctgctgccgt ccctcacaag ctaaggtgat tgttgaaaaa gaggaaaaag
    15781 gagaagaaat gaaaccagta atttccatca tcatgggctc aaaatccgac tgggcaacca
    15841 tgcaaaaaac agcagaagtc ctagaccgct tcggtgtagc ctacgaaaag aaagttgttt
    15901 ccgcacaccg tacaccagac ctcatgttca aacatgcaga agaagcccgt agtcgtggca
    15961 tcaagatcat catcgcaggt gctggtggcg cagcgcattt gccaggcatg gtagctgcca
    16021 aaacaactct tccagtcatt ggtgtgccag tcaagtctcg tgctcttagt ggagtggatt
    16081 cactctattc tatcgttcag atgccgggtg gggtgcctgt tgcgaccatg gctatcggtg
    16141 aagctggagc gactaacgca gctctctttg ccctccgtct cctctctgta gaagataagt
    16201 ccattgcgga tgcacttgcc aactttgctg aagaacaagg aaaaatcgca gaggagtcgt
    16261 caaatgagct catctaaaac aatcggaatt atcggtggcg gtcaactggg tcagatgatg
    16321 gccatttctg ctatctacat gggccacaag gttatcgcgc tggatcctgc ggcggattgc
    16381 ccggcctctc gtgtggcgga aatcattgtg gcaccttata acgatgtgga cgccctccgt
    16441 cagttggcag accgttgcga tgtcctcact tacgagtttg aaaatgtcga cgctgacggt
    16501 ttggatgccg ttatcaagga tggacaactc cctcaaggga cagatctgct ccgcatttcg
    16561 caaaatcgta tttttgaaaa ggactttttg tcaaacaagg ccaaagtcac cgttgcacct
    16621 tacaaggttg tgacctcaag ccaagatttg gcagatatcg acttgtcgaa aaactatgtc
    16681 ctcaagactg cgactggtgg ctacgatggt catggacaaa aggttattcg ttcagaagca
    16741 gacttggaag cagcctatgc gctagcagac tcagcagact gcgtcttgga agaatttgtc
    16801 aactttgacc ttgagatttc tgtcatcgtg tcaggaaatg gcaaggaggt gacgtttttc
    16861 ccagttcagg aaaatatcca ccgcaacaat atcctgtcta agaccatcgt acaagcccgc
    16921 atttctgaaa gtctagcaga caaggctaaa gctatggcag tgcgaatcgc agaacaactc
    16981 aacttgtctg gaactctctg tgtggaaatg tttgcgactg atgatgacgt tattgtcaat
    17041 gaaatcgccc cacgaccaca taactctggc cactactcga tcgaagcttg cgacttctcg
    17101 cagtttgata cccatattct gggtgttctg ggagcgcagt tgccagctat caaactccat
    17161 gcgccagctg tcatgctcaa tgtcctaggt caacacgtcg aggctgctga aaagtatgtt
    17221 gcagaaaatc caagcgccca cctccacatg tatggtaaaa tagaagcaaa gcataatcgt
    17281 aagatgggac atgtgacctt gtttagtgat gtaccggata gtgtggatga gcttggggaa
    17341 gggattgatt tttaggacaa gtctatgata caaattatcg ttaatacatt tattgaaaag
    17401 gataagactg gagcagttgt tgaagtgttg tatgccagtg ctgaccaaga taaggtacaa
    17461 gctaaatatg aagaactagc tgcacaatac cccgaaaatt atttagctat ctatgatgta
    17521 ccgctggata cggatttgaa tacactagat cattacccgt ctgtgtttat tggaaaagag
    17581 gagtttgagt agaaatcttg gtttacctag atagcttatt cccaacagct taagaagaaa
    17641 ggaaaaatta acacatgatc aaccgttact ctcgccctga gatggcgaat atttggagtg
    17701 aagaaaataa ataccgtgct tggcttgagg tggaaatcct ctctgacgag gcatgggctg
    17761 agttggggga aatccctaag gaagatgtgg ctttgattcg caagaaggcg gactttgaca
    17821 tcgaccgtat tttggaaatt gagcaggaga cgcgccacga tgtggtggct ttcacgcgtg
    17881 cggtttctga gactcttggt gaagagcgca agtgggttca ctatgggtta acttctaccg
    17941 acgtggtgga tactgcttat ggttacctct acaagcaggc caacgacatc atccgtcgtg
    18001 accttgaaaa cttcactaat atcatcgctg acaaggccaa ggagcacaag ttcaccatca
    18061 tgatggggcg tactcatggt gtgcacgctg agccgacaac ctttggtctt aaattggcaa
    18121 cttggtacag cgaaatgaaa cgcaatatcg agcgcttcga gcatgcggct gctggtgtag
    18181 aagctggtaa gatttctggt gcggttggga actttgccaa tatcccacca tttgtagagg
    18241 agtatgtctg cgataaactt ggcatccgtg cccaagaaat ctctacacaa gtccttcctc
    18301 gtgaccttca cgctgagtac tttgcggttc ttgccagcat tgcgacttca atcgaacgta
    18361 tggcgactga gattcgtggt ctacaaaaat ctgagcaacg cgaagtagaa gagttctttg
    18421 ctaaagggca aaaagggtct tcagcaatgc ctcacaaacg caacccaatc ggttctgaaa
    18481 atatgactgg tctggcgcgt gtcattcgtg gtcacatgat tacggcttat gaaaacgtcg
    18541 ctctctggca cgaacgcgat atttctcact catcagctga gcgtatcatc acaccagata
    18601 cgaccatttt gattgactac atgctcaacc gttttggaaa tatcgtcaag aacttgacag
    18661 tcttcccaga aaatatgatc cgaaacatga actcgacttt tggtcttatc tttagccaac
    18721 gggctatgtt gacattgatt gaaaaaggca tgacccgtga gcaagcctat gacttggtgc
    18781 aaccaaaaac agcctactct tgggacaacc aagtagactt taaaccactt cttgaggcag
    18841 attcagaagt aacatcacgt ctcacacaag aagaaatcga tgaaatcttc aacccagttt
    18901 attacaccaa acgagtggat gatatctttg aacgtcttgg actaggtgat taattaaaaa
    18961 ataaacagcg agcttcaatc tcgctgttta ttttttatcg aaaagactta gtcttctttt
    19021 cttttagtga gtccataggc tgctagtgtg gccatgagtc ctgcgactac tagtcctgca
    19081 gaatcgtgag ttcctgtttc aggaagtttt ttctctatta ccacaggagc tggatcttga
    19141 ggaagagctt tgctttcctc agcaggagct ggttggctta ggatttctag ttttggtttt
    19201 tcttcagcaa tagcggcttg tccgttttca tcgcctacat gtgttaccat agttccgact
    19261 tcgactattt gagtaacggc ttcctgtgct acgacactat ttacaagtgt tttcacttcc
    19321 ttaccatcgg cagaagtgct cacagagtag aagttgctac gatgtccatt gacgccctta
    19381 gtaatgactt gtgtttttcc tttgagtaag agtggatttt cacgagtcac tgtggtaaat
    19441 ggaatttctt cttcttggat atccagtcta ggttttacct cagtagttgg tgcaagacca
    19501 ctttcatcac ccttgtgagt tacaggagcg ccaacttcaa ccacttggtt tataacttct
    19561 ttggttacct ggctatcaag gactgtttct gttgtttttc cattttcagt gagtacagag
    19621 atgtaatgag ttcgttcacc tttgactcct gctgtgataa tattttcctg accagctggg
    19681 aggttaggat tttctttctt gataacttca aatggaattt cttcagttct tgtgatgagt
    19741 tctggtctgg tttcaacatt ggcagccact tcattttcat ctaggcttcc tgaatgagtt
    19801 gcagctggtt tgaggccttg aagagcggct tttaggttgg ctacaagtgt gtcaagctca
    19861 gcttgtttat tacggttgag gttgtaattt agagctgttt tagctgcgtc aagggcctca
    19921 agactttctt tactatatcc ttctaagttt gtaggaattt tagctaattc ttcgcggaga
    19981 gcattataat tagcacggaa gtagtctttg ttgtggtctg caaaggcagt catgagttca
    20041 aagatttcct cttccttgta ttcagcgctt ggtctatctg cccagattga aagcatactt
    20101 ccgactgttg gaagatctac ttcaggatat ttggtagaag ctagttgatt gaatggaact
    20161 ttaccagaat tttcaactgc ttttgaaaga gggtaagctt catcttgttt gtgattgcct
    20221 ataacataat accagtcacc gttggtattc aagaacttat aacccttgct tgctaggtat
    20281 tgaggtgatg cgaggttgta tccccaccag cctttagacc agtaagaaat caagacatct
    20341 ttgtcaaact gaacatcgtc cttgtcttca tagtagaagc catcgttgaa ggccattggt
    20401 tgaagccctc tttctttggc catagctgcg agggtgttgg catattcggc aaatttgcca
    20461 tagagttgat accacttgag gtagtaccag ccttgggcac tagtcgcatc gttggcgtat
    20521 tcgtcagtac caaagttgaa aatctttgtt ttacctgcaa agaagtccat gtatttaccg
    20581 atgagggctt ttacaaagtt catcgcttct tcgtttttca agtccatagt tgtttttgaa
    20641 actttatcaa agtgggcttg aggattttta atacctaatt tttccatggc aaccagcata
    20701 gcatccatgt gacctggact gttaatagct gggatgagac caatgtcctt agatttagcg
    20761 tattcaatta gctctgtcac ttctgcctgt gttagtgcag taccgtttgg atcgtcgtag
    20821 taagctttag ttccttcgat aatagctttt ttaacgtcat cactagcata ggtttttccg
    20881 ttggcagtaa tggtcatatc attgagtaga aagcgaagtc cgtcatttcc tagaaggaga
    20941 tggacatcag aatatccgag ctcactggcc ttgtctacga tgcgtttgag ctggtccaga
    21001 gtaaagtatt tgcgtccagc atcgattgag attaccttgt ttttggcaag tttttcaacc
    21061 tcacgtttag cttcttcttc tttttgagct tcaggcgtga gggtcaagtt gttgacagtt
    21121 tcttgaagtt tagcaatggc tttatcaatc gtatcttgtt gggcacggct aaggttgcta
    21181 tcgagagagc gaatagcttt ttcagcttct tttacggccg tgacgctttc tgcagtataa
    21241 cggttcaggt cttttggtac ctcgttaagt gcttgctctg cagattcata atcagctgcg
    21301 aagtattcag cgttggcatt tgcaaaatga cgcatgagtt tgaagaggcg tgatggtgaa
    21361 taacgtgcag atggagtgtc agcccaagca gctaccatac caccgatgat tgggatatca
    21421 gctccttctg tttttggtac agaagtgatt ggtgtgtttt taataccatt gagcccctga
    21481 tcgagattgt accagccttg gccatcagcg tttcgtccaa gaacgtagta ccaagcatca
    21541 ttggtattaa ggatttggtg acctttttca gctagtagtt tagaagaagc gacatcgtag
    21601 cctccccaac caccagtcca catagaaacg atgatgtctt tgtcaaaact accaaagctt
    21661 gtgtcgctat tgtagtagat accgtcgtta aaggccattg gtttgagacc gtgcgatttt
    21721 acaatacgag cgaggtcatt ggcgtaggca ataaattttt catagccttt tacagggtag
    21781 ccttcgtttg gatagtattt atcagcttga agcacactcc aacctttagc atctgtcgca
    21841 tcattggcat attcatcaag tccgatgttg aagatttcag tctttttcgc gaaataagca
    21901 gcatacttgt cgataagggc ttttgtaaaa gcgacagctt gttcgttgtc aagatcgaca
    21961 gtacgggctg atttcttccc aaaatagcta aagttagggt tttggattcc caattctttc
    22021 atggcattga gaatcgcatc catgtgtcca ggactattta ctgtcggaat gagaccgata
    22081 cctttatctt tggcatagtt aatcagatct gtcatttgac tttctgttaa gtgattgccg
    22141 tttggatcgt tgtaataatc atttgtacct ttttcaatgg cgcgtttgac atcgtcactg
    22201 gcataggtct tgccgttagc tgtgatgctc atatcgtcca acatgaaacg gagtccatca
    22261 tttccgacta ataggtgtaa atcagtgtag ccataatgtt tcgctttatc gatgatttcc
    22321 ttgagctgtt ctggtgagaa atatttacgt ccagcatcaa tagaaacaat tttctttttc
    22381 gctagttttt catttacagt tgcagcacgt tcctttcctg tctctgtttc cggtttgtca
    22441 gcctctgctt tcgcttcatc ttttttagct ggtttatcct tgtcagtctt gtctgtattt
    22501 gactctttag aatcaacctc tttcgcttct tcctttttag ggctagcttc ttcttccttt
    22561 ttattagcag tttctttttc agcagaagtt ggagttacca cctctgcttt atcactagga
    22621 gttgaactaa cttcctcttg tggtttttct tctgtttttg gaagactagc taccttatca
    22681 gtagctggag tttctgtttc tacagttttt ggagcttctg gttgaagctc tgctttaggt
    22741 gtttcctcag tccgattttc ggatgattga ggggaatcag aaaccgtatg gatggtcggt
    22801 tggttttctg tagtagtagg agtaactcca tcggctgcaa cagtctgtgc ttggaaggca
    22861 aatccaatta gaacagaagc tgctcctaca gcgtatttac gaatagaaaa acgctgttgt
    22921 ttttcatgtt tcattgcaaa acctcctgat tgcattgtta tattgatagc gattatataa
    22981 atcaacgcct ttattttatt tcttatatta acgagagtca agaggagatg acaaaaaact
    23041 ataataagta taaaaaaata taaaatttaa acttaagatt tcagattggt cgaaaaatac
    23101 atatatatat ctagtataat ttttggttct atttctataa aatattccac aaattataga
    23161 atcttccaaa aataggtaag cgctaccttt ttggtgtggt ataataagca tagaaaaagc
    23221 ccgagcgatt aggctcaggc tttcttctta gtgatcacgg tcacatgaga tgaatttaat
    23281 cttgtagtaa tcagatcgtt tgtaagtttc actgtattct aaaacttggc cagttgattc
    23341 gagtttggtg attttagttt gtaggacagt agggaattgt tcatcgactc cgaggactga
    23401 agccgcatgt tctggagttg gaaagactat ttcgttgatt tcttcaaagt gttcatcatt
    23461 catgtgaatg tggtagtcta acttgaaacg attatagata gaactatagt attcaaggtt
    23521 tggataattt gcgttgatat attgttctgg gatgtaggat gtatggtaga tataaacgac
    23581 accgtttgat tcgcggatac gttcaatctt gtagtagaat tgatcgccgc gtagacccaa
    23641 tttttccaag taaacaagct tgtttccgcg ttcaattgaa agaacagtta ccttatcatc
    23701 tttagcattg aagagttcaa tatctgaaaa ctctacaagc ttgtgtttgc gtgcacgtga
    23761 aacgaaggtt ccttttcctt gttggcggac aatatagcca tctttggcaa ggtcgtttaa
    23821 ggcgcgaaca actgtgatag agctgacatc gtacattgaa atgagttctg cttcagtgta
    23881 aaatttatct ccactgctaa actgcccaga gatgatttta ttttttaatt cgtcttttat
    23941 gtattgatat ttgggaattg ccatattttt acctctattt tccttctcca tagaaaattt
    24001 taccataaaa gcgaaaaaaa tagtagtctt ttgaataaaa aattagaata atagtttgaa
    24061 aaattaagcg atatagggat tgctcatatt ttatcaatgt attctataca aaattatttt
    24121 aggtcctata ttccgaattt aaagagcgtt tgaaatgggt ctaaaaatgt ttgaaactaa
    24181 ttgaatgtgt gtataatagt aaaaatgcag taaatatccc gacaatttct attgacaatt
    24241 caaacagatt ggtttataat taatataaca acaaatgaaa tcgcaaactt tcgcggtcgg
    24301 aaggtagttt tatgacacga tttgagatac gagatgattt ctatctcgat ggaaaatcat
    24361 ttaagatttt atctggtgcc attcattatt ttagggttcc tccagaggat tggtatcatt
    24421 cgctctataa cttgaaggct cttggtttta atacggtaga gacttatgtt gcttggaatt
    24481 tacacgagcc tcgtgaaggt gagtttcatt ttgaaggtga tctggattta gagaaatttc
    24541 tccaaatagc gcaggatttg ggtctctacg caattgtgcg tccgtctcca tttatctgtg
    24601 cggaatggga attcggtggc ttaccagctt ggctcttgac caagaacatg cgaattcgct
    24661 catccgaccc agcatatatc gaggcagttg gtcgctacta tgatcagtta ttgccaagac
    24721 tggtgcctcg tttgttggac aatggtggca atattctcat gatgcaggtt gaaaatgagt
    24781 atggttctta cggagaagat aaggcttacc tgagagcgat tcgacagcta atggaagagt
    24841 gtggcgtaac ctgtcccctc tttacatcag atggtccatg gcgagctact ctgaaagctg
    24901 gaaccttaat tgaagaggac ctctttgtaa caggaaactt tggttctaag gcaccttaca
    24961 acttttcgca gatgcaggaa ttctttgatg aacatggtaa gaaatggcca ctcatgtgta
    25021 tggagttctg ggatggttgg ttcaatcgct ggaaagaacc gattatcaca cgggatccta
    25081 aggaattggc agatgcagtt cgagaggttt tggaacaagg ctctatcaat ctttacatgt
    25141 tccacggtgg tacaaacttt ggtttcatga atggttgctc agctcgagga actttggacc
    25201 tgccacaagt tacgtcttat gattacgatg cccttctgga tgaagaagga aatccaactg
    25261 ctaaatatct tgcagtcaag aagatgatgg caacacattt ttcagagtat ccgcagttgg
    25321 aaccactcta caaagagagt atggagttgg atgctattcc actagttgaa aaagtttctt
    25381 tgtttgaaac cttagatagc ttgtcaagtc ctgtagaaag tctctatcct caaaagatgg
    25441 aggagctggg acaaagttat ggctacctac tttatcgaac agaaacaaac tgggatgcag
    25501 aagaagaaag acttcgtatc attgatggtc gagatagggc ccagctgtat gtcgatggtc
    25561 agtgggttaa aactcaatat cagacagaga ttggggaaga tattttttat caaggtaaaa
    25621 agaaagggct atctaggtta gatatcttga tagaaaatat ggggcgtgtc aactatgggc
    25681 ataagttctt agcggatacg caacgtaagg gaattcggac aggggtctgt aaggatctgc
    25741 atttcttact aaactggaaa cactatccac tcccactaga caatcctgag aaaattgatt
    25801 tttcaaaagg atggactcaa ggacaaccag ccttttacgc ttatgacttt acagtcgaag
    25861 agccaaaaga tacttaccta gacttgtctg agtttggtaa gggggttgcc tttgtcaatg
    25921 ggcagaatct aggacgtttt tggaacgttg gcccaactct ctcactttat atccctcata
    25981 gctatctcaa ggaaggtgcc aaccgcatca ttatctttga aacagaaggt caatataaag
    26041 aagagattca tttaactcgt aaacctacac taaaacatat aaagggggaa aacttatgac
    26101 aattgtagga tgccgtattg atggacgttt gatccacgga caagtagcca atctttgggc
    26161 tggaaaacta aatgtttcac gcattatggt tgtagacgac gaagttgtca acaacgatat
    26221 tgaaaagagt ggtttgaaac ttgcgacacc accaggtgtg aaattgagta ttttgccagt
    26281 tgagaaagct gcagccaata ttcttgctgg caaatacgat agccaacgtc tctttatcgt
    26341 ggctcgtaaa ccagaccgct tccttggttt ggtagaagca ggtgtaccac ttgaaaccct
    26401 taatgttggg aatatgtctc aaacaccaga aactcgttct attacacgtt ctatcaacgt
    26461 agtagacaag gatgtggaag acttccacaa actggcagaa aaaggtgtta aacttactgc
    26521 tcagatggtt ccaaatgatc caatttcaga ctttttgagc ttattaaaat aggaaaaaat
    26581 tttttaggag gtcattgtta tgatacaatg gtggcaaatt ttacttctca ctttgtactc
    26641 agcttatcaa atctgtgatg agttgacgat cgtttcatct gcaggttccc ctgtatttgc
    26701 tggtttcatt actggtttaa tcatgggaga tgtgactact ggtttactta tcggtggtaa
    26761 cttgcaactg ttcgttcttg gggttggtac cttcggtggt gcttctcgta tcgacgcaac
    26821 ttctggtgcg gttcttgcga cagccttctc tgtttcacaa ggaattgatg caccgcttgc
    26881 aattactaca atcgctgtac cagtagcagc tctcttgact tacttcgacg ttcttggtcg
    26941 tatgactact accttcttcg ctcaccgtgt ggatgctgca atcgaacgct ttgactataa
    27001 aggtattgaa cgcaactact tgcttggtgc gattccgtgg gctctatctc gtgcccttcc
    27061 agtcttcttt gcccttgctt ttggtggtgc ctttgtacaa tcagtagtag acttcgttga
    27121 agcctacaaa tgggttgcag atggcttgac actcgcagga cgtatgcttc caggtcttgg
    27181 atttgcaatc ttgcttcgtt accttccagt taaacgtaac cttcactacc ttgctatggg
    27241 atttggtttg acagctatgt tgactgttct ttactcatat gtaacaggtc ttggtggcgc
    27301 tgttgctggt atcgtaggta ctcttcctgc tgaagttgct gaaaaaattg gtttcgtgaa
    27361 caacttcaaa ggtttgtcta tgattggtat ttctatcgta ggtattttcc ttgcagtgct
    27421 tcacttcaaa aatagccaaa aagtagctgt agcagcacct tctacaccat cagaaagtgg
    27481 ggaaatcgaa gatgacgaat tctaattaca aacttacaaa agaagatttt aatcaaatca
    27541 acaaacgtag cttgtttact ttccaattag gttggaacta cgaacgtatg caagcttctg
    27601 gttaccttta catgatcttg cctcagttgc gtaaaatgta tggtgatgga actcctgaat
    27661 tgaaagaaat gatgaaagtt catactcaat tcttcaatac ttcaccattc ttccatacca
    27721 ttatcgctgg ttttgacctt gccatggaag aaaaagatgg tgtaggttca aaagacgctg
    27781 ttaacggtat caagacaggt ttgatgggac cattcgctcc tcttggggat acaatctttg
    27841 gttcacttgt acctgctatc atggggtcag tcgcagcaac tatggctatc gctggccaac
    27901 cttgggggat cttcctttgg attgcagttg cagtagcgta tgacatcttc cgttggaaac
    27961 agttggaatt tgcttacaaa gaaggggtta accttatcaa caacatgcaa agtaccttga
    28021 cagctttgat tgacgctgca tctgtacttg gtgtcttcat gatgggtgct cttgtagcaa
    28081 cagtgattaa ctttgaaatt tcttacaagt tgccaatcgg tgaaaagatg attgatttcc
    28141 aagacatctt gaaccaaatc ttcccacgtt tgcttccagc aatctttact gcctttatct
    28201 tctggttgct tggtaaaaaa ggtatgaact ctactaaagc tatcggtatt attatcgtac
    28261 ttgctttggc tctttctgcc cttggtcact ttgcacttgg aatgtaattc cttatgacta
    28321 aatcattaat tttggtgagc catggtcgct tctgtgagga gcttagaggt agcacagaaa
    28381 tgattatggg cccacaagac aacatttaca cagtagctct tcttccagaa gatggcccag
    28441 aagaatttac tgctaaattt gaagctgtta ttgaaggatt ggatgatttc ctagtctttg
    28501 cggatcttct cggtgggaca ccttgtaatg tggtgagtcg cttgatcatg gaaggtcgtg
    28561 atattgacct ttacgcaggg atgaatcttc caatggtgat tgaatttatc aatgcgagcc
    28621 ttacaggcgc agatgcggac tacaagagcc gtgctgcaga aagcattgtg aaagttaatg
    28681 acctgttagc gggcttcgat gatgacgaag atgaataata ctcttcgaaa atctcttcaa
    28741 actacgtcaa cgtcgccttg ccgtaggtat atgttactga cttcgtcagt cttatccggc
    28801 aacctcaaaa cggtgttttg agctgacttc gtcagtctta tccggcaacc tcaaagcagt
    28861 gctttgagca gcctgcggct agtttcctac agattttagt tggaactcga ttcaattcat
    28921 gtgacaacgt gaaaatcgtt agagcatttt atatagaata tacatgggaa tggagcttac
    28981 tcccattccc atatttaata gaaaaagagg aactcaatgc tacattatac aaaagaagac
    29041 ttgctcgaat tgggtgcaga aatcactacg cgtgaaatct accaacagcc tgatgtatgg
    29101 agagaagctt ttgaatttta tcaagcaaaa cgtgaagaaa ttgcagcctt tctacaagaa
    29161 atcgctgata aacatgacta tattaaggtt atcttgacag gtgctgggac ttctgcttat
    29221 gtgggagata ccttgctacc ttactttaag gaagtctatg acgaacgcaa atggaatttc
    29281 aatgctattg cgacaacaga tatcgttgcc aaaccagcaa cctatttgaa aaaagatgtg
    29341 gcaactgtcc ttgtgtcttt tgctcgtagt gggaactcgc ctgaaagtgt ggcgactgtt
    29401 gatttggcca aatccttggt ggatgagctt tatcaagtga cgattacttg tgcagcagat
    29461 ggtaaattgg ctcttcaagc tcacggtgat gaccgtaatc tcttgctctt gcaaccagct
    29521 gtctctaatg atgctggatt tgccatgact tctagcttta cgtctatgat gttgacagct
    29581 ctcttggtct ttgatcctac agaatttgct gttaagtctg aacgttttga agttgtatct
    29641 agtcttgccc gtaaagtttt agacaaggta gaagatgtca aagagctcgt tgatttagac
    29701 tttaaccgtg tcatctatct aggcgctggt cctttctttg gacttgctca tgaagctcag
    29761 ctcaagattt tggaattaac tgctggtcaa gttgcgacca tgtatgaaag cccagttggc
    29821 ttccgtcacg gtccaaaatc tcttatcaac gacaatacag ttgttttggt ctttggtaca
    29881 acgacagcct acactcgtaa gtacgacttg gacttggttc gtgaagttgc tggtgaccag
    29941 attgctcgtc gtgttgtgct tttgagtgat caagcttttg gtcttgaaaa tgtcaaagaa
    30001 gtggtccttg gttgtggcgg tgtcttgaat gatatttacc gtgtcttccc ttacatcgtt
    30061 tatgcccaac tctttgctct attgacttca ctcaaggtag aaaataaacc agatacaccg
    30121 tctcctacag gtacagtaaa ccgtgtagta caaggtgtca taattcacga atatcaaaag
    30181 taagacagtg tttatgaatt cttgacaaga ggatttgtaa attatcagat aaaccataga
    30241 ttgtcagtac gctttctatg gtttgtttgc ttgagagaaa tagtaaaagg agaacagaat
    30301 gaaagcatac acagagcgtg tatttggaaa tgttgagggc gaggatgtct tggcctatcg
    30361 atttgagaca gacggtggct accaacttga ggttatgact tatggcgcga ccatcttgcg
    30421 ctatgtcgca cctgacaagg ctggaaattt tgccaatgtt atcttgggat ttgatgactt
    30481 tgatagttat gtaggcaata gtcccaagca tggagcaagt gtaggtcctg tagcgggtcg
    30541 tattgcaggt gcgacctttg agctcaatgg taagacctat gaccttgagg ttaataatgc
    30601 tagcaactgt aatcacagtg gttcaactgg ttgggattcc agcctgtttg aagttgaaga
    30661 agtaagcgat catggcttga ctctctacac agagcgtaca gatgggacag gagggttccc
    30721 tggaaatctc aagatttgga tcagttatca cttggaagaa actggtgcct atgaaatcat
    30781 ctacaaggta acgaccgatc aggatacgct ggtcaatcca accaaccaca gctatttcaa
    30841 cttgtctggt gatttcacgc agacgattga ccgtcatgtc ttccaactaa acacagaggg
    30901 catttactca atcgctcctg acggtgttcc tgccaaaact ccagaagcca accgtgatgt
    30961 ggtcaaacac atctacaatg gtaccttgtt gaaggatatc tttgcagaag aagatgagca
    31021 aatccagctg gcatcaggtt tggatcaccc atttgccctt cctgcaggcc atgacaatgc
    31081 tggattcctt tatgaccaaa attcaggtcg cttcctgctt ttcaagacag aagctccttg
    31141 ctttgtggtc tacacagcaa actttgtgga tgaaagtgtc atcataggag gtcagccaat
    31201 gctacagcac aatgggattg ctcttgaagc gcaagcttta ccagatgcca ttcacagtga
    31261 ccttaaaggc caagtcattc ttaaagctgg tcaaaccttc accagtaaga cacgttatga
    31321 acttgttgtg aaataaaaaa gctggtcaaa aagtctttaa aattaaaata acgcatattg
    31381 tcaggtgatg aaaagtttga taatatgcgt tttattatga aatgatttac tttatattat
    31441 cctgaaatgg tgttattttt gagttatctg gcttgacttt cttgatggaa attatataat
    31501 agttgtaaca ataataatta caacgagaaa agaggaccat ttatgaccta tgaatacaag
    31561 agtcacattt atttggcaga gacagtttta aatgtaaagg atttggccag tcaaacagcc
    31621 ttttatcagc aagttatcgg tttagaaatc ttatctcaaa ctgagacaga gtccattcta
    31681 ggcattggtg gaaaagtctt ggtacagctg attcaagcac aagagagcgg agaagtgagg
    31741 gaacattatg gtctttacca tttggccatt ctcttgccca cacgcaaggc tttggctgat
    31801 gtcttgaaac acctgacaga tttacagatt cctcttgtcg gcggtgcaga ccatggttac
    31861 agtgaagcta tttacttgga agatttggag ggaaatggca ttgaactcta tcgagataag
    31921 ccagtttcca catgggatat tcgagaagat ggacgcatta tcggagtgac ggaggctctt
    31981 gcggcgcagg acatttatga gttgggggaa agagtagagc cctttatcct ggcagaaggt
    32041 acgagaatgg ggcatattca tctttccgtc aaggatagtc gaaagtccag ccagttttat
    32101 caaaaggtgt tagggctaga gaataaattc agtgtgcctg gcgctagttg gattgcggct
    32161 ggagattacc atcatcattt agcagttaac gaatggagag gaaaaggtct ggcttcgcgt
    32221 aagcaaggct taccaggttt agcctactat gtcatcgaag tcgcacataa aaaagaactg
    32281 ttaacgattg cccaacgagc acaagaagtt gacgcaccaa tcaaatggat gacatcgagc
    32341 aaattggaaa tcacagactc agatggcatc gtgacccgta ttcgtttagc aaggtaaaat
    32401 taggaatttt taataagtta taagtattgc tagtttgctt tttgaacggt ctttggtaac
    32461 agaactgcgt gagctggatg ggtgagaatt ttgacaaaga agctgattag agatataatg
    32521 aagaaaaatc gagcaaggaa aaagaaatga caaattcaaa gtatattact cgtttgaaac
    32581 gttcagaggg ccagttgcgt gggattcaaa agatgattga aggagatcgt gactgtgctg
    32641 atattgtgac gcaactgaca gcagtgaaat ctagtgtaga gcgcgtgatt gagatgataa
    32701 ttaccgaaaa tcttactgaa tgtatcaatc agcccctaga tgattctgaa gctcaaaagg
    32761 aacgcctaga gaaggctatc tgatacttga ttaaacggaa ataaatgaat gttttgagac
    32821 gatatgtgca aaggtctatt aactattaga atagtttaat gtaagtcaaa agaagttgta
    32881 aaaaatcttt aaaattaaaa aacgcatagt atcaggtatt gaaatgtact gccccccaaa
    32941 agttagattt tttctgtcta acttttgggg ggcagtacat aaaaaccttg atagtatgcg
    33001 ttttattgtg gggagactta tactcttcga aaatctcttc aaaccacgtc agtttcacct
    33061 tgccgtagat atggttactg acttcgtcag ttctatccac aacctcaaaa cagtgttttg
    33121 agctgacttc gtcagttcca tccacaacct taaaacagtg ttttgagcag cctgcggcta
    33181 acttcctagt ttgcacttta attttcattg agtattactt catcttctca tgaaattgag
    33241 tttttgccca gctcctcttg attattctac ttgaccgggc caagtattca taccaccttc
    33301 tacgttggta acggtgaggc cttgggagct gagaaattgg caggtagatg cagagcggac
    33361 tcctccttga caaataacat agtattcatg gtcaggtttg agttctttgt agccttgctc
    33421 taaggtactt aatggaagat ttttggcacc tggtgcatgt cctgcttgga attcatgtgc
    33481 ttcacggaca tcgataagtt ctagattttc attttggtat tttccataaa agtcagccat
    33541 gctgatacta gtttccatat tctactcctt ctgggttagc cattttgtat agtgaataag
    33601 cgccgtcaag gttttggacg gtaaatcctg cttgcttgag gataggctct gcgatatagc
    33661 tgcgcaaacc actgtggcag ctgacgatgt aggcttgttt tttgtccagt tcatcagctt
    33721 cctccttgac actttgtcag tttttgatac aatagtacaa aattagagga ggtgggctat
    33781 gattcagaaa catgcgattc ccattttaga gtttgatgat aatcctcagg cggttatcat
    33841 gcccaatcac gaggggttgg atttgcagtt gccaaagaag tgtgtctatg cttttttagg
    33901 tgaggagatt gaccgctatg cgagggaagt aggggcgaac tgtgttggcg aattcgtttc
    33961 tgctaccaag acctatccag tctatgtcat caactacaag ggtgaggagg tctgtctggc
    34021 tcaggctcct gttggctccg ctccagcagc ccagtttatg gattggttga ttggctatgg
    34081 tgtggagcag attatctcta ctgggacctg tggtgtccta gctgatatag aggaaaatgc
    34141 ctttctagtc cctgttcgcg ctttgcgaga tgagggagcc agttaccact atgtggcacc
    34201 ttgtcgttat atggaaatgc agccagaggc tattgctgct attgaggaag ttttggaaga
    34261 cagagggatt ccttatgaag aagtcatgac ctggacgaca gacggttttt accgagaaac
    34321 ggctgaaaag gtggcttatc gtaaggaaga aggctgtgct gttgtggaga tggagtgttc
    34381 tgctcttgcg gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt tgttcacagc
    34441 agattctcta gcggacttgg accagtacga cagtcgtgac tggggctcgg aagcttttaa
    34501 taaggcgcta gaactgagtt tagcaagtgt tcaccacctt tagttgtact ggcaaaggat
    34561 ttgttttatc ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa cgaggtcacc
    34621 ttcctcttgt cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga aaaacgtatc
    34681 atatcaggtg atgaaaactt tgatactatg cgttttatgt tggtaagatt tagagtgaga
    34741 tgaaatgata ctcttcgaaa atctcttcaa accaggtcag cttcaccttg ccgtaggtat
    34801 atgttactga cttcgtcagt cttatccggc aacctcaaaa cggtgttttg agctgacttc
    34861 gtcagttcta tccacaacct caaaacagtg ttttgagcaa cctgtgacta gctttctaag
    34921 cgatgccttg gttctcattg cctataatca aaaagagaaa ttttctcctg aaaagcatat
    34981 agagtagctg gcgttaaaag ctcctgtctt gcttttttga cctatagtca catctatcaa
    35041 gtattgttct tgcctaagct atcaataaaa aggtggcatt ttttaggctt ggtgttagta
    35101 gattttgcct tatcctatct aagtcatttc gagcttttta tggtacaatg gaaacatgtt
    35161 attcaaatta tctaaggaaa aaatagagct aggcttatct cgtttatcgc cagcccgtcg
    35221 tatttttttg agttttgcct tggtcatttt actaggctct cttcttttga gcttgccctt
    35281 tgtccaagtt gaaagctcac gagcgactta ttttgatcat cttttcactg ctgtctctgc
    35341 agtctgtgtg acaggtctct caacccttcc agtagctcac acctataata tatggggcca
    35401 aataatctgt ttgctcttga ttcagatcgg tggtctaggg ctcatgacct ttattggggt
    35461 tttctatatc cagagcaagc aaaagcttag tcttcgtagc cgtgcaacta ttcaggatag
    35521 ttttagttat ggagaaactc gatctttgag aaagtttgtc tattctattt ttctcacgac
    35581 ctttttggtt gagagcttgg gagctatttt gcttagtttt cgccttattc ctcaacttgg
    35641 ctggggacgt ggtcttttta gttccatttt tctagcgatc tcagccttct gtaatgccgg
    35701 ttttgataat ttagggagca ccagtttatt tgcttttcag accgatttac tggtcaatct
    35761 ggtgattgca ggcttgatta ttacaggcgg ccttggtttt atggtctggt ttgatttggc
    35821 tggtcatgta ggaagaaaga aaaaaggacg tctgcacttt catacgaagc ttgtattatt
    35881 attgactata ggtttgttgt tatttggaac ggcaactact ctctttcttg agtggaacaa
    35941 tgctggaacg attggcaatc tccctgttgc cgataaggtt ttagttagct tttttcaaac
    36001 agtgacgatg cgaacagctg gcttttctac gatagattat actcaggctc atcctgtgac
    36061 tcttttgatt tatatcttac agatgtttct aggtggggca cctggaggaa cagctggggg
    36121 actcaagatt acgacatttt ttgtcctctt ggtctttgca cgaagtgagc ttctaggctt
    36181 gcctcatgcc aatgttgcga gacgaacgat cgcgccgcga acggttcaaa aatcctttag
    36241 tgtctttatt atctttttga tgagcttctt gataggattg attctgctag ggataacagc
    36301 caaaggcaat cctcccttta tccacctcgt atttgaaacc atttcagctc ttagtacagt
    36361 tggtgtaacg gcaaatctga ctcctgacct tgggaaattg gctctcagtg ttatcatgcc
    36421 acttatgttt atgggacgaa ttggtccctt gaccttgttt gttagcttgg cagattacca
    36481 tccagaaaag aaagatatga ttcactatat gaaagcagat attagtattg gttaagaaag
    36541 gaaagagcat gtcagatcgt acgattggaa ttttgggctt gggaattttt gggagcagtg
    36601 tcctagctgc cctagccaag caggatatga atattatcgc tattgatgac cacgcagagc
    36661 gcatcaatca gtttgagcca gttttggcgc gtggagtgat tggtgacatc acagatgaag
    36721 aattattgag atcagcaggg attgatacct gcgataccgt tgtagtcgcg acaggtgaaa
    36781 atctggagtc gagtgtgctt gcggttatgc actgtaagag tttgggggta ccgactgtta
    36841 ttgctaaggt caaaagtcag accgctaaga aagtgctaga aaagattgga gctgactcgg
    36901 ttatctcgcc agagtatgaa atggggcagt ctctagcaca gaccattctt ttccataata
    36961 gtgttgatgt ctttcagttg gataaaaatg tgtctatcgt ggagatgaaa attcctcagt
    37021 cttgggcagg tcaaagtctg agtaaattag acctccgtgg caaatacaat ctgaatattt
    37081 tgggtttccg agagcaggaa aattccccat tggatgttga atttggacca gatgacctct
    37141 tgaaagcaga tacctatatt ttggcagtca tcaacaacca gtatttggat accctagtag
    37201 cattgaattc gtaaagaggg atgacccctc ttttttgatg cccaagatgg caaatagaga
    37261 cagaagcccc ttgtcttcta gtaaaagttc ttcaaaggct ggactttatg gtaaaataga
    37321 aagaagtgac aagagagagt aatactcaat gaaaatcaaa gatcaaacta ggaaactagc
    37381 tacgggctgc tcaaaacagt gttttgaggt tgcagataga actgacgaag tcagtaacat
    37441 ctatacggca aggcgacgtt gacgcggttt gaagagattt tcgaagagta taagaaaaaa
    37501 tcagccccct aaaggagcag attatgaagt tattgtctat cgccatttct agctataatg
    37561 cagcagccta tcttcattac tgtgtggagt cgctagtgat tggtggtgag caagttggga
    37621 ttttgattat caatgacggg tctcaggatc agactcagga aatcgctgag tgtttagcta
    37681 gcaagtatcc taatatcgtt agagccatct atcaggaaaa taaaggccat ggcggtgtgg
    37741 tcaatcgcgg cttggcagag gcttctgggc gctattttaa agtagttgac agtgatgact
    37801 ggtggatcct cgtgcctact tgaaaattct tgaaaccttg caggaacttg agagcaaggg
    37861 tcaagagatg gatgtctttg tgaccaattt tgtctatgaa aaggaaggtc agtcttgtaa
    37921 gaagagtatg agttacgatt cagtcttgcc tgttcggcag atttttggct gggaccaggt
    37981 cggaaatttc tccaaaggcc agtatatcat gatgcactcg ctgatttatc ggacagattt
    38041 gttgcgtgct agccagttct aattgcctga acatactttt tatgtcgata atctctttgt
    38101 ctttacgccc cttcagcagg tcaagaccag gtactatctg cctgtcgatt tctatcgtta
    38161 tttgattagg cgtgaggacc agtctgtcaa tgagcaagtg atgattaagt gcattgacca
    38221 gcaactcaag gtcaatcgac tcttggtaga ccagcttgat ttgtcccaag tgagtcatcc
    38281 taaaatgcga gaatatctgc tgaatcatat tgaaatcacg acggtgattt ccagtaccct
    38341 gctcaaccga tctgaaacag cggagcatct ggcaaaaaaa cgccaattgt ggacctatat
    38401 tcagcaggaa aatccaaaag tctttcaggc tattcgtaag accatgttga gccgtttgac
    38461 caaacattct gtcttgccag atcgcaaact gtccaatgtc gtctatcaaa tcaccaaatc
    38521 tgtttatgga tttaattaat ataagtgttt tataagaggg atttaagaaa aattttaact
    38581 ttttcttagt cctttttaat ttcaggagat tatactagag tcatcaaata aagaaagact
    38641 ctaaggagaa tcctatgaaa ttcaatccaa atcaaagata tactcgttgg tctattcgcc
    38701 gtctcagtgt cggtgttgcc tcagttgttg tggctagtgg cttctttgtc ctagttggtc
    38761 agccaagttc tgtacgtgcc gatgtggtca atccgacccc aggtcaagtc ttacctgaag
    38821 agacatcggg aacgaaagag ggtgacttat cagaaaaacc aggagacacc gttctcactc
    38881 aagcgaaacc tgagggcgtt actggaaata cgaattcact tccgacacct acagaaagaa
    38941 ctgaagtgag cgaggaaaca aactcttcta gtctggatac actttttgaa aaagatgaag
    39001 aagctcaaaa aaatccagag ctaacagatg tcttaaaaga aactgtagat acagctgatg
    39061 tggatgggac acaagcaagt ccagcagaaa ctactcctga acaagtaaaa ggtggagtga
    39121 aagaaaatac aaaagacagc atcgatgttc ctgctgctta tcttgaaaaa gctgaaggga
    39181 aaggtccttt cactgccggt gtaaaccaag taattcctta tgaactattc gctggtgatg
    39241 gtatgttaac tcgtctatta ctaaaagctt cggataatgc tccttggtct gacaatggta
    39301 ctgctaaaaa tcctgcttta cctcctcttg aaggattaac aaaagggaaa tacttctatg
    39361 aagtagactt aaatggcaat actgttggta aacaaggtca agctttaatt gatcaacttc
    39421 gcgctaatgg tactcaaact tataaagcta ctgttaaagt ttacggaaat aaagacggta
    39481 aagctgactt gactaatcta gttgctacta aaaatgtaga catcaacatc aatggattag
    39541 ttgctaaaga aacagttcaa aaagccgttg cagacaacgt taaggacagt atcgatgttc
    39601 cagcagccta cttagaaaaa gccaagggtg aaggtccatt cacagcaggt gtcaaccatg
    39661 tgattccata cgaactcttc gcaggtgatg gtatgttgac tc
//
LOCUS       DAYYQN010000009        37294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYYQN010000009 DAYYQN010000000
VERSION     DAYYQN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.009"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            87..1490
                     /locus_tag="ACHBRQ_000460"
     CDS             87..1490
                     /locus_tag="ACHBRQ_000460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HFM9592184.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            1605..2243
                     /locus_tag="ACHBRQ_000461"
     CDS             1605..2243
                     /locus_tag="ACHBRQ_000461"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFM9592185.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            2255..2695
                     /locus_tag="ACHBRQ_000462"
     CDS             2255..2695
                     /locus_tag="ACHBRQ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HFM9592186.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            2688..3119
                     /locus_tag="ACHBRQ_000463"
     CDS             2688..3119
                     /locus_tag="ACHBRQ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9592187.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            3110..3580
                     /locus_tag="ACHBRQ_000464"
     CDS             3110..3580
                     /locus_tag="ACHBRQ_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9592188.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            3605..4378
                     /locus_tag="ACHBRQ_000465"
     CDS             3605..4378
                     /locus_tag="ACHBRQ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9592189.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            4375..5175
                     /locus_tag="ACHBRQ_000466"
     CDS             4375..5175
                     /locus_tag="ACHBRQ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9592190.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            5234..7528
                     /locus_tag="ACHBRQ_000467"
     CDS             5234..7528
                     /locus_tag="ACHBRQ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HFM9592191.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            7538..10654
                     /locus_tag="ACHBRQ_000468"
     CDS             7538..10654
                     /locus_tag="ACHBRQ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HFM9592192.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            10737..12503
                     /locus_tag="ACHBRQ_000469"
     CDS             10737..12503
                     /locus_tag="ACHBRQ_000469"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFM9592193.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAILGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLVSMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            12649..13800
                     /locus_tag="ACHBRQ_000470"
     CDS             12649..13800
                     /locus_tag="ACHBRQ_000470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM9592194.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            13945..14829
                     /locus_tag="ACHBRQ_000471"
     CDS             13945..14829
                     /locus_tag="ACHBRQ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9592195.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            15183..15434
                     /locus_tag="ACHBRQ_000472"
     CDS             15183..15434
                     /locus_tag="ACHBRQ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HFM9592196.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            15422..15712
                     /locus_tag="ACHBRQ_000473"
     CDS             15422..15712
                     /locus_tag="ACHBRQ_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HFM9592197.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQTEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQKLMNKAVKDEVAQI"
     gene            complement(15916..16023)
                     /locus_tag="ACHBRQ_000474"
                     /pseudo
     CDS             complement(15916..16023)
                     /locus_tag="ACHBRQ_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16118..17449)
                     /locus_tag="ACHBRQ_000475"
     CDS             complement(16118..17449)
                     /locus_tag="ACHBRQ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFM9592198.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(17471..18982)
                     /locus_tag="ACHBRQ_000476"
     CDS             complement(17471..18982)
                     /locus_tag="ACHBRQ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9592199.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(19193..20140)
                     /gene="arcC"
                     /locus_tag="ACHBRQ_000477"
     CDS             complement(19193..20140)
                     /gene="arcC"
                     /locus_tag="ACHBRQ_000477"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9592200.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(20305..21321)
                     /gene="argF"
                     /locus_tag="ACHBRQ_000478"
     CDS             complement(20305..21321)
                     /gene="argF"
                     /locus_tag="ACHBRQ_000478"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9592201.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(21379..22608)
                     /gene="arcA"
                     /locus_tag="ACHBRQ_000479"
     CDS             complement(21379..22608)
                     /gene="arcA"
                     /locus_tag="ACHBRQ_000479"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9592202.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLDVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            23362..25041
                     /locus_tag="ACHBRQ_000480"
     CDS             23362..25041
                     /locus_tag="ACHBRQ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HFM9592203.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(25081..27165)
                     /locus_tag="ACHBRQ_000481"
     CDS             complement(25081..27165)
                     /locus_tag="ACHBRQ_000481"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9592204.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALQQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGISLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            27337..28617
                     /locus_tag="ACHBRQ_000482"
     CDS             27337..28617
                     /locus_tag="ACHBRQ_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9592205.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            28708..31353
                     /locus_tag="ACHBRQ_000483"
     CDS             28708..31353
                     /locus_tag="ACHBRQ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9592206.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            31437..32306
                     /locus_tag="ACHBRQ_000484"
     CDS             31437..32306
                     /locus_tag="ACHBRQ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9592207.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLTWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            32300..34180
                     /locus_tag="ACHBRQ_000485"
     CDS             32300..34180
                     /locus_tag="ACHBRQ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFM9592208.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVENILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEFYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            34594..34920
                     /locus_tag="ACHBRQ_000486"
     CDS             34594..34920
                     /locus_tag="ACHBRQ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592209.1"
                     /translation="MILWSFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSLEQYPDYDG
                     FDYEEW"
     gene            complement(34990..35833)
                     /locus_tag="ACHBRQ_000487"
     CDS             complement(join(34990..35424,35426..35833))
                     /locus_tag="ACHBRQ_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFM9592210.1"
                     /translation="MDTYQRVNRLPAKQNYEASKQLTDARFKLLVGVQRTTFEEMLAV
                     LKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADLGIHESNLIRRSQWV
                     EVTLVQSGVTISRTPLSSEDTVMIDATEVQINRPKKELANDSGKKKCHAMKAQAIVTS
                     QGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQIPRKSSKL
                     KPLTVEDKTYNHALSKDRSKVENILAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINH
                     ELGF"
     gene            complement(35840..35971)
                     /locus_tag="ACHBRQ_000488"
                     /pseudo
     CDS             complement(35840..35971)
                     /locus_tag="ACHBRQ_000488"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            36306..>37294
                     /gene="pcpA"
                     /locus_tag="ACHBRQ_000489"
     CDS             36306..>37294
                     /gene="pcpA"
                     /locus_tag="ACHBRQ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HFM9592211.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLET"
BASE COUNT        11524 a         6622 c         8124 g        11024 t
ORIGIN      
        1 acattattca agtaagcgtt cacatattga aaaaataaaa tgtggggatt ataataaact
       61 taatcaagga cgaagagaga agaaaaatgg aagcggtttt agcaatagat ttaggtgcga
      121 cttctggaag agcaatcgtt ggttaccttt ctgaaaataa actagtaatg gaagaaataa
      181 atcgcttttc taatctacct attagagtaa aagggcattt atcttgggat attgactttc
      241 tactagctaa aattcttgaa agtatccgct tggctaatac tagttacaag attttatcta
      301 tcggtattga cacatgggga gttgattttg gactgattga taatgaaggt aagctgttat
      361 tacaacctgt tcattatcgt gatgaaagaa caaagggagt gttaaaggaa atatctgaaa
      421 tgactgaatt agaaaaactg tattcagaga caggaaatca gattatggag ataaatacct
      481 tgtttcaact ctttaaggca cgtcaagaat ctcctgactc tttctataag accaataaga
      541 ttcttttaat gccagatttg tttaattatc tcttgacagg taagtttgct acagaaaaaa
      601 gcattgcttc aacaactcaa ttatttgatc ctaggagtca aaattggaat cagaatatct
      661 taaaactatt tgaattggat tcatctttac ttcctgaaat tgtttcagag ggaaatgttc
      721 ttggaaggat aaaagaggag tatggtttag gcgatattcc tgttgtgaat gtttgtagtc
      781 atgatacagc aagtgcgatt gtctcagtac ctaagacaga aggtagttta tttatttcat
      841 caggtacttg gtctttggtt ggagtggaac ttacttcacc gattcttact accgaatcct
      901 tcagttatgg atttacaaat gaagtcggta aagatggaga gattacattt ctgaagaatt
      961 gtacagggtt gtggatcata gaggaactaa gacgttcatt tgaacgaaga gggaaagcct
     1021 attcttttga tgatattagg acaatggtgg agaaagaaaa agaaaatctt cctctgattg
     1081 atactgaatc aactgaattt gcaacagaat ctgatatgca caagactttg acagaatatc
     1141 tagcttatca tcatgaaact agagagtgga cagatggaca actatttaag attgtttatg
     1201 aaagcctagc tgaaacgtat aggaaagcga tagagttact agaagaacta actcataagg
     1261 tttataagag gatatatgtg attggaggag gtgctagagc cagttacttt aaccaaatga
     1321 ttgctgatag aactggtaaa gaggttctta caggttcgac tgagggtaca gctgtgggga
     1381 atattgttgt gcagctcata gctatgggac aattaaaagg gatggaagaa gctcaccatg
     1441 ttattgagga gttcctacaa ttagagagtt attactccca aaagaattaa aaagattgag
     1501 agtttgtaaa tttgcctccc tcccccttct taagttttgt acaagaaggg gggataattg
     1561 gtgaattgaa aaatatttag tgttttgata tgaggaggac aagaatgtca gatgtaaaac
     1621 aagaattaat taagtatggc aagaagctag tagaaacaga tttgacgaaa ggaacaggtg
     1681 ggaatctcag tgttttcgat cgtgaaaaac aattgatggc aattaccccg tcgggtattg
     1741 atttctttga aatcaaagaa tccgatattg tagtgatgga tattaatgga aatgttgtag
     1801 agggagaacg cttgccatct agcgaatggt atatgcattt gattcaatat caaactcgtg
     1861 atgatatcga tgcaattatc catgctcata caacttatgc aacagtatta gcttgtctca
     1921 gagaaccact tccggctagt cattatatga ttgcagtggc agggaaagat gttcgggtag
     1981 ctgagtatgc aacatatggt acgaaagaat tggctgtgaa tgcagctaaa gcaatggaag
     2041 gtcgtagagc agttttacta gcgaatcatg gaattttagc aggtgcacaa aatttattga
     2101 atgcatttaa tattgttgaa gaagttgaat attgtgcaaa aatttattgt ttagctaaga
     2161 attttggaga gccagtagtt cttcctgatg aggagatgga tttaatggca gaaaaattta
     2221 aaacatacgg tcagagaaaa tagggaggat attaatgtta aaacatatac cgaaaaatat
     2281 ttctccagat ttattgaaga ctttaatgga aatgggacat ggagatgaaa tagtattagc
     2341 tgacgcgaat tatccttctg cctcatgtgc aaataagcta attcgttgtg atggtgtaaa
     2401 tattccagaa ttattagatt ccattctgta tttaatgcca ttagatagtt acgtcgatag
     2461 ttcaattcag tttatgaacg ttgtttcggg tgatgatatt cctaagatat ggggtaccta
     2521 tagacagatg attgaaggtc atggtacaga tcttaaaacg attacttatc ttagaagaga
     2581 agacttttat gaacgtagta agaaagctta tgctattgtt gctacaggag aaacttcact
     2641 ttatgctaat attatcctta agaaaggagt agttgttgaa agagaaaatg tttaatagag
     2701 gaattttagt tgccagtcat ggtaattttg ctagcggagc tctcatgacc gcagaaatgt
     2761 ttgttggtga gacaacaaat gatagagtta ggacattagg tttgatgcct ggagagaata
     2821 ttgtagagtt tgaacattat tttaaaaatc aagtaaatga actgttagac tcaaatcaag
     2881 aggttatcgt tttgactgac ttgattggag gaagtcctaa taatgtggct ttgtcacggt
     2941 ttttaaattt ggattcagtt gatattgtaa cagggtttaa tatccctctc ctagtggaat
     3001 taatatcaag ttatgattca aaaatcaatt tagaagaaat tgttcaaaat gctcaaaata
     3061 gtttgtttaa tgttaaacaa caacttaacg tagaggagga agaggattta tgtctataga
     3121 gtttgttcgt attgatgacc gtctggtaca tggtcaagtt gtcactacgt ggctaaaaaa
     3181 gtatgatatt gagcaagtta tcattgttaa tgatcgcatc tcagaagata aaacacgaca
     3241 atctatttta aagatttctg caccggtagg tttaaaaatt gttttcttta gtgtaaaacg
     3301 gtttgtggaa gttttaaact ctgtgccaat aaaaaagaga acaatgctga tatatacaaa
     3361 tccaaaagat gtgtatgatt ctattgaagg aaatttaaaa ttggagtacc tcaatgtagg
     3421 acagatgagt aaaacggagg aaaatgaaaa ggtaacggga ggtgtagctc taggtgaaga
     3481 agacaaatat tattttaaga aaatagttga taagggaacg agagttgaaa ttcaaatggt
     3541 tcctaatgat aaagttacaa tgttggaaaa atttttataa aaataattta aggaggtaca
     3601 gtatatgcta ttcacacaag cattactggt gacattagtt gggattattg ccactattga
     3661 ctataatgga ccgttattta tgattcaccg tccgttagtt acaagtgcaa tggttggctt
     3721 agtcttagga gatttcaccc aaggtgttct tattggttca gctcttgaat taacttggct
     3781 cggtgtaaca ggtattggag gttacactcc accagatact atttcaggtg cgattattgg
     3841 tactgcattt ggtattttgt ctggtcaagg agaaactgct ggtatcgcta tagcagttcc
     3901 aattgcagtt gctacccaac agttggatgt tcttgcaaaa actttagatg tttattttgt
     3961 gaaaaaagct gataatgatg ctaaaaacgg agattattca aagatcggtt tttatcatta
     4021 ttcaagtttg gttttaatca ctttatttaa aattgtacca attttcctag ctattatgct
     4081 tggaggggaa tatgtggcag acttgtttgc taaggttcca ccaatcgtta tgcagggact
     4141 taactctgca ggtgctttac taccttcaat tggttttggt atgcttttaa atatgatgct
     4201 caagaaaaat atgtgggtat tcttgttgat tggattcatt tgttctgtgt atggaggaat
     4261 gtcaaccatt gggatctcac tagttggtat tgcggtagca tacttctacg atatgattgg
     4321 aagtaaacca caagaaacaa cttcaagtag tgatgttgag gaggatcttg atctatgatg
     4381 aataataaag taactaaagt tgaacttaaa aaagttttca aacgaagttt tatgtatggt
     4441 tcttcatgga actatgagag aatgcagaac ctaggttttc tatatacaat tcttccagta
     4501 ttgaaaaaac tatacccaga caaagattca gcttctcctg caatgaaacg tcaccttgag
     4561 tttttcaata ctcatcaaac agcggcacca tttattcttg gagttacttc cgctatggaa
     4621 gaacaagaag gaaatgaagg tgcagcttca attactggta ttaaagttgg cttgatgggg
     4681 ccgctggctg gtctaggaga tagtttgttc tggctgacac tagttcctat ctgttttagt
     4741 attggtgcgt cttattctaa agacggcggt gctttaggta tctttatcgc cttaatattg
     4801 tttaatatta ttaatattcc tgttaaatat ttcggtttga aatatgggta tactaagggt
     4861 tctagtctta tccaagaaaa taatacaaaa ggaacattga atcgcgttac gagtatggcg
     4921 acagcattag ggctagtact agtgggtggt ttgattccat caatggttgg tattaatttt
     4981 ggattagaat ttaagcaggg ggaacttgtt atttctgttc aagaaatgat tacaaaatta
     5041 attccaggat ttatccctat ggctttgact ttattaatgt gtaaattaat tagaaaagga
     5101 aagaatccgg ttgtactaat ctttagtgtt atggctattg gagttatcct agttgtttta
     5161 ggaattttga agtagtagaa agtgtggagg tggtatttgg gatatcacct ccattttgga
     5221 agagaggtaa agagtgaaat tatggtataa gaaagctgcc tcaaattgga atgaagcctt
     5281 gccgattggg aacggtcatt taggtggtat gatttatggt tcagctacaa aagaatgtat
     5341 tcaactaaac gatgagacta tttggtatag aggaaagtca gatagaaata atccagactc
     5401 actattgcat cttaaaaaaa ttcgggaata tcttttagat ggagaaattc agaaagccga
     5461 agaattgata aagttaacaa tgtttgctac cccaagagat caaagccact atgaattact
     5521 tggggaactt tacattgagc atatagatat tcagtcttgt gctctttcat tgtatgaaag
     5581 agagctagat ttagatacag ctatttctaa tgttgtgttt gagcctaata gttgtaattt
     5641 acaaataaaa agagaatatt ttacgagttt taataagaat attttatgtt gccgtatagt
     5701 gtcatcagtt caaaacacat taaatttaaa cattaatttg ggtagaaata aacggtttaa
     5761 tgatgaagta tctaaactgg attcaagtac aattttaatg tcggcctctg ctggaggtag
     5821 aaaaggtgtt cagtttaaag tggtatgtca ttctaaggtt acggatggtg aagtaagtgt
     5881 attgggagag acaatagtta ttcggaatgc tacagaggta tttctttatc tcaaatcaat
     5941 gacggattat tggggaaata tagatatttc ttctcttcag ggagaattta gtagtattga
     6001 ttactttaca gaaaaagatg aacatgtaaa aaaatatcag gagcaattta atagagttga
     6061 ttttaaacta gactatagta aagattgtct tagcattcca acgaatctac ttcttgaaaa
     6121 cactaaaaag tatagtaact acttgactaa cttgttattt cattatggaa gatatctgtt
     6181 aatatcgtct agtcaaccga atggtttacc tgccaatctt caaggaatat ggtgtgatga
     6241 attaaatcca atttggggtt ctaaatatac gattaatatt aatactcaaa tgaattattg
     6301 gatggtaggt ccatgtgatt taccagaagt agaatatcca ttatttgata tgctcgaaag
     6361 aatgagagaa ccgggaagac taaccgctaa gaaaatgtat ggagctagag gttttacagc
     6421 acatcataat acggatggtt ttggcgatac ggctccccaa tctcatgcca tgggggctgc
     6481 aatttgggta ttaactattc catggttatg tactcatatt tgggaacact atttatattt
     6541 ccaagatgag cgtattctta cggaacattt tgaaatgata aaagaagcat ttcttttctt
     6601 tgaagattat ttatttgagg tggatggcta cttgatgaca ggtccaagtg tctcaccgga
     6661 aaataaatat cgcttaaaaa atggtattga aggaaatgct tgtctatcat ctacaattga
     6721 taatcaaatt ttaagatatt tttgtgattc atgcattgga attgcaaaac aattaggaga
     6781 caattcggat tttattagtc gtgtgaagga gttaaaaaag aaattaccta gaacaaaaat
     6841 aggtagtaat gggcaaatac aagaatggct agaggattat gaagaagtag agcctggtca
     6901 tagacacatt tcacctctat ttgggcttta tccttataat gagattgata ttcataaaac
     6961 tccggaatta gcagaagcag ctaaaatcac tatcaatagg agattatcaa acgctaactt
     7021 tttatcttca caggataggg agcaagcgat taataattgg ttagtaagtg gtttgcatgc
     7081 tagtacacaa acaggttgga gtgctgcatg gctgattcat ttttttgcga gactatatca
     7141 aggtgaacct gcttataacc agattaatgg tttgttaaat aatgcgactc ttggcaattt
     7201 atttcttgac catccaccat ttcaaattga tggtaattta ggtttggtga gtggaatttg
     7261 tgaattatta gtacagagcc atcataattg gttatcacta attccagctt taccttctgc
     7321 ttggtcagaa ggagaagtga aaggtttcag agtaagagga ggatataagg tatcgtttgc
     7381 ttggaaaaat ggggatataa cattcctaaa attggaagga ggaaacaaag atcaaaaagt
     7441 aagagtaaga atatatggca aaaatactga tgtacaaaat attgaattgg tatttaattc
     7501 agaaaaaatt attgagttaa atttttaggt ataagtcatg aataaagaaa aaataaaaag
     7561 aaaattaatc acaatactgt ttgtatgtat tgggatgtta tgttttggat tgttagcagg
     7621 agttaaggct gataatcgtg ttcaaatgag aacgacgatt aataatgaat cgccattgtt
     7681 gctttctccg ttgtatggca atgataatgg taacggatta tggtggggga acacattgaa
     7741 gggagcatgg gaagctattc ctgaagatgt aaagccatat gcagcgattg aacttcatcc
     7801 tgcaaaagtc tgtaaaccaa caagttgtat tccacgagat acgaaagaat tgagagaatg
     7861 gtatgtcaag atgttggagg aagctcaaag tctaaacatt ccagttttct tggttattat
     7921 gtcggctgga gagcgtaata cagttcctcc agagtggtta gatgaacaat tccaaaagta
     7981 tagtgtgtta aaaggtgttt taaatattga gaattattgg atttacaata accagttagc
     8041 tccgcatagt gctaaatatt tggaagtttg tgccaaatat ggagcgcatt ttatctggca
     8101 tgatcatgaa aaatggttct gggaaactat tatgaatgat ccgacattct ttgaagcgag
     8161 tcaaaaatat cataaaaatt tggtgttggc aactaaaaat acgccaataa gagatgatgc
     8221 gggtacagat tctatcgtta gtggattttg gttgagtggc ttatgtgata actggggctc
     8281 atcaacagat acatggaaat ggtgggaaaa acattataca aacacatttg aaactggaag
     8341 agctagggat atgagatcct atgcatcgga accagaatca atgattgcta tggaaatgat
     8401 gaatgtatat actgggggag gcacagttta taatttcgaa tgtgccgcgt atacatttat
     8461 gacaaatgat gtaccaactc cagcatttac taaaggtatt attcctttct ttagacatgc
     8521 tatacaaaat ccagctccaa gtaaggaaga agttgtaaat agaacaaaag ctgtattttg
     8581 gaatggagaa ggtaggatta gttcattaaa cggattttat caaggacttt attcgaatga
     8641 tgaaacaatg cctttatata ataatgggag atatcatatt cttcctgtaa tacatgagaa
     8701 aattgataag gaaaagattt catctatatt ccctaatgca aaaattttga ctaaaaatag
     8761 tgaggaattg tctagtaaag tcaactattt aaactcgctt tatccaaaac tttatgaagg
     8821 agatgggtat gctcagcgtg taggtaattc ctggtatatt tataatagta atgctaatat
     8881 caataaaaat cagcaagtaa tgttgcctat gtatactaat aatacaaagt cgttatcgtt
     8941 agatttgacg ccacatactt acgctgttgt taaagaaaat ccaaataatt tacatatttt
     9001 attgaataat tacaggacag ataagacagc tatgtgggca ttatcaggaa attttgatgc
     9061 atcaaaaagt tggaagaaag aagaattaga gttagcgaac tggataagca aaaattattc
     9121 catcaatcct gtagataatg actttaggac aacaacactt acattaaaag ggcatactgg
     9181 tcataaacct cagataaata taagtggcga taaaaatcat tatacttata cagaaaattg
     9241 ggatgagaat acccatgttt ataccattac ggttaatcat aatggaatgg tagagatgtc
     9301 tataaatact gaggggacag gtccagtctc tttcccaaca ccagataaat ttaatgatgg
     9361 taatttgaat atagcatatg caaaaccaac aacacaaagt tctgtagatt acaatggaga
     9421 ccctaataga gctgtggatg gtaacagaaa tggtaatttt aactctggtt cggtaacaca
     9481 cactagggca gataatccct cttggtggga agtcgatttg aaaaaaatgg ataaagttgg
     9541 gcttgttaaa atttataatc gcacagatgc tgagactcaa cgtctatcta attttgatgt
     9601 gattctatat gacaataata gaaacgaagt tgctaagaaa catgttaata atttgtcggg
     9661 tgaatctgtt agtctagatt tcaaagaaaa aggagcaagg tatattaaag ttaaattact
     9721 aacgagtgga gtgcctttga gtttagcaga agtagaggtt tttagagaat cagatggtaa
     9781 gcaatctgaa gaggatatag ataaaataac agaagataaa gtagtctcta caaataaggt
     9841 agctactcaa agttcaacca attatgaggg tgtagctgct ttagcagttg atggtaataa
     9901 agatggagat tacggacatc attcggtgac tcatactaag gcagattcta acgcttggtg
     9961 gcaggtcgat ctgggagaag agtttacggt ttctaaagtt gatatttata atagaacaga
    10021 tgccgaacct cagcgtttat ctaattttga tgttattttt ctatcttcat caggagaaga
    10081 agtttttaga agacattttg ataaagtagt tgatggtttg ttatctttaa aagtaccttc
    10141 tgtaggggct aagctagtca aaatagaatt aaaatcagca gctattccgt taagtttagc
    10201 ggaagttgaa gtctatggtt caaagagaac tccgaagaaa ctttctaata ttgcattaac
    10261 aaaagaaact cgacagagtt caacggatta caatggtttt tctcgtctag cagttgatgg
    10321 aaataaaaac ggagattatg gtcatcattc agtgactcat accaaagaag attctccttc
    10381 atggtgggag atagatttag cacaaaccga agaattagaa aagttaatta tttataatag
    10441 aacagatgct gaaattcaga gattatcaaa ttttgatatt attatatatg attcaaatga
    10501 ttatgaagtt tttacacaac atattgacag tttagaaagc aataatctat ccatagactt
    10561 aaaaggactg aagggaaaaa aggttagaat ttctttgaga aacgcaggaa ttcctttaag
    10621 tttagcagag gtagaggttt atacttataa gtaattttaa aaattatcac ccaggctacc
    10681 gtaaatataa tggagatggt agtatgaaag aaacagaaaa ataagaggaa aatagtatga
    10741 ttcaacatcc acgtattggg attcgtccga ctattgatgg tcgtcgtcaa ggtgtacgcg
    10801 aatcacttga agtgcaaaca atgaacatgg ctaaaagtgt ggcagatttg atttcaagca
    10861 cattgaaata tccagatggg gaacctgtgg aatgcgtgat ttctccatct actattggcc
    10921 gtgtaccaga ggctgcagct tcccatgagt tgtttaaaaa atcaaatgtt tgcgcaacaa
    10981 ttacagttac accatgctgg tgttatggta gtgaaactat ggatatgtct ccagatattc
    11041 ctcatgctat tttgggattt aatgggacag aacgcccagg agctgtctat cttgcagctg
    11101 tactagcttc acatgctcaa aaagggattc cagcctttgg gatttatgga agagatgttc
    11161 aggaagctag tgacacagat attccagaag atgtcaaaga aaaactttta cgctatgcgc
    11221 gtgcagctct tgcaactggc ttgatgagag acactgctta cctatcaatg ggtagtgttt
    11281 cgatggggat tggtggttct attgtaaatc cggatttctt ccaagaatac ctaggaatgc
    11341 gaaatgaatc ggtagatatg acggagttca cgcgccgtat ggaccgtagt atttacgacc
    11401 ctgaagagtt cgaacgtgcg ctcaaatggg tgaaagaaaa cgtaaaagaa ggattcgacc
    11461 ataaccgtga agaccttgtt ttaagccgtg aagaaaaaga tagacaatgg gaatttgtta
    11521 ttaagatgtt catgattgga cgtgacttaa tggttggtaa cccaagactt gctgagcttg
    11581 gttttgagga agaagcagtt ggtcaccatg ctttagtagc tggtttccaa ggtcaacgcc
    11641 agtggacaga tcatttccca aatggggact ttatggaaac tttcctcaat actcagtttg
    11701 actggaatgg tattcgaaaa ccatttgtat ttgcgacaga gaatgattca ctaaatggtg
    11761 tgtctatgct ctttaattat ctattaacaa atactccaca aatctttgct gatgtgcgta
    11821 cttattggag cccagaggct gttaaacgtg taacgggaca tactttagag ggtcgtgctg
    11881 cagctggctt cttacatcta atcaactctg gttcttgtac attggatggt acaggtcaag
    11941 ctactcgaga tggcaaacct gttatgaaac cattctggga gttggaagaa agtgaagtgc
    12001 aggctatgct tgaaaataca gacttcccac cagcaaaccg cgaatacttc cgtggaggag
    12061 gattctcaac tcgtttcttg acgaaggggg atatgccagt aacaatggta cgtctcaatc
    12121 ttctaaaagg ggttggtcca gtgctacaaa ttgcagaagg ttatacactt gaacttcctg
    12181 aagatgttca ccatacttta gataatcgta cagatccagg atggccaact acttggtttg
    12241 ctccacgttt gacaggaaaa ggtgctttca agtctgtcta tgacgtcatg aataattggg
    12301 gagctaatca cggagccata acatatggac acattggagc agacttgatt accttggttt
    12361 ctatgttgag aattcctgtc aatatgcata atgtacctga ggaagatatc tttagaccta
    12421 aaaattggtc cttatttgga acagaagatc tagaatcagc agactatcgt gcatgtcagt
    12481 tgttgggacc actacataaa taaaacttgt ttatatagga ggtgaactta cgtccctcct
    12541 atccttttaa aaagatttgt taaacaattc acaaataatt gaaaacgaat acaaaaagta
    12601 atataatgat gttaaataga tagcgcggag gcgcaggagg aaaattatat ggctacattt
    12661 tatgttccgg cagtcaacct tattggtaaa ggtgttgtaa atgaagtggg tccttatatc
    12721 aaggaacttg gctataaaaa ggcacttttg gttacagata agtacatcga aggcagtgat
    12781 attttgccta aggttttaaa accactggat acagaaggaa tcgaatatgt catctttagc
    12841 gatgtagagc caaaccctac ttgtaaaaat gtcacagatg gggtagctgc tttgcaagaa
    12901 catggctgtg actttatcat cagtcttggc gggggttctc cacaggatgc agctagttgt
    12961 atttctatca tggctacaaa tggtggaaaa ccacaggatt atgaagggct tcataagtct
    13021 gctaaaaaag gcttgccagt tgtggctatc aatacaacgg caggaacttc tgcagaaatc
    13081 actattaact atgtgattac tgatgaagaa cgcaaggtta agatggtaat ggttgacaag
    13141 aatagccttg ctcttatctc tgttaatgac ccggaactca tgctttccaa acctaaaggc
    13201 ctgactgctg ctactggtat ggatgctctg actcacgctg ttgaagcttt ggtaacacct
    13261 ggtgcttatg atgtaaccaa gaaactgtct attggtgcta ttgagcttat caaggaatat
    13321 cttcctcgtg ctgtagaaaa tggacatgat attgaagcgc gtgaaggtat ggtcaatgcc
    13381 atcttccttg gtggtatgag ctttaataat gctggtcttg gttatgttca ctcaatggct
    13441 caccaactcg gtgcagtata taatttgcca catggcgtgt gctgtgccat gttgctacca
    13501 gttatagaac gtgaaaatgc taaacgtgta ccagaagctt tccgcaatgt tgccaaagcc
    13561 ttgggacttc atgtagaagg taaatcagat caagaatgtg ccgattatgc gattgctgag
    13621 attgagaagc tttctgagac agtaggtatt cctaagaaac tgactgaact tggtattgaa
    13681 gaaaaagatt tcgactttga atacctttct aagaatgcct tgattgatgc ctgcgcacca
    13741 ggaaatccat ttatgccaac cttagaagaa acgattgcct tttataaaga gttattttag
    13801 caagagattg gacgacaata ggacaattca cctctaatta ttgacatttc aggaaaaatc
    13861 gcagtaaaaa cggcacagcc ttctttaaat gtgctataat actagaaaaa tacttgtgga
    13921 ggttccatta tggcaatatt tttcatgatt tttctgattg tttgtgtgct cctattggtg
    13981 atagtcacac tgagtacagt ttatgtggtt cgtcagcagt cggtggcgat tattgaacgc
    14041 tttgggaaat accaaaaggt tgctaatagc ggtattcata ttcgcttgcc ttttgggatt
    14101 gactcgattg cagcacggat tcagttgcgc ttgttgcaaa gtgatattgt ggttgagact
    14161 aagaccaagg acaatgtgtt cgttatgatg aatgtagcga ctcagtaccg tgtcaacgag
    14221 cagagcgtga cagatgctta ctataaactc atacgtccag aatctcagat taaatcttat
    14281 atcgaagatg ctcttcgctc ttctgttcca aaattaacct tggatgaatt gtttgagaaa
    14341 aaagatgaga ttgcccttga ggttcaacac caagtagcag aagaaatgac cacttacggc
    14401 tacattatcg tgaaaacctt gattaccaag gtcgaaccag atgcagaagt taagcaatcc
    14461 atgaatgaaa tcaatgcggc gcaacgtaag cgggtcgcag cacaagaatt ggcggaagct
    14521 gacaagatta aaattgtcac tgcagctgaa gcagaagcag aaaaagaccg ccttcatggt
    14581 gtggggattg cccaacaacg taaggcgatt gtggatggat tggcagagtc tatcaccgaa
    14641 ctcaaggaag ccaatgttgg catgacagaa gaacaaatca tgtctatcct cttgaccaac
    14701 cagtatttgg ataccttgaa tacctttgcc tctaaaggaa atcaaaccat ctttttacca
    14761 aatactccaa atggtgtgga tgatatccga acacaaatct tgtcagccct tcgcgctgag
    14821 aagaaataat agactaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgtcttgc
    14881 cgtatagatg ttactgactt cgtcagttct atctgcaacc tcaaaacagt gttttgagca
    14941 acctgtggct agtttcctag tttgctcttt gattttcatt gagtataaag agcttggcaa
    15001 caggctcttt ttgtattgcg tttattaaga aaaacaaaaa acatcgatat acatttaact
    15061 aaaaattatt gtatgtcatc tcccttaatt aggaatgata agggaataac tagaaagatt
    15121 tgtgaataca aactatttct gatatacaaa atatacagta ataatgaatg atgggagatg
    15181 ggatgaaaga atttcaattt gagagaaagc agcgtttttc tttgaggaaa tatgcaatag
    15241 gagcttgttc ggtcttgcta ggaacgagtt tattttttgc tggtatgggt gctcagcctg
    15301 tacaggctac agaaacgagt tcagcactaa tttcaagtca ttatttggat gagcaggatt
    15361 tatctgaaaa gctgaaatct gagttgcaat ggtttgaatt agaaaacaag cttttgaact
    15421 tatgggagca ttaggttact atgaaggatt tgttccttat gtttcaaatc aatacaaaaa
    15481 ccaaactgaa gaagaaggca aaccgctatc tgataaatat attttcgaaa aaatcttagg
    15541 aaaaacatat gcagccttca aaaaagatca aattaatgag cgtgtcgaga aattaggtaa
    15601 gttaaaacct attacaataa attacaacgg aaaatcagaa gtaattgata gtaaagaaaa
    15661 attacaaaag cttatgaata aagccgttaa agacgaagtg gctcaaatat agtaaaatga
    15721 aataagaaca ggacaaatcg atcaggacag tcaaatcgat ttctaaaaat gttttagaag
    15781 gagaggtgta ctattctagt ttcaatatac tataaaatca ggcaatacca cggctaaaaa
    15841 gtttgagttt atcgaaacac cagtgcaaaa actgaaaaaa gcgatttata aagctcatct
    15901 caaagattca gatgacttta gaccagaaac atccacacca aatctttttg aaagctgttt
    15961 gaagctttgt ccttgctttc ttagttcata gatctgaact ttatcttcat aactcaattt
    16021 cataataaaa caccccaaaa gttagattta ttctgtctaa cttttggggt gcagttcatt
    16081 aatcggtcga ttttttttgg tagaaacagt tgcctgatta agttgcaagt cgatagacgg
    16141 cttcggcata aatatccata gcacggtaca aatcttctag aacggcacat tcatttgcct
    16201 gatgttctgt ctgcttcgct cctgggaata aggcgccgaa ggctacacaa tttggcatgg
    16261 tgcgagcaaa agtggcacca ccggatgaaa tagcaggact gttatcgcca gtcttttctt
    16321 ggtagatttg catcagtgtg ctaacgagtt tactgtcttt tgcgacgtat agaggcgcta
    16381 gatagtcaaa ctcttcgtag cggagttggt agttttgtgc acatcttgta agcaactcta
    16441 ctagttcttc cttatcagct aagacaggag tccgaatgtc aatacggatt tcagaacgtt
    16501 catgattgat catgagacct gcgacattaa aggataggtg accagaaggt tcatctgcta
    16561 tatcaccaaa gatttgtctt cctgtgccgt cttgacctgc ttgtgttgca agaaaactga
    16621 gagcagggtg ttcttggaga ggagcaagaa tggtagctag tcggatgaca gcattgatac
    16681 cttgactagc atccttagca tgctttggca ctccgagaac cgttacggtt tgttcagtgg
    16741 attggtaatc ataaccagct tctttgagac cgttacaaac ctgttcatag aggggacctt
    16801 ggtagttggc cttgtctggt acaacgttaa aggcgcctcc tacttcaagc tctagttgat
    16861 ccgatccagg gccatgaagt ttgacctgta gaagcccttt ttcagcatag gtcagaggaa
    16921 aagatgagtc aggtgcaaag cccatgctgg cctgttcttc gatggtattg tagcgtgcca
    16981 tgcagcgcca gagggtttcc tcatcggtac caaagataaa gcgtacgcgc tttttgaact
    17041 gaataccttg gtccagcaag ctttttactg catagagagc tgcgagcgaa gggcctttat
    17101 catcttggac accacgtccg aatacccagc cgtctttgat agttgcttca aatggcggtg
    17161 tctgccaatc tgcttcatca cctgatggaa caacatccaa atgacagaga atggccagaa
    17221 gctctgctcc ctgaccgatt tctgcatatc cgtaataacc tttagggtca agataggtag
    17281 tgaaacctat gtcttgacaa atctctaaag ttttttctag gacatcttgg attgcttgtc
    17341 caaaaggtgt tccattttct ccttcattga gtactgaagg ataggaaatc aaggttttta
    17401 atgatataag aaattcatct ttaacttgat ttgttatatc tattttcatg gaatcacctc
    17461 actcactatt ttataggaat ggaaggaagg ttccaaggag aagaagggcg atggtcacta
    17521 caataatagc gactacgagt ttgcccatga atttccacca agtaccaatg ttgatacgtc
    17581 caagtgcaag agctcccatc acaataccag atgttggtgc aatcaagttc aagacacctg
    17641 aagcagattg gtaagcagtg ataatcaagc taggacggac atttacaaat tctccaagtg
    17701 gagccatgat acccatagtt gcgctggcaa gaccagatga agatgggatc aagaatgaca
    17761 taggtagata gaagatataa gttacaacga taaagacttg tgaagataga ccgctcaagc
    17821 cttctttacc ccagttgagg attgtatcgg taatcatacc gtcgttcatg ataacttgaa
    17881 taccacgagc aatcgctacg atcaaggcaa cactgagcaa gtcagcagca ccattcatga
    17941 aggaagagat aatcttatct tctttaagac cataaataac accaatcagg atacccataa
    18001 aggcaaagag cattgcgcct tctgggaagt accaagtacc tagtgcagaa gtagatgaac
    18061 cgacaatatt accaataact ggaagaccag tcaaccaagc attaaagtca tcaaaaatgg
    18121 taacgccaag gtctgtccat ggaatgaagc tcaataccat caagatgaat gtcaacacaa
    18181 ataagaagag aactgatttt tgtttgctgc taagtgtaga ttctacagat gaagattctt
    18241 ccacgttaaa gtgtttcaaa tcttctttgc gagtactata aaccagtgac ttagtcggat
    18301 ctttttgaat cttatccgca taacggtaaa caaaccaagt actaagagca gtcaaggtaa
    18361 cccagaagat cagacgaagt acgataccgt cccctgtacc aactcccgca gtcgctgaag
    18421 caatacctgt cgcaaatgga ttcagagtag atgccaaaca gccgatttga gaaccgagca
    18481 aaataattgc aacaccagtc aggctatcaa aaccaacggc catcataact ggcacaagga
    18541 gtggatagaa agccattgtt tcttcaccca taccataagt tgtaccaccg agggcaaaca
    18601 aaggcatcag taccaaaatt aacatttttt cgcggccctt atacttcttc acgatagagg
    18661 caatccctac gtcaagagca ccagttttgt tgacaatgcc aaggaaacca ccaaccataa
    18721 ggatgaagaa ggctacatca atcgctgcgc tcgtttcttt aatgagcgaa ccttcctctg
    18781 gatgagtacc tagcatagcc cgaatcggtg ccatgaggac atcccaaatc ccttgtggat
    18841 tttgaggctg agtctcgtaa ataccttcta taaaggcccc cgcagggata aaccaagtta
    18901 gcactgccat aatagcaatg attatcaata atacggtgta agatgaaggc atcttaaacc
    18961 cttttttagc tttttcactc atttgttttt cctcctgatg tctaacaaag tgtaaaaaac
    19021 caactacttt cttaaataaa catttaagaa aacgctttct atttatattt gttttaccat
    19081 attaaagtct aaaaaactaa tatcttttga aatattgtac atgttttcaa gaataatttc
    19141 taatttgata taaagatact agaaatagaa tacatcttat tagtaaaaca acttatcctt
    19201 tttcaataat tgttccactt tcagattcaa tcaaggcgcc tagattttca agggaagtaa
    19261 taactgcttt tccttctgga cgaccattga caaaagcgat agctgcttct acttttggaa
    19321 gcatgctacc tggtgcaaac tgatcttgtt tgatatattc ttccagctgg gcaacattca
    19381 catgttccaa tttttcctgg tttggcttgt tgtagttaac aaatacataa tctacacctg
    19441 tcaaaatgat gaagaggtct gcatcaacca attctgccaa acgttgggaa gcgaagtctt
    19501 tatcaataac cgcttcgaca ccagtcaaat gtccattgtt ttctttgacg acgggaatac
    19561 cgccaccacc tgcagctacg acgacttgac cattatttaa aagagtacgg atggtttcaa
    19621 tttctttgat gtcaacaggt tttggtgagg caacgacctt acgccagcca cggccagcat
    19681 cttccttgaa agtcgctccg cttttttcgg cttctgcttt tgcttcttct tctgaataga
    19741 aaggaccgat tggtttactc aagttaacaa aagctggatc atttttatct acgacaactt
    19801 gcgttacaac agaggcaaca tttttttcga tgccttcatc caagagagca ttttgcaaag
    19861 catttttcaa ccagaaaccg atgctacctt ctgtcatagc gacaagtgag tcgagtggga
    19921 aggcagggtt cttttcagag tctgatgcca aatgttggag caagagattc ccaacttgag
    19981 gtccattacc gtgagtgata atcaaatcat ctccattttt aatcaatttt acaagatgct
    20041 tagctgtttc aactaaagct tcttgttgag cctttgctga tgggtcagaa gaaagaatcg
    20101 catttcctcc caaagctact acaattttac gatttgccat aaattctcct ttatcacact
    20161 caatagaatg cgtttagatt tcaatttaat gatttttcac atattttata agaaataata
    20221 gattaccatt acataaaaga ggaccggact aaagctatta gtcgcagccc tcatagctgt
    20281 tggtagacgg tttattatct aaaattatac tttaggaata taaaggttac caagtgtcgc
    20341 agccataaca gctttgatag tgtgcatacg gttttctgct tgatcgaagt ggcgagcgta
    20401 cttgctgcgg aagacttcgt ctgttacttc catttcttct acaccaaatt tttcagcaac
    20461 gtctttacca taaacagtgt gagtatcgtg gaatgctggc aagcagtgta ggaagatcaa
    20521 gttttcattg cctgcttttt taactaagtc catattgact tggtaaggtt taagaagagc
    20581 tacacgttct gcgaatttgt cttcttcacc cattgatacc caaacgtctg tgtaaagaac
    20641 gtctgcatct ttaactgctt catcagcatc ttcagtgatg agaacatgtg cgccactttc
    20701 tttagcaaat ccttctgcca attcaacgat ttctttttct gggaagagtt cttttggtga
    20761 gaagatgtga acattgacac caaggatagc acctgttacg agcaagctgt tggcaacgtt
    20821 gttacgtcca tcaccacagt ataccaatgt caagccttcc aagcgaccga agttttcttg
    20881 aacagtcaag tagtcagcga gcatttgagt tgggtgccat tcgtcagtta gaccgttcca
    20941 tactggaacg cctgagaatt ctgccaattc ttcaaccata cgttggctga atccgcggaa
    21001 ttcaatcccg tcaaacatac gtcccaatac tttagcagta tcttcagtag attctttttt
    21061 acccaactga atatcatttg ctccgaggta ttctgggtga gcaccaaggt cgatagccgc
    21121 agttgtaaag gctgcacgag tacgagtaga tgttttttca aataggagag cgatattctt
    21181 gccagcaagg tagtggtgtt gaatattgcg ttttttcaaa tctttcaagt gagctgaaag
    21241 accaataagg tattctaact ctgcacgggt aaagtctttt tctgctaaga agctgcgtcc
    21301 ttggaatact gaatttgtca ttttattatt tcctctttct attttttaca ttttctattg
    21361 acgaatatcg aacagcgatt acacttcttc acgttcaaat ggcatagaca tacaacgagg
    21421 tccaccacgc ccccgaacca attcacttcc gcgaatctta atcaagcgaa gcccgtattc
    21481 ttccaaaatc ttattggtca cggtattgcg gtcataaaca actaccacac caggtgcgat
    21541 ggtcaaagtg ttagaaccgt cgttccattg ttcacgcgca gctgctacga tattgccacc
    21601 accgcaacga atcaaatgaa ctttttctac atcaaggttt tgagcaagaa gttcagctaa
    21661 gtcacctttc tcttcaacga ttttaagttt ttcgttttcg taagtaactg agtaaacgtg
    21721 aaggtcgcct tcgatttctg ggtgaatagt gaacttgtca tagtctacca tagtgaagac
    21781 agtatccaag tgcatgaatt tacggttgtt agcaaattca aaggccaaaa ctttcttgaa
    21841 gccaacattt ttcttgaaga tgttgaccaa aagtttttct atagaagctg cgtctgtacg
    21901 ttgagagata cctactgcaa ggacgtcttt agaaagaact agctcgtctc caccttcgat
    21961 acgcgtatct tcttcacggt tgtagaccaa atccactttt cctccataga ttgggtggta
    22021 tttgaagata tacttaccgt agagtgtttc acggttacga gtgtctgcaa acatgtggtt
    22081 aagcgatacg gcgtttccaa ttgttgcaaa tgggtcgcga gtgaaataga ggtttggcat
    22141 cgggtcaatt gcaaatggat aatctgattc aactaagtca gttagatctt tagcttcgtc
    22201 aggaatttct ggcaattcaa ctttttgaat cccagccatt gttttttcaa ccaattcttg
    22261 gttgtccttg atgccgtgaa gcaattcacg aatagcaacc ttggtttgac gatcacggat
    22321 gttggcttcg tctaagtatt cctcgataaa ttgatcgcgg atttctggag aggtcaatga
    22381 ttcagcagcg agttgttcta ggtagagaac ctcaattcct tcatcgcgaa gagcttgggc
    22441 aaatgcatca tgttcttttt gagcatcttc caagaaagga atatcatcaa aaagaagcct
    22501 ttcaagatag tccggcaaca agttttctaa ctccttgcct ggacggtgta acataacttt
    22561 tttcagtttc ccaatttctg agaagacctg aattggatgt gaagacatgt attttcctcc
    22621 ttaaaaattt tgacttggca ccattttggg taccgcttac atgtatgtta taacgcaatt
    22681 cctaggtaat gtcaatggct ataatttctc ggaaaataat ataaattatg atatttagca
    22741 caattttatg ttattataca caacatagga tggaatatat atacaaatgc ataaacttct
    22801 attaaagagg atataaaatt tttttaaaaa atttttcaaa aaatacacaa ataaccaaaa
    22861 agtgtccaat tatcatctgg ttatcacttg gatctgtata aagagaaatg tttgtgtgaa
    22921 aggataaaaa gatgcaacat tcttttgaag atagattttg gagaagcatt tcttgctcaa
    22981 ttgtacaaat tatagaatct tttctgagta tttaggaaga gataattatc ttattgaccc
    23041 tctttttatt ttttgttata gtcgacgaga tgatagttta gaataatcac ttattcacaa
    23101 tctgtggaaa agtctggaga gtgaatggtc ttcacacaat gacctgaaag aagcctgaga
    23161 ataattatgg agagtagcat tctgagaggt gttagcagaa ccatatgaca gagctgtttg
    23221 aagagggaac attgaggagc aaaatcctga gcctaccagt tggagttgga aagagctggc
    23281 tgttagatca tggtttatta tccacaacct gtggataacc ttgtgaacaa gagaagttgc
    23341 taaagaagga gatatataac gatgaagaaa atcaaaccgc atggaccgtt accaagtcag
    23401 actcagctag cttatctggg agatgaacta gcagctttta tccacttcgg tcctaatacc
    23461 ttttatgacc aagaatgggg gactggacag gaggatcctg agcgctttaa cccgagtcag
    23521 ttggatgcgc gtgagtgggt tcgtgtgctc aaggaaacgg gcttcaaaaa gttgattttg
    23581 gtggtcaagc accacgatgg ctttgtcctt tatccgacag ctcacacaga ttattcggtt
    23641 aaggtcagtc cttggaggag aggaaagggc gacttgctcc ttgaagtatc ccaagctgcc
    23701 acagagtttg atatggatat gggggtctac ctgtcaccgt gggatgccca tagtcccctc
    23761 tatcatgtgg accgagaagc ggactacaat gcctattatc tggctcagtt gaaggaaatc
    23821 ttatcaaatc ctaactatgg gaatgctggt aagttcgctg aggtttggat ggatggtgcc
    23881 agaggagagg gcgcgcaaaa ggttaattat gaatttgaaa aatggtttga aaccattcgt
    23941 gacctgcagg gcgattgctt gattttttca acagaaggca ccagtatccg ctggattggc
    24001 aatgaacgag ggtatgcagg tgatccactg tggcaaaagg tgaatcctga taaactagga
    24061 acagaagcag agctgaacta tcttcagcac ggggatccct cgggcacgat tttttcaatc
    24121 ggagaggcag atgtttccat ccgtccaggc tggttctacc atgaggatca ggatcctaag
    24181 tctctcgagg agttggtcga aatctacttt cactcagtag ggcgaggaac tccactcttg
    24241 cttaatattc cgccgaatca agctgggctc tttgatgcaa aggatattga acgactttat
    24301 gaatttgcga cctatcgcaa tgagctctat aaagaagatt tggctctggg agctgaggta
    24361 tctggtccag ctctttccgc agactttgct tgtcgccatt tgacagacgg ccttgagacc
    24421 agctcttggg caaacgatgc agacttgccc atccagttag aactcgactt aggttctcct
    24481 aaaacttttg atgtaattga gttgagagaa gatttgaagt taggccaacg tatttctgct
    24541 ttccatgtgc aagtagaggt ggatggtgtc tggcaggaat ttggcagggg cttcacagtt
    24601 ggtcataagc gcctcttgcg aggtccgcta gtagaggcac agaaggtgcg cgtgatgatt
    24661 actgaagcac aatctatgcc tctactgacc aagatttccc tttataaaac tcctggatta
    24721 tcaaaaaaag aagttgttca ggaactagca tttgcagaaa aaagcctagc tgtggcaaag
    24781 ggagagactc ttcattttag gattgaacgt agtgaaagta gctcctcttt agaggctaag
    24841 atttcgattc aaccggggac aggtgtccat ggtgtcgcct atcaggatga gattcaagtt
    24901 cttcaattcc aagcagggga aagcaaaaaa gatttgcatc taccaaccct gtatttcgca
    24961 ggagataaaa ccttggattt ctatctgaac ctaacggtgg atggtcagct tgtggatcaa
    25021 cttcaagtcc aagtttcata aaagaagaac ctttgcgcga tgcaaaggtt cttttggtta
    25081 ttaatgactt ggtaaccagc tgagggtgaa agttagttgt tcagctttta agaggtcttg
    25141 gtgttgaata gttgatacga gtgttttgtc cagtcggcat tctttgacaa agttaaaatg
    25201 gttgtggttt tgtttagtat ggatatccag ccatttatct tctttagcga ggtagactcg
    25261 tagatggtca aagagagaga ttccgaggtc atagcttggt tttcctggac aggttggata
    25321 aaatccgaga gctgaccaga tgtaccaagc agagagacta ccattgtctt catctccagg
    25381 ataggcttcc caacttgggt gaaaagcttt ctgacggagc gtcttgataa gaagggcagt
    25441 gtagtcaggg taatcgctgt aacggaagag ataaggaatg tggaaactag gctggttgga
    25501 aatggctatc tgtccaaaag gagcagtagc catctcgctc atttcgtgaa tttcgtaacc
    25561 atagcctgtt gtttcaaaga ggggagcatc ttgacaggct ttcaaaagat agttgctaaa
    25621 ggtttctttt ccacccatca gttggattaa gccagggatg tcgtggagaa cgcctaaagt
    25681 agcttgaatg gcagagcatt cagcgtagtc tcgcccccaa ctataaggag agaagtcagg
    25741 gtgaaagttt ccttgattgt ctcgtgctcg catgtaacct gtctcagcgt caaatagctg
    25801 gcggtaattt tgtgaagcag ccttgtaggt ttcagcgatt tctatgtttt ttagtttttt
    25861 ggcacagttg gcgatacaaa agtcactata ggcatagtct agagtatggc taacactttc
    25921 gtggtggtcg gtagagaggt aacctagttc ttggtattgg gctagtccgt ggcggccatt
    25981 gatgccgaga gggtcggctt tgctggctgt tttgagcatg gcttggaaga gttctccttc
    26041 taggtcgggg gccatgtcct tgcaggcgct atctgcgata ataccgtcta aaagtgtacc
    26101 tggcatcata ccccgttcat ctggagccag ccattttgga aggaaaccag tatcgcggta
    26161 gctattgagg aaaccttcta aaaagcgttg atagtgctcc ggtatgataa gggcaaagag
    26221 ggggaaggtg gtgcggaagg tatcccagaa accattgttg ctaaagagga caccaggctt
    26281 gacagtacca gtagccagat ccatgtggat ggcttgccct gactcatcaa cctcataaaa
    26341 agtctgtggg aagaggaaga gtctgtagag gcagtggtca aagaaggttc ggtcagcctc
    26401 tcctgtctct ataatgtcaa aacgatggag gagattttcc caatccgctt gggcacttgt
    26461 tttacagcta tcaaaatctt cttgaggtag attgattaga gcttgagaag gagagatgaa
    26521 agaagtggct agttgcatct cggtttgact acttgctaag tcaattcgcc agtctccagc
    26581 ttcttggctg atagcaagaa tatccgtgtt catttgcagg gcagtgaaca tcgttagcga
    26641 atttttgtta gtttcagttt taccttcttg ttgcagggca agagttcgct tatctacttg
    26701 ctctactgtc agttcatctg ttgcgtgaag atagagggag agggctttgc cttgcttttg
    26761 cttcaaacga atagaagcac cgtagcaagt cggtgtgagc tgggtttcaa tctgatagcg
    26821 cagggagaaa atctttagat aatgaggttg gaaagaggcc ttatccctat cataggaaga
    26881 ttggcggtga aagaggctgt ctccacccag ttgactggtg acaggtgtca gaaggagcca
    26941 agagtagtcc ccaatccaag gactgggctg gtgagttaat cgaatcccct gaaagatagg
    27001 cagatgtgga tcaaaaaacc aagatccatc ctggtcactg gtctggggca caaagtaatt
    27061 catcccaaaa ggcacgcctg tgtatggcag ggtatttccc cgagaaaagg catgcttgtt
    27121 ggtagttcca aagcgggtat cgatggtttc aagtagtggt ttcatagtct ttcctttagc
    27181 tgtttttcta cattatatca gaaaaagagg gcctttagaa aaggagtttc aaaaataact
    27241 attttgtaga aaaatgacta tcatttgtgt atgtattttc tgtctcaaaa gctatatact
    27301 gaacatgaaa cttgtttgaa agaggtaaaa tttaaaatgg tttattcgaa agaaatcgtc
    27361 agagagtggc tagatgaagt agcagagcgg gctaaggact atccagagtg ggtggatgtt
    27421 ttcgagcgtt gctacaccga taccttggac aatacggttg aaatcttaga agatggttca
    27481 acttttgtct tgactgggga tattcctgcc atgtggcttc gagattcgac agcccaactc
    27541 agaccctacc ttcatgtagc taaaagagat gccctcctgc gtcagaccat tgcaggtttg
    27601 gtcaaacgtc agatgacctt ggtactcaag gatccctatg ctaactcctt caacattgag
    27661 gagaactgga aagggcacca cgagactgac cacacagacc ttaacggctg gatctgggag
    27721 cgcaagtatg aggtggattc gctttgctat cctttgcagt tggcttatct cctctggaaa
    27781 gagactggcg agactagtca gtttgatgag atttttgtcg cagcgactaa ggaaattctc
    27841 catctgtgga cggtggaaca agaccacaag aactctcctt atcgttttgt ccgagatacg
    27901 gaccgtaagg aagacacctt ggtaaatgat ggctttggac ctgactttgc agtgacaggt
    27961 atgacttggt cagcttttcg tccgagtgat gactgttgcc agtatagtta cttgattccg
    28021 tcaaatatgt ttgctgtagt agtcttgggt tatgtgcaag aaatcttcgc agcattaaac
    28081 ctagctgata gccagagtgt tattgctgat gccaagcgtc ttcaggatga aatccaagaa
    28141 ggaatcaaaa actacgctta caccaccaac agcaagggcg aaaagattta cgcttttgaa
    28201 gtggatggcc taggaaatgc cagcatcatg gatgatccaa atgtaccaag tctactagct
    28261 gcgccctatc tgggctactg ttcggtcgat gatgaagtgt atcaagctac tcgtcgtacc
    28321 attttgagct ctgaaaatcc atacttctac caaggagaat acgcaagcgg tctcggcagt
    28381 tctcatacct tctatcgcta tatctggcca atcgcccttt ctatccaagg cttgacaaca
    28441 agagataagg cagagaaaaa attcttgctg gatcagctgg ttgcctgcga tggtggtaca
    28501 ggtgtcatgc acgaaagctt tcatgtagat gatccgaccc tctactctcg tgaatggttc
    28561 tcctgggcta acatgatgtt ctgtgagttg gtcttggatt acttggatat tcgctaaggg
    28621 gctcgcttta gctcaaccga ttcttatcag aatcacaagt ttacatttaa aacgttaaaa
    28681 tttaaattta gaatgaggtt ttacttcatg gaaaatgttg ttgtacatat tatctcacat
    28741 agtcactggg accgtgagtg gtacttgcct tttgaaagtc atcgtatgca gttggtggaa
    28801 ttgtttgaca atctctttga tctctttgaa aatgaccctg agttcaagag tttccacttg
    28861 gatggacaaa ctattgtcct tgacgactac ttacaaattc gccctgaaaa tcgtgacaag
    28921 gtccaacgct acattgacga gggcaagctt aaaattggtc ccttttacat cttgcaggat
    28981 gattatttga tctccagtga agccaatgtc cgcaatacct tgattggtca acaagaagct
    29041 gccaaatggg gtaaatcaac ccagattggc tactttccag atacctttgg aaatatggga
    29101 caagcgcctc aaattcttca aaaatcaggc attcacgtgg cgacctttgg tcgtggtgtg
    29161 aagccgattg gatttgacaa ccaagtcctt gaagatgagc agtttacatc ccagttttca
    29221 gaaatgtact ggcagggtgt ggatggtagt cgtgttttag gtattctctt tgccaactgg
    29281 tacagtaacg ggaatgaaat tccagttgac aaagatgagg ccttgacctt ctggaaacaa
    29341 aaattgtcag atgtgcgtgc ctacgcttcg accaaccaat ggttgatgat gaacggctgt
    29401 gaccaccagc ctgtacagaa aaatctgagc gaagccattc gtgtggcaaa tgaactcttc
    29461 ccggatgtaa tctttgttca tagttccttt gatgaatatg ttcaagctgt agaaggtgcg
    29521 cttcctgaac acttatcaac tgttacaggt gagttgacca gtcaggaaac agatggctgg
    29581 tacacacttg ccaacacttc ttcatcccgc atttacctaa aacaagcctt ccaagaaaat
    29641 agcaacctcc tagagcaagt ggtagaaccc ttgactatta tcactggtgg acacaaccac
    29701 aaggaccagt tgacctatgc ttggaaaaca cttttgcaga atgcgccaca tgatagtatc
    29761 tgtggctgta gcgtggacga agttcaccgc gagatggaaa cgcgttttgc caaggtcaac
    29821 caagtaggaa actttgttaa aagtaatttg ctcaacgagt ggaagggtaa aattgctacg
    29881 gataaggctc aaagtgacta tctctttact gtcattaaca caggcttgca tgataaggtc
    29941 gatactgtca gcacagtgat tgatgtggcg acttgtgatt tcaaggaatt gcacccaaca
    30001 gaaggctaca aaaagatggc tgctcttatc ttgccaagtt accgtgtgga ggacttggat
    30061 ggtcgtcctg tagaggctac aatcgaagac ctcggagcta attttgaata cgatttacca
    30121 aaagacaagt tccgccaagc tcgtattgct cgacaagtgc gcgtgaccat tccagttcac
    30181 ctagcaccgc tttcttggac aaccttccaa ttgctggaag gagaacaaga acaccgtgag
    30241 ggtatttacc aaaacggagt gattgataca ccattcgtaa cggtgagtat ggatgacaac
    30301 atcacagtct atgataagac aactcacgaa gcctatgaag actttatccg ctttgaagac
    30361 cgtggggaca tcggaaacga gtatatctat ttccaaccaa aaggaacaga gccaatcttt
    30421 gcagagctta agggccacga ggtcttggaa aacacagctt gctatgctaa aatcttgctc
    30481 aaacatgaat tgaccgtgcc tgtcagcgcg gatgaaaagc tagaagaaga gcaacaaggt
    30541 atcatcgagt ttatgaagcg tgaggctgga cggtcagaag aattgacaaa cattcctctg
    30601 gaaactgagt tgactgtctt cgttgacaat ccacaaatcc gcttcaagac tcgctttact
    30661 aacactgcca aggatcaccg tatccgtctc ttggtcaaga ctcataacac gcgtccaagt
    30721 aatgattctg aaagtatcta tgaggtggtg acacgaccaa acaaaccagc tgcttcatgg
    30781 gaaaatcctg aaaatcctca acaccaacaa gcttttgtca gtctgtatga cgatgaaaaa
    30841 ggggtgactg tatccaataa gggattgaat gaatacgaaa tccttgggga taacaccatt
    30901 gccgtgacca ttttgcgtgc atcaggtgag ctaggtgact ggggctactt cccaacgcca
    30961 gaagcacaat gcttgcggga gtttgaagtc gaatttgcga ttgaatgcca ccaagcccaa
    31021 gaacgcttct cagcctatcg tcgtgccaaa gccttgcaga caccgtttac cagccttcag
    31081 cttgctagac aggaaggaag cgtggttgcg actggtagcc tcttgagcca ttctgttctc
    31141 agcataccgc aagtttgtcc aacagccttt aaagtggcgg aaaatgaaga aggctatgtc
    31201 ctccgttact ataatatgag tcaagaaaat gtgcgcatat cagaacatca acaaaccatt
    31261 cttgacttac ttgagcgacc atatccagtt cattcaggac tattggctcc acaagagatt
    31321 cgtacagaat tcatcaaaaa agaagaaatt taatttcaaa aagtaaacat caaaagaaag
    31381 gaggggcgaa aaggtaagaa ctaactactg attcgcccct ttttatggta aaaacaatga
    31441 ccattgcaac gattgatatc ggagggactg ggattaagtt tgccagtctg actcctgatg
    31501 ggaaaatact ggataagaca agtatttcaa cgcctgaaaa cttggaggat ttactaacgt
    31561 ggctagatca acgcttgtca gaacaggatt acagtgggat tgctatgagc gttccaggtg
    31621 cggtcaatca agagacaggt gtgattgatg gcttcagtgc ggtgccctat atccatggct
    31681 tttcttggta tgaggcgctt agctcttatc agctacctgt ccatttagaa aatgatgcca
    31741 actgcgttgg actcagtgaa ctactagctc atccagagct tgaaaatgca gcctgtgtcg
    31801 tgattgggac agggattggc ggagccatga ttatcaatgg tagacttcat cgaggtcgcc
    31861 acggtctggg tggagaattt ggctacatga caacccttgc ccctgctgaa aaacttaata
    31921 actggtcgca actagcatca actgggaata tggtacgata cgtgattgaa aaatctggtc
    31981 atactgattg ggacggtcgc aagatttacc aagaggccgc agctggtaat gctctttgtc
    32041 aagaagccat tgagcgcatg aaccgcaatc tgacgcaagg cttgctcaat atccagtatc
    32101 tcatcgatcc agatgtcatc agtctgggtg gctctatcag tcaaaatcca gattttatcc
    32161 aaggtgttaa gaaggctgtt gaagactttg tcgatgccta cgaagaatac acggtcgcac
    32221 cagttatcca ggcctgcacc tatcacgcag atgccaatct ctacggtgcc cttgtcaact
    32281 ggctacagga ggaaaagcaa tggtaagatt tacaggactt agtcccaaac aaacgcaagc
    32341 tattgaggtt ttaaaaggtc acatttctct accagatgtg gaagtggctg tcactcagtc
    32401 tgaccaagcc tctatctcta tccagggtga ggaaggtcac tatcaattga cctaccgcaa
    32461 acctcaccaa ctttatcgtg ccttgtcctt gttggtaaca gttctagcag aagctgataa
    32521 agtagagatt gaggaacaag cagcttacga agatttggct tacatggttg actgttctcg
    32581 aaatgcggtg ctgaatgtgg cttctgccaa gcagatgatt gaggtcttgg ctctcatggg
    32641 ctactcaacc tttgagcttt acatggaaga cacttaccag attgaaggac agccttactt
    32701 tggctatttc cgtggagctt attcagcaga ggagttgcag gaaatagaag cctatgctca
    32761 acagtttgac atgacctttg taccatgcat ccagaccttg gcccacttgt cggcctttgt
    32821 caaatggggt gtcaaggaag tgcaggagct ccgtgatgta gagaacattc ttctcattgg
    32881 cgaagaaaag gtttatgacc tgattgatgg catgtttgcc acgttgtcta aactgaagac
    32941 tcgcaaggtc aatatcggga tggacgaagc ccacttggtt ggtttgggac gctacctgat
    33001 tttgaacggt gttgtggatc gtagtctcct catgtgccaa cacttggagc gcgtgctgga
    33061 tattgctgac aaatatggtt tccactgcca gatgtggagt gatatgttct tcaaactcat
    33121 gtcagcggat ggccagtacg atcgtgatgt ggaaattcca gaggaaactc gtgtctacct
    33181 agaccgtctc aaagaccgtg tgactctggt ttactgggat tattatcagg atagcgagga
    33241 aaaatacaac cgtaatttcc gcaatcatca caagattagc catgaccttg catttgcagg
    33301 gggagcttgg aagtggattg gctttacacc tcacaaccat tttagccgtc tagtggctat
    33361 cgaggctaat aaagcctgcc gtgccaatca gattaaagaa gtcatcgtaa cgggttgggg
    33421 agacaatggt ggtgaaactg cccagttctc tatcctacca agcttgcaaa tctgggcaga
    33481 actcagctat cgcaatgacc tagatggttt gtctgcgcac ttcaagacca atactggtct
    33541 aacggttgag gattttatgc agattgacct tgccaacctc ttaccagacc taccaggcaa
    33601 tctcagcggt atcaatccca accgctatgt tttttatcag gatattcttt gtccgattct
    33661 tgatcaacac atgacacctg aacaggacaa accgcacttc gctcaggctg ctgagacgct
    33721 tgctaacatt aaagaaaaag ctggaaacta tgcctatctc tttgaaactc aggcccagtt
    33781 gaatgctatt ttaagtagca aagtagatgt gggacgacgc attcgtcagg cctaccaagc
    33841 ggatgataaa gaaagtttac aacaaatcgc cagacaagaa ttaccagaac ttagaagcca
    33901 aattgaagac ttccatgccc tctttagtca ccaatggctg aaagaaaaca aggtctttgg
    33961 tttggataca gttgacatcc gtatgggcgg actcttgcaa cgcatcaaac gagcagaaag
    34021 ccgtatcgag ttttatctgg ctggtcagct tgaccgcatc gacgagctgg aagttgaaat
    34081 cctaccattt actgacttct acgcagacaa ggatttcgca gcaactacag ccaaccagtg
    34141 gcataccatt gcgacagcgt cgacgattta tacgacttaa tactcttcga aaatctcttc
    34201 aaaccacgtc agcttccatc tacaacctca aaacagtgtt ttgagcagcc tgcagctagc
    34261 ttcctagttt gccctttgat tttcattgag tataaaaaca agaacacctt gcttggcgca
    34321 gggtgtttcg cgtgaaacag aagaattatc tggtttcaaa tgctacagtt agacaaactt
    34381 atgataaaat agcagaaagt gaatgtttcc taagagcaat tctatcgatg agcaattgga
    34441 ggtattatgc tacacttaaa attagtaaaa caagaaatag aagctgaaaa gccagcatct
    34501 gtagaagctt ggatcatttc cgtcaaattt taaaaaggtt gctaccgaca tatatagatt
    34561 ccaaaaacaa aaacgttagc ggaactagca gatgtgattt tatggagttt tgattttgca
    34621 aatgatcatg ctcacgcatt tttcatggat aatgttgagt ggagtcatgc agattcttac
    34681 tttcttagct ttgttagtga cgatgttgaa gaacgttaca cagaaaatgt ctatctggat
    34741 agcctaagtg tcaaacaaaa atttaagttt attttcgact tcggtgatga atggcgtttt
    34801 gaatgccaag tgctgagaga aatcgagaca gaggacgaag aagcttatct cgtacgttcg
    34861 gttggaacgt cgctagaaca atatccagat tatgatggtt ttgactatga agaatggtaa
    34921 aattgaaatc taaaaaacaa ttggaatcag tctgtgtagg cttagtattt caatagactt
    34981 cctgcaaaac tagaatccta gttcatgatt gataatacca gcactcaaat tcattcgtaa
    35041 tccaaagcgt ttacgatgat ttcgataggt tgttgaaaac attttaaacg tttttacttt
    35101 ggcaaggatg ttctcaacct tgcttctatc cttagatagt gcatggttat aggttttatc
    35161 ttcaactgtt agtggcttga gtttgctgga tttacgtgga atttgtgctt gaggatatat
    35221 cttcatgagc ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct
    35281 gcgactcatt ttgaacaact tcatatcatg acaatagttc acagtgatat ccaaagaaac
    35341 aattctccct tgacttgtga caatcgcttg agccttcata gcgtggcatt tctttttacc
    35401 agaatcattc gctaattctt tttttagggc gattgatttg tacttccgtc gcatcaatca
    35461 ttaccgtgtc ctcagagctg agaggagttc ttgaaatcgt aacaccactt tgaacaagag
    35521 ttacttcaac ccattggctc cgacggatta agttgctttc gtgaatacca agatcagccg
    35581 caatttcttc ataagttcga tattctcgca catattgaag agtggccata agaaggtctt
    35641 ctaggcttaa tttaggtttt cgtccacctt ttgcgtgttt aagttgataa gctgttttta
    35701 atacagctaa catctcttca aaagtggtac gctgaacacc aacaaggagc ttaaatcgtg
    35761 catcagttag ttgtttactt gcttcataat tttgtttcgc agggagtcta ttgactcttt
    35821 ggtaggtgtc aatgtttttt tcatctatcc cgagaattat tttcccgcca tttgtatttg
    35881 caaatgctga gtaggtttcc cagaaagact ctggaagatt gtttttagct tttttgtatt
    35941 ctaaatcaac cccttcaaat tttaagtcca tatttttcct ttacatctgt tttttgtggt
    36001 tctggtattt gttcaagttg agtgataata tagcgaattg aatttcgaga gtttttactc
    36061 agttaatttc ttttttaacc cactttaatt tcttttttaa cacgggttaa aaaagaaatt
    36121 aaagtgggtt aatttttctt gacttaaaat ttaaaaatga tatactgtgt ttatggagag
    36181 atgacaattt ataaaatgta ctactctatt ctactagata atgattgaat aaaacttttt
    36241 ataacaacgg ctagcaaagt tataatttta tatctattag aaaataaata tgtaaggaaa
    36301 tttaaatgaa aaaaactaca atattatcat taactacagc tgcggttatt ttagcagcat
    36361 atgtccctaa tgaaccaatc ctagcggata ctcctagttc ggaagtaatc aaagagacta
    36421 aagttggaag tattattcaa caaaataata tcaaatataa ggttctaact gtagaaggta
    36481 acataggaac tgttcaagtg ggtaatggag ttactcctgt agagtttgaa gctggtcaag
    36541 atggaaaacc attcacgatt cctacaaaaa tcacagtagg tgataaagta tttaccgtta
    36601 ctgaagtagc tagtcaagct tttagttatt atccagatga aacaggtaga attgtctact
    36661 atcctagctc tattactatc ccatcaagca taaaaaaaat acaaaaaaaa ggcttccatg
    36721 gaagtaaagc taaaactatt atttttgaca aaggcagtca gctggagaaa attgaagata
    36781 gagcttttga tttttctgaa ttagaagaga ttgaattgcc tgcatctcta gaatatattg
    36841 gaacaagtgc attttctttt agtcaaaaat tgaaaaagct aaccttttcc tcaagttcaa
    36901 aattagaatt aatatcacat gaggcttttg ctaatttatc aaatttagag aaactaacat
    36961 taccaaaatc ggttaaaaca ttaggaagta atctatttag actcactact agcttaaaac
    37021 atgttgatgt tgaagaagga aatgaatcgt ttgcctcagt tgatggtgtt ttgttttcaa
    37081 aagataaaac tcaattaatt tattatccaa gtcaaaaaaa tgacgaaagt tataaaacgc
    37141 ctaaggagac aaaagaactt gcatcatatt cgtttaataa aaattcttac ttgaaaaaac
    37201 tcgaattgaa tgaaggttta gaaaaaatcg gtacttttgc atttgcggat gcgattaaac
    37261 ttgaagaaat tagcttacca aatagtttag aaac
//
LOCUS       DAYYQN010000010        36919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYYQN010000010 DAYYQN010000000
VERSION     DAYYQN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.010"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(628..1476)
                     /locus_tag="ACHBRQ_000490"
     CDS             complement(628..1476)
                     /locus_tag="ACHBRQ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HFM9592212.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1628..1906
                     /locus_tag="ACHBRQ_000491"
     CDS             1628..1906
                     /locus_tag="ACHBRQ_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HFM9592213.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1908..3971
                     /locus_tag="ACHBRQ_000492"
     CDS             1908..3971
                     /locus_tag="ACHBRQ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9592214.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(4049..5305)
                     /gene="tyrS"
                     /locus_tag="ACHBRQ_000493"
     CDS             complement(4049..5305)
                     /gene="tyrS"
                     /locus_tag="ACHBRQ_000493"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9592215.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5447..7912
                     /gene="pbp1b"
                     /locus_tag="ACHBRQ_000494"
     CDS             5447..7912
                     /gene="pbp1b"
                     /locus_tag="ACHBRQ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HFM9592216.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7977..8888
                     /locus_tag="ACHBRQ_000495"
     CDS             7977..8888
                     /locus_tag="ACHBRQ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFM9592217.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            9118..9816
                     /gene="dapD"
                     /locus_tag="ACHBRQ_000496"
     CDS             9118..9816
                     /gene="dapD"
                     /locus_tag="ACHBRQ_000496"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9592218.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9884..11014
                     /locus_tag="ACHBRQ_000497"
     CDS             9884..11014
                     /locus_tag="ACHBRQ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFM9592219.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            11026..11565
                     /locus_tag="ACHBRQ_000498"
     CDS             11026..11565
                     /locus_tag="ACHBRQ_000498"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9592220.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11549..12226
                     /locus_tag="ACHBRQ_000499"
     CDS             11549..12226
                     /locus_tag="ACHBRQ_000499"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9592221.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12293..13192)
                     /gene="galU"
                     /locus_tag="ACHBRQ_000500"
     CDS             complement(12293..13192)
                     /gene="galU"
                     /locus_tag="ACHBRQ_000500"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9592222.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(13214..14230)
                     /locus_tag="ACHBRQ_000501"
     CDS             complement(13214..14230)
                     /locus_tag="ACHBRQ_000501"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9592223.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14388..14882
                     /locus_tag="ACHBRQ_000502"
     CDS             14388..14882
                     /locus_tag="ACHBRQ_000502"
                     /inference="COORDINATES: protein motif:HMM:NF037495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592224.1"
                     /translation="MIQYMLIIEVNNSGGSCRLREFGEKIKRLRLAKKISRSEFCGDE
                     SELSIRQLIRIENGESRPILTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRT
                     PTYEDETIAQKKESVFDKIFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEI
                     LSFW"
     gene            complement(14997..15428)
                     /locus_tag="ACHBRQ_000503"
                     /pseudo
     CDS             complement(14997..15428)
                     /locus_tag="ACHBRQ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15490..16140)
                     /gene="phoU"
                     /locus_tag="ACHBRQ_000504"
     CDS             complement(15490..16140)
                     /gene="phoU"
                     /locus_tag="ACHBRQ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9592225.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(16155..16907)
                     /gene="pstB"
                     /locus_tag="ACHBRQ_000505"
     CDS             complement(16155..16907)
                     /gene="pstB"
                     /locus_tag="ACHBRQ_000505"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9592226.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16909..17724)
                     /gene="pstA"
                     /locus_tag="ACHBRQ_000506"
     CDS             complement(16909..17724)
                     /gene="pstA"
                     /locus_tag="ACHBRQ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9592227.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17717..18580)
                     /gene="pstC"
                     /locus_tag="ACHBRQ_000507"
     CDS             complement(17717..18580)
                     /gene="pstC"
                     /locus_tag="ACHBRQ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9592228.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18698..19573)
                     /locus_tag="ACHBRQ_000508"
     CDS             complement(18698..19573)
                     /locus_tag="ACHBRQ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9592229.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19740..21071)
                     /gene="pnpS"
                     /locus_tag="ACHBRQ_000509"
     CDS             complement(19740..21071)
                     /gene="pnpS"
                     /locus_tag="ACHBRQ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HFM9592230.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(21072..21779)
                     /locus_tag="ACHBRQ_000510"
     CDS             complement(21072..21779)
                     /locus_tag="ACHBRQ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9592231.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21863..22349)
                     /locus_tag="ACHBRQ_000511"
                     /pseudo
     CDS             complement(21863..22349)
                     /locus_tag="ACHBRQ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            22335..22894
                     /locus_tag="ACHBRQ_000512"
                     /pseudo
     CDS             22335..22894
                     /locus_tag="ACHBRQ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23209..24900)
                     /gene="argS"
                     /locus_tag="ACHBRQ_000513"
     CDS             complement(23209..24900)
                     /gene="argS"
                     /locus_tag="ACHBRQ_000513"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9592232.1"
                     /translation="MNTKELIASKLSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTIAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            25036..25482
                     /gene="argR"
                     /locus_tag="ACHBRQ_000514"
     CDS             25036..25482
                     /gene="argR"
                     /locus_tag="ACHBRQ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9592233.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            25533..28067
                     /gene="mutS"
                     /locus_tag="ACHBRQ_000515"
     CDS             25533..28067
                     /gene="mutS"
                     /locus_tag="ACHBRQ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9592234.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            28214..29908
                     /gene="patA"
                     /locus_tag="ACHBRQ_000516"
     CDS             28214..29908
                     /gene="patA"
                     /locus_tag="ACHBRQ_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HFM9592235.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(30058..30501)
                     /locus_tag="ACHBRQ_000517"
                     /pseudo
     CDS             complement(30058..30501)
                     /locus_tag="ACHBRQ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30699..32465
                     /gene="patB"
                     /locus_tag="ACHBRQ_000518"
     CDS             30699..32465
                     /gene="patB"
                     /locus_tag="ACHBRQ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HFM9592236.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            32604..33293
                     /locus_tag="ACHBRQ_000519"
     CDS             32604..33293
                     /locus_tag="ACHBRQ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9592237.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            33297..33476
                     /locus_tag="ACHBRQ_000520"
     CDS             33297..33476
                     /locus_tag="ACHBRQ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592238.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            33463..34812
                     /locus_tag="ACHBRQ_000521"
     CDS             33463..34812
                     /locus_tag="ACHBRQ_000521"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9592239.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            34936..36396
                     /gene="gltX"
                     /locus_tag="ACHBRQ_000522"
     CDS             34936..36396
                     /gene="gltX"
                     /locus_tag="ACHBRQ_000522"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9592240.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            36410..36841
                     /locus_tag="ACHBRQ_000523"
     CDS             36410..36841
                     /locus_tag="ACHBRQ_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9592241.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT        10770 a         7053 c         8121 g        10975 t
ORIGIN      
        1 gtttactctc aatcatcaag aattctcttt tcgtggtagc gtttggggtc tggtactggc
       61 cttatatcac ttattgttca ttgataagtt tgtaatttcg aatcgaaaat aaagattaga
      121 actatgattg actggctact tttaatattg attttggagg aagattttgt ctctattatt
      181 tattatttta aatttattta ttttgtataa gatctattct ttgaggcggg aaaaatcgaa
      241 atacttgatt tatacggcct atatcatatt tggggtaaat gtagtatatg gtattcaatg
      301 gttattaaga gaactgattt caactatttc cccttaatgt gtatggaaac tataacaaaa
      361 cgcataagat taaggtttgt gtacctgttc ttatgcgttt ttataatatt gaagattttc
      421 ggtaagtttt ctacttttgc gaagtctagt ttatactcaa tgaaaatcaa agagtaaact
      481 agccgtaggt tgctcaaagt acagctttga ggttgcagat aaaactgacg aagtcagctc
      541 aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agtaaccata cgtacggcaa
      601 ggcgacgttg acgtggtttg aagagtatta gaatgctttc ccaaccaaaa tctctgcttc
      661 gatggtaatc tctgtcagtt ggctccagtc aatcttgctc tggtcaacgt gaaagaggag
      721 aggagtcata ctgagtaggg cttggagttg ttctgctgtg atagtcttag tcagagaggc
      781 agtttgacta gataggatgg taaagtgttc ctggaaatgc tccttgatat cttggttaga
      841 atactccttg tttgtcagct ggtcctgtac tctttgacgg atttctttga ggtgattttc
      901 agttgggata acctttatca agataccgtc tttggataaa acgcgacgaa attctccata
      961 gttggcaggt gagaagatat caagcagaat atccatgtta gcgtctttta taggaaggcg
     1021 tgccaagtcg ccaacgaacc aattgactgc ccagttgggt tcacttttag cagcgatttg
     1081 gactgaatct ttggagatat caaaggcata gaaagttttt tcagagtgac tttcttgtag
     1141 tttgcgagaa tagaatcctt caccacaacc gatatccaaa attgttgtgg tagtttttga
     1201 gcttgcaagc aagtcagata cagcatctaa gatagcttgg taaaagccgg cttctaggat
     1261 ttgttgacgg ttttgaaaat tttccttgtc atagttagca gattgcttga tttgaggtgc
     1321 tagattgaca tagccaaatt tcgccaagtc aaaagaatga cggttgcagc acttgaaatt
     1381 agtctctaac agagtcagat tttcttgaca gataggacag gcaaaggcag tcgcagaagc
     1441 aaaacgctga agtttgggct tgagatttgt attcatagtt taagtgtatc aaaagaggca
     1501 aatgaaagca actttaggaa taagtagatg ggagattcag tagaaataaa actaattctc
     1561 caatttatag aatgaaattg cttttatatc taaattgcta tataataatg ataaggagga
     1621 aataagtatg ttaaaagaag tattaaccgt tgcaaaagtt gcgaaaaaat cttcattatt
     1681 tttgggtggt gtcgcatttg gtacccttgg tttgaaaatc ttagcaagta aggaagctaa
     1741 aaaaggttat tctaaagctt tggctaaggc ttacaagttg aaagacgagc tagatgcatc
     1801 tgtttctgtt gtgaagcaac atggagacga tgtcttgcaa gatgccaaat atttgtacga
     1861 gcaagagaaa aaagaagagc aattagatag ccttataggt gaataatatg tcttttaaag
     1921 tgctacatag aggataccaa catatccgac tatcatcttc tttttcactt accttggata
     1981 ttcaagacta tcttcgttcc ttggcgagag atgaaaaggg gattgagtct atccagtttt
     2041 acatggatca acagcacttt actctacgca taaaagaagg cttttctgta ttagataatg
     2101 cagaagcctt tttaaaaaga attgataaag ggaaagtttc tgagttgatg actcttccca
     2161 ttcgtagaga agagagtgct tattctattg tttcaggtgc agcggttaag cgtgtacttt
     2221 ttcgtagttt tgtgccgtat cctattcgct atatatggac ttgttatcag gctttgggtt
     2281 atattagaga agcctatcaa acactagcgc gtaaggaact aactatggaa gtcttggact
     2341 gttcggctat tttattgtct ttgtttatga accaatccaa gacagctagc aatatcatgt
     2401 ttatgcttga tttggggaat catttagatc agtggtcctt gaaaaaaact gcaacagatt
     2461 tagaacagag tcttcttgca aaagagagcg atgtattcct agtacagggc gatacggttg
     2521 ttagtataaa gagttccgat gttcaaatag gagatgtctt gatcttatct caaggaaatg
     2581 aaattctgtt tgatgggcaa gtagtttcag gtttaggtat ggtcaacgaa agttccttga
     2641 caggagagag ttttccagtt gaaaaaagag agtctgattt ggtttgtgca aatacagtat
     2701 tagaaactgg agagttacgc attcgtgtaa cagataatca gatgaacagc cgtattttac
     2761 agctgattga gttgatgaag aaatctgaag aaaacaagaa aacgaaacaa cgctatttca
     2821 tcaagatggc ggataaagtc gtcaaatata atttcttggg ggctgggctg acttacctat
     2881 tgacaggttc tttttctaag gctatttctt tcctattggt cgatttctcc tgcgctttga
     2941 aaatctctac tcctgtagct tatttgacag ttatcaagga ggggttgaac cgtgaaatgg
     3001 tgattaagga tggagatgtt ctggagaaat atctggaagt tgatactttc ttgtttgata
     3061 agacaggaac aatcacaact agttatccta tagttgaaaa ggtgttacct tttggagact
     3121 atagtgagga agatattctc agaatcagtg cctgtcttga ggaacacatt tatcatccta
     3181 ttgctaatgc catcgtcaag caagctgaga tagagggaat tgaacatgag gaaatgcatg
     3241 ggaaactcca atatatcgca agcaagggga tcaaatctca tatagatggc caaccagttc
     3301 ttattgggaa ttatgtcttg atgcaggatg agcagattca tatcagttca gaacaaaatg
     3361 ctttaattga agagtacaag agtcactaca atctcttatt cttggcttat caaaatgaat
     3421 tgattggaat gttctgcatt catactcctt tgagaaaaga agcaaaaaca gccttggata
     3481 aacttaaggc acaggggaaa aaattgattc tggcaacagg agacaccttg attaggacag
     3541 aggaattagt caaagatttg ccctttgatc aggtctatac agacttgaaa cctgatggaa
     3601 aatttgagtt agtagagaaa ctgcagaaag caggtcacac tattttgatg gttggagatg
     3661 gattgaatga ctcagcggct ctaactctat cagatatcgg tgtggtgatg aatgagagtg
     3721 cagatatttc taagcagatg agtgatatct tattgttaga taatcgtttg gatttcttcc
     3781 aagagttgga ttcgttatca tcatctttgc aaacactcat caagaaaaat attcaagata
     3841 ccgttgtcgt aaatagtagt ttgattggct tcggcttatt taattggctc agtccttcaa
     3901 atctctctat cttacataat ctaacaacct tgcgcattgt cctgcgtagc ctgtctatta
     3961 aaaatagata gatgagagtt attaacagca aaaaggagtt acctttctcg aggttaactc
     4021 ctttgtttat agataaatgt tgaatagttt agtaagtcaa tacaaagtat tttttcttcc
     4081 cacgacggat aacagtcagt tcattttcta acttatcagc gtcactcaag acatagtcaa
     4141 gctcttggat gcggtcgccg tttacgtaga tggctccgtt ttggacgtct tcacgggctt
     4201 ggcgttttga gttaactata ccagatgaga cgagcagttc cacgatattg ttgttttcgt
     4261 ctgcctgtac ttggtagttt ggcacaccac gaagtccttg tttgagctct ttgacagaaa
     4321 ggtttttgat gtttcctgca aagagttgct cagtgatgtt aagtgcttct ttgtaggctt
     4381 cttctccgtg aacaagtgta acaacttcac gagccaagac tttttgagcc aagcgttcgt
     4441 gtggcgctgc ttcaaattgt ttacgaatat cttcaatctc atcaagtgac aagaaagtaa
     4501 agattttcaa gaagcgaaca gcgtcagcgt ccatcacgtt catccagaat tggtacattt
     4561 cgtatggaga agtcttttcg ggattgagcc agacggcatt tccttctgat ttaccaaatt
     4621 tcttaccagt tgcatctgtg attagtggaa cagtgataac gtgaccagtc ttgtccgcct
     4681 tacgacgaag caattcggta ccagctgtca tatttcccca ctggtcagaa ccaccgattt
     4741 gaagagtgac attatggtct tggttaagga cgaagaagtc atacccttgc atgatttggt
     4801 aagcgaactc agtgtaagaa attcctgttt cgatccgttt tttaacagat tccttactca
     4861 tcatgtagtt gaccgtgaag tattttccaa tatcacggag gaagtcaatg aagctgatgc
     4921 tgccaaacca gtcgtagttg ttgaccatga cagccttgtt ttcgccattt tcaaagtcaa
     4981 gaaaacgaga aagttgtcct tggatagact tgacccagcc atctactgtg tcttttgttt
     5041 ggagactacg ttcagcatct ttgaaggacg gatctccgat gagacctgta gcaccgccaa
     5101 cgagcgcata aggtttgtga cctgctagtt gcaagcgacg acttgtcaag attgcgacaa
     5161 ggtggcctag gtgaaggctg tcagcagttg gatcgtagcc agtataataa gaaacttgac
     5221 cttcttctag ggctttacgc aaagcttctt catcagtcgt ttgaaatatc aaaccacgct
     5281 cttttagctc atcaaaaatg tgcatgtgtt ttttctcctt tttaaaatta ttgtttctac
     5341 ctattgtatc acaaacttgg gcaatagcct agcagaatag ggaagaaagt ggctatttta
     5401 ttgaaaattt cccttttgtg gtataataga taaagtgagg aaatccatgc aaaatcaatt
     5461 aaatgaatta aaacgaaaaa tgctggaatt tttccagcaa aaacaaaaaa ataaaaaatc
     5521 agctagacct ggcaagaaag gttcaagtac caaaaaatct aaaaccttag ataagtcagc
     5581 cattttccca gctattttac tgagtataaa agccttattt aacttactct ttgtactcgg
     5641 ttttctagga ggaatgttgg gagctgggat tgctttgggg tacggagtgg ccttatttga
     5701 caaggttcgg gtgcctcaga cagaagaatt ggtgaatcag gtcaaggaca tctcttctat
     5761 ttcagagatt acctattcgg acgggacggt gattgcttcc atagagagtg atttgttgcg
     5821 cacttctatc tcatctgagc aaatttcgga aaatctgaag aaggctatca ttgcgacaga
     5881 agatgaacac tttaaagaac ataagggtgt agtacccaag gcggtgattc gtgcgacctt
     5941 ggggaaattt gtaggtttgg gttcctctag tgggggttca accttgaccc agcaactaat
     6001 taaacagcag gtggttgggg atgcgccgac cttggctcgt aaggcggcag agattgtgga
     6061 tgctcttgcc ttggaacgcg ccatgaataa agatgagatt ttaacgacct atctcaatgt
     6121 ggctcccttt ggccgaaata ataagggaca gaatattgca ggggctcggc aagcagctga
     6181 gggaattttc ggtgtagatg ccagtcagtt gactgttcct caagcagcat ttttagcagg
     6241 acttccacag agtcccatta cttactctcc ttatgaaaat actggggagt tgaagagtga
     6301 tgaagaccta gaaattggct taagacgggc taaggcagtt ctttacagta tgtatcgtac
     6361 aggtgcatta agcaaagacg agtattctca gtacaaggat tatgacctta aacaggactt
     6421 tttaccatcg ggcacggtta caggaatttc acgagactat ttatacttta caactttggc
     6481 agaagctcaa gaacgtatgt atgactatct agctcagaga gacaatgtct ccgctaagga
     6541 gttgaaaaat gaggcaactc agaagtttta tcgagatttg gcagccaagg aaattgaaaa
     6601 tggtggttat aagattacta ctaccataga tcagaaaatt cattctgcca tgcaaagtgc
     6661 ggttgctgat tatggctatc ttttagacga tggaacaggt cgtgtagaag tagggaatgt
     6721 cttgatggac aaccaaacag gtgctattct aggctttgta ggtggtcgta attatcaaga
     6781 aaatcaaaat aatcatgcct ttgataccaa acgttcgcca gcttctacta ccaagccctt
     6841 gctggcctac ggtattgcta ttgaccaggg cttgatggga agtgaaacga ttctatctaa
     6901 ctatccaaca aactttgcta atggcaatcc gattatgtat gctaatagca agggaacagg
     6961 aatgatgacc ttgggagaag ctctgaacta ctcatggaat atccctgctt actggaccta
     7021 tcgtatgctc cgtgaaaatg gtgttgatgt caagggttat atggaaaaga tgggttacga
     7081 gattcctgag tacggtattg agagcttgcc aatgggtggt ggtattgaag tcacagttgc
     7141 ccagcatacc aatggctatc agaccttagc taataatgga gtttatcatc agaagcatgt
     7201 gatttcaaag attgaagcag cagatggtag agtggtgtat gagtatcagg ataaaccggt
     7261 tcaagtctat tcaaaagcta ctgcgacgat tatgcaggga ttgctacgag aagttctatc
     7321 ctctcgtgtg acaacaacct tcaagtctaa cctgacttct ttaaatccta ctctggctaa
     7381 tgcagattgg attgggaaga ctggtacaac caaccaagac gaaaatatgt ggctcatgct
     7441 ttcgacacct agattaaccc taggtggctg gattgggcat gatgataatc attcattgtc
     7501 acgtagagca ggttattcta ataactctaa ttacatggct catctggtaa atgcgattca
     7561 gcaagcttcc ccaagcattt gggggaacga gcgctttgct ttagatccta gtgtagtgaa
     7621 atcggaagtc ttgaaatcaa caggtcaaaa accagggaag gtttctgttg aaggaaaaga
     7681 ggtagaggtc acaggttcga ctgttaccag ctattgggct aataagtcag gagcgccagc
     7741 gacaagttat cgctttgcta ttggcggaag tgatgcggat tatcagaatg cttggtctag
     7801 tattgtgggg agtctaccaa ctccatccag ctccagcagt tcaagtagta gttctagcga
     7861 tagcagtaac tcaagtacta cacgaccttc ttcttcaagg gcgagacgat aattctctaa
     7921 atgaagtggc caatcaatgg attgccactt ttttcttttc gtgttacaat aagggtatga
     7981 atcagtatca gaaaaagatt gttaacggaa aaatttattc gctcctatcc ggcttaatat
     8041 ggggaatctg tggaatttta ggagagtact tctttactca ttatcaggtg tcttcgggct
     8101 ggattacctc tatgcgtttg acactggcag ggagtcttgt actcatttgg tctgcaatac
     8161 aattaaaatc gcaagtgcta gatatttggc gagacaagaa aaattacctg ccctttttag
     8221 cctatgctat tttggggatt ttttcagttc agtatttttt ctatctctgt gtagaatact
     8281 caaatgctac gacagcaact attttacagt ttattagccc tgtctttatc ctcttttaca
     8341 atcgcttggt ttatcaaaaa cgagcgtcaa aaagcgctgt tttctatgtt ttggttgcca
     8401 tgctgggtgt ttgcttgatg gcgacaaagg gagatctctc tcagttatcc atgacgccgc
     8461 tagctcttat aacaggtttg ctgagtgcca tgggtgttat gtttaatgtt atcttgcccc
     8521 aaccttttgc taagcgttat ggttttgttc ctacggttgg gtgggggatg attttggcag
     8581 gtttgtttag caatgtcctc tcgccggttt atcagctttc ctttactctt gatatttgga
     8641 gtatcttgat ttgcctcatt atcgctttct ttggaacggc ttttgctttt ttcatttcca
     8701 tgaaggctgt gtccttggtt tctcctttgg tggttgccgt tatcagtgcc agtgaacctc
     8761 tctcttctgc tctcttgagt gttttgttct taggattagt agtggattgg tccctccttc
     8821 tagctatagc cttgattatt ttacccatga tatttttgtc tatagaagaa gcgaaagaaa
     8881 gtagataaaa agtcttttct taattctaaa agtgtgctat actagaatag tcaataaaac
     8941 acggggagat agttgtgaag agtgttttta ggttgtatcg gtaggggctt gcttttaccg
     9001 acagcacgtt ttatctcaca accctaaaaa atcataccta aaaacagaca aaaaggtttc
     9061 aaatttgagg ccttttttgg tagaataggt atcattataa cgaaccagga ggcacctatg
     9121 actgctacaa aaatgaacgc tcaagaaatt atccaattta tcgccaatgc tgaaaagaaa
     9181 accagtgtca aagtaacctt tgaggggcaa ctcgcaactg ctgtgcctag ctctgttgtc
     9241 aaactaggaa atgtcctatt cggagactgg aaggacgtgg ctccgcttct tgaaggtttg
     9301 gtagaaaatc aagattatgt tgtcgagcaa gatgctcgta attctgcagt tcctttgcta
     9361 gataagcgtg ctatcaacgc tcgtatcgag ccaggtgcta ttatccgtga ccaggtggaa
     9421 attggtgaca atgctgttat catgatggga gctgttatca atatcggtgc tgaaatcggt
     9481 gctggaacca tgattgacat gggtgccatc cttggtggcc gtgccatcgt tggaaaaaat
     9541 agccacgttg gtgcaggtgc agttttggca ggtgtgattg agccagctag tgctgaacca
     9601 gtccgtgtcg gagacaatgt tcttatcggt gctaatgcag tggttatcga aggagtccaa
     9661 atcggtagtg gttcagttgt cgctgcagga gctatcgtta cccaagatgt cccagaaaac
     9721 gtggtagtag caggtgttcc agctcgtatc atcaaagaaa ttgatgccca aacccaacaa
     9781 aaaacagcgc tagaggatgc gcttcgtacc ttgtaattgt aaaagtaaaa aagaggcgga
     9841 accctttttc cagcctcttt ctgctatatc ggaggacaga tagatgttag atttgattca
     9901 gactagacga gctttacacc agattccaga gattggcttg gaggagttca agactcaggc
     9961 ttatttgctg gatgtgattg agaaattgac tacgggcaag gattttgttc aaattcgtac
    10021 ttggcggaca gggattttgg tctacctgca gggaagtcag ccggagcgaa ccattggttg
    10081 gcggacagat attgatggtc tgcctatcgt cgaacaaaca ggtctacctt ttgcttctca
    10141 acaccaaggt cgcatgcatg cctgtggtca tgattttcat atgaccattg ccttaggctg
    10201 tcttgagcgc gcccttgagg agcaacccaa gaataatctg ctctttctat ttcagcctgc
    10261 tgaagaaaat gaagctggtg gtatgctcat gtatgaggac ggtgcttttg gagattggtt
    10321 gccagatcaa ttctatggtc tccatgttcg tccagatttg aaagtcggcc agattgcgac
    10381 taatactcat acactctttg caggaacttg cgaggtgaag atccgtttca aaggcaaagg
    10441 tggccacgca gcttttcctc atgaagccaa tgacgccttg gtggcggcta gttactttgt
    10501 gacccaagtg cagtcagttg ttagccgcaa tgtcaaccca atcgagggag cagtggtgac
    10561 cttcggcgtt tttcaagctg gaaccaccaa caatgtcatc acagacacag cttttttaca
    10621 tggaactatt cgtgccttga cacaggacat gagtctcttg gtgcaaaaga gggtaaaaac
    10681 agttgcagaa ggggttgcag ctgcctttga tatggaagtc gaagtggaac tcaaacaagg
    10741 tggataccta cctgttgaga acaatccagc cttggcgcgt gaactgatgg acttctttga
    10801 cgaaaaagac ggaatcgagt tgattgatat cgaacctgct atgacaggcg aggactttgg
    10861 ttatctcctt tcaaaggttg atggcgtgat gttttggcta ggtatcgata gtccctacgc
    10921 tcttcatcac cctcaaatga gtcctaagga agaagtctta gctattgggg tggctgcggt
    10981 gtctagtttc ctgaaaaaga aggcagcaga atagagggct tgtctatgaa atcggaatta
    11041 cgcaagcaag tcttgcatga aatgaaggct ttatctcaag aacaaaaaca ggctatagac
    11101 caagctttaa ccgagcgaat tttacaacac cccttttatc aagaagccaa ggtcatcgca
    11161 acctacctct ctttttctca tgagtttcaa acgcgggaac tgattgagca ggcgctgaag
    11221 gacggcaaga aggttttgac acctaagacc tatcccaagg ggcgcatgga ctttgtagtc
    11281 tatgatccgc agcagttggt aaaaactgcc tttgccttac tggagccaca gggagatttg
    11341 gaagtggtgg atgtatctca gattgatttg attcatgttc ctggcctggc ttttacgacg
    11401 gatggatatc ggattggata tggtggaggt tattatgacc gctatctgga acatttttct
    11461 ggtcatactt tgagtacggt tcatccttgt caaattcagg actttatacc tgaaaaccat
    11521 gatattcctg ttcaggaggt attaattgat gaaggaaatc tttgatagac gttaccctgt
    11581 gacgagtttc ttcctcttag tgacggcctt ggtattttta ctaatgttgg tcactgcagg
    11641 cggaaacttt gacagggcag atacattatt tcgatttgga gccatgtatg ggccagctat
    11701 tcgcctcttt cccgagcagg tttggcgtct cttgtctgcc atttttgttc atattgggtg
    11761 ggaacatttc attgttaata tgctttcact ttattatctt ggaaggcagg tagaggagat
    11821 tttcggctct aagcagttct tttttctcta tctcttatca ggaatgatgg gcaatctctt
    11881 tgtttttgta tttagtccta aatccttagc agcaggcgcc tctacctctc tttatgggct
    11941 atttgccgcg attattgttc ttcgctatgc aactcgcaat ccttatatcc aacagctagg
    12001 gcaatcctat ctgacacttt ttgtggttaa cattattgga agtgttctga ttccaggaat
    12061 cagcctagca ggccatatcg gtggtgcagt tggtggcgca tttctagcag ttatctttcc
    12121 agttagagga gaaaaacgga tgtataacac cagccagaga ttaggagcgg tagtcttgtt
    12181 cgtaggactc gccattttgc ttttctacaa gggaatggga atgtgacgaa gttgaaagaa
    12241 aatttgtgta atgaaaaaag ataaggcatt ttttgaacct tatcttttta ttttattcct
    12301 tctcagtcaa ttcttttcca agttggatga ggtaattctt caaatcatct ttgacttgtg
    12361 gatgtttgag ggcgtagtca atagatgttt tcataaagcc aaacttatcc ccaacgtcgt
    12421 aacgagctcc tgtaaattca cgggcaaaaa cacgctgtgt tttattaaga gtatcgattg
    12481 catcagtaag ttggatttca tttccagcac caggagcttg gttttcgagg atttggaaaa
    12541 tttcaggcgt gaggaggtag cgtccgataa tagcaaggtc gctaggagcg tcctctggag
    12601 ctggtttttc aacaaaggtt tcaacactgt aaagaccatc ttttccttcg ccttgcggag
    12661 caataacccc gtaagcagat acttcgtcat gagggactgg catgacagcg atagtagacg
    12721 cgtgggtacg ctcgtagtca tccatgagtt gtttggtaag tggaacagcc ttttcgtctg
    12781 tgatatccat caagtcatca ccaagcataa cgacaaaagg ttcatttccg acgaaagcct
    12841 tggcttgcaa aacagcatct ccgagaccgc gtggatgagt ttggcggata aaatgcagcc
    12901 gcatgtcagt tgttttatca actagcttca aaagatctgt tttccctttt tctttgaggt
    12961 tatattccaa ttcgaagttt gaatcaaagt ggtcctcaat agaacgtttt gatttaccag
    13021 tgacaactag aatatcttca atacctgatt tgagagcttc ttccacgata aactggatag
    13081 ttggtttgtc tacgattggc aacatttctt tggcaagggc cttggttgct ggtaaaaatc
    13141 gagttcctag tccagcagca gggatgactg cctttctaac ttttgatgtc ataaaaatcc
    13201 tttctataga gggttaagac cactcatttt ctgctttaaa ttcattgttc atgatgtcat
    13261 aaatggcatc tttgatattg gttccgtgat aaataacttg gtaaatagcc tgtgtaatgg
    13321 gcatatagac tccaagttct tgggctagtt cataggctgc tcgagtcgtt gaaattcctt
    13381 cgattaccat gcccatatta gcttctatat cagctaggga ttctcctcgt ccgagagcat
    13441 ctccagctct ccagttacga gagtggatgg aagttcccgt tacgatcaaa tctcccacac
    13501 cagataagcc gctataggtc aatggactgg ccccgagtgc tacccctagg cgggtgattt
    13561 ctgctaaacc tcgagcgatg atggctgcct tagcattatc accaaatcca agaccatgta
    13621 aagctccagc accgacagca ataatatttt taagagcacc agcagtttca accccgataa
    13681 catccgtatt ggtataaagt cggaagtagt gattactaaa tagcttctga acgtattgag
    13741 ctgtttgtaa atctttagaa gcagcagtta ttaaagttag gtcacgcaca atggtctctt
    13801 ctgcatgact aggccctgaa acaacgacga tatcactacg gagatgttca ggaatttctt
    13861 cttcaagaat ggttgataat cgtttatggc tatcaggttc taatcccttt gatgcgtgca
    13921 tgatgataac cttatggtcc aaggtttgtg caacttgctg ggcaacaagt cgtgtcactt
    13981 ttgttgggac aacaaacaaa atcgcatcca catctttcaa tgtttctgct aagtcggtgt
    14041 aggcaatgat attttcgtct agaacgacat ctttaaagta gtgcttatta gtatggtgtg
    14101 tattaatttc attgatttgc tcgggaagat ttccccaaat acgtacctcg tgtccattgt
    14161 catttaagac ttgtgaaagg gcggttcccc aagaaccagg ccccaagacg gcgacggttt
    14221 gtttttccat gatttcctcc ttgatagagt tctttctctt attctatcat attctagggg
    14281 attttgcact tgcattactg gggtatggtg gaaaaataca caaaaaccga gacgaaggga
    14341 taaactctta gtctttagtt aaagtgagaa gaaatctgat agatagtttg attcagtata
    14401 tgcttataat agaggtaaac aactcaggag gttcttgtag gttgcgagag tttggcgaaa
    14461 aaattaaaag attacgtttg gctaaaaaaa tcagtcgttc agaattttgt ggtgatgagt
    14521 ctgaattaag tatccgtcaa ttaattagaa ttgaaaatgg agaatccaga ccaatactaa
    14581 caaagttaaa atatattgct gaacgtttgg aggttgaaga ttacaagttg atgccaagtt
    14641 atatagagtt ggataaggaa tacctagaat tgaagtattt cttgatgagg actcctacat
    14701 acgaagatga aactatcgcc caaaagaaag agagtgtttt tgataagatt tttgaagagt
    14761 attatgatag gctacctgag gaagaaagat ttatcatccc aaattattca tatctggcac
    14821 taacgaacta cacagttcaa aaattaccag aaaagctagt tgaaatactg tccttctggt
    14881 gattttgctt aatttctgat gttcgagaca tgattgagga gatttgtctt gattttttcc
    14941 taaaaaaggg cagactcctc ccttggtttg atgctcgatt tttaaaataa ggtgacttat
    15001 tctgttaggc atgttttccc ctgccctcta gctctatatg gaacagggag tatcagattt
    15061 atctggcaga ttgtatcaaa taaggcttga aactgttttg aataggcata tagacttgag
    15121 aatttgagaa tatgtcgtct agcagtagag acatattttc cggcaatatg aaggaagaga
    15181 cgtcgaaaac gcttgatagt caaggccttt acttcatcac cagctagctg ctttaaaaag
    15241 aggtagagat tataagccag acagcccatc atcatacgta cttcattctt aatcatggtc
    15301 gaactatctg tcttatcccc aaagaatcct gctttccttt ctttgataaa gttttcagca
    15361 ttccctcgtt cacgatagaa ttgaaacagc tcctcactat ctacactgct taaattggtc
    15421 aaatgaactt ttttacaacc ataaaaaaga gcacttaatc gtactcttct cttttaagga
    15481 ttagttgaat taattcaaat ccactagttc tcctgtttct aggtagacta ggcgttcaca
    15541 aatgttagca atgtaatcag cgaagcgttc cagatgccct atgatataaa ggtattgagt
    15601 tccattggga attgaggttt cttggtcttt cataagtcca atgatttcct ttgataaggc
    15661 ataataatat tggtcaatct gttcatcttt ttgagcaata ctaatagctt ttgaggcttg
    15721 gtgcaaagga aaggcaacca ataaatcggc tagcatgctg agggataatt tacccatttg
    15781 gtgtaactgt tcttcgtcag gggctagttg attttctttt agttgcaaaa cagctttggc
    15841 aatgcctgcc atatggtctc ccatacgttc aaggtctgaa caagaagaca tgatgctaat
    15901 cacaaatcga aggtcagaca cttgtggctg ctgcaaggcc aacaaacggg cacaggtcaa
    15961 ttcgatagcg ctttgacctt ggttgatagc atgatcctta ttgataatta gctctgccat
    16021 ctccttgtct ttggaggcta aggccagtaa ggcttttgaa gctgtttcaa ggacaagttg
    16081 ccctagtcct aaaaaggatt gttctaattc atgcaattct aagtcaaatt gatttctcat
    16141 aggtttttcc ttccttatcc gaaccgtcct gaaatatagt cttctgtgcg ctgatctttt
    16201 ggattggtaa acacgtcaac ggtatctcca aattcgcaaa tttctcctgt taagaaaaaa
    16261 gcagttttat ctgaaatacg tgaagcttgt tgcatgttat gggtaacaat gataatcgta
    16321 taatcctttt ttagttgctg aatgaggtct tcaattttta aagtggagat agggtctaag
    16381 gctgaagtcg gctcatccat taacagaata tcaggttcta ctgctaaagc tcgcgcaatg
    16441 caaaggcgtt gctgctgacc gccagataag gacatggcac tctttttaag atcatctttg
    16501 acttcttccc aaatggctgc cccttttaaa gatttctcca ctaaggcatc taattgtttt
    16561 ttgtctcgaa taccatgtgt ccttgggcca taagccacgt tatcatagat agacatggca
    16621 aagggattag gctgttgaaa aaccatccct acacgcttac gtagctgatt aaggttgaat
    16681 ttgctactat aaatatcttg ctcatctaag aggacttggc cttcaatatg gcaagaagga
    16741 accaaatcgt tcatccggtt aagggttttt agaaaagttg atttgccaca accagatggg
    16801 cctatcaagg cagtaatctg tctttctggt aattgaatcg aaatattttt taaggcttga
    16861 aaatccccgt aaaataagtc taggtgtctg actgaaaatg ttcccatact aggaagctcc
    16921 tttcacaagt tttcgagata ataagcttga tagagtattt atcattaaaa cagtaataat
    16981 caaaatcaca ccggtagcat aggcttcatt gacatgtagc ccctcacttg acagcatata
    17041 catatgcagg gctagagaac gacctgaaga catgagacta cttggcgtat tggtagaggt
    17101 acctaatgta tacatgaggg cagctgtctc accaacgata cggccaatag ctagtatcac
    17161 tccagctaaa atacctggca tggcaactgg tagaacaatt ctaaaaacag tccgtaactt
    17221 acctgcccca agtccataac ttgcttgacg catgctatca ctaacagata aaagggcttc
    17281 ttctgttgag cgaataatga ctggcaacac catgatgact gaggttaaga ttcctgataa
    17341 cagagagtat tgaaaaccta agaagactac aaagaagagc atgccaaaca gaccaaaaac
    17401 aatggaagga atcccagaca aggtatctga ggccaatcgc atgattttaa cacaaaggga
    17461 atcttttttt gtatattcca caagataaaa accagcaaaa atccctatgg gcaaggctaa
    17521 aagaagagca ccaaagacca gaataacggt ggaaataatc gctggcataa gggaaatgtt
    17581 ctcagaagta taagtccaag aaaagaggga tagacttaga tgaggtaagc ctttgatgag
    17641 gataaaacca atgattaaaa agagagagcc aaaggttaaa gctgaaaaac aataaacgag
    17701 aagttttagc aggtatttac tcataagatg attttccttt caagtaggca aagtaggcat
    17761 taatcaagag aataaggaaa aagagaactg ctgaggttgc aataagggct tccctatgct
    17821 gacctgatgc gtaagccatt tccagaacaa tattggttgt taaggttctg gttcctgaaa
    17881 agagtccact tggaataatc ggctggttgc ctgccaccaa aataactgcc atggtttcac
    17941 ctactgcgcg accgattcct aaaataactg ctgataaaat accagatctc gcagctggca
    18001 agatgacact aaaaatactc cgttcatgac tagctcctag agccaagcta ccagaataat
    18061 acgttttggg aactgttcgg atagcagatt ctgacaaact gataatggtt ggcaaaatca
    18121 ttattcctaa tagtaacgaa gcggttagga cactcatgcc atttcctaaa aagcttctaa
    18181 tccaaggcac caataattgt aggccgaaaa aaccataaac aatagatgga atggccgcca
    18241 tcaagttgat agctgatttt aagaagccat agacgggctt tggacaataa taaaccataa
    18301 acaccgatgt caagatgcct gttggcaccc caatcacaat cgctcctaag gtaattaata
    18361 aggaaccaac gatcattggt aaaataccat agcttgccgg aatgttcgtt ggcgaccaat
    18421 cactgcctaa taaaaaacgg gcaaagccgt agttagccat gaaaggtaag ccattactaa
    18481 aaataaagaa acagattagc aaaatagcta caacagctac tgttgcactc atgaaaaaaa
    18541 ttgccctaaa aactgcttct ttgaaggctt gttttgtcac atcttgtcct ttctagtgaa
    18601 gaaagtaagg gagatacgac acctccctac ttgccttctt tatcttattg tacgatgaaa
    18661 cgtctgcatc tctttagaga tttatggagc aaacatttta tttaatcttg tcccaggtgg
    18721 ttaatttgcc actaaaaacg tctgcaagtt cagccatact gacttggctt gccttattgt
    18781 cattattgac cacaacagca ataccgtcta aagcaatagc atcatgggtg agactcttac
    18841 cttcttcagg agttaattcc ctagaaacca taccaatatc agcggttttc tccttaacag
    18901 cggtaatacc tgctgaagac ccattagagg taatatcaat cgtaacttct ggattttctt
    18961 ttttataagc ttctgctaat ttttccatta aagaagatac tgaagtggaa cctacaacag
    19021 acaacttgcc tgataagtgt tggcttgtat attctgtggt ttcagtttta gcttcaataa
    19081 atttattatc tgtgaccact tgttgacctt gtttggagtg gataaagctg ataaaatctt
    19141 gacctagctt ggaaagatta gaagaccaaa caatgttgaa gggacgttga agagggtatt
    19201 caccatctaa aactgtgtct cgactagcct tgacaccatc aatctctaaa gctttgacag
    19261 atttcgttaa agatcccaag gagatgtagc cgatagcatt agcattccct tgaactgctg
    19321 agagaacacc ttctgtacta ttttgaatca cagctgtttt ggcagtgttg tcaatttttt
    19381 tatcaccgtc ttttttgaga atccctgtga tttctgtgaa ggcaccccgt gttccagagc
    19441 cattttctcg tgaaatcacc tcaatcgttc ctgaagctga ctgtttggaa gcagctgact
    19501 gattgccaca ggcaacaagc ccaaatcctg ataagccaat ggctgcaaga gtaagcattt
    19561 ttttgaattt cataataatc acctttatct ctatgtattt ttcttgtgta ggcttactac
    19621 atttatagtc taacaagtct ttgtaaaggt ttatccctga ttcatgtaaa gattgtgtaa
    19681 agaatcaaaa aaagccactt ttgaaaaatg gctgctccta aaaatagctt taaaaattat
    19741 tagtcctgtg cgaaagattg gttaggaaga aaaatcgtga agcaactgcc tctgccaagc
    19801 tgactcgtca ccgtgacttg gccacctaat aattgactga gttctttgac aatggcaagg
    19861 ccaagaccag tgccaccagt ttgtctgctt cgacctttat taactcggta aaaacgttca
    19921 aaaatacgat cctgctctaa ttgactaata ccaatccctg tatctgatac agaaatctta
    19981 atgccttcgt tcaccttttg ggtcttgacc tcaatttttc ccccttgttc agtgtaacgg
    20041 atggcattgg ataaaagatt gagtaagatt tgggaaagta attgactatc tgatacgagg
    20101 gtgacatcat ctggcacctg cacctttagc tgtaaatcct tcttcttgag ctgaggttgc
    20161 aagctttgag tcaaatcctg tacaaattct gccaaagaaa gggtcgtcca ttgtataggc
    20221 atttgttgag ccttagataa ggtaagaaga tgctcaacaa tatgctcaag acgcaagctt
    20281 tctttgtaaa taatgtctag aaagtcatcc ttgagcgctt cttcttcagc tgacatcccc
    20341 ttaatggttt cagcaaagcc cttaatcgaa gtaactggtg tcctcaattc atgggaggca
    20401 tttgagacaa aggctaaatt taacttttca taagttctaa tcgttgttaa atcatatagc
    20461 aagacgagca cagcttccac agattgggtg gggctaaaaa cgggaactgc tgtcacttct
    20521 aaaatcaagt caccctcatg aaacccactt acttcttgtt ttaaccttgt tttttgatca
    20581 aaggcttggt gaactaaatt tcgaatatcc atccgtttga ggtcatcaag tgaacttatg
    20641 tcgccgtcca catcgggaaa ataatgaggc agagagcgac tggataataa catctgacct
    20701 tgagcggaaa ctaaaaacgt ccccatggtt aggtgcgaca gaagaacctc cattgtttcg
    20761 gctaggtcct tgtattgctg atcctgttgg gagactttgg tttttagggc agacacatac
    20821 tgagccaaag actttaagtc ttcttgccct ttttctaaaa agtattcact actggtcaag
    20881 agaggttggt gcaaggtctc aaaagcaact tcccatttcc aaaggcaaaa gagccagtag
    20941 ccaccttgtc ccaaagaaag ggctagaaga aagagaccga tgcctttact gatccaagtt
    21001 aatgccatcc ctgcaatcag aatgaggcta acacttagat tgactagcca aaattgaagg
    21061 tagcgtttca tctataactc cttgaactta taaccataac cccgaatggt tcgaataaat
    21121 tgaggggctt taggattgtc ttcaattttt tccctcaact taccaatatg aacgtccacc
    21181 aaacgtgttt cctgcccaaa gtcatacccc cagatacgtt ccaaaagacg ctctctagtc
    21241 agtgtcatgt tgggatgttt cataagatag agcaagagtt caaattcttt tggggtcaaa
    21301 ctcagtaact tattcgcctt gtagacttca tgacgctcag ggtatacttt caaggtccca
    21361 aatagccaag aatcgtcagc gatattatct gaatcatctc cttcttgttc tcctttagtt
    21421 cgcctgagga cagccttgac acgcgccagc aattctctag ggctaaaagg tttggtcagg
    21481 tagtcatcag cccctaattc caaggccaaa accttatcaa attcatcact tttcgcagaa
    21541 accatcataa ttggagtttt gacgcctttg gctctcagcc gcttacaaac ttccatgcca
    21601 tctaattgtg gtaacatgat atcaagcaag ataaaatcaa agggttctgt ttctgccaaa
    21661 gctaaggcct tccgtccatt tgttaccaat tgagtagaaa agccttcctt acttaaatgg
    21721 tagtcaagca atttcagaat gtgttcttca tcatccacta ataagacttg ttttgtcatc
    21781 tattatctcc tattggtaac attataacac aattatcaga aatcctaaca ttgctaaatc
    21841 agattaaatt tgcttatcaa gactagtatc tggtcaaacg ctcaatcatc tccttgtgct
    21901 ctggataggt cgccagtaga tctacccttt caaataattc aaaatcctca aattcaaaac
    21961 caggagcaac aagacaagaa accagagcat catccttatc aactgttgat ccccaaatag
    22021 tgcccttagg aacacagtag tgaagttgtt gccctttgga tatgtccagg cctaaagtga
    22081 ctgcttcgta gtgaccatct gctgtaatca tgtgaacagt aagtggggat tctgcatgaa
    22141 aataccagat ttcatctgct gtcaatcggt gaaaatgtga aggattcgtt tcttcttaat
    22201 aagaaataaa tactggtata aagcgccctt cccttaccag caaggtttat agtgtctgaa
    22261 gctttttttg tttgtctaaa atagccacct tcaacatggg gagctaactc tagagttctt
    22321 atcaagtctt ctttatccgt cggagccaat gggttgaagt aactcttgtt caaaggtggt
    22381 gttacgattt caagaactcc tctcagctct gaggacacga taatgattga tgcgacggaa
    22441 gtaaaaatca atcgccctaa aaaaacaatt agcgaataat tctggtaaaa agaaatttca
    22501 cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcgc
    22561 tgtgaactat tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc
    22621 tggtaaaatc ttggctgaca gtggttatca agggcccatg aagatatatc ctcaagcaca
    22681 aactccacgt aaatccagca aactcaagcc gctaacagtt gaagataaag cctgtaacca
    22741 tgcgctatct aaggagagaa gcaaggttga gaatattttt gccaaagtaa aaacgtttaa
    22801 aatgttttca acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat
    22861 tgctggcatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt
    22921 aggtgaatta gtgaagcgtt taggcaagtg tctctggtta cgacgtcatg gcctctaaat
    22981 cgattatatt taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta
    23041 gcgtctaaca attaggaact ttagttccaa taactttaag attacgacgt tttaggacat
    23101 aaatcgatca tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat
    23161 ttggcgttag ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc
    23221 tggcgcttct actccaagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct
    23281 gagggctaga cggctgtcgc gttctgggct ttcatccaag atacgtgtat gtgcatagta
    23341 tttgttaaag gattgagcca ggctaattgc aaatttagca atgatagaag gttcaaagtt
    23401 atctgccgca cggttgataa tacgtgggaa gtcttgaatg agtttaatga tttcccagct
    23461 ttcagtatca ttcaagctat agttgccagc tgtttctggt ttgaaatcgg ctttgcgtaa
    23521 gatagattgg atacgagcgt aggcatattg aacgtaaggt ccagtttcac cctcgaagga
    23581 taccatagct tctaggtcga agtcgtatcc atttgtacgg tcggttttga ggtcatagaa
    23641 tttaatggct ccaaccccaa cagcatgtgc tacttggtct ttgttttcta gttcaggatt
    23701 tttagcctcg atttggacct tggcacggct aacagcctct gcaatagtag gctctagcaa
    23761 gatgacattc cctttacgag tagagagctt cttcccttct tttgtaacca gaccaaaagg
    23821 aacgtgggta atgtcgtcac tccaatcgta gcccatctct tgcaagacag ctttgagctg
    23881 tttaaagtgg gcagattgtt cttgaccaac gacgtagata gatttagcaa attggtattc
    23941 atttttacgg tagagggctg cagccaagtc acgtgtgata tagagagttg caccatcaga
    24001 cttcttgatg agggctggat gttcaattcc gtatttctca agattcacaa cttgggcacc
    24061 ttctgattca agaagtagtc ctttttcaga aagaatgtct acaactgcat ccatcttatc
    24121 attgtagaag gcttctccgt tatagctgtc aaattcaacc ttcaattcat tgtaaaggcg
    24181 gttaaattcc actaaacttt catcgcggaa ccattgccaa agagcgagag cttcctcatc
    24241 tccattttca agtttacgga accattcgcg cgcttcttca tccaagctag ggtcattttc
    24301 agcttcagcg ttgatgcgga catagagttt aaggagttca tcgattggat gagcttttac
    24361 agcttcttcg tcgccccatt ttttgtaggc aacaatcaac atcccaaatt gtttacccca
    24421 gtctcccaaa tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa
    24481 gctatctccg ataacagttg aacgcaggtg gccaatagaa aatggtttag cgatattcgg
    24541 actagacatg tcgataacaa cattttcttg tttaccaata ttttggtcag catagtgttc
    24601 tttttcagtg gtaacagctt gcaatacttg agcagaaatg gcagatttat caaggaaaaa
    24661 gttaacgtaa ggtcctgttg cgacaacttt ttcaaaggct tggctgttca ttttttcagc
    24721 cagttcagcc gcaatcattt gtggtgcttt acgttcgact tttgcaagag aaaaagcagg
    24781 gaaagcaatg tctcccattt ctgagttttt aggggtttcc agtaacttta aaatagcctc
    24841 ttggtccagg ctatcaatga tgctagataa tttgctagca atcaattctt ttgtattcat
    24901 taagagctcc tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa
    24961 atttttgaaa tctcctgttt ttttggtata atatggttat aaatatagtt ataaatatgc
    25021 acgcaagagg attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact
    25081 gaggagaaat taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt
    25141 gtgacgcaga caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa
    25201 aatgatatgg tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt
    25261 ttgtctcatc atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa
    25321 ttgggagaag cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta
    25381 ggaacagttg ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa
    25441 ctcatggaag atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct
    25501 caagacttat ttttgaaaag gagagacaga aaatggcgat agaaaagcta tcacccggca
    25561 tgcaacagta tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg
    25621 gtgattttta tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt
    25681 ccttaacgag tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc
    25741 attctgccca acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag
    25801 agcagatgga agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca
    25861 ttacgccagg gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt
    25921 ccatagaccg cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact
    25981 tttatgtgac aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg
    26041 ctcgagaagt ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc
    26101 agatgaatct ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt
    26161 tgcgattggc aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga
    26221 ctcagatgag ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct
    26281 tgcagatgga ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta
    26341 agaaacaagg cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc
    26401 tcttgcgttc ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag
    26461 aagtagtgca ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca
    26521 agggtgttta tgacattgag cgcttggcta gtcgtgtttc ttttggtaaa accaatccaa
    26581 aggatctctt gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag
    26641 aagggatgga gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt
    26701 tggagagttt gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg
    26761 gaattatccg gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag
    26821 ggactagctg gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc
    26881 tcaagattga ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactgg
    26941 gaaatgtgcc agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa
    27001 ccgaagaatt agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc
    27061 tcgaatacga aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac
    27121 aagctctagc ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg
    27181 aaacccagca tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag
    27241 ggcgccatgc tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc
    27301 agatggcaga agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta
    27361 cctatatgcg tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg
    27421 ctgaaagcgc ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg
    27481 acttggtttc gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt
    27541 cgcatgcgac caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt
    27601 atgacgggat ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta
    27661 agaccctctt tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact
    27721 tggtcaatgt ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga
    27781 ttgaaccggg accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc
    27841 cagcagacct tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag
    27901 agagtcctcc tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata
    27961 gggcagaaga gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac
    28021 ctatgcaggt tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac
    28081 tagttaatct agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat
    28141 aacatcacac aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt
    28201 ttaaggagaa agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccctggcttc
    28261 gctcctgatg acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga
    28321 agtcttaggc gccctccttg ctgggaaata tgaagctatt tatagtatcg gggcttggtt
    28381 gattggtgtg gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta
    28441 tattgcccaa ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt
    28501 ttcttatgct gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga
    28561 tatcaaccag attcagaacg ttgtcatgat gaccttccag attcttttca gacttcccct
    28621 cttgttcatc ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat
    28681 tgttctcatg gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc
    28741 tcgttttgcc aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt
    28801 acgtggcgtt cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac
    28861 agaggtctca gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt
    28921 ggaacccttt atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc
    28981 gggaatggtt cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct
    29041 aagccagatt atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc
    29101 catgatttcc atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa
    29161 ggatatccca gatgaagagt tggttggaag ccttagcttt gaaaatgtga cctttaccta
    29221 tccaatggac aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat
    29281 ggttggtgta gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg
    29341 tctctttgat ccacaggaag gggctatcaa aatcggaggt aaggatattc gagaagtgag
    29401 tgaaggaacc ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg
    29461 aacgattgca gataacttga gacaggggaa gggaaatgct actctatttg aaatggagcg
    29521 cgcagccaat attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag
    29581 tccagttgaa gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc
    29641 gcgtgggatt gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga
    29701 tgccaaatca gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac
    29761 cattattatt gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa
    29821 tcaaggacga ttgattggtc aaggcacgca tgcagacttg gttgccaaca atgccgttta
    29881 ccgtgaaatc tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta
    29941 tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc acggcaaaaa
    30001 gctattgaaa aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattacatca
    30061 ctttttgttc gtcaaatggc agctcttttt taggatataa aacagggttc ggataagttt
    30121 ttttgcaagg tggatgatgg ctacattgta atgttttcct tgttctaact tagtcttaag
    30181 ataggcctta aaagcaggtg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta
    30241 ccgcagatga ggggaacccc gtttgaccat tcttcctgct aaatcaatct gacctgactg
    30301 ataaatagaa gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg
    30361 aatatttcga atctcggcta aaatgaccgc ccctaaacga ttctcaatcc cagtaaccgt
    30421 cgtgatgacc gagttgaact cagccatcaa gtcattgaca cattttttcg ccttgtcaat
    30481 gagcctcttg taatgtttga tgttttcatt acaagagata aaacgtttat gcgttatcaa
    30541 actcattacc aattgaaaca aaaagctgtg gttagatcct ttcggaaatt gtcaagcgat
    30601 tggaggaaat gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag
    30661 tagctaactg cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt
    30721 ggcattattt taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg
    30781 cgacttttgc ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca
    30841 atttagttca agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa
    30901 tcatggtcaa tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt
    30961 gtctcatgac gcgcgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta
    31021 agcttgctca gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc
    31081 attttaccag tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca
    31141 tgagcaatat tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc
    31201 tggctctcat caccattgcc agcaccccat tggctttcct tatgctgatt ttcatcgtga
    31261 aaatggcacg taaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata
    31321 tggatgagag catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga
    31381 tggcaggatt tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt
    31441 tctcaggaat tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca
    31501 tctttgctgg ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag
    31561 gtttgattgt tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg
    31621 cagcgagttg gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt
    31681 ttgatgcaga ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa
    31741 gtgttgaaat cagtcatatc gatttttcat acttgcctga taaacctatt ttgaaagatg
    31801 tcagcatttc tgcccctaaa ggccagatga cagcagttgt tgggcctaca ggttcaggaa
    31861 aaacgactat tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt
    31921 ttgatggtaa agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg
    31981 tattgcaaga ttcggtcttg tttagcggaa cgattcgaga caatatccga tttggtgtgc
    32041 cagatgctaa tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata
    32101 tcgaaagttt gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa
    32161 cagggcagaa gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca
    32221 ttctcgatga agcaacttca aacgtagata cggtgacgga aagcaagatt cagcatgcca
    32281 tggaggtggt tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc
    32341 tcaatgcaga tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc
    32401 atgaactttt gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg
    32461 aataagaaag aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca
    32521 cagccttaaa aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt
    32581 tagtattcga aaggagaggc atcatggcta gaacggttgt aggagttgct gcaaatctat
    32641 gtcccgtaga cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga
    32701 tcattcgtca agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac
    32761 gtgattatgt ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc
    32821 agttttatgg agagaaaaag accgtcgaaa gcgatgatta caatctggtc cgtgacgaat
    32881 ttgaattggc actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc
    32941 gcggtgtcca acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc
    33001 actggcaagg actacctttc gggacatctc actctattga gacagtagaa ggaagcgtgg
    33061 tggctaagct atttgggaaa gaaagtcagg tcaactcagt ccatcgtcaa agcattaaag
    33121 atttggcacc taatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga
    33181 ttgagtctat cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta
    33241 atgaagaaga tggcaattta gaattatttg agtatttatt gaatgaactg taacgattgg
    33301 aacatctggt cgttctttct tttatttttt caaaattttt agaatgtggc attttacgca
    33361 aacgtttgaa ttctgataaa aattggagaa attcgacaaa aaaacttgaa aaaaacgaag
    33421 gtaagcgtta tgatagaaaa gaagaaatat tggaggaaga acatgtcaca tattaaattt
    33481 gattattcaa aagttttaga caaatttgtt gcaccacatg aagtggaata catgcaatca
    33541 caagtaacag cagcagatga attgatccgt aaaggaactg gtgctggtag cgacttcttg
    33601 ggatggttgg accttcctga aaaatacgac cgcgaagaat ttgaccgcat cttgaaagct
    33661 gctgagcaaa tcaagtcaga cagcgatgtt ttggttgtga tcggtattgg tggatcttac
    33721 cttggtgcaa aagcagcaat cgacttcttg aatcaccatt ttgctaactt gcaaacaaaa
    33781 gaagaacgca aagctccaca aatcctttac gcaggaaact caatctcatc tacttacctt
    33841 gctgacttgg tagagtatgt tgcagacaaa gacttctcag taaacgtaat ttctaaatca
    33901 ggtacaacaa ctgaaccagc gattgctttc cgtgtcttta aagaactctt ggttaagaaa
    33961 tacggtcaag aagaagctaa caaacgtatc tatgcaacaa ctgaccgcca aaagggtgct
    34021 gttaaggttg aagcagacgc taacggttgg ggaacatttg ttgttccaga tgatatcggt
    34081 ggacgcttct cagtattgac agccgttggt ttgcttccaa tcgcagcatc aggagctgac
    34141 atcaaagctc ttatggaagg tgcgaatgca gctcgcaaag actacacttc agacaaaatc
    34201 tctgaaaacg aagcttacca atacgcagtc gttcgtaaca tcctttatcg taaaggctat
    34261 gcaactgaga tcttggtaaa ctatgagcca tcacttcaat acttctcaga atggtggaaa
    34321 caattggctg gtgaatcaga aggaaaagac caaaaaggta tctacccaac ttcagccaac
    34381 ttctcaactg acttgcactc acttggtcaa tttatccaag aaggaactcg tatcatgttt
    34441 gaaacagttg tccgtgttga caaacctcgt aaaaacgtgc ttattcctac tttggaagaa
    34501 gaccttgacg gacttggtta ccttcaagga aaagacgttg actttgtaaa caaaaaagca
    34561 actgacggtg ttcttcttgc ccacacagat ggtgatgtac caaacatgta tgtgactctt
    34621 ccagagcaag acgctttcac tcttggttac actatctact tcttcgaatt ggcaattgcc
    34681 ctttcaggtt acttgaatgc tatcaaccca tttgaccaac caggtgttga agcttataaa
    34741 cgtaacatgt ttgcccttct tggaaaacca ggatttgaag aattgagcaa agaacttaac
    34801 gcacgtctat aatagaagaa aagagtggtt tgcccactct ttttactctc tttatccata
    34861 gaaattggac tcagccaaga cttgtgatat aatatagaaa gcaaaaaggc agacgcctag
    34921 ataataggag aaactatgtc aaaagatatc cgcgtacgtt acgcaccaag tccaacagga
    34981 ctactacaca tcggaaatgc ccgtacagca ttgtttaact acttgtatgc gcgccatcat
    35041 ggtggaacat ttctcatccg tatcgaagat actgaccgta aacgccatgt cgaggatggt
    35101 gaacgttcac aacttgaaaa ccttcgctgg ttaggcatgg attgggatga aagtccagaa
    35161 tcacatgaga attatcgcca gtctgagcgt ttggacttgt atcaaaaata cattgaccaa
    35221 ctattagctg aaggaaaagc ctataaatct tacgttacag aagaagagtt ggcagctgaa
    35281 cgtgaacgtc aagaagtagc tggcgaaaca ccacgctaca tcaatgaata ccttggtatg
    35341 agtgaagaag aaaaagcggc ttatatcgca gaacgtgaag cagctggtat cataccaaca
    35401 gttcgtttgg ctgtcaatga gtcaggtatc tacaagtggc atgatatggt caaaggtgat
    35461 atcgaatttg aaggtggcaa tatcggcggt gactgggtta tccaaaagaa agacggttac
    35521 ccaacttaca actttgccgt tgttatcgat gaccacgata tgcaaatctc tcatgttatc
    35581 cgtggagatg accatattgc taatacacca aaacagctta tggtctatga agctcttggt
    35641 tgggaagctc cagagtttgg tcacatgacc ttgattatca actctgagac tggtaagaag
    35701 ttatctaaac gtgatactaa tacacttcaa tttatcgaag attaccgaaa gaaaggttac
    35761 cttccagaag cagtctttaa ctttattgct cttcttggtt ggaacccagg tggtgaagat
    35821 gagattttct ctcgtgaaga actcattaaa ctttttgatg aaaaccgcct cagcaagtca
    35881 ccagcagcct ttgatcagaa aaaattagac tggatgagca atgattatat caagaatgca
    35941 gaccttgaaa ccatctttga aatggcaaaa ccattcttag aggaagcagg ccgtttgact
    36001 gacaaggctg aaaaattagt tgagctctat aaaccacaaa tgaaatcagt agatgagatt
    36061 atcccattga cagatctttt cttctcagat ttcccagaat tgacagaagc agagcgcgaa
    36121 gtcatgacgg gtgaaacagt tccaacagtt cttgaagcat tcaaagcaaa acttgaagcg
    36181 atgacagatg atgaatttgt gacagaaaat atcttcccac aaattaaagc agtccaaaaa
    36241 gaaacaggta ttaaagggaa aaatcttttc atgcctattc gtatcgcagt ttcaggcgaa
    36301 atgcatgggc cagaattacc agatacaatt ttcttgcttg gacgtgaaaa atcaattcag
    36361 catatcgaaa acattctaaa agaaatctct aaataaagag gatacaataa tggacatctg
    36421 ggaaaagatg tacgaagaag cacagaaact atacaatcca catgaagtat cagactttgt
    36481 ttatgccaat catgttgtag ctgcagtaga agcaggagat ggacaaatat ttacaggatt
    36541 ctgtatggag ggaacctgtg gtgttttcca tctctgcgca gaacgggcga ccctcttcaa
    36601 tatgtaccaa ttttcaggac aaactaaggt taagaaagta ttagcctttc gagacaaacc
    36661 accttatggt ggaagttcag ccatgccttg cggtgcttgt agagaattcc ttttagagct
    36721 taatgctgaa aataaagatg cagaattcat gatggactat aatataagaa aaacagttaa
    36781 agtcgcagaa ctaatccctt attggtgggg agaagaacgt gcttctaagt ttaatgagtg
    36841 atagaagagt cagtataatt ctggctcttt ttactataag ttgaaaaaat aaaaaaaatt
    36901 agaaaaagta gttgacaaa
//
LOCUS       DAYYQN010000011        35953 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYYQN010000011 DAYYQN010000000
VERSION     DAYYQN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.011"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..119
                     /locus_tag="ACHBRQ_000524"
     CDS             <1..119
                     /locus_tag="ACHBRQ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFM9592242.1"
                     /translation="KNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            514..1377
                     /locus_tag="ACHBRQ_000525"
     CDS             514..1377
                     /locus_tag="ACHBRQ_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HFM9592243.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTALFE
                     LRLQQYKKLID"
     gene            1663..1896
                     /locus_tag="ACHBRQ_000526"
     CDS             1663..1896
                     /locus_tag="ACHBRQ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592244.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            1918..2595
                     /locus_tag="ACHBRQ_000527"
     CDS             1918..2595
                     /locus_tag="ACHBRQ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9592245.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            2607..3272
                     /locus_tag="ACHBRQ_000528"
     CDS             2607..3272
                     /locus_tag="ACHBRQ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9592246.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3344..4570
                     /locus_tag="ACHBRQ_000529"
     CDS             3344..4570
                     /locus_tag="ACHBRQ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9592247.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4805..5089)
                     /locus_tag="ACHBRQ_000530"
     CDS             complement(4805..5089)
                     /locus_tag="ACHBRQ_000530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592248.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHRFEVVDRTDEVS
                     SKHRFEVVDRTDEVSSKHRFEVVDRTDEVSNHTYGKVKLTWFEESFEEYK"
     gene            5160..5816
                     /locus_tag="ACHBRQ_000531"
     CDS             5160..5816
                     /locus_tag="ACHBRQ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9592249.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            5806..6129
                     /locus_tag="ACHBRQ_000532"
     CDS             5806..6129
                     /locus_tag="ACHBRQ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9592250.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            6233..7063
                     /locus_tag="ACHBRQ_000533"
     CDS             6233..7063
                     /locus_tag="ACHBRQ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9592251.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7217..8071
                     /locus_tag="ACHBRQ_000534"
     CDS             7217..8071
                     /locus_tag="ACHBRQ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9592252.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8171..9544
                     /locus_tag="ACHBRQ_000535"
     CDS             8171..9544
                     /locus_tag="ACHBRQ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFM9592253.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            9537..10598
                     /locus_tag="ACHBRQ_000536"
     CDS             9537..10598
                     /locus_tag="ACHBRQ_000536"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9592254.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMAKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            10600..11292
                     /locus_tag="ACHBRQ_000537"
     CDS             10600..11292
                     /locus_tag="ACHBRQ_000537"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9592255.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11322..11891)
                     /locus_tag="ACHBRQ_000538"
     CDS             complement(11322..11891)
                     /locus_tag="ACHBRQ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9592256.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPTPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12026..12640
                     /locus_tag="ACHBRQ_000539"
     CDS             12026..12640
                     /locus_tag="ACHBRQ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9592257.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12618..14288
                     /locus_tag="ACHBRQ_000540"
     CDS             12618..14288
                     /locus_tag="ACHBRQ_000540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9592258.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HMGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14300..15586
                     /locus_tag="ACHBRQ_000541"
     CDS             14300..15586
                     /locus_tag="ACHBRQ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9592259.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15638..16171
                     /locus_tag="ACHBRQ_000542"
     CDS             15638..16171
                     /locus_tag="ACHBRQ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592260.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            16249..16719
                     /gene="nrdI"
                     /locus_tag="ACHBRQ_000543"
     CDS             16249..16719
                     /gene="nrdI"
                     /locus_tag="ACHBRQ_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9592261.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17012..18274)
                     /locus_tag="ACHBRQ_000544"
     CDS             complement(17012..18274)
                     /locus_tag="ACHBRQ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFM9592262.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18584..19042)
                     /locus_tag="ACHBRQ_000545"
     CDS             complement(18584..19042)
                     /locus_tag="ACHBRQ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HFM9592263.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(19039..19479)
                     /locus_tag="ACHBRQ_000546"
     CDS             complement(19039..19479)
                     /locus_tag="ACHBRQ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFM9592264.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19685..21634
                     /gene="mutL"
                     /locus_tag="ACHBRQ_000547"
     CDS             19685..21634
                     /gene="mutL"
                     /locus_tag="ACHBRQ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9592265.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(21959..22426)
                     /gene="ribE"
                     /locus_tag="ACHBRQ_000548"
     CDS             complement(21959..22426)
                     /gene="ribE"
                     /locus_tag="ACHBRQ_000548"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFM9592266.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(22427..23632)
                     /locus_tag="ACHBRQ_000549"
     CDS             complement(22427..23632)
                     /locus_tag="ACHBRQ_000549"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFM9592267.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(23652..24287)
                     /gene="ribE"
                     /locus_tag="ACHBRQ_000550"
     CDS             complement(23652..24287)
                     /gene="ribE"
                     /locus_tag="ACHBRQ_000550"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFM9592268.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(24272..25372)
                     /gene="ribD"
                     /locus_tag="ACHBRQ_000551"
     CDS             complement(24272..25372)
                     /gene="ribD"
                     /locus_tag="ACHBRQ_000551"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFM9592269.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNGAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(25507..25621)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25778..26371
                     /gene="ruvA"
                     /locus_tag="ACHBRQ_000552"
     CDS             25778..26371
                     /gene="ruvA"
                     /locus_tag="ACHBRQ_000552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9592270.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASVENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            26381..26944
                     /locus_tag="ACHBRQ_000553"
     CDS             26381..26944
                     /locus_tag="ACHBRQ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9592271.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPVKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            26941..27618
                     /locus_tag="ACHBRQ_000554"
                     /pseudo
     CDS             26941..27618
                     /locus_tag="ACHBRQ_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
     gene            complement(27759..28790)
                     /locus_tag="ACHBRQ_000555"
     CDS             complement(27759..28790)
                     /locus_tag="ACHBRQ_000555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9592272.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDEIFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(29045..29728)
                     /locus_tag="ACHBRQ_000556"
     CDS             complement(29045..29728)
                     /locus_tag="ACHBRQ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9592273.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPH
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(29740..30684)
                     /locus_tag="ACHBRQ_000557"
     CDS             complement(29740..30684)
                     /locus_tag="ACHBRQ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9592274.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            30816..33647
                     /gene="uvrA"
                     /locus_tag="ACHBRQ_000558"
     CDS             30816..33647
                     /gene="uvrA"
                     /locus_tag="ACHBRQ_000558"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9592275.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKNVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMTAFGVDMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            33640..34701
                     /locus_tag="ACHBRQ_000559"
     CDS             33640..34701
                     /locus_tag="ACHBRQ_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9592276.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            35017..>35953
                     /locus_tag="ACHBRQ_000560"
     CDS             35017..>35953
                     /locus_tag="ACHBRQ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HFM9592277.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSE"
BASE COUNT        10356 a         6869 c         7819 g        10909 t
ORIGIN      
        1 aaaagaattt tgaactattt acaaaacaaa gtgatctaat tgttgcaaat agatgggata
       61 ggggccttga agcttataaa gataaagttt acacaagggg gatctggata agagattaac
      121 tattgatatt aaaaaggcaa taatttataa aatttgtaca aaaaaatgtt aaattttcac
      181 attttcatag tatactgttt agtagaaagt aggttctttg taaccgctcc attataaaat
      241 cctttcactc ttccggtata ctattaggaa aaacataact ggaggatatt ttgagctcac
      301 acctcatcgt tccagtggct gttccccaag ctcgtgtact gcccaaaaaa gttagacatt
      361 ctatttagaa cgaggattga gttctgtatt gtacaaggct cggtcctttt agagtcagct
      421 taaggctggc tttttcaatc acaaaagtgt cagaatgttt tgacaaatga acacaaataa
      481 tgatataata tgcaaaagct aggaggtggt aggatgattg aaaaaatgga actgggggaa
      541 ttttacaagg aattacgttt ggcgagaaag cttaagcaga cagatgtggc ttgtgagggt
      601 ctaacagctt ctcagttgtc caagtttgaa ctagggcagt ctatgctgtc tgcggataag
      661 ttgattctag ctatccaagg tataaatgtg acctttgatg agtttgggca caagctcaac
      721 aactatcaag aatctccaca tatgcgaatc ggtcgaaagg ttgtgaatcg ctttgcccat
      781 caggatatag ctgctttaga gcagctattg gaggaagtcg atcaagaaca gatggcgcag
      841 acctatcgtc gtttgaatgc catcgtgatt aaagatgcca tccattcact aaataaaagc
      901 tacccactag cagaggagga tagcgagttt ttgaccacct atctctatgc tatcgagtct
      961 tggacctggt ttgaactcta tctcttttgc aataccatgc ccttcttgag caatcaagat
     1021 ctgatttttt tatcaacctc cttactcgaa aaatccaaag aatttaaaga gttagtacac
     1081 aatcgattgt atatgaagca aggactctta aatatcttat cagaactcat ggagcgaaaa
     1141 cttttctcct acatcccaat cttcgaagcc gagttggaga ggatgttacg accgtatgat
     1201 gtttttgaaa aagtatcgtg gcaattttta aagaagatga gtgtctttct tcaaacaaag
     1261 ggaagcaatc aaaaagagat tgaacgcttt atccaatctc tgcaagtatt agaaaatcca
     1321 caattgacag ccctttttga attgcgtttg cagcaataca aaaagcttat cgattagtta
     1381 gaaatggcaa aataataata atctcagcaa cgattttcaa taatgttaaa atttgtttct
     1441 tcatatttct ccactcctca ttagtagtaa ctttattata ccagagaagt aatcaaattc
     1501 accaaaatcg caaatctgaa atttctataa gaaaaaatat caaatatgcg attttctaaa
     1561 ataagccaat tttcgtgtta tactgtactt gtaaagcact tgaagcaaat cctaggtcgc
     1621 agaagtggtt tacaaatgaa gataattgaa ggagtgtaaa agatgttaaa tttacaattt
     1681 gcagaaacaa tggaattgac agaagctgag ttgcaagatg ttagaggagg caacctcgta
     1741 aattctatgg gaggtggtgg aagaagtggt atatctggtt ggggcgtccc aggaatttac
     1801 cctggttggg gcaatcaagg gatgagtcct aatcgtggag cttttgattg gactatagat
     1861 ttagcagatg gattgtttgg ccgccgtaga agataggctc ttataggaga taataggatg
     1921 aagaaaatga aagaagtgaa atttcaccta gcaactggct tgttgatttt gacttattac
     1981 ctcattttta atgtcacatc agacttagat tttatggttg ctttgtctga taatatgtac
     2041 tatgtgttcc aagttttatt ggtacttatt ctgggaacta tagctacaat tgcttttgtc
     2101 aaaagtgaac attggaaaga gtgtggtcgt ttccagtttc gttggtctta cctgggtgtt
     2161 ttccttcttt cttttttctt attgttcgtg tgggcaaatc taacaaccta tatctttcct
     2221 cgtacccaga atggaagtac agtcgtagaa gtagccacca atttaacagg aattagctat
     2281 tttgttacgc gaatacttta tactagtatt atcgcacctg tatcagaaga ggttgtttgt
     2341 cgtggtcttt tgatgacaag tttatctaaa gttaagagat actatcttga tgttcttgtt
     2401 tccgctgcca tatttggcgc tatgcatgtt ttgcagtatg gttggataac gacagatttc
     2461 ataaagtatt ttggaatggg cttgatattt tgtatgatgt ttcgctacac ccgatccatc
     2521 tattgggcta tagctctgca tgcttcatgg aatagtttct tactcattgt tactttattg
     2581 gtatttggat attagaaagg tgatatatga aaaagataat ctcacaccgc tactttatta
     2641 tagtttttct actagttatt gctgaccaga agttcagtgt tttagtttta cgcagcgacc
     2701 ttgttgctgg gctatctgac tttgcctatt atctgtcgga tatgatgttg aattttcttg
     2761 tggttttatt tgctcttatt gctatgattt ggtcggggaa atggcagaaa atcaatagta
     2821 gaaagtttaa aggatcctat cttttctatt ccttcctagc tctgcttgct tttgttgctt
     2881 ggaattttgt cacctttttt cttttcccac ctactcgaaa tgaaatttct tatcaacatg
     2941 ctgctcctac ttttacagga gctacggcat ttttgatgta ttttttttat cctgtgattg
     3001 caggtcccat ttttgaagac atgatttatc gtggattagt gatgactgct ctggaaaaag
     3061 gaaagaaatg ggggctagat gtacttggtt ccgctgtttt atttggggtc tcgcacatta
     3121 gtaatcacgg ttgggtcttg acagactttg tcttctatat gggtggaggt cttatatttg
     3181 cagtcttatt tagaatgaca aagtcaattt attggcctat tggactgcat atagtctaca
     3241 atggcattgg tcagcttttg atgttactgt aattttggtt gttagtagta tcgcggtcta
     3301 tccttgggga aagattccca tttgaactta agaaggagtt gtgatgaaag tatttcttca
     3361 aaatagagat tttaggcaat taactatcaa ccagtggatt tcaacgcttg gggatacgat
     3421 tttttatctg gcctttctga attatgtggc agatgcgtct tttgcacctt tggcgatttt
     3481 actcattacg atttcagaaa cccttcccca aattttacaa atctttctgg gagttttggc
     3541 agattttcaa catcatcgtg tcttgaaata cacagtcatt agttttgcaa aatttttgct
     3601 ttactctatc gtttctttat cactttcagg gcagtctttc tccttgttgc tagtagcatt
     3661 tatttgtctg cttaacctct tgtctgacac attgagttac ttttcaggag ccatgctcac
     3721 tccgattttc attagaatta ttgggcaaga ccatctggca gaagctattg gatttaaaca
     3781 gtcaactgtt agtttagtga aaacaatcag taatattcta ggaggagtct tgctagggat
     3841 tctctccatc cagttcattt ccttgctgaa cgctcttacc tttttaatcg catttttagg
     3901 tatcctcttc ataaaaactg acctcttgaa agtagaaaaa acgattaact atcaagaagg
     3961 actctctgta aaatcctttt gccagcattt gctccaatca tcgaaattga tatggaatat
     4021 gaataaggtg ctcttggttt tgtttattat ctctactagt caagcagtga taaatgttac
     4081 agttcctgtc tctactctat ttttgaggaa tcaacccttt ttgaatttac aaacaggtca
     4141 atctcttgcc ttgctatcca cttttgaact gtcagccctg attgtcggaa gccttgtaag
     4201 tggctatctg caacatacca tttccataaa aacagctctc tatgcctcac ttgtcatcca
     4261 gttgcttctt ctagtaggat ttgccacagt tcgttttgac tggattctca tctttagtac
     4321 cttggatgcc tttttcgcag gtgttctctc tcctagattg caagagctcg tctttaaaca
     4381 aatacctgag gagtcaatgg gagcagttca atcctctatc ggtgccatta cggttgtttt
     4441 acctagctta tttacaatag ctttggtaac cattgctact agctttggaa ctctggcagt
     4501 tagctttgtt ttattgctat ttcttctagt tgcctttgtt atgcttctga atattcgtga
     4561 aagtatttag tggagaattt ctataatata tgtttgagac tggattgcca gtctttttat
     4621 gatggtcaaa atcaacagac ttttttctct tttctgaatg tgttataata gactgaatct
     4681 aaaatagtac gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga
     4741 tttgttctca tcttatttca atctgctata taatagtcca tgctgtggct ggattctttt
     4801 cagcctactt atactcttcg aaactctctt caaaccacgt cagcttcacc ttgccgtagg
     4861 tatggttact gacttcgtca gttctatcca caacctcaaa acggtgtttt gagctgactt
     4921 cgtcagttct atccacaacc tcaaaacggt gttttgagct gacttcgtca gttctatcca
     4981 caacctcaaa acggtgtttt gagctgactt catcagttct atctgcaacc tcaaaacagt
     5041 gttttgagca acctgcggct agtttcctag tttgctcttt gattttcatt gagtattatt
     5101 aagacaatat gaggagaaag atgaaagaaa aaggatttta ggagggggcg caggcagcca
     5161 tgccaacggc tcttggctat gtcagtatcg gcctggcctg tgggactatc ggtgcgccct
     5221 atgtgacacc tgttgagatg ggcttgatga gtctctttgt ttatgctggg agtgcccagt
     5281 ttgccatgtt ggcactgatt gtggttcaag ctcctgtggc agctattgct atgacggttt
     5341 ttctaatcaa cttgcgtctc tttttgttga gtttacacgc atcgacttat ttccgtcata
     5401 ccagtctttg gtacaatatc ggtatgtcta gtatcttgac agatgagacc tatggcgttt
     5461 tgatgggtga attggcccat acagacaagg taaatcctat gtggatgcac ggaaacaatc
     5521 ttaacagcta tgtggcttgg tttgtgggga cagtagtcgg aacggctctg ggtggcctgc
     5581 taccaaatcc agaaatcttt ggcttggatt ttgccctggt tgggatgttt attggtattt
     5641 ttgcttcgca atttcagatt atgcaaagac ggattcctgt ccgcaatctg ctcattatcc
     5701 tagcagttgt tgcggtgtcc ttctttttgc tcttgacagt ggtgtctcag tcactagctg
     5761 ttctgtttgc gacgctactt ggttgtagca tgggggtggt tttagatggt cagtaagtat
     5821 cttttattag cagttatttt ctctggcttg gtgacttgga ttctccgtat gattcccttc
     5881 atcttagtca agtataaggg cttgcctgca atcgttgagc gttttttgaa gttcttgccc
     5941 gtttccatta tctttgcctt gattctttca agcgtagtga caggtaaggt tgggagcctt
     6001 cctcaaatta aatggctaga cttcttagcc gtctttccaa cagcttgggt agcctttcgc
     6061 taccgcaatc tagtcggaac agttctcttt ggagtggtct tgattgccat cttgcgtttg
     6121 gtcttttaaa ttacccataa aaaaaactta tcacagagat agatatcata taatggcgta
     6181 aatgctcttt ttctgttaag attataaggt attctatttt ggaggaaatg acatgaaaaa
     6241 aatcgttaaa tactcatctc ttgctgccct aggacttgtt gctgcaggtg tgcttgcggc
     6301 ttgctcaggg ggtgctaaga aagaaggaga agcagctagc aagaaagaaa tcatcgttgc
     6361 aaccaatgga tcaccaaggc catttatcta tgaagaaaat ggcgaattga ctggttacga
     6421 gattgaagtc gttcgcgcta tctttaaaga ttctgacaaa tatgatgtca agtttgaaaa
     6481 gacagaatgg tcaggtgtct ttgctggtct tgatgctgac cgttacaata tggctgtcaa
     6541 caatcttagc tacactaaag aacgtgcgga gaaatacctc tatgccgcac caattgccca
     6601 aaatcctaat gtccttgtcg tgaagaaaga tgactctagt atcaagtctc tcgatgatat
     6661 cggtggaaaa tcgacggaag tcgttcaagc cactacatca gctaagcagt tagaagcata
     6721 caacgctgaa cacacggaca acccaactat ccttaactat actaaggcag atttccaaca
     6781 aatcatggta cgtttgagcg atggacaatt tgactataag atttttgata aaatcggtgt
     6841 tgaaacagtt atcaagaacc aaggtttgga caacttgaaa gttatcgaac ttccaagcga
     6901 ccaacaaccg tacgtttacc cacttcttgc tcaaggtcaa gatgagttga aatcatttgt
     6961 agacaaacgc attaaagaac tctacaaaga cggaacgctt gaaaaattgt ctaaacaatt
     7021 cttcggagat acttatctac cagcagaggc tgatattaaa taatttcttg aaatcatcca
     7081 ttctgagtaa gatgggtgat ttctcagttt ttagggatat agttttaaac tatatatctg
     7141 attatctaat aacaatttgt gaaatcaaac aaattgtgag atactagtac ggtattattt
     7201 ttaaggagaa agaatcatga aaatcaaaaa atggcttggc ctagtagccc ttgctacagt
     7261 cgcaggtttg gctcttgcag cttgcggaaa ctcagaaaag aaagcagaca atgcaacaac
     7321 tatcaaaatc gcaactgtta accgtagcgg ttctgaagaa aaacgttggg acaaaatcca
     7381 agaattggtt aaaaaagacg gaattacctt ggaatttaca gagttcacag actactcaca
     7441 accaaacaaa gcaactgctg atggcgaagt agatttgaac gctttccaac actataactt
     7501 cttgaacaac tggaacaaag aaaacggaaa agaccttgta gcgattgcag atacttacat
     7561 ctctccaatc cgcctttact caggtttgaa tggaagtgcc aacaagtaca ctaaagtaga
     7621 agacatccca gcaaacggag aaatcgctgt accgaatgac gctacaaacg aaagccgtgc
     7681 gctttacttg cttcaatcag ctggcttgat taaattggat gtttctggaa ctgctcttgc
     7741 aacagttgcc aacatcaaag aaaatccaaa gaacttgaaa atcactgaat tggacgctag
     7801 ccaaacagct cgttcattgt catcagttga cgctgccgtt gtaaacaata ccttcgttac
     7861 agaagcaaaa ttggactaca agaaagcact tttcaaagaa caagctgatg aaaactcaaa
     7921 acaatggtac aacatcattg ttgcgaaaaa agattgggaa acatcaccta aggctgatgc
     7981 tatcaagaaa gtaatcgcag cttaccacac agatgacgtg aaaaaagtta tcgaagaatc
     8041 atcagacggt ttggatcaac cagtttggta ataagaaaca gggaggtggg agagagaatt
     8101 ccacctcttg cttttgtata gagcacggag tgtaaagaag actattcgtt catagaaagg
     8161 tagagagaat atggtttttc ctagtgaaca agaacagatt gaaaaatttg aaaaggatca
     8221 tgtagcccag cattattttg aggttttgcg taccttgatt tctaagaaat cagtctttgc
     8281 ccagcaggtt ggactcaagg aagtcgcaaa ttatctgggt gagattttca agcgtgttgg
     8341 agctgaagtg gagattgatg agagctatac agcgcccttt gtcatggcac atttcaagag
     8401 ttcgcgtcca gatgccaaga ccttgatttt ctataaccac tatgacactg tgccagcgga
     8461 tggggatcag gtctggacag aggatccttt tacgctttcg gtccgcaatg gcttcatgta
     8521 tgggcgtgga gttgatgacg acaagggtca tatcacagct cgcttgagtg ctttgagaaa
     8581 atatatgcag caccatgatg atttacctgt caatatcagc tttatcatgg agggagcgga
     8641 ggaatcggct tcaacagacc tagataagta tttggaaaag catgcagaca aactccgtgg
     8701 ggcggatttg ttggtctggg aacaagggac caaaaatgcc ttggaacagc tggaaatttc
     8761 tggtggcaat aaggggattg tgacctttga tgccaaggta aaaagcgctg atgtggatat
     8821 ccactcgagt tatggtggtg ttgtggaatc agctccttgg tatctcctcc aagccttaca
     8881 gtctcttcgt gctgcggatg gccgtatctt ggttgaaggc ttgtacgaag aagtacaaga
     8941 gcccaatgaa cgagaaatgg ccttgctaga aacttatggt caacgaaacc cagaggaagt
     9001 tagtcggatt tatggattgg agttgcctct cttacaggag gagcggatgg cctttctaaa
     9061 acgtttcttt ttcgagccag cgcttaatat cgaaggaatc cagtctggtt atcaaggtca
     9121 gggtgttaag actattttac ctgcagaagc cagtgccaag ctagaggttc gtctggttcc
     9181 gggcctagaa ccgcatgatg ttctggaaaa aattcggaaa cagctagaca aaaatggctt
     9241 tgataaggta gaattatact ataccttggg agagatgagc tatcgaagcg atatgagcgc
     9301 accagccatt ctcaatgtga tcgagttggc caagaaattc tatccacagg gcgtttcagt
     9361 cttgccgacg acagcgggga caggacctat gcatacggtc tttgatgccc tagaggtacc
     9421 aatggttgca ttcggtctag gaaatgccaa tagccgagac cacggtggag atgaaaatgt
     9481 gcgaatcgct gattattaca cccatatcga attagtagag gagctgatta gaagctatga
     9541 gtagagatat tatcaagtta gatcagatcg atgtgacttt tcaccaaaag aagagaacca
     9601 ttacagcggt taaggatgtg accattcaca tccaagaagg ggatatctac ggaatcgttg
     9661 gatattctgg agcagggaaa tcaacccttg tacgggtgat taacctcttg caaaaaccat
     9721 ctgcagggaa aattaccatt gacgacgatg tgatttttga cggcaaggtg accttgacgg
     9781 cagagcagtt gcgtcgtaaa cgtcaagata tcgggatgat tttccagcat tttaacctga
     9841 tgagccaaaa gacagcagag gagaatgtag cctttgccct taaacactct ggactcagca
     9901 aggaagaaaa gaaggctaaa gtagctaagt tgttggactt ggttggtttg gcagatcgtg
     9961 ctgaaaacta cccttcacaa ctatctggag ggcaaaaaca gcgtgtggca attgcgcgtg
    10021 ccttggccaa tgatccaaaa atcttgattt cagacgagtc aacttctgcc cttgacccta
    10081 agacaaccaa gcagattttg gccttgttac aagatttgaa ccaaaaatta ggattgacag
    10141 ttgtcttgat tacgcatgaa atgcagattg tcaaagacat tgccaaccgt gtggcggtta
    10201 tgcaggatgg gcatttgatt gaagagggaa gtgtccttga aatcttctca aaccctaaac
    10261 aacctttgac tcaagacttt atctcaacag ccacaggtat tgacgaagcc atggccaaaa
    10321 tcgagaagca agaaatcgtg gaacacttgt ctgaaaacag tctcttggtg caactcaagt
    10381 acgctggagc ttcaacagac gagccacttt tgaatgaatt gtacaagcat taccaagtaa
    10441 tggctaatat tctctatggg aatatcgaaa ttctcgatgg tactcctgtt ggagaattgg
    10501 tggtggtctt gtcaggtgaa aaagcagcgt tggcaggtgc ccaagaagct attcgccaag
    10561 caggtgtaca actaaaagta ttgaagggag tacagtaaga tggaatcatt gattcaaacc
    10621 tatttaccaa atgtctataa aatgggttgg gctggtcagg caggctgggg aacggctatc
    10681 tacttaactc tttatatgac agttctttcc ttcattatcg gaggcttctt ggggctagtg
    10741 gcaggtctct ttctcgtctt gacagcgcca ggtggtgtct tggagaataa agtcgtattc
    10801 tggattttag acaaaattac ctcaattttt cgtgcggttc cctttatcat cctcttggca
    10861 atcttgtcac cactttctca cttgattgtt aaaacaagta tcgggccaaa tgcagccctt
    10921 gtcccacttt cttttgcagt ctttgccttc tttgcccgtc aggtgcaggt tgtcttggct
    10981 gaattggatg gcggtgtcat tgaggcggct caagcgagcg gagcgacttt ctgggacatc
    11041 gtgggtgttt acctatcaga aggtcttcca gatttgatcc gtgtgacgac tgtgaccttg
    11101 atttcccttg ttggggaaac agctatggcc ggtgcggttg gagctggtgg tatcggtaac
    11161 gtagccatcg cttatggatt taaccgctac aatcacgatg tgaccatctt ggcaaccatc
    11221 attatcattt tgattatctt tgcaatccaa ttcttgggag atttcttgac taagaaattg
    11281 agccataaat aaaaaagagc cgcgtggctc tttttaactg atcagatttt ctgtgcaaat
    11341 tttttactca aggcttgtcc aatcaaggca cccactaggg ctccgatgac aatacttgcg
    11401 ataaatagaa ggacagttcc aggggttgga gcgaccatga tacggtcgat atattcttgg
    11461 gattttcctc ttgccagaag agtagccata taggctttgg gcgcaatcca cataagcaag
    11521 attggtcctg ttgtactaaa ggcgaaaata atgaaagaaa ggaagttctt tgttttgtcc
    11581 ttgtattttc ctaaatgagc tactccatct gctaggaggc cacagataat tccaggaagg
    11641 aaggaaccag caccgtgttt agttcccaag aaaaagaggg caatgacaag gccgatagtg
    11701 gtaatggctc caaagcgcgg aacttttgcg actaggatca tatagacgct accgccgaca
    11761 agggcagtaa aggcaggcgc ataaaacatg tttccagttt ggtcaaagag attgcccaaa
    11821 aggacaccaa tccccatgca gaggaagtaa agaactgcaa aaagcagtgt agttaagata
    11881 gatttcttca tgaattgtct cctgataatt ttttcacaat tctcatttta ccatggtttg
    11941 gtgggattgt aaatggatac tagaagtttt tgaaaacgct tgaaatatga tataatggtt
    12001 tcatagttat ttttagggga atatcatggg tagattttta gactttgtct ttaatcgttt
    12061 ctttttaggg atgattgcga cagccttctt ttggctatta actttagcag gagggattat
    12121 ccttggtcta gcgccggcta gtgccacctt gatgagctta tatgcagaac atggttatag
    12181 ctttcgggaa tacagtttga aggaggcttg gtctctttac aagcaaaatt ttgtctcaag
    12241 caacctgatt ttctatagct ttttaggtgt gggtctagtt ttgacctatg gtttgtatct
    12301 cttggtgcaa ttgcctcatc agaccattgt tcatttgatt gcgacccttt tgaatgtcct
    12361 agtagttgcc ctgatctttt tggcttatac agtatcttta aaattacaag tttattttgc
    12421 cttgtcctat cgaaatagtc tcaaattatc cttgattggc atctttatga gtctagcagc
    12481 tgtggccaag gttctccttg ggactgtgct acttgtagca attggttact atatgcctgc
    12541 cctgctattt tttgtaggaa ttgggatgtg gcatttcttt atcagtgata tgttggaacc
    12601 tgtctatgaa atcatccatg aaaaattggc gacaaaatag aatgaagcag ttttggctac
    12661 atacgcttct aagaacctat agttcagtga tgatcattat cattgcgagt tttgcaatct
    12721 tactctctta cgctgactgg gattcacgtg aaaaggaagc ccagagagta gcccagcgtg
    12781 taactgctag aacagtgagt gaaattgaat attaccatag agagtcaacc cagatagctc
    12841 aggctttagt tgaaaatcaa gctcgtattg agggaatcta taaatacttt agccttagca
    12901 tgccagacta tttttactgg caattagagc ggaaagcttc gccttatata tcagtctctc
    12961 tgtatgaaaa tgttgatgac ctctatgttc gaaatgattt tgtaactgga gtggccattg
    13021 cttttcaaga ttacaaggaa gtctatgttt ctactaaaga caaacgtagt ggagaaaaaa
    13081 tcagggctga ggatttcaaa ccagcaggaa atagttttgc cattccagtg tcagatccag
    13141 tgtcagatca agacttagga gtgatttaca tctccttgga tcctgctgtt ttataccatg
    13201 ccattgataa tactagaggt catactccga tggcagtaac agtgacctca ccttttgata
    13261 cggagatttt tcatatgggt gagacagttg ataaggagag tgaaaattgg ctagttggct
    13321 taacttctca tggttatcag gttcaggtgg cagttcccaa aaactttgtt ttacaaggaa
    13381 cggtgaccag ctctgctttg attgtgggct tgagccttct ctttattgtc attctttatc
    13441 tgactttgag gcagaccttt gctaattatc aaaagcaggt agtggatttg gtggattcca
    13501 tccaagctat tgcccaagga caagaaggtc ttcgcattga tacgcttgaa aaggatcagg
    13561 aattgctcct aatcgcggag acgaccaatg atatgttgga tcgattggaa aagaatatcc
    13621 atgatattta ccagttagaa ctcagtcaaa aagatgccaa tatgcgggcc ttgcaggcgc
    13681 aaatcaaccc tcacttcatg tataatacgc tggagttctt gcgtatgtac gcagtcatgc
    13741 agagtcaaga tgagttggca gatatcattt atgaattcag tagtctcttg cgtaacaata
    13801 tttccgacga aagagagacc ctcctcaaac aggaattaga attttgccgt aaatacagct
    13861 atctctgcat ggttcgctat cccaagtcca ttgcctatgg tttcaagata gatccagagt
    13921 tagagaatat gaagattccc aagtttacct tgcaaccgct ggtagaaaac tatttcgcgc
    13981 atggtgttga ccacaggcgg acagataatg tgattagcat caaggctctt aaacaggatg
    14041 ggtttgtgga aattttggtg gtcgataatg gtagaggaat gtcggctgaa aagttggcaa
    14101 atatccgaga aaaattaagt cagaaatatt ttgaacacca agccagctac agtgatcaaa
    14161 ggcagtctat cgggattgtc aatgtacacg agcgttttgt gctctatttt ggagaccgct
    14221 atgccattac tatagagtct gcagagcaag ccggtgttca gtatcgtatt acaattcaag
    14281 atgagtagaa agggagaaaa tgtataaagt attattagta gatgatgagt acatggtgac
    14341 agaaggtctg aagcgtttga ttccctttga taagtgggat atggaggtcg tcgcaacagc
    14401 cagtcatgcc gatgaagctc tagaatatgt tcaggaaaat cctgtcgatg tcatcatttc
    14461 cgatgtcaat atgccagaca aaacagggct tgatatgatt cgggagatga aagagatctt
    14521 accagatgct gcctatatcc tgctctcagg ttatcaggag tttgattatg taaaaagagc
    14581 aatgaacctt agtgtggtgg actatttggt caagcctgtt gataaggtag agctgggaaa
    14641 tctgctggag aagattgcag gtcagctcgg cgagagaggg aagaaaagtc agactcttag
    14701 tcaagaatta gacgaggctg gatttgttag ttatttaggg gataaggaga attggtggat
    14761 aggtctatcc aaggaaaaac aaggttcctt caccattccc tactatgtct tgggccaaga
    14821 ctggcagatt ttcatttctg accaacccct agatggttta gtcgttacac cttttgaagc
    14881 tccttatcaa gaacactttg aacgctggaa gctgaatgct gagaaaaccc tcttttacgg
    14941 ttctgtaaat ttgcagcagt ctgagagtct ctttgcctat tacgaaccga tttatagggt
    15001 tattattcag ggaaatctca atcaaatcgt agaagagtta aatctcttgg agaaggtagt
    15061 tcttgaaaat acgccgcgtg ttccgattac taaacagctt tttattcagt ttgtcatgga
    15121 tgttttccat ttatttgaac atctcaaagc tgatgacctg acagacatcg tcaaaaccat
    15181 tcatgctatt caatccttcg atgaattggt tccttatatc aaggaaactc tgagcagctt
    15241 tttcggtcaa taccgtatga atgaaaatgt ggtcagtgtg ctggaagtca ttggtcgtga
    15301 ttaccaaaaa gagctttccc tcaaggatat cagtaaggct ctctttatca atcctgtcta
    15361 tctagggcag ttgattaagc gtgaaaccga ttcgactttt gcagagttac taaacaaaca
    15421 acgtattaag gctgcccaac aacttttgct ttcaactagt gacagcatcg aagatatttg
    15481 ttatgctgtt ggttacagta accttggata tttctataaa gttttccgaa aattgtgcgg
    15541 aaaatcgcca aaagcctacc gaaaacaggt agaaactata ctataagatt tgtattcctt
    15601 tacaaaaggt gctataatat tatcaggagg tcctatcatg aaaaagaaac caatttatct
    15661 atgggtcttg ctaatcttgt ctgcccttat ttcagttccg tctctgtttg gaattgtgag
    15721 tcccttgcct agtaaagaag cccttcgtgc tgctcagaaa caagttgcag gggtcaatgc
    15781 tcagcaatta gaagaccagc ttaattatac ctatagagta gcagaagcat ctcattctat
    15841 ttttaatgtt gctttgattg tgctatctac tattttagtg gtcgtagcga ttgtcttcct
    15901 tgttcgtaaa aatttgcaat atgcaaacta tacttacgtt ggctatgttt tgttagctat
    15961 tattggttcg atttatggct atgttggttt acaagacgct gtgcagttgg ttcaagatga
    16021 gagcatgcgt ttgacagtga gtattggctc aaaagctgtt agcattttct atatcgtcat
    16081 caatgttctc ttcctagccc ttgtcttcta taagatgtgg cgacaacaga aagccttggc
    16141 agaagaagag gaaacagaag agcttaccta agtattgaca aaaaagaact atcaagatac
    16201 aatcttggta gttgtttttt gattatagat aaaatcatgg aggtacatat gaagacaatt
    16261 tctttagttt atattagttt gagtggcaac actgaaagtt ttgtgacgcg cttgaaagac
    16321 tatctcttgt cccagtacaa agggattgag gttcaaaaga ttcatatcaa ggatttggtc
    16381 aaggaaggca aaaatttcta tgaaatggac catccctatg tcgctttttt gccgacctat
    16441 ctcgaaggtg ggaatggcgt ggataacgga gatgttgaga ttttgacgac accggtggga
    16501 gattttattg cctatggtaa caatgctagt aagtgttttg gtgtggttgg gtcaggaaat
    16561 cgtaacttta ataaccaata ctgcctaaca gccaagcaat acagtcaacg ctttggcttc
    16621 cctgtattgg ctgactttga aatgcgaggc atgctggaag atatcaaaca tgtcgcagct
    16681 attatcgcag atttgtatga gttggaaaag gagaattaat actcaatgaa aatcaaagag
    16741 caaactagga agctagtcgc aggttgctca aagcacagct ttgaggttgc agatagagct
    16801 gacgtggttt gaagagattc tcgaatagta taaagttctt ataagaggtg gttgaagttg
    16861 ttcaactgtt ttttgagtat aaacagtctt taagaacgta aaaaagcata agttcaggtc
    16921 ttcaaaacct tgttcttatg ctttttatca tagaaatatt tgctgaattt tctcttgaaa
    16981 tgaaacctta tcttcatctc gcgcttgtcc cttaccctgc ttttccgatt gcgagtgcgt
    17041 agataaagaa gatggcgaag ccaaagagga aaatcaatcc cgcaatggca aggtgtttga
    17101 gccaagaagg aagatttttg ttttcacgtg ttaccaaggc gtaattcaaa agagcaaaga
    17161 aaggtgttgt caggaaagag ccaatcatgg caaagcggag catggttgaa acctgaccag
    17221 cgaagaactt gataatgacg ataccgatga tagcagtgat ggtcatccag atgttcaaag
    17281 atttacgatt gtcctctttt tgactgatta gcagtcggag agattcctga ttaacgcgag
    17341 aatagccatc gataacagtt ataactgttc caaagataca gaggaaggca ataaaggtaa
    17401 tcaagtaacg ggaccattcg ccaagaacag aggcatacat gcccacgaat tgagagatgt
    17461 atttggctga agcagcttca accgcctgcc ctgtaggata ctgaatcagt gctcccagtg
    17521 ccacaaagaa gacggctagg atagctgttc caatataacc agtgttaaag tcaaacagag
    17581 cgtcttctgt gttaaagttg acggtctttc tcttttcagc tgaccaaagt gaattgatgg
    17641 ctgaaatttc aataggagcc ggcatccatc ctaggaggga gacgatgaag ggcagagctg
    17701 ccatttgcca aggtgtcttc tcgacaaaat cagaactgta ttctggatgc ttgaccgccg
    17761 caatgataac tgcaagaaca gtcgcaatgg ttaaggcaga cataatccat ttgaccatgc
    17821 cgtctaaaag tttgtagcct ccaaagagta gcatagccca aatgattgca acgagaatga
    17881 gggaccactg agtaatgcta agtccaatca ttgggaaggc actggcgatg atagctgagc
    17941 acagaatggc aacaccagcc gtgttgacca tagccgaaaa gacattgaga ataaagaaaa
    18001 tccagagata gagttttcct ttttcggcat aaccttcaac caaagtcttt ccagtatcag
    18061 ctgtgtattc agcaccaaaa cggaaaaatg gatatttaaa gacattggct aagatgacca
    18121 agagaagtag agaccaaccg taagaaccgc cagcttgagt tgaggataca atgtgggaac
    18181 ctccaacagc ggcagttgcc attaggattc caggtcccat tgcttttagc ttgcttgccc
    18241 aggttgattg atttaaggaa atagcttgcg acatgataaa tactcctttt tgaattttca
    18301 gaataatctg tatatgcaat ctatattcta caaaaaattt tggaaaaatg caagaatatt
    18361 ttgaaaaaag atagaaaatt tacaaagaaa atctgaaata actgttatga tatagcagat
    18421 tgaaactaga atagtacact tttgcttcta aaacattgtt agaaatcgat ttgactgtcc
    18481 tgattgattt atcctgttct tatttcattt cactatagta aggtgcagca ggataaagca
    18541 aaaccgagaa gtttctttct cggttttgct tattatgagg ttcctatttt ctatgctcca
    18601 agagttgatt gatgtgatct acttttttag cctctctttt atagagaata gcccagatga
    18661 tgagatagac aatcgcaaac tcgataatga gctggaggta gaaaatccag tggaagggga
    18721 accaaccagc aagagttgct agtgggacaa agccagccag cataaggaag aaatgagtca
    18781 gagtggcacg aagcatgctc cagtcacggc tgaataagcg gttgccaaag ttgaagagca
    18841 taccgatggc tgcccagata agtgtgcagt agagcaagac cagggcaccg tgaacctgat
    18901 gctgatccat cacttggccg atgagagagt agggatttag tggtgcgtag gtatttggtg
    18961 cataaatgag tgaaaagagg atggagagga tgaggccgat aagaacaccg gcagctgcat
    19021 cgtgaaatac ttgttttttc atagttctaa tttctccttg atggttttta gataacggcg
    19081 tgaagagtag gtgaagcttt cgtttttcaa gaaaatttct accagaccgt ttggcgtgag
    19141 cttgaggtga gagatggaat cgatattgat gatttctgat tgggaaattt ggataaaatt
    19201 gcttggcaag agtttaagaa cctgatagag tcgcaaatca atgctgtagg tctgactcgc
    19261 ggtttctgct agaaccttcc gattctcgat atagaagcgc tgaatcttgc caatctcaac
    19321 tagatagacc tgatcatcga tttttccttt gattttttct ctttggtcca gattttctgc
    19381 gaactcgatg actttctgga ctttttcggt ttcttgaggt gcttggacaa tcagcttttc
    19441 ctcctcgtaa gtctcactaa tctgtagttc tactttcata gttttctctc cttttcagtt
    19501 atacaaggtt gtgatcactt cctgtatatc cttattaaac actattttat agcccatggc
    19561 aaggaacctt gtctatgtgg agggattggg ctcctatgtg gtggagcttt tctgttcttt
    19621 ctgaaatatg gtataatagc actaattaat ttctaggaaa atagatacag aaaggggctg
    19681 aaagatgtct catattattg aattgccaga gatgctggca aaccaaatcg cagctggaga
    19741 ggtcattgaa cgtcctgcca gtgtggtcaa agagttggta gaaaatgcca ttgacgcggg
    19801 ctctagtcag attatcattg agattgagga agctggtctc aagaaggttc aaatcacgga
    19861 taacggtcat ggaattgccc acgatgaggt ggagttggcc ctgcgtcgcc atgcgaccag
    19921 taagataaaa aatcaagcag atctctttcg gattcggacg cttggttttc gtggtgaagc
    19981 cttgccttct attgcgtctg ttagtgtctt gactctgtta acggcggtgg atggtgctag
    20041 tcatggaacc aagttagtcg cgcgtggggg tgaagttgag gaagtcatcc cagcgactag
    20101 tcctgtggga accaaggttt gtgtggagga tctctttttc aacacgcctg cccgtctcaa
    20161 gtatatgaag agccagcaag cggagttgtc tcatatcatt gatattgtca accgtctggg
    20221 cttggcccat cctgagattt cttttagctt gattagtgat ggcaaggaaa tgacgcggac
    20281 agcagggact ggtcaactgc gccaagcaat cgcagggatt tacggtttgg tcagtgccaa
    20341 gaagatgatt gaaattgaga actctgacct agatttcgaa atttcaggtt ttgtgtcttt
    20401 acctgagttg actcgggcta accgcaatta tatcagcctc ttcatcaatg gccgttatat
    20461 caagaacttc ctgctaaatc gtgctatttt ggatggtttc ggaagcaagc ttatggttgg
    20521 acgttttcca ctggctgtca ttcacatcca tatcgaccct tatctagcgg atgtcaatgt
    20581 gcatccaact aagcaagagg tgcggatttc caaggaaaaa gaactgatga ccctggtttc
    20641 agaagctatt gcaaatagtc tcaaggaaca aaccttgatt ccagatgcct tggaaaatct
    20701 tgccaaatcg accgtgcgca atcgtgagaa ggtggagcaa actattctcc cactcaaaga
    20761 aaatacgctc tactatgaga aaactgagcc gtcaagacct agtcaaactg aagtagctga
    20821 ttatcaggta gaattgactg atgaagggca ggatttgacc ctgtttgcca aggaaacctt
    20881 ggaccgattg accaagccag caaaactgca ttttgcagag agaaagcctg ctaactacga
    20941 ccagctagac catccagagt tagatcttgc tagcatcgat aaggcttatg acaaactgga
    21001 gcgagaagaa gcatccagct tcccagagtt ggagtttttc ggacaaatgc acgggactta
    21061 tctctttgcc caagggcgag atggacttta catcatagat cagcacgctg ctcaggaacg
    21121 ggtcaagtac gaggagtacc gtgaaagcat tggcaatgtt gaccagagcc agcagcaact
    21181 cctagtgccc tatatctttg aatttcctgc ggatgatgcc ctgcgtctca aggaaagaat
    21241 gcctctctta gaggaagtgg gcgtctttct agcagagtac ggagaaaatc aatttattct
    21301 acgtgaacat cctatttgga tggcagaaga agagattgaa tcaggcatct atgagatgtg
    21361 cgacatgctc cttttgacca aggaagtttc tatcaagaaa taccgagcag agctggctat
    21421 catgatgtct tgcaagcgat ctatcaaggc caatcatcgt attgatgatc attcagctag
    21481 acaactcctc tatcagcttt ctcaatgtga caatccctat aactgtcctc acggacgtcc
    21541 tgttttggtg cattttacca agtcggatat ggaaaagatg ttccgacgta ttcaggaaaa
    21601 tcacaccagt ctccgtgagt tggggaaata ttaaaagtat aaaaaagtct gggaaaaatt
    21661 ttcaaaatca aaaaaacgca taaaatcagg tgttcaaaaa ccttgatttt atgcgtttta
    21721 tcatggaaat agttacttca ttttttccta attcttttcg aaactctttt taaacgacgt
    21781 cagttttatc agtaatctca aaacagtgtt ttgagctaat tttgccagtt ttgtctgtaa
    21841 catcgaagtt gtgttttacc actctgcgac tggtttccta gtttgctcta tgattttcac
    21901 agagtattaa attgcgattt tgccaagttt ctttattcgt ctaaaagtag agtctgttct
    21961 atgcgtctaa tgtacgaatt aggttgacca tttcaatagc tccttgtgca cactcagaac
    22021 ccttatttcc tgctttagta ccagctcgtt ctatggcttg ttcaattgta tctgtcgtta
    22081 gcacaccaaa cataacagga atttcgctat ttaaactgat ttgggcgatt cccttagata
    22141 cctcgctaca tacataatca taatgacttg tatttcctct aatgacagct cccaagcaga
    22201 taattgcatc atatttttta ctttttgcca tttttgatgc aatcagtgat atttcaaaag
    22261 ctcctggaac ccaggctacc tcgatatctt tctcgtttac attctctctt ttgagattat
    22321 ctagtgctcc agataataat tttgaagtta taaattcatt aaatctcgct acaacaatac
    22381 ctattttaat attgtttgct actaaattac cttcataagt gttcatttat ttttcctcca
    22441 tatttaaaat gtgacccatt cgatttttct ttgtttctaa ataaaaacta tcgtaaggat
    22501 tggcttctat ttcgattgat attctactgg aaatggtaat tccatatttt tctaactgtt
    22561 caaccttgtc aggattattt gtcagtaaat gaagtgactg aagtcccaga tctttaagca
    22621 tttgtgctcc aatatgatat tctcttaaat caccttcaaa gcctaatgca agattggcat
    22681 caagcgtatc catgccttga tcttgtaaat gataggcttt taatttattg ataagtccaa
    22741 ttcctcgtcc ctcctgtcgc aagtaaagta agacacccga accattctca acaatcattt
    22801 tcatagcttt atcgaattgc tgtccacaat cgcaacgtaa ggagcctaaa acatcccctg
    22861 ttaaacattc ggagtggacc cgacataata cattggcttc atcctctatg tttcccataa
    22921 taagagcaag atgatgttcc ccatttagtt tatctatata gctaattgct ttgaaattac
    22981 cgtatctagt aggcatattg acagttgaaa ctcgttctac cagctgatca tatacttttc
    23041 tatattcttg taattctttg atggtaatta gtggaatgtt gtgtttcttc gagaactgaa
    23101 ttaaatcatc tgttctcatc attttgccat catgattcat tatttcacaa cataggccac
    23161 actcttttag tccagctaat tttaataaat caacagttgc ttctgtgtgt ccatttcttt
    23221 ctagaacacc accttttttt gcaattaaag gaaacatgtg tcctggcctg cgaaaatcag
    23281 agggtgttat atcttcagct acacacatac gtgcggtcag tcctctttcc tcggcagaaa
    23341 tacctgtggt cgtttcttta taatcaattg aaactgtaaa agcagtctta tgattatctg
    23401 tattgttttc aaccataggt gaaagcatta attgattagc taaactttcg ctcataggca
    23461 tacaaattaa tcctttggca taagtagcca taaaattaac attttctgtt gtagctgctt
    23521 gtgcagaaca aattaagtct ccttcatttt ctctatcctt gtcgtctata acaagaacaa
    23581 gtcgtccctt ctgcaatgct tctaatgctt cttgtatttt tcgatattcc attgactgat
    23641 tatcctttct gctaaaatcc attttgatat aatagttcct tagatatttc tgattttgga
    23701 gagttatcca tcagtttttg cacatattta cctaagatat cattttcaag atttactgta
    23761 ctcccgactt gtttactctt aagaatggtt tgttccaagg tatgagggat aacagatact
    23821 gaaaagttta ctttggagac tttagcgaca gtcagactaa tgccgtcaat tgtaatagat
    23881 cctttttcaa ctattaaatc taaaatttct ttttgtgtgt tgatttgata ccatacagca
    23941 ttatcatctt tttttattga cgagattttt cctgtaccat caatgtgtcc tgtaacgacg
    24001 tgacccccaa gtcgaccgtt gacagataag gctctttcta gattcacctc acttccatgt
    24061 tttaatagag taagagctgt tcgactccat gtttcattca ttacatcaac tgtaaaggat
    24121 tgatgattga aatgagtaac tgtaagacag ataccattta ctgctatact atcgcctaaa
    24181 tggatatccg ttaatatttt tgaggcttta attgatagtt tacaattacg agagtctttc
    24241 tgtattcttt caacttttcc gatttcttca attattcctg tgaacatgga taaatcactt
    24301 cactttctat gagatagtca tttcctattt gagaaaatgc ataaggtttc aatctaatag
    24361 cgccatttgg caaagaaata ccttcacctc cgacaggaaa cttggcacta cctccaaaaa
    24421 tttttggtgc aatatatatt ttcagctcat caacaatttg ttgttccaaa gcactccaat
    24481 tcattagact gcccccttct agaactaggc tatcaatctg catgtttcct agatgttgca
    24541 ttaaactcga taagtctata tgattgcctt ttttctttat ggaaagtatt tcacagccat
    24601 gattttgata tagcttcatt ttatttttgt cttcagagga agtggcaatg taagttttaa
    24661 tatcatttgc tgtttttacg attttagagg taagaggagt tcgtaaatgt gtatcgcata
    24721 tgatacggat aggatttttc ccttcctcca atctacatgt cagcaaagga tcgtcttgaa
    24781 taacagtatt gactcccacc ataattgcac taacatagtg tcgtaactga tgcacatgct
    24841 ttcttgcttc ttcttcagta atccatttgg attgatttgt tttagtggct atttttccat
    24901 ccattgacat tgcatatttc ataaaaacat agggtacatg ctgggtaata tactttctaa
    24961 aactttttat taagttaaga cactcatttt ctaaaattcc aacagtaact tgaagattat
    25021 tttcctcaag tatctttact ccttttccag atacaatagg attacagtct aggcttccaa
    25081 tgactactct tgtaatacca ctatcgatta tagcatctat acagggaggt gttttcccga
    25141 agtgacaaca gggttcaagt gttacataaa gcgtcgctcc gacaggggat tttctacagt
    25201 ttttaagagc atttctctca gcatgtgggc caccaaaaaa ctcatgataa ccttgtccga
    25261 tgatgtgatt atcttttaca ataactgcgc cgaccatagg attgggattg acgtaaccag
    25321 cccctttttg tgccagtttt attgctaatt tcatatattt tgaatcgctc atctcactac
    25381 ctccaaaaaa aatatacctt gaatagggga ctactcaagg catacaaaag aaaacttata
    25441 cgattaacaa aaatactctg aaatgacaag taatcatttc agagcacgca aaaagcacaa
    25501 atatactttt atcttctttc atccagacta tactgtcggc tttggaattt caccaaatca
    25561 tgcctttcgg ctcgtgggct ataccaccgg tagggaattg caccctgccc tgaagatagt
    25621 tattcaatta cagatgatta tagtacttaa ttttgaatat gtcaacagat aaataccgat
    25681 tgtttttgat atactgtatt tgtgataatc gattctcgct cctcggataa agaaaatatg
    25741 atatactaga taaacgaaat aagagagaag gaatactatg tacgcatatt taaaaggaat
    25801 cattaccaaa attactgcca aatacattgt tcttgaaacc aatggtattg gttatatcct
    25861 gcatgtggcc aatccttatg cctattcagg tcaggttaat caggaggctc agatttatgt
    25921 gcatcaggtt gtgcgtgagg acgcccattt gctttatgga tttcgctcag aggatgagaa
    25981 aaagctcttt cttagtctga tttcggtctc tgggattggt cctgtatcag ctcttgctat
    26041 tatcgctgct gatgacaatg ctggcttggt tcaagccatt gaaaccaaga acatcaccta
    26101 cttgaccaag ttccctaaaa ttggcaagaa aacagcccag cagatggtgc tggacttgga
    26161 aggcaaggta gtagttgcag gagatggcct tcctgccaag gtcgcagtgc aagcaagtgt
    26221 tgaaaaccaa gaattggaag aagctatgga agccatgttg gctctgggct acaaggcaac
    26281 agagctcaag aaaatcaaga aattctttga aggaacgaca gatacagctg agaactatat
    26341 caagtcggcc cttaaaatgt tggtcaaata ggagcagaga atgacaaaac gttgttcgtg
    26401 ggtcaagatg accaacccac tctacatcgc ctatcatgat gaggagtggg gccagcccct
    26461 ccatgatgac caagcgttgt ttgagttgtt gtgtatggaa acctatcagg caggcctgtc
    26521 ttgggaaacg gtactcaaca aacgccaagc tttccgagaa gcctttcata gctatcaaat
    26581 tcactcagtc gcagagatga ctgacactga attggaagcc atgctggaga atccagctat
    26641 cattcgaaat agagccaaga tttttgctac acgcgctaac gcccaagctt ttctacagtt
    26701 acaggcagag tacggctctt ttgatgccta tctttggtct tttgttgagg ggaaaactgt
    26761 cgttaacgat gttcctgatt atcgccaagc cccagttaaa acacccttat ctgagaaatt
    26821 agccaaagat ctcaaaaaac gaggcttcaa gttcacaggc ccagtcgccg tattgtcttt
    26881 tctacaggct gcagggctag ttgatgacca cgagaatgat tgtgagtgga aaggtcttaa
    26941 atgatgtcta acaaaaataa ggaaattctg atttttgcga ttctctatac agtcctcttt
    27001 gtgtttgatg gcgttaaatt gctggcttct ttaatgccat ctaccattgc aaattatctt
    27061 gtttatgtag ttttagcttt atatggctcc ttcttgttca aggatagatt gatccaataa
    27121 tggaaggaga ttagaaagac taaaagaaaa ttcttctttg gagtcttaac aggatggctc
    27181 tttctcattc tgatgactgt tgtctttgaa tttgtatcag agatgttgaa gcagtttgtg
    27241 ggactagatg gacaaggtct aaatcagtct aatattcaaa gtacctttca agaacaacca
    27301 ctactgatag ctgtttttgc ttgtgtcatt ggacctctga tagaagaatt atttttccgt
    27361 caggtcttat tgcattactt gcaggaacgg ttgccaggtt tactaagcat tattctggta
    27421 ggacttgttt ttgctctgac tcatatgcac agtttggctc tatcagagtg gattggtgca
    27481 gttggttact taggtggagg ccttgccttt tctattattt atgtgaaaga aaaagaaaat
    27541 atctactatc ccctacttgt tcacatgtta agcaacagcc tctccttaat cattttagtt
    27601 atcagtatag taaaatgaaa taagaacagg acagtcaaat cgatttctaa caatgcttta
    27661 gaagtagagg tgtactattc tagtttcaat acactgtaac atgtgatgaa aatgccagta
    27721 atgataccga gaaaaaagct gagaaacttt tcccagcttt atttgttata gtcaaagcga
    27781 atgacttgtt cctgtgcatc tacatgagca tggaccccaa agggtacaat tgctcttgga
    27841 gttgcgtggc cgacattcag attatagaca atcgggatat tgttgtcaat gatatccaat
    27901 agtgcctctt tatagtcgtc atggaaaatt tcatccatag gttttccgac caagagtcca
    27961 ttgatgaccg cgaatatgcc agtgtccttt aaagttagca acatcttttt gaagtcttct
    28021 ggcttaggct cttcttcgct tgtttcgagc aagaggattt tcccttccca gtctgacaag
    28081 tcagggaaaa gtttgtattt ttggcagagt tccgtgctat ctgcgtatcg agagttgtca
    28141 aagatatcgt agagggattc gaggcaacca ccgaggattt tcccctcgaa ctgggcactt
    28201 ccttgcaaca agtcaaaacc tgtatttgta tgactgacac gaggtgttcc cagggccgtg
    28261 ggactaaaat cagttcgttc ctcataccaa acgtcactag ggcggatttc tgaaattctt
    28321 cccgtctcaa tcaattcttt aaagtagtga aggctatagg ctagcatttc tttgtctaat
    28381 tcacaaatgt ctgctaaaaa ggattgacca taaaaagtct tgattcctaa tttatgcaac
    28441 atgaggtggt tcatggttgt atccgagaag ccaagaaaaa ttttttgttt gataaccttt
    28501 tggagttggt cattttcaaa aagataaggt agcaagcgat aggtatcgtc tccaccgatg
    28561 gcacatagga tcatgtcgat gctatcatca gaaaaggcat gaatcaaatc ctctgcacga
    28621 gcttcaggat ggtccttgat aaagtctaat ccttttaacg aatggggcaa aaagatggga
    28681 ttgagtccca gatccttgag acgttggata cccaagtcca cttcgtgttt gacaaaatcc
    28741 tctccgataa tgccactaga taaactaaca ataccaatag tagaaaccat atctcatcct
    28801 cctagaaata gattgagcct attttatcac aaaatataag agaaagctat gtgggatttc
    28861 agaagaatac ttttaatact cttcgaaaat ctcttcaaac cacgtcagtt ttatctgcaa
    28921 cctcaaagct gtgctttgag caacctgcag ctagcttcct agtttgctct ttgattttca
    28981 ttgagtataa ctcaaaaaag taagtgaaaa agcaaccgca cctgcagttg cttttatcgt
    29041 tttcttattc ttgatagcgt gttggtcctg cacttgcact acggatattc aagcgtttct
    29101 tgagatagaa gcgaagggct aggagggctg ctccaataat agctagtggc aatggatcca
    29161 gtactgggtt aaggctagct ggtaggaagc ttgttgcaaa gaagacaagc aaccaaagga
    29221 acatagaagc taggataact agtacagatt tccagaaagg tggacgttga ctgcgatcca
    29281 tatctggtcc atagtattgg taaacaaagt agtacatcaa gtagaaggca aatccaccaa
    29341 ccagtccaac taatagaaga gtaatcaatc catagccgaa agcttgatct gccgcaaaga
    29401 aggttgtgag ggcgctgaca agggcaaaga ggctagtgat gaaaagagct gagtccataa
    29461 tcatcagttt tggatcatca ttttcttttg gatgctcttt ttcgtactgc tctttgacag
    29521 tgaagctatg agcccaatga gttggtgcgc cgtataggga acgggcagtt gtacctttag
    29581 attgctcctc aaggatgtgg ggaatgactt cctcaaaaat agtctggatt tcagcgtctg
    29641 ttttcccatc tttgatgaat tgttgggtag caatgtggac aaactcttgg tttttcttag
    29701 ttaatttttg tagatcaatt tgagacatag gaactcctct tagaaccatt ttttatggat
    29761 gagatagaga gtgagcgaga cactcatagc aaaggcgata aagacgatta accagaaggc
    29821 atttggctct ccgtttaggg ggatttcatt atccttaaag ttcatcccgt aggcagaaaa
    29881 gaccatggtt gggatggaca tgacgatggt cacaagggcc aaggttttca tgatgttgtt
    29941 ctggttgtta gaaatgatag aggcaaaggt ctctgtcata gaatgcaaga cgtttccata
    30001 aatatctgcc atctcgatgg cctgttgggt ttcaatcagg gtgtcttcaa gcaggtcttc
    30061 gtcctcaagg tatttcttga tattgctggt tgaactggtc aatttcttaa tcacgcgctc
    30121 atttgttttg agggaggcct tgaaatagac gatagttttt tccaattcca tgagctcaat
    30181 caattcttca ttacgagttg attgatgcag ttgactttcg atttgttcac tcttgcggtc
    30241 gattgaacga agggctgtta ggtaaagctc tgcattgcga taaagaattt gaaagataaa
    30301 acgtgaacgc atgaaggtat agaaattacg caatcgacgg ttgataaaga catcaaggac
    30361 aggtagtggt tccaaacacg tagtgataat ggtttcctca gtgatgataa taccaagcgg
    30421 gatggttacg tagtaggtgc ggttatttct ttcctccgtg accggcacgt ctacgataat
    30481 cagggtatac tcgtcttcaa tggtaatacg agacatttct tccgcatcga gcggtgctcg
    30541 aaggtcggca atatcaatat cgaaggtgtt ggcgatttcg agtgattcat tttgagtcgg
    30601 attgacgaga ttgatccaag tacccggttc aagcgtatcg atctctttaa attcagttgt
    30661 tgtagagaga aaaacttgtt tcatatccct tatcctttct catttttcag attttttcac
    30721 actgtactat tatactacaa aatcggcctt ttgggtaata gaatctcact ttttttggta
    30781 taatggtaag caataatgga ctagaaagaa caaagatgca agataaaatt gtcattcatg
    30841 gggcgcgtgc ccataattta aaaaatattg atgtggagat tccgcgagac aagttggttg
    30901 tcgtgactgg cttgtcaggt tcagggaagt ccagtctggc ttttgatacc ctctatgcgg
    30961 agggacaacg tcgctatgta gagagtttgt ccgcctacgc tcgtcagttc ttgggaaata
    31021 tggaaaaacc agatgtagat gccattgatg gtctcagccc agctatttcc atcgaccaga
    31081 aaacgactag taaaaatcct cgctcgacgg tgggaacgac gactgaaatc aatgactatc
    31141 tgcgtctcct ctacgcacgt gtggggacgc cttactgtat caacggacat ggagctatca
    31201 atgcttcttc tgtggagcaa atcgttgata aggttttgga gttacctgaa cgccagcgct
    31261 tgcagatctt ggctcctgtc atccgcaaga aaaaaggcca acataagaat gttatcgaga
    31321 aggttcagaa agacggatat gttcgtgtcc gtgtggatgg ggaagtctat gatgtgaccg
    31381 aagtgccaga gttgtctaag agcaagcaac acaatatcga tgtcgtggtt gaccgtattg
    31441 ttatcaagga gggcattcgt agccgtctct ttgattccat tgaagctgcc cttcgtatcg
    31501 cagaaggtta tgtgattatc gacaccatgg acgattcgga gttgctcttc tctgagcatt
    31561 atgcctgtcc agtttgtgga tttactgtcc cagagttaga gcctcgtctc ttctctttca
    31621 atgctccctt tggctcttgt agtgagtgtg acggcttggg gattaagctg gaggtggata
    31681 ctgatttggt agtgccagat gccagcaaaa cgctacgtga gggggctttg gcaccttgga
    31741 atcctatctc atctaactac tatccaaata tgctagagca ggctatgaca gcctttggag
    31801 tggatatgga taagcccttt gaggacttgt cagaagaaga taagaacttg attctctacg
    31861 gctcggatgg caaagaattt cattttcact atgagaatga atttggcggt gtgcgcgata
    31921 ttgacattcc ttttgaggga gtcgtcacta atatcaagcg tcgttaccat gagactaaca
    31981 gtgattacac gcgcactcag atgcgtctct acatgaatga gctgacctgc ggaacctgtc
    32041 aaggctatcg tctcaatgac caggccttgt ctgtccgtgt gggcggccag caagggccac
    32101 atattggaga aatctcagac ctgtctatcg ctgaccactt ggacttggtg agccagttaa
    32161 ctttgtctga aaatgaagcc atcattgctc gtcccattct caaggaaatc aaggatcgtt
    32221 tgaccttcct taataatgtg ggtcttaact atctaaccct gtcacgttca gcaggaaccc
    32281 tttcaggtgg ggaaagtcag cgcattcgtt tggcaaccca gattggttcc aacctatcag
    32341 gtgtcctcta tatcctagac gagccgtcaa tcggtcttca ccagagggac aatgatcgcc
    32401 tgattgccag tctgaaaaag atgcgtgact tgggcaatac tcttatcgtg gtggaacacg
    32461 acgaagatac catgcgtgag gctgattatc tgattgacgt tggtcctggt gccggtgttt
    32521 ttggtggtga gattgttgca gcaggtacac ctaagcaagt ggctcgcaac agcaagtcta
    32581 tcacaggtca gtacttgtca ggcaaacgtg tcatcccagt accagaagag cgccgtgtcg
    32641 gaaatggtcg ttttattgaa gtgataggag cgcgtgagaa caacttgcaa aatgtcacgg
    32701 ctcgctttcc actaggaaaa tttatcgcag tgacaggtgt gtcgggttca gggaaatcga
    32761 ctctaatcaa cagcattctc aaaaaagcca ttgcccagaa gctcaaccgt aattcagaca
    32821 aacctggtaa gtttaagact attacaggga ttgagcatgt cgaccgtttg attgatattg
    32881 accagagccc tatcggacgg acgccgaggt ctaacccagc tacctataca ggagtttttg
    32941 acgatatacg tgaccttttt gctcagacaa atgaagccaa gattcgaggc tacaagaagg
    33001 ggcgcttcag tttcaacgtc aagggaggtc gctgtgaagc ttgctcaggt gacgggatta
    33061 tcaagattga gatgcacttc ttgccagatg tttatgtggc ttgtgaagtt tgccacggaa
    33121 cccgctataa cagtgaaacc ctagaagttc actacaagga aaagaatatt tcgcaggtct
    33181 tggatatgac ggtcaacgat gcggtagaat ttttccaaca cattccgaaa attcaacgca
    33241 aactccagac catcaaggat gtagggctag gctatgtgac cttgggacag ccagctacca
    33301 ctctttctgg gggagaagcc caacgtatga agttagctag cgaactccac aaacgctcga
    33361 caggaaaatc tttctacatt ctggatgagc cgacgacagg gcttcataca gaggacattg
    33421 ctcgcctgct caaggtttta gctcgctttg tagacgatgg caatacagtc ctcgtcatcg
    33481 agcacaatct ggatgttatc aagacggcag accatatcat tgacttggga cctgagggcg
    33541 gtgtcggtgg tggaaccatc atcgtaacag gaactccaga agaagtagct gccaacgaag
    33601 ccagctatac aggacactat ttgaaaggaa agttacatca tgaataaacg cgtacaagca
    33661 tttctagcta aaatgcaaga aaaagaacta gatggtatca tcatcaacaa tcttaaaaac
    33721 gtctattatt tgactggttt ttggggctca aacggaacag tctttatcag ccgtgaccgt
    33781 caggtcttag tgacagactc tcgctatatc atcgcagcta agcaagaaac cagtggtttt
    33841 gagattgtgg ctgatcgtga tgaattggct gtcattgcag gaattgttaa ggacatgggc
    33901 ttgactcgta tcggttttga agatgagatt tcagtgtctt attaccaccg tatgcaggca
    33961 gcttttgcag gtttgaactt gcttccacaa actcagtttg tggaaggtct tcgtatgatt
    34021 aaggatgagg cagagattgc agcgattcgc aaggcttgtt ctatctcaga ccaagctttc
    34081 cgcgatgcgc ttgactttat caaaccagga aaaactgaaa ttgagattgc caacttcctt
    34141 gatttccgca tgcgtgagtt gggagcatct ggcttatctt ttgatacgat cctagctagc
    34201 ggtatcaatt cttctaaacc ccatgcccat ccaatgcaca aaccagtgga gttgggagaa
    34261 gccattacca tggacttcgg ctgtctctat gaccactatg tcagtgatat gacccggact
    34321 atctatctag ggcatgttag cgatgagcag gcagagattt acaatacggt tctaaaagct
    34381 aaccaagcct tgattgacca agctaaggca ggcttaggtt tccgtgactt tgacaaaatc
    34441 cctcgtgata ttatcattga ggcaggttat ggtgactact ttactcacgg cattggccac
    34501 ggtattggtc tggatatcca tgaggaaccc tactttagtc agacttctac agaaactatt
    34561 aagacaggta tggccttgac cgatgaacca ggtatctata tcgaaggcaa atatggcgtt
    34621 cgtatcgagg atgatatcct gattacagag acaggttgtg aattattgac cctagctcca
    34681 aaagagttga tagtcattta gttgtttgtc aagaaaaaag tacgaaaatg acgaaaaaag
    34741 tcaaaaaaaa ttaaaaatga aagttctata atgaagttag ccaccttacc ttagtcaaga
    34801 attagatgtt tcactatgtt tgagtaagtt gatgatttca tttgataaca ggtttgaaac
    34861 tgttaggcta ggtggccaag gctaatcata gccttggttt agctgaaaaa acaggttcaa
    34921 gggttcctgt tgtcaaatga aatgtgattt aaggtataag aaaacacctc tgtgctatac
    34981 ttgttgttca ccacaaacac aaggaaaggc acagagatgc aagaacatta taccccaaaa
    35041 gggaaacatt tgacaataga taaccgtcgc ttgattgagc ggtggaagaa tgaaaataag
    35101 tccaatcgtg aaattgcagg cttgttagga aaggcgcctc aaacgattca tactgaagtc
    35161 aaaagaggta caactttaca acaagtgaga aaagggctat acaaaaaggt ctattctgcc
    35221 gattacgcac aaactgttta ccaattcaat cgaaaacggt cggtgaaaaa gttaatttta
    35281 acaaaggaaa tcagagagaa gatcttacac tatcataagc aaaaattttc gcctgaaatg
    35341 atggttaaca agaagcaagt gaaagttggt atttcaacca tctactactg gtttcataat
    35401 ggtcatttag gattgacgaa ggccgacatg ctttatccca gaaaaaggaa aggtgtcaag
    35461 aagcaagcta gtccgaactt taagccggca ggtaaatcta tcgaagaacg tcctgacgtt
    35521 attaatcttc gcttggaaaa tggtcattat gaaattgata ccgtcctact gactaagata
    35581 aaaaattatt gcctgttagt cttaaccgac cggcggagca gacaccaaat tataaggtta
    35641 attccaaata aaactgctga atctgtcaat caggcgctta cgttactatt aggggagcat
    35701 cgtattctgt ccattactgc agataatggt tcggagttca aacgattgtc tgaggtattt
    35761 cctgaggaac atatctacta cgcacatgct tactcttcat gggagagagg ttcaaatgaa
    35821 aatcataatc gattaattcg gagatggtta cctaaaggaa ccaagaaaac gactccgaaa
    35881 gaagtagctt ttatcgaaaa ttggattaac aactacccta aaaaatgctt ggactacaag
    35941 tcgccaagtg aat
//
LOCUS       DAYYQN010000012        35455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYYQN010000012 DAYYQN010000000
VERSION     DAYYQN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.012"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(70..1704)
                     /locus_tag="ACHBRQ_000561"
     CDS             complement(70..1704)
                     /locus_tag="ACHBRQ_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592278.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1685..2434)
                     /locus_tag="ACHBRQ_000562"
     CDS             complement(1685..2434)
                     /locus_tag="ACHBRQ_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592279.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2613..4067
                     /locus_tag="ACHBRQ_000563"
     CDS             2613..4067
                     /locus_tag="ACHBRQ_000563"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFM9592280.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4157..6775)
                     /gene="alaS"
                     /locus_tag="ACHBRQ_000564"
     CDS             complement(4157..6775)
                     /gene="alaS"
                     /locus_tag="ACHBRQ_000564"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9592281.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAAILKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6797..7282)
                     /locus_tag="ACHBRQ_000565"
     CDS             complement(6797..7282)
                     /locus_tag="ACHBRQ_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9592282.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7557..7796)
                     /locus_tag="ACHBRQ_000566"
     CDS             complement(7557..7796)
                     /locus_tag="ACHBRQ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592283.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8057..9127)
                     /locus_tag="ACHBRQ_000567"
     CDS             complement(8057..9127)
                     /locus_tag="ACHBRQ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9592284.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9124..9897)
                     /locus_tag="ACHBRQ_000568"
     CDS             complement(9124..9897)
                     /locus_tag="ACHBRQ_000568"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592285.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9894..10700)
                     /locus_tag="ACHBRQ_000569"
     CDS             complement(9894..10700)
                     /locus_tag="ACHBRQ_000569"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592286.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10681..11838)
                     /locus_tag="ACHBRQ_000570"
     CDS             complement(10681..11838)
                     /locus_tag="ACHBRQ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592287.1"
                     /translation="MKKSIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRNIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIMGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11999..12904)
                     /gene="murB"
                     /locus_tag="ACHBRQ_000571"
     CDS             complement(11999..12904)
                     /gene="murB"
                     /locus_tag="ACHBRQ_000571"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9592288.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13016..13768)
                     /locus_tag="ACHBRQ_000572"
     CDS             complement(13016..13768)
                     /locus_tag="ACHBRQ_000572"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9592289.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13778..14509)
                     /gene="budA"
                     /locus_tag="ACHBRQ_000573"
     CDS             complement(13778..14509)
                     /gene="budA"
                     /locus_tag="ACHBRQ_000573"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9592290.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIGEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14651..15922
                     /locus_tag="ACHBRQ_000574"
     CDS             14651..15922
                     /locus_tag="ACHBRQ_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9592291.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15942..16757)
                     /locus_tag="ACHBRQ_000575"
     CDS             complement(15942..16757)
                     /locus_tag="ACHBRQ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9592292.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(16914..17567)
                     /gene="phoU"
                     /locus_tag="ACHBRQ_000576"
     CDS             complement(16914..17567)
                     /gene="phoU"
                     /locus_tag="ACHBRQ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9592293.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17579..18337)
                     /gene="pstB"
                     /locus_tag="ACHBRQ_000577"
     CDS             complement(17579..18337)
                     /gene="pstB"
                     /locus_tag="ACHBRQ_000577"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9592294.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEIIALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18350..19153)
                     /gene="pstB"
                     /locus_tag="ACHBRQ_000578"
     CDS             complement(18350..19153)
                     /gene="pstB"
                     /locus_tag="ACHBRQ_000578"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9592295.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19164..20048)
                     /gene="pstA"
                     /locus_tag="ACHBRQ_000579"
     CDS             complement(19164..20048)
                     /gene="pstA"
                     /locus_tag="ACHBRQ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9592296.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20038..20955)
                     /gene="pstC"
                     /locus_tag="ACHBRQ_000580"
     CDS             complement(20038..20955)
                     /gene="pstC"
                     /locus_tag="ACHBRQ_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9592297.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGIWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20955..21833)
                     /gene="pstS"
                     /locus_tag="ACHBRQ_000581"
     CDS             complement(20955..21833)
                     /gene="pstS"
                     /locus_tag="ACHBRQ_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9592298.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22407..23711)
                     /locus_tag="ACHBRQ_000582"
     CDS             complement(22407..23711)
                     /locus_tag="ACHBRQ_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9592299.1"
                     /translation="MQFPEGFVEKYKEILGDEARNFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKSSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23712..24485)
                     /locus_tag="ACHBRQ_000583"
     CDS             complement(23712..24485)
                     /locus_tag="ACHBRQ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9592300.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYLEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24475..24753)
                     /locus_tag="ACHBRQ_000584"
     CDS             complement(24475..24753)
                     /locus_tag="ACHBRQ_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9592301.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24753..25154)
                     /locus_tag="ACHBRQ_000585"
     CDS             complement(24753..25154)
                     /locus_tag="ACHBRQ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9592302.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25364..25981)
                     /locus_tag="ACHBRQ_000586"
     CDS             complement(25364..25981)
                     /locus_tag="ACHBRQ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9592303.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(25971..26744)
                     /locus_tag="ACHBRQ_000587"
     CDS             complement(25971..26744)
                     /locus_tag="ACHBRQ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9592304.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRGIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26755..27492)
                     /locus_tag="ACHBRQ_000588"
     CDS             complement(26755..27492)
                     /locus_tag="ACHBRQ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9592305.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSVVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27497..28627)
                     /gene="hemW"
                     /locus_tag="ACHBRQ_000589"
     CDS             complement(27497..28627)
                     /gene="hemW"
                     /locus_tag="ACHBRQ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9592306.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28711..29100)
                     /locus_tag="ACHBRQ_000590"
     CDS             complement(28711..29100)
                     /locus_tag="ACHBRQ_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9592307.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29073..29499)
                     /locus_tag="ACHBRQ_000591"
                     /pseudo
     CDS             complement(29073..29499)
                     /locus_tag="ACHBRQ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            complement(29500..30288)
                     /gene="lgt"
                     /locus_tag="ACHBRQ_000592"
     CDS             complement(29500..30288)
                     /gene="lgt"
                     /locus_tag="ACHBRQ_000592"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9592308.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGVLVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30281..31216)
                     /gene="hprK"
                     /locus_tag="ACHBRQ_000593"
     CDS             complement(30281..31216)
                     /gene="hprK"
                     /locus_tag="ACHBRQ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9592309.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31346..31522)
                     /gene="rpsU"
                     /locus_tag="ACHBRQ_000594"
     CDS             complement(31346..31522)
                     /gene="rpsU"
                     /locus_tag="ACHBRQ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9592310.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31662..32369)
                     /locus_tag="ACHBRQ_000595"
     CDS             complement(31662..32369)
                     /locus_tag="ACHBRQ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9592311.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32520..33548
                     /gene="queA"
                     /locus_tag="ACHBRQ_000596"
     CDS             32520..33548
                     /gene="queA"
                     /locus_tag="ACHBRQ_000596"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9592312.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(34209..35234)
                     /locus_tag="ACHBRQ_000597"
                     /pseudo
     CDS             complement(34209..35234)
                     /locus_tag="ACHBRQ_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT        10564 a         8106 c         6736 g        10049 t
ORIGIN      
        1 acaactacat tctaacaaac tataaaagcg tttgtcgaat tgaatttatc aagcaagcaa
       61 ccaactatct catctttttt ctatttctgc caatatgcgt gacaggtaat aatgatagcc
      121 aaaaatagca agagcaagca agacgataag agctcctact cccaagttga tggcaaggat
      181 aggggagaga gactgaacca agaatatgct cccaattaca agggccatca ggattgcact
      241 ataaataaac aataaaacta tggcgactat gccatttgaa cgattcacca ggtccgtaat
      301 gctactccaa ttggttgaca gatttttaac gtccttaaag taatggtggc aagaaaggat
      361 gacactggca atgatccaga ctacaagaag gtaaatcatc gaaaggatgg acaagcctag
      421 atatagagaa agaccgagca aagtcagaac tggtaaaaag gactgaacag caaagataat
      481 ccaaaatttc actttcacat aacgagcaaa gtcaaagggt aaactcttca gaaaatcaac
      541 attttccctc tccaaggaca aggcaattga atgcaggctg gtgatattgt tattgacaac
      601 tgctataaac actcctacaa aaaacaaggg taaccagtat ggaggatgaa tgtctggaac
      661 tatctgagaa tctcggattt tggaaatcag actgatcatc atgagataag gaaggaaagc
      721 acttgtaaaa agcactgtaa tcacaccagt cccctgtccc aagagggtga ggtggtagcg
      781 taaaaccata cggaaaaagc cctttttagt agttgaaatc ctctccttgc tgcgacgttc
      841 ttttttgacc ttctcctcac tgttaagcag gatcacgtca taaaaacgag gaaggacctt
      901 ctttttggtc agataaagca ggaagagagt tagtcctatc caagcgagca gacccactaa
      961 ggcttctgtc gaaaaaggct ccactgctat tttgtaaaag atatgaagag gataaaggag
     1021 aaatggaatg tctctaactt tgtcaacaat acttccaaaa gtcgactgaa gaaagaagat
     1081 aaatattaaa ggtatgagaa ctcctatccc aatcatcaca ttcgaaaaaa tagactgata
     1141 ctttctgaag accctagtct gagccaagaa atgtactgcc actaccgtca ctaaagtaac
     1201 agagacaaat aataaggtca aggacagtag catcaaaggc aaacccagcc aaagagaagg
     1261 agctagacta atatagaggg ctagaaaata agctaggatt ggtacaattc cagttagagc
     1321 tggcaagagg acagacagac ctttggccat tataatctct gattctttaa aggcataggg
     1381 cctataggag gccaggtcct tactctcata aaagacattg taaaaggcag tgaaagaagt
     1441 tgaaaaggcc agcaccagta aaatagcaac catcgagcta aaataaatag gcatttcctc
     1501 aaaaggaaaa tgaatggcta tattactaaa acagatgagc atcaagagac tggaaaaaat
     1561 gtaagaactt aggactctag cagaaacatt tacttttttc ccaggattct tagcctgctt
     1621 ctttcgtaga ttagccagat tagcttcttg agatgaatag aggatattga tatcaactaa
     1681 ttttttaatg accttgagac gcatccgcaa cctcctcttt tctaccagca agactaaggt
     1741 agatactttc caaaaactgg tctgggtggt ctttcctcaa gttctctaca ctaccacaat
     1801 agatcaaatg cccctttttc aaaatagcaa tccgatcaca gacttgctcg gctacctcaa
     1861 ggacatgagt tgaaaacaag accgtcttgc ctttttgtgc atgttccttc atcatttgct
     1921 tcaaatcaaa ggcggcctgg ggatccaaac cagtcaaggg ttcgtccaaa acccaaatat
     1981 caggagcaga caagagtgcc ccaatgacaa agactttctg acgcattccg tgagaaagag
     2041 tttcaataac ctgatagcgg ttttcagcaa aatcaaaaac gttcaatagc ctagctagac
     2101 tagtctccaa gtcagagcta gtcagatcat aggatgaagc gatcaattcc caaaattcat
     2161 tggccgttaa gcgtaaaaat aagtcaggcg agtctgctac gtagccaatc tttcgtttga
     2221 tcgccaagcg attttccgat aactcctgac cgtctaccaa aatacgacca ctgctgggtg
     2281 aaatgatact gactagggat tttatggtgg tcgattttcc agccccatta tgaccaatca
     2341 agcccataat ctctccattt tcaatctgca aattgagatt actcaaggcc tctttatcac
     2401 catacaactt actaacattt tgaaattcaa tcatgggaca actcctatct ttatctgtat
     2461 tatacatcgc tatactagca ctttgataca aaaaaatcaa tactttattt taagtagtta
     2521 aaatagtttc cttctatctt acgcaaacgt ttgcgccgag agatacttta tgatagaata
     2581 aagaacaaga ttgacaagta agaggaaaca tcatgcaaaa tcaaacactc atgcaatact
     2641 ttgaatggta tctgccccac gacggtcaac actggacgcg tctggctgaa aatgctccac
     2701 acctagctca tctggggatc agtcacgtct ggatgccacc agccttcaag gcaaccaacg
     2761 aaaaagatgt cggctatggg gtctatgact tatttgactt aggagagttc aaccaaaaag
     2821 ggactgtccg caccaagtat ggtttcaaag aagactatct tcaagccatt caagccctta
     2881 aagaacaggg aattcaacct atggccgatg tagttctcaa ccacaaggct gctgccgata
     2941 acagggaagc ctttcaggtt atcgaagttg atcctgtaga ccgtacagtt gaacttggag
     3001 aacccttcac catcaatggc tggactagtt ttaccttcga tggtcgccaa gatacctata
     3061 atggcttcca ctggcattgg taccacttca ccggtacaga ctacgatgcc aaacgcagta
     3121 aatctgggat ttatctgatc caaggggaca acaagggctg ggctaacgag gaattggtcg
     3181 ataacgaaaa cggaaactac gactacctca tgtatgccga cctagacttt aaacatcctg
     3241 aagtcatcca aaacatctat gactgggctg attggttcat ggaaacgact ggtgtagctg
     3301 gtttccgttt ggatgccgtt aagcatattg actctttctt tatgcgcaac ttcatccgcg
     3361 atatgaagga aaaatacggt gacgatttct atgtttttgg tgaattttgg aacccagaca
     3421 aggaagccaa tctggactat ctcgaaaaaa cggaagaaca ctttgacctt gtcgatgttc
     3481 gtctccacca gaatctcttt gaagccagtc aagctggcgc aaactatgac cttcgtggca
     3541 ttttcacaga tagcctggtt gaactcaagc ctgacaaggc tgtgactttt gtcgacaacc
     3601 acgataccca acgaggacaa gcccttgagt ctaccgttga agaatggttc aagccagcag
     3661 cctatgccct cattttgtta cgccaagacg gccttccatg tgtcttttac ggagactact
     3721 atgggatttc aggccagtat gctcaagaag atttcaaaga aatccttgac cgcctcctag
     3781 ccatccgaaa agacttggcc tatggagaac aaaatgacta ctttgaccat gctaactgta
     3841 tcggttgggt acgttcaggt gctgaaaatc aatccccaat cgcagtcctt atctcaaatg
     3901 accaagaaaa cagcaagtca atgtttgtcg gtcaagaatg gactaatcaa acctttgtag
     3961 atttacttgg taaccaccaa ggtcaagtta caattgatga ggaaggttat ggacaattcc
     4021 ctgtctcagc tagatccgta agtgtctggg cagtcaatac catctaatag ctcataataa
     4081 ccaagctagg tccaagcgga tttggctttt ttgtattcac aaaaagacct acccaaatgg
     4141 atagatcttt acttgattac aatttacctg ctactgcatc caacaattct tggatcttag
     4201 cttggttgct tcctcctgcc atggccatgt ctggtttacc accaccacgt ccatcgatga
     4261 ttggtgctaa ttctttgaca aggtttcctg catgaaggtc ttttgtcttg cttgctacaa
     4321 ggacattgac tttgtcaccg atagcggcaa ctaggacaag aagatcagag tagtcttttt
     4381 gtttccagtt atctgcaaaa gtacgaaggg caccggcatc ggatacagac acttgactag
     4441 caatgtaacg atgaccgttg acttccttag catctttgaa gatatcgcct gcggctgcag
     4501 ctgcggcttt ttctttcaac tcagcatttt ctttttgaag ttgacgaagt tgttcttgaa
     4561 gtccttctac cttgtgaggt acttccttga cttgaggtgc tttcaagatt gctgcgacag
     4621 ctttaagagc atcctcttgt tcacgatagg cttcaaaggc ttccttacca gtcactgcca
     4681 agatacggcg agttcctgaa ccgattcctt cttctttgac aattttgaag agaccaatct
     4741 cagaagtgtt gccaacatga gtaccaccac aaagttcaat agagtagtca ccgatagtca
     4801 cgacacgaac ttccttgccg tatttctcac caaagagggc catagctccc atttctttag
     4861 cagtgtcaat atccgtttca actgtcttaa cttcaagagc ttcccagatt ttttcgttga
     4921 cttgctgttc aatcgcacgc aattcttcag cagttacagc ttggaagtgg gtaaagtcaa
     4981 agcgaaggaa ttcaacttcg ttaagagatc ctgcctgtgt tgcgtggttt ccaaggatat
     5041 tgtgaagggc agcgtgaagc aaatgagtcg cagtgtggtt tttcatgaca cggtgacggc
     5101 gattgctatc aattgccaag gtatattctt ggttcaaggc aagcggtgca aggacttcaa
     5161 ctgtatgaag ggcttgacca tttggggctt tctgaacatt ggtcacagta gccacaacct
     5221 tacctgactc atccaagatt tgtccgtagt cagctacctg tccacccatt tcagcataaa
     5281 atgacgtttc cgcaaagata agagaggcag ttccttctga aacagcttct acttctgcat
     5341 tgtccgccac gatagctacc aatttagaag acaattggct agcattgtag ttgaagacac
     5401 tttctacagt gatgttttga agagtttcat tttgcatacc cattgagcca cccttgacag
     5461 ctgaggcacg cgcgcgttct tgctgttctt tcatggctgc ttcaaaacct tcacggtcta
     5521 cagtcatacc agcttcttca gcgatttctt cagtcaattc aactgggaac ccataagtat
     5581 catagagttt gaagacatct gaaccagcga taacagattg acctttttct ttcaagtctg
     5641 ctacaatgcc ttgggcaaag tgttgacctg agtgaagggt acgggcaaat gattcttctt
     5701 cgctcttaac gattttctca ataaagtcac gtttctcaag cacttctggg tagtagcttt
     5761 ccatgatttt tccaacagtt ggaaccaatt tgtaaaggaa aggttcgttg atacccaatt
     5821 tttgaccatg catagaagca cgacggagaa gacgacgaag aacataacca cgaccttcat
     5881 ttcctggaag ggcaccatca ccgatagcaa atgaaagaga acgaatgtgg tctgcgataa
     5941 ccttgaagct catgttgtcg ccatcttggt cataaacctt accagacaat ttctcgactt
     6001 cacggataat cggcatgaag aggtccgttt caaagttggt cttagcccct tggataacag
     6061 ccaccaaacg ctccaaacca gcgcccgtat caatgttctt atgtggcaat tccttgtatt
     6121 cgctacgagg aacagcaggg tctgcgttaa attgtgacaa aacgatgttc cagatttcaa
     6181 tataacggtc gttttcaata tcttctgcaa gcaggcgaag accgatattt tctgggtcaa
     6241 aggcttcccc acggtcaaag aagatttctg tatctggtcc agaaggtccc gcaccgattt
     6301 cccagaagtt gtcctcaatt ggaatcaagt gacttggatc cactcccact tcaatccagc
     6361 ggttgtaaga atctttatcg tctggatagt aggtcatgta aagtttttca gcagggaaat
     6421 caaaccattc agggcttgtc aaaagctcat aagcccaagt gatagcttcg tcacggaagt
     6481 aatccccgat agagaagttc cccagcattt caaacatggt atggtgacgc gcagtcttcc
     6541 ctacgttttc gatgtcgttg gtacggatag ccttttgggc attggtaata cgtggatttt
     6601 cagggataat ggtcccgtca aagtatttct taagggttgc taccccagag ttgatccaca
     6661 aaagagttgg gtcatttaca ggaaccaaac ttactgatgg ttctactgag tgagctttgg
     6721 tcgcccagaa atcaagccac atttggcgta cttgtgcact agatagttgt ttcatattgt
     6781 ctccttattt acttgtttaa tgtgattggc tttccagcat ttccacatag tcaatcgcga
     6841 cacagaggga aatgactagg tctgcataag cgtcctcaag aaccgttacg gtataggtag
     6901 aggtcagatg gaagagttcc ttcttaattt ccgcaatcag ctgatcgcga tcatccagca
     6961 atttgaaatt caaatcccag atattgccct cgatacgaag acctagatta tcaaactcat
     7021 acttatctcg ccagaaggtc aacttcttac gaatgacaaa actcgagcca tcccgaagct
     7081 gaatctcaaa acgaggaagc aaggtcaaga tttctttact aatctcactg acttgttcac
     7141 cagccgcatc atagatggta aaggttttag gaatcttaaa aaatgatccc tccacctgat
     7201 aggcaatttc tcccctgtca ttcttgatag cgaagcgttc gcctccaaga cgaaactttt
     7261 gtttgacaag aaatgttttc atcaacacct ccaaaaatca aaagacaagc tcatatcacg
     7321 aagggcgaaa aaccgcggta ccaccttcat tcaatgaact tgtcattctc ttgttcttat
     7381 gcaattgtat gattgagtag catgacttcc tagcttagat ggctcgcagc accgccattt
     7441 ctctggacta agacaagtga aaatcaattc tcaactttct tattataacg tttttttaag
     7501 cttgcgtcaa ctggaaatga tctccgttga attagaccaa ttccctacat ctctgattac
     7561 tttttcagga tatatttttt cttactgccg tttttctttt tatcccaaat tttcatatta
     7621 ctaaacacag ctactagaat atttccaaat ataaaggtgc ctatcaccca atatatggac
     7681 tcagttgtta ggtattgtcg atccaagcca tcctttaaat ggaatagtat aacagtttgg
     7741 ttaacaatca taaaggttgg ccagaaactt tttttgaaaa aagtagacat tttcattatt
     7801 tgttgccgct ttctgtaagg ttaatactca ataaaaatca aaaagcaaac taggaagcta
     7861 gcctcaagct gtacttgagt acggcaaggc aacgctgacg tggtttgaag agtataggct
     7921 tagtatacta ctaggcaagc aaataagcaa ataaacaaat aaacaactag aatagaaaaa
     7981 gatagggctc taaaaactga cttctattcc ttaaaaacga accagcttga ctgattcgtc
     8041 ttcttacgtt tatctcctac ttccgataca ttttaaactg taggaagagg tcgctatatt
     8101 tccctgtcca tttatggtca aatttctcat aaacttctag gtgtttcatg gtttcaacat
     8161 cgggatagaa ggccttatct tcctttgttt cctctgggag caattccttc gctggtaggt
     8221 ttggtgttga atagccgaca tactccgcat tttggagagc attttcaggt ttcaacataa
     8281 agttgataaa ggcataggct gagttttggt ttttaactgt tttgggaatg accatattgt
     8341 caaaccaaag attgctggcc tctgtcggta ccacataacg tagattttca tttttttcta
     8401 acatttggct ggcttcacca gagaaggtca cgccgattgc aacattattc tgaatcatat
     8461 agcccttcat ctcgtccgca acgatagcct tgatatttgg agtcagtttg tagagcttat
     8521 ccactgtctc ttccaactgc tgcagatcct tggagttgag gctgtagccg agggaattga
     8581 gtcctagtcc cagcacctca cgcgccccat caaagagcat gatagaattc ttatactccg
     8641 gcttccaaag gtcatcccaa tgctcaggcg cttcatctac catggtttcg ttgtagacaa
     8701 ttcctaaggt tccccagaag taagggatgg agaatttatt acctgggtca aaggactggt
     8761 tgagaaactc tggtccgata ttttcgattc ctttaatttt tgaataatca agcggaacca
     8821 agaggtcttc gtccttcatc ttgttaatca tgtattcact tggaatggca atatcgtagg
     8881 tcgttccacc ctgctttatc ttagtgtaca tggcttcgtt ggagtcaaaa gtctcgtact
     8941 gaacttgaat tcctgtttct tctgtaaact gagtcaagag ttcaggatcg atatagtctc
     9001 cccagttata gataaccaat ttttgactat ctcgactatt gattttacta tctaaatgag
     9061 tcgcaattcc ccacaagaca aggataatcg ctgcaattcc tgctaaaaat gaatagattt
     9121 ttttcatgct tgctcctcct tctcacgaga gataaagtaa taacctacaa ctaggataat
     9181 actaaagaga aagactagag cagacagggc attgatttct aaggaaatcc ccttgcgagc
     9241 acgagagtaa atctcgactg atagggttga aaagccattt cctgttacaa agaaggtcac
     9301 ggcaaagtca tctaacgaat aggtgaaggc catgaaataa ccagtaatga tagacggagt
     9361 caggtaagga agcatgattt ccttgaacat ctgaaattga ctagctccca agtcataggc
     9421 cgcatgaatc atgtcgccat tcatttcctt gagtcgaggc aagaccatca agaccacgat
     9481 aggaatggag aaggccacgt gactagatag aacggtcaaa aagccaagtg aaaacttgag
     9541 ttgggtaaag agaatcaaga agctagcacc aatcataacg tcaggcgcaa ccatgaggat
     9601 attattgagt gatagaaagg cttcttggta tttcttacga gactggtaga tgtaaatggc
     9661 accaaaagtc ccgataatgg tcgctatcaa ggctgatagg aaggccaaga aaaatgtctg
     9721 agccaaaatc agcatgagtc tcccatctcc aaacatggtt tcaaagtgag tccagctaaa
     9781 acctgtaaag ctattcatat catcaccagc attaaaggca tagccaatca agtaaaagat
     9841 aggcaggtag aggaccagaa agaccagtcc cagataaagg ttggcaaatt ttttcatcgt
     9901 tctctccttt ccttagtcac ccacatggtg atgaacatgg tcaggatgag aatcacaccg
     9961 atagttgaac ccataccata gttgtcattg gttagaaaat tctgctcaat agccgtcccc
    10021 aaggtgataa cgcggttccc accaatcaaa cgggtcagca tgaagagact caaacttggg
    10081 ataaagaccg actgaacccc acttcgcaca ccgttcatag atagagggaa gatgacatga
    10141 cggaaggtct cccacttagt tgcaccaagg tcgtaactag cattgatgag attattatcc
    10201 atatcgtcta agacattgaa aatcggcaaa atcataaagg ggagctcgat gtagcttgcg
    10261 acaaagataa atgagaaatc agtaaatagc aactgctgtg aaccaattcc gataaattcc
    10321 aagaattggt taatagagcc attctgacca aaaatcccga taaaggcata agccttaaga
    10381 agaaggttga tccaagttgg cagaataatc agcataatcc agagttgacg gtgtttgaga
    10441 cgggtcaaaa agagggccgt cggataactg ataagcagtg tcacaaaggt cacaatgcct
    10501 gcataaagca ctgagttgaa actcatttta agataggtca agttttgtga cgcaaagtaa
    10561 gatttgtaat tttctaaact gaactggcct tcgatgttga aaaaggattg accgaaaatc
    10621 aagaccaagg gtgccaatac aaagagggca atccaaagca tgtagggtac tacaaagagt
    10681 ttagagcttg ttttcttcat ctctttcctc ctcgattgca ttgatcaaac ctgcttcttg
    10741 ctcttcgatt tctacgtact cctcaatacg agcatcgaac tcttcttcgg tttcattgag
    10801 acgcatgatg tggatgtctt ctggttcaaa gtccagaccg atttcctcac ccatgatggc
    10861 cttacgggtt gagtggatca tccattcatt tccaagttcg tcataggcga taatttcata
    10921 atgcacccca cggaagagct gggtatcgac cttaacttga agcttgcctt cttcaggaag
    10981 ggtaatgcgc aagtcctctg gacgaataac gacctcaaca ggttcatttg gcttcatccc
    11041 accatcaacc gcttcaaagc gtttgccgtt aaattcgacc aagtagtcct caatcatggt
    11101 acctggcaag atgtttgact ccccgataaa ggtggcaaca aagtggttga ttggctcatc
    11161 gtagatgtcc acaggggttc cagactggac aatctcgcca tcattcataa cgaaaatcca
    11221 gtcactcatg gcaagagctt cttcctgatc gtgagtgaca aagacaaagg taatgcccaa
    11281 tcgttgttgc agttcacgca gttcgtactg catgtctgtt cttaatttca agtccagcgc
    11341 tgacaagggc tcatccaaca aaaccacacg gggttggttg atgatagcac gggcgatagc
    11401 cacacgctga cgttgtcctc cagaaagttt gcggatggaa cgtttttcat aaccttccaa
    11461 ctgaaccatc ttgagaactt ccgctacacg ttgctcgatt tctttcttat caatgttacg
    11521 caagcgaagt ggaaaggcaa cattttcaaa cacattcata tgtgggaaca aggcatagga
    11581 ttggaagaca gtatgtacgt cgcgcttgtt ggttggaata tcattgatac gaacaccgtc
    11641 tagcatgata tctcctgtcg tcgcatccag taaacctgca ataatgttta ggatagttga
    11701 tttccccgaa ccagatgcac ctagaagggt gtagaatttc ccttcttcca actcaaagtt
    11761 gatgtctttg agaaccttgg tgttgctgtc ttcaaaaact ttagagacgt ttttgaattc
    11821 gataattgat tttttcaatt ggcataaatt ccttcttttt catagattaa ccgatcgggg
    11881 ctctgtcagg tcgccactac ctcttgcagg gagtaaaacc acctgcatac atcttcgcta
    11941 ccaataggct ttcacccccc tttccatgac atagcagcag cttttcaaat acagcttcct
    12001 acttgctttc acccaagatc cggacttctc tttcaagcgt aatacctgag tgttccttga
    12061 ctttttcgat aaccgattgt atcaagtcct cgtagtcttt ggccgttcca tctgcgacat
    12121 tgatcataaa tcctgcatgc ttttctgaca cttctacgcc accgatacga tagcctttca
    12181 agccagcttc tgaaattaac tgacctgcaa aatgcccgac tggacgctta aagaccgagc
    12241 cacaagatgg gtattccaaa ggttgcttga gttcacgtag gtgcgtcaag cggtccattt
    12301 cctgcttgat aacctgatgg gttcctgggg ctagggcaaa tttaactgac aagacaactg
    12361 caccagactc ctgaatagct gaatggcggt aaccaaaagc caagtcttta gcagacaggg
    12421 tttcgatttc tccatccttg gtcaagacct tacaagactg caagatgtga gcaatctcgc
    12481 caccataggc acccgcattc ataaagacag caccgccaac gcttcctgga ataccacaag
    12541 caaactcaaa gccagtcaaa ctatgacgga gggcaatgcg agttgtttca atcaagttag
    12601 ccccagcttc tgcttcaatg gtatagccat caacagaaac gttattgagc ttgtcacaca
    12661 agatgacaaa tccacgaatc ccaccatcac gaacgatgat attgcttgca ttgccaagaa
    12721 ccatccaagg gatattttct tggttggcaa atttcacaac gcgagccaac tcaaaacgat
    12781 ttcgtggaaa gaccaaataa tcagcctctc cacctacttt tgtataacta tagctatgca
    12841 agggttcctt aaaacggata tcaattcctt ctaagatttc aagcattttt tctcttacag
    12901 acatgtcact cttcctttta caaaattcat tccattatac catttttaga gacatttgac
    12961 gaccataaaa ataccttgtt tggattttgc ataagaaaaa gaggttcccc cctttttatg
    13021 attttttaca aaagatttcc ttggttccat aggcaaccag aacgagctcc agtgctagaa
    13081 tcacttcaac caagactgga tttgtcaacc agcctacttg gaaaagagat ggtgccagat
    13141 caaagaaggc atgcaagcca taggctgcta ggagataaat ccatttcttc tggcgaacag
    13201 cttggtaaac ccaaactgtc aaaagtaatt ggaaaccaag cgccaagatt cgctcaaaac
    13261 caagcaaata aatctgccag accgaaagtg actgaatggt ttttaacata ttttcagaca
    13321 gtaattgcat aacctgtgga ttctgagttt gaactgccga aagaacgatg taaagattga
    13381 gtaaactaat aaggcctaga aaaatcaact ccaagccacc atgccctagg ccataggcca
    13441 aggcatctgc cttttccaaa cttctctttt tctccaacca tttgaagaaa acaagacgag
    13501 ccgtttcctc aaaaaatgct gccatggcta ggccatagat aatatagata agcggatgat
    13561 cttgcaagag ggcaatacta ccgtcttttt gaggatgcaa aatcaagata tgcaccagtt
    13621 tttccaaaat ctgcgaagag acaaagaaag cgactgcacc caagcccaag acagctagat
    13681 taatctggta tttctttttg gcaaaccaaa tgcttcctat cagaaaagct agcaacagca
    13741 acatggtaat aatgatatga atggtcattt tcttctccta ttctgccttt tcaatatctt
    13801 ttttcatctc gtcaacattg aacttagcaa acaagtattg acggtcttgg actgggaaac
    13861 gttggtccaa ctggtcaact gctcccacct cgataatgcc ttccttgatg acaaaatcca
    13921 tgacatgtcc accgaaggtc aaatcatctg atatgaagtg cagatggtag cctgccacac
    13981 tgaccccatg gaaaatctca ggcgtccaga aaccaacaat agtccccgca acattgtcac
    14041 gactatattc cggttgatgg gttgcgacat cagcaaactt ggtatcgggt gttgacttag
    14101 gaatcatacg cacatgcata tgtgaaaatt ccccccgaat cttgatagag cggaaaagat
    14161 tttccccatc ataataagac tcaattcgtt ctttcaattc cttgtctgtc atctcaaagc
    14221 gctggcggaa aatgacctct gcctgatgcg gtaccactgc agcgtaagga ataagggcat
    14281 ctggtgacac ttccccaatt tctggctggt ctcctgaccc tttggcctga taagccttgc
    14341 catccaagac aatcaattcc ccatcaatgg aatccaaggt tcccaaacca aggtcaccat
    14401 gttctagcaa ttctcctact gtcatagtac caccataaag gccagccatc aaggctccaa
    14461 gggtattgta ttgaaataat ttcactggtt cctgcacgtt cttatccatt cactttcttg
    14521 tctgttattc tataaatctt actcctaagt ataccacatt tgcccctaga tgtgaacgag
    14581 agaaacgctc tagacattgc caagaaggaa aaaaaaaggt acaatgtaac aaaatcaagg
    14641 gaggtctgga atgaagaaac aaagcaagta caaagaggtc gtttcctatc tgaaaaatgg
    14701 tatcgagtct ggacgatttc cgacgggtag tcgcctgcct tctatccgtc aactgagcct
    14761 tgactttcac tgcagcaagg acaccattca acgagccctg ctggaattac ggcacgaaca
    14821 atacctctat gccaagcctc agagtggcta ctatgtatta gaacaagggc aacatcaaga
    14881 cctagaaatc gaggttaccg acgaacatgc cagtgcctat gacgatttcc gactctgtgt
    14941 caatgaaacc ttgattggcc gagaaaacta cctcttcaac tactatgaca atcaagaagg
    15001 attagaagac ctaagacagt ccattcacaa actcctcttt gagcaagctc tctactgcaa
    15061 ggctaaccaa ctagtactga cttctggaac ccaacaagcc ttatttatcc tctctcaaat
    15121 atcctttcct agacaagcca aggaaatctt ggtggaacag ccaacctacc atcggatgaa
    15181 tcgcctcttg attgcacagg ggctggacta tcaaacgatt gaacgaggca ttgatgggat
    15241 tgacttggag gagctggaag gccacttcaa aacaggaaaa attaaatttt tctacaccat
    15301 tcctagattt cactatcctc tgggacattc ctattctgag caagacaaac gatctattct
    15361 taacttagct gccaagtatg atgtctatat cgtagaggac gattatctgg gtgatttgga
    15421 ctccaagaag ggccaaacct tccactatct tgatacagag gagcatgtca tttatatcaa
    15481 gtccttctcg accagccttt ttcctgccct tcgtattaca gcactcattc ttccaaatgc
    15541 tatcaaagaa gcatttgtgg cctacaaaaa tatcctagac tacgacagca acctcattat
    15601 gcaaaaggcc ctgtcactct atattgacag tcaattgttt gaaaaaaatc gtttggctcg
    15661 cttgaccaat catgaatctt accaaaaaca aatcgaggaa aggataacta aaacaccttg
    15721 tccccttcct cattatcccc tacacgatgg tttattgcta gacctgagac agtatcctaa
    15781 aatcgccagt ctcaaacaca gtcaaccggg cttggacttc tttgaagagg cctatttaag
    15841 tacctgtcct tatcaatttg ccaaggtgtc cctagacaat ctggaaaatg ttttaaacta
    15901 tttaaaagca gaattggaat gacatccaac tctgcttttt cttattcttc aacttcagtt
    15961 agacttgctg ctttttcaag atagctttgg taaattcctt catccttaag tttttggatg
    16021 accttgtcta ccacttcttt caaatcatca ctattttttc taagagcaac ggcattggcg
    16081 tcgccgtcct tcatcttcaa gctgacagtt gcgacagcta agccagcgtt tttagcagca
    16141 taactaagtg caacaggctc atccatatga acagcatcta cttttccagc ctgcaattca
    16201 ttgactgctt cacccatatt agttagggaa gttaattgag cttttggcaa ttgttccttg
    16261 accattgatt ctggaacagt ccctttttgg gctgcaatat tagcactttc taggctagtt
    16321 aaatccttgt atttttccac atcagcctta cgaaccaaga aactaatctt gttttcatag
    16381 tatgggattg aaaaatcaaa gacttctttt ctctcgtcag tagcactaat tcctgcaact
    16441 gctaggtcag ccttaccagt ttgaagactg gtcaaaacat tgtcaaaact catgcttgag
    16501 atttccaact taaccccaag ttcatcagcg atagcctgag ccatgtcgat gtctgcaccg
    16561 actacctggt tctttccatc aaccaatgat tgaaattcaa agggtgcata gtcaggactg
    16621 gttgccacaa ctaatttccc cttttgctta atggcctcaa cagctgactg agaaccatta
    16681 gaacctgact ggcaagctac taagaagaaa cttgcaagaa aattacataa aacaaatatc
    16741 catttttttg atttcataaa taaacaacct ctctgttaat tttgtataat tataacgata
    16801 tccaagttac ttgtcaagtg ttttttaaat ttttatctca aaaatatttt ttcgttcaaa
    16861 aaaaggagcc atcagttgat ttcaagctcc tttttataca gaattaaact attttatagt
    16921 tcgacaatct tacctgtttc aaagtagaca acccattcac agatattttt agcatagtca
    16981 ccgatacgct ccaagtagga aataacttgg aaataatcac gacccgtaac aatggcttct
    17041 ggatttttct taatctcttc agtcgcaagg tcacggatag tttcaaaata gtggttaatt
    17101 tgctcatcca tggaggccac ccggtatgcg tcgtcaacag aaccattaag ataaagatca
    17161 agtgctgctt ccacaacgct tttaacttca cgtcccattt ttttaatttc ttcctctaca
    17221 gctgggatgc gttcttcccc cttcatacgg atggttgcct gggcaatggc tacagcgtga
    17281 tcccccatac gctccacatc tgatacagcc ttaaggacag tcaagactgt acgcaaatct
    17341 tgagagactg gttgttggag tgcgatcatt tcaaatgatt tcttttccag tttcacttcg
    17401 tattcattta cttctgcatc atcttcgatg acctcttttg ccaggtcacg gtcatgcgtg
    17461 acaaaagcac gtaccgtacg attgatttgt gagagcactt cttgtcccat agcgtagaac
    17521 tggttatgta atttctctaa atcttcttca aattgagatc gtaacatctt tcatctcctt
    17581 atccaaattt tcctgtaata tagtcttccg tttccttgtg ttggggatca aggaacatct
    17641 gcttggtatc attaaattca atcaaatctc catctaggaa aaatcctgtc ttatcagaga
    17701 tacgtgaagc ttgctgcatg gaacgggtta ccagaagcat ggtgtacttg tcttttagac
    17761 catacaaggt ttcctcaatt ttaccagctg aaatcggatc caaagccgaa gttggctcat
    17821 ccaagaggat gattttagga ctagttgcca agacacgggc cacgcagaca cgctgctgtt
    17881 gaccacctga caatccaata gctgaatcat atagacgatc cttgacctca tcccagatag
    17941 aggcaccttg caaggctttt tctacggctt catccagaac ctgcttatcc ttaattccat
    18001 tgatacgaag cccgtagaca acattctcat agatagtcat agggaaagga ttaggttgtt
    18061 ggaaaaccat tccgatttcc ttacgtaatt caaccgtatc tgtacgcgga ctgtagatgt
    18121 tgtgaccatt gtacaccacg gatccagttg tggtcacctc tggattgaga tctcccatgc
    18181 ggttgagaga cttgaggagg gttgacttcc ctgatccaga tggaccaatc aaggctataa
    18241 tttccttagg ttggaaagat agggaaacac tattcaaagc cttcttttta ttataataaa
    18301 cggacaggtc tgatacctgt aaaatcgcat ctgtcatacg gtttcctttc taaccaaagt
    18361 gaccagatac atagtcattg gtggactgta gcttggcatt ttggaaaata gttgcagtct
    18421 tgtcatactc aatcaaatca cccaagtaaa agaagcctgt atagtcactt gcacgagcag
    18481 cctgctgcat attatgcgtt acaatgatga tggtaaagtt tttcttgagc tcaaacatgg
    18541 tctcttctag ttgcatggtc gcaatcggat ccaaggctga ggctggctca tccattaaga
    18601 ggatatctgg cttaacagag atggcacgag cgatacagag acgttgttgc tgaccacctg
    18661 ataaggtcaa ggctgacttg tggagatcgt ctttaacctg atcccagagg gcagcctgac
    18721 gaagggaggt ttctacgatt tcatctagga cttgcttatc cttaactcca gcacgttcat
    18781 gcgcaaaggt aatattacgg taaattgact tagcaaatgg attggggcgt tgaaaaacca
    18841 ttccaatgtg tttacgcatt tcataaacgt tgatttctgg acggttgaca tcaattccac
    18901 gatagagaat ctgcccagtt actttagcaa tatcaatggt atcattcatg cgattgagac
    18961 tgcgtaagta ggtagatttc cccgatcccg acggaccaat caaagctgta attttatttc
    19021 tttcaaattg catatcaatc cccttaatgg attcattttt accatagtaa acatggacat
    19081 ccttagtaga aagggctact ttttcttcag gaaaggtaag gatatgcttc tcatcccagt
    19141 tatatgttga catggcttct cctttaggca gcggttaatt tcttgtgtag atagcttccg
    19201 aacttacgag ctccaaagtt aaaaatcagg ataaagatca ggagcacagc ggcagaacct
    19261 gctgatacaa tggttccatc tggaatagtg ccttcactat tgactttcca gatatggaca
    19321 gccaaggttt ctgcttgacg gaagatagag atggggctag tcacactgag gatattccag
    19381 ttagaccagt caagagctgg cgccgattgc cctgctgtat agatcagagc tgcagcttcg
    19441 ccaaagatac gaccagatgc caagacgaca cccgttacaa tacctggaag cgcttctgga
    19501 ataacaacat gaaccactgt ctcccagcga gaaatcccaa gagccagacc agcctcacgt
    19561 tgggtatggt gaacgtgttt caaactatcc tctacattac gcgtcatctg aggcaagtta
    19621 aagactgtca aggccaaggc acctgaaatg attgaaaatc catactcaaa ctgaactaca
    19681 aagatcaagt aaccaaagag acccaccacc actgatggta aagaggacaa gatttcaata
    19741 caagtccgca caaagttggt aacaggacct tttttagcat attcagccaa gtaaatccca
    19801 gctcccatag aaagaggtac agaaataatc aaggtaatga ccaataggaa aaaggaattg
    19861 taaagctgaa tgccaatccc accacctgct tgaaaagcag aagaccttcc agtcaagaaa
    19921 gaccaagaga tatggggcaa gccccgaacc aagatataga gaatcaagga agccaagatt
    19981 gtcacaatga tgctagcaat cgtatagagg acagctgttg caagtttatc taatttctta
    20041 gcgcgcataa tttttctttc ctctttcttt cgtaatcaat ttaatcacac tgttaaaaac
    20101 taaactcatc aagagcagta ccaaggccag tgaccagaga acattattat ttacagttcc
    20161 catgacagtg ttcccaattc ccatagttaa tatagaagtt aaagttgcag ctggtgtggt
    20221 caaggaagtt gggataacag ctgagtttcc gacaaccatc tggatagcta aagcctcacc
    20281 aaaggcacgc gccatcccaa agaccactgc agtgaaaata ccagaacggg ccgccttcaa
    20341 gatcacacgc cagatagtct gccagcgagt ggctcccata gcgaaactgg cttcacgata
    20401 ataacgagga accgcacgca agctatccgt tgtcataaag gttacggtcg gcaaaatcat
    20461 gacaaagagg acggaaatcc ctgacaaaat cccaaaacca gtcccaccaa agacactgcg
    20521 aacaaaggga acgacgactt gcaagccaat aaatccgtac actactgaag gaatcccaac
    20581 caggagttca atagctggtt gcaaaatctt cgcccctttt ggtgatactt cggtcataaa
    20641 aactgctgca ccaatagcaa agggggttgc gataagggct gagagaatgg taacgataaa
    20701 ggaacccaaa atcataggaa gggcaccaaa ttctttacta gaaggattcc aaattcctcc
    20761 caaaagaaag tcaaagatat tcacaccatt gacaaagaag gtcgacaagc ctttttgcgc
    20821 tacgaaaacc aaaatcatgg ccacaaggat gactatcaaa gaaagacagg caaaggtcaa
    20881 accttttcct aatttctcca gacgagaatt ctttgatgga agcaacattt tcttagctaa
    20941 ttcttcttga ttcattattg tctcccttcc aacactgtca cagttccggc agcatctttt
    21001 tcaaccttca tttccttaat cggaatatac ttcaatcctt tgacaatccc ttcttgggtc
    21061 tcatccgaga gaacaaaatt gagaaattct gcagccaact cattgggctg ccccaatgta
    21121 tacatatgct cataagacca caagggccaa ttattgctac ttatattttc tggacttaag
    21181 tcatagccat tcaacttcat gcttttgacc gaatcatcta tataggtaag agataaataa
    21241 gagatagctc ctggactttt tgatacgatt gattttaccg ctccatttga atcctgctcc
    21301 tgactttgca tggcagactg accttccata atgacagtat caaaggtagc acgagaacca
    21361 gagccggctg cccgattgat aacagagatg ggtaagtcct taccaccaac ctctttccaa
    21421 ttggttacct cacctatgaa gatttgacga agttgctctg tcgttaggtt atcaacatca
    21481 acctccttat tgacaatcag agccaagcca gctaccgcga ccttgtggtc aacaagagca
    21541 gaagcatcaa ttccgtcttt ttcctcagca aatacatctg agtttcctat atcaactgcc
    21601 ccagactgaa cctgggacaa gcctgtacca gaacctcccc cttggacatt gaccgttttt
    21661 ccaacatgga tcgtgccaaa ttcatctgcc gctacttcaa ccaagggttg caaggcagtt
    21721 gagccaacag ccgttatgga ttctccacga tcaatccagc tagcacagcc tactaaacaa
    21781 gccgtcagcc aaaaagcgat aagagacaga gcaagctttt ttcttttttt cactgttttt
    21841 ctcctcgaaa ataattatga atactgtgaa ttttttaagt agttctttat gagttgacgc
    21901 atgaattctt accaaatttc tgcgcaattg attatttata taatataggc tatattactc
    21961 tttcctaacc tccttttttc atatgtggat aaaatctctt gtctatccct tcccccattg
    22021 tcacccatta tagtcatttc gtgtctcttt ttcccctttt taatgcaagg gaaattactc
    22081 tccttagatg ataatccaaa agctagaaag gtatctcaaa cctctctact ctcccagact
    22141 agtttacaac taaaaggaaa agattctatt ttatgagaaa tctagtttac aagcggtaag
    22201 aacgctaata actaaacttc ttgtactctt tgaaaatctc ttcaaaccag tgttttgagc
    22261 tatctatggc tagcttccta gtttgctctt tgattttcat tgagtagtaa aactacatgt
    22321 aatggcaatc aagatatcaa gaatcatcct actaaaaaaa tccatacttt cactataaca
    22381 tagaataaga tatttgacta gcattttcat ttgaatctga ggccttttgg aaaataattt
    22441 ttcaaaacat ttccagtaac ctttgcaaag cccaagccat tgcctttaac caaaacttgg
    22501 taccaaccat ttggcagact ttctgccagc tgaacgattt ctccagccgc atacttgaca
    22561 aacgcttctt ggccaatttc aaccgactgt tcgacctgac tcgatttcaa ggctaaacca
    22621 agagcgaaac tgggctcaaa gcgtttcttc ttaaaagtac ccagatgcag tccattgcga
    22681 gcaatcttga gcttccctaa atctggcaaa agttctggca agagataaag ctggtctcca
    22741 aaagtctgca agatacccgg tagattgacc ttcaaatggt tttgggcaaa ttcctgccac
    22801 aaggcaactt gttcacggct gaggttactc ttacttgcct taaatttagg agctggattg
    22861 ttacccttaa actgtagatg ggcaacaaac tgaccctctc ccttaaactg atgagggtac
    22921 atccgagccg tttctggcag gtcaatacca gctaccattc cattgatatg ctctactggc
    22981 aacaagtcaa aatcatactc ttccagcaac caattgacaa tctcttcgtt ttcctcgggt
    23041 gcccaggtac aggtcgaata aaccagacga ccaccttcag ctaacatggt cactgcatcc
    23101 tccagaattt ctctttgcaa gctagcacat tgactcggat aatctaagct ccaatagtcc
    23161 atagcatcag gttgcttacg aaacattcct tcaccagagc aaggggcatc aagaacgatt
    23221 aagtcaaaat agcctttaaa gaccttgacc aagcggtcgg cagattcatt ggtcaccacg
    23281 acatttgtcg ctccaaaacg ctccatgttt tcaaccaaaa tcttagcccg tttgcttgaa
    23341 atttcattgg aaacaagtag cccctcccct gctagatagg ctgccagttg agttgatttg
    23401 ccccccggtg cagcagccaa gtccaagacc ttcataccag gactgggttg ggctacttga
    23461 gccaccattt gagcagcagg ttcttgcgaa taaactaaac ctgtagcatg ctcaggcgat
    23521 ttccctgaaa ccttcccata gtggccccaa ggggtttgag taatggcatc agaaaaggaa
    23581 agttgctctt cttttaaggg attgacccga aaggccgaaa ccgcttcctc ctcaaaagag
    23641 gcaagaaaat ttcttgcctc atctcctagt atttctttat atttttcaac aaatccttct
    23701 ggaaattgca tttaagttct tttcctttcg taaatatagg actgaatttc ctcctgcatc
    23761 tcaagaggca ccatcatgac cggctgtctg gtttgaaaat caggagcttc accaaaaagg
    23821 gtcacaaccc gatagcccag actttcccct aaaatactag ctgcggcata atcccatggt
    23881 tgcagataag tgagataggt caacaaacgc cctgacaaaa tcttggcaaa actaatggcc
    23941 gcacttccat agacacgaac accaagaacc gctcggctca aatcagccag cccccattca
    24001 ttggtttcca gcataccact attccctgca atgagaaaat ctccaagtgg tttagtttta
    24061 aaaggagcta ggggcctatc atttagacaa actggaaatt ccccaccacc gtggtaacaa
    24121 tcccctttga ccacatcata aatcagacca aactgtccct gaccattttc aaaataagcc
    24181 atcataacag caaaatcttc ctgctgggct acaaaattat tggtaccatc aatgggatca
    24241 atgacccaaa ccttgccctc ttgaaccgag gctcgcagac aaccttcttc agcacaaatc
    24301 ttatcctcaa gataacggga caaaatctca ccaaccaaga gttcctgaac ttctttgtcc
    24361 agtctagtca ccaaatctgt tggagaggac ttggtttcaa cacgcaagtc ttcctgcata
    24421 tggtcaagaa tgtactgacc tgctttctta acaagctctt tagcaaattc aaatttactt
    24481 tccaagagaa atctttcctt cccctttttc tttggcagcc tgaactgctc ggtaaagtga
    24541 atagccacta accgtttcaa attctcgtcc caaacgtttc tcttcgctct tgcttggcac
    24601 gaccgctttg aatcccttat aggagtccag taacttttta gcctctactt tgccttcata
    24661 ggcagcttca acatcattaa aaaaagaaag cactgaagca agttcttcag tgctccacga
    24721 caaatctagt gggtaactat actgtttgtt cattaactaa taccagctct cattcttgct
    24781 tcttttagtt cttgcttacg ataactacga gggagaaaag cacgaatctc atcttcatta
    24841 aaaccgattt gcatacgctt ggcatcaata ataattggac gacgcaaaag actaggatac
    24901 tgctcaatca aatgaagcaa ttccgatacc gaaatactct ctacatcaat attcaatttt
    24961 tgaaaaattt ttgaacgagt tgaaatgatg tcatcagtac cattttcggt caaggaaagg
    25021 atgtgttgca attcttttct tgttaaagga ctggtcataa tattgtgttc cacaaaggga
    25081 accttatgtt tttctaacca ggccttagcc ttacgacatg atgtacagct cggtgataga
    25141 aatagtgtaa tcatgctttt ctcttcttat ctatactttg ctacttctat tatacaaaaa
    25201 aataaagcgc ttgactaggg atttttagaa aaaaagccta ttttttcaag aaaaataggc
    25261 tttttgcgaa cgattgacac aattggattt ggttaattca ctcttaacga tggttttaaa
    25321 tgatatatat ttttatatat gtaaattaaa aacatctttc ctttcacttc ctacgacttt
    25381 tcagatacag atagccaaag aagttttcat agagggcaaa aaagaggagg aaggcatgaa
    25441 gaaagaaggt ctctggcaaa atcataataa caggatcctt ggctggatca aaaagccagg
    25501 tatcatctcc cacaaagaga atttgatgga aaagagtaaa gaattggtca aaaccaatca
    25561 aaactccccc aagtccaatc atcacaggta agactactag agccaggaga ctttttcgat
    25621 aaagagacaa aaagtccttt ttcacaatcc tattgacaaa gacatagaaa cttggcagtg
    25681 tcactagagc tactagctga accaaatgaa agagattctt gaccactgcg aaatggtgca
    25741 gaccagctgc tgacgaacga aaatcaggca tctgtaagac ctgactaaaa ggattggtca
    25801 gataattcat caagatatga aaattgtatt gaatggtttc tggttttaga tagactcgat
    25861 tcgttaagtt tagccactga atctccatag gatagaaaat ccaagccaga taaatggtca
    25921 gaaggattga gagggagagg agaaagagca tagagcccca aaaggtcaat ttagttttca
    25981 tcaaaatccc actccgcaag gctagaaacc acatgtgtcg gtgcgattgg caggccagct
    26041 acttcttctg ccttagtaaa acctgtcgtc accaagagcg ttggaatgcc attgtcaatc
    26101 ccagcccgga tatcagtcaa ataattgtcc ccaaccatga ttaactcttc acgttccaaa
    26161 cctaagtgct caaccgcctt gtccataatg atggcatttg gttttccgat ataaaccggc
    26221 ttcactcgtg tcgctacttc aagcagcgta atcagtgagc cagcacctgg caaaagaccg
    26281 cgttccgtcg ggatgttgag gtcaggattg gttccgataa aatgggcacc cttttgaata
    26341 gcaagagttg ctgtggcaaa tttttcatag tcgacttgcc aatccagacc aactaccacg
    26401 taggcagggt tttccttgtc ttccacataa ccagccgcct tgatggcttc cttgagtcct
    26461 gcttctccga cgacatagac ggtcttttca agccccaaat cattcatata gtcgatggtt
    26521 gccaaagtcg ctgtgtagac agtcgatagg ggcgtatcga tattaaaatt ctgagccaac
    26581 atctccttaa cactctctgg agtgcgagtt gtattgttgg ttacaaagag atagggaatg
    26641 ccccgctttt gcaattcatg aacaaaagtc tctccagcag ggattcggtc tttcccctta
    26701 taaatggttc cgtctaaatc aattaaatag cctttatatt tcatctattt ctccctaagc
    26761 cttttttatt tcttgccaag taatgattgc ttgggcattg ataaccccat cacttgtaat
    26821 ttcatgcttg ctttccagtc cagtccgttc aacaaccgat gtaatcactc cacctggtcg
    26881 aacttccttg acatacttga ggttgatttt cttgggaata tagtgggtca aaaaatccgc
    26941 tcccatgacc tcaaaaatcc agtccaagta tttactgtta ttgacatgac cattcatatc
    27001 caagtcgtaa aaacgaacat ggtaatcctt gctgatcggt tcttccaagg actcatactt
    27061 cggtccacgg ataagttttt tatcaaaatc agactggtaa ggagccacaa tctcaggttc
    27121 aacagcatgg acttttcgac tgtcgcggtc catgagaaca aaggtcgcca tcatatggat
    27181 gagctcctgc cccgcttcat tataaatagt aaagcgacgg tagcaaaaaa gtcgattgta
    27241 gctcaaggct tccgtttcga tggtaatttc ttccgcaaaa cgaggcaaac gaaccacctc
    27301 aatatcatat tctgcgataa tccagaccag attatagtct tccaaaatgg ccttatcact
    27361 aactcccagt tcaatcgact gcatccctga aacttgcagt gacagcaaaa tcacatctgg
    27421 aagtttgata tgaccgttca tatcagccat atcaaaagga attttcattt tcatttgata
    27481 agttaagccc atgatcctac tccaaaataa atcgttctgc tacagtatct cccaaaaaga
    27541 gacctctctt tgtcatgcga acgtggtcac cctcaatctg catgaggcct tgttgaacca
    27601 aatctctgac aatttctcca taaagtccag caaaagactg tccaaatttt tcctcaaatc
    27661 gcgccatgga aaccccggat ttcttgcgga gtcccaagaa catttcttct tccatttgct
    27721 ccttttgact caggtgatct tctgtaatac aagcattgcc ttcctcaacc gcactgagat
    27781 aatgacgaat gggaccatga tttttataac gtactccatt gacataacca gatgcccctg
    27841 caccaatacc atagtattca gcattgtccc agtacatgag attatgacga ctttcaaagc
    27901 cgggtttgga gaaattagaa atctcataat gctcaaaacc cgctcgctcc agctctgcaa
    27961 tgatgtactc aaacatctcc gcttctagtt cctccttagg cagaggcaat ttcccacgtc
    28021 gcatccggtt cataaagacc gtatggtttt ctaaaatcaa actatacaaa ctcatgtggg
    28081 gaatatccag tccaatggct ttagccacat tttcctttac ttgctccatg gtctgaccag
    28141 gcagagcata aatcaaatca atggagatgt tgtcaaaacc agccagtttc agacgatcga
    28201 tattttcata aatatccttc tccaaatgac tgcgcccaat ctttttcaac atcttatcat
    28261 caaaggtctg gacacctagc gaaacacgat tgacagccga atttttcaaa acagctatct
    28321 tatccgcatc caaatcgcct ggattggctt caatggtcaa ctcttccaag acagacaagt
    28381 ccaagttttt agtcaagcca ttcagtaaca cctccagttg cggagccgac agggctgtcg
    28441 gtgttccacc accgatataa agggttgaca acttttcaat atcataagaa cgaaactctt
    28501 ccagcagatg ctctaaatag ctgtcgactg gctgattttt gatgaagacc tttgaaaaat
    28561 cacaataata acaaatctgg gtacaaaatg ggatgtgcac ataggctgac gttggttttt
    28621 tctgcatagt aattattata ccacaaagac tagattccag ataaaaatca ccatccccag
    28681 atacatagtc cgtccggaga tggtgatggt ttattcttct gttatatcaa tcacaatctc
    28741 ttctgagtca tcaagagctt cggctttttc ttgccattgt tccttgagat tatttaattg
    28801 attttttgat gcttctgtcg cttgaaaagc ataggattta gcttgagcaa gtatactgtc
    28861 cacagtgatt tcacctgact caacctgttc ttttgttttc agaacaaaat ctgtagcctg
    28921 ctccttaact tctgtcagtt tttcacagac ttgctccttg gcatactccg gatcttctct
    28981 caaatcatct agaaaatctt gagcctgact gcaaacttgt ttgcccttat cacttgttaa
    29041 aaacaaggca agagctgcac ctgaaaccgt tcctaaaagg attgaggata atttacccat
    29101 aaggattctc cttttttatt ttttgaaaaa tttacttgca agacgaagag ctgacagact
    29161 tgcaccagtc ttgagtgttt ttgaaccagc tgatgaagct ttcttgctca agacacgcgc
    29221 atggtcattg aggtctgaaa cagatagaga taaatctgca acagcactga agagtggatc
    29281 aattgtagcc accttgacat tgatatcatc tgccaagaca ttgaccttag ccaacaactc
    29341 attggtgtga tgcaaggtca catccacatc tgaagtcaag gttttaatcg tcttttctgt
    29401 ttcatcgatg acacgaccaa gcttttgtac agtaatgatc agatagacca aaaagacaat
    29461 caaagctagg gcaacaagaa tatatgcaac ttctaacatt tagttttcct cctctgtaat
    29521 atagtaaggg gccttctttc gattttgata aataacgatc attataccga gaccgataag
    29581 gacaactgac agccattggg acactcgaag gccgaagaac atgagactat ctgttcgcat
    29641 accttcgata accatacgac cgaaaccata ccaaatcaag taaaaggccg tgatatgacc
    29701 tcgtctgaga ctcttccatt tccgtctaaa aatcagaatc aaggcaaagc caagcagatt
    29761 ccatagagac tcataaagga aagtcggttg acggtagctc ccctcaatat acatctggtc
    29821 acggataaag ccaggtagat aatccagatt atccactgtt gcaccataag cttcttggtt
    29881 aaagaaatta ccccaacgcc ccaaactttg agcaatcata acgctaggcg ccgcaatatc
    29941 tagaaaatcc caagtattga tgagtttacg gtcagcaaag atatagagca caagaacccc
    30001 agttatcaaa ccaccgtaaa tggccaaacc accattccaa atggcaaaaa tctctcctaa
    30061 attctgacta tagtaatcaa atcggaaaat aacatagtag agacgagctc ctaaaatagc
    30121 caagggaaag gctactaaga taaaatctaa aatatcgtct ggtatgatct tctttctagg
    30181 tgcttctttc atagtcaaat aaaccgcaag aatcaagcct gtcacaatac ataaggcata
    30241 ccaacgaatg gctaggggtc ctagttgaat agcaattgga tcaagcattt tgcacctcat
    30301 ttcgagcgat tagacttgtc agtcgttcgt cgaacaaacg ggtcgcatca aagcccattt
    30361 ccttggcacg ataattcatg gcagctgcct caatcacaac agagatatta cgacctgttt
    30421 taactggaat acgaatacga ggaatggcta cgccagaaac ttcaagttcc tctgcattgt
    30481 ttccaagacg atcaaaggtc ttatgcgtat cgtaattttc caaatagaca gcaagctgaa
    30541 cctgtgaaga atccttgaca gcactcgcac cgtagagact cataacatcg ataataccaa
    30601 ccccacgaat ttcaatcaag tgtttcaaaa tttcagctgg ttcaccccag agagtaatct
    30661 catccttggc aaagatatcg acacggtcat cggctaccaa acggtgacca cgtttgacaa
    30721 gctcaagacc tgtctcgctc ttaccaattc cactatctcc ctgaatcaag acgcccatcc
    30781 cataaatatc catcaagaca ccgtgcacac tggtacgttc tgccaaacga gaatccagat
    30841 agctagataa ctctccagat aaacgactgg tagctgcacg gctggttaaa atagcaatct
    30901 tacattctct agcagccttt aacatctcct ctggaaccac caaaccacgg gcaacaatga
    30961 ctgctggtgt ctcaggtaga aacatttttt tcaaaacttc ataacggctg ttggaaggca
    31021 tgctgatcag ataagaccac tccttcatcc ccaaaagttg aatccgctct ggtgtatagt
    31081 agtcaaaata gcctgtcatt tcaagaccag gtcgcgtaat atccgctgta ttgatttcct
    31141 tttcaagcaa ctctggttca ccatagacaa tatctagtct aagcttttca atcacttctt
    31201 ttactaaaac cgacatcatt tcctccttca aatgagttct tctctctatt atatcaaatt
    31261 gtaggaggaa gtttcagatt tttgcaggct ttggagtatt tatttaaaag aaaaaggact
    31321 agatttctct agcctttcat tgcaattaga attttttacg tttacgagct acttctgatt
    31381 tacgtttacg ttttacagaa ggtttttcat agaattcacg tttgcgtgtt tcttgaagag
    31441 taccagcttt agtaaccgca cgtttgaaac ggcgaagtgc gtcgtcaaga gattcattct
    31501 tacgtactac tgttttagac atttttttca ctcccttcaa gttcaatatc tatttcattc
    31561 tacacgtaaa aggaataatt gtcaagcaaa aatgcggttt aattttgatt ttttcttcca
    31621 tttatacaag agttgaaagc gttcaataga acattgcttt tttatttttc gagtaagcta
    31681 agcgcttcag catctgcgat gatggttaca tcagggtgat tttggaggct acttgcaggt
    31741 aggttctcag tcactgggcc agatactgtt ccggcaatgg cttctgcttt cgactcaccg
    31801 taagcaaaaa gaataataga cttggcatcc aaaatgtttt taatccccat tgaaatagct
    31861 tgggttggga cgtcttcaat cttggcaaag aagcgtgcat tggcttcaat agtagactgg
    31921 tcaagttcta ctagatgcgt ttgactgtca aatggagtac caggctcatt aaagccgata
    31981 tgcccattgc gaccgattcc caagatttgg aggtcaactg gatggtcagc caaaatttgg
    32041 ttgtagcgtt caacttcagc ttcagcattg tccttaacac cacgaggcaa gaaactttct
    32101 ttaaatggtt tttggttgaa caagttttct tgcatgaagt aacgataaga ctgtggattg
    32161 tccccatcaa ggcccacata ctcatcaagg ttgacactgg ttagatttga aaaatcaagg
    32221 ttactctcaa caatttcctt gtaaaattca agtgggctac ttcctgtcgc aagtcctaga
    32281 gtttgagctc cattagccaa tttttccttc aaaatctcaa aagcaacttt tccaccttga
    32341 acttggtttt caactttaat aactttcatc ttttcatcct ccatttctat ctatatttat
    32401 tatatggtat agaccaattt ttgtcaagtg aaaacgattt aatttctaaa aaaagagcgt
    32461 ccaaacttga aacgtgttat aatggatagg attagaactt agaaagaatg aacagatata
    32521 tgaatacagc tgattttgat ttccacttgc cagaggaatt gattgcccaa acgccccttg
    32581 aaaaacgtga tgcctccaaa ctcctcatcg tcaaccgtga gacaggagaa atgcaagata
    32641 aacacttcca ctctattatt gatatgctgg aacctggtga tgcccttgtc atgaacgaca
    32701 cccgagttct ccctgcccgc ctctatggtc aaaaagtgga gacaggaggt catgtggaac
    32761 ttctcctcct taagaacact agtggagacg agtgggaagt tctggctaaa cctgccaaac
    32821 gcctcaaggt cggtactcgt atcagctttg gtgatggccg cctcagcgct gtcgttacag
    32881 aagaattgac ccacggggga cgcattgtcc gctttgaata ccaaggaatt ttcctagaag
    32941 tcttggaaag tctgggagaa atgcctctgc caccttatat ccacgaaaaa ttagatgacc
    33001 gtgaacgtta tcaaaccgtc tacgccaagg aaagcggctc tgctgcagca ccgactgctg
    33061 gtcttcactt caccaaagaa ctgctggcag aaatccaagc taagggtgtt catctagtct
    33121 atctgactct ccatgtcgga ctcggaacct ttagacctgt ttctgtggat aatctggacg
    33181 aacacgaaat gcattcagag ttctatcaac tttctgagga agctgctgcc acccttcgct
    33241 ctgtcaaaga aaatggtggt cgcgtcatcg ctgtcggaac cacttctatc cgcaccttgg
    33301 aaactattgg ttccaagttt gatgggcaaa tccaagcaga ttctggttgg accaatatct
    33361 ttatcaaacc tgggtatgag tggaaggtcg tggatgcctt ctcaaccaac ttccatctgc
    33421 caaaatcaac tctggtcatg ttggtttctg cctttgcagg ccgtgaatta gtcttagatg
    33481 cctaccacca tgcgatccaa gaacactacc gcttcttcag ttttggtgac tccatgttta
    33541 tttattgaga aagaatttct ctaaatcttc taataccaat aaatcgctaa gatattattt
    33601 caaagaacat ctacaattga aactctagct agctgtagaa gaggcctagt acattgaaat
    33661 taaaatgctt ccccctagct tcgaaaatat tgccatagat tgcgttgact ctccaaattg
    33721 attcatctat attttatttc agcttcctat attttcttcg ctgtttgtaa atcaaaatgc
    33781 aagacacatg agtagcacca tatttgttac tcttatctgt cctctcaaga gactattatg
    33841 agttatttca gaatcattca ctactttgac cctgactctc cttagtctca aaatcaaaga
    33901 cttatactct tcaaaaatct cttcaaaccg cgtcaacgtc accttggatt atatatgtga
    33961 ctgacttcgt cagttctatc tacaacctca aagcagtact ttgagcaacc tgcgactagt
    34021 tttctagttt gctctttgat tttcattgag tattaaacaa aaagtgaaca aagctgaatt
    34081 ctaatgtaca gaagactagg cttgttcact tttttatagt cgctataaga tgaccttatc
    34141 tatagctttt tatatataat tatatattca gacatactat tatcaatttt gtcgcaggga
    34201 ggaatctgtt aacgcaccca ttcaccatta tcattgactc tatagccatc tatacttgta
    34261 ttgaccgcta actcacccga tgcatttaca taataccatt taccaccaac ttggaaccat
    34321 tgattgactt tcatagaacc attgctgttg aggtagtacc atgaaccatt aacttgtacc
    34381 caacctgttg ccatggagcc atcagtatta taaaaatacc acataccatt ttcttgtttc
    34441 cagcctgttg ttggagcaac tgctttagct ggttctactg ctacatctgt tccttggtta
    34501 gatgtaacag atacaggata tgaaggaata gatgattgct caggaacaac aactttttca
    34561 ggttctctcg tccctctcct tatacgtctt tttaccatct ctttagtaat ttgacgagaa
    34621 gtagtttctt caattgttcc atcacgttca tctacagcat agattgtagt aagagtaatt
    34681 ttaccaattt ctcctacttc ttgaccttta tcaagagttg ggtcatcgag atattctgtt
    34741 tcgattggaa tttcttggac aagaacttgg ggcttggttc cttttttaac aactcttgtt
    34801 tgagagtctt ctttttgact taaagtactc tcagttactt gtccactctt tccatctaca
    34861 tcataagtta tcgttgtaac tgttttccca ttctttccta gagtaatctc ttgctcctgt
    34921 cctgcagaaa ggtcattgtc tgcttcatat tcagtagtaa atggaacaag aacttcttca
    34981 accttgcttt tagctggaac tttgataact gtatccgtgg cttcttttct atcaacagta
    35041 acctgttcgg taacataacc agtctctgga ttaacatcgt aggtccttgt cgtagttaca
    35101 tagccatcct ctccatcaat tgtaacagga ttttcactac ggtcttttgt ttcatctttt
    35161 tcataacgaa ttcgcgtact tgaaattttc ttggttacta ccttaggttt agtcgctact
    35221 tttacaataa tatccccatt gtcagcgtca tcatactcta ttccctcttc tttatctcta
    35281 gtatcatctc tgacatattg aatcccatca gcagcatgaa caaaacttgt attcagattc
    35341 ctcctaaaaa taaagttagc ccgattaccg cagaaccaaa aatctttccg agtttacgta
    35401 ttgcatagcg cttattagta ttagattttg ccattacatc ctacttctag tatag
//
LOCUS       DAYYQN010000013        34899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYYQN010000013 DAYYQN010000000
VERSION     DAYYQN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.013"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            246..542
                     /locus_tag="ACHBRQ_000598"
     CDS             246..542
                     /locus_tag="ACHBRQ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HFM9592313.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            619..2649
                     /locus_tag="ACHBRQ_000599"
     CDS             619..2649
                     /locus_tag="ACHBRQ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HFM9592314.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQGLFTDYL
                     QNFSSESVEVTSQKYYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDCLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            2651..3292
                     /locus_tag="ACHBRQ_000600"
     CDS             2651..3292
                     /locus_tag="ACHBRQ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592315.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            3285..3578
                     /locus_tag="ACHBRQ_000601"
     CDS             3285..3578
                     /locus_tag="ACHBRQ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592316.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            3829..4701
                     /gene="rsmA"
                     /locus_tag="ACHBRQ_000602"
     CDS             3829..4701
                     /gene="rsmA"
                     /locus_tag="ACHBRQ_000602"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9592317.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            4703..5581
                     /gene="rsgA"
                     /locus_tag="ACHBRQ_000603"
     CDS             4703..5581
                     /gene="rsgA"
                     /locus_tag="ACHBRQ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9592318.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            5592..6248
                     /gene="rpe"
                     /locus_tag="ACHBRQ_000604"
     CDS             5592..6248
                     /gene="rpe"
                     /locus_tag="ACHBRQ_000604"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9592319.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            6211..6873
                     /locus_tag="ACHBRQ_000605"
     CDS             6211..6873
                     /locus_tag="ACHBRQ_000605"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9592320.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            6875..8131
                     /gene="rmuC"
                     /locus_tag="ACHBRQ_000606"
     CDS             6875..8131
                     /gene="rmuC"
                     /locus_tag="ACHBRQ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFM9592321.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            8121..9062
                     /locus_tag="ACHBRQ_000607"
     CDS             8121..9062
                     /locus_tag="ACHBRQ_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HFM9592322.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            9329..10156
                     /gene="purR"
                     /locus_tag="ACHBRQ_000608"
     CDS             9329..10156
                     /gene="purR"
                     /locus_tag="ACHBRQ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9592323.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            10223..11473
                     /locus_tag="ACHBRQ_000609"
     CDS             10223..11473
                     /locus_tag="ACHBRQ_000609"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9592324.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            11599..12393
                     /gene="pflA"
                     /locus_tag="ACHBRQ_000610"
     CDS             11599..12393
                     /gene="pflA"
                     /locus_tag="ACHBRQ_000610"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9592325.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(12468..13394)
                     /gene="yidC"
                     /locus_tag="ACHBRQ_000611"
     CDS             complement(12468..13394)
                     /gene="yidC"
                     /locus_tag="ACHBRQ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9592326.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(13472..13750)
                     /locus_tag="ACHBRQ_000612"
     CDS             complement(13472..13750)
                     /locus_tag="ACHBRQ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9592327.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            13784..14524
                     /locus_tag="ACHBRQ_000613"
     CDS             13784..14524
                     /locus_tag="ACHBRQ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9592328.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            14580..15263
                     /locus_tag="ACHBRQ_000614"
     CDS             14580..15263
                     /locus_tag="ACHBRQ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFM9592329.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            15375..16367
                     /gene="asnA"
                     /locus_tag="ACHBRQ_000615"
     CDS             15375..16367
                     /gene="asnA"
                     /locus_tag="ACHBRQ_000615"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFM9592330.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            16432..16971
                     /gene="rsmD"
                     /locus_tag="ACHBRQ_000616"
     CDS             16432..16971
                     /gene="rsmD"
                     /locus_tag="ACHBRQ_000616"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9592331.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            16961..17449
                     /gene="coaD"
                     /locus_tag="ACHBRQ_000617"
     CDS             16961..17449
                     /gene="coaD"
                     /locus_tag="ACHBRQ_000617"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9592332.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            17433..18470
                     /locus_tag="ACHBRQ_000618"
     CDS             17433..18470
                     /locus_tag="ACHBRQ_000618"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9592333.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            18528..18758
                     /locus_tag="ACHBRQ_000619"
     CDS             18528..18758
                     /locus_tag="ACHBRQ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9592334.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            18782..20065
                     /gene="murA"
                     /locus_tag="ACHBRQ_000620"
     CDS             18782..20065
                     /gene="murA"
                     /locus_tag="ACHBRQ_000620"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9592335.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            20058..20246
                     /locus_tag="ACHBRQ_000621"
     CDS             20058..20246
                     /locus_tag="ACHBRQ_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9592336.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            20285..21109
                     /gene="endA"
                     /locus_tag="ACHBRQ_000622"
     CDS             20285..21109
                     /gene="endA"
                     /locus_tag="ACHBRQ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HFM9592337.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            21400..22731
                     /locus_tag="ACHBRQ_000623"
     CDS             21400..22731
                     /locus_tag="ACHBRQ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9592338.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(22929..22999)
                     /locus_tag="ACHBRQ_000624"
     tRNA            complement(22929..22999)
                     /locus_tag="ACHBRQ_000624"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22965..22967),aa:Cys,seq:gca)
     gene            23415..27026
                     /gene="rpoB"
                     /locus_tag="ACHBRQ_000625"
     CDS             23415..27026
                     /gene="rpoB"
                     /locus_tag="ACHBRQ_000625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9592339.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            27059..30736
                     /gene="rpoC"
                     /locus_tag="ACHBRQ_000626"
     CDS             27059..30736
                     /gene="rpoC"
                     /locus_tag="ACHBRQ_000626"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9592340.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            30847..31260
                     /gene="ndk"
                     /locus_tag="ACHBRQ_000627"
     CDS             30847..31260
                     /gene="ndk"
                     /locus_tag="ACHBRQ_000627"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9592341.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(32189..32821)
                     /locus_tag="ACHBRQ_000628"
     CDS             complement(32189..32821)
                     /locus_tag="ACHBRQ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592342.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(32823..34838)
                     /locus_tag="ACHBRQ_000629"
     CDS             complement(32823..34838)
                     /locus_tag="ACHBRQ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM9592343.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT        10172 a         6836 c         7986 g         9905 t
ORIGIN      
        1 tttttagata taagctaaaa ataatatgaa atagttagat tttaaggaca ttgatgtcct
       61 tgatagtgtg ataaagatgg agtataatga ctataaaagt tcatttcagg gtgctatttt
      121 gacaatttag agaaaaacaa ataattttag aatatcattt ggtaatataa tagtatcaac
      181 gttgataatg gaaagaggag caagtaactc aacaatcttg taatcaaaaa tagaggagta
      241 tcaatatgaa acaaacagtt aaaaagttag ctcttgtagc gagcattgca gcaacattag
      301 gtggaggtgt atcagttgcc tctgcagccg ttcaataccc agaaggtggt gtttggactt
      361 atggttcagg taacggtggt gcttactcaa actactatca cccttcaaaa taccatagtt
      421 caacagttgt tagcagaaaa actggttcat ctgacaaggg atatgctggt gctggaggga
      481 cttctcgtgc atggattcgt acttcttggg gagagaaagt tgcattctac tataatgttt
      541 agaatggtat agccctattg tttagcttta cttggtcacc ttctcactaa gtgaccaagt
      601 aactttttgg aggaaatgat gaaacgttta tttattttga tttcaatggt attggtatcg
      661 ctttatatgg tgataacttc cgttgaccat cgagaagaga ttttatttgg taactatcct
      721 tctgttgatg tgacaggaat gatgataaat caaccagtag ctagtcgcga agaggtgaca
      781 gaggctttga gtcacttggc ggtagagcat aatagtctca tcgcccgtcg aatcgttgag
      841 ccaaatgaag ctggagaaac actctttacc tatgccactt atggtgaggg agagcttcca
      901 gaaggtctga ccatttcctc caaggagagt gcagaaacga gtgatttatt agggtcttac
      961 ttgattgtgt caggaagttt ggatggagtg agcttacaga ccaccttgaa agaacttggc
     1021 tatcaaggct ttgtttcgaa tggagaagat ccattttcga tagtcttact attgacggcc
     1081 acccctatgg tgctactgag tttagctatt tttctgctga cctttatgag tctgaccctg
     1141 atttatcgga tcaaatccct tcgtcaggca gggattcgct taatagctgg tgagagcttg
     1201 tttggagttg ctctcagacc agtgttagaa gatgtgagac agcttatctg ctcagtgctg
     1261 gtatccagtc ttttgggatt ggggattctc tggtatcaag gtgccttgtt tatggcaacg
     1321 gtgcaactgg tcatcattgc tcttctactt tatggattga ccctggcagg gatttctacc
     1381 ttactaagtg tcgtctatct acttggttta caggaaaata gtctggtgga tctattgaaa
     1441 gggaaactcc ctctcaaacg tatgatgaca ttgatgatgg tggggcaact cttagctgta
     1501 ttggtggtcg gatcgagtgc gacagctctc ctaccccact accgtgaaat gcaggaaatg
     1561 gagagagcta gcaataaatg gagccagtcc tcagaccgtt accgtctatc ctttggttgg
     1621 tctagtgcat ttgccgatga agaaggaacg cgtaaggata atcgtgagtg gcagacattt
     1681 actgaagaac ggttagccaa tacagactct ttttatatta tgagcaatgt tgacaatttc
     1741 tcagatggag cagaagtgga cctagatggc aatcgtctca gtgactacac accgtcaggg
     1801 aatgttatct atgtctcacc acgctatctg atagaagaaa agattaccgt ttcttcagag
     1861 tttatggaca agatgcaaaa cttgtctgag ggagagtttg ggctgatctt gcctgagagc
     1921 ttgcgagagc agtctaccta ctaccaagga ttgtttacag attacctgca aaacttttca
     1981 tctgaaagtg tagaagtgac gagtcagaaa tactacctcc cacaggtaag gctagctttt
     2041 acagaaacag gacaggaacg tttcctctat aatgatgggt acaagacaac acgccagtac
     2101 ctaaaagatc cgattattgt agttctaacg ccgcaagcga ctggaacaag acctgttgca
     2161 gggatgttgt ggggaactac ggctaatagt gccttgaaac tagatcgata tggagacagc
     2221 atcacagctc taaaagagca aggtctgtat cacaaggttt cttacttggt aaaaagccag
     2281 ctattttttg ccaaggtact aaatgacaaa cgggtggagt tttactctct ccttattggg
     2341 acgattttga ccctgtctac ggctatcttg ttatttgatt ccatgaatct tctctatttt
     2401 gagcagttca gacgggaact tatgattaaa cgtcttgctg gtatgacaat ctatgagctt
     2461 catggcaagt atttactggc gcaaggagga gttctcttgc ttggcctagt cctatctagt
     2521 attttgacaa gagattgttt gattagcgct ctagttgtag ctttgtttac gcttaacgcc
     2581 ctcttgattt tagtaaggca ggacaaaaaa gaagaagctg gtagcatggc agtattgaaa
     2641 ggaaaataag atgattgata ttcaaggatt ggaaaagaaa tttaatgacc gcgcgatttt
     2701 ctctggtttg aatctcaagc tggagaaggg caaggtttat gccttaatcg gaaagagtgg
     2761 aagcggaaag acgacgctgc tgaatatctt gggaaagcta gaaaagatag atggtggaag
     2821 ggttctctat caggggaaag atttaaaaac cattcccact cgtgagtatt ttcgagacca
     2881 gatgggctat ctctttcaaa atttcggcct cttagaaaac caatcaatca aagaaaattt
     2941 ggatttgggt tttgttggtc agaaaatctc aaaagtagaa cgtttggaaa ggcaagtggg
     3001 ggctttagaa aaagttaatc tagggtattt ggatttagaa caaaaaatct atactttatc
     3061 tgggggagag gcccaacgag ttgcccttgc taagactatt ttgaaaaatc cacccttgat
     3121 tttggcagat gaaccaacag cagctcttga tcctgaaaat tcagaggagg ttatgaatct
     3181 cttggtggat ttgaaagatg aaaatcgaat tatcatcatt gcgacccata atcccctagt
     3241 ctggaataag gctgatgaaa tcattgatat gaggaaactt gctcatgtgt gaaaaaatcc
     3301 gtattcgcag ggtatctgat tatcctagtg ccagaggtgg tttagaagat atcctcatca
     3361 tggaaaatat gaccaatcat ctccttttgg ttcaaatccg agtgcatggc tatttgcttg
     3421 attttgctag tattgaaggg caaaggcaaa agcattatcg tttgaaaaat ttacctcaga
     3481 cggttgaact gacagtggat gatgtggagg aggatgtgga tttgacccta cccgaaaatc
     3541 gaagttatca agaagctgat ttttttgata ctcaatgaaa atcaaagagc aaactaggaa
     3601 gctagccgca ggctgtactt gagtacggca aggtgaagct gacgtggttt gaagagattt
     3661 tcgaagagta tgaacgtatg tttcgagaga agcactaagt ccctctttaa agatttccaa
     3721 gactatcttt cttcatgagg aaagatagtt ttttggtatg attttcattc ccaaaataca
     3781 aggggaatgt gttacaatag tagtaacaga taatagaaaa gagaatagat gagaattgca
     3841 gattatagcg tgaccaaggc agtgttggag cgtcacggtt ttacctttaa aaagtccttt
     3901 gggcaaaatt tcttgacgga taccaatatc cttcaaaaaa ttgtggatac ggctgaaatt
     3961 gatgatcagg tcaatgtcat cgaaatcggg ccaggtattg gtgccttgac agaatttttg
     4021 gctgagcgtg cagcccaagt catggctttt gagattgacc accgtttgat gccaattttg
     4081 gcagatactc tgcgtgattt tgataatgtg accgtagtta acgaagatat tctcaaggtt
     4141 gatttggcgc aacatatcca aaattttaaa aatcctaacc tgccaatcaa ggtagtggct
     4201 aatttgcctt actacatcac gacgcctatt ctcatgcact tgattgagag tggcattcct
     4261 ttttgtgagt ttgtggtcat gatgcagaaa gaagtagcgg accgcatttc agcccagcct
     4321 aacaccaagg cttacggtag cttgtctatc gccgtgcagt attacatgac agccaaggtt
     4381 gcctttatcg tgcctcgtac ggtctttgtg ccagcgccaa atgtggattc agccatcttg
     4441 aaaatggtgc gtcgtccaga gccagccgta gcagtagaag atgagaactt tttctttaag
     4501 gtttccaagg ctagttttac ccatcgccgc aagaccttgt ggaataactt gacaggttac
     4561 tttggtaaga ctgaagaggt caaggacaag ctgaccaagg ctttggacca ggcaggcttg
     4621 tcaccaagtg tgcgtgggga agctctcagc ttggcagaat ttgccggtct agcagacgca
     4681 cttaaagggc aaggactcta agatgcaggg acaaatcatt aaagccttgg caggtttcta
     4741 ctatgtggag agtgatggcc aggtttatca aacacgcgcg cgtgggaatt tccgtaaaaa
     4801 aggccatacc ccttatgttg gggactgggt agatttctct gccgaggaaa attcagaagg
     4861 ctatatcctc aaaattcacg aacggaaaaa cagtctggtt cgtccgccta ttgtcaatat
     4921 cgatcaagct gtagtaatca tgtccgtcaa ggaacctgat tttaacagca atttgctgga
     4981 tcgtttcttg gttcttttgg agcacaaggg catccatccc attgtctata tttccaaaat
     5041 ggatttgttg gaagataggg gagaactgga tttttaccag cagacctatg gtgacatcgg
     5101 ctatgacttt gtgaccagta aagaggaact cctgtctttg ttaacaggca aggttacggt
     5161 ctttatgggg cagacaggtg ttgggaagtc aactcttctc aataaaatcg caccagacct
     5221 caatcttgaa acgggagaaa tttcagacag tctaggtcgc ggtcgccata ccactcgagc
     5281 tgttagtttt tacaatctca acgggggtaa aatcgcagat acaccaggct tttcatctct
     5341 ggactatgaa gtatcaaggg ctgaagacct caatcaggcc tttccagaga ttgctactgt
     5401 tagccgagat tgtaagttcc gtacttgtac ccatacccat gagccgtctt gtgccgtcaa
     5461 accagctgtt gaagagggtg ttattgcaac cttccgtttt gacaattacc tgcaattcct
     5521 tagtgaaatt gaaaatcgta gagaaaccta taaaaaagtc agcaaaaaaa ttccaaaata
     5581 aggagaaacc tatgtctcaa tacaagattg ctccgtcaat tctggcagca gattatgcca
     5641 actttgaacg tgaaatcaaa cgtctagaag caactggggc agaatatgcc catatcgata
     5701 tcatggacag tcattttgta ccgcaaatca gttttggtgc aggtgtggtc gagagccttc
     5761 gtcctcatag taagatggtt ttcgattgcc acttgatggt gtcaaaccct gagcatcatc
     5821 tggaagattt tgcgcgtgca ggtgcagaca tcatcagtat ccatgtagaa gcaacgcctc
     5881 atattcatgg cgccctccaa aaaattcgtt cactcggagt taagccttca gtcgttatca
     5941 atcctggcac accagttgaa gccatcaagc acgtccttca tctagttgac caagttttag
     6001 tcatgacggt taatccaggt tttggtgggc aagcctttct gccagaaacc atggataagg
     6061 ttcgtgagtt ggttgctctt cgtgaggaaa aaggtttgaa ctttgaaatc gaagtggatg
     6121 gtgggattga tgaccaaact attgctcaag ccaaagaagc tggtgcgact gtttttgtag
     6181 caggttccta tgtctttaag ggagaagtca atgagcgagt acaaactctc agaaaacaac
     6241 tggactaggg ttgcagtttt tgcaggcgga aaccgcggtc attatcggac agattttgat
     6301 gcttttgttg gggtggatcg aggctcgctc tgggtcttgg aagaagactt acctcttgct
     6361 ctagcagtcg gagattttga ttctgtgacg gaagaagagc gacaggtgat tcaaaaacgt
     6421 gcccagtatt ttgtccaagc ccgaccagaa aaagatgata ccgatttgga attggctctc
     6481 ttaaccatct ttgagaaaaa tcctcaggct caggttacta ttttcggtgc cttgggtggc
     6541 cgtattgacc atatgttggc caatgtcttt ctgcctagca atcctaagtt ggcaccctat
     6601 atgcatcaaa tagaaattga ggatgggcaa aacttgatta cttattgtcc agaagggacc
     6661 agtcagctag aacctcgttc agactacgac tatctagcct ttatgccagt tcgggatagc
     6721 cagctgacta ttcttggagc caagtatgag ttgacagagg aaaatttttt ctttaaaaaa
     6781 gtgtacgctt ctaacgaata tatagatagg gaagtgtcgg taacttgccc agatggttat
     6841 gtggtcgtac tgcatagcaa ggataggagg taggatggaa agtttactta ttctattatt
     6901 aattgccaac ctagctggtc tctttctgat ttggcaaagg caggataggc aggagaaaca
     6961 cttaagtaag agcttggagg atcaggcaga tcatttgtca gaccagttgg attaccgctt
     7021 tgaccaagcc agacaagcca gccagttaga ccaaaaagat ttggaagtgg ctgtcagcga
     7081 ccgtttgcaa gaagtgcgga ttgaattgca ccaaggcctg acccaagtcc gtcaagaaat
     7141 gacagataat ctcctccaaa ctagagataa gacagaccaa cgtctccaag ccttgcagga
     7201 atcaaatgag caacgtttgg aacaaatgcg ccagacggtc gaggaaaaac tagaaaagac
     7261 cttgcagaca cgcttacagg cttcctttga gacagtttct aaacaactgg agtctgtcaa
     7321 tcgtggtctt ggagaaatgc agacagttgc ccgtgatgtc ggagctctta acaaggttct
     7381 ctctggaacc aagacgcgag ggattctggg agaattgcaa ctggggcaaa ttattgaaga
     7441 catcatgaca cctgcccagt acgaacgaga atacgcaacg gttgaaaact ctagtgaacg
     7501 agtggagtat gccatcaagt tacccggaca aggcgaccaa gaatacgtct atcttccaat
     7561 tgactctaag tttccactgg cagattatta ccgcttggaa gaagcctatg agacaggtga
     7621 caaggatgag attgaacgct gtcgtaagtc actcctagca agcgtcaagc gctttgccaa
     7681 ggatatcaag agcaagtaca tagcaccgcc tcggacaacc aactttggaa tcttgtttgt
     7741 tccgacagaa ggtctttatt cagagattgt gcgcaatccg gtcttctttg atgatttgag
     7801 acgggaagaa cagattattg ttgcaggacc aagtacccta tcagcccttc ttaactccct
     7861 atcagttggt ttcaagaccc ttaatatcca aaagagtgcc gaccatatca gcaagactct
     7921 tgccagtgtc aagaccgagt ttggcaagtt tggtggtatt ctggtcaagg cacaaaaaca
     7981 tctccaacat gcctctggca atattgatga attattaaac cgtcgtacca tagctatcga
     8041 gcggacgctc cgtcacattg agttgtcaga aggtgagcct gcgcttgatc tactctattt
     8101 tcaagaaaat gaggaagaat atgaagatta gtcacatgaa aaaagatgag ttatttgaag
     8161 gcttttacct aatcaaatca gctgacctga ggcaaactcg agctgggaaa aactacctag
     8221 cctttacctt ccaagatgat agtggcgaga ttgatgggaa gctctgggat gcccaacctc
     8281 ataacattga ggcctttacc gcaggtaagg ttgtccacat gaaaggacgc cgagaagttt
     8341 ataacaatac ccctcaagtc aatcaaatta ctctccgcct gcctcaagct ggtgaaccca
     8401 atgacccagc tgatttcaag gtcaagtcac cagttgatgt caaggaaatt cgtgactaca
     8461 tgtcgcaaat gattttcaaa attgaaaatc ctgtctggca acggattgtc cgaaatctct
     8521 acaccaagta tgataaggaa ttctactcct atccagccgc caagaccaac caccatgcct
     8581 ttgaaacggg cttggcttat catacggcga ccatggtacg tttggcagac gctattagcg
     8641 aagtttatcc tcagctcaat aagagcttac tctatgcggg gattatgctg catgacttag
     8701 ctaaggttat cgagttgacg gggccagacc agacagagta cacagtgcga ggtaatcttc
     8761 ttggacatat cgctctcatt gatagcgaaa ttaccaagac agttatggaa ctcggcatcg
     8821 atgataccaa ggaagaagtc gttttgcttc gtcatgtcat cctcagtcac cacggcttgc
     8881 ttgaatatgg aagtccagtc cgtccacgta ttatggaggc agagattatc catatgattg
     8941 acaatctgga tgcaagcatg atgatgatgt caacagctct ggctttggtg gataaaggag
     9001 agatgaccaa taaaatcttc gctatggata atcgttcctt ctataaacca gatttagatt
     9061 gataatttaa gaaaaatgag cattttttag gataagaatg ttcgtttttt tatgtgaata
     9121 tggtataata agtaaaagac aaaaatgaat actcttcgaa aatctcttca aactagggta
     9181 gtatcgcctt gtcgtatgta tatatgcagg tatattacag ggtttgtcag ttctatcgac
     9241 aatctcaaag cagtgttttg aaccaccagc gaccagcttt ctagtttgct ttttgatttt
     9301 ttgaataaaa atggaatagg aaatagaaat gaaattaaga agaagtgatc ggatggttgt
     9361 catttccaac tatttgatta ataatcctta taaactaact agtctcaata cttttgctga
     9421 aaagtatgag tctgctaaat catccatctc agaagatatc gtcattatca aacgcgcctt
     9481 tgaggaaatt gaaatcggtc atatccagac agtgactggg gctggcggag gtgtcatctt
     9541 cacaccgtct atttcgagtc aggatgctaa ggaaatggtt gaagacttgc gtaccaagtt
     9601 gtcagaaagt gaccgtatct tgccaggtgg ttatatctat ctgtctgatt tgcttagcac
     9661 accagccatc ttgaaaaata ttggtcgtat tattgccaaa agctttatgg accaaaaaat
     9721 tgacgcggtt atgaccgtag caactaaggg tgtgccactt gcaaatgcag ttgccaatgt
     9781 cctcaatgtc tcttttgtca ttgtgcgccg tgacctgaaa attaccgaag gttcaactgt
     9841 tagcgtcaac tatgtttcag gttcaagtgg tgaccgtatc gagaaaatgt tcctttcaaa
     9901 acgtagtctt aaggcaggta gccgtgtctt gattgtggat gacttcttga aaggtggcgg
     9961 aacggtcaac ggtatgatta gtctcttgcg cgagttcgac tcagaactgg caggtgtagc
    10021 ggtctttgcg gacaatgccc aagaagaacg tgaaaagcag tttgactaca agtcactctt
    10081 gaaggtaact aatattgatg tcaagaacca agccatcgat gttgaggttg gcaatatctt
    10141 tgacgaagat aaataagaga tagaactaaa ggttggaacg attgtcccag cctttctttg
    10201 caaacagaat agaaggaagc ttatgaaaac accatttatc aatagagaag agttagaagc
    10261 gattgttgcc gagttcccga ctccctttca cttgtatgat gagaagggga ttcgtgagaa
    10321 ggcaagagcc gtcaaccaag ctttttcgtg gaacaagggc tttaaggaat attttgcagt
    10381 taaggctact ccaactccag ctattttgaa aattctccaa gaagaaggtt gtggtgtgga
    10441 ctgctctagt tatgtagagc ttttgatgag ccataaactg gactttctgg gttctgagat
    10501 tatgttctct tccaacaaca cgccagacaa ggaatacgcc tatgcacgtg aattgggtgc
    10561 gaccattaac ttggatgcct ttgaagatat tgaacatctg gagagagtag caggcattcc
    10621 agaaatcatc tcttgtcgtt ataatcctgg aggcgttttt gaactgggga cagacattat
    10681 ggacaatcct ggggaggcta agtttggcat gaccaaggac cagctctttg aagcctttgc
    10741 tatcttgaag gaaaaaggag ccaagacttt tgggattcac tccttcctag cgtccaatac
    10801 cgtgacccat ctctattatc cagagttggc tcgtcagctc tttgaactgg ctgttgaaat
    10861 caaggaaaag ttgggcattt ggctagactt tatcaatctt tctggcggta ttggtgttaa
    10921 ttatcgtcca gaccaggagc cgaacgatat cgccttgatt ggtgagggag ttcgtaaggt
    10981 gtatgaagaa gttcttacgt cagcaggtct tggtcaggtc aagattttca ccgaattggg
    11041 tcgttttatg ctggcacctc acggtgctct agtcacaaga gtgactcata agaaggaaac
    11101 ctaccgtacc tatctaggtg tggatgcctc agcagtcaac ctcatgcgtc cagctatgta
    11161 cggagcttac catcatatta gcaacgtgac ccatccagat ggaccagctg aagcggtaga
    11221 tgtggttggt tcactctgtg aaaacaatga taaatttgca gtcaatcgcg aactgcctca
    11281 tacagaaatc ggtgatttgc tggtaattca tgatacaggt gcccacggat tttcaatggg
    11341 ctaccagtat aatgccaaat tacgttctgc ggaaatcctc tataccgaag aaggtaaagc
    11401 ccgtcaaatc cgccgtgcag agcgccctga ggactatttt gcaaccttat atggcttcga
    11461 ttttgaagaa taatctgata atagattgaa aatgaaattg aaaaacagat tgctttctaa
    11521 aaaataggca aaaatcttgt ttttccttca agtcgtgata taataaaact ataaaacgtt
    11581 ttcaaggaag gtaacgatat gtctgaagaa acaattgatt atggacaagt gacaggaatg
    11641 gtgcattcga cagaaagctt tgggtcagta gatgggcctg gtattcgctt tattgtcttt
    11701 ttgcagggct gtcacatgcg ttgccagtat tgccacaacc cagacacttg ggctatggag
    11761 tccaataagt cacgtgaacg gacggtagat gatgtcttga cagaggcctt gcgctaccgt
    11821 ggtttctggg gaaataaggg tgggattaca gtcagtggag gagaagctct cttgcagatt
    11881 gatttcctga ttgctctctt caccaaggct aaggaacaag gaatccactg taccttggat
    11941 acctgtgctc ttcctttccg taataaacca cgttaccttg agaagtttga caaactcatg
    12001 gctgtcactg acttggttct tttggatatc aaggaaatca acgaagaaca gcacaagatt
    12061 gtcactagac aaaccaataa aaatatcttg gcttgtgccc agtatctatc agatattgga
    12121 aaacctgtct ggattcgcca cgtgctagtt ccaggattga cagacagaga tgatgacttg
    12181 attgaacttg gtaagttcgt caagaccctc aaaaatgttg ataagtttga aattctacct
    12241 tatcacacca tgggtgagtt caagtggcgt gaacttggaa ttccatattc cctcgaagga
    12301 gtcaaaccac caacagcaga tcgcgtcaag aacgctaaaa aactcatgga taccgaaagt
    12361 tatcaagatt atatgaaacg tgtacatgga tagaaaagaa gcctgatgga aacatcgggc
    12421 ttttgatttg caaaaagact tagcaaatca gctaagcctt tttcttctta tctcgaacgt
    12481 tgttttccag cgttgcgatt tttgtgtttt ttcttgcttg tgatagcagt tggttgttca
    12541 ggggtaacgt cttttcgtcc acttggttta gagaaagcac ttgcttttgg tgggttcttg
    12601 gctagttctt cacggacttt tttgcgaagt tttggacgaa cgatatagtt gacgataaac
    12661 tgttggagaa tcatcatgaa accaccgaca acccagtaaa gtgtgacact agctggtgag
    12721 aagagggaga agacgacgat catgagtggg ctcatgtaaa tcattttctt gatttgttct
    12781 ctttgcattt catcttctac tccgtgaagt gaaaggagcg attgaagata gtaaaggaca
    12841 ccagcacagg caaccaaaat catacttgga gaacctagag gaatgcctag gtagcttgct
    12901 tgagcaaccc cttcagtatg ttgggcagca aagtagatag cagagaagaa aggcatttga
    12961 aggaggatag ggaaacatcc tacaccgcca aacatgctga taccgtgctc tttttgagca
    13021 gcaaagagag cttgttgggc ttcgagtttt tcttcttgag tagtcgcttc tttgagacgc
    13081 gtttggtgtg gctcaaggac gtgcttgagg gcgttcatct tttcagagtg aagcgttgcc
    13141 ttccatgatt ggtagatacc aagtggcaag ataatcaagc gcacgataat ggttacgatg
    13201 atgatagcga caccaaagcc tagaccttta tcagtagcga agtacttgat agcttcagcc
    13261 ataggggctc cgatggtatt ccaaataaat cctgttggct gacctgtggt tttatcgaca
    13321 ttgacacaac cagtcaagac aagcaacata gccgctccca tagctgagag tgcaaaacgt
    13381 ttaatagatt tcactgtttt tattccttct ttaaaaatta tacctttcta ttctactgtt
    13441 tttttacaaa atatacaata gttctggaga cctaatttgc gattttaaag tcagagtagg
    13501 gaggaaagtt gcttagtttg acatctaagt agcttacttt tgaaaaaggt gtcggtcctt
    13561 ttcggatttc ttggataaat tttgccatga tagcagatga gtctgcttgg gctaagattt
    13621 ccactgtgcc atcgtcgtta ttccatactc gacctgtgat gccaccaatt tcaagtgcca
    13681 agctgtaaac accccaacga aagccgactc cctgtaccct accttgggca atcattctaa
    13741 ccttttgcat accaaacccc tttgattgtg ttataatatt tctatgacta ttataacctc
    13801 aaaagccaat tctgtggtaa aaaatgccaa gaaattacgc caaaaaaaat accgcaagtc
    13861 tgcctacttg attgaaggct ggcacttgtt tgaagaagct gtccaagctg gagtgacgat
    13921 tgagaaaatc tttgccctag aaaattatcg agatcagtta gctacttttt cgcaaactgt
    13981 ctgggtgtca gaggatattt tgctagattt ggcggattct cagactccac agggaattgt
    14041 tgccgtggtt caaaaagaag aagtaggaca agctgatttg agtcagggca agttcttgtt
    14101 tttggaagat gtgcaagatc ctggtaatgt aggaactatc attcgaactg cggatgcagc
    14161 aggttttaca ggagtgattg tttcagataa gtcggcagac atctacagtc tcaaaaccct
    14221 acgttccatg caaggtagtc atttccatct gcccatttac cggatgacta gtcaagcgct
    14281 tcttgacgaa actaaaaagg tagctatccc agtgctagca acaaccctat ctaaagattc
    14341 tgttgattac agagaactgc cttctataga aaattttgta ctagttatgg gaaatgaggg
    14401 tcaaggaatt agtcccctta tggctgaaag tgcagaccag ttggtccata ttagcatgaa
    14461 ggggcaggcc gagagtttga atgttgcggt tgcagccggt attttaatct tccatttaag
    14521 ctaattttaa ctttttttgt tataatcaag gaaagatgtt cacagaaaag gagaaatgga
    14581 tgaatcacac tattatacat gaccgtgcag gtctcaatca attttacgct aaggtttatg
    14641 cctttgttgg tctgggaatc ggactatctg ctttggtatc aggccttatg ttgacggtct
    14701 ttcagtctca gttggtttac tttttgatgc aggggcgtct ctggttgacc attgctactt
    14761 ttgcggagtt ggctttggtt ttcgttgcca gtagcatggc ttcaagaaat agtccagctg
    14821 ctcttccagt atttttactt tactccgttt taaacggctt tacccttagt tttgtggtgg
    14881 ctttctatac tccgggtaca gttttatctg cctttgtatc gagcgccctt ctcttctttg
    14941 tcatggcggc agttggtatt tttaccaaga aagacttgag tgggattggt cgagcaatga
    15001 tggcagctct cattggttta ctgattgcta tggtagtcaa tattttcttg gctagtggct
    15061 tctttgatta tatgattagc gtagccatgg ttttggtttt ttcagggttg attgcttggg
    15121 ataaccaaaa gattcgcctc gcctatgaac aatcacaagg tcgtgtagcg acaggctggg
    15181 tggtgtcaat ggctctcagc atctatcttg attttatcaa tcttttccta agtattctac
    15241 gtatctttgg tcgcaatgac taaaaggtct atgaaatcag tgttttgagt gacactgatt
    15301 ttttttaaat tttctctttt cttttcttgg aaataggtgt ataatacgtt tattaaattt
    15361 ttgaggagtt gtctatgaag aaaagtttta tccatcaaca agaagaaatt tcctttgtca
    15421 aaaacacttt tacccagtat ttgaaagata agctagaagt tgtcgaagtt caaggtccta
    15481 tcttgagtaa ggtcggtgac ggaatgcagg acaacctgtc tggtgtggaa aatccagtat
    15541 cggtcaaggt tctccaaatc cctgatgcta cttatgaagt ggtgcactca cttgctaaat
    15601 ggaaacgcca caccttggct cgttttggct ttggtgaagg agagggtctc tttgtccaca
    15661 tgaaagccct tcgtccagat gaggattcct tggatgcaac ccactctgtt tatgttgacc
    15721 agtgggactg ggagaaggtt atcccaaatg gtaagcgtaa catcgtttat ctaaaagaaa
    15781 cagttgagaa gatttataag gctattcgcc tgactgagct agctgttgaa gcccgctatg
    15841 acatcgagtc tatcttgcca aaacaaatca cctttatcca tacagaagaa ttggtagaac
    15901 gctacccaga cttgacacct aaagaacgtg aaaatgcgat ttgtaaagaa tttggagccg
    15961 tctttttgat tggtatcggt ggcgagttgc cagatggtaa accgcacgat ggacgtgcac
    16021 cagactatga tgactggaca agcgagtctg agaatggcta caagggtcta aatggtgata
    16081 ttcttgtctg gaatgagtct ttaggtggag cctttgagtt gtcttctatg ggaattcgtg
    16141 tagatgaaga aactcttaga cgtcaggtgg aaattacagg tgatgaagac cgcttggaat
    16201 tggaatggca caagtctttg ttgaatggcc tattcccatt gacaatcggt ggaggaattg
    16261 gacaatctcg tatggccatg ttcctacttc gcaagagaca catcggagaa gtgcaaacaa
    16321 gtgtttggcc tcaagaagtc cgcgatactt acgaaaatat tttgtagaga atcgaaccgc
    16381 aaggttcggt tttctttctc tttttgtcta taatttggta taataaacag tatgaaaatc
    16441 gtatcaggaa tctatggggg acgtcccctc aagacactag aaggcaagac aacaagacct
    16501 acttcggata aggttagggg agccattttt aacatgattg gtccctactt tgaaggggga
    16561 cgagtcttgg acctttatgc aggtagtggt ggtttatcta tcgaagcagt atcgcgtggc
    16621 atgtccagtg ctgttttggt ggagcgagac cgtaaggctc agaccatcgt ggctgaaaat
    16681 atccagatga ccaaggaagt tggaaaattt caactcctca agatggatgc agagagggca
    16741 ttggaacagg tatctgggga atttgacctc gttttcttag accctcccta tgccaaggaa
    16801 caaatcgtag cagatattga aaaaatggct aagagagagc ttttttctga agatgttatg
    16861 gttgtgtgcg agacggataa ggccgttgaa cttccagaag agattgcctg tctgggtatc
    16921 tggaaggaaa agatttatgg aattagtaag gtgacagtct atgtcagata agattggctt
    16981 attcacaggc tcatttgatc cgatgacaaa tgggcatctg gatatcattg aacgggcgag
    17041 cagacttttt gataagcttt atgtgggtat tttttttaat ccccacaaac aaggatttct
    17101 ccctcttgaa aatcgtaaac gggggctaga aaaggctttg ggacatttgg aaaatgttga
    17161 agtcgtggct tctcatgatg aattggtggt cgatgttgca aaaagactgg gggctacttt
    17221 cctagtacgg gggttgagga atgcggcgga tttgcaatat gaagctagtt ttgattacta
    17281 caatcatcag ctgtcttctg atatagagac tatttattta catagccggc ctgaacatct
    17341 ctatatcagt tcatcaggtg ttcgagagct tttgaagttt ggtcaggata ttgcctgcca
    17401 tgttcccgag agtattttgg aggaaataag aaatgaaaaa aaagattaga tggcccttat
    17461 acgtcattgc ggccttgatt gtgactttct tggcatttgt agtgcccttg ccttattata
    17521 tagaggttcc aggtggttcg gaagatattc gccaagtcct taaagtaaat gacacagaag
    17581 ataaggaagc tggtgcctat caattcgtta cggttggtgt tcaacatgcc actttagctc
    17641 atatgattta tgcttggttg acacctttta cagatattcg tagtgctcag gagactacag
    17701 gtggttcttc cgatgttgaa tttatgcgaa tcaatcaatt ctacatgcaa acatcgcaaa
    17761 atatggccaa gtatcaagga ctaaaaacag ctggtaagga tatcgaactc aagtactttg
    17821 gagtttatgt tttgaatgtg acggataatt caacctttaa agggattctc aatatctctg
    17881 atacagtcac agcagtcaat gatcagacct ttgatagttc caaagacttg attgattacg
    17941 tcagttctca aaaattaggg gattccgtca aggtcaccta tgaagaggat gggcaaacca
    18001 agtctgcaga aggaaagatt atcaccttgg aaaatggcaa aaatggaatt ggaatcggct
    18061 tgattgaccg tacagaggta atcagcaatg tcccaattag cttttcaaca gctggtattg
    18121 gcggcccaag tgctggtctc atgtttagtc tagctatcta tactcaaata gctcacccag
    18181 atcttcgtaa tggtcgtatt gttgccggta caggtaccat tgaccgcgat ggtaatgtgg
    18241 gagacattgg aggtattgat aagaaggttg tagcttcggc tagggcaggt gctgctattt
    18301 tctttgctcc tgataaccct gttagcgaag aagaacaaaa ggcgcatctg gacgcgaaaa
    18361 acaactacca aacagcccta gaagcggcta aaacaatcaa gacggatatg aaaatcgtgc
    18421 ccgttaaaac cctacaagat gcgattgatt acttgaaaaa caatccctaa tttttgagtg
    18481 aagtttccaa actagcgcac aaacagagaa gatggtataa tagtcaaatg gttcaattat
    18541 tattcactct aagtagtcat atgctcttta tttatgtgag tttttacctt ttaaaggatc
    18601 ttgttagatg ggagaaggtt ttaaaagtga cagatgataa tacaagaaaa gttcgtttat
    18661 tagtagcctt ttttagcatt gtcataggct acatcctgag ttctttcttt attagcctgt
    18721 atcatttgtg gcaagaagcg cttagaggat tattataaaa tcaagagtaa aggaaacgag
    18781 tatggataaa attgtggttc aaggtggcga taatcgtctg gtaggaagcg tgacgatcga
    18841 gggagcaaaa aatgcagtct tacccttgtt ggcagcgact attctagcaa gtgaaggaaa
    18901 gaccgtcttg cagaatgttc cgattttgtc ggatgtcttt attatgaatc aggtagttgg
    18961 tggtttgaat gccaaggttg actttgatga ggaagctcat cttgtcaagg tggatgctac
    19021 tggcgacatc actgaggaag ccccttacaa gtatgtcagc aagatgcgcg cctccatcgt
    19081 tgtattaggg ccaatccttg cccgtgtggg tcatgccaag gtatccatgc caggtggttg
    19141 tacgattggt agccgtccta ttgatcttca tttgaaaggt ctggaagcta tgggggttaa
    19201 gattagtcag acagctggtt acatcgaagc caaggcagaa cgcttgcatg gcgctcatat
    19261 ctatatggac tttccaagtg ttggtgcaac gcagaacttg atgatggcag cgactctggc
    19321 tgatggggtg acagtgattg agaatgctgc gcgtgagcct gagattgttg acttagccat
    19381 tctccttaat gaaatgggag ccaaggtaaa aggtgctggt acagagacta taaccattac
    19441 tggtgttgag aaacttcatg gtacgactca caatgtagtc caagaccgta tcgaagcagg
    19501 aacctttatg gtagctgctg ccatgactgg tggtgatgtc ttgattcgag acgctgtctg
    19561 ggagcacaac cgtcccttga ttgccaagtt acttgaaatg ggtgttgaag taattgaaga
    19621 agacgaagga attcgtgttc gttctcaact agaaaatcta aaagctgttc atgtgaaaac
    19681 cttgccccac ccaggatttc caacagatat gcaggctcaa tttacagcct tgatgacagt
    19741 tgcaaaaggc gaatcaacca tggtggagac agttttcgaa aatcgtttcc aacacctaga
    19801 agagatgcgc cgcatgggct tgcattctga gattatccgt gatacagctc gtattgttgg
    19861 tggacagcct ttgcagggag cagaagttct ttcaactgac cttcgtgcca gtgcagcctt
    19921 gattttgaca ggtttggtag cacagggaga aactgtggtc ggtaaattgg ttcacttgga
    19981 tagaggttac tacggtttcc atgagaagtt ggcgcagcta ggtgctaaga ttcagcggat
    20041 tgaggcaagc gatgaagatg aataagaaat caagctacgt aatcaatcgt ttacttttag
    20101 tcatcatagt actgatttta ggtactctgg ctctaggaat cggtctaatg gtaggttatg
    20161 gaatcttggg caagggtcaa gatccatggg ctatcctgtc tccagcaaaa tggcaggaat
    20221 tgattcataa atttacagga aattaggctg gagaaccagc ctttttctaa agataaggag
    20281 aaatatgaac aaaaaaacaa gacagacact aatcggactg ctagtgttat tgcttttgtc
    20341 tacagggagc tattatatca agcagatgca gtcggcacct aatagtccca aaaccaatct
    20401 tagtcagaaa aaacaagcgt ctgaagctcc tagtcaagca ttggcagaga gtgtcttaac
    20461 agacgcagtc aagagtcaaa taaaggggag tctggagtgg aatggctcag gtgcttttat
    20521 cgtcaatggt aataaaacaa atctagatgc caaggtttca agtaagccct acgctgacaa
    20581 taaaacaaag acagtgggca aggaaactgt tccaaccgta gctaatgccc tcttgtctaa
    20641 ggccactcgt cagtacaaga atcgtaaaga aactgggaat ggttcaactt cttggactcc
    20701 tccaggttgg catcaggtca agaatctaaa gggctcttat acccatgcag tcgatagagg
    20761 tcatttgtta ggctatgcct taatcggtgg tttggatggt tttgatgcct caacaagcaa
    20821 tcctaaaaac attgctgttc agacagcctg ggcaaatcag gcacaagccg agtattcgac
    20881 tggtcaaaac tactatgaaa gcaaggtgcg taaagccttg gaccaaaaca agcgtgtccg
    20941 ttaccgtgta accctttact acgcttcaaa cgaggattta gttccctcag cttcacagat
    21001 tgaagccaag tcttcggatg gagaattgga attcaatgtt ctagttccca atgttcaaaa
    21061 gggacttcaa ctggattacc gaactggaga agtaactgta actcagtaaa agataagcct
    21121 aaactcctat gtcacttatg gatgtaggag ttctttttac tagtttaagc agggctagaa
    21181 caggtactaa gaaaaaatag taacttctaa aactaacttc cagtttccca caacctagct
    21241 tttagtatga gaaaaacgcg tgaaatcagt ggaaattcgt cttagactaa cttccagttt
    21301 tgggagagag atggaagtta cgctaagaaa aaaataatat gtattggatt aaattttgtg
    21361 atataataaa ggataagata ctattttagg agaagaacta tggaagaccc aagtagtcag
    21421 aatttgttgc tacaatttgt tttattgttt atcttgacgg tattaaatgc ctttttctca
    21481 gccacagaaa tggccatggt ttctcttaat cgtgcccgag ttgaacaaaa ggcggaagaa
    21541 ggagacagac gctacatccg cctgctgaag gtactagaaa atcctaacca ctttttatca
    21601 accattcagg taggaatcac cctgattaca atcttatcag gggcgagttt aactgatact
    21661 ctaggacgtg tgattgcctc ttggcttggg aatggcgaaa cagcacaagc cgtggcaact
    21721 tttctatcct tggcattttt gacctatatt tccatcgttt ttggggaatt atatcctaag
    21781 agaatcgctc ttaatctaaa ggatgccttg gcaattcgta cagcgccggt tatcattggg
    21841 atcggaaaac tagtcagtcc ttttgtttgg cttttagctg cttcgaccaa tttcttgagc
    21901 catttgactc ctatgtcatt cgatgatgcg gatgaaaaga tgacccgtga tgaaattgcc
    21961 tatatgctga caaatagtga agaaacattg gatgctgatg agattgagat gctacaaggt
    22021 gtcttttcgc tcgatgaact gatggcacga gaggttatgg ttcctcgaac ggatgccttt
    22081 atggtggata ttcaggatga tagtcaagcc attatccaaa gtattttaaa acaaaattat
    22141 tctcgtatcc cggtttatga tggggataag gacaatgtaa ttggaatcat tcacaccaag
    22201 agtctcctta aggcaggctt tgtggacggt tttgacaata ttgtttggaa gagaatttta
    22261 caagatccac tttttgtacc tgaaactatt tttgtggatg acttgctaaa agaactgcga
    22321 aatacccaaa gacaaatggc aatcttgctg gatgaatacg gtggtatggc tggtttggtg
    22381 actttggaag acctcttaga ggaaattgtt ggtgaaattg atgatgaaac ggacaaagca
    22441 gcaatagatg ttcatcagat tggcgaggat acctacattg ttcaaggaac tatgaccctc
    22501 aatgatttca ataattactt taatgttgaa ctggaaagtg atgacgttga tactatcgct
    22561 ggttattatt tgacaggagt gggaaccatt ccaacgactg agaaactcag ctatgaattg
    22621 atcagccaaa acaaacagtt tatcttaacc aatgataaag tgaagaatgg acgtgttacc
    22681 aaggtaaaaa tccaaatcac agaagtcgaa atagaagaag aaacagaata aactagaggc
    22741 ttttgtcacc aggcgtgacg aagctttttt caattgattt caaaagggaa ttatgccatg
    22801 gaaagaactc tgccacaaac tcgctccaaa agtttttaaa gttatacgta tttattcgag
    22861 ggaaaataag aaaagccctt caaattgggc tttctgttca tttgaaacct gataaatcaa
    22921 gttatagaag gcggtagacg gatttgaacc gacgatcaag cttttgcaga gccgtgcctt
    22981 accacttggc tataccgcct taacgtttat tattatacct tgaaaatgat tttcagtcaa
    23041 tagttttttc agttttttgt accatagcgt gattcgaatg tgatgccaag ttttctatgg
    23101 gaaaaaattt accaaaaacc tactgaaacc ccttgaaaaa tctagctaaa tagggtataa
    23161 tatgggtaat catttgtcgt aggttttgtc tgaaatattg tccagacaag gctcacagca
    23221 gttaaatctt ctgaaaaagt cagatttagc tgctcttttt gtgctttttt tcaagatttt
    23281 gagcatttgt aacagaggct taaagattct gaaaattcgt caagaggaca cggtgataag
    23341 gggtttacaa ccatatggcg attagaaaag cctgattgac aaggcttgga acttatttac
    23401 aaaggagaat catcttggca ggacatgacg ttcaatacgg gaaacatcgt acccgtcgta
    23461 gtttttcaag aatcaaagaa gttcttgact taccaaattt gattgaaatt caaactgact
    23521 cattcaaagc tttcctagac cacggtctta aggaagtgtt tgaagatgta ttgccaattt
    23581 caaacttcac agacacaatg gagttggaat ttgttggata tgaaatcaag gaaccaaaat
    23641 acacgctaga agaagctcgt atccacgatg ctagctactc agcaccaatt tttgtaacct
    23701 tccgtttgat caataaagaa acaggcgaaa tcaagaccca agaagttttc tttggtgatt
    23761 tcccaatcat gacagaaatg ggtactttca tcatcaatgg tggtgaacgt attatcgttt
    23821 ctcagttggt ccgctcacca ggtgtttact ttaacgacaa agtagacaaa aatggtaagg
    23881 tgggctatgg ttcaactgtt atccctaacc gtggagcttg gttggaactt gaaagcgact
    23941 caaaagatat cacctacact cgtatcgacc gtactcgtaa gattccattt acaaccttgg
    24001 ttcgtgctct tggtttctca ggtgatgatg aaatctttga tatctttggt gacagcgaat
    24061 tggttcgcaa cactgttgaa aaagatatcc acaagaatcc aatggactct cgtacagacg
    24121 aagccttgaa agaaatttac gaacgccttc gtccaggtga gcctaagacg gctgaaagct
    24181 cacgtagctt gcttgtagct cgcttctttg acccacgtcg ttatgacttg gcagcagttg
    24241 gtcgttacaa aatcaataaa aaactcaatg ttaaaacacg tttgctcaac caaaccattg
    24301 cagagccatt ggtagaccct gaaactggag aaatcttggt agaagctggt acgattatga
    24361 ctcgtagcgt gattgaaagc attgaaagcc atttggatgg cgacttgaac aagattgtct
    24421 acatcccaaa cgatgcagcc gttgtgactg agcctgttgt tcttcaaaaa ttcaaggtta
    24481 ttgctccaac tgatccagat cgcgtcgtaa cgatcattgg taatgctaac ccagatgaca
    24541 aggttcgtac ggtgactcct gcagatatcc ttgctgagat gagctacttc ctcaacttgg
    24601 ctgaaggact tggccgtgta gatgatatcg accaccttgg aaatcgtcgt atccgtgcgg
    24661 ttggtgaatt gcttgccaac caagtacgtt tgggactttc tcgtatggaa cgtaatgtcc
    24721 gtgaacgtat gtctgttcag gacaatgaag tcttgacacc acaacaaatt atcaatattc
    24781 gtcctgtaac agctgcagtt aaagaattct ttggttcatc acagttgtca cagttcatgg
    24841 accaacacaa cccgctttct gagttgtctc acaaacgccg tttgtcagcc ttaggacctg
    24901 gtggtttgac tcgtgaccgt gccggatatg aagtgcgtga cgtgcactac actcactatg
    24961 gtcgtatgtg tccaatcgag acacctgaag gacctaacat cggtttgatc aataacttgt
    25021 catcttacgg acacttgaac aaatatggtt ttgttcaaac accataccgt aaggttgacc
    25081 gtgaaacagg tgttgtcacg aacgaaattg tttggttgac agctgatgaa gaagatgaat
    25141 atactgtagc tcaggctaac tctcgtctga atgaagatgg aacctttgct gagaagattg
    25201 tcatgggacg tcaccaaggg gtcaaccaag agtatccagc taatattgtt gactacatgg
    25261 acgtttcacc aaaacaggta gttgccgttg cgacagcatg tattcctttc ttggaaaacg
    25321 atgactccaa ccgtgccctc atgggagcca atatgcaacg tcaggctgtg ccattgatta
    25381 atcctcaggc accttacgtt ggtactggta tggaatacca agcagcccac gattctggtg
    25441 cggctgtgat tgctcagtat gatggtaaag ttacttacgc agatgctgac aaggtagaag
    25501 ttcgtcgtga agatggttca ttggatgttt accacatcca aaaattccgt cgttcaaact
    25561 caggtactgc ttacaaccaa cgcactctcg taaaagttgg tgatgtcgtt gaaaaaggcg
    25621 atttcatcgc tgacggacct tctatggaaa atggagaaat ggcgcttgga caaaacccaa
    25681 tcgttgccta catgacttgg gaaggctaca acttcgagga tgccgttatc atgagcgaac
    25741 gcttggtgaa ggacgatgtc tacacatctg ttcaccttga agaatacgaa tcagaaacgc
    25801 gcgatacaaa gcttgggcct gaagaaatca ctcgcgaaat tccaaacgtt ggtgaagatg
    25861 ccctcaaaga ccttgacgaa atggggatta tccgtattgg tgctgaggtt aaagaaggtg
    25921 atattcttgt agggaaagta acacctaagg gtgagaaaga tctttcagct gaagaacgtc
    25981 tcttgcacgc tatctttgga gacaagtctc gtgaagtgcg tgatacttct cttcgtgtac
    26041 cacacggtgc cgatggtgtc gttcgtgatg ttaagatctt tacacgtgta aatggagatg
    26101 agttgcaatc aggtgttaac atgttggttc gtgtttacat cgctcaaaaa cgtaagatta
    26161 aggtcggaga taaaatggcc ggacgtcacg gaaacaaagg ggttgtctct cgtatcgttc
    26221 ctgtagaaga catgccttac cttccagacg gaactccagt cgacatcatg ttgaacccac
    26281 ttggggtgcc atcacgtatg aatatcggtc aggttatgga gcttcacctt ggtatggcag
    26341 ctcgtactct tggtattcac attgcgacac cagtctttga tggagcaagt tctgaagatc
    26401 tttggtcaac tgttaaagaa gcaggtatgg atagcgatgc caaaacgatc ctttacgatg
    26461 gacgtactgg tgaaccattt gataaccgtg tttctgttgg agtcatgtac atgatcaaac
    26521 tccaccacat ggttgacgat aaattgcacg cgcgttcagt cggaccttac tcaactgtta
    26581 cccaacaacc actcggaggt aaagctcagt ttggtggaca acgtttcggt gagatggagg
    26641 tttgggctct tgaagcctac ggtgcgtcaa atgtccttca agaaatcttg acttacaagt
    26701 ctgacgatat caacggacgt ttgaaagcct atgaagctat tacaaaaggc aaaccaattc
    26761 caaaaccagg tgttccagaa tccttccgag ttcttgttaa agaattgcaa tctcttggtc
    26821 ttgacatgcg tgtcctagac gaagatgacc aagaagtgga acttcgcgac ttggatgaag
    26881 gaatggacga agatgtcatc cacgtagatg accttgaaaa agcccgcgaa aaagcagccc
    26941 aagaggctaa agcagccttt gaagctgaag aagctgagaa agcaacaaaa gcggaagcaa
    27001 cagaagaagc tgctgaacaa gaataagcag ttcacttaga atagaaaggg aagaaatagt
    27061 ggttgatgta aatcgtttta aaagtatgca aatcacccta gcttctccaa gtaaagtccg
    27121 ttcatggtct tatggagaag tcaaaaaacc tgaaacaatc aattatcgta ccttgaaacc
    27181 agaacgtgaa ggactctttg atgaagtgat ctttggtcct acaaaagact gggaatgtgc
    27241 ttgtggtaag tacaaacgca ttcgttacag aggaattgtt tgtgaccgct gtggggttga
    27301 agtaacgcgt acgaaagttc gtcgtgagcg tatgggacat atcgaattga aagctcctgt
    27361 atctcacatc tggtacttca aggggattcc aagccgtatg ggcttgaccc ttgatatgag
    27421 ccctcgtgcc ctcgaggaag ttatctactt tgcggcttat gtggtgattg atcctaagga
    27481 tacaccactt gagcacaagt ctatcatgac agagcgcgaa taccgagagc gcttgcgcga
    27541 atatggttat ggttcatttg ttgccaagat gggtgcggaa gccatccaag accttttgaa
    27601 gcaagtagat cttgaaaaag aaattgctga actcaaagaa gaattgaaaa cagctactgg
    27661 acaaaaacgt gtcaaagcca tccgtcgttt ggatgttttg gatgcctttt acaagtctgg
    27721 aaacaaacct gaatggatga ttcttaacat ccttccggtt atcccaccag atcttcgtcc
    27781 aatgttgcag ttggatggtg gccgttttgc ctcatctgac ttgaatgacc tttaccgccg
    27841 tgttatcaat cgtaacaacc gtttggctcg tttgcttgag ttaaatgcac caggtatcat
    27901 cgttcaaaat gagaaacgta tgcttcaaga agcagttgac gctttgattg acaatggtcg
    27961 tcgtggtcgt ccaatcacag gaccaggtag ccgtccattg aaatcattga gccacatgct
    28021 taaaggtaaa caaggacgtt tccgtcaaaa cttgctcggt aaacgtgttg acttctcagg
    28081 acgttcagtt atcgccgttg gtccaactct taagatgtac caatgtggtg tgccacgtga
    28141 aatggcgatt gaactcttta aaccatttgt catgcgtgaa atcgttgccc gtgatatcgt
    28201 gcaaaacgtc aaagcagcta aacgcttggt ggaacgcgga gatgagcgta tctgggatat
    28261 ccttgaagaa gtgattaaag aacacccagt gcttttgaac cgcgcaccga cccttcaccg
    28321 tttgggtatc caagccttcg agccagtctt gattgatggt aaggctcttc gcttgcaccc
    28381 acttgtctgt gaagcctaca atgctgactt tgacggggac caaatggcca tccacgtacc
    28441 actttcagaa gaagcacaag cagaagctcg tatcctcatg ctagctgctg agcacatctt
    28501 gaacccgaaa gatgggaaac cggtagttac tccatctcag gacatggttt tgggtaacta
    28561 ctacttgacc atggaagaag ctggtcgcga aggtgaagga atggtcttca aagaccgtga
    28621 cgaagcggtt atggcttacc gcaatggtta tgttcacctc cactcacgtg ttggtatcgc
    28681 aacagacagc ctcaacaagc cttggacaga agagcaaaga cataaggtct tgcttacaac
    28741 agttggtaaa attctcttca acgatatcat gccagagggg ctaccatact tgcaagaacc
    28801 aaacaatgcc aacttgacag aaggtgttcc agctaaatac ttcttgccac ttggtggaga
    28861 tatcaaggaa gctatcagca atcttgagct caaccctcca ttcaagaaga aaaacctcgg
    28921 aaatatcatc gctgaaatct tcaaacgttt ccgtacgaca gaaacttctg ccctacttga
    28981 ccgcatgaag aacctcggtt accaccactc aactcttgca ggattgacag tgggtattgc
    29041 cgatatccca gtcgttgatg acaaggctga aatcattgaa gaatcacaca aacgtgtcga
    29101 acaaatcacc aaacaattcc gtcgtggtat gatcacagac gacgagcgtt acaatgctgt
    29161 tacagctgaa tggcgtgctg cccgtgaaaa acttgagaaa cgcttgattg ctaatcaaga
    29221 tcctaagaac ccaatcgtta tgatgatgga ctctggagcc cgtggtaaca tctcaaactt
    29281 ctcacagctt gccggtatgc gtggtctgat ggctgctccg aatggacgta tcatggaatt
    29341 gccaatcctt tcaaacttcc gcgaaggttt gtcggtattg gaaatgttct tctcaactca
    29401 cggtgctcgt aaaggtatga ccgatacggc ccttaagaca gccgactcag gttacttgac
    29461 tcgtcgtttg gtcgacgttg cccaagacgt tatcatccgt gaggacgact gtggaactga
    29521 ccgtggtctc ttgatccgtt ctatcgcaga aggaaaagag atgatcgagt ctctcgaaga
    29581 gcgtctcaac ggtcgttaca ctaagaaaac tgttaaacat ccagaaactg gtgcagtgat
    29641 tattggtcca aatgaattga ttacagaaga caaggcgcgt gaaattgtca atgctggtgt
    29701 ggaagaagtg actatccgtt ctgtatttac atgtaacact cgtcacggtg tctgccgtca
    29761 ctgttacggt atcaacttgg cgactggtga tgcggttgaa gttggtgaag cagttggtac
    29821 aatcgctgcc caatctatcg gggaacctgg tacacagctt acaatgcgta ccttccacac
    29881 aggtggggtt gcctcaaata ccgatatcac tcagggtctt cctcgtgtcc aagaaatctt
    29941 tgaagcccgc aatcctaaag gggaagcggt tatcacagag gttaaaggac aagttactgc
    30001 tatcgaagag gatgcatcaa ctcgtaccaa gaaagtcttt gttaagggtg aaactggcga
    30061 aggtgaatat gtcgttccat ttacagctcg tatgcgtgtc gaagttgggg gccaagtagc
    30121 gcgtggtgct gctctgacag aaggttctat ccaaccaaaa cgtctccttg cagttcgtga
    30181 tgtcttgtca gttgaaacgt accttctcgg tgaagtacaa aaagtttacc gtagccaagg
    30241 ggtagaaatc ggtgacaaac acatcgaggt aatggttcgt caaatgatcc gtaaagtccg
    30301 tgtcatggat ccaggtgata cagatcttct catgggtacc ctcatggata tcaatgactt
    30361 tacagatgcc aacaaagatg tccttatcgc aggtggagtt ccagcgacag gtcgcccagt
    30421 ccttatggga attaccaaag cctcacttga aacaaacagt ttcttgtcag cggcttcctt
    30481 ccaggaaaca actcgtgtcc ttactgacgc agctatccgt ggtaagaaag accatctcct
    30541 tggacttaaa gaaaatgtta tcatcggtaa gatcatccca gctggtactg gtatggcccg
    30601 ttaccgtaac cttgaaccac atgctgtcaa cgaagaagaa taccttaatc ctccagtaga
    30661 ggaagaagga aatgaagaaa caacagaagt agttgtggat actgccgttg aaactgtgga
    30721 agaaacagta gaataagaga gaatgagaga accctcgggt tctctcattc tctcttctga
    30781 aaactttctc catttttcca cgaaatgtgg taaaataaaa gaaagaagct gacaaaggag
    30841 acgggtatgg aacaaacatt ctttatcatc aaaccagatg gtgtaaaaag agggctagtg
    30901 ggtgaagtgt taaagcgcat cgaacaacgt ggatttacaa tcgaaaaatt ggagtttcgt
    30961 tcacaggttt cagaagagtt gattgaccag cactatcagg acttggttgg tcagagtttt
    31021 tacccaccga ttcgtgaatt catgacttca ggtccagttc ttgtgggtgt catttctggt
    31081 cccaaagtaa tcgaaacttg gcggaccatg atgggtgcaa ctcgtccaga agaagcttta
    31141 ccaggcacta ttcgaggtga ttttgcaaaa gctgctggag aaaatgagat tatccaaaat
    31201 gttgtacatg gttcagattc agaagagtca gctaagcgag aaattgctct ttggttttaa
    31261 gagtggattg gctcaatcaa ttggttaaaa gctcatttga atagaaagta tagtcaatta
    31321 gtttaagaca tgacgcatga tatcaaactt tttagttttt gatatggtgc gttttttgat
    31381 taagtgacta ttagtttgtc tcgctccgtt aggtgcgaga caaaaaaaac acccgctatg
    31441 cgggtgcgcg tcgaaggtta taccaaaaaa actccaaacg cgatacaata aaggtgttca
    31501 atccaattgt aaagcgaaag gagaaaaata tggcacaaaa ggttctttta tcctgttctt
    31561 gtttcaattg actatatttc tatacttttt cttcctttct ctgtgaaaat cgcaagtttt
    31621 tcccaccttt tctcaatact gaaacaacat ctttccagaa cttcaataat ttctttttgt
    31681 gttaaatcat tatttaacta aatctttgga ggcaatacgt tttcaaagct acttttttac
    31741 cataggtttt gagccttcaa cacagattac taagccaaaa atgctctaaa tttttgaaat
    31801 tctaacaaat tccactcaac tttatcatca tctttgactt tcacaatatc ttcctcaaaa
    31861 taacatggaa taaagatagt tttcttttaa aacggttgta ggaggaaaat ctctcctaca
    31921 ctattattga ttctgagaga aaattgcaaa atatcatcta ccaaactttc aattattaag
    31981 attttttgca gtttatctga attagaatcg caaggcttct caataatata attgtcgaat
    32041 tttttcattc taacttcctc tccattattt cttcctttat caagaaaaag ttacctcaat
    32101 attaataaag tacaattcta aattaactcc aattcttgat tcatgataat atttcagtaa
    32161 ctcatcgtgc aattaaaaac aaggattttt atttcagatg atccatcgtg aacacttcat
    32221 cagccatctc ccaaattgcc ggattatgtg ttgcgataat gattagccta ttatcatctc
    32281 gaagagatag caaaatctcc ataatcaact gagaggttgc tgggtctatt gaagctgttg
    32341 gctcatctgc cagaataaag ggtggattct ttaagataat ttttgccaag gcaacccgtt
    32401 gcgattctcc gcccgataac tcaaagatgc gcttatctag gtcaagataa gccaggccga
    32461 cctgttctaa agcctgcttc tgcctcaacc gctgttccga ccgactcaac ttttgaccaa
    32521 tgagacctag cttaaggttt tcttcaatac tttggttttc aattaagcca aagttctgga
    32581 agaggtagcc caattcgtga cggaaaaaat cactcgattt ataattggcc aagtctttac
    32641 ctcggtaaaa aatcgtccca tcataaggtt ctaatttccc aatcatgttc atcaaggttg
    32701 tttttccgct accacttgaa ccaattaagg catagacttt tccagcctca aatgtcattg
    32761 aaagattcga aaataactct cgttctccaa aagatttact cacctgttta agttcaatca
    32821 tattaacctc ccttcaaaac aagaatggac atcttgtttt ctttctgcat ttggacatgt
    32881 aactgtaaaa gagatagacc agtaaagagt aacaagacta agaaagcaac tcctatctct
    32941 acctgaagaa atacactcgc aacaaatccc agtaaaaaca cacccagttg agcaaagaga
    33001 taagtgcgat ggatttctaa gaacctgaga cctgcaatgc gtttgataaa aatggcacgt
    33061 ctaaattctt caaagtagag cctattcata gtgttaaaca acaagattga agttgcaatc
    33121 ccaagcacag ctcctgctag cattacccaa cgttccctct ggatattatc caataatgtg
    33181 atgtagttgt ggtaacctgt ttgcatttct gagacccaat tttcaatgcc ttgtctctgg
    33241 ataagctcct gggcatcaga caattgatta aagagaacgt agttctgtac tgcgtctatc
    33301 caaaacaaaa tggactgtgg accagttgat tggggtgtta taacaatgat gattggatct
    33361 ttcaaaaact gctggtaaga aataggggtc gtattataca caaaacgatc ctgacctgat
    33421 tctaaataac ctaccgtagc agtcatttgc tgtcgttcat cttgactaga catgcgactg
    33481 gttaagtcgt cttcaaaaac agatttataa tgttcttcct ctgaacggag gtgttcaggc
    33541 agcaataaga caaactcccc tacattaagg tgattcatct tttgctcaat agttgtatct
    33601 acaggaatgt tttgacgctc caagtattgc ggtgtgacga taaggacatt gccattcggg
    33661 ttgtaatcgt gccattctgt agaggttatc aagtttttgg aggaagccat gccattttgc
    33721 aaagtacggt caattaactg gtgtctagat aagaaagcct tttgttctga aacagccaga
    33781 tccatcaatt gataccaagt tctgagtttc ctttgagctt gttcatcaaa accaggactc
    33841 gttccttcac ggctgattga tagggtaatc agttgccttt cctgactcca agcctcttgt
    33901 ccaatccgat gctgttgcca ggcttgggaa tactttaagc tactccctat tcccagcgtt
    33961 acaataataa tcgctaatag ttgaccgatt aaaatcaaac taatgattcc tctgacaggc
    34021 acttgccctt ttataatctg catcaggtgt atctttttta tcccaactgc gaagagttga
    34081 gcgaaaaaca gggagatcga caacaagatg agattataac tgagcaagcc tgctcctatg
    34141 gtcattaggg attgcgttgg aagcgacaga attttttgca taagaatggc gagcacgcca
    34201 gctaaactga aaccaacagc tatccctttc aaatcctcac caactggtct aaggaaaatg
    34261 gaccaccgtt tctctcctga aatgaggcga atgcccgacg accgtaattg gctaatttga
    34321 ctaattaaag tcagtgctac aaaggttaag atgaaaatca atagactgat taactgaaat
    34381 ccgttactaa agatagccat taacgtagat agtttagatg gaattgtcag gttcatgtta
    34441 gtcaagccaa gttgacttag ctcctccctt agcaagtgaa tatctaggtg tccatcgaat
    34501 acaaaatagt ttgtttcaac actactactt tgagcatctt ctagtttttt ttcctgaagc
    34561 ccatcaggca actttccgtc cccaaaggtc gtataagaaa aaactgtagt accttctgag
    34621 ttagaatctt ggtgttgaat cgcaataaag ctatctgttt cttctgctag tttttccaag
    34681 cgtgtagcaa catgctgaaa agttgtttca ggggaagaat cacgtacaac aacgttgggg
    34741 taataatggg agacatatgt atcagtccaa atggtaaata cccaaacaaa gaacaaacta
    34801 gcaagcaggt tggatatgag tataaataat tttttcatag tgcattcctt attctaaaac
    34861 agagggcaga aatacccctg ccctctataa actaacaag
//
LOCUS       DAYYQN010000014        34800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYYQN010000014 DAYYQN010000000
VERSION     DAYYQN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.014"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..188
                     /locus_tag="ACHBRQ_000630"
     CDS             <1..188
                     /locus_tag="ACHBRQ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFM9592344.1"
                     /translation="ATLFNSLNIDQVQLLPFHQFGENKYRLLNRKYEMDGVNALHPED
                     LIDYQKVFLNHHINCYF"
     gene            236..460
                     /locus_tag="ACHBRQ_000631"
                     /pseudo
     CDS             236..460
                     /locus_tag="ACHBRQ_000631"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            797..1798
                     /locus_tag="ACHBRQ_000632"
                     /pseudo
     CDS             797..1798
                     /locus_tag="ACHBRQ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1811..2902
                     /locus_tag="ACHBRQ_000633"
     CDS             1811..2902
                     /locus_tag="ACHBRQ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592345.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2892..4527
                     /locus_tag="ACHBRQ_000634"
                     /pseudo
     CDS             2892..4527
                     /locus_tag="ACHBRQ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4630..5322)
                     /locus_tag="ACHBRQ_000635"
     CDS             complement(4630..5322)
                     /locus_tag="ACHBRQ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9592346.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(5566..6903)
                     /locus_tag="ACHBRQ_000636"
     CDS             complement(5566..6903)
                     /locus_tag="ACHBRQ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFM9592347.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(6913..7179)
                     /locus_tag="ACHBRQ_000637"
     CDS             complement(6913..7179)
                     /locus_tag="ACHBRQ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFM9592348.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(7444..7830)
                     /gene="rplQ"
                     /locus_tag="ACHBRQ_000638"
     CDS             complement(7444..7830)
                     /gene="rplQ"
                     /locus_tag="ACHBRQ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9592349.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(7842..8777)
                     /locus_tag="ACHBRQ_000639"
     CDS             complement(7842..8777)
                     /locus_tag="ACHBRQ_000639"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9592350.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(8820..9203)
                     /gene="rpsK"
                     /locus_tag="ACHBRQ_000640"
     CDS             complement(8820..9203)
                     /gene="rpsK"
                     /locus_tag="ACHBRQ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9592351.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(9221..9586)
                     /gene="rpsM"
                     /locus_tag="ACHBRQ_000641"
     CDS             complement(9221..9586)
                     /gene="rpsM"
                     /locus_tag="ACHBRQ_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9592352.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(9604..9720)
                     /gene="rpmJ"
                     /locus_tag="ACHBRQ_000642"
     CDS             complement(9604..9720)
                     /gene="rpmJ"
                     /locus_tag="ACHBRQ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9592353.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(9745..9963)
                     /gene="infA"
                     /locus_tag="ACHBRQ_000643"
     CDS             complement(9745..9963)
                     /gene="infA"
                     /locus_tag="ACHBRQ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9592354.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(10080..10718)
                     /locus_tag="ACHBRQ_000644"
     CDS             complement(10080..10718)
                     /locus_tag="ACHBRQ_000644"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9592355.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKECLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(10869..12179)
                     /gene="secY"
                     /locus_tag="ACHBRQ_000645"
     CDS             complement(10869..12179)
                     /gene="secY"
                     /locus_tag="ACHBRQ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9592356.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRIE"
     gene            complement(12192..12632)
                     /gene="rplO"
                     /locus_tag="ACHBRQ_000646"
     CDS             complement(12192..12632)
                     /gene="rplO"
                     /locus_tag="ACHBRQ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9592357.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(12777..12959)
                     /gene="rpmD"
                     /locus_tag="ACHBRQ_000647"
     CDS             complement(12777..12959)
                     /gene="rpmD"
                     /locus_tag="ACHBRQ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9592358.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            complement(12973..13467)
                     /gene="rpsE"
                     /locus_tag="ACHBRQ_000648"
     CDS             complement(12973..13467)
                     /gene="rpsE"
                     /locus_tag="ACHBRQ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9592359.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(13485..13841)
                     /gene="rplR"
                     /locus_tag="ACHBRQ_000649"
     CDS             complement(13485..13841)
                     /gene="rplR"
                     /locus_tag="ACHBRQ_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9592360.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(13925..14461)
                     /gene="rplF"
                     /locus_tag="ACHBRQ_000650"
     CDS             complement(13925..14461)
                     /gene="rplF"
                     /locus_tag="ACHBRQ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9592361.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(14653..15051)
                     /gene="rpsH"
                     /locus_tag="ACHBRQ_000651"
     CDS             complement(14653..15051)
                     /gene="rpsH"
                     /locus_tag="ACHBRQ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9592362.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(15336..15605)
                     /gene="rpsN"
                     /locus_tag="ACHBRQ_000652"
     CDS             complement(15336..15605)
                     /gene="rpsN"
                     /locus_tag="ACHBRQ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9592363.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(15623..16165)
                     /gene="rplE"
                     /locus_tag="ACHBRQ_000653"
     CDS             complement(15623..16165)
                     /gene="rplE"
                     /locus_tag="ACHBRQ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9592364.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(16189..16494)
                     /gene="rplX"
                     /locus_tag="ACHBRQ_000654"
     CDS             complement(16189..16494)
                     /gene="rplX"
                     /locus_tag="ACHBRQ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9592365.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(16572..16940)
                     /gene="rplN"
                     /locus_tag="ACHBRQ_000655"
     CDS             complement(16572..16940)
                     /gene="rplN"
                     /locus_tag="ACHBRQ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9592366.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(16966..17226)
                     /gene="rpsQ"
                     /locus_tag="ACHBRQ_000656"
     CDS             complement(16966..17226)
                     /gene="rpsQ"
                     /locus_tag="ACHBRQ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9592367.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(17251..17457)
                     /gene="rpmC"
                     /locus_tag="ACHBRQ_000657"
     CDS             complement(17251..17457)
                     /gene="rpmC"
                     /locus_tag="ACHBRQ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9592368.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(17467..17880)
                     /gene="rplP"
                     /locus_tag="ACHBRQ_000658"
     CDS             complement(17467..17880)
                     /gene="rplP"
                     /locus_tag="ACHBRQ_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9592369.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(17884..18537)
                     /gene="rpsC"
                     /locus_tag="ACHBRQ_000659"
     CDS             complement(17884..18537)
                     /gene="rpsC"
                     /locus_tag="ACHBRQ_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9592370.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(18550..18894)
                     /gene="rplV"
                     /locus_tag="ACHBRQ_000660"
     CDS             complement(18550..18894)
                     /gene="rplV"
                     /locus_tag="ACHBRQ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9592371.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(18906..19187)
                     /gene="rpsS"
                     /locus_tag="ACHBRQ_000661"
     CDS             complement(18906..19187)
                     /gene="rpsS"
                     /locus_tag="ACHBRQ_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9592372.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(19291..20124)
                     /gene="rplB"
                     /locus_tag="ACHBRQ_000662"
     CDS             complement(19291..20124)
                     /gene="rplB"
                     /locus_tag="ACHBRQ_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9592373.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(20142..20438)
                     /locus_tag="ACHBRQ_000663"
     CDS             complement(20142..20438)
                     /locus_tag="ACHBRQ_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9592374.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(20438..21061)
                     /gene="rplD"
                     /locus_tag="ACHBRQ_000664"
     CDS             complement(20438..21061)
                     /gene="rplD"
                     /locus_tag="ACHBRQ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9592375.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(21086..21712)
                     /gene="rplC"
                     /locus_tag="ACHBRQ_000665"
     CDS             complement(21086..21712)
                     /gene="rplC"
                     /locus_tag="ACHBRQ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9592376.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(21929..22237)
                     /gene="rpsJ"
                     /locus_tag="ACHBRQ_000666"
     CDS             complement(21929..22237)
                     /gene="rpsJ"
                     /locus_tag="ACHBRQ_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9592377.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(22503..23120)
                     /locus_tag="ACHBRQ_000667"
     CDS             complement(22503..23120)
                     /locus_tag="ACHBRQ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9592378.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(23117..23707)
                     /gene="nrdG"
                     /locus_tag="ACHBRQ_000668"
     CDS             complement(23117..23707)
                     /gene="nrdG"
                     /locus_tag="ACHBRQ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9592379.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(23700..24206)
                     /locus_tag="ACHBRQ_000669"
     CDS             complement(23700..24206)
                     /locus_tag="ACHBRQ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592380.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            complement(24250..24390)
                     /locus_tag="ACHBRQ_000670"
     CDS             complement(24250..24390)
                     /locus_tag="ACHBRQ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592381.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(24402..26609)
                     /gene="nrdD"
                     /locus_tag="ACHBRQ_000671"
     CDS             complement(24402..26609)
                     /gene="nrdD"
                     /locus_tag="ACHBRQ_000671"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9592382.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(26729..28267)
                     /locus_tag="ACHBRQ_000672"
     CDS             complement(26729..28267)
                     /locus_tag="ACHBRQ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HFM9592383.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHNRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSLRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(28525..30057)
                     /gene="cls"
                     /locus_tag="ACHBRQ_000673"
     CDS             complement(28525..30057)
                     /gene="cls"
                     /locus_tag="ACHBRQ_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9592384.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(30203..30646)
                     /locus_tag="ACHBRQ_000674"
     CDS             complement(30203..30646)
                     /locus_tag="ACHBRQ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HFM9592385.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPAQQPAQQNTNTA
                     NAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKV
                     KQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(30730..31980)
                     /locus_tag="ACHBRQ_000675"
     CDS             complement(30730..31980)
                     /locus_tag="ACHBRQ_000675"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9592386.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISIFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(32591..32896)
                     /locus_tag="ACHBRQ_000676"
     CDS             complement(32591..32896)
                     /locus_tag="ACHBRQ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9592387.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(32912..33331)
                     /gene="ruvX"
                     /locus_tag="ACHBRQ_000677"
     CDS             complement(32912..33331)
                     /gene="ruvX"
                     /locus_tag="ACHBRQ_000677"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9592388.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(33335..33601)
                     /locus_tag="ACHBRQ_000678"
     CDS             complement(33335..33601)
                     /locus_tag="ACHBRQ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9592389.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(33688..34257)
                     /locus_tag="ACHBRQ_000679"
     CDS             complement(33688..34257)
                     /locus_tag="ACHBRQ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HFM9592390.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(34323..34721)
                     /gene="spx"
                     /locus_tag="ACHBRQ_000680"
     CDS             complement(34323..34721)
                     /gene="spx"
                     /locus_tag="ACHBRQ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFM9592391.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         9955 a         7682 c         6337 g        10826 t
ORIGIN      
        1 tcgctactct atttaactca ttaaatatcg accaagttca actactccct tttcatcaat
       61 ttggtgaaaa caaatatcgt ttattaaatc ggaaatatga aatggatgga gtcaacgcac
      121 ttcatcctga agatcttatt gattatcaaa aggtatttct gaaccaccat attaattgtt
      181 atttctagtt tatttccttg aaatgctcta gctatttgca gataacaagc atctatataa
      241 ggttatcaaa gaagacttcc ccatgactct aaatgaatat atcttacaat atcgcctgaa
      301 acaagctata gataagatgg ctgaatctcc caactcctct ttaagcgata tctctgacca
      361 agttggattt tcagactata aatattttgc caaagtattt aaaaagtatc tccatatttc
      421 cccaaagaaa ttgaaatcac tcggaagaat agtaaaatag gctattcttc tttttgtaga
      481 gaattttact ctaatatagt gaattgaaat aagatgtgaa catctctatc aggaaagtca
      541 aattaattta tagaaatatt ttagcagtca aagtgtactg ttatagattc aatacactat
      601 agaatagaat tgtaggtttt aaaaacgctt acattgtttt aaaataaact taaataacac
      661 aacggaggta tccatcatga aaaagaaatg gatgtattat gctgcttgtt cttctaatga
      721 atctgccgat gacagttcat ctgataaagg agacggtggt tcgctagtcg tttattgacc
      781 aaactcagaa ggcttaattg gagcaactat tcctgccttt gaagaaaaat atggtatcaa
      841 aatagaactg attcaagctg gtactggaga acttttcaaa aaactagagt cagaaaaaga
      901 agttcctgta gctgatgtta tctttggtgg ttcttataca caatatgcta cccacggaga
      961 actctttgaa aactatattt caaaagaaaa tgataatgtt atcaaagaat atcaaaacac
     1021 aactggcttc tctactcctt atacactaga tggtagtgtt ttaatcgtcc accctgattt
     1081 aactaaaggc atgaacatcg aaggatatag cgaccttctc aaacctgaac taaaaggaaa
     1141 aatcgcaact gctgacccag caaactcttc tagcgccttt gctcaattaa caaatatgct
     1201 acaagctcaa ggtggttaca aagatgataa ggcttggtct tatgtaaaag atcttttcac
     1261 acttattgat ggtatagtaa aatgaaataa gaacaggaca aattaatcag gacagtcaaa
     1321 tcgatttcta acaatgtttt agaagtagat gtgtactatt ctagtttcaa tatactataa
     1381 aatcggttca agttcatctg gtgtctataa agcagtcgct gatggagaaa tgactgttgg
     1441 tctctcttat gaagatccag cagttaaact cttaaatgac ggagctaaca ttaaggtagt
     1501 ctatccaaaa gaaggaaccg tcttcctacc ttctagtgct gctatcgtta aaaaagctaa
     1561 aaatatggaa aatgccaaga aatttatcga ttttattatc tctcaagaag tacaagatac
     1621 acttggtaca accactacta accgtcctgt tcgtaaaaat gctaaaacaa gcgaaaacat
     1681 gaaaccaatt gacaaaatca aaacactcac ttaagattat gattatgtca tcaagaataa
     1741 atcagatatc gttaagaaat acaacgaagt ctttacagat atccaatcta aacagtaaaa
     1801 gaggttcact atgagtgaga tcgaaattat taacgccaaa aaaatctacc acgatgtccc
     1861 tgttattgag aatttgaaca ttacaattcc aaaaggaagt ctctttaccc ttcttggagc
     1921 ttcaggatgt gggaaaacga cccttcttcg tatgattgca ggtttcaaca gtatcaaaga
     1981 tggagaattt tacttcgatg atacaaaaat caataatatg gaacccagca aacgcaatat
     2041 cgggctggtt ttccaaaact acgctatttt cccacatttg actgtccgag acaacgttgc
     2101 ttttggtctt atgcaaaaga aggttccaaa agaagaattg attcaacaga ccaacaagta
     2161 tcttgaactc atgcaaattg ctcaatatgc ggatcgaaag cccgataaac tcagtggtgg
     2221 acaacaacaa cgtgtcacct tggcatgcgc cttagcggtt aatccaagtg ttctcctcat
     2281 ggacgagcca cttagtaatc tggaggccaa acttcgcttg gatatgcgtc aaaccatccg
     2341 agaaatccaa cacgaagtgg gaattacaac tgtttatgta acccacgacc aagaagaagc
     2401 catggctatt tcagaccaaa ttgctgttat gaaagatggg gtgatccaac aaatcggccg
     2461 accaaaagaa ctctatcata aaccagctaa tgagtttgtg gcaaccttta tcggacgcac
     2521 aaatattatc cctgccaatc ttgaaaaacg gagcgacggc gcttatatcg tcttttcaga
     2581 tggctatgcc cttcgaatgc tagctcttga tcaggttgag gagcaagcta ttcatgtaag
     2641 cattcgtccc gaagagttta tcaaagatga atctggagat atcgaaggaa ctattagcga
     2701 tagcgtctat cttggactaa atacggagta tttcattgag acaggttttg cctcaaaaat
     2761 tcaagttagt gaagaatcaa cttttgaaga agatctacaa aaaggcaatc gtattcgtct
     2821 acgaatcaat acgcaaaaat taaacatctt ttctgcagat ggttcccaaa acctgataaa
     2881 aggagtcaac catggaatgt aaaaaactaa atatttggac agcctcctct ttcttcctct
     2941 ttcttaccta tcttgtcttt ctcgtttatc ctatcgttac cgtgctcaag caagcactta
     3001 tacatgaagg acaattctca ctagctaatt ttgtcacttt ctttagtaaa gcctactact
     3061 ctgagacact agtcaacagt ttcagggttt ccattaccgc tactgtcact tccttagttg
     3121 taggaaccct attagcttat ctcttctcta tgtatgactt caaggggaag aaatttctac
     3181 aaatattgat tatcattgct tccatgtcag ctcctttcgt aggagcctac tcctggattc
     3241 tcttgctggg acgaaatgag gtcatcacta aatttttgac aaatgccctt tatcttccag
     3301 ctatcgatat ttatggattc aaaggaattg tacttgtctt tacactgcaa ctattcccac
     3361 tggtatttct atatgttgct ggaacaatga acagtattga caattctcta cttgaagcag
     3421 ctgaaagcat ggggtccttc ggatttaaac ctatcgtaac ggttgtttta cctctcctag
     3481 ttccaacctt actagcagct ccttgcttgt atttatgaga gcattctcag actttggaac
     3541 gcctatgttg attggcgaag ggtatcggac tttccctgtc ctgatttata cccaatttat
     3601 tagcgaggtt ggaggaaatt ctgcttttgc aattatggcg attatcattg ccttggcaat
     3661 tttccttatc caaaaacaca ttgcaaaccg ctacagtttc agcatgaatc tgctccatcc
     3721 aattgagcct aaaaaaacta caaaaggaaa aatggctgcc atttatgcaa cagtctacgg
     3781 aattatcttt atctctgttt tacctcaaat ctacttaatt tatacctctt tcctaaaaac
     3841 atcaggtatg gtatttgtta aaggttattc tccaaacagt tacaagctag ctttcaatcg
     3901 tatgggatct gctattttca ataccattcg tatccctttg attgccttag ttctagttgt
     3961 tctatttacg acatttatct cctacctagc cgttagaaaa cggaatttgt ttacaaactt
     4021 aattgacagc ctcagtatgg taccttatat tgtaccagga accgttctag ggattgcctt
     4081 catttctctg gtctatttgg aagtggattt cttatgatta cagggactgc tttcatcttg
     4141 attatgtctc tatctgccag aaaattacct tatactattc gctcatctgt tgctagctta
     4201 caacaaatag caccaagtat tgaagaagct gctgaaagct taggaagtag tcgtctcaat
     4261 atctttgcta agattacaac tccaatgatg ctatcggata ttatttctgg agccatccta
     4321 tcttgggtca cactgatttc agaactctct acttctatcc tcctctacaa tgtcaaaaca
     4381 agaacaatga ctgtagctat ttatacagag gttctcagag gaaattacgg tgtagctgca
     4441 gccttgtcaa ctatcctgac tgttctaaca gtaggttcct tgctcttgtt tatgaaaatc
     4501 tctaaaagca atagcattac actttagttt tccacatcaa aaacagccga aaggagtacc
     4561 ctcggctgtt ttttcttatc ttgatattct tatcaaatcc ctagctcatg gcaagcaagt
     4621 ctagactgat taaatagaac cttcttccat cttctcacgt cctagctctc ggtaactacg
     4681 gtcaccgaca acttcaaccc taaactgacc gtcctcatat tcaagaatcg tcacgctacc
     4741 attatctaga ccatgcggat gcatgccatt aatcagataa acaatggttc caatagtcat
     4801 tccatggctg acaacaaggg cgttacctcc accttgatct tccatttctt ttgcaattat
     4861 ttcaaagcct tccttgattc ggccactgag tttttcccaa ccttcagccc aaccagctgt
     4921 atcgacctct accaagccct cagccagttc agcataagac aattggtgaa cgtggtccac
     4981 attaaagata cgaggaataa tgcccatgaa aagatcgcca tcataggctc catcaaaact
     5041 accgaaacac cattctctga tacgcttgtc catgcgataa gggattttcc cctgcaagcc
     5101 aagttcttca agtataattc ccatggtctg aatggtacga ccagaatcac tcgaataagc
     5161 gcgctcaaac tgtagatcag attctcgcaa accgattcct aactcttgaa tccctcgttc
     5221 accttcagca gttaagggag tatcgctcca accttgcgcg cgaccaatcg tgttaaacat
     5281 ggtcttgcca tgacgtacca aatacaatcg tacttttacc attttccgtc ctccgtttgc
     5341 ttctattata tcatgaatga taaaataaaa gccacccata caggcgactt ttgaaggaga
     5401 ttattatgaa aaagtttagg agttcattaa ataaagatat tagatgaaaa tcaaattcaa
     5461 actaattcag tgttatctgt ttcaaataat attaggaggt ctttatttaa tgattatagt
     5521 atacacatct aatcttaaat agaacttaaa atttctccta ttttcttaat ttttaaaact
     5581 atgaattggt gctgggattt gtccaccgcg agagatgaag tcgacagacg aagccccatt
     5641 aaccttcata acgggtgcag ttcctaatag accaccaaac tcaatcatat cgccttctct
     5701 tcctttggga atgatacgaa cagctgttgt tttcatgttg ataacaccga ttgctgcttc
     5761 atccgcaatc atagccgcaa tagtttcagc aggcgtatct tctgggatgg caatcatatc
     5821 caatccaaca gaacagatag ccgtcatagc ttctagtttt tctaaattaa gagagccatt
     5881 ttgcactgca gcaatcattc cttcatcctc agaaacaggg ataaaggcac cagatagacc
     5941 accgacctgg ttacaggcca tcactccacc ctttttaact tggtcgttca agagggccaa
     6001 ggcagctgtc gttccatgcg tgccaactgt ttctagcccc atttcctcaa ggacacgtgc
     6061 cacagagtct ccaaccgcag gggttggtgc caaactcaag tccacaatac caaactccac
     6121 acccagtctc tcactggcca tttgaccaac caattgaccg atacgagtga ttttaaaggc
     6181 agttttctta actgtttcgg ctactacatc aaagctctgt ccacgaactt tttccaaagc
     6241 acgtttcaca acaccaggac cagaaactcc gacattgatg ataacatctg cttccccaac
     6301 accatgaaag gcacccgcca taaatggatt gtcctcaaca gcattagcga atacaaccaa
     6361 cttggccact cccatatctg aaagatttgc tgtttccttg ataattcgtc ccatatctgc
     6421 cacagccgtc atattaatac cagacttggt tgagccgata ttgactgacg agcagacctt
     6481 atccgtctca gccaaagcgc gaggaatgga attgatgaga atctcatctc ccttttgata
     6541 acctttttgt actaaggcag aaaaaccacc aataaagtcc acaccaatct ctttcgcagc
     6601 cttatcaagc gcttttgcca gaaccacgta gtccgtcgca tctgtcgctg ccccaatcag
     6661 agaaataggt gtcaccgata cacgcttatt aacgatagga attcccaact cagccgcaat
     6721 ttcatcacca acagctacta aattagccgc ctttgtcgta attttttgat agattttctc
     6781 cgcagcacga ttgatatttg gatcgataca gtccaaaaga gaaatcccca tggtaatggt
     6841 tctaatatcg aagttttgct cctcaatcat ggcgatggtt tcagtaactt gtctaatatc
     6901 catgatgacc tcctagatat tatacatagc ttcgaaaatc gctgcactct gaatattgat
     6961 ttttacattc aaagtttgcc caaaagcttc aaattcatta cgaagatagg taaaatcttg
     7021 cttttcatca ctagatacaa cagccatcat cgtaaaatat tcatccaata cagtttgaga
     7081 gatatcgtca atattcaatc ctaattctgc aattttacca gaaacacctg caacaattcc
     7141 agatttatct ttaccaacaa cagttataat cgctttcata agtaaactcc aattcttctt
     7201 aaaataagaa accttaacat taaacaggat tcttttcaaa tagtatagca taattctaac
     7261 gaaaatactc aaaaacgcct gcttacgaag ttgttcttaa gaaaaaaaca atttcgtaaa
     7321 caagcgtcta ctatcccaac ttatgatgag tgttcccgct aggaccgaaa tcctagcggt
     7381 agaccagagc tagactaaga gcacaagact ccatcatcat aacactcaac aaaattgatg
     7441 attttatact aattcgatga tcgccattgg cgctgcatca ccacgacgtg gttcagtttt
     7501 aaggatacga gtgtatccac cgttacgttc agcataacga ggtgcgattt ctgagaacaa
     7561 tttttgaagt gctgtagtag aagtgtactt atcagttgct tcatcatagt tttcagatgc
     7621 gatttcatta cgtacgaaag cagctgcttg acgacgtgca tgcaaatcac cacgtttacc
     7681 tagagtaatc attttttcaa cagttttacg gatttcttta gcacgagctt cagttgtcac
     7741 gattgattcg ttgatcaaaa ggtcagttgt caaatcgcga agcattgctt tacgttgtga
     7801 gctagtgcgt cctagtttac ggtaagccat gtattcctcc tttatttatc ttttaatcca
     7861 agacccaaat caatgagttt gagtttcact tcttccaaac tcttgcgtcc aagatttcgt
     7921 actttcatca tctctgcttc agatttttct gtcaaatcat gcacagtatt gataccggca
     7981 cgttttaaac agttgtatga acgcacagac aagtccagtt cctcaatcgt acgatctaaa
     8041 atacggtcgt cagattcagt atcagcttct ttcatcactt cagttgactt agcaatctca
     8101 gtaagatttg taaacaaatc aagatgttct gtcaaaatac gtgctgaaag ccctaaagca
     8161 tcttctggaa taattgttcc atttgtcaag atttcaaggg ttaatttgtc gaaaccatca
     8221 ttgctaccta cacgagcagg ttccacttga tagttgactt ttgtaactgg tgtataaata
     8281 gaatctacag caagtgttcc aactggtgca ttatcctttt tattttcatc agcaggtaca
     8341 tatccacgac cactgttaac agtcatagtc gcttttagag aagaaccttc accaattgta
     8401 aagagataat gatctggatt tacaatttca atatcgctat ctgtcaaaat gtcaccagct
     8461 gttacttcag caggaccttc aacatccagt tcgatgattt tttcgtcttc aacgtacgat
     8521 ttcactgcaa ttcctttaat gttcagaatg atttgcataa cgtcttcacg aacacctgga
     8581 actgtgtcaa actcatgtaa cacaccatca atgttgatag atgtcacagc tgctcctggt
     8641 agagaagcta gaagtacacg acgaagagag ttaccaagag ttgtaccgta gccacgttca
     8701 agtggttcga ttacaaactt gccataatct ttattttcat caatttttgt tatatttggt
     8761 ttttcaaact cgatcattta gttactccct cttaaacgaa aagcagtgta atgcgatgat
     8821 tatacacggc gacgttttgg aggacgagca ccattgtgtg gcactggagt cacatcacga
     8881 attgctgtta cttcaagacc agcggcagca agcgcacgaa tagctgactc acgaccagaa
     8941 cctggacctt ttacagtaac ttcaactgat ttaagaccgt gttcttgtgc agatttagca
     9001 gcagcttcag aagccatttg agcagcgaat ggtgtagatt tacgagaacc tttgaaacca
     9061 agagcaccag ctgatgacca agcaattgca ttaccatgca catcagtaat cataacaata
     9121 gtgttattaa atgtagcgtg aatatgagca ataccagatt cgatattctt tttcacacga
     9181 cgtttacgtg ttggtttagc caagactttt acctcctata ttattttttc ttaccagcaa
     9241 tcgcaacagc tttaccttta cgagtgcggg cgttgttttt agtgttttgt ccacggacag
     9301 gaagtccacg acggtgacgg ataccacggt atgaaccgat ttccatcaaa cgtttgatgt
     9361 tcaagtttac ttcacgacga aggtcacctt caactttgat tgcatccact tcacgacgga
     9421 tagcatcttc ttgatctgat gtaagatcac gtacacgaac atcttctgag attccagcag
     9481 cagccaaaat tttcttagat gttgcaagtc cgataccata aacataagtc aatgagatta
     9541 ctacgcgttt gtcatttgga atatcaactc cagcaatacg agccatgttt tctcctttct
     9601 atcttatcct tgacgttgtt tgtgttttgg atttgctggg caaattacca taacacgacc
     9661 attacgacga ataactttac agtattcgca aattggtttg accgatggtc ttactttcat
     9721 ttcttatccc tccaagtttt tcgattattt aaagcggtaa gtgatacgtc cacgtgtcaa
     9781 gtcatatgga ctcatttcga cagtaacacg atctcccgct aaaatacgaa tatagttttt
     9841 acgaatttta ccagaaactg ttgctaaaat ctgatgtcca ttttcaagtt caaccgtaaa
     9901 cattgcattc ggcattgtat caactacttt gccttcaact tcaatcacat cgtcttttgc
     9961 cacgcaaaag cacctccata aatttcgatt cgatgcctct agacacagag acaacaatta
    10021 taagtcagac tatctcagta taacatttgt agcggatttt tgcaagtgcg aaaaacgctt
    10081 tatttcaaat ttgtcaatac tttttcaata tctgagaaga catcattgat atcttgatta
    10141 ccttcgatgt catgaaccaa acctttggca cggtagtgag caatgattgg ttctccttga
    10201 gcaatattaa catccaaacg acgttttact gtctcaggct tatcatcttc acgttggtag
    10261 taatcttctt ctttatagtc aactggtggg ttaaagacct tgtggaaagt ttctccagtt
    10321 acgcggtgga tgatacggcc actcaaacgt tccaagaggc tgtcagggtt cacttcaata
    10381 ttgataatac cttctagttc aatgccaagt tcagccaatg ttttgtccaa ggcatgagct
    10441 tgttcaattg tacgtgggta accatccaat aagaatcctg tttctttaat atcatcttgt
    10501 gaaaggcatt cttttacgat tccatttgta acttcgtcag gaaccaattc acccttgtca
    10561 atatatgact tagcaagaac acccatttca gtttgatttg ccattgcagc gcggaacata
    10621 tcacctgttg agatatgtgc aacatggaat tgttctacga tttttgctgc ttgagttccc
    10681 ttacctgcac caggtaagcc cataatcaaa agattcatga tttgatctcc ttattttatt
    10741 tttaaataga agcaaaaagt cttttcaccc ctatttaaaa acaaaataga agaggggaga
    10801 tccaactctt acaaatgtca gagtttaaac ctccactccc tcagtattta ctgattcagt
    10861 aaatactttt attctattct gtccatgaaa ccaacatact tacgtttcaa taggtaacct
    10921 tccaattgct tgattccttc gatacctgta gagataatga tcaagagact tgttccacca
    10981 aaggcaacaa catcagaaag accaaataca tctttagctg caatcggtaa aatggaaatc
    11041 acaccaagga agagggaacc aacagttgca agacgacgaa gaagtttaga catatattct
    11101 tctgtacctt taccaggacg aactccatgg atataggcac cactcttttg taggctctct
    11161 gctgcttttt caggattaat ctgtacaaac gtatagaaga atgtaaagag aataatcaac
    11221 aaagcataca tggcaatacc agttggagaa gtagttgcca acatctcttg tgctaccctt
    11281 acccaagccc aatcatgacc tgtggcactc aaaaactgaa gaatagccgc aggcgctgca
    11341 gtaatcgaac tggcaaagat aacagggata actccagcag ggtttacctt taacggaagg
    11401 taagagctag atggagcacc ttgtgcaacc ttagtatatt ggattggaat tttgtattct
    11461 gcttgttgaa cataagttgt aaagtaaata atcaacaata cagtaataat caaaatgatt
    11521 acgaaaatga tagatgaagt gatacggcta cttgggacgt tcacaaagta gtccacatag
    11581 atgccctgaa tcatctctgg aattgaggaa acaatcccgg caaagataat catggaaaca
    11641 ccgtttccgt atcccttatc tgtaatttgc tcacccaacc aagtgacaat catactacca
    11701 gctgttaaga tgataccaat cgtcagaaaa acttgtggag ttaaagcagt tttaatcaat
    11761 tgagctccag ccaaggtatt aaaaccagct gtaatcccga tagattgcac aaaagcgaga
    11821 actagagcaa tataacgagt agcttgattc aattttcttc gacctacttc cccttgttta
    11881 ccccactcta caaacttggg taaaatatcc atttgcaaga gttggacaac aatagaagcg
    11941 gtgatatagg gactaactcc tagggcaaaa atcgaaaagt tttttagggc attccccgac
    12001 accaagctca acatgtttaa gaaggataat ccacttaaag cattcaagct attggcattc
    12061 acaccaggaa ctgtaatgct agttccgata cgaaagacca aaacgataaa aattgtaaat
    12121 aaaatttttg atcgaacctg cttgacttta agagcttctc ttaataattt aaaaaacata
    12181 ggtcacctct cttagatgac ttctactgaa ccacctttag cagtgatagc ttcttcagct
    12241 gatttagaga atttagctgc tttcacagtc aatttcttag tcaactcacc gttaccaaga
    12301 attttaatac ctgacttttc agctttaaca attcctgctt cgataagaac aactggagtt
    12361 acttcagcac catcttcaaa gacgttcaat tggtcaaggt tcacaattgc gtattcttta
    12421 gcgttgatgt tagtgaatcc acgttttgga agacgacgga acaatggagt ttgtccacct
    12481 tcaaaaccaa ggcgaactcc gccaccgcta cgagcttttt gacctttttg accacgacca
    12541 gatgttttac cgttacctga tgaagtacca cgaccaacgc ggttacgtac tttacgagaa
    12601 ccttctgcag gtttcaattc atgaagtttc atttttattt tctcctcttt tgtaaaatgc
    12661 tagcgccgat aagggagaaa aggttgtctc ccccatcaac tcgcctatac gatgtcatca
    12721 tagatgacta tatctagttt taggggatgg tacagtgcac atcccctaaa acttcattag
    12781 tttacttctt caactgttac taagtgagat actgctgtaa tcataccacg gatagcagcg
    12841 ttatcttctt taataacaga gctgttcaat ttgccaagtc caagtgctac aacagtttta
    12901 cgttgtgatg gaatgcgtcc gattggagac ttagtcaaag taattttaat ttgagccatt
    12961 ttatcccctt tcttatgcca aatcagaaac tgaaatacca cgaagggcag caatttcttc
    13021 agcgcgtttc aattgtttca aaccttcaac agttgcacga acaatgttga ttggagtgtt
    13081 agaaccaagt gatttagatg taatatctgc cacacctgcc aattccacaa cggcacgaac
    13141 tgcaccacca gcggcaactc cagaaccttc tacagcaggt ttcaacaata ctttagctcc
    13201 accgaattct gaaagaactt cgtgtgggat tgttgttcca accataggaa cttcgatcaa
    13261 gtttttctta gcatcatcta ctgctttacg gattgcttct ggaacttctt gagctttacc
    13321 agtaccaaat cctacgcgac cattgtggtc accaacaaca acaagagctg cgaaacgaag
    13381 acgacgtcca cctttaacaa cttttgtaac acggttgaca gcaactacgc gttcttctaa
    13441 ttcaactgca ttgtctttaa atgccatttt ctagtgtcct cctattagaa tttcaatccg
    13501 ttttcacgag ctgcatcagc caaagctttc acacgtccgt gatatagata tccaccgcgg
    13561 tcgaacacca cttctgaaat acctttagcg tttgcacgtt ctgcaacgag tttaccgaca
    13621 gcaacggctt gttcagtttt agttcctttt gaaacttctt tatcaagagt tgaagcactt
    13681 gcgagcgtta cacccgctac gtcatcaatc acttgagcgt agatgcctgt attagaacgg
    13741 aatacgttca aacgtgggcg atcagcagtt ccagagagtt ttccgcgaac gcgacggtgg
    13801 cgtttttggc ggagtttgtt tttatctggt tttgaaatca cagttttcac ctctttagtt
    13861 ttaaatcgtg tgctatgcac aaagttggaa aataggttgg tggttgatga tcaaccactc
    13921 aacattattt acctgtttta ccttctttac ggcgaacgaa ttcaccaacg taacggatac
    13981 ctttaccttt atatggttct ggtgaacgaa ggctacgtac gtaagcagct gtttgaccaa
    14041 ctacttcttt tgaaattccg ctaacaacga ttgttgttgg gtttggaagt tcaaaagtaa
    14101 ttccttctgg agcttcaact tcgtctggat gagatttacc aacagccaaa acaagtttag
    14161 atccttgaag ctgtgcacgg taaccaaccc cacgcatttc aagttctttc ttgaatcctt
    14221 ctgatacacc aacaaccatg ttgttcaaaa gggcacgagt agttccgtgg atagttttca
    14281 tttcttttga atcgtttgga cggtgaagag ttatttcagt accttccaca cggatttcaa
    14341 tatcttttga gaactcacga gtaagttctc ctttaggtcc ttttacagtt acaacgttgt
    14401 cattgttagc gagttcaaca ccagcaggca acacgataac tttattacca atacgtgaca
    14461 tgtttatttt ctcctgttaa attgtcaggc cagaacggcc agttttcacg gggttcagat
    14521 acttatttag ttcaagagct gaactgaaca cgctctaaga actgtgcaaa atagataagt
    14581 tggcttgttt gtaaacaaac ttctccaact tcctaatttt gctttgttct ttacgagctt
    14641 tgtatcttga ttttaccaaa cgtaagcgat aacctcacca ccaacattct tttggcgtgc
    14701 ttctttatca gtaagcaaac cttcagaagt tgaaaggatg gcaattccaa gtccgttaag
    14761 aacttttgga aggtcttcac gttttttgta gacacgaagt cctggtttag aaacacgttt
    14821 caagttagtg ataacttttt caccatttgg tccgtattta agaaatacac ggatgacgcc
    14881 ttgtttgtca tcttcgatga tttcaacgtt ttttacaaaa ccttcgcgtt taaggatttc
    14941 agcaatccct tttttgatgt ttgatgcagg tacttcaagt acttcgtgtt tagcttggtt
    15001 agcattacga atacgagtta ggaagtctgc gattgggtca gtcataacca ttttgttttt
    15061 ctcctcttac tagtagtttg caagttgcac ttgctagtta atacatgata caaagagcgt
    15121 aacagcacga gcaaaaatag acaatttaat gcagaagcaa tgctccaaag caaattggtc
    15181 ttttttgcca aagctgtagc tcgggttcaa attggcaagc ccaattgaac ccgggctaaa
    15241 ctctgtgtga aaaagataaa ctttcctaga aactttagtt tcttcgtcaa gtttcctatt
    15301 tttacttgga gttttgacac ccttgatatc ttaaattacc aagatgcttt tgttacacca
    15361 ggaatttgac ctttatgcgc aagttcgcga aaagcgatac gactcagacc aaatttgcgg
    15421 taaactgaat gtgggcgccc cgtaactcta caacgattat gtaaacgagt cggtgaggcg
    15481 ttgcgaggta atttagataa accttcgtag tcccctgccg cctttaatgc agcacgtttt
    15541 tcagcataac ggtcaacaat tttttggcgt ttagcctctc tagctaccat tgatttttta
    15601 gccattagat ttacctccta tattattttg caaaaggcat tccaaggcct gtaagcaatg
    15661 cacgtgactc ttcgtcagtg ttagcagttg ttacgataac gatgtcaaga ccacgagttt
    15721 tgtcaacgtc atcgaagttg atttctggga agattaattg ttctttcaca ccaagtgtgt
    15781 agttcccgcg tccatcaaat gattttgttg gaacaccgtg aaagtcacgt acacgtggaa
    15841 gtgaaactga taccaattta tccaagaatt cgtacatacg ttcaccacga agggtaactt
    15901 ttgcaccgat cgcaacacct tcacgaagac ggaagccggc gattgatttt ttagctttag
    15961 tgataagtgg tttttgacct gagataagtg ccaattcttc agcagctttt tcaaggcttt
    16021 tagcgtttga tacagcttca ccaacaccca tgttcaaaac aatcttatct actttaggca
    16081 cagccatcac tgatgagtag ttgaattgtt ctgtcaaagc aggaactact tcattaagat
    16141 atttttcttt taaacgattt gccattatac ttctcctttc cttcgtgatt aatcaagcac
    16201 ttcgcctgat tttttgttgt agcgaacttt tttaccgtct acaaatttgt aaccaacacg
    16261 accagctaca ccatttttgt ccaaaacttg aacgtttgat acgtggatag ctgcttcttt
    16321 ctcgatgata ccaccttgag gaagctcgtt agttggacgt tggtgtttct taacaatgtt
    16381 aacaccttca acgataactt tgtttacttt tggaagggca gtaaggacaa cagcttctgt
    16441 tcccttatct ttaccagcga ttacgcgaac tttgtcgcct ttttttacaa acattaggtt
    16501 tctccttgat ttttcttacg cccataaggg caccctagct tgaagctagg ggactagttt
    16561 gttcctaaaa attaaagtac ttctggagca agtgacacga tcttcatgaa gccaccttca
    16621 cgcaattcac gtgcaactgg gccaaagata cgtgttccgc gaggagtttt gtcttcacgg
    16681 atgataactg ctgcgttttc gtcaaatttg atgtatgaac catcagcacg acgagcacct
    16741 gatttagtac gaacgataac tgctttaaca acgtcacctt ttttaaccgc accaccagga
    16801 gtagcttgtt ttacagatgc cacgataaca tcaccgatgt ttgcaaattt acgtcctgaa
    16861 ccaccaagaa ctttgatagt caagatttcg cgagcaccgc tgttgtctgc gactttcaaa
    16921 cgagtttctg tttgaatcat ttcagttttc tcctttcagg tttgattaga tgatgaccgc
    16981 ttcttcaaca acttctacaa gacggaaacg ttttgtagct gaaagcgggc gagtttccat
    17041 gatacgtacg atatcgcctt ctttggcaac attgttttca tcatgagctt tgtatttttt
    17101 agagtagtta atacgtttac catagactgg gtggttacgt tttgtttcaa ctacaactgt
    17161 gattgtcttg tccattttgt cagatacaac acgtccaaca agaactttac gattattgcg
    17221 ttccattgaa atttctcctt ccctagtcta ttatttcgct tcagattgaa ctgttttgat
    17281 acgagcgatt tgttttttaa cttctttcaa gcgagctgtt tgttccaatt gaccagtagc
    17341 agcttggaaa cgaagttcaa acaattcttt tttcaattcg ttttcgcgct tcgcgagttc
    17401 ttcttgagaa agaccacgaa gttctttaac aaattctttt acttcattaa gtttcatgcc
    17461 ttctccttat tctgcttcac gttttacgaa tttacattta actggcaatt tgtggctagc
    17521 aagtcgaagc gcttcacgtg caatctcttc agatacacca gcgatttcga acatcacttt
    17581 accacgttta actggtgcta cccaaccttc aggtgcccct ttaccagatc ccatacgcac
    17641 accgatagct ttagcagtgt atgatttgtg tgggaagatt ttaatccaaa ctttaccacc
    17701 acgtttcatg taacgagtca tggcgatacg agcagcttcg atttggcggt tagtgatcca
    17761 gtggctagtt gtagcttgaa gaccgtattc accgaatgct acttcttttc caccttttgc
    17821 ttcaccgcgc atttttccac ggaattcacg acggtgttta acacgtttag gtactaacat
    17881 tggttattta cctcctttag tgtttttacg agctggaaga acttcaccac ggtagatcca
    17941 tactttaaca ccaagtttac catatgtagt atctgcttct tcccaagcgt aatcgatatc
    18001 tgcacgaagt gtgtgaagcg gaacagttcc ttcagagtat ccttcagcac gggcgatatc
    18061 tgcaccgttc aaacgacctg atacttgagt tttgattcct ttagctccag cacgcattgc
    18121 acgttggatt gcttgttttt gtgcacgacg gaaagcaaca cgttgctcca attgacgagc
    18181 aattccttca cctacaaggt gagcatccaa atcaggttgt ttgatttcga tgatgttgat
    18241 gtgtacttgt tttccagtca atttgttaag ttttgcacgg agtgcatcaa cgttagcacc
    18301 acctttaccg ataaccatac ctggtttagc agtgtgaagt gaaacgttaa ctttgtttac
    18361 tgcgcgttcg atttcaatag ttgaaactgc tgcgtcagca agttcttttt gaacgaattt
    18421 acggattgca agatcttcat gaaggtaatc cgcgtattct ttttcagcat accatttggc
    18481 atcccaatca cggatgatgc cgacacgcat accaattgga tgtacttttt gacccacgat
    18541 tttacctcct tatttttctg caacagctac agtgatgtga gctgtacgtt tgttgattgg
    18601 tgaagctgaa cctttcgcac gtggacggaa acgtttcata gttggtcctt cgtttgcgaa
    18661 tgcttcagat actaccaagt tagctttatc caaaccaaag ttgttttcag cgttagctac
    18721 agctgagttc aaaactttca agatgatttc agcagctttg tttggagtga atgtcaagat
    18781 tgcgattgca tcggctacgc ttttaccacg gatgttatca agaacaagac gtgatttacg
    18841 aggtgaaaca cgtactgtac gagccattgc tttagctgaa gtaatttctg ccatttatgt
    18901 tctccttatt ttctacgtgt tttcttgtcg tctgcagcgt gacctttgta agtacgagtt
    18961 ggtgcaaatt caccaagttt gtggcctacc atgtcttctt ggatgtaaac aggtacgtgt
    19021 ttacgtccgt cataaactgc aatagtgtaa ccaatgaaac ttgggaagat cgttgatcga
    19081 cgtgaccaag ttttaataac ttttttcttt tcgtcgttag cttgagcttc aacttttttc
    19141 atcaaatgct catcgacgaa aggtcctttt ttaagactgc gtcccatttt tatattttct
    19201 cctttaaatg ttgtaccaca gcggcttgca ctcctatgga gcgctaccga gctggcggat
    19261 ttactagttg cttaagcgac tagtttaata ttatttctcg ttgcgacgac gaacgataag
    19321 tttgtcagat ttcgctttct tgttacgagt tttaagacca agagcaggtt tgccccatgg
    19381 agtagatggt gctttacgac caactggtgc cttaccttca ccaccaccgt gtgggtgatc
    19441 gttagggttc attacagaac cacgaactgt tgggcggata cctttccaac ggctacgtcc
    19501 tgctttacca aggtttacaa gtccatgttg ttcgtttccg acaacaccaa ctgtagcacg
    19561 gcaagttcca agaatcatac gaacttcacc tgattgaaga cgaacaagaa cgtatttacc
    19621 ttcagaaccc aatacttgag cagatgcacc agcagcacgt accaattcac caccacgacc
    19681 tggtttcaac tcgatgttgt gaatcaaagt accaactggg atgttagcaa gtggaagagc
    19741 gtttccgact ttgatatctg cttctggacc tgaaacgata cgttgaccta cttcaagacc
    19801 ttttggagcg atgatgtatg ctttcacacc gtcagtgtag tgtacaagag cgatgtttgc
    19861 agaacggttt ggatcgtact cgattgtttt aacaactgct tcaacgttgt ctttattacg
    19921 tttgaagtca accaaacggt agaaacgttt gtgtccacca ccttggtgac gaactgtgat
    19981 acgaccgttg ttgttacgac cagccttgct cttcaatgca acaagcaatg atttttcagg
    20041 agtgcttgtt gtgatttcag cgaaatccaa agaagtcata ttacggcgac cgtttgttgt
    20101 tggtttataa acacgaattc ccacgatatt tcctccttag attattcagc ttcagcagca
    20161 aacaactcga ttgctttaga atcagctgta agtgtgatga tagctttttt agttttgtta
    20221 gtaaaaccag tgtaacgtcc aacacgttta gcttttggtt ttacgttgat tgtgttaaca
    20281 ttggcaactt taacaccttc gaaagcagct tcaacagctt gcttgatcaa aagtttgtgt
    20341 gcacgagtgt caacttcaaa tacatatttc cctgcttcaa gttgagccat tgagctttca
    20401 gtgatgacag gttttttgat aacatcatac aaattcatta tgcaagaacc tcctcgattt
    20461 tagagatagc tgcttgtgtg acaagaagtt tgtcgctatt tgcgatgtca agaacacttg
    20521 cagttgtagc agttgcaact ttcacgtttg gaaggttacg agctgaaaga gctgcgaatt
    20581 catttccttc ttcaaggata acaagaactt tagaatcgat gctcaatgct gcaagaactt
    20641 ttgcaaattc agcagttttt ggagctgtaa atgaaagagc gtctacagct acgaatttgt
    20701 tttcagcaac tttttcagag taaactgatt taagagctag gcgacgaact ttttgtggaa
    20761 gtttgtagcc gtatgaacgt ggagttggtc cgaagacaac accaccacca cgccattgtg
    20821 gtgagcggat agaaccttga cgagcacgtc cagttccttt ttgacgccat ggtttgcgtc
    20881 caccacctga tactgcagag cggtttttaa cagcgtgtgt tccttgacga aggcttgcgc
    20941 gttggctgat gattacatca aacacaactg attcatttgg ttcgatacca aatactgcat
    21001 cgctaagaac aacttggcca gcttctttac cagtttggtc aaataatgtt acgtttgcca
    21061 ttgtgactga tttccccttt ctttattatt taccagcttt aactgctgat ttgatagtga
    21121 taagagattt cttagcacct ggtacgttac ctttgataag gataacgttc ttttctggaa
    21181 caacttgtac aacttcaaga ttttgaattg ttacgcggtc accacccata cgtcctgcaa
    21241 ggtttttacc tttgaatacg cggttaggtg caacaggtcc catagaacct ggacgacggt
    21301 ggtaacgaga accgtgagcc attggtccac gtgattgtcc gtggcgtttg ataacacctt
    21361 ggaaaccttt acctttagaa gtaccagtta cgtcaacaac gtctccagct gcgaatgttt
    21421 caactgtaat ttcagcacca acttccaagc cttcaacgtt tttgaattca cgaatgaagc
    21481 gcttaggagc cgtgttagct ttcgctacat gtcctttagc aggtttgttg ctcaatactt
    21541 cgcgtttgtc atcgaaacca acttggatag cgttgtatcc gtctgtttca acagttttaa
    21601 cttgaagaac aacgtttgga gttgcttcaa taactgttac agggatcaat tcgccagctt
    21661 cagtgaagat ttgagtcatt cccacttttt tccctaagat tccttttgtc atgagaaaat
    21721 agttcctttt ctatattttt tattcaaaaa gtttttaacg agcgtttttt atgctcaagt
    21781 ctaagataca aagggcgtca aactcaacgt gaaaatagga aactgacaca gtgtctagca
    21841 gacgcgagga agtttatctt tttcacaccg agtttagccc gtgttcaatt agattgaaac
    21901 accagcctct gctcttttat attttagatt aaagtttgat ttctacgttt acaccacttg
    21961 gaagatccaa tttcatcaag gcatcaactg ttttttgagt tgggttaacg atatcgatca
    22021 aacgtttgtg tgtacgcatt tcaaattgtt cgcgagagtc tttgtatttg tgagtcgcac
    22081 gaatgattgt gtagaggcta cgttcagttg gaagtgggat tggacccgca acttgtgcac
    22141 ctgtacgagt agctgattct acgatttttg cagccgctgt gtcaagcgta cggtgttcgt
    22201 aagctttcaa acggatacgg atttttttgt ttgccatctt tttctccttt tcgtctattt
    22261 aagataatag gctagctcca caagaaaacc gacgcggtgt tgcgtggcaa tgcaaccgag
    22321 cgtgtcgcaa cctcttgcat caaaactaag gctgtaattt acagcaccat aatagaataa
    22381 cacaaatccc ctgcgattgc aagggatttg ttaggttttt tcgttttttt gggatgaaac
    22441 tgttttccta ctttttcttt ctaaaattag ggattttctt ctatatttct tctatacata
    22501 agttaagtcc tcttgacatc aaatttatct tctgattggt ccactaagat atccgcctta
    22561 gactcagttt ctttatagta tcgcagatac tgctcccgtc tcatctgatg gctagccaat
    22621 ataaaggatg catcgcgatt tctcacagtc gtatctctag caaggcgtcg ctttaattcg
    22681 gtctcctcat ccgtgtagaa acagatggtt ttttcaaaga gttccttggg tagaaaacca
    22741 acagacatcc cttcgacaat caaaattggt ttggctccag acaagacctc actagccttc
    22801 caaggttctt caattgtcaa gacatccata cccgcctgca aggcaaggat atctctctgc
    22861 aaactctcca gttcatgcgc cactggcaga ctggctgtca ccttttgatt cggcgcgtcc
    22921 ttgggtacta ccaaatagcg tccggaggtg atatagggat ctgtttctag caaatttact
    22981 gtagtagaat ctaaagcttg gtacaattcc tgtgcaaagg ttgatttacc tgaagctcca
    23041 tgaccgtata ttcccagtgt cctgactttc cccgtctcga tctctgagac tagttggtct
    23101 actaagtctt ttttcttcat tctctcttca cctgttcata gctttctttt ccgtcattga
    23161 gcttgtccca aatcactact tgcccacttt tgagcgattt ttgcacatcg ataattcgtt
    23221 ggttagatga acctcgaaac tggagcataa gatttctctt agttcgatca tatcttccat
    23281 cgacaagaat gtcaatcagt gacaagaatt ccagtttatc tggagtttcc aacatcattt
    23341 cttcccaagt gtagccggtc caggaccaga tgtccttgtc tggcaattcc ttccgaatcc
    23401 gcttaacaag tggcaagaga atcccagtat taagaaaagg ctcccctccc aacaaagtca
    23461 aaccttgaac atagggctga gcaagatctg ccatgatctg ctcttctaat tctgctgtat
    23521 agggaatgcc agcattaaaa gaccaagtcg caacattata acatccctcg cagtgaaaca
    23581 tacagcctga tacatagaga gagttacgca cgccttcgcc gtccacaaag ttaaaggcct
    23641 tgtagtcaat gatacgacct tgactaagtt cctcgctttt ccattcttgt ggttttggat
    23701 tattcattcg ctacctctat ccaataacgc tcgactccat tgtgagcatc ctcaaatatt
    23761 ccaccatttg ctagaatgac tgctctgcta gcaggattat tcacgctaca ggtcaccaga
    23821 gctttcttga tgttcttttc cttagcaact tgcaagccct gacggagagt ctcttttgca
    23881 taacccttgc ctctttcaga tggacggatg gagtagccaa tgtgcccacc ttcttctagt
    23941 aaaaagttac tgaggcgcaa ccggagatta agaaatccaa ctgcttgacc tttctcagaa
    24001 aaagccacta actgaattgc aggaacccat ccttcaggca gattaatccc catttcctgt
    24061 tcctgattgc tttccaacca ctcttcatac acaaaattct ctgtatccca aaatcctcca
    24121 tcatgggctg attggctctt ttcaaactct gtcatcatat ctaaaacagc tttcttatcc
    24181 gctaatcttg gtctgcgtaa ttccatccct acctcccact atgagaaaag cgagagctgg
    24241 ctctcacttt tattttttct tgttcttgtt taaaaattgt tctctgaagc taagcaagcg
    24301 ttctttctta ccagctccac ctttagagtg cttctcgtga taacgggtca cttgtgcgcg
    24361 ccccttatcg tctagttgat attttcccat ttcatttctt tctaatttgt tacttgatgc
    24421 ccagctattt taatcgttga accattcata tgtttgacac gcgcagcgat ttccttgtga
    24481 cgcccgttaa ccattggtct cgcttgagga ttgccaagat agccacaagt ccgtttaaca
    24541 acgtctactg ttttaggatc gctattgcca cagtttggac aagcaaaccc tctctcagtt
    24601 ggttcaaaat ccccttcaaa gtcacacttg tagcaacggt caatcggagt attggtgcct
    24661 agatagccta cacggtcata agcataatcc cagacagctt ccaaggcctt tggattttgc
    24721 tgaaggactg gatactcaca ataatggatg aaaccacctg acgcacctgc ttctggatag
    24781 actttctcaa agtccaattt ttcaaacggt gttggatttt tacgaacatc gtagtggaaa
    24841 gagttggtgt agtattcctt gtctgtgata tcaggaatag agccaaactt gtctatatct
    24901 agtcggcaga aacggtctgt cagactttcg gatggtgttg agtagataga gaaatggtag
    24961 ccatattggt ctgaccactc ttctacacgg cgtttcatat cgtgaatgat gtctagcgtg
    25021 aattccttag catctggatt actttcccag ctgttaccaa agaaaactgt cgctacttca
    25081 tacaagccga tatagcccag cgaaacggtc gcacgacgat tcttaaagag ctggtcaaca
    25141 ctttcttctt tacctagacg atggccaaaa gcaccgtact gataaagaat aggagcattc
    25201 gctggtgtcg cctctttagt gcgttcgaca cggtaaacaa gagcatcttc tgcgatattc
    25261 attcgctcgt tgaagatttc ccagaactta ttcatatcac cttcagactc aagagcaata
    25321 cgaggcagat taaccgtcac aacacccaga ttcatgcgac ctgaattgac ttctacacca
    25381 ttttcatcct tccacccttg aaggaaagaa cggcagccca taggaacttt gaaagaacct
    25441 gtcaaatcaa caatcttatc ataagacaag acgtctggat acatccgctt ggttgcacac
    25501 tctagagcca actgcttgat gtcatagttg ggagttcctt cctctaagtt gaggcctctt
    25561 ttaagcgtaa agataagttt aggaaagata gccgtacggt gttctgaacc aagacccttg
    25621 atgcgaatgt ttaaaatagc tttttgaatt tctcgttcaa aacgactggt tcccagacca
    25681 aaacctaacg aagtaaaagg tgtttgtcca tttgaagtga agagagtatt gatttcatac
    25741 tcaagagatt gcatggcatc gtagatgtcc ttttgcgttt tcttccaagc gtaatcttcc
    25801 cgtttttcag gcaataccca ctcttctgca tctttgagat gtttttgata gttcttctct
    25861 gcataaggcg ccaaaatttc atcgatacgg tcagctgaac agccaccgta ctggctagaa
    25921 gcaacgttgg caatgatttg agaaatctgt gctgtcgcag tctggataga cttgggactc
    25981 tctacctctg catttccaat cttaaaacca ttttccaaca tacccttaaa atcaatcaaa
    26041 cagcagttgg tcataggggt atagggactg tagtccaaat cgtgatagtg gatatccccc
    26101 ttttggtggg cattggctac gtgcttagga agcatttgca gtccgattga tttcccaaca
    26161 atccctgctg tcaaatcacg ctgagtgtta aagacatcac tgtctttatt agcattttca
    26221 ttgacaactg tctggtcttt gttgagaagt ttatgaatac taaagttgat atccgtcgct
    26281 tttgagcgct caaaatccct ctgtgtccga taagtaatat actcctcagc cagcgcatat
    26341 tctttggctt caaggagttc atgttctacg atattttgaa tttcgtaaat cttaattccc
    26401 tgtggaaagc gactatgaat ttctgtaata attcgctcag tcagatcatt gaggcgtttt
    26461 tcaaccaggg gtgtcacatc cataaccttg tcagccgcct tgtggagagc cttgtcaatc
    26521 ttgtccacat caaatacaac acgtctccca tctcgtttct cgacgaagag agttggcaaa
    26581 attgtaattt tttcttctag tgcaatcata cgcatgctct tttctaaaat gttctttcta
    26641 aaaagtatta taccactcta aaaagaaaaa tcaatatctt gtgttaaaaa tcctaaaatt
    26701 tttaaaaata cacaagatat tgatttggtt atttaatttt atagacttta aactctgagt
    26761 aatcagtctt tacttgttgg taattttctt ttaaaagtgt ttccacttca gaccagacta
    26821 ccaccttgtc atttaccaca atcaccttag gttgtttttc tttcaagtca ttaagtaact
    26881 tattacgatt ttcctcagtt gctgtatagt gcaacgggga caaaatcgcc gatggcgaca
    26941 agcgctcact cttacgataa agagtcgcag tatcatccca tgcatagata gcatcttccg
    27001 tatttgtctc ctcttttacc aaatcagcaa gacgattacg ttcttgataa ggtactggat
    27061 aaaggacaaa cttcatcaaa aaaggaacag acaaaagata gacaaaccca aaaactagca
    27121 gatatagatt tcctttgaag aaacgactcc ataggctaac tggcgtttct cttaggcttc
    27181 ttctagcact acgccccctc tgttgactct taatattggt taccaataaa agtaggaaaa
    27241 cagggaagac gaccatcaaa taagaagcgt gcagaggctc ttgtgaaaag aaaagtaaaa
    27301 tcaaacctaa aattaccaca agacttgcag gaaccgaaat gacgtatagt ttaaagggtt
    27361 tggattgaaa gagacctaaa aagatacaaa ccacaaatcc caaaccaagg gacaacaagc
    27421 cataaaaaaa catattctct aaaattttag aagtaaaatc aaagcgaata ctgtcaatag
    27481 gataacgaat accactaatc gcatccccaa aacttcctgt tgcagcacta tagtaggcag
    27541 ttggataaaa gacaagtgaa aaacctaaag ccactgcaag aaactgataa aacccatgcg
    27601 caaagcgtct atgcccaagg ttaaagacca acaagcctaa actcactaca gcaataaaca
    27661 ggagcgatga taagggagca aagaaaaatc cgcctgccaa agctagccca atccgtacaa
    27721 atcccttatc atggcttgga ttgcttaggt aagccgcaac taaactaaag gctgcgaata
    27781 agaaaggaag cgctaaaaga gtcgcatagc ctccaccaaa agcaagacct gtaactagca
    27841 tgtaaaaaat agtcaccaga tgtccagctt ggtctccttg ctctgtcaag gtgtccgctg
    27901 atctaaaaag gaaaaatcct cctgctacca aggctaacca ctcaaagatg gcaaagaaaa
    27961 atccgccctg actcacgtaa gtcagcaaat aataaagcaa accttgactt ccataatagt
    28021 cgctgtaaat cttccctgtc tgatgaagcg cccaacctgc ataaaaatcc tgcacttctt
    28081 gtgcactcat taagtcgagt aatagcggta ctcctagagt tatccccgtt acaagcgtac
    28141 tccatagtaa aattttcacc aaaggaagac gacttgattc acgattatgc gattcttgtt
    28201 cgatttggta ttctagaggt tcacgattct ccttatgaac ttcttctact ctaccataca
    28261 cactcatatc gtttctcctg ttcaatttat ctgtcttagt atatcaaaaa gtcctaggat
    28321 tgtcagcttg cgatggctgt ttgagtgatt ttttgcatag tttcacaaag gtttcaattt
    28381 cccgaaagcg gtgtaaatgt gtctgtttga actcaatcat atcattcact tcttccttag
    28441 ttagcatgaa ttcccctcct ctttctttct attcgtaaaa gacaagaaaa ataggtctgg
    28501 tttgtatcct aagttctaga tttcttataa gataggggcg aatagctggg cgatttcttt
    28561 tacaagcttt tgataccagc tcgttttgat agaatgagga tagacttcct gagaatctgc
    28621 aaatatattt cgaaaatctc gggcgatttc ccttatagaa ggagttttat agagtaagac
    28681 tgcattttca taatggtgta ccaagctccg gtagtcgaga ttgattgtcc ccaccaccgc
    28741 aaaatcttcg tctaccaaca tctgcttact atgaataaaa cctggactat actcataaat
    28801 ccgaacacca gcagaaagaa ggtcgggata agctcctctc gtgactaact gaatgaactt
    28861 cttatctggt atgtaagggg tgataattcg aacatcgacc cctctcatag ctgcattttt
    28921 gattgtctct gttaaatcat aatctataat caaataaggt gtcgtaatat agaccgattc
    28981 tgttgcttga ttgattaaac tctgataaac ttttttccct acctgcgctc gaaaaattgg
    29041 cttgggtcca cttccgtatg gaatggttaa accgtcactc gggatagaat gattttctaa
    29101 atgatattga tcaaaatcac taatttctcc tcgattaatg taccaagtgg tcaaaaataa
    29161 gcgtgtcagg gcttttactg ctagtccgtc taagcgaatt ccactatcct tccaataacc
    29221 aaatctctcg acgtggttaa tgtactcatc tgccagattg accccaccag tataggctat
    29281 ctgaccatca acaatcaata tttttctatg atctctgtta ttataagcca ctgtcagacg
    29341 aggaataact ttattgaatt tatgggcctc gatgcccagc tgacgaagtc gatgtgcata
    29401 atctcctgtt aaagtagcca tacagccgat atcatcatag agcatcttaa cctctacacc
    29461 ctgagctacc ttttgctcta gtatatctag tatgcgattc cacatcaaac cttcttctat
    29521 aatgtaatat tccaagaaaa taaatttctc agccttttta agatcttcta ccatcttttt
    29581 ccacatagct tctccgttag gaaaaaatgt agaggcagtt tgatcataga tgtcagcatt
    29641 ggtatccata ctcaataagg acttgatgac tccataagct gccttgtcac tttcttttaa
    29701 tttctctttt agtagctggc tatttgcttc ttggaaatgc atagagccta gcttcttcag
    29761 ttgtttgatt tcttttttgg acaatcgcct ttcaccaaac atcagataga gcaagggacc
    29821 aaatactggc acaaaggcta ctaacaacca ggttacctta ttctcaggag tcgtattacg
    29881 gttgactatc gaaatgattg tgatgatact cagtagaatg aggacagtaa tccataaaat
    29941 gggagccatg cgtcctaaat aaagaaagag accaaaaaca agacaaagtt caagtagcat
    30001 aatcgaaaga ctaaagccat acttggacat caataattga aatttcctat atttcatatc
    30061 ccctccttga tagtttgaat gctaaaaaca agtaattgat actagtataa cgcaggtatt
    30121 cggtagaagg caagtattta tgattatttt ctaggtaaaa gtaaaaaggc tgaaaaatcc
    30181 agccctttct acttgcatag tcttatttag ttaaaacgat ttggtccgat gggtctctat
    30241 ccatatcatc tacgacgatt tgattaccgt ttacagtgta aattttgaca tcatcgccaa
    30301 taatcatgcg ttgatttgct gaatcaaatg taacctgttt gactttctga tccccgtcag
    30361 attcgagctc agtccatgta ccagtcgttc cagttaccac taaagtgata cggtctccgt
    30421 catcctgacc agtataagtc ccatcaatat cggtcggttg agcaacaggt tggttttgta
    30481 ctggagccgc ttgattttgg ttacctcctg catttgcagt attggtattc tgttgagcag
    30541 gttgttgagc aggctgttga acctgttgag ctctttgtga acaagctact aagagactaa
    30601 aacttgctag agaagcaaga gaaagtgtga atctttttaa tttcatgatg aatttccttt
    30661 ctgctaccaa tttagagaaa ttttctctaa ccagcaattc ccctagtata acaagttcaa
    30721 acaagggggt caatttatct gctcacggtc cagcaggtag ccccgtactt ctgagataaa
    30781 atagagagac cctgtaacga acagcaagtc ttgagcgtct gccctttctt cgaaaatgct
    30841 gataaattct cggtaagagg gaattacatg gtaacctgtt acatctgtct cgtccaaaga
    30901 cccttgatag tcaaagccag tcattttgag ttccacttga ggtaaatttt cagtcagata
    30961 acccaacatc ccttgataat ccttacgttt caaggatcca aagaggattt gaggtcgata
    31021 accttcctgc tctttttctt tgataaactc aaccaagcga gtcaaggcag ggaggttatg
    31081 agcaccatcc aagtaaatct gtgggcgaat acgctccaag cgaccagccc aatgggtctg
    31141 ttccaaggcc tttcttacag cctgttcatc aacagcttcc tttccttctc ccataaaaag
    31201 aagaaaagtt tgcaacgcca aggctgcatt ctcctgctga tagactcctt ctaagcctat
    31261 gttcagctgt gaaagattta gtaaagagct tgaaaaatca ccattcagca ttaaaaaatc
    31321 ttgacctgcc tgataaaggt taacagctaa agattcggct tttttctgac agacaagcct
    31381 agcttctgga ggcaatttcg caatcactgc cttcttacca gccttgaaaa taccagcttt
    31441 ctgctctgca attgcttcta gactatcacc caaggtttct tgatggtcaa gcccaatgga
    31501 ggtgatgaca gcaagctctc cagttaccac attggtcgtg tcaagtaagc caccaattcc
    31561 cacttctaat aaaaccaaat ccacctcttg ctccctaaaa taaagaaaag caactaaggt
    31621 cagcaattca aaaaaggaca actggtcagg agtttgcaga agcgttttct ccatctcctt
    31681 gacctgatca gccaaacgga taaagtctgc gtctgctata ggttgcccat taatgcagat
    31741 tcggtcattg atagagacga tatgagggga ggtaaaggtc gcaacttttt tgccatgccc
    31801 cataaataat tccctcataa aagcaatggt agatcctttt ccattagtcc ctgttacgtg
    31861 gataataggg taagactgct caggatttcc taacaaatcc accgcttgct gtattcggcc
    31921 caaacctgat cgaaaattca aaccaatccg actatggagc cattcttcta cttcaaacat
    31981 acacatctcc ttgacaaaag tccaatcaat tatcgcatta aagtatggtt actaataaaa
    32041 acaaggccag gattttcgtc ccgacctctt acctggttag ctaataacta gctactatga
    32101 atgtgaatat gggctaaaaa catccactag atgttccaac tcttctcaat ttctaagagt
    32161 tggggtaaaa atgttcactg gacgttccaa ctcttcccca tttctgggag ttgggctgat
    32221 acagtttctc agacttacca gctctatctt atttcaagga gctgggctaa aacatccccc
    32281 gaacttactg actgttttta tttttagcgt caggctaaaa acgtcccccg gacttaccag
    32341 ttctctctta tttaaaggaa ctgggctaaa aacatccact ggatgttcca actcttcccc
    32401 atttctggga gttgggctga tacagtctcc cagactgcct gctctttctt ttttctaaaa
    32461 gcagggctaa aaacgtcccc ggacttacca gctctctctt acttcaagga gctgggctaa
    32521 aaacgtcccc cggacttacc agttctctct tatttaaagg aactggggta aaaatattca
    32581 ctgaacattt ttactcctcc ataaaactat tgaagacttc ttcaatcatg ttccattcgt
    32641 cttctgagtc ttctgggatt ggttgcaatt cgccttctgt tccatcttcg ttttcgatga
    32701 atgagtaagc ttggatttca acttgtccgt cttcgtcttc ttctgcgtta actggtacta
    32761 gaagaacata gtttttacca aattcttctt ttccatcaat tgtcaaaagg atttcaaaca
    32821 aggtttcatt tccttgctca tctactagtg tgattagttc acgttcttcg tggtcgtggt
    32881 tatgatcgtg tgacatagcc tctcctttat attaaaattt tctatctaaa taattttgta
    32941 aaatcagctg agctgctaac ttatcaatga ctttcttgcg cttattgcga ctgatatctg
    33001 cttgttcaat caacatgcgc tcagcagcca ctgttgtcaa gcgttcatcc tgatagtcta
    33061 ctggtaaacc aaaaaactct tctagctttg ctccgtaggc ttgactagct tctacgcgcg
    33121 gtccacttgt attgttcatg tttttaggca agcccactac aaatcgttcc accttgtaag
    33181 tatcaaccaa ttccttaacg cggtcaaaac caaattggcc ttgttcttca tttatctgga
    33241 tgatttcaag cccttgagct gtaaaaccaa gcggatcgct aatcgccacc cctaccgttt
    33301 ttgaaccgac gtccaatccc ataattctca taggttatag atcgactcct tgtcctttga
    33361 ggtagtagcg aaccaattcc tcaacgattt catcacgctc atacttacgg atttgatttc
    33421 gtgcattatt ataacgagga acgtaggcag ggtctccact caatacgtaa cctacgattt
    33481 ggttaattgg gttgtaaccc ttatcgttca acgaagcata aacatctgtc aaagtttcgc
    33541 taatttcttt tttattggaa tcgtccaatt taaaacgtac tgtttcttca gtaaatccca
    33601 ttctaacacc ctctttcctt agaatagtac cattatagca taattcctta ccttctacaa
    33661 ttcaggcagt ctatttattt ggattttcta ttgttctgtc gcgccatttg ccaatctatc
    33721 tgaaatatat ttgcttggtt catttttcaa aagattttcc aaaccaatat tcttcagatg
    33781 ttccaactgg gaagccttct tgacatccag aacttgaaaa tcaaaactag tcgttgtttg
    33841 aagttccgtt gcgctcaata gttttgtttc aagtttgaaa cctgccaatt tacgagcttc
    33901 aatgatagac ttatccttct cctccgcttc aagaagagct ttttgagttt cctccactcc
    33961 atgttggtca atataagtct tcaactcatc agagactgga cgtttttgtt ggatagttag
    34021 actcaaaaaa gtcttgtctt tgtaagtaat tgtttgtgtc tgctggctac catcatctga
    34081 cttgggcaag accaaagtct tggttacaac tgtattcttc tcagcatttt caataactgg
    34141 caatgccgac tgaagcgtat ctttttctgt ttttgtagct ggtccagttt cttttttctg
    34201 tccgcaacca accaggacaa aaaggaaagc tagactaaca agaactattt ttttcatttc
    34261 tttcttcttt ctttttgaaa ttaaaataga ataagactgg gaagtgctcc cagccttgat
    34321 gtttatagag ctgcacgcaa acgtgcttct gcattttcta cattacggac agagcgtggt
    34381 aggaaggcac gaatatcgtc ttccttgtag ccaacttgca ggcgtttttc atctacaagg
    34441 attgggctct ttaaaattct cggtgtttcc ataatcagat tgagaacttc attgacactc
    34501 aaatcttcaa tatccactcc aagggctttg gcatagcgat ttttagacga aacgatgctg
    34561 gctattccgt tatctgtttt ggttagaata tccagtaatt cttctctcgt aattccttct
    34621 ttaccaaggt tttgttcttt ataacttaac tggtgggcat tgagccaggt ttttgctttt
    34681 ttacagctag tacaacttga gactgtataa attttaatca tgtacctacc cctttcgcta
    34741 catgttacta tcagtttagt ctattatacc ataaaaaaca tccgacttgc gacctatttt
//
LOCUS       DAYYQN010000015        34737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYYQN010000015 DAYYQN010000000
VERSION     DAYYQN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.015"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            259..1110
                     /locus_tag="ACHBRQ_000681"
                     /note="rgg1518"
     CDS             259..1110
                     /locus_tag="ACHBRQ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator
                     Rgg1518"
                     /protein_id="HFM9592392.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(1342..1692)
                     /locus_tag="ACHBRQ_000682"
     CDS             complement(1342..1692)
                     /locus_tag="ACHBRQ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592393.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LRKSKGVHKGRCRN"
     gene            complement(1832..2167)
                     /locus_tag="ACHBRQ_000683"
     CDS             complement(1832..2167)
                     /locus_tag="ACHBRQ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592394.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(2258..3568)
                     /gene="der"
                     /locus_tag="ACHBRQ_000684"
     CDS             complement(2258..3568)
                     /gene="der"
                     /locus_tag="ACHBRQ_000684"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9592395.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(3582..4295)
                     /locus_tag="ACHBRQ_000685"
     CDS             complement(3582..4295)
                     /locus_tag="ACHBRQ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HFM9592396.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(4292..5188)
                     /gene="dnaI"
                     /locus_tag="ACHBRQ_000686"
     CDS             complement(4292..5188)
                     /gene="dnaI"
                     /locus_tag="ACHBRQ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9592397.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(5189..6358)
                     /locus_tag="ACHBRQ_000687"
     CDS             complement(5189..6358)
                     /locus_tag="ACHBRQ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9592398.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQII
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(6359..6832)
                     /gene="nrdR"
                     /locus_tag="ACHBRQ_000688"
     CDS             complement(6359..6832)
                     /gene="nrdR"
                     /locus_tag="ACHBRQ_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9592399.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            6972..7337
                     /locus_tag="ACHBRQ_000689"
     CDS             6972..7337
                     /locus_tag="ACHBRQ_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9592400.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            7342..8037
                     /locus_tag="ACHBRQ_000690"
     CDS             7342..8037
                     /locus_tag="ACHBRQ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592401.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            8054..8821
                     /locus_tag="ACHBRQ_000691"
     CDS             8054..8821
                     /locus_tag="ACHBRQ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592402.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(9233..10432)
                     /locus_tag="ACHBRQ_000692"
     CDS             complement(9233..10432)
                     /locus_tag="ACHBRQ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592403.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(10429..11322)
                     /locus_tag="ACHBRQ_000693"
     CDS             complement(10429..11322)
                     /locus_tag="ACHBRQ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592404.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQDQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11444..11635
                     /locus_tag="ACHBRQ_000694"
     CDS             11444..11635
                     /locus_tag="ACHBRQ_000694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592405.1"
                     /translation="MKSKEQTRKLAVGCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYIYGKAKLTWFEEIFEEY"
     gene            12003..12216
                     /locus_tag="ACHBRQ_000695"
                     /pseudo
     CDS             12003..12216
                     /locus_tag="ACHBRQ_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            12631..13248
                     /locus_tag="ACHBRQ_000696"
     CDS             12631..13248
                     /locus_tag="ACHBRQ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFM9592406.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(13568..14455)
                     /gene="scrK"
                     /locus_tag="ACHBRQ_000697"
     CDS             complement(13568..14455)
                     /gene="scrK"
                     /locus_tag="ACHBRQ_000697"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9592407.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVVRHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(14604..16487)
                     /locus_tag="ACHBRQ_000698"
     CDS             complement(14604..16487)
                     /locus_tag="ACHBRQ_000698"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9592408.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     SQGNKVKAGDVLGKFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            16864..18318
                     /locus_tag="ACHBRQ_000699"
     CDS             16864..18318
                     /locus_tag="ACHBRQ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9592409.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGIYYELDYGHKTN"
     gene            18299..19264
                     /locus_tag="ACHBRQ_000700"
     CDS             18299..19264
                     /locus_tag="ACHBRQ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9592410.1"
                     /translation="MVTKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(19299..20573)
                     /locus_tag="ACHBRQ_000701"
     CDS             complement(19299..20573)
                     /locus_tag="ACHBRQ_000701"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9592411.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGASDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(20573..21745)
                     /locus_tag="ACHBRQ_000702"
     CDS             complement(20573..21745)
                     /locus_tag="ACHBRQ_000702"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9592412.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(21876..22124)
                     /locus_tag="ACHBRQ_000703"
     CDS             complement(21876..22124)
                     /locus_tag="ACHBRQ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592413.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(22192..22815)
                     /locus_tag="ACHBRQ_000704"
                     /pseudo
     CDS             complement(22192..22815)
                     /locus_tag="ACHBRQ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(22877..23326)
                     /locus_tag="ACHBRQ_000705"
     CDS             complement(22877..23326)
                     /locus_tag="ACHBRQ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9592414.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(23328..24086)
                     /locus_tag="ACHBRQ_000706"
     CDS             complement(23328..24086)
                     /locus_tag="ACHBRQ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9592415.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(24201..26180)
                     /gene="stkP"
                     /locus_tag="ACHBRQ_000707"
     CDS             complement(24201..26180)
                     /gene="stkP"
                     /locus_tag="ACHBRQ_000707"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HFM9592416.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(26177..26917)
                     /locus_tag="ACHBRQ_000708"
     CDS             complement(26177..26917)
                     /locus_tag="ACHBRQ_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9592417.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(26932..28245)
                     /gene="rsmB"
                     /locus_tag="ACHBRQ_000709"
     CDS             complement(26932..28245)
                     /gene="rsmB"
                     /locus_tag="ACHBRQ_000709"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9592418.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(28238..29173)
                     /gene="fmt"
                     /locus_tag="ACHBRQ_000710"
     CDS             complement(28238..29173)
                     /gene="fmt"
                     /locus_tag="ACHBRQ_000710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9592419.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(29186..31582)
                     /locus_tag="ACHBRQ_000711"
     CDS             complement(29186..31582)
                     /locus_tag="ACHBRQ_000711"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9592420.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(31648..31962)
                     /gene="rpoZ"
                     /locus_tag="ACHBRQ_000712"
     CDS             complement(31648..31962)
                     /gene="rpoZ"
                     /locus_tag="ACHBRQ_000712"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9592421.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(31987..32613)
                     /gene="gmk"
                     /locus_tag="ACHBRQ_000713"
     CDS             complement(31987..32613)
                     /gene="gmk"
                     /locus_tag="ACHBRQ_000713"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9592422.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(32743..34356)
                     /locus_tag="ACHBRQ_000714"
     CDS             complement(32743..34356)
                     /locus_tag="ACHBRQ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9592423.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         9921 a         7818 c         6613 g        10385 t
ORIGIN      
        1 ttctaaacga tggtatatca tacaaattcc tcctttactt attatgtata gactctaatt
       61 tctttatccc caaaaccatg tttcaaacca gataagttga atccaactcc aaatcaaaaa
      121 tccagagttt ttcggtaaat tcttcaaata ttttttaaag cccatgactt tttccctcct
      181 ttttgatata ataataccac ctttttctac gttttttata aatgtagaaa atattcacat
      241 ttacctagga ggaacaatat gagatgggat tatggacaaa tttttaaaga aattcgaaag
      301 tcaaaaggat tgacccaaca agatgtatgt ggacaagtca tacatcgaac aactctaaca
      361 aatattgaac acggtaaagt tattcccagt tttgaaaaca tggtatttct tcttgaacaa
      421 attgatatga gcttggcaga attcaagtat atatgcaacg aataccatcc tagtaaaagg
      481 cgagatatta ttgtagagag ccaaaatccg tctacttttc aagatactag aaaaatggtt
      541 gaactcactg agaaatgtca aaaatatctt aagacacatc acgatgttcc tattcaaaat
      601 atctatcgtc atacaaaaat tgtcacagag ttacgaacta aaggattcaa aaacaaccac
      661 gtcttgaaag atttgtatga agaaatttgg gactatcttg aacctatgga tacatggtac
      721 attagtgatt tgaaattgct tggaaccatt ctctttttct ttccttctga aaatcttccc
      781 cttcttattg atagaattat gaaaaccatc gagaaatata aatacttccg agaaacaaaa
      841 gcatttttat catctttttt agccaatctc tccactgtat attttcaaca tcatttattc
      901 aaagaatgtg aaacaatcac cctacaactt ttggtattag cagaagaatt aaaaatatac
      961 gatattcttg gattctctca agtacgcttg ggaatactac aacataactc cgatttgatt
     1021 gataaaggaa taactttatt acgactaacc aaagaagagg cattagttaa aatactcgaa
     1081 aaagaaatta atgatttttc aaacctataa tttgtaataa ctctcatcat tttattatag
     1141 tagattgtaa tagaatgtaa tagaatgtga acgaatcaat taggggatct aaagcatttt
     1201 ataccaatgt tttataagtt gtattgtatt attccatatt caatccacta tacaagatga
     1261 atatttctct caagtttcat aatcaaaata aaaatgactg tatctatagc tccataaaca
     1321 aatcatagat acagtcattt tttagttcct acaccttcct ttatgcacac ctttagattt
     1381 ccttagttcc cgtctcaagt caagcatttt tttgaaacag atatagtcta accagtcact
     1441 agcaaaagcg ataagtagta ataatagcca aataccccat aacttgatat ctgtcaaatt
     1501 tctcaagacg atattgaaaa acagaactga aaaaactgtc caagcaaggc taaaaagagc
     1561 atagagggtg atgtaaatcc agtaaaaata gtaaaaaatt gggaaaaatt tactttctct
     1621 attatccttg tcaatccagc tgaaaaagta aaaacagggt aataagatta tcattttaaa
     1681 caagtgttcc atcacaagcc tcctttttta aattgttttc tttagtttcg ttctattaaa
     1741 tgttattacc aatataaacc ttggaatagc gtgtaataaa tgattttaga ggtaccataa
     1801 gccacctcct acaaatagaa accgatataa atcaatgcct tccaccctta gacttcccta
     1861 gttcctgtct caagcgaaac atttctttga aacaggaata agttaaccaa ttcataccaa
     1921 tagctagcag aataaaaaga aaccaaatgc cccataactt gatatctgtc acatttctca
     1981 agacggtatt gaaaaacaga actgaaacaa ctgtccaagc aaggctaaaa agagaataga
     2041 aggggatgta aaaccagtaa aaataataaa aaattggaaa aaacttacta tttctgttgg
     2101 ccttctcaat ccagttatca aaataaaagt acggtgctaa aagtaagaat ttaaacaaat
     2161 gttccatcac cgacatcacc ctccttcttt tgatagcgtt ttctattatt ttattatatc
     2221 aaaaaaatcc ggaactgtca ttccagattc tactttttta tttgcgtttt cttgcgatga
     2281 gatgaatcgg tgttccctca aaaacaaagg ccttgcggat ttgattttcc aagaaacgca
     2341 ggtaagaaaa gtgcatgagt tcttcttcat tgacaaagat gacaaaggtt ggtggtttgg
     2401 ttgccacttg ggtcgcatag aaaatcttga gacgttttcc tttgtctgtc ggtgttgggt
     2461 tgatggcaat ggcatccatg atgacatcgt tcaagacagc tgatggaata cgtgtatttt
     2521 gactttcgct gatttgctta atcatctcag gaagtttgtg gagacgttgc ttggttaaag
     2581 ctgatacaaa gataatcggt gcgtaaggca ggtattggaa ctgctcacgg atatcttctt
     2641 cccagttttt catagtgtgg ttatcttttt caagcgtatc ccacttgttg accacgataa
     2701 tcatcccttt accagcttca tgggcaaatc ctgcgatacg cttgtcgtac tcacgaatgc
     2761 cttcttccgc attgatgacc atcaagacca catctgaacg gtcaatagca cgcatggcac
     2821 gcataacaga gtatttctca gtattttcat aaaccttacc agacttacgc ataccagccg
     2881 tatcaatcat ggtaaactct tgaccatctg tatctgtaaa gtgggtatca atggcatcac
     2941 gagttgttcc agcaacagga ctagcaataa cacggtcttc gcccaagata gcattgatca
     3001 agcttgattt tccaacgttt ggacgaccaa tcaggctaaa cttaatgaca tctggatttt
     3061 cttcttcata ttcatttgga aggttttcca caatcgcatc tagtacatcc cctgtaccga
     3121 taccatggac agatgagata ggcagtggtt cacccaaacc gagggcatag aaatcataga
     3181 tatcatttcg catctcaggg ttgtccacct tgttaactgc gaggataact ggtttgtggg
     3241 tcttataaag cttacgggct acgtattcgt ctgcatcggt aattccttcc ttaccggaca
     3301 cgacaaagac gataacatct gcttcttcca tggcaatttc tgcctggtgc ttgatttgtt
     3361 ccatgaaagg agcatcgaca tcatcaattc ctcctgtatc aatcatgcta aaagaacgat
     3421 tgagccactc acccgttgca taaatacggt cacgtgtcac tccttcgaca tcttctacaa
     3481 tggagattcg ctcaccagcg atccgattaa atagggttga tttcccaaca ttgggacgtc
     3541 ctacaatggc aatagttggt agggccataa tttctcactt tctacaataa tttcttctgt
     3601 tcaagatttt ttctagttga gcttggttca gcttgaccaa actgttctgc taggcgctga
     3661 ctccagcttg tggtcgcacg cgccccagca tagtcagcct gaacacggtc ataagcttgg
     3721 attgcctcag ttgactgttc ttggtattct tcctcaaaga caacattctc tagtggcagt
     3781 ctcggtttca tatcatgatg ttgatttggc acacccagtg ccatcccaaa gacagaatag
     3841 gtgtagtcag gtaggttaaa gagctctgcc acttcttcag acttgtatcg aaccaaaccg
     3901 ataatcacac caccatagcc caagctttca gctgccaaca aggcgttttg tccagcaaga
     3961 gctgcatcga ccgaactaat caagagacct tccacacctt ggggttggaa ggtgtcggta
     4021 tgaagtcggg ctcccttttc tgctcggttc aaatctccga caaagagaag gaaaacagca
     4081 gactggcgaa tggcttcttg aggtaccaat tcatacaagg catctttctt ctcttgactt
     4141 cgtaccacaa tcacagagta ggattggaaa ttcttccaag atgatgccat ctgggctgct
     4201 gtcaaaatct catttaagtc tacttgggga atttcttgct ctttaaacct gcgcactgaa
     4261 gtatgagcct tcatcaattt aatggtttct gtcatcgacg gtttactcct tctaaacgag
     4321 tctcctcagc caaataacgg atgcgttcca tgacccgtct ggcttcccag gtttcgtcat
     4381 ttccatgttt cactttcgca aaatgcttct ccaaatcttc aaagttgaag ttggatgtga
     4441 aaaaggtcgg taaattttcc tgcatccgat attggagaat gacctgcagg atttcgtcac
     4501 gcacccaagc ggtggattgc tcggcaccaa tatcatctaa aatcaggact tcagaaagct
     4561 taatctcatc cactaaggtc ttaacattgc catcaccgat agcatttttg acatcaatga
     4621 caaagctagg atagtggagg agagttgatg aaacaccacg tttttctgat aaatcatgag
     4681 ctaaggccgc caccatgaaa cttttaccca caccaaagtc tccatataag taaagacctt
     4741 ttcgaatagc tggatattgc tccacgaagg ctagtagctt ttcaaaaact ggtaagcgcc
     4801 ccaaatcatc caagtcaact tgagccaaac tagctttctt gagactggct ggtagattga
     4861 ttaacttgag acggttctta atagccgctt ctttttcagc cgcgattagc tcaggagttt
     4921 cttcatatga aacatctgca tagccatgat tcttaaccaa aatcggcttg tagcctttgg
     4981 caatataatc cgtatcccca cggagaaact tgtcacgctc ggtgatgtac tgattaaact
     5041 tggagatact gcgatttaat tcctctggag tcaaggattc ttgctggata aaagccgcaa
     5101 catcagggtc cttcatgatt ttctggacca aatcttgata ataaaaacgg ctgggttgac
     5161 gtttgagtac gtctccgaca ctttccatct aatctcctcc tttttctaat cgagctaata
     5221 gttcttgctt cttacgttct agttccagac gagtttcctc gctggtttca ttcttatatt
     5281 caggattact ccatttagga acattggttt ttactggggc agtctgattc tgtttttgtg
     5341 tttttgcttt ctgtcctcgc tcacggatgc gcaagactgc cttttctgcc gaatgaatct
     5401 tttgataggc atagtcattg gctaccttca tggcatattt ctcattgata tttgccgaat
     5461 cgaccttatt aaaggtcaat aaaagaatga tattgatgac ttcgtccagc aagcccaagc
     5521 cagccatctg ttgcaagagt tctctttctg tttgggtaat ggttcccttg cgtgtttgct
     5581 tgatttctgc caagaactgc agggcagttt tacttttggc ttctttgata atggtcgctt
     5641 ccttaagact aaagtcagag gaaactggtt tttgagcaat tttttcacgc atgcgtttgg
     5701 ttgaaataat ctgagaaaca gctgttgact tggccaattg ataggtttca aaccaagtcc
     5761 atttcttctc ctcggcaata gcaaagaggt ttaagacatc ggactgctca tccgcaaagc
     5821 gaagtccatc tcgagccata agctgacgaa aatggtccaa gtcaaaatcg ttggccactt
     5881 tcttcttgag accaaggtct tcttgactgc ctagttctgc caattctgga aagacttgat
     5941 tgagtgagac aggtatttct tcaccatcag cactttcaac tttcaaatcc tccacagcta
     6001 catcgccaat ctttttctct aagagtctgc gataaacagg atgccccaag aagtcttgac
     6061 tagatagagg agcatggagg gctagctgat aaacatcccc cttttgatag agagtcaaga
     6121 gattaaaagc agataagatt ttcaatgatt ttatcagtct atccatccca aagttgagat
     6181 gattgaggat acttgaaaaa agatattcct ttctaccatt atcccaaaaa ctaatcgtat
     6241 aaagataaag gctcagtgcc tcctgaccga taatcgggag gtagcactgt accagagatg
     6301 aggtatcttg cgacacccga ttattcttta gataagaaaa acggtcaatt ggcttcattt
     6361 atctttcctt tttcttttta gaggactggg tgatttgttg gagcaagctc tctaactcac
     6421 tgacatcctt aaaactacga tagacactag caaaacgtac ataggtaatc tcgtccaatt
     6481 cagccaactc ctccatgacg agtgaaccaa tgtcctcact ttgaatttca ttttcatttc
     6541 gaccacggag tttctgttcg atacgattga ctaccatgtt gatttcatca cttgacacag
     6601 gacgtttctg ggctgagcgg ataatcccat taaagatttt atctctggag aattgttccc
     6661 gtgtgccatc ttttttaaca accactaagg ttctttcttc tactcgttcg taggttgtaa
     6721 aacggtgttg gcattcgtcg cactcacgtc ttctacgaat ggtgttccct tcttctgctt
     6781 ggcgactatc gataacactt gacttggtag ccccacattt tggacaacgc atcctttccc
     6841 tccttatcgt tttcttttca ttataccatt ttttaagcga ttcccaaaac aattcttctt
     6901 tttgcttgac aagttttttg ttttgttgta ttatttagtt aagacaaaag gataagagaa
     6961 aggagactaa gatgtcctgg acatttgaca acaaaaaacc catctattta cagattatgg
     7021 agaaaatcaa gcttcagatt gtttcccata cactggaacc caatcaacaa cttccaaccg
     7081 tgagggagct agctagcgag gctggtgtca atcccaatac catccaaaga gccttatcag
     7141 accttgaacg agaaggattt gtctacagca agcgaacaac tggacgattt gtgactaagg
     7201 ataaggagct aatcgctcag tcacgcaaac aattatcaga agaagaattg gaacacttcg
     7261 tttcctccat gatccatttt ggctatgaaa aagaagaact accaggcgta gtcagtgatt
     7321 atattaaagg agtttaagcc tatgtcatta ctagtatttg aaaatgtatc caaatcatat
     7381 ggagcaacac cagcccttga aaatgtttct cttgacattc cagctggaaa aattgtcggc
     7441 cttcttgggc caaacggctc aggaaaaaca accctgatta aactaattaa tggcctctta
     7501 caaccagatc aaggacgtgt cctcatcaac gacatggacc caagcccagc aaccaaggcc
     7561 gttgtagctt atttgcctga tacgacctat ctcaatgagc aaatgaaggt caaagaagcc
     7621 ctaacctact tcaagacctt ctataaagat ttcaatcttg aacgcgctca tcatctactt
     7681 gcagacctgg gcattgatga aaatagtcgt ctcaagaaac tatcaaaagg aaacaaagaa
     7741 aaggttcaac tgattttggt tatgagccgt gatgctcgtc tctatgtttt ggatgaaccc
     7801 attggtgggg tggatccagc agcccgtgct tatatcctca ataccattat caacaactac
     7861 tcaccaactt ctaccgtttt gatttctacc cacttgattt ctgatatcga gccaatcttg
     7921 gatgaaattg tcttcctaaa agacggaaaa gtcgtccgtc aaggaaatgt agatgatatt
     7981 cgctacgagt caggtgaatc cattgaccaa ctcttccgtc aggaatttaa ggcctaagca
     8041 aaggagatta tttatgtttt ggaatttagt tcactacgaa tttaaaaatg ttaacaagtg
     8101 gtatttagcc ctctacgcag ccgtgctagt cctttctgcc ctcatcggaa tacagacaca
     8161 aggctttaaa aatctacctt accaagaaag tcaggctact atgctacttt ttctagctac
     8221 agtctttggt ggcttgatgc ttacacttgc gatttcaacc attttcttga ttattaaacg
     8281 cttcaaaggt agtgtctacg accgacaagg ctatctgact ttgaccttgc cagtttctga
     8341 acaccatatc atcacagcca aactaatcgg tgcctttatc tggtcattga ttagcaccgc
     8401 tgtattggct ctaagtgctg ttattattct ggctttaaca gctccagaat ggattcctct
     8461 ttcttatgtg attacatttg tagaaacaca tctccctcag atctttctta caggtatatc
     8521 cttcctacta aataccattt caggaatcct ctgcatctac ctggctattt ccattggaca
     8581 gcttttcaat gaataccgta cagcactcgc tgttgcagcc tacattggta tccaaatcgt
     8641 cattggattt attgaacttt tcttcaatct tagttctaat ttctatgtca attcactggt
     8701 aggactcaat gaccatttct atatgggagc aggtatagcc attgttaaag aactcatatt
     8761 catagctatc ttttatctcg gaacctacta catcttgaga aataaggtta atttgcttta
     8821 aataattttt acctagatat gtaacatact catagaacaa aagagaccag gcaaaaagtc
     8881 tttaaaatta gaaaacgcat agtatcaagt gttgaatatg aactgccccc aaaagttaga
     8941 cagaaaaaat ctaacttttg gggggcagtt caaaaaatcc ttgatattat gcgttttttg
     9001 tgagctgact tatttccttt cactatatcg caaaatgaaa taagaacgga acgatgggat
     9061 tttggaattc aaatcaattt ataagaatgt tttagaagta atattatcct attccagatt
     9121 cagttcacta tacaattgag ttttcaagca acctgtttac ataatgtgta cataattagg
     9181 ttcgtgattc cacccttttc acctttaaaa acctcgcttt cgcaaggctc ttctatttat
     9241 aagataaggc acgtttaaag gttttccaaa tccctaaatc atccgtttga agaacgagac
     9301 tagcatacat gcgtccgata aatcctgttg ctaccaccgc aaaaatcact gtaatagcaa
     9361 gtgaaatcca tgcttctgct ccccccgcat agccattaat cgttcgaaac ggcataaaga
     9421 aggtcgaaat aaagggaata taagaaccaa tcttcaagag gagattgtca ccagctgcac
     9481 ctagagctgt cactccaaaa aaaccaccca taatcaaaat catcaaaggc gacaaggctt
     9541 tccctgagtc ctcaggacga gaaaccatag atcctaggaa ggctgccaag actacataca
     9601 tgaaaagact gatcaaaata aagagcaagg tattcagtga gatagcatct cccaagtgat
     9661 ccaaaatacc agactgagcc aagaatggca aatctttaaa gagcaaaacg gcagccagac
     9721 cacctacaac atagatccca atatgcgtta aaatcactag aaacagagcc atcatccgcg
     9781 catagaaata gtgacttgcc cttatgctag aaaaaacgac ttccataatt ttggtgcctt
     9841 tttcactggc aacttcctga gccgttacac ccgcataggt aatcagaatc atataaagaa
     9901 agaatcctaa ggcacctgct gcaattgttt gaataaactt tttattttcc ttggcttcat
     9961 caatcttttc tgtgaattga attgtctgcg ctaagcgttt ttcctgctct tgagacaagg
    10021 aagcagttga acgattaagc tgattttgca gttcattgag tgtacctgta acctcaaatt
    10081 taattccatt ttcaagcgat gtttcgccat gataaactgc ctttagaaca ctatcttctt
    10141 gatcaatggt caaataacct tttaattttt cttctttaat tgcttctttg gcacttgctt
    10201 catctttata gtcgaagtta acaccattta cattcttcag tccttctgct acagatggca
    10261 ctgttgtcac tactgccact ttattatttt tagccataga agaaccttgg agatgcccaa
    10321 ttcctacaga gattcctaaa aagaggaacg gcgaaatcac cataaagaag aaactccatg
    10381 actcgacatg tcgaagatag gtttccttga ttacaaccca catatttctc atacttccac
    10441 tcctgattct agtttaaaga tttcatcgat agttggcgct tgttggtcaa atgttgcgat
    10501 atattgatct tgagtcaaga ttgggaagag ttcccttcca gcgctctcat cctccaaaat
    10561 caatttccaa ctgccttgtt tggtcaagct cacctgtttg acatgaggaa gattttccaa
    10621 ttcttccttg cttcgttcac ttgaaacaaa gagacgcgtt ttcccgtatt gattgcggac
    10681 atcctgaact ggtccgtgca agaccacacg gccatctcgg atcatcagaa tatcgtcaca
    10741 aagttcctca acattggtca tgacatggtc agaaaagata atggttgctc cgcgctcttt
    10801 ttcctgaaaa atgacttgtt tgagcaattc tgtattaact gggtccaatc cactaaaagg
    10861 ctcatccaag ataatcaggt ctggttcatg aatcagagta ataatgagct gaatcttctg
    10921 ctgatttcct tttgacagac tcttgatttt atctgtcagc tttcctttca cttccaacct
    10981 cttcatccat tgagggagtt tttctttgac ttctttggca tccatgcctt ttagagtcgc
    11041 caagtagcga acttgttcaa gaactgtcaa tttaggcatg agactgcgtt cttcaggcag
    11101 ataaccaatc cgagcatagg tctcctgacg aatatcctga ccatccagac cgatttctcc
    11161 ctgatattct aggaatttta aaatactatg gaaaatcgtt gtttttccag caccattttt
    11221 tccgactagt cccaaaatac gacctggtcg cgcttgaaag tcaataccaa acaaaacttg
    11281 cttggatcca aaacttttct ctagacttct tacttctagc atctttcacc tccgaaattt
    11341 cttgcactca ttatactcct ttttgatagc ctttacaatg tttttgtcca tttttagaag
    11401 actattgctg tgtaaaatat ggcctggagc acttttatac tcaatgaaaa gcaaagagca
    11461 aactaggaag ctagccgtag gctgctcaaa gtacagcttt gaggttgcag ataaaactga
    11521 cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag tcagtaacta
    11581 tatctacggc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt attagtgata
    11641 aatccattat acagtagcaa acttaattta taccttccgc tcctcaactg tctattttta
    11701 atcctgaatt gttatttgag taactccttt ttcctcataa agttttcttc ctctaaaact
    11761 tctggaaaaa agcgaatagt ttcagacaac atttttataa gaaacaagtt catctgtcat
    11821 ttcaagaagg agtaatcctt tatctactaa tggacggaac ggaattcaac cgcttgtccg
    11881 atatgttttc taaggattat atagtaaaat gaaataaaaa caggacaaat agatcgggac
    11941 agtcaaatcg atttctaaca atgttttaga agtagatgcg tactattcta gtttcaatct
    12001 attatatcta ttatgcacac cactatagga tctaataaaa atcacaacaa gttcattcat
    12061 agatggttac ctcagggaac taagaaaacg actacaaagg aagtcgcatt catcgaaaag
    12121 tggattaaca actatcctaa aaaatgcttg aactacaagt cccccagaga agacttctgg
    12181 atgactaact tgaacttgaa atttagcaat aattaattca ccatctaact atatttagta
    12241 attatttcag aactgattaa tattaaaatt aactaacaat tcaaaggatt catactagcc
    12301 ataaattacg tccatcagag agagattctt actactttta gattttagtc tttctagctt
    12361 cagaatacat ctaaacttta gggaaaatga ctattcgaaa gcgcgaatgc ctcaaaatta
    12421 tctcagataa gctattcgaa acttagaatg cttttaaatt tatggaattg cgattattcg
    12481 aaacctagaa tgcatataac ctttagttga cagacctatt ctaagtctcg aagggctatt
    12541 tactttctat tccttatcaa aaaagactca ttcccccttt ctcctccaaa atatggtata
    12601 gtagaaatat actatctatg aggagtttac atgtcacagg ataaacaaat gaaagctgtt
    12661 tctccccttc tgcagcgagt tatcaatatc tcatcgattg tcggtggggt tgggagtttg
    12721 attttctgta tttgggctta tcaggctggg attttacaat ccaaggaaac cctctctgcc
    12781 tttatccagc aggcaggcat ctggggtcca cctctcttta tctttttaca gattttacag
    12841 actgtcgtcc ctatcattcc aggggccttg acctcggtgg ctggggtctt tatctacggg
    12901 cacatcatcg ggactatcta caactatatc ggcatcgtga ttggctgtgc cattatcttt
    12961 tatctagtgc gcctatacgg agctgccttt gtccagtctg tcgtcagcaa gcgcacctac
    13021 gacaagtaca tcggctggct agataagggc aatcgttttg accgcttctt tatttttatg
    13081 atgatttggc ccattagccc agctgacttt ctctgtatgc tggctgccct gaccaagatg
    13141 agtttcaagc gctacatgac catcatcatt ctgaccaaac cctttaccct cgtggtttat
    13201 acctacggtc tgacctatat tattgacttt ttctggcaaa tgctttgaca cgtaaaaaat
    13261 ccgtttggtt tcccaagtgg atttttaaag cgtagattaa ctatagcttg atactaaata
    13321 tactttggta tggaaatcat gcatattttt cgatagtgag gcgaggactt acctagcctt
    13381 cccgccgtga tagaaacacc tgaaatctaa tggtttcagg tattcggaaa ctttgagcct
    13441 agtgtctcaa agtttaggta tggaatttcg aagaaagtcg ctaccgtccg taatcactta
    13501 aggaaaggct caaaaatatt gttttcaacc acaaaatccg tttggtttcc caagcggatt
    13561 ttgtgcttta ttttgaaact tcttttgcaa gaacaaagtt cccaagtgtg gcagaaccat
    13621 ttcctgcgac tgctggcgtc acgatatagt cacgcacatc tggtactggt aggtaaccat
    13681 taagaagaga tgtaaatttc tcacggacac ggtccagcat atgttgttga gccatgaccc
    13741 ctccaccaaa gacaatcacg tctgggcgga aagtcactgt cgcattaacc gcagcttgag
    13801 cgatatagta ggcttgaaca tcccaaacag ggttgttgag ttcaatattt tccccacgta
    13861 cacctgtacg agcttccaaa cttggaccag ctgcatagcc ttccagacat cccttatgga
    13921 aaggacaaac acccttaaac tctttttcaa tatccattgg gtgtctaaca acataataat
    13981 gacccatttc agggtgaccc acaccaccga taaactcacc acgttggatg acacctgcac
    14041 cgatacctgt accgattgtg tagtaaacca agttttcgat acgaccacca gcattgttac
    14101 gggcaaccac ttcaccgtaa gcagagctgt ttacgtctgt tgtgaagtac attggcacgt
    14161 ttagggcgcg acgaagggca ccaagcaagt ccacatttgc ccagtttggt tttggagtcg
    14221 tcgtgataaa gccataagtt tttgagtttt tgtcaatatc aatcggccca aatgaaccaa
    14281 ctgcaagacc agcaaggtta tcgaattttg agaagaactc aatggtttta tcgattgttt
    14341 cgattggagt tgttgttgga aattgtgttt tttctacaac gttaaagttt tcatcaccga
    14401 cggcacagac aaactttgta ccgcccgctt ccaagcttcc atataatttt gtcatgataa
    14461 acctcttgtt tttattttct ttattatagc atacttcgaa agtctaaatg tctctatttt
    14521 ttagattttc ctctgtaaat cttactatct aataaaaacg aacaaacatg tcatttgttc
    14581 gttttcacat tagagaggat tgattagatt ttcacttcga tcacagcatc ccccttcgaa
    14641 actgaacctg ttgcgactgg agctactgaa gcgtagtcac ctgtatttgt aacgataacc
    14701 attgttgtat catcaagtcc agctgcagcg attttgtttg agtcaaattt tcccagaaca
    14761 tcgccagctt tcaccttatt accttgagaa acttttgctt caaaaccgtc accgttcata
    14821 gatacagtgt caataccaac gtgaatcaaa acttcagcac catttcttgt tttcaaacca
    14881 aaagcgtgcc ctgttggaaa ggcaattgaa acttcagcat cagctggtgc atagaccacg
    14941 ccttggcttg gtttcacagc gataccttgt cccatagctc cacttgagaa gactgggtca
    15001 ttgacatcag caagagcgac aacatcaccg acgataggag ttacaagtgt ttcattttga
    15061 agagctgctg gcgcaacttc ttctttttct tcagccactt cagcttgttt tgcagctgca
    15121 gttgcgtcta cttcatcttc gtaaccaaac atgtaagtaa gagcaaaacc aagggcaaat
    15181 gatacagcta ccataagaag gtattgtgga agttgtccgt tacccacata aagcattgta
    15241 ccagggatga tggtgatacc attaccagta ccagcaagtc caaggataga agccaatcca
    15301 ccaccgattg caccagcaat caatgaaagg aagaatggtt tacggaagcg caagttcacc
    15361 ccgaagatag caggctctgt aatacctagg aaggcagaaa gagcagccgg gaaagcaagt
    15421 gttttcagtt ttggattttt tgttttaaca ccaaccgcaa cagtagcagc accttgagct
    15481 gtcatagcag ctgtgatgat agcgttgaat gggttagcat ggtcagcagc aagtaattgc
    15541 acttcaagca agttgaagat gtggtgcaca cctgacacga cgatcaattg gtgaacccca
    15601 ccaatcaaga aaccaccaag accaaatggc atgctaagaa tcgcttttgt agcaataagg
    15661 atgtagtttt caacaacgtg gaaaactggt ccaatgacaa agagtccaag gatagacatg
    15721 accaaaagtg tcacgaatgg tgttaccaag aggtcaatga catctggaac aaccttgcgg
    15781 acagcttttt caaatttagc tccgacaacc ccgatgatga aggctggaag aacagaacct
    15841 tgcaaaccaa caacagggat gaaaccaaag aagttcatcg ctgttacttc accaccttga
    15901 gcaactgccc aagcgtttgg aagtgagcca gagacaagca tcataccaag aacgatacca
    15961 acggcaggat ttccaccaaa tacacggaag gttgaccaca caaccaaacc tggcaagatg
    16021 atgaaggctg tatctgtcaa gatttgtgtg taagttgcaa agtcacctgg aagtggcatt
    16081 tcaagagcgt tgaaaagacc acgcacaccc atgaagagac ctgtcgctac gataactggg
    16141 atgattggaa cgaaaacatc accgaaagta cggatagcac gttggaacca gttcccttgt
    16201 ttagcaactt ctgctttcat gtcatcctta gatgatgttg gtaagccaag tacaacaact
    16261 tcatcgtaca ttttgttaac tgtacctgta ccaaagataa tttggtattg ccctgagtta
    16321 aagaaagcac cttgaacttt ttccaagttc tcaatcactt ctttattgat tttctcttca
    16381 tctttgacca tgacacgtag acgagtcgca cagtgggcaa cactattgac attttcacgt
    16441 ccgcccaagg catcgatgac tttttttgca atttcctgat tgttcatttg caaaaatctc
    16501 cttatataac attttgttct tgtttgaaag cgattttatt cgcccttacg actattattt
    16561 tatcatgttt taaaaatatg tcaagcgttt tgcagaaaaa atattctatc tacttagtgg
    16621 gcaagcttta gattgattgt ttttgactgt ttcatcagga atttcttgaa aaataagttt
    16681 gaaaccttgg ttaaaaagtc ttttatttca ttatctttca cattttcgta aaaatttgat
    16741 tgatttcctg tatttatttt atgataatcg tttgacagtt ttttctcttt tttaatataa
    16801 aaggcttgca ttttttatcg aaaacagtta ctattaaaag tgataagatt tttggaggaa
    16861 tgaatggaat ggacaactga gcgtcgttac agactttatc aagattggac gcaagaagaa
    16921 attcaacata taaaggaaaa tatggcacaa tctccatggc atactcatta ccatgttgag
    16981 ccaaaaacag gacttctcaa cgacccaaat ggcttttctt actttgatgg caagtggatc
    17041 ctcttttacc agaattttcc ttttggtgca gcccacggtt tgaaatcttg ggcacagcta
    17101 gaaagtgatg atttggttca ctttaaagaa actggaatca aagttttacc agatactcca
    17161 ttagatagcc acggtgccta ctctggttct gccatgcaat ttggcgataa cttattccta
    17221 ttttatacag gaaatgttcg cgatgaaaac tggatccgtc acccatacca gatcggtgct
    17281 ttgatggaca aggagggtaa gattacaaag attgacaaga tcttgattga ccagccagca
    17341 gactctactg accacttccg cgatccacaa atttttaact ttaaaggtca atactatgct
    17401 gtcgtcggtg gacaggactt ggagaaaaaa ggcttcgtcc gtctctacaa ggctgtcaat
    17461 aacgactaca caaactggca agcagttggc gaccttgact ttgctaacga ccgtactgcc
    17521 tacatgatgg aatgtcctaa tctggtcttt gtagaggaac atcctgtcct tctctactgt
    17581 ccacaaggat tggataagaa agttctagac tacgataata tctttccaaa tatgtataag
    17641 attggggctt cctttgaccc taaaaatgcc aaaatggtag atgtgtctca acttcaaaac
    17701 atggattacg gtttcgaagc ctatgcaact caagccttca acgctcctga tgggcgtgct
    17761 ctagcagtta gctggcttgg tttgccagat gtttcttacc catctgaccg ttttgaccac
    17821 caaggaacct tctctttggt caaggaactc actatcaaag acgacaagct ctaccagtat
    17881 ccagtcgctg ctattaagga ccttcgtgct tctgaagaag ccttctcaaa ccgttcccaa
    17941 accaagaaca cttacgaact tgaactcaac ttggaagcta atagccagag cgagattgtc
    18001 ttacttgctg ataaagaagg taagggactt tcaatcaact ttgaccttgt aaacggtcaa
    18061 gtgacagtgg atcgtagcca ggctggagaa cagtatgccc aagaatttgg gacaactcgt
    18121 tcttgcccta tcgagaatca ggctactact gctacaatct tcatcgataa ctctgtcttt
    18181 gaaattttca tcaataaagg agaaaaagta ttttctggtc gtgtcttccc acatgcggac
    18241 caaaatggta tcctgatcaa atctggaaac ccaactggaa tttactatga attagattat
    18301 ggtcacaaaa ctaactgatg tcgccaaact tgcaggcgtc agtcctacta ccgtttctcg
    18361 ggttatcaat aaaaaagggt atctatctga gaaaaccatc caaaaagtca atgaagccat
    18421 gagagaattg ggctataaac ccaacaacct ggctcgtagt ctgcaaggaa aatcagctaa
    18481 gttaatcggc ttgattttcc ccaatatttc caatgttttc tatgcagaat tgattgataa
    18541 attggaacac caactcttca aaaatggtta caagaccatc atctgcaaca gtgaacatga
    18601 ttctgagaag gaacgcgaat acatcgaaat gttggaagcc aatcaggtag acggcatcat
    18661 ttctggtagt cacaacctag gaatcgaaga ctacaatcgt gtgacagcgc cgattatttc
    18721 ctttgaccga aacctatcgc cagacatccc tgttgtctcc tctgacaact atgctggtgg
    18781 ggttcttgct gcccaaacct tggtcaagac aggtgcccag tctatcatca tgattacagg
    18841 gaatgacaat tctaattcgc caaccggact gcgccacgct ggttttgcat ccgtactccc
    18901 aaaagctcct attatcaatg tttccagtga cttttctccc gtcagaaaag aaatggaaat
    18961 caagaatatc ttgacccggg aaaaaccaga tgccattttt gcttcggatg atttgacagc
    19021 tattctggtc attaaaatcg ctcaagaatt gggcatttct gtcccaaaag agctcaaggt
    19081 catcggctat gatgggacct actttatcga aaattactac cctcaattgg ctactatcaa
    19141 gcaacctttg gaagagattg cttgtctcac tattgatctt ctcttgcaaa agattgaagg
    19201 caaggaagtc gccacaactg gttacttctt accagttaca ctattaccag gaaaaagtat
    19261 ttaaacacaa gaaaactcag accgattcgt ctgagttttt atgatcttaa attttcgaga
    19321 tagcgctggg ctgtctctag gttaaaggtt ttatctgaga tgaggcgctc tactagggga
    19381 gcaacttcag attcactagc cccagctagg agagctaggg atttggcctg tagtttcatg
    19441 tgaccttgct ggatgcccgt acttaccaag gctttgaggg ctgcaaaatt ttgagcaaga
    19501 ccgatggaca cgataatctg ggctaattct ctggcagaag gatttcctag tagatcatga
    19561 ctgagagcta cacgtgggtt gaggccgata gagccaccct tagtcgctac aggcatgggc
    19621 agggtcatct caccgaccaa ttcttctctt tcaaggtcca gcgtccagca gctaagacct
    19681 tgatagcgtc catctcgact ggcaaaggca tgggccccag cttcgatggc acgccagtca
    19741 ttaccagtgg caatcaaaat cgcatcaata ccattaaaaa ttcctttatt atgagtagca
    19801 gctcggtaag gatcagcctg cgcaaactga ctagccaatg caattttctc cgcaatctct
    19861 cgtccttgat ccttttggcg gctcaagtag cgaaaggcca tgcgacagct tgcagtcacc
    19921 agagaatcgg tcgcgtagtt ggacaggatt cccatgagac tctgtccctg actgagttct
    19981 tctaagactg gtttcaaggc ttccagcatg gtgttgagca tattggcacc catggcttcc
    20041 tgggtatcaa catgaatata aacaacgaga aagtctggtt caccttttat ctgctcgaca
    20101 tgcaggtcac tcgccccacc cccacgttta acgatagaag gataggcttg attggcaagc
    20161 tccaagagct ccgctttctt gctggcaatc ttctcttgcg ctagtttagg attagcaatt
    20221 tgataaaggg ctacctgccc aatcatctgg cgctgatgga cttgtgcagt aaaaccacct
    20281 gcacgcttga tgattttgct ggcatagctg gccgccgcaa ccacagaggg ttcttctgtc
    20341 acatagggaa cggtgtattc ctgaccgttg acaagtacct ccggaaccag cgaataaggc
    20401 agagaaaaag ttcccaccac attctcactc agctggtctg ccacagtcac actcatctgt
    20461 tcatccttct ccagactagc ttgtctctca ggactaagga gcgcctgagc ttttagcagc
    20521 tcgaggcgct cttggtatga ttttttagaa aatccattcc aacttatctt cattattttt
    20581 caaccttgct ataacggcgt tggtggtcaa gaatttcaac caaggcaaaa tcttgatttt
    20641 catagccagc aaactgggca gagtttgttt catccaagtt tacttcctca aaaaagacct
    20701 tttcatagtc tgcaacggat agggcagttc gttggttgag cttgttcaag cggtctttat
    20761 ccaaataagc ttcatatcct tcaaccaatt caccactgaa gaactcagcc acagctccac
    20821 ttccgtaact ataaagggcg attttatccc cagctttcaa gctatctgta ttttccaaga
    20881 gagacaaaag tccaaggaaa agtgaacctg tgtagatatt ccccaccttt tgactgtaga
    20941 gaatagactg gtcaaaatgc ttttgtaaga ggtctttttt ctcttgaggc aggctcttat
    21001 ccatgatttt tttcaagcct tttagcgcta atttaggata aggcaagtgg aaacaaacag
    21061 ccgcaaaatc atccagagta agctggtagc gtttttgata ttcaagccaa gtcgttttca
    21121 aactatccaa gtattgttgg gtagaataaa caccatttac ataaggagtt gtcgagtaat
    21181 ttggtcgcca gaaatccatg atgtcacggg tctgagctac attgtcatta ttaaaggcca
    21241 tcatgcgtgg attttgtgta atcaacatag ctacacttcc agcaccttga gttggttctc
    21301 ctggagtttc aataccgtat ttggcaatat cactggcaat gaccaagacc ttggactccg
    21361 gagaattttc cacatgcaat ttggcataat gaagggcagc agtcgctcca taacaggctt
    21421 ctttaatctc gaaactacga gcaaagggct ggatgcccag caagccatgc acaaagacgg
    21481 ccgcagcctt actctggtca attcctgact cggtagccac aatgaccatg tcaacttctt
    21541 gtctttcttg ctcagttaaa atagagtcac tagcactggc cgccaaggtc acaatgtcct
    21601 cagttagggg cgcaatactc aattccttga gtaagagtcc tttacttaat ttttcagggt
    21661 caattcccct cgcttctgct aagtcttgta atttcaagac atattgactg gtcgcaaaac
    21721 caatcttatc aataccgatt gtcatattta cctctgtttt atcattcatg taaaaaatcg
    21781 ttctatacta ttttatcaca aatggcagta aaagagagaa aaaagacttg attcaccaaa
    21841 tcaagcctct tattggtcat cattttaaag aatgattagt tgctagagag ttcaccgata
    21901 taagtagctt tataagctcc attcacagtt atcagctcct ggaggatcaa atttcctgag
    21961 taagtccttc ccatctcatc tacaaatttt tgataaaact gactggtcgg aatttctctg
    22021 acatccttat caaatgtctt atcaagtgtt ttactaacct tctcagcaat caattgatgc
    22081 tcttgccatc cactttgaaa ctctgagccc gaactagaaa ccatgactgg gataaacaac
    22141 aaggtcagta gatttacaga caataaggaa agtagtagac ttcctgcaaa actagaatcc
    22201 tagttcatga ttgataatac cagcaatcaa attcattcgt aatccgaatc gtttacgatg
    22261 atttcgatag gttgttgaaa acattttaaa cgtttttact ttggcaaaga tgttctcaac
    22321 cttgcttctc tccttagata gcgcatggtt acaggcttta tcttcagctg ttagcggctt
    22381 gagtttgctg gatttacgtg gagtttgtgc ttgaggatat atcttcatga gcccttgata
    22441 atcactgtca gccaagattt taccagcttg tccgatattt ctgcaactca ttttgaacaa
    22501 cttcatatca tgactatagt tcacagcgat atccaaagaa acaattctcc cttgacttgt
    22561 gacaatcgct tgagccttca tagcgtgaaa tttcttttta ccagaataat tcgctaattg
    22621 ttttttaggg cgattgattt ttacttccgt cacatcaatc attatcgtgt cctcaaagct
    22681 gagaggagtt cttgaaatcg taacaccact ttgaacaaga gttacttcaa cccattggct
    22741 ccgacggatt aagttgcttt cgtgaatacc aaaatcagcc gcaatttctt cataagtgcg
    22801 gtattctcgc acatatagaa agcgttatca atttatttat ctcatttttc agaaaattct
    22861 tttatttctg taaagtctac gatactcgat gtgtttttat ataatgatag agtctgagaa
    22921 tcactgttcc gctagccatt ccaatagaga ttaccaaagc caacatgaca accaaggtcg
    22981 cacttgccag tgctttatta tagtcccctg tcacaaaaaa ggcagttgtt cggtaggaga
    23041 gataacctgg aaccagcggt gccaaaatgg ccaagataaa gaccacagca ggtgtcttat
    23101 aaagaatact taaaatctgg ctgacacaag aaccaataat ggctgcaatg aaggtagcta
    23161 caatgacatt ggtcggttcc ttgagcaaga gatagattag ccagacagtc atgcccaaaa
    23221 tccctccagg taagagcata gaccgttgca cattgagtac gattaaaaaa gtgataatgg
    23281 caagaaaact tgctactgct tgtaataaaa aggttgttag tgtcatatta gttcatcaat
    23341 accaaggcga cagaagttcc tgcccctaaa gcgagggtaa tgagcaggga ttcaaacatc
    23401 ttactcatac cagagtttat gtggttggtc ataatatcac ggaccgcatt ggtcaaggca
    23461 atacctggta caaacggcat gaccgcacca gctataatca aatctgccgt tgaaggaaaa
    23521 cctgtgtagc gagcccaaaa ctgggcaatt atcccaaaga caaaggctcc agcaaaggct
    23581 gtcacaaagg gaattcggat aaatttttcc acatagaggg aaaaggcaaa accaaataag
    23641 gtcgccactc ctgccccaag tgcgtcgtag atatttccgc taaacataac tgaaaagaaa
    23701 ggagcactaa aggtcgcagc cagagttacc tgcaacttag tatagggaag gggtttggct
    23761 tgcaaggccg tcaattgctt aaaggctgtt tctaagtcaa tctgcccccc aactagctga
    23821 cgagaaatct ggttcacatc gcagactttt tcgatgttat aagaagagga ggtcacgcgc
    23881 ttcatgcgcg aaatattggt attttcaata gagaaaaaga tagcggcagg catggcaagg
    23941 acattgcaat ccacaatccc ctgcgaatgc gcgattcgaa tcatggtatc ttctacacga
    24001 tggatttctg agccactttt aaggagaata gtccccgcta gcataatcac atcaatgacg
    24061 gcatttaatt cttttgattc ttccatgctt tcctcctttt atcaactccc tctattctat
    24121 cacaaatccg gactcaaaaa aaatctttgc catgaaatca tgacaaagat tgattactca
    24181 ttttgattat ccatctgctt ttaaggagta gctgaagttg ttttaggttt gtagattgaa
    24241 atcttgactc tagtcttatt taggtctacc ttttcacctg ctctaggact ttgttcaaca
    24301 accatgcctt ctacactacc tgcaggcgct gtcgtcactt ctacaacttc tatattagct
    24361 tccttaatcc caacaatttg aatcaaattg ttcttagtaa actccaagct ggaaccaatg
    24421 taactcggca tggcaacact tgtaactttt ttagctactg tcaagacaat ttgagtaggt
    24481 ttactcacat cataagtcgt tccggcacct ggactttgtt tcataatcgt tcctggttcg
    24541 ctttcgctgg actcttcttc ctctatctta atcaaattct caggaacctt cttctgcttg
    24601 agttctgaga ttacttctgt agagttccgt ccaatatagt tccctaattg aatcgtcgta
    24661 gcttttttag ctactgtcaa aacaatttga gttgccttgc tcaagtcata ggtcgtacct
    24721 tctggtagac tttgcttcag gaccgttcca gcctcactct cattggactc ttcttcctca
    24781 attttaatca aattatctgg aacttttttc tcttttaatt ccgcgataac atcagaagat
    24841 ttccggccga cataattact aatttggaag gattgtttgc ctgatgagac aaccagattg
    24901 attttcgttc cttcttttcg accagttcca gcgccaggat ctgtacggat aatccgccct
    24961 tcttccacct tttcactagc ctctgtcttc tcctcaccaa tctcaaaatt ggcttttttg
    25021 agcgttgcct tggcctctgc aactgtctga cctgccacat ccggaatggc aatggttgca
    25081 ggagttctgg ataagatcca aataagagaa gctgccacca atacaaggct ggccaacaaa
    25141 atcaggtaac gcatcttaaa tctatgtttt ttcggtgctt gtggttggta agtttcctct
    25201 gtcacagcct ggcttgggtt tttgattgat ttgtgttctg tctgcgcttg aaccttagga
    25261 atagatgtca aggtactctg agaaaccttc ggcaaggtct tggtatctgc cttgctcgtt
    25321 tcatcaaaga ttaacttact ttcatttcta cgattgtagg acaagctact agacaagtcc
    25381 acatacatct ctgaaaccga tcggtagcga ttggtcaact ttttagcagt tgccttgata
    25441 ataacatttt ctaaagcctg aggtacagat ggattttctg caataacgga cggcaggggt
    25501 ttctggaaat gctggagggc aatggtcacc gcgctatccc cgtcataagg gatatggcct
    25561 gtcaacatct catagaaaat aatccccatg gcatagatat cactctgcac agtcgcctta
    25621 gaaccacgcg cctgctctgg tgacaagtaa tgaactgagc ccaacatcga gttagtctgg
    25681 gtcagacttg tctctgcaaa ggctacagca atcccaaagt ctgtgacctt ggcagtccca
    25741 tctggtgtca aaaggatatt ttgaggtttc aagtccctgt gaacaattcc tcgagtatgg
    25801 gccaagcgca tagccaagag aatttgtccc atgatacgga cggcttcttc attagaaaga
    25861 ggataatgtt ccttgatata gcgtttgagg tctagtccag caacatactc cattgcaaga
    25921 tactgttgac cgtcttcctc accaatatct gttatccgaa cgatatgagg atggtctaga
    25981 tctgccatag ctctcgcttc acgctgaaaa cgagctacag ctatcgggtc cgtctggtag
    26041 ttggtcctca gaaccttcac tgccacttct tccccatcta agattaagtc tttggctagg
    26101 taaacatccg ccatgcctcc tcgaccaatc tgtttgacaa tccgatagcg tccggcaaaa
    26161 atcttgccga tttggatcat tcttcatcct cctcgttcat agaaacaagg gcaaccgtaa
    26221 tgttgtctaa acctcctgca ttgttagcaa aacgaacaag tgtctccgtt ttatctgcta
    26281 aaggaatatc actggttaca atatcacgaa tctcactgcc tgaaatcatg ttggtcaagc
    26341 cgtcactatt gagcaagaga tagtcacctg actcaaggat aactgtccca aaatcaggct
    26401 gaatttcatc tttttgccca atagactggg tgataatatt tttttgcgga tgagcttctg
    26461 cctcttctgg tgtcaattga ccagccttga gcaattcatt aaccaaggaa tgatcgctcg
    26521 tcaactgatg gtattcttct ccacgaatca agccgatacg cgaatcacca atatgagcat
    26581 agatagcctg attatcaata atagcaagga cttccaaagt agttcccatg cctctgtaag
    26641 cttcatcctg accaagctgg tgaatctttt gattttcaat ttctaggtaa tgggcgaacc
    26701 attcacgcac ttcattgact gtatcgatct gggtatcaac ccaagctaca cccaggtctg
    26761 tgaccgccat ttcactagcg atattccctg cacgatgacc tcccatccca tcagctaaaa
    26821 taatcatggt acgtccagct ctattgacat agtggttgac atagtcttgg ttatttgttc
    26881 gtttctgacc aacatctgtt aataatgaaa tttccatgtg tcagttcctt cctaatccga
    26941 tatcttgcga aattgactga tgaagaatcc atcacttcca tacaattcag gtgtaatgag
    27001 gatacagccg tctttcatga tatccttaca ttcatgttct agttttacct gctcgaactc
    27061 gggatgactc tctaaaaagg ccttaacgac ttgaaaattc tcctctgaga cgatagtgca
    27121 ggtgctataa gttattatac cacctttgcc tagtatttga caaacactac ctaatatttc
    27181 taactgaatt tcctgcaagg acgcgaaatc tgccgtttct ttattgtatt tgatatctgg
    27241 ttttcggcgc aaaagaccga ttcctgaaca aggagcatcc accaaaatct tatcaaagga
    27301 atcctggtca aaaaactcat gcacctttct ggcatccaat ttttgagttt gaacccgatc
    27361 ttcaactccc agacgttggg cattttcttg aattaaatcc aacttgtggt cgtacaagtc
    27421 cagagcagta acctgacctg tcgtaagata agaggctata tgggctgttt tcccacctgg
    27481 agccgcacag gcatcaagca ctcgctcatc accttgtaaa tcaagcgtcg gagcaaccag
    27541 ctgactggac tcgtcttgga tggtaatggc tccatccgca aacaaattat gccctgcaaa
    27601 atgcccctgc tccttaacca gaccagtggt tgctaaaagg gaatcattcg cctccaacaa
    27661 ggcttggatt tcctcttttc gacttaggtc tgttacacga atactggctt tgtttcgcac
    27721 taacaggctt tcaaagatgg cttttgctct ctcctctccg tattcttcct tgagtttggc
    27781 aactagccaa actgggagag aataggcaat ggagtcacgc ttgttttttc gcttgatgct
    27841 agcaatatct ggccagcctt cacgcaagat acggcgaagg agggcattga ccaatttttc
    27901 actgcctttt ttacgaagtt tggccaattc cactgcttca ttgaccacag catgatctgg
    27961 aatcttgtcc aaatagcgga gttggtaggc actcatgaga agaaggacat agagccagct
    28021 gtctaactgg tctctgtctt cgataaagtg ggataggtac cattccagag tcagtttacg
    28081 ggctaccgtt ccatagacca gctcggtcac taagcccttg tctgctgcca aaagttgact
    28141 cccctttaga tgcttattta aggcgatatt tgagtatgct tggttcacaa aaacatcctc
    28201 tagcactgct agagctaaac ttctagccgt ttctacttta gtcaccaaat cgttctccta
    28261 cagtcaatgt acgtccaact ccgttgagga aggaagcaat gtccatctta ggcttaccag
    28321 ctggctgcac ttgtttgagg gatagagccc cttcagccgt tgcgacaatc aattctttct
    28381 tgccgataga gagaatctca cctggatttc cctgaccttc tactggtagg gcttcataaa
    28441 tcttaaagcg gtcgccctta aggaaagcat gggcaacagg ccaggggttc attccacgaa
    28501 tttggttaaa gagttgacga ttggttttgt tccagtccag tttttcttcc tctggcttta
    28561 tatttggaga gaaggtaacc tgactcgtat cctgcggttc aggtttgata tcaccagcaa
    28621 tataggcagg cagagtgtcc aaaagcaaat cacgaccaac tagcgccaat ttttcaaaca
    28681 aggtgccaac attgtcctca tctgtgatcg gaatgctgcg acgagaaatc atatctcctg
    28741 catccatttc cttaaccatt tccatgatgg tcacaccagc ttcctcatcc ccttgaatca
    28801 aggcataatg gataggcgca ccaccacggt gtctaggaag gagggaggca tgaacgttga
    28861 cagcaaagtc catgctatca aggagtttgc ttgggagaaa ctgcccaaaa gcagcagtca
    28921 caattccatc tgctcctagc ttcataagtt cttccatctc tggacttcca gataattttt
    28981 caggttggta gatagatagt cctgcttcct tggcagcctg cttgactggg gtttcttgga
    29041 taactttttt acgaccaaca gcacggtctg gctgggtcac aacggctaga atttcgtaac
    29101 ggtcatctgt caaaagtcct tttaagactg ttgctgaaaa gtcgggggtc cccataaaga
    29161 ttagttttgt catatcttct ccttcttata aaaattgctg cggctcatgg tcaatgctga
    29221 gacggagctc actattttcc cgttcttgag tcaaggccaa gacctggttg agggtcggcc
    29281 ccagctcatc ttctaaacgg tatttaatta aaatctggta atgatagagg ttgtgggtac
    29341 gggcaatcgg ttttggcgtt ggccccagaa tgggactggt ctctgacaag cctgaccgca
    29401 aaatgttcat gacttcatag gcacgtttga aaacctcttc ttctttcttg tgagaaaggg
    29461 taataccaat cgtgaaatag taaggtggat agccgagttg tcgtctgatt cccatttcat
    29521 aggcataaaa gccttcgtaa tcttgatcct tggcaaatcg aatagcatag tgctgcgaat
    29581 tgtaggactg tatcaagact tgacctgctt tttcagcccg acctgctcga cctgccacct
    29641 gagtcaagag ctggaaggtt ctctcagaag aacggaaatc aggcagattc aaggccgtat
    29701 ccgcatttag aactccgact agggtaacat tgggaaaatc caaacccttt gcaatcatct
    29761 gagtaccaag taaaatatcc gcttcccctc gcccaaactg gtcaagcaag gcttggtgac
    29821 tgcctttctt tcgagtcgta tccacatcca tcctcaaaat gcgagcttgg ggaaagagtt
    29881 ctgctagctc atcataagcc ttctgagttc ccgtcccata gtaacgaata ctgcggctct
    29941 tacagttagg acagacctga ggaatatcct tcgagaaacc acaataatgg cagttcatag
    30001 tcttggtatc catatgcaag gtcagagaaa tatcgcagtt gggacaagta tccaccgtcc
    30061 cacactcccg acacatgaca aagctagaat aaccacggcg attgagcatg agaaccacct
    30121 gctctttttt aaccagacgg tcttggatag cctctagcaa aggaggcgta aagtttgacg
    30181 tctcattttg tccgatatag tctcgaaagt caatcacttg aacctcaggg attgtagcca
    30241 aaggattggc acgttgggtt agacgtaagt gttgatagac gcctttgcca gcacgtgccc
    30301 ggctctctaa gctcggcgtt gcagatccaa gtaccagagt tgcttgatta tactgagccc
    30361 gtaaaatagc tacctctctg gcatggtaac ggggattgct gtcctgctta taagccgctt
    30421 catgctcttc atcaataatc atgacaccca gatttttcag aggagcaaag atagcagatc
    30481 tggcaccaac aacaacttgg gcatcgccac gctccacctt gcgccattca tcatactttt
    30541 caccattgga taatcctgag tgaagaatgg ctaccttgtc cccaaaacgt gctataaaac
    30601 gctcggtcat ctgaggagtc aaggaaatct caggtaccag caaaatagct gtcttgccct
    30661 tatccagggc accttggata atctgcaagt aaacctcggt cttcccactt cctgtaatcc
    30721 cttgaagtag aaagggaggt tgagaactgc caatagaact cacaaccgca tcacgcgcct
    30781 gtctttgttc tggatttaac tccaaaggtc tacttgcttc aattccttca aaataagcag
    30841 ccgagcgttg aacttccttt tggactatgg taacagcacc ttgatccaca aagaagttga
    30901 cttgctctcg cgagtaggac tctaacaagc tagccaagga agcgctctct ggatgagaca
    30961 gcagataatc tctcagttcc aactttttct tggcacgtgt agaaatctca acaccttcta
    31021 attgggcatg gtcaacctca taccaagact gggtcttgac cttcttttga tcgactgcct
    31081 gatattccag accaagcagg ccttttctag tcaaacgcat catttcagct tgcttggcaa
    31141 ggtctagtga agaaaaggct agcgaatctt ctgaaccaaa caggcgcact cgttcttcct
    31201 gactcaagcc ttccagagga tagagaatct tgtcatagct agaattcaga aaccctggaa
    31261 gcatggcctt gaggatagag attttgtagg agaagacaga tttgcgtaac tcctcagcca
    31321 gccagagttg ttctggcgtg agaacaggag aaaaatccag cacctctgca atatctttta
    31381 aatcttgctc catctcttct ccatctgatt gggacttcaa accaagaaca atcccttgaa
    31441 tcaggcgatt acccttacca aaaggcacat gaacccgcat cccaacttcc agcattccct
    31501 caaattcctc cggaatcctg taactatagg gctggtccgt ctgcatcaag ggcacatcta
    31561 cgataatctt agctagggcc atcttctcac ctcctccttg tcagcacatt cttgcaatag
    31621 aaaaaataag attgagtccc cccaacctta aattttttca ccatcttctt tttctttagc
    31681 aatttgctct ttgattttct tttcttcttc ttctttgcgg cgtttttctt cttcgatacg
    31741 gcgacgcact gcttcacgtt ttccttctgg atctgggtga attgtaacgt ttcctgattc
    31801 gatttcttct aaagcgcgaa gagttgattt ttcagacttg aaaccttgag ttgctggggc
    31861 acctgcttcc aattcgtggg cacgttttgc ttccaagatt acgagtgaat attttgaagg
    31921 aaccttgtcg agcaaggtat caatagaggg ttttaacatc atttgcttgt acctattttc
    31981 taaattttat cgggtagttg gagattttgg taacatctcc tgatagtgac caatgacacg
    32041 atccacacag aagtgttctg cttcaatcac acatttgaca cgttcagcag ctaggggcac
    32101 ctgatcgttg acaatcgcat aatcatactc acgcatgagg gcaatttctt ccttggcctt
    32161 ttcgattcgt tgggcaatca cttctgcact atctgttcca cgacctacca agcgatcttg
    32221 caattcatcc aaatctggtg gtgtcaggaa gataaagaca gcatctggaa cctttttctt
    32281 gacctgaaga gcaccctgaa cttcaatttc aaggaaaaca tcgattccct tgtccaaggt
    32341 ttcattgaca taggtcagag gagttccata gtagttaccg acatattctg cgtattccaa
    32401 catctgtcct tgacgaatca gctcttcaaa ttcttcacga gtacggaaga aatagtcaac
    32461 accgtccact tctccaggac gttgtgcgcg tgtcgtcatc gatacagaat attgaaattg
    32521 gttttcagaa ctctcaaaaa tctctcttct aaccgttcct tttccaaccc ctgaaggacc
    32581 agaaaaaacg attagtaagc ctcggtctgc cattgtgtct ccttttagtc aatctgtgaa
    32641 ataacatttc tctagaataa tggcaaaaag ccagattatc ctttacagtc tttctatcta
    32701 gtgtaacaaa aaagcagtaa tttttcaact gctctttctt atttatttag cataatctac
    32761 tgcacgaagc tcgcgaatca cggttacctt gatatttcct ggataatcga gattgttttc
    32821 aattttctta cgaactttgt gagccaagat tgtgactttg tcgtccttga tttttcctgg
    32881 attgaccatg atacgaattt cacgtcctgc ttggagggca aagctagttt gcactccttc
    32941 aaagccgtta gcaatttctt ccaaatcatg gagacgcttg atgtagcttt caagagactc
    33001 actacgagca cctggacggg ctgcgctcaa ggcatctgct gcagcgacga taactgctat
    33061 cacgctctca gcttcaacat ctccgtggtg actagcaatc gtattcacca caactggggg
    33121 ttccttgtac ttacgggcca attccatacc gatttcaacg tggctacctt caacctcatg
    33181 gtcaatggct ttcccgatat cgtgaaggaa tccagcacga cgggcaagag ccgcattttc
    33241 accaagttcg ctcgccatga taccagccaa cttagcaacc tcaatcgaat ggcgcaaaac
    33301 attttgtcca tatgaagtac ggaactgcaa acgtcccata atcttcatca agtctggatg
    33361 aaggtttggc gcaccaattt cataggcagc agcctcaccg tattcacgaa tcttattgtc
    33421 aatctcttga cggtttttct caaccaactc ttcgatacga gctggatgta tacgaccatc
    33481 tttgagcaac atttccatag tcatacgggc aatctcacga cgaatcggat caaatcctga
    33541 caaggtcacc acttctggtg tatcgtcgat aatcacatcg acccctgtca aactttcaaa
    33601 ggtacgaatg ttacgacctt cacgaccaat aatgcgtccc ttcatagtat cgtctggcag
    33661 atgaactgtt gagtttgttg actccgctac atattcacca gcgatacgtt gcatagcttg
    33721 aaccaagatg tccttggcca ttttgtcaga acgttccttg acctcttgct cagcttcgcg
    33781 aatgcgactg gcaatctccc tggtcaagtt ttcctctgtc tgagccaaga taatatctcg
    33841 tgcttctgcc tgagacagag caccaatacg ctctagttct gcttcttttt gtctttcgac
    33901 ttcctctaat tgctcttcac gcgcatcaag gtttttcgct ctatcagaaa tactttgttc
    33961 tttttgttca agtgtttgtt ctttactcgt caaattgtcg tccttacggt caaggctagt
    34021 agctctctct gtcaaacgac tttcgatttg tttgagttct tgacgttctg atttgaattc
    34081 agcgtccact tcttcacggt attttctggc ttcttctttg gcctccaata gtgcttcttt
    34141 tttaagagac ttgctttcac gtttggcttc attaacaagt aaatccgctt cacgctcagc
    34201 ttgtccacgt aaattagttg cttcttgttc agcatttaaa agcatcaact ctgcagcttc
    34261 ctgagatgat ttcatcttag ctgagatgct gacatatcca atgactaaac caatgatgac
    34321 ggcaaaaaca gcaatcgcaa gcgacatgat ttccatgttt ttacctcatt ttattgttat
    34381 tccgaatgac atacattctt ttacattcta ccataaaaaa gtgattttca caaacctaaa
    34441 atagaatatg ttttgaggaa attagtctga attttaggat tacacttcct tggtttttta
    34501 aggagataag tcagtgccgc tatcaaaaag ttccaccgaa tttctaccgc tatggatttt
    34561 ctattgttgt gccaaatagt gttccactag attcaaacat tttgctttca agtctttgaa
    34621 aattcacttt aggaaatcac agcttatctt caagatcaaa tttgtcataa attaactttt
    34681 tttatttagt tgtatcaatt tatttcttaa gtattaattt atcctcatcg aaagctt
//
LOCUS       DAYYQN010000016        34371 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYYQN010000016 DAYYQN010000000
VERSION     DAYYQN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.016"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            519..971
                     /locus_tag="ACHBRQ_000715"
     CDS             519..971
                     /locus_tag="ACHBRQ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFM9592424.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            990..2327
                     /locus_tag="ACHBRQ_000716"
     CDS             990..2327
                     /locus_tag="ACHBRQ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9592425.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2406..3290
                     /locus_tag="ACHBRQ_000717"
     CDS             2406..3290
                     /locus_tag="ACHBRQ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9592426.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3290..4123
                     /locus_tag="ACHBRQ_000718"
     CDS             3290..4123
                     /locus_tag="ACHBRQ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9592427.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4141..6234
                     /gene="nanB"
                     /locus_tag="ACHBRQ_000719"
     CDS             4141..6234
                     /gene="nanB"
                     /locus_tag="ACHBRQ_000719"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HFM9592428.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRVESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6246..7348
                     /locus_tag="ACHBRQ_000720"
                     /pseudo
     CDS             6246..7348
                     /locus_tag="ACHBRQ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            7522..8220
                     /locus_tag="ACHBRQ_000721"
     CDS             7522..8220
                     /locus_tag="ACHBRQ_000721"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9592429.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8239..9756
                     /locus_tag="ACHBRQ_000722"
     CDS             8239..9756
                     /locus_tag="ACHBRQ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9592430.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9881..11209
                     /locus_tag="ACHBRQ_000723"
     CDS             9881..11209
                     /locus_tag="ACHBRQ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9592431.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11461..12327
                     /locus_tag="ACHBRQ_000724"
     CDS             11461..12327
                     /locus_tag="ACHBRQ_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9592432.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12343..13182
                     /locus_tag="ACHBRQ_000725"
     CDS             12343..13182
                     /locus_tag="ACHBRQ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9592433.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13455..13907
                     /locus_tag="ACHBRQ_000726"
     CDS             13455..13907
                     /locus_tag="ACHBRQ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFM9592434.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            14004..14348
                     /locus_tag="ACHBRQ_000727"
     CDS             14004..14348
                     /locus_tag="ACHBRQ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592435.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14358..14549
                     /locus_tag="ACHBRQ_000728"
     CDS             14358..14549
                     /locus_tag="ACHBRQ_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592436.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDRHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKKKK"
     gene            14632..15276
                     /locus_tag="ACHBRQ_000729"
     CDS             14632..15276
                     /locus_tag="ACHBRQ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9592437.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15445..16362
                     /locus_tag="ACHBRQ_000730"
     CDS             15445..16362
                     /locus_tag="ACHBRQ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFM9592438.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLDTARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16380..17264
                     /locus_tag="ACHBRQ_000731"
     CDS             16380..17264
                     /locus_tag="ACHBRQ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9592439.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17280..18131)
                     /locus_tag="ACHBRQ_000732"
     CDS             complement(17280..18131)
                     /locus_tag="ACHBRQ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9592440.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18331..20388
                     /gene="pbp2b"
                     /locus_tag="ACHBRQ_000733"
     CDS             18331..20388
                     /gene="pbp2b"
                     /locus_tag="ACHBRQ_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HFM9592441.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTAFSRPMPITAVQALEYSSN
                     AYMVQTALGLMGQTYQPNMFVGTSNLESAMGKLRSTFGEYGLGSATGIDLPDESTGFI
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSVLHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20399..20995
                     /gene="recR"
                     /locus_tag="ACHBRQ_000734"
     CDS             20399..20995
                     /gene="recR"
                     /locus_tag="ACHBRQ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9592442.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPVGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21171..22214
                     /locus_tag="ACHBRQ_000735"
     CDS             21171..22214
                     /locus_tag="ACHBRQ_000735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9592443.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22298..23671
                     /gene="murF"
                     /locus_tag="ACHBRQ_000736"
     CDS             22298..23671
                     /gene="murF"
                     /locus_tag="ACHBRQ_000736"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9592444.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGVELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23694..24269
                     /locus_tag="ACHBRQ_000737"
     CDS             23694..24269
                     /locus_tag="ACHBRQ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HFM9592445.1"
                     /translation="MIALTDTGLTFTKEPFDRERYEDLRSLLSEMLNQVSDLDADEVA
                     ELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEVGYSPTENILKEI
                     EEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQENQEIADLQFFAID
                     QLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24360..25064
                     /locus_tag="ACHBRQ_000738"
     CDS             24360..25064
                     /locus_tag="ACHBRQ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HFM9592446.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25283..26656
                     /gene="ftsA"
                     /locus_tag="ACHBRQ_000739"
     CDS             25283..26656
                     /gene="ftsA"
                     /locus_tag="ACHBRQ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9592447.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26673..27932
                     /gene="ftsZ"
                     /locus_tag="ACHBRQ_000740"
     CDS             26673..27932
                     /gene="ftsZ"
                     /locus_tag="ACHBRQ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9592448.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27937..28608
                     /locus_tag="ACHBRQ_000741"
     CDS             27937..28608
                     /locus_tag="ACHBRQ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9592449.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28618..29157
                     /locus_tag="ACHBRQ_000742"
     CDS             28618..29157
                     /locus_tag="ACHBRQ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9592450.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29157..29420
                     /locus_tag="ACHBRQ_000743"
     CDS             29157..29420
                     /locus_tag="ACHBRQ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9592451.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29417..30202
                     /locus_tag="ACHBRQ_000744"
     CDS             29417..30202
                     /locus_tag="ACHBRQ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9592452.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30211..30999
                     /gene="divIVA"
                     /locus_tag="ACHBRQ_000745"
     CDS             30211..30999
                     /gene="divIVA"
                     /locus_tag="ACHBRQ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HFM9592453.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31347..34139
                     /gene="ileS"
                     /locus_tag="ACHBRQ_000746"
     CDS             31347..34139
                     /gene="ileS"
                     /locus_tag="ACHBRQ_000746"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9592454.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10547 a         6077 c         7504 g        10243 t
ORIGIN      
        1 cttaaaagtt tttggtgaaa cgagatgtag aaaggagatt tagccaaaga gtctattagt
       61 gctagaataa tagattagaa ttattttaga aaaacgaagt gagcagctta taaattcaag
      121 tccccaaata gattcatact agtatctttt gcaaaaaata aagggcgact tccttcatga
      181 atatcaattt catctataag gaaggtagct aattgaacta acttatttat tctgtttgtc
      241 gctagaaaaa tcagacctcc ttgtgaagat tgaggagata ctgaatgaaa atcaaagaag
      301 aaactagcaa gctagcagca gattgcctaa aacaccgctt tgaggttgta gataagactg
      361 acgtatataa tccaaggtga agcgactgtg gtttgaagag attttcaaag agtataggct
      421 agagagtagt gtttttatgt ccttctagta gaaaatgcta gacagaagaa tggggaactt
      481 ggataggaaa aatagattga gaaaggaggt tagaagagat gattattaca aaaattagcc
      541 gtttaggaac ttatgtggga gtcaatccac attttgcaac attaatagat tttctagaaa
      601 aaacaggact agaaaattta acagaaggtt cgattgctat cgacggtaat cgattgtttg
      661 ggaattgctt tacttatcta gcagatggtc aagcagggga tttctttgaa acacaccaaa
      721 aatatttgga tattcattta gttttggaaa acgaagaagc catggctgtt acatcgccgg
      781 aaaatgtaag cgttacccaa gaatatgatg aagagaaaga tattgaatta tacacaggga
      841 aagtggaaca gttggttcat ttgagagctg gcgaatgcct catcactttt ccagaagatt
      901 tacatcaacc caaggttcgt ataaatgatg aacctgtgaa aaaagttgtc tttaaagttg
      961 cgatttctta atgtagaaag agaagaacga tgaaaaaaat gagaaagttt ttatgtctag
     1021 ctggaattgc gctagcggct gttgccttgg tagcttgttc aggaaaaaaa gaagctacaa
     1081 ctagtactga accaccaaca gaattatctg gtgagattac aatgtggcac tcctttactc
     1141 aaggaccccg tttagaaagt attcaaaaat cagcagatgc tttcatgcaa aagcatccaa
     1201 aaacgaaaat caagattgaa acattttctt ggaatgactt ctatactaaa tggactacag
     1261 gtttagcaaa tggaaatgtg ccagatatca gtacagctct tcctaaccaa gtaatggaaa
     1321 tggtcaactc agatgctttg gtgccgctaa atgattctat caagcgtatt ggacaagata
     1381 aatttaacga aactgcctta aatgaagcaa aaatcggaga tgattactac tctgttcctc
     1441 tttattcaca tgcacaagtc atgtgggtta gaacagattt gttaaaagaa cataatattg
     1501 aggttcctaa aacttgggat caactctatg aagcttctaa aaaattgaaa gaagctggag
     1561 tttatggctt gtctgttccg tttggaacaa atgacttaat ggcaacacgt ttcttgaact
     1621 tctacgtacg tagtggtgga ggaagcctct taacaaaaga tcttaaagca gacttgacaa
     1681 gccaacttgc tcaagatggt attaaatact gggttaaatt gtataaagaa atctcacctc
     1741 aagattcttt gaattttaat gtccttcaac aagctacctt gttctatcaa ggaaaaacag
     1801 catttgactt taactctggc ttccatatcg gaggaattaa tgccaacagt cctcaattga
     1861 ttgattcgat tgatgcttat cctattccaa aaatcaaaga gtctgataaa gaccaaggaa
     1921 ttgaaacctc aaacattcca atggttgttt ggaaaaattc aaaacatcca gaagttgcta
     1981 aagcattctt agaagcactt tataatgaag aagactacgt taaattcctt gattcaactc
     2041 cagtaggtat gttgccaact attaagggga ttagcgattc tgcagcctat aaagaaaatg
     2101 aaactcgtaa gaagtttaaa catgctgaag aagtaattac tgaagctgtt aaaaaaggta
     2161 ctgctattgg ttatgaaaat ggaccaagtg tacaagctgg tatgttgact aaccaacaca
     2221 ttattgaaca aatgttccaa gatatcatta caaatggaac agatcctatg aaagcagcaa
     2281 aagaagcaga aaaacaatta aacgatttat ttgaggctgt tcagtagatg taaaagacta
     2341 gaaaataggt gggctagtga gctgaaaagc tctagcccaa ttttgtaaaa gaagggagaa
     2401 ggagaatggt taaagaacgt aatttaactc gctggatatt tgttttgcca gctatgatta
     2461 tcgtaggatt actctttgtt tatccgtttt tctcgagtat tttttatagc tttaccaata
     2521 agcatttgat tatgcctaat tataaatttg ttggtttggc taactataaa gctgtgctat
     2581 cagatcccaa cttctttaat gcgttcttta attcaattaa gtggaccatt ttctcattag
     2641 ttggtcaagt tttagtaggg tttgtattgg ctttagctct tcacagagta cgccacttca
     2701 agaaattata taggacctta ttgattgttc cttgggcatt tcctaccatc gttattgcct
     2761 tctcttggca gtggattcta aacggggttt atggctactt acctaatcta atcgtaaaat
     2821 taggtttaat ggaacataca cctgcatttt tgacagatag tacatgggca ttcctatgtt
     2881 tggtgtttat caacatttgg tttggagcac caatgattat ggttaatgtg ctttcagctt
     2941 tgcaaacagt accagaagaa caatttgagg ctgctaagat agatggtgct tcaagttggc
     3001 aggtgttcaa gtttatcgtc tttccacata ttaaagtggt tgtaggactt ctagttgttt
     3061 tgagaactgt atggatcttt aataactttg acattatcta cctcattact ggaggtggac
     3121 cagccaatgc tacaacgacg cttccaattt ttgcttacaa cctgggttgg ggaactaaat
     3181 tgttgggtcg tgcttcagca gttacagtat tgctctttat cttcttggtg gcgatttgct
     3241 ttatctactt tgctatcatc agtaagtggg aaaaggaggg tagaaaataa tgaagaagaa
     3301 atccagtatt tatttagata ttctctcaca tgtactttta gttggtgcga ccatcgttgc
     3361 aattttccca ttggtatgga ttatcatatc ttctgtcaaa gggaaagggg aattaactca
     3421 gtatccaaca cgattttggc ctgaacagtt tacattagat tatttcactc atgttatcaa
     3481 cgatttgcac ttcattgata acattcgaaa cagtttaatc attgccttgg ctacaaccct
     3541 cattgcgatt attatttctg ctatggcagc ctatggtatt gttcgattct ttcctaaatt
     3601 gggagcaatc atgtcgagac tactcgtcat tacctacatt ttcccaccaa ttttgttagc
     3661 aattccctat tcaattgcca ttgctaaagt tgggttaaca aatagtttat ttggcttgat
     3721 gatggtttat ctatctttta gtgttccata tgcagtttgg ctcttagttg gatttttcca
     3781 aacagttcca attggaattg aagaagcggc tagaattgat ggtgcaaata aatttgttac
     3841 gttttataaa gttgtgctac cgattgtagc accaggtatt gtagcaacag ctatttatac
     3901 atttatcaat gcttggaatg aattcctgta tgccttgatt ttgattaaca atacaggaaa
     3961 gatgacagta gcagtagccc ttcgttcact taatggttca gaaatactag actggggaga
     4021 tatgatggca gcgtctgtta ttgtagttct tccatcaatt attttcttct ctatcatcca
     4081 aaataagatt gcaagtggat tatcagaagg atctgtgaag tagacgaaag aaggaaaaaa
     4141 atgaataaaa gaggtcttta ttcaaaacta ggaatttctg ttgtaggcat tagtctttta
     4201 atgggagtcc ccgctttgat tcatgcgaat gaattaaact atggtcaact gtccatatct
     4261 cctatttttc aaggaggttc atatcaactg aacaataaga gtatagatat cagccctttg
     4321 ttattagata aattgtctgg agatagtcag acagtagtaa tgaaatttaa agcagataaa
     4381 ccaaactctc ttcaagcttt gtttggccta tctaatagta aagcaggctt taaaaataat
     4441 tacttttcaa ttttcatgag agattctggt gagataggtg tagaaataag agacgcccaa
     4501 aagggaataa attatttatt ttctagacca gcttcattat ggggaaagca taaaggacag
     4561 gcagttgaaa atacactagt atttgtatct gattctaaag ataaaacata cacaatgtat
     4621 gttaatggaa tagaagtgtt ctctgaaaca gttgatacat ttttgccaat ttcaaatata
     4681 aatggtatag ataaggcaac actaggagct gttaatcgtg aaggtaagga acattacctc
     4741 gcaaaaggaa gtattgatga aatcagtcta tttaacaaag caattagtga tcaggaagtt
     4801 tcaaatattc ccttgtcaaa tccatttcag ttaattttcc aatcaggaga ttctactcaa
     4861 gctaactatt ttagaatacc gacactatat acattaagta gtggaagagt tctatcaagt
     4921 attgatgcac gttatggtgg gactcatgat tctaaaagta agattaatat tgccacttct
     4981 tatagtgatg ataatgggaa aacgtggagt gagccaattt ttgctatgaa gtttaatgac
     5041 tatgaggagc agttagttta ctggccacga gataataaat taaagaatag tcaaattagt
     5101 ggaagtgctt cattcataga ttcatccatt gttgaagata aaaaatctgg gaaaacgata
     5161 ttactagctg atgttatgcc tgcgggtatt ggaaataata atgcaaataa agccgactca
     5221 ggttttaaag aaataaatgg tcattattat ttaaaactaa agaagaatgg agataacgat
     5281 ttccgttata cagttagaga aaatggtgtc gtttatgatg aaacaactaa taaacctaca
     5341 aattatacta taaatgataa gtatgaagtt ttggagggag gaaagtcttt aacagtcgaa
     5401 caatattcgg ttgattttga tagtggctct ttaagagaaa ggcataatgg aaaacaggtt
     5461 cctatgaatg ttttctacaa agattcgtta tttaaagtga ctcctactaa ttatatagca
     5521 atgacaacta gtcagaatag agtagagagt tgggaacaat ttaagttgtt gcctccgttc
     5581 ttaggagaaa aacataatgg aacttacttg tgtcctggac aaggtttagc attaaaatca
     5641 agtaacagat tgatttttgc aacatatact agtggagaac taacctatct catttctgat
     5701 gatagtggtc aaacatggaa gaaatcctca gcttcaattc cgtttaaaaa tgcaacagca
     5761 gaagcacaaa tggttgaact gagagatggt gtgattagaa cattctttag aaccactaca
     5821 ggtaagatag cttatatgac tagtagagat tctggagaaa catggtcgga agtttcgtat
     5881 attgatggaa ttcaacaaac ttcatatggc acacaagtat ctgcaattaa atactctcaa
     5941 ttaattgatg gaaaagaagc agtcattttg agtacaccaa attctagaag tggccgtaag
     6001 ggaggccaat tagttgtcgg tttggtcaat aaagaagatg atagtattga ttggagatac
     6061 cactatgata ttgatttgcc ttcgtatggt tatgcctatt ctgcgattac agaattgcca
     6121 aatcatcaca taggtgtact gtttgaaaaa tatgattcgt ggtcgagaaa tgaattgcat
     6181 ttaagcaatg tagttcagta tatagatttg gaaattaatg atttaacaaa ataaaggaga
     6241 aaaacatggt taaatacggt gttgttggag cagggtattt tggagctgaa ttggctcgct
     6301 atatgcaaaa gaatgatgga gcagagatta ctcttctcta tgatccagat aatgcagagg
     6361 cgattgcaga agaattggga gcaaaagtag caagttcctt agatgagttg gtttctagcg
     6421 atgaagtaga ttgtgttatc gtcgcaactc caaataatct tcataaggaa ccggttatta
     6481 aggctgcaca gcatggtaaa aatgttttct gtgaaaaacc aattgcgctt tcttatcaag
     6541 attgtcgcga gatggtagat gcgtgtaaag aaaacaatgt aacctttatg gcaggacata
     6601 ttatgaattt ctttaatggt gttcatcatg caaaagaact cattaatcaa ggagttatcg
     6661 gagacgttct atattgtcat acagctcgta atggttggga agaacaacaa ccgtcagtat
     6721 catggaaaaa attcgtgaaa aatcaggtgg tcacttgtat caccacatcc atgaattgga
     6781 ttgcgttcaa ttccttatgg ggggcatgcc tgaaactgta accatgacag gtggaaatgt
     6841 ggcccatgaa ggtgaacatt tcggtgatga agatgatatg atttttgtca atatggaatt
     6901 ttctaataag cgttttgctt tgttagaatg gggttcagct tatcgttggg gtgaacatta
     6961 tgtcttaatc caaggaagca aaggtgccat ccgcttagac ttattcaact gtaaaggaac
     7021 tcttaagcta gatgggcaag aaagctattt cttgattcac gaatcgcaag aagaagatga
     7081 tgatcggact cgtatctatc atagtacaga gatggatgga gcaattgctt atggtaaacc
     7141 aggtaaacgt actccattat ggctatcatc tgtcattgat aaagaaatgc gctatctgca
     7201 tgagattatg gaaggagctc cagtatcaga agaatttgca aaacttttga caggtgaagc
     7261 taccctagaa gcaattgcta ctgcagatgc ttgtacccag tctatgtttg aagatcgcaa
     7321 agtaagattg tcagaaattg taaaataaat tttggtattc tcctatctat aggtcgactt
     7381 gctcctctga aagtactttt agaggagctg tttgactttg ctagtttttg aaactgaaat
     7441 ctattatact acaaactatt gaaagcgttt taattttaag gtataataat ctcatagaaa
     7501 taaagaaaag gaggaaagag gatgccacag attagcaaag aagccttgat tgagcaaatc
     7561 aaagatggaa tcatcgtttc ttgtcaggct cttcctcatg aaccgcttta tacagaagcg
     7621 ggaggggtga ttcccttgct ggtcaaagcg gctgagcaag gtggagcagt cggtatccga
     7681 gcaaacagtg ttcgcgatat caaggaaatt aaggaagtca ctaaacttcc aatcattggg
     7741 attatcaaac gtgattatcc acctcaggaa cccttcatca cggctactat gaaagaagtt
     7801 gatgaattgg cagaactgga catcgaggtg attgctctgg attgtaccaa gcgtgaacgc
     7861 tacgatggtt tggaaattca agagttcatt cgtcaggtta aggagaaata tcctaatcag
     7921 cttttgatgg ctgatactag tatcttcgaa gaagggctag cagctgtaga agcaggaatt
     7981 gactttgtcg gaacaacctt atcaggctac acatcctaca gtccaaaagt agacggtcca
     8041 gattttgaat tgattaagaa actctgtgat gccggtgtag atgtcattgc agaaggaaaa
     8101 attcatacac cagaacaagc caaacaaatc cttgaatatg gagtgcgagg catcgttgtt
     8161 ggtggcgcca ttactagacc aaaagagatt acagaacgct tcgttgctag tcttaaataa
     8221 gatgtgagag ggagttttat gtttaaagtt ttacaaaaag ttggaaaagc ttttatgtta
     8281 cctatagcta tacttcctgc agcaggtcta cttttgggga ttggtggtgc actttcaaac
     8341 ccaaccacga tagcaactta tccaatacta gacaatagta tttttcaatc aatattccaa
     8401 gtaatgagct ctgcaggaga ggttgtattc agtaatttgt cactacttct ctgtgtggga
     8461 ttatgtattg gcttagcgaa acgagataaa ggaaccgctg cgttagcagg agtaactggt
     8521 tacttagtta tgactgcaac gatcaaagct ttggtaaaac tttttatggc agaaggatct
     8581 gcaattgata ctggagttat tggagcatta gttgtcggaa tagttgccgt atatttgcac
     8641 aaccgatata acaatattca attaccttcc gctttaggat tctttggagg ttcacgcttc
     8701 gttcctattg ttacatcgtt ctcttctatc ttgattggct ttgtcttctt tgttatttgg
     8761 ccacctttcc aacaacttct tgtttctaca ggtggatata tttctcaggc gggtccaatt
     8821 ggaacttttc tatatggatt tttaatgaga ctttctggag cagtaggctt acatcatata
     8881 atttacccta tgttttggta tactgaactt ggtggtgttg aaactattgc aggacaaaca
     8941 gtggttggag ctcaaaaaat attttttgct caattagccg atccggccca ttctggatta
     9001 tttacagaag gaacaaggtt ttttgcaggt cgtttctcaa caatgatgtt cggtttaccg
     9061 gctgcctgtt tagcgatgta ccatagtgtt cctaaaaatc gtcgtaaaaa atacgcgggt
     9121 ttgttttttg gagttgcttt aacatctttt attaccggta ttacagaacc aattgaattt
     9181 atgtttctat tcgtcagtcc ggttctatat gttgttcacg cattccttga tggtgttagc
     9241 ttctttattg cagacgtctt aaatatttca ataggaaaca cattttcagg aggtgtaatc
     9301 gatttcactt tatttggaat tttgcagggg aacgctaaga cgaattgggt tcttcagatt
     9361 ccatttggac ttatttggag tgttttgtat tatattattt ttagatggtt cattactcaa
     9421 ttcaacgttc taacgccagg gcgaggagaa gaagtagatt ctaaagaaat ttctgaatcc
     9481 gcagattcaa cttcaaatac tgcagattat ttaaaacagg atagcctaca aattatcaga
     9541 gccttgggtg gatcaaataa tatagaagat gtagatgctt gtgtgacacg tttacgtgta
     9601 gctgtaaaag aagttaatca agttgataaa gcacttttaa aacaaattgg tgcagttgat
     9661 gtcttagaag tgaagggtgg cattcaagca atctatggag caaaagcaat cttatataaa
     9721 aatagtatta atgaaatttt aggtgtagat gattaagtac ttactgactt aataaaaaac
     9781 agaggagagt gatggatgag taggatgaaa tgaaatcgca tacaagaaat aaagaactca
     9841 ttatccaagt tggatacgct tattacatag gagaatacaa atgaaattta gaaaattagc
     9901 ttgtacagta cttgcgggtg ctgcggttct tggtcttgct gcttgtggca attctggcgg
     9961 aagtaaagat gctgccaaat caggtggtga cggtgccaaa acagaaatca cttggtgggc
    10021 attcccagta tttacccaag aaaaaactgg tgacggtgtt ggaacttatg aaaaatcaat
    10081 catcgaagcg tttgaaaaag caaacccaga tataaaagtg aaattggaaa ccatcgactt
    10141 caagtcaggt cctgaaaaaa tcacaacagc catcgaagca ggaacagctc cagacgtact
    10201 ctttgatgca ccaggacgta tcatccaata cggtaaaaac ggtaaattgg ctgagttgaa
    10261 tgacctcttc acagatgaat ttgttaaaga tgtcaacaat gaaaacatcg tacaagcaag
    10321 taaagctgga gacaaggctt atatgtatcc gattagttct gccccattct acatggcaat
    10381 gaacaagaaa atgttagaag atgctggagt agcaaacctt gtaaaagaag gttggacaac
    10441 tgatgatttt gaaaaagtat tgaaagcact taaagacaag ggttacacac caggttcatt
    10501 gttcagttct ggtcaagggg gagaccaagg aacacgtgcc tttatctcta acctttatag
    10561 cggttctgta acagatgaaa aagttagcaa atatacaact gatgatccta aattcgtcaa
    10621 aggtcttgaa aaagcaacta gctggattaa agacaatttg atcaataatg gttcacaatt
    10681 tgacggtggg gcagatatcc aaaactttgc caacggtcaa acatcttaca caatcctttg
    10741 ggcaccagct caaaatggta tccaagctaa acttttagaa gcaagtaagg tagaagtggt
    10801 agaagtacca ttcccatcag acgaaggtaa gccagctctt gagtaccttg taaacgggtt
    10861 tgcagtattc aacaataaag acgacaagaa agtcgctgca tctaagaaat tcatccagtt
    10921 tatcgcagat gacaaggagt ggggacctaa agacgtagtt cgtacaggtg ctttcccagt
    10981 ccgtacttca tttggaaaac tttatgaaga caaacgcatg gaaacaatca gcggctggac
    11041 tcaatactac tcaccatact acaacactat tgatggattt gctgaaatga gaacactttg
    11101 gttcccaatg ttgcaatctg tatcaaatgg tgacgaaaaa ccagcagatg ctttgaaagc
    11161 cttcactgaa aaagcgaacg aaacaatcaa aaaagctatg aaacaatagt ccttagttat
    11221 tctataaaaa gtagtttttt aaagaaccta agagtgtata cccccttttc cctctacaca
    11281 gatagtgtaa gaaaaggggg cttttgttta aaatgtaaga aactgtcacg aaattaaaat
    11341 gaagttctta cataagcgaa tcataaaaaa tttcattttg attttaaaac agttcaagaa
    11401 agtcaaaaaa ttattctatt tgaaagagag gtgccgactg tgaaagtcaa taaaatccgt
    11461 atgcgggaaa cagtgatttc ctacgctttc ctagcaccag tattattctt ctttgtcatc
    11521 tttgtgttgg ctccgatggt gatgggcttc attacaagtt tctttaacta ctcaatgact
    11581 aaatttgagt ttgtaggctt ggataactat atccgtatgt ttaaagatcc tgtctttaca
    11641 aaatctctga ttaacacagt tattttggtt attggatctg taccagttgt tgttctattc
    11701 tcactctttg tagcatctca gacctatcat caaaatgtca ttgccagatc cttctaccgt
    11761 ttcgtcttct tccttcctgt tgtaacgggt agtgttgccg tgacagttgt ttggaaatgg
    11821 atttatgacc cactatcagg gattctaaac tttgtcctta agtccagcca catcatcagc
    11881 caaaacattt cttggttggg agataaaaac tgggcattga tggcgattat gattattctc
    11941 ttgaccactt cagttggtca gcccatcatc ctttatatcg ctgccatggg gaatattgac
    12001 aattcactgg ttgaagcggc gcgtgttgat ggtgcaactg agtttcaagt tttttggaag
    12061 attaaatggc caagccttct tccaacaact ctttatattg caatcatcac aacaattaac
    12121 tcattccagt gtttcgcctt gattcagctt ttgacatctg gtggtccaaa ctactcaaca
    12181 agtaccttga tgtactacct ttacgaaaaa gccttccaat tgacagaata cggctatgcc
    12241 aacacaattg gtgtcttctt ggcagtcatg attgctatcg taagctttgt tcaatttaaa
    12301 gtacttggaa acgacgtaga atactaaaga aaggagacag ctatgcaatc tacagaaaaa
    12361 aaaccattaa cagcctttac tgttatttca acaatcattt tgctcttgtt gactgtgctg
    12421 ttcatctttc cattctactg gattttgaca ggggcattca aatcacaacc tgatacaatt
    12481 gttattcctc ctcagtggtt ccctaaaatg ccaaccatgg aaaacttcca acaactcatg
    12541 gtgcagaacc ctgccttgca atggatgtgg aactcagtat ttatctcatt ggtaaccatg
    12601 ttcttagttt gtgcaacctc atctctagca ggttatgtat tggctaaaaa acgtttctat
    12661 ggtcaacgca ttctatttgc tatctttatc gctgctatgg cgcttccaaa acaagttgtc
    12721 cttgtaccat tggtacgtat cgtcaacttc atgggaatcc acgatactct ctgggcagtt
    12781 atcttgcctt tgattggatg gccattcggt gtcttcctca tgaaacagtt cagtgaaaat
    12841 atccctacag agttgcttga atcagctaaa atcgacggtt gtggtgagat tcgtaccttc
    12901 tggagtgtag ccttcccgat tgtgaaacca gggtttgcag cccttgcaat ctttaccttc
    12961 atcaatactt ggaatgacta cttcatgcaa ttggtaatgt tgacttcacg taacaatttg
    13021 accatctcac ttggggttgc gaccatgcag gctgaaatgg caaccaacta tggtttgatt
    13081 atggcaggag ctgcccttgc tgctgttcca atcgtcacag tcttcctagt cttccaaaaa
    13141 tccttcacac agggtattac tatgggagcg gtcaaaggat aatactctgc gaaaatcgaa
    13201 tgcaaactac gtcagcttca ccttgccata cttaagtatt gcctgcggtt agcttcctag
    13261 tttgttcttc aattttcatt gagtatagga aaatcaatct atcaagatac agaagtatat
    13321 tttatagatt tagagaatat agaggttata agtgtctaca aaatggaggg tatgcagtta
    13381 ctttatgaag ttttgtcaga cgcttatcaa cttaagaatg gttttagtta actatcagaa
    13441 acgaaggaaa gagtatgatt tttgacgatt tgaaaaacat caccttttac aaagggattc
    13501 atcccaattt agacaaggct atcgactacc tctaccaaca tcgtaaagat tcattcgaat
    13561 taggaaagta tgagattgat ggagataaag tctttctagt tgttcaggaa aatgtcctca
    13621 atcaagttga gaataatcaa tttgaacacc ataagaacta tgcagatttg catttgctgg
    13681 tagaaggaca tgaatattcg agctacggtt cacgtatcaa agacgaggca gtagcattcg
    13741 acgaagcgac tgacattggc tttgttcatt gtcatgaaca ctacccactc ttgttgggtt
    13801 atcataattt tgcgattttc ttcccaggtg agccacatca gccaactggc tatgcaggta
    13861 tggaagaaaa ggttcgaaaa tatctcttta aaattttgat tgattaaaaa taggatgaat
    13921 tgtttttttg taaagctttg acaatactct accatgaaat tgatctttgt gaggtagaga
    13981 aatgagaata aaatatttaa aaattggtat cttctaagca tgctgcaaga gctagtttct
    14041 tagatggaca ggggattaca gttgatgaga tgtcctggat aattaatggc attgtgaatg
    14101 cattgattgg tagatacata aaattaggta cttatgcagc taagtatggt attagtatag
    14161 cacgctcgat cttaagtagg gcagctgcaa ctgcagcagc aagagtagga ttactgacca
    14221 agatttctgg atggatttta cgagtagctg tgaatgtagc tgatgtatat ggtaattttg
    14281 ccaacaatat tgctgcagct tgggatgcat atgataaaat tcctaacaat ggtcgtataa
    14341 acttttaaaa tgcgagaatg aaagcacttt gtattttttt attgaatatg ttagcttgga
    14401 cagtgcttgc aatgataatt cgtggagggc tagatggatt tgataggcat acttggagta
    14461 ctattttaat tgcttcgctg ttcggggtat atgattataa gcccatagat aaaaatagaa
    14521 aaaagtccaa aagaaaaaag aaaaaataga tttgttcatg gtagggactt atgaaagctt
    14581 tactgacaaa aaagaaaaca gtttacaaag aaaaatgatg gaggagcaaa catggcacaa
    14641 aaaggagtaa gccttatcaa ggcagcattt gatacagata actttctcat gcgttttagt
    14701 gagaaggtct tggacatcgt gacagccaat cttctttttg tcgtctcttg tttgcccatc
    14761 gtgacgattg gagtggctaa aatcagcctc tacgagacca tgttcgaagt taagaagagc
    14821 agacgggtgc ctgtttttaa aatctatcta agatctttca agcaaaatct gaaactaggt
    14881 cttcagttag gtctgcttga gttgggcatt gtttcattaa gcctcctaga tctttatctc
    14941 ttttggggtc aaacagctct gccctttcaa ttgctgaaag ctatttgtct aggcattctg
    15001 attttcctta cgattgtgat gttggctagc tatccaatcg cggcacgtta tgatttaact
    15061 tggaaagaaa ttcttcaaaa aggattgatg ttggctagtt ttaactttcc ttggttcttc
    15121 ctcatgttag ccattcttgt cctcattgtg atggttcttt atctgtctgc cttcagtcta
    15181 ctcttaggtg gctcagtctt cctgcttttt gggtttggac tattggtctt tatccagact
    15241 ggattgatgg agaaaatttt cgcaaaatac caataggagc cttatttctg aaactacttt
    15301 caaaggctcc aaacgctatt ctataagcga gaaactaaaa tctaagtgtc tataagatat
    15361 tgaaagcctt ttcggtaaag tgtatactaa agaagtaaag aaaaatctgc tttagcagaa
    15421 aataaaaata ataggagaaa atctatgtca gatttgaaaa aatacgaagg tgtcattcca
    15481 gccttctacg catgttatga tgagcaagga gaagtaagcc cagaacgtac gcgtgccttg
    15541 gttcaatact tcattgataa aggtgttcaa ggtctttatg tcaatggttc ttctggtgaa
    15601 tgtatctacc aaagtgttga agaccgcaag ttaatcttgg aagaagttat ggcagttgcc
    15661 aaaggtaaat tgaccattat tgcccatgtt gcttgcaata atactaaaga tagtatggaa
    15721 cttgctcgcc atgctgaaag cttgggagta gatgctattg caacgattcc accaatttat
    15781 ttccgcttgc cagaatactc agttgccaaa tactggaacg atatcagttc tgcagctcca
    15841 aacacagact acgtgattta caacattcct caattggcag gggttgcttt gactccaagc
    15901 ctttacacag aaatgttgaa aaatcctcgt gttatcggtg tgaagaactc ttctatgcca
    15961 gttcaagata tccaaacctt tgtcagcctt ggtggagaag accatatcgt ctttaatggt
    16021 cctgatgagc agttcctagg aggacgcctc atgggggcta gggctggtat cggtggtact
    16081 tatggtgcta tgccagaact cttcttgaaa ctcaatcagt tgattgcgga taaggaccta
    16141 gacacagcgc gtgaattgca gtatgctatc aacgcaatca ttggtaaact cacttctgct
    16201 catggaaata tgtacggtgt catcaaagaa gtcttgaaaa tcaatgaagg cttgaatatt
    16261 ggatctgttc gttcaccatt gacaccagtg actgaagaag atcgtccagt tgtagaagcg
    16321 gctgctgcct tgattcgtga aaccaaggag cgcttcctct aatctaaaag gaggtattta
    16381 tgacatatta cgttgcaatt gatatcggtg gaaccaacat caagtatggt ttggttgatc
    16441 aagaggggca acttcttgaa tcgcatgaaa tgccaactga ggcgcataag ggtggacctc
    16501 atatcttaca aaagaccaaa gatatcgtag ctagttattt agaaaaaggc ccagtagcag
    16561 gtgttgccat atcttctgct gggatggtgg atccggataa gggtgagatt ttctatgctg
    16621 ggccgcaaat ccctaactac gcaggcaccc agttcaaaaa ggaaatcgaa gaaagcttta
    16681 ctattccttg tgagattgaa aatgatgtca actgtgcagg tcttgctgag gcagtatctg
    16741 gttcaggcaa gggagcaagt gtgacacttt gcttgaccat tggaacaggt atcggtggtt
    16801 gcttgattat ggataggaaa gtcttccatg gttttagcaa ttcagcctgt gaagtcgggt
    16861 atatgcatat gcaggatgga gcttttcaag acttggcttc tacaacagct ttagtgaaat
    16921 atgtagctga agcccatgga gaagatgttg atcagtggaa tggccgtaga attttcaaag
    16981 aagccactga aggaaacaaa atctgcatgg aaggtattga ccgtatggtt gactatctag
    17041 gaaaaggtct ggcaaatatt tgctatgttg ccaatccaga agtggttatt cttggtggtg
    17101 gtatcatggg gcaagaggct atcctcaaac ctaagatccg cacagccttg aaagaggctt
    17161 tggtaccaag tttagcagaa aaaacacgat tagaatttgc ccatcaccaa aatacagcag
    17221 ggatgttggg tgcatattat cattttaaga caaaacaatc ctagtttggc tcagccaaac
    17281 taggattttc ttacacgttt ttgtctacga tagccgttga gttttttatt ttcccagtag
    17341 ctattaaaga ttttttcctt gctttcgcga ttgatttcca aaaagtaggc ataaatcaaa
    17401 tcgataaaga agagcatagg aagttgagcg gatattcgtt ggatatagga gggttggctg
    17461 tgggtggcta caagaacagt ctctgtatag gtctggctat ctttattggg aacacttgta
    17521 aagagtacag tctttgcccc catctccttg gcatctaata gactatctaa aatagaagga
    17581 gttgagcctg aaagtgagaa gcccagtact agacaatttt catccatgat gctggttgtc
    17641 caggcaaagc cgtcttggtc tgtcaaagct tcgcagacca cacctagtcg cataaaacgt
    17701 aatttcattt cacgggcgac gaggccagaa ctccctgttc caaagaagta gatacgctca
    17761 gcatcttcga ttagctgggc aattcgttct agttggattt cgtcaatcaa gtcttgtgtt
    17821 tgttccctca tattgctata acttctgagg actcgtttgg tcagtggact gtgcttggag
    17881 acttggttgg cttgattttc tgcctgatgt tggtattgga aaataaattc tcggtagcca
    17941 gtaaagccac actttttagc aaagcgggtc aaagcagctt gagaaatatg taatttttgg
    18001 gtgacttgtt gagaagataa atcatctgta atcgtttcag cttgcaaaaa atagcgagcg
    18061 atttcttgtt ctaggtctgt catttcttca aaatgtgaat caatgatagt tgcgatatct
    18121 ggtttgtcca tagggaaagc tcctttacat gagtcatact ggaagactag atcagggaat
    18181 agtcacactt cattataaca cataatataa ggatagataa ataaaaacgc atctctgttt
    18241 taaaaacgaa aaaatcgaaa aagcttctta cttttccata attttctact caaattgtgg
    18301 tacaattaag agtaagattt taagttagaa atgagactga tttgtatgag aaaatttaac
    18361 agccattcga ttccgattcg gcttaattta ttgttttcaa tcgtcatttt actctttatg
    18421 accattattg gtcgtttgtt gtatatgcag gttttgaaca aggattttta cgaaaaaaag
    18481 ctagcctcag ctagtcagac caaggtcaca accagttctg ctcgtgggga aatttatgat
    18541 gctagtggaa aacctttggt agaaaatacg ttaaaacagg ttgtttcctt tacgcgtagc
    18601 aataaaatga cggctacaga cttaaaagaa acagctaaaa agttactgac ttatgtgagc
    18661 atcagttcgc caaatttgac agaacgccag ctggctgatt actatttggc tgatcctgaa
    18721 atctataaaa aaacagtgga agctctccca agtgagaaac gcttggattc agatggtaat
    18781 cgcctatccg aatcagaact gtataacaat gcggtcgata gtgtcccaac aagtcaacta
    18841 aactatacag aggatgaaaa gaaagaaatc tatcttttta gtcagttaaa tgctgttgga
    18901 aactttgcga caggaaccat tgcgacagat cctctaaatg attctcaggt ggctgttatt
    18961 gcctctattt caaaggagat gcctggcatt agtatttcta cttcttggga tcgaaaggtt
    19021 ttggaaactt ccctttcttc tatagtaggg agtgtatcca gtgaaaaagc tggtctccca
    19081 gcggaagaag cagaagccta tcttaaaaaa ggctattctc taaatgaccg tgttggaacc
    19141 tcctatttgg aaaagcaata tgaagagacc ttacaaggga aacgctcggt aaaagaaatc
    19201 catctggata aatatggcaa tatggaaagt gtggatacaa ttgaggaagg tagtaaggga
    19261 aacaatatca aactgaccat tgatttggcc ttccaagata gcgtggatgc tttgctgaaa
    19321 agttatttca attcagagtt gggaaatggt ggagccaagt attctgaagg tgtctatgca
    19381 gtcgccctta acccaaaaac aggtgctgtt ttgtctatgt cagggattaa acatgacttg
    19441 aaaacgggag agttgacgcc ggattccttg ggaacggtaa ccaatgtctt tgttccaggt
    19501 tcggttgtca aggcggcgac catcagctca ggctgggaaa atggagtctt gtcaggaaat
    19561 cagaccttga cagaccagtc cattgtcttc caaggttcag ctccaattaa ttcttggtat
    19621 actgcctttt ctaggccaat gccgattacg gcagttcagg ctctagagta ttcatccaat
    19681 gcttatatgg tccaaacagc cttaggtctt atggggcaaa cctatcaacc aaatatgttt
    19741 gtcggcacca gcaatctaga gtctgctatg ggtaaattgc gttcaacctt tggcgaatat
    19801 ggcttgggtt ctgcgacagg aattgaccta ccagatgaat ctactggatt tattcccaaa
    19861 gagtatagct ttgctaatta cattactaat gcctttgggc agtttgataa ctatacccca
    19921 atgcaattgg ctcagtatgt ggcaactatt gcaaatgatg gtgttcgtgt ggctcctcgt
    19981 attgttgaag gcatttatgg taataatgat aagggaggac tgggcgactt gattcagcaa
    20041 ctgcaaccga cagagatgaa taaggtcaat atatccgact ccgatatgag tgtcttgcac
    20101 caaggttttt atcaggttgc tcatgggact agcggattga caactggacg tgccttttca
    20161 aatggtgcct tggtatccat tagcggaaaa acgggtacag ccgaaagcta tgtggcagat
    20221 ggtcagcaag caaccaatac caatgcggtg gcctatgccc catctgataa tccccaaatc
    20281 gctgttgcag tggtctttcc tcataatacc aatctaacaa atggtgtagg gccttccatt
    20341 gcgcgtgaca ttatcaatct gtatcaaaaa taccatccga tgaactagaa aggaaattat
    20401 gctttatcca acacctattg ccaagttgat tgacagttat tctaagttac caggtatcgg
    20461 gattaagacg gctacgcgtc tggcctttta tacgattggg atgtctgctg atgatgtcaa
    20521 tgaatttgca aaaaatctcc tttctgctaa gagagaattg acatattgtt ctatttgtgg
    20581 acgtttgaca gacgacgatc cttgttctat ctgtactgat ccgactcgtg accagacaac
    20641 aattttagtt cttgaggata gtagagatgt ggcagccatg gaaaatatcc aagaatacca
    20701 tggactctat catgtcctgc atggcctcat ttctcctatg aatggtatca gtccggacga
    20761 tatcaatctc aagagcctta tgactcgtct tatggatagt gaggtttcag aagtgattgt
    20821 ggcgactaat gctacagcgg atggtgaagc gacttccatg tatctttcac gtttgctcaa
    20881 gccggttggt atcaaggtta cgcgtctagc acgaggtctc gctgtgggag cggacattga
    20941 gtatgcggac gaagtgacac tcttacgagc gattgaaaat cggacagagt tgtaagtgta
    21001 ggcaaattta cgaactccct tcatttataa aaaatcaaag aggctgaaaa tcgttcctat
    21061 cggcctcttt ttgtatagtg tgatgagtag gatcgggttc aagttttaaa aaaccaagca
    21121 aatatgatat actaaagagc gagtattcta gtagaattag gacaaataat atgaaacaaa
    21181 cgattattct tttatatggt ggacggagtg cggaacgcga agtctctgtc ctttcagctg
    21241 agagtgtcat gcgtgcggtc aattacgacc gtttcacagt caagactttc tttatcagtc
    21301 agtcaggtga ctttatcaaa acacaggaat ttagtcatgc tccggggcaa gaagaccgtc
    21361 tcatgaccaa tgaaaccatt gattgggata agaaagttgc accaagtgct atctacgaag
    21421 aaggtgcagt ggtctttcca gtccttcacg ggccaatggg agaagatggc tctgttcaag
    21481 gattcttgga agttttgaaa atgccttacg ttggttgcaa cattttgtca tcaagtcttg
    21541 ccatggataa aatcacgact aagcgtgttc tggaatctgc tggtattgcc caagttcctt
    21601 atgtggctat cgttgaaggc gatgatgtga ctgctaaaat cgctgaagtg gaagaaaaat
    21661 tggcttatcc agtcttcact aagccgtcaa acatggggtc tagtgtcggt atttctaagt
    21721 ctgaaaacca agaagaactc cgtcaagcct taaaacttgc cttccgatat gacagccgtg
    21781 tcttggttga gcaaggagtg aatgcccgtg aaattgaggt tggcctcttg ggtaactacg
    21841 atgtcaagag cacgctacct ggagaagttg tcaaggacgt tgccttttat gactacgatg
    21901 ccaagtatat tgataacaag attactatgg atattcctgc caaaatcagt gatgatgtgg
    21961 tggctgtcat gcgtcaaaat gcagaaacag ccttccgtgc cattggtggc cttggtctat
    22021 ctcgttgcga tttcttctat acagataagg gagagatttt tctcaacgag ctcaatacca
    22081 tgccaggttt cacccagtgg tctatgtacc cactactttg ggacaatatg gggatcagct
    22141 acccagaact aatcgagcgt ttggttgacc ttgccaagga aagttttgac aagcgcgaag
    22201 cgcatttgat ataaaatgaa agagagggta gaagccagaa ccatcactgc aaggtgacta
    22261 gagttctcgg acttcagccc tttttaaagg agtagaaatg aaattaacaa tccatgaaat
    22321 tgcccaagtt gttggagcca aaaatgatat cagtatcttt gaggacaccc agttagaaaa
    22381 agctgagttt gatagtcgtt tgattggaac tggagattta tttgtgccac ttaaaggtgc
    22441 gcgtgacggt catgacttta tcgaaagagc ttttgaaaat ggtgcggcag caaccctgtc
    22501 tgagaaagag gttgcaaatc atccctacat tctagtagat gatgttttga ctgcctttca
    22561 acaactagct gcctactatc ttgaaaaaac agctgttgat gtctttgcgg ttacgggttc
    22621 aaatggcaag acaacgacta aggatatgtt ggcgcattta ctgtcaacga cctacaagac
    22681 ctacaaaaca caaggcaact acaataacga gattggcctt ccctacacag ttctacacat
    22741 gcctgaagga acagaaaagt tggttttgga gatggggcag gaccacttgg gcgatattca
    22801 tctcttgtct gaattggctc gtccaaaaac agccatcgtg accttggttg gagaagctca
    22861 tttagccttt ttcaaagatc gttctgagat tgctaaagga aaaatgcaaa ttgcagacgg
    22921 aatggcttca ggttccttgc ttttagcgcc ggctgaccct atcgtagagg actatttgcc
    22981 aactgataaa aaggtggttc gttttgggca aggggtagag ctggaaatta ctgacttggt
    23041 tgagcgcaaa gatagtctga ccttcaaggc caatttctta gagcaagtcc ttgatttgcc
    23101 agtaactggc aagtacaatg cgacaaatgc tatgattgca tcctatgttg ccttgcaaga
    23161 aggagtttca gaggagcaaa ttcgtttggc cttccaagat cttgaattga cgcgtaaccg
    23221 taccgagtgg aagaaagcag ccaatggagc agatatcctg tcagatgttt acaatgccaa
    23281 tccaactgct atgaaactga ttttagagac tttctctgcc attccagcca atgaaggtgg
    23341 caagaaaatt gcagttttgg cggatatgaa ggagcttggt gaccagtctg ttcaacttca
    23401 taatcagatg attttgagcc tctccccaga tgtgcttgat atcgttattt tctatggaga
    23461 agacattgct gaattagccc aattggccag tcaaatgttc ccaattggcc acgtttacta
    23521 cttcaagaaa acagaagacc aagaccaatt tgaagactta gtcaagcagg tcaaggaaag
    23581 cctcagtgcc aatgatcaaa tcttgctcaa gggctctaac tctatgaacc tagccatgtt
    23641 ggtagaaagt ttagaaaatg aaaccaagtg attttgttaa gtatctgtaa agaatgattg
    23701 cccttacaga tactggctta acctttacaa aagagccttt tgaccgtgag cgctacgaag
    23761 acttgcgaag tctgttatct gaaatgttga atcaggtatc agaccttgat gcagacgaag
    23821 tggcagaact cttgaaaccg acctccgctt atgcgactcc cttaatggac gtccgtgctt
    23881 ggattgttga ggatgagaag atttgtctgg ttaggggaca aggagaggat agttgggctt
    23941 tgccaggtgg ctttggtgaa gtcggctatt ctccaactga aaatattctc aaggaaattg
    24001 aagaagaaac cggttttaaa gccaaagttg aaagactgct agctgttttt gataccaatc
    24061 gtttccaact acagagcaaa caatatgcaa agtttgtttt tgaatgtaag cttctcgatg
    24121 gacaattcca agaaaatcaa gaaatcgctg accttcaatt ttttgcaatt gaccaactgc
    24181 cgaatttatc tgaaaaacgc attaccaagg agcaaatcga gattctttgg caggtttatc
    24241 aaggtcagag ggagcaatat cttgactaag aagatgatta tcgtatttct aaattcattt
    24301 ttcttaagta gcatggtata ataaaatgca ggaaaatttt gaatcatgag gaagactaga
    24361 tgaatttatg ggatattttc tttacgactc aggcaaccga gccgcccaaa tttgaccttt
    24421 tttggtatgt tagcctattt acgctcttag ccttaacctt ttatacagcc catcgctatc
    24481 gtgaaaagaa ggtttaccaa cgatttttcc aaatcttgca gactgttcag ttaatccttc
    24541 tttatggttg gtactgggtc aatcatatgc cactgtcaga aagcctaccc ttttaccatt
    24601 gccgtatggc tatgtttgtg gtactcttgc ttcctggtca gtccaaatat aaacaatact
    24661 ttgcattatt gggaacattt gggacattag cagcctttgt ttatccagtg ccagatgctt
    24721 acccttttcc acatatcacc attctatcct ttatctttgg tcatttagca ctcttgggga
    24781 actctctagt ttatctattg agacagtata atgcgcgatt gctggatgtg aagggaattt
    24841 ttctcatgac ctttgcccta aatgccttga tttttgtggt caatttggtg acaggtggcg
    24901 attacggatt tttgacaaaa ccgccattgg ttggggatca cggtctagta gctaattatt
    24961 tacttgtttc aattgtgctg gtagctacta tcagtttgac taagaaaatc ttagaattct
    25021 ttttagctca agaagcagaa aaaatgattg caaaggaagc ttaacacaga gctttctttt
    25081 ttgctcttag agagttttta caagcagctt ataaaataag aatttctgaa tagacaaact
    25141 caaaaaatgg ctgggaaatt taggaaaaaa gcaagcacga ttaaattttt tgtgttataa
    25201 tattttgtga atagctatgc ctatgtttag ctatggaata atacgaagtg cgaaacttgg
    25261 aagatagaga ggaagcgatg taatggctag agaaggcttt tttacaggtc tagatattgg
    25321 aacaagctct gtcaaggtgc ttgtggccga gcagagaaat ggtgaattaa atgtaattgg
    25381 cgtgagtaat gccaaaagta aaggtgtaaa ggatggaatt attgttgata ttgatgcagc
    25441 agcaactgct atcaagtcag ccatttccca agcggaagaa aaggcaggca tttcgattaa
    25501 atcagtaaat gtcggcttgc ctggtaatct tttgcaggta gaaccaactc aggggatgat
    25561 tccagtaaca tctgatacta aggaaattac ggatcaagat gttgaaaatg ttgtcaaatc
    25621 agctttgaca aagagtatga cacctgaccg tgaagtcatt acctttattc ctgaagaatt
    25681 tattgtggat ggtttccaag ggattcgtga cccacgtggc atgatggggg ttcgccttga
    25741 aatgcgtggt ttgctttata caggacctcg tactatcttg cacaatttgc gtaagacggt
    25801 tgagcgtgca ggtgttcagg ttgaaaatgt tatcatttca ccactagcaa tggttcagtc
    25861 tgttttgaac gaaggggaac gtgaatttgg tgctacagtg attgatatgg gggcaggtca
    25921 aacgactgtc gctacaatcc gtaatcaaga actccagttc acacatattc tccaagaagg
    25981 tggagattat gtaactaaag atatctccaa ggttttgaaa acctctcgca aattagcgga
    26041 aggcttgaaa ctgaattacg gggaagccta tccgcctctt gcaagcaaag aaaccttcca
    26101 agtagaggtt attggagaag tagaagcagt cgaagtgacg gaagcctact tgtcagaaat
    26161 tatttctgca cgaatcaagc acatccttga acaaatcaag caagaattag atagaaggcg
    26221 tctattggac ctccctggtg gtattgtctt aatcggtggg aatgccattt taccaggtat
    26281 ggttgagctt gctcaggaag tctttggcgt ccgtgtcaag ctttatgttc caaatcaagt
    26341 tggtatccgt aatccagcct ttgcgcatgt gattagttta tcagaatttg cgggtcaatt
    26401 aacagaagtt aatcttttgg ctcagggagc gataaaaggt gagaatgact taagtcatca
    26461 gccaattagt tttggtggga tgctgcaaaa aacagctcag tttgtacaat caacgcctgt
    26521 tcaaccagct cctgctccag aagtagagcc ggtggcgcct acagaaccaa tggcggattt
    26581 ccaacaagct tcacaaaata aaccgaaatt agcagatcgt ttccgtggat tgatcggaag
    26641 catgtttgac gaataaagag gaaaaataaa ttatgacatt ttcatttgat acagctgctg
    26701 ctcaaggggc agtgattaaa gtaattggtg tcggtggagg tggtggcaat gccatcaacc
    26761 gtatggtcga cgaaggtgtt acaggcgtag aatttatcgc agcaaacaca gatgtacaag
    26821 cattgagtag tacaaaagct gagactgtta ttcagttggg acctaaattg actcgtggtt
    26881 tgggtgcagg aggtcaacct gaggttggtc gtaaagccgc tgaagaaagc gaagaaacac
    26941 tgacggaagc tattagtggt gccgatatgg tcttcatcac tgctggtatg ggaggaggct
    27001 ctggaactgg agctgctcct gttattgctc gtatcgccaa agatttaggt gcgcttacag
    27061 ttggtgttgt aacacgtccc tttggttttg aaggaagtaa gcgtggacaa tttgctgtag
    27121 aaggaatcaa tcaacttcgt gagcatgtag acactctatt gattatctca aacaacaatt
    27181 tgcttgaaat tgttgataag aaaacaccgc ttttggaggc tcttagcgaa gcggataacg
    27241 ttcttcgtca aggtgttcaa gggattaccg atttgattac caatccagga ttgattaacc
    27301 ttgactttgc cgatgtgaaa acggtaatgg caaacaaagg gaatgctctt atgggtattg
    27361 gtatcggtag tggagaagaa cgtgtggtag aagcggcacg taaggcaatc tattcaccac
    27421 ttcttgaaac aactattgac ggtgctgagg atgttatcgt caacgttact ggtggtcttg
    27481 acttaacctt gattgaggca gaagaggctt cacaaattgt gaaccaggca gcaggtcaag
    27541 gagtgaacat ctggctcggt acttcaattg atgaaagtat gcgtgatgaa attcgtgtaa
    27601 cagttgtcgc aacgggtgtt cgtcaagacc gcgtagaaaa ggttgtggct ccacaagcta
    27661 gatctgctac taactaccgt gagacagtga aaccagctca ttcacatggc tttgatcgtc
    27721 attttgatat ggcagaaaca gttgaattgc caaaacaaaa tccacgtcgt ttggaaccaa
    27781 ctcaggcatc tgcttttggt gattgggatc ttcgccgtga atcgattgtt cgtacaacag
    27841 attcagtcgt ttctccagtc gagcgctttg aagccccaat ttcacaagat gaagatgaat
    27901 tggatacacc tccatttttc aaaaatcgtt aagtaaatga atgtaaaaga aaatacagaa
    27961 cttgtttttc gagaagttgc agaggctagt ctgagtgctc atcgagagag tggttcggtc
    28021 tctgtcattg cagttaccaa gtatgtagat gtaccgacag cggaagcctt gcttccgcta
    28081 ggtgttcatc atatcggtga aaatcgtgta gataagtttc tggaaaaata tgaagcttta
    28141 aaagatcgag atgtgacttg gcatttgatt ggtaccttgc aaagacgtaa ggtgaaagat
    28201 gtcattcaat acgttgatta tttccatgca ttggactcag taaagctagc aggggaaatt
    28261 caaaaaagaa gtgaccgagt catcaagtgt ttccttcaag taaatatttc taaagaagaa
    28321 agcaaacacg gtttttcgag agaggaactg ctggaaatct tgccagagtt agccagacta
    28381 gataagattg aatatgttgg tttaatgacg atggcacctt ttgaggctag cagtgagcag
    28441 ttgaaagaga ttttcaaggc ggcccaagat ttacaaagag aaattcaaga gaaacaaatt
    28501 ccaaatatgc ctatgaccga gttaagtatg ggaatgagtc gtgattataa agaagcgatt
    28561 caattcggtt ccacttttgt tcgtataggt acatcatttt ttaagtagga gagaaccatg
    28621 tctttaaaag atagattcga tagatttata gattatttta cggaggatga ggattcaagt
    28681 ctcccttatg aaaaaagaga tgagcctgtg tttactccag taaattcttc acaggaaccg
    28741 gctctcccaa tgaatcaacc ttcacagtcg gctggtacaa aagagaacaa tatcaccaga
    28801 cttcatgcaa gacaacagga attggcaaat cagagtcagc gtgcaacgga taaggtcatt
    28861 atagatgttc gttatcctag aaaatatgag gatgcaacag aaattgttga tttattggca
    28921 ggaaacgaaa gtatcttgat tgattttcag tatatgacag aggtgcaggc tcgtcgttgt
    28981 ttggactatt tggatggagc ttgtcatgtt ttagctggaa atttgaaaaa ggtagcttct
    29041 accatgtatt tgttgacacc agttaatgtt attgtaaatg ttgaagatat ccgtttacca
    29101 gatgaagatc aacagggtga gttcggtttt gatatgaagc gaaatagagt acgataatga
    29161 tttttttaat tcgtatgatt tataatgcag tggatattta ctccctgatt ttggtagcct
    29221 tcgctgtcat gtcttggttt ccaggtgcct acgaatccag tttaggtcgt tggattgtag
    29281 cgttggtgaa accagtactt gctcccttgc aacgcctgcc tttacagata gcgggtcttg
    29341 atttatctgt ttgggtcgcg attgttttgg ttcgattttt aggagaaaac ctagtgcgtt
    29401 ttctggcgat gataggatga ataaagggat ttatcagcat ttctccatag aagatcgtcc
    29461 atttcttgac aagggaatgg aatggataaa gaaggtagaa gatagctatg ctcctttttt
    29521 aactcctttt atcaatcctc atcaggagaa gctattaaag attttggcca aaacctatgg
    29581 tcttgcttgt agcagtagtg gggaattcgt ctcgagtgag tatgttcgag ttttattata
    29641 cccagattat ttccaaccag agttttcaga ttttgaaata tctctccaag aaattgtgta
    29701 ttccaataaa tttgaacatt taacgcatgc taagatttta gggacagtca tcaatcaatt
    29761 agggattgaa cggaaacttt ttggagatat cctagtagat gaagaacggg cgcagattat
    29821 gattaatcag cagtttcttc ttctctttca agatggacta aagaaaattg gtcgtatacc
    29881 tgtttcgctg gaggaacgtc ctttcaccga gaaaatagat aagctagaac agtatcgaga
    29941 actggattta tctgtgtcta gttttcgatt agatgttctt ttatcaaatg ttttgaaact
    30001 atctaggaat caagcaaacc agttgattga aaagaaactt gtccaagtaa attatcatgt
    30061 ggtagacaaa tcagattaca ctgttcaagt tggagacttg attagtgtga gaaaatttgg
    30121 tcgcttgaga ttacttcaag ataagggaca aacgaaaaaa gagaagaaaa aaataaccgt
    30181 ccagttatta ttaagtaagt gaggaataga atgccaatta catcattaga aataaaggac
    30241 aagacttttg gaactcgatt cagaggtttt gatccagaag aagtcgatga atttttagat
    30301 attgtggttc gtgattacga agatcttgtg cgtgcgaatc atgataaaaa tttgcgtatt
    30361 aagagtttag aagagcgttt gtcttacttt gatgaaataa aagattcatt gagccagtct
    30421 gtattgattg ctcaggatac agctgagaga gtgaaacagg cggcgcatga acgttcaaac
    30481 aatatcattc atcaagcaga gcaagatgcg caacgcttgt tggaagaagc taaatataag
    30541 gcaaacgaga ttcttcgtca agcaactgat aatgctaaga aagtcgctgt tgaaacagaa
    30601 gaattgaaga acaagagccg tgtcttccac caacgtctca aatctacaat tgagagtcag
    30661 ttggctattg ttgaatcttc agattgggaa gatattctcc gtccaacagc tacttatctt
    30721 caaactagtg atgaagcctt taaagaagtg gttagcgaag tacttggaga accgattcca
    30781 gctccaattg aagaagaacc aattgatatg acacgtcagt tctctcaagc agaaatggaa
    30841 gaattacaag ctcgtattga ggtagccgat aaagaattgt ctgaatttga agctcagatt
    30901 aaacaggaag tggaaactcc aactcctgta gtgagtcctc aagttgaaga agagcctctg
    30961 ctcatccagt tggcccaatg tatgaagaac cagaagtagc tccagtgcat ccgacaggtc
    31021 caacaccagc tacagaaact gttgattcag caccaggatt tgaagcaccg caagaatccg
    31081 ttacaatttt ataagaaacg atgtgagaac aatatcttat ccttatattt ccagcgagca
    31141 ggagatggtg tgagtcctgc attccctaac gataagatta tcctctcaaa aactcaagtc
    31201 tgaagctagt aagatttgac gtttcccacg ttacgggata agagggagaa agactaaatc
    31261 tttttccgaa taaaggtggt accacgattt tcgtcctttt tggaagtcgt ggtttttaat
    31321 ttgttattat ttataaagga gataccatga aactcaaaga tacccttaat cttgggaaaa
    31381 cagctttccc aatgcgtgca ggccttccga ccaaagagcc agtttggcaa aaggaatggg
    31441 aagatgcaaa actttaccaa cgtcgtcaag aactaaatga agggaaaccg cattttgtct
    31501 tgcatgatgg ccctccatac gcaaacggaa atatccacgt tggacatgcc atgaatcata
    31561 tctcaaaaga tatcatcatt cgttctaagt ctatgtcagg ttttaacgca ccttatatcc
    31621 caggttggga tactcatggt ctgccaatcg agcaagtttt ggcaaagcaa ggtgtgaagc
    31681 gcaaagaaat ggacttggtt gagtacttga aactctgtcg tgagtacgct ctttctcaag
    31741 tttataaaca acgtgatgat ttcaaacgct tggggatgtc aggtgactgg gaaaatttat
    31801 atgtgacttt aactccagac tatgaagcag cccaaatccg tgtctttggt gagatggcta
    31861 ataagggtta tatctaccgt ggtgccaagc cagtttactg gtcatggtca tctgagtcag
    31921 cccttgccga agcggaaatt gaataccatg atttggtttc gacttcactt tattatgcca
    31981 ataaggtaaa agatggcaaa ggtatcctag atacagatac ttatatcgta gtctggacaa
    32041 caactccatt taccatcaca gcttctcgtg gtttgactgt tggtgcagat attgattacg
    32101 ttttggttca acctgctagt gaaactcgta agtttgtcgt tgctgcagaa ttgttgacta
    32161 gcttgtctga gaaatttggc tgggctgatg ttcaagtgtt ggcaacttac cgtggtcaag
    32221 aactcaacca catcgtaaca gaacacccat gggatacagc tgtagatgag ttggtaatcc
    32281 ttggtgatca cgttaccact gactctggta caggtatcgt ccatacagcc cctggttttg
    32341 gtgaggacga ctacaatgtt ggtattgcta atggtcttga agtcgcagtg actgttgacg
    32401 aacgcggtat catgatggcg aatgctggtc ctgagtttga aggtcagttc tacgacaagg
    32461 ttgtaccaac tgttattgaa aaacttggta acctccttct agcccaagaa gaaatctctc
    32521 actcataccc atttgactgg cgtactaaga aaccaatcat ctggcgtgca gttccacaat
    32581 ggtttgcctc agtttcaaaa ttccgccaag aaatcttgga tgctattgat aaagttaaat
    32641 tccatacaga atggggtaaa gtacgtctat ataatatgat tcgtgaccgt ggtgactggg
    32701 ttatctctcg tcaacgtgct tggggtgttc cacttccaat cttctatgca gaagacggta
    32761 cagctatcat gacagctgag actattgaac atgtggcgca actctttgaa gtacatggtt
    32821 caagcatttg gtgggaacgt gatgccaaag acctcttacc agaaggattt acacatccag
    32881 gttctccaaa tggggagttc aagaaagaaa cagatattat ggatgtatgg tttgactcag
    32941 gttcatcatg gaatggtgtt ttggtaaatc gtccaaatct gacttatcca gctgaccttt
    33001 accttgaagg ttctgaccaa taccgtggtt ggttcaactc atcactcatt acatctgttg
    33061 ccaaccatgg cgtagcacct tacaaacaaa tcttgtcaca aggttttacc cttgatggta
    33121 aaggtgagaa gatgtctaaa tcacttggaa ataccattgc tccaagtgat gttgaaaaac
    33181 aatttggtgc agaaatcttg cgtctctggg taacaagtgt tgactcaagc aatgacgtgc
    33241 gtatctctat ggatattttg agccaagttt ctgaaactta ccgtaagatt cgtaacactc
    33301 ttcgtttctt gattgccaat acatctgact ttaacccagc tcaagatgta gtcgcttacg
    33361 atgaacttcg ttcagttgat aagtacatga ctattcgctt taaccagctt gtcaagacca
    33421 ttcgtgatgc ctatgcggac tttgaattct tgacaatcta caaggccttg gtgaacttta
    33481 tcaacgttga cttgtcagcc ttctaccttg attttgccaa agatgttgtt tacattgaag
    33541 gtgccaaatc attggaacgc cgtcaaatgc agactgtttt ctatgacatt cttgtcaaaa
    33601 tcaccaaact cttgacacca atccttcctc acactgcgga agaaatctgg tcatatcttg
    33661 agtttgaagc tgaagacttc gttcaattgt cagaattgcc agaagctcaa acttttgcta
    33721 atcaagaaga agtcttggat acatgggcag ccttcatgga cttccgtgga caagctcaaa
    33781 aagccttgga agaagctcgt aatgcaaaag ttatcgggaa atcgcttgaa gcacacttga
    33841 cagtttatcc aaatgaagtt gtgaaaactc tactcgaagc agtaaacagc aatgtggctc
    33901 aacttttgat cgtgtcagac ttgaccatcg cagaagggcc agctccagaa gctgccctta
    33961 gcttcgaaga tgtagccttc acagttgaac gcgctgcagg tcaagtatgt gaccgttgcc
    34021 gtcgtatcga cccaacaaca gcagaacgca gctaccaggc agtcatctgt gaccactgtg
    34081 caagcatcgt agaagaaaac tttgcggaag cagtcgcaga aggatttgaa gagaaataag
    34141 gttgaaaagt ctaggcaaaa ttcaatttga gaagaaaaga caactaattt tatagtctat
    34201 taaacgcatt gtatcacgtt tttgaatacc tgatatgatg cgttttttat ttattttaaa
    34261 aatttgcgag gtatgacttt ttatactcaa caagaatcaa agagaaactt agcaagctaa
    34321 cagtagtaag ataaaatagg aatttgatat tagggataag attgctaaat a
//
LOCUS       DAYYQN010000017        34295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYYQN010000017 DAYYQN010000000
VERSION     DAYYQN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.017"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            75..1967
                     /gene="pepO"
                     /locus_tag="ACHBRQ_000747"
     CDS             75..1967
                     /gene="pepO"
                     /locus_tag="ACHBRQ_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HFM9592455.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2090..2719)
                     /locus_tag="ACHBRQ_000748"
     CDS             complement(2090..2719)
                     /locus_tag="ACHBRQ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9592456.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3715..5937
                     /locus_tag="ACHBRQ_000749"
     CDS             3715..5937
                     /locus_tag="ACHBRQ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HFM9592457.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5967..6410
                     /gene="dtd"
                     /locus_tag="ACHBRQ_000750"
     CDS             5967..6410
                     /gene="dtd"
                     /locus_tag="ACHBRQ_000750"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9592458.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6882..7296)
                     /locus_tag="ACHBRQ_000751"
                     /pseudo
     CDS             complement(6882..7296)
                     /locus_tag="ACHBRQ_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7427..8073)
                     /locus_tag="ACHBRQ_000752"
                     /pseudo
     CDS             complement(7427..8073)
                     /locus_tag="ACHBRQ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0034258 - nicotinamide riboside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            complement(8250..8900)
                     /locus_tag="ACHBRQ_000753"
     CDS             complement(8250..8900)
                     /locus_tag="ACHBRQ_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9592459.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            9012..9854
                     /locus_tag="ACHBRQ_000754"
     CDS             9012..9854
                     /locus_tag="ACHBRQ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HFM9592460.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9930..10367
                     /locus_tag="ACHBRQ_000755"
     CDS             9930..10367
                     /locus_tag="ACHBRQ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9592461.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10435..10737
                     /locus_tag="ACHBRQ_000756"
     CDS             10435..10737
                     /locus_tag="ACHBRQ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592462.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10828..11901)
                     /locus_tag="ACHBRQ_000757"
     CDS             complement(10828..11901)
                     /locus_tag="ACHBRQ_000757"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HFM9592463.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEVFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            12057..12734
                     /locus_tag="ACHBRQ_000758"
     CDS             12057..12734
                     /locus_tag="ACHBRQ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9592464.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12727..13701
                     /locus_tag="ACHBRQ_000759"
     CDS             12727..13701
                     /locus_tag="ACHBRQ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFM9592465.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13750..15693)
                     /gene="thrS"
                     /locus_tag="ACHBRQ_000760"
     CDS             complement(13750..15693)
                     /gene="thrS"
                     /locus_tag="ACHBRQ_000760"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9592466.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15751..16074)
                     /locus_tag="ACHBRQ_000761"
     CDS             complement(15751..16074)
                     /locus_tag="ACHBRQ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HFM9592467.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(16505..16705)
                     /locus_tag="ACHBRQ_000762"
     CDS             complement(16505..16705)
                     /locus_tag="ACHBRQ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592468.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            16942..17571
                     /locus_tag="ACHBRQ_000763"
     CDS             16942..17571
                     /locus_tag="ACHBRQ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9592469.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17712..17981
                     /gene="rpsO"
                     /locus_tag="ACHBRQ_000764"
     CDS             17712..17981
                     /gene="rpsO"
                     /locus_tag="ACHBRQ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9592470.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18796..19410
                     /locus_tag="ACHBRQ_000765"
     CDS             18796..19410
                     /locus_tag="ACHBRQ_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFM9592471.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19702..20451)
                     /locus_tag="ACHBRQ_000766"
     CDS             complement(19702..20451)
                     /locus_tag="ACHBRQ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9592472.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDCSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20584..22920
                     /locus_tag="ACHBRQ_000767"
     CDS             20584..22920
                     /locus_tag="ACHBRQ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9592473.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKLINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23022..23699
                     /locus_tag="ACHBRQ_000768"
     CDS             23022..23699
                     /locus_tag="ACHBRQ_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9592474.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            23686..24483
                     /locus_tag="ACHBRQ_000769"
     CDS             23686..24483
                     /locus_tag="ACHBRQ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9592475.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24500..25603
                     /locus_tag="ACHBRQ_000770"
     CDS             24500..25603
                     /locus_tag="ACHBRQ_000770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9592476.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            25610..25801
                     /locus_tag="ACHBRQ_000771"
     CDS             25610..25801
                     /locus_tag="ACHBRQ_000771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592477.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            25904..26923
                     /gene="galE"
                     /locus_tag="ACHBRQ_000772"
     CDS             25904..26923
                     /gene="galE"
                     /locus_tag="ACHBRQ_000772"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9592478.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26925..27890
                     /locus_tag="ACHBRQ_000773"
     CDS             26925..27890
                     /locus_tag="ACHBRQ_000773"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9592479.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(27941..28147)
                     /locus_tag="ACHBRQ_000774"
     CDS             complement(27941..28147)
                     /locus_tag="ACHBRQ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9592480.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28199..28675
                     /locus_tag="ACHBRQ_000775"
     CDS             28199..28675
                     /locus_tag="ACHBRQ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9592481.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28684..29355
                     /gene="cmk"
                     /locus_tag="ACHBRQ_000776"
     CDS             28684..29355
                     /gene="cmk"
                     /locus_tag="ACHBRQ_000776"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9592482.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29486..29824
                     /locus_tag="ACHBRQ_000777"
     CDS             29486..29824
                     /locus_tag="ACHBRQ_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9592483.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29827..30456
                     /locus_tag="ACHBRQ_000778"
     CDS             29827..30456
                     /locus_tag="ACHBRQ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HFM9592484.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30696..31847
                     /locus_tag="ACHBRQ_000779"
     CDS             30696..31847
                     /locus_tag="ACHBRQ_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9592485.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32160..32909
                     /gene="truA"
                     /locus_tag="ACHBRQ_000780"
     CDS             32160..32909
                     /gene="truA"
                     /locus_tag="ACHBRQ_000780"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9592486.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            32899..33672
                     /locus_tag="ACHBRQ_000781"
     CDS             32899..33672
                     /locus_tag="ACHBRQ_000781"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9592487.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33650..34111
                     /locus_tag="ACHBRQ_000782"
     CDS             33650..34111
                     /locus_tag="ACHBRQ_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9592488.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         9863 a         6476 c         7881 g        10075 t
ORIGIN      
        1 taaatggtta ttaattgcat tccctagtga tttttgttaa gataaatgca aatacaaatg
       61 aaagcgagaa caagatgaca cgttatcaag atgattttta tgatgctatc aatggagaat
      121 ggcaacagac agctgaaatc ccagcagata agtctcaaac aggaggtttt gttgatttag
      181 accaggaaat tgaagacctg atgctggcga caacagacaa gtggttagca ggtgaagaag
      241 tgcctgagga tgctatcttg gaaaactttg tcaaatacca ccgcctagtt cgtgattttg
      301 acaagagaga agctgacggt atcacacctg tcttaccact ccttaaagaa ttccaagaat
      361 tggaaacttt tgcggatttt acagctaaac tagcagagtt tgagcttgca ggaaaaccaa
      421 acttccttcc ttttggtgta tcgccagact ttatggatgc tagaatcaat gttctatggg
      481 ctagcgctcc aagcacaatc ttgccagata cgacctacta tgcagaagaa catcctcagc
      541 gcgaagagct cttgactctt tggaaagaaa gcagcgcaaa tctcctcaag gcttatgatt
      601 tctctgatga agaaattgaa gacttgctag aaaaaagact tgaattggac cgccgagttg
      661 cggcagtggt gctctctaat gaagaaagtt cagaatatgc taaactctat catccatatt
      721 cttacgaaga tttcaagaaa ttcgcgcctg ccctaccttt ggatgacttc ttcaaagcag
      781 ttattgggca attaccagac aaggttattg tagacgagga acgtttctgg caagcagcag
      841 agcaatttta cagtgaggaa gcctggtctc tccttaaagc aaccttgatt ttgagtgttg
      901 tcaatctttc aaccagctat ttaacagagg atatccgtgt tttgtctggt gcctacagcc
      961 gtgccctttc tggagttcca gaggcaaaag ataaggtcaa agcagcttat catctagcac
     1021 aggaaccttt caagcaagcc ctggggcttt ggtacgcccg tgagaagttc tctccagaag
     1081 ccaaggcgga tgtggagaaa aaagtggcaa ccatgattga tgtctataag gagcgtctgc
     1141 ttaagaatga ctggctcact ccagaaacct gtaaacaggc tatcgtgaag ctcaatgtga
     1201 tcaaacctta tattggctat ccagaagaat tgcctgcacg ttacaaggat aaggtagtga
     1261 atgaaactgc cagtcttttt gagaatgctc tagcctttgc gcgtgtggaa atcaagcaca
     1321 gttggagtaa gtggaaccag cctgtagact ataaggaatg gggcatgcct gctcatatgg
     1381 tcaatgccta ctacaatcct cagaagaacc tgattgtctt tccagcggcc attttacagg
     1441 cgcctttcta tgacttgcat cagtcatctt ctgctaacta cggtggtatt ggggcagtga
     1501 ttgcccatga aatttcccac gcctttgata ctaacggggc ttcctttgac gaaaatggta
     1561 gcctcaagga ttggtggaca gagagcgact atgctgcctt caaggagaaa acacaaaaag
     1621 tcattgacca atttgatgga caggattctt atggagcaac cattaacggt aaattgactg
     1681 tatcagaaaa cgtggctgac ttgggaggaa tcgcagcagc gcttgaagca gctaagagag
     1741 aagcagactt ctcagcagaa gagttcttct acaacttcgg tcgcatctgg cgcatgaaag
     1801 gtcgaccaga atttatgaaa cttttggcta gcgtcgatgt gcacgcacca gccaaactcc
     1861 gtgtcaatgt gcaagtacca aacttcgacg atttctttac aacctatgat gtcaaagaag
     1921 gagacggaat gtggcgttca ccagaggagc gcgtgattat ttggtaatac aaaaaatcta
     1981 gtcatcagaa aaaaggcatc caatcacgtg tgaaaacctt gataggatgc cttttgtaat
     2041 agaaagattt gctggatagt ttacttaaat ttcgattgtc atcatcttgc tagaaaaagg
     2101 gattgaaggc cttttcgtga gcgatagtag tagctggacc atgtcctgga tagacatcgt
     2161 agtttggtag ggtgaagagt tgggtctgga tactatgaag gagttgctcc atgctaccag
     2221 tcggaaggtc ggtccgtccg atagtttcgc ggaatagagc atctcccgtc aagactaggt
     2281 gagcatcagg aaagactagg gaaacaccac cgatagagtg ccctggggtc ggtagaacct
     2341 taaaacgaaa ttcctctagt tggtattctt cgtgaaagac aaaggtgtgt tctgcaggtt
     2401 ttgtgaccac atctgccata tcatcgtggc gagggagacc ggagagatta tcgacaggag
     2461 tgtagagcca gctggcttcg ctctctgcga tatagacagg aggattgcca aacgtctcgc
     2521 gaaccaagtc cagactcatg atatggtcat aatgggcgtg ggtcaagaga atagcacaga
     2581 tcggtttgtt gatcttctcg attgtctgac gaatggcttc ccaatgacta ccaggatcga
     2641 cgacgatgag gtgcttttcg ccttctagat aataggtatt ttcataggca acaggattca
     2701 cggttttatg gattttcata ctagctccaa tctcaaagaa tgtactaata ctcttcgaaa
     2761 atctcttcaa actgcgtcaa ctttatctgc aacctcaaag cagtgctttg aacagcctgc
     2821 ggctagcttt ctagtttgct ctttgatttt cattgagtat aaattaagta tagcacagtt
     2881 agggagaata ggtaaggatt taaaatgaac ttaaaaacca gcatgactag atgaagacag
     2941 gctggttatc aatttatcaa tattggaaag gtgtcaattg cccttcatag gttgcataaa
     3001 ctccgtcatt agcttgtttg atagacgtga tgtttagagt gctaccgtag ttggcttctc
     3061 gtttgtcgct atattcacta taagttatcc tactgggcag gttctggtcg ttagcatagt
     3121 attgaacgac tgttttttat agctttgctg gctgaatagg aacaggcagc aaactcctaa
     3181 cattaggaga gcaaagatag aaatagcagt ttttttcata ggtattctcc ttataaacta
     3241 aaggatagaa gaaagtatct tgaggatttt tatactcttc gaaaatctct tcaaaccgcg
     3301 tcaacgtcgc cttgccgtat atatgtgact gactttgtca gtcttatcta caacctcaaa
     3361 gcagtgcttt gagcaacctg cggctagttt cctagtttgc tctttgattt tcattgagta
     3421 ttaattccaa aatccagtca aggtaccagt gtatgtgata cgagttccac cgtttatctt
     3481 tcgtatcttc aatggaattg gggaaatcag aaagtctttg ggttacaata tggtttccgt
     3541 tcttctatct ctccttctat cacctaagtt taaaatctaa cttcgtagta tattatgggc
     3601 cgataattca gaattatcaa gcaattttga attatttttt tcttattctg ttccaatcta
     3661 gttttcatat tcttaaaaaa gtgataaaat ggtaggaaga attggagagt agagatgccg
     3721 aaagaagtga atttaacagg cgaagaagtt gtcgctttaa ccaaagaata tttaacggaa
     3781 gaggatgttc attttgtcca taaggccttg gtctatgctg ttgaatgcca cagtggtcaa
     3841 tatcgcaaat caggcgagcc ttatatcatt caccctatcc aagtggcagg tattttagct
     3901 aagctaaagc tggatgctgt aacagtagct tgtggattct tgcatgatgt ggtggaagat
     3961 acagatgcga ccttggacga tttggaaaga gagtttggtc ctgatgtgcg ggtgattgtt
     4021 gacggagtta ccaagcttgg caaggtcgag tacaaatcaa tcgaggagca attagcggaa
     4081 aatcatcgca agatgctcat ggccatgtct gaggacatcc gcgttatttt ggtcaaactg
     4141 tctgaccgct tgcacaatat gcggaccctg aaacatcttc gaaaagacaa gcaggagcgt
     4201 atttccaaag aaaccatgga aatctatgcc ccacttgccc atcgtttggg gatttccagt
     4261 gtcaaatggg aattagaaga cttgtctttc cgttatctca atccaacgga gttttacaag
     4321 attacccata tgatgaagga aaagcgcagg gagcgtgagg ccttggtgga tgaggtagtc
     4381 acaaaattag aggagtatac gacagaacgt cacttgaaag ggaagattta tggtcgtccc
     4441 aagcatattt actcaatttt ccgcaaaatg caggacaaga gaaaacggtt tgaggaaatc
     4501 tatgatctga ttgctattcg ttgtatttta gatacccaaa gtgatgttta tgccatgctt
     4561 ggttacgtgc atgaattttg gaaaccgatg ccaggtcgct tcaaagacta tattgccaac
     4621 cgcaaggcca atggttatca gtctatccat acgactgttt atgggccaaa agggccgatt
     4681 gaattccaga ttcgaaccaa ggaaatgcac gaggtggctg agtacggggt tgcggctcac
     4741 tgggcttata agaaaggtat aaaggggcaa gttaacagca aggaatcagc tattggaatg
     4801 aactggatca aggagatgat ggagctccaa gaccaggctg atgatgctaa ggaatttgtg
     4861 gactctgtta aggaaaacta tctggctgag gagatttacg tttttacccc agatggagct
     4921 gtccgttccc ttcccaaaga ttcaggaccg attgattttg cctacgaaat ccataccaag
     4981 gtcggtgaaa aagcaactgg tgccaaggtc aatggccgca tggttccact gacaaccaag
     5041 ttaaagacag gggatcaggt tgaaattatc gccaacccga actcctttgg acctagccgt
     5101 gactggctca atatggtcaa gactagcaag gcgcgcaata agattcgcca gttctttaaa
     5161 aaccaagata aggaattgtc tgtcaacaag ggtcgtgaga tgctgatggc tcagttccaa
     5221 gaaaatggct atgtggcaaa taaatttatg gacaagcgcc acatggatca agttctgcaa
     5281 aagaccagtt acaagacaga agactccctc tttgcggcca ttggttttgg ggaaatcggt
     5341 gcgattaccg tctttaaccg tctgactgaa aaggaacgcc gtgaggaaga gcgtgccaag
     5401 gccaaggctg aggcagagga gcttgtcaaa ggtggcgagg tcaaggttga aaataaagaa
     5461 actctcaagg tcaagcatga ggggggagtg gttattgaag gtgcttctgg tctcctagtg
     5521 cggattgcta agtgttgtaa ccccgtgcct ggtgacgata ttgttggcta cattaccaag
     5581 ggtcgtggtg tggctattca ccgtgtggac tgtatgaacc tgcgtgccca agaaaactac
     5641 gagcaacgtc tccttgatgt ggaatgggaa gaccagtact ctagctcaaa taaggagtat
     5701 atggcccata tcgatatcta cggtctcaac cgtacaggac tgttgaacga tgtactgcaa
     5761 gttctttcaa atacaaccaa gaatatttca acggtcaatg cccaaccaac caaggatatg
     5821 aagtttgcta atatccatgt gtccttcggt attgccaacc tctctacact gaccacggtt
     5881 gtcgataaaa ttaagagtgt gccagaagtt tactctgtca aacggaccaa cggctagttg
     5941 tcctagctct tactagaaag gctattatga aaatcattat ccaacgggtt aaaaaagccc
     6001 aagtgagtat agaaggccag attcagggaa aaatcaatca gggactttta ttgctagttg
     6061 gtgttggacc agaggaccaa gaggaagatt tggactatgc tgtgagaaaa ctggtcaata
     6121 tgcggatttt ttcagacgca gaaggcaaga tgaacctgtc tgtcaaagat attgaaggag
     6181 aaatcctctc tatttctcag tttaccctct ttgcggatac taagagaggc aatcgtccag
     6241 cctttacagg ggcagctaaa cctgatatgg catcagactt ctatgatgct ttcaatcaaa
     6301 aattagcgca agaagtgccc gttcagacag gtatctttgg agcagatatg caggttgagc
     6361 tggttaataa cggacctgtt accattatcc tagatactaa aaagagataa gaaagaccaa
     6421 gcccagtcgg cttggtcttt ctcatctatc ataaaatact ccaaaaagaa atcggttctt
     6481 gatatgcttg ggggaagtct cttttcaggc tttggcagat gcgataggaa gggatgagat
     6541 gtcctaggat gaggagagtt ccctgaagga aaagtggaat atcacttaaa gacaccaagg
     6601 agagaatcat caggctatcc cataggagaa tttgtaaagc aaaacgccag aatctcggtc
     6661 taatctggga atagagttga ctaaagtggt cacaaagttg gtttgaaaga taggtcaata
     6721 gcacaggatg aaagaaaatg agccaaccgc tataaatcaa aatccagtcc caagtaagcc
     6781 ccccttttaa atgtcaaaaa tgccagcatg attccatcaa tgacaaggag ttgaatggtt
     6841 ttgatgaaga tgattttttt catgataaaa cctccttttc cttagagaca ctcccataaa
     6901 gagatgtgtt tgtcgtaaaa gtctggatag ttttctctta ggtagtcagc tgtcatataa
     6961 taaaaagtag aatggcaagc agtgataatt gacataagag atgaaaaacg ctgagaacta
     7021 atgaaggcta agagaacaaa aaaatagaaa atcaatggtg agaaccccta aatagtagaa
     7081 gcgaatcttt ttattgattt gaggataaat ctcagcgaat tgatgtttga gtcggacgat
     7141 caagagaaat agcaggagtc cttgagcaag gaagaaaatt aaaccagaga gcaagagcca
     7201 ctcaaaagat gtctcgagtc taatgataaa aaatgcaaat agtagcaaat caataactgc
     7261 aattgctata aaatggattc tatagagttt tttcatgaca agacctccct cttttatcta
     7321 acttcattct actctaaaag aatgggagtt acaactaaaa tgataaaaat agcagagggg
     7381 agattctctt aagttgacta gtattcttta tttgagtttc cttctattat ctaacttctt
     7441 catcattcca gacaaataaa gctccgattg cattgaggat ataaaagatg tatttaccga
     7501 tattggcgaa gtttccttga ataccagctt ttgtcagctg aacgaaattg taaatcaacc
     7561 aaaagcccca ctgtccctca ttgccaatga attcaaagca ttggcaatga gggacagtgc
     7621 aaaggcaata gttgttacgt aggcaaggag attcatcttg cccccatatc cgatatagtt
     7681 ggtcacaaag gcaaagagga aggcgatgat ggaaatgatg atggccgcca attttacctg
     7741 tttttggctc atttggttgg gtctgccttc ttgcgaagct ttccatttct ttatagcaaa
     7801 ggtataaatg aggaaggtga cgggataggt aatgatggcc gccttatttc caaggatata
     7861 atcaatagca ccggacaaaa tggtattaac aataccaaag taatttcccc atttgcttaa
     7921 tttccccgtg aaacgagtgg acaacatgga aatcccaacg ttggttacgg aaatcaatcc
     7981 aaagggtaca agagctgtcc atagtcccca gtctacaaat ttatcgaggc gtgagttgag
     8041 ataaccagat gcaatcgcaa tcccaacgac caaagcaacc ccgaagaggt caaatcattt
     8101 agatgtagca aaaattttta gtgatttttt cataggttaa actacctttc tttttttcaa
     8161 atactctacc accaaatgaa agtaaaatca aatgatagaa ataaaaccct gaaaataaaa
     8221 ctttcagggt tggtagggga cttgagaaat tagttgattt tctcgacata gagttgtttt
     8281 gcaatgtcca tattcacttg taaatcctcg tcgttactga ggatagtgaa ggtattgctg
     8341 aagccatcaa actgcttgac ttggagctgg tcaccgatgt gaagtgagtg cttgtccaga
     8401 taatggagaa tgtcaaaact atcgtgcacc cgagtcaggc ggtaggcgcc agcttccttg
     8461 atatcagcta gtgggaggtt attgatttca acgaggagtt ctcccttggc aggaatagtt
     8521 cctccgtggg ggcaggtttt agggaaacct agcagtttat ctagtctttc cacgaacagg
     8581 tcagagacag tgtgttccaa gacctcagct tcctcgtgaa tctggtcact tgtatagtct
     8641 aaatgatgaa ctagaaaaac ttcaatcaag cggtgcttac gatagagctc agagaccagt
     8701 ttgagaccga ggtcagtcag tagatagcca cattccttgt cctttaggat gagattttca
     8761 cttttcattc gtttgatcat ttcagttacg gcagggggag agacttgcat gcgagccgca
     8821 atttccttgt tggtaatctt atgcaggtct atgccaattt cataaataca ttttagatag
     8881 tcttctttgt ttggggtcat tcgtttcctc ttgtctaata tctttatcta gtatatcaaa
     8941 aaaagctaga tttctctagc tgtgactttt tatgttatac ttattgcaag agaaaaaacg
     9001 aggagatgga tatgacgaaa atagctcttc tttcagatat tcatggaaat accaccgcct
     9061 tggaggctgt tttggcagat gctcagcaga taggagtgga tgaatactgg cttttgggag
     9121 atattctcat gccagggaca ggacgtagaa ggattttgga cttgttggat caactaccga
     9181 ttacggctag agttttggga aactgggaag acagtctttg gcatggtgtc cgtaaggaat
     9241 tggacagtac tcgccccagt caacgctatc tcttgcgcca gtgccagtat gttttagagg
     9301 aaatttccct agaagaaatt gaagtgctcc acaatcaacc tcttcaaatt catcgtcagt
     9361 ttggggattt gacggtggga attagccatc atctgcctga taagaactgg gggcgagagt
     9421 tgattcatac tggcaaacaa gaggagtttg accgcttggt gactcatcct ccctgtgata
     9481 ttgctgttta tggacatatt caccagcagt tgcttcgtta cgggactggt gggcaattga
     9541 ttgtcaatcc gggttcgatt ggccaacctt tctttttgga cgctcagcta cggaaggact
     9601 tgcgggccca gtatatgatt ttggagtttg atgacaaagg cctggtagat atggacttcc
     9661 gacgggtaga ctacgatgtg gcagcggaat tgcagctggc taaagacctg agacttccct
     9721 attttgaggt ttactatgaa agtctggtca atgggatcca ccatactcat catcaggaat
     9781 ttttgagaga attggctcag aaagagggct gcgatcggga gttagacgac tggttgaaaa
     9841 gtggtaacga ttgacattac aactaaaaag ggtataataa tagaaaaaga agagtagagg
     9901 cagggtagcc tcgtaaaaag gagaagagga tgcaaattcc aagtagattt accattgcga
     9961 ctcatatgct gataatcatt gccctcgagg ggaaggaaag caaggtgacc agtgattttc
    10021 tggctgctag tgtcggggtc aatcctgtca ttatcagaaa gatcttgtcc cagttgaaga
    10081 aggcagagct gatttcagta gcgcgtggaa cgggcggaac agagattgtc aaggacctta
    10141 aggatattag tcttttagat gtttatcagg cggtcgaatg tcttggtaag acaggtcaac
    10201 tcttcagttt ccatgacaat ccgaatccaa attgccctgt aggagctcat attcatgatg
    10261 ttttggatca aaaattggag agaattcagt tgactatgga ggcagaactt ggtcaaacca
    10321 gtctagaaaa agtcgtggcc gatgcagaga gtcagatgaa ggattaagag ggataaatgc
    10381 cctctttttt ctttggataa ttatgataaa atgtagctat taaaggagta agaaatgtat
    10441 ctcgttatag gaattgtatt agcctttata gtgtcatttt ggaaggataa tagaagtttg
    10501 tggaatccag tattattttt attatccctc atttcaagtt atttttatct atcctacctt
    10561 ttttataaaa atggatacga gaatgttcag ttagcttttt atgtatttgc ctttgtctta
    10621 cttccttttt tgctttttct aagtggcatc tttttaatct ataatggggt cattttgttg
    10681 aagcgagagg gacgttccaa gccccattat ctctcaatgc tctttgattt ttattgacta
    10741 ttagattcta tttaaaaaga cttagaatgc acatgaaagt atgtattcta ggtcttttgt
    10801 tttgtataca tttaattttc aagagtatta tctatttttc catgcctggt aacgagtatc
    10861 aatcaataac tgggtaaggc gacccaaggt ttctctttct tctggagtga gggattgagc
    10921 ttggacaaag agatgatgaa tgtattcaag cgcctttaag gaagccaagt cattgataga
    10981 gctaatacca gcctcaagaa tagtgccaaa gaagaggtcg aagtagagct gaagttcttc
    11041 gtcagggact gtggccaccc attctttaaa ggttgtgtct acttgctggc tatcactgtt
    11101 ggtcttatcc agttggacga agtgcttgtc ctcaatctgc caactaaagg tatcgtgctg
    11161 ggcgatgcca cccagggcag tactctgaac gatgatttgg tgagcaggaa tttccagcat
    11221 cataccgata atggaacctt gtggaatgaa tatcttgctt ctatccatta tcctttgata
    11281 acccgcagtc tgtgtcaatt cttgatggag accaggtgca tcaaatgtat aaactgctgt
    11341 gatctgattt tgcaaacttt gctcaatttg gctagcagca tagatagcga gatttcctcc
    11401 cttggaatgc ccagctagaa taaccttttg cttaggatga tgggcaaaaa agttctttaa
    11461 atagcgaagg gcgtgctttt gagcaggaat ttccttcata taggtcaggt ggaaaacttc
    11521 cttccagcca atgatactgt catctgtccc acgaaagaca atcagatagg tatcgaggct
    11581 gacacgataa gtcatagccg caaattgctt ttgcagttca gggtcgatgt cgttgataaa
    11641 atgggagagt ttgcaatttt tgaagcgctt gtgttgagcc aattcatcta ataattgaag
    11701 gcgatttttg ctagtaagca tggtgggatc tcttggaacc tgaggtgcta gatctaaaag
    11761 acgttgagga agtgtggaga ccagattatc aaaggagagg taggtgattt ctattaaggt
    11821 tagaatgtct aactcattca agggaaggtc gtaataagaa tcatatgcga catctttcag
    11881 atagtcaaaa atattggcca taagagctcc tttcttttta tttatcttag caaatttccc
    11941 tcaaattagc tagtagcatt gcctgtttgt actggctaaa aacaggctat ttcaaattca
    12001 gtttcagacc atctggcatg gaaaaatctg ttataataat agaaaaggag aagcgcatgc
    12061 acaagatttt attaatagaa gatgatcagg tcattcgtca acaggtcggg aaaatgctct
    12121 ctgaatgggg atttgaagtg gtcctggtag aagactttat ggaagttttg agtctatttg
    12181 ttcagtcgga acctcatctg gtcctcatgg atattggttt gcccttgttt aatggttatc
    12241 actggtgtca ggaaatccgc aagatttcca aggtacctat catgtttctt tcttcgagag
    12301 accaggctat ggatattgtc atggcaatca atatgggggc ggatgacttt gtgaccaagc
    12361 cttttgacca gcaggttctt ttagctaagg ttcagggctt gttgcgtcgt tcctatgagt
    12421 ttgggcgtga tgagagtttg ctggaatatg ctggtgttat cctcaatacc aaatccatgg
    12481 atttacatta tcaagggcaa gtcttgaatt tgaccaagaa tgaattccag attttacgcg
    12541 tgttatttga gcatgcaggc aacatcgtag cacgtgacga cctgatgcgg gaactttgga
    12601 acagtgactt tttcattgat gataataccc tctctgtcaa tgtggctcgt ttgcgtaaaa
    12661 agttggagga gcagggattg gtaggattta tcgagaccaa gaaaggaata gggtacggat
    12721 tgaagcatgc ttgattggaa acaatttttt ctagcctatc tgcgctcccg tagtcgtctt
    12781 tttatctatc tgctttcttt ggcatttctt gtcttactct ttcagttttt atttgccagt
    12841 ctaggaattt acttcctcta ctttttcttc ttgtgttgct ttgtaaccat cttatttttc
    12901 acttgggaca tattggtgga aacgcaggtc tatcgccagg aacttctcta tggagagagg
    12961 gaagccaagt ctcctttgga aatagcttta gcagaaaaat tagaagcgcg tgagatggaa
    13021 ctctatcagc agaggtcaaa agcagaaaga aaactgacgg atttgctgga ttactatacc
    13081 ttgtgggtcc atcagataaa gacccccatt gcagccagtc aactcttagt tgcagaagtg
    13141 gtcgaccgcc aactgaagca gcagctagaa caggaaattt tcaaaatcga ctcctatacc
    13201 aacctagttt tacagtacct gcgtttagaa agtttccatg atgatttggt cttaaagcag
    13261 gttcaaattg aggacttggt caaggaaata attcgtaaat atgctctttt ctttattcaa
    13321 aaaggcttaa atgtcaatct acatgacctt gataaagaaa tcgtgacgga taaaaagtgg
    13381 ctgctagtgg ttattgagca aatcatctca aacagtctca agtacaccaa ggaaggtggt
    13441 ctggagattt atatggatga ccaagagctt tgtatcaaag atacgggaat cgggataaaa
    13501 aacagtgatg tcctccgagt atttgaacgt ggcttttcag gatacaatgg ccgtttgacc
    13561 cagcagtcct ctggacttgg cctttatcta tctaagaaaa tttctgaaga actggggcac
    13621 cagattcgta tcgagtctga ggtcggaaaa ggaacgacag tgcggattca gtttgctcaa
    13681 gtgaacttag tccttgagta aaaatagatg agaaaaagcc tccagattgg aggcttttat
    13741 gctagactct tatttctcaa cgcgtgattt gttggcgata tcagctagga tagcttgaac
    13801 aaaattatca actgagacag tttgtgtttc tttttggccg tagcgacgaa cgttgactgt
    13861 ttcgtcttcc atttctttgt ctccaacaat taattggtaa ggaatcttgc tggtttgtga
    13921 agcacggatc ttgaactgca ttttttcatt gcgctcatct acgtctgcac ggacaccgcg
    13981 gtcacggagt ttcttggcta cttcccaagc gtagtccacg tgtttttcgt tagatactgg
    14041 gataagggtt acttggtgtg gtgccagcca tgttgggaag gcacccttgt agttctcaat
    14101 caagatagct gtgaagcgtt ccatagttga gataacccca cggtggatca tgactggacg
    14161 gtgatcttcg ccatcagctc cgatgtattt gaggtcgaag cgttctggca acaagaaatc
    14221 aagttggata gtagaaaggg tttcttcttt tccaagggca gttttaatct ggatatccaa
    14281 ttttggtccg tagaaggccg cttcaccttc ggcttcaaag tagtccacgc ccatttcatc
    14341 aagagctgca cgaagcatgg tttgggcatt ttcccacatc tcatcgttat caaagtactt
    14401 atgagtatct tgagggtcac gaagagagag gcggaagcgg tagtcagtca agttgaagtc
    14461 ttcataaaca tcgataatca actgaagggc acgttggaat tcttcttgga tttgttctgg
    14521 agtaacgaat aggtgaccgt cgttgagtga catttcacgt acacgttgaa ggccagtgag
    14581 ggcaccagat ttttcgtaac ggtgcatcat accgatttca gcgatacgga ttggcaattc
    14641 acggtaagag tgaacatggt gtttgaaaac ttggatgtgg tgcggacagt tcattggacg
    14701 aaggacaaat tcttccccgt cacccatgtc catggttggg aacatgtctt cttggtaatg
    14761 atcccagtgg ccagaagtct tgtaaagctc aacagaagca agtggtggag tgtagacgtg
    14821 ttggtagcca gaagccaact ctttgtttac gatgtagcgt tccaattcac gacggatagt
    14881 cgcaccattt ggcaaccaga atggcaaacc ttgtcccact tcttgtgaaa tcataaagag
    14941 gtcaagctct ttaccaagtt tacggtggtc acgttcctta gcttcttcac gcatttgaag
    15001 gtagtttttc aagtctttct tttcaaacca agctgtaccg tagatacgtt gcatcatagc
    15061 gttgtcgctg tttccacgcc agtacgcacc agctacatgg agaaggtgga agatttggat
    15121 acgacctgtt gatggaacgt gaggtccacg gcagaggtct acatattcac cctgacgata
    15181 gatagtcaaa ccgccttcgt cttctgagtg ttcttcaatc aattccaact tgtaagggtc
    15241 atttttgaag atttcacgtg cctcgtcttt agtcacttct tcacgaatag atgggaagtt
    15301 ttctttgacg attttttgca tttcttcttc gatacgagga aggtcttcgt tagagatttg
    15361 accagctgtg ttgtcagtat cgtagtagaa accatcttcg atggctggac caactcccaa
    15421 gtgaatgtct gggaaaagac gacgagctgc ttgggcgaac aagtgagctg ctgagtggcg
    15481 caagattgga agggcatctt cgtgatcagg tgtcacaatt tcgatgcttc catcttcagt
    15541 gatagcgcga gtagtgtcga tgagtttgcc gttgaattta ccagccaagg cttttttagc
    15601 tagggaattg ctgatagatt gggcaatttc aaaagttgtt acgccagatt cgaattcacg
    15661 aacagcgcca tctgggaaag taatgttaat catgatgttc tcctttttta ttatttcata
    15721 gtttctaagc attgattttt tatcatcaga ctacttccaa gtcctaacgt tcaattgctt
    15781 ttctttatca gagaggaagt tttccatttc cgtcagaatg tctatatcca aacgttgaga
    15841 aagttctaat agccaccaga tattttctga aagtttttgt tccagtgtgt agggtgtttc
    15901 atcatagtag cgtccttgct ttgtcatcac cagtcgttgg aaatttccaa tatcattaga
    15961 taaagccaag gggtcttctt ctaccgtcca cttggaatca tgatgcttaa cttccagttc
    16021 gtgataagct tgtcggattg cccaactgcg ctccactaat tcttgcaact ccataattag
    16081 tatcctaata aaaaaatgcg acatcctcga aaggacgtcg caacgtggtt ccaccttcat
    16141 ttatgtacct caaaagagag ggacacctct gattggctct aacgtggcca ccgttttatg
    16201 ttttcataaa aaactcaaga gtagtatcag tttagacttc ctatgcgttt ccagcaacca
    16261 cgcactctct agtagaaagg gactaagtga cttgtctcta tggattatta tagcatgatt
    16321 gtgagatttt tcaaggattt tgtgaaagtt ttttgttttt cattttttgt agaatatgct
    16381 tgatgctaat accagaatcc atgcccacca gagcaactgt ttcatagatt agcaagtaaa
    16441 ttagtataaa ctcaccaaag gttatgatag taaagtaaaa gagcagtccg ttaccaacta
    16501 accatcaaag tgagaaacgg aaacggtagc tatagagtaa cgatacaatc aagattacca
    16561 gaggtgtaaa gaagagaaga ttgtttatat agagttcctt gagtgtaagg gggtcagaaa
    16621 caactagtaa gaacaactca taaagtggcc aataaaagaa taatatgacc agatagtagg
    16681 ggagatggga atagaatcta cgcatgataa tcacctcgtt ttaaaaaaac agacaactta
    16741 gatctcttgc tatgattatt atagcatgtt taaaacaaga aagtaaattt gttggcaaag
    16801 aagatttttt ttcactagaa tcatggttag ttttcttggt agaaatattt tgataataat
    16861 taagcaaaac tattgacatt gattattaag agtgtcacaa ttgttgtaac taaaataatt
    16921 acaataagag aggtataaaa aatgaaacta gcagttattg cagcaaatgg acaagcgggg
    16981 aaagcaattg ttgaagaagt agtcaaacga ggacatgaag taacggctat tgttcgttct
    17041 gaaaacaaaa gtcaatctga gtctattatt aaaaaagatt tatttgagtt gacaaaagat
    17101 gatcttactg gatttgatgc tgttatttca gcgtttggtg cttatacacc agatacgcta
    17161 ccgctccaca gcaaatcaat cgaactattt aatcagcttc ttgctggaac acagacacgt
    17221 tttctagttg tagggggagc aggaagtctt tatatagatg aaacaaaaac gacacgactt
    17281 ttagatacgt ctgattttcc tgaagaattt aagtcactag caaaagctca agcggatgaa
    17341 ttggatcttc ttcgtacaaa aaataatctc aactggactt ttgtcagtcc tgctgtagat
    17401 tttattcccg atggtgaaaa aacaggaaac tacattctag caggagaaat ttttactact
    17461 aatgaaaaag gaataagcca gattagttat gcagattatg ctattggatt ggtagatgaa
    17521 cttgaaaaag gtcatcatat aaaagaacgt atctctttac ttgaaaaata ataactatga
    17581 gagtattaat gtttgacaga aaatttaaat cttggtaaaa tagggattgt cgtaaagaca
    17641 aataacttct tcttggttac aggcatgcca acctgtcact cggatgaagc caaataaaaa
    17701 ggagaaacat catggcaatc tcaaaagaga aaaaaaatga aatcattgca caatatgcac
    17761 gtcacgaagg tgatacaggt tcagtagagg ttcaagttgc tgtccttact tgggaaatca
    17821 accaccttaa cgaacacatc aaacaacaca aaaaagacca cgctacttac cgtggattga
    17881 tgaaaaaaat cggtcgccgt cgtaacttgc ttgcatactt gcgtaaaaac gacgttaacc
    17941 gttaccgtga gttgatcaac tctctaggac ttcgtcgcta attcaagata caaaggccgt
    18001 caaaaacaca aagcaaaaat aggaaaattg acgaagaaac ttcagtttct aggagatttt
    18061 atcttttttg ccaagtgctt aggccgtgtt caattgagca tatcttgata ataaagctac
    18121 tctaaattgg gtagctttgt tgcgttgtca ccttacctcg ctatactcaa gtataatctt
    18181 cggttacggt tcctagcact gtaaggtaaa ataaactagc ttttctccct tcggggagat
    18241 ttttttgttt cactgaaagt ttcgtacgat cttcacctcg tcccctagtc tataaacgat
    18301 ttatctagca agaatcatga tgctaagggc gtcaaaaatc cgtatgaaaa tagggaaatg
    18361 acacagtgtt cgatgagcac aaggagtttc atctttttca ctaggatttt agcccgggct
    18421 aaaatcagct ctctgacttc agagggcttt tttattgagg ttttatcggt aacaattttg
    18481 gagactacaa aaacctcaaa tggtatcagc taattacacc ataaaggtgc gcagttactc
    18541 ggcttttcaa gccgagtagc tgtatgcaag ccccctagaa gagcccactc tttacaaagt
    18601 aaagtatagt atgttatact ttacatggaa gtagtcactg aattccagtt agaaattact
    18661 ttgtaactac gttttgagga ggagtaaaat gctttcctac gttcgacatt acccactagc
    18721 gatagctaaa ttaatgtgtc tgtgctctcc taaaatctgc tgatttatta ctgactaata
    18781 caggaggttt tttttatggg acagacaatc atatctgcta ttggtgttta tatttccacc
    18841 agtatcgatt atttaattat tttaattatt ttatttgcac agctatcaca gaataaacag
    18901 aaatggcata tttatgcggg gcaatatcta ggcacaggct tacttgtagg ggcgagttta
    18961 gttgctgctt atgtcgttaa tttcgtgcct gaagaatgga tggttggatt gcttggttta
    19021 atccctatct atttagggat tcgctttgca attgttggag aagatgcgga agaagaagag
    19081 gaagaaatta ttgaaagatt agaacaaagc aaggcaaatc aactgttttg gacagttaca
    19141 ttgctgacaa ttgcgtctgg cggagataat ttaggtatct atatacctta ttttgcttcg
    19201 ttagattggt cacagaccct cgtggccttg cttgtgtttg taatcggcat aattatcttt
    19261 tgcgagatta gtcgggtgtt atcctctatt ccgttaatat tcgagacaat tgaaaaatac
    19321 gagcgaatca ttgtgccctt agtattcatt ctacttggac tatacatcat gtatgaaaat
    19381 ggcacgatag agacttttct gatcgtgtag atttttttgt ttcactagga ttttagcccg
    19441 agctcaaatc agctctctga ttttcagggg gctttttcat tgagtataaa tggacaatct
    19501 taaagttact ctattgtaga gtggcttttg atttgattag gagattctta taaatatagt
    19561 aaaatgaaat aagaatagga caaatcgatc aggacagtca aatcgatttc taaaaatgtt
    19621 ttagaagtag aggtgtacta ttctagttcc aatctactat ataaatgaaa accttagctg
    19681 tttggccaag gttcatttct tctatgcaag ttcttctctt ttcttatctg ggttccagat
    19741 gaagcttgcg ataaagctaa agatgatggt taggatagcg aggataatag caaggatgag
    19801 ggcagggatg cggataaacc accagagtgg atttggtttt ttatcattgt gccattgttt
    19861 cgtttcttcg tccagacgtt ggaattctgt ttgaatacga ttggcgactg tttcaatgcc
    19921 ttcatcattc attttcttga tatctgagat atcgattgga tttccaaagt tcatatcgac
    19981 acgttcacgg ctaatcaagc ccttcaaagt catgggaccg gtgtaggtaa ccggcatgat
    20041 acggaccttg gccattttgg caatcagtgc tgcgcccccc ttgacatcgt ttgagtggcg
    20101 gctaccactt ggaaacatga tgagagagca gtcacttttt ttgagaacgt tgataggata
    20161 tttgatggct gaggcgctgg gattttcacg gtcgatggga aaggcgccac acatacgaat
    20221 ccaccaacca aagatacggt tggtaaagag ttcttttttt gccataaaga tgaactgttt
    20281 tggcttggtc gcaaaggcca tataaacagg atcccaccag gtacggtgag gcgcaactaa
    20341 aatataattt tcatcttgat taggaatttt atcagtatta tgatagtgag cattgccatt
    20401 gatggaccat aagagcaata caactaatcc acgcaaataa gtataaaaca tgcgatctcc
    20461 ttcaattgtt ttcttgttat tattatacct tatcaaagga gggctggcaa acttttccct
    20521 tgactagatg catatttggg atgaaattag aattctgtta aaaaaaatga tataatagaa
    20581 tttatggata aaaataagat tatgggatta acccaaagag aagtcaagga aagacaggct
    20641 gagggtttgg tcaatgactt taccgcatca gccagtacca gcacttggca aatcgttaaa
    20701 cgaaatgtct ttaccctttt taacgctttg aactttgcca ttgctttggc ccttgccttt
    20761 gtgcaggctt ggagcaatct ggtcttcttt gctgttatct gctttaacgc tttttctggg
    20821 attgtgaccg agctacgagc caaacacatg gtggacaagc tcaatctcat gaccaaggaa
    20881 aaggtcaaaa ccatccgtga tggtcaggaa gttgctctta atcctgaaga attagtgcta
    20941 ggagatgtca ttcgtttgtc tgcaggagag cagattccta gtgatgcctt ggttttggaa
    21001 ggctttgcgg aagtcaatga agccatgtta acgggagaaa gtgatttggt gcaaaaggaa
    21061 gttgacggct tacttttgtc aggaagtttc ctagccagtg ggtcagtttt atctcaagtt
    21121 caccatgtcg gtgcagacaa ctatgctgcc aaactcatgc ttgaggctaa gaccgttaaa
    21181 ctcatcaact cccgtatcat gaaatcgctg gacaagttgg ctggttttac tgggaagatt
    21241 atcattccct ttggtctggc tctcttgctg gaagccttgc ttttaaaagg cctgcctctc
    21301 aagtcatctg ttgtaaactc gtcgacagct cttttgggaa tgttgcctaa gggaattgcc
    21361 cttttgacca ttacttcgct cttgactgca gtgattaagc taggtttgaa aaaggtcttg
    21421 gtgcaggaga tgtactctgt tgagaccttg gcgcgcgtgg atatgctctg tctggacaag
    21481 acgggtacca tcacccaagg aaagatgcag gtggaggctg ttcttccact gacggaaact
    21541 tatggtgaag aggctgttgc cagcatcctg accagctata tggcccatag tgaggataaa
    21601 aatccaacag ctcaagccat tcgccagcgt tttgtgggag atgttgctta tcctatgatt
    21661 tccaatcttc ccttctcgag cgaccgcaag tggggggcta tggagttaga aggtttggga
    21721 atagttttct taggggcacc tgagatgttg ctggattctg aagtcccaga agccagggag
    21781 gccttggaga gaggatcacg tgtcttggtc ttagctctca gtcaggagaa attagaccat
    21841 cacaaaccac agaaaccatc tgatattcag gctctagcct tgctggaaat cttggacccc
    21901 attcgagagg gagcagcaga gacgctggac tatctccgtt ctcaggaggt gggactcaag
    21961 attatctctg gtgacaatcc agttacggtg tccagcattg cccagaaggc tggttttgcg
    22021 gactatcaca actatgtaga ttgctcaaaa atcaccgatg aggaattgat ggccatggcg
    22081 gaggagacag cgattttcgg acgtgtttcc cctcatcaaa agaaactcat catccaaacg
    22141 ttgaaaaaag cgggtcatac aacggctatg acaggggacg gggttaatga tatcttggcc
    22201 cttcgtgagg cggattgttc tatcgtgatg gcggaggggg atccagcaac ccgtcagatt
    22261 gccaatctgg ttctcttaaa ctcagacttt aatgatgttc ctgagattct cttcgagggt
    22321 cgtcgcgtgg tcaataacat tgcccacatc gccccgattt tcttgataaa gaccatctat
    22381 tccttcctgt tagcagtcat ctgtattgcc agtgctttac taggtcggtc agagtggatt
    22441 ttgattttcc ccttcattcc gatccagatt accatgattg accagtttgt ggaaggtttc
    22501 ccaccattcg ttttgacttt tgagcgaaat atcaaacctg tcgagccaaa cttcctcaga
    22561 agatccatgc ttcgtgccct accaagtgcc ctcatggtcg tgtttagcgt tctttttgtg
    22621 aaaatctttg gaacgagtca aggttggtct gagttagaaa tctcaactct cctctattat
    22681 ctcttggggt caattggttt cttatccgta tttagagcct gcatgccatt cactctatgg
    22741 cgagtcctct tgattgtctg gtcagtagga ggcttcctag ccacagctct cttcccaaga
    22801 atccaaaaac tgcttgaaat ttcaaccttg acagaacaaa cgttgcctgt ttatggtgtc
    22861 atgatgttgg tctttacagt gattttcatc ctgactagtc gctatcaagc tagaaaataa
    22921 agaaagactg caatctgtgg attgcagttt ttttaggtac aagattgcca gccgaaatgt
    22981 ggtataataa gaggtaatag agttttggaa agtgagagaa gatgatttca aagagattag
    23041 aattggtagc ttcctttgtg tcacaggggg ctattttact agatgtggga agtgaccatg
    23101 cttatctgcc tatcgagttg gttgagagag gccaaatcaa aagcgctatt gcaggtgagg
    23161 tggtggaagg tccctatcag tctgcggtta aaaatgttga ggctcacggc ctaaaggaga
    23221 aaatccaagt ccgtttagcc aatggcttgg cagcttttga agagactgac caagtgtctg
    23281 tcattaccat tgctggcatg ggtggtcgtt tgattgctag gattttagaa gaaggtttgg
    23341 ggaagttagc taatgtagaa cgtttgatcc tccagcccaa taatcgtgaa gacgacttgc
    23401 gtatctggct acaggatcat ggattccaga ttgtagcaga aagcatctta gaagaagctg
    23461 gaaagtttta tgagattttg gtggtggaag caggacaaat gaagctatca gccagtgatg
    23521 ttcgctttgg tcccttcttg tccaaagaag tcagtccagt atttgtccaa aaatggcaaa
    23581 aagaagctga gaagctagag ttcgccctcg gacaaatccc agaaaaaaat ctggaagaac
    23641 gtcaagttct agtagataag attcaagcta tcaaggaggt gctccatgtt agcaagtgaa
    23701 gtgatccaag cttatgaagc cttttgccct caggaatttt ctatggaggg agacagctgt
    23761 ggtctgcaaa ttggcacttt agacaagggt atccaaaggg tcatggtggc tctcgatatt
    23821 cgtgaagaga cggtggctga agccattgaa aagggtgtag acttgattat cgtcaagcac
    23881 gcgcctatct ttcgtcctat caaggacttg cttgccagcc gtccgcaaaa tcagatttac
    23941 atcgacctga ttaagcatga catcgcagtt tatgtcagcc ataccaatat tgatatcgtt
    24001 gaaaatgggc tcaatgactg gttttgtcag atgctaggaa tcgaggagac gacttatctg
    24061 caggaaacag gtccagaacg tggaattgga cgtattggga atattcagcc tcagacattt
    24121 tgggaattgg cccaacaggt caagcaagtc tttgacctag atagccttcg aatggtgcat
    24181 tatcaagagg atgatttgca gaagcctatt tcaagagtag caatttgtgg tggaagcggg
    24241 cagtctttct ataaggatgc tttggcaaag ggggcagatg tctatatcac tggtgatatc
    24301 tactaccata ctgctcagga tatgctgtct gatggcttgt tagcattgga tccaggtcac
    24361 tatatcgaag tgctttttgt ggaaaaaatc gcaacactcc ttactcaatg gaaagagaaa
    24421 aaaggctggg aactagagat tttacctagt caagcatcga ccaatccttt ccgtcatatc
    24481 tagttagaaa gtgaagacaa tgaaaaaagt tgccattatt ggagcaggaa ttgtgggagc
    24541 aacagctgcc tactacctct cgagagaaag tgacctagag gtgaccgttt ttgaccatga
    24601 gcaaggtcaa gccaccaagg ccgcagcagg aattatcagt ccttggtttt ccaaacgccg
    24661 taataaagcc tggtacaaga tggcgcgctt gggggctgat ttttatgtgg atttattagc
    24721 tgatttagag aaatcaggac aagaaatcga cttttaccag cgttcgggag tctttctctt
    24781 gaaaaaggat gaatccaatt tggaagaact ttatcaactg gccctccagc gcagagaaga
    24841 atctcccttg atagggcaat tagccattct gaaccaagcc tcagctaatg aattattccc
    24901 tggtttgcag ggatttgacc gcctgctcta tgcttctggt ggagcgagag tagatggtca
    24961 cctcttagta actcgtttgc tggaagccag tcaggtcaag ttggtcaaag aaaaagtgac
    25021 tctgacactc ttatcatcag gctaccagat tggcgaagag gtgtttgatc aggttatttt
    25081 ggcaacggga gcttggttgg gggacatgtt agagccttta ggttatgaag tggatgtccg
    25141 tcctcaaaaa ggacaactac gagattatca gcttgcccaa gacatggaag attaccctgt
    25201 tgtcatgcca gaaggggagt gggatttgat tccctttgca ggtgggaaat tatccttggg
    25261 cgctacccat gaaaatgaca tgggatttga tttgacggta gatgaaccct tgctccaaca
    25321 aatggaggag gcggccttgc ctcattaccc agttttagct agagtgactt caagggctga
    25381 gcgtgtggga atccgtgcct ataccagtga tttctctccc ttctttgggc aggtgccaga
    25441 attggcaggt gtctatgcag ccagtggact aggttcatca ggcctcacaa ctggtcctat
    25501 cattggttac catctagccc aactgatcca agacaaggag ttgaccttgg accctgaaaa
    25561 ttacccaatt gaaaactatg tcaaacgagt aaaaagcgaa taatactcaa tgaaaatcaa
    25621 agaacaaact aggaaactag ccgcgggtta ctcaaagcac tgctttgagg ttgtggatag
    25681 aactgacgaa gtcagctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag
    25741 taaccatacc catggcaagg cgacgttgac gtggtttgaa ttggattttc gaagagtata
    25801 agcattttac tgaaatttta tcaggtagtt taggatggca aatgacattc cctgtcaaaa
    25861 atggtaaaat aagaaaaaat gatctgagaa tcgaggaaaa aagatgcaag aaaagatttt
    25921 ggtaactggt ggtgccggtt ttattggaac ccacactgtt attgaattga tccaagcagg
    25981 ccatcaagtt gttgtggtgg ataatcttgt caatagcaat cgtaagagtt tagaagttgt
    26041 tgaaagaatc acaggagttg agattccttt ctatgaggca gatattcgtg acacagatac
    26101 cctcagagat attttcaagc aggaagaacc gactggtgtc attcactttg ctggtttgaa
    26161 ggctgttggc gaatcaacac gtatccctct tgcctactat gacaacaata tcgctggaac
    26221 tgtcagcctt ttgaaagcca tggaagaaaa caactgtaaa aacatcatct tcagttcttc
    26281 tgcgacagtt tacggagatc cgcacacagt gcccatcttg gaagatttcc cactttcagt
    26341 gacaaaccca tacggtcgta ccaagctcat gctagaggaa attttgaccg atatttacaa
    26401 agcagactca gaatggaatg ttgtcttgct tcgttacttt aacccaatcg gagcccatga
    26461 gagtggtgat ttgggagaaa atccaaacgg tattccaaac aatctcttgc catatgtgac
    26521 tcaagtagcc gttggaaaat tagagcaagt gcaagtgttt ggagacgatt acgatacgga
    26581 agatggaaca ggtgttcgtg actatatcca cgttgtcgat ttggctaagg gtcacgttgc
    26641 agctttgaaa aaaatccaaa aaggttcagg actaaacgtt tataaccttg gaactggtaa
    26701 aggttactca gttcttgaaa ttatccaaaa catggaaaaa gcggtgggac gtcctattcc
    26761 ttaccgcatc gtagaacgtc gcccaggtga tatcgctgcc tgctactcag acccagcaaa
    26821 agctaaagca gaactcggtt gggaagcaga actcgacatc acccaaatgt gtgaagacgc
    26881 atggcgttgg caaagcaagc atccaaatgg atttgaagac taagatgatg atttcaatca
    26941 tcgtcccttg tttaaacgaa gaggaagtac ttcctctttt ttatcaggct ctggaagctt
    27001 tacttccaga tttggaaaca gaaatcgagt atgtctttgt cgatgatgga tcaagtgatg
    27061 ggaccttgga actcttaaag gcctatcggg agcaaaatcc ggcagtccat tatatttctt
    27121 tctctcgaaa ttttggcaaa gaagcagccc tttatgcagg cttgcaatat gcgacaggag
    27181 atttggtggt ggtgatggat gcagacctcc aagatcctcc tagtatgttg tttgagatga
    27241 aaaatgtact agacaaaaat gtagacttgg actgcgttgg gacacggaga actagtcggg
    27301 agggagaacc cttctttcgc agtttctgtg ctgttctctt ttatcgcctc atgcaaaaaa
    27361 tcagcccagt agctctgccg tcgggtgtcc gtgattttcg tatgatgaga aggtctgtag
    27421 tcgatgccat tttaagcttg accgagtcca atcgtttttc aaaggggctc ttcgcctggg
    27481 tgggtttcaa aacctactat ttggactatc caaatgtcga aaggcaggct ggcaagacaa
    27541 gttggagttt taggcaactc tttttctact ccattgaagg gattattaat ttttcagatt
    27601 ttcccttgat tatagccttt gtagctggtc tcctatcttg ttttctttct ctgctgatga
    27661 ccttttttgt tgtggttcgg accctcattt tgggcaatcc gacatctggt tggacctctc
    27721 tgatggctgt tattctcttt cttggaggca ttcaactctt gaccattggg attctcggca
    27781 agtatatcag taagatttat ttagagacta aaaaaagacc actttatctt atcaaagaaa
    27841 aaagtgacct tcctgatttt acagaaaaaa ataaagtgaa aagactataa ttttacatgg
    27901 aaatgtgcta aactagaggg agtggattat cgcccttgat ttaggcttcc tggtttccaa
    27961 tcagggcgcg agttgggcaa tttttaacag cctctaagac atcctgacta ggagaaattt
    28021 ctttttccag ttggtcaggg tcatcgtaaa aacgcacgat tccattatcg tggtaatcaa
    28081 ataaatcaga ataagtttgg caaagcccac aggcgataca tcgttcaggt ataagtgtga
    28141 ttttcatgtt catatttata ttgtaataag aaaagtaaaa aaaaacaagg agtaaggtat
    28201 ggcaaaagaa ccgtggcaag aagatatcta tgatcaagaa gaatcaagag cagagcgtcg
    28261 gcatcgaaac cacggagggg ctgataggat ggctaatcgt attttgacga tcctagctag
    28321 tattttcttt gtaattgtgg tggtgatggt catcgttctc atctatctat catcgggggg
    28381 aagtaatcgc acagcagcct taaaagactt tcatgattct gatgcaagtg tagtacaaat
    28441 ctcatcttca agcagttctc atcctgagca gagttcagag ccagaatcta cttctagtag
    28501 ttcagaagaa gctgctaatc ctgaaggaac gattaaagtt ctcgcaggag aaggggaagc
    28561 agctattgcc gctcgtgcag gaatctccat tgctcagtta gaggccttga atcctgggca
    28621 catggctaca ggatcttggt ttgctaatcc aggtgatgtt ataaaaataa aataggagcc
    28681 gatatgaaaa caattcaaat tgctattgat ggtcctgctt ccagcggtaa gagtacggtc
    28741 gcaaagatta ttgctaagga ttttggattc acctaccttg atacaggagc tatgtatcgt
    28801 gcagcgacct atatggctct taagaaccaa ttaggagttg aagaagtcga agcccttcta
    28861 gccttgttgg accagcatcc aatcagcttt ggacgttcag aaactggaga ccagcttgtt
    28921 tttgtaggag atgtggatat tacccatcct atccgtgaaa atgaagtgac caatcatgtt
    28981 tctgctattg cagcaattcc tgaagtgcgt gagaaactgg tttctctcca acaagaaatt
    29041 gcccagcaag gcgggattgt catggatggt cgcgatattg gaactgttgt attgccacaa
    29101 gcagaattga aaattttcct agtagcttct gttgatgaga gagcagagcg tcgttacaag
    29161 gaaaatattg ccaagggaat tgaaacagac cttgaaaccc taaaaaagga aattgctgcg
    29221 cgtgactaca aggatagtca tcgtgagact tctcctctca aacaagcaga ggatgctgtc
    29281 taccttgata caactggttt gaacattcaa gaagtagttg aaaaaatcaa agcagaagct
    29341 gaaaaaagaa tgtaaatgta gaaaagccga tagattgata tcggtttttt tctaaatttg
    29401 tcaaaatgct gattttaggg tataatagtt gctgttcgag aaattttgat tttcaaagaa
    29461 tagtgaatga tgataaggag aaaccatgaa caatttacca aattgtccaa aatgtaactc
    29521 agagtatgtc tacgaagacg gtgccctact ggtttgccca gagtgtgctc atgagtggaa
    29581 tcctgctgaa gttgcagaag tagaagaagg tcttgtcgct atcgatgcca acggaaataa
    29641 attgactgat ggtgatacag taactctcat caaggacttg aaagtaaaag gtgcgccaaa
    29701 agatttgaaa caagggacgc gcgtgaaaaa tatccgcatc gtagaaggcg atcacaatat
    29761 cgactgtaag attgatggtt ttggtgccat gaaacttaaa tcagagtttg tgaggaagat
    29821 ttaagcatgt caaaacacta taaacttgta ttttatagcc gtatcttctt gtttctagcg
    29881 gcttttacgg gagtttatct tgaaattact aagcatggtg gttttgggat gcttctctat
    29941 tacacggttc tgtccaacct cttggtaact atttttaccc tttatcttct aaaggttatg
    30001 agccgtgtag gtgaaaattg gcaaaggcca agtctcttgc gcttaaaagg tggggtcacc
    30061 atgagtatca tgattacctg tgtgatttac catttcctct tggcgcccat tgcgactaat
    30121 ttctataccc tagaaaattt cctttgccac tatatcgttc ccatctggtt tttagcggat
    30181 accctctttt ttgacaaaca gggtcaatac aagatttggg atccagcagt gtggaccatt
    30241 ttaccctttc tgtatatgat gtttgctctt tttaatggct tggttctaaa actcaatatt
    30301 ccaaatgcca aggataatcc tttcccttac ttctttttga atgtgaacaa gggttggaat
    30361 gttgtgttta agtggtgtct gattatcttt gttgcctata tggtagcagg atttattttc
    30421 tactttatca agcaaatcaa gagaaagtca tcctaatact cttcgaaaat ctcttcaaac
    30481 cacgttagtt tccatctgca acctcaaaac actgttttga acaacctgcg gctagctttc
    30541 tagtttgctc tttgattttc attgagtatg agatacaagg gattcatttt cgaatttaag
    30601 aaggagtcta caaaccaatg aggcttcttc ttttcttgct tatttgataa ctatcaaaaa
    30661 agtagaagat taagagaaat atagaaagag atttcatgaa acaattttta gaacgggcca
    30721 gcattttggc tctctccctc gttttgatta cctccttttc catttcgagt gccctaccag
    30781 ccatgtttga ctattatcag ggttattcta aggaacaaat tgagctcttg gtgagcttgc
    30841 cttcctttgg aatcatgatg atgttactgc taaatggttt cttagaaaaa atatttcctg
    30901 agcgcttaca gattagtttg ggcttgctga ttttatcatt gagcggtaca gctcccttct
    30961 ggtaccaagc ctatcccttt gtctttggaa cacggcttct ctttggtttg ggtcttggga
    31021 tgatcaatgc caaggccatt tctattatca gtgaacgcta ccaaggaaaa aggcgaattc
    31081 agatgttagg gctacgcgct tctgcagagg tcgttggagc ttctctcatt accttggccg
    31141 tcggtcagtt gttggccttt ggttggacag ctatctttct agcctatagt gctggatttt
    31201 tggtgatgcc cctttatctg ctctttgtcc cttatggaaa atcaaagaaa gaagtcaaga
    31261 aaagagcgaa ggaagcaagt cgtttaactc gagaaatgaa aggcttgatt tttaccttag
    31321 ctatcgaagc ggcagttgta gtttgtacca atacagctat taccatccgt attccaagtt
    31381 tgatggtgga aagaggattg ggggatgccc agttatctag ttttgttctt agtatcatgc
    31441 agttgatcgg gattgtggct ggggtgagtt tttctttctt gatttctatc tttaaagaga
    31501 aactgctcct ctggtctggt attacctttg gcttggggca aatcgtgatt gccttgtctt
    31561 catccttgtg ggtggtagta gcaggaagtg ttctggctgg atttgcctat agtgtagtct
    31621 tgacgacggt ctttcaactt gtctctgaac gaattccagc taaactcctc aatcaagcaa
    31681 cttcatttgc tgtattaggc tgtagtttcg gagcctttac gaccccattt gttctaggtg
    31741 caattggctt actaactcac aatgggatgt tggtctttag tatcttagga ggttggttga
    31801 ttgtaatctc tatctttgtc atgtacctac ttcagaagag agcttaggat tgattcctaa
    31861 gtttttcttt tgatactcaa tgaaaatcaa agagcatagt tgattgagtt tggaatagta
    31921 cgctgtagct gctaaaatat ttctagaaat taatttgact tttctaatag agttattcat
    31981 atcttatttc aatctactat agaaagctag ccgcaggttg ctcaaaatag tgtgttgagg
    32041 ttgcagatgg aaactgacgt ggtttgaaga gattttcgaa gagtatgaga tttttatacc
    32101 catacaatat ttgttttagg tccttgttta cagactaatt tcttgataaa atagaggtta
    32161 tgacgagata taaagcaact atttcctatg atggttatgc ctttgctggc tttcagcgcc
    32221 agtctcatgc gcgtagcgtt caggaagaaa ttgaaaaaac cttgaccaga ttaaataaag
    32281 ggcaaaccat tactgttcac ggtgctggta ggacagatag tggggttcat gccctgggac
    32341 aggtcattca ttttgacctg ccttatcaga tggatgagga gaaactccgt tttgccttgg
    32401 atacccagtc tcctgaagat attgatgtga tttcgattga gcttgtggca gatgattttc
    32461 attgccgtta tgccaaacat agcaagacct atgagtttat tgtggataga ggacgtccca
    32521 aaaatcctat gcgccgtcac tatgccaccc actttcccta cccactcgat gtggaacgaa
    32581 tgcagattgc aatcaaaaag ctagagggaa cccatgattt taccggtttt acagcctctg
    32641 ggactagtgt agaggataag gttcgcacca tcacagaagc tagtttaata gtcgatgaga
    32701 caggacaatt tttgaccttt accttttcag gaaatggttt cttgtataaa cagattcgca
    32761 atatggtggg gactcttctc aaaatcggta acaaccgcat gccagtagag cagattgatc
    32821 tcatcttgga gaagaaggac aggcaacttg caggtcccac tgcagcacca aatggtttgt
    32881 atttaaagga gattcgttat gaagaataat cgtattttag cactttctgg aaatgatatt
    32941 tttagtggtg gtggactgtc agctgatttg gctacctata ccttgaacgg cttgcatggg
    33001 tttgtagcag tgacttgttt gacagccttg acagaaaaag gatttgaagt ctttccaact
    33061 gatgatacca tttttcaaca tgaattagat agcttacgtg atgtggaatt tgggggaatt
    33121 aagattggtc ttctccctac tgtcagtgtg gctgagaagg ccttggactt tatcaaacaa
    33181 cgcccaggag tacctgtggt gttggatcct gtcttggtct gcaaggaaac gcatgatgta
    33241 gctgtcagtg agctctgcca agagttgatt cgctttttcc cttatgtcag tgtgattacg
    33301 cctaatctcc cagaagcaga attattatcc ggtcaggaaa ttaaaacctt ggaagacatg
    33361 aaaactgcag cgcagaaatt gcatgattta ggagcgccag cagtcattat caagggaggc
    33421 aatcgtctta gtcaggacaa ggctgtggat gtcttttatg atggacagac ctttactatc
    33481 ctagaaaatc cagttatcca aggccaaaat gctggtgcag gttgtacctt tgcctctagc
    33541 attgccagtc acttgattaa aggtgataaa cttttgccag cagtagaaag ctctaaggct
    33601 ttcgtttatc gtgctattgc acaagcagat cagtatggag taagacaata tgaagcaaac
    33661 aaaaacaact aaaatcgccc ttgtatccct attaaccgcc ctttctgtgg ttctaggtta
    33721 tttcttaaaa atcccaacac ctacaggaat tctaactctt ttagatgctg gtgtcttctt
    33781 tgcggccttt tactttggta gtcgtgaagg agcggtagtc ggaggactag caagtttctt
    33841 gattgacctc ttatcaggct accctcagtg gatgttcttt agcttggtca accatggctt
    33901 gcagggattt ttcgcaggat ttaaaggaaa aagtcagtgg ttaggcctta ttttagcaac
    33961 tattgccatg gtaggaggct acgccttggg ttctactttg atgaatggct gggcagcagc
    34021 cctcccagaa attctaccaa atttcatgca aaatatggta gggatgattg taggatttat
    34081 tcttagtcaa agtatcaaga agattaagta atactcttcg aaaatctctt caaaccacgt
    34141 cagcttccat ctgcaacctc aaaacactgt tttgagcagc ctgaggctag ctttctagtt
    34201 tgcactttga ttttcattga gtataaagag gttgagtaaa aagtatttca aataagaaaa
    34261 acgcatcata tcaggtgttg aatgaactac ccccc
//
LOCUS       DAYYQN010000018        31620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYYQN010000018 DAYYQN010000000
VERSION     DAYYQN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.018"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(546..2804)
                     /gene="glgP"
                     /locus_tag="ACHBRQ_000783"
     CDS             complement(546..2804)
                     /gene="glgP"
                     /locus_tag="ACHBRQ_000783"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HFM9592489.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2830..4362)
                     /gene="malQ"
                     /locus_tag="ACHBRQ_000784"
                     /pseudo
     CDS             complement(2830..4362)
                     /gene="malQ"
                     /locus_tag="ACHBRQ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            4897..6168
                     /locus_tag="ACHBRQ_000785"
     CDS             4897..6168
                     /locus_tag="ACHBRQ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9592490.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6279..7571
                     /locus_tag="ACHBRQ_000786"
     CDS             6279..7571
                     /locus_tag="ACHBRQ_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9592491.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7573..8415
                     /locus_tag="ACHBRQ_000787"
     CDS             7573..8415
                     /locus_tag="ACHBRQ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9592492.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8668..9468
                     /locus_tag="ACHBRQ_000788"
     CDS             8668..9468
                     /locus_tag="ACHBRQ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9592493.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9478..10464
                     /locus_tag="ACHBRQ_000789"
     CDS             9478..10464
                     /locus_tag="ACHBRQ_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9592494.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10606..10809
                     /locus_tag="ACHBRQ_000790"
     CDS             10606..10809
                     /locus_tag="ACHBRQ_000790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592495.1"
                     /translation="MKIKVQTRKLAAGCSKHGFEVADRTDEVSSKHGFEVVDETDEVS
                     SKHGFEVADRIDEVSNIYTARRR"
     gene            complement(10845..11786)
                     /locus_tag="ACHBRQ_000791"
     CDS             complement(10845..11786)
                     /locus_tag="ACHBRQ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9592496.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11764..13527)
                     /gene="aspS"
                     /locus_tag="ACHBRQ_000792"
     CDS             complement(11764..13527)
                     /gene="aspS"
                     /locus_tag="ACHBRQ_000792"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9592497.1"
                     /translation="MKRSMYAGRVREEHIGQEIALKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(13646..13882)
                     /locus_tag="ACHBRQ_000793"
     CDS             complement(13646..13882)
                     /locus_tag="ACHBRQ_000793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592498.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSSKHGFEVVDETDEVS
                     SKHGFEVVDETDEVSNHTYGKATLTWFEEIFEEY"
     gene            complement(13924..14154)
                     /locus_tag="ACHBRQ_000794"
     CDS             complement(13924..14154)
                     /locus_tag="ACHBRQ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592499.1"
                     /translation="MKRVILVAVIQAVILFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(14172..14813)
                     /locus_tag="ACHBRQ_000795"
     CDS             complement(14172..14813)
                     /locus_tag="ACHBRQ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9592500.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(14825..15558)
                     /locus_tag="ACHBRQ_000796"
                     /pseudo
     CDS             complement(14825..15558)
                     /locus_tag="ACHBRQ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
     gene            complement(15561..15758)
                     /locus_tag="ACHBRQ_000797"
     CDS             complement(15561..15758)
                     /locus_tag="ACHBRQ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592501.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(15758..15964)
                     /locus_tag="ACHBRQ_000798"
     CDS             complement(15758..15964)
                     /locus_tag="ACHBRQ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9592502.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(15974..16321)
                     /locus_tag="ACHBRQ_000799"
     CDS             complement(15974..16321)
                     /locus_tag="ACHBRQ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592503.1"
                     /translation="MIDKVVRNLLLTFFLCKMTKIINFLTTILVKKKKMCYNVSKLRE
                     KKKGNAEMSRLLQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQ
                     RIEDIQTVEFDGE"
     gene            complement(16321..16467)
                     /locus_tag="ACHBRQ_000800"
     CDS             complement(16321..16467)
                     /locus_tag="ACHBRQ_000800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592504.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKVKLTWFEEI
                     LEEY"
     gene            complement(16464..17753)
                     /gene="hisS"
                     /locus_tag="ACHBRQ_000801"
     CDS             complement(16464..17753)
                     /gene="hisS"
                     /locus_tag="ACHBRQ_000801"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9592505.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(17831..19078)
                     /locus_tag="ACHBRQ_000802"
     CDS             complement(17831..19078)
                     /locus_tag="ACHBRQ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFM9592506.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSAFILFLGKSKLSLSISSADGEKLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQVCVLLAIIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(19269..20120)
                     /gene="rgg"
                     /locus_tag="ACHBRQ_000803"
     CDS             complement(19269..20120)
                     /gene="rgg"
                     /locus_tag="ACHBRQ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HFM9592507.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRKTPDS"
     gene            20244..20585
                     /locus_tag="ACHBRQ_000804"
     CDS             20244..20585
                     /locus_tag="ACHBRQ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9592508.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            20649..20948
                     /locus_tag="ACHBRQ_000805"
     CDS             20649..20948
                     /locus_tag="ACHBRQ_000805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592509.1"
                     /translation="MKIKGQTRKLAAGCSKHCFEVMDRTDEVSSKYCFEVVDRTDEVS
                     SKHCFEVVDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHTYGKATLT"
     gene            complement(20974..22677)
                     /gene="ilvD"
                     /locus_tag="ACHBRQ_000806"
     CDS             complement(20974..22677)
                     /gene="ilvD"
                     /locus_tag="ACHBRQ_000806"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFM9592510.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(22910..23842)
                     /locus_tag="ACHBRQ_000807"
     CDS             complement(22910..23842)
                     /locus_tag="ACHBRQ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFM9592511.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(23839..24696)
                     /locus_tag="ACHBRQ_000808"
     CDS             complement(23839..24696)
                     /locus_tag="ACHBRQ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9592512.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRLTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(24700..26046)
                     /locus_tag="ACHBRQ_000809"
     CDS             complement(24700..26046)
                     /locus_tag="ACHBRQ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9592513.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(26059..26343)
                     /locus_tag="ACHBRQ_000810"
     CDS             complement(26059..26343)
                     /locus_tag="ACHBRQ_000810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9592514.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(26347..28377)
                     /locus_tag="ACHBRQ_000811"
     CDS             complement(26347..28377)
                     /locus_tag="ACHBRQ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9592515.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            28569..29576
                     /locus_tag="ACHBRQ_000812"
     CDS             28569..29576
                     /locus_tag="ACHBRQ_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9592516.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQNRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            29579..29779
                     /locus_tag="ACHBRQ_000813"
     CDS             29579..29779
                     /locus_tag="ACHBRQ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9592517.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            29931..30113
                     /gene="rpmF"
                     /locus_tag="ACHBRQ_000814"
     CDS             29931..30113
                     /gene="rpmF"
                     /locus_tag="ACHBRQ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9592518.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            30129..30278
                     /gene="rpmG"
                     /locus_tag="ACHBRQ_000815"
     CDS             30129..30278
                     /gene="rpmG"
                     /locus_tag="ACHBRQ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9592519.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(30758..>31620)
                     /locus_tag="ACHBRQ_000816"
     CDS             complement(30758..>31620)
                     /locus_tag="ACHBRQ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HFM9592520.1"
                     /translation="KHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKST
                     EFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKN
                     SNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYL
                     NESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWY
                     YLNESGSMATGWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGY
                     RVNANGEWVG"
BASE COUNT         9821 a         6769 c         5646 g         9384 t
ORIGIN      
        1 aaaagaatgt agggtttatt tccctacatt cttaaaataa cttagtacat aaatgacaat
       61 gctgccactg ccgtcaatat tgcaaaaact attttccaaa tttttttatt ttttccaaaa
      121 atcttatcag gtaacaagct tattacaaga aaggtaataa acaacaccaa tttaagtgtg
      181 tatatcataa aaacaccttc tttcctacaa ttatattata atactttatg taagcgaata
      241 caagagatgt gaggtaacca actacgaaaa aatataaggc tgaagacagt aaaaaccagc
      301 atttaaagcc ttcactgtat ctaggatttc cctattttta gacaagattg acccaaatcc
      361 acagcattta aaaaagcgaa tttcaatcga aactcgcttt tttacaaaat gtattagtat
      421 aaatttgtat cttatactca atgaaaatca aagagcaaac taggaagcta gccgcaggtt
      481 gctcaaagca ccgctttgag gttgcagata aagctgacgt ggtttgaaga gattttcgaa
      541 gagtattagt tcaagtgcca gatgtcttca ttatactgag cgattgtacg gtcagatgag
      601 aagaatcctg ctttagaaat gttaacgatg actttatcca accatgcgtc acggtcttcg
      661 tagtcagcaa gcatttgctc tttgactttg atgtagtctt ccaagtcaag aagagtcatg
      721 aaccagtctt tgttgatcaa ttcattgtaa agacgttcca agcgctcttt gtttccagct
      781 gcaagaactg catcactaac gatgaagtca accaatggtt tgatagcttc acgagcgtag
      841 aattcgcttg atttgtaagc tgcttttgcg taaaggtcga taacagtttc tgaatcttca
      901 ccgaagatgt agatgttttc ttctccaacc aactcagcga tttccacgtt agcaccgtcc
      961 atagtaccaa gtgtcaaagc tccgttcaac atgaatttca tgttaccagt acctgaagct
     1021 tctttagaag caagtgaaat ttgttctgag atatcacatg ctgggataag gaaacttgct
     1081 gcagtaacgt tgtagttttc aaccataact acttgcaagt gtggagctac tgctggatcg
     1141 ttagcaataa cttctgacat gcaaaggatt aaatggataa tgtcttgagc gattgtgtag
     1201 gctggagctg ctttaccacc aaagaagatt gtgattggac gagcagggat gttaccagct
     1261 ttgatgtcaa ggtatttgtg gatcacgtac aaagcgttca tttgttggcg tttgtactcg
     1321 tgaagacgtt tgatttggat atcaaagata gaatttgaat tgatttccac accttggtgt
     1381 tctttcaagt gacgagccaa tttacgtttg ttgtgagcct tgatgctttc caatttttct
     1441 ttgacagctg ctttgtcttc ataagacaaa agtttttcaa gctcatctgc ttcatggtgc
     1501 caaccatctc caagaatctc atctaagtag tgagacaatc ttgggttagc atgcataagc
     1561 caacgacgga aagtgatacc gtttgttttg ttgttgaact tttctgggta aaggtcgtag
     1621 aaggctttca actcagaatt tttcaagatt tcagtatgga gtgctgcaac cccgttaaca
     1681 ctgtatccgt agtggatatc catgtgagcc atgtgaacac gtccgctctc atcgatgatt
     1741 tgaacagctg gatctttgta ctctgccttc acacgacggt ccaattcttc gatgattggt
     1801 accaagtgag gaaccacttc ttgcaagaat tcaagaggcc atttttcaag cgcttcagca
     1861 aggattgtgt ggttagtgta ggcagtcatg ctacgaacaa ttgagattgc ttcgtcaagc
     1921 tcgataccac gtgcagttaa aagacgaatc aattcaggaa tcaccattga tgggtgagta
     1981 tcgttgattt ggacaactgc gtagtcagca aggtcatgca agttgcttcc tttttcgatt
     2041 gcttcgtcga tgatcaattg cgcaccgttt gaaaccatga agtattgttg gaagatacgg
     2101 agcaattcac cttgacggtc actatcatct gggtaaagga agagagtcaa gttgcgagcg
     2161 atatctgtct tgtcaaagtt gataccatct ttaataatag aagaatcaac tgaatccaag
     2221 tcaaacaaac gcaagcggtt tttagtcgct gtttcataac cagtaacatc aatatcgtaa
     2281 agagttgatg tcaaagtaaa gtctgcaaat ggtacttggt agctacggct tgagcgaacc
     2341 aaccagtttt gctctgtcaa ccatgcattt ggaattgttt cttgttggtt gtttttaaga
     2401 acttgttgga aaagaccaaa gtggtagtta agaccaacac cgtcaccatt caaaccaaga
     2461 gtagcaattg agtcgataaa gcaggcagcc aaacgtccca aaccaccatt accaagagat
     2521 ggttccaatt caacttcttc aacttcgatc aagtctttac ctgcagctgc aagttctttt
     2581 ttaacatcgt cgtaaagacc aaggttaatc aagttgtttg acaagagttt accaatcaag
     2641 aactcagctg agatgtagta aacttttttc ttaccagtgt tgactggttt ttggctgctt
     2701 gcaagcttgc tgtagttaag aagagcaagg taaagctctt cattgctaca ttctgcaatg
     2761 gttttattgt aacgattttg tacaaattct tgtagtgata acatgtttag ttgtctcctg
     2821 attattgtct tatttcttta aatctaccaa attttcatta attcggcgat aaattgttgt
     2881 caagtcaagc aaaccttcct cgacagctgg tgtcaattga tcttcagtca tacgccaaga
     2941 ccagtttcca ccaagggtag atgggaagtt catacgagct gcctcatcca attctagtaa
     3001 atcttgcata gttgcaattg ccataaagct aactgatgaa aatactgtac gaagcatagc
     3061 gtgtaccact gtttcgtatt ctttacggtt cgtgtaacga gccatgtact cacgagtcgc
     3121 atcatcaatc tcattacggt accaaccaag aaccgtattg ttatcgtgtg ttcctgtgta
     3181 cataactgag ttagcaggtg ccaagtgtgg gctatcaatg ctttcgtctt ctgggttgaa
     3241 ggcaaattga agaatcttca ttcctgggaa gccagtacgt tcacgtaatt cgatcacttc
     3301 atctgtcatg aagccaaggt cttctgcgat gatgtttagc tcaccaagtt cttccttaac
     3361 ggctgcaaaa agcttgtagc ctggaccttt cacccactca ccaggtgctg ctgtatcgga
     3421 accagcaggg atttcccaat aagattcgaa gccacggaag tggtcgatac gaacgatatc
     3481 gtagattttg aagctttcac gcaagcgttc aatccaccat ttgtagccgt ctttgtccat
     3541 tgcttcccag tcatagattg ggttacccca aagctgacca gttacagaaa actcatctgg
     3601 tgggcatcct gcgatacaag tagccttacc attgacatct gttttgaaga gatgtggatt
     3661 tgcccacata tcacttgaat cttccgctac gtagattggc atgtccccaa cgatttcaat
     3721 gtggttgtcg ttagcgtaag ctttcaattt caaccattgt tggaagaaga agtattgagt
     3781 cacacggtgg taaaccaact tgtctgccaa ttgctcacga tagctttcaa gtgctgaagc
     3841 tttacgagca cgagcatctg catctggcca ttcagtccaa gcaagattgt caaaatgctc
     3901 tttgatagcc atatactcag caaagagctc aagccatgat tggttgtctt gagcaaattt
     3961 ctcaaaatct ttaacatctc cgacttcaaa gaaacgtttc accgcttttt ctaaaagagg
     4021 acgacgtgca tagtagattt tagcatagtc aacttcagac gcatcgctac caaagtcaac
     4081 tccttcaagg tcactcgctc ccaacaaacc ttgctccacc aagatatcta aatcgataaa
     4141 atgagtgttt cctgcgaagg ctgagaaaga ttggtaagga gaatccccgt aactagttgc
     4201 tcctaatgga aggatttgcc agtaacgttg ttttgtacgg accaagaaat caacgaagtc
     4261 gtaagcactt tgaccaaatg atccgattcc gtaagctcct ggaagagaag agatgtgcat
     4321 caacacacca ctttgacgtt ttttcataat agcacctcgt gtgttaaaat aatggaacgt
     4381 tgcgtatttt gcagacgcaa acgtttgcgt tacttataag tatactcctt ttcaagcata
     4441 attgcaagcg tttttaaaaa acttttttga ttatttttaa ggagactaac tcattttagc
     4501 aaagataact taaaactatc taatcctaaa gtttgactct cacgagacag tctacaaagt
     4561 acaaaacctc cttagggagc ttgaatttat atgcaacaaa gcacaaacgt ctatagaaaa
     4621 tagataggga ttagaaacag ggaggtagcc cctcctgatt tccctcttta gacagattcc
     4681 atatattttt attaaaaata ctgtattctt atctatttca tacttttctt ctattaaaat
     4741 gcaaaattat gctatcaaat caagcttaaa aattttttaa aatttttaca aaaaatactt
     4801 gcaaccgttt tctatttgtg ctatactaag ctcataaagg aaaacgtttg cgtttcctta
     4861 tcaataaaac ttgctattct ttgggaggaa tacactatgt catctaaatt tatgaagagc
     4921 actgcggtgc ttggaactgt tacacttgct agcttgcttt tggtagcttg cggaagcaaa
     4981 actgctgata agcctgctga ttctggttca tctgaagtca aagaactcac tgtatatgta
     5041 gacgagggat ataagagcta tattgaagag gttgctaaag cttatgaaaa agaagctgga
     5101 gtaaaagtca ctcttaaaac tggtgatgct ctaggaggtc ttgataaact ttctcttgac
     5161 aaccaatctg gtaatgtccc tgatgttatg atggctccat acgaccgtgt aggtagcctt
     5221 ggttctgacg gacaactttc agaagtgaaa ttgagcgatg gtgctaaaac agacgacaca
     5281 actaaatctc ttgtaacagc tgctaatggt aaagtttacg gtgctcctgc cgttatcgag
     5341 tcacttgtta tgtactacaa caaagacttg gtgaaagatg ctccaaaaac atttgctgac
     5401 ttggaaaacc ttgctaaaga tagcaaatac gcattcgctg gtgaagatgg taaaactact
     5461 gccttcctag ctgactggac aaacttctac tatacatatg gacttcttgc cggtaacggt
     5521 gcttacgtct ttggccaaaa cggtaaagac gctaaagaca tcggtcttgc aaacgacggt
     5581 tctatcgcag gtatcaacta cgctaaatct tggtacgaaa aatggcctaa aggtatgcaa
     5641 gatacagaag gtgctggaaa cttaatccaa actcaattcc aagaaggtaa aacagctgct
     5701 atcatcgacg gaccttggaa agctcaagcc tttaaagatg ctaaagtaaa ctacggagtt
     5761 gcaactatcc caactcttcc aaatggaaaa gaatatgctg cattcggtgg tggtaaagct
     5821 tgggtcattc ctcaagccgt taagaacctt gaagcttctc aaaaatttgt agacttcctt
     5881 gttgcaactg aacaacaaaa agtattatat gataagacta acgaaatccc agctaatact
     5941 gaggctcgtt catacgctga aggtaaaaac gatgagttga caacagctgt tatcaaacag
     6001 ttcaagaaca ctcaaccact gccaaacatc tctcaaatgt ctgcagtttg ggatccagcg
     6061 aaaaatatgc tctttgatgc tgtaagtggt caaaaagatg ctaaaacagc tgctaacgat
     6121 gctgtaacat tgatcaaaga aacaatcaaa caaaaatttg gtgaataaaa aatttgttca
     6181 aggggggtgg aaatcaaatc cccctttgaa tttatcaata gagacacaaa taatttagct
     6241 ttcttataaa aaagtagtat cctatgaaag gagttaatat ggaaaagcaa caacctagta
     6301 aagcagccct gctgtctatc attcctgggt taggacagat ttacaataaa caaaaagcca
     6361 aaggttttat cttccttggt gtaaccatcg tatttgtcct ttacttccta gcacttgcaa
     6421 cccctgaatt gagcaacctc atcactcttg gtgacaaacc aggtcgtgat aattccctct
     6481 ttatgctgat tcgtggtgcc ttccatctaa tctttgtaat cgtttatgta ctcttttatt
     6541 tctcaaatat caaagatgca catacgattg caaaacgcat taacaatgga attccagttc
     6601 cacgcacact caaagacatg atcaaaggga tttatgaaaa tggcttccct tacctcttga
     6661 tcattccatc ttatgttgcc atgaccttcg cgattatctt cccagttatc gtaaccttga
     6721 tgatcgcctt taccaactac gacttccaac acttgccacc aaacaagttg ttggactggg
     6781 ttggtttgac caactttaca aacatttgga gcttgagtac cttccgttct gcctttggtt
     6841 ctgttctttc ttggactatc atttgggctt tggcagcttc tactttacaa atcgtaattg
     6901 gtatcttcac agctatcatt gccaaccaac catttatcaa aggaaaacgt atctttggtg
     6961 ttattttcct tcttccttgg gctgtcccag ccttcatcac tatcttgaca ttctcaaata
     7021 tgtttaacga tagtgtcggt gctatcaaca ctcaagtatt gccaatcttg gctaaattcc
     7081 ttcctttcct tgatggagct cttattcctt ggaaaacaga cccaacttgg actaagattg
     7141 ccttgattat gatgcaaggt tggctcggat tcccatacat ctacgttttg actttgggta
     7201 tcttgcaatc tattcctaac gacctttacg aagcagctta tattgacggt gccaacgctt
     7261 ggcaaaaatt ccgcaacatc actttcccaa tgattttggc tgttgcggca cctactttga
     7321 ttagccaata caccttcaac tttaacaact tctctatcat gtacctcttc aatggtggag
     7381 gacctggtag tgtcggaggt ggagctggtt caaccgatat cttgatctca tggatctacc
     7441 gtttgacaac aggtacatct cctcaatact caatggcggc agctgttacc ttgattatct
     7501 ctatcattgt catctcaatc tctatgatcg cattcaagaa actacacgca tttgatatgg
     7561 aggacgtcta agatgaataa ctcaattaaa ctcaaacgta gactgactca aagccttact
     7621 tacctttacc tgattggtct atcaattgta attatctatc cactgttgat taccattatg
     7681 tcagccttta aagcaggtaa cgtctcagcc tttaaactag atactaatat cgacctcaat
     7741 tttgataact ttaaaggcct cttcactgaa accttgtacg gtacttggta cctcaacact
     7801 ttgattatcg ccttaattac catggctgtt caaacaagta tcatcgtact tgctggttat
     7861 gcttacagcc gttacaactt cttggctcgt aaacaaagtt tggtcttctt cttgatcatc
     7921 caaatggtgc caactatggc cgctttgaca gccttcttcg ttatggcgct tatgttgaac
     7981 gcccttaacc acaactggtt cctcatcttc ctctacgttg gtggtggtat cccgatgaat
     8041 gcttggctca tgaaaggcta cttcgataca gtgccaatgt ctttagacga atctgcaaaa
     8101 ctagacggtg caggacactt ccgccgcttc tggcaaattg ttctaccact tgttcgccca
     8161 atggttgccg tacaagctct ctgggccttc atgggacctt tcggggacta catcctctct
     8221 agtttcttgc ttcgtgagaa agaatacttt actgttgccg taggtctcca aaccttcgtt
     8281 aacaatgcga aaaacttgaa gattgcctac ttctcagcag gtgctatcct catcgccctt
     8341 ccaatctgta ttctcttctt cttcctacaa aagaactttg tttcaggact tacaagtggt
     8401 ggcgacaagg gataatttat ccccgccacc ctttttcatt ttatactctt cgaaaatctc
     8461 ttcaaaccac gtcagcttta tctccaacct caaagttgtg ctttgagcaa cctgtggcta
     8521 gtttgcactt tgattttcat tgattattag caattgtcac tgtaaataat atccttgtag
     8581 caagcaattt ttctcctaga cttgaaataa agcgcatttc tctatataat aatactcata
     8641 tagaaaacac cttttagaaa gatacctatg cttccatatc cattttccta tttttcaagt
     8701 atttgggggt ttcgtaagcc cctgtccaaa cgtttcgagc tcaactggtt tcaacttctc
     8761 tttaccagta tcttccttat cagcttgtct atggtaccca ttgctatcca aaacagctcc
     8821 caggagacct atccgctaga aacttttatc gataatgtct atgaacctct gacaaatgag
     8881 gttgtccagg atctctctga acatgctaca attgtcgatg gcacattaac ttatactgga
     8941 acagctagtc aagccccttc tgttgtgatt ggtccaagtc aaatcaagga attacctaag
     9001 gacttgcaac tgcatttcga tacaaatgag ctagtcatca gcaaggaaag caaggaactg
     9061 acccgcatct cttaccgagc cattcagact gagagtttca aaagcaaaga cagcttgacc
     9121 caagcaattt ctaaagactg gtaccaacaa aatcgtgtct atatcagcct cttcctagtt
     9181 ctcggtgcga gcttcctctt tggtttgaat ttctttatcg tctctctagg agctagtctt
     9241 ctcctttata tcactaagaa atcacgcctc ttttcattta gaacctttaa agagtgctat
     9301 cactttatct tgaactgttt aggattgccg actctgatta cacttatttt gggattattt
     9361 ggccaaaata tgacaaccct gattactgta caaaatattc tttttgttct gtatctggtc
     9421 actatctttt ataaaacaca tttccgtgat ccaaattacc ataaatagga gatttttatg
     9481 cccgttacga ttaaagacgt ggccaaggct gctggtgttt cgccttcaac cgtaacccgt
     9541 gttattcaaa ataaatcaac cattagcgac gaaacaaaaa aacgcgttcg caaagctatg
     9601 aaggaactca actaccaccc aaacctcaac gctcgtagct tggtaagcag ctatactcag
     9661 gttatcggat tagttcttcc tgatgactca gacgccttct accagaatcc tttctttcca
     9721 tcggttctac gtggcatctc tcaagtcgca tctgaaaacc actatgccat tcagatagca
     9781 acagggaaag atgagaagga gcgtctcaac gctatttcac aaatggtcta cggcaagcgt
     9841 gtagatgggc taatctttct ctatgcccaa gaagaagacc ctctcgtaaa actcgtcgca
     9901 gaagaacagt tccccttcct tatcttaggt aaatctctat ctcctttcat cccacttgtc
     9961 gacaacgaca atgttcaagc tggttttgat gcgactgaat atttcatcaa aaaaggctgc
    10021 aaacgcattg cctttatcgg aggaagtaaa aagctcttcg tgaccaaaga ccgtttaaca
    10081 ggctatgaac aggcgcttaa acattacaaa cttaccactg acaacaatcg catctacttt
    10141 gccgacgagt ttctggaaga aaagggctat aaatttagca agcgattatt caagcacgat
    10201 ccacaaattg atgctatcat cacaaccgat agcctcctag ctgaaggtgt ttgtaactat
    10261 attgccaaac accagctgga tgtccctgtt ctcagctttg actcggttaa tcccaagctc
    10321 aacttggcag cctatgtcga tatcaatagt ttagagcttg gtcgtgtttc ccttgaaact
    10381 attctccaga ttattaatga taataaaaac aataaacaaa tttgttaccg tcaattgatc
    10441 gcccacaaaa ttatcgaaaa ataagagact gggcaaaaag tcgttaaaag caaaaacgca
    10501 tagtatcagg tattgaaaaa acttgatact atgcgtttta atgtgggaag atttacttcc
    10561 ttttctactg aaattgagtc ttttcccaag atctttttat actcaatgaa aatcaaagtg
    10621 caaactagga agctagccgc aggttgctca aaacatggtt ttgaggttgc agatagaact
    10681 gacgaagtca gctcaaaaca tggttttgag gttgtagatg aaactgacga agtcagctca
    10741 aaacatggtt ttgaggttgc agatagaatt gacgaagtca gtaacatata tacggcaagg
    10801 cgacgctgac gtggtttgaa gagattttcg aagagtatta atcactaatt atctatctca
    10861 acaaatcttc ctagaatatg aacattttcc gagacagaga caaaggagct tggatccact
    10921 tgtgtcataa tctgtttaaa ttcattaaac tctgcacgtg taatgacagt gattaaaact
    10981 gcctttctct cgtgattata ggttccttct gcatcgtgga tcatggttgc tccgcggtgc
    11041 aattttttat ggattttttc aattaccttc tctggatgat ttgtcacaat catggcctgc
    11101 atacgctttt gcttagtaaa gactgcgtct gtcacacggc tagagacaaa gatggtaatc
    11161 atagaataaa gagcgtattt ccaaccaaag gtcaaacctg ctatcagcat gatagttcca
    11221 tttaccaaga aagaaatact accgacattc ttacccgttt tcttacgaat agtcaggctg
    11281 acgatgtccg tcccaccact ggagatattg tttcgaagag caaaaccaat ccccaaaccc
    11341 ataacaacac ccccaaaaag ggcattgata atgggatcct ctgtcaaggt tgccacaggg
    11401 acaaactgga taaagaagga actcatagat accgtgataa aggtaaagac ggtgaactta
    11461 tggccaatct gataccaagc taagaccatc aaagggaagt taatggcgta aaagcttagc
    11521 gaaatcggaa tatgaaaacc aaaccagtga ttactcaagg cagagataat ctgtgccaga
    11581 cctgttgcac cactcgaata cacatgccct ggttggaaaa agaaattaac tgctactgct
    11641 gataaaaaac catagaccag agaggccgaa atcttctcat catacttttc tcgagagata
    11701 ctttgtaaga cacgtaaaat ttttatctga taagcaaagc ggcgcagata atagcgccac
    11761 cgcttaattc gttttgtttg tttcatcttc ttctacttgt aagctgagtt cctctagttg
    11821 tttgagagcg actgttgatg gagcttgtgt cattgggtca gttgccttgt tgttcttagg
    11881 aaaggcaatg acttcacgga tattttcttc tccagcaagc aacatgacaa aacggtcaag
    11941 cccgatagcc aaaccaccgt gtggtgggaa accatagtcc atggcttcaa gaaggaaacc
    12001 aaactggtca ttggcttctt cagttgagaa accaagagcc ttgaacatgc gttcttgaag
    12061 gtctttttgg ttgatacgaa ggctaccacc accaagctca taaccgttca agacgatatc
    12121 gtaagcaatg gcacgaacct tagccaaatc accttctaat tcgtgagcag tctcttcctg
    12181 tggaagtgtg aaaggatggt gggcgctcat gtagcggcct tcttcttcag accattcaaa
    12241 catcggccag tcaaccaccc aaaggaagtt gaacttatca ttatcaatca agccaagctc
    12301 tttagcaata cgtccacgaa gggcacccag tgttgcatta gccacttcaa gcgtatccgc
    12361 cacaaagaga accaagtcct tatcttcaag aacaagcgct gttgtcaatt cttcttggat
    12421 accagtcaag aacttggcaa ctggtccgtt taattctcca tcaaccacct tgacccaagc
    12481 aagacctttg gcaccatact gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga
    12541 atagttgtcc gcagctcctt tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac
    12601 tttaaagtct acacctttga ccacttctgt caagtcctga agcaacatgt caaaacgagt
    12661 atctggcttg tcagaaccgt aaagagccat agcatcatcg tatttcatac gagggaacgg
    12721 tagcgttact tcgatgcctt ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat
    12781 atcttggatt tctttctcag taaggaagga cgtttccaag tcgacctgag taaattcagg
    12841 ctggcggtct ccacgcaagt cctcgtcacg gaaacattta acgatttggt agtaacggtc
    12901 aaaaccagca ttcatcaaga gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa
    12961 atgcccctta ttaacacgag acggcactaa ataatcacgc gccccttcag gcgttgactt
    13021 agaaaggaat ggtgtctcca cgtcgataaa ctccaactca tccaaatagt tgcggataga
    13081 gtgggtcacc ttggcacgaa gtttaagatt ttccaacatt tctggacgac gaaggtcaag
    13141 gtaacggtaa cgcaaacgtg tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg
    13201 tgttgtctta gctgtgttaa gcacaataag agctgtcacg tttaactcaa ccgcaccagt
    13261 tggcaactta tcattggctt gctcacgcgc agcgacctga ccagtcacct caataacaaa
    13321 ttcgctacga aggctttcag ctgttgccat aacctctgca gatacttttt cagggttgat
    13381 aaccaactgc atgattcctt cacggtcacg aagatcgata aagatcaaac caccaaggtc
    13441 acgacgacgg ccaacccatc ctttcaaggc tatttcttgt ccgatgtgtt cctcacgaac
    13501 acgaccagca tacatactac gtttcattat ttctctcctc ttttattctg ttactatttt
    13561 accataaaag cgcaggctct tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt
    13621 cagatgaaag ccctaaaaat tagcgctaat actcttcgaa aatctcttca aaccacgtca
    13681 gcgtcgcctt accgtaggta tggttactga cttcgtcagt ttcatctaca acctcaaaac
    13741 catgttttga gctgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga
    13801 cttcgtcagt ttcatccaca acctcaaaac agtgttttga gcaacctgcg gctagcttcc
    13861 tagtttgctc tttgattttc attgagtata atacaaaaat ccgatgaact tcaccggact
    13921 cttttatttt gaatttttgc ctgctttacg cttttcagcg atttcggctg cctttcgagg
    13981 caagacaatt tccgttatgt aagccgtccc aaaacgcagt acacctgcaa taggagcaaa
    14041 gacaactgct agatagttat agaagaaatc gcctttgaag gcataagcta gcgctccaat
    14101 gatgaaaaat agaatgactg cctgaatcac tgctactaaa attactcgtt tcatgtgacc
    14161 tcctgactct attatagcat gagaatcatc aaaaagccga ctaaattatt caaagcgtga
    14221 agagaaatac tgtagaccag accttttctg ctaatgtaag ccaaacccaa actaaaacca
    14281 aggctaaaat agacaaaaaa ttgttgcaca tcacctggaa aatgaatcaa ggcaaataga
    14341 agactagata ccagaagaaa aatcagggtt cgtttactat tgtcctgctt aggaaagaga
    14401 tagcgtgcta acatccctct aaaaacaatc tcttccgtca aaggagcaaa aataaccaca
    14461 gcaaagaatg agaaaagtgg ttgagacaag gtcaagtctg tcgctatttg ttgatttact
    14521 gaaggatcat ctggcaagaa gaattgaacg accagagata agaaccaaac caaggcagga
    14581 agccaaataa atcggttaaa gccgctcttc tcaatataaa caggagcctt ctgataccat
    14641 ttgtaaatgc cgtacacata tactccagcc aaggccacat agagtagagt aacagcatag
    14701 ggtgaagcgc ctaaagcaag cgacgcagtc gcgagcccct gaataaagcc atagataaat
    14761 aaaaaggata gaagggctag aagaatccag ccaaggtttt taagtaattt catagataac
    14821 tcctttattt gaaataacgt tttaccatag gtaactgcat cacattgata taaacatgga
    14881 tggctcctac aagcaagaaa gctagtaact gaatctctcc tgtcaagaaa gaaatgataa
    14941 taagaaaaat atataaggct ggtaagacat attggtgtaa ttggaataaa attcgaaaac
    15001 tctgttccaa attagcctga cgctcccctt catcataaga atttatatag ttcaagacat
    15061 cctttggtgt agcgaaaaat tccaaatcaa actgacgaac aatcgcaatg gttttaaaaa
    15121 gagatttttg agcgactaag aataccacaa agagtaagaa agaaaggaaa aatgtttgag
    15181 ggtttgtatg caatataatc acctcactta atgaaataaa aatagccaat ggaatcgcta
    15241 cacctgtaat attaaaagca atggttccaa actcaagatt ccgatacatt tgcacataat
    15301 aggtttcatt cagatcgtca tccatttcct cttgatacaa ggaatgaaat tttctgcttt
    15361 tctttaagaa attgaaagtc aaaaacatac taatgaaacc tatcagtaaa caaatagctg
    15421 atatccatgg catcaaggct tttacatcta aaataatttc gtgggattcg acacgtgcct
    15481 taaacatccc tacaaacatg cccaagaacc ccccaagaca atagacatca aaaataacaa
    15541 tctacgtttc tttttcatat tcattctcct ttttcacttg ctagattttt ggatttcttt
    15601 tcaatccatt cagttactgg gatgagagca aagtagaccc aaacaaattg gtcgctttga
    15661 tagggattaa accagcttag gtccatccca atcagtagaa atacgctgac taataaagct
    15721 atgaccacta cataataaat cactttatac ttgttcatca ctcgtcctcc tccaaacgaa
    15781 ataccgattc gactgtttcg ttgaaaattt gagatatttt cagggcaatg ataatggatg
    15841 gggtgtactc atcccgttct agtaggctaa tggtctgtct ggaaacccct gccagtttgg
    15901 ctaggtcggt ttgattgaga ccatcgcgag ctcgaagctc ttttagacga ttttttagtt
    15961 gcatgttaca cacctactct ccgtcaaatt caacggtttg gatatcttca atacgttgca
    16021 acttgaattt ttctttcccc tttttatcaa cactacgtag tttgacccat tcctcgtcaa
    16081 catccacaac ttcccagtta tctggcccaa tatacactcc cgttataatt ggttcctttc
    16141 caatcatttc ttgtaataat ctcgacattt ctgcgtttcc tttcttcttt tctcttaact
    16201 tgcttacatt gtaacacatc tttttctttt tgacaagtat agttgtcaaa aagtttatga
    16261 tttttgtcat tttgcaaagg aaaaaggtca ggagtaagtt cctgaccact ttatctatca
    16321 ttaatactct tctaaaatct cttcaaacca cgtcagcttc accttgccgt aggtatggtt
    16381 actgacttcg tcagtttcat ctacaacctc aaaacagtgt tttgagcaac ctgcggctag
    16441 cttcctagtt tgctctttga ttttcattga gtataaaatc ctaatttttc aaagatttct
    16501 gagaagtttt ggctgattgt ctcaagtgac acttgcactt cttctcgggt ttggttgttc
    16561 ttgactgtca cttgtctgct ttcgacttcg ctctctccta gggtgatgag ggtcttagcc
    16621 gcaaagacat cggctgactt gaactgagct ttgagcttac ggttgaggta atcacgctct
    16681 gctttgaaac cttgttggcg aagagcttgg actagttcta gggctttgac atttgctcca
    16741 tcacccaaga ctgcgatata gacatctagg gcattttcga tagggagggc cacgccttgt
    16801 ttttcaagga tgagaagcag gcgctctaca ccaagcccaa aaccaaatcc agcagtttca
    16861 gggcctccaa agtaagcaac caaaccatcg tagcgaccac ccgcacagac tgtcaagtca
    16921 ttgccctcaa tctctgtgat aaactcgaaa atggtgtggt tgtagtagtc cagaccacgc
    16981 accatattgg tatcgatgat gtaatctact ccaagatttt ccaacatctg acgcacagca
    17041 tcaaaatgag cttggctttc ttcatcaaga aagtccaaga tagacggcgc attctctact
    17101 gccaccttgt cttctttttc cttagagtcc aagacacgaa gaggattttc ctccaagcga
    17161 cgttggctat ccttagacaa ggtctccttg agcggtgtca aatagtcaat caaggcttgg
    17221 cggtaggctg cacggctctc aggatttcca agagtgttga ggtgcaattt gacaccttga
    17281 ataccgattt ccttcaaaaa atgggctgcc ataacgattg tttccacatc ggtagctgga
    17341 ttgctagagc caaaacactc aacaccaatc tggtggaatt ggcgcaagcg ccctgcctgt
    17401 ggacgctcat aacggaacat aggtcccata tagtagaact tgcttggctt ttgcacttct
    17461 ggggcgaaga gtttattttc cacataggca cggacaacgg gcgcagttcc ttctggacgg
    17521 agggtaatat ggcggtcacc cttgtcataa aaatcgtaca tttccttggt tacgatatcc
    17581 gttgtatctc cgacagagcg actgataacc tcgtaatgct caaaaatagg cgtgcgcact
    17641 tctgcatagt tatagcgctt gaaaatctca cgggcaaagc cctcaacgta ctgccacttg
    17701 gcagattcag caggtaaaat atcctgcgtt ccttttggtt tttgtaattt catagggaat
    17761 cctctttaaa cttaatagtc ttattttacc ataaataaag ggattaaaac agtaagaaaa
    17821 aaattaggat ttagatatca tttttgagat taagaattgt caaaaaaata gctagcaagg
    17881 aaaaaccaac aaatagcatc caagtcaact gtatattcca tacggctact agtgaaaaac
    17941 aagctgttcc cacaggtatg gataaggtaa acaatagacc taaaaaatta ctagtacgag
    18001 ctagaacctc tggagctaga tttttcatga gcatggcact aatctttggt tgaactttac
    18061 cagacacata cagagtaaag aagagaaata gcaaaccaag cacgacttga ttgaataaat
    18121 tagccaaacc aactagacta agtcctacgg tctcccacat catcaatcta ggcaaggact
    18181 gcttcccaaa ataatcattg cccgtaaggc tactaatgat gattgctaac aaaacacaga
    18241 cttgattgat aaatagtgcc tctgtataag aaaaattcaa gagagaatgg ctcaaaaaga
    18301 agatattata aattccaccc aaagcgccac ccaaggaatt aataagcaag acagcaaaga
    18361 gcataaaacc aaagtttttc tgtccacttt taagaaaaac gagacgtaaa tttcggtaaa
    18421 ttgttaggaa ctggtctttg atagaaagct tctcattttt tagtttttca ccatcagcag
    18481 atgaaattga caggctcaat ttgctttttc ctaaaaaaag aataaaggct gatacgagga
    18541 aaaagcaagc attaattccc gcaacgaggg aaaaattgtt gaccgataga gctaagagcc
    18601 agactccgaa agcttgacca ccaatagctg aaatataggt gatgaactgt gaaaaagaat
    18661 aagcctccat cagatcatct tcagctactt tttccttaat aagaggcata cgcaggccac
    18721 ctgcaaaatc actgatgata tcactaatga cattgatcaa acacaggcta gaaaaggcaa
    18781 agagactagc ttgctgaaca actagggctg ctagaaaaaa tagaaccgcc tgaaacaaac
    18841 cgctatagac catccatttg accttgtccc tcgtgtaatc tgcccgaatc cctacaaaaa
    18901 ctgtaaagag ggtcggaaga atcatgacaa tattcgccat agcaacagca aaagatgctt
    18961 gtgacaaggt cgatgcatag acgataaaga ccaggttgaa aatcgaagca ccaaaagcat
    19021 tgaagaagcg tgataaagtc aaaattctat aagctggatt tctaaacaat agtttcatag
    19081 ataatctccc tccttgttct aagttctaca tcaagaatag ctttagtata gcacttatga
    19141 atttgcgaaa cgattctttt gaatatatgc aacaaacaaa atgagcaaaa aaacgataga
    19201 acttttgtaa tgacctgaga gagggcaaca gttctatcgc ttcaaatttt ttcttggttt
    19261 gcagatattc aagaatcggg agtttttcta tagtattcgg cagatttatt acagccaagc
    19321 atctcaaaaa tacggacagc atcctccatc tttttctggc cttccttgac tctaccttgc
    19381 ttgctatcaa ggagaccttc tgcccacaga taaacaattc ggaaataggt ctcattttcc
    19441 ttgtagaaat gctcttcgat aacacgttta aaataatagg cattggtaaa ttcttcacac
    19501 tcaatactag ctaaaaagcc attcaatagt atagtatgaa aaaggtttcg attgccagac
    19561 atttccatta gaaaatcaga tttacgtacc atttctcgta catatctagt aaaaagagaa
    19621 acagataaaa atggagaact gactgaaaat aaattgagtt catagattcc ccagatctcg
    19681 gtagaaaaca aataatcatg aaggactttt ccttcctctg ctgttaagtc taccctttca
    19741 tctatgctct tcatataaga cttgataata atggcattta gaatatgttt ctgtttgttg
    19801 tgagaatggg catgcttttg atactccctg cgatataagt cctcaagagg tgctatattc
    19861 tttggttcca agacatctgt aatttctttt ctcaactcag aatctgtatc atactggaaa
    19921 cctctcgcca gaaagaggat ctcctccaca ctggcagata tattttccag agcaaataga
    19981 aacttttcca ccgaaagctc actctgacct gtttcaaaac gggacaacat agacggcgaa
    20041 aattgtcctc cggttgcttg tctcagtgag atatttcttg actctcgtaa ttgtctaaag
    20101 acttttccaa tctgctccat agacttcccc ttgattccgt attttcttca ttttatcata
    20161 tttttcagaa aattcatcaa aaacttgcca aattgtcaga attatgagaa aatagaggat
    20221 atttatcacg tggagggact gctatgagag acgatatcaa aatcaatgac cgtgctttgg
    20281 ccttgcaaga ccaaatcatc gaaaaactag agaaagtttt tgatacagat gtggaattgg
    20341 atgtttacaa tctaggtctg atttatgaaa tcaatctgga tgaaacgggg ctctgcaaga
    20401 ttgtcatgac cttcaccgat actgcctgtg attgcgccga aagcctgcct attgaaatcg
    20461 tggcaggtct gaaacaaatc gagggtatca aagatatcaa ggttgaagtt acctggtcgc
    20521 ctgcttggaa aatcacacga atcagtcgct atggccgtat tgcccttgga ctaccacctc
    20581 gttaagcagg ccaatcattt ttaaagatga aaaaagcaag ccaaaaatgg cttgcttttt
    20641 gatactcaat gaaaatcaaa gggcaaacta gaaaactagc cgcaggttgc tcaaaacact
    20701 gttttgaagt tatggataga actgacgaag tcagctcaaa atactgtttt gaggttgtgg
    20761 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
    20821 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacact
    20881 gttttgaggt tgtggataga actgacgaag tcagtaacca tacctacggc aaggcgacgt
    20941 tgacgtgatt tgaagagatt ttcaagtatg agtttatttt ttacctgact tgtccatatt
    21001 ccagaagtct gtcacggctc cgcgtgaagc agatgatacg atatgggcat atttaccgag
    21061 gacaccacgg ctgtaaagtg gtggcaaggt tgtttctgcc ttgcgttttt caagttcttc
    21121 ttcggatacg gccatggaaa tttctttggt atcttggtca accgtaacga tatcgccggt
    21181 acggagatag gcaattggtc caccatcctg agcttcagga gcgatatgtc caacaaccag
    21241 accataagta ccaccagaga aacgtccgtc cgtcaagagg gccaccttat ctccctgacc
    21301 tttaccaaca atcattgaag aaagtgacag catctcaggc ataccaggac cacctttagg
    21361 tccaacaaaa cgaacaacga ctacatcgcc atcaacgatt tcatctgtca gaacggcctg
    21421 aatcgcatct tcttctgagt caaagacctt agctggccca acgtgacgac gcactttaac
    21481 acctgatacc ttggcaactg caccgtcagg agcaaggttc ccgttcaaga tgataagcgg
    21541 accatccgca cgttttggat tttcaagtgg catgataact ttttggcctg gagtcaagtc
    21601 tgcaaagtca gccaagtttt cagctacagt cttaccagta catgtgatgc gatctccgtg
    21661 aaggaaacca tttgccaaca agtacttcat aaccgcaggg acaccaccga cttcgtagag
    21721 gtcttggaag acatactgac cagatggttt caagtcggcc aagtgaggca cacgttcttg
    21781 aatcgtattg aagtcctcaa gtgacaagtc aacatttgcg gcatgggcaa tggcgagcaa
    21841 gtgaagagtg gcgtttgtag aaccaccgag agccatcgtt acagtaatag catcttcaaa
    21901 ggcttcacga gtcaagatat ctgatggttt gagaccaagt tccaacatct taacaacagc
    21961 acgtcctgct gcttcgatat cttctttctt atcagctgat tcagctgggt gagaggatga
    22021 ccctggcaaa ctcatcccta gaacttcgat agcagttgcc atggtattag cagtatacat
    22081 accaccacaa ccaccagggc cagggcaggc attacattca agacgtttca cgtcctcagc
    22141 tgtcatgtca ccgtggttcc attttccgat accttcaaag acagaaacca agtcgatatc
    22201 tttaccatca agatttcccg gtgcaatagt tccaccatag gcgaaaatag ctgggatatc
    22261 catattagca atagcaatca tagatccagg catgttcttg tcacagccac cgatagcgac
    22321 gaaggcatcc acgttgtgac cactcatagc cgcctcgatg gagtccgcga tgatgtcacg
    22381 agatgttaga gagaaacgca taccaggcgt tcccatagcg atcccgtccg ctacggtaat
    22441 ggttccaaac tgtacaggcc aagcgcctgc agatttgaca ccttctttag ccagtttccc
    22501 gaaatcatgc aagtgaatgt tacatggtgt attttccgcc caagtcgaaa tcactcccac
    22561 aatcgatgtt tcaaagtcct tatctgtcat accagtcgca cgaagcatag cacggttagg
    22621 tgatttaacc atgctgtcat agatgctact gcggtgacgt ttatctaatt cagtcatctt
    22681 atccctccca tttcagtttt tactattata gcacaattta aaggcaatga acagaataaa
    22741 attcttgaat tttcagaaaa ttctatacgc atgtgaaata tttaaaatta aaaacaacaa
    22801 agcggattag tgcactttct gatgaccaga atatgctttt taatccgctt tctttaaata
    22861 acgtactgta atttttacag aaattctttc agataagtgt atttaacatc tatcttgcat
    22921 tataaatttc tagaaccttc tcttttatat tcgattcact caaaccatac tcattaagaa
    22981 gataatccat tttccctact tgaccaaatc tttcttgaac acccatccga tgaatttttg
    23041 tcattccatc atcagagaat aattcacata aagaactgcc aattccacct atctgattgt
    23101 ggttttctac agtaaatata gtttttccac ttaacattgt ttttatctgt tctggtatcg
    23161 gtttgattct aaataaatct atcacactta ctgaataacc taatttagac agttcatctg
    23221 caactcgaat acttggagca accattatac cagaagcaac gattacaaga tcttcaccat
    23281 gccttaactc aatgtagcct ttagaaaaat cttctccacc ttgatacaca ggaactggag
    23341 cttttctaat tgttcgaata tattttagtc cttttaagtc caatgtctgg ttcaatattt
    23401 cacgaaattg gatatcatca gttgcttcaa aaatgatcga tttaggaatt aaacgtaaca
    23461 atccaatttc ttcaaatggc atatgtgtcc caccattcat ttctgcagtt actcctgcat
    23521 ctgatccaat cacagtggca tccaattgtg cgtatccaag agaaataaat aattgatcaa
    23581 atactcttcg tgaagcaaaa ggaccaaatg tatgaagata aggtctaaac ccctgaatag
    23641 acaagcctgc tgcaaggccg accatttctg cttccataat cccaacattc acataacggt
    23701 ctccaaagtc cttttcaaga ttatgagtag ccatcgaact tgacaaatcg gcttctaaga
    23761 ctactatatc agaataactt tgattagcct ctaaaaggaa gtctctatat acatgccgta
    23821 attctttcgt acttctcatc attctgtttc ctccaattcc tgacttaatc tttctacaac
    23881 tgaagttaac atttgtttct cctctacagt aaggcgaaga tgatgattgg atttcatttc
    23941 ttccagctct tgaacccctt gacctttaat agtatctaat acaatgcact taggtgatga
    24001 attatttgac tgttttaatt ggacaatccc ttcataaatt tctctaatat ctgaaccctt
    24061 gaccctaatg gattcaaatc caaatgctga aaatttttct acgaaatcac ctggattaca
    24121 aatatccttt gtaaaaccat ctaattgttt tttgttatca tcaacaaata caattaagtt
    24181 ggataactgt tgatgagaag caaactgtat agcctcccaa cattgtccct catttaactc
    24241 accatctcca acaatagcat aagtataaaa gggactcttt cttattctct gaccatatgc
    24301 aagtccagtt gcaacactaa ttccttgtcc taaagagccc gttgtcatat ctatgcctgg
    24361 cgttagattt ctatcaggat gagacggtaa tttggttcca tttgtattta aagaatatag
    24421 gaattctttg tcaaagaaac cattcaaata gagtgtactg tatagagctg gtcctccatg
    24481 accttttgat aatatgaaat aatctctatc tcgtgctgca aatatttctg gagtcattgg
    24541 cattatttca ccataaagca ctgctaaaac ttctacgata gacagacttc ctccgtaatg
    24601 tccgaatcca agatgattca atgttctaag agtatttaat cggatgttag tcgcaaattt
    24661 tcttaaccca tcttctctat ttttacttaa aatcatccct tattcctcct ttgcagatgg
    24721 ctttttaata aaggatactc caaacataac tgctagaata agaacaagac caatcacaat
    24781 gcctgcttgt gagccaaatt gatttaacat tcctaaaata attcctgata gaccaaaatc
    24841 tgcatctgag aaagttgatc cttggaaacc aagtcctccc aaaactggca ttaaaaagac
    24901 tggaagaaaa ctgattaaaa taccttgtaa aaatgctcca atagtggctc cacgaacacc
    24961 accagatgca ttcccaatga cacctgcagt cgctccacag aagaaatgag gcacaacacc
    25021 tggtaagata acaaccgttc ctgaagcaat cataattgcc atacttacta aaccaccaac
    25081 aaaactagag ataaatccaa ttagaactgc attgggtgca taagtataaa caatcggaca
    25141 atccaaagca ggttttgaat taggtacaag acgctctgaa atacctttaa aggctggaac
    25201 aatttcaccc aaaataaggc gaacacctgc taaaataaca aatacccctg ctgcaaattg
    25261 acctgctaat tgtaaagcat aaactagacc acttgtacca ctactgattt ctttttctat
    25321 atattctgac cctgcaaaga tagctacaat aatgtaaata actgccatgg ataaagtgat
    25381 actaacagta ctatcacgta aaaaagctaa actctttgga aatttaatgt cctctgttga
    25441 ttttgatttg tcaccgataa ggctaccagt aaaaccactc aaccaatatc ccaaagaact
    25501 gaaatgacct aaagctacct tgtcatttcc agttaattga accatatatt tttgcacaaa
    25561 tgctggggaa atactcataa taataccgag tgctaatcct cctagtaaga tgagaggcaa
    25621 gctagtaaag ccagcaactg ataaaatgac cgcaatcata catgccatat atagagtgtg
    25681 gtgccctgtt aaaaaaatat atttaaatcg agtaaaacga gcgattaaga tattgaacac
    25741 catgcctgca aacataatca ttgcagtagc tgagccatat gttgttaaag ctacagctac
    25801 aattgcttca ttattcggca caacgccaga taaatgaaaa gcatgctcaa acatggtacc
    25861 aaatggattt aaagaatttt gtacaattcc tgcaccacca gatacaacta agaaaccaac
    25921 aaaggtctta attccacctt taataatatc aggtaatttc ttcttctgaa gaactaatcc
    25981 taagattgca attaaagcta ctaaaatagc tggtgtacta acaatatcca atatgaactt
    26041 catcatgacg ctagcctcct atataagtcc tttttcttca caaagtttag taattaattc
    26101 tcgtagttca tccatatcaa taatactatt taagatacga acatctccaa gatgactagc
    26161 tgaatcagct agatcacgac caacaatcca aatatcagct gcatttggat ctgctccacc
    26221 taaatcataa tgttcaactt ctacatccga aacattcaaa tcactcaata cagattcaat
    26281 attcatctgt accataaaac ttgaacctaa tcctgaacca caagctgtac caatttttaa
    26341 cattatctaa tcctcctgtt taattatcat tttaatgtca tcatagtttt ttgatgatat
    26401 taaagtttga acatgatttt tatctcttaa aattgttgtt aaatgtgaca aagcctttaa
    26461 atgactctca ttatcaatgg ctgcaataca aatcaacaat cttacctctt gttctggatt
    26521 atccaataaa taaatcggtt cttccaaaac taacattgac attcctattt cattcacacc
    26581 ttcatctggc cgagcgtgag gaattgctac tcccttccct aaattaataa aaggcccaaa
    26641 ctcttctact ttttgaatca ttgcctcagg gtagttctca gttatcttat cttgatccaa
    26701 aagcggttta gctgctaaac gaatcgcctc cttccatcct aatttttgcg aactaacctg
    26761 ataggtttct ttggtaataa gttgttctag cactggtaca atttcctttc tatcattttt
    26821 ttgataaaga taattcttta acgccaatct taattccaat tcttgtgtaa taattccata
    26881 tcttttgaca atattcagga tttgttcaat ctcaaaatcc ccatactcta aattcggaaa
    26941 atcttttaac actagttcta ctagttgtat tgcttgctct tcagtcatca taaccgaaac
    27001 tagataattt ggcttttctg tctccacctt tatggtagaa aaaaccatat catagtcact
    27061 actagctttc acctgtaaat catcaatctt tgaggttcct ataaactcaa tttgaggaaa
    27121 taatgctaat agattctctt ttaacatcaa tgaagaacta acaccattag gacaaatgat
    27181 tgctgcttta taccattttt gaggcaaagt atctgctttc tttaaataac ctccgaaatg
    27241 gataacaaaa tatgctgttt cactatcagg tatgggattg tcaatagcgt ccatcaaggg
    27301 catcaaagaa tctttgacta attcaaataa atcaggataa tgttctttaa catgcaatac
    27361 atattcattt gaactaggta ggccgaactt taatctatag taagccggta taaggtggcg
    27421 acgaagattt tctctcaatc cttccctttg tttaaaatgt aacaaagaaa tatcttccat
    27481 tctacttata atagcctctg ttaattgatt aaagtaaacc ggagcaacat ctacttcacc
    27541 ttcaaagcaa cttgataata aaactgtgat atagcgataa tcatcctcag aaaacaccgt
    27601 atctataatt cccaaatcaa ccactgtatc caataaaata gtcgttatat cttgaataac
    27661 aggagatact aatgtctctg aaagacatac tctttcaaca tccctttgat acctacacag
    27721 aatgaatact aaaccgaaaa ggtaaacttt taattgatta acaataggta ctagctgtag
    27781 cttctcataa taatctttaa ctacctgatc aatcaaatca taagttaatg aataccccca
    27841 actggataaa acataatcca aaccccaaat ccctatggag gattccagca actcactaac
    27901 catttgaaaa gctaagcggt gcttattcca ctctgaaccg tgtaaagtat aaccttttgc
    27961 tctactgtac cctagctcca aatcattatc taacataatc tttcttaatg attgaatatc
    28021 agataaggtt gtattcttac ttactttcaa aaagtcttgg taatgattat tcgatataaa
    28081 atctaatcgg caaaaagtgt aaagatagat taaagctaag cgagtcgact ttggtaaaac
    28141 caattcatcc gacttaataa tatctgtcaa agactgcttc gtacgatttg ataaactata
    28201 gcgaccttgc tttttatcca gcactatccc tttattagct aaataaggca ctaaataatc
    28261 tattccttct ttgacttcct ttataggtaa gctcacctta acagataact catataatga
    28321 tagctcacaa tgatccatca aagtcatcaa aataactagt gctctataat caaacatttc
    28381 tcttcctttc taactacaat tatagtgtaa aagttataag aataaaagag tatttgagca
    28441 caatataatc tcacctttta aaagaaatta ttgggctgaa agtttttagt gattattttt
    28501 caactttcta ttgactttta aaaacaaagt gatatcattt agatatcaaa taatgggagg
    28561 tcattcttat gactgaaaaa ctaatcaatt caaaaccaaa tggtgtattc gcattgattc
    28621 tcattgaatt gacgctcgta cttggtatct ttatatttat aatgggtgtt ggttcggaaa
    28681 acatttttgg aattattatc ggacctttac taatcgtaat tgcaggtcta gctcatgctg
    28741 gtttaaaagt tgtcaaacct caggaagctc tggttctgac actctttggt aattatacag
    28801 gtaccatcaa agaacctggt ttttactttg tcaatccctt cagcgtagca gtcaaccctg
    28861 caaaccacac tcgacttgga caaagtggtg atgttagcac aaaatctcct tttttaggtg
    28921 ctaaatcatc aaatgacaat gatgtaaatc ttgaaattgg caagaaacac atttccctca
    28981 aagtcatgac cttgagcaat tctcgtcaaa aaatcaatga ttgcttagga aaccctgtag
    29041 aaatcggtat cgcggtaact tggagagttg tcgataccgc taaggcagtc ttcaacgttg
    29101 ataactacaa agaatatctt tcattgcaat gtgatagcgc cctccgtaat attgtccgca
    29161 tctatcctta cgatgtgtct cctaatgtgg atactacggg tgacgggcaa gcagatgaag
    29221 gtagtctccg tggctctagc gaaattgttg ctaaccgtat tcgtgaagaa atccaaaatc
    29281 gtgttgagga tgctggcttg gaaatccttg aagcacgtat cacttaccta gcttatgctc
    29341 cagaaattgc tgccgttatg cttcaacgcc aacaagcatc tgccattatt gatgcacgga
    29401 agatgattgt agatggtgct gtaggaatgg ttgaaatggc actagaacgg ctcaatgaag
    29461 gggaattggt agaacttgac gaagaacgaa aagctgccat ggtttcaaat ctccttgtcg
    29521 ttctttgtgg caatcatgat gcacaaccaa ttgtcaacac aggaagtctt tactaaagat
    29581 ggctgaagct aaaaaaaagc agatccccct tcgactctca acaaagttat acgctgcact
    29641 cgcatcatgg gcagaagacg actttcgttc agtcaatggg caaatcgaat atctcctcac
    29701 agagtgtgtc aagcaacgga agaaagacgg aaaatacgta tcagaaacca ttgacgaacc
    29761 atttgagatt gatatttaac accttctcct gtctgccgtg tcggatagga gaattttttc
    29821 atcttttttt gtcaagtaac tttactttac aaaaaaaatg tgttatccta gtatggttga
    29881 tgaaaatcag tagattgaat cgaatataaa tacctaaagg agaaatcaaa atggcagtac
    29941 ctgcacgtcg cacttcaaaa gcgaagaaaa acaaacgtcg tacacactac aaagtaacag
    30001 ctccatctgt aaactttgac gaaactactg gagattactc acgttctcac cgtgtatcac
    30061 ttaaaggata ctacaaagga cgtaaaatcg ctaaagctgc atcagctgaa taatagaagg
    30121 gagataccat gcgcgtaaat attacacttg aacacaaaga atctggtgaa cgcttgtacc
    30181 ttacttctaa aaacaaacgt aacactccag accgccttca attgaagaaa tactcaccaa
    30241 aacttcgcaa acacgttgtg tttacagaag tgaagtaagc attcaatacg aatcaataca
    30301 gaagaaaaac gcttgatcag cgttttttct ttttgttaat ttcactatat tgcaagagaa
    30361 atcattcgaa tgactacatt ttttgaaata agaatacgga ttcggacaat taaaagcaag
    30421 aggaactcaa atgtaagttg gggttccttt tttgtatatt tttcaaataa cgcctctaca
    30481 ctatttgtag caaattcacc aactacagtt gtatcttagt taaaataagt tagaatatgt
    30541 aagtgagtac cagatatacc aagacatcgt caccatctaa ggtatattca aaatacaaaa
    30601 gttgaccaac tagatttctg aatatcctta tatatccatt cttaaaattg gtttaaatag
    30661 cgtagtcttt taaactagtt ttaagaatcc aaaaaatctt cctacatatg taagaagatt
    30721 ttttagttca gaatgattag atttagctaa tggataccta tcctacccac tcaccgttag
    30781 cattgactcg atagccatcg ggtgtagtcg tgtttactaa taaatctcct gaattatagg
    30841 tatagtacca tttaccagaa actgtaaacc aaccagtagc cattgaacct gattcattta
    30901 agtaatacca taagcctttg tctttaaccc aaccagtagc cattgaacct gattcgttta
    30961 agtaatacca taagcctttg tctttaaccc aaccagtagc cattgaacct gattcattta
    31021 agtaatacca taagcctttg tctttaaccc aaccagtagc cattgaacct gattcattta
    31081 agtaatacca taagcctttg tctttaaccc aaccagtagc cattgaacct gattcattta
    31141 agtaatacca taaacctttg tctttaaccc aaccagtagc cattgaacct gattcattta
    31201 agtaatacca taaacctttg tctttaaccc aaccagtagc cattgaacct gattcgttta
    31261 agtaatacca taaacctttg tctttaaccc aaccaactac tccctgatta gaattctttt
    31321 taggtttagc tacatcagtt tcttgtttta tattatctac tttttcaaca atgatatcat
    31381 tactagtaga taaatttgat ttaagaacat cttttatatg ttctgatgtt acatagaact
    31441 taacagtttc aggaattgca aatgtatctt ttttcacaga aaactctgta cttttattct
    31501 taatatgaat ttcctttaat gaatctaaga cgccacttag gaagaaggac ggcaatgagt
    31561 tgatattatt accaattgtt aaacttttta attttggtaa accgttcata acgtgtttac
//
LOCUS       DAYYQN010000019        29887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYYQN010000019 DAYYQN010000000
VERSION     DAYYQN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.019"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     misc_binding    37..254
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            367..1371
                     /gene="trpX"
                     /locus_tag="ACHBRQ_000817"
     CDS             367..1371
                     /gene="trpX"
                     /locus_tag="ACHBRQ_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9592521.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            1440..2306
                     /locus_tag="ACHBRQ_000818"
     CDS             1440..2306
                     /locus_tag="ACHBRQ_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592522.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKILTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            2303..3061
                     /locus_tag="ACHBRQ_000819"
     CDS             2303..3061
                     /locus_tag="ACHBRQ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592523.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            3151..4911
                     /gene="dnaG"
                     /locus_tag="ACHBRQ_000820"
     CDS             3151..4911
                     /gene="dnaG"
                     /locus_tag="ACHBRQ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9592524.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            4914..6023
                     /gene="rpoD"
                     /locus_tag="ACHBRQ_000821"
     CDS             4914..6023
                     /gene="rpoD"
                     /locus_tag="ACHBRQ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9592525.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            6037..6366
                     /locus_tag="ACHBRQ_000822"
     CDS             6037..6366
                     /locus_tag="ACHBRQ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9592526.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            6481..7524
                     /gene="cpoA"
                     /locus_tag="ACHBRQ_000823"
     CDS             6481..7524
                     /gene="cpoA"
                     /locus_tag="ACHBRQ_000823"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HFM9592527.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            7540..8865
                     /locus_tag="ACHBRQ_000824"
     CDS             7540..8865
                     /locus_tag="ACHBRQ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9592528.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            8896..9072
                     /locus_tag="ACHBRQ_000825"
     CDS             8896..9072
                     /locus_tag="ACHBRQ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9592529.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            9131..9265
                     /locus_tag="ACHBRQ_000826"
     CDS             9131..9265
                     /locus_tag="ACHBRQ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9592530.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            9329..10639
                     /gene="obgE"
                     /locus_tag="ACHBRQ_000827"
     CDS             9329..10639
                     /gene="obgE"
                     /locus_tag="ACHBRQ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9592531.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            10649..10807
                     /locus_tag="ACHBRQ_000828"
     CDS             10649..10807
                     /locus_tag="ACHBRQ_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592532.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            11894..13153
                     /locus_tag="ACHBRQ_000829"
     CDS             11894..13153
                     /locus_tag="ACHBRQ_000829"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9592533.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            13169..13720
                     /locus_tag="ACHBRQ_000830"
     CDS             13169..13720
                     /locus_tag="ACHBRQ_000830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592534.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            13713..14990
                     /gene="spxR"
                     /locus_tag="ACHBRQ_000831"
     CDS             13713..14990
                     /gene="spxR"
                     /locus_tag="ACHBRQ_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HFM9592535.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            15006..15866
                     /locus_tag="ACHBRQ_000832"
     CDS             15006..15866
                     /locus_tag="ACHBRQ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFM9592536.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            16247..17053
                     /locus_tag="ACHBRQ_000833"
                     /pseudo
     CDS             16247..17053
                     /locus_tag="ACHBRQ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            17244..19535
                     /gene="pcrA"
                     /locus_tag="ACHBRQ_000834"
     CDS             17244..19535
                     /gene="pcrA"
                     /locus_tag="ACHBRQ_000834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9592537.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            19578..20258
                     /gene="radC"
                     /locus_tag="ACHBRQ_000835"
     CDS             19578..20258
                     /gene="radC"
                     /locus_tag="ACHBRQ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM9592538.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(20255..20944)
                     /locus_tag="ACHBRQ_000836"
     CDS             complement(20255..20944)
                     /locus_tag="ACHBRQ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9592539.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(20962..21603)
                     /locus_tag="ACHBRQ_000837"
     CDS             complement(20962..21603)
                     /locus_tag="ACHBRQ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9592540.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(21686..21901)
                     /locus_tag="ACHBRQ_000838"
     CDS             complement(21686..21901)
                     /locus_tag="ACHBRQ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HFM9592541.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(21909..22256)
                     /locus_tag="ACHBRQ_000839"
     CDS             complement(21909..22256)
                     /locus_tag="ACHBRQ_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9592542.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(22261..23376)
                     /locus_tag="ACHBRQ_000840"
     CDS             complement(22261..23376)
                     /locus_tag="ACHBRQ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9592543.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(23386..24345)
                     /locus_tag="ACHBRQ_000841"
     CDS             complement(23386..24345)
                     /locus_tag="ACHBRQ_000841"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9592544.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(24458..25027)
                     /locus_tag="ACHBRQ_000842"
     CDS             complement(24458..25027)
                     /locus_tag="ACHBRQ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9592545.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            25155..25826
                     /locus_tag="ACHBRQ_000843"
     CDS             25155..25826
                     /locus_tag="ACHBRQ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9592546.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            25810..26628
                     /locus_tag="ACHBRQ_000844"
     CDS             25810..26628
                     /locus_tag="ACHBRQ_000844"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9592547.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            26625..27521
                     /locus_tag="ACHBRQ_000845"
     CDS             26625..27521
                     /locus_tag="ACHBRQ_000845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9592548.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            27565..28539
                     /gene="pta"
                     /locus_tag="ACHBRQ_000846"
     CDS             27565..28539
                     /gene="pta"
                     /locus_tag="ACHBRQ_000846"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9592549.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(28800..29887)
                     /locus_tag="ACHBRQ_000847"
                     /pseudo
     CDS             complement(28800..29887)
                     /locus_tag="ACHBRQ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8976 a         5368 c         6679 g         8864 t
ORIGIN      
        1 atagactatt gacaagtagt ttaaaaatga tataatttaa ccattcagaa aagtaatcat
       61 acaaactttt tagagagtct gtggtagctg aaaacagata agtggcgatg atgaaaattg
      121 ggctgaatgc tatttagaat ttgaaattat aaaaattcgg taagcacacc ttacagtgca
      181 tctcgttatt gcgagactga gcgataggga aattccctat aattgaggtg gtaccgcgca
      241 tcgacgtcct cacacaagtt ttttgtgtga ggattttttt gatggaggtt agtatggaaa
      301 gaaaacgatg gcgtcgcttg tttagataag tgaaatatgt taaaggaaat aaaaaggaga
      361 aacagaatga aaaataaacg tttaattgga attattgctg cattagcagt cttagtagca
      421 ggaagcttga tttattcttc aatgaataaa tcagaagctc agaataataa ggatgagaag
      481 aaaataacta agattggtgt gcttcaattt gtgagccatc catcccttga tttgatttat
      541 aaagggatcc aagatggact tgcagaagaa ggatataaag atgatcaagt taaaattgat
      601 tttatgaact cagaaggtga ccaaagtaag gttgcgacaa tgagtaaaca attggttgca
      661 aatgggaatg accttgtggt tggtatcgca acaccagcag cccaagggtt ggctagtgca
      721 acaaaagacc taccggttat catggccgct attacagacc caattggtgc taacttggtt
      781 aaagatttga aaaaaccagg tggcaacatt acaggggtat ctgaccacaa tccagctcaa
      841 caacaagttg aactcatcaa ggctctgaca ccgaatgtga aaacaatcgg agctctttac
      901 tcaagtagcg aagacaattc aaaaacacag gtcgaagaat ttaaggctta tgctgaaaaa
      961 gcaggtctga cagtggaaac atttgcagtt ccttcaacaa atgaaattgc ctcaactgtc
     1021 actgttatga ctagcaaggt agatgctatt tgggttccaa ttgataacac cattgcatca
     1081 ggatttccaa cggttgtctc tagcaatcaa agttctaaga aaccaattta tcccagtgcg
     1141 acagctatgg tagaagtagg tggtttggca tcagttgtaa ttgaccaaca tgaccttggt
     1201 gtggcaacag gtaaaatgat tgtgcaagtc ttgaaaggtg caaaaccagc cgatacccca
     1261 gtcaatgtct tttcaactgg taagtcagtc atcaataaaa aaatagcaca agaactaggt
     1321 attactattc ctgagtctgt tctcaaagaa gcaggacaag tcatcgaata aataaggtag
     1381 ggaggaggag actcttccca ttttttaaaa agatgaatat agaaaggatt aaataaaata
     1441 tgattgtttc cattatttct caaggatttg tctgggctat tctaggtctg ggaatcttta
     1501 tgacatttag gattttaaac tttccagata tgacgacaga aggttccttc cctcttgggg
     1561 gagctgttgc tgtcactttg ataaccaaag gcgtgaaccc atttttagcg acacttgttg
     1621 ctgtaggagc aggttgtttg gctggaatgg cagcaggcct tctttataca aaagggaaga
     1681 tcctaacctt gctctcaggg attttggtga tgacttcttg tcactcaatc atgctcttga
     1741 ttatgggacg tgcgaattta ggcctgcttg gaaccaagca aattcaggat gttttgcctt
     1801 ttgattcgga tttgaatcaa ctcttgacag gtctcatctt tgtgagtatt gttattgctc
     1861 tcatgctctt tttcttggac actaaactcg gacaagccta tattgctaca ggggataatc
     1921 ctgatatggc tagaagtttc gggattcata ctggacgcat ggagctcatg ggcttggtct
     1981 tatcaaatgg tgtgattgcc cttgcaggtg ccctcattgc tcagcaagaa ggttatgccg
     2041 atgtgtctcg agggatcggg gttatcgttg tggggcttgc aagtttgatt attggagaag
     2101 ttattttcaa gagtttgagc ttggcagagc gtttggttac tatcgttgta ggttctatcg
     2161 cttatcaatt tttagtgtgg gcagttatcg cacttggctt taatacaagt taccttcgtt
     2221 tatacagtgc cttgatttta gcagtctgcc tcatgattcc aacatttaag caaacaatct
     2281 tgaaaggagc caagttaagc aaatgacagc aattgtagaa ttaaaaaatg caaccaaaat
     2341 cgttaaaaat ggctttgatg aagaaaagat tattttaaat gatgtttcct tagaaatttt
     2401 tgaacgggac tttatcacga ttttgggcgg aaatggtgct ggaaaatcaa ccctctttaa
     2461 cactatagca gggaccttat cactaactag tggaactatc cgtattttag gtgaagaact
     2521 cactaagttt tcacccgaga agcgtgccaa gtacctgtct cgtgtcttcc aagatccaaa
     2581 gatggggaca gctccccgta tgacggtcgc tgaaaatctt ttaatcgcca agtttcgtgg
     2641 tgaaaagcgt ggattgttac cacgacgctt gactagctat aaggatgaat ttcaggcaac
     2701 cattgaaaaa gtaggaaatg gtcttgagaa acacttgaat acaccgattg agttcttatc
     2761 aggtggacaa agacaggctt tgagtctctt gatggcaacc ttgaagcgac ctgaattact
     2821 cctgttagat gagcatactg ctgccctgga tccaaagact agtgttgctt tgatggaatt
     2881 gacagatgaa tttgttaaga aagatcagct aacagccctt atgattactc atcatatgga
     2941 agatgctctc aaatacggca atcgcttgat tgtcatgaaa gaaggacgga ttatccaaga
     3001 tttaaaccaa gaagaaaaag caaaaatgaa aatctctgat tattatcaac tctttgaata
     3061 aagtagggaa aatgagtgaa atggtttact ttttttctga aataaagtat actatataaa
     3121 gtaaactatg ataacatgga ggtattgtgt atggttgaca aacaagtcat tgaagaaatc
     3181 aaaaacaatg ccaacattgt ggaagtcata ggagatgtga tttctttaca aaaggcagga
     3241 cggaactatc tagggctctg tccttttcat ggtgaaaaaa caccttcttt caacgttgta
     3301 gaggataagc agttttatca ctgtttcggt tgtggtcgct caggtgatgt ctttaagttc
     3361 atcgaggagt accaaggggt tccctttata gaggcagtcc aaatcttagg tcagcgtgtt
     3421 ggaattgagg ttgaaaaacc gctttatagt gagcagaagc cagcctcacc tcaccaagct
     3481 ctttatgata tgcacgaaga tgcagccaaa ttttaccatg cgattctcat gacaacgact
     3541 atgggcgaag aggccagaaa ttacctttat cagcggggtt tgacagatga agtgcttaaa
     3601 catttttgga ttggtttagc acctccagaa cgaaactatc tctatcaacg tttgtctgat
     3661 cagtatcgtg aagaggattt actggattca ggcctgtttt atctctcgga tgccaatcaa
     3721 tttgtagaca cctttcacaa tcgcattatg tttcccctga caaatgacca aggaaaggtc
     3781 attgccttct caggtcgtat ctggcaaaaa acggattcac aaacttctaa gtataaaaac
     3841 agccgatcga ctgcaatttt taacaaaagt tacgaattat atcatatgga tagggcaaaa
     3901 aaatcttctg gaaaagctag tgagatttac ttgatggaag gattcatgga tgttattgca
     3961 gcctatcggg ctggaatcga aagtgctgtg gcgtcgatgg gaacggcctt gagtcgagag
     4021 catgttgagc atctgaaaag gttaaccaag aaattggttc ttgtttacga tggagataag
     4081 gctgggcaag ccgcgacatt gaaagcattg gatgaaattg gtgatatgcc tgtgcaaatc
     4141 gtcagcatgc ctgataactt ggatcctgat gagtatctac aaaaaaatgg tccagaagac
     4201 ttggcctatc tattaacgaa aactcgtatt agtccgattg agttctacat tcatcagtac
     4261 aaacctgaaa acagtgaaaa tctgcaggct cagattgagt ttcttgaaaa aatagctccc
     4321 ttgattgttc aagaaaagtc catcgctgct caaaacagct atattcatat tttagctgac
     4381 agtctggcgt cctttgatta tacccagatt gagcagattg ttaatgagag tcgtcaggtg
     4441 caaaggcaga atcgcatgga aggaatttcc agaccgacgc caatcaccat gccaatcacc
     4501 aagcagttat cggctattat gagggcagaa gcccatctac tctatcggat gatggaatcc
     4561 cctcttgttt tgaacgatta ccgtttgcga gaagactttg catttgctac acctgaattt
     4621 caggtcttat atgacttgct tggccagtat ggaaatcttc ctccagaagt tttagcagag
     4681 cagacagagg aagttgaaag agcttggtac caagttttag ctcaggattt gcctgctgaa
     4741 atatcgccgc aggaacttag tgaagtagag atgactcgaa acaaggctct cttgaatcag
     4801 gacaatatga gaatcaaaaa gaaggtgcag gaagctagcc atgtaggaga tacagataca
     4861 gccctagaag aattggaacg tttaatttcc caaaagagaa gaatggagta ataatggcaa
     4921 caaaacaaaa agaagtaaca acatttgacg tacaggtagc agaatttatc cgtaatcata
     4981 agcaaaaagg gacagcaaca gatgatgaaa tcaatgcaag tctggttatt ccttttacct
     5041 tggacgctga tgggattgaa gatctcttgc aacggattca ggatgcagga atttctatca
     5101 cagataacga aggaaatcca agtgcgcgtg ttcttagcaa tgaagaagaa ccagaactca
     5161 gcgatgagga cttgattggg tcaacttctg ctaaggtcaa tgaccctgtc cgtatgtact
     5221 tgaaagaaat aggggtcgtt cctctcttga ccaatgaaga ggagaaagag ttggcactgg
     5281 ctgttgaagc tggtgatatc gaagccaaac aacgtcttgc ggaagccaat cttcgtttgg
     5341 ttgtttccat tgccaaacgc tatgtcggtc gtggcatgca gttccttgac ttgattcaag
     5401 aaggaaatat gggcttgatg aaggcggttg acaagtttga ctattctaaa gggttcaagt
     5461 tttcaactta tgcaacttgg tggattcgtc aggctatcac tcgtgctatt gcggaccaag
     5521 ctcgtaccat ccgtatccca gttcacatgg ttgaaactat caataaattg gttcgtgaac
     5581 agcggaatct ccttcaagaa ttggggcaag atccgacacc agaacagatt gctgaacgaa
     5641 tggatatgac acctgataag gttcgtgaaa tcttgaagat tgcccaagaa ccagtatctc
     5701 ttgaaactcc tatcggtgaa gaggacgata gccaccttgg agactttatc gaagatgaag
     5761 tgattgaaaa tccagtggat tatacgactc gtatcgtctt gcgtgagcaa ttggatgaaa
     5821 tcttagatac tcttacagac cgtgaagaaa atgttctgcg tctacgtttt ggactagatg
     5881 atggaaaaat gcgcacactt gaagatgtgg ggaaagtctt taacgtaact cgtgagcgta
     5941 tccgtcagat tgaagcaaag gctttgagaa aactacgcca accaagtcgt agcaaaccgc
     6001 ttcgtgattt tattgaagac taagagtgag gaaaatatgg cttatacaga agagcaaatt
     6061 gaaaacatca aaacacggat tttaacagcc ttggaagaag tcatcgaccc tgagttggga
     6121 atcgatattg tcaatcttgg tttgatctat gagattcgtt ttgacggtga cacagggcaa
     6181 acagagattg acatgacttt gacaactatg ggttgtccct tagcagacct tttgacagac
     6241 cagatttatg atgctatgat agaggttcca gaagtaacgg atactgaggt taaattagtc
     6301 tggtatccag cctggacagt tgaaaaaatg agtcgctatg cccgcattgc cctaggcatt
     6361 aagtaaacat ataaaagcat gtgagagact gttggaaaag cgaggaaatt tcccctcttt
     6421 tcctctagtc tctcctttct tttgctgatt ttattcaaag aaaatgatat aatagtagtt
     6481 atggagaaaa agaaattacg catcaatatg ttgagttcaa gtgagaaagt agcaggacag
     6541 ggagtttcag gtgcttaccg tgaattagtt cgtcttcttc accgtgctgc caaggaccaa
     6601 ttgattgtta cagaaaatct tccaatcgag gcagatgtga ctcactttca tacgattgat
     6661 tttccctatt atttatcaac cttccaaaag aaacgctcag ggagaaagat tggctatgtg
     6721 catttcttgc cagctacact tgagggaagt ttgaaaattc catttttctt aaagggaatt
     6781 gtgaaacgct atgtattttc tttttacaac cggatggagc acttggttgt ggtcaatcct
     6841 atgtttattg aggatttggt agcagctggt attccacgtg aaaaagtgac ctatattcct
     6901 aactttgtca acaaggaaaa atggcatcct ctaccacaag aagaggtagt cagactgcgc
     6961 acagatcttg gtcttagtga caatcagttt atcgtagtag gtgctgggca agttcagaaa
     7021 cgtaaaggga ttgatgactt tatccgtctg gctgaggaat tgcctcagat tacctttatc
     7081 tgggctggtg gcttctcttt tggtggtatg acagatggtt atgaacacta taagaaaatt
     7141 atggaaaatc cccctaaaaa tttgattttt ccaggcattg tatcgccaga gcggatgcgc
     7201 gaattgtatg ctctagcgga tcttttcttg ttgcctagtt acaatgagct ctttcctatg
     7261 actattttag aagctgcgag ttgtgaggct cctattatgt tgcgtgattt agatctctat
     7321 aaggtaattt tggagggaaa ttatcgggcg acagcgggta gagaagagat gaaagaggct
     7381 attttggaat atcaagcaaa tcctgctgcc ttaaaagatc tcaaagaaaa ggctaagaat
     7441 atttccagag agtattctga agagcatctg ttacaaatct ggttggactt ttatgagaaa
     7501 caagccgctt tagggagaaa gtaaaaagtg aggtaatcta tgcgaattgg tttatttaca
     7561 gatacctatt ttcctcaggt ttctggtgtt gcgaccagta ttcgaacctt gaaaacagaa
     7621 cttgaaaagc agggacatgc tgtttttatc tttacgacga cagataagga tgtcaatcgc
     7681 tacgaagatt ggcaaattat ccgcattcca agtgttcctt tctttgcttt taaggatcgt
     7741 cgctttgcct accgaggttt tagcaaggca cttgaaattg ctaaacagta tcagctagat
     7801 attatccata ctcagacaga attttctctt ggcctgttgg ggatttggat tgcgcgtgaa
     7861 ttgaaaattc cagtcatcca tacctatcac acccagtatg aagactatgt ccattatatt
     7921 gctaagggga tgttgatccg gccgagtatg gtcaagtatc tggttagagg tttcctgcat
     7981 gatgtggatg gggttatttg tcctagtgag attgtccgtg acttgctatc tgattataag
     8041 gtcaaggttg aaaaacgggt cattcctact gggattgaat tagccaagtt tgagcgtccg
     8101 gaaatcaagc aggaaaattt gaaagaactg cgtagtaaac tagggattca agatggtgaa
     8161 aagacgttgc ttagtctttc gagaatctcc tatgaaaaaa atattcaagc agttttagca
     8221 gcctttgctg atgttctgaa agaggaagac aaggttaaac tggtagtagc tggggatggc
     8281 ccttatctga atgacctcaa agagcaagct cagaacctag agattcaaga ctcagtcatc
     8341 tttacaggga tgattgctcc tagtgagacg gctctttact ataaagcggc ggatttcttc
     8401 atttcggcat cgacaagcga aacgcaaggt ttgacctact tggaaagctt agccagtgga
     8461 acacctgtca ttgctcacgg aaatccttat ttgaacaacc tcatcagtga taaaatgttt
     8521 ggaaccttgt actatggaga acatgatttg gctggtgcta ttttggaagc cctgattgca
     8581 acaccagaca tgaacgagca taccttatca gagaaattgt atgagatttc agctgagaac
     8641 tttgggaaac gagtgcatga gttttatctg gatgccatta tttcaaataa cttccagaaa
     8701 gatttggcta aagatgatac ggtcagtcag cgtatcttta agacagtttt gtatcttcag
     8761 caacaggtgg ttgctgtacc tgtaaaagga tctagacgca tgttgaaggc ttcaaaaaca
     8821 cagttgatca gtatgagaga ctattggaaa gaccatgaag aatagaaaga ggaacagcta
     8881 tgaaaaaaac aattaatgag aagcggtcgt gataaaaaga ttgcgggtgt ttgtgctggg
     8941 gtggcccatt atctggatat ggatccgact atcgttcaag tcatttgggg tgttcttact
     9001 tgctgttacg gagctggaat tgtagcttac attattttat ggattatcgc accagtagca
     9061 actgactatt gaaaactagc tcaattcatg ctaagataat agaaaataga atgtaacgaa
     9121 ggagagaaaa atggcatttg gagataatgg aaatcgtaaa aaaactatgt ttgagaaaat
     9181 aaccttgttt atcgtgatta tcatgctagt agcaagttta ttgggaattt ttgcaactgc
     9241 aattggtgcc ctcagtaatc tataaaatag attcaagaaa atttagtgac tgggatttcc
     9301 cagccctttt ttaaagtgag aagaaataat gagtatgttt ttagatacag ctaagattaa
     9361 ggtcaaggct ggtaatggtg gcgatggtat ggttgccttt cgtcgtgaaa aatatgtccc
     9421 taatggaggc ccttggggtg gtgatggtgg tcgtggaggc aatgtggtct tcgttgtaga
     9481 cgaaggacta cgtaccttga tggatttccg ctacaatcgt catttcaagg ctgattctgg
     9541 tgaaaaaggg atgaccaaag ggatgcatgg tcgtggtgct gaggacctta gagttcgagt
     9601 accacaaggt acgactgttc gtgatgcgga gactggcaag gttttaacag atttgattga
     9661 acatgggcaa gaatttatcg ttgcccacgg tggtcgtggt ggacgtggaa atattcgttt
     9721 cgcgacacca aaaaatcctg caccggaaat ctctgaaaat ggagaaccag gtcaggaacg
     9781 tgagttacaa ttggaactaa aaatcttggc agatgtcggt ttagtaggat tcccatctgt
     9841 agggaagtca acacttttaa gtgttattac ctcagctaag cctaaaattg gtgcctacca
     9901 ctttaccact attgtaccaa atttaggtat ggttcgcacc caatcaggtg aatcctttgc
     9961 agtagccgac ttgccaggtt tgattgaagg ggctagtcaa ggtgttggtt tgggaactca
    10021 gttcctccgt cacatcgagc gtacacgtgt tatccttcac atcattgata tgtcagctag
    10081 cgaaggccgt gatccatatg aggattacct agctatcaat aaagagctgg agtcttacaa
    10141 tcttcgcctc atggagcgtc cacagattat tgtagctaat aagatggaca tgcctgagag
    10201 tcaggaaaat cttgaagaat ttaagaaaaa attgtctgaa aattatgatg aatttgaaga
    10261 gttaccagct atcttcccaa tttctggatt gaccaagcaa ggtctggcaa cacttttaga
    10321 tgctacagct gaattgttag acaagacacc agaatttttg ctctacgacg agtccgatat
    10381 ggaagaagaa gcttactatg gatttgacga agaagaaaaa gcctttgaaa ttagtcgtga
    10441 tgacgatgcg acatgggtac tttctggtga aaaactcatg aaactcttta atatgaccaa
    10501 ctttgatcgt gatgaatctg tcatgaaatt tgcccgtcag cttcgtggta tgggggttga
    10561 tgaagccctt cgtgcgcgtg gagctaaaga tggggatttg gtccgcattg gtaaatttga
    10621 gtttgaattt gtagactagg agactggtat gggagataaa ccgatatctt tccgagatgc
    10681 ggatggtaat tttgtttccg ccgcagacgt ttggaatgaa aagaaattgg aagaactatt
    10741 taatcgtctc aatccaaatc gtgccttgag attggcacga actaaaaagg aaaatccatc
    10801 tcagtaaaga agctaaaaaa tcccgtgcct catcagacac gggattttgt ggtacgacag
    10861 gcatgtatag caaactgaat ctggaatagc acagcatatc ttctaaaata ttgctataaa
    10921 ttgatttgaa tttcaaaatt aaattgtttt attcttattt caatttgtta tagtatatct
    10981 gatgtcaaag ttctcggcga gtcaaatagt gattcccaag cctgactatc gtgaggtagc
    11041 ggattaaaat ggtctgggga tagaccgttt taagtctgat gctggaaata agaattgtca
    11101 gaagaaggga tagcgaaatc gtggctctac gaacaggaac gtgataataa ggcgtatata
    11161 gcgaataaga gggcctcaaa ctctaaagtc caaaaaggta gtcgtaacct atatgcgtaa
    11221 atcatgagag taattgaatt cgtactaaga ttttctattt ttactgtaac cttttaacgt
    11281 cctcatatct tgtatacacg aggaaagatg tacgacttat cccgtgaggt ctatcactat
    11341 aaagagaaaa cgacagatag aagtgatcct gagtcacggt tatctttaaa atagattgtc
    11401 tgataggatg ctagcctgaa aacgtcttgt tcatgaaata taggacggta tgtataaaac
    11461 gcttctgtga actgagagaa gggggagaag ttcttgctaa aatttagttg aacagccgta
    11521 ttccgatact tagataagag atctagtctt agctcctact cagtttcagg ggataaaaaa
    11581 ggggcaatag cgattcgaga aagattatac tcttcgaaaa tctcttcaaa tcacgtcaat
    11641 atcgccttgc cgtatgtgta ggatactaac tacgtcagtt ccatctacaa tctcaaaaca
    11701 gtgttttgag caacctgcgg ctagtttcct agtttgatct ttgattttta ttgagtatta
    11761 gtaattcagt tactaactcg tcaactctga tttatccaat aaaattgaaa aggatggaaa
    11821 aaaggataaa tttatgatat actttatttt gaagacctta ttagaaatct tgaaagagta
    11881 ttgaaaactt agaatgagaa aaattgttat caatggtgga ttaccactgc aaggtgaaat
    11941 tactattagt ggtgctaaaa atagtgttgt ggccttaatt ccagctatta tattggctga
    12001 tgatgtggtg actttggatt gtgttccaga tatttcggat gtagccagtc ttgtcgaaat
    12061 catggaatta atgggagcta ctgttaagcg ttatgacgat gtcttggaga ttgatccaag
    12121 aggtgttcaa aatattccaa tgccttatgg taaaattaac agtcttcgtg catcttacta
    12181 tttttatggg agcctcttag gccgttttgg tgaagcgaca gttggtctac cgggaggatg
    12241 tgatcttggt cctcgtccga ttgacttaca ccttaaggcg tttgaagcta tgggtgccac
    12301 tgctagctac gagggagata acatgaagtt atctgctaaa gatacaggac ttcatggtgc
    12361 aagtatttac atggatacgg ttagtgtggg agcaacgatt aatacgatga ttgctgcggt
    12421 taaagcaaat ggtcgtacta ttattgaaaa tgcagcccgt gaacctgaga ttattgatgt
    12481 agctactctc ttgaataata tgggtgccca tatccgtggg gcaggaacta atatcatcat
    12541 tattgatggt gttgaaagat tacatgggac acgtcatcag gtgattccag accgcattga
    12601 agctggaaca tatatatctt tagctgctgc agttggtaaa ggaattcgta taaataatgt
    12661 tctttacgaa cacctggaag ggtttattgc taagttggaa gaaatgggag tgagaatgac
    12721 tgtatctgaa gacagcattt ttgtcgagga acagtctaat ttgaaagcaa tcaatattaa
    12781 gacagctcct tacccaggct ttgcaactga tttgcaacaa ccgcttaccc ctcttttact
    12841 aagagcgaat ggtcgtggta caattgtcga tacgatttac gaaaaacgtg taaatcatgt
    12901 ttttgaacta gcaaagatgg atgcggatat ttcgacaaca aatggtcata ttttgtacac
    12961 gggtggacgt gatttacgtg gggccagtgt taaagcgacc gacttaagag ctggggctgc
    13021 actagtcatt gctgggctta tggctgaagg taaaactgaa attaccaata tcgagtttat
    13081 cttacgtggt tattctgata ttatcgaaaa attacgtaat ttaggagcgg atattagact
    13141 tgttgaggat taaaccgtag aggtgtttat gaatatttgg accaaattag caatgttttc
    13201 tttttttgaa acggatcgct tgtatttgcg tcctttcttt tttagtgata gtcaggactt
    13261 ccgcgagata gcttcaaatc cagaaaatct tcaatttatt ttcccaacgc aggcaagtct
    13321 ggaagaaagt caatatgcac tggccaatta ctttatgaag tcccctttgg gagtgtgggc
    13381 aatttgtgac cagaaaaatc aacaaatgat tggttctatt aaatttgaga agttagatga
    13441 aatcaaaaaa gaagctgagc ttggctattt tttgagaaaa gatgcttggt cgcaaggatt
    13501 tatgacagag gttgttagaa aaatttgtca gctttctttt gaggaatttg gcttaaaaca
    13561 attatttatc attacccacc ttgaaaatga agctagccaa agagttgctc ttaagtctgg
    13621 atttagtttg ttccgtcagt ttaagggaag tgatcgttac acaagaaaaa tgcgggatta
    13681 tcttgaattt cggtatgtaa aaggagagtt caatgagtaa gcatcaggaa attctaagct
    13741 atttggagga attaccagta ggtaaaaggg tcagtgttcg tagcatttcg aatcatctag
    13801 gagttagtga tggaacagcc tatcgagcta ttaaagaagc tgaaaaccgt ggaattgtgg
    13861 agacccgtcc tagaagtgga acaattcgtg ttaaatccca gaaagttgct atagagagat
    13921 taacgtttgc tgaaattgca gaagtgactt cttctgaggt tctggctggg caagaaggtt
    13981 tagagagaga gtttagtaag ttttcaattg gtgccatgac tgaacaaaat atcttgtctt
    14041 accttcatga tggggggctc ttgattgtcg gagaccgaac ccgtattcag ttgctagcct
    14101 tggaaaatga aaatgcagtt ctggttacag ggggatttca ggttcatgat gatgtgctta
    14161 aactggccaa tcaaaaaggg attcctgttc taagaagtaa gcatgatacc tttaccgtcg
    14221 cgaccatgat caataaagcc ttgtcaaatg tccaaatcaa gactgatatt ctgacagttg
    14281 agaaacttta tcgccctagt catgagtatg gttttctgag agagacagat acagttaaag
    14341 attatttgga cttggttcgt aagaatcgta gcagccgttt ccctgttatc aatcaacatc
    14401 aggtcgttgt tggtgttgta accatgagag acgctggtga taaatcacca agcacgacaa
    14461 ttgataaggt tatgtctcgt agtctatttt gggttggatt atcgacaaat attgccaatg
    14521 tgagtcaacg gatgatcgca gaagactttg aaatggtacc agttgttcga agcaatcaaa
    14581 ctttgcttgg cgttgtgacg cgacgagatg tcatggagaa gatgagccgt tcccaagttt
    14641 cggctctacc aactttttct gagcagattg gacaaaagct ctcttatcac catgatgaag
    14701 tagtcattac agtggaaccc tttatgctag aaaaaaatgg agttttggct aatggtgtat
    14761 tggcagaaat tctgacccac atgacccaag atttagttgt taatagtggt cgcaatctca
    14821 ttatcgagca gatgctgatc tactttttgc aggctgttca gatagatgat atattgcgca
    14881 ttcaggcacg gattattcat catacgagac ggtcagctat aattgattac gatatttatc
    14941 atggtcacca gattgtttca aaagcaaatg tgactgttaa aattaattag aaactaggag
    15001 aaaagatgat aacattaaaa tcagctcgtg aaatcgaagc tatggacaag gctggtgatt
    15061 ttctagcaag tattcatata ggcttacgtg atttgattaa gccaggcgta gatatgtggg
    15121 aagttgaaga atatgtccgc cgtcgttgta aagaagaaaa tttccttcca cttcagattg
    15181 gggttgacgg tgccatgatg gactatcctt atgctacctg ttgctctctt aacgatgaag
    15241 tggctcacgc tttccctcgt cattatatct tgaaagatgg tgatttgctc aaagttgata
    15301 tggttttggg aggtcccatt gctaaatctg acctaaatgt ctcaaaatta aacttcaaca
    15361 atgttgaaca aatgaaaaaa tacactcaga gctattctgg tggtttagca gactcatgtt
    15421 gggcttatgc tgttggtaca ccgtccgaag aagtcaaaaa cttgatggat gtaaccaaag
    15481 aagctatgta caagggtatt gagcaagctg ttgttggaaa tcgtatcggt gatatcggtg
    15541 cggctattca agaatacgct gaaagtcgtg gttacggtgt agtgcgtgat ttggttggtc
    15601 atggtgttgg cccaactatg cacgaagaac caatggttcc taactatggt attgcaggtc
    15661 gtggactccg tcttcgtgaa ggaatggtct taaccattga accaatgatc aatacaggcg
    15721 attgggaaat tgatacagat atgaaaactg gttgggcgca taagaccatt gacggtggat
    15781 tgtcatgtca gtatgaacac caatttgtca ttacgaaaga tggacctgtt atcttgacta
    15841 gccaaggtga agaaggaact tattaataaa aagtgaaaag actactggaa gtttattttg
    15901 ataaaaaatc cagtagatct tttcataata aaacgcattg tatcaagtgt taggggctga
    15961 tatcatgcgt ttttctgctt ttaagatttt ttccaactct gtttgtaagc gcatcataac
    16021 aaagggtcta ggattcaggg ctctcctcct atatactatt agtaaagtaa aactaaggga
    16081 ggatatttta gtgtcgcagt ctattgttcc tgtagagatt ccacaatatt gtcgttttga
    16141 ttctaaaaag agaaatggaa ttctgtttaa cgttcgtatt gccaatctta aatttacctt
    16201 ttttagatta gacttcctgc gaaataaaat atggtatagt agttctatga atgatgaagc
    16261 aagtaaacaa ctaactgatg caggatttaa gcgtcttgtt tgtgttcagc gtaccacttt
    16321 tgaagagatg ttagctgtat taaaaacagc ttatcaactt aaacacgcaa aaggtggacg
    16381 aaaacctaaa ttaagcctag aagaccttct tatggccact cttcaatatg tgcgagaata
    16441 tcgaacttat gaagaaattg cgactgattt tggtatccac gaaagcaact taatccgtcg
    16501 gagccaatgg gttgaagtaa ctcttgttca aagtggtgtt acgatttcaa gaactcctct
    16561 cagctctgag gacacggtaa tgattattga tgcgacggaa gtaaaaatca atcgccctaa
    16621 aaaaacaatt agcgaatgat tctggtaaaa agaaatgcta cgctatgaag gctcaagcga
    16681 ttgtcacaag tcaagggaga ttgtttcttt ggatatcact gtgaactatt gtcatgatat
    16741 gaagttgttc aaaatgagtc gcagaaatat cggacaagct ggtaaaatct tggctgacag
    16801 tggttatcaa gggctcatga agatatatcc tcaagcacaa actccacgta aatccagcaa
    16861 attcaagccg ctaacagttg aagataaaac ctataaccat gcgctatcta aggagagaag
    16921 caaggttgag aacatctttg ccaaagtaga aacgtttaaa atgttttcaa caacctatcg
    16981 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gctggtatta tcaatcatga
    17041 actaggattc tagttttgca ggaagtctat tgaggtattg agctagttta tgaaaaaatt
    17101 gggtgaaaag tcgagtgttt tagaaaccca cagtgtagta ttctagtttc aatccactat
    17161 attttgctac tccccgtaaa gtttctattt tccctgattt ctgatataat agaaatattg
    17221 acttcaagag taaggaagag aagatgaacg cattattaaa tggaatgaat gaccgtcagg
    17281 ctgaggcggt gcaaacgaca gaaggtccct tgctaatcat ggcaggggct ggttctggaa
    17341 agactcgtgt tttaacccac cgtatcgctt atttgattga tgaaaagctg gtcaatcctt
    17401 ggaatatctt ggccattacc tttaccaaca aggctgcgcg tgagatgaaa gagcgtgctt
    17461 atagcctcaa tccagctact caggactgtc tgattgcgac cttccactcc atgtgtgtgc
    17521 gtattttgcg tcgcgatgcg gaccatattg gctacaatcg taattttaca attgtggatc
    17581 ctggtgaaca gcgaacgctc atgaaacgta ttctcaaaca gttgaacttg gatcctaaaa
    17641 aatggaatga acgaactatt ttggggacca tttccaatgc taagaatgat ttgattgatg
    17701 atgttgctta tgctgcccaa gctggcgata tgtatacgca aattgtggcc cagtgttata
    17761 cagcctatca aaaagaactt cgtcagtctg aatccgttga ctttgatgat ttgattatgc
    17821 tgaccttgcg tctctttgat caaaatcctg atgttttaac ctactaccag caaaaattcc
    17881 aatacatcca cgttgatgag taccaagata ccaaccacgc tcagtaccaa ttggtcaaac
    17941 tcttggcttc ccgttttaaa aatatctgtg tggttgggga tgcggaccag tctatctacg
    18001 gttggcgtgg tgctgatatg cagaatatct tggactttga aaaggattac cccaaagcca
    18061 aggttgtttt gttggaggaa aattaccgct caaccaaaac cattctccaa gcggccaacg
    18121 aggttattaa aaacaataaa aatcgccgtc ctaaaaatct ctggactcaa aacgctgatg
    18181 gggagcaaat cgtttactat cgtgccgatg atgagctgga tgaggctgta tttgtagcca
    18241 gaaccatcga tgaacttagt cgcagtcaaa acttccttca taaggatttt gcagttctct
    18301 atcggactaa tgcccagtcc cgtacaattg aggaagccct gctcaagtct aacattcctt
    18361 ataccatggt tggcggaacc aaattctaca gccgtaagga aattcgcgat attattgctt
    18421 atctcaacct tattgctaat ttgagtgaca atattagttt tgagcgtatt atcaacgagc
    18481 ctaaacgtgg aattggtcca ggtacagttg agaaaatccg tgattttgca aatttgcaaa
    18541 atatgtctat gctggatgct tctgctaata ttatgttgtc tggtatcaag ggtaaggcag
    18601 cccaatctat ctgggatttt gccaatatga tgcttgattt gcgggagcag ctagaccact
    18661 taagcattac agagttggtt gagtccgtcc tagaaaaaac aggttatgtc gatattctta
    18721 acgcccaagc gactctagaa agcaaggcac gggttgaaaa tatcgaagag tttctttctg
    18781 ttacgaagaa ctttgatgac accactgatg tgacagaaga ggaaactggt ctggacaaac
    18841 tgagtcgttt cttaaatgac ttggctttga ttgccgacac agattcaggt agtcaggaga
    18901 catcagaagt gaccttgatg accctgcatg ctgccaaagg tctcgaattt ccagttgtct
    18961 ttttgattgg gatggaagaa aatgtctttc cacttagtcg tgcgactgaa gatccagatg
    19021 aattagaaga agagcgccgt ctagcctatg taggtatcac gcgtgcagag aaaattctct
    19081 atctgaccaa tgccaactca cgcttgcttt ttggtcgtac caattataac cgtccgactc
    19141 gttttattaa cgaaatcagt tcagacttgc ttgagtatca aggtctggct cgtcctgcaa
    19201 atacaagctt taaggcatca tatagcagtg gtagtatttc ctttggtcaa ggtatgagtt
    19261 tggctcaggc tcttcaagac cgtaaacgcg gtgctgcccc aaaatcaatc cagtcaagcg
    19321 gtcttccatt tggtcaattt acagctggcg caaaatcagc atctagcgag gcaaattggt
    19381 ccattggtga cattgctctc cacaagaaat ggggagaggg aactgttctg gaagtttcag
    19441 gtagcggtgc taggcaggaa ttgaaaatca atttcccaga agtaggtttg aaaaaacttt
    19501 tagccagtgt ggctccaatt gagaaaaaaa tctaattttc catccttctc acgaataata
    19561 aagtgaggag gatttttatg tacagtattt cattccaaga agattcacta ttaccaagag
    19621 aaaggctggc caaggaagga gttgaagcgc ttagtaacca agagttgcta gctattttac
    19681 tcaggacagg aacacgtcaa gctagcgttt ttgaaattgc ccaaaaagtc ttgaacaatc
    19741 tttcaagcct aacggatttg aaaaaaatga ccctgcagga attgcagagt ttgtctggta
    19801 ttgggcgtgt taaggccata gaattacaag ctatgattga actggggcat cgtattcaca
    19861 aacacgagac tcttgaaatg gaaagtattc tcagcagtca aaagttggcc aagaagatgc
    19921 agcaggaatt aggggataaa aaacaagagc acctggtggc actctatctc aatactcaaa
    19981 atcaaatcat ccatcagcag accattttta tcggttctgt aactcgtagt atcgctgaac
    20041 cgcgagagat tcttcactat gcaatcaagc atatggcgac ttctcttatc ttggtccaca
    20101 atcatccttc aggagcggta gcgcctagcc aaaatgatga tcatgtcact aaacttgtta
    20161 aagaagcctg cgaactgatg gggattgttc tcttggacca tttgattgtc tctcattcta
    20221 attactttag ttatcgtgaa aagacagatt taatctaaag ttcattaacg acatagtcaa
    20281 agagtttctt atctttggga cgattttcaa aaagaagttc tggatgccat tggacaccga
    20341 gaaaggcgac atcatccgta ctcatgacag cctcaatgat accatcttta ggatcatgag
    20401 ccacaacttt taaatttggt gctaagtcct tgatgctctg gtggtggaag gagttgatat
    20461 gagagatttc tccatagatt tcttggagaa cggtatctgg ttctgttacc aagcgttgag
    20521 ttgtgtactc aacagaagaa tcctgccaat ggtcttcgat atcttggtac aaagttccac
    20581 ccatggcaac gttaaagagt tgggtaccac ggcagacaga gaaaatgggc tttttctgtt
    20641 taatagcttc cttgatgagg gccagttcga agatatctct ttgaaggtga taatcatcgc
    20701 tatcaatggt tttgggttcg ccatagaatt ttggatcgac attttgccca cctgtcaaga
    20761 tgagcttgtc aatcaaactg atatagtggc aggccatttc ttgatcacca atcggtagga
    20821 tgataggaat ccctccagca tctttaacgc cttcaacaaa gccttttgct gcgtagctca
    20881 tcatgatgtc atcatctgga tgagtttttt cgtttcctgt aatcccaata actggttttt
    20941 tcataaaatg attttcgctt tctaatcctc ttttcgcatg aagtagagga gggtttggag
    21001 ttcacttgtc aaatcgacat actgaacgac cacgtctttt ggtaaatgca gatggactgg
    21061 tgaaaaactg agaattcctt tcacaccagc atcaaccaag agattagcaa cctcttgtga
    21121 cttgacgctg ggaacagtta ggatagcagt cttcacatca gcatccttga ttttatcctt
    21181 gatctgagaa atcccgtaaa tgggaatccc gtcaggagtt tgggtaccga cttcaggatg
    21241 gtcgtctagg tcaaaggcca tgataatctt catcttgtta cgttcgtgga agcggtagtg
    21301 gagaagggca tggcccatat ttccaatacc aaccagcatg acattggtaa tagagttgtc
    21361 attgagcaaa tcggcaaaaa atgtcattag ttttttgaca tcatagccaa aaccacgacg
    21421 accaagttca ccaaaatagg aaaaatcacg acgtacggtc gctgaatcaa taccgatagc
    21481 ctctgcaatt tgcttagagt tggcacgttc aatcttttct gcatgaaatc tcttaaaaat
    21541 tcgatagtag agagagagtc tttttgctgt agcttttgga atagcaaact gtttatcttt
    21601 cacaaaatca caacctttct attcttctat tttatagaaa cattgtgaaa aaatcaacaa
    21661 aaataagaaa aaactaagaa aaatcttagt tttgatgtaa aaaatctgca tgagatagaa
    21721 aacggtagag gtctccgacc agaccctgat aaactttttg gcccctaaaa gtcagagaag
    21781 tcacataaag tgtatctggt aaggttacac atcctgacaa agtcaacatg agagcctcat
    21841 gatcctcata cttgagagta cgctctacat gatagcagtc cttataggtc agttcaaaca
    21901 ttttggctct atctttccga ttttgtaaag acaccacgtt ctaccaagct atccatgagg
    21961 aagtagaatt tttcctgatg aatatggtgg tcttctgatt tgaaaatatc aactagacga
    22021 aggccaaact tgtcagtgat attgatttta gcccctgtaa gttccttgtt aatgatgatt
    22081 ttgagttgga agccttcacc gctgtttggc actttttcca aaaggcgagt cagttcatag
    22141 ttaccaacct tagtttcaaa aaaggtgtta tctttgaggg tgaatttttt aacagaaggg
    22201 ctaagagtgt aatcgtaacg acaatttttt aactgaatga ttttttcaaa tgccatatgg
    22261 ctaacctccg ataatttctt ttaaggtttt tgcgagggtt tgtaggtctt caacggtatt
    22321 ttgtggcgac aaactgatgc gaagggattc cttcaagcgt tctgaatttg cgccatacat
    22381 ggcttcaaga acatggctgg attggacaac gcctgcagta caggctgagc cagtagagat
    22441 tgaaattcca gctaaatcta gccgaaggag taagaggtca tttttctgac caggaaatcc
    22501 aatattgaga acataaggga gatgatgttt tcctctattc aagtaatact gaatgccctc
    22561 cagctctgcc agaaaggcag tttctagatt ttgtacatgt tgaaaatgtt cttcttgttt
    22621 ttctaggtct tcttttaggg ctgcaaccat gcctacgata gcaggcagat tttcagttcc
    22681 tgcacgtttt ttctgttcct ggtctccgcc atgtagatag gaatcaaagt ccatgctaga
    22741 tgcgtagaga aaaccgattc ccttaggacc atggaatttg tgggcagaag cagtgagaaa
    22801 atcaatgccc aattcttctg aatgaattgg gattttacca atagcctgaa ctgcatcaac
    22861 atgataggca gcagggtgtt gcttgagtat ttggccaatt tcagcgatgg gcagtaggtt
    22921 tcctgtctca ttattggcaa acatggtaga aaccaaaatc gtatcgtcac gtaaagcctt
    22981 ttgaatttgc tgggctgtga tttcttgatt ttctggctgg ataatggttg cttcaaaccc
    23041 aaagtgttga accaagtaat caattgtttc aaggacagca tggtgctcga tggcagttgt
    23101 gatgatatgt tttccttgtt cttggtgacg aagacagtag ccaatgatgg tagtattatt
    23161 gccttcagtc ccaccagaag tgaaaaagat atgttgaggt tttgtcctta gtaactgggc
    23221 tagttcctga cgggcttctc gcaagagttt gccagcttga cgaccatgac catgaatact
    23281 agaaggattt ccgtgggttt cttgcataac cttggtcata gctgaaatag caactgctga
    23341 cataggagtc gttgcagcat tgtccaaata aatcaaagaa tcaccttatt tctttttatt
    23401 gtaggcaaag agtgggctga ctggttttct ttcgtgaata cggacgatag catcaccaat
    23461 taactcacta gcagtgatgt agcatacatt tttaggagtt ttttcttttg ttgctactga
    23521 atcagtcaca agaatttctt taatattagt attgtcaaga agctcagcag ctccctcgac
    23581 gaagagaccg tggctagaaa cagcataaat ttctgtagct ccttcacgtt caacgatttt
    23641 agaagcttca gagaaggtac gtcctgtatt taaaatatca tcaatcaaga tagctttctt
    23701 accttcaaca tcaccaataa tataaccttc gttacgagtt gcatcgtctt gagggtagtc
    23761 gataatggcg ataggagcat caagatattc agccaggcta cgcgcacgtt tgacacctga
    23821 atttttaggg ctaacgacaa caacatctga accaagcaat cctttatcgc agtaatgttt
    23881 tgcgaatagg ggaacagtga aaagattatc cactggaata tcaaagaaac cttgaacctg
    23941 aacggcatgc aaatcaagag tcaggatacg atcaactcca gccttaacca gcatattggc
    24001 aactagtttt gctgtaagtg gctcacgagg acaagcaatg cggtcttgac gtgcatagcc
    24061 aaaatatgga aggacaacgt tgatactgtg ggcacttgca cgcacacaag catcgaccat
    24121 gattaacaat tccattaggt ggttgttgac agggaaactt gttgattgga tgatgtaaac
    24181 atcataacca cggacacttt cttcgatatt tacttggatt tctccgtctg aaaattgacg
    24241 tgatgatagt tttccaagtg ggacaccaac agcttgggca attttttgtg caatctcttg
    24301 gttagagttg agtgcgaaaa gtttcatgtt ttttctatct gacattatag accgtcctct
    24361 gtaaacttta taaatcctag ttatatttac cttacatata tgaactggga tttgtgtatt
    24421 tttatctttt ctattttacc aaaaaatgga gattatttca gctatttttc atacttttga
    24481 caaatcgaac caattttgaa ggagcttttt ggtaggaaat ctgatttttc tctaaaaatt
    24541 gtcggaaatc ctgtttgcct tgctcatgat tttccacttc aagctccaat tcgtaatctg
    24601 ttatatcaaa gtatcggctc tgatccagtg ccatgagacc aatagctgtt ttcatttcat
    24661 agcgaagcgt tgttagacaa ccaagaacct gccagttctt actttggata ccatgtttcg
    24721 ccaattcatc cagtactagc ccttgaggaa gttcttcctt actcagatag ttctcagcat
    24781 cttttagttg caatttttgg ttgtattcca tgtttccaac actctgcggg actttgagtg
    24841 tcaactcagc ccagtcttca aaggttcgaa tgcgcatagc gactttcttt tctcgcagtt
    24901 caaaatcagg cgtgtcgatg tagtaatttg tttgaagaac aggagtgaca cctgtgaact
    24961 ggtcttttag acgattgtat tcatcttttt tcaatagtgt tttcaattca atttctaaat
    25021 gtttcatttt tcttaccttt ttttatcgtt gaaagcggat ttatggtata ataagcattg
    25081 tatttattgt atatgaatct ggagaaaaaa tcaaagatat ttttgacgga taatatgaga
    25141 acaagggaga atatatgacc ttagaatggg aagaatttct agatccttac attcaagctg
    25201 ttggtgagtt aaagattaaa cttcgtggta ttcgtaagca atatcgtaag caaaataagc
    25261 attctccaat tgagtttgtg accggtcgag ttaagccaat tgagagcatc aaagaaaaaa
    25321 tggctcgtcg tggcattact tatgcgacct tggaacacga tttgcaggat attgctggct
    25381 tacgtgtgat ggttcagttt gtagatgacg tcaaggaagt agtggatatt ttgcacaagc
    25441 gtcaggatat gcgaatcata caggagcgag attacattac tcatagaaaa gcatcaggct
    25501 atcgttccta tcatgtggta gtagaatata cggttgatac catcaatgga gttaagacta
    25561 ttttggcaga aattcaaatt cgtactttgg ccatgaattt ctgggcaacg atagaacatt
    25621 ctctcaacta caagtaccaa ggggatttcc cagatgagat taagaagcga ctggaaatta
    25681 cagctagaat cgcccatcag ttggatgaag aaatgggtga aattcgtgat gatatccaag
    25741 aagctcaggc actttttgat cctttgagta gaaaattaaa tgacggtgta ggaaacagtg
    25801 acgatacaga tgaagaatac aggtaaacga attgatctga tagccaatag aaaaccgcag
    25861 agtcaaaggg ttttgtatga attgcgagat cgtttgaaga gaaatcagtt tatactcaat
    25921 gataccaatc cggatattgt catttccatt ggcggggatg gtatgctctt gtcggccttt
    25981 cataagtacg aaaatcagct tgagaaggtc cgctttatcg gtcttcacac tggacatttg
    26041 ggcttctata cagattatcg tgattttgag ttggacaagc tagtgactaa tttgcagcta
    26101 gatactgggg caagggtttc ttaccctgtt ctgaatgtga aggtctttct tgaaaatggt
    26161 gaagttaaga ttttcagagc actcaacgaa gccagcatcc gcaggtctga tcgaaccatg
    26221 gtggcagata ttgtaataaa tggtgttccc tttgaacgtt ttcgtggaga cgggctaaca
    26281 gtttcgacac cgactggtag tactgcctat aacaagtctc ttggcggtgc tgttttacac
    26341 cctaccattg aagctttgca attaacggaa attgccagcc ttaataatcg tgtctatcga
    26401 acactgggct cttccattat tgtgcctaag aaggataaga ttgaacttat tccaacaaga
    26461 aacgattatc atactatttc ggttgacaat agcgtttatt ctttccgtaa tattgagcgt
    26521 attgagtatc aaatcgccca tcataagatt cactttgtcg cgactcctag ccataccagt
    26581 ttctggaacc gtgttaagga tgcctttatc ggtgaggtgg atgaatgagg tttgaattta
    26641 tcgcagatga acatgtcaag gttaagacct tcttaaaaaa gcacgaggtt tctaagggat
    26701 tgctggccaa gattaagttt cgaggtggag ctattctggt caataatcaa ccgcaaaatg
    26761 caacgtatct attggacgtt ggagactacg ttaccattga cattcccgct gagaaaggct
    26821 ttgaaacctt ggaggctatt gagcttccat tagatattct ctatgaggat gaccactttc
    26881 tagtcttgaa taaaccctat ggagtggctt ctattcctag tgttaatcac tctaatacca
    26941 ttgccaattt tatcaagggt tactatgtca agcaaaatta tgaaaatcag caggttcaca
    27001 ttgttaccag actagatagg gacacttctg gcttgatgct ctttgccaag cacggttatg
    27061 cccatgcacg attagacaag cagttgcaga agaaatctat cgagaaacgc tactttgctt
    27121 tggttaaggg agatggacat ttggaaccag aaggggaaat tattgctccg attgcgcgtg
    27181 atgaagattc cattattacc agacgagtgg ctaaaggcgg aaagtatgcc catacttcat
    27241 acaagattgt agcttcttat ggaaatattc acttggtcta tattcacctg cacactggtc
    27301 gaacccatca aatccgagtc catttttctc atatcggttt tcctttgctg ggagatgatt
    27361 tgtatggtgg tagtctggaa gatggtattc aacgtcaggc tctgcattgc cattacctat
    27421 ccttttatca tccattttta gagcaagact tgcagttaga aagtcccttg ccggatgatt
    27481 tcagtaacct tattacccag ttatcaacta atactctata aaaactgtct cagagtataa
    27541 ttattatctt aaaggagaaa actcatggaa gtttttgaaa gtctcaaagc caaccttgtt
    27601 ggtaaaaatg ctcgtatcgt tctccctgaa ggggaagagc ctcgtattct tcaagcaaca
    27661 aaacgcttag taaaagaaac agaagtgatt cctgttttgc ttggaaatcc tgaaaaaatt
    27721 aaaatttatc ttgaaattga aggaatcatg gatggttatg aggtcatcga ccctcaacat
    27781 tatcctcaat ttgaagaaat ggtttctgcc ttggtggagc gtcgcaaggg caaaatgact
    27841 gaagaagatg tacgcaaggt tttggttgaa gatgtcaact actttggtgt gatgttggtt
    27901 tacttgggct tggttgatgg aatggtgtca ggagcgattc actcaacagc ttcaacagtt
    27961 cgcccagctc tacaaatcat caaaactcgt ccaaatgtaa ctcgtacttc aggagccttc
    28021 ctcatggttc gtggtacgga acgttaccta tttggagact gtgccattaa tatcaatcca
    28081 gatgcagaag ccttggctga aattgccatc aactcagcaa tcacagctaa gatgtttggc
    28141 atcgaaccta aaattgccat gttgagctat tctactaaag gttcagggtt tggtgaaagc
    28201 gttgataagg tcgttgaagc aactaaaatt gctcacgact tgcgtcctga ccttgaaatc
    28261 gatggtgagt tgcaatttga tgcggccttt gttcccgaaa ctgcagctct gaaagctccg
    28321 ggaagtacag tagctggtca agcaaatgtc ttcatcttcc caggtatcga ggcaggaaat
    28381 atcggttaca agatggctga acgcctgggt ggctttgcgg ctgtaggacc tgttttgcaa
    28441 ggtttaaaca agccagttaa tgatctttct cgtggatgta atgcagatga tgtttacaag
    28501 ttgaccctca tcacagcagc tcaagcagtt catcaatagt gaaaactata aagtgatata
    28561 ctatgctata ctgtagttat gaaactatgt acgaaaagca ctgccattaa ttcctgagaa
    28621 ctaaattact gattggtgtc aaaaaggaaa acttccaagc gatgatatcc tgtctataca
    28681 cgacctatag aaatctgtaa tatacatatc cgtaaaacga taaattccct ttttgatttt
    28741 aaatgagtat gaaaagagaa ttttttggct ctttgtcaac tgtagtgggt tgaagtcagc
    28801 taagctcgag aaaggacaaa ttttgtcctt tcttttttga tattcagagc gataaaaatc
    28861 cgttttttga agttttcaaa gttccgaaaa ccaaaggcat tgcgcttgat aagtttgatg
    28921 agattattgg tcgcttccaa tttggcgttt gaatagggta gttgaagggc gttgacgatt
    28981 ttctctttgt cctttagaaa ggttttaaag acagtctgaa aaataggatg aacctgctta
    29041 agattgtcct caataagtcc gaaaaatttc tctggttcct tattctgaaa gtgaaacagc
    29101 aagagttgat agagctgata gtgatgtttc aagtcttgtg aatagctcaa aagcttgtct
    29161 aaaatctctt tattggttaa atgcatacga aaagtagggc gataaaaatg tttatcgctg
    29221 agtttacggc tatcctgttg aatgagtttc cagtagcgct tgatagcctt gtattcatgg
    29281 gattttcgat gaaactgatt catgatttgg acacgcacac gactcatagc acggctaaga
    29341 tgttgtacaa tatgaaagcg atcaagaacg attttagcat tcgggagtga aacagtctgg
    29401 gagactgttt cagcctgagc ctagaaattt gaaagcgaag ctgtttagcc aagtcatagt
    29461 aaggactaaa catatccata gtaataattt tgacgcgaca tcggacaact ctatcgtagc
    29521 gaagaaagtg atttcgaatg atagcttgtg ttctaccctc aagaacagtg ataatatcga
    29581 gcttttcaaa atcttgcgca atgaagctca tctccatctc ccgattgaaa cagtcactcc
    29641 ccggactgtt tcaacctccc aagacataat ctcaggcaga tttctaaaat tacactcaaa
    29701 gtggaagtca ttgagcttgc gaatgacagt tgaagttgaa atggccagct gatgggcaat
    29761 atcagtcata gaaatttttt caattaactt ttgagcaatt ttttggttga tgatacgagg
    29821 gatttggtga tttttcttta ccaggggagt ctcagcaacc atcatttttg aacagtgata
    29881 gcacttg
//
LOCUS       DAYYQN010000020        29209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYYQN010000020 DAYYQN010000000
VERSION     DAYYQN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.020"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..999
                     /locus_tag="ACHBRQ_000848"
     CDS             <1..999
                     /locus_tag="ACHBRQ_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9592550.1"
                     /translation="RRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMT
                     DIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNII
                     TVLEGRTQAVIRDHFLKYDRAVRCRVKIITMDMFSPYYGLAKQLRFHIVQHLSRAMSR
                     VRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLS
                     YSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVN
                     ALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(1099..2721)
                     /gene="groL"
                     /locus_tag="ACHBRQ_000849"
     CDS             complement(1099..2721)
                     /gene="groL"
                     /locus_tag="ACHBRQ_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9592551.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2737..3021)
                     /gene="groES"
                     /locus_tag="ACHBRQ_000850"
     CDS             complement(2737..3021)
                     /gene="groES"
                     /locus_tag="ACHBRQ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9592552.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3291..3686)
                     /locus_tag="ACHBRQ_000851"
     CDS             complement(3291..3686)
                     /locus_tag="ACHBRQ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9592553.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3763..4524)
                     /locus_tag="ACHBRQ_000852"
     CDS             complement(3763..4524)
                     /locus_tag="ACHBRQ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9592554.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTHCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4557..5183)
                     /gene="ytpR"
                     /locus_tag="ACHBRQ_000853"
     CDS             complement(4557..5183)
                     /gene="ytpR"
                     /locus_tag="ACHBRQ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9592555.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVACLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPVKHWTA"
     gene            complement(5199..5516)
                     /locus_tag="ACHBRQ_000854"
     CDS             complement(5199..5516)
                     /locus_tag="ACHBRQ_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9592556.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5513..5812)
                     /locus_tag="ACHBRQ_000855"
     CDS             complement(5513..5812)
                     /locus_tag="ACHBRQ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HFM9592557.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            5906..6052
                     /locus_tag="ACHBRQ_000856"
     CDS             5906..6052
                     /locus_tag="ACHBRQ_000856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592558.1"
                     /translation="MKIKDQTRKLAAGCSKHGFEVVDKATLTWFEEIFKEYYSYCQAP
                     KLPT"
     gene            complement(6109..6300)
                     /locus_tag="ACHBRQ_000857"
     CDS             complement(6109..6300)
                     /locus_tag="ACHBRQ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HFM9592559.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6461..7135)
                     /locus_tag="ACHBRQ_000858"
     CDS             complement(6461..7135)
                     /locus_tag="ACHBRQ_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592560.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKIVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(7141..7587)
                     /locus_tag="ACHBRQ_000859"
     CDS             complement(7141..7587)
                     /locus_tag="ACHBRQ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFM9592561.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7701..8204)
                     /locus_tag="ACHBRQ_000860"
     CDS             complement(7701..8204)
                     /locus_tag="ACHBRQ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9592562.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8201..8563)
                     /locus_tag="ACHBRQ_000861"
     CDS             complement(8201..8563)
                     /locus_tag="ACHBRQ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9592563.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8629..8778)
                     /locus_tag="ACHBRQ_000862"
     CDS             complement(8629..8778)
                     /locus_tag="ACHBRQ_000862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592564.1"
                     /translation="MSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNSQFTE
                     DEAEE"
     gene            complement(8806..10530)
                     /locus_tag="ACHBRQ_000863"
                     /pseudo
     CDS             complement(8806..10530)
                     /locus_tag="ACHBRQ_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10505..10976)
                     /locus_tag="ACHBRQ_000864"
                     /pseudo
     CDS             complement(10505..10976)
                     /locus_tag="ACHBRQ_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            complement(11100..11816)
                     /locus_tag="ACHBRQ_000865"
     CDS             complement(11100..11816)
                     /locus_tag="ACHBRQ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9592565.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12697..14112)
                     /gene="ply"
                     /locus_tag="ACHBRQ_000866"
     CDS             complement(12697..14112)
                     /gene="ply"
                     /locus_tag="ACHBRQ_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HFM9592566.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14123..14533)
                     /locus_tag="ACHBRQ_000867"
     CDS             complement(14123..14533)
                     /locus_tag="ACHBRQ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592567.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTGIPTSYLARRRRN
                     VSEELYEEILDYLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(14526..15134)
                     /locus_tag="ACHBRQ_000868"
     CDS             complement(14526..15134)
                     /locus_tag="ACHBRQ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592568.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15147..15605)
                     /locus_tag="ACHBRQ_000869"
     CDS             complement(15147..15605)
                     /locus_tag="ACHBRQ_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFM9592569.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15730..16126
                     /locus_tag="ACHBRQ_000870"
                     /pseudo
     CDS             15730..16126
                     /locus_tag="ACHBRQ_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(16867..17127)
                     /locus_tag="ACHBRQ_000871"
     CDS             complement(16867..17127)
                     /locus_tag="ACHBRQ_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592570.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(17186..17737)
                     /locus_tag="ACHBRQ_000872"
     CDS             complement(17186..17737)
                     /locus_tag="ACHBRQ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592571.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17727..17924)
                     /locus_tag="ACHBRQ_000873"
     CDS             complement(17727..17924)
                     /locus_tag="ACHBRQ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592572.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(17928..18512)
                     /locus_tag="ACHBRQ_000874"
     CDS             complement(17928..18512)
                     /locus_tag="ACHBRQ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592573.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(18920..19345)
                     /locus_tag="ACHBRQ_000875"
     CDS             complement(18920..19345)
                     /locus_tag="ACHBRQ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592574.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(19473..19865)
                     /locus_tag="ACHBRQ_000876"
                     /pseudo
     CDS             complement(19473..19865)
                     /locus_tag="ACHBRQ_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(19878..20084)
                     /locus_tag="ACHBRQ_000877"
     CDS             complement(19878..20084)
                     /locus_tag="ACHBRQ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592575.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISRVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(20071..20232)
                     /locus_tag="ACHBRQ_000878"
     CDS             complement(20071..20232)
                     /locus_tag="ACHBRQ_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9592576.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(20604..21560)
                     /gene="lytA"
                     /locus_tag="ACHBRQ_000879"
     CDS             complement(20604..21560)
                     /gene="lytA"
                     /locus_tag="ACHBRQ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HFM9592577.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(21936..23306)
                     /locus_tag="ACHBRQ_000880"
     CDS             complement(21936..23306)
                     /locus_tag="ACHBRQ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9592578.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(23613..24779)
                     /gene="recA"
                     /locus_tag="ACHBRQ_000881"
     CDS             complement(23613..24779)
                     /gene="recA"
                     /locus_tag="ACHBRQ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9592579.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(24834..26090)
                     /locus_tag="ACHBRQ_000882"
     CDS             complement(24834..26090)
                     /locus_tag="ACHBRQ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9592580.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(26175..27191)
                     /locus_tag="ACHBRQ_000883"
     CDS             complement(26175..27191)
                     /locus_tag="ACHBRQ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9592581.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(27199..27717)
                     /locus_tag="ACHBRQ_000884"
     CDS             complement(27199..27717)
                     /locus_tag="ACHBRQ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9592582.1"
                     /translation="MEYELLIREAEPKDAAELVTFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            complement(27707..28150)
                     /gene="tsaE"
                     /locus_tag="ACHBRQ_000885"
     CDS             complement(27707..28150)
                     /gene="tsaE"
                     /locus_tag="ACHBRQ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9592583.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(28236..28856)
                     /locus_tag="ACHBRQ_000886"
     CDS             complement(28236..28856)
                     /locus_tag="ACHBRQ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592584.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
BASE COUNT         8755 a         6551 c         5027 g         8876 t
ORIGIN      
        1 cgtcgattca agtgttatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag
       61 aagaatcacc aaatccctcg tattatcaac caaaaaattg cgcaaaagtt aattgaaaag
      121 atttctatga ccgatattgc ccatcagctt tccatctcaa cttcaactgt tattcgtaag
      181 ctcaatgatt ttcactttaa acatgatttt tcttgtcttc ctgagattat gtcttgggat
      241 gagtatgctt ttacaaaggg aaagatgagc ttcattgcgc aagattttaa caatctcaat
      301 atcatcactg ttcttgaggg aagaacacaa gctgtcatcc gagatcactt tcttaaatat
      361 gatagagccg tccgatgtcg cgtcaaaatt attactatgg atatgtttag cccttactat
      421 ggcttggcta aacagcttcg ctttcacatt gtacaacatc ttagccgtgc tatgagtcgt
      481 gtgcgtgtcc aaatcatgaa tcagtttcat cgaaaatccc atgaatacaa ggctatcaag
      541 cgctactgga aactcattca acaggatagc cgtaaactga gtgataagca attttatcgt
      601 cctacttttc gtatgcattt aaccaataaa gagattttaa acaagctttt gagctattcg
      661 gaagacttga aacatcacta tcagctctat cagctcttgc tttttcactt ccagaataag
      721 gaaccagaga aatttttcgg acttattgag gacaatctta agcaggttca tcctattttt
      781 cagactgtct ttaaaacctt cctcaaagat aaagaaaaga ttgtcaacgc ccttcaacta
      841 cactattcta atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat
      901 gcctttggtt ttcgaaactt tgaaaacttc aaaaaacgga ttttcatcgc tctgaatatc
      961 aaaaaagaaa ggacaaaatt tgtcctttct cgagcttagc ttttcttcaa cccactacag
     1021 ttgacaaaga gcctataaaa agaagggtta gtcattccct ccttttgtgt tttttataag
     1081 ttgttttcta tagaaagctt acatcatccc gcccatcata cttggatcca ttgctggagc
     1141 tggggctact ggttctggtt tattggctac gactgcttct gttgtcaaaa tcaagctggc
     1201 tacagatgct gcattttgta gggctgaacg actcacttta actggatcaa tgataccttg
     1261 atcaatcatg ttaacccact cgccagttgc tgcgttgaat cctataccaa gctcagcatt
     1321 tttcaaacga tcgataacga tagatccttc aaatcctgca ttgtgagcaa tttgacgaac
     1381 gggttcttcc aaagcacgga gaacaatatt acgtcctgtt gcttcatctc ctgtcaattc
     1441 caaggtagca acagctggaa tcacattggc aagagctgtt ccaccacctg caacaatacc
     1501 ttcttcaaca gctgcacgag tagcgttgag ggcatcttca atgcggagtt tcatttcttt
     1561 caactcagtt tcagttgcgg ctccgacctt aataaccgct acaccacctg acaatttggc
     1621 caagcgttct tgcaattttt cacggtcaaa ttcagaagtt gtagtttcga tttgagactt
     1681 gataaccgca acacggttag aaatcgcttc aggatttcct gcaccttcta caataaccgt
     1741 gctatctttg tccacggtca ctctcgctgc ttgaccaaga gcttcaattg tcgcatcttt
     1801 caactcaaga ccaaggtctt ctgtgataac tgttccgcct gttaagatgg cgatatcttc
     1861 aagcatggct ttgcgacggt caccaaaacc aggtgccttg actgctacta cgttgaaggt
     1921 tccacgaatc ttgttcaaaa caagagttgg aagagcctcg ccatccacat catccgcaat
     1981 aatcaagagt ggacgattgc tttggagaat gctttccaaa agtggcaaga tttcttggat
     2041 attggaaatt ttcttgtctg taatcaaaat gtacggattt tcaaggtcag ccaccatttt
     2101 ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg tcaaactgca ttccttctac
     2161 gacttcaagc tctgtttcca taccacgtga ctcttcgatg gtgatgacac cgtctttgcc
     2221 aactttttcc attgcttcag agatgtactc accaactttt tcagaacgag aagatacggc
     2281 tgcaacttga gcgatagctt ctttattggc aacagggatg gcgttgtttt tcaaagcttc
     2341 aactgctgcg gcaactgctg tttcaatccc acgacgaata ccgattggat ttgcacctgc
     2401 tgtgacgttt ttgattcctt cacggacgat agcttgggtc aagactgttg cagtcgtagt
     2461 cccgtcacct gcgatatcat tggttttaga agctacttct gatactaact tagcacccat
     2521 attttcaaaa tggtcttcca attcgatttc tttggcaatg gtcacaccgt cattggtaat
     2581 caagggtgaa ccgaatgact tttcaagaac gacattgcga ccttttggtc ccaaggttac
     2641 tttaacagtg tctgcaagaa tatcgacacc acgaaccatg gctgaacggg catctgatga
     2701 aaatttaatt tcttttgaca tacttacttt ctccttctat tcctcaatga ttgccaaaat
     2761 gttagcttcg cctacgataa tgtacttttc atcgccatct ttgacatcaa gacctgcgtg
     2821 ggcttcaact aagacacgat ctccagtttt aacacttgga gcaaccaagt caccgttcaa
     2881 ggtacgaaca ccttgtccag tagccacaac ttgagctgtt ttggtttttt cttgggctga
     2941 gcctgcaagg acaaagcccc caacggtttg ttctttttct tctattttta agaccacacg
     3001 gtcccctaat ggtttcaaca tctgatttcc tccataatga gatagatatt attagcactc
     3061 tttgaactgg agtgctaatt cataattcta ttgtatcact tggtcagaaa tagtcaagaa
     3121 aaaagtctga ctttctcaag ataaaaagcc tgagaccaac tcagactttt taatgtttat
     3181 actcaatgaa aatcaaagag caaactagga agctagccgc aggctgctca aagcattgct
     3241 ttgaggttgt ggataaagct aacgtggttt gaagagattt tcgaagagta ttaaaatggc
     3301 aattcttcct cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcatg
     3361 gcacgttgag cacgactttc taagagttgg aatcctgtca caaggacttc ggttacatag
     3421 ttcatctgac catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga
     3481 ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg
     3541 acaaaatcag cttcacgttc accgttttgg tctttgtaac gacggttgac agcgatagtt
     3601 gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgc
     3661 ccaatcatga taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa
     3721 tcaaaaaagt tacagaaatt tgtaactttt cgaggaaatt tcttattttt tatgaatcat
     3781 gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa cacggcgagg
     3841 ctgactggtc acatagacta ctgtgtctgc aatatcctga gcttgcaagg cttctattcc
     3901 ttggtaaacg gacgcagccc gctctttatc accatgaaaa cgaacagttg agaaatctgt
     3961 ttcgacaatc ccaggctgaa tggttgtcac cttgatatcc gttgcgatgg tatcaattcg
     4021 cagtccatcc gaaaaggtct taaccgctgc cttggtagct gagtaaacag cggcaccagc
     4081 ataggcgtag attcctgcgg ttgaccccat attgataata tgaccttgat tgacttttat
     4141 cattgctggc aagaaacagt gagtaaccgc catcagacct ttaacattgg tatccaacat
     4201 ggtcaacata tccaactcct catagtcttg atagggagcc aagtcaagag ccagtccggc
     4261 gttgttgacc aagatatcaa tcctccctat cgtttctaga atatcagagc aaacagtctt
     4321 caccatgacc atatccgtca catctagtgg aaaagtccag acggtttgat ttggaaagct
     4381 tactgcaaac tccgacttga ggatttctaa tctgtctatc cgtcgtcctg ttagaacgac
     4441 atcctcaccc tgctccagat aagcacgcgc aatcgcttca ccgattcctg aggttgctcc
     4501 cgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac aagttcctag
     4561 gcagtccagt gtttcactgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     4621 agcaccccac gtttttgcgg agcatttggc aaatgcaatt cacgaggact acacatcatc
     4681 ccaaaactct tttcaccacg aagttcgcct gggaaaatga aattcccttt tggcatcatg
     4741 gcaccaggaa gagccacaat ggttttcaac ccaacacgcg cattaggtgc tcctgcaacg
     4801 atttgcacta tcttgtcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     4861 ggatgggcta ccatctcaac aatttcacca acaacaaact taggttcctt gtcattgaca
     4921 atttcttctg taaaaccttc cgcctgtaat tcttggttca aacaagcgac ttgctcatct
     4981 gataaaaaga cttgacctcg ctctgcaatt tcaaacaagc tcgaaacctc aaaaatgttc
     5041 caagctactg tttccccatt ttctttgagg aaaacacggg ctaccttgcc tttgcgttcc
     5101 acattcagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     5161 tctttgttat atgtaaaaat cattgtttac tttttctcct atttcaatcc tgctaaaaaa
     5221 tcgttaattt gctccttact tttacggtcg cgattgacaa aacggccgat ttccttgtcc
     5281 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     5341 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc aatctctggt
     5401 aaggcaggat agatataacg gcaatcgcca caccagtccg ccacaaaaag gaagaccttc
     5461 ttaccgtctt tttctactaa agatgccaat tcttctaaac ttgctggttg tatcataaga
     5521 cttcctcctc atagactagg tcttcatttt catagacaaa agtataatga cggccatcct
     5581 caaaaatgac gccaccaagc aagctctcta gactgctttc atagacttga acatagaggg
     5641 tcgcaatttc ccccatgtct gaaaaatggt ctcgtacaat cgctgtcaac tcttcctgag
     5701 tcttcatgag cttatggtca tctgcaactt ttttcgtagc aagggcaagg ctcccaatac
     5761 caagcagagc cagacctgcc atccacattt ttttagcttt cataccattc attttaacac
     5821 aaaaaaggct ttaggacaaa tgaggaaaca gcaaaaaagc aagtaaaaag cctcttcctt
     5881 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagaa agctagccgc
     5941 aggctgctca aaacacggtt ttgaggttgt agataaggcg acgctgacgt ggtttgaaga
     6001 gattttcaaa gagtattatt cttattgcca ggcacctaag ttgccaacgt agtaaccatc
     6061 aggtgtgtag gtattgcgaa gcatcttacc tgatgaagcc agataatact acttgccatt
     6121 gtctttgacc caatcattcg caatcatgga accagaagaa cttacataat accattctcc
     6181 cttgtcataa acccaagtac tgactttcat ggttcctgag caattaaagg caaaaaaact
     6241 gtccaatatc attcgttttt taaaagcatt tgacactaca tcatctacgc tgtagtacat
     6301 agatacatca aacgtaaaag ccaaaatatc gaaaaatata caacttttaa aggcagcatt
     6361 tcatttttta aagtctaaca accatagaac tgaaatcaga gcaaggcatc aatttcctac
     6421 tcttagtacg tctattttga tggttgaagt gaagattttt ttatttaacg tagacaattt
     6481 ccaacttccc aaaagccaca tctccacgga tagtcaaggt tttgctggtt ggggctacag
     6541 gagcgtcagc cttagctgca ccaaaggagg tttctacttt caaatctaca cgccagtgtt
     6601 gtggaacata gacggttgca tttccgaagg ccacttcaat atttaaagtt gcaaaatcac
     6661 ctagcatctc tgcattatca tagtagattt tagcatcccc aaaagcgact tccacttggt
     6721 catctacgag atcttgatct tgcttataga aggtcccgct accaaaagcg acttccttat
     6781 ccgtgacgac tatttttttc ccattgtacc accatttttt ttcattccag aacttacttg
     6841 aatgcgtcag ataaccaaca ccaagtacca ccaagatgct agcccaaata agagaatgat
     6901 tggtaactgg taacaagtca taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa
     6961 aaactgccga agtgagatga cgtctaagga tggactcaat ggacttataa gcaaaaaaaa
     7021 caatacctag taaaggccat attttaccat ccaaagaagg aattccaaaa ttcccttgca
     7081 gcaagatcca agctgctaaa accaataaaa caataccaaa tgctttcttt ttcatgtttt
     7141 ttacctcatg tttcttagat tttcttttag gagggattgg taatgccgtg agacatgaac
     7201 ctccttgtgc gtctgataaa aggaaatggt gcccgttcct gaaaaggact tgtccactga
     7261 gtaaatctga cggatgtttg cgatcgttga cttggaaact cgattaaaat agcgaggcaa
     7321 gatagactcc aactcataga gcttgaggcg aacctcataa gcttccttct ggttatgagc
     7381 gtagatcttg ctcccttctg tttcaaagaa gagaatttct gacaggtcta gataatattc
     7441 acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac tcttgcaaga gccgttgcaa
     7501 gtctgcaatt tcatctgtca aagtgggggt cttgatgaca atttcagtct cctctaaatt
     7561 gccgtcaatc tcaattcgta acttcatcac atctcctttc tgactctact atatcaaagc
     7621 taagttagga atacaagggc aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta
     7681 ggactagctt aacttacatg tcagaatagg aagaaaagga atccccacac caaaccacaa
     7741 gcatagccaa ccaagacatc cttgggataa tgaacacccc ctaaaaccct caccaacccc
     7801 agcaaagctg aaaagatcaa gcatactaag ccaataggta gactagcatg cagacaggcc
     7861 atggagatga tagttgccga aaagacatgg cgactgggca tcgaccgtcc cgaactatcc
     7921 ttatcaagaa ggggacagat atcccaagtt tcataaggtc tcgggtgatt gattttctta
     7981 cgaaacaggg acaaggtcac aaaacctgat gcaggaataa gcacataaac cccaacttgc
     8041 tgaccaagtc ctagttgcag gtaagtggtg actagcaaga tcagatagac caggggcatg
     8101 gcgactgtca tcaaacgatt gaaactgcgt aataggaata aaagaattgg acgactagtt
     8161 agtctggagc tgatatttcg ataccattct tgataatttt tcatttatgc ttctcatact
     8221 tttctagaac ttgttgagat gttttcccct tggcaagcca atccactagc ttgtccaagc
     8281 gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat acggacaccg caaatcttcc
     8341 ctgtaattgc aattcgttca gggacataat atggtgcttg tctaaaaaaa tctccgtaag
     8401 taacatccct atccagacaa gcaagaacat cactcacttc ataccctgtc aaccaactgg
     8461 tcacttggtg aactgactct gcctttccac cctttcgctc aacttttgca atcaaggcta
     8521 agtagacagc agcaaatttc atattgtata atttctgact catacttctc tctttctatg
     8581 tgtactagta taaataagaa aaagaaggcc gtcaagcctt cttttgattt attcttctgc
     8641 ttcatcttct gtaaattgac tattgtacaa gtcagcgtag aagccacctt gcgccatcag
     8701 ttcctcatag ttgccttgct cgatgatatt tccatctttc atgaccaaga tcaagtctgc
     8761 atttcggatg gttgacaagc ggtgggcaat gacaaaggat gtgcgtcatt ccatcaaacg
     8821 gtccatggct ttttggatca attcctctgt ccgtgtgtca acagaagaag tcgcctcatc
     8881 caaaatcaaa agcggtgcat ccttaagaag ggcacgagca atagtcaata gttgtttttg
     8941 tcctacagac aaggtcacgg tgtcatccaa gatggtatca tagccatctg gcaaggtcat
     9001 aataaagtgg tgaattccca cagccttact agcttccatc attcgttcat cactaatccc
     9061 tatttgatta tagatgagat tgtctcgaat agttccttca aagagccagg tatcctgcaa
     9121 gaccattgaa aaggcatcat gcacttctga acgcgtcata gccttggtat ccacaccatc
     9181 aatgtgaata cttcccttat caatctcata gaatttcatc aaaagattga caatggttgt
     9241 cttaccagtc ccagtcggcc caacaatggc aaccttttga ccagcatgag ctgtcgcaga
     9301 gaagtcatag tcttgaacat tgacaccgtc caccagaatt tctcctgctg acacgtcgta
     9361 gaaacgtgga atcagattga ccagagttga tttaccagaa cctgttgacc caataaaggc
     9421 cactgtttga ccagtttctg ctttaaagct aacatgttca ataactgcct ccgaatttgc
     9481 cgcatagcgg aaggtcacat ccttaaactc gacctgacct ttgaagtttt catcagtcag
     9541 ctgcacttga acagggtttt ggatagaaga atgcaaatct aaaacttgat taatccgctt
     9601 agcagagacc atagttcggg gaagaacgat gaagagtgct cccatgagaa ggaagcccat
     9661 gacaacctac atggcataag acatgaaaac aatcatgtca ctaaagagag gcagacgcgc
     9721 tatcgaagca gcgtcgttaa tcacataggc cccaatccag taaatcgcta cactcaaacc
     9781 gcttgaaatc cccatcatga taggattcaa aatagccata agacggttga caaacaaatt
     9841 caaacgggtc aattcatcat ttactgctgc aaatttttca ttttgataat cctctgcatt
     9901 gtaggcacga acgacacgaa tacctgttaa actctcacga gtgatactgt tcagtttatc
     9961 tgtcaacccc tgaatcaagg actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat
    10021 caggacgttg acaatcacta ccacaagtac ggcccagagt cagtattctg aatgacctaa
    10081 aatcttccca atagcccaga tagccataat tggaccacgc gttaccactt gcaagcccat
    10141 agtaatcaac atttgaactt gagtaatgtc attggtagta cgcgtcaaga ggctaggaat
    10201 tgaaaatttc ttaatctctg tctgcgagta atccaaaact cggttaaaaa tatcacttct
    10261 cagcctacta gtataagaag ccgccactcg ggatgcaaaa aatccaactg caactacgga
    10321 caagaaggca agaaagagca ttcccatcat catgcttgcc ggctgccaca actcatctaa
    10381 attagtttct tgactaccta gcaaatccgt aattttcgag atataggtcg gcacttccaa
    10441 ctctagatag accgaaaagc aagtaaagag aatggctagt aaaatcatcc cccattcttt
    10501 tctactaatt cttttggtta atttctttat tctctcctcc tattcccttg atattttgcc
    10561 tgtagttgac cgagaacttt ctcaaaaatc agtaattcat cttcatcaat gtcttccatc
    10621 aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg
    10681 tccgtcagac gaacaaactt agcccgctta tcaacaggac tcgcctccaa ttccaccaaa
    10741 ccattttgca ctatacgctt aaccagatta ctagcaacag acttggtaat attgagttcc
    10801 tgctcgatat ctttaatcaa gaccaagtct tggtttttct agcgattatc caaaaaacgc
    10861 acaacctgac cttgcggccc acccataaat tcaatgccgc aacgtttggc ttccttttgc
    10921 accatcaggt gaatttgatg accaaaacgc ttaaagacta acatcggttt atccataatc
    10981 tcccccttct aaataaaaat agttctctgg agaataatta aatttctatg agaactattt
    11041 tcttgattaa aaaaatccca agtgattttc tcacttagga tcatgttcta taggttaaat
    11101 taaaacccat ctacgttcgt ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg
    11161 taaagttttt caaaggtttc aaggtcttcg cctgacaatt ccacttctga ctgaggaatc
    11221 atttccaatt cagtcacttg gaattcttca ataccagact cacggagagc aacgatagcc
    11281 ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca
    11341 tccacatcca catccgcttc gagcaattgc tcaaagactg cgtctgcatc ttcacctcca
    11401 aatacaataa cacctttgtt gtcaaagagg taagaaacag aacctgaagc gcccatgttt
    11461 ccgccgtttt taccaaaggc tgcacggaca ttggctgctg tacggttgac gttagaagtc
    11521 aaagtatcca caattagcat agagccattt ggcccaaaac cttcgtaacg tccttctgta
    11581 aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt tatcgataat gtgttttggc
    11641 acttgggctt gtttagcacg gtcgataacg aatttcaaag ctgagtttga ttctggatct
    11701 ggatcacctt ttttagctgc tacatagatt tctacaccaa attttgcata taccttagag
    11761 ttagctccat ctttagccgt tttcttggct acgatattgg cccatttacg tcccattagg
    11821 aatctccttt tttcacattt taatctttat tattataaca caagtttttt tgattttcac
    11881 tagaggaaat ggattttatt agcaaatcaa gctaggatag cactttacct gctaagatgg
    11941 tcttaccttt ctatctttat caacagacac tcatccacat tcaaaaaaca aactagacca
    12001 ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt cagctcaaat tactgtttga
    12061 agtttgtaga tataagcgac aaaaacaatc atactgcacc ttttgttgac agtctactcc
    12121 agacatatca tagttcaagt aaatactttg aaattcaaca gttcttatag gcgctattgt
    12181 attctaagaa atcaatagaa gagtttctaa gcaaacctct aatactcaat aaaaatcaaa
    12241 gagcaaacca gaaagctagc cgcaggttgc tcaaaacact gttttgaggt tgcggatggg
    12301 gctgacatgg tttgaagaga ttttcgaaga gtataattta cgtgttccca agatggagaa
    12361 gttagactag tacactggca cttctaaacc attgctagca attgatttgt tcatatttaa
    12421 tttcattttt tccataaatg ggtattagat ataaacagca aaatatttcc gatacgtgtc
    12481 gttcttgaat ttccaatcat ctaaaacaag taaaggataa tcaatcccct gtatatcaag
    12541 gaattggcta ccctttttac ttttttacac attctgtttg atagattcat tttaacatca
    12601 cgagcatact ccaatggaaa tcgctaggca agagataaac tttcagatat ccgcagagag
    12661 atcatcgcct ctttttgtcg caagcattct cctctcctag tcattttcta ccttatcttc
    12721 tacctgagga tagagagttg ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa
    12781 atcggttttt tcataaaccg tacgccacca ttcccaggca agcccggtac actctctaat
    12841 tttgacagag agattacgaa cattcccttt taaaggaata ctagtggtaa agtgagccgt
    12901 taaatcctgc ccatttctgt cccaagcctt aggagtcaag acttccttac cttgatgatc
    12961 ataggataat tcattccaag taatataata ttgggcaaca taggcaccac tatgatccag
    13021 cagtaaatct ccgtttctgt aagctgtaac cttagtctca acatagtctg tactattttg
    13081 aaaggtcgca actacattgt cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg
    13141 atgatctgct gtaaagcgac tgccttcttg aatcaagtcc tctaccatat ccaccttgcc
    13201 tgttacaact cgggcacccg aacttgggtc gccccctaaa ataaccgcct tcacttctgt
    13261 attgtccaaa atctgtttcc actctgtctg aggagctacc ttgactcctt ttatcaaagc
    13321 ttcaaaagca gcctctactt catcactctt actcgtggtt tccaacttga gatagacttg
    13381 gcgcccataa gcaacactcg aaatatagac caaaggacgc tctgcagaaa ttcctctctg
    13441 ttttaaatcc tctaccgtta cagtatcttg aaacacatct cctggatttt taacagcatc
    13501 tacgctgact gtataataaa tctgcttaaa attaacaatc tgaatctgct tttcgcccga
    13561 atggacagag ttaaaatcaa tatcaagaga attccctgtc ttttcaaagt cagaaccaaa
    13621 cttgaccttg agttgttcca tgctgtgagc cgtgattttt tcatactgca ttctagctgg
    13681 gacattattg acctgaccat aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc
    13741 gcgaacactt gaattgctgg ggtcttccac ttggagaaag ctatcgctac ttgccaaacc
    13801 aggcaaatca atactataag tcatcggagc acgatcgacc gcaagaagag tgggattatt
    13861 ctctaacaag gtctcatcca ctacgagaag tgctccagga tagaggcgac tgtcgttggt
    13921 agctgttaca gaaatatcac ttgtatttgt cgacaagctc cgcttctttc tttcgataac
    13981 aacaaactca tcgggtagct gattaccctc tttgatgaaa cgattttcaa tactttctcc
    14041 ctgatgggtc aagagtttct ttttatcgta attcatagct agtataaagt catttactgc
    14101 tttatttgcc atcttctacc tcctaataag ttcctggatt gagttgcata aactcagact
    14161 tgttcagcga aatcagccgt ggttggacta agtaatccaa aatttcctcg tacaattctt
    14221 ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg aatgcccgta ttatccaaca
    14281 taaatacctt atctaagtca atcaaggttg gtcttgtaaa aggattacga gctagatccg
    14341 gctcttctat cataaagttc ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct
    14401 gatttttaac caactctttg tttttagtca tgctgttgtc aatacagata tacatatgat
    14461 tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa ataaagagct acaacatctc
    14521 ctctcttaat caagcaagag cacccccttt tctcctaatg taacatagac aggattgacc
    14581 aagtcttctg attgactcag aatttccaaa gtttgagttt ggcgcgctgt caatttagta
    14641 gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa ccttgacaat ataatccttc
    14701 tccaaagctg actggtaaat ccacatcaga tgttgtctgt cctgagaact caagagagaa
    14761 ggattttcaa gcctcccgat agtctgataa aaatcaaaaa caggagctaa ctcctgccaa
    14821 tctgattggc tagttgtcaa ggctagaaaa agggctttgc gagctgatac ttcttggtta
    14881 gccttgagag ttactttccc ctccaagttt tttagaaatc gggaaactcc agaaagcaaa
    14941 tttttctcta actgcgagaa ataaaaacct ttcgttccca gacataagtc tttcatgtcg
    15001 ctttctctag caaataagag ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg
    15061 gtcgcgctca tcttttcctt atcggcttct ttttttaacc agagcaaggg cgacaggtag
    15121 ctggattgag acatttcctc tacctcctac tcttttttaa ctggagcatc tgcactagct
    15181 gccacttctt ttgactggat actttcccac tggttaatct cctctgagat aagaccttcg
    15241 catgtcttga caaatagggc aaaagccttg gtctttcctg catatttctc cgtttggcat
    15301 tgatagagga atttttcttt ctccaggagt tgcgcagttt tttggtaaga aatccaattt
    15361 tcctttgcat tatacaaatt gataatcccc tcacacagca agccgagact ggataaggca
    15421 accgaaatca aacggtagcg atcacctggc ataggaatag cacaaaagac agctatgagg
    15481 aaacctgcca cgatttctgt tatttttaat accttatagc gtctacgatg ttgaacgctt
    15541 ttctttaaaa aatgagctat ctgtacgtct aatcgctctg tcaggtacat ttcttctggc
    15601 gtcatattcg taactccttt catttacttt gataaccata gtataacata tttgaatatc
    15661 aaagtatagt aaaaactgga ctataactta ttagacttcc tgcgaaacaa aatatggtat
    15721 agtagttcta tgaattatga agcaagtaaa caactaactg atgcacgatt taagcgtctt
    15781 gttagtgttc agcgtaccac ttttgaagag atgttagctg tattaaaaac agcttatcaa
    15841 aaaagtcgga caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat
    15901 atatcctcaa gcacaaactc cacgtaaatc cagcaaaccc aagccgctaa tagctgaaga
    15961 taaagcctat aaccatgcgc tatctaagga gagaagcaag gttgagaaca tctttgccaa
    16021 agtagaaatg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
    16081 acgaatgaat ttgattgctg gtattatcaa tcatgaacta ggattttggt ttcgcaggaa
    16141 gtctattata tatgcacaga catatgaaat ctttttgaga cagcagttat gatatattta
    16201 tccattcaca acatcttctg tactaatact acaatattgg tacattttga ttacacaatc
    16261 gtttcaaaaa atatgatgga agcctgagta ttttatagta attcccttcc tatcaactag
    16321 atcaaccagt tgagtgttga atgttgaaac accctagcct cagcaaagga ctatgatcag
    16381 catttcctag ccaataggac gctccaaatc acctattcta agtccatcac tagcttggtc
    16441 agtaagatac agactgcaag gcgagactga cgtggttgat gagattttgg aagagtatta
    16501 caatcataga ttcaccagta gacttgaccg cagatttaac tatagtagat tgaaactaga
    16561 atagtacatc tctgcttcta aaacattgtt agaaatcgat ttgactgtcc tgatcaattt
    16621 gtcctgttct cgtttcattt cactatacta aaaaaccacc tgaaacaaat caagtgacta
    16681 gaggataaaa acaaagacta ggcaaaaagt tttgaaaagc aaaaaacgca taatatcagg
    16741 tcttgaaaaa ccttgatact atacgtttga ttgtgggaag attgacttca ttttctcctg
    16801 aaattgaagt tttttctagt ttccagatat agacgaattc aatatagcca tctcgctaca
    16861 attattttaa ccatcgagaa aatttttcag aatttcaaaa atttcatttt gatccaatga
    16921 aatgtcatca ttttgaagaa tagttgactt cacaggctct aattctatca caaagcggtg
    16981 tatgtctgct tttaccgcac tacttgatgt tatagccgat aacttttcat ctcctagcaa
    17041 ggaagctgtc aaacgggtaa tgtcattcaa atgcttttta atggacttac ttaaaccttg
    17101 agcacctgta gcagagcgtt ccctcatatc cagccaagct ttagccttaa aaataattaa
    17161 tactcaatga aaatcaaaga gcaaactagg aagctagctg caggctgctc aaaacactgt
    17221 tttgaggttg tagatggaag ctgacgtggt ttgaagagat tttcgaagag tataaaccac
    17281 aattactcaa taccgaatac ccctgaatgg tttctttttc atgcaccaat atattataat
    17341 aatcttcatc caataataag gctgataaac tagcatcttg gtcaaaatgt aagggaattt
    17401 ctcgaccgtc cttctttaat ggatattctg gtaagatact aaatagttca atcatagaag
    17461 gaaactcagg attagttgtt gtaaatcgaa aaagctgcgc tttctcatct ttctgacttc
    17521 cttgatactc tcccaattct aaaaaatgat tcaaggtagt ataaaattcc ttatttttta
    17581 cttcataaat gatgaccata tcataatctt ttgttgtgcg acttttaaat ccttgcgaat
    17641 ccaatacgat agaggtagca gttcccccaa tcagaacata atagttctga aaatccgcaa
    17701 acatttcttg aaaaataact ttcgtattag ctggcatcat cttctcccag atactgtaat
    17761 atcatatttt ctagtgcttc actctcttcc tctatacgtg ggtcatcatc atcttttaag
    17821 gtcaaataaa gagaaatcgg atctacaaat tgtttatcat gattattttt aaaatcattc
    17881 caaaactcag atacaaaagg acgatatttc catatctcta gcatctttca ttttaaaaca
    17941 tgctgagaaa gtggcaagga taactgattg aattttctct gccatatgac atagctagta
    18001 ttttcatccg tttcagctaa aaaagttgaa tgcgacaaag cataagcacc accatataga
    18061 aggttagaaa cagattttat ctgctttata tcgccatctg gcaataaaaa ggcaatccac
    18121 gttaattgtt cgctaggtgt taattcctta gggatttcag tatcattcgc attgagtact
    18181 agtcccaatg gagggaagaa gaggtttccc ttaaagtcta caaacggaac tctagcttga
    18241 agtaattgct ttttttcact gtctgataac ttcgaaaaca ccaaaacaac atccatattg
    18301 gctttttcac ccatagtgcg agcctgagta acaaatgaac tcaaactccc ccttctcttt
    18361 tctttaatca ataaaaatga ttccttatga aaagtcattt gagtatactc aaaccttttt
    18421 acaaaactga ttggtaaagt caaagtcagt tcctcaatac tgacatcaac ataaaaggat
    18481 tgaaaaattg acattatctt tttgacattc atgttttttc cttattctat catttttatt
    18541 atattttatc attcggtata ataaaatata agttttttga taaaataacc aaaaactact
    18601 cgctatcccc tctcacatta ccctacatat cgtttgacat gcgactgata gttcaggaaa
    18661 acttcaagga gaacttttct ctcatccact atgcaggact tactatttca cttctactcc
    18721 tataggctca atttgacact tttctttttc gaattgctca tatgcctcct gaatagtgag
    18781 ttcgatttat ccaaatttat ttttgtctat tttggtgaat acttccacga agatagtctg
    18841 aaaatgaatt tgcaccaatt ttccttctct ttttaagcat attattccag atatgattgc
    18901 cctcttgttc agtaataaat tacgctttaa acgcttcaat cagtatatct cctgttgtca
    18961 tatgttctaa agaaaattct tctacatatg gtttaacatc tcttaggtta ttacttccta
    19021 atatcccatt atgctttttc gctaagaaaa tagaagtcgc ttctccctta ccaataatct
    19081 tgttactatc ataatttctt gttaaatctc tatataatgc gtattcttca gttccaatgt
    19141 ctatgctcac aatctcagct gaacccttag ttaccaactg gtctatccta gattttaaat
    19201 ggggaattgt aggtatattg atttcatcat acacctcttg tggaataaca attttacccg
    19261 aatagagctt ttctaaaaga tgttcagtgc caacccataa aaatgctgaa atacaatctg
    19321 catcaataaa tacacgacta gtcaaacgac aactccccct cttcatctag cccatataca
    19381 atatcatatc tgaaagcatc tagtaacagt tcctcatact tcccttgcga aattctgtta
    19441 ttttctaaaa gttgttcagt taaattaata tattatccta ataccgtttc ctttttactt
    19501 tctgacaaag gacgatataa acttgtatca tagcctaatc ttgaagctgt ctctataaca
    19561 ctaatatcca tatttttaat ttcttctgca tcaaggtatc catcattcct caatctatat
    19621 aacatagctt tatgactgat accataaaac tgacccaatt ttataatatc ttctacttca
    19681 agatgagttc tattggcatt ttctctaatt tcctcaacca tcctatacag tgaagatggg
    19741 aaaattaaaa aataagaagc aaactgatcc gcttttcttt cagtttcatc tccttcacca
    19801 atcaagataa gactgactga actcttcttc acatcatcat aataaagatg atacagttca
    19861 tgtgctaaag aaaatcttta ccttcctaat gacatgtctg aattgactgc aatgagactg
    19921 aactgagttc ctttataaca gactctgcta atattctttc cgagtccata aaataccagc
    19981 gtcaaatttt ctatcttttg taccaattta aaaatatcta tcggcgattc accatcagct
    20041 cccaactttt ttctaagatt tggagcttta ttacttaaat ccattcgtga tattcctttc
    20101 acctttttcc attttggcaa tcatgctttg attcaaagac aaatactcag caatcttatc
    20161 ttgagtaaga tttgaagata ttctaagttc ttttattctt tttccaacat cacatacatt
    20221 tatcacatac atttatcaca tacatttatc atattcatac acctctcata aaaagaatag
    20281 cacactcttg tcataatttt ttaaataaaa aaattatgac aaaacaagga agcaatttat
    20341 tgatgctgct taaaaatcta aaattgatga gttcctatca aaaatcgtat cttcaacctc
    20401 aaaacagtac ttaaagctat ccgactcggt ttacattgtc aaatttagat tttatttgag
    20461 cataactttc tagtttgctt tctgattttt gtttaatata gtagcaaaca ttgaaggaaa
    20521 tatctccaca aaaaaacgca tactattaag ctttttcaag acctaataat atgcgctgtt
    20581 ctgatttgaa agacattcca ttattatttt actgtaatca agccatccgg ctctactgtg
    20641 aattctggct tgtctgccag tgttccgtct ggtttgaggt agtaccagcc tgttccgtcc
    20701 gctgactgga taaaggcatt tgataccatg gcgccttctt tagcgtctaa gtagtaccaa
    20761 gtgtccttgt acttgaccca gcctgtcttc atggcacctt cttcgttgaa atagtaccac
    20821 ttatcagcga ttttcttcca gcctgtagcc atttcgcctg agttgtcgaa ccagtaccag
    20881 ttgccgtctg tgtgcttcct ccagcggtct gcaagcatat agcctgaact gtcaaagtag
    20941 taccaagtgc cattgatttt ctcaaacttg tcttttggat aagagccgtc tgaatgtacg
    21001 taccagtagc cagtgtcatt cttctgccag cctgtttcaa tcgtcaagcc gttctcaata
    21061 tcatgcttaa actgctcacg gctaatgccc catttagcaa gatatggata agggtcaacg
    21121 tggtctgagt ggttgtttgg ttggttattc gtgcaatact cgtgcgtttt aattccagct
    21181 aaactccctg tatcaagcgt tttcggcaaa cctgcttcat ctgctagatt gcgtaagagt
    21241 tcgatataaa ggcggtagtc cgtcatgaac tcttctttgg ttgaatggct ttcaataagt
    21301 tcaaccgctg cataggtctc agcattccaa ccgcccccaa cgtcccaggc accattatca
    21361 acaggtccta cctgcatgat gcaaccgttc ccaacaatgt gcgagaaaaa acctaattct
    21421 gggtctttcc gccagtgata atccgcttca ttctgtacgg ttgaatgcgg attcccagtt
    21481 gagtgtgcgt gtacttgcct atatggttgc acgccgactt gaggcaaatc tgttcttaat
    21541 ttactcacat taatttccat attctactcc ttatcaatta aaacaactca ttttttacaa
    21601 tccagaacca gaaactcctt tatttctacc ttacaaagaa gacaatctta gtcacgatta
    21661 ggcttgtaga tagaacctca aaacgcacta ttttgacact gtaaatagga ctgacaaggt
    21721 ctgcattcta tctacaataa caccccagac taaaaagctt ttcaaaagta tatttttaca
    21781 gtctctatat gtccttttca taaatactat actttattat atcatataaa agaagtcaaa
    21841 agtctgttaa actattttca acaccaaact aaagaagaga acacaagttt tttcgatgtt
    21901 cactagagga aatggatttt attcagtaaa tccaactagg attgcacttt ggttgccaaa
    21961 attgcctttc cttcttttat caagggatga cggaatagtg agaagtacag ttgaactgtc
    22021 atggcaaccc agataagagg ttcacaaagg ataacaccct tatatcctgc ccaaggaata
    22081 atcaaaacca caaaaacgat ttttccgatt agttcaataa agctagaaac tagaggaagg
    22141 atcttctgcc ccaagccctg caagcaattg cgataaatca acaagaggct caaaatggga
    22201 taaaaggctg aactgatttg cagatagaga cttccatttt ctatcaagta accatctgtc
    22261 gaactagcca agaaggaaac caaggctgga ctggcaaaaa agaggaaaat acaaacaaaa
    22321 actgcccagg atatacttaa acgactgccg attcgaagac cttgaacaat gcggtcaggt
    22381 cgcttagctc ctagattctg agaagcaaag gtcgtcattg atgcagaaat agcggtcata
    22441 ggaagaaggg caaaggtcat aatgcgtcga gctgccgtct gggcactaat aatcactgca
    22501 ccaaatgtat taacagaaaa ctgtaaaatc acactaccga tagatacaat tgaactcatc
    22561 aagcccatag ccaaaccttg ctccaagaga tccgcgtaca agcttttgtc ccatttgaaa
    22621 tgtttaaact gtggcaagag ttctggcaca cttttacgaa tataataaaa gcagagaacc
    22681 gctgataaac cttgcgaaat aatggtagca agtcctgcgg attgaactcc cagatgcaat
    22741 tgcgtaataa aatagagatc cagaaccaca ttaaccaagg cagagaaaat cagaaatccc
    22801 agggctgcta gactgtcacc aatagaccgc aacaagcctg caaaaagatt ataagcaaag
    22861 ctgacaccta cacaggtcac aatcatagaa atatattgat aagattgagg aagaatttct
    22921 gcaggagtat ctaagtattg caagagagga tacaagccaa gaaagcccag caacataact
    22981 agaatgctca aaagagcacc taaaatccag gtggctgcta ctgcttcctt gattttagtg
    23041 aaattccgag ccccataata acgagcaatg acaatcccca tgccattgcc aacaccaagt
    23101 gtaaaaccta caatcaggtc aaaaatcgct gtcgtcgctc ctactgcagc caaggattct
    23161 tgaccaagaa atcgtccaac aatcaagaca tcagcagtgt tatagagctg ttgaaaaata
    23221 tttgatagca agattggaaa ggtgaagctt aagagcgagg gaagaatcgg accatgtatc
    23281 aggtccactg ttcgtttttt attcataaag ctattatatc agctttccag aggagcgaca
    23341 agaaacagca aacttatata tagcggaatg aaataaggac agtcaaatcg atttttaaca
    23401 atgttttaga agtagatgtg tactattcta gtttcgatct attatagttg gttgagtttg
    23461 gaatagtacg ctgtagctgc taaaacattt ctagaaatta atttgacttt cctaataaag
    23521 ttgttcatat cttatttcaa gctactatat ataatctaaa aacttgaatc aaaaatcgaa
    23581 aaagtagcgg atttctccac tgctttaaca gcttattctt caatttcgat ttcaagttca
    23641 tcgcctaagt caagcgtaac ttcttcattc actgcttctt ctttctttgg ctcatctttt
    23701 ttgttttcag tatcttgttc tgaaacttct tctccatcaa tcaagccaaa tttagaacgg
    23761 acttgcttat caatttcatc aaagatttct gggtgctctg ccaagtattt cttagcattc
    23821 tcagaacctt gcccaatttt ttcatctttg taagaatacc aagcccctgc ttttttgata
    23881 atatccaaat cgcttgcaat cttcaaaagc tcaccagtct tagaaattcc ttctccgtac
    23941 ataatttcaa ctacggcttc cttaaacggt ggagctacct tattttttac aaccttaatc
    24001 ttagtttctt taccgacatt ggtttctttt tgatcaccag ttcccttaat ttgtgtatta
    24061 ccacgaacat ccaagcggac tgaagcatag aatttcaaag cacgtccgcc cggtgttgtt
    24121 tctggatttc caaacatcac tccaactttt tcacgcaatt ggttgataaa aatggcaatt
    24181 gttttggttt tattgataga ggcgccaagt ttacgcatgg cctggctcat catacgagcc
    24241 tgcaaaccaa catggctatc tccgatatct ccatcaattt ccgcacgagg aacaagggca
    24301 gcaactgagt cgactacgac aagatcaact gcacctgagt caatcaattt tcccgcaatc
    24361 tcaagacctt gctctcctga gtctggttga gacaagagca attcgtcaat attgacacca
    24421 agggccgcag cataagctgg atcaagggca tgttccgcat cgataaaggc agcaatccca
    24481 ccttcttttt gcgcttgtgc aactgcatga agggcaaccg ttgtcttacc agatgactct
    24541 gggccataga tttcgatgat acgtccctta ggataaccac ctgagccaag ggcaatgtca
    24601 agagctaaag aacctgagct catcacttgc accttttgct ccgcacgttc acccaaacgc
    24661 atgattgatc ctttaccaaa gtctttctca atcaatttaa gagcgtcatt caaggccttt
    24721 tcacgttctg ccccaaattt ttttgaaatt tcttctaatt tttttggttt tttcgccatt
    24781 ctattctcct acattctaat gatcctcaga cctatctact attatatcaa aagttagtca
    24841 cttaataaag ccttgcgaac taggttaaag gcatgcataa ccgcaatgtg acgtacatct
    24901 gctcggcttc tgcctccaat attaaccttg ataacctcag ttccttgatc ttgcgccaag
    24961 cctatgaaga ctgtcccaac tgggtgtcct tctaggctat ctggtcctgc cactccagtc
    25021 aaactaattc caaaatcaga ctgggtcttg cttcgtgcct gctcagccat cttctgagct
    25081 gtaaattcag acaccacacc atgttcttcc aaattcttgg caggaatatc caacatcctt
    25141 gatttttcct ccaagctata ggtcacaaaa ccaccctcaa atatacttga aactccagaa
    25201 aaattcgcca cggtagcttg gaaaagacct gccgtcaaac tctctgcagc cgcgatggtt
    25261 ttcccttgcc ttttcagttc ttctaccaca atgctggcta aactagtttc ttccccataa
    25321 ccatagcaaa agtctcgtaa agaaattcct tcgaaagtct ggcagtccaa gatttgattt
    25381 tccaagatat ccagcgcttg attcgcctct tcttgactgc tagcctttgt tgacagacgt
    25441 agagtgactt ctcctgtctt ggcataaggg gccaaggtag gatcgatctg attatcaatt
    25501 aaatcagcca aaatcgtaac caactggctc tcgccaatcc caaagaaacg aagaactcgg
    25561 gaatacagct tgctccctgt catcaacttg ggtagaagtt ggtttaagac catgggtttc
    25621 aattcacttg gcggacctgg aaggacgaca taggtcactc cgtctacttc taattttcct
    25681 ccaacagcca gtcctgtttc gtttggcagt ggaatcgctc cttctacaat ttgagcttgt
    25741 ctttcgttat ttggtgttcg ggcatagtct ggtcgcaggg caaaaaagat atccaacttc
    25801 tcctgagcct gaggatcaaa gactaatgct ttccctaaaa atttagctag ggtttgtttg
    25861 gttaggtcgt cctcagttgg ccccaaaccg cctgtcaaaa tcaccagact gctacgttga
    25921 ctggcaatct caagcaaaga caagagacga acttcattgt ctcctacagc cgtctgaaaa
    25981 tatacatcta ccccaatctc agctagtttt tccgacaaaa actgggcatt ggtgttgaca
    26041 atctgtcctg tcaaaatctc tgttccaaca gcaatgattt ctgctttcat gtttcctcct
    26101 acctatctat tcgtattttt ttgaaaaaat cgcaggaatt ttcctacgat tgattttttt
    26161 atttgtatca aaagttaatt atcttcatca ccaacaggtg ctctgccaaa taaatcttca
    26221 aataaaaccg cattggtttc aagctgagta acttcttctt gtcccaaaga acgtcggagt
    26281 atattttgca tttccaacat atgtgctctc gaaacaatct ggtaagaaac accttgaagt
    26341 atctctcctt caccctgcaa ctgctgagtt tcaatggttt taaatgaatc tttatagcct
    26401 agcaagttag ggatactttt tgcagacaaa tcaatattgg tctgcatatt gtcactcaaa
    26461 gcttttagaa tctcttgata atgaccaacg ctatttaaac tgagagcttt ttccatgact
    26521 ttttgaataa cttcacgttg acgtttttga cgaccataat ccccctcagg atcttggtaa
    26581 cgcattcgtg catagactag ggcttcttct cccccaatat gttgctcccc aacaccgata
    26641 gaaatagtat taaattcttc ttggtcactg atagaaattg ggaaacctag gatattattg
    26701 actgtaatac ctcctactgc atccactagt ttttgcaacc ctctcatatt gaccatcaca
    26761 tagcgatcaa tatggatatt catcattttt tgaatggttt ctatagcaag ctctgctcca
    26821 ccatctgcat atgctgagtt cagtttcgct tcatgagcct gaccattccc tgattcaatg
    26881 cgcgtcagaa tatcccgctc taaactcatc attgttgttt ttttcgtttt aggattcact
    26941 gtcatcaaga tcatgctatc acttctaccg acccaagttt cagttcgttc agcatttccg
    27001 gtgtccactc ccattaacag aatggttaga ggttcagtcg cttcaataac cttggtttct
    27061 tcgccgattt ttttataggt tttagctaag gtttctgtcc cttgttgata aatagtataa
    27121 gcaaaaacac ctactcctac tacagttaca gaaagtaaag ctagcaccat tccaataatt
    27181 tttttaacca tatttctact aatctatcag tttacccatc aagtaaacat cgataaattt
    27241 cccttcttct atatatgccc cacgctcttg gctaccttca atgacaaagc catgcttttg
    27301 ataaagatgg actgctgctt gattacgagt ttggacagtc agttggagac gacgcagaat
    27361 gccacttgct tgtgcccact ctatcgcttc ttctagcaac aaacttccca agccattatt
    27421 ccaatatctt tttccaatca caatgaagag atctccaata tgacggactc tcttacgctg
    27481 atcagctgta atatttacaa taccagcaat tttgccattt aagaatgcaa gtaaggttat
    27541 ctgattgtcc gaactagctt gcttgttgag gaatatttcc atctcctcac tagtcaagag
    27601 aataccatct ccgtctaggc tggtaaagtc tgtctccaaa ctcacacgat ttaaaaaggt
    27661 cactaattca gctgcatctt tgggctctgc ttccctaatg agcaattcat actccatatt
    27721 gaagctcctc taacaatttc tcagcacgca aaccctttgc ctgaaaattt aaacggcgtc
    27781 catctgcttc ttttagaatt tccaattcta aataagcatc tggcaaggca tctcctaaga
    27841 gatttcccca ctcaataaca gtcacgccgc caccaaagat aaactcatcc aagtcgatag
    27901 aatcagcatc tccttcaata cgataaacat ctaggtgata aagtggaagt cgaccttcat
    27961 actctctcac gatagtatag gtgggacttt taatcatttg agaaatctgt aatccttttg
    28021 caagtccttt agtaaaggtc gttttacctg cacccagttc tccagttaag attaaaacat
    28081 cattctttgc taatagatgg cccaaacgct cccctaaggc ttgcaactct tcttcatttt
    28141 ttgtgtacat actcttatta taccaaaaac ttttcttttg tgtctatttt cctactaaac
    28201 ttatcatcat aacatccata aaaaacaggc tttctctaaa agaaaatgag cgtaacaatg
    28261 accaatacaa gatctcggaa aatatgacca taaaaggaaa cttccttctt aaccgaattt
    28321 gggacaagat aggctgcaaa aaacaagccc agtccagtat aaatcagaag tgagacaatg
    28381 gtcattggat ttcttaagaa aagaagtgtt gctaaaatag tcaccaacac tgtctttttt
    28441 ctgtctagca tagcaagaaa atcgcgcacg tattttttca agggtaaaaa aatcagcaaa
    28501 tctagcccaa ataggaaaaa gaaggatggc aataaaaagt caactaattc ttgctgcagc
    28561 gtatttttga tgaacaagtt atctgacaaa acaagaacag ctcctaacaa attaattaag
    28621 agtaacatac tgtaaaaaag cttcaccgac ttcttactgg ctaggacact atggacttct
    28681 tgcttacggg tataaagata atttactcca gcacagattc ctgaaacgaa aaccatgctt
    28741 ccgatgaaaa aagctgtact ttgtttaaag gacaagatgc attccttcca taggaaacag
    28801 ctactcaaac tgatttgaat taaagctaac aaaaataaga ttctcattga tttcatcttc
    28861 tctctccctt cctaccaatc attatactag gagaaaagag agaactgttt ctaatcttct
    28921 caaatgtctc tttaagacgc taaacaaaca ctagagacta atactcaatg aaaatcaaag
    28981 atcaaactag gtagctagcc acaggttgct caaaacagtg ttttgagatt gcagatagag
    29041 ctgacgtgat ttgaagagat tttcgaagaa tataaatttg aaatcatgaa aatccgtcaa
    29101 acgggtggtt gttttgtctc gcacctcacg gagcgagacg gactcagagt cacataatta
    29161 taaggctgat agtattaatc taactatcag ccttacaggt tatttaacg
//
LOCUS       DAYYQN010000021        29208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYYQN010000021 DAYYQN010000000
VERSION     DAYYQN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.021"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            83..1069
                     /locus_tag="ACHBRQ_000887"
     CDS             83..1069
                     /locus_tag="ACHBRQ_000887"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9592585.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            1133..1204
                     /locus_tag="ACHBRQ_000888"
     tRNA            1133..1204
                     /locus_tag="ACHBRQ_000888"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1166..1168,aa:Arg,seq:ccg)
     gene            1387..2085
                     /gene="rnc"
                     /locus_tag="ACHBRQ_000889"
     CDS             1387..2085
                     /gene="rnc"
                     /locus_tag="ACHBRQ_000889"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9592586.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            2076..5615
                     /gene="smc"
                     /locus_tag="ACHBRQ_000890"
     CDS             2076..5615
                     /gene="smc"
                     /locus_tag="ACHBRQ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9592587.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALDIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEKDRLSEGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            5612..6406
                     /locus_tag="ACHBRQ_000891"
     CDS             5612..6406
                     /locus_tag="ACHBRQ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9592588.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLGKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            6406..7224
                     /locus_tag="ACHBRQ_000892"
     CDS             6406..7224
                     /locus_tag="ACHBRQ_000892"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9592589.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            7228..8517
                     /gene="ftsY"
                     /locus_tag="ACHBRQ_000893"
     CDS             7228..8517
                     /gene="ftsY"
                     /locus_tag="ACHBRQ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9592590.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVDEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDSEPVVGVSQEEVEEFPHSEELPEEEKLDHE
                     GTIEENNSEVLEAERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVLEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(8563..10050)
                     /gene="zwf"
                     /locus_tag="ACHBRQ_000894"
     CDS             complement(8563..10050)
                     /gene="zwf"
                     /locus_tag="ACHBRQ_000894"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9592591.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTAATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(10199..10939)
                     /locus_tag="ACHBRQ_000895"
     CDS             complement(10199..10939)
                     /locus_tag="ACHBRQ_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9592592.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(10939..13104)
                     /locus_tag="ACHBRQ_000896"
     CDS             complement(10939..13104)
                     /locus_tag="ACHBRQ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9592593.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            13274..14227
                     /locus_tag="ACHBRQ_000897"
     CDS             13274..14227
                     /locus_tag="ACHBRQ_000897"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFM9592594.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            14304..16292
                     /gene="uvrB"
                     /locus_tag="ACHBRQ_000898"
     CDS             14304..16292
                     /gene="uvrB"
                     /locus_tag="ACHBRQ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9592595.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVQALD"
     gene            16302..16865
                     /locus_tag="ACHBRQ_000899"
     CDS             16302..16865
                     /locus_tag="ACHBRQ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9592596.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            16831..17319
                     /locus_tag="ACHBRQ_000900"
     CDS             16831..17319
                     /locus_tag="ACHBRQ_000900"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592597.1"
                     /translation="MTVIIKSMETPEEIEDKSLVHWQTWREAYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            17393..17860
                     /locus_tag="ACHBRQ_000901"
     CDS             17393..17860
                     /locus_tag="ACHBRQ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HFM9592598.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVIYYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(17857..18372)
                     /locus_tag="ACHBRQ_000902"
     CDS             complement(17857..18372)
                     /locus_tag="ACHBRQ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9592599.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(18384..18911)
                     /locus_tag="ACHBRQ_000903"
     CDS             complement(18384..18911)
                     /locus_tag="ACHBRQ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9592600.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPIVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(19194..19757)
                     /locus_tag="ACHBRQ_000904"
     CDS             complement(19194..19757)
                     /locus_tag="ACHBRQ_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9592601.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(19741..20292)
                     /gene="coaC"
                     /locus_tag="ACHBRQ_000905"
     CDS             complement(19741..20292)
                     /gene="coaC"
                     /locus_tag="ACHBRQ_000905"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9592602.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(20304..20993)
                     /gene="coaB"
                     /locus_tag="ACHBRQ_000906"
     CDS             complement(20304..20993)
                     /gene="coaB"
                     /locus_tag="ACHBRQ_000906"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9592603.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            21223..22893
                     /locus_tag="ACHBRQ_000907"
     CDS             21223..22893
                     /locus_tag="ACHBRQ_000907"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9592604.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            complement(22999..23124)
                     /locus_tag="ACHBRQ_000908"
     CDS             complement(22999..23124)
                     /locus_tag="ACHBRQ_000908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592605.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDRVSIIYLRQGEADVV"
     gene            23221..24396
                     /gene="mutY"
                     /locus_tag="ACHBRQ_000909"
     CDS             23221..24396
                     /gene="mutY"
                     /locus_tag="ACHBRQ_000909"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9592606.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            24451..25155
                     /gene="yycF"
                     /locus_tag="ACHBRQ_000910"
     CDS             24451..25155
                     /gene="yycF"
                     /locus_tag="ACHBRQ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9592607.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            25148..26497
                     /gene="vicK"
                     /locus_tag="ACHBRQ_000911"
     CDS             25148..26497
                     /gene="vicK"
                     /locus_tag="ACHBRQ_000911"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HFM9592608.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            26499..27308
                     /locus_tag="ACHBRQ_000912"
     CDS             26499..27308
                     /locus_tag="ACHBRQ_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9592609.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            27399..27641
                     /gene="relB"
                     /locus_tag="ACHBRQ_000913"
     CDS             27399..27641
                     /gene="relB"
                     /locus_tag="ACHBRQ_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9592610.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMIHDLGLKDE"
     gene            27643..27897
                     /locus_tag="ACHBRQ_000914"
     CDS             27643..27897
                     /locus_tag="ACHBRQ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9592611.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            27972..>29208
                     /locus_tag="ACHBRQ_000915"
     CDS             27972..>29208
                     /locus_tag="ACHBRQ_000915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFM9592612.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFE"
BASE COUNT         8902 a         5240 c         6609 g         8457 t
ORIGIN      
        1 ataataaatt aaggaaacgt cggaaaaggc gtagtgatgc gagagcatag gtaggtcatt
       61 acaaaagaaa cgagacatcg atatgttaaa tgaatttcca atttttgatt acgaagatat
      121 tcaattgatt ccaaataaat gtgtcattaa aagccgtgca gaagcggata caagtgtcac
      181 tttaggaaat cacaccttta aactacctgt tgtgccagcg aatatgcaga cgattttgga
      241 tgaaaatgta gcagagcaac tggctaaagg tggttacttc tacattatgc accgttttga
      301 tgaggcagga cgcattcctt ttattaaacg aatgcacaat caagggctca ttgcttccat
      361 atctgtcggt gttaaggact atgagtatga tttcgttaga cagctcaaga ctgatgctcc
      421 tgagtatatc actattgata ttgctcatgg tcatgcggat agcgtgattt ctatgattca
      481 acacatcaag aaagaattgc cagatacctt tgtcattgct ggaaatgtgg gaacaccaga
      541 agctgtgcgt gaattggaaa atgctggtgc ggatgctact aaggtcggaa tcggtcctgg
      601 taaggtttgt atcaccaagg ttaagactgg ttttggtaca ggtggttggc agttggctgc
      661 tctacgctgg tgtgccaagg ctgcgcgtaa accgattatc gctgatggag gaattcgtac
      721 tcacggtgat attgccaagt ctatccgctt cggtgctagc atgatcatga ttggttccct
      781 ctttgcagga catattgaaa gtccagggaa aacgattgaa gtcgatggtg aacagttcaa
      841 agaatattat ggttcagcct cacaatatca aaaaggtgct tacaaaaatg tggaaggcaa
      901 acgtatcttg cttcctgcta aaggtcatct gcaagacact ttaactgaga tggaacaaga
      961 ccttcaaagt gctatttctt atgcaggtgg acgtcaggtt gctgacctta aacacgttga
     1021 ttatgtgatc gtgaaaaact ccatctggaa tggggatgct tcccactaag acggggtctt
     1081 tgactaaaaa acttgttatt agagcaaatt tctgttataa taaaacaagt ttccaccctt
     1141 agtgtaatgg atatcacgta agattccggt tcttgagatg ggggttcgat tccctcaggg
     1201 tggatgtaaa cattctaaaa agcctttaat agggcttttt tctttacact actctaaatt
     1261 tgccactaaa actgagaatc tctgtctaaa gaattcccaa atgctttcca gtcccgtttt
     1321 agagtgactc agggggcttt ttttgatata ataaaaagga ctgttatcag ttagaaagag
     1381 gttggtatga aagaattaca aactgtacta aagaaccatt ttgcaatcga atttacagac
     1441 aaaaagttac tggaaactgc ctttactcat acgagttatg ccaatgagca ccgcctctta
     1501 aaaatttcac ataatgaacg cttggaattt ttaggagacg ctgttctaca gttattgatt
     1561 tcagaatatc tgtataaaaa atatcctaaa aagcctgaag gtgacctatc aaaactccgt
     1621 gctatgattg tccgtgagga gagtttagct ggttttgcgc gtgattgcca gtttgaccag
     1681 tttatcaagt tgggtaaagg ggaagaaaaa tctggtggtc gcaatcgtga caccattctt
     1741 ggtgatgcct ttgaagcctt tcttggtgcc cttcttttgg ataaggatgt ggccaaggtc
     1801 aaggaattta tctatcaagt catgattcct aaggttgaag caggcgaatt tgagatgatt
     1861 acagactata aaacccatct ccaagagtta cttcaggtca atggtgatgt ggctattcgt
     1921 tatcaggtga tttctgaaac agggcctgct cacgataagg tctttgatgt agaagttctt
     1981 gttgaaggta agagcatcgg tcaaggccaa ggtcgttcta agaaattagc agagcaggaa
     2041 gctgccaaaa atgccgttga gaaggggctg gattcatgta tttaaaggaa atcgaaattc
     2101 aggggttcaa gtcttttgct gataagacca aggtcgtttt tgaccaaggt gtgacggcag
     2161 ttgttggacc caatggatct ggaaagtcca atattacaga aagtctgcgt tgggctttgg
     2221 gggagtctag tgtcaagagt ctccgtgggg gcaagatgcc ggatgtcatc tttgctggaa
     2281 cagaaagtcg caaaccgctc aattatgctt ctgtagttgt gactctggat aatcatgacg
     2341 gatttatcaa ggatgcaggt caagaaatca gggtggaacg ccatatctat cgtagtggag
     2401 atagcgaata caagattgac ggcaagaaag tccgtctgcg tgatattcat gaccttttct
     2461 tggatacagg tttgggacga gattccttct ctattatttc ccaagggaag gttgaggaga
     2521 tttttaattc caagcctgag gagcgacgag ctatttttga agaagctgct ggagttttaa
     2581 aatacaagac tcgcagaaaa gaaaccgaga gtaaactgca gcaaactcag gataatctgg
     2641 accgcttaga ggacattatc tacgagttgg ataatcaaat caagcctctt gagaagcaag
     2701 ctgaaaatgc ccgtaaattt ctagacttag aaggtcaacg caaggctatt tacttggatg
     2761 tattggttgc tcaaatcaag gaaaataagg cagaactaga gtcgacagaa gaagagttgg
     2821 ctcaggttca agaactcttg atgagttatt accaaaagcg tgaaaaatta gaagaagaaa
     2881 atcaaactct taaaaagcaa cgccaagatt tacaggctga aatggccaaa gaccaaggca
     2941 gtttgatgga cttgactagt ctgattagtg atttagaaag aaaattagcc ctatcgaaac
     3001 tggagtccga gcaagtggcc ttgaatcaac aggaggcaca agctcgtttg gctgctttgg
     3061 aggataagag aaattcactc agcaaagaaa agtctgataa agaaagctct ttagctctgt
     3121 tagagggaaa tctagtccaa aataatcaaa aactcaatcg tttagaagct gaattgctgg
     3181 ctttctcaga cgatcctgat cagatgattg agctcttacg tgaacgcttt gtagctcttt
     3241 tacaagaaga agcggatgtc tcaaaccagt tgacccgtat tgagaatgag ttggaaaata
     3301 gtcgtcagct ttctcaaaaa caagcagatc aactagaaaa gctgaaagag caattagcta
     3361 tagctaaaga gaaggctagt cagcaaaagg aagagcttga aactgccaag gagcaggttc
     3421 agaaattatt ggctgactac caagctattg ccaaggagca agaggagcag aaaacttcct
     3481 atcaagctca acaaagccaa ctctttgacc gtctggataa tctcaaaaac aagcaggcca
     3541 gagctcaaag tttggaaaat atcctgagaa atcatagtaa cttttatgca ggtgttaaga
     3601 gtgttctcca agaaaaagat cgcctaggtg ggattattgg tgcagtcagt gagcatctga
     3661 cctttgatat gcattatcaa actgccctag acattgcgct tggagcgagt agtcagcaca
     3721 ttattgtaga agatgagaat gcggcaacca aagccattga tttcctcaaa cgaaacagag
     3781 ccggtcgtgc aacctttctt cctttgacca ctattaaggc gcgtacgatt tctagtcaga
     3841 accaagatgc tatcgctgta agcccaggtt tccttgggat ggcagatgag ttggtgactt
     3901 ttgatactag actggaagcc attttcaaga acttgctagc tacgacggct atctttgata
     3961 ccgtagaaca tgcgcgtgca gcagctcgcc aagttcgtta tcaggttcgt atggtgacat
     4021 tggatgggac agaattacgc acgggtggtt cctatgcggg tggtgccaat cgccaaaata
     4081 acagtatttt catcaagcca gaactggagc aattacaaaa agaaattgct gcagatgaag
     4141 caagcttgcg ttcagaagaa gtggctttga agaccttgca agatgagatg gccagattga
     4201 ccgagtcatt agaagccatc aaatctcaag gagagcaggc acgtattcag gagcaaggct
     4261 tgttcctcgc ttatcagcaa actagtcagc aagttgaaga actggaaact ctttggaaac
     4321 tccaagaaga ggaaaaagat cgtctttccg agggagattg gcaagcagac aaggaaaact
     4381 gccaagagcg tcttgctgca atcgccagtg acaagcaaaa tctggaagct gagattgaag
     4441 agattaagtc taacaaaaat gccatccaag aacgctatca aaacttgcag gaagagctag
     4501 cgcaagctcg tttgcttaag acagaactgc aagggcaaaa acgttatgaa gtgactgata
     4561 ttgaacgctt aggtaaggaa ctagataatc ttgatattga gcaagaagaa attcagcgcc
     4621 ttcttcaaga aaaggttgac aatcttgaga aagttgatac ggatttgctc agtcaacagg
     4681 ctgatgaagc caaaactcaa aaaacaaatc tccaacaagg tttgattcgc aagcagtttg
     4741 agttggatga tattgagggt caactggatg atattgctag tcatttggat caggctcgcc
     4801 agcagaatga agagtggatt cgcaagcaaa ctcgtgctga agcaaagaaa gaaaaggtta
     4861 gcgagcgctt gcgccatcta caaagtcaat taacagacca gtaccagatc agctacacag
     4921 aagctttaga aaaggcgcat gagttggaaa atctcaatct ggcagagcaa gaagttaagg
     4981 atttagagaa ggctatacgc tcactgggtc ctgtcaatat agaagctatt gaccagtacg
     5041 aagaagttca caaccgtctg gatttcctaa atagccaacg tgatgatatt ttgtctgcga
     5101 aaaacctgct tcttgaaacc atcacagaga tgaatgatga ggtcaaggaa cgctttaaat
     5161 caacctttga ggccattcgt gagtccttta aagtgacctt caagcagatg tttggtggag
     5221 gtcaggctga cctcattttg actgaaggag acttgctgac agctggggtt gaaatttctg
     5281 tccaaccacc aggcaagaaa atccagtctc ttaatctcat gagtggtggt gaaaaagccc
     5341 tatcggctct tgccttgctc ttctctatca ttcgagttaa gactatcccg tttgttatct
     5401 tggatgaggt agaggctgcg ctagacgaag ccaatgttaa acgtttcggg gattatctca
     5461 accgctttga taaggacagt caatttattg tcgtaaccca ccgtaagggg accatggcag
     5521 cggccgattc tatctatgga gtgaccatgc aagaatcggg tgtttcaaag attgtttcag
     5581 ttaagttaaa agatttagaa agtattgaag gatgacaatt aaactagtag caacggatat
     5641 ggacggaacc ttcctagatg ggaatggacg ctttgatatg gatcgtctca agtctctctt
     5701 ggtttcctac aaggaaaaag ggatttactt tgcggtggct tcgggtcggg gatttctgtc
     5761 tctaggaaaa ttatttgctg gtgttcgtga tgacattatt ttcatcgcgg aaaatggcag
     5821 tttggtagag tatcaaggtc aggacttgta tgaagcgact atgtctcgtg acttttatct
     5881 ggcaactttt gaaaagctga aaacgtcacc ttatgtagat atcaataaac tgctcttgac
     5941 gggtaagaag gggtcatatg ttctagatac ggttgatgag acctatttga aagtgagtca
     6001 gcattataat gaaaatatcc aaaaagtagc gagtttggaa gatatcacag atgacatttt
     6061 caaatttaca accaacttca cagaagaaac gttagaagct ggtgaagctt gggtcaatga
     6121 taatgtccct ggtgtcaagg ctatgacaac tggctttgaa tctattgata ttgttctgga
     6181 ctatgtcgat aagggtgtag ctattgttga attagctaaa aaacttggta tcacaatgga
     6241 tcaggtcatg gcttttggag acaatcttaa tgacttacat atgatgcagg ttgtgggaca
     6301 tcctgtagct cctgaaaatg cacgacctga aattttagaa ttagcaaaga ctgtgattgg
     6361 tcaccataag gaacggtcgg ttatagctta tatggagggc ttataatggc agatataaaa
     6421 ttgattgcat tggacttgga cgggaccttg ctgactactg ataaaaggct gacggatcgt
     6481 accaaggaaa ccttgaaagc tgcgcgtgat cgtggtatca aggtcgtatt gacaactggt
     6541 cgtcccttaa aagccatgga tttctttctc catgagttag ggactgacgg tcaggaagat
     6601 gagtatacca ttacttttaa tggtggatta gttcagaaaa atacaggaga aatccttgat
     6661 aaaacagtct tttcatatga tgatgtggca cgtttgtatg aagaaacaga gaaattatca
     6721 ctgcctcttg atgccatctc agaaggaaca gtttatcaaa tccaatcgga ccaagaaagt
     6781 ctttatgcca aattcaatcc agccttgacc tttgttccag tggactttga agacttatct
     6841 agtcaaatga cctacaacaa atgcgtgact gcctttgctc aagaaccctt ggatgcagcc
     6901 attcagaaga tttctccaga attgtttgac caatatgaaa tctttaaatc acgtgaaatg
     6961 ttgctagagt ggtcaccaaa gaatgttcat aaagcaacag gtttggcaaa actaattagc
     7021 catcttggaa tcaaccaaag ccaagttatg gcctgtggtg acgaggccaa tgacctctct
     7081 atgattgaat gggcagggct cggtgttgcc atgcaaaacg ctgttcctga agtcaaggca
     7141 gtcgcaaatg tggtaactcc tatgaccaac gatgaggaag ctgtcgcctg ggctatcgaa
     7201 gaatatgtgc taaaggagaa ctaagatatg ggattgtttg accgtctatt cggaaaaaaa
     7261 gaagaaccta aaatcgaaga agttgtaaaa gaagctctgg aaaatcttga tttgtctgaa
     7321 gatgttgatc ctaccttcac agaagttgat gaagttcctc aggaagaagc agaggttgaa
     7381 attgttgaac aagctgtgtt ccaagaagag gaaatccaag acacagttga agaaagtctg
     7441 gattcagagc cagttgtagg ggtttctcaa gaagaagtcg aagaatttcc acactcagaa
     7501 gaattaccag aggaagagaa gcttgatcac gaaggaacta tagaagaaaa taattctgaa
     7561 gttcttgaag cagaaaggcc tcaagcagaa gaaaccgttc aggaaaaata tgaccgcagt
     7621 cttaagaaaa ctcgcacagg tttcggtgcc cgcttgaatg ccttctttgc taacttccgc
     7681 tctgttgacg aagaattttt cgaggaactg gaagaactgc tgattatgag tgatgttggt
     7741 gtccaagtcg cttctaactt aacggaggaa ctacgttacg aagccaagct tgaaaatgcc
     7801 aagaaacctg atgcacttcg tcgtgtcatc attgagaaat tggttgagct ttatgaaaag
     7861 gatggtagct acgatgaaag catccacttc caagataact tgacagttat gctctttgtt
     7921 ggtgtgaatg gtgttgggaa aacaacttct atcggaaaac tagcccaccg ctacaaacga
     7981 gctggtaaga aggtcatgct ggttgcagca gataccttcc gtgcgggtgc agtagctcag
     8041 ctagctgaat ggggccgacg agtagatgtt ccagtagtaa ctggacctga aaaagctgat
     8101 ccagccagcg tggtctttga tggtatggaa cgtgccgtgg ctgaaggtat cgatattctc
     8161 atgattgata ctgctggtcg tctgcaaaat aaggataacc ttatggctga gttggaaaag
     8221 attggtcgta ttatcaaacg tgttgtgcta gaagcaccac atgaaacctt cttggcactt
     8281 gatgcatcaa caggtcaaaa tgccctagta caggccaaag aattttcgaa aatcacacct
     8341 ttaaccggaa ttgttttgac taagattgat ggaactgctc gaggaggtgt ggttctagcc
     8401 attcgtgaag aactcaatat tcctgtaaaa ttgattggtt ttggtgaaaa aatcgatgat
     8461 attggagagt ttaactcaga aaactttatg aaaggtctct tggaaggttt aatctaatca
     8521 gaagcaaaaa tcctgcaagg cataaacttg caggaaattt ttttattcta agcgaccatc
     8581 ttgacgatag gcgatatctg gttgccaagt ccatttggca ccgaattttt caagtaggtc
     8641 aaagctggct tgaggtccca tgcttccagc tttatagtca tgaagtgggg caccattttc
     8701 agcccagagc ttttcaatac ggtcaattaa cttccatgac gcaccaactt catcccagtg
     8761 gctaaagtta gttgagttgt tatttaggac atcataaatc aatttttcgt atggttctgg
     8821 agaagcacca gttgcagtcg cagctgtacg gtaatcaagt gagttaggag ccaagttaaa
     8881 ttcttctcct acttgcttcc catttaggct aagagagaag ccttctgttg gttgaatata
     8941 gatggtcaaa atatttggag caagtggttc tccaaatata gaatccattt gtttaaagac
     9001 gatgttgaca tgagttcctt tttcagtcag tcgtttacct gtacggaaaa agaaaggaac
     9061 actacggaat cgatcgctgt ctacaaagaa ggcaccagat gtaaaggttt cagttgttga
     9121 ttctggattc acatttggct cgctacgata agagatgtat ttcatgccat caatcttacc
     9181 agagcggtat tgcccacgga taaagtgttc tttgagttct tcatcagttg gatgatagag
     9241 gtttttaaag accttaatct tttcagcacg aatctcgtct tttgtgaagc ttgctggttt
     9301 gtccatggcg aggagcgaaa gaagttgtag agtgtggttt tggaccatgt cacggagggc
     9361 accggattgg tcatagtagc caccacgttc ttctacaccc aagcgctccg caaaggtaat
     9421 ttgaacattg tcgataaaat ccttgttcca aacgttttca aaaatcaagt ttgcaaagcg
     9481 aactgcaaag atgctttgga tcatttcctt accaagataa tggtcgatac ggaaaatttg
     9541 ttcttcgtca aatgttgcta ggagttcgtc attcaacttg cttgcagttg cgtaatctgt
     9601 accaaatggt ttttcaacga tcaagcgctc aaaacctttg ccatcgacaa tgttttcaga
     9661 tttgaggtgt ttggcaatgg ttccaaagaa ctgaggtgcc atagacaaga agaagagctt
     9721 attgtgttca gcttggtatt tttcattaag ctcagcttgt aattgacgca aagcaatata
     9781 atgttccgaa tcattgacat catggctttg atagtagaag tggctagcaa attcttgggc
     9841 ttgctcggta ctatctgcca aatcaaggat ggactcgaca actacagatt caaaatattc
     9901 cttactccaa ggtctacggg cagttccaat aacggcaaag tgcttggaaa gattgccgga
     9961 tttatatagt ctaaaaaggg aagggtagag cttgcgttta gccaggtctc cactcgcacc
    10021 gaaaattgta acaataacct tagatgacat ctagctacct aatttctatt tttattccta
    10081 gtcttgctag gtatgaggaa acctcatttc ataaacttaa ttctaacata attttccgaa
    10141 aaatcaaaaa ttccaatacg aggtagaaaa actgcaagga aatccttaca gtttgagttt
    10201 agacgtttaa gaccttatct aagaactctt tcagacgagg gtgttgtggg ttatcaaaga
    10261 tttggtcagg tgttccgtct tcaaggaact cgccatctgc agtaaagata acgcggttgg
    10321 caacctgacg agcaaatccc atctcatggg ttacgataat catggtcatg ccttgctcag
    10381 ccaattcctt cataacgtta agtacgtctc caaccatctc agggtcaagg gcagaagttg
    10441 gttcatcgaa gagcatgatg tctggattca ttgctaggcc acgagcgatg gccacacgtt
    10501 gtttttgacc acctgatagg ctatctggat tggcattagc tttatctgct agtccaacct
    10561 tttcaagcaa ctccattccc aattcctcag cttcttcctt agtcatcaac ttgtgctcaa
    10621 taggagcaaa ggtgatgttg tccaatacag acatatgagg gaagaggttg aagtgttgga
    10681 ataccatgcc gatattttca cggacgtggt caacattggt tgttttttca gttaaatcat
    10741 agccgttcac agtgatgtga ccgctagtga cttcttctaa aagattgagg ctacggagga
    10801 aagttgactt accagaacct gaaggaccga tgatacaaac aacatctcct tcatagaact
    10861 tagtcgtaat tccttttagg acttcatttt ttccatagtg cttgtgtaaa tcatttacat
    10921 caatttttaa ttttgccatt aacgaatcct cttttctaag cgtttcgcta gtctagtcaa
    10981 aagtgtgata attacaagat agaagatagc aaggattgca tacatcttga aactttggta
    11041 gttacgagca atgataatct taccagtttg gaagagttca accaaaccga tagcagatac
    11101 gatagttgta tctttaagag cgataacgaa ttggttgaca aagtttggca acatcaattt
    11161 agttgcttgt ggcaagataa tcttacgcat ggtttttcca taagagatac ccaagcttcg
    11221 gctggcttcc atttggccaa ctggaacggc ctgaatacca ccacgaacga tttcagcgat
    11281 ataagcagcc gcattgagtg agagggcaat tgttccagct acaaagtcgt taattgggct
    11341 ttgttggcct gtgatagact cgatgaagtt tggaattccc cagaagatga aggctgcaag
    11401 aatcatcaat ggaataccac gaataacgtc aacgaaaatc tcagagatga cgcgaagaga
    11461 tttgtatggg ctaacgctaa acataccgaa gataattccg atgacaatgg caatagcaaa
    11521 tgagataaga gctagagcaa gagtgatacc aagaccgcta aggagttgtt tgtagttgtt
    11581 ttgaagcaag ccccagagcg ttgtttcgtc aacagtactt gttgaagcag ttgaagattc
    11641 gctagctagg tatttgtcaa gaatcttttg gaattcaccg tttgctttaa ggtttgcaag
    11701 tccgttgttg aacatttcaa tcagttctgg atttgctcct tttttaacgg caaaggctgt
    11761 ttcaccgatt ggagttccag agattggagt tttcaatttt tgaccttggc tgatagaata
    11821 tttgagaaca ggttcatcat ccataacggc atcaatggca ccagtattta aactgtcata
    11881 cattgaagaa ccatcagcaa aggttttgat tttgtagccg tatttgcttt gattttctgt
    11941 taggaaggtt tgagaagcag ttccgttttt aacaccgact gtctttcctt ttagatcttc
    12001 ataagaagca atattgcttg attctttgac accaagaatg gtattagcag tgtagtatga
    12061 ttctgagaag tcaaaagttg ccttacgagc atctgtgaca gacataccag cgatgatacc
    12121 atcggcttga ccagcttgga cagcactgat agcagcatca aaaccagggt tggtgatttc
    12181 aatttcaaaa ccttggtctt tagcgattgc cttaatcaat tccatatcaa taccagtgta
    12241 ttggttgctt gaattttgga aaacaaaagg tgcaaaagaa gaatcgctgg caatgatata
    12301 tttagcctta acaggaatgg cttttaatcc tgcgagagta gttgtagttg gcactgctga
    12361 agatgatgaa gcagtccatt tcttgataat tttatcaaga ctaccatctt ttttcatttc
    12421 agacaaggct tggttaaatt cagtaaccag gtgctcgtat ttacttcctt ttttcacacc
    12481 gaaagcaaaa cttcctacag cttcaccatc catttcaata tggaggtctt gaccttggtt
    12541 aatggcatat tcgataacag gtttgtcatc catcatggca tcgatggcac cagcactcaa
    12601 gctgttgttc attaaatcac cagtgtcaaa tgttttaata gtaaagccgt atttatcttt
    12661 gattgtttca aggaaacgtt gagcggcagt tccgttttta acaccaacgg ttttgccagt
    12721 taattggtcg tacttgctaa ttttgtgtga ctttgtagta gcaatgacaa cttttgtatc
    12781 atagtaagta tcagacatgg tgaagacttt ttcacgttct ttagtctttg tcatccctgc
    12841 catgatagcg tcggcttgcc cagcttgaac cgcattgact gctgcgtcaa atccaggata
    12901 ggacatctga atgttccagc ctttaatctc agcgactttg ttaataatgt caacatcaat
    12961 tcctttataa gtttgatctg aatctttaaa ctcaaaaggt gcataggcgg tatcagaaac
    13021 aatcttaatc gtttctgctt tggcaatacc taatgagaaa attgggaata aaattagcaa
    13081 aaatgctaga aattttttct tcatttttag tctccttttc cgaatattct ctcattatat
    13141 cagaaaaagt aaaaaaaggc aaatttttat atttttcgtg tcagaaaata taacatcgat
    13201 tttttccttt ccttgaattg ctattaaaaa gtgctataat aatagtatat atagaagaaa
    13261 agaggacggg gatatgaaga acaaaagaat atttaaagac ttccaagctt caaaaatgag
    13321 tttaaacatt tacacaagcc ccttgttagc ctttgttttt gtcttcatag gagagtttgt
    13381 ggcttttact ttgtatggta ttggcttgtt agctctcatc ggacttgcta gaaattttgg
    13441 agaggctggt caaaatcttg caagctactt gcagaccttg catcagagct tgacggataa
    13501 aacaagtgac tttcgtttaa ttttaggatt actggccttt ggttttattc ttaacactgt
    13561 gttcagatgg acaagaaaag ttgagaaaag acctattcga accttgggat tttatagaga
    13621 gaatttcctc agcaatcttc tgaaaggatt tagtctaggc ctggcacttt ttcttctgac
    13681 cttgttaggt ttagtggtct taggtcaata tcgtttggaa tccattcact tgaatcctta
    13741 ttctcttgcc tttgtcgtct ttactatccc attttggatt ttacagggga cagcagaaga
    13801 agtggtggcc cgtgcttggc tacttcctca attggcctca agaaccaatc taaaactagc
    13861 tattcttata tctagcctgt tctttaccct gcttcatatg ggcaattctg gtctcacccc
    13921 tctatctcta gtaaatctct ttttattcgg agttgccatg gctctttacc ttctcaaaac
    13981 tgatacagtt tggggtgttg caggtattca tggtgcttgg aattttgctc agggtaatct
    14041 ctttgggatt ttagttagtg gtcaaccgtc aggaacgtct ctgatgacct ttttaccaca
    14101 aggcaatcaa gattggctat caggtggttc ttttggcata gaaggttcca ttatgacaag
    14161 tctggtatta ctactgctga ttgtctatct tgctaataaa ttaaagaaag aaaatgaaag
    14221 gatgtgactt cggtccgtcc ttttcttcgt gaaaatacta taagtatgct aaaataggaa
    14281 tagcacatgg agagaggatt cttatgatca atcacattac agataatcaa tttaaactag
    14341 tatcaaaata tcaaccatca ggagatcaac cccaagctat cgagcagttg gtggataaca
    14401 ttgagggggg agaaaaagct cagattctga tgggggcgac tggaacaggg aagacctata
    14461 ctatgagtca ggtcatttct aaagtcaata aaccaactct ggttattgcc cacaataaaa
    14521 ctctggctgg tcagctctat ggggagttta aggaattttt ccctgaaaat gcagttgagt
    14581 atttcgtatc ctactatgat tattaccagc cagaggccta tgtcccttct agcgatacct
    14641 atattgagaa ggatagttct gtcaatgacg agattgacaa gcttcgccac tcagctacct
    14701 cagccctttt ggagcgtaat gatgttattg tcgtggcctc agtctcttgt atctatggtt
    14761 tgggttcgcc caaggaatac gctgatagtg tcgttagtct ccgtcctggt ctagagattt
    14821 ctcgtgataa actcttgaat gacttggtcg atattcagtt tgaacgtaat gatattgatt
    14881 tccaacgcgg aagatttcgc gttcgtgggg atgtggtaga gattttccca gcttcccgag
    14941 atgaacatgc ctttcgagta gaattttttg gagacgaaat tgaccgtatt cgtgaagttg
    15001 aggctctgac aggtcaggtg ttgggagaag tggatcattt agcgattttc ccagcgacac
    15061 actttgtgac caatgacgac cacatggaag ttgccattgc aaagattcag gccgagttgg
    15121 aagaacaatt agctgtcttt gaaaaggaag gtaaactgct tgaagcccag cgtttgaaac
    15181 agcggacaga gtatgatatc gaaatgttac gtgagatggg ctataccaat ggggttgaaa
    15241 attattctcg ccacatggat ggacggagcg aaggagagcc tccttatacg cttctcgact
    15301 tcttcccaga tgatttcttg attatgattg acgagagtca tatgaccata gggcaaatca
    15361 agggcatgta caatggagac cgttcgcgta aagaaatgct ggttaattat ggtttccgtt
    15421 tgccgtctgc tttggacaat cgtcctctcc gtcgggagga gtttgagagt cacgttcatc
    15481 agattgttta cgtttcagcg acacctggtg actatgaaaa tgaacagacc gagacagtga
    15541 ttgagcaaat cattcgtcca acgggactct tggatccaga ggtggaagtc cgtccgacta
    15601 tgggacagat tgatgacctc ttgggtgaaa tcaatgcccg cgttgaaaaa aatgagcgta
    15661 cctttatcac aactttgacc aagaaaatgg cagaggattt gaccgactac ttcaaggaaa
    15721 tgggtatcaa ggtcaagtac atgcactcgg atatcaagac cttggaacgg acggagatta
    15781 tccgtgacct gcgcttgggt gtctttgatg tcttggtcgg aattaacctg ctccgtgaag
    15841 gaattgacgt tcctgaagtg agcctcgtag ctattctcga tgctgacaag gaaggtttcc
    15901 ttcgcaacga acgtggactc atccagacta ttggacgtgc tgctcgtaac agtgaaggcc
    15961 atgtcatcat gtatgcagac acggttaccc agtctatgca acgtgctatc gatgaaactg
    16021 cccgccgtcg gaaaattcag atggcctata atgaagagca tggtatcgta ccacagacaa
    16081 tcaagaaaga aatccgtgac ctgattgccg tgaccaaggc agttgctaag gaagaggaca
    16141 aggaagtcga catcaatagc ctcaacaaac aagagcgcaa agaactcgtc aagaaactag
    16201 aaaaacaaat gcaagaagcc gtcgaagtgc ttgactttga actggcggct cagatccgtg
    16261 atatgatgtt ggaagtccag gccttggatt aggggaatag tatgatttat ttaagaaagt
    16321 taaagagaga agatttgatg tctttatggg aaatggctta ttcacaactt aatccagttt
    16381 ggaaacagta tgatgctccc tattatgatg attatcagta tttttcaaat tttaaagaat
    16441 tcgaactaca aaaatcagaa tccattttaa gcaactcaaa tcgccttggt atttttgttg
    16501 atgataaact agttgggact gtttcgcgtt attgggtatg taaacaaaca agatggatgg
    16561 aattgggaat tggtatttat gataaaaaat tctggaacac tggtattggg aaagttgcta
    16621 tgttgcagtg gatagatagg acgtttcagg attacttgga gttggagcat ctgggtttga
    16681 caacttggtc agggaatatt ggtatgatga aacttgctga aaaattaaga atgaaaaaag
    16741 aagctcatat tccaaaagtt cgttattatc aaggtaaata ttttgatagt attaaatatg
    16801 gtattttgag agaagactgg gagaaaataa atgacggtta ttatcaaatc aatggaaact
    16861 cctgaagaga tagaagataa atcccttgtt cactggcaaa cgtggagaga ggcttatgat
    16921 gaccttttgc ctgcggaatt tcaggataca atgacattag aaagatgtcg attctttagt
    16981 caaaagtatc cagaaaatac attgattgcg atggatggcg tgaaggtggt tggttttatc
    17041 agttatggca attttcgtga tgagactatt caagctggtg aaattattgc tttatatgtt
    17101 ttaaaagact attatggaaa aggtatcgca caaaagttaa tgaaagcagc tttgactgct
    17161 cttaatcatt tttctgaaat tttcttatgg gtattgaaag ataacaagcg cgccattgct
    17221 ttctatcaaa aaatgggttt tacttttgat ggacaagaaa aaatacttga acttggaaag
    17281 cctataaagg aaaaacgaat ggtgttctat tctaaataat tttaaaaaag taaaagataa
    17341 tatctctggt agtaagtctg aaaatttaat aaattagaaa gcgagtaaat ttatgtcccg
    17401 ttcccaatta acaattttaa caaatatctg tctgattgaa gacctcgaaa ctcagcgcgt
    17461 ggtgatgcag tatcgcgccc ctgaaaacaa tcgctggtct ggttatgcct ttcctggagg
    17521 tcatgtagaa aatggcgagt ctttcgcaga gtctgtcatt cgtgaaatct atgaagaaac
    17581 agggttgact atccaaaatc ctcaacttgt cggcattaaa aattggccac tagatacagg
    17641 tgggcgctac attgtcattt attataaggc gactgagttc tctggtagcc ttcggtcttc
    17701 agatgaagga gaagtttctt gggtgcaaaa agaccagatt ccaaacttgg atctggccta
    17761 tgatatgcta ccattgatgg aaatgatgga agctcccgac aagtcagagt ttttctatcc
    17821 tcgccgtaca gaagacgatt gggaaaagaa aatcttctag tcttttacta aataacctag
    17881 ctgatccaag gcctcctcga tatagtggag gtcttgttgt gtttcggctt cgactaggtg
    17941 gtaatggatg ccatctgtca attcagatag aggtctaaag tcagaatgct cgacttgttc
    18001 tagaaaatgt tgcacatctc tgcgacaaga cagtttaagc aaggtttcaa tttctccata
    18061 aacgggatgg tcaatcaagg tattctggac atgcccacca ttatcgacaa tagcaaggag
    18121 ttcctgaccg atttcttcaa cttcatgttt cactttgaaa agtttgtgaa cataaggatt
    18181 ggtatcaatt tgcttataga tgtagccacg attggtggat aggataggag cgccatcggc
    18241 tctcaaaatt gcaatgtcct gcacaatgac ctgacgtgtg acatgaaaat gttccgccaa
    18301 actttggcca ttaagggctt taggagcttc tttcaagagt tggagcaggg cttgtttgcg
    18361 atcttttgtc atagcttttc cttttaacgg cgttttcgaa gcactttata gacagctagt
    18421 gctaatgtat agtctaccat actatggata attgtgccaa atccaactag tacaaataga
    18481 acatagaaca tgttttctac attggtaccc gaagttgcgt aaaaaaggac acaggctaat
    18541 acttcagcaa gggcatgaac aacagccaaa acaaaattga aaacccagga agattttggt
    18601 ttatctaggg tatcggggaa tttttgtagg taaagagctc ctaaagcacc aaaagatata
    18661 tgggaaaagg cccgaaaaac gataacaatg ggatagccag ccatcaaaaa tccaaaacta
    18721 gaggctagga tgacaaaaac tgccatcaag ggcgacaaga acatggctat aaaaatagcg
    18781 atgtggctcc ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg cataacaatt
    18841 ggaatcaaaa tcgcaatagc cgttaaaagg gctgtcattg tcataaattg tgtctttttc
    18901 cgtgtattca caagaatctc ctttttaact gcatatacac tagtatggta caataaacca
    18961 gacaataaag caagaattta cttgggttta tagatcattt tttagttaaa agttatagta
    19021 gattgaaact agaatagtcc acctctactt ctaaaacatt gttagaaatc gatttggctg
    19081 tcctgatcga tttgtcctgt tcttatttcg ttttactaat agtaaagatt tcattaaaaa
    19141 gaaactgtat agagcaaaat ctccaccttc aggtttgaaa gcggagattg tttttatttt
    19201 ttcaaggttt gtagtcgtgg aacaatggct agggttagaa ttgcagaaat gaccaattca
    19261 gcaattgaat ttgttgagat aacggttgcc agaagaagtt ggatatttcc attataaaca
    19321 tttccaaata ggaagaagat tcctccaagg acaaagatag tatttgtcaa ggaaccaagg
    19381 gccccagcta aaatcagacc agtcttgttt ttcatcagtt tatagactaa gtaaggagtt
    19441 aaaccaatca aaatacgtgg gacaatggca atgatagctg agtagatgtt tccgtttggt
    19501 acgaagggag agaagaggta gcttgtcggt agaatcgtaa tcgtgttaac cgtcaagcta
    19561 agtaatccca tcaaaaatcc aagtgtaacc ccaactcgtg gaccataaat aatgctggca
    19621 ataatgacag gaatatgaac aatggtcggt ttgattggaa atggaaaaag gttaaagata
    19681 agtgagctca gaaagtgtat cacgagcatg gtagcaaaaa agatagcaat gggtgcaata
    19741 ttagagcgtt ttttcatcga tagtttcctt tattctttct aaaataattg tgaggtcagc
    19801 taaagctcct cgtccgtggt ctccacaagc tagtagggat tccttaggag caatcagctg
    19861 atagccgtaa gtttctaatg ttttcagatt attctgagtt actggatggt catacatttt
    19921 tgtattcata gcaggagcta ttagtttggg aatatgactt ggtagggcta gagctgtact
    19981 ggttaccatg ttgtccgcaa atccgtgagc tagttttgca atagtgttag cagttgcagg
    20041 taccacgata aataaatctg ctttttttcc aagttcgata tgattgacct gatcaggata
    20101 gggttccttc atgacatcca agtggacagg attctgtgag agtacctgta gtgtcaaagg
    20161 ttggataaac tctgtagcag cctgagtcat taagacagtg acttgatggc cttgtttttt
    20221 tagagaactg actaaatctg ccgacttata agaggcgatt gaacccgtta cagccaagag
    20281 aatgtttgcc atagttttcc tttctaagaa tgataggctt gaattttttc aaggaggagt
    20341 tctgcaattt cttctttagt ctggactgtt tgaagctgat ttttctcaac aaatatagct
    20401 cggtgctgac ctgctgaaat ttgagtcagg tcattcgcga tgattaaatc tgcttgattc
    20461 ttgataagac tttttcgtgc aatgtcaacc agatgatctt cggtaacatc aaccagcagt
    20521 ttgaaaccaa tcagatgaat agtaggattc cattccttga ctagggatat gattttgggt
    20581 gtctttttaa ggaacaaaac ctgaacctca tcagttgaag aaatcttggc ctgatgattc
    20641 tgcttgctta aaaattcttt tagattggag ctagcctgaa cttcctcaag ccctgtcata
    20701 taaacaggag tgtagtcaga aacagccatt gagtggatca agacctgata atcctgaaca
    20761 cgttcttgca tttctattag aaggtccttg gtattggtaa tttctcgaat acttaggtta
    20821 ggatgaggct ctggcttcag agctcgtttt gtcgtaatta aacaaacttc atgccctgca
    20881 aaaagcaaag tctctgtgat aattttcccc aagtgacctg tagaatggtt agtgatagag
    20941 cggacgctat cgatagcttc actggtaccg cccgatgtaa ctaaaatttt catagcacta
    21001 ttgtacacaa aaataagcgg taagtaaaat gaaagtgata aatttttggt aaaaacgaag
    21061 atttattata gtttcaaact ataaagccat ataaaattta agaaaggact ctaaaagtct
    21121 taaaaacaga gatatttacg aacgtttaga gagttttagg atgttaaaaa tcttgttttg
    21181 tgatataatg aattatcata taagaggtta gaggaatttt gaatgaaaac agatattgaa
    21241 atcgcacaga gtattgagtt gaagccaatt gttgatgttg tagagaaact tggtatttct
    21301 tacgacgatt tggagttgta cggaaagtac aaggctaaac tcagctttga taaaattcgt
    21361 gcagttgaga gcaatccagt cggtaaattg atcttggtta ctgccatcaa cccaacacct
    21421 gcaggtgaag gaaagtcgac gcttaccatt ggtcttgcgg atgccttgaa caagattggc
    21481 aagaaaacca tgattgctat ccgcgaaccg tctcttggtc cagtcatggg gatcaagggt
    21541 ggtgctgctg gtggtgggta tgcacaagtt ctgccaatgg aagacatcaa cctccacttt
    21601 actggagata tgcatgctat tacaactgcc aacaatgccc tttctgcctt gattgacaac
    21661 cacttgcacc aagggaatga gctggaaatt gatcaacgtc gtatcctctg gaaacgcgtt
    21721 gtggacttga acgaccgtgc gctccgccat gtgactgttg gtcttggtgg tcctctaaac
    21781 ggtattccac gtgaggatgg ttttgatatt acagttgctt cagaaatcat ggcaattctt
    21841 tgcttggcaa cggacatcga ggacttgaaa cgtcgtttgg cgaatatcgt tattggttat
    21901 cgctatgacc gtacgcctgt ttctgtaggt gatttgcagg ttgagggtgc cttggctttg
    21961 attttgaagg atgctattaa gccaaacttg gttcagacaa tttacggtac acctgccttt
    22021 gtacatggtg gtccatttgc caatatcgct catggctgta actctgtttt ggcgacgaca
    22081 acagcccttc acttggctga ttacactgtt actgaagctg gttttggtgc ggatcttggt
    22141 gctgagaaat tccttgatat caagacacca aacttgccaa catctccaga tgcagttgtt
    22201 attgtcgcaa cccttcgtgc ccttaagatg aatggtggtg tggctaaaga cgctctgact
    22261 gaagaaaatg tagaggcagt tcgtgcaggt tttgctaact tgaaacgcca cgttgaaaat
    22321 atccgtaagt tcggtattcc agcagttgta gctattaacg aatttgtatc tgatacagaa
    22381 gctgaaattg cagtcttaaa agaactctgt gcctcaatcg atgtaccagt tgaattggct
    22441 agtgtctggg ctgatggagc agaaggtgga gtagcacttg ccgaaacagt tgttaagaca
    22501 attgctgaaa atccagctaa ctataaacgt ttgtacgata atgacctttc tgtccaagaa
    22561 aagattgaaa aaattgtcac tgaaatctat cgtggtagca aagtgaactt tgagaagaaa
    22621 gctcaaacac aaattgctca aatcgttcaa aacggttggg acaaattgcc aatttgtatg
    22681 gctaaaactc aatacagttt ctcagacaat ccaaatgcgc ttggagcacc agaaaacttt
    22741 gaaattacca ttcgtgaatt ggtaccaaaa ttaggtgcag gcttcatcgt tgccttaact
    22801 ggtgatgtta tgaccatgcc aggtcttcca aaacgtccag cagctctcaa catggatgtt
    22861 gaaagcgatg gaactgtact aggcttgttc tagtggattg aaattgactt taagtgagtt
    22921 tggaaataat atccaagctc attttttctc agactgcaag cagttcatca ataagattta
    22981 tactcttcga aaatcttttc aaactacgtc agcttcacct tgccgtaggt atatgatact
    23041 gactctgtca gttctatcca caacctcaaa acagtgtttt gagcaacctg cggctagctt
    23101 cctagtttgc tctttgattt tcattgagta taagtagata gaaaagttat atactatcta
    23161 ttgaaaatat tataaatcag atacaaagac aggtgaaacc ttctgtgtta tactagagat
    23221 atgttagatt tgaaagaata cggtatcgtc atgtggccgg aggagaaagt catttctttc
    23281 cgtgagaaac ttctcgcttg gtatgatgaa aacaaaagag atttgccttg gaggagaagt
    23341 aaaaatcctt atcacatctg ggtatctgaa atcatgcttc agcagaccag ggtggataca
    23401 gttatccctt actacgaaag atttttggac tggtttccaa ctgtcgaaag tctggcaact
    23461 gcgcctgagg agagtttact gaaagcttgg gagggcttgg gctattattc tcgagttcgc
    23521 aatatgcagg ctgcagccca gcagattatg actgactttg gtggccaatt tccaaatacc
    23581 tatgaaggaa tttccagctt gaaagggatt ggaccttaca cagcaggagc catttccagt
    23641 attgctttta acttgcctga gccagctgta gatggtaatg tcatgcgggt cttggcgcgt
    23701 ctgtttgaag tcaaccacga tattgggatt ccaagtaatc gaaaaatttt tcaggcaatg
    23761 atggagatct tgattaatcc agatcgtccg ggtgatttta atcaagcctt gatggactta
    23821 gggtctgata ttgagtctcc tgtaaatccc agacctgaag aaagcccagt caaggacttt
    23881 agtgcggcat atcagaatgg aaccatggac cgttatccaa ttaaatctcc taagaaaaag
    23941 ccggttccta tttatcttaa agccttagtt gtgaaaaatt ctcaaggaca atttttactt
    24001 gaaaaaaatg aaagcgaaaa gctcttggca ggtttttggc atttcccctt tatagaagtt
    24061 gataactttt cgcaagaaga gcagtttgac ctctttcatc aggttgcgga agaaagtgtg
    24121 aactctggcc ccagtccaga agagagtttc cagcaggact atgacctaga tgttgattgg
    24181 cttgatgttt gttttgatac agtccagcat gtttttagtc atcgaaaatg gcatgtacag
    24241 attgtagcag gtcaggtgag tgacttccat gatttttcag atagggaagt tcgttggctt
    24301 tcaccagaag aattcaagaa ttacccactt gccaaacccc aacaaaaaat ctggcaggct
    24361 tatgcacaag ccaacttaga cagtagccaa gactagctat tgtgtcttct gactattttt
    24421 tggtataata gtagagaaaa aggtgaacat atgaaaaaaa tactaattgt agatgatgag
    24481 aaaccaatct cggatattat caagtttaat atgaccaagg aaggttacga agttgtaact
    24541 gcttttaatg gtcgtgaagc gctagagcaa tttgaagcag agcaaccaga tattattatt
    24601 ctggatttga tgcttccaga aattgatggt ttagaagttg ctaagaccat tcgtaagaca
    24661 agcagtgtgc ccattcttat gctttcagcc aaagatagtg aatttgataa ggttatcggt
    24721 ttggaacttg gggcagatga ctatgtaacg aaacccttct ccaatcgtga gttgcaggcg
    24781 cgtgttaaag ctcttctgcg tcgttctcaa cctatgccag tagatgggca ggaagcagat
    24841 agtaaacctc aacctatcca aattggggat ttagaaattg ttccagacgc ctacgtggct
    24901 aaaaaatatg gcgaagaact agacttaacc catcgtgaat ttgagctttt gtatcattta
    24961 gcatcgcata caggtcaagt catcacgcgc gaacacttgc ttgagactgt ctggggttat
    25021 gactattttg gtgatgtccg tacagttgat gtgactgtac gacgtctgcg tgagaagatt
    25081 gaagatacgc ccagccgacc agagtatatc ttgacgcgcc gtggtgtagg gtattacatg
    25141 agaaataatg cttgatttac tgaaacaaac catttttacc agagatttta tctttatcct
    25201 gattttgtta ggtttcatcc ttgttgtgac cctcttatta ctggaaaata gacgtgataa
    25261 tattcagttg aagcaaatca atcaaaaggt taaagatttg attgcaggag attattccaa
    25321 ggttcttgat atgcaaggtg ggtctgaaat caccaatatt accaataatt tgaatgactt
    25381 gtcggaggtt attcgtctca ctcaggaaaa tctagaacaa gagagtaaga ggctaaatag
    25441 tattctgttt tatatgacag atggggttct tgcgaccaac cgtcggggtc agattatcat
    25501 gattaacgat acagccaaga agcaactggg gttggttaag gaagatgttc tgaatagaag
    25561 cattttggaa ttgctcaaga tagaagaaaa ctatgaattg cgtgatttga ttacccaaag
    25621 tccagaattg ttgctagatt cccaagatat caatggcgaa tatttgaacc ttcgagttcg
    25681 ctttgccttg atacgtcgag agtctggctt tatttcaggt ttggtggctg ttttgcatga
    25741 tacgacggag caggagaagg aagaacgcga acgaagactc tttgtttcca atgttagcca
    25801 tgagttacgg actcctctga ctagcgtaaa atcctatctt gaagccttgg atgagggggc
    25861 tttgtgtgaa actgtagcac cagactttat caaggtttct cttgatgaga ccaaccgtat
    25921 gatgcgcatg gtgacggatc tcctccatct ttcacgtatt gataatgcta ccagtcacct
    25981 agatgtggaa ctgattaact tcactgcttt tattaccttt atcctcaatc gttttgacaa
    26041 gatgaaagga caggaaaagg agaaaaaata tgagttggtg agagattatc ccatcaattc
    26101 tatctggatg gaaattgata cagataagat gacgcaggtt gtcgacaata ttttaaataa
    26161 tgctattaag tattcgccag atgggggtaa aatcactgtc agaatgaaga caactgaaga
    26221 ccagatgatt ttatccattt ctgaccacgg tttggggatt cctaagcagg atttaccacg
    26281 tatctttgac cgtttctatc gtgtggatcg tgctagaagt cgtgcacaag gtggtacagg
    26341 tctaggactg tctatcgcta aagaaattat caaacaacat aagggcttta tttgggccaa
    26401 gagtgaatac ggcaagggtt caacctttac cattgtgctc ccttatgata aggatgcagt
    26461 gaaagaagaa gtatgggagg atgaagtaga agactagaat gagtgaaata ggctttaaat
    26521 acagtatttt agcgtcgggt tccagtggaa attcttttta tctggaaacc tcaaaaaaga
    26581 agcttttagt agatgcaggc ttgtctggca agaaaattac cagtctgcta gctgaaatta
    26641 accgtaagcc agaagacctg gatgccatct tgattaccca tgagcattca gatcatatcc
    26701 atggagtagg cgttttggct cgcaagtatg gtatggatct ttatgccaat gaaaagacct
    26761 ggcaagctat ggaaaatagt aaatatcttg gcaaggtgga ttcttcgcaa aagcatattt
    26821 ttgaaatggg taaaaccaaa acctttggag atatcgacat cgagagtttt ggtgtaagcc
    26881 atgatgcagt cgcaccgcag ttctatcgct ttatgaagga tgataagagt tttgtcctct
    26941 tgacagatac aggttatgtc agtgaccgta tggcgggcat tgtcgaaaat gcggatggtt
    27001 atcttatcga ggccaaccat gatgtagaga ttttgcgatc aggttcttac gcttggcgac
    27061 tcaaacaacg aatcctatct gaccttggtc acctttctaa cgaggacggt gctgaagcta
    27121 tgattcggac gctaggaaat cgtactaaga agatttacct tgggcattta tctaaggaaa
    27181 acaatatcaa ggaactggct catatgacca tggtcaatca gctggctcaa gctgatctgg
    27241 gagtcggagt agactttaag gtttatgata cctcaccaga taccgcaaca ccattgacag
    27301 agatatagaa agaacgctga gaaggtgttc tttttatatt gactggacac ctaaaaagta
    27361 atacaatggt gttaccatta aaaaagggag cacaaaagat gactactatt acattaaaag
    27421 tttctgaagc tgataaaaca tttatgaaag caatggctaa gtttgaagga gtttcccttt
    27481 cggaacttat tcgcaccaaa actcttgaag ccctagaaga tgaatacgat gctcgtgtgg
    27541 cagatttagc ctatcaagag tatttagaag acttggaaaa aggagttgaa cccattactt
    27601 gggaagaaat gatccatgat ttaggcttga aggatgaata atttgtataa attagttcca
    27661 acaagacgtt ttatcaagca attgaaaaaa ttggaccgtt atacgcagaa gctaattaca
    27721 aactatttac aaaccaatgt tttggaagac ccaagacgac acggaaaggc tttggttggt
    27781 aatcgcgttg gtcaatggcg ctatagaatt ggtaattatc gagttatcgt acaaattgta
    27841 gatgatgaat tagtcgttgc tactctagaa gttggtcatc ggagagatat ttattgaatt
    27901 acttttttca agtaaatttt cattctttaa aatatgatat aatagggctt acaatataag
    27961 agaggttgct gatgacaata gatattagtg aagaaagttt ggctaaagaa tcggctgact
    28021 tattaaaaat tcttttaaaa gatcgaacga caaagaaatc aattgtttgg gctacgcatt
    28081 cttatgaatt gttaggaaag ggatttgctc caagtgatcg catcaatcca agtaaggtta
    28141 ctggaaactt tgccaacttg attcagccca gatctgaaaa atccaaatat gaacaaaaag
    28201 atcggacaaa aataagggca gaagtgttta caccaacgtg gttggtcgca aagcaaaatg
    28261 gatatgtaga atcgaaatta ggctcgttaa gtcttgagga gtatgtggac ttacgatggt
    28321 tagaggtaac ttgtggtgaa gcaccttata tggtaacgag atatgatacc gtaactggtg
    28381 aggaaattcc attatctgag cgagttggtt ttgttgatag aaaactgcaa cggattagtc
    28441 gtgaggtttc agatgaagtc acattttata aacttgtaaa agatgcttat catgcttcgt
    28501 atggttatga gtatcaagga gattctctcc ttttggcgcg tgagaatctt ttggcaactt
    28561 ttgaggacta ttatctagct aagactggaa atcaaccaac attagagcaa aagaaagaaa
    28621 ttgcgactat tatctcttac aatgtctttc agatggatgg attgaaaaaa agtagtcctt
    28681 attcagctaa acaaaagcaa tctcaacaat tgagtttgtt tgcggatgag ctagaagtcc
    28741 aagaagtaga agaatctaaa acccatatca aagactggaa aaagaataga atgataggtt
    28801 ttgagcgcct atctagcgag gaaagtgaaa tgaaatttga tgtcgtgata ggaaatcctc
    28861 cgtatcagga aacaggagaa gccagagatg agccgatata tcactatttt attgatgaag
    28921 cttataaaat cgcagataaa tctatactaa tcaccccagc aaggttttta tttaaagctg
    28981 gtcaaactcc taaaaattgg atggaaaaga tgcttgaaga caagcattta aaagttcaat
    29041 tctatgaact taaaagcggt aaagtattta caggaacgga tattaagggt ggagtagcaa
    29101 taacttatcg tgatgttgat aaagatttgg gaccaatcgg agtatttact atttttgaaa
    29161 ctttgaatgg tattgtaaaa aaaatcgaaa aacatccaga atttgaaa
//
LOCUS       DAYYQN010000022        29047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYYQN010000022 DAYYQN010000000
VERSION     DAYYQN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.022"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            171..547
                     /gene="tnpA"
                     /locus_tag="ACHBRQ_000916"
                     /pseudo
     CDS             171..547
                     /gene="tnpA"
                     /locus_tag="ACHBRQ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            729..791
                     /locus_tag="ACHBRQ_000917"
                     /pseudo
     CDS             729..791
                     /locus_tag="ACHBRQ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(935..1564)
                     /locus_tag="ACHBRQ_000918"
     CDS             complement(935..1564)
                     /locus_tag="ACHBRQ_000918"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM9592613.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(1574..2575)
                     /locus_tag="ACHBRQ_000919"
     CDS             complement(1574..2575)
                     /locus_tag="ACHBRQ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFM9592614.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(2606..3247)
                     /locus_tag="ACHBRQ_000920"
     CDS             complement(2606..3247)
                     /locus_tag="ACHBRQ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFM9592615.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEILAK"
     gene            complement(3266..4081)
                     /locus_tag="ACHBRQ_000921"
     CDS             complement(3266..4081)
                     /locus_tag="ACHBRQ_000921"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9592616.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGEANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            4352..4786
                     /locus_tag="ACHBRQ_000922"
     CDS             4352..4786
                     /locus_tag="ACHBRQ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9592617.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            4798..5988
                     /locus_tag="ACHBRQ_000923"
     CDS             4798..5988
                     /locus_tag="ACHBRQ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9592618.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            5999..6490
                     /locus_tag="ACHBRQ_000924"
     CDS             5999..6490
                     /locus_tag="ACHBRQ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9592619.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            6505..7284
                     /locus_tag="ACHBRQ_000925"
     CDS             6505..7284
                     /locus_tag="ACHBRQ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9592620.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            7271..8089
                     /locus_tag="ACHBRQ_000926"
     CDS             7271..8089
                     /locus_tag="ACHBRQ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9592621.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            8089..8382
                     /gene="yajC"
                     /locus_tag="ACHBRQ_000927"
     CDS             8089..8382
                     /gene="yajC"
                     /locus_tag="ACHBRQ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9592622.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            8404..10305
                     /locus_tag="ACHBRQ_000928"
     CDS             8404..10305
                     /locus_tag="ACHBRQ_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HFM9592623.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            10365..11366
                     /locus_tag="ACHBRQ_000929"
     CDS             10365..11366
                     /locus_tag="ACHBRQ_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9592624.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(11476..11811)
                     /locus_tag="ACHBRQ_000930"
     CDS             complement(11476..11811)
                     /locus_tag="ACHBRQ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592625.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(11822..12064)
                     /locus_tag="ACHBRQ_000931"
     CDS             complement(11822..12064)
                     /locus_tag="ACHBRQ_000931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592626.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(12080..12274)
                     /locus_tag="ACHBRQ_000932"
     CDS             complement(12080..12274)
                     /locus_tag="ACHBRQ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9592627.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            12440..13390
                     /gene="rsmH"
                     /locus_tag="ACHBRQ_000933"
     CDS             12440..13390
                     /gene="rsmH"
                     /locus_tag="ACHBRQ_000933"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9592628.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            13402..13719
                     /gene="ftsL"
                     /locus_tag="ACHBRQ_000934"
     CDS             13402..13719
                     /gene="ftsL"
                     /locus_tag="ACHBRQ_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9592629.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            13723..15975
                     /gene="pbp2X"
                     /locus_tag="ACHBRQ_000935"
                     /gene_synonym="pbpX"
     CDS             13723..15975
                     /gene="pbp2X"
                     /locus_tag="ACHBRQ_000935"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HFM9592630.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVIEQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPSGEYFNSSELKIADATIRDWDVNDGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAMSAF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISALYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMILVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAEIADEKNGGYLVGST
                     NNIFSVVAMNPAENPDFILYVTVQQPEHYSGIQLGEFTNPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFEGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            15977..16957
                     /gene="mraY"
                     /locus_tag="ACHBRQ_000936"
     CDS             15977..16957
                     /gene="mraY"
                     /locus_tag="ACHBRQ_000936"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9592631.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLVLQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            17566..19671
                     /locus_tag="ACHBRQ_000937"
     CDS             17566..19671
                     /locus_tag="ACHBRQ_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9592632.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQNKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLNPFFRPELLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            20132..20467
                     /locus_tag="ACHBRQ_000938"
                     /pseudo
     CDS             20132..20467
                     /locus_tag="ACHBRQ_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(20534..21016)
                     /locus_tag="ACHBRQ_000939"
     CDS             complement(20534..21016)
                     /locus_tag="ACHBRQ_000939"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9592633.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(21111..22595)
                     /locus_tag="ACHBRQ_000940"
     CDS             complement(21111..22595)
                     /locus_tag="ACHBRQ_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9592634.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            22748..24355
                     /locus_tag="ACHBRQ_000941"
     CDS             22748..24355
                     /locus_tag="ACHBRQ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9592635.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            24520..24687
                     /locus_tag="ACHBRQ_000942"
     CDS             24520..24687
                     /locus_tag="ACHBRQ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HFM9592636.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(24680..25189)
                     /locus_tag="ACHBRQ_000943"
                     /pseudo
     CDS             complement(24680..25189)
                     /locus_tag="ACHBRQ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25440..26885
                     /gene="cps4A"
                     /locus_tag="ACHBRQ_000944"
     CDS             25440..26885
                     /gene="cps4A"
                     /locus_tag="ACHBRQ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HFM9592637.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKLSQNTDLT
                     VDQSSSYLVAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALSFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMIDLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            26887..27618
                     /gene="cps4B"
                     /locus_tag="ACHBRQ_000945"
     CDS             26887..27618
                     /gene="cps4B"
                     /locus_tag="ACHBRQ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HFM9592638.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLGKKRIPTLNDS
                     RYALIEFSMNTLYRDIHSALNKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRVQYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            27627..28319
                     /gene="cpsC"
                     /locus_tag="ACHBRQ_000946"
     CDS             27627..28319
                     /gene="cpsC"
                     /locus_tag="ACHBRQ_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HFM9592639.1"
                     /translation="MQDQNTLEIDVFQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            28329..29012
                     /gene="cps4D"
                     /locus_tag="ACHBRQ_000947"
     CDS             28329..29012
                     /gene="cps4D"
                     /locus_tag="ACHBRQ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4D"
                     /protein_id="HFM9592640.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILVTATGEANKRDVQKVKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
BASE COUNT         8826 a         5238 c         6469 g         8514 t
ORIGIN      
        1 tttgttcgta tcctatttca ctccactata ttagtccgtc tcgctccgtg aggtgcgaga
       61 caaaacaacc acccgctata cgggtgcgcg tcgaaggtta taccaaaaaa actccaaacg
      121 cgatacaata aaggtgttca atccacttgt aaagcgaaag gagaaaaaat atggcacaaa
      181 aggctcatag tttatctcac acaaagtgga ggtgttctat cacattgtgt tcacccctaa
      241 gtatagacga aaagtcatct ataatcaata tagaagtagt ttaggcgaaa tatttcatcg
      301 cttgtgtagt tataaaggtg ttgaaatgat cgagggtcac ttaatgccag accatgtaca
      361 catgttagtc agtattccgt cgaggctaag tgtttcaagt tttatggggt atttaaaagg
      421 caaaagtgca ctcatgatgt ttgacaaaca cgccaatctc aagtacaagt ttgggaatcg
      481 gcatttctgg gcggaaggtt attatgtaag tccagtaggg cttaatgaag ccacaattaa
      541 gaaatatagt caagattaaa gaaattatcc agtggatgat ttcttcacga gtatgaaaat
      601 ttgagaacga gtaaagtatg atatatagta aaatgaaata agaacaggac aaaatcgatc
      661 aggacagtca aatcgatttt taacaatgtt ttagaagtat aggtgtacta tttcagtttc
      721 aatctactat agcactagat aaattgagtg taaaagaata tgaggatccc tttagggata
      781 gtggtaagta ataccaaagt ctcttaaaga ggcaagtgac gagtcaagag caataaggct
      841 tgaacaacgt gaaagccagc gtctttaggc gctggctgat gatttgggct tatagctctg
      901 agataaacca cccgttagac gggtagttat gattttatct gagtgtaata tactgttggg
      961 caatctcgct gatgcggtca aagttgcctt gggaagcgag tttattgagt tcgccaccaa
     1021 ttccaacggc atctgcacca gcagcgaacc attgagggat gttgtttagg ccgactcctc
     1081 cggttaccat tacggaaact tgtgggatcg gtgccttgac tgcagagata tatgctggac
     1141 tgagagtact acctgggaag agtttgatga tttcactacc ggcttcaagt gcagtcgtga
     1201 tctctgtgag ggtaatacag cctggaatgt acggtgtgct gtagagattg cacattttcg
     1261 cagtttcagc atggaaagat ggagaaacaa cgtaatttgc tccagctaga atggcatctc
     1321 tagcagttac ggcatcaagc acagtacctg caccgataca aacactctga tcgtcctgat
     1381 acaagtctac aagttccttg atgatttgtc ctgcatactg attggtatag gcgatttcaa
     1441 tagctttgat accgcccttg atacaagcaa tcgaggcttg tagtccttct tcctttgtat
     1501 ttcctcgaat gacagcgaca attttctgtt tttttagttc aataatcgta tctgatttgg
     1561 tcatgtaatt ctcctaacga atgatatctt gtgcatttgc cagtaaattt tcaatactag
     1621 ttgcggaagt ggagagatgg tctcctggaa gagtgcattt gagagttgcg ctcgcaactg
     1681 caaagtcaat ggtagttttt agggaggaat gatggagtag ttggtaaagg gcaccagata
     1741 taaaggcatc cccgctacca attcgttgat agactgcagt ttttagttgg acagactctt
     1801 caaatcgttc ttccagagca taggcttgat agacattttt gtcttgctca tcactagatc
     1861 ggagggtatg gaaaatagcc ttgaaaccat aggcttcttt taaaagtcgc atgcgatttt
     1921 ccacctcttc taggctagca ctgtctcttg ggaacatctc tagattttgg tcatcaatca
     1981 tgagaggatc aataccgaag caatagtcag taaaacgtgc aaacttagaa aattcatact
     2041 tggcttctag gactgaaatc atctttgttc tcagattgag atccattgaa acgacaattc
     2101 ctcggcgctt ggcttcttcc aagagtagga gaaggatcgc acggacctct tgaccgatag
     2161 ctatggtgat tccactaaaa tgaaaatggc taatcccctg aaagagagaa tccatatcta
     2221 gcatgtttgg ccgaatctgg ctgatactcg tatgcttacg atcgtagaaa acttcacttt
     2281 gacgacaacc aaagccgttc tccaaatagt agaggccgat tcgatcgcca agccgacaga
     2341 ttgaactggt atcgatttgg tgctgtttca agaatgtgag aaaacgatct ccaatctcgt
     2401 tggcagggag tgcggtaaag actttcgttg agatacccag ggcttgcaaa ttacaagcga
     2461 tgttaatttc tgatccgcca aaataagtcg aactggcaac atgatcgccg atactggtgg
     2521 catctaatgg tgagattcga attaatggtt cgccaaacag taatattttc gtcatccatt
     2581 agttcctttc tggtttagaa tagctttact tggctaatat ttctttcaaa taagcagcga
     2641 cttcatcatc ttggcagttt tcaaagaagt attcttggaa gtatttgcca gagatggtgt
     2701 ctttgaggaa gtcttggtcg attgttttaa ggatggtcat caaatcattg tgggtgattt
     2761 gtttcacctc gtttaagata cgagcgttgc gttgttcagg gattacacgt tctcttgggt
     2821 agccaccgcc catttcttca gcaaataagc gttcaaacat caatttgagg gtcagttctg
     2881 ccccccagcc aaatcctttg gcataaggga tagacaaggc gttaccacca ttgatttgag
     2941 aataaaggta agcgtcagtt gggtccactg ctagaccaca gacaacacca gggaagctgt
     3001 ttaaagcaag catagcccct acacccgtac cacagccggt aacaacaaag tcaactgcct
     3061 ttgtatttaa aaggatggca gccattagtc cgttctgcac ataagttaat tgactttctc
     3121 cttcttctcc acgcatacca tagttaaata attggtagcc ttttttatct gtcgcttctt
     3181 ttagactatc gtaaataatg tgattcttgc tagcttgact attttcattg attaatgcga
     3241 ttttcataag atcttctttc tatttttact caggttgttt tccgatgtag gctaagatac
     3301 cgccatctac atataggatg tggccattga caaaattgct ggcatcacta gcgagaaaga
     3361 cagcagggcc catcaaatct tcaggatttc cccaacgtgc agcaggtgtt tttgcaatga
     3421 tgaactggtc aaatgggtgg cgagaaccat cttcttgcaa ctcacgaaga ggtgctgttt
     3481 gaggagtggc aatataaccc ggtccaattc cgttacattg gatattggct tcaccgtatt
     3541 cagacgcaat gttgcgggtc aacattttca agcccccttt agcagcagca taagcgctaa
     3601 ctgtttcacg tcccagttcg ctcatcatcg aacaaatatt gataatcttt ccatgccctt
     3661 tctttatcat agaaggaata actgcctttg aaacgataaa tggtgcgttt aagtcaatat
     3721 cgatgacctt acggaaatca gcggcgctca tttcgcacat tggaactcgg cggataatac
     3781 cagcgttatt aacgaggatg tcaatgacac caacctcttg ttcgatttgc ttgaccatgg
     3841 cttggatacc gtcctcgtct gtcacgtcac agacatatcc atgggcttcg atgccaactt
     3901 cacgataagc ttcaatccct ttattgacca gatcttgatt gatatcgtta aagacaatag
     3961 tagcgccggc ttcagcgtag gatttggcaa tagcaaatcc aattccataa gaagcgccgg
     4021 tgatgagagc aatttttcct tttaaagaaa actgctcaat tgagaatgat gtatttgtca
     4081 tttgatactt cctttcaaat ttgttaagac ttctctataa ccaaattata ttctagtaaa
     4141 ccggttttgt caataaataa atatttttat ttataaaaag atttattttc cttaaaaatg
     4201 gaatattttg aaatttctgt ctattagaat gattaaaaag ataatgaaag cgctaatatt
     4261 ttttaaaaaa aactattgac aagataaaaa taaaggatta caatggggaa tataaagtaa
     4321 accggtaaac ttaaacaaga aaggagaaaa gatgaaaatt gtacttgtag ggcatggaca
     4381 ttttgctaca gggatttata gttctttaca attgattgca ggcaatcaag aaaatgtgga
     4441 ggcaattgac tttgtggaag gaatgtcagc agatgaactc aagcaaaaaa tcttacttgc
     4501 aatttcaaat gaagaagaag ttttaatcct aagtgatctc ttgggaggat cgccattcaa
     4561 ggtttcttct accataatgg gagaaaatcc agccaaaaca atgaatgttc tttcgggttt
     4621 gaacttagcc atgttaatgg aagcagtctt tgctagaatg gctcatagct ttgatgaggt
     4681 tgttaataaa tcagtagtgg cggcccaggg cggagtcgta aatggtaaag aattgttttc
     4741 aacggatgca gaggaagagg aagaagattt cgaatcgggt atttaaaggg taaaagaatg
     4801 ataaaaaagg ttacgattga aaaaataaaa tcgcctgagc gcttcttaga agtaccactt
     4861 ctgacgaaag aagaagtcgg ccaggcaatc gataaggtta ttcggcagtt agaactcaac
     4921 cttgactatt tcaaggaaga tttcccgacg ccagctacct ttgataatgt ctatccaatc
     4981 atggataaca cggaatggac caatggtttc tggacaggag aactgtggtt ggcttatgaa
     5041 tacagtcaac aggatgcatt taaaaacatc gctcataaaa atgttctttc tttcctggat
     5101 cgtgtcaata agagagtaga attggatcac catgatctcg gcttcttgta cacaccgtct
     5161 tgtatggctg aatataagat aaatggagat ggagaggcta gagaagcaac cttgaaagct
     5221 gcagataagt tgattgaacg ctatcaagaa aaaggtggtt ttattcaagc ttggggagac
     5281 ttgggcaaga aagagcatta ccgtttgatt atcgactgct tgctcaatat ccaactctta
     5341 ttctttgctt atcaagaaac aggcgatcaa aaatactacg atattgcaga aagtcatttc
     5401 tatgcttcag ctaataatgt aatccgtgat gacgcttcgt ccttccacac cttctatttt
     5461 gatcctgaga caggtcaacc ctttaaaggt gtaacgagac aagggtatag tgatgattca
     5521 tgctgggcac gtggtcaatc atggggagtc tatggtattc ctttgactta tcgtcactta
     5581 aaagacgagt cctgctttga cttgtttaag ggtgtgacca attatttctt gaatcgtctg
     5641 ccaaaagatc atgtgtccta ttgggatttg atttttaatg atggtagtgc tcaatcacga
     5701 gattcttcag caacagctat cgccgtctgt gggattcatg aaatgctaaa acatctccca
     5761 gaggtggatg ctgacaaaga tatttataaa catgctatgc atgccatgct tcgttccttg
     5821 atcgaacatt atgcaaatga tcaatttacc cctggtggga caagtctcct ccacggtgtg
     5881 tactcatggc attcaggtaa aggagtggat gaaggcaata tctggggtga ctactattac
     5941 ctagaagccc ttatccgttt ctacaaagac tggaacctat attggtagga ggagaaatat
     6001 gacaatgcca aatattatta tgacccgtat cgatgaacgg ttgattcatg ggcaaggaca
     6061 actttgggta aaatacctag gttgtaatac ggtcattgtt gccaatgacg aagtaagcac
     6121 ggacaagatg caacaaactc tgatgaaaac agttgtgcca gactcagttg ccatgcgttt
     6181 cttccctttg caaaaggtga ttgatatcat tcacaaggct aatcctgctc aaacgatctt
     6241 tatcgttgta aaggatgtga aggatgcttt aaccttggta gaaggtggtg tccctatcaa
     6301 agaaatcaat attgggaaca ttcacaatgc tactggtaaa gagcaagtga cacgctccat
     6361 cttcctgggt gaagaggaca aggcggccct caaggaattg agccaaactc atcaagtaac
     6421 atttaatacg aaaacaactc caacaggaaa tgatggagct gttcaagtca acattatgga
     6481 ctatatttaa aagaggagat cgttatgtcg attaatgtat ttcaagcgat tttaattgga
     6541 ttatggacag ctttctgttt tagtggaatg ctgttaggaa tttacaccaa tagatgtatt
     6601 gttctgtcat ttggtgtcgg aattattcta ggtgatctgc ctactgctct tgcaatggga
     6661 gctattggtg aattggctta tatgggattc ggtgttggtg ctggaggtac tgttccacca
     6721 aacccaatcg gacctggtat ctttggtacc ttgatggcta tcactagtgc tggtaaagtc
     6781 agtccagaag cggctcttgc cctctctact ccgattgctg tggcgattca attcttacaa
     6841 actttcgcct acactgtacg tgctggtgcg cctgaaacag cgatgaagca cttgaaaaac
     6901 cataatttga agaaattcaa gttcactcta aatgcaacaa tttggttgtt tgcctttatt
     6961 ggatttacct tgggttgctt gggtgccctt tcaatggata ccttgttgaa actcgtagac
     7021 tacattccac cggtattgct tacaggtttg acagttgctg gtaaaatgct cccagctatc
     7081 ggttttgcga tgatcttgtc agtgatggct aagaaagagt tgattccctt tgtcttgttg
     7141 ggatatgttt gtgcagctta tctaaacatc ccaacaattg gtattgcaat tgtaggtact
     7201 atctttgctt tgattgaatt ttataacaag ccaaaaacag cggatcatgt ggtagaggag
     7261 gaagcacacg atgactggat ctaacaaatt aacaaaacgt gattatctta aaacgtcttt
     7321 gcgggcattc ttttgtcaaa atggatttaa ctatagtaac tatcaagggt tgggatatgc
     7381 caatgtgatg tatcctgctt tgaaaaaaca ctatggagag gatcaggaag gtttctacca
     7441 agccttggaa gaaaactgtg aattctataa taccaaccca cacttcctgc cttttattac
     7501 cagcttgcat cttgtaatgt tggaaaatgg ccgtccggca aaagaaacac gtagcatcaa
     7561 gatggccttg atgggaccat tggcaggtat tggggattct ctttctcaat tctgtttagc
     7621 tccattgttc tcaaccatcg cagcttcgtt tgctcaagaa ggcttggttg tcggtccaat
     7681 cttgttcttc cttgcgatga atacgatttt aacagcgatt aaattgtcaa ctggtctgta
     7741 tggatacaaa ctaggaacaa ctgtgattga taaactaagc gaacagatgg caacgatttc
     7801 tcgtattgcc aatattatcg gtgtaaccgt aattgctggt ttggcagcga catctgttaa
     7861 gattatggtg ccgattacct ttgctgcagg ggaagttaaa gcagacgcta aacaaagtat
     7921 cgtaagtatt cagggaatgc ttgataaggt tgctccagct cttctaccag ccctatttac
     7981 acttttagtt tactacttga tcaaagaaaa gaaatggaca acatataaac tcgttatttt
     8041 aacagttatc atcggaatta tcggaagctg gcttaagatt atagcttaat ggatacaaca
     8101 ttgttttatg gaatagtgat tgtcttggca gtgagtcccc ttttactgtc aagctttcat
     8161 tctattcgtc agcaaaagtt gcttcgcaaa cagatggagc aacgacaaga gtatttagct
     8221 tctttaacat ctggtgatga agtgttgtta ttgtctggaa ttcatggaaa aatcatttct
     8281 atccaagatg acttggtctc cttgcagatt gcaaaaggtg tggtcatcta tgtagaaaag
     8341 gaaagtgtaa tgggaaagac aaaagaactg ctttttaagt agttcttttt ctggtaatag
     8401 ataatgaaag aagaaagaag acaatttttt gaaagagtcg atggaaacca atgtcgtgat
     8461 tatatcttgt cccactgttc aaaagactat gagaaagtca agagttccct tgaacgcttg
     8521 atagacaatc gttttatgtt tgatagtcct tgggatatgg agccttgttc aaaaatccat
     8581 caaatccagc cgatggtatg ggatcaagta tttgaagatg atccagaatg gtcttatatg
     8641 ctcaatcgac aagaatatct cttgcagttt atgatagggt atctggtaga aggagataag
     8701 ggctatatcc aaaagtgcaa gttctttcta tttgattgga ttgagcaggt gagagaattt
     8761 tctcctcaat ccttgatgac tagaaccttg gatacgggta ttcgttcctt tacttggttg
     8821 aaactactct tgctcctctt gaaatttgac ttgctagagg agaaagaact agagaaaatt
     8881 ttggtcagtc tagaaaagca gattgacttt atgaaaagcc actatcgcgc caagtacacc
     8941 cttagtaact gggggatttt acaaacaatt ccgatgcttg ctatctatta tttcttttca
     9001 gataagatgg acctagaaga agcttaccat tttgcttcag aggagttgaa acagcaaatt
     9061 gagacacaga ttttaggaga tggaagccag tttgaacagt cgattctcta tcatgtagag
     9121 gtttataaag ccttgctgga tttgtgtctc ttgcttccag acttgcaaga tagttaccga
     9181 gagttgctgg aaaagatggc gacctatatt caaatgatga caggcttaga tggacgaact
     9241 ttggcttttg gtgatagcga ttctacagaa acgacagaaa ttttgagcct gtctgctgtg
     9301 gttttgaaca aggaagacct tcttaacggt ctggatgtta aagtcgattt gcttagcctc
     9361 ttgttcctgg ggcgagaaaa ggtcaagcga ctgcaggaat ttgaaaggag agcttggcag
     9421 cctaagtcca tgatctttga agactctgga catgtctgca ttaaggatga acatcgttat
     9481 ctatttttca aaaatggtcc gctaggaagt gcccatagcc atagtgacga gaatagtttt
     9541 tgcttacagt atcaaggcca acctattttc atagatgctg ggcgttattc ttatcgggag
     9601 atatatgaac gttatctctt aaagagtgct tggagtcatt cgacctgcat tgtagatggg
     9661 aaagctccgg aaagaatcac gggatcctgg gaatatgaat actatcctca ctccctgttt
     9721 tgtcaccata aagaaaggga gggagtgcat tatattgagg gggcttattg gtcagcagaa
     9781 cctgatttgc cttatcttca caagagaaaa atcctcatgt tggtagagga tgtctggctc
     9841 ttggtagatg acatcaggtg tcaaggtcag catgaggcgt tgactcagtt tatccttgac
     9901 aaggatgtga cctatcaaga tgggaaaatc aatcagttga gactatggag tgaagttgat
     9961 tttgatttgg aagataccat catttctcct aaatacaatg agcttgaaag aagtagcaaa
    10021 ctcaccaagc gccaattctt tgagaatcag atgctggatt ataccatcat tgcgcatgag
    10081 agttttgaaa ttatccgtca ttctgtctac cagacagatg atcgtgaagt ggaaaatgct
    10141 ctggcttttg aagtgaaaaa tgacgaaaca gacaagctga ttctgttatt aagcgaggat
    10201 attggtgtag gtgaaaaatt gtgcctcgtt gacggaacaa aaatgcgtgg aaaatgtcta
    10261 gtatatgata aaataaatga gagaatgatt cgcttgcagt gctagaaata ggcattttga
    10321 atagtgaata tgttataata agtattagta ggaggtgttt tagattggag aagaaactga
    10381 ccataaaaga cattgcggaa atggctcaga cctcgaaaac aaccgtgtca ttttacctaa
    10441 acgggaaata tgaaaaaatg tcccaagaga cacgtgaaaa gattgaaaaa gttattcatg
    10501 aaacaaatta caaaccgagc attgttgcgc gtagcttaaa ctccaaacga acaaaattaa
    10561 tcggtgtttt gatcggtgat attaccaaca gtttctcaaa ccaaattgtt aagggaattg
    10621 aggatatcgc cagccagaat ggctaccagg taatgatagg aaatagtaat tacagccaag
    10681 agagtgagga ccggtatatt gaaagcatgc ttctcttggg agtagacggc tttattattc
    10741 agccgacctc taatttccga aaatattctc gtatcatcga tgagaaaaag aagaaaatgg
    10801 tcttttttga tagtcagctc tatgaacacc ggactagctg ggttaaaacc aataactatg
    10861 atgccgttta tgacatgacc cagtcctgta tcgaaaaagg ttatgaacat tttctcttga
    10921 ttacagcgga tacgagtcgt ttgagtactc ggattgagcg ggcaagtggt tttgtggatg
    10981 ctttaacaga tgctaatatg cgtcacgcca gtctaaccat tgaagataag catacgaatt
    11041 tggaacaaat taaggaattt ttacaaaaag aaatcgatcc cgatgaaaaa actctggtat
    11101 ttatccctaa ctgttgggcc ctacctctag tctttaccgt tatcaaagag ttgaattata
    11161 acttgccaca agttgggttg attggttttg acaatacgga gtggacttgc ttttcttctc
    11221 caagtgtttc gacgctggtt cagccctcct ttgaggaagg acaacaggct acaaagattt
    11281 tgattgatca gattgaaggc cgcaatcaag aagaaaggca acaagtcttg gattgtagtg
    11341 tgaattggaa agagtcgact ttctaaaatg aaggaaaatg acttgcaatt tctgttaaga
    11401 aataaaataa tcccacctag aacaagctag gtgggattat ttgcctatga aatgagaaat
    11461 tatgggagca agctcctaaa tcaactgttt ttgatctact tctttaacta cttgataaaa
    11521 gttatagaag taggccaaac ttgaaatgat ggttacgact aggaatattg aaaatttcca
    11581 ttggacaggg ttggttaaaa gttgtggaaa ggatatgagg agaaagaaga gggctgcgtt
    11641 gaggacaggt atccgttttg attgtatttt ctcaagtcct ttattgagcg caggaagaaa
    11701 gaggagtagg agtagtaaaa ctgtatgaga aacagctcct gaaataaggg cgaagaaaag
    11761 gaaaatactg ataaaaacat gaatgatcag tagtctagct agtgatttca taaggcacct
    11821 cctaatcctg gtctttttta gctcttgcaa tacgaagtga gtcgacaata tgtatcatca
    11881 ctccgaaaaa gaaagctccc agtatagttt taaaaatatg ttttgtattt agaagagaac
    11941 tgataaaatt tggattttca cttgttaggg tatcaatgag tggaattata aaaaatatca
    12001 ctgttccata aatcgaacct gctttcagac caggataacg taactgtttc ttttcttttt
    12061 tcatgagttt gctccttttt taaaaatctt ccttccaaaa gagactgttg aggtcagttt
    12121 ggaggctgcg agcgagattg agacagagtt ccagagttgg attgtacttg tcgttttcaa
    12181 tcatattgat ggtctgtctc gagacaccga tatccttggc gagctcgagc tgggaaatgc
    12241 ccagttcctt gcgaaattct ttcacacgat tcatctggtc tcctttctga tttgtgtcgt
    12301 atatatttga ctttattata gtcttttaaa cataaagtgt caagtatttt tgacatattt
    12361 tttgaagaaa tagtagtctc cttgtcctat ttgtctgaca agtgcaagct ggtcggattt
    12421 gtggtaaaat agataagata tgacaaaaga atttcatcat gtaacggtct tactccacga
    12481 aacgattgat atgcttgacg taaaacctga cggtatctac gttgatgcga ctttgggtgg
    12541 agcaggccat agcgagtatt tattaagtaa attaagtgaa aaaggccatc tctatgcctt
    12601 tgaccaggat cagaatgcca ttgacaatgc gcaaaaacgc ttggcacctt acattgagaa
    12661 gggaatggtg acctttatca aggacaactt ccgtcattta caggcacgtt tgcgcgaagc
    12721 tggtgttcag gaaattgatg gaatttgtta tgacttggga gtgtctagtc ctcaattaga
    12781 ccagcgtgag cgtggttttt cttataaaaa ggatgcgcca ctggacatgc ggatgaatca
    12841 ggatgctagt ctgacagcct atgaagtggt gaacaattat gactatcatg acttggttcg
    12901 tattttcttc aagtatggag aggacaaatt ctctaaacag attgcgcgta agattgagca
    12961 agcgcgtgaa gtgaagccga ttgagacaac gactgagtta gcagagatta tcaagttggt
    13021 caaacctgcc aaggaactca agaagaaggg tcatcctgct aagcagattt tccaggctat
    13081 tcgaattgaa gtcaatgatg aactgggagc ggcagatgag tccatccagc aggctatgga
    13141 tatgttggct ctggatggta gaatttcagt gattaccttt cattccttag aagaccgctt
    13201 gaccaagcaa ttgttcaagg aagcttcaac agttgaagtt ccaaaaggct tgcctttcat
    13261 cccagatgat ctcaagccca agatggaatt ggtgtcccgt aagccaatct tgccaagtgc
    13321 ggaagagtta gaagccaata accgctcgca ctcagccaag ttgcgcgtgg tcagaaaaat
    13381 tcacaagtaa gagggaaaaa gatggcagaa aaaatggaaa aaacaggtca aatactacag
    13441 atgcaactta aacggttttc gcgtgtggaa aaagcttttt acttttccat tgctgtaacc
    13501 actcttattg tagccattag tattattttt atgcagacca agctcttgca agtgcagaat
    13561 gatttgacaa aaatcaatgc gcagatagag gaaaagaaga ccgaattgga cgatgccaag
    13621 caagaggtca atgaactatt acgtgcagaa cgtttgaaag aaattgccaa ttcacacgat
    13681 ttgcaattaa acaatgaaaa tattcgaata gcggagtaag atatgaagtg gacaaaaaga
    13741 gtaatccgtt atgcgacgaa aaatcggaaa tcgccggctg aaaacagacg cagagttgga
    13801 aaaagtctga gtttattatc tgtctttgtt tttgccgttt ttttagtcaa ttttgcagtc
    13861 attattggga cgggcactcg ctttggaaca gatttagcga aggaagctaa gaaggttcat
    13921 caaaccaccc gtacagttcc tgccaaacgt gggactattt atgaccgaaa tggagtcccg
    13981 attgctgagg atgcgacatc ttataatgtc tatgcggtca ttgatgagaa ctataagtca
    14041 gcaacgggta agattcttta cgtagaaaaa acacaattta acaaggttgc agaggtcttt
    14101 cataagtatc tggacatgga agaatcctat gtaatagagc aactctcgca acctaatctc
    14161 aagcaagttt cctttggagc aaagggaaat gggattacct atgccaatat gatgtctatc
    14221 aaaaaagaat tggaagctgc agaggtcaag gggattgatt ttacaaccag tcccaatcgt
    14281 agttacccaa acggacaatt tgcttctagt tttatcggtc tagctcagct ccatgaaaat
    14341 gaagatggaa gcaagagctt gctgggaacc tctggaatgg agagttcctt gaacagtatt
    14401 cttgcaggga cagacggcat tattacctat gaaaaggatc gtctgggtaa tattgtaccc
    14461 ggaacagaac aagtttccca acgaacgatg gacggtaagg atgtttatac aaccatttcc
    14521 agccccctcc agtcctttat ggaaacccag atggatgctt ttcaagagaa ggtaaaagga
    14581 aagtacatga cagcgacttt ggtcagtgct aaaacagggg aaattctggc aacgacacaa
    14641 cgaccgacct ttgatgcaga tacaaaagaa ggcattacag aggactttgt ctggcgtgat
    14701 attctttatc aaagtaacta tgagccaggt tccactatga aagtgatgat gttggctgct
    14761 gctattgata ataatacctt cccaagtgga gaatacttca atagcagtga attaaaaata
    14821 gcggatgcga cgattcgaga ttgggatgtt aatgatggtt tgacgactgg tgggatgatg
    14881 actttcttac aaggtttcgc tcactccagt aatgttggaa tgagtctact tgaacaaaaa
    14941 atgggagatg ctacttggtt ggattatcta aaccgcttta aatttggggt cccaactcgc
    15001 tttggcttga cagatgaata cgctggtcaa cttccagctg acaatattgt caatattgcc
    15061 atgagtgcat ttggtcaggg gatttctgta acgcagaccc agatgctacg tgcttttaca
    15121 gccattgcca acgatggggt tatgttggaa ccaaaattta tcagtgccct ttatgatcca
    15181 aatgatcaaa ctgctcggaa atctcaaaaa gaaattgtgg gaaatcctgt ttctaaagat
    15241 gcagctagtc taactcggac taatatgatt ttggtaggga cggatccggt ttatggaacc
    15301 atgtataacc acagcacagg caagccaact gtaactgttc ctgggcaaaa tgtagccctc
    15361 aagtctggta cggctgaaat agctgacgag aaaaatggag gctacttagt tggttctact
    15421 aacaacattt tctcggttgt agccatgaac cctgctgaaa atcctgactt tatcttgtat
    15481 gtaacggttc aacagcctga acattattca ggtatccagt tgggagaatt tacaaatcct
    15541 atcttggaac gagcttcagc tatgaaagac tctctcaatc ttcaaacaac agctaaagct
    15601 ttggagcaag taagtcaaca aagtccttat cctatgccta gtgtcaagga tatttcacct
    15661 ggtgatttag cagaagaatt gcgtcgcaat cttgtacaac ctatcgttgt gggaacagga
    15721 acgaagatta aaaacagttc tgctgaagaa gggaagaatc ttgccccgaa tcagcaagtc
    15781 ctgatcttat ctgataaagc agaggaagtg ccagacatgt atggttggac aaaagcgact
    15841 gctgagacct ttgctaagtg gctcaatata gaacttgaat ttgaaggttc gggctctact
    15901 gtgcagaagc aagatgttcg tgctaacaca gctatcaagg acattaaaaa aattacatta
    15961 actttaggag actaatatgt ttatttccat cagtgctgga attgtgacat ttttactaac
    16021 tttagtagga attccggcct ttatccaatt ttatagaaag gcgcaaatta caggccagca
    16081 gatgcatgag gatgtcaaac agcatcaggc aaaagctggg actcctacaa tgggaggttt
    16141 ggtttttttg atcacttctg ttttggttgc tttctttttc gccctattta gtagccaatt
    16201 cagcaataat gtgggaatga ttttgttcat cttggtcttg tatggcttgg tcggattttt
    16261 agatgacttt ctcaaggtct ttcgtaaaat caatgagggg cttaatccta agcaaaaatt
    16321 agttcttcag cttctaggtg gagttatctt ctatcttttc tatgagcgtg ggggcgatat
    16381 gctttctgtc tttggttatc aagtgcatct agggattttc tatattgtct tcgctctttt
    16441 ctggctagtc ggtttttcaa acgcagtaaa cttgacagac ggtgttgacg gtttagctag
    16501 tatttccgtt gtgattagtt tgtctgccta tggagttatt gcctatgtgc aaggtcagat
    16561 ggatattctt ttagtgattc ttgccatgat tggtggtttg ctcggtttct tcatctttaa
    16621 ccataagcct gccaaggtct ttatgggtga tgtgggaagt ttggccctag gtgggatgct
    16681 ggcagctatc tctatggctc tccaccaaga atggactctc ttgattatcg gaattgtata
    16741 tgtttttgaa acaacttctg ttatgatgca agtcagttat ttcaaattga caggtggtaa
    16801 acgtattttc cgtatgacgc ctgtacatca ccattttgag cttgggggat tgtctggtaa
    16861 aggaaatcct tggagcgagt ggaaggttga cttcttcttt tggggagtgg gacttctagc
    16921 aagtctcctg accctagcaa ttttgtattt aatgtaagaa tggcatcctg atgtttcagg
    16981 gtgtttttgt atttaaatac acaatgaaaa tcaaagaaca aactagaaag ctaactttag
    17041 gctgctcaaa atatagtaga ttgaaactag aatagtacac atctacttct aaaatattgt
    17101 tagaaatcga tttgactgtc ctgatcgatt tgtcctgttc ttatttcatt ttaatatata
    17161 gttttgagat ggttgataga actgacgaag tcagctcaaa acatggtttt gaggttgtag
    17221 ataaggagaa gctgatgtgg tttgaagagg ttttcgaaga gtgtaaaaaa atattggtca
    17281 gttaaagcca aaactcttga ccttttctga ccaatgtagt atattatgaa cgtaaggtaa
    17341 atgaaagcct tactagaaca cttatttaaa gtaaaataga aagaggtggg tctatgtttc
    17401 atctagaaat cttcagaagt aaagatggtc tactcctgct tgaaaaagaa aaaccagaaa
    17461 tagtacgtag ggtagcggtt taactagtct aaatttttta aaacaatggt caaagatagt
    17521 caatatcagt aatcataact aagtaaacaa aaagaggtaa agaatatgaa taacaacttt
    17581 aataatttta ataacatgga tgatttattt aaccaattga tgggtggtat gcgaggatat
    17641 agttctgaaa atcgccgtta cttaattaat ggacgcgaag tcacacctga ggaatttgct
    17701 cactatcgtg cgactggtca attaccagga aatgcagaaa ctgatgtgca aatgccacaa
    17761 caggcatcag gtatgaaaca agacggtgtc cttgcaaaac taggtcgaaa cttgacagca
    17821 gaagcgcgtg agggcaagtt ggatcctgtt atcggacgaa acaaggaaat tcaagaaaca
    17881 tctgaaatcc tctcacgccg caccaagaac aatcctgttt tggtcggaga tgcaggtgtt
    17941 ggtaagacag cagttgtcga aggtctagcg caagccattg tgaacggaga tgttcctgct
    18001 gctatcaaga acaaggaaat tatttctatt gatatctcag gtcttgaggc tggtactcaa
    18061 taccgtggta gctttgaaga aaatgtccaa aacttagtca atgaagtgaa agaagcaggg
    18121 aatattatcc tcttctttga tgaaattcac caaattcttg gtgctggtag cactggtgga
    18181 gacagtggtt ctaaagggct tgcggatatt ctcaagccag ctctctctcg tggagaattg
    18241 acagtgattg gggcaacaac tcaagacgaa taccgtaaca ccatcttgaa gaatgctgct
    18301 cttgctcgtc gtttcaacga agtgaaggtc aatgctcctt cagcagagaa tacttttaaa
    18361 attcttcaag gcattcgtga cctctatcaa caacaccaca atgtcatctt gccagacgaa
    18421 gtcttgaaag cagcggtgga ttattctgtt caatacattc ctcaacgtag cttgccagat
    18481 aaggctattg accttgtcga tgtaacggct gctcacttgg cggctcaaca tccagtaaca
    18541 gatgtgcatg ctgttgaacg agaaatcgaa acggaaaaag acaagcaaga aaaagcagtt
    18601 gaagcagaag attttgaagc agctctaaac tataaaacac gcattgcaga attggaaagg
    18661 aaaatcgaaa accacacaga agatatgaaa gtgactgcaa gtgtcaacga tgtggctgaa
    18721 tctgtggaac gaatgacagg tatcccagta tcgcaaatgg gagcttcaga tatcgaacgt
    18781 ttgaaagata tggctcatcg cttgcaaaac aaggtgattg gtcaagataa ggccgtagaa
    18841 gctgtagctc gtgctatccg tcgtaaccgt gctggttttg atgaaggaaa tcgcccaatc
    18901 ggcagcttcc tctttgtagg gtctactggg gttggtaaga cggagcttgc taagcaattg
    18961 gcactcgata tgtttggaac ccaggatgcg attatccgtt tggatatgtc tgaatacagt
    19021 gaccgcacag ctgtttctaa gctaattggt acaacagcag gctatgtggg ttatgatgac
    19081 aatagcaata ccttaacaga acgtgttcgt cgcaatccat actctatcat tctcttggat
    19141 gaaattgaaa aggctgaccc tcaagttatt acccttctcc tccaagttct agatgatggt
    19201 cgtttgacag atggtcaagg aaatacagta aacttcaaga acactgtcat tattgcgacc
    19261 tcaaatgctg gatttggcta tgaagccaac ttgacagaag atgcggataa accagaattg
    19321 atggaccgtt tgaacccctt cttccgtcca gaactcctca accgctttaa tgcagtcatc
    19381 gagttctcac acttgactaa ggaagatctt tctaagattg tagatttgat gttggctgaa
    19441 gttaaccaaa ccttggctaa gaaagacatt gacttggtag ttagtcaagc ggctaaagat
    19501 tatatcacag aagaaggtta cgacgaagtc atgggggttc gtcctctccg tcgcgtggtt
    19561 gaacaagaaa ttcgtgataa ggtgacagac ttccacttgg atcatttaga tgctaaacat
    19621 ctggaagcag atatggaaga tggcgttttg gttattcgtg agaaagccta agatagaatt
    19681 ttgaggataa aaaagaagga gccagctgaa aaaaactggt tccttttgag gtacgacggg
    19741 catgtcgtat agtaggttga aactagaata gtacacatct acttctaaaa cattgttaga
    19801 aatcgatttg actgtcctga acgatttgtc ctgttcttat ttcattttac tatatatctg
    19861 aatatctgag gtgtaagtcc tcggtgggca cccactaccg gtgaacccaa tagcgattcc
    19921 caagcctgac tatcgtgagg tagtggatta aaacggtctg gggatagacc gttttaagtc
    19981 tgacgctgga aataagaatc gtcagaggaa gggatagcga aatcgtggct ttacgaacag
    20041 gaacgtgata gtaaggcgta tatagcggat aagggggtct caaactctaa agttcaaaaa
    20101 ggtagtcgta acctatatgc gtaaatcacg agagcaactg aatttgttca ttcgaggatg
    20161 gataaactat ttctcattgg gaaatatgaa aagtatagtc gccagcatag atgagcgctt
    20221 gcgtactcgc ctacgagtga ttatctggaa gcaatggaag aagaaatcga gacgattatg
    20281 gggattgctt aagttaggag ttcctaaatg gatagcagat aaggtatctg gctggggcga
    20341 ccattatcaa ttagtagctc agaagtcggt acttaaacgt gctatatcaa aaccagtcct
    20401 ggaaaaacgt ggactggttt cgtgtttgga ttattacctt gaacgacatg cgttaaaagt
    20461 tagttgaacc gccgtatgcc ggacggcacg tacggtggtg tgagaggggc tagagattat
    20521 cccctactcg attttaaatc acatgacgtt caaaggcatc atctgaaatc ccttgttcca
    20581 agatgagttt tgcccattct ttagcagaga agaggctgtg gtccttgtag tttccgcaag
    20641 attcgatggt tgtccctggg acatcttccc aagtagtagt ttcagcgatt tccttgagcg
    20701 aatccttgat aacagctgcg attttagcac tggtgtgacg tccccacata atcatgtgga
    20761 agcctgtgcg gcaaccaaat ggtgaacagt caatcatgcc gtcaatgcgg gtacggatga
    20821 gtttggctaa gaggtgctcg atagtgtgaa ggccggcagt agggatagag tcttcgtttg
    20881 gttgcaccaa gcgaatatca taattggaga tgatgtctcc ttttggtcct gtttcttccc
    20941 caatcaagcg aacatagggt gctttgacaa tggtgtggtc aagttcaaaa ctttcgacaa
    21001 taacttcttt tgacatggta aatcctttca gttttcttct ctcattatat cataaaggtt
    21061 gctcctgaga cagagagaaa acctctccga ggctggagag gttgaaatct ttacttacga
    21121 tataagcggt cgtattggta gtatgggtca aaggttacgt tgatacccag tttacgaagg
    21181 acattcttgt cttcatcagt caagatgatg gttgagtggg cttcgcttcc tttgaggttg
    21241 ccgagttctt ccatagcgcg ggcagcatca ggattttctg tagctgtgat agcaagtgca
    21301 atcaggattt catttgaatg aaggcgtgga ttgcggctac cgagatgatc gattttaaga
    21361 ccttggattg gcttaacaac ttccggctcg attaatttta cttctttagc gatatcagct
    21421 gattttttga tggcgttgat caaggcagcg gctgtagggc caaagagttc tgagttctta
    21481 ccagtgacga tttccccatt tggcaattca aaggctaggg ctggtccacc agtttcttct
    21541 gctttttggc gcgcaacgac agcaacctta cggtctgcag gtgtgatacc gaggtcgttc
    21601 atgagcaact caattttctt gacggcagct tcgccaactt tttcagcttt gaagtcaaga
    21661 actgtttgat agtaacggcg gatgatttct tgtttagaag cttcgacagc agcctcgtca
    21721 tctgtaatag cgaaaccaac catgttgaca cccatatctg tcggtgaagc gtatggtgat
    21781 tttccgagaa tacgttccaa catgcgcttg agcactggga agatttcgat atcacggttg
    21841 tagttgacag tggtttctcc ataggtttga agatggaagg ggtcaatcat gttgacatca
    21901 tcaaggtcag ctgtggcagc ttcataagcc aagttaactg gatgatgaag gggaagattc
    21961 caaacaggga aggtttcaaa tttagcgtag ccagatttga tgccattgat ttggtcgtgg
    22021 tacatattgg acatacacgt tgccaatttt ccagaaccgg gtccaggagc ggttacgaca
    22081 atcaagttgc gactggtttt gatgtagtcg tttttgccca tgccttctgg ggaaatgatg
    22141 tgatccatat ccgtcggata tcctttgatt ggataatgaa gataagaatc aattccgttt
    22201 ttctcaagtt gattgcggaa ggcatctgca gcgggttggc cagcgtattg tgtaatgaca
    22261 acggaaccaa caaaaatccc taattcattg aatttgtcaa tcaaacgaag aacttcttgg
    22321 tcataagaaa tgcctaagtc gccacgtgct ttggaatgtt caatgttgct agcattaatg
    22381 gcaatcacaa cctcaacctg ctctttcaat tcttgcaaga gcttgatttt gttgtcaggt
    22441 tcataaccag gaaggacacg agcagcgtgg aaatcttcta acattttacc gccaaactct
    22501 aagtagagct tgccgtcaaa ttggttaatg cgctccaaaa tatggtcgcg ttgtaaattc
    22561 aaatattgtt cagaactaaa agcttgtttt ttcatttttt tacctctgga ctctattata
    22621 ataaaaaatt ggaagttagg aaactacgga gctaaaaaag aaattaaaaa gattaagcaa
    22681 acgcttgcac aaaattttaa aaagtgctat catatactat agattattaa aataatgagg
    22741 taagaagatg caagaaaaat ggtggcataa tgccgtagtc tatcaagtct atccaaagag
    22801 ttttatggat agtaatggag atggagttgg tgatttgcca ggtattacca gtaagttgga
    22861 ctatctagct aagctaggaa tcacagcgat ttggctttct cccgtttatg acagccctat
    22921 ggatgataat ggctacgata ttgctgatta tcaagcgatt gcaacgattt ttggaaccat
    22981 ggaggatatg gatcaactga ttgcggaagc taagaagcgt gacattcgta tcatcatgga
    23041 cttggtggtt aatcatacct cagatgaaca tgcttggttt gtcgaagcct gtgaaaatac
    23101 tgacagccct gagcgagact actatatctg gcgcgatgaa cccaatgacc tagattctat
    23161 ctttagtggg tctgcttggg aatacgatga aaagtcaggt caatactatc tccacttttt
    23221 cagcaaaaaa cagccggatc tcaactggga aaatgaaaaa cttcgccaga aaatttatga
    23281 gatgatgaac ttctggattg ataaaggtat tggtggtttc cgtatggatg ttattgacat
    23341 gattggcaaa attcctgacg agaaggtagt caataatggt cctatgctcc atccctatct
    23401 caaggaaatg aatcaggcga cctttggaga taaggatctc ttgacagtag gggagacttg
    23461 gggagcaacg ccagagattg ccaagctcta ctctgatcca aaggggcaag aattgtctat
    23521 ggtcttccag tttgaacata tcggtcttca gtatcaggaa ggtcagccta aatggcacta
    23581 tcaaaaagag ctgaatatcg ctaagttaaa agaaatcttc aacaaatggc agacagagtt
    23641 aggagttgag gacggctgga attccctctt ctggaacaac catgacctcc ctcgtattgt
    23701 ctcaatctgg ggaaatgacc aagaataccg cgaaaaatct gccaaagcct ttgcaatctt
    23761 acttcatctc atgagaggaa ctccttatat ctaccaaggt gaggagattg ggatgaccaa
    23821 ctatccgttt gaaacactag atcaagtaga agatattgaa tctctcaact atgcgcgtga
    23881 ggctcttgaa aaaggtgttc cgatgcaaga aatcatggac agtatccgtg ttattggacg
    23941 tgacaatgcc cgtaccccta tgcaatggga cgagagcaaa aacgctggtt tctcaacagg
    24001 tcaaccttgg ttggcagtta atccaaatta cgagatgatc aacgtccaag aagcgctggc
    24061 aaatccagat tctattttct atacctatca gaaactggtc caaattcgca aggagaatag
    24121 ctggctaatt cgagctgact ttgaattgct tgatacggct gataaggtct ttgcttatat
    24181 acgtaaggat ggcgaccgtc gcttcctagt tgtggctaac ttgtccaatg aagagcaaga
    24241 cttgacagta gaaggaaaag tcaaatctgt cttgattgaa aacaccctag ctcaagaagt
    24301 ctttgaaaaa caaatcttag ttccatggga tgctttctgt gtggaattac tataaatatt
    24361 ttttgcagaa aaatttaaaa ttgaaatcgt ataaaaacaa gggaggactg tataaaaggc
    24421 agaaatcctt tgttttttat aaccaaggtt tataaacctt cattctcgaa attcaattaa
    24481 ctttacaaat tcccactatt aaggagaaag aagacgaaca taaagaagcg tatccttagt
    24541 gcaggcctga cttttacatc tgctttgctt ttagctgctt gcgaccaatc aggttcagat
    24601 acaaaaactt actcatcaac ctttagtgga aatccaacta catttaacta tctattagac
    24661 tattacgctg ataatatagt caattgaaac aagaacaaga caaaagagcc tcataaaagg
    24721 tattgcaact tggtaatacc tttttgaggt gctttttgat atgagcccat gttttctcaa
    24781 taggattgta ctcaggtgag tagggaggaa gaggtaaaag tttatgccca aactcttcgc
    24841 ataaaagttc tagcttcccc attctatgga atcttgcatt atccataata ataaccgatg
    24901 gtgtggttaa tgttggtaag aaaaacttct gaaaccatac ttcaaaaaag tcgctcgtca
    24961 tcatctcttc gtaagtcatt ggagcgatta attcaccatt tgttagacct gcaaccaaag
    25021 aaatcctctg atatcttctt ccagatactt tgcctcttct taactgacct tttaatgagc
    25081 gaccatattc tcgataaaaa taagtatcga atcctgtttc atcaatctaa acaggtgcta
    25141 ggtgctttaa actattaaaa ttcttaagaa ataaggctac ttttctgggt cttgttcata
    25201 attagattga aactagagta gtagacctct gcttctaaaa cattgttaga aatcgatttg
    25261 actgtcctga tcgatttgtc atgttcttat ttcattttac tatatttttg tttcgcggga
    25321 agtctactaa gatacttaaa gatgcagata gtaaaaaaag atgcagatag taaaaaaatg
    25381 tagacattac cgtaaaaaag tgatataatt ataagatgtt caatgtatag gtgttaatca
    25441 tgagtagacg ttttaaaaaa tcaggttcac agaaagtgaa gcgaagtgtt aatatagttt
    25501 tgctgactat ttatttattg ttagtttgtt ttttattgtt cttaatcttt aagtacaata
    25561 tccttgcttt tagatatctt aatctagtgg taactgcgtt agtcttacta gtagccttgg
    25621 tagggctact cttgattatc tataaaaaag ctgaaaaatt tactattttt ctgttggtgt
    25681 tctctatcct tgtcagctca gtgtcgctct ttgcagtaca gcagtttgtt ggactgacca
    25741 atcgtttaaa tgcgacttct aattactcag aatattcgct cagtgtcgct gttttagcag
    25801 atagtgagat cgaaaatgtt acgcaactga cgagtgtgac agcaccgact gggactgata
    25861 atgaaaatat tcaaaaacta ctagctgata tcaagttaag tcagaatacc gatttgacag
    25921 tcgaccagag ttcgtcttac ttggttgctt acaagagttt gattgcaggg gagactaagg
    25981 ccattgtcct aaatagtgtc tttgaaaata tcatcgagtc agagtatcca gactacgcat
    26041 cgaagataaa gaagatttat accaagggat ttactaaaaa agtagaagct cctaagacgt
    26101 ctaagaatca gtctttcaat atctatgtta gtggaattga cacctatggt cctattagtt
    26161 cggtgtcgcg atcagatgtc aatatcctga tgactgtcaa tcgtgatacc aagaaaatcc
    26221 tcttgaccac aacgccacgt gatgcctatg taccaatcgc agatggtgga aataatcaaa
    26281 aagataaatt aacccatgcg ggcatttatg gagttgattc gtccattcac accttagaaa
    26341 atctctatgg agtggatatc aattactatg tgcgattgaa cttcacttct ttcttgaaaa
    26401 tgattgactt attgggaggg gtagatgttc ataatgatca agagttttca gctctacatg
    26461 ggaagttcca tttcccagta gggaatgtcc acctagactc tgagcaggct ctaagttttg
    26521 tacgtgaacg ctactcacta gccgatggag accgtgaccg tggtcgcaac caacaaaagg
    26581 tgattgtggc tatccttcaa aaattaacgt caaccgaagc actgaaaaat tatagtacga
    26641 tcattgatag cttgcaagat tctatccaaa caaatatgcc acttgagacc atgatagatt
    26701 tggtcaatgc tcagttagaa agtggtggaa cttacaaagt gaattcgcaa gacttgaaag
    26761 gtacaggtcg gatggatctt ccttcttatg caatgccaga cagtaacctc tatgtgatgg
    26821 aaatagatga tagtagttta gctgtagtta aagcagctat acaggatgtg atggagggta
    26881 gatgaaatga tagacatcca ttcgcatatc gtttttgatg tagatgacgg tcccaagtca
    26941 agagaggaaa gcaaggctct cttggcagaa tcctacaggc agggggtgcg aaccattgtc
    27001 tctacctctc accgtcgcaa gggcatgttt gaaactccgg aagagaagat agcagaaaac
    27061 tttcttcagg ttcgggaaat agctaaggaa gtggcgagtg acttagtcat tgcttacggg
    27121 gctgaaattt actacacacc agatgttctg gataagctgg gaaaaaagcg gattccgacc
    27181 ctcaatgata gtcgttatgc cttgatagag tttagtatga acactcttta tcgcgatatt
    27241 catagcgcct tgaacaagat cttgatgttg ggaattactc cagtcattgc ccacatcgaa
    27301 cgctatgatg ctcttgaaaa taatgaaaaa cgcgttcggg agctgatcga tatgggctgt
    27361 tacacgcaag taaatagttc acatgtcctc aaacccaaac tttttggcga acgttataaa
    27421 ttcatgaaaa aaagagttca gtattttttg gaacgtgatt tagttcatgt agttgcaagt
    27481 gatatgcaca atttagacag tagacctcca tatatgcaac aggcatatga tatcattgct
    27541 aagaaatatg gagcgaaaaa agcgaaagaa ctttttgtag ataatcccag aaaaattata
    27601 atggatcaat taatttagga gaaaatatgc aagatcaaaa cactttggaa atcgatgtat
    27661 ttcaactatt cagagcttta tggaaaagaa agttggtcat tttattagtg gcaattataa
    27721 cttcttcagt tgcttttgcc tacagtactt ttgttatcaa acctgagttt actagtacga
    27781 cccggattta tgtagttaac cgtaatcagg aagagaaatc tggtttaacc aatcaagact
    27841 tgcaggcagg ttcatacttg gttaaagact atcgtgaaat tatcctatcg caggatgttt
    27901 tggaggaagt tgtttctgat ttgaaactag atttgacgcc aaaaggtttg gctaataaaa
    27961 ttaaagtaac agtaccagtt gatacccgta ttgtatctgt ttcagttaat gatcgagttc
    28021 ctgaagaggc aagccgtatc gctaactctt tgagagaagt agctgctcaa aaaattatca
    28081 gtattactcg tgtttctgat gtgacaacac tggaggaggc aagaccggcg acatcaccgt
    28141 cttcgccaaa tattaaacgc aatacactaa ttggtttttt ggcagggggg attggaacta
    28201 gtgttatagt tcttcttctt gaacttttgg acactcatgt gaaacgtccg gaagatatcg
    28261 aagatacact gcagatgaca cttttgggag ttgtaccaaa cttgagtaag ttgaaatagg
    28321 agagaggaat gccgacatta gaaatagcac aaaaaaaact ggagttcatt aagaaggcag
    28381 aagaatatta caatgccttg tgtacaaata tacagttgag cggagataaa ctaaaagtaa
    28441 tttccgttac ttctgttaac cctggggaag gaaaaacaac tacttccgta aatatagcaa
    28501 ggtcgtttgc gcgtgcaggc tataaaactc ttttgatcga tggcgatact cgaaattcag
    28561 ttatatcagg agtttttaaa tcgcgtgaaa aaattacagg gctaacagaa tttttatctg
    28621 ggacagctga tttatctcac ggtttatgtg atacaaatat tgaaaattta tttgtaattc
    28681 aatcgggatc tgtatcacca aaccctacgg ccttgttaca aagtaaaaat tttaatgata
    28741 tgattgaaac attgcgtaaa tattttgatt atatcattgt tgatacacca cctattggaa
    28801 ttgttattga tgcggcaatt atcactcaaa agtgtgatgc gtccatcttg gtaacagcaa
    28861 caggtgaggc gaataaacgt gatgtccaaa aagtgaaaca acaattagaa caaacaggga
    28921 aactgttcct gggagttgtt ttaaataaat tggatatctc ggttgataag tatggagttt
    28981 acggttccta tggaaattat ggtaaaaaat aacttagaaa agattttatg gatgaaaaag
    29041 gattgaa
//
LOCUS       DAYYQN010000023        29033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYYQN010000023 DAYYQN010000000
VERSION     DAYYQN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.023"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1274..1675
                     /locus_tag="ACHBRQ_000948"
                     /pseudo
     CDS             1274..1675
                     /locus_tag="ACHBRQ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
     gene            complement(1800..3239)
                     /locus_tag="ACHBRQ_000949"
     CDS             complement(1800..3239)
                     /locus_tag="ACHBRQ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9592641.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(3243..4592)
                     /gene="trkA"
                     /locus_tag="ACHBRQ_000950"
     CDS             complement(3243..4592)
                     /gene="trkA"
                     /locus_tag="ACHBRQ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFM9592642.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            4781..5629
                     /locus_tag="ACHBRQ_000951"
     CDS             4781..5629
                     /locus_tag="ACHBRQ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFM9592643.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            5644..6192
                     /locus_tag="ACHBRQ_000952"
     CDS             5644..6192
                     /locus_tag="ACHBRQ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HFM9592644.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            6199..6456
                     /locus_tag="ACHBRQ_000953"
     CDS             6199..6456
                     /locus_tag="ACHBRQ_000953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592645.1"
                     /translation="MKIKEQTRKLAAGFSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            6558..8240
                     /locus_tag="ACHBRQ_000954"
     CDS             6558..8240
                     /locus_tag="ACHBRQ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592646.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            8225..9055
                     /locus_tag="ACHBRQ_000955"
     CDS             8225..9055
                     /locus_tag="ACHBRQ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9592647.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            9248..9751
                     /locus_tag="ACHBRQ_000956"
     CDS             9248..9751
                     /locus_tag="ACHBRQ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HFM9592648.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      9805..10039
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            10204..10767
                     /locus_tag="ACHBRQ_000957"
     CDS             10204..10767
                     /locus_tag="ACHBRQ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9592649.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            10757..11407
                     /locus_tag="ACHBRQ_000958"
     CDS             10757..11407
                     /locus_tag="ACHBRQ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9592650.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIR
                     GDLPDYLTILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     IFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            11435..11824
                     /locus_tag="ACHBRQ_000959"
     CDS             11435..11824
                     /locus_tag="ACHBRQ_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFM9592651.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            11829..12278
                     /locus_tag="ACHBRQ_000960"
     CDS             11829..12278
                     /locus_tag="ACHBRQ_000960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592652.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            12406..12993
                     /gene="rpoE"
                     /locus_tag="ACHBRQ_000961"
     CDS             12406..12993
                     /gene="rpoE"
                     /locus_tag="ACHBRQ_000961"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9592653.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13129..13254
                     /locus_tag="ACHBRQ_000962"
     CDS             13129..13254
                     /locus_tag="ACHBRQ_000962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592654.1"
                     /translation="MKIKEQARKLAVGCSKHCFEVVDKTDKVRNTYLRQGDVDAV"
     gene            13383..14990
                     /locus_tag="ACHBRQ_000963"
     CDS             13383..14990
                     /locus_tag="ACHBRQ_000963"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9592655.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPVADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYANDAEVKINWINAND
                     VTAENVVELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDVEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(15550..17181)
                     /locus_tag="ACHBRQ_000964"
     CDS             complement(15550..17181)
                     /locus_tag="ACHBRQ_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFM9592656.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            17474..22384
                     /locus_tag="ACHBRQ_000965"
     CDS             17474..22384
                     /locus_tag="ACHBRQ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9592657.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTSAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQKPKKNYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKIGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHEISTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLDAKPNHPSGSALAPLLEKNK
                     VLVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            22474..23670
                     /gene="pgk"
                     /locus_tag="ACHBRQ_000966"
     CDS             22474..23670
                     /gene="pgk"
                     /locus_tag="ACHBRQ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9592658.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            23806..24333
                     /locus_tag="ACHBRQ_000967"
     CDS             23806..24333
                     /locus_tag="ACHBRQ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9592659.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            24410..24766
                     /gene="glnR"
                     /locus_tag="ACHBRQ_000968"
     CDS             24410..24766
                     /gene="glnR"
                     /locus_tag="ACHBRQ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HFM9592660.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            24803..26149
                     /gene="glnA"
                     /locus_tag="ACHBRQ_000969"
     CDS             24803..26149
                     /gene="glnA"
                     /locus_tag="ACHBRQ_000969"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9592661.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(26245..26364)
                     /locus_tag="ACHBRQ_000970"
     CDS             complement(26245..26364)
                     /locus_tag="ACHBRQ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HFM9592662.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            26329..26478
                     /locus_tag="ACHBRQ_000971"
     CDS             26329..26478
                     /locus_tag="ACHBRQ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HFM9592663.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            26622..26759
                     /locus_tag="ACHBRQ_000972"
     CDS             26622..26759
                     /locus_tag="ACHBRQ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592664.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            26945..28228
                     /locus_tag="ACHBRQ_000973"
     CDS             26945..28228
                     /locus_tag="ACHBRQ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9592665.1"
                     /translation="MVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKE
                     KIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLF
                     YILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDE
                     YAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFE
                     EGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIP
                     IYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIY
                     HENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELL
                     TKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            28285..>29033
                     /gene="psrA"
                     /locus_tag="ACHBRQ_000974"
     CDS             28285..>29033
                     /gene="psrA"
                     /locus_tag="ACHBRQ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HFM9592666.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLR"
BASE COUNT         8996 a         5179 c         6330 g         8528 t
ORIGIN      
        1 gaaaaattct gtttttcatc ttagaaaaga atgtttagaa atgtaaaatg aatctttttt
       61 cttatacgaa aaccttacta aagtatataa tttaggaagt caattctgta tgataatgtt
      121 tgtactatcg tcgagttttt gcttgattgc ctaacgagat aaattttaga agaattttcg
      181 gtgtgatttg acaagcttct gactggaagg atagttacac tagtattgtt tattaaaatg
      241 agcagataga gaaaatttgg aatctatttg cttattatca atcgtttttt tagcttattt
      301 gtcttagatt ggtatagaac tgatctatgg attaatcctc ttgtatctta tcctgttcct
      361 aaatatgttg gtagaaaaat gaagcagttg ttacgagtgt ctgtattggg aaaaattttg
      421 tctatagaat tctttaaatg atatgaaggc taggaatgcc aaaacactga catatttcgt
      481 ttaaagtcta tccagtctct tcaattgtat ttgtagaaat gctatcagta atatattcgc
      541 gaacactatg atttacaaca gtgtggtcgt acatctttgt aactttatta tacaacaacc
      601 atttacaggt gtaaagttga gcagtgtctt tagtaaattt agggacttgt tttgtcatag
      661 ttttgatacc tgtattcgtt gtttcatgag catttgacgt ttcttttcat gtaggcattt
      721 caacaattag aatgatattt ttccgaggat gaacttatat attgtgactt tttagagtta
      781 tgatgcctct tgatttgact atcgctatca atttcatcca cttttacagt ttcatggttt
      841 tatgaaccga aacatctagg tacaggtttt agcatcaatt gattgttttt gagccatatg
      901 aagttgaaac gtggagatgc ttttcttgtg ataacgccct attcaaagag gaataaccgt
      961 ttttggagag agttcagtac tatgtggcgc taaatactat atgtaaaaca tatattaaac
     1021 aagatttaac taaggagaat tgtaaaaaca aaaatgagaa tttcatataa agtttattct
     1081 aggagtatgt atcttgaata aaaatcgtta tttaagttca gggttatgat gaatgttttt
     1141 ataataataa aatgcagcta gaagaaaagt tattttaata taaatcactc aacatagaga
     1201 gaaggcggtt tcctatatgg aagtcgtctt gttttcatat tgtctaaata cgagatatct
     1261 actacaggtt gtagatattg caagctcaaa tgatattatt ttagaggagg atacaagtga
     1321 aataatgcct ggtgagatag atgtaataaa aagtaaggag ttgaaagtac tggttctttg
     1381 tgcagggtct ggaacaagtg cgtaattagc caatgcaatt aacgagggcg ctcaattagc
     1441 agaagttaga gtgattgcga attcaggagc gtacggagct cattatgata ttatgggtgt
     1501 ttatgattta attattctgg ctccacaagt tcggagttat tatagagaga tgaaggtgga
     1561 tgcagaaaga ttaggtattc agatagttgc taccagagga atggaatata ttcatttaac
     1621 aaagaatcca agtaaagcct tacaatttgt attggagcat taccaagctg tgtagtaagt
     1681 ttttccatct tttatttgag taagaacttt gcttacagac aggcttggat ttaaaaacgt
     1741 tccccctttt taatataaaa atccctcttt cacaattgta aaaagaggga ttttgtattt
     1801 tatctcttag accaagttct tttcataaag agaaggagga ttgggtaaat ctccaagcgc
     1861 cctgcaatca ttgcaaagga gaggagaatt tttgagatgg gactaaagat tgagaaacta
     1921 gaagtggttc ctagaatagg cccgatatta ttgaaacagc taaagacagc gctggtcacg
     1981 accagaaaat cattgctatc taggctgaca ataaagataa gcgctagcaa aatcatagca
     2041 tagatgacaa agtacttgag aatcttatgc tgggtatctt tatcaatcac cgtttgattc
     2101 acatggagag tcaaaacacg gtggggcgat aggattgaca aaatttggtt tttggcaatt
     2161 tttgaaagga tgaggcctcg aataatcttg agtccacctg cagttgatcc agcagagcca
     2221 ccgattgcca tgaggaaaag gaggataaac tgggagaaga ggggccagtt ggtaatatct
     2281 ccatatccaa aaccagttgt tgtaatgatg ttggaaacct ggaagaaggt catttcaaag
     2341 ctctttgaaa accctgggta gaggtagagg gtgttgaggc taatcaagcc tgtagaaacc
     2401 agtacaatga ccaagtaagc ccgaagttct tcgtcaccaa agaaggcctt gatgcgacgg
     2461 agcatgaggt agtagtagag gttgaaattt accccaaaaa ccagaactcc gatactgacc
     2521 agataggtaa tcagtgagct gccatagtgg gcaattccgt cgttatagac ggtaaagcct
     2581 ccagttcccg ctgtccccat agcaatgaca aaactatcgt agagaggcat accagctaga
     2641 taatagatga tgacaaagag ggagaagaga gctagataaa ggagatagag aatctgggca
     2701 gtgtttttta gtttggatac aaccttgcca aaaacaggac ctggaacctc agccttcatc
     2761 acctctaggt ggctattttt ggcattgtcc ataatagcaa gtgcaaaaac aagcactccc
     2821 atccctccaa tcaagtgggt aaaacttcgc cagaagagga gggaacggct gagaaccgaa
     2881 acgtcgttca aaatacttgc tccagtagtt gtaaacccag aactaatttc aaaaaaggca
     2941 tcaataaggc tagggatttg cccagaaaag acaaagggga gaccaccaaa gaaagaccaa
     3001 aggatccaac agagggcaac gatcaagact ccctccttgg cataaatccg ttgatttttt
     3061 ggcttctgta aactccctga accgcctaac aatacgagaa tccctatggt cgaaaagagg
     3121 gctgtaaaga cttggctcga ttcacggtaa tagacagcaa tcgcaacagg aaccaaaaga
     3181 agaacagctt caatcaaaag taattttgaa aggaggtaac gaatcatact tttattcatt
     3241 tcttacctcg cgatcaagtc ataaatcttg gtgatgtttg gcaacaaggt tgttactagg
     3301 agcttgtctc cgacttccaa catatcctcc ccagtaggga aaatagtctt gccctttcga
     3361 atgatggctg caataagaac accttttttt aatttcagtt gagaaagagg tttggcagtc
     3421 attttattgg cttccttgat atggaattgc agggtttcga tttgaccatt ggctagatgg
     3481 tgcatggcct gaaggtctga atactgggca ttaacacgac cacgaataaa gtgcataatc
     3541 gtgtccacgg caatactttt aggtgtgatg atacttgaaa aatcaggcgc attgataatt
     3601 tcgaggagac tggtacgatt gaccttggta atatttttct gtacacctac cctgtcaagg
     3661 aacatcgaag taatcagatt ttcctcatcg actcctgtta gagtcgcaac ggcatcatag
     3721 tgttgagcac tttcttccag caggatatct tttgcggttc catctccttg aacgatgtag
     3781 agatttggga atttctcgct aaagaagctg gcgatttcag gattgatttc aatgactttt
     3841 gtatcgatac gactatcttt gagaatacca agtagataat aggcaattct acctgcccca
     3901 acgatgagaa ggctcttcac ggcgcgtgat ttaaaataat tatggaagag tatcatatcg
     3961 acacggttac cagtgacaaa gattctatct ttatcctgta cagtcatgtc accgcttgga
     4021 atgataattt gatgatccct ctctatcgca cagacaatga cattaccaaa ttttttacga
     4081 aaatcagaaa tgggcatttg gcaaagaccg ctggtggact tgacgacaaa ttccatgagg
     4141 ctaacgcgtc caccagcaaa gcgttcgaca gacagggcgt tggggaagtc aatgatattc
     4201 gcgatagcgc gggcagccaa gagctcagga ttaacgataa gagaaaaacc gagaatattt
     4261 ttttccttga aataagagtt agaatattca gggttccgca cccgaacgat agtttcttta
     4321 gctcccattt tcttggctag aactgctgca atcatgttga cttcatcgtg ctcagtcagg
     4381 gcgataaaga tatcacaatc ttggacgctg gcttgctcaa gaatggcaaa atcggccccg
     4441 ttaccaagga taccaatgat atcaaagcga ctgacaatat gattgagaac agcttcgtct
     4501 tgctcaatca gcaaaacatc atgcttttct gcaaccaagg agcgacagag ggcaaaacca
     4561 acttttcccc ctccgacaag gataattttc ataataaaac ctactttttc atgatgtaac
     4621 tatcataccc tttttcaaga aaaaatgcac ctactagcta ataacaagaa cttttagtag
     4681 gaaatttgct ataaggtaaa actataccct aaccaattga aatagctatt agcgactttc
     4741 tctgaaatat ggtatgataa aggatataca aggagataaa atgaataata atttactggt
     4801 attacaatca gactttggtt tggttgatgg tgcggtatcg gctatgattg gagtggcttt
     4861 agaagagtct ccaaccttaa aaatccatca cttgacgcac gatatcacgc cttataatat
     4921 tttcgagggg agctatcgtc tctttcagac ggtggattac tggcctgagg gaacgacgtt
     4981 tgtatcggtt gtcgatccag gtgtcggttc gaaacgtaag agtgtagttg ccaagactgc
     5041 aaaaaatcaa tacattgtca cgccagataa tgggacgctt tcctttatca agaaacacgt
     5101 tggcattgta gccattcgtg agatttctga ggtggccaat aggcgtcaaa acacagagca
     5161 ttcttatacc ttccacggtc gtgatgtcta tgcctatact ggtgctaaac tggccagtgg
     5221 tcacattact tttgaggaag tagggccaga gctcagtgtg gaacagattg tagagcttcc
     5281 agtcgtagcg accatcatag aagatcatct ggtgaaggga gccattgata ttctggatgt
     5341 gcgtttcggt tcgctttgga cctctatcac acgggaagaa ttttacaagc tggaaccaga
     5401 atttggtgat cgttttgaag tgaccatcta tcatgctgat atgctggtct atcaaaatca
     5461 ggttgtctat ggcaaatcat ttgcagatgt gagaattggg caacccatcc tttacatcaa
     5521 ctctctctat cgtttaggtt tggctatcaa ccaaggttcc tttgccaagg cctataatgt
     5581 aggtgtcggt tcatcttgga ccattgaaat aaagaaaata gaaggataaa ataggagaat
     5641 agtatggaaa tcaaatttac aattaaacaa gttgttgctg tcggaattgg cgctgccctc
     5701 tttgtcgtca tcgggatgat caacattcca acccctgttc caaatacaag catccagctt
     5761 cagtatgcgg tacaagcgct actttctatt atttttggac cgattatcgg tttgcttgtc
     5821 gggttgattg gtcatgcaat caaggactct cttgctggtt atggtctatg gtggacttgg
     5881 attatcgcta gcggactctt tggtttagtt gtgggacttt ttagaaagta cgttcgagtg
     5941 atcaatggtg tttttgactg gaaagatatt cttattttta acctcattca actacttgca
     6001 aatgcccttg tttggggtgt cttggcacca cttggagatg ttgtgattta tcaagaagcg
     6061 gcagaaaaag tatttgcaca agggattgtt gcgggaattg ccaatggtgt atctgtagct
     6121 attgcaggaa ctcttctctt acttgcctat gcaggaactc aaactcgtgc aggaagtttg
     6181 aaaaaggact aatactcaat gaaaatcaaa gagcaaacta ggaagttagc cgcaggtttc
     6241 tcaaaacagt gttttgagat tgtggataga actgacgaag tcagctcaaa acatggtttt
     6301 gaggttgtag atgaaactga cgaagtcagt aaccatacct acggcaaggc gaagctgacg
     6361 tggtttgaag agattttcga agagtataaa atgatgggga aggctgggca actagtcttt
     6421 ttcgatgttt atagattagt taggcaagta agctaagata ggaatctttt tggcgctaag
     6481 attttaagag aagagactgc aagaagggct tgatttatga taaaatagaa gtctaagaag
     6541 cgaatgaaga gagtaagatg aaagaagcta taattgagtg gaaggatttc tctttccggt
     6601 atgaaacaca acaagaaccg accttgcaag ggatagactt gaccatttac aagggagaaa
     6661 aagtcttaat tgttggacca tctggatcag gtaaatctac cttgggtcag tgtttgaatg
     6721 ggattattcc caatatttac aagggtcaga catatggaga atttttgata aagggtcaaa
     6781 cagcctttga tatgagcatc tatgataagt ctcatctggt tagcacagtt ttgcaggata
     6841 cagatgggca gtttattggc ttgtctgtgg cagaagattt ggcgtttgct ctggaaaatg
     6901 atgtgacagc cctagatgag atgaaaggtc gtgtttataa atgggctgaa aagctggacc
     6961 ttcttccttt actggatcag cgtcctcagg atttgtcagg tggacaaaag cagcgagtca
     7021 gtctggctgg tgtcttgatt gatgaaagtc cgattctctt gtttgatgag ccactcgcca
     7081 atctagatcc caagtcaggt caggatatta tcgaattgat tgaccagatt cataaggaag
     7141 aggggacgac gactctgatt atcgagcacc gtttggagga cgttctgcat cgccctgtgg
     7201 atcggattgt cttgataaac gatggtcgta tcctttttaa tgggagccct gaccagttgc
     7261 ttgcgactga tttattgact caaaatggaa ttcgagaacc cctttatcta acgactctcc
     7321 gtcaattagg tgtggactta gtcaaggaag aacaattagc agatctggat aacttgtcta
     7381 tctcaaaagg gcaagtccag ttacagacgg aactggtaaa agaaacccca gcattgcagt
     7441 cactctttag actagaggaa gtatcttttt cttatgatga tagaccgatt ttaaaatccc
     7501 tacatttaga tattaaaaag ggtgaaaaga ttgctattgt cggaaaaaat ggagcaggga
     7561 aatcaactct agccaaggct ataagtagct ttattcagac ggaaggacgc tatctttggg
     7621 aaaaacagga tataaaaggc gattctgttg cagagcgggc ggaacgagta ggctatgtgc
     7681 tacaaaatcc taatcaaatg atttcaacca atatgatttt tgatgaggtg gctctagggc
     7741 tccgtttgcg aggtgtggat gagaaggaaa ttgaaacgag agtctatgaa accttgaaaa
     7801 tctgtggact ttatgaattc cgtaattggc ctatttctgc cctgtcattt ggtcagaaaa
     7861 aacgtgtcac cattgcttca attttggtct taggagctga aattattctc ctagatgaac
     7921 cgactgcagg tcaagatcag aagaactata ctgagattat ggaatttctc gaagagttac
     7981 atcaaaaagg gcataccatt gtcatgatta cccatgatat gcaattgatg ctggattatt
     8041 cagaccgggt ccttgtcatg gtggatggag aattgattgc cgatactgtt ccagccagtc
     8101 tgttgagcga tcctgagctg ttagtaaaag ccaatctaaa agaaacctcc atctttaact
     8161 tggctaagaa actagatgtg gatccactgg atttaacggc attttacaaa gaaaggagag
     8221 agggatgcaa gctaaattaa tcggttacca gcatagagat actgtgattc atcgcttgtc
     8281 aggagctggg aaacttctct ttttcattct ggtatcattg gcggccatga ttagctatga
     8341 caccagaatg ctcttgctga ttgccatctt ttcagtcttt ctcctctatt tatcagaaat
     8401 tcgctttaaa gatgtttcct ttgtagccgt ttttgcgacg gtatttgccg ttttaaacgt
     8461 tttgatggtc tatctctttt ctcccgagta tggggttgga ctttatggag agagaagtgt
     8521 gatttggcag ggaatcggtg tctacactct aaccagccag gagctctttt atctgctaaa
     8581 tttggccatt aagtatcttt gcaccattcc tctggctatt atctttttga tgacaaccca
     8641 tcctagtcag tttgcttcca gtttaaatca aattggtgtg ccctataaga ttgcttattc
     8701 tgtcagcctg accttgcgct atattccaga tttgcaggaa gaattcttta ctatcaagat
     8761 gtctcaggag gcgcgtggga tggaattatc caagaaagct tctcttatgc aacgaatcaa
     8821 aggcaatctg ctcattatta cgcccttgat ttttagctcg ctagaacgca ttgatactat
     8881 tgcgactgcc atggagcttc gccgctttgg gaaagagaaa aaacgcacat ggtatagtta
     8941 tcaggccttg aaaaaaggag actatattac cttgctcttg gcaaccttgt ttttagtagt
     9001 tagtttacta cttatcttgc agaatcaggg acgattttac aacccttgga aatagcttga
     9061 aaaaattgaa aaaatcaagt catttctatt gacaatgatt ctgaaagtgt tatactaaga
     9121 aagtagtttc gctgatttac ttcaaacctg ttgtgaggta agttaacgat gccttaacca
     9181 cgctgtttgc tgagcttgac tccgggcagt gtggctattt ttttgcaatg gtgaaaggaa
     9241 gcaagtcatg acaaatcaca ttgtattatt tgaacctcaa attccacaaa atacaggcaa
     9301 tatcgcgcgt acttgcgctg cgaccaattc tcccctccac atcatcaagc ctatgggctt
     9361 tcctattgat gaccgcaaga tgaagcgggc tggattggat tactgggata agctagagat
     9421 ttatttttac gagagtttgg aagatttcat gtctcagatg aagggaaaac tctatctgat
     9481 ttcgaaattc gcggaaaaag tctattctga ggtggattta tcgactgacg aggaccatta
     9541 ttttctcttt ggacgtgaag acaagggctt acctgaagat tttatgcgag aacatcctga
     9601 gaaggctctc cgtattccca tgaatgatga acatgtccgc agtctcaatg tgtctaatac
     9661 cgtctgcatg attgtctatg aggctcttcg ccagcagaac tttgcaggtc ttgagctcgt
     9721 ccatacctat gaagtggata aattgaaata aaaaatgaaa atgaacaaaa tgcttgcgct
     9781 tgcaagcgtt ttttgttatg ataaaagagt cttcagggca gggtgtgatt cccgaccggc
     9841 ggtgacttta actaggaaat gatcttttcc ttttatactt tgttgacaag ctttgcctaa
     9901 ccagaagtta tgcctacagc ttgtcgccta gtctaaaaga aaaatctcta tttccttctc
     9961 tttaaagaag tccgcgagcg caagctgatg tggtgagatt ccacaaccga cagtatagtc
    10021 tggatgggag aagacgaaag aatagctttg tctgttttga tagatttata gccggattgt
    10081 aaccacttgc tttgtttttc tttaatagaa taagagggaa cttttgggat ataaaaagtg
    10141 agaatagata gaggaatcct ttccaacttc ttctgatttt atagaaaatt ggaggaacct
    10201 gttatgacaa acacacgtcg actttcgacc attgcaattc tatcagccat ctcatttgtg
    10261 ctgatgtact ttgactttcc gcttttacca gcggcatctt tcctcaagat cgaatttagt
    10321 atcttgccag tccttgtggg tctggtggtc atggatttgc ctgctgctct aggagtgctc
    10381 ttgcttcgct cactattgaa attgcttctt aacagccaag gagtgaatac ttacattggt
    10441 ttgccgatga atatcgtagc tttgggagtt tttgtcatcg tatttgcttt gatttggaaa
    10501 aaggaacgga caacccttcg tttcctacta ggctctctag ctggaactgt tggtttaacc
    10561 ttggccatgt tggttctcaa ctatgtttat gctgttcctt tgtacgctaa gtttgctaac
    10621 tttgatattg gaaaaatttt gggactttcc aactacctaa tgaccatggt attacctttt
    10681 aacttgattg agggtgtaat cttttccgtt tcattctggt tgttgtacgt ccttttgaaa
    10741 ccaaccttaa aacactatga aagataaaca aacattttta atgaagggca gttttgccct
    10801 tttacttttc gttattcttg gctatatggt caaattttac cctgaaatgt tggtcaattt
    10861 tgaccaatcg attcagactg ccattcgagg agacttacca gattatttga ctattctttt
    10921 tcgagccctc acacgtctga ttgatatccc agtgattatc acttgggttg tcattacagc
    10981 ttttgtcttt tatcgtaagc gatggaagat agaaagtttc ttcatgctgg gaaatctggc
    11041 tttggcaggt cttttaatcg tgatctttaa aaatatctac cagcgcccac gaccagctat
    11101 cttacatctg gtggaagaga agggattttc cttcccaagt gggcattctc tggctgtaac
    11161 tttgatggta ggtactctga ttgtcattct cagtcaacga attaaaaatc cagtctggag
    11221 aaaaatcgta caaatcgtcc ttggcctcta cctagtcagt gtgctggtat caagagtcta
    11281 tctgggggtt cattacccat cagacgtctt tgccagtctc tgtgtgggct tgggagtcct
    11341 gtttatcgag tttcccttct atgacaagct ccgcttccaa tggcgattta aaggcaaaca
    11401 gaagtgagta tcgaattccc ttgaggagaa agaaatgaaa gtcaatatag cagatcttca
    11461 tccgactcaa ctatatttat cagaaaagaa gttgcaagat attcagatgc tttaccagtc
    11521 ggcagaaaca aaccaagtcg atccaatcag tattcttgcc tttggagatt gtttgctgat
    11581 tacagatggg catcacaggg cttatcaggc tttattggca ggtcgggata cgatttctgc
    11641 tgagtgggat agagatggtg gtgatgaact atatcatctc tatgcgcaag cttgcgagga
    11701 aagaaagatt tactctgttt ttgatttaga agatcgtatc ctagctcaag atgggtatga
    11761 agcaaaatgg tataactggt gtgatggttt taatcaagca gcaactcttt tgttgaaaag
    11821 gtagtgcatt gtgttttgtt ccttattata aggtcaatca ttgtgaggaa gcttttgctt
    11881 ggtatcagga tgtgaacttg gtttacctcg tagatggggt gaagcttcct tacagtcaag
    11941 cgaccttgga agccatgtat tcctatttgg atcggcatgg tgagcttttt tggattgaag
    12001 tcaaggagaa gggagaatgg tttccaattg gggatattac actatctcag gataatctcc
    12061 ccattgtgat tgggaattcc gcttaccaac atcgaggact tggaaaaaag attctaagtg
    12121 ctttgattga attggctcga gtaaaaggat ggaaagaatt gagagtcaag gaaatctaca
    12181 cctacaatca tgcttctagg aggtgtttca agtcgcttgg atttgtggaa aatggagcaa
    12241 cagaaaaagg aaggagtttt atattggaat tagtctaatc ctgccacttt ctccccctta
    12301 acctttgact attccgcaaa attatcgtaa aataaagagt aaatgataaa atgaggtcag
    12361 agtctgttcg ctctgacgat agtagtataa atgaggagaa acgctttgga attagaagta
    12421 tttgctgggc aagaaaaaag tgaactatct atgattgagg tagcgcgtgc tatcttagaa
    12481 cttcgtggtc gcgatcatga gatgcatttt agcgatcttg taaacgaaat tcaaaactac
    12541 cttggaacat caaacagcga tatccgcgaa gctttgcctt tgttctacac agagttgaac
    12601 tttgacggta gcttcatctc acttggggac aacaaatggg gtcttcgttc atggtatggt
    12661 gtggacgaaa tcgacgaaga aatcatcgct cttgaagaaa atgacgacga tgaagtagca
    12721 ccaaaagcta agaaaaaacg tgtcaatgcc tttatggatg gtgattcaga tgccattgac
    12781 tacaatgcag atgatccaga agacgaagat gcatacgaag cagatccagc tctttcatac
    12841 gatgatgaaa atccagatga tgaaaaaaat gaagtggaag cttatgatgc agaaatcaac
    12901 gaaatcgctc cagatgactt gggagaagat gtggatctca acgaagacga cgacgagttt
    12961 tcagatgacg acgctgaaac cagcgaggaa taagagttag ctattgacaa ttatcctatt
    13021 tttaggtatc atattgtttg ggcacctctt ttagaggtcg gggctcccta gttcttaggg
    13081 agctattttt gttttttcaa gaagttatct tcttgtattt tatactcaat gaaaatcaaa
    13141 gagcaagcta ggaaactagc cgtaggctgc tcaaaacact gttttgaggt tgtagataag
    13201 actgacaaag tcaggaacac atatctacgg caaggcgacg ttgacgcggt ttgaagagat
    13261 tttcgaagag tattagttgt gaatctggtg cagtcgtccc agattattct tattagtagg
    13321 gtcttgtttt ctatatcccc tcgtagttaa caagaccttg agcattttag aaagaggaat
    13381 ctatgtctac gaaatatatt tttgtaactg gtggtgtcgt atcgtctatt gggaaaggga
    13441 ttgtggcagc gagtctgggc cgtctcttga aaaatcgtgg tctcaaagta accattcaaa
    13501 agtttgaccc ttatatcaat attgatccgg gaactatgag tccttaccag cacggggaag
    13561 tttttgtgac agatgacgga gctgagacag atttggactt gggtcactat gaacgtttca
    13621 tcgatatcaa tcttaacaaa tattccaacg tgacaactgg taaaatttac agtgaagtgc
    13681 ttcgtaagga acgccgtgga gaataccttg gtgcaactgt tcaggttatt cctcatatca
    13741 cagatgcttt gaaagaaaaa attaagcgtg ccgctctaac gaccgactct gatgtcatta
    13801 tcacagaggt tggtggaaca gtaggagata tcgagtcctt gccattccta gaggcccttc
    13861 gtcagatgaa ggcagatgtg ggggcggata atgttatgta catccataca accttgcttc
    13921 cttacctcaa ggctgctggt gaaatgaaaa ccaaaccaac tcagcattct gtcaaggaat
    13981 tacgcggttt gggaattcaa ccaaatatgt tggttattcg tacagaagag ccagctggtc
    14041 aaggaattaa aaacaaactg gcccagttct gtgatgtggc accagaagcc gttatcgaat
    14101 cgttggatgt cgaacatctt taccaaattc cactgaactt gcaggctcaa ggtatggatc
    14161 aaattgtctg tgaccatttg aaattagacg caccggtagc tgatatgaca gagtggtcag
    14221 caatggtgga caaggtcatg aatctcaaga aacaagttaa aatttccctt gtcggtaagt
    14281 atgttgagtt gcaagatgcc tacatctctg tggttgaagc tttgaaacac tctggttatg
    14341 ccaatgacgc agaagttaaa atcaattgga tcaatgccaa tgatgtgaca gcagagaatg
    14401 tggtagagct tttgtctgat gcggacggaa tcatcgtgcc aggtggtttc ggtcaacgtg
    14461 gtacggaagg gaaaatccaa gctatccgct atgcgcgtga gaatgatgtt ccaatgttgg
    14521 gagtctgctt gggaatgcag ttgacttgta tcgagtttgc tcgtcacgtt ttgggtcttg
    14581 aaggtgccaa ttctgcagag cttgcaccag aaacaaaata ccctatcatt gatatcatgc
    14641 gtgatcagat tgatgttgag gatatggggg gaacccttcg tttgggactt tatccgtcta
    14701 aactaaaacg tggctctaag gcagcagctg cttatcacaa tcaagaagtg gtgcaacgcc
    14761 gtcaccgtca ccgctatgag tttaacaacg ctttccgtga gcagtttgag gcagcaggtt
    14821 ttgtcttctc aggagtttct ccagacaatc gtttggtaga aatcgtggaa attccagaaa
    14881 ataaattctt tgtggcttgt cagtatcacc ctgaactgtc aagccgtcca aatcgcccag
    14941 aagagctcta cactgccttt gtcactgcag cagttgagaa cagcaattag caaaatcaga
    15001 acctttgaga agaatctcag aggttttttg catacgatgg atattgcagt atatctgagg
    15061 taggagtcct ttgtatgtgc ctgctaccct tgaaatcaat agcgactccc tcttgccctg
    15121 tgctagtgaa tggatttatc agtatattga aatgaaataa aatctgaaca aattaattcg
    15181 gaaagccaaa tcaatttcta gcaaagtttt aggaactgga ttgtatagtg aattgaaata
    15241 agatatgaac atctctatca gaaaagtcaa attaatttat agaaatattt tagcagtcaa
    15301 ggtgtactgt tatagattca atacattata cttttttaat ttaatccact atagtaaaat
    15361 gaaataataa caggataaat cgatcaggac agtcaaatcg atttctaaca atgttttaga
    15421 aatagaggtg tactattcta gtttcaatat actatatatc tgcagaatac tctttcagtt
    15481 tcattcagcc tagtcttgct taggaaaaag taaaaagcca tccaattatg atggatagct
    15541 tcttggttct tagatttgtt ctgcaaaaac cttttcagca aggttcatag cgtggtctga
    15601 tacacgagtg tagtgtgaga tgatgtcgat aaagttgacc ccagcttgtg ttgaacattc
    15661 gcctttgttg aggcgtttga tgtgggtttt tctgagaaca cgttctatct tattgattgc
    15721 ttcatgacgt tctacaagac tgcgtgcttt ttcaatatca ttgttttcca cactatccag
    15781 agcatctttg ataaagtcac tagtttgacg gtaaacttcc tctaattctt tcaaggcggc
    15841 atcagaaaat tcaacatttt tccgttgaag atagtcagtc agattgagta gagcctccgt
    15901 gtggtctcca atccgttcca aatcacggga ggaatcaagg atattggtaa gcacttcact
    15961 ttctttttgg ctgagagctt cgcttgaaag ggcaatgaga taacgtgtta attgctcatc
    16021 gatggtgtta attgcttctt cggttttatg ccctttttca gcaacttttt catccaagtc
    16081 aatgatgtac ttataggaaa ggtcaaaggc tttagcagcg tagtttccta agtgcaagag
    16141 ctctttttta gcatttccta gagcgataga tggggcttgt ttgatgaaat gttcatcaag
    16201 atataagggt tcgtatttga caacctcgtc ctctccagga ataatcttgg ttacaaagta
    16261 agccagagct ccgataaatg gaaattggac aatggtgttg gtaatattaa aggttccgtg
    16321 agcaaaggcg atggtcattt ccggtgctag atttagcgta gcttcaaacc aatgaatcag
    16381 gacagtaaaa ggaactagaa aaataacgca gacgactgtt ccgataacgt tgaaggcaac
    16441 atgagctcct gctacccgtt tagctgcaat attagcccct aaagaggcaa tgatggctgt
    16501 aatggttgtc ccgatattgt caccaaatag aactggcaaa gctccctgta ggtcaattag
    16561 attgccggcg tagaggtttt gtaaaatccc aatggtagcc gaagaagctt gaattagcaa
    16621 ggtcaagccg gtaccgacaa agacacccaa aacaggattc ttacttagct caatcatata
    16681 gtccttaaag acctgtaaat ccttgagtgg agccattgcg ccgctcatga gattgagggc
    16741 aaaaaagata ccaccgacac caaagaggat gcgtccgata ttattgactg tccgattttt
    16801 cgtaaaaaag agacagacgg caccgataaa gagcataggt agggcatagt tacctaattt
    16861 aaaaccgatg agaaaggatg tgacagttgt cccaatatta gcacccatga caatcccgat
    16921 agcctgacgt aaggttaaga gaccggcact gaccaggccg actgtaataa ctgttacacc
    16981 agaactagac tgaattagag cagtcatccc aataccaacc agaactccaa agaaaggatt
    17041 actagtatat ttgtcaatgt aaaaacgaag gcgatctcca gcagcttgtt gcaaaccgtc
    17101 tcccatggtc ttgatgctat ataagaatag ccccagacca cctaaaaagt gaaataaaat
    17161 ttcctgccaa ttaatggaca tttctttttc ctccgaaaaa taatcgcgga atatctccta
    17221 ttctatttta aaggataaaa gtaaatctaa caagtgttaa tgtcaagttt gtaaaagaaa
    17281 attataattg ataagtgtat tatttttaag agtttttaac cttattttag atttgtgcaa
    17341 acagttattg ttttttgtct aaaaatgttt taggataaaa atgtgattat catggttcta
    17401 gagtttgata aaaaactaca gaatataaag cgctttcttt aaaacaacaa aatcaatctt
    17461 ttaggaggag aaaatgaaga atccattttt tgaaagacgt tgtcgttaca gtattcgtaa
    17521 gttatcagta ggagcctgct cgctgatgat tggtgctgtt ttatttgctg gtccagcctt
    17581 ggctgaagaa actgcagttc ctgaaaatag cggagctaat acagagcttg tttcaggaga
    17641 gagtgagcat tcgaccaatg aagctgataa gcagaatgaa ggggaacatg ctagagaaaa
    17701 caagctagaa aaggcagaag cagctagcgc agctaaacca gaggaaaaag caggtgaggt
    17761 ggttgcagaa acaccatctg cagaagcaaa acctaagtct gacaaggaaa cagaagcaaa
    17821 gcccgaagca actaaccaag gggatgagtc taagccagca gcagaagcta ataagactga
    17881 aaaagaagtc cagccagatg tccctaaaaa tacagaaaaa acattaaaac caaaggaaat
    17941 caaatttaat tcttgggaag aattgttaaa atgggaacca ggtgctcgtg aagatgatgc
    18001 tattaaccgc ggatctgttg tcctcgcttc acgtcggaca ggtcatttag tcaatgaaaa
    18061 agctagcaag gaagcaaaag ttcaagcctt atcaaacacc aattctaaag caaaagacca
    18121 tgcttctgtt ggtggagaag agttcaaggc ctatgctttt gactattggc aatatctaga
    18181 ttcaatggtc ttctgggaag gtctcgtacc aactcctgac gttattgatg caggtcaccg
    18241 taacggggtt cctgtatacg gtacactctt cttcaactgg tctaatagta ttgcagatca
    18301 agaaagattt gctgaagctt tgaagcaaga cgcagatggt agcttcccaa ttgcccgtaa
    18361 attggtagac atggccaagt attatggcta tgatggctat ttcatcaacc aagaaacaac
    18421 tggagatttg gttaaacctc ttggagaaaa gatgcgccag tttatgctct atagcaagga
    18481 atatgctgct aaggtaaacc atccaatcaa gtattcttgg tacgatgcca tgacctataa
    18541 ctatggacgt taccatcaag atggtttggg agaatacaac taccaattca tgcaaccaga
    18601 aggagataag gttccggcag ataacttctt tgctaacttt aactgggata aggctaaaaa
    18661 tgattacact attgcaactg ccaactggat tggtcgtaat ccttatgatg tatttgcagg
    18721 tttggaattg caacagggtg gttcctacaa gacaaaggtt aagtggaatg acattttaga
    18781 cgaaaatggg aaattgcgcc tttctcttgg tttatttgcc ccagatacca ttacaagttt
    18841 aggaaaaact ggtgaagatt atcataaaaa tgaagatatc ttctttacag gttatcaagg
    18901 agaccctact ggccaaaaac caggtgacaa agattggtat ggtattgcta acctagttgc
    18961 ggaccgtacg tcagcggtag gtaatacttt tactacttct tttaatacag gtcatggtaa
    19021 aaaatggttc gtagatggta aggtttctaa ggattctgag tggaattatc gttcagtatc
    19081 aggtgttctt ccaacatggc gctggtggca gacttcaaca ggggaaaaac ttcgtgcaga
    19141 atatgatttt acagatgcct ataatggcgg aaattccctt aaattctctg gtgatgtagc
    19201 cggtaagaca gatcaggatg tgagacttta ttctactaag ttagaagtaa ctgagaagac
    19261 caaacttcgt gttgcccaca agggaggaaa aggttctaaa gtttatatgg cattctctac
    19321 aactccagac tacaaattcg atgatgcaga tgcatggaaa gagctaaccc tttctgacaa
    19381 ctggacaaat gaagaatttg atctcagctc actagcaggt aaaaccatct atgcagtcaa
    19441 actatttttc gagcatgaag gtgctgtaaa agattatcag ttcaacctcg gacaattaac
    19501 tatctcggac aatcaccaag agccacaatc gccgacaagc ttttctgtag tgaaacaatc
    19561 tcttaaaaat gcccaagaag cggaagcagt tgtgcaattt aaaggcaaca aggatgcaga
    19621 tttctatgaa gtttatgaaa aagatggaga cagctggaaa ttactaactg gctcatcttc
    19681 tacaactatt tatctaccaa aagttagccg ctcagcaagt gctcagggta caactcaaga
    19741 actgaaggtt gtagcagtcg gtaaaaatgg agttcgttca gaagctgcaa ccacaacctt
    19801 tgagtggggg atgactgtcc aagacacaag tcttccaaga cctctagcag aaaacatcgt
    19861 tccaggggct actgttatcg gaagtacctt cccagataca gaaggtggag aaggtattga
    19921 aggtatgttg aacggtacca ttactagctt gtcagataaa tggtcttcag ctcagttgag
    19981 tggtagtgtg gatattcgtt tgaccaagcc acgtaccgtt gttagatggg tcatggatca
    20041 tgcaggggct ggtggtgagt ctgttaacga tggcttgatg aacaccaaag actttgacct
    20101 ttattataaa gatgcagatg gtgagtggaa gctagctaag gaagtccgtg gcaacaaagc
    20161 acacgtgaca gatatcactc ttgataaacc aatcactgct caagactggc gcttgaatgt
    20221 tgtcacttct gacaatggaa ctccatggaa ggctattcgt atctataact ggaaaatgta
    20281 tgaaaagctt gatactgaga gtgtcaatat tccgatggcc aaggctgcag cccgttctct
    20341 aggcaataac aaggtacaag ttggctttgc agatgtaccg gctggagcaa ctattaccgt
    20401 ttatgataat ccaaattctc aaactccgct tgcaaccttg aagagcgaag ttggaggaga
    20461 cctagcaagt gcaccattgg atttgacaaa tcaatctggt cttctttatt atcgtaccca
    20521 gttgccaggc aaggaaatta gtaatgtcct agcagtttcc gttccaaaag atgacagaag
    20581 aatcaagtca gtcagcctag aaacaggacc taagaaaaca agctacgccg aaggggagga
    20641 tttggacctt agaggtggtg ttcttcgagt tcagtatgaa ggaggaactg aggacgaact
    20701 cattcgccta actcacgcag gtgtatcagt atcaggtttt gatacgcatc ataagggaga
    20761 acagaatctt actctccaat atttgggaca accagtaaat gctaatttgt cagtgactgt
    20821 cactggccaa gacgaagcaa gtccgaaaac tattttggga attgaagtaa gtcagaaacc
    20881 gaaaaaaaat tacctagttg gtgatagctt agacttgtct gaaggacgct ttgcagtggc
    20941 ttatagcaat gacaccatgg aagaacattc ctttactgat gagggagttg aaatttctgg
    21001 ttacgatgct caaaagattg gtcgtcaaac cttgacgctt cgttaccaag gtcatgaagt
    21061 caactttgat gttttggtat ctccaaaagc agcattgaac gatgagtacc tcaaacaaaa
    21121 attagcagaa gttgaagctg ctaagaacaa ggtggtctat aactttgctt caccagaagt
    21181 aaaagaagct ttcttgaaag caattgaagc ggccgaacaa gtgttgaaag accatgaaat
    21241 tagcacccaa gatcaagtca atgaccgact taataaattg acagaagctc ataaagctct
    21301 gaatggtcaa gagaaattta aggaagaaaa gacagagctt gatcgtttaa caggtgaggt
    21361 tcaagaactc ttggatgcca aaccaaacca tccttcaggt tctgccctag ctccgcttct
    21421 tgagaaaaac aaggtcttgg ttgaaaaagt agatttgagt ccagaagagc ttgcaacagc
    21481 gaaacagagt ctaaaagatc tggttgcttt attgaaagaa gacaagccag cagtcttttc
    21541 tgatagtaaa acaggtgttg aagtacactt ctcaaataaa gagaagactg tcatcaaggg
    21601 tttgaaagta gagcgtgttc aagcaagtgc tgaagagaag aaatactttg ctggagaaga
    21661 tgctcatgtc tttgaaatag aaggtttgga tgaaaaaggt caagatgttg atctctctta
    21721 tgcttctatt gtgaaaatcc caattgaaaa agataagaaa gttaagaaag tatttttctt
    21781 acctgaaggc aaagaggcag tagaattggc ttttgaacaa acggatagtc atgttatctt
    21841 tacagcacct cactttactc attatgcctt tgtttatgaa tctgctgaaa aaccacaacc
    21901 tgctaaacca gcaccacaaa acacagtcct tccaaaacct acttatcaac cggcttctga
    21961 tcaacaaaag gctcctaaat tggaagttca agaggaaaag gttgcctttc atcgtcaaga
    22021 gcatgaaaat gctgagatgc tagttgggga acaacgagtc atcatacagg gacgagatgg
    22081 actgttaaga catgtctttg aagttgatga aaacggtcag cgtcgtcttc gttcaacaga
    22141 agtcatccaa gaagcgattc cagaaattgt tgaaattgga acaaaagtaa aaacagtacc
    22201 agcagtagta gctacacagg aaaaaccagc tcaaaataca gcagttaaat cagaagaagc
    22261 aagcaaacaa ttgccaaata caggaacagc tgatgctaat gaagccctaa tagcaggctt
    22321 agccagcctt ggtcttgcta gtttagcctt gaccttgaga cggaaaagag aagataaaga
    22381 ttaaatatcg aaaaatcttg tgaaatcttt ccttatattt ctaaagtgtg atataatagt
    22441 ttcgaataaa ataaataaag gagtttttgt aacatggcaa aacttactgt taaagacgtt
    22501 gacttgaaag gtaaaaaagt cctcgttcgt gttgacttca acgtaccatt gaaagatggc
    22561 gtaatcacta acgataaccg tatcacagca gctcttccaa ctattaagta catcatcgaa
    22621 caaggtggac gtgcaattct tttctctcac cttggacgtg tgaaagaaga agctgataaa
    22681 gctggtaaat cacttgctcc tgtagcagct gacttggcag caaaacttgg tcaagatgtt
    22741 gttttcccag gtgtcactcg tggtgctgaa ttggaagcgg caatcaacgc tcttgaagat
    22801 ggacaagttc tcttggttga aaacactcgt tacgaagatg ttgacggcaa gaaagaatct
    22861 aaaaacgatc ctgaacttgg taaatactgg gcatcacttg gagatggtat cttcgtaaac
    22921 gatgcattcg gtacagctca ccgtgcacac gcatctaacg ttggtatctc agcaaacgtt
    22981 gaaaaagcag ttgctggttt ccttcttgaa aacgaaattg cctacatcca agaagcagtt
    23041 gaaactccag aacgtccatt cgtggctatc cttggtggtt caaaagtttc agacaagatc
    23101 ggtgttatcg aaaacttgct tgaaaaagct gataaagtcc ttatcggtgg tgggatgact
    23161 tacacattct acaaagcaca aggtatcgaa atcggtaact cacttgtaga agaagacaaa
    23221 ttggatgttg cgaaagctct tcttgaaaaa gcaaatggta aattgatctt gccagttgac
    23281 tcaaaagaag ctaacgcatt tgctggttac actgaagtgc gtgacactga aggtgaagca
    23341 gtttctgaag gcttccttgg tcttgacatc ggtccaaaat ctatcgccaa atttgacgaa
    23401 gctttgactg gtgccaaaac agttgtatgg aacggaccta tgggtgtatt tgaaaaccca
    23461 gatttccaag ctggtacaat cggtgtgatg gacgctatcg tgaaacaacc aggagttaaa
    23521 tcaatcatcg gtggtggtga ctcagctgcc gcagcgatta accttggccg tgcagacaag
    23581 ttctcatgga ttagtacggg tggtggagca tcaatggaac ttcttgaagg taaggttctt
    23641 ccaggacttg cagccttgac agaaaaataa gattttataa acaaatcaaa gaagagaggg
    23701 atgaaagttc ctcttttctt ttgcttaaaa taaaaacgct tcctctcaac tattactcat
    23761 aaaaatcacc gatttatgat aaaatggaaa tagaaagttg agattatgag ttattttaaa
    23821 aaatataaat tcgataaatc ccagttcaaa cttggtatgc gaacctttaa aacaggtatt
    23881 gctgtttttc tagttctctt gatttttggc ttttttggct ggaaaggtct tcaaattggt
    23941 gctttgacag ccgtttttag cctgagggag agttttgatg agagtgttca ttttgggact
    24001 tcgcgtattc taggaaatag tatcggtgga ctctatgcct tggtcttctt cttattaaat
    24061 acctttttcc acgaagcctt ttgggtgacc ttggtagttg ttccaatctg caccatgtta
    24121 accattatga caaatgtagc catgaataac aaagcagggg ttattggtgg tgtagcagct
    24181 atgttaatca ttaccctatc aattccaagt ggtgagacaa tttcgtacgt gtttgtgcgt
    24241 gtatcagaaa cgtttatggg agtttttgtt gcaattatcg taaattacga tattgatcgt
    24301 attcgtctct ttttagagaa aaaagaaaaa taatgttaca ttttataaca ttatcaattg
    24361 acgtttgtct ttttttagac tataatagac agaaagaagg aaattgtaaa tgaaggaaaa
    24421 agaatttcgc cgaaatatgg ctgtttttcc tatcggcagt gttatgaagt tgaccgatct
    24481 atcggcgcgt cagattcgtt attatgaaga tcaagagttg atcaagcccg atcgaaacga
    24541 aggaaatcgt cgcatgtatt ccttgaatga catggatcgt ctgcttgaaa tcaaagatta
    24601 tatctctgaa ggttataata tcgctgccat taagaaaaaa tatgctgaac gtgaagcgaa
    24661 atccaagaaa gcggttagtc agactgaagt acgtcgtgca cttcacaatg aactcctcca
    24721 acaggggcgt tttgcttcag tacagtcacc ttttggtcgc ggttaggcaa ccgcaagtag
    24781 tcatagatta aggagaaaac ttatgccaat cacagctgca gatattcgtc gtgaagtcaa
    24841 ggaaaaaaat gttaccttta ttcgtcttat gttctcagat attttgggaa ccatgaaaaa
    24901 cgtcgaaatt cctgctacag atgaacagtt agataaggtc ttgtcgaaca aggttatgtt
    24961 tgatggatct tctattgaag gttttgtacg tatcaatgag tcggatatgt acttgtatcc
    25021 ggacttggat acatggacag tcttcccttg gggagatgaa aatggaagtg ttgcaggtct
    25081 gatctgtgat gtctatacaa cagaaggtga accatttgcg ggtgaccctc gtggtaattt
    25141 gaaacgagct cttcgtcaca tggaagaagt tggattcaaa tccttcaacc ttggtccaga
    25201 gccagaattc ttcctattta agttggatga aaatggggac ccaacacttg aagtgaatga
    25261 caagggtggc tactttgatt tggcacctac tgaccttgcg gacaacacac gtcgtgagat
    25321 tgtgaatgtc ttgaccaaaa tgggatttga agtagaagcg agtcaccacg aggttgcggt
    25381 tggacagcat gagattgact ttaagtacga tgaagttctc cgtgcttgtg ataagattca
    25441 aatctttaag cttgttgtta aaaccattgc tcgcaaacac ggactttacg caacatttat
    25501 ggcgaagcca aaatttggta ttgctggatc aggtatgcac tgtaatatgt ccttgtttga
    25561 tgcagaagga aataacgcct tctttgatcc aaatgatcca aaaggaatgc agttgtcaga
    25621 aacagcttac catttcctag gcggtttgat caagcatgct tacaactata ctgccatcat
    25681 gaacccaaca gttaactcat acaaacgttt ggttccaggt tatgaagcgc ctgtttacat
    25741 tgcttgggct ggtcgtaacc gttcgccact tgtgcgcgta cctgcttcac gtggtatggg
    25801 aactcgtctt gagttgcgtt cagtggatcc aatggcgaac ccttacgttg ctatggctgt
    25861 tcttttggaa gttggtttgt atggtattga aaataaaatc gaagcaccag ctcctatcga
    25921 agaaaatatc tacatcatga cagcagaaga gcgcaaggaa gctggtatta cagaccttcc
    25981 atcaactctt cacaacgctt tgaaagcttt gacagaagat gaagtggtta aagctgctct
    26041 cggagatcac atctatacta gcttccttga agccaaacga atcgaatggg caagttatgc
    26101 aaccttcgtt tcacaatggg aaattgataa ttatttagac ctttactaat actaatatag
    26161 aagaaagatt gcctgtgggt aaacagtcac acagcagtct aagccatcaa atacctataa
    26221 cccttaaaat agcgatttcg gtcgctattt atttttcgaa aaatcctctt gactcttttg
    26281 cgaattagat ttatcatcag acgtgtagaa gaaaatcaag gagtcactat ggaaaaattc
    26341 aatttcaaaa acaatatcgg gcaagaaaac aagttacttc aaatcgaaat ctacaagttt
    26401 actaactttt gcaagcttca aaattacaca tctgtaaata ttttttcgaa agatatattt
    26461 gaagccatag taaattgaga ctagaatagt acactacatc tgctaaaaat ttctagaaat
    26521 taatttaact ttcctaatcg atttgtttac atcttatttc aatctactat attatctcaa
    26581 taaatgatga tggtgactgt tttgtttgtg cttgtcgatt tttgaatcct gatcgagcgg
    26641 aagaatattt ctgccgaggt tgtcaagggg aaaatcctga agatattgaa ttttatgatg
    26701 aacaattaca ggcagaaaag gtggaggctt tgaatattcg attggaagta aaaaattgaa
    26761 ttatctacaa tctcatcaac aataaaccta tttcaacccc attatctata ggcgtatttt
    26821 tacgcctttt tatatacttg aagcacataa gaaacattat gggaagtttg ctgatggaag
    26881 cactcaagaa attgatgttc cttatgatat tcctgatact tgggagtggg tgaggttttc
    26941 tacattggtt gaaattgtca gaggtggctc tccacgacca atcaaagatt atcttacttc
    27001 tgaagtagat ggaataaatt ggataaaaat aggtgatact gaaaagggtg aaaagtatat
    27061 aaataatgtt aaagaaaaaa tcaaaaaatc agggcttaac aaaactagat ttgtaaaaaa
    27121 aggtacattt ttgttaacta attctatgag ttttggtaga ccttatattt tgaatgttga
    27181 tggtgcaata cacgatggat ggttggctat ttcgaactat gaaaactcat taaataaaga
    27241 ttacctattc tatattcttt catcaaatgt agtttattct caatttctat ctctaattag
    27301 tggagctgtt gtgaaaaact tgaatagtga taaagttgct tctattctta tccctctccc
    27361 cccactatcc gaacaacaac gcataataga agcaatcgaa tcagctttag aaaaagtaga
    27421 tgaatatgct gaaagttata atagactaga acagctagat aaagaatttc cagataaact
    27481 aaaaaaatct attcttcaat atgctatgca aggaaaatta gttgaacaag acccaaatga
    27541 tgaatcagtc gaagttttac ttgaaaaaat acgagcagaa aaacaaaaac tctttgaaga
    27601 aggcaagatt aaaaagaaag atttggacat ttctattgtt tcccaaggag atgataactc
    27661 ttattatgag gaagtacctt gtgaaatacc agaaagttgg gaatgggtga ggttaaacga
    27721 tataacctcc tacattcaaa gagggaagtc tccaaaatat tcaaatattc ctatataccc
    27781 tgtgattgct cagaaatgta atcaatggtc tggcttttct attgacttag caagatttat
    27841 tgatcccgaa acagttcata gttaccagaa agaaagatta ctaagagatg gtgatttaat
    27901 gtggaattca acaggtctag gtacgttagg acgattagct atttaccatg aaaataaaaa
    27961 tccttatggc tgggctgttg cagatagcca tgttacagtt ataagagtct tatcgggagt
    28021 tattaattgc catttcatct ataattttct ttcctctcct attgttcagt cagttattga
    28081 agaaaaagcg tcgggtagta caaaacaaaa agaattatta acgaaaacta taaaagaata
    28141 tttaatcccc ctcccacccc ttcctgaaca atccagaatc gttgataaaa tcgaacaatt
    28201 tttcgcccat attgacgcac taatttagta taatagtttt acgtgcggtg gaatttctat
    28261 caaacataag ataggagttt tacgttgaaa gattttgaat tacatttgaa gaaagctggt
    28321 ttagctgaaa atactgtgcg ttcgtacctt tatggtgttc gatttttctt ggagaattac
    28381 gaactcaaaa tggaagattt atttgaatac aaaggatatt tattagacaa ttttaaacct
    28441 aaaactgtca atttgcgact acaaggagta aataaatatc tggcttttat tggtcatgat
    28501 gatttgaaat taaaatttgt caaagtacag caaaaaccat ttttagaaga tgtcattagc
    28561 catgctgatt acctttttct taaacgtagt ttgaaaaaag atggtatttt aaaatggcat
    28621 tttgtcgttt ggtttttagg tgcaactggg gctcgtgtaa gtgaacttat taaactaaaa
    28681 gtagagcatg tagaaatcgg ttattttgac atttactcaa aaggtggtaa aattcgtaga
    28741 ctgtacattc ctaaaaaatt aagaaatagt tgtcttagtt ggttagaatc agaaaatcgc
    28801 cgaagtggtt atttattttt gaataaattt aacgaaccaa ttacagcaag aggagttgct
    28861 caacagttaa aaaattatgc tgataaatac aaaatgaatc ctaaagtaat ttaccctcat
    28921 tcttttaggc atttatttgc taagaatttt ttagcgaagt ataatgatat tgccttgctt
    28981 gcagatttga tgggacacga aagtatagaa actactcgaa tttatctaag gaa
//
LOCUS       DAYYQN010000024        28630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYYQN010000024 DAYYQN010000000
VERSION     DAYYQN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.024"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            333..1496
                     /locus_tag="ACHBRQ_000975"
     CDS             333..1496
                     /locus_tag="ACHBRQ_000975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9592667.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHCFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1493..2170
                     /gene="aroD"
                     /locus_tag="ACHBRQ_000976"
     CDS             1493..2170
                     /gene="aroD"
                     /locus_tag="ACHBRQ_000976"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9592668.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2160..3014
                     /locus_tag="ACHBRQ_000977"
     CDS             2160..3014
                     /locus_tag="ACHBRQ_000977"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9592669.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3033..4100
                     /gene="aroB"
                     /locus_tag="ACHBRQ_000978"
     CDS             3033..4100
                     /gene="aroB"
                     /locus_tag="ACHBRQ_000978"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9592670.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4110..5276
                     /gene="aroC"
                     /locus_tag="ACHBRQ_000979"
     CDS             4110..5276
                     /gene="aroC"
                     /locus_tag="ACHBRQ_000979"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9592671.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5286..6389
                     /locus_tag="ACHBRQ_000980"
     CDS             5286..6389
                     /locus_tag="ACHBRQ_000980"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9592672.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEYLITENTDYTVVIK"
     gene            6400..6738
                     /locus_tag="ACHBRQ_000981"
     CDS             6400..6738
                     /locus_tag="ACHBRQ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HFM9592673.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6830..8113
                     /gene="aroA"
                     /locus_tag="ACHBRQ_000982"
     CDS             6830..8113
                     /gene="aroA"
                     /locus_tag="ACHBRQ_000982"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9592674.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8106..8582
                     /locus_tag="ACHBRQ_000983"
     CDS             8106..8582
                     /locus_tag="ACHBRQ_000983"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9592675.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8579..9427
                     /gene="pheA"
                     /locus_tag="ACHBRQ_000984"
     CDS             8579..9427
                     /gene="pheA"
                     /locus_tag="ACHBRQ_000984"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9592676.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9424..10698
                     /gene="psr"
                     /locus_tag="ACHBRQ_000985"
     CDS             9424..10698
                     /gene="psr"
                     /locus_tag="ACHBRQ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HFM9592677.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10977..11822
                     /locus_tag="ACHBRQ_000986"
     CDS             10977..11822
                     /locus_tag="ACHBRQ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HFM9592678.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11812..12915
                     /locus_tag="ACHBRQ_000987"
     CDS             11812..12915
                     /locus_tag="ACHBRQ_000987"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9592679.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12917..13903
                     /locus_tag="ACHBRQ_000988"
     CDS             12917..13903
                     /locus_tag="ACHBRQ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9592680.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13919..15352
                     /locus_tag="ACHBRQ_000989"
     CDS             13919..15352
                     /locus_tag="ACHBRQ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HFM9592681.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15381..16415
                     /locus_tag="ACHBRQ_000990"
     CDS             15381..16415
                     /locus_tag="ACHBRQ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9592682.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16615..17352
                     /gene="mecA"
                     /locus_tag="ACHBRQ_000991"
     CDS             16615..17352
                     /gene="mecA"
                     /locus_tag="ACHBRQ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9592683.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17503..18789
                     /locus_tag="ACHBRQ_000992"
     CDS             17503..18789
                     /locus_tag="ACHBRQ_000992"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFM9592684.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18791..19660
                     /gene="thrB"
                     /locus_tag="ACHBRQ_000993"
     CDS             18791..19660
                     /gene="thrB"
                     /locus_tag="ACHBRQ_000993"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFM9592685.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19736..19888
                     /locus_tag="ACHBRQ_000994"
     CDS             19736..19888
                     /locus_tag="ACHBRQ_000994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592686.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEVSSRHCFEVADRTDDVS
                     NIYTVR"
     gene            19955..20893
                     /gene="msrB"
                     /locus_tag="ACHBRQ_000995"
     CDS             19955..20893
                     /gene="msrB"
                     /locus_tag="ACHBRQ_000995"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9592687.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20951..22675
                     /locus_tag="ACHBRQ_000996"
     CDS             20951..22675
                     /locus_tag="ACHBRQ_000996"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592688.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VLVAILTIVIVGLSRLVNPFYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22677..24428
                     /locus_tag="ACHBRQ_000997"
     CDS             22677..24428
                     /locus_tag="ACHBRQ_000997"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592689.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
     gene            24820..26079
                     /locus_tag="ACHBRQ_000998"
     CDS             24820..26079
                     /locus_tag="ACHBRQ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HFM9592690.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHIHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26155..26290
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26329..26829
                     /gene="rplJ"
                     /locus_tag="ACHBRQ_000999"
     CDS             26329..26829
                     /gene="rplJ"
                     /locus_tag="ACHBRQ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9592691.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26905..27273
                     /gene="rplL"
                     /locus_tag="ACHBRQ_001000"
     CDS             26905..27273
                     /gene="rplL"
                     /locus_tag="ACHBRQ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9592692.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27460..27630
                     /locus_tag="ACHBRQ_001001"
     CDS             27460..27630
                     /locus_tag="ACHBRQ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592693.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27630..27878
                     /locus_tag="ACHBRQ_001002"
                     /pseudo
     CDS             27630..27878
                     /locus_tag="ACHBRQ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27892..28232
                     /locus_tag="ACHBRQ_001003"
                     /pseudo
     CDS             27892..28232
                     /locus_tag="ACHBRQ_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28225..28605
                     /locus_tag="ACHBRQ_001004"
     CDS             28225..28605
                     /locus_tag="ACHBRQ_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9592694.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
BASE COUNT         8501 a         5298 c         6437 g         8394 t
ORIGIN      
        1 aaattttcca gatacctatt gaagcagatt tatttcttca tctttcttct actacctttg
       61 tcactaaatt tgacagacac atattgctga ttcaacctct ctacctataa tctcacagtt
      121 aacctgaggc tagtttcata gtttgctatt tgattttcgt tgataaaaag aaatgctcct
      181 ccaagacgga agagcatttt ttattgtgat tcttacacat ttacttccta gttttgcagg
      241 aagtctagta taccattttt taaaagaaga gtaaaaaagc aagggtagtt tcttttttga
      301 gaattgtcta aaagtttgat ataatggtag ttatgaatag aataagagtc agcaaaaggg
      361 ttgaaaagaa gcttgctaag gggctagttt tactagaagc cagtgatctt gagaatgtca
      421 atcttaagga tcaggaagta gaggtgcagg gtcaggaagg aaactttctt ggaactgcct
      481 acctttctca gcaaaacaag ggcttgggct ggtttgtcag caaagacaag gtggccttca
      541 atcaagcttt ctttgaaacg ttgtttagaa aagccaaaga aaagagaaac gcctactatc
      601 aagatgattt gacaactgcc tttcgtctct ttaatcaaga gggagatggc tttgggggtc
      661 tgacagtgga cctttatggc gactacgccg tcttttcttg gtataactct tatgtttatc
      721 agattcgtca gactatatca gaagccttta gacaggtttt ccctgaggtt ttaggggctt
      781 atgagaaaat ccgctttaag ggtttggact atgaatctgc ccatgtttat ggtcaagaag
      841 cacctgactt tttcactgtt ttagaaaatg gtgtcctgta tcaagtcttt atgaatgatg
      901 gcttgatgac aggaattttc ctagaccagc atgaggttcg cggtagttta gttgacggct
      961 tggctatggg taaatccttg ctcaatatgt tttcctacac agcggctttt tcagtagctg
     1021 cggccatggg aggagctagc cagacaactt ctgttgatct agccaaacgt tcacgagaat
     1081 tgtctcaagc gcattttcag gcaaatgggc tcagcacaga cgagcattgt tttatagtca
     1141 tggatgtctt tgagtatttc aaatatgcca aacgtaaaaa cttgacctac gatgtgattg
     1201 tcctagatcc gcctagcttt gctcggaata aaaaacaaac tttctctgtg gccaaggatt
     1261 atcacaagtt gatttcccag agtcttgaga ttttaaatcc gggagggatt atcattgcca
     1321 gtaccaatgc tgccaatgtt tcccgtcaga aatttacaga acaaattgat aaaggctttg
     1381 caggaagaag ttaccagatt ttaaacaaat atggtcttcc agcagatttt gcctataata
     1441 aaaaagatga aagtagtaat tacctcaagg tgattagtat gaaggttagt aaatgaaatt
     1501 aatcgtttca gtaatgccaa gaagtttaga ggaggctcag gctctggatg ccacgaggta
     1561 cctggatgcc gacatcattg aatggcgtgc cgactatctg cctaaagaag cgattttgca
     1621 ggtagctcca gccatttttg aaaaattcgc aggccgtgag ttggttttca cgctacgaac
     1681 tcgctccgaa ggtggagaaa tcgacctttc tccagaagag tatatccatc taatcaagga
     1741 agttgcgcaa ctctatcaac cagactatat tgattttgag tactatagct acaaggatgt
     1801 ttttgaggaa atgctggact tcccaaatct tgttttgagt taccataatt tccaagaaac
     1861 acctgagaac atgatggaaa tcttgtcaga gttgacgatc ctaaatccaa aacttgttaa
     1921 ggttgcggtg atggctcaca cggagcagga tgtcttagac ttgatgaact atacacgagg
     1981 ctttaaaacc ctcaatcctg aacaggaata tgtgaccatt tctatgggta aggtgggcaa
     2041 ggtctctcgt atcactgcgg atgtgactgg ttcgagttgg tcttttgcca gtctggatga
     2101 ggttagtgcc cctgggcaga tttctctagc tagcatgaaa aaaatcaggg agattttgga
     2161 tgaagcttga tggctataca cgtttagctg cagttgttgc caatcctatt aagcattcta
     2221 tttctccctt catccacaat agagcctttg aggcgacagc taccaacggt gcttatgtgg
     2281 cttgggagat tgaagcgagt gacttggtag aaacagtggc caatattcgt cgctaccaga
     2341 tgtttggcat caatctgtcc atgccctata aggagcaggt gattccttat ttggatgagc
     2401 tgagcgatga agcgcgcttg attggtgcgg ttaatacggt tgtcaatgag aatggcaatt
     2461 taattggata taatacagat ggcaagggat tttttaagtg cttgccttct tttacaattt
     2521 caggtaaaaa gatgaccctg ctgggtgcag gtggtgcggc taaatcaatc ttggcacagg
     2581 ctattttgga tggcgtcagt cagatttcgg tctttgttcg ttccgtttct atggaaaaaa
     2641 caagacctta cctagacaag ttacaggagc agacaggctt taaagtggat ttgtgtgctt
     2701 tagaatatgt ttctgaactg caagcaagga ttgccgagtc ggatttgcta gttaatgcca
     2761 ccagtgtggg catggatggc caatcctctc ctgttcctga aaacatagtc ttaccagaaa
     2821 ctcttttagt tgcagatatc atataccaac cctttgaaac accgtttttg aaatgggcta
     2881 gaagtcaggg caatccagca gtcaatggtc tgggaatgtt gctctatcaa gctgcagaag
     2941 cttttcaact gtggacaggc aaggaaatgc cgacagaaga gatttggcag tctttaacag
     3001 aaaaatacca ataaagaaag ggagactctc ctatgaaaat cagaatcgat attcctcatc
     3061 atccttatga tattcagatt gaaaaaggtt gtatggccca ggctggtcag tggttgcgag
     3121 aactctggca accgcaaaag gtagtcattg tgacagataa ccatgtagcc tctctctatg
     3181 cagagaaggt caagctcagc ctagaagatg ctggttttca ggtagctgtt tttgatttct
     3241 tagaaggtga agaaagaaag aatttaacta ctgttcagaa agtctatgaa tttctagtca
     3301 agcaaggtct gactcgtagc gatggaatcg ttgctcttgg tggtggcgtt gttggggacc
     3361 tggctggctt tgtagcctct acctatatgc ggggtattca ctttgttcag attccgacta
     3421 gtttgacagc tcaggttgat tcttctatcg gtggaaagac aggcgttaac acaccatttg
     3481 ctaaaaatat ggtggggacc tttgcccaac cagatggggt tctgattgat ccacttgttc
     3541 ttgaaaccct cggaaaaaga gagttgattg aagggatggg tgaagttatc aagtatggct
     3601 tgattgagga tccagaactg tgggctctct tgacgggact gaatggttct gttgagagta
     3661 ttttggaaca tgcagagacc ttgattgaac attcttgtca ggtgaagcgc aagatggtgg
     3721 ttgaagatga gttggacaat ggtattcgtc tttacctcaa ttttggccac actattggcc
     3781 atgccatcga agcgactgcc ggttatggca aggtcatgca tggagaggct gttgccatgg
     3841 gaatggtaca gatttccaag gttgctgagg aaaaaggcct catgccagct ggcattaccc
     3901 aatctatcac agagatgtgt cagaaattcg gcttgcctgt tgactatgaa aattggaaag
     3961 ttgacaagct ttatcaggct cttactcatg acaagaaagc gcgtggtaac accttgaaat
     4021 tggtcttggt gccagagctt ggttcagcga ccattcaccc agtttctctg gaagagatga
     4081 aagactactt ggtaaaataa ggagagtgta tgagatattt aactgcagga gaatcacacg
     4141 gcccccgtct aacagctatt attgagggaa ttccagctgg acttccattg acagctgagg
     4201 atatcaatga ggatcttaga cgccgtcagg gtggctacgg tcgtggtggt cgtatgaaga
     4261 ttgagagtga ccaggttgtc tttacttcgg gcgttcgcca cgggaagacg acaggggcgc
     4321 ctattactat ggatgtcatc aataaggacc accagaaatg gctggacatc atgtctgcgg
     4381 aggacattga agaccgcctt aaaagcaagc ggaaaattac tcatcctcgc ccaggtcatg
     4441 ccgatttggt tggggggatt aagtaccgtt ttgatgattt gcgaaattct ttggagcgtt
     4501 catcagctcg tgaaaccacc atgcgggtgg cagttggtgc agtagccaaa cgtctcttgg
     4561 ctgagctgga tatggagatt gccaaccatg tcgtggtctt tggtggcaag gaaatcgatg
     4621 ttcctgaaaa tctgacagtc gctgaaatta agcaaagagc tgcccagtct gaagtttcta
     4681 ttgtcaacca agaacgagaa caggaaatca aggactatat tgaccaaatc aaacgtgatg
     4741 gtgataccat cggtggggtt gtggagacag tcgtcggagg cgttccagtt ggtcttggtt
     4801 cctatgtcca atgggataga aaattggatg caagattggc tcaagctgtt gtctctatca
     4861 atgcctttaa aggggtggaa tttggtcttg gctttgaggc tggttatcgt aaaggcagcc
     4921 aagttatgga tgaaattctc tggtctaaag aagacggtta tactcgccgt accaataatc
     4981 taggtggttt tgaaggtggt atgactaatg ggcaacccat cgttgttcgt ggggtcatga
     5041 aacccattcc tactctttat aaacctctta tgagtgtgga tatcgaaacc cacgaacctt
     5101 acaaggcaac cgtggagaga agtgatccga ctgctcttcc agctgcagga atggtcatgg
     5161 aagcagttgt agcaacggtt ctggcgcaag aaatcctcga aaaattctca tcagataatc
     5221 ttgaggaact aaaagaagcg gtagccaaac accgagacta tacaaagaac tattaaggag
     5281 ttcctatggc aaaaacaatc tatatcgcag gtcttgggtt gattggagcc tctatggcac
     5341 ttggtatcaa acgcgatcat ccagattatg aaattttagg ttataatcgt agtcaagctt
     5401 cgagagatat cgccttgaaa gaaggcatga ttgaccgtgc aacggatgat tttgctagtt
     5461 ttgctccttt ggcagatgtc attatcctca gcttgccaat caaacaaact attgctttca
     5521 ttaaggagtt ggccaatttg gatttgcgag aaggcgttat tatttcagat gctggttcga
     5581 ccaagtcaac cattgtggat gcggcggagc agtatttggc tggcaagtct gttcgctttg
     5641 tcggggccca tcccatggct ggtagtcaca agacaggggc tgcttcggca gatgtcaatc
     5701 tttttgaaaa tgcctattat atctttacac cttcaagcct gacaagtcag gacacgctta
     5761 aggaaatgaa ggatctgctt tcaggtcttc atgctcgttt tatcgagatt gatgccaagg
     5821 agcatgatcg tgtcacttct cagattagcc attttcctca tattttggct tctagtctca
     5881 tggagcagac tgcggtctat gctcaagagc atgagatggc aaggcgcttt gcggcaggtg
     5941 gttttcgaga tatgacccga attgcggaaa gcgagccagg aatgtggacc tccattctct
     6001 tgtccaatag cgagatcatt ctggatagaa ttgaggattt caaggaacgt ttggaagcga
     6061 ttggtcaggc cattagtaag ggagatgaag agcaaatttg gaactttttt aaccaagcgc
     6121 gtgaacaacg ccagaccatg gaaatccata agcgtggtgg tgtggatagc tcttacgacc
     6181 tctatgttga cgttcctgat gaagaagatg tcatcctgcg gattttggaa ctgctacgtg
     6241 gaacttcctt ggttaatatc cacatcaacg aggaaaatcg tgaggatatt cacggaatcc
     6301 tacaaatttc atttaaaaat gctcaagact tggaaagagc tgagtatctc ataacagaaa
     6361 ataccgacta tacagtcgtc atcaaataag gagaagaaca tgtcaaatat ttacgatagt
     6421 gcaaacgaac tcagtcgcgg tctacgcgga ttaccagaat acaaggctgt taaagcagct
     6481 aaagatgcga ttgcagcaga tgctgaggca agcaaaatct ttacagatta tcttgccttt
     6541 caagaggaaa ttcaaaaact agcacagaca ggtcaaatgc cagatgcgtc cttccaagct
     6601 aagatggaag gctttggtaa acagattcaa ggaaatagcc tcttgtcaga attctttacc
     6661 aagcaacaac aattggccat ttacctttct gacattgaaa aaattgtttt cgaaccagtt
     6721 tcagaattgc tgaaataaga aaagaactat cctaaagtca ggaattttta aggaatttct
     6781 ggctttttat gataaaatag gtaaaagtat tgcaagtatg aggtccagta tgaaactaaa
     6841 aacaaacatt cgccatttac atggtagtat ccgcgtccca ggtgacaagt ctatcagcca
     6901 ccgttccatt atctttggaa gtttggctga gggtgagacc aaggtttatg atattctgcg
     6961 aggtgaagac gttctttcga ccatgcaggt ttttcgtgac cttggtgttg aaattgagga
     7021 taaagatggg gttattacca ttcaaggtgt aggcatggct ggcttaaaag cgccgcaaaa
     7081 tgcccttaat atgggaaatt ctggcacctc gattcgcctg atttcaggtg tccttgctgg
     7141 tgcagatttc gaagtagaga tgtttggaga tgatagtctt tccaaacgtc ctatggaccg
     7201 tgtgaccctt ccactgaaaa aaatgggcgt cagcatttca gggcaaactg aacgagactt
     7261 gcctcccctt cgcttaaaag ggacgaaaaa cctaagacct attcattatg agttgccaat
     7321 tgcctctgcc caagtcaagt cagccttgat gtttgcagcc ttacaagcta agggggagtc
     7381 agttattatc gaaaaagaat acacccgtaa tcatactgaa gatatgttgc aacaatttgg
     7441 tggtcattta agtgtggatg gtaagaaaat cacagtccaa gggccacaaa aattgacagg
     7501 acagaaggtg gtcgtaccag gagatatttc cagtgcagcc ttttggttag tcgcaggttt
     7561 gattgctcca aattctcgtc tagtgctgca gaatgtgggg atcaacgaaa ctcgcaccgg
     7621 tattattgat gtcattcgtg ccatgggtgg aaaattggaa ataactgaaa tcgatccagt
     7681 cgctaaatct gcaaccttga ttgttgagtc ttctgacttg aaaggaacag agatttgtgg
     7741 cgctttgatt ccacgtttga ttgatgaatt gcctattatt gccctacttg cgacccaagc
     7801 ccaaggtgta acagttatta aagatgctga ggagctcaag gtcaaggaaa cagaccgtat
     7861 tcaggttgtg gcagacgcct taaatagtat gggagcagat attactccta cggcagatgg
     7921 gatgattatc aaagggaaat cagctcttca cggtgctaga gtcaatacgt ttggtgacca
     7981 ccgtatcggc atgatgacag ctatcgcagc cctcttggtt gcagatggag aggtggagct
     8041 tgaccgtgca gaagccatca ataccagcta tcctagtttc tttgatgatt tggagagctt
     8101 gattcatggc taaggtgtta ttagggttta tgggggctgg aaaatcgact attgcaagag
     8161 gcttggaccc taattacctt gatatggatg ctctgattga gaagcgccta ggtatgtcca
     8221 ttgcgaattt tttcgctgaa aagggagaag cagcctttcg tcaggtagaa tcagaagtcc
     8281 tagctgattt actacaaaca gaccaagtcg tgtcaactgg aggaggagtg gttatttctc
     8341 agagaaatcg tgacttactc aagactaata cagataacat ctacctgaaa gcagattttg
     8401 aaaccctcta ccaacgtatc gcagctgata aggacaatca gcgaccgctt tttctaaata
     8461 atagcaagga agaactagta gctatttttc aagaaagaca ggcttggtat gaggaagtgg
     8521 ctagtcgggt tttggatgtg accaagctaa gcccagagga aattatagag gaactaagat
     8581 gaaaattgct tatctaggtc ccaagggatc attttcacac cacgttgtgc agatagcttt
     8641 tcctcatgag gaattgcagg cttttgctaa cattacagat gtcatcaagg cttatgagca
     8701 aggattagtg gactattctg tggtgccagt tgaaaattct attgagggta gtgttcatga
     8761 aaccttggac tatctttttc atcaggctca catccaagca gtagcagaaa tcgttcagcc
     8821 tattcatcag cagttgatgg tggttccagg ccatactaag attgaaaaga ttttttcaca
     8881 tccacaggcc ttggctcaag gaaagaaatt catcgatgaa caatatccag aggctcaaat
     8941 cgaggtaaca gctagtacag cttatgcggc ccgttttatt tccgaacatc cagaccagcc
     9001 ctttgcagca gttgcaccta gaagttctgc tgaagaatat ggattggaac tgattgccga
     9061 ggatattcag gaaatggaag ccaatttcac acgtttctgg gttctaggag ctgaaaagcc
     9121 tagtattccc ttgcaagcac aaactgaaaa gatgagtttg gccttgacat tacctgacaa
     9181 ccttccaggt gcactttata aggccctgtc gacctttgct tggcgaggaa ttgacttgac
     9241 aaaaattgaa agtcgtccac tcaagacagc actgggtgaa tactttttca ttatcgatgt
     9301 ggattatacc gataaggact tggtccactt tgcccaaaaa gaattagaag cgattggaat
     9361 ccagtataaa attctgggtg cctatcctat ttatccaata tcagaccatg gaaaggagag
     9421 aagatgagta aagaaaatcc cttaagtcat catgagcagt tgcgttatga ttatttgcta
     9481 aaaaatattc actatctcaa tgagagagaa aaaaatgagt ttgcctattt gcaagaaaag
     9541 ctaactcttg ctaggggaaa tagtagctct agcttggaac aagaaagaga agagcaggtt
     9601 gacttaccaa gctatgcgaa ccggagtcgc tcacaatcca aatcacaagc actctctttc
     9661 cctccaaaaa agaaaagacg gaagctccgt cttaaacgaa tttttatggt gattttttca
     9721 cttttggtct gtgtggcttt ggccatggta ttcatgtttt tgcgtggtta ccaagatgct
     9781 agcgcaaaga aaactgctga tgcccgggca gctcaagtag aagtctttaa tggtcaggac
     9841 actagagatg gagttaatat tttaatcatg ggtactgatg gtcgaatcgg ccagaacagt
     9901 gttgagacac gaactgactc tattatggta ttaaatgtcg ggggctcaga taagaaaatg
     9961 aagctagtca gtttcatgcg tgataatttg gtctatatag atggttatag tcaagtgatt
    10021 aacggtagaa aacagacaga taacaagtta aacgtagcct acgagttagg agaacaagag
    10081 gggcaaaaag gggcagaaat ggttcgccaa gtcttgaaag ataattttga cttggacatt
    10141 aagtactacg ccttggttga tttccaggcc tttgccacag cgattgacac gcttttccct
    10201 gatggggtga cgattgatgc tcaattttca acattgaatg ggcgtcccct aacagaagct
    10261 acagtcggag atgatttata cgctaccgag actgagtctc caacccaaac cattaaagtc
    10321 ggaaaacagc agatgaatgg ctcgaccctg ctcaattatg ctcgtttccg tgatgatgat
    10381 gaggcggatt acggccgtac aaaaagacag caacaagttt taacagcaat tttagagcaa
    10441 attaaagatc ccactaaact tttcactggt tctgaagctc ttggaaaggt ttttgctatg
    10501 acctcaacca acgtacccta tactttcctc ctaacaaacg gtttatctgt tttggatgga
    10561 gcaaaaaatg gtattgaaaa attgacgatt ccagaactgg gtgactgggt agatgcctat
    10621 gatgtttatg gaggcttggg cttgctggtt gatcaaaaca aatatcaaac caagctcgct
    10681 caaatgggct tgagataaga taatactcaa tgaaaatcaa agtgcaaact aggaagctag
    10741 ccgcaggctg ctaaaaacac agttttgagg ttgtagataa gactgacgaa gtcagttacc
    10801 tatacctacg gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga gtataagaca
    10861 gaaaaatcaa agtttgatag ctatttggat agcagtcagg ctttgatttt ttacagtctg
    10921 caatagattt tcaccccttg tttgtggtat aatgaaacga tacgatagaa agaataatga
    10981 acaatatgac tgatttaaaa gcaattcagg ctcgtagtct ggagatggct gaatattttg
    11041 tggccttttg taaagaacat gatttgctct gttatctctg tggcggaggc gctattggtg
    11101 cccttcgaaa caagggattt attccttggg acgacgacct agactttttt atgcctcgta
    11161 aagattatga gaaattagca gaattatggc ctcgttatgc agatgaacgt tatttcttgt
    11221 caaagagtca caaggatttt gttgatcgta atctttttat taccattcgt gacaaggaaa
    11281 ccacctgtat caagccttat cagcaggatt tggatttgcc acatggtctg gccttggatg
    11341 ttttgccttt ggattattat ccgaaaaatc cagctgagcg gaaaaaacag gttcgttggg
    11401 ccttgattta ttcactcttt tgtgcgcaaa ctattccaga aaagcatggt gctctcatga
    11461 aatggggaag tcgcatttta ctgggtttga ctccaaaatc tctctgttat cgcatctgga
    11521 aaaaagctga gaaagaaatg actaagtatg atttggctga ttgtgatggc attacagaat
    11581 tatgctcagg tcctggctac atgagaaaca agtacccaat cacatctttt gaagacaatc
    11641 ttttcttgcc atttgaagga acagagatgc ctattccaat cggctatgat gtctatctca
    11701 gaactgcttt tggggattat atgacgcctc caccagcaga caagcaggta ccgcatcatg
    11761 atgctgtcat cgctgatatg gataagtctt atacagaata caagggagaa tatggtggct
    11821 aagaaaaaaa tcttattttt tatgtggtct ttttctcttg gaggtggtgc agagaagatt
    11881 ctatcaacca ttgtttcaaa tctggatcca gaaaagtatg atattgatat tcttgaaatg
    11941 gagcactttg acaagggata tgaatctgtt ccaaagcatg tacgcatttt aaaatccctt
    12001 caagattatc gccaaaccag atggttacga gcttttttgt ggagaatgag aatttatttt
    12061 ccaagactgg ctcgtcgttt gcttgtaaaa gatgattatg atgttgaagt ttcttttacc
    12121 attatgaatc caccactgtt gttctctaaa agaagagaag tcaagaagat atcttggatt
    12181 catggaagta ttgaagaact tcttaaggat agctctaaaa gagaatcaca tagaagccag
    12241 ttggatgctg cgaatacaat tgtagggatt tcaaaaaaga ccagcaattc tatcaaggaa
    12301 gtttatccag attatacttc taaattacag acaatctaca atggatatga ttttcagact
    12361 attctagaaa aatctcaaga gaagatcgat atcgagattg ctcctcaaag tatctgtact
    12421 atcggacgga ttgaggaaaa taagggttct gaccgtgtag tggaagtgat acgattatta
    12481 caccaagagg gaaaaaacta tcatctctat tttatcgggg ctggtgatat ggaagaggaa
    12541 ctgaaaaaac gagtcaaaga gtatgggatt gaggactatg tacatttcct tggttatcaa
    12601 aaaaatcctt atcagtatct atctcagacg aaagttcttt tgtctatgtc taaacaagaa
    12661 ggttttcctg gagtgtatgt ggaggccttg agtctgggac tcccttttat ctctacgaac
    12721 gttggagggg ctgaggaatt atcccaagaa ggacgatttg gacaaatcat tgagagcaat
    12781 caagaggcag ctcaggcgat tactaattac atgacttctg cctcaaactt tgatgtcgat
    12841 gaggctagcc aattcattca acaatttaca attacaaaac aaatcgaaca agtagaaaaa
    12901 ctattagagg agtagcatgg aaactgcatt aattagtgtg attgtgccag tctataatgt
    12961 ggcgcagtac ctagaaaaat cgatagcttc cattcagaag cagacctatc aaaatctgga
    13021 aattattctt gttgatgatg gtgcaacaga tgaaagtggt cgcttgtgtg attcaatcgc
    13081 tgaacaagat gacagggtgt cagtgcttca taaaaagaac gaaggattgt cgcaagcacg
    13141 aaatgatggg atgaagcagg ctcacgggga ttatctgatt tttattgact cagatgatta
    13201 tatccatcca gaaatgattc agagcttata tgagcaatta gttcaagaag atgcggatgt
    13261 ttcaagctgt ggtgtcatga atgtctatgc taatgatgaa agcccacagt cagccaatca
    13321 ggatgactat tttgtctgtg attctcaaac atttctaaag gaatacctca taggtgaaaa
    13381 aatacctggg acgatttgca ataagctaat caagagacag attgcaactg ccctatcctt
    13441 tcctaagggg ttgatttacg aagatgccta ttaccatttt gatttaatca agttggccaa
    13501 gaagtatgtg gttaatacta aaccctatta ttactatttc catagagggg atagtattac
    13561 gaccaaaccc tatgcagaga aggatttagc ctatattgat atctaccaaa agttttataa
    13621 tgaagttgtg aaaaactatc ctgacttgaa agaggtcgct tttttcagat tggcctatgc
    13681 ccacttcttt attctggata agatgttgct agatgatcag tataaacagt ttgaagccta
    13741 ttctcagatt catcgttttt taaaaggcca tgcctttgct atttctagga atccaatttt
    13801 ccgtaagggg agaagaatta gtgctttggc cctattcata aatatttcct tatatcgatt
    13861 cttattactg aaaaatattg aaaaatctaa aaaattacat tagaacgggg gtgaccaagt
    13921 gattgatggg aaacgattat tatttagttt gaccatagtc agttatgcct tgacgctagt
    13981 aagtggaatt gtgtatctgt ttaataataa taatgttagc ttactttcta ctttattgtt
    14041 cttgttggtt agtagcttaa ttgcttgttg gaatgatatc aagtattact taatccattt
    14101 tattttctat ttaaccattt ttgtatttct ggtatcaaga ccgaccattg attattttag
    14161 ggatggtgct ttggatacct atcatccaat agcctatcgt tttgccttta tagttgtcat
    14221 ggtttcgatt ctgggcttga ccacaggagg cattctggct cgttacttca tagctaggaa
    14281 gaaaataaaa gtagcaaata taggaaattc tctaaaagag gtttatatca agcggttacg
    14341 ctttgtatca ctaggagttt ttcttctaac ctatcctttc tatttcattc ggttatttga
    14401 acggctcttg tatcgtttgc agacttccta ctatgcctac tatgcaaatt ttgaaagtaa
    14461 actgccttat tttacctaca ttttgtctac ctttacggtc tatgcaatgt gtatgtatct
    14521 ggcaaccaag ccaaagaaat tgcaggccac agcagtgctt gtctccttta ttgcagctaa
    14581 tactattcat ttggcaattg ggacacgaaa tccctttatt ttaagtattt tatttgcttt
    14641 tgtttattac tttatgcggg agcaaactga aaaaggaaaa tggattgggt ttaaagaaaa
    14701 gttagcgatt tttgtaggtt ctcctattct catgttagcg atgggagtac tcaattatgt
    14761 acgggataat gtccaagttt ctcatacagg tttctgggat atcttacttg actttatcta
    14821 taaacaaggg actagttttg gtgttctggc tcgaggtttt ctatttaaca gtagcctccc
    14881 ttaccgagat ttccgtaatt ttacttttgg tcctgttctt gattattttg caagggggag
    14941 tttgggagcc attttcggag gaaaagcctt tgaacataca accaatagtg tggaactagc
    15001 tattgatagt aatagttatg cccacaatct atcctatctt gtcttgaaca aggaatactt
    15061 gaaagggcat ggtatcggaa gtagttatat catggagttg tataccgact atggtatgat
    15121 tggagtcttt ctgcttagtt tcttactcgg cgtattattt atagccatgc tgcaagtagc
    15181 ctatcgctca aggacaatcc tatttgcttt atccctactc atcttgaata atctattctt
    15241 tatgccaaga agcagctttt cagaaagttt cttcaattta tttacaatgc aattctgggg
    15301 aattgttctt gtgattatat ttgtagcaaa aatgcttaca aaggaaaact agtatctact
    15361 aaacaaagga gaaaaaaatc atgtttgaac attattcagt agctgatttg tttgcaaatc
    15421 tttataaaaa gcgaaaagca aatattttag ctctcatcgc tttatttgct ctcattgctg
    15481 taccatttac aattaaagca gttaggaata aaaacactgt caaagataca acaagttatt
    15541 caacttatct tatctataaa atcactcctc caaaagagtc ggccaaaacg attttgaatc
    15601 atcaaattgg tggttatagt gatttttatg ggaaattgat tgatggtaat ttgaatggag
    15661 cttatctttt caatgatgta gaacccagtg agttgaaaaa aattgccagt gaattagaca
    15721 cgacagaaac aaccttgaaa aattcaacga atgactattg gtggaaaaaa ttgaccgtct
    15781 actatatgat tgacgatgca ggggttggtg tgaaaatttt gacatcaagt aaagatgcca
    15841 atgacttgtt agagaaaaaa attgatgggt tgattgagaa atttaaacat gcctatgcaa
    15901 atgtgaaaat tgaaaaactg gaaaccatca actctaaaga attgaacgca aatggtgaaa
    15961 cagcgcttgg cttaaatgtg aaaaatctga ttcttcgttt agttgttatt ggagtggttt
    16021 gtgtgatttt ggttgtgatg ggaaatgtgt tagtttatct ctttaatcca acaatcaata
    16081 gagtaggtga tttttctcag tatcaaattg attttgtaac agagattaca acaattgcta
    16141 acctagcaga tgttttgtca tacaaaaata ctggacagga attgaccatc gttagctcaa
    16201 ataaagctat cctagataaa ttgaaacaga gtcaagaagc tttaaaagga atgcattttg
    16261 tagatttaca ggatgtatca tctcttttgg aaagagatac agtccttctt gttgaagagt
    16321 acggagtgac tcgttataag aaatttgagc aaagtcttca aattctcaga aacttaaatc
    16381 gttctatcct tggtgtagca acctttaaat tataagtatt ctgatgtata aggtcttcaa
    16441 aatcggctaa gagctgattt tgaaggcttt ttctatctgt ctgcagaaat tttcttttgg
    16501 agtctcctat agaaagaccc tagggaaaat ctatccgtat agaggagcct tcttttgata
    16561 aaatcgtaaa aatctagtat aatagataga actgaaagta tgaggttact agcaatgaaa
    16621 atgaaacaaa ttagtgatac aactttaaaa atcacgatgt ctttagagga tttgatggat
    16681 cgtggaatgg agattgctga ctttctcgtt cctcaagaaa aaacagaaga gttcttttat
    16741 gctatcttgg atgaactaga gatgcctgat agctttctgg atacaggcat gttgagcttc
    16801 cgtgtgactc caaaacctga taaggtagat gtctttgtga ccaagtcaaa gattgaccaa
    16861 aatctagatt ttgaagactt atcggatttg ccagatatgg aagaattggc tcaaatgtct
    16921 ccagatgaat ttatcaaaac cctggaaaaa agcatcgcag acaaaaccaa ggatgatatc
    16981 gaagccattc aatctcttga gcaagttgaa gccaaggaag aagagcaaga gcaggctgaa
    17041 caagaagctg agagtaagaa agaaccttac atctactaca tcctttcttt tgctaagttg
    17101 gctgacttgg tagtttttgc caagacagtg acttttgaaa tggaaacttc tgaactctac
    17161 aaaatgaacg agcgctatta tttgaccatt ttagtggata ttgaaaatca tccaagccca
    17221 tatccagctt ggctgttggc ccgtatgcgc gagtttgcag acgatagtga tatcagtcgc
    17281 tcagtcttac aagagtatgg tcaagtcttg atgagtcacg atgcagtgct caatctgcaa
    17341 aaaatcggct aatcctttct ggaaagctat tttcagattt taagaagagc gaatcgtctg
    17401 attcgttttt tctttttata ctgaaatagt gatttactat aataggaatt ttcacaaaat
    17461 tctgttataa tggctatatt agaaaatttc gaggagataa agatgacagt taaaattgct
    17521 ttactaggat ttggtaccgt tgcaagtggt gtgcctttcc tcctaaagga aaatggagga
    17581 aaaatcaatc aatcagcaca ttcagatatc aaagttgcta aggtattggt caaggatgaa
    17641 gatgaaaaaa atcgcttgct tgcagcaggg aatgacttta actttgtaac caatgtggat
    17701 gatattttat cagaccagga tattactatc gtagtggaat tgatggggcg tattgagcct
    17761 gctaaaacct ttatcactcg tgccttggaa gctggaaaac acgttgttac tgctaacaag
    17821 gaccttttag ctgtccatgg cgcagaattg ctagaaatcg ctcaagctaa caaggtagca
    17881 ctttactacg aagcagcagt tgctggtggg attccaattc ttcgtacttt agcaaattcc
    17941 ttggcttctg ataaaattac gcgcgtgctt ggagtagtca acggaacttc caacttcatg
    18001 gtgaccaaga tggtggaaga aggctggtct tacgatgatg ctcttgcgga agcacaacgt
    18061 ctaggatttg cagaaagcga tccgacgaat gacgtagatg ggattgatgc agcctacaag
    18121 atggttattt tgagtcaatt tgcctttggc atgaagattg cctttgatga tgtagcccac
    18181 aagggaatcc gcaatatcac accagaagac gtagctgtag ctcaagagct tggttacgta
    18241 gtgaaattgg ttggttctat tgaggaaact tcttcaggta ttgctgcaga agtgactcca
    18301 accttcctac ctaaagcgca cccacttgct agtgtgaatg gcgtaatgaa cgctgtcttt
    18361 gtagaatcta ttggtattgg tgagtctatg tactacggac caggtgcggg tcaaaaacca
    18421 actgcaacaa gtgttgtagc tgatattgtc cgtatcgttc gtcgtttgaa tgatggtact
    18481 attggcaaag acttcaacga atatagccgt gacttggtct tggcaaatcc tgaagatgtc
    18541 aaagcaaact actatttctc aatcttggct ctagactcaa aaggtcaggt cttgaagttg
    18601 gctgaaatct tcaatgctca agatatttcc tttaagcaaa tccttcaaga tggcaaagag
    18661 ggtgacaagg cgcgtgtcgt tatcatcaca cacaagatta ataaagccca gcttgaaaat
    18721 gtctcagctg aattgaagaa ggtttcagaa ttcgacctct tgaatacctt caaggtgcta
    18781 ggagaataag atgaagatta ttgtacctgc aaccagtgcc aatatcgggc caggttttga
    18841 ctcggtcggt gtagctgtaa ccaagtatct tcaaattgag gtctgcgaag aacgagatga
    18901 gtggctgatt gaacaccaga ttggcaaatg gattccacat gatgagcgta atctcttgct
    18961 caaaatcgct ttgcaaattg taccagactt gcaaccaaga cgcttgaaaa tgaccagtga
    19021 tgtccctttg gcgcgcggtt tgggttcttc cagctcggtt atcgttgctg ggattgaact
    19081 agccaaccaa ctgggtcaac tcaacttatc agaccatgaa aaattgcagt tagcgaccaa
    19141 gattgaaggg catcctgaca atgtggctcc agccatttat ggtaatctcg ttattgcaag
    19201 ttctgttgaa gggcaagtct ctgctatcgt agcagacttt ccagagtgtg attttctagc
    19261 ttacattcca aactatgaat tacgtactcg cgacagccgt agtgtcttgc ctaaaaaatt
    19321 gtcttataag gaagctgttg ctgcaagttc tatcgccaat gtagcggttg ctgccttgtt
    19381 ggcaggagac atggtgaccg ctgggcaagc aatcgaggga gacctcttcc atgagcgcta
    19441 tcgtcaggac ttggtaagag aatttgcgat gattaagcaa gtgaccaaag aaaatggggc
    19501 ctatgcaacc tacctttctg gtgctgggcc gacagttatg gttctggctt ctcatgacaa
    19561 gatgccaaca attaaggcag aattggaaaa gcaacctttc aaaggaaaac tgcatgactt
    19621 gagagttgat acccaaggtg tccgtgtaga agcaaaataa agaatagaag ataggatggg
    19681 gaaactcttg accagagggt ttcatatcct ttttgtgaaa agaagtttat actcaatgaa
    19741 aatcaaagag caaactagga agctagccgc aggctgctca aaacagtgtt ttgaggttgt
    19801 ggatagaact gacgaagtca gctcaagaca ctgttttgag gttgcagata gaactgacga
    19861 cgtcagtaac atatatacgg taaggtgacg ctaacgtggt ttgaagagat tttcgaagag
    19921 tattagttaa aaacgtgata aaggagaaat aaagatggca gaaatttatc tagcaggtgg
    19981 ttgtttttgg ggcctagagg aatatttttc acgcatttct ggagtgctag aaaccagtgt
    20041 tggctacgct aatggtcaag tcgaaacgac caattaccag ttgatcaagg aaacagacca
    20101 tgcagaaacg gtccaagtga tttacgatga gaaggaagtg tcactcagag agattttact
    20161 ttattatttc cgagttatcg atcctctatc tatcaatcaa caagggaatg accgtggccg
    20221 ccaatatcga actgggattt attatcagga tgaagcagat ttgccagcta tctacacagt
    20281 ggtgcaggag caggaacgca tgctgggtcg aaagattgca gtagaagtgg agcaattacg
    20341 ccactacatt ctggctgaag actaccacca agactatctc aggaagaatc cttcaggtta
    20401 ctgtcatatc gatgtgaccg atgctgataa gccattgatt gatgcagcaa actatgaaaa
    20461 gcctagtcaa gaggtgttga aggccagtct atctgaagag tcttatcgtg tcacacaaga
    20521 agctgctaca gaggctccat ttaccaatgc ctatgaccaa acctttgaag aggggattta
    20581 tgtagatatt acgacaggtg agccactctt ttttgccaag gataagtttg cttcaggttg
    20641 tggttggcca agttttagcc gtccgatttc caaagagttg attcattatt acaaggatct
    20701 gagccatgga atggagcgaa ttgaagttcg ttctcgttca ggcagtgctc acttgggtca
    20761 tgttttcaca gatggaccgc gggagttagg cggcctccgt tactgtatca attctgcttc
    20821 tttacgcttt gtggccaagg atgagatgga aaaagcagga tatggctatc tattgcctta
    20881 cttaaacaaa taaaacagag agtggggctt cccactttct tcatttctag aatatgaata
    20941 gaagggattt atgaaacacc tattatctta cttcaaaccc tacatcaagg aatcaatttt
    21001 agcccccttg ttcaagctgt tagaagctgt ttttgagctc ttggttccca tggtgattgc
    21061 tgggattgtt gaccaatctt tacctcaggg agatcaaggt catctctgga tgcagattgg
    21121 cctgctcctt atctttgcag taattggcgt tttagtggcc ttgatagctc aattttactc
    21181 agcaaaggca gcagtaggtt ttgctaagga attgacaaac gatctttatc gtcatattct
    21241 ttccttgcca aaggacagca gagaccgtct gacaacttct agtttggtta ctcgcttgac
    21301 ttcggatacc taccagattc agactggtat caatcaattc ctgcgtctct ttttacgagc
    21361 gcccattatc gtttttggtg ccatttttat ggcttatcga atctcagctg agttgacttt
    21421 ctggttctta gtcttggttg ccattttgac cattgtcatt gtagggttat ctcgattggt
    21481 caatcctttc tacagtagtc tcagaaagaa aacggaccaa ctggttcagg aaacgcgcca
    21541 gcaattgcaa gggatgcggg ttattcgtgc ttttggtcaa gaaaaacgag agttacagat
    21601 ttttcaaacc cttaaccaag tttatgctag attacaagaa aagacaggtt tctggtctag
    21661 tttattaaca cctctgacct atctgattgt caatggaact cttctcgtta ttatctggca
    21721 aggctatatt tcaattcaag gaggagtgct cagtcaaggt gctctcattg ctcttatcaa
    21781 ttacctctta cagattttgg tggaattggt caagctagcc atgttgatca attccctcaa
    21841 ccagtcctat atctcagtca agcgaatcga ggaagtcttt gttgaggctc cagaggatat
    21901 ccattcagag ttagaacaaa agcaagctac cagagataag gttttacaag tccaagaatt
    21961 gacttttacc tatcctgatg cggcccagcc ttctctgaga tacatttcct ttgatatgac
    22021 tcaaggacaa attctaggta tcatcggggg aactggttct ggtaaatcaa gcttggtgca
    22081 actcttactt ggactttatc cagtagacaa ggggaacatt gacctttatc aaaatggacg
    22141 tagtcctctt aatttggagc agtggcggtc ttggattgcc tatgtacctc aaaaggtcga
    22201 actctttaaa ggaaccattc gttccaactt gactctaggt ttcaatcaag aagtatctga
    22261 ccaggaactc tggcaggcct tggagattgc gcaagctaag gattttgtca gtgaaaagga
    22321 aggactcttg gatgctctag ttgaggcagg tgggcgaaat ttctcaggtg gacaaaaaca
    22381 aagattgtct atcgcccgag cagtcttgcg ccaagctccg tttctcatcc tagatgatgc
    22441 aacctcggca ctggatacca ttacagagtc caagctcttg aaagctatta gagaaaattt
    22501 tccaaacacg agcttaattt tgatctctca acgaacctca actttacaga tggcggacca
    22561 gattctcctc ttggaaaaag gtgagttgct agctgttggc aagcacgatg acttgatgaa
    22621 atccagccaa gtctattgtg aaatcaatgc atcccaacat ggaaaggagg actagaatga
    22681 aacgacaaac tgtaaaccag acgctcaaac gtttagccgt agatttagca agccatcctt
    22741 tcctcctttt cctagccttt ctaggaacta ttgctcaagt tggcttatca atttacctac
    22801 ctattctgat tgggcaggtc attgaccaag tcctagtggc tggttcatca ccagtttttt
    22861 ggcagatttt tctccagatg ctcttggtgg taataggaaa tactctggta caatgggcca
    22921 atcctctcct ctataatcgt ctaatcttct cttataccag agatttacgg gagcgaatca
    22981 tccataagct ccatcgttta ccgattgcct ttgtagatag gcaaggtagt ggagagatgg
    23041 ttagtcgtgt aaccacggac atcgaacagt tggcagctgg cttgaccatg atttttaacc
    23101 aatttttcat tggtgttttg atgattttgg tcagtattct agccatgctc caaattcatc
    23161 tcctcatgac tctcttagtc ttgctgttga cgccactgtc catggtgatt tcacgcttta
    23221 ttgccaagaa atcctatcat ctcttccaga agcaaacaga gacgagggga attcagactc
    23281 agttgattga agaatcgctt agtcagcaga ctataatcca gtccttcaat gctcaaacag
    23341 aatttatcca aagattgcgt gaggctcatg acaactactc aggctattct cagtcagcca
    23401 tcttttattc ttcaacggtc aatccttcga ctcgctttgt aaatgcactc atttatgccc
    23461 ttttagctgg agtaggagct tatcgtatca tgatgggttc agccttgacc gtcggtcgtt
    23521 tagtgacttt tttgaactat gttcagcaat acaccaagcc ctttaacgat atttcttcag
    23581 tgctagctga gttgcaaagt gctctggctt gcgtagagcg tatctatgga gtcttagata
    23641 gccctgaagt ggctgaaaca ggtaaggaag tcttgacgac cagtgaccaa gttaagggag
    23701 ctatttcctt taaacatgtc tcttttggct accatcctga aaaaattttg attaaggact
    23761 tgtctatcga tattccagct ggtagtaagg tagccatcgt tggtccgaca ggtgctggaa
    23821 aatcaactct tatcaatctc cttatgcgtt tttatcccat tagctcggga gatatcttgc
    23881 tggatgggca atccatttat gattatacac gagtatcatt gagacagcag tttggtatgg
    23941 tgcttcaaga aacctggctc acacaaggga ccattcatga taatattgcc tttggcaatc
    24001 ctgaagccag tcgagagcaa gtaattgctg ctgccaaagc agctaatgca gactttttca
    24061 tccaacagtt gccacaggga tacgatacca agttggaaaa tgctggagaa tctctctctg
    24121 tcggccaagc tcagctcttg accatagccc gagtctttct ggctattcca aagattctta
    24181 tcttagacga ggcaacttct tccattgata cacggacaga agtgctggta caggatgcct
    24241 ttgcaaaact catgaagggc cgcacaagtt tcatcattgc tcaccgtttg tcaaccattc
    24301 aggatgcgga tttaattctt gtcttagtag atggtgatat tgttgaatat ggtaaccatc
    24361 aagaactcat ggatagaaag ggtaagtatt accaaatgca aaaagctgcg gcttttagtt
    24421 ctgaataagc cattctcttt tgaaagttta tggacgaaaa aagttgcctt cgagtgactt
    24481 ttttgttaca atagctagaa aaattgttca ctgtaatact caatgaaaat caaagagcaa
    24541 actaggaagc tagccgtagg ttgctcaaag cacagctttg aggttgtaga taagactgac
    24601 gaagtcagtt caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa
    24661 cactgttttg aggttgcaga tagaactgac gaagtcagct caaaacactg ttttgaggtt
    24721 gcagatagaa ctgacgaagt cagtaaccac acctacggca aagtgaatct gaagtggttt
    24781 gaagagagta caacttgtct tttagaaaag gagcctataa tgaaagtctt tcagcatgta
    24841 aatatcgtga cttgtgatca agatttccat gtttatcttg atggaatctt agcagtcaag
    24901 gattctcaaa tcgtctatgt cggtcaagat aagccagcgt ttttagagca agctgagcag
    24961 attatagact atcagggagc ttggattatg cctggtttgg tcaattgtca catccattct
    25021 gcaatgacag gtctgagagg gatccgagat gacagcaatc tccatgaatg gctcaatgac
    25081 tatatttggc cagcagaatc tgagtttact cccgacatga ctaccaatgc ggtcaaagaa
    25141 gccctgacag agatgctcca gtcaggaaca acaaccttta acgatatgta taatcccaat
    25201 ggtgtggata tccagcaaat ttatcaggtg gtgaaaactt ccaagatgcg ttgttatttt
    25261 tctccgactc tcttttcttc agagacagaa acaactgctg agactataag tagaactcga
    25321 tccatcatag acgaaatctt aaaatataaa aatccaaatt tcaaggttat ggtagcacct
    25381 cattctccgt atagctgcag tagagacttg ctggaagcga gtttggaaat ggcaaaagag
    25441 ctaaatattc cgctccatgt ccatgtggcg gagaccaagg aggagtcagg aattatcctc
    25501 aaacgatacg gcaaacgccc ccttgctttt cttgaagaac tgggttattt agatcatccg
    25561 tccgtatttg ctcacggggt cgaattaaac gagagagaaa ttgaacgctt ggcatcttct
    25621 caagtggcta tcgcccacaa tcctatcagt aacctcaaac tggcatcagg aattgctcca
    25681 attatccagc tccaaaaagc gggagtagca gtcggaattg cgactgactc ggttgcttcc
    25741 aataacaatc tagatatgtt tgaggaagga aggactgcag ctcttcttca gaagatgaaa
    25801 agtggggatg ccagccagtt tccaatcgaa acagctctca aggtactgac aatcgaaggg
    25861 gctaaggtcc ttggaatgga aaatcagata ggaagtctgg aagtcggcaa gcaagcagat
    25921 tttctggtca ttcaaccaca agggaaaatt catctccaac ctcaggaaaa tatgctgtct
    25981 cacctggttt atgcagttaa atctagtgat gtagatgatg tttatatcgc cggagaacag
    26041 gttgttaagc aaggtcaagt cctgacagta gaactttaaa agaaaaatta cgaaaaattt
    26101 taaaaaaagt ttgcaaaaat cttgcattct ttttttgact atgctatact tatatacggt
    26161 ttaaaaaaac tgcctaagac agtaggggag ctcgactcat aaatatccta ccgaggacaa
    26221 aacgtatcat gtaaaaagaa gcgtattgta ctttcgtgtc taggtttggg cgcgtttttc
    26281 tttttgaaaa attccccaag caaaataatt acggaggtga acacactaat gagtgaagca
    26341 attattgcta aaaaagcgga actagttgac gtagtagctg aaaaaatgaa agctgctgca
    26401 tctatcgtcg ttgtagacgc tcgtggtttg acagttgagc aagatacagt tcttcgtcgt
    26461 gagcttcgtg gaagcgaagt tgagtataaa gttattaaaa actcaatctt gcgtcgtgca
    26521 gctgaaaaag ctggtcttga agatcttgca tctgtatttg ttggaccatc tgcagtagca
    26581 ttttctaatg aagatgttat cgcaccagcg aaaatcttga acgacttttc taaaaacgct
    26641 gaagcacttg aaattaaagg tggtgcaatc gaaggcgctg tcgcatctaa agaagagatt
    26701 cttgcacttg caactcttcc aaaccgcgaa ggacttcttt ctatgctcct ttctgtactt
    26761 caagcgccag tgcgcaacgt tgctcttgca gtcaaagcgg ttgcagaaag caaagaagac
    26821 gcggcttaat cttaagctat acagcgtagc ctagctacga aaaaaactat tataaaattt
    26881 aaaacttatt tggaggaaat aacaatggca ttgaacattg aaaacattat tgctgaaatt
    26941 aaagaagctt caatccttga attgaacgac cttgtaaaag ctatcgaaga agaatttggt
    27001 gtaactgcag ctgctcctgt agctgttgct gcagctgatg cagctgatgc tggtgctgct
    27061 aaagattcat tcgacgttga attgacatct gcaggcgaca aaaaagttgg cgttatcaaa
    27121 gttgtacgtg aaatcactgg tcttggtctt aaagaagcta aagaacttgt tgacggtgca
    27181 ccagcacttg ttaaagaagg cgttgcaact gcagaagctg aagaaatcaa agctaaattg
    27241 gaagaagctg gagcttcagt tactcttaaa taatagagta tcgcaatttg attgcggaat
    27301 ccctatttac cagtctttga gagcgataag cgattgatag aaactgataa attgatttgg
    27361 tttcctgcca aaaattcttt ggcaggattt ttgttttaat aaactttcgg gggtagacac
    27421 tagattgtct accccttttt ttgtgttttg gaggtaatta tgcaaaagga aggacagtca
    27481 tcacgtcaat tcgtgaagag tcatctcatg actatcatgg gagttgatat tgtaaaagca
    27541 actcagttag ttgatgaaat ggaacaagta ggtttaatcc gatttgatga atttggaaat
    27601 gttggaattt tagtcttgga gggacaataa tgaagcgtat taccgctaat caatatcaga
    27661 catcagagcg ttattataag ctccccaaac ttttatttga gagtgaacgg tataagaata
    27721 tgaaactgga ggttaaggtg gtttactcag ttttaaaaga tcggttggag ttgtctttga
    27781 gcaaaggttg gattgatgag gatggggcta tttatttgat ttattccaat tcaaatttga
    27841 tggcactttt aggctgttca aagtcaaaat tactctccat gtgagtttga agtgacattt
    27901 ttagatgatt accataaaaa acataactac ccactatttt acgaatccta tcttcaaaac
    27961 gttatggaat tccttgaaag tcaagacata aagaatgggg ttgatgcctt tgtagatgat
    28021 catcaaaatc tcgtttttgt tttatatgga caaggctatc gagccgaggg aaaagaggga
    28081 atacttacaa cccaagtaac tgtaaaagct tatgatgaag acaagaaacc gattaacttc
    28141 gcaaatttat tagattcctt aatttattag attccttaat cgtgtcagaa tatcaaatgg
    28201 aaccgaatct ttgggaggtc tcccatgatt gatctctatc taagtaaaaa tagacaaaga
    28261 aatcaacttc ttttagattt cttccaaaac tatggcatag aggtatcttg tcattcagtt
    28321 tctgaaatga caaaggacaa attaattgag atgatgagct attcttcaga ttgttttgag
    28381 tttttatctc ccaatttatt acgatttaag aaccgtgaca atctaagatt aacggatttc
    28441 gttgagataa ttttaaaaaa tcctgaacta accatcagac ttcctcttgc ggtttcaaat
    28501 aagcgagttt atccaagtct gaatttggaa gaggctagag ctctattgcc aagagatacg
    28561 aaacaattga tttacatggc gcaaacacat tatttatcta actaaaggag aagcaatatg
    28621 gctgaacgat
//
LOCUS       DAYYQN010000025        26777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYYQN010000025 DAYYQN010000000
VERSION     DAYYQN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.025"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            269..682
                     /gene="rpsL"
                     /locus_tag="ACHBRQ_001005"
     CDS             269..682
                     /gene="rpsL"
                     /locus_tag="ACHBRQ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9592695.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            702..1172
                     /gene="rpsG"
                     /locus_tag="ACHBRQ_001006"
     CDS             702..1172
                     /gene="rpsG"
                     /locus_tag="ACHBRQ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9592696.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1597..3678
                     /gene="fusA"
                     /locus_tag="ACHBRQ_001007"
     CDS             1597..3678
                     /gene="fusA"
                     /locus_tag="ACHBRQ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9592697.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3782..8173
                     /locus_tag="ACHBRQ_001008"
     CDS             3782..8173
                     /locus_tag="ACHBRQ_001008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9592698.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8275..8538
                     /locus_tag="ACHBRQ_001009"
     CDS             8275..8538
                     /locus_tag="ACHBRQ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9592699.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8531..8809
                     /locus_tag="ACHBRQ_001010"
     CDS             8531..8809
                     /locus_tag="ACHBRQ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HFM9592700.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8830..8982
                     /locus_tag="ACHBRQ_001011"
     CDS             8830..8982
                     /locus_tag="ACHBRQ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592701.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            9004..10245
                     /locus_tag="ACHBRQ_001012"
     CDS             9004..10245
                     /locus_tag="ACHBRQ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9592702.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10255..10485
                     /locus_tag="ACHBRQ_001013"
     CDS             10255..10485
                     /locus_tag="ACHBRQ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9592703.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            10753..11475
                     /locus_tag="ACHBRQ_001014"
     CDS             10753..11475
                     /locus_tag="ACHBRQ_001014"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9592704.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11692..13026
                     /gene="pepC"
                     /locus_tag="ACHBRQ_001015"
     CDS             11692..13026
                     /gene="pepC"
                     /locus_tag="ACHBRQ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HFM9592705.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(13082..13993)
                     /locus_tag="ACHBRQ_001016"
     CDS             complement(13082..13993)
                     /locus_tag="ACHBRQ_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9592706.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(14017..14820)
                     /locus_tag="ACHBRQ_001017"
     CDS             complement(14017..14820)
                     /locus_tag="ACHBRQ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9592707.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14848..15837)
                     /locus_tag="ACHBRQ_001018"
     CDS             complement(14848..15837)
                     /locus_tag="ACHBRQ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9592708.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(16071..17090)
                     /gene="adhP"
                     /locus_tag="ACHBRQ_001019"
     CDS             complement(16071..17090)
                     /gene="adhP"
                     /locus_tag="ACHBRQ_001019"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFM9592709.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKGKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(17429..18241)
                     /locus_tag="ACHBRQ_001020"
     CDS             complement(17429..18241)
                     /locus_tag="ACHBRQ_001020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9592710.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKETIKKVAEKGHQIIITTG
                     RPYRMSKDFYHELGLNTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVRRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPENQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            18431..19849
                     /locus_tag="ACHBRQ_001021"
     CDS             18431..19849
                     /locus_tag="ACHBRQ_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9592711.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGILL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFKDLKTIFGAALGSEGLGALISDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTSIGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLASLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQAK
                     DVHVMIWILDALFILNYISMAL"
     gene            19954..20661
                     /locus_tag="ACHBRQ_001022"
     CDS             19954..20661
                     /locus_tag="ACHBRQ_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9592712.1"
                     /translation="MIKKNIWDEVLNRGKWLIIFLVGLLFSQFPLALATFLTSRKFPL
                     LQTGLLVGALSLVVLTVFIIGARKTQLASFGLSFFKAKDLARLALSYLVILAFNILGV
                     VLLHLMNETTTSNQSNINDLVQNSSLISSFFLLLLIAPICEEILCRGVIPKKLFRGKE
                     NVGYIVGAIIFALLHLPTNLPSLLIYGGMSTVLTWTVYKTQRLEMSILLHMIVNGIFF
                     CLLALVVILSRTLGISV"
     gene            20755..21735
                     /gene="folP"
                     /locus_tag="ACHBRQ_001023"
     CDS             20755..21735
                     /gene="folP"
                     /locus_tag="ACHBRQ_001023"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFM9592713.1"
                     /translation="MMKSLEGVTDMSTKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMARVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQAFTEEELADFETLPIEDLMDAFFERALARANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            21737..23059
                     /locus_tag="ACHBRQ_001024"
     CDS             21737..23059
                     /locus_tag="ACHBRQ_001024"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9592714.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     QSLLEGEAVANLQSTTEFEIITALAYDYFAAEQVEVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVSHQKSVVTGEVFDYTSVVRQGRFQTGLLGLHQIENAGMAIALLDTFCQE
                     HGRELASNDLLGQALEETSWPGRLEVVSRDPLMILDGAHNPHAIKALLATLQERFADY
                     RKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFDDSRATDESVLEEAAKSRNLNYQG
                     WQDFLEQKLTDKKEEKKTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            23040..23594
                     /gene="folE"
                     /locus_tag="ACHBRQ_001025"
     CDS             23040..23594
                     /gene="folE"
                     /locus_tag="ACHBRQ_001025"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFM9592715.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMEYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            23753..24565
                     /gene="folK"
                     /locus_tag="ACHBRQ_001026"
     CDS             23753..24565
                     /gene="folK"
                     /locus_tag="ACHBRQ_001026"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFM9592716.1"
                     /translation="MDQLQIKDLEIFAYHGLFPSEKELGQKFVVSAILFYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETTEDLIETVAYKLVERTFEAYPLVQEMKLELKKPWA
                     PVHLPLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNQGIHILKESSVLTT
                     EPWGGVEQDSFANQVVEVETWLPAPVLLESLLAIESEMGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYNALKQ"
     gene            complement(24611..24928)
                     /locus_tag="ACHBRQ_001027"
     CDS             complement(24611..24928)
                     /locus_tag="ACHBRQ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9592717.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            25285..25731
                     /gene="rplM"
                     /locus_tag="ACHBRQ_001028"
     CDS             25285..25731
                     /gene="rplM"
                     /locus_tag="ACHBRQ_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9592718.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            25751..26143
                     /gene="rpsI"
                     /locus_tag="ACHBRQ_001029"
     CDS             25751..26143
                     /gene="rpsI"
                     /locus_tag="ACHBRQ_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9592719.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(26405..>26777)
                     /locus_tag="ACHBRQ_001030"
     CDS             complement(26405..>26777)
                     /locus_tag="ACHBRQ_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9592720.1"
                     /translation="LQLLTSWKKQQKYELAKFGIISNPEQFIFTYIDTKGNINKPLHS
                     DYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLEAISEALIHSDTGTTQIY
                     VNTSNVVPMAFGEFALQSLKQ"
BASE COUNT         7838 a         5221 c         6107 g         7611 t
ORIGIN      
        1 atgtcacata agccaagccg tattattagg aagatgatgt gttcccagct atttttattc
       61 agaaattgat ctctaacatt accaataaaa aagaaaaata tcttgacaag caagggaaaa
      121 ttttgttaca ataatagacg gtacttttta cttttggtct ctcaagagtg tacagggacg
      181 tgctgacaaa tgttgcaaaa gtacacacag atgatatctg tcaccaagtg tatcatcacc
      241 aaaaataaaa aaacacagga gaatgtagat gcctacaatt aaccaattgg ttcgcaaacc
      301 gcgtaaatca aaagtagaaa aatctaaatc accagctttg aacgttggtt acaatagtca
      361 taaaaaagtt caaacaaacg tttcttcacc acaaaaacgt ggtgttgcaa ctcgtgttgg
      421 aacaatgaca cctaaaaaac ctaactcagc ccttcgtaaa ttcgctcgtg tacgtttgag
      481 caaccttatc gaagttactg cctacatccc aggtatcgga cacaacttgc aagagcacag
      541 cgtggtgctt cttcgcggtg gacgtgtaaa agaccttcca ggggtacgtt accatatcgt
      601 ccgtggtgca cttgatactg caggtgttaa cgatcgtaaa caaggccgtt ctaaatacgg
      661 tactaaacgt ccaaaagcat aaggaaaggg gataaagaga aatgagtcgt aaaaatagag
      721 ctccaaaacg tgacgtattg ccagatccgc tatacaattc acaactagtt actcgtctta
      781 tcaaccgcgt tatgcttgat ggtaaacgtg gtactgctgc ttcaatcgtt tacggtgctt
      841 ttgagcaaat caaagaagct actggcaacg atgcacttga agtatttgaa acagctatgg
      901 aaaacatcat gcctgtactt gaagtacgtg cacgtcgtgt tggtggttct aactaccaag
      961 tcccagttga agttcgtcca gaacgtcgta caacacttgg acttcgttgg ttggtaacaa
     1021 tcgctcgtct tcgtggtgaa cacacaatgc aagaccgtct tgcaaaagaa atcttggatg
     1081 ctgctaacaa cactggtgca gcagttaaga aacgtgaaga tactcaccgt atggctgaag
     1141 ctaaccgtgc attcgcacac ttccgttggt aagataggat gcgaaagcgt taagaaagtc
     1201 ccagagaaaa tagggaatcg aagcaggttg cggttgcaac caatgagatt catctttttc
     1261 tccagacttt tagcttgagc tcaactaaat catgatgcta ggcacggtaa ggatgcaagg
     1321 tgaaaatagg aaactgacgc agtattcaac gaatacaagg agttttatct ttttcacgca
     1381 gcatcccgtt ccagctcaca tcggctaact aactttagcc tgggttcaaa ttagctaaat
     1441 cgattagtat tagctataac tcagcttacc atctcgtaag ttgaaaccaa caatagcatg
     1501 aaaacattga gaacgggtag gtcctgccta tccgttttta ttaaaatcgt gttataatag
     1561 aatagaaatc aaaaataaat aggagaaaca aacctcatgg cacgcgaatt ttcacttgaa
     1621 aaaactcgta atatcggtat catggctcac gtcgatgccg gtaaaacaac aactactgag
     1681 cgtattcttt actacactgg taaaatccac aaaatcggtg aaactcacga aggtgcgtca
     1741 caaatggact ggatggagca agagcaagaa cgtggtatca cgatcacatc tgctgcgaca
     1801 acagctcaat ggaacaacca ccgcgtaaac atcatcgaca caccaggaca cgtggacttc
     1861 acaatcgaag tacaacgttc tcttcgtgta ttggatggtg cggttaccgt tcttgactca
     1921 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaactga gtacggagtt
     1981 ccacgtatcg tatttgccaa caaaatggac aaaatcggtg ctgacttcct ttactctgta
     2041 agcacacttc acgatcgtct tcaagcaaat gcacacccaa tccaattgcc aatcggttct
     2101 gaagatgact tccgtggtat cattgacttg atcaagatga aagctgaaat ctatactaac
     2161 gaccttggta cggatatcct tgaagaagac atcccagctg aataccttga ccaagctcaa
     2221 gaataccgtg aaaaattgat tgaagcagtt gctgaaactg acgaagaatt gatgatgaaa
     2281 tacctcgaag gtgaagaaat cactaacgaa gaattgaaag ctggtatccg taaagcgact
     2341 atcaacgttg aattcttccc agtattgtgt ggttcagcct tcaaaaacaa aggtgttcaa
     2401 ttgatgcttg atgcggttat cgactacctt ccaagtccac ttgacatccc agcaatcaaa
     2461 ggtattaacc cagatacaga cgctgaagaa actcgtccag catctgacga agagccattt
     2521 gcagctcttg ccttcaagat catgactgac ccattcgtag gtcgtttgac attcttccgt
     2581 gtttactcag gtgttcttca atcaggttca tacgtattga atacttctaa aggtaaacgt
     2641 gaacgtatcg gacgtatcct tcaaatgcac gctaacagcc gtcaagaaat cgacactgtt
     2701 tactcaggtg atatcgctgc tgccgttggt ttgaaagata ctacaactgg tgactcattg
     2761 acagatgaaa aagctaaaat catccttgag tcaatcaacg ttccagaacc agttatccaa
     2821 ttgatggttg agccaaaatc taaagctgac caagacaaga tgggtatcgc ccttcaaaaa
     2881 ttggctgaag aagatccaac attccgcgtt gaaacaaacg ttgaaactgg tgaaacagtt
     2941 atctcaggta tgggtgaact tcaccttgac gtccttgttg atcgtatgcg tcgtgagttc
     3001 aaagttgaag cgaacgtagg tgctcctcaa gtatcttacc gtgaaacatt ccgcgcttct
     3061 actcaagcac gtggattctt caaacgtcag tctggtggta aaggtcaatt cggtgatgtg
     3121 tggattgaat ttactccaaa cgaagaaggt aaaggattcg aattcgaaaa cgcaatcgtc
     3181 ggtggtgtgg ttcctcgtga atttatccca gcggttgaaa aaggtttggt agaatctatg
     3241 gctaacggtg ttcttgcagg ttacccaatg gttgacgtta aagctaagct ttatgatggt
     3301 tcatatcacg atgtcgactc atctgaaact gccttcaaga ttgcggcttc actttccctt
     3361 aaagaagctg ctaaatcagc acaaccagct atccttgaac caatgatgct tgtaacaatc
     3421 actgttccag aagaaaacct tggtgatgtt atgggtcacg taactgctcg tcgtggacgt
     3481 gtagatggta tggaagcaca cggtaacagc caaatcgttc gtgcttacgt tccacttgct
     3541 gaaatgttcg gttacgcaac agttcttcgt tctgcatctc aaggacgtgg tacattcatg
     3601 atggtatttg accactacga agatgtacct aagtcagtac aagaagaaat tattaagaaa
     3661 aataaaggtg aagactaatc cgtcctcact ctagaaggaa gtcacttagt ggcttccttt
     3721 tgtctttaga aaatacctct aaatatggta aaatagtaga agaataatgt gaggaaaatg
     3781 aatgtcaaat agttttgaaa ttttgatgaa tcaattgggg atgcctgctg aaatgagaca
     3841 ggctcctgct ttagcacagg ccaatattga gcgagttgtg gttcataaaa ttagtaaggt
     3901 atgggagttt catttcgtat tttctaatat tttaccgatt gaaatctttt tagaattaaa
     3961 gaaaggtttg agcgaagaat tttctaagac aggcaataaa gctgtttttg aaattaaggc
     4021 tcggtctcaa gaattttcaa atcagctctt gcagtcctac tatagggagg ctttctctga
     4081 aggtccatgt gctagtcaag gttttaagtc cctttatcaa aatttgcaag ttcgtgctga
     4141 gggtaatcag ctatttattg aaggatctga agcgattgat aaggaacatt ttaagaagaa
     4201 tcatcttcct aatttagcca aacaacttga aaagtttggt tttccaactt ttaactgtca
     4261 agttgagaag aatgatgtcc tgacccaaga gcaggaagag gcctttcatg ctgaaaatga
     4321 gcagattgtt caagctgcca atgaggaagc gctccgtgct atggaacaac tggagcagat
     4381 ggcacctcct ccagcggaag agaaaccagt ctttgatttt caagcgaaaa aagctgcagc
     4441 taaacccaag ctggataagg cggagattac tcctatgatc gaagtgacga cagaggaaaa
     4501 tcgtctggta tttgaagggg ttgtttttga tgtggagcaa aaagtgacta gaacaggtcg
     4561 tgttttaatc aactttaaaa tgacggacta tacttcaagt ttttctatgc aaaagtgggt
     4621 taaaaacgag gaagaggccc agaagtttga cctcatcaag aagaattctt ggctccgagt
     4681 tcgagggaat gtggagatga ataacttcac acgcgatttg actatgaacg tacaggatct
     4741 gcaggaagtt gttcactatg agcggaagga tttgatgcca gaaggtgagc gtcgggttga
     4801 gtttcatgct catactaaca tgtcgactat ggatgctttg ccagaggtcg aagagattgt
     4861 tgcaacagct gctaagtggg gacacaaggc ggttgctatc acagaccatg ggaatgtcca
     4921 gtcctttcca catggctata aggcggctaa gaaagcggga atccagctga tctatgggat
     4981 agaagccaat atcgtggagg accgtgtccc tatcgtctat aacgaagtgg agatggactt
     5041 atcggaagca acctacgtgg tctttgacgt ggaaacgacg ggactttcag ctatctataa
     5101 tgacttgatt caggttgcgg cctctaagat gtacaagggg aatgttattg ctgaatttga
     5161 tgaatttatc aatcctgggc atcccttgtc agcttttact acagagttaa ctggaattac
     5221 agatgatcat gtcaaaaatg ccaaaccact agaacaagtt ttgcaagaat tccaagaatt
     5281 ttgcaaggat acggtcctag ttgcccacaa tgctgccttt gacgttggct ttatgaatgc
     5341 taattatgag cgtcatggtc ttccaaagat tagtcagcca gttattgata cgctggagtt
     5401 tgctagaaac ctctatcctg agtataaacg ccatggtttg gggcctttga ccaagcgttt
     5461 tggtgtggcc ttggaacatc accacatggc caactacgat gcggaagcga ctggtcgtct
     5521 gcttttcatc tttatcaaag aggtagcaga aaaacatggt gtgaccgatt tagctagact
     5581 caacattgat ctaatcagtc cagattctta caaaaaagct cggatcaagc atgcgaccat
     5641 ctatgtcaag aatcaggtag gtctaaaaaa tatctttaag ctggtttcct tgtctaatac
     5701 caagtatttt gaaggagtgc cacggattcc gagaacggtt ctagatgccc atcgagaggg
     5761 cttgatttta ggttcagcct gttcagaggg tgaagttttt gacgtggtcg tttctcaagg
     5821 tgtggatgcg gcggttgagg tggccaagta ttatgacttt atcgaggtca tgccaccggc
     5881 tatctatgcg cccttgattg ctaaagagca ggtcaaggat atggaggaac tccagaccat
     5941 tatcaagagt ttgatagagg ttggagaccg ccttggcaag cctgttttgg ctacgggaaa
     6001 tgttcactat atcgaaccgg aagaagagat ttatcgtgaa attatcgtcc gtagtttggg
     6061 acagggtgcg atgattaacc gaactatcgg tcatggtgaa catgcccaac cagcaccact
     6121 tccaaaggct cattttcgaa cgactaatga gatgttggat gaatttgcct ttttgggaga
     6181 ggaactggct cgtaaactgg ttattgaaaa caccaatgcc ttggcagaaa tatttgaacc
     6241 cgttgaagtc gttaagggtg acttgtatac gcctttcatc gacaaggctg aagaaacagt
     6301 tgctgagttg acctataaga aagcttttga gatttatgga aatccgctgc cagatattgt
     6361 tgatttgcgg attgaaaaag aattaacatc catactgggg aatggatttg ctgtgattta
     6421 tctggcatcg cagatgctgg tgcaacgttc taatgaacgg ggttatttgg ttggttctcg
     6481 tgggtctgtc ggatctagtt tcgttgcgac catgattgga attacggagg tcaatcctct
     6541 ctctcctcac tatgtctgtg gtcagtgtca gtacagtgag tttatcacag atggttcgta
     6601 cggttcagga tttgatatgc cccataagga ctgtccaaac tgtggtcaca aactcagtaa
     6661 aaacggacag gatattccgt ttgagacctt ccttggtttt gatggggata aggttcctga
     6721 tattgacttg aacttctcgg gagaagatca gcctagcgcc cacttggatg tgcgtgatat
     6781 ctttggtgaa gaatatgcct tccgtgcggg aacagttggt acggtagctg ccaagactgc
     6841 ctatggattt gtcaagggtt acgagcgaga ttatggcaag ttttatcgtg atgcagaagt
     6901 agaacgcctc gctcaaggag cggcgggtgt caagcggaca acaggccaac acccgggggg
     6961 aatcgttgtt attccgaact acatggatgt ctacgatttt acgcctgtcc agtatccagc
     7021 agatgatgtc acggctgaat ggcagaccac tcactttaac ttccacgata tcgatgagaa
     7081 cgtcctcaaa ctcgatgtac tgggacatga tgatccgacc atgattcgaa aacttcagga
     7141 tttgtctggt attgacccta ataaaattcc tatggatgac gaaggcgtga tggcactctt
     7201 ttctgggact gatgtgctag gggtaacacc tgaacaaatt ggaacgccta cgggtatgtt
     7261 ggggattcca gagtttggaa caaatttcgt acgtggaatg gtagacgaaa cccatccgac
     7321 aacctttgcg gaattgcttc agctgtctgg tctgtcccac ggtactgatg tttggttggg
     7381 gaatgctcag gatctgatta agcaaggaat agcggaccta tcgactgtta tcggttgtcg
     7441 ggacgacatc atggtttacc tcatgcatgc gggtctggaa cctaagatgg cctttaccat
     7501 tatggaacgg gtacgtaagg gtttgtggct aaagatttca gaagaggaga gaaatggcta
     7561 tatcgaagca atgaaggcta ataaggtgcc agagtggtat atcgaatcct gtgggaaaat
     7621 taagtacatg ttccctaagg cccatgcggc agcctacgtt atgatggcct tgcgtgtagc
     7681 ttacttcaag gttcaccatc ctatttatta ctactgtgct tacttctcca ttcgtgctaa
     7741 ggcttttgat atcaagacca tgggtgcggg cttggaggtc atcaagcgca gaatggaaga
     7801 aatctctgaa aaacggaaga acaatgaagc ctctaatgtg gaaatcgatc tctatacaac
     7861 tcttgagatt gtcaatgaga tgtgggaacg aggtttcaag tttggtaaat tagatctcta
     7921 ccgtagtcag gcgacagagt tcctcatcga cggggatacc cttatcccac catttgtagc
     7981 aatggatggt ctgggagaga acgttgccaa gcaactggtg cgggcgcgtg aagagggaga
     8041 attcctctct aaaacagaac tacgcaagcg tggtggactc tcatcaacct tggttgaaaa
     8101 gatggatgag atgggtattc ttggaaatat gccagaggat aaccagttga gtttgtttga
     8161 tgagttgttt taaaaaattg cttaataatc tattaaaaga ggctaacgta tatccaatag
     8221 atttacatta gctttctttt ttgttaaaat agtctatgga aagagggtga gagtatgtca
     8281 aagatgagta taagcatccg tctggatagt gaggttaagg agcaggccca acaggtgttt
     8341 agtaatctgg gaatggatat gacaacagct attaatattt tccttcgtca ggcaattcaa
     8401 tatcagggat taccttttga tgttagacta gacgaaaatc ggaagttgct ccaagcgtta
     8461 acggatttag accaaaatcg taatatgagc cagtcttttg aatcagtctc agatttgatg
     8521 gaggacttac gtgcttaaga ttcgttatca taaacagttt aaaaaagatt ttaagttggc
     8581 tatgaagcgt ggtttgaagg cagaattatt agaagaagtt ttgaattttc tggttcaaga
     8641 aaaagaacat cctgccagat atcgtgatca ttcattgacg gcatccaagc attttcaagg
     8701 agttcgtgaa tgccataccc agccagattg gcttttggtt tataaagtag acaagtcgga
     8761 attgatttta aatttgctga ggacaggcag tcacagtgat ttattttaat ctattttaag
     8821 ggggttctca tgaaactaag aatatttgcg gaagataagc cggctaagaa ggtatttgaa
     8881 tatcaattag aacttgctga tcgtacaatt cttctatcga cagcactctt gtcaggtgct
     8941 attgctttag caggaatctt ttctgctttg aaagaaaaat aaaaatagaa aagagaaaac
     9001 agaatggttt taccaaattt taaagaaaat ctagaaaaat atgcgaaatt gttggttgcg
     9061 aacggaatta acgtgcaacc tggtcacact ttggctctct ctattgatgt ggagcaacgt
     9121 gaattggcac atctaatcgt gaaagaagct tatgccttgg gtgcgcatga ggtcatcgtt
     9181 cagtggacag atgatgtgat taaccgtgag aaattcctcc atgccccgat ggagcgtttg
     9241 gacaatgtgc cagaatacaa gattgctgag atgaactatc ttttggagaa caaggctagc
     9301 cgtcttgggg ttcgttcatc tgatccaggt gccttgaacg gagtggacgc tgacaagctt
     9361 tcagcttctg ctaaagctat gggacttgcc atgaagccta tgcgtatcgc aactcaatct
     9421 aacaaggtta gctggactgt agcagccgct gctggacttg agtgggctaa gaaagtcttc
     9481 ccaaatgctg cgagcgacga agaagcagtt gatttccttt gggatcaaat cttcaaaact
     9541 tgtcgtgtct acgaagcaga tcctgttaag gcttgggagg aacatgcagc cattctcaag
     9601 agcaaggccg atatgcttaa taaggagcaa ttttcagccc ttcactacac agcgccagga
     9661 acagatttaa cacttggttt gccaaagaac cacgtttggg aatcagctgg tgctgtcaat
     9721 gcacagggcg aagaattctt gccaaatatg ccgacagaag aggtcttcac agcgcctgac
     9781 ttccgtcgtg cagatggtta tgtcacttct acaaaaccgc ttagctacaa cggaaatatc
     9841 attgaaggca ttaaggtgac ctttaaggat ggacaaatcg tagatatcac tgctgagaag
     9901 ggtgatcagg ttatgaaaga ccttgtcttt gaaaatgcgg gtgcgcgtgc cttgggtgaa
     9961 tgtgccttgg taccagatcc aagtccaatt tctcagtcag gcattacctt ttttaacacc
    10021 cttttcgatg aaaatgcgtc aaaccacttg gctatcggtg cagcctatgc gactagcgtt
    10081 gttgatggag cggagatgag cgaagaggag cttgaagctg cagggcttaa ccgttcagat
    10141 gttcacgtag actttatgat tggttctaac caaatggata tcgatggtat tcgtgaggat
    10201 ggaacgcggg tacctctttt ccgtaatggg aattgggcaa attaaggaga taatatgtta
    10261 ggaagtatgt tcgttggtct cctagtggga tttttagcag gtgctatgac caatcgtgga
    10321 gagcgaatgg gatgttttgg aaaaatgttt ctcggttgga tcggagcctt tctaggtcac
    10381 ttgctctttg gaacttgggg gccagtttta tcaggaacag ctattatccc agcgatttta
    10441 ggagccatga ttgttttagc tattttttgg agacgaggaa gttaatttcc taaatctgat
    10501 actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca aagcactgct
    10561 ttgaggttgc agatagaact gacgaagtca gctaaaaaca ctgttttgag gttgcagata
    10621 gaactgacga agtcagtaac atatatacgg caaggcgacg ttgacgcggt ttgaagagat
    10681 ttttgaagag tatgaaacga aaaggaggtt ggtcattgta ctagcctcct tttgatatga
    10741 tataatagtt ctatgagatt agataaattt ttagttgcct gtgcggtggg aagtcggact
    10801 gaggtcaaaa acttgctcaa ggctgggcgc gtgactgtaa atggtaaaaa agaaaaatca
    10861 gctaaattgc agattgatga aaaaatagat gagattcgct ttgatgggca agtgttggag
    10921 tatgaagagt ttgtctacta catgatgaac aagcccaaag gagttatctc agcgactgag
    10981 gatcccaagc acagaaccgt tctggacttg ttggatgact tggcgcggag taaggaagtt
    11041 ttcccagtag gacgcttgga tattgacacg catggtcttt tgctcttgac caatgatggt
    11101 cagctggctc atgttcttct ttcgcccaag cgtcatgtgg acaagactta tctggcacaa
    11161 gtcaagggaa tcatgaccca agaagatgtg gagacatttg ctgagggtat tcctctcaaa
    11221 gactttacct gtcaacccgc tatactggag cttgtatcca tagatacaga aaagaatcaa
    11281 agccaaatcc gtgtgaccat tgcagaaggg aagtttcatc agatcaagcg tatggtgggc
    11341 tactgtggca aggaagtggt ggacttgcaa cgattgacta tgggaacttt agtattagat
    11401 gagaacctgg aacgagggga atggcgtcgc ttgaccaagg aagaattaga aattctccgc
    11461 gctaatatta tttaatttag tttaatactc aatgaaaatc aaagagcaaa ctaggaaact
    11521 agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa gagattttcg
    11581 aagagtataa attcgaaaaa ggtgagggac aatgtcctcg ccttttatgt tttttagttg
    11641 cttcctttgt gaaaagagtt ataatagact gtagaataaa aggaggaatc tatgaacgcg
    11701 attcaagaat catttactga taaactattt gccaactatg aagcaaatgt aaaataccaa
    11761 gcgattgaaa atgctgccag ccacaacgga atttttgcag ctctggaacg tcgccaaagc
    11821 catgtagaca acacacctgt tttctcattg gatttaacca aggacaaggt cactaaccag
    11881 aaagcgtctg gtcgttgctg gatgtttgcg gctctcaaca ccttccgcca caaactcatc
    11941 tcgcaataca aattggagaa ctttgagttg tcacaagccc acactttctt ctgggataag
    12001 tatgagaaat caaactggtt cttggagcaa gtcattgcga cttcagacca agaattgact
    12061 agccgcaagg ttagcttctt actccaaaca cctcaacaag atggcggtca atgggatatg
    12121 gtcgtttccc tctttgaaaa atacggtgtc gtgcctaagt cagtttatcc tgagtctgtt
    12181 tcatctagca gcagtcgtga gctaaatgcg atccttaata aattgcttcg tcaagatgct
    12241 caaatcttgc gtgacttgct tgtttctggt gcagatcaag cgactgttca agctaagaaa
    12301 gaagacctct tgcaagaaat ctttaacttt cttgctatgt cattaggact tccaccacgc
    12361 aagtttgact ttgcttatcg cgataaagat aacaactaca aaagtgaaaa aggaatcaca
    12421 ccacaagagt tttacaagaa atatgtcaat cttcctttag aagactacgt ttctgttatc
    12481 aatgctccaa ctgctgataa accttacgga aaatcttaca cagttgagat gttggggaat
    12541 gtggttggta gccgtgcagt tcgttacatc aacgttccaa tggagcgctt gaaagaattg
    12601 gcgattgccc aaatgcaagc aggtgagact gtttggtttg gttctgatgt cggccagctc
    12661 agtaaccgta aggctggcat ccttgcgaca gatgtttatg actttgaatc aagcatggac
    12721 attaaactta ctcaagacaa ggctggacgt ttggactata gcgaaagctt gatgacccac
    12781 gccatggtct tgacaggtgt tgacttggac gaaaatggta aatcaaccaa gtggaaggtt
    12841 gaaaactcat ggggagacaa ggtcggtaca gatggttact ttgttgcctc agacgcttgg
    12901 atggacgaat acacatacca aatcgttgtt cgtaaggaat tgctgacagc agaagaacaa
    12961 gctgcctatg gagcagaacc aattgtactt gcaccatggg atccaatggg agccttggct
    13021 gaataaaagc atagaaaaaa ggaatcagat tttagaacct ggttcctttt tagttgcttg
    13081 attacatgat gtgaagaaca tgtgccacaa tacccactgc gaagagggca aggataatag
    13141 tgattggaga tacttttttc ttaagtaagt acatgcaaag taaagtaagg agtagtcctg
    13201 ataatccagg aatcaacata tccaagtttt gttggaaagt agtaactttt tcaggagttt
    13261 gagacaatcc ttgtcctact tgtgcgaatg cttcttggat acctttagac ccttctggca
    13321 atttatccca atggatataa gccttttcat ctagttgaac tttagaaaca tcgaaagcaa
    13381 atttaatatt tacccagcgt tgaacaagga cagcaagaat gaacatccca aggatagaag
    13441 ctcctttagt gatatcttga aggataccac cagacatatc tttagtgatt tctgatccag
    13501 ccttgtatcc aatctcttga acataccaca agaatgacat acgaatcaag ttccatgcaa
    13561 caaagaagag gagtggaccc aagatattgc cagtaagggc aagtgaagca ccgagagatc
    13621 caaggattgg gcgtactgta aaccagaata ctgggtcacc gataccagca agaggtccca
    13681 tcataccgat tttgacccct tggatagcag cgtcatcgat ttccacaccg ttagcacgtt
    13741 cttcttcaag cgcaagagta acccccatga ctggagcagc tacgtatgga tgagtgttga
    13801 agaactcaag gtgacgctca agagcagcga tttgatcttc ttttttagta tagagttttt
    13861 tgatagctgg aatgagtgta taagcccagc ccaagttttg catccgttca aagttccaag
    13921 acccttgtaa gaaggttgaa cgccaccaaa cttttttacg atctgattta gttaattgaa
    13981 gtttttcagt catgatgttt tcagtccttt cttatcttag tagtcttcta ggatatcgcc
    14041 gattgggtcg ttagaagttg cggctcctcc gccaccattt ccaccagttt tagaaaggtg
    14101 aaggtagata agagcgatag caacaccgat agcaccgaat ccgattagag taatatctga
    14161 cacagcagcg agaacgaaac caagagcgaa gaatggccat acttcacgag ttgccatcat
    14221 gttgataacc atggcgtaac caacggcaac gaccatacca ccaccgatag ccataccatc
    14281 tttgagccag tctggcatgg cactaaggat actttgtaca gtttcagttg gtaccataag
    14341 gagaagagct gcaggaagcg cgatacgaag tccttggaag agtagcgcaa tgaaatgcgc
    14401 acgctccaca gcgccgaagt cacctttttt agcggcagca tctgcagtat gaaccaaacc
    14461 aactgaaatt gtacgaacaa tcattgtcaa gaaaagtcca gctacggcaa gagggatagc
    14521 aaccgcttgg gcaacaccga taccagtctt ggtaaagtca ccaccaagaa ccatgataat
    14581 ggcagcagcg acagaagcaa gtgcagcatc aggagcgata gcagcaccga tatttgacca
    14641 accaagggca atcatttgaa gcgatccacc gaggataatc cctgcttcca agtgacctgt
    14701 tacaagccca ataagggtac aggctacaag tggttggtga aattggaact ggtcgaggat
    14761 gccttcaaga cctgcaaaga aggctacaac gactactaaa accatagaaa taatagacat
    14821 gtttaaaatc ctttcataaa taatggctta tttgacattg gctttgttaa tcaagtcaaa
    14881 caaatctttt ttagaatcat ttggtacttt acggacatca aattcaacac ccaagtcacg
    14941 cattttttca aatgtcgcaa catcttcttt gtccatagac aaaacggtat tgaccaatgt
    15001 tttacctgtt gagtgagcca tagaaccaac gttaagagtc ttgattggca cgccgccttc
    15061 gatggcacga agggcatctt gaggtgtttc aaacaagata agggcatgtg tttctccaaa
    15121 acgtgggtct tttgaaatct caatcagttt ttgaattgga accacgttag ccttgacatt
    15181 acctggagct gcttgtttaa tcaattcttt acgaaggtcg tctttagcca cgttatctga
    15241 agcaacgatg atacgatttg cttttgaatc tggagtccaa gcagttgcaa cctgaccgtg
    15301 aagtagacgt gtgtcaagac gggcaagatt gattttcaat ttaccgtctc cgataacagt
    15361 tccttctggg atagcagctt gggcaactgg agcagctgca gcgcttgcaa cttcttcaac
    15421 tggatttagc tcttctggaa gagctttgat gccatccttg gcttctttaa tgatattagc
    15481 agcgactttt tctacacctg cagcagcgtc catgaggcgc tctgtgtagg cttgaatcaa
    15541 catcggtaag ttaagtcctg tgatgatggc aaacttacgc tcaggatttt ctcccatcac
    15601 gcgactagct tggttaaatg gagaaccact ccaaaggtca gccaaaacta gaacctcatc
    15661 ttctgcgtca aatgcagcaa cagcgttatt aaacttagcg tatagatcat caggaccttc
    15721 atttggcata aaggtcacaa cttgaacctt ttcttgttca ccaaagatca tagatcctga
    15781 ctgatgaata cccgcagcaa attcgccgtg gctcgcaata atgattccga tactcattat
    15841 tgtcattcct ccttttttgt tttagttttc atttaagaaa actttaagac tgtttaagta
    15901 taaaccgttt tcataaaaaa agcaactatt tatcataaaa aattaaaaaa tttttatttt
    15961 tatatgttga tatgttgagt ttttaaggag taatcttgaa ttcttattta aaaatttaat
    16021 ataaaaaagt tagaagaagc ttccaacttt taaaataggt ttctattaga ttagtgggtg
    16081 aagtcgagta ccatacgtcc ttggatttga cctttttcca tttcgtcgaa aatggcaaca
    16141 gcatcttcta ctggacgttt ttgaacaact ggggctacca gaccttctgc accaaattgg
    16201 aaggcttctt ctaagtcttt acgagttccg acaagagaac cgatgacttg gattccatcg
    16261 aggactgttt taacgatact gagttccatc atttcagaag gaagaccaac agcgacgacg
    16321 cgaccaccag cacgaacgga gtcaacagcc tggttgaagg caactttaga cacagcagtt
    16381 acgacagctg aatgagctcc tccatcagtt tttcctttaa tgagtccagc tacatcttca
    16441 acttcgaggc cgttaatcac aatgtcagcg cctacttctt ttgcaagggc aagtttgtca
    16501 ttgttgatat cgactgcgat aacatgagca ttgaatactt ttttagcgta ttgaacagcg
    16561 aggttaccaa gtccaccagc accgtaaaga acaacccatt ggcctggttc aacttttgct
    16621 tctttgatag ctttataggt tgttactcca gcacatgtga tagaagaagc ttgggctgga
    16681 tcaagtccgt caggaacctt gacagcatag tcagcagtta cgatacattg ttcagccata
    16741 ccaccgtcta ctgagtagcc agcatttttc actgtacggc aaagggtttc gcgaccagtt
    16801 gtacagtatt cgcaagtgcc acatccttca aagaaccaag caacgctgac gcggtcaccg
    16861 actttaaggc ttttcacatc tggagcaatc tctttaacga taccgatacc ttcgtgccca
    16921 agaacacgtc ctgggacttg accaaagtca ccatgagcaa cgtggagatc ggtgtggcaa
    16981 acgccacagt attcaacttc tacaagtgct tccccagttt caagtggacg gagtactttt
    17041 tcttcaatag caacaccagt gctttctgga tttacaacaa cagctttcat agctatcctc
    17101 cttgatattg accgagaaca gggacaacgg gactggattg attttttgtc aacagagtcg
    17161 agtgtgacga gctctcttgt atttatatgt gaagtatatc acaaaagaaa gccttttcca
    17221 aggttttgga gacaaaaagt agaacaatcg attaactggt tgatgagact gtgaaaagag
    17281 cacaaagacc agcttgcaag ctggtcatac tcttcgaaaa tctcttcaaa ccacgtcagc
    17341 ttcatctaca acctcaaagc agtgctttga gcaacctacg gctagcttcc tagtttgctc
    17401 tttgattctc attgagtatc agtataggtt ataagaataa gtcttgtagg gttttggcaa
    17461 ccccatcttg gtcgttggta agggaaattt gctcatctgc ataagggagt agctctggat
    17521 tggcattttt catggcataa cccttcccag caaaagcgag catttcggta tcattgtgtt
    17581 catctccaaa ggcaatcaaa tcttttttgt cacgattcat tatcttgagc aagtagtcca
    17641 aagcaaaggc cttgttgaca ccttttgggg tacattcaag gatattgagc ggacctcccc
    17701 aggtattgat agacagttga tgctggtaga aggcgttcat ttcttttgcc agagcatatt
    17761 tgtcactggc tctagtctgt aacagaatac agttaggatc cttggttact aactcaggct
    17821 gaaattgatt ttcaggctgg aaagcttcta caccaaatag tttgggatta gcaatttctt
    17881 cattgggatt tgtaatgtaa aattttttac ggtattctcc agcgataaaa tcggcttgaa
    17941 tgtcctctga acgtcgaacc atgtctagca gatatttttt gtctacagtt aaacactttt
    18001 caaaatccca aacttggtct ggtaaatgag taagggagcc gttgaaatta atcataggag
    18061 tgtttaagcc tagttcatgg taaaaatctt ttgacatgcg gtaagggcga cccgtcgtaa
    18121 taataatctg atggcctttt tcagcaactt ttttaatggt ttctttggta aagtcagaaa
    18181 tttgactgtc tgagttgagc agggttccat ccaggtcaac tgcaataatt tttttcgtca
    18241 tagggacctt tctagtgtct tttcagataa gatgtctact attataacag aaagcaggca
    18301 gaaaagcaga ttcgaaacaa aaaaagaaat ttcatcaaat tcttaaataa atcaaacaaa
    18361 gatattgaaa aagtgaatga taaatgatat aattctatta ttgttcgtaa aaattaaaag
    18421 gagattgata atggacaaat tatttaaact aaaagagaac ggtacagacg ttcgtacaga
    18481 ggttctcgct ggtttaacaa ctttctttgc aatgagctat attctctttg taaacccaca
    18541 aattctttcg caaacaggaa tgcctgctca gggtgtattc ctcgcaacga ttattggtgc
    18601 agttgctggt accctgatga tggccttcta cgctaactta ccttatgccc aagcgccagg
    18661 tatgggactc aatgccttct ttacctttac agttgtattc ggacttggtt attcttggca
    18721 agaagcccta gctatggtct tcatctgtgg aattatctca ttgattatta ccttgacaaa
    18781 tgttcgtaaa atgatcattg aatcgattcc caatgctctt cgctcagcta tttcagctgg
    18841 tatcggtgtc ttccttgcct atgtagggat taagaatgct ggacttttga aatttacgat
    18901 tgatccaggt aactatactg ttgtaggaga aggggctgac aaagctcaag caacgattgc
    18961 agcaaactct tcagcagttc caggattggt cagctttaat aatccagctg ttttggtggc
    19021 tcttgcagga ctcgctatca caattttctt tgtcatcaaa ggaattaaag ggggaattct
    19081 cctctctatc ttgacaacaa ctgttcttgc tattgcagtt ggtttggtag atttgtcaag
    19141 catcgatttt gctaataacc atgttggtgc agcttttaaa gatttgaaga caatctttgg
    19201 tgcagctctt ggttcagaag gtttgggagc tttgatttca gatacagctc gcttgcctga
    19261 aactctgatg gctattcttg ccttttcatt gacagatatt tttgatacaa ttggtacctt
    19321 gatcggtaca ggtgaaaaag ttggtatcgt agcgacaaat ggtgaaaatc atcaatcagc
    19381 taagttagac aaggctcttt actctgactt gattggtact tcaattggtg ccatcgcagg
    19441 tacttcgaac gtaacgactt atgttgagtc tgctgctggt atcggtgcag gtggacgtac
    19501 tggtttgaca gccttggtcg tggcaatctg ttttgcgatc tcaagcttct ttagcccact
    19561 tctagcgatt gtaccaacag ccgctacagc tccaatcttg attatcgttg ggattatgat
    19621 gttggctagc ttgaaaaata tccattggga cgatatgtct gaagcggttc ctgctttctt
    19681 cacatctatc tttatgggat tcagctactc tatcactcaa gggattgcag ttggtttctt
    19741 gacttacact ttgactaagc ttgtcaaagg tcaagctaaa gatgttcatg tcatgatttg
    19801 gattttggat gctttgttta tccttaacta catcagtatg gcactataaa tgctataata
    19861 taaaaagggg ttctcccctt tttatattat agttacttga aaattgcttc aaactaggta
    19921 ggtgattttg attgaatggt aggagtataa ttgatgataa aaaagaatat ttgggatgaa
    19981 gtattaaaca gggggaagtg gttaataatc tttctagtag ggcttttgtt ttctcagttt
    20041 cctttagccc tagcaacttt tttaactagc agaaaatttc cactgcttca aacgggacta
    20101 ttagtagggg ccttatctct tgtggtttta actgtattta taataggtgc taggaaaaca
    20161 cagttggcta gttttggttt atcgtttttc aaggctaagg atttggctag attggcctta
    20221 agctatctag tgattcttgc ctttaatatt cttggtgtgg tcttactgca tttgatgaat
    20281 gaaacaacaa ccagcaatca atcaaatatt aatgatttgg tccagaatag ttccttaatt
    20341 tccagttttt tcttactgct tctaatagct cctatttgtg aggagatttt atgtcgagga
    20401 gtgattccta aaaaactctt tcgtggtaaa gaaaatgtgg gttatatagt cggtgctatt
    20461 atctttgcct tgcttcattt accgacaaat ctaccttctc ttcttatcta tggaggaatg
    20521 tcaactgtgc taacttggac agtttataaa acccaacgtt tggaaatgtc tatcttactt
    20581 cacatgattg ttaatggaat ttttttctgt ttgttggctc tcgtggtgat tttgagtcgg
    20641 acattaggga tctctgttta agatttttga caattgctta cttgtttctg ctgggaaaaa
    20701 gatgaatgca atcgtgtcca tctttttctt tttatggtaa aatagagaaa taatatgatg
    20761 aaaagccttg agggagtgac cgatatgtca actaaagcca atcatgcaaa gacagctatt
    20821 tgcggaatta tcaatgtaac cccagactcc ttttcggatg gtggtcaatt ttttgctctt
    20881 gagcaggcac tccagcaggc tcgtaaattg atagcagaag gggctagcat gctagatatc
    20941 ggcggagaat cgactcggcc gggaagtagc tatagctatg ttgagataga agaggaaatc
    21001 cagcgtgttg ttccagttat taaggctatt cgcaaggaaa gtgatgtcct catctctatt
    21061 gatacttgga agagccaggt agcagaggct gctttggctg ctggtgccaa tctagtcaac
    21121 gatatcactg gtctcatggg cgatgaaaaa atggctcgtg tggttgctaa ggctggagcg
    21181 aaagtggtta tcatgtttaa tccagtcatg gctcgtcctc agcatcctag ctcgctcatc
    21241 tttcctcatt ttggctttgg tcaagctttt acagaggaag agttagctga ctttgaaaca
    21301 ttgccaatcg aagacttgat ggatgctttc tttgaacgag cactagcgag agcaaatcaa
    21361 gctggaattg cacaagaaaa tatcctgttg gatctgggaa tcggctttgg tctgaccaag
    21421 aaagaaaatc tgcttctttt acgggacctg gataaactac atcagaaggg ctatccaatc
    21481 tttcttggag tgtcgcgcaa gcggtttgtc atcaatatcc ttgaagaaag tggttttgaa
    21541 gtcaatcctg agacagaact tggtttccga aatcgggaca cggcttcggc tcatgtaact
    21601 agtatcgctg cgagacaggg tgtagaagtg gtgcgcgtgc atgacgtagc tagtcacagg
    21661 atggcagttg aaattgcctc tgccattcgt ctggctgatg aagcggaaaa tttagattta
    21721 aaacaatata aataagatga aagaaattga aaataatcag tggattgcta actaccggac
    21781 ggatcaaccg cattttggct tggaacgaat ggtggaactg ttagccttgc gtggaaatcc
    21841 ccatctcaaa ctcaaggtca tccatatcgg agggactaac ggcaagggtt cgaccattgc
    21901 ttttttgaag aatatgctgg aaaagctagg gctgagagtt ggggtgttca gctcgcccta
    21961 tctcattcat tacacagacc agattagcat caatggggaa tccattccag aggctaggct
    22021 agaagccctc atggcagact atcagtcttt gctggaggga gaagcggtcg ccaatttaca
    22081 gagtacaacc gagtttgaga ttatcacagc cctggcctat gactactttg ccgcagagca
    22141 agtagaagtg gctatcatgg aagtcggtat gggtggactt ttagatagta ccaatgtctg
    22201 tcagcccatt ttgacaggaa ttacgactat tggcttggac catgtagccc tacttggtga
    22261 taccttggaa gccatagcag agcagaaagc tggtattatc aaacaaggta ttcccttggt
    22321 gacaggtcgt atcgctccag aagctttggc tgtgattgac cgcattgcgg aagggaaaga
    22381 tgcgccgaga cttgcctacg ggacagatta tcaggtcagc catcaaaaga gtgtggttac
    22441 aggcgaagtc tttgactata caagtgttgt caggcaaggt cgcttccaaa ctggcctgct
    22501 tggcttgcac cagatagaga atgctgggat ggccatagct ttacttgata ctttttgtca
    22561 agaacatggt cgagagctag caagcaatga cctgcttggt caagccttgg aagaaacaag
    22621 ttggccaggg cgtttggaag ttgtgtcgag agaccccctg atgattttgg atggagccca
    22681 caatccccat gcgatcaagg ccttattggc aaccttgcaa gaacgctttg cggattatcg
    22741 taaggaaatc ctctttactt gcatcaaaac caaggcctta gaggatatgt tggacttgct
    22801 gggggccatg ccagataccg agctaactct aacacatttt gacgatagtc gggcgactga
    22861 tgaaagcgtg ctggaagagg cggctaagtc tagaaatctc aactaccaag gttggcagga
    22921 ttttctagag cagaaattga cagataaaaa agaagagaaa aaaacagtta ggattgtcac
    22981 gggttccttg tatttcttga gccaagtgag agcctatctg atggagagga aaaacgaaaa
    23041 tggatacaca aaagattgaa gcagctgtaa aaatgattat cgaggctgtc ggagaggacg
    23101 ctaaccgtga gggcttgcag gaaacgcctg ctcgagtggc ccgtatgtac caagagattt
    23161 tttcaggtct tggtcaaaca gcggaggaac atttgtcaaa atcctttgag attattgatg
    23221 acaacatggt agtagaaaag gatatctttt tccacactat gtgtgagcac cacttcttgc
    23281 cattttatgg tagagcgcac atagcctata ttccagatgg tcgtgtggca ggtttgtcta
    23341 agctagcccg tacggttgaa gtttattcga aaaaaccaca aattcaagaa cgattgaata
    23401 tcgaagtggc cgatgccttg atggagtatc taggtgctaa aggagccttt gttgtcattg
    23461 aggcggaaca tatgtgtatg agcatgcgtg gtgtcagaaa accaggcact gcaaccttga
    23521 cgacagtagc tcgtggtcta tttgaaacag ataaggacct ccgagatcag gcttatcgtt
    23581 taatgggact ataatattct tcgaaaatca aattcaaacc acgtcagcct ccatctgcaa
    23641 cctcaaaaca gtgttttgag cagcctgcgg ctagcttcct agtttgcact ttgattttca
    23701 ttgagtataa aaagaatccg ctttgggcgg atttttcgag aaaggaatca ttatggatca
    23761 actgcagatt aaggatttgg aaatttttgc ctatcatggt ctttttccga gtgagaaaga
    23821 attggggcaa aagtttgtcg tttcagctat cctattctat gatatgacca aggcggcgac
    23881 agatttggat ttaacagcct ctgtccatta tggagaactg tgtcagcagt ggacgacttg
    23941 gtttcaggaa acaactgagg acttgattga aacggtagcc tacaaactag tagaacgtac
    24001 ctttgaggct tatcctcttg tccaagaaat gaagctggaa ctgaaaaaac cttgggcacc
    24061 ggtgcatttg ccactagata cttgctcggt aaccattcat cgccgcaagc aacgagcctt
    24121 tatcgcccta ggaagcaata tgggagataa acaagcaaac ttgaaacaag ccattgagaa
    24181 aatgcgaaat caaggtatcc atattctcaa agagtccagt gtcttgacga cggagccttg
    24241 gggtggtgtg gagcaagata gctttgctaa tcaagtggtt gaggttgaaa cctggctacc
    24301 ggcaccggtc ttgttagaaa gcttgttagc cattgagtca gagatgggac gggtgagaga
    24361 agtacattgg gggcctcgtt tgattgattt ggacttgctc tttgtggagg accagatcct
    24421 ttatacagat gaccttatct tgccccatcc ttacatagcg gaacgccttt ttgtccttga
    24481 gtccttacag gaaattgcgc ctcattttat ccatccgaca ttaaagcaac cgatccgcaa
    24541 cttgtataat gctttgaaac aatagaaaaa actctagttt tcagtagttt gtaactgaag
    24601 gctagagttt ttaaaatagg tcattttctt ctgggaggag gatagtttct ctaccgtcca
    24661 tgtctaaaac cagtactctt gggggataac gagggtcgaa aggatggtta aagtcaaaat
    24721 caatggctgt agggaggtgt tgacttgaaa agtggaaggt aatctttcct tggttattaa
    24781 gcaattgaaa ctcgagttct tcttccaatt caaagacatt ttttaagaaa tggtcgataa
    24841 tataccaaaa agagtcaatg atgtcatcag gcaagctggt aacaatgcca aaactagcag
    24901 accgcatgtg ggtattggta aaagccatat ctctgtctcc tttcttttcc cttatcatac
    24961 agcaaatagg attaaaaatc aagaaaaggt gattttttga aaaggatttt aactacaggg
    25021 aaaaagaagg aaaaaaattc ccaaaaatct accgaagtta ataggtaaat tcccaaatta
    25081 acttgattat ataactttca gttactttga gaagttaccg aaaaatattt ttcatatcta
    25141 ttgactttta ggggtgaaat ttggtatgat agtaggcggt attgtttacc ccatttgaaa
    25201 ggccccggaa ccttccaaat acttttcgat gggaaggaac acccatcacc gtaaacaaaa
    25261 atcgaactat atataggaga aatcatgaac aaaacaacat ttatggctaa accaggccaa
    25321 gttgaacgta aatggtacgt agttgacgca actgatgtac cacttggacg tctttctgca
    25381 gtagttgcta gcgtacttcg cggaaaaaac aaaccaacat ttacaccaca cactgataca
    25441 ggtgacttcg tgattgttat caatgctgaa aaagttaaat tgactggtaa aaaagcaact
    25501 gataaaatct actacactca ctcaaaccac ccaggtggat tgaaacaaat ctctgcaggt
    25561 gaacttcgtt ctaaaaatgc agtacgtttg attgagaaat cagttaaagg tatgcttcca
    25621 cacaatacac ttggacgcgc tcaaggtatg aagttgaaag tatttgttgg agctgagcac
    25681 actcatgctg cacaacaacc agaagttctt gacatttcag gacttatcta aggaaaggaa
    25741 caaataaagt atgtcacaag cacaatatgc aggtactgga cgtcgtaaaa acgctgttgc
    25801 acgcgttcgc cttgttccag gaactggtaa aatcactgtt aacaaaaaag atgttgaaga
    25861 gtacatccca cacgctgacc ttcgtcttgt catcaaccaa ccattcgcag ttacttcaac
    25921 tgtaggttca tacgacgttt tcgttaacgt tgtaggtggt ggatacgctg gtcaatcagg
    25981 agctatccgt cacggtatcg ctcgtgccct tcttcaagta gacccagact tccgcgattc
    26041 attgaaacgc gcaggacttc ttacacgtga ctcacgtaaa gttgaacgta agaaaccagg
    26101 tcttaagaaa gctcgtaaag catcacaatt tagtaaacgt taattcgaaa gaattactat
    26161 acttatacag agcacctttc ggggtgttct ttttttatac tttcttacta aattggtgca
    26221 attgacacag ttgttgcgac tttagtcgct tacaaatgtg gctgcaacct gacaaggtca
    26281 gttgcctcaa aacgttaatc aatacaatta tatcaacgtt tcaaagcact caagagttta
    26341 cctcatgggt gcttttttta tgttttttga aaaagttcac ctcaaagttt accttatttt
    26401 caccttattg ttttagagac tgtaaggcaa attcaccgaa ggccataggg actacattag
    26461 aagtattgac ataaatctgt gttgtacctg tgtcgctatg tattagagct tcagaaatgg
    26521 cttctaagct cattcctgct tgtttagcga gcgttgctcc ggtatgacgt agtttgtgtg
    26581 gcgtagcgtg tgtaagctct gggtgtcgtt ttctgatagt tttcatctta ttattgagat
    26641 agtcagaatg taaaggttta ttgatattgc ctttggtatc tatgtaagta aagatgaatt
    26701 gttctggatt actgatgata ccaaactttg ccagttcata tttttgttgt tttttccatg
    26761 aagtcagaag ttgaagt
//
LOCUS       DAYYQN010000026        25855 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYYQN010000026 DAYYQN010000000
VERSION     DAYYQN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.026"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(231..1793)
                     /gene="guaA"
                     /locus_tag="ACHBRQ_001031"
     CDS             complement(231..1793)
                     /gene="guaA"
                     /locus_tag="ACHBRQ_001031"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9592721.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1935..2633
                     /locus_tag="ACHBRQ_001032"
     CDS             1935..2633
                     /locus_tag="ACHBRQ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9592722.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2676..3572)
                     /locus_tag="ACHBRQ_001033"
     CDS             complement(2676..3572)
                     /locus_tag="ACHBRQ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFM9592723.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLSYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQANIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3581..4516)
                     /locus_tag="ACHBRQ_001034"
     CDS             complement(3581..4516)
                     /locus_tag="ACHBRQ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9592724.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYSGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQTFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4513..5238)
                     /locus_tag="ACHBRQ_001035"
     CDS             complement(4513..5238)
                     /locus_tag="ACHBRQ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HFM9592725.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5322..5957)
                     /locus_tag="ACHBRQ_001036"
     CDS             complement(5322..5957)
                     /locus_tag="ACHBRQ_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9592726.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQDL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5959..6786)
                     /locus_tag="ACHBRQ_001037"
     CDS             complement(5959..6786)
                     /locus_tag="ACHBRQ_001037"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9592727.1"
                     /translation="MMIKIIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6808..7143)
                     /locus_tag="ACHBRQ_001038"
     CDS             complement(6808..7143)
                     /locus_tag="ACHBRQ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFM9592728.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7144..7860)
                     /locus_tag="ACHBRQ_001039"
     CDS             complement(7144..7860)
                     /locus_tag="ACHBRQ_001039"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFM9592729.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEQRDQAPLVIKEVNGIKVALLAYSYGFNGIEQYISQEDYNRYLLD
                     LNEDKMKAEVERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            8373..8984
                     /gene="def"
                     /locus_tag="ACHBRQ_001040"
     CDS             8373..8984
                     /gene="def"
                     /locus_tag="ACHBRQ_001040"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9592730.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9029..9757
                     /gene="rlmB"
                     /locus_tag="ACHBRQ_001041"
     CDS             9029..9757
                     /gene="rlmB"
                     /locus_tag="ACHBRQ_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9592731.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKNEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9796..10707)
                     /gene="trxB"
                     /locus_tag="ACHBRQ_001042"
     CDS             complement(9796..10707)
                     /gene="trxB"
                     /locus_tag="ACHBRQ_001042"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9592732.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLGDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10773..10997)
                     /locus_tag="ACHBRQ_001043"
     CDS             complement(10773..10997)
                     /locus_tag="ACHBRQ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HFM9592733.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11075..11818)
                     /locus_tag="ACHBRQ_001044"
     CDS             complement(11075..11818)
                     /locus_tag="ACHBRQ_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9592734.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11818..12618)
                     /locus_tag="ACHBRQ_001045"
     CDS             complement(11818..12618)
                     /locus_tag="ACHBRQ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592735.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12776..13132)
                     /locus_tag="ACHBRQ_001046"
     CDS             complement(12776..13132)
                     /locus_tag="ACHBRQ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9592736.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13126..13656)
                     /locus_tag="ACHBRQ_001047"
     CDS             complement(13126..13656)
                     /locus_tag="ACHBRQ_001047"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9592737.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13656..14150)
                     /locus_tag="ACHBRQ_001048"
     CDS             complement(13656..14150)
                     /locus_tag="ACHBRQ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9592738.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14285..14731
                     /locus_tag="ACHBRQ_001049"
     CDS             14285..14731
                     /locus_tag="ACHBRQ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9592739.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQILNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14728..15375
                     /locus_tag="ACHBRQ_001050"
     CDS             14728..15375
                     /locus_tag="ACHBRQ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9592740.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15396..15977)
                     /gene="pdxT"
                     /locus_tag="ACHBRQ_001051"
     CDS             complement(15396..15977)
                     /gene="pdxT"
                     /locus_tag="ACHBRQ_001051"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HFM9592741.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15978..16853)
                     /gene="pdxS"
                     /locus_tag="ACHBRQ_001052"
     CDS             complement(15978..16853)
                     /gene="pdxS"
                     /locus_tag="ACHBRQ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HFM9592742.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17005..18384)
                     /gene="nox"
                     /locus_tag="ACHBRQ_001053"
     CDS             complement(17005..18384)
                     /gene="nox"
                     /locus_tag="ACHBRQ_001053"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HFM9592743.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18678..19601
                     /locus_tag="ACHBRQ_001054"
     CDS             18678..19601
                     /locus_tag="ACHBRQ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9592744.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVINKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19661..20266
                     /locus_tag="ACHBRQ_001055"
     CDS             19661..20266
                     /locus_tag="ACHBRQ_001055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9592745.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFSDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20285..21529
                     /locus_tag="ACHBRQ_001056"
     CDS             20285..21529
                     /locus_tag="ACHBRQ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9592746.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21752..22009)
                     /locus_tag="ACHBRQ_001057"
     CDS             complement(21752..22009)
                     /locus_tag="ACHBRQ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9592747.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22051..24087)
                     /gene="glyS"
                     /locus_tag="ACHBRQ_001058"
     CDS             complement(22051..24087)
                     /gene="glyS"
                     /locus_tag="ACHBRQ_001058"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9592748.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24299..25216)
                     /gene="glyQ"
                     /locus_tag="ACHBRQ_001059"
     CDS             complement(24299..25216)
                     /gene="glyQ"
                     /locus_tag="ACHBRQ_001059"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9592749.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25229..>25855)
                     /locus_tag="ACHBRQ_001060"
     CDS             complement(25229..>25855)
                     /locus_tag="ACHBRQ_001060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592750.1"
                     /translation="FSLLLLVFCILIGIFLIYNGLSDWTKFEDLDYEEKALKISEINK
                     RITKASPQKIEESIKSDLKLEQLTEETSEKIKYYQSIENSVVRRIYETSPVNYKIVSD
                     SILNRFLYDVIAIGQDMLHKDIIFEIKYLQNNIGSSVLDRYITKLYELSDNYSIETNR
                     KPYKKLILVTTNEVFCSIDKLINMQKKRNNFSIIVLKENEIKKTNFFN"
BASE COUNT         7667 a         5772 c         4651 g         7765 t
ORIGIN      
        1 taaactccaa aaaacttgat tttggagtag ttataaaaac tcattcagat aatttattat
       61 ttgattttga tacgattcct gaaaatataa tagtagttga ccattttgaa cttacaatcg
      121 aaaaatggtt aagtaaaagt caacagttac tatagtttgc aaattattta cagaaagaac
      181 aaaaaatgct ctgaaacact gatttcaaag cattttttgt tagtttaaaa ttactcccac
      241 tcaactgttg caggtggttt acttgtaata tcgtagacga tacggttaac atgatccact
      301 tcatttacga tacgtactga gattttttga agtacttccc atggaatttt ggcaaaatca
      361 gcagtcatac catcgataga agtgatagca cggattgcaa tcgtgtagtc atacgtacga
      421 ccgtcaccca taacaccgac tgaacgaacg cctgtgttaa cagtgaagta ttgccaaata
      481 tcgcggtcaa gtccagcttt agcgatttct tcacgaagaa tagcgtctga ttcacgaacg
      541 gtttcaagtt tctcttcagt gatttcaccc atgacacgga tagcaagtcc tggtcctggg
      601 aatggttggc gccatacgat atggtctggc ataccaagct ctgtaccaag agcacgaact
      661 tcatccttgt aaagagtatt aagtggttca atcaattcaa actgcatatc ttctggaaga
      721 ccacccacgt tgtggtgtga cttgatagtt tgagctgtat ccgtaccaga ctcgataaca
      781 tctgtgtata aagtaccttg agcaaggaat ttcacatctt tgagcttgct tgcttcgtca
      841 tcgaatacat agacaaactc gttaccgatg attttacgtt tttgttcagg atcagaaacg
      901 ccagcaagtt tgtcaaggaa acgtttagca gcgtctgctt tgacgatatt caaaccaaac
      961 ttaccaccga gcatgtccat aacttgatca gcttcgcctt tacgaagaag accgtggtct
     1021 acgaagatac agatcaattg atcgccaatc gctttttgga gaagaacccc aacgacagat
     1081 gagtcaacac cacctgatag accaagaagg acacgtttat caccgacggt ttcacgaatt
     1141 tttttgatct gcatgtcaat gaaattatcc attgaccagt cacctttagc cttacaaatg
     1201 ttaagggcaa agttacgaag gatatcattt ccgtatacag aatgacgaac ttctgggtgg
     1261 aattggatac cgtaaatgtg tttatctggg ttttcgatgg ctgcgtatgg gcagtcagct
     1321 gatgtacctg tacgaacaaa gtcagcagga atctcagtaa ccgcatcacc atggctcatc
     1381 aaaacagtct gttcatcagg tgttgattca aaaagcgctg atggtgtgtg agttagggtt
     1441 gattgaccgt attcacgatt tccagcatca cctgcaggaa caacttttcc tccaagttta
     1501 tgggtcaata actgcatacc ataacagatt cccaaaattg gaattccgag ttcgaagatt
     1561 tctgggtcaa tatcaaatga accatcttca tatacagaat ttggaccacc tgatagaata
     1621 attcctacag gattgacttc acgaacttca gcagctgaaa ttttatggct ttttagttct
     1681 gaaaaaacac caatctcacg gatacggcgt gaaatcagct ggttgtactg gctaccatag
     1741 tccaatacga tgattttttc tacatcttgc aaatcagttg aaatgttgct catctttttc
     1801 ctttctcaaa agaaatatct tttctcaata ttctatcaca aataagggcg aattaccaac
     1861 taaacaaggc gaagtttgac tttttctagt ttattggggt acaatagaga aaagatagaa
     1921 atgaagtgaa aaatatgcta cctgcttata tgaaaatcca tgatcagata aaaaaggata
     1981 ttgacgagca ccgttgggct attggtgaga ggcttcccag tgaaagagat ttagctgagc
     2041 agtttgcggt cagtcgcatg accctccgcc aagccgtatc tctattagtc gaagaaggcg
     2101 tcttagagcg ccgtgtagga agcggcacct ttgtttccag tactcgagta caagaaaaga
     2161 tgcgagggac aaccagtttt actgaaattg tcaaatccca aggtaaagtt ccctctagcc
     2221 agctcatttc ctacaaaaaa accattccca atgagcaaga agttgccaag ctaggaattt
     2281 ttccaacgga caatattatc cgaatggagc gggtccgcta tgccgaccaa gttcccctag
     2341 tttatgaagt tgcttctatt cctgaaaaat tcattaagga ctttaaaaaa gaagaaatca
     2401 ccagtcattt cttccaaacc ttgcagaaac atggctatcg tatcggcaaa tctcaacaga
     2461 ccatctatgc tcgccttgct aaagaaaaga ttgcccacta tttggaagtt gaaaaaggac
     2521 atgctattct tggattgaca caggtttcct acctagaaga tggtactgct tttgaatacg
     2581 taaaaagtca atatgtaggc gaacgctttg aattttatct tgaaaataat tagattacca
     2641 cgcaaaaact ctctgtcaca gacaaagagt cttttttatt tttctaagaa taaatctttc
     2701 aaagaaatca aagttacagc aaacaatatc attgccatac caagaaaatc aatgggataa
     2761 aattgttcat tcattattaa gaaggcaaag aaaaccgccg atattggctc aattgaagcc
     2821 aacaagcttg acttgaccgg tcctatcaga ctggctcctt taaggaaagc tgtataggca
     2881 aagacagtcc cgataaggat aatgcctgca aacgcaagga gaaaatcaag actagtcggg
     2941 atattggcct gtagaacccc tgtaaaagga agggcgacca aacctgctat aaccattccc
     3001 acaccaatga ccaagctact cccccacttt ttaatcaagg ctatgggtaa aatgatatat
     3061 agagcataag tcaaggcaga aaagagaccc cagaacaggc cagcaggtgt catggataac
     3121 tggtccaact gcccatgtgt tgcgatcagg aaggttcctc cgatggcgaa tatgatggaa
     3181 actatctctc ccagtgtcgg tgccacccta tccttgatac agctataaat taaaattccg
     3241 acaggacaaa catactgaag caccgtcgct gttcccgcat tggtctcctg aatagcagac
     3301 agataggcga attggttgag aaaaagacca atcagagcaa aaataagaag agacagcaaa
     3361 ctctttctat cctttaaaaa gaccagtatt ttatcctttg cagtagcata agacaagagc
     3421 atgagaattc caccagcgat taaaagacgc aagttggtca agaccagagc cgaaattccg
     3481 tgtgccatta ggtattggcc actcgttcct gacaaccccc aagcaatacc agccacaact
     3541 gttagtaaag tcccttttaa actatttgac atgtctctcc ttactcttct ttgcgaatca
     3601 agttcatcac ttgattgcgg tctaatatcc attgagctct ctcgtccttt tcataggtac
     3661 gattcgatag gaaaatagtg gcttcttgct tctgacgatt ccacatgata aaggtacctg
     3721 tatagcccgt atggtctagc caatctcctt ccaaattcca tgctaaagaa cgttccttgt
     3781 catccaaagg agaaaaattt tgatttaagt ctcttgcaaa atcatctgct aaatagtgtt
     3841 ctaaaaaggt ttgtaaatcc tttatagtcg aaaataaacc agcactccca gcatgtctac
     3901 ccaggagacg agccttggga tcatgcacta tgcctgcctc tacacctcta actgttggaa
     3961 cagcaagctc aactggccca aacttagttt ccgtcattcc ccaaggtttc cagacttgat
     4021 cctttaaaat cacatccaaa tcttgattaa aaattctttc caaaataaag cccaacagca
     4081 aaaaatggac atccgaataa agaaaggctg gctgacttcg tctgttgaga tgaaacatcg
     4141 cttcctttaa ttcaggggct gttaaaagat cacgattagg aataaaagga tcaaggtctg
     4201 ttgcatgagt caagagctgg cgaatagtga tgtctggata atcactctca ggtaaaaaat
     4261 ctattaccag tctatcaata tctaattgac ctatttccca caagaaggta caaactgtgc
     4321 caaccccaac aaccttactg acactagcta ggtcataaac tagtcctgcc tcagtctgca
     4381 agccatgctc tgggtcactc tggcctgaat agaactctgt ccattgattg tccttaaaat
     4441 acgcaaaaga ggctccggga taaatccctg cctcgatttg ttcttctatt tttttaataa
     4501 tcttggtcca cttcatactt cttcaaacca caattcaaca ttatttctat ctttaccaag
     4561 gaagaatttt tcagacttag gaataaaata ttcagtagac tcaaacttct ggcgaagact
     4621 tgctatgtct aattcattga ccaagaactt gagcatagat aagtcccagg taaccgtatt
     4681 gtccacagtc aaatcctgcc cctgagctgg aataaaatca agggatgccc caatttcaga
     4741 tgattccaag aaactttcga tatcagttgg gagatgtaat tccatagaaa tctcaaattt
     4801 acttaaagaa attgatgcca aatctgtttg aaattcaggc ttttctccta cttctactag
     4861 acttgctctg tcatcttccg catgaatcaa aatcaaatca tcttctggtg agaaaatttc
     4921 aaaagcgtag ccattttgac ctttatataa tcgatgaatc gaatctgttt tagataagat
     4981 tccttcaatt tctaagggat tttccacctt gacaatcaat ctagctagtt tttttcttcc
     5041 ctctacctta cgagtacgca tactgggagc ttcttctaaa accagctttt caagacccgt
     5101 ttggtcacct agtgacagaa aggccgattc ttctaacaag gccttcattc caagggtttc
     5161 aatataaaat gtttcattta attttctatt attagctttt aaagtaggaa taatccgtac
     5221 aatctgattt acattcataa attcctccgt cactttttat tttaaaggat tttagaattt
     5281 tttacaagat attatgctca aatttcacat tatttagcta tttagtaaag atagtctttc
     5341 aaggcttttg acaaagcctg ataaccagca atactgaggt gcagtccatc agttgtatat
     5401 tcttttttga gttggcctgc ttggtctgtc aaacaatcaa atactggcac aaattccacc
     5461 tgcatatagg cagatgcaag atcttgatag gcttgattcc agttctgaat tttttcattc
     5521 gagcggatat agactgcctg ctgatactcc tctccctcat tgacaggtaa aatggaaagc
     5581 aatttaatct ctgtcaatgg ataatcgcga gcaacggatt gaatgatagc ttcgagatta
     5641 ttgagagcct cattcacagg aacatccttt ccgatatcat ttgtcccaat cagaagaaaa
     5701 attttatcta ctgctccacc atatagatga gcatcaaggt tctctagtaa cagtcctgtc
     5761 tgatagccac gaattcctcg attgacaatc atttttgaag tcccaaatag ctcctgtaga
     5821 ggataatact cgacaatgga atccccaata aaaagaatgt ttggttctac aacagaaatg
     5881 tgatttaggt gacgatactt ggtttgaatt ttttcttgtt cctttaggag ccaattttct
     5941 aataactgta ctgccacttc attctccttt ttctaaccag ttttccaaga cttgataaac
     6001 tcctccttgg ctattggctg gtgctagagc agtcgccaca gctttggctt tctcatcagc
     6061 attttccatc gcataggcaa ttccagccat ttcaagcatt tcaacatcat tttcactatc
     6121 accaaaagcc atgatttctt gggatttcaa gtcccagcgc ttgagtaatt cctccaagcc
     6181 ccatgcttta tgaatcccag cttggaggat atcaatgcaa ccatagccac tggatacagc
     6241 tcggacacgg ccatcaaaga gagcattgat ttcttccaaa accgaactca aacgttcctc
     6301 accaacaacc atgctcatct tgagcacacc accaaagagg tcagatgtta attcatccac
     6361 aaattgcatc cgttggtaga atttttcaat catttctgga gtcataaaac tttcaagatc
     6421 tgtaaaaatc gtaccttcct tgacaaaatc acccttcatc cccgttacaa caaactggtc
     6481 ctgacacgct cgacccttga aatgagccaa agccttgttg acaatggcgt catcccatgt
     6541 ctgagcctga atcaattcat tgttttcaaa aatacgagcg ccattagcaa caaccagaac
     6601 cactcgatcc accaagggac tcagtagttg tctcatgcgg tgaatttcat tgcccgtcgc
     6661 aatgacaaaa cgaatgcccc tttgatccaa ctgatctaaa atcttttcca atcgtgggag
     6721 atcaagctga cctctagcat ccagcaaggt cccatccata tctgtcgcaa ttatcttaat
     6781 catcatttac tttcctctgg attcaagcta ataccacttc aatcccacat gttcattcat
     6841 ttctttatag gctgtatcaa ttcgttcctt agtcgcttca tctaactggt cacgatgact
     6901 gccaccctct ataaaatctt ccaaaatata agtttggtaa tgatacaagg gatatccttc
     6961 tggtgaaaag gttccctttg gtgttcgatt gacccaagta ggatgagctt tagctgttcc
     7021 gatagttgtt tttccatcct tcttcttgat ggtgacatcc atgagaacac cacgttcagt
     7081 ccacttagca ttctcttcat ctcccataga ttcaatccgt tgattggaaa tgaagttccc
     7141 catttaatag ataatgagtt tcttatctcc atctttttca accgtttcag atggttcaac
     7201 aacgtgagga tgccctccaa agataatatc cgctccccaa tcgatcatct tgtgataaag
     7261 agctttttgt tcttcagttg gttccaatcg atactcaaca cccatctgaa gcatgataat
     7321 ggtgatatct gcttccttct ctgcccgttc aacttcagcc ttcatcttat cttcgtttaa
     7381 atctaaaaga taacgattat agtcttcctg agaaatatac tgctcaattc cattgaaacc
     7441 ataggaatag gctaacaatg caaccttgat accattcact tccttaatga ccagcggagc
     7501 ctgatcacgt tgttcgtgcg tataaactcc gattggagtg attccagctt tctcaataat
     7561 atcggccgtt gaaataactc cctcaatttg cgaatccaaa atatgattat gagctaaatc
     7621 cagcacatga taacctgctt ccttaatagc atccataact tcagcaggag cattaaagag
     7681 aagataaccc gctaaataat gatccttatt aatggttcct tcgaaatcac caatagctaa
     7741 atctgcttgc ttgagccaag gagtcacata ctcaaaattt tcatgaaagt cataggtacc
     7801 gtcttctttt ttagctgaaa agaaaagtcc atcgtggtag agcaaatccc cgtgggccat
     7861 aattctggca gttttttcct cttcctactc ctgagacttt tgcttagtcc cctcttgaga
     7921 aatagtatct cgtttttgac tagtcaaggg attccttgga agctttcaaa caacaagacc
     7981 aagcccattg ataaaccaac tgctagcaag agtatcgcca caaatccctt attgctccac
     8041 ttgcgataac tcctaaaaag tttaccaagc ccttcataaa acgaaaagct aaaccaccct
     8101 gatttcgatt ttgtcttctt tgtatcttcg ttctccctac tttcttatgc aagccttttc
     8161 tttttattat atcacagata agtatttctt tcacaattga attgaacttc ctatctattt
     8221 tctataaatc ctaaatgcca taatgctttc aattcttgtc attttgtgat atcatgtaga
     8281 ggaaatgaac taatccacag tggcttattc caagtatacc acttgggctt tggcagtagc
     8341 taactgcact aaacataata taaggagaga aaatgtctgc aatagaacgt attacaaaag
     8401 ctgctcactt aattgatatg aacgatatta tccgtgaagg gaatcctact ctacgcgcga
     8461 ttgctgagga agtcactttc cccctatctg accaggaaat catcctaggc gaaaagatga
     8521 tgcaattcct taaacattcc caagatcctg tcatggctga aaaaatggga ctccgcggtg
     8581 gtgttggact ggctgctccc cagttagata tctcaaaacg cattatcgct gttttggtac
     8641 ctaatattgt tgaagaaggc gaaactccac aggaagccta cgatttggaa gccattatgt
     8701 acaatccaaa aatcgtctct cactctgttc aagatgctgc tcttggcgaa ggagaaggtt
     8761 gcctgtctgt tgaccgtaac gtgcctggct atgttgttcg ccatgcccgc gttactgttg
     8821 actactttga caaagatgga gaaaaacacc gtatcaaact caaaggctac aactccattg
     8881 ttgttcagca tgaaattgac cacattaacg gtatcatgtt ttacgatcgc atcaatgaaa
     8941 aagacccatt tgcagttaaa gatggtttac tgattcttga ataaagaaaa tcccgttgca
     9001 agacggggtt ttgtgttata atagaggcat gaaaacaaat gatattgtct atggcgtcca
     9061 cgccgttacc gaagccctcc ttgcaaatac aggaaacaaa ctctacctcc aagaagatct
     9121 ccgaggtaag aatgttgaga aagtcaagga actagctaca gaaaagaagg tgtccatttc
     9181 ttggacatca aaaaaatctc tctctgagat tactgaaggt gctgttcatc aaggttttgt
     9241 tctacgagtg tctgaatttg cctatagcga gctagattac atccttgcaa aaacacgcca
     9301 agaagaaaat ccacttctat tgattctaga tggtctaacc gatccccata atctaggttc
     9361 tatcttgcga acagccgatg cgaccaatgt ttcaggtgtc atcattccca agcaccgtgc
     9421 tgtcggagta actcctgtcg ttgccaaaac agccacaggt gctattgaac acgttccaat
     9481 tgcccgagtg accaacctca gtcaaacctt agataaactt aagaatgaag gtttctggac
     9541 ctttggaacg gatatgaacg gtactccttg ccacaagtgg aatacaaaag ggaaaatcgc
     9601 cctcatcatt ggaaatgaag gaaaaggtat ctctagcaac ataaaaaaac aggtcgatga
     9661 aatgattacc attccgatga atggacatgt tcaaagcctt aatgccagtg ttgctgcggc
     9721 cattctcatg tacgaagttt tccgaaatag actataaaaa agtttccagt catctgattg
     9781 gaaacttttt tatgattaac tatgttctgt aatgaattta taggcttctt gaccagcgat
     9841 agctccatct ccaaccgctg ttgttacttg gcgaaggtct ttcaagcgaa catctccaac
     9901 tgcaaagata ccgtcgactg cagttttcat gtggttatct gtcacaatcc atcctgcctg
     9961 atcttggata ttcaattctt taacaaaatc gccaagaggg tccaatccaa catagataaa
    10021 gacaccaccg aagacttgtt ctgtcacttg acctgttttc acattttcaa atacgactga
    10081 ttctactcgg ttttcaccct tgatttccct tactacagaa tcccagataa agctgatttt
    10141 ttcattcgca aaggcgcgat cttgtaaaac cttttgggca cgaagttggt cacgacggtg
    10201 aacaatggta acagtcttag caaaacgagt caagaagagg gcttcttcaa cagctgaatc
    10261 tccaccacca actaccaata aatcttggtc acggaagaaa gcaccatcac acacagcaca
    10321 gtaagaaaca ccacgactgt tcagttcttc ttctccaggt actcccaaag gacggtgttt
    10381 agaaccagtt gctacgataa ctgtacgtgt ttcatatgtt tggtcatcag tcatcacttt
    10441 cttaaaatca ccatggtctt cgacattttc aacataacca taaatgtgct ccacaccaag
    10501 attttcaagt ggttcaaaca tcttttcagc caattcaggt ccactaatat tagcgtatcc
    10561 tgggtaattt tcgatatcag atgtattatt catctgacca cctggcagac caccttcaat
    10621 caaagctact tttagattgc ttcgagcagc atacaaggcc gcagtcatcc ctgcaggtcc
    10681 agcaccgata ataatagtat cgtacatata gattccttct ttcttgttgt aactatcttt
    10741 attctaactc tttcttgtca atcacgcaag gattatgcct tgaaaacaag aattaaactc
    10801 ataaccgcaa aggataatac tgggacaacc aagactccaa tcataatcat atcatagaga
    10861 tgggcttggc gagccctggc tgtcttatca actcccgaca tggctctcag accaatccaa
    10921 atccctaaaa aaatcagaac aaggatggtg gtcaagatca aactctcgaa atataaagaa
    10981 aatagttgca gtagcatgat ttctctcatt tctatctttt ttaaagagta aactcagcta
    11041 gtccaactaa ctgagttttc ctttatctat tatatcaaat ataagtccgt ttgtaactag
    11101 cgaagaattc ttttgtccgc tcttctttag gggtgtggat aatctcatcc ggtgttccag
    11161 actcgatgat tttcccctta tctaagaaga gaatcttatc agccacttgg gctacaaagg
    11221 acatgtcatg actaaccaaa atcatggttt gacctgactt agcagcatct gcaatagact
    11281 tttctacttc accaaccaat tctgggtcaa gggctgaagt tggttcgtct aagagcaaaa
    11341 catctggttt catagcaagc gcacgcgcta gggcaacccg ttgcttctgt ccacctgata
    11401 aatggcgagg ataatggttt tcacggtccg aaagcccaac cttagccaac tcttccttgg
    11461 caatcttagt cgcttcttgg tcagataatt tcttgacaac aaccaagcct tctttcacat
    11521 tatcaagtgc tgttcggcgt tcaaacaaat taaactgttg gaaaaccata gacaacttac
    11581 gacgtagggc aaggatttct tcttgagtga ttttagaaaa atcaactgaa aaaccatcaa
    11641 tctgaataga gccactgtca ggtgtttcaa gataattgag actgcgaaga aaggttgatt
    11701 ttccagctcc tgaagaacca atcaaggcta caacttcccc tttttgaata tccaagtcca
    11761 gatgatccaa gacagtctgt cctgaaaagg atttgcttaa atttgaaatc ttaatcatta
    11821 acgaaggtct cctttcacat ctgtttgcac tgtatcaggt gcagaaatag ccatttttct
    11881 ctcgatgaaa cgaccgaggc tttcaattcc gatattgacc acccaataaa caagggcaac
    11941 ggagatgaag cgttcaaaat agcgataatc agctccacct agaatctgag cttgggcaaa
    12001 gacttccaca acacccgcac taaaagctag agatgttccc ttggtcaaac cgatgaggga
    12061 attaatcaag gttggagtag ctaccaccgc tgcattagga ataatcactc gtcgataaac
    12121 ttgcgctcgg gtcataccca gactgcgtgc cgcctcaatc tcaccaggat taactgagag
    12181 aatggctgca cgaatggttt cactagcata agctgcctca ttaaaggcaa aggcgacaat
    12241 cgcaaaagct gcagctggaa tcgcattgat attgagacca gttccccatt gctgattgag
    12301 ggctttcaaa gccaaaggga ttccgtagta ggtcaacatg agttgcacca aaatcggtgt
    12361 cccttttaag aaactaacaa agaaggcctg caagggatat aaaatcttga cacgattgat
    12421 cttcacaatg gcaaaaagaa gcgccaaaac caagccaaaa agggcaccac caattgtcaa
    12481 cataattgtt gttggaagtt gttggacaat tctagggatt ccatcaaaga ccgaacgtag
    12541 gctaaacagc ttgccatccg gaatcaattg catcaagttt tggtaccaat ctgatgctaa
    12601 aatcgttgta acattcataa aaacatcctc ttctgataat gattcattct accatgcttc
    12661 tgccgtcaat gaaattattt gctacggtca gatacagact ttgtctacct actagtttat
    12721 catactttga aacagggaag ttatatattt tatctatcaa agagatagat aaaaactatt
    12781 tcagtcccag ttcttcataa gattttcgat aaccgatttg cttaacagtt ccattttcta
    12841 ctaaaatagg ccgttttaac aacataccgt cacttgctag caactcagcc gcttcttggt
    12901 ttgacaaact tcctacctta tcttttagcc ctaattcacg gtatttgata ccactggtgt
    12961 tgaaaaattg cttcaattca aatcctgagg tttctagcca attcaaaatg acttcttggc
    13021 taggtgtttc ttccacgata tggacggctt tatagtccac acctaattga tttaattctt
    13081 gttttgcttt tttacaagtt gaacatttgg ggtattcgat aaattctaac atgtgcttct
    13141 ccttctattg tttctatctt taaaatctac tccttcatgc tccaagagcc aagctttctt
    13201 ttccactcct gcagcataac ctgtcagacg cttgcctgct cccaacacac gatgacaagg
    13261 tactaggata gaccaaggat tgcgtcccac tgctccacca attgcttgag cagaagccac
    13321 ttgcaggtct tgagcaattt gtccataggt cactgtctga ccataaggaa tgccctgtaa
    13381 atagtcccaa actctctttt caaaattcgt tccgattggc gccaagagca agttggataa
    13441 atcctgaggc ttgcctttaa agtaatcatc taagcaagca ataactgggt ctaaaatagg
    13501 atgactaaca acttcttcta tcgtttcatc tcctagtccc ctctcaaaat gcttctgctc
    13561 ctgaacccaa attccataca aataatggtc atcagctaca agtgatagaa taccaattgg
    13621 tgaggagtag aggattttta catacttctt ttgcattatt tctttaattt ttgataggcc
    13681 aagcgttcgc catctactgg caccttacca acttgtttaa aaccaagttt ttcaaaaata
    13741 tgttgcataa ccttgttttc agcatgcgta tctgagcgaa aatcaagata atcaaaacct
    13801 tcaatcaagc cctctaagaa ggtttgagca actccttttc cctgcacatc tgctgccaca
    13861 gcaatacggt gaaagactag atactctgac tctccagctt gccagtttcc ctcataaata
    13921 gcttcatagg cctcctctgg actcttggtc acagcagcat aggctagcag ttctccctct
    13981 tccaaggcta cgtaggcttg ccctgagatg atatcatcga taataacatc tgcatttgga
    14041 tagccatttt gccactggtt actaccagca tctgctaaac attttttggc atcctctatc
    14101 acctgcatga tggcatctac ttcatttgga aaagctaaac gaatttccat cttttttcct
    14161 catttctgac tagtttctcc catcatagca taatttaagt ctttctacaa gcagttaaaa
    14221 cttgactttg tatttttgtt attttataat ctagttatca aaactatata aaaaggagtt
    14281 gaaaatgaaa actacctttt cctacccaaa atgggcagaa attccaaaca ttaacctcta
    14341 tctggaccag gttttgctct atgtcaatca ggtctgcgcc cctatctctc ctaataaaga
    14401 caaaggccta acagcatcta tggtcaacaa ttatgtgaaa aatggttacc tgacaaagcc
    14461 tgacaaaaaa aaataccaac gccaacagat tgcccgtttg attgctatca caaccctcaa
    14521 gtctgtattt tctattcaag aaatagccca gatacttaat actctacaaa ctcaagcaag
    14581 ttcagaccaa ctctacgatg cttttgtgga ctacatgaac caaggaattg acccagctaa
    14641 ccctattatc caaaccagct gccaaaccgt taaactctat catcaaactc tagacttaat
    14701 cgatcatact caagaggagg taatccaatg aacactagtc ttaaactcag caaacaactc
    14761 agttttggag aggagattgc taatagcgtg acccatgctg tgggtgcagt catcatgctt
    14821 atcttgctgc ctatttcatc catctatagt tatgaagcac acggattttt atcctctatc
    14881 ggcgtttcca ttttcgtcat cagtctcttt ctcatgttcc tatcatccac catttatcac
    14941 tctatggcct atggttcgac ccacaaatat gttttgcgaa tcattgacca ttctatgatt
    15001 tacgttgcca ttgccggctc atacacgccc gttgtcttga ccttgatgaa taactggttt
    15061 ggctatctga ttattgtcat ccaatgggga acgaccatct ttggtattct ctataaaatc
    15121 tttgctaaaa aggtcaatga gaaatttagc cttgctcttt acctgattat gggctggttg
    15181 gttctggcta tcattcctgc cattatcagt caaacgacac ccgttttctg gagtctcatg
    15241 gtaactggcg ggctctgtta tacagttgga gctggatttt atgccaagaa aaaaccttat
    15301 ttccacatga tttggcatct ctttatccta gctgcgtccg cacttcaata catcgctatt
    15361 gtttattaca tgtaaaaaag ttgagaaatt caatctcaac ttttttcttt acacatattg
    15421 ataaagtact ggtgcaagcg cacatcatca gtcaattctg gatgaaaaga acttaccaac
    15481 atattttttt cttgggctgc aacaatttga ttgttcactg ttgctaaaat ttctacaccc
    15541 tcaccaacac tactgataat cggaccacgg ataaaggtca ttggaatctt gccaactccc
    15601 ttacattctg cttccgtgta gaaacttcct aattggcgcc cataagcatt acgctcgacc
    15661 accatatcca tagttcctag atgactctct ttctgagaag tgatttcctt agccagcaaa
    15721 attaagcccg cacaggtccc aaacactggt aagccagata gaatggcttc tcggatggga
    15781 agtagcatgt tctggtcacg taagagcttg cccatggttg tagactcacc accaggcaaa
    15841 atcaaacccg acaagtcact ctgatcttgc tgaaaatcat ctagatttct gagttctaca
    15901 ctctcgacac ctaattgatc tagcactttt gcatgttctg caaaggcccc ttgcaaggcc
    15961 aatattccga ttttcatcta ttttcctcgt tcagccatga gaatttggat ttcattttca
    16021 ttaataccaa ccatggcttc tcctaaatct tcagagattt gagctagaat ttgaggatta
    16081 cggaagttag tcacagcctt aacaatggca ctcgctcgtt taacaggatc tcctgacttg
    16141 aaaatacctg aaccgacaaa gaccccctct gcccctaatt gcatcattaa cgcagcatct
    16201 gctggcgttg caacacctcc agcagcgaaa tttacaactg gcaattttcc atgttcatga
    16261 acatattgga ccaattctac agggacttgc aaatccttgg cagcaacata aagctcgtcc
    16321 tcacgtaagt tttgaatgcg gcgaatttcc tgattcatca tacgcatatg acgaacagct
    16381 tggactatat cccctgtccc tggttctcct ttggtacgaa tcatggaagc accttcagcg
    16441 atacgacgca aggcttcacc caaatcctta gcaccacaga caaaaggaac ttggaattct
    16501 ttcttgtcca catggaaacg gtcatcagct ggagatagaa cttcactctc gtcgatataa
    16561 tcaatttcaa tagcctctaa aatctgagct tcaacaaaat gcccgattct gaccttagcc
    16621 attactggaa tactaaccgc ttcttggatt tccttaatca tctttgggtc gctcatgcgg
    16681 gaaactcctc cagctgcacg aatatcagcc ggaattcgtt ccaaggccat cacagctgcc
    16741 gcaccagcag cttctgcgat acgagcctgt tcaggattct gcacatccat aataacacca
    16801 cccttgagca tctgtgccaa gtttttattt agttcataac gattttcagt catttgtttt
    16861 atcctcatca tttgtattgg tagctacctt ttcattttaa gtcagaacag aatgaagcac
    16921 aagataaaaa aactataatt gtatggggac agattagcta aaaactatct gaccttaact
    16981 taaggccaga tagctcattc atttttattt ttcagccgta agggcagcca ttgtgatgta
    17041 gttgtatggt ttgttgaagt gtggcaagaa gaagaggtct gtcaatgcca atttatcaat
    17101 tgtcacatgc tcttggatag caagtgagaa catgtggatt cccatgctaa ttgcaatatc
    17161 atgtgaaacc atttgggcac caagaatttc acggctatct ttgtcaaaga caatcttaat
    17221 tgctacttca tggttgtcat gtttcatgaa ttctggtttt tgaagatcgt taaagcctgt
    17281 ttcagttgcg ttgtaaccag cagctttcgc tttttcaaga gtcaaaccag ttgaaaccat
    17341 gtgaagaccg tagattgaga taccatttga accttgaaca ccgattcctt ccaattcatg
    17401 tccacaagca ttgtaggcac caacgatacc agtgcgaaca gcatttgaag caagagcgat
    17461 atagcttgta tctttacgag cattgtcata aacagtcgca cagtcaccaa cagcgtaaac
    17521 gcctgggata gatgtttctt gtttcttgtc tacaaggaag gcaccgttgc ggaagagttc
    17581 gatcttacca tctgcaaggg ctgtgtttgg acggaaacca actgcaagga taaccatatc
    17641 cacgtcaaag ctttctttgt cagtaatcaa gcgttcaact ttaccgtcac cttcgattgc
    17701 tttaacagtt tgacctagag ccaagcggat gttgtgatct tccaagttct tcgccatcat
    17761 ttgtgtgaag tctttgtcat agtaaccgtt caagacagta tcaacgatat caacaaggac
    17821 aacttctttt ccaagacgtt caaaggcttc agcaagttca acaccgatgt aaccaccacc
    17881 aacaacggcg atacggtcga ggtgttggct cttgtcagaa agtttattga taacttcttc
    17941 agcattttgg tacaatttca cgaattgtac gttttcaaga gttgctttaa attcgcggtt
    18001 tcctttaaca atttcaacac cttcgattgg tggcaagatt ggtgtagaac ctgtagcgaa
    18061 aatcaatttt tcgtatgatt ctttgtgctc ttttccttca acttccgctg taactacttt
    18121 gttatcatag tcgattgaaa gaacaggtga gttcatgtaa actttagcac ctttagcttc
    18181 caatttttct ttatcagaat agaacaagcc ttcagcaccg tcaatttgtt caccaatcca
    18241 aagagccatt ccacatccta ggaaagagat gttagagttt tggtcaaata caacaatttc
    18301 gttctcattt ccaaaattat ccaacatggt attgatacat gctgtaccag cgtggttagc
    18361 accgactaca acgattttac tcataaaaat ttcctgcctt cattctaaat ttaccttact
    18421 agtataccat ttaccgttag cgtttacaag gggggtacta ttatttcagc attttttatc
    18481 aaaaataatt ccccatttat cctgttttca agcctcatga attttatgag catatttctt
    18541 gactttttcc aaattttgtt tgtgaaaatt ctaactttat ggtattataa taacatgaaa
    18601 atgaaatgta agggcttcaa tttagatgat tatttgatat tacaacttat tttctataag
    18661 agaaaggagt tggtagcttg actcttcgtt cacattctga acggctaatg gggactacta
    18721 tcactatttc attagtggat gagcaagccg atatctttct ccaaaaatcc tttgacttgc
    18781 tcaaagaact tgaataccgc ttcaacgcca atagtcaaga atctgagttg atggaaatca
    18841 attatcaagc aggaatagcc cccgtcacgg ttcatccaga cctgtttgag ctgatttcac
    18901 ttgggttaga gcatagccta gcgctctcta gccacctcaa tatcagcatt ggtcccttaa
    18961 ttcaaacctg gcgtatcggt ttttcagatg ccaaggtcgc ccagcctcaa gaaattgaat
    19021 cggtgctgcc tctaatcaat cctcatggta tcgagttaga ttcttctact tctactgtgt
    19081 ttttaaaaca gaaaggaatg aagatcgatc taggttgttt agccaaagga tacagtgcgg
    19141 ataaggttgc ccaatttctt aggaaagagg gggtgacttc tgccttgatc aatctgggag
    19201 ggaatatcct gaccattgga aaaaatcagg caagagggga taacccatgg caaatcggga
    19261 ttcaagaccc agccaatcct aggggaaatc atttaatgac catccctgtt atcaataaat
    19321 ctgtcgtgac ttcaggcatt tatgaacgtc acctgaccgt cgatggacaa gattaccatc
    19381 acatttttga cagtcaaaca ggatatcctg ttgaaacgga actagcgagt ctaacaatca
    19441 tctctgataa atcagtcgat ggcgaaatct ggacgactcg actctttgga gaaagacctg
    19501 cttctatcct ctggcaagtc gaaagtttgg agggcatcga agtcatcctc atcgataaag
    19561 aaggccacct aagctgttct tcaggaattc ctactctata gagacaactg tttcaatgga
    19621 attttaaaat tgtattatgt aaaaagagaa aggaaatctc atgttaaaac ttattgctat
    19681 tgttggaaca aattcaaaac gttctacaaa ccgtcaattg cttcaataca tgcaaaaaca
    19741 cttttctgac aaagctgaaa ttgagcttgt tgaaatcaag gccattcctg tcttcaacaa
    19801 accagctgac aagcaagtac ctgctgaaat attggaaatt gctgctaaaa tcgaagaggc
    19861 agatggcgtt attatcggta ctcctgagta tgatcactct attccagctg ttttgatgag
    19921 cgctcttgct tggttgtctt atggtattta cccacttttg aacaaaccaa tcatgattac
    19981 aggtgcttct tacggtacgc ttggttcatc tcgtgcccaa ttgcaacttc gtcaaatctt
    20041 gaatgctcct gaaatcaagg caaatgttct tccagatgaa ttcttgctct cacactctct
    20101 tcaagcattt aacccaagtg gcgacttggt tgaccttgat gttatcaaga aattggatgc
    20161 catctttgat gacttccgta tctttgtaaa aatcacagaa aaattacgta atgcacaaga
    20221 attacttcgc aaagatgctg aagactttga ctgggaaaat ttgtaagata ggagaccaaa
    20281 aagaatgaaa tttgttggac ttgttggatc aaactacgat caatcatata accgtaagct
    20341 cttggaattt atccgtcgct acttcaaact caaatttgaa ttagaagttc ttgaaattga
    20401 cgaagttcca atgtttaacc aagatgaaaa atgggatgaa agtttccaat tacgttattt
    20461 atataacaaa attactcgtg cagatggtgt tatcattgct actcctgagc ataaccacac
    20521 tatctcagct tcactcaaat ctgtacttga atggctttca tacgaagttc atccatttga
    20581 aaacaagcct gttatgattg tgggtgcatc atactatgac caaggaacat cacgtgctca
    20641 agttcacctt cgtaaaatcc ttgatgctcc aggtgttaat gcctacacgc ttccaggtaa
    20701 cgaattcctt cttggtaaag ctaaggaagc ttttgataac aacggaaata tcaccaacga
    20761 aggcactgtt aaattccttg aaacttgctt agataacttt gttaaatacg taggagtcgt
    20821 ttcgaaattg aaaaaaccaa aaccaatcga atcagaagac ttggattgtg gaaaaccaat
    20881 cgctacaacc atcacagaag ttgaccctga cgatccagaa tgggtagaaa aagttgcagc
    20941 aatcactggt gctgtttctg gtgataccta tgtcaaattg gaccacggta tcctaacagt
    21001 taaccaaatt gatatgttct tgaaagctat gccatttgaa ttgacatacg ctgacgacaa
    21061 caaccaattc ctctactaca acaacgctca ccaagatcca gacactatgt tcgctaaacg
    21121 tgtaccacct caatcaggta gccgtatgtc gactgttcat ggttctcttc caccagcacg
    21181 tatgaagaac gtagagtggg ttatcggaac acttcgcaac ggaaaccaag aatacgtccg
    21241 tacgatcgtt ccaggttctc ctgcaggtgt tatcaacact cacaactacc aagcaatgta
    21301 ctatcctgat ggatcatacg ctggtatcaa tgaaatcgtc tttaacttcc aaccatggct
    21361 tgactggtac ctaaaagaga ctggtcaacg tttggtgggt ggtagcggac catttgctcc
    21421 tgctgccgga ggtcatggag acgccgatgc tacttctggc gcttctgatt caggggatgc
    21481 tggaggccac ggtggtgacg cagacgctac agccggtgca agttactaat gatagttttt
    21541 aggaaccatt atggttcctt ttttagcgaa aaaagactag caaacacaaa ctgctagtct
    21601 ctttgttatc gtttcatcta tctttgttaa aagaaagagt acttatactc aatgaaaatc
    21661 aaagagcaaa ctaggaagct agccgcaggc tgtacttaag tacggcaagg tgaagctgac
    21721 gtggtttgaa tttgattttc gaagagtatt attatgaatt tgggtcatca agactacggc
    21781 catgtaatcc tttttcacgt tgtacttggc gtagtttttc tggtgtaaca tcgtttcctt
    21841 cttcgtccac aattttgatt ccttcaatgt ggtgacgaac agcgcggcga taaccttcga
    21901 tgtactcctc acgtagtttg gcttgttcca ctttttcttc tgatgttaag ccttctgttt
    21961 ttttcttttt agcaagctca ttgatacgag caattttttt cggatccatt tcttctcctt
    22021 tgtaataaga taaagtccgt ttatcaaatt ttatttagtg ttaatttggt taaaacaagc
    22081 aaacttagct gctttcttgg ttagttgtga caagattgcc aaacgatttt gacggacagc
    22141 ctgatcttca gccattacca tagtattttc aaagaaagca tcaatgactg ggctaagcgc
    22201 aaagagttgt ttcaattgct gacttgcagg tcctgataaa acgagtgttt ctactgcttc
    22261 tgccaaagct ttttcttggt cattctcaaa tagtgctgaa tcaactgtag caaccccttc
    22321 tgccttctca gccaggttaa aggcacgaga aagtgattca acagatggtt taaaatcttc
    22381 ttccttgctt acttctacga gagcacttgc tgcttccaac atatctgcca caacaaagtt
    22441 tgaacctgca agaactgctt ccttgatatc ttttggagta gagcccatca tcttatcaac
    22501 acgagccttg ataaagtcca taacctctgc tttattttca taagtcaaac tgtcaaattt
    22561 caatgcataa aggctatcaa tcagctcatc catagcaatg tgccaaccaa aggcatccaa
    22621 gatacgaacc acaccttgag ttgcacgacg aagggcataa gggtcattag aacctgatgg
    22681 aatcaatcct actgagaaga aactcaaaat cgtatccaat ttgtctgcaa tggctagaac
    22741 tgcgccgacc ttgctctctg gaagttctcc ttcagctgat gtaggcatgt agtgttcacg
    22801 aatagcagct gccaccgctg gagtttcacc agcaagaagg gtgtattttt cacccataat
    22861 tccttggagt tcgtcaaatt caccaaccat acctgtcaac aagtcaaact tgtaaatggc
    22921 tgctgcacga gcaaggtcaa ctgtttcatc cactgacaaa ccagcttttt ctgccaaaag
    22981 tacagtgatt tgacccgtac gaatcatgtg ttcacgaagg gaaccaatct tctcatggaa
    23041 ggtgacattg tttaattttt caacaagatc tgaaatcacc aatttttggt cttcacgcca
    23101 gaagaattct ccgtcttcca agcgggctac caagactttt tcatttcctt tgatgacatt
    23161 tttcaaacgc tctgcgtttc cgttacgaac agaaatgaag tttggcaaga gttttccatc
    23221 ttgatcacga acaacaaagt aacgctggtg ttccttcata gaagtcacca agacttcttc
    23281 tggaacttca aggtatttag catcaaaact tcccatgaag gcagttgggt attcaaccaa
    23341 attcaagact tcattcagca aatccgcatc aatttcgata cgtacaccat gttttgcctc
    23401 aatttccttg atttggtcaa caatcatttg ctcacgttca catggatctg cgattacaaa
    23461 ctgcttacga aggtcttctt catagctcaa tgctgactga attttggttt ctttccccaa
    23521 gaaacgatgg ccacggctca cacgacttcc cttgatatca aggaaatcca agtcaaactc
    23581 ttgctcatcc aagagaacag ttaaagtgtg aacagggcgg atgtattcaa agctatttcc
    23641 agcccagtgc atgctgacag ggaaagtcaa tgacttcaag acatctacaa caccaggaac
    23701 aatggcttca actgcttgac caacttcttc cttagtgaca tagacatatt cttcaccctt
    23761 gatttcacgg aattcgatat cttcaacagt caaacctttc ccacggacaa atccttgagc
    23821 tgctttggtg aagtttccat cactatccaa ggcaattttc tttgctggtc ccttgaaatc
    23881 ttctgtcaaa tcagactgtt tgtctgataa accagtcaca cgaacagcca aacgacgtgg
    23941 tgttgagaag gtttgaatgg cttcaaaaga caggcggttt tccttgagga aggctgccat
    24001 tttttcgcct agttgttttt cacttggtgt gacaacatag gctggtaatt cttcaagacc
    24061 gagttctact aataagtttt ttgtcatgtt ttttctccga aaagtatctt tttattttcc
    24121 agtttgcctt atgtgattgg cacgcaagca accaacttcg ttgtttcatt gctaaaattg
    24181 gagccaaaag tctccaattt tgcctagtat ccttagtttc actaaggata ccttcatcac
    24241 tgcggcaact ggctcagggc tcactctgtt cgcccatttt cgtaatttgt cactctcttt
    24301 attctgcgtc ttctgctagg agtttagctc ttgtttcttc atccaaaagt gggtagccta
    24361 ggcgtttgcg ttctgcgaca aaggttttgg ctacgacacg ggccaagtta cggatacgag
    24421 cgatatagcc tgcacgctct gttacagata cggcaccacg cgcgtcaagc agattaaagg
    24481 tatgtgaaca tttgagaaca tagtcatagg cagggtgtac caagccttct tctaatgcac
    24541 gaccagcttc tttttcaaac ttatcaaaat tttcaagcaa catttcttgg tccgaaattt
    24601 caaatgaata ttttgagtgc tcatactcag gctggataaa gatttctccg tattttacac
    24661 catcagccca ctcgatatca tagacagagt caacttcttg aatgtaagaa gccaaacgct
    24721 ccaaaccata ggtaacttcc gcagtcacag gaccagttgc caatccaccg acttgttgga
    24781 aataagtaaa ctgagtgatt tccatcccat caagccaaac ttcccaacca agaccagctg
    24841 aaccagtcga tggattttcc cagttgtcct caacgaaacg aatatcgtgt tccaaaggat
    24901 tgatacccaa tttttccaaa gactcaaggt aaagctcttg gatatttgat ggagaaggtt
    24961 tcataaccac ttggaattgg tggtgttggt aaaggcggtt agggttttcc ccataacgac
    25021 cgtcagcagg acgacgtgat ggctctacat aagccgcatt ccatggctca ggtccgatag
    25081 cacgaaggaa agtgtaagga ctcattgttc cagcaccttt ttcgttgtca taagcctgca
    25141 taagcataca accttggtca ttccaaaatt gttgcaaagt aaggatgatt tcctggaaag
    25201 tcaatttttt agacattatt tactcctttt aattaaaaaa atttgttttc tttatttcat
    25261 tttcttttaa aactattatt gagaaattat ttctcttttt ttgcatgttt atcaatttat
    25321 caatactaca aaagacttca tttgttgtta caagtattag ttttttatat ggttttctat
    25381 tagtttcaat tgaatagtta tccgataatt catataattt cgtaatatac ctatctaaca
    25441 ctgaactccc aatattattt tgcaaatatt taatctcaaa gataatatct ttatgtaaca
    25501 tatcttgtcc aatcgctatt acatcatata aaaatctatt tagaatacta tctgaaacta
    25561 ttttatagtt cactggcgaa gtctcataga ttcttctaac tacactgttt tcaatagatt
    25621 ggtaatattt tattttttca gaggtttctt cagtaagttg ttctaatttt aaatcacttt
    25681 ttatactttc ttcaattttt tggggagatg ccttagtaat tcttttatta atttcagaga
    25741 tttttaaagc cttttcttca taatctaaat cttcaaactt tgtccaatca cttaacccat
    25801 tataaattag aaaaattcct attagtatac agaataccag caacaataat gaaaa
//
LOCUS       DAYYQN010000027        25542 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYYQN010000027 DAYYQN010000000
VERSION     DAYYQN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.027"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..636
                     /locus_tag="ACHBRQ_001061"
                     /pseudo
     CDS             1..636
                     /locus_tag="ACHBRQ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(843..3101)
                     /locus_tag="ACHBRQ_001062"
     CDS             complement(843..3101)
                     /locus_tag="ACHBRQ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9592751.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(3330..3560)
                     /locus_tag="ACHBRQ_001063"
     CDS             complement(3330..3560)
                     /locus_tag="ACHBRQ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HFM9592752.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3750..4433
                     /locus_tag="ACHBRQ_001064"
     CDS             3750..4433
                     /locus_tag="ACHBRQ_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592753.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4433..5167
                     /locus_tag="ACHBRQ_001065"
     CDS             4433..5167
                     /locus_tag="ACHBRQ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9592754.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            5321..6178
                     /locus_tag="ACHBRQ_001066"
     CDS             5321..6178
                     /locus_tag="ACHBRQ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9592755.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            6315..7136
                     /locus_tag="ACHBRQ_001067"
     CDS             6315..7136
                     /locus_tag="ACHBRQ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HFM9592756.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            7150..7989
                     /locus_tag="ACHBRQ_001068"
     CDS             7150..7989
                     /locus_tag="ACHBRQ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9592757.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMSFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            8287..8970
                     /gene="rpiA"
                     /locus_tag="ACHBRQ_001069"
     CDS             8287..8970
                     /gene="rpiA"
                     /locus_tag="ACHBRQ_001069"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9592758.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            8984..10195
                     /locus_tag="ACHBRQ_001070"
     CDS             8984..10195
                     /locus_tag="ACHBRQ_001070"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9592759.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            10197..10757
                     /locus_tag="ACHBRQ_001071"
                     /pseudo
     CDS             10197..10757
                     /locus_tag="ACHBRQ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            10754..11563
                     /locus_tag="ACHBRQ_001072"
     CDS             10754..11563
                     /locus_tag="ACHBRQ_001072"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9592760.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            12039..12434
                     /locus_tag="ACHBRQ_001073"
     CDS             12039..12434
                     /locus_tag="ACHBRQ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HFM9592761.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            12444..13019
                     /locus_tag="ACHBRQ_001074"
     CDS             12444..13019
                     /locus_tag="ACHBRQ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HFM9592762.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            13044..13556
                     /locus_tag="ACHBRQ_001075"
     CDS             13044..13556
                     /locus_tag="ACHBRQ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HFM9592763.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            14214..14924
                     /gene="deoD"
                     /locus_tag="ACHBRQ_001076"
     CDS             14214..14924
                     /gene="deoD"
                     /locus_tag="ACHBRQ_001076"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9592764.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(14947..15471)
                     /locus_tag="ACHBRQ_001077"
     CDS             complement(14947..15471)
                     /locus_tag="ACHBRQ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HFM9592765.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(15524..15760)
                     /gene="rpsT"
                     /locus_tag="ACHBRQ_001078"
     CDS             complement(15524..15760)
                     /gene="rpsT"
                     /locus_tag="ACHBRQ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9592766.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(15828..16748)
                     /gene="coaA"
                     /locus_tag="ACHBRQ_001079"
     CDS             complement(15828..16748)
                     /gene="coaA"
                     /locus_tag="ACHBRQ_001079"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9592767.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            16938..17342
                     /locus_tag="ACHBRQ_001080"
     CDS             16938..17342
                     /locus_tag="ACHBRQ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592768.1"
                     /translation="MKILKRYILELCFILSFALPFTKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSILIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            17549..18139
                     /locus_tag="ACHBRQ_001081"
     CDS             17549..18139
                     /locus_tag="ACHBRQ_001081"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9592769.1"
                     /translation="MSKMYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDF
                     GSQLLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEK
                     NNAKATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            18136..19413
                     /locus_tag="ACHBRQ_001082"
     CDS             18136..19413
                     /locus_tag="ACHBRQ_001082"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9592770.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            19431..20093
                     /gene="deoC"
                     /locus_tag="ACHBRQ_001083"
     CDS             19431..20093
                     /gene="deoC"
                     /locus_tag="ACHBRQ_001083"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9592771.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            20080..20469
                     /locus_tag="ACHBRQ_001084"
     CDS             20080..20469
                     /locus_tag="ACHBRQ_001084"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9592772.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            20559..21611
                     /locus_tag="ACHBRQ_001085"
     CDS             20559..21611
                     /locus_tag="ACHBRQ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HFM9592773.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            21755..23290
                     /locus_tag="ACHBRQ_001086"
     CDS             21755..23290
                     /locus_tag="ACHBRQ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592774.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            23283..24341
                     /locus_tag="ACHBRQ_001087"
     CDS             23283..24341
                     /locus_tag="ACHBRQ_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592775.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALPHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KEFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            24344..25300
                     /locus_tag="ACHBRQ_001088"
     CDS             24344..25300
                     /locus_tag="ACHBRQ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592776.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            25385..>25542
                     /locus_tag="ACHBRQ_001089"
     CDS             25385..>25542
                     /locus_tag="ACHBRQ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9592777.1"
                     /translation="MKLSCDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKG"
BASE COUNT         7467 a         4561 c         5762 g         7752 t
ORIGIN      
        1 acaatccaga cgcttatttg actgaaatag cttctgaatt tggctgtcat ctaactacca
       61 tccactatgc gctcaaagct atgggttaca ctcgaaaaaa agaaccacac ctactatgaa
      121 caagacccag aaaaagtagc cttatttctt aagaatttta atagtttaaa gcacctagca
      181 cctgtttaga ttgacgaaac aggattcgat acttattttt atcgagaata tggtcgctca
      241 ttaaaaggtc agttaataag aggcaaagta tctggaagaa gatatcagag aatttctttg
      301 gttgcaggtc taacaaatgg tgagttaatc gctccaatga cttacgaaga gacgatgacg
      361 agcgactttt ttgaagcttg gtttcagaag tttctcttac caacattaac cacaccatcg
      421 gttattatta tggataatgc aagattccat agaatgggta agttagaact cttgtgtgaa
      481 gagtttgggt ataacttttt acctcttcct ccctactcac ctgagtacaa tcctattgag
      541 aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat taccaagttg caataccttt
      601 tatgaggctc ttttgtcttg ttcttgtttc aattgactat agcagattga aataagatga
      661 gaacaaatcg attgggaaag taaaattaat ttctataaat gttttagcaa ttgtttcgta
      721 ctatttcaga ttcagtctac tatatatcat aatggacaaa gggcaagctg tcaagaaatt
      781 acagttattt tagtaaaaaa agaagcagaa aaatttcatt ttctactcct tttctatagg
      841 atttattttt ctttttcaga acttttaact tcagcttttt tggcagattt aatctgaatg
      901 tttcccttgc tagtcataac tgctttgaga tctttttcgc ttggattttc aaggtagtag
      961 tcagtgattg tgtcctcaat atagtcttga atagtccgac gaagtgggcg tgctcccatt
     1021 tttggatcat aacctaggtc aaccaacttt tccttgacct tgtcagttac atccaaacga
     1081 atgttgttgc tagagaggcg cttgttaaca tctgctagca tgagctcgac aatctgaagg
     1141 aggttatcct tgctgagagc cttaaattcg ataatgccat caaaacggtt cataaactct
     1201 gggctaaaga agttaccgag ttcaccgagg acagaattgg tacgtccttc tctagcagca
     1261 ccaaatccaa cgctagcttc ggtctttcct gtacctgcat ttgaggtcat gataatgatg
     1321 gcatccttga agctaacggt gcgtccttgc ccgtctgtca aacgaccatc gtccaagact
     1381 tgaagaaaca tgtgcataac atctgggtga gctttttcca cttcatcgag aaggatgaga
     1441 gaatacggat tgtggcgaac tttttcagtt aattgaccag cctcatcata gccaacataa
     1501 cctggaggag cgccgaccaa cttagccaca ctatgttttt ccatgtattc actcatatca
     1561 aagcgaatca tactatcagc agaaccaaaa agttcgatag ccagttgttt ggaaagttct
     1621 gtcttaccga caccagttgg tccaacgaag aggaagcttc cgattgggcg gttaggggta
     1681 ccaagtccga cacgattacg gcgaatagcc ttggcaatct tatcgactgc atcatcttgg
     1741 ccaataacat gagacttgag atcttcggct agatggatga gttgagattg ttctttctct
     1801 ttcaaatcac caacagggat attggttttc tgctcgataa tgtgctcaat agttttctcg
     1861 ctgatgatag gagtatcctg gtctgtgatc tttttctttt gcatttcctt atacttggca
     1921 atctggtcgc ggaagtaggc cgccttctca aaatcttctt ctcgtgtagc ttgagacttg
     1981 agattttcag cctcaatcaa gcgctgatca attactttag gatccacaaa attcaaggtc
     2041 aagttcatct tagaaccagc ttcatctagg aggtcaatgg ccttgtcagg caagaagcga
     2101 tcttggatgt agcgattgga aagagttgca gctgcttcaa tcgcagcatc ggtatattga
     2161 acgtggtggt aatcttcgta tttcttttga atccctttga gaatagtgat tgtttcatcc
     2221 accgttggtt catcgacttt aacaggctgc atacgacgct cgagggcagc atccttttca
     2281 atgatacggt attcattgag ggtagtagca ccgactagtt gcagttctcc acgagcaagg
     2341 gctggcttga ggatatttcc tgcgtccata ttaccatcac tcgcagaacc agcaccaaca
     2401 atttcatgga tttcatcgat aaagaggatg atgtcttcac gtttgcgaat ttcttccatg
     2461 agtttttgca tgcgttcttc aaattgtcct cgaatccccg ttccttgaac taagctaacc
     2521 acatccagac ggatgacttg tttaccttgg agtttatgtg gcacatcgcc atcgacaatt
     2581 ttctgagcta gaccttcgac aacggccgtt tttccgacac caggttcacc gataaggaca
     2641 ggattattct tggttctacg attgagaatc tcgatgacac ggataatctc atcgtcgcgc
     2701 ccaataacgg ggtcaatgtc tccacgacgg gcaatttcag ttacattaat accaaattct
     2761 tccagcaggc ctttttcttg gctaggtggc ggagtttgag cagatccacg attttgggaa
     2821 ccataaccgc cgtttccacc gtaacctcca cctgattggg ttgggggaat aggaggagta
     2881 ttgctagaag gtctgaaatt gtttagatca ttgaagaaat caccaaaggg atcgaagtca
     2941 cgattgttca gatccgtcat acctttgaag aggctattgt taggatctgt tttgataatc
     3001 ttatagcagt tttgacagag gtcaatttgt ttttgttttc cattgagatt ggtgtaaaga
     3061 tgaattgttg agtcgttaat tttacagttt tgacaaagca tacatctacc tcatttcttt
     3121 ctttagcctg acttgtttaa aggtcaaaaa tagtcaaatt ctatactctc attataacag
     3181 gattggggtg taaagcaagt aaaaagattg aagctttgag aagttgttac aatgaaaatt
     3241 tttgtgaatt tggactataa aaaaatccta tttgtgagac aaaaaggatt ttacttagac
     3301 atgcagggac aatcccaact tgttttagat tagtagagaa gttcgtaaat ttccattgcg
     3361 attaagtcaa tgctatcaaa cgtatacgtt tcgcgatctt ctacgtcacg aagggtaaag
     3421 attgggccgt tttcttcgtc gtggctgtac gcaacttctg caacaacaac tccttcgcgt
     3481 tcaaaattac gttttaaatt tccgccatcg tttgccattg cttcaaggcg gttgatgatt
     3541 ctaaccaaat gtgattccat tttgattctc cttcatttct tatcgttact attttaccat
     3601 aaaggagggc aacttgacta gaaaaaacta agatttctcg ctaattctgc tagtttaaag
     3661 atttttaaat aaattggata aaaaatttaa attttaattc aaaacatgtt ataatcatac
     3721 agtattctat tttagaaagc agtgtgacta tgaatttttc ttttttacct aagtatttac
     3781 cttattttaa ctatggggct gttgtgacga ttcttatttc tatctgtgtt atctttttgg
     3841 gaactatttt gggtgttgtc ttggcttttg ggcaacgttc aaagtttaaa ccgcttgttt
     3901 ggttggccaa cttgtacgtt tggattttcc gtgggacacc gatgatggtt caaattatga
     3961 ttgcctttgc tcttatgcat atcaatgctc cgactattca gattggaatt ttaggtgttg
     4021 atttttcgcg tctgattcca gggattttga ttatctctat gaatagtggt gcttatgttt
     4081 cggagactgt tcgtgccgga atcaatgcgg ttccaaaagg tcagctagag gcggcttatt
     4141 cgctagggat tcgtcttaaa aatgcgatgc gttatgtgat tttgccacaa gcagtcaaaa
     4201 atatcttgcc agcattgggg aacgaattta tcaccattat caaggacagc tccctcttat
     4261 cagctattgg ggtcatggag ttgtggaatg gggctacaac agtttctaca acaacctatc
     4321 tacctttaac accactttta tttgcagcat tttactactt gattatgacc tctattctga
     4381 cagtagcctt gaaagctttt gaaaaacata tgggacaagg agataagaaa taatgacaga
     4441 aaccttgata aaaattgaaa atttacataa atcttttgga aagaatgaag tattgaaggg
     4501 catcaacctc gagattaaaa gaggagaagt tgtcgttatc atcggtcctt cagggagtgg
     4561 gaaatctacc ttgcttcgct ctatgaattt gttggaggaa gcaaccaagg ggaagcttat
     4621 ctttgaggga gtcgatatta cggacaagaa gaatgacctg tttgccatgc gtgagaagat
     4681 gggcatggtt ttccaacaat ttaacctctt tcctaatatg actgtgatgg aaaatattac
     4741 cttgtcacct atcaagacta aaggtgaaag cagggaagtt gctgagaaga gagcccaaga
     4801 gcttttggaa aaagttggtt tgccagataa ggcagacgct tatccacaga gtctgtcggg
     4861 tgggcagcaa caacggattg ccatcgcgcg tgggttggct atggaaccag atgttttgct
     4921 ctttgacgaa ccaacttcag ccttggatcc tgaaatggta ggtgaggtat tggctgttat
     4981 gcaagacctt gccaagtcag ggatgactat ggttatcgta acacatgaga tgggatttgc
     5041 ccgtgaggtg gcagatcgtg ttatctttat ggcagacggt gtggttgttg aagatgggac
     5101 acctgagcag atttttgaac aaacccaaga acaacggact aaagatttct tgagtaaggt
     5161 tttataatct attttaaaat ttcaagacaa gattagtgaa aagttcaacc agagcttttt
     5221 cttatagttt gaaactatag gataacctag gaaagaagtg ttagagggca ctagcagttt
     5281 ttggtataat ggaagatatt tgaaagaaaa gagaagtgat atgacacaga ttattgatgg
     5341 gaaagcttta gcggccaaat tgcaggggca gttggctgaa aagactgcaa aattaaagga
     5401 agaaacagct ctagtgcctg gtttggtagt gattttggtt ggggataatc cagccagcca
     5461 agtctacgtt cgcaacaagg agaggtcagc tcttgcggct ggttcccgta gcgaagtagt
     5521 gcgagttcca gagaccatta ctcaagagga attgttagac ctgattgcta aatacaatca
     5581 ggatccagct tggcatggga ttttggttca gttgccatta ccaaaacaca ttgatgaaga
     5641 ggcggttcta ttggctattg acccagaaaa ggatgtggat ggtttccatc ctctaaatat
     5701 gggacgcctt tggtctggac atccagtcat gattccatca acacctgcag gaattatgga
     5761 aatgtttcat gaatatggga ttgaattaga aggtaaaaat gcggttgtca tcggtcgttc
     5821 taatatcgtt ggaaaaccca tggctcagct tcttttggcc aagaatgcaa cagtgacttt
     5881 gacccactca cgcacccata atcttgctaa ggtggctgct aaagcagata ttctggtggt
     5941 cgcaatcggt cgtgccaagt ttgtgactgc tgactttgtc aaaccaggtg cggtagtcat
     6001 tgacgttggg atgaatcgtg atgaaaatgg taagctctgt ggggatgtgg attatgaggc
     6061 agttgcccca cttgctagct atattacgcc agtccctgga ggtgtcggcc ctatgaccat
     6121 tactatgctg atggagcaaa cttatcaagc agcacttcgg acattggata gaaaataaga
     6181 gaaaaatcgc tgaagagagt gtattttcta tagctatatt taaaatggca gaaatgaata
     6241 ttaaatttta gatataagat tacaaaagga ggtctgcgcc tcctttttgt tgtataatgg
     6301 agtgaggtga ttagatgatt ttaaaaattt ataataggga atatagttta caatgggatg
     6361 gaatatacta cctagcacta attgattatc caaatattca agagtgggaa ttagaaaaaa
     6421 ttgctaaatt tatagcttac gaaaaacttc ataaacgtca aacaagtatt gagtgtgctg
     6481 attcttgttt aaaaaaagaa attttagatt acatctgtca gcatcccttt ctgccaccat
     6541 ttacccctac agataaaaga gtagcctcga cttatgatct acataagagg ttagtgactt
     6601 cagactactg tagtcatact acgactatag atgcagcgat ttctatcttt aaaacaggtc
     6661 agctcttatc tgctgtgaaa gcctttaggc gagatgctga agagttggtt ttggatagta
     6721 gaaatgctgc atcggatccg atagattatt ttgattatgt catgttaggg tggtcaaata
     6781 caagttctgg ttatcgatta gcgatggagc gtttattagg tcgagctcct tcagagaaag
     6841 aattacaaga caagtttatt cctggagtga gttttcattt tatctataca gatttgatta
     6901 aagttactgg ttatattttt gatggctacc atgttgcaaa aattaaggac atgttgaatt
     6961 tatttagtga gttgtatatt tgtattattc caactcataa taagagccaa tttgaaaata
     7021 ttattccaac taaaatacaa gatagggtgc attatcttga ctatgctgga gaagacttag
     7081 aagagtggac taagaaagtc tatcaagttg ttttaaaaca atcagataaa ggatagttga
     7141 ggaagaacga tgaaagtgat tgatcaagcc ttactagaaa aagttattat tgaacgttct
     7201 cgtactagtc ataaaggaga ctatggccgt ctgctgtttc ttggtggaac ttatccttat
     7261 ggtggtgcca tcatcatggc tgctttggca gctgtaaaaa gcggtgcagg attggtgacc
     7321 gttggaacag atagggaaaa tattccagcc ctgcacagcc atttacctga ggctatgtcc
     7381 ttttctcttc aagaccagca gttgttaaaa gagcaattgg agaaggcaga agttgtcttg
     7441 ctggggccgg gtttacgaga cgatgctttt ggagaagatc tagttaaaca ggtctttgct
     7501 ggcttaagac aaaatcagat tttgattgta gacggaggtg ccttgaccat tcttgctagg
     7561 acaagtttgt cattttcctc aagccagctt atcctaactc ctcaccaaaa agaatgggaa
     7621 aaattatctg gaattacgat tgaaaagcaa aaggaagatg caacggctag tgccctaact
     7681 tccttccctc aaggaacaat tttggtggag aaaggttcag ctactcgtat ttggcaagct
     7741 ggccaatcag attattacca gttacaggtt ggcggtccct atcaggcgac tgggggaatg
     7801 ggagatacgc ttgctgggat gattgcagga tttgcaggcc agtttaagca agctagtctt
     7861 tacgaacgtg tggcagtagc gacccatctt cattcagcca tagcccaaga actagctcaa
     7921 gaacagtatg tggttttgcc aacggagatt agtaattgtc ttcctaaagt aatgaaaaga
     7981 tatgtctaaa atagttagac aaaaaatatt gataatttgt atcattattc ttaattcaca
     8041 aaaaacgaac gtttagtatt cttctcgcta agaaactaaa tttgttcgtt tttttactct
     8101 tgtaaatcta tttttgttag agttgatttg gtttacatcc gtacttaaag tgatttgtca
     8161 gagttctgct tttattaaaa aaatttaatt tcaaggataa ataagcagta ttctaaaggt
     8221 acttttagat gaaataaaag cctttacatg gtataataga ggtagctctt taatggaggt
     8281 gtttgagtgg aaaatctgaa gaaaatggca ggtatcaagg ctgctgagtt tgtcaaggat
     8341 ggaatggtcg ttggacttgg aacaggctcg actgcctatt attttgtcga agaaatcggt
     8401 cgtcgtatca aggaagaagg attgcagatt atagctgtaa cgacttctag tgtgaccact
     8461 aaacaggcag aaggccttaa tatcccactc aaatctattg accaagtaga ctttgtcgat
     8521 gtgacagttg atggcgcaga tgaagtggat agtcagttta atggaatcaa aggtggtggt
     8581 ggtgctcttc tgatggagaa ggttgttgcg acaccatcaa aagaatacat ttgggtggtg
     8641 gatgaaagca agctggtcga aaaactaggt gcttttaaat tgccagtaga agtggttcag
     8701 tatggtgcag aacaggtctt tcgtcgtttt gaacgagctg gctacaaacc aagtttccgt
     8761 gaaaaagacg gccaacgttt tgtgacggat atgcagaatt ttatcattga cctcgccttg
     8821 gatgtcattg aaaatccaat tgcttttgga caagaattgg accatgtcgt tggtgttgtg
     8881 gagcatggtt tattcaacca aatggtggat aaggtcatcg ttgctggacg ggatggagtt
     8941 cagatttcaa cttcaaaaaa aggaaaatag aagggggcat aagatgtcta aatttaatcg
     9001 tattcatttg gtggtactgg attctgtagg aatcggtgca gcaccagatg ctaataactt
     9061 tgtcaatgca ggggttccag atggagcttc tgacacactg ggacacattt caaaaacagt
     9121 tggtttgaat gtcccaaaca tggctaaaat aggtcttgga aatattcctc gtgaaactcc
     9181 tcttaagact gtagcagctg aaagcaatcc aactggatat gcaacaaaat tagaggaagt
     9241 atctcttggt aaggatacta tgactggaca ctgggaaatc atgggactca acattactga
     9301 gcctttcgat actttctgga acggattccc agaagaaatc ctgacaaaaa tcgaagaatt
     9361 ctcaggacgc aaggttattc gtgaagccaa caaaccttat tcaggaacgg ctgttatcga
     9421 tgattttgga ccacgtcaga tggaaactgg agagttgatt atctatactt cagctgaccc
     9481 tgttttgcag attgctgccc acgaagacat tattcctttg gatgaattgt accgtatctg
     9541 tgaatacgct cgttcgatta cccttgagcg tcctgccctt cttggtcgca tcattgctcg
     9601 cccttatgta ggtgaaccag gtaacttcac tcgtacggca aaccgtcgtg acttggctgt
     9661 atctccattt tccccaactg ttttggataa attgaatgag gctggtatcg atacttatgc
     9721 tgtgggtaaa atcaacgata tctttaacgg tgctggtatc aaccatgaca tgggtcacaa
     9781 caagtcaaat agtcatggaa ttgatacact attgaagact atgggacttg ctgagtttga
     9841 aaaaggattc tcattcacaa acctagttga ctttgatgcc ctttacggcc atcgtcgtaa
     9901 tgctcacggt taccgtgatt gcttgcatga gtttgatgaa cgcttacctg aaattatcgc
     9961 agctatgaga gagaatgacc ttctcttgat tactgcggac catggaaatg acccaacgta
    10021 tgcaggaacg gatcacactc gggaatatat tccattgttg gcctatagcc ctgcctttaa
    10081 aggaaatggt ctcattccag taggacattt tgcagatatt tcagcgactg ttgccgataa
    10141 ctttggtgtg gaaactgcta tgattgggga aagtttctta gataaattgg tataagatga
    10201 cgcgctatgc tttgctggtg agaggtatca atgttggtgg taagaataag gtcgtcatgg
    10261 cggagcttcg tcaagaattg acaaacttgg gactggaaaa ggttgagagc tacatcaaca
    10321 gtggcaatat tttctttact tcgatagatt ccaaagccca attggttgaa aagctagaga
    10381 ctttctttgc agtccattat ccatttattc agagcttttc tttactgagt ctagaggact
    10441 ttgaggcgga acttgaaaat ctaccagctt ggtggagcag agatttggca cgaaaagatt
    10501 ttctctttta cactgagggt ttggatgtgg accaagtcat cgcgacagtt gaaagtttag
    10561 agctgaaaga tgaagtgctt tattttggaa aacttgggat tttctagggg aaattttctg
    10621 aagaatccta ttctaagact gcctatcata agtacttgct gaggtgcctt tctaccgcca
    10681 cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa aaaaataata
    10741 aaggagacac acaatgacat ttttaaacaa aatccatgaa actgctactt tcctgaaaga
    10801 aaagggaatt gcagcccctg agttcggtct aatccttggg tcaggacttg gagaattggc
    10861 agaagaaatc gaaaatccag ttgtagtaga ctatgctgag attccaaact ggggccgttc
    10921 aacagtagtc ggtcatgctg gtaaattggt atatggtgaa ctggcaggtc gcaaggtctt
    10981 ggctcttcaa gggcgtttcc atttctatga agggaatcct ctggaagtgg tgactttccc
    11041 agttcgtgtg atgaaagttc ttggatgtga aggtgttatt gtaaccaatg cagctggcgg
    11101 tatcggattt ggtcctggta ccttgatggc tatctcagac catatcaaca tgacggggca
    11161 aaatccattg atgggtgaaa acttggatga ctttggccca cgtttcccag atatgtctag
    11221 ggcctacaca ccagaatacc gtgctactgc ccatgaagtg gctaaaaaac ttaatatcaa
    11281 gcttgatgaa ggtgtctata tcggagttac tggtccgact tatgaaacac cagcagaaat
    11341 tcgttcctat aagacactgg gagcagatgc agttggtatg tctacggttc ctgaagttat
    11401 cgtggcagcc cactctggct tgaaagttct gggaatttca tgtatcacta actttgcggc
    11461 tggtttccaa gaagaactca atcacgaaga agttgtagaa gtgactgaac gtgttaaagg
    11521 tgattttaaa ggcttgctta aagcgattct tgctgaattg taagaaaaaa gatttaaaag
    11581 ggggagtgcc tctgtctttt caggattgac tgcctatccg tattaaagaa gaaacagagg
    11641 aatactatga gcttcttcct gctcttataa ctgaaagaag cggaagaata ggtgtgtctg
    11701 atctgatagc tagcattgtg aaagacaaga ttctaggata ctagcattag cttcctagcc
    11761 aagcagacta gtatgataag gagagatgag aatgaattga ctttctgaat ttctcagtct
    11821 tatcatatat agcacaatga gatttcgctt gagtctgctt gtaaataaac gaaaagaaag
    11881 ataagaaata atgaaaattg gtcaacgaat tatgcgcttt ggcataaaaa attaagtatc
    11941 ggagttgtat ctgttgtagt cggctttgat ttctagctcc agctggaatt tcagccaatg
    12001 aagtaaagca agatgtaaca actgaagtgg taataggtgt gctagattct aaggaggaat
    12061 tgaaagagtc agaaaatgat gctccaaaac tagaaactcc tcttagagag gagccaagac
    12121 tagctcctca aacgcttccg gaagcaagtg aagttcttga aaacaaaagg gaagagtcaa
    12181 aagtagagat aacagagcca gctcaagcgg atgacatccg caaggttgtt ggggaattag
    12241 ccaaggatat aagtattact aagttgtata tgacaggtca ttctcttgga ggctacctag
    12301 ctcagattgc agcggttgaa gattaccaaa aatatcctga tttttataac catgtattga
    12361 ggaaagtgac aactttcagt gctcctaaag tgattacttc cagaactgtt tgggatgcta
    12421 agaatggttt ctgagatgtt ggtttggaaa gtcgtaaatt agctgttagt ggaaaaatta
    12481 agcattatgt ggttgataat gacaatgttg tgactccctt gattcataat aatcgtgata
    12541 ttgttacatt tacaggtaat tcacgcttta aacaccgttc tcgtggctat tttgaaagtc
    12601 caatgaatga tattcctaac tttaatattg gtaaacaagc taccttggat aaacatggtt
    12661 atcgtgatcc gaaattggat aaagtgcgat tctttaagaa acaggctcta cctcaatctt
    12721 ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt aggaaaacag gttactcaaa
    12781 gttcgacagc tttcggagga gatgctagaa gagctgtgga tggcaaagtc gatggtaact
    12841 atggtcacaa ttctgtcact catacaaact tccaatctaa gccttggtgg caagtagatt
    12901 tggctaaaga agaaaccatt cgccaaatca atatttacaa ccgaacagac actgcccagg
    12961 atagattggc aaactttgat gtcattcttt tagacagttc tggtaaagaa attgagtgaa
    13021 aacgtataat atctcctaaa gatgtgtcag cacaaattac gattaaccat aaaaaagcgc
    13081 gctatgttcg gattgggcta gaaggctata atgccctcag tcttgcagaa gttgaagttt
    13141 tccgctttat agctacgaat gctgaaacgg cgacacaagt ttctaagcca gttcaaccaa
    13201 tcagtcagac tcctgtgaaa gataaaacat tgacaattca acacagtgga gcttacattg
    13261 cccgctactc cataacttgg gaagaagttc cagtagataa agatggaaac caagttgttc
    13321 gtagtcattc ttgggaagga aacggttgca accagactgc aggttttgtc ctcaacttcc
    13381 caatcaaaga aaatatgaga aatctgcgag ttaagattga gaaaaagacg ggcctactat
    13441 ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt agctcaaccc caccgtaaaa
    13501 ttacccattg gggtacgaca ttgaattcca aggtgagtga cgatgatgtc ttgtaatctg
    13561 atggtacaat gacagttagt ttgtctagtt tataagaaag tactatctga gcttgaatag
    13621 aactcaggta gctctctgtg aaagaacaaa attaatactc aatgaaaatc aaagagcaaa
    13681 ctaggaaact agccgcaggt tgctcaaagc actgctttga ggttgtagat aagactgacg
    13741 aagtcagtca catatataat ccaaggcgac gttgacgtgg tttgaagaga ttttcgaaga
    13801 gtataaacag aaaggtagag cgcgtgttct aatttgaaca cgagtagaaa acttttctaa
    13861 aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac tgaatacggg cgactctcct
    13921 ctaaatcaaa attaagaaag aaaggaattg accccaccct aaaagcagtg ggaaaaagat
    13981 agttggtcta gcgagcatcg ctcactgcgc ccaactccta ttttcccttc gctttttgac
    14041 gggtttggta tctttctcaa tataaaatat aaaataaaga aaggtagagc gtgtgttttg
    14101 atttgaacac gagcggaaaa ctcggaaaat agataatctg actgaaaaat caggatttct
    14161 cgtcggcttc ctaattttca gtcgttttct tctcgctctt tgtatcataa attatgtcta
    14221 tccatattgc tgctcagcag ggtgaaattg ctgataaaat tcttcttcct ggggatcctc
    14281 ttcgtgctaa gtttattgcg gagaatttcc ttgatgatgc tgtttgtttt aacgaagtgc
    14341 gtaacatgtt tggttacact ggtacttaca agggtcactg tgtatctgtc atgggaactg
    14401 ggatgggaat gccatctatt tcgatttatg cgcgtgagtt aatcgtagac tacggtgtga
    14461 agaaattgat tcgtgtggga actgcaggtt ctttgaatga agaggttcat gttcgtgaat
    14521 tagttttggc gcaggcggct gcaaccaact caaacatcgt tcgtaatgac tggccacagt
    14581 acgattttcc acaaattgct agctttgatt tgcttgataa agcctaccat atcgccaaaa
    14641 aacttggtat gactactcac gttgggaacg ttttgtcatc tgatgtcttt tactcaaatt
    14701 actttgaaaa gaatatcgag cttggtaaat ggggagtcaa ggctgtggaa atggaagcag
    14761 cagctcttta ctatcttgct gcccaatacc atgttgatgc gctagctatc atgaccatct
    14821 ctgatagctt ggtcaatcca gacgaagata caactgcaga agaacgtcaa aataccttca
    14881 ctgatatgat gaaggttggt ttggaaacct tgattgcaga ataattatag ccaaaaagga
    14941 agccctttat gaattgtagg acttcctttt cttatccaga aattgatcta gttctctctg
    15001 atttcgaaga atagtgactt tatgtgaata ttcttggcaa agtttttggt aattttcttt
    15061 ttgagttttg ctacgcccat cccaaagaat ccatctgata aactcccact caaagcgttc
    15121 agggcaatct gccgccatac tttctctgac ttttccacgg tatttaagat aacgcttaaa
    15181 ggctctaaag agacaggtca atggcaaaaa attgagaaag atgatttggt cagcttcttg
    15241 cattcgttct tggtagtagc accaagaata attaccatcg atgacccaag ctttatgctt
    15301 ggtgagaaag ttttttatct cggttaacat ccattcgcag tcactgtctt gccaaccagg
    15361 ttgaaattgg agtgtgtcca tgtgcagttt tggaatggag tagtagttag ataacttttc
    15421 tgctagagtt gacttaccag aaccagaata tccgataatt gcgattttca ttttctacct
    15481 tttcctattt ggagacaaaa aaacagcctc tatggactgt ttcttattta gcaagtttag
    15541 ctgaaagacg agctttatcg cggcttgctt tgtttttgtg aatcaaacct ttagtttctg
    15601 ctttatcgat agctgagcta gcagcacgga aaagttcttc agatgggttt gcttcgaaag
    15661 cttttatagc agtacgcata gctgattttt gagctgagtt cttttcgttt tgtttaacgt
    15721 tcaattcagc gcgtttgata gctgatttaa tgtttgccaa tgttcttacc tccatattta
    15781 ctaactatac cattatatat gaaaacttac gttttgacaa gggaaaatta cttttttaag
    15841 taaatttcat cgatttcatg gttcttgctt ttatgaagaa tcacttctgc acgatttctg
    15901 gttggttcaa tataattttg cagatttgtg agattgatac tggtccagac ctgatgggca
    15961 aaggactcca cttccccaat cggcatctga gtaaaacgat aatagtagct atcagggtcg
    16021 ttttgggcta ggctcagcat tttcaagaaa cggtccagat accaactttc aatatcatcc
    16081 actccagcat ctacatagat ggaaaagtca aagaagtcag tgatatagag acgatcgttt
    16141 tgtggatttt gaaagacatt aattccctca acaattacaa aatcagcagc tttgacactt
    16201 tgtttctttt cgggtacgat gtcgtaaact tcatgagaat agacaggaat atctacatct
    16261 tgtccatttt tgatgcggtc caagaagttg agaagagctt ccatatcata gctttcagga
    16321 aatcctttac gatttaaaat cccctgctca atcaaggttt gattgggata gagaaaacca
    16381 tcagttgtaa ccaactcaac cgtagcatct gtaaacgtac gggacagtag gatttgaagt
    16441 aggcgactgg ttgtggattt tccaacggca acactcccag aaaccccaat aataaaaggt
    16501 tgagatttac tttcacgttg gaggaaaatt ccttttgaaa aggctaaatc ttccttagtt
    16561 cgcttgtaaa tctgaatcag atgagccaag gggagataga tatctgtaac gtcttggaga
    16621 ctgatttggt cattaaaact cttgatagat tccaattctt cttctgtcaa aggaggtgtt
    16681 gtctttcgat gtaaagattg ccaagtctgg cggctgattt tttcaaaatg taaaaattcg
    16741 ttggtcattt gttttcccct agatattttg tctatcatac cataaaaagc taaaaaaata
    16801 aagcggtttc ttgaatgtgg taagtcaatg gtatatagta taagtgatca atttattgat
    16861 gaaataggag atagattcta ctgatttata agcttataaa gggggctttt tagttcacaa
    16921 cttataggag gtgtactatg aaaatcttaa aacgttacat attggaactc tgttttattt
    16981 taagttttgc tttacctttt acaaaaggag ccaatgcaga taatggtaga tgctttgtgg
    17041 aaacctatta cggttttact tttttgatgg aacatgctat tgtaacagct gtctttatct
    17101 gttcgatctt aattgctttc ttactaaaaa aacgatgggc gaaatggatt gctgctggta
    17161 gttattgctt tttagtccta tggattgcga cagagggtta tttcttccgc atgtcactag
    17221 aagatttgat acgactttgg acaagtttgg aaatcctgac acaaacgtat cagttgggct
    17281 tttatctaaa cattttgttg ggaattttgt tgataataaa gtattttaag gttaagcaat
    17341 agaaataata atctgcctga ttatagatga taaacttttt gtttttatct ggactgtgat
    17401 atttatagtt ctaaatggtt tattatttat aagtgttgaa agagattgaa gaaaatggct
    17461 tcgtgactcg ctttcttcgg tctttttcgt tttattatca atttgtaaac gatttacaat
    17521 ttgagtcaaa ttatggtaaa atagtgtcat gagtaaaatg tattatgcag aaaatcctga
    17581 cgctgctcac gacattcatg agttgagagt agagttgttg ggacaaaaaa tgaccttttt
    17641 aacggatgcg ggtgttttta gcaagaaaat agttgacttt ggaagtcaac tcttgctcaa
    17701 gtgtcttgag gtcaaccaag gagaaactgt ccttgatgta ggctgtggtt acggaccttt
    17761 gggtttgtcc ttagctaagg cttatggagt tcaggctact atggttgata tcaacactcg
    17821 tgccttggat ttagctcgga gaaatgctga aaaaaataat gcaaaagcga cgattttcca
    17881 gtccaacatc tatgaacaag ttgagggaca ttttgatcat gtcatttcca atcctcctat
    17941 ccgtgcgggc aagcaagtcg ttcatgaaat cattgagaaa agtaaagatt tcttggaaac
    18001 tggtggggat ttgacaatcg tcatccagaa aaaacaaggg gctccaagtg ctaagtccaa
    18061 gatggaggat gtgtttggta attgtgaaat ccttaaaaaa gacaaaggat actatatcct
    18121 tagaagtgtg aaagaatgag agcagttgat ttaatacaaa aaaaacgaga cggtcaagaa
    18181 ctgacttcaa gtgaaattga atggctgata gaaggctatg tgtcaggaac tgttcctgat
    18241 tatcagatgt ctgcctttgc tatggctgtt tattttaaag gaatgacgac tcgtgaaatt
    18301 tctgatttaa cgatgaatat ggttaagact gggcaagagt ttgacctgtc agccattgat
    18361 ggtgtgaaag ttgacaagca ttctactggt ggtgtcggcg ataaggtaac cttgatttta
    18421 gctcctcttg ttgctagctt cggtgtgcct gtagcaaaaa tgagtggtcg tggtcttggc
    18481 catacaggcg gaacaattga taaattggag tccattaagg gctatcaagt agaacgtagt
    18541 caagaggatt ttattcgtca ggttcaggat attggagtat ctgtcattgg gcaatcagac
    18601 cagctggtca aagcagataa gcttctctat gcccttcgtg atgtgaccgc aactgtcgac
    18661 acgattcctt tgattgcgag ttcggtgatg agcaagaaaa ttgcggcagg ggcggatgct
    18721 attttgctag acgtgactgt cggtgagggt gccttcatga agacggttga tgaggcgcgt
    18781 gagttggctc aaactatggt agatcttggt aaggttgttg gacggaagac agtagcagtg
    18841 attacggata tgagccagcc tttgggacga gcgattggaa atcgtctgga aatccttgaa
    18901 gcattggaga ttttgcaagg tcaaggacgt caggatatta cccactttat ttgtgaattg
    18961 gctcaaatta tgcttggttt ggcaaatgtt aacaaaactg ttgaagaagt tcgccaacat
    19021 cttgagaatg gtcaagcact ggctaagttt gaggaaatgg tgcaagccca aggtggagac
    19081 ttggaagacc tctattgtcc tgtaaatgta gctcatgtgg tggaaatccc tgctcaggaa
    19141 acgggtgtca tttcagctct tccagctatg gactttggtc tttatgccat gaggctgggt
    19201 gctggtcgtg cagttaagtc tgatgccttg gactatgaaa caggaattgt ttttgaaaag
    19261 aagattggag actccgttca aaagggtgaa attgttgcaa aagtatacac aaatgaaaaa
    19321 attccacctc aactagttac agatttccaa aaatgtgtta aaataagtga tgaagtgaaa
    19381 aaaatccgtg aaattgtaga aatcatctcc taaatggagg gaaaaccgaa atgaaattaa
    19441 ataaatatat agatcatacg cttttaaaac aagatgcaaa gaaaaaacaa attgatagtt
    19501 tgttgtctga ggctagagag tatgactttg ccagtgtttg cgttaatccg acctgggttg
    19561 aacatgctaa aaaaggactt gaaggcacag atgttaaggt ttgcacagta gtaggtttcc
    19621 ctttgggagc aacaacttca gccgtgaaag catttgagac aaaagaagct atccaaaatg
    19681 gtgcagatga gattgatatg gtgatcaatg ttggagctct caaatcaggt aatttagcct
    19741 tggttgagtc agatattcgc gcagtagtgg aagcaagtgg tgataagtta gtgaaagtca
    19801 ttattgaagc ttgccttctg acagaccaag aaaaaattgt tgtttgccaa ttggcccaaa
    19861 aagctggggc tgactttgtt aaaacatcta ctggtttttc aactggtggt gctacgatag
    19921 cagatgttac attaatgcgt gaaacagttg gatctgatat gggagtcaag gctgctggtg
    19981 gagctcgttc ttatgcagat gctcttgcct ttgtcgaagc aggtgcgacc cgtatcggaa
    20041 cgtcagctgg ggtagctatt ttaaaaggag aattggcaga tggcgactac tgagttgatt
    20101 gaactggcaa ttgaaaccag caaacatgcc tatgtcccct attctcactt tcctatcgga
    20161 gcagttttag tagccaaaga cggaagtgtt tacacgggag tgaatatcga aaatgctagc
    20221 tatcctttga ccaattgcgg tgagagaaca gctgttttta aggctatatc tgaaggccaa
    20281 agagaatttt cagaattgat tgtctatggt caaactgaaa aaccgatctc gccatgtggt
    20341 gcttgtcgcc aagtaatggt cgaatttttt gaacaagatt taaaagtgac cttagtcgca
    20401 aaagataaat cgacggtcga gatgacggtc ggggaattac ttccatactc ttttacagac
    20461 ttaaactagt ctgagtcgct ctctgagtgg cacggtcctt gtggccaacc aatccatact
    20521 tgcaacatcg ttgcacatct tatttaggag gttcagtaat gaacaagaaa caatggctag
    20581 gtcttggcct agttgcagtg gcagcagttg gacttgctgc atgtggtaac cgctcttctc
    20641 gtaacgcagc ttcatcttct gatgtgaaga caaaagcagc aatcgtcact gatactggtg
    20701 gtgttgatga caaatcattc aaccaatcag cttgggaagg tttgcaggct tggggtaaag
    20761 aacacaatct ttcaaaagat aacggtttca cttacttcca atcaacaagt gaagctgact
    20821 acgctaacaa cttgcaacaa gcggctggaa gttacaacct aatcttcggt gttggttttg
    20881 cccttcacaa tgcagttgaa gaagcagcta aagaacacac tgacttgaac tatgtcttga
    20941 ttgatgatgt gattaaagac caaaagaatg ttgcgagcgt aactttcgct gataatgagt
    21001 caggttacct tgcaggtgtg gctgcagcaa aaacaactaa gacaaaacaa gttggttttg
    21061 taggtggtat cgaatctgaa gttatctctc gttttgaagc aggattcaag gctggtgttg
    21121 cgtcagtaga cccatctatc aaagtccaag ttgactacgc tggttcattt ggtgatgcgg
    21181 ctaaaggtaa aacaattgca gccgcacaat acgcagccgg tgcagatatt gtttaccaag
    21241 tagctggtgg tacaggtgca ggtgtctttg cagaggcaaa atctctcaac gaaagccgtc
    21301 ctgaaaatga aaaagtttgg gttatcggtg ttgatcgtga ccaagaagca gaaggtaaat
    21361 acacttctaa agatggcaaa gaatcaaact ttgttcttgt atctactttg aaacaagttg
    21421 gtacaactgt aaaagatatt tctaacaagg cagaaaaagg agaattccct ggcggtcaag
    21481 tgatcgttta ctcattgaag gataaagggg ttgacttggc agtaacaaac ctttcagaag
    21541 aaggtaaaaa agctgtcgaa gatgcaaaag ctaaaatcct tgatggaagc gtaaaagttc
    21601 ctgaaaaata atggatggaa gtcatttctt aggaagcggc caagggccgc ttttttaaga
    21661 gttgagacaa agtcattttg tctcagtaaa gcttgctact agacaaacta gcaagtttta
    21721 ttgaaataaa ataagactct gaaaggaaga gcacatggca cacgaaaatg tcattgagat
    21781 gcgtgatatt accaaggtgt ttggtggatt tgttgccaac gacaaaatca acttgcacct
    21841 acgaaaaggt gaaattcatg cacttttagg agaaaatggg gctggtaagt ccacgctaat
    21901 gaacatgtta gcaggccttc ttgaaccaac tagtggtgaa atcgcggtca acggtcaagt
    21961 tgtcaatctc gactccccat ctaaagcagc tagcttggga atcgggatgg ttcaccagca
    22021 ctttatgttg gttgaagcct tcacagtggc tgaaaacatc attttaggta gtgaattgac
    22081 taaaaatggt gtgctagata tcgctggagc tagcaaagaa atcaaggctc tttctgaacg
    22141 ttatggctta gctgttgacc cttctgccaa ggtagcagat atctcagttg gagcccaaca
    22201 acgtgtagaa attttaaaaa cactttatag gggggctgat atccttatct ttgacgaacc
    22261 aacggctgtt ttgactccat cagaaattga tgagttgatg gctattatga aaaatcttgt
    22321 caaagaagga aaatcaatta tcttgattac ccacaaattg gatgaaattc gcgcagtttc
    22381 tgaccgtgtt acagttatcc gtcgtgggaa atcaattgaa accgttgaaa ttgcaggggc
    22441 taccaatgct gatttggcgg aaatgatggt aggacgttct gtttccttta aaacagagaa
    22501 gcaagcctct aaaccaaaag aagtggtttt gtctatcaaa gatttggtgg tcaatgaaaa
    22561 ccgtggtgtt ccagctgtta aaaatctgtc cttggatgtt cgtgctggag agattgttgg
    22621 tattgcgggg attgatggaa atggtcagtc tgaactgatt caagccatta caggtcttcg
    22681 taaggttgaa tctggtagca ttgagctaaa aggagattca attgtaggct tgcacccacg
    22741 tcagattaca gaactaagtg ttgggcacgt tccagaagac cgtcaccgtg atggcttgat
    22801 tttggaaatg atgatatctg aaaatattgc ccttcaaacc tactataaag aaccacatag
    22861 taaaaatgga attttgaatt attcaaatat tacttcttat gctaaaaagc tgatggaaga
    22921 gtttgatgtt cgcgctgcca gtgaattagt tcctgcagct gcactctcag gaggaaatca
    22981 acaaaaagca attattgctc gtgaaattga tcgagatcct gatctcctta tcgttagcca
    23041 gccaactcgt gggttggatg tcggtgccat tgagtatatc cacaaacgct tgattgaaga
    23101 gcgtgataat ggcaaggctg tccttgttgt cagctttgaa ttggatgaga ttttaaacgt
    23161 ctcagaccgt attgccgtta tccacgatgg taagattcaa ggtattgtat caccagaaac
    23221 aaccaataaa caagaacttg gtgtcttgat ggctggtgga aacttgggaa aggagaagag
    23281 tgatgtctaa aaaattacaa caaatttcgg ttcccttgat ttctgtattt ctaggaattt
    23341 tactcggagc cattgtcatg tggatcttcg gttatgatgc tatttggggc tacgaagaat
    23401 tgttctatac agcctttggc agtctgcgtg ggattggaga aatcttccgt gctatgggtc
    23461 ctctggtctt gattggtctt ggttttgcgg ttgccagtcg agctggtttc tttaacgtcg
    23521 gacttcctgg tcaggctttg gcaggttgga ttctcagtgg ttggtttgcg ctgccgcatc
    23581 cagatatgcc ccgtcccttg atgattctag caaccatcgt gattgccttg attgctggtg
    23641 ggattgtcgg agcgattcca ggtattctta gggcctatct agggacgtca gaggttattg
    23701 taaccatcat gatgaactac attgtcttgt atgtagggaa tgcctttatc catgctttcc
    23761 ctaaagaatt catgcaaagt acagattcga ccattcgtgt tggggctaat gcaacctatc
    23821 agacaccttg gttggctgag ttgactggta actcacggat gaatattggt attttctttg
    23881 ccatcattgc cgttgcagtt atttggttca tgctcaagaa aacaactctt ggttttgaaa
    23941 tccgtgcagt tggtcttaat ccacatgctt cagaatatgc tggtatttct gccaagcgga
    24001 ctattatcct atctatgatt atttcaggtg ccttggcagg tcttggtgga gctgttgaag
    24061 gtttgggaac cttccagaac gtctatgttc aaggttcgtc attagctatc ggatttaacg
    24121 gaatggcggt tagtttgctt gcggccaact caccaattgg tatactcttt gcagccttcc
    24181 tatttggcgt tctccaagtt ggggctcctg gtatgaatgc ggcgcaggta ccatctgagc
    24241 ttgtcagcat tgtaacagcg tctattatct tctttgtcag tgttcattac cttatcgaac
    24301 gctttgtcaa accgaaaaaa caagttaaag gaggtaagta aagatgtcta taataacctt
    24361 gctccccctc ttggtgtctt ctatgctgat ttactcagca cctctcatct ttacaagtat
    24421 cggtggtgtt ttctctgaac gtggtggtgt ggtaaacgtt ggccttgaag gaattatggt
    24481 tatgggtgcc ttttctggag ttgtctttaa ccttgaattt gcagaacaat ttggagcagc
    24541 aactccatgg ctatccttgc ttgtagcagg attggttggt agtgttttct ctatcatcca
    24601 cgcagcagcg acggttcatt tccgtgcaga ccatgttgtc agcggtacgg tattgaactt
    24661 gatggcgcct gccttggctg ttttcttagt taaagttctt tataacaaag gacaaaccga
    24721 caacctaagt caaacttttg gacgctttga tttcccagtc ttggcaaata tcccagtgat
    24781 tggtgatatc ttcttcaagt caactagtct acttggttat ctggcgattg ccttctcatt
    24841 ccttgcttgg tttattctct ttaagactca atttggtctt cgtctccgct ctgtcggtga
    24901 acatcctcaa gcagcggata ccttgggaat caacgtctac aagatgagat atttagggat
    24961 tattatttca ggttttctag gtggaattgg cggagcgatt tatgcgcaat caatctcagt
    25021 taacttctca gtgacaacta ttgttggacc tggatttatc gcccttgctg cgatgatctt
    25081 tgggaaatgg aatccaatcg gtgctatgct ttctagtctc ttctttggac tttcacaaag
    25141 tttggctgtt atcggttctc aattgccgtt cctacaagga gtgccagcgg tttatcttca
    25201 aattgcacct tatgttttga caattcttgt cttagcagcc ttctttggaa aagcagtcgc
    25261 accaaaagca gatggtatca actacatcaa atcaaaataa gcatacaaaa aaacgtcagt
    25321 ccaatgactg aactgccccc caaaagttag acagaaaaaa tctaactttt ggggtgtttt
    25381 tattatgaaa ttgagttgtg atgataaagt tcagatctat gaacttagaa aacaaggata
    25441 tagcttagag aagctttcaa ataaatttgg gataaacaat tctaatctta ggtatatgat
    25501 taaattgatt gatcgttacg gaatagagtt cgtcaaaaaa gg
//
LOCUS       DAYYQN010000028        25119 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYYQN010000028 DAYYQN010000000
VERSION     DAYYQN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.028"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(319..1161)
                     /locus_tag="ACHBRQ_001090"
     CDS             complement(319..1161)
                     /locus_tag="ACHBRQ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9592778.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(1174..1314)
                     /locus_tag="ACHBRQ_001091"
     CDS             complement(1174..1314)
                     /locus_tag="ACHBRQ_001091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592779.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEVSNNTYGKATLMWFEES
                     IK"
     gene            complement(1417..2808)
                     /gene="pgdA"
                     /locus_tag="ACHBRQ_001092"
     CDS             complement(1417..2808)
                     /gene="pgdA"
                     /locus_tag="ACHBRQ_001092"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HFM9592780.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKMEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(3046..3414)
                     /locus_tag="ACHBRQ_001093"
                     /pseudo
     CDS             complement(3046..3414)
                     /locus_tag="ACHBRQ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            3653..4630
                     /locus_tag="ACHBRQ_001094"
     CDS             3653..4630
                     /locus_tag="ACHBRQ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9592781.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFRKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            4627..5709
                     /locus_tag="ACHBRQ_001095"
     CDS             4627..5709
                     /locus_tag="ACHBRQ_001095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9592782.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     SEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(5773..6652)
                     /locus_tag="ACHBRQ_001096"
                     /pseudo
     CDS             complement(5773..6652)
                     /locus_tag="ACHBRQ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612110.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            6966..7271
                     /locus_tag="ACHBRQ_001097"
                     /pseudo
     CDS             6966..7271
                     /locus_tag="ACHBRQ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            7403..8347
                     /gene="manA"
                     /locus_tag="ACHBRQ_001098"
     CDS             7403..8347
                     /gene="manA"
                     /locus_tag="ACHBRQ_001098"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9592783.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(8369..9844)
                     /locus_tag="ACHBRQ_001099"
     CDS             complement(8369..9844)
                     /locus_tag="ACHBRQ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFM9592784.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            9927..10681
                     /locus_tag="ACHBRQ_001100"
                     /pseudo
     CDS             9927..10681
                     /locus_tag="ACHBRQ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(10701..11156)
                     /locus_tag="ACHBRQ_001101"
     CDS             complement(10701..11156)
                     /locus_tag="ACHBRQ_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HFM9592785.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(11176..12351)
                     /locus_tag="ACHBRQ_001102"
     CDS             complement(11176..12351)
                     /locus_tag="ACHBRQ_001102"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9592786.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(12410..13255)
                     /locus_tag="ACHBRQ_001103"
     CDS             complement(12410..13255)
                     /locus_tag="ACHBRQ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9592787.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            13380..13937
                     /locus_tag="ACHBRQ_001104"
     CDS             13380..13937
                     /locus_tag="ACHBRQ_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9592788.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            13956..14423
                     /locus_tag="ACHBRQ_001105"
     CDS             13956..14423
                     /locus_tag="ACHBRQ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFM9592789.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            14512..15141
                     /gene="upp"
                     /locus_tag="ACHBRQ_001106"
     CDS             14512..15141
                     /gene="upp"
                     /locus_tag="ACHBRQ_001106"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9592790.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            15315..15905
                     /gene="clpP"
                     /locus_tag="ACHBRQ_001107"
     CDS             15315..15905
                     /gene="clpP"
                     /locus_tag="ACHBRQ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HFM9592791.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            15984..16232
                     /locus_tag="ACHBRQ_001108"
     CDS             15984..16232
                     /locus_tag="ACHBRQ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9592792.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            16333..17493
                     /locus_tag="ACHBRQ_001109"
     CDS             16333..17493
                     /locus_tag="ACHBRQ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9592793.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            17761..18630
                     /locus_tag="ACHBRQ_001110"
     CDS             17761..18630
                     /locus_tag="ACHBRQ_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HFM9592794.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            18634..19590
                     /locus_tag="ACHBRQ_001111"
     CDS             18634..19590
                     /locus_tag="ACHBRQ_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HFM9592795.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            19590..20354
                     /locus_tag="ACHBRQ_001112"
     CDS             19590..20354
                     /locus_tag="ACHBRQ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592796.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            20354..21064
                     /locus_tag="ACHBRQ_001113"
     CDS             20354..21064
                     /locus_tag="ACHBRQ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592797.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            21478..22134
                     /locus_tag="ACHBRQ_001114"
     CDS             21478..22134
                     /locus_tag="ACHBRQ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9592798.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            22242..23337
                     /gene="prfB"
                     /locus_tag="ACHBRQ_001115"
     CDS             join(22242..22313,22315..23337)
                     /gene="prfB"
                     /locus_tag="ACHBRQ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9592799.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            23355..24047
                     /gene="ftsE"
                     /locus_tag="ACHBRQ_001116"
     CDS             23355..24047
                     /gene="ftsE"
                     /locus_tag="ACHBRQ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9592800.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            24040..24966
                     /gene="ftsX"
                     /locus_tag="ACHBRQ_001117"
     CDS             24040..24966
                     /gene="ftsX"
                     /locus_tag="ACHBRQ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9592801.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVETNTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
BASE COUNT         7640 a         4939 c         5155 g         7385 t
ORIGIN      
        1 atttatattt tgtatcaaat gatatttgag aaatttcttt caatacaatt acctccttga
       61 taaattctat atagtataaa attcaaaaag aaccaccttt caacacccaa gccttgcgac
      121 taggggcgtc agaaacgcgg ttccacccta atttattact tcattgtttt tgaaaatact
      181 acagaaagcg ccagctcatt attttgccct acttggctcc cactgtccca agctcgcttg
      241 aagaacgcca taagactttc tttcctgctg actattatat caaaaattag aataatgtac
      301 aattcttttt atgattttct agaaatccac atcatcaact cgtggagcac ccgattgaac
      361 agcaatcgtt tttaaggttt ctctctcctc atgactcagt tcaattccaa agcaatcaag
      421 attagcttga atacgagagg ttgtgacaga ttttggaagt ggtaaaaatc cttctgccaa
      481 gctccaggcc aaggctatct gagcaacaga ctttccgtga tttgctgcta tttcttggac
      541 ttgcttgcta tcaaacagtt ctccttgtcc aaaaggcccc caagcttcca ataaaattcc
      601 cttttcacga cagtaagcta cgacttcctc ttgatacaca cctggcgcca agcgaacttg
      661 attgaccgca ggaacgatag ttgcagtttc aagcaaggca tccaaatgat ggggaagaaa
      721 attgctaacg ccgatagcac ggattttccc ttcttgatag aggtcttcca tcgctctcca
      781 aacttccgca ttgcgagttt tccatgcgtc attttctctg agcggttttg ggttcggcca
      841 atgaatcaaa tacaaatcca aataatccaa gcccagtttt tctatagatt tttccaaagc
      901 ttgacgagtt tgctcatagg tttgctgact attccaaagc ttggtagtta cgaacatttc
      961 ttcacgtgga actccgctat ctttgattgc ttgaccaaca ctttcttcat tctgataaat
     1021 cgccgccgta tcaatatgac gataaccagc cttcaaggct tctaacactg cacgataggc
     1081 ttcttctcca tccttagcct taaaagttcc aaatcccaat actggaattt ctactccatt
     1141 atttaattga taggtattca tatgcttctc cttctatttt atactctctt caaaccacat
     1201 caacgtcgcc ttgccgtagg tattgttact gacttcgtca gttctatcca caacctcaaa
     1261 acactgtttt gagcaacctg cagctagttt cctagtttgc tctttgattt tcattgagta
     1321 ttattcatca attatagcat aaaacatttt ttaagtagta ctatcttttg taaaaaataa
     1381 gtctatcaca tatctgacag acctattttt tcttgcttat tcatcacgac tataatacag
     1441 ctcatgagct tttaggcgag tattgagcat ctctggtatg gtcacaaagg tataaccttg
     1501 atttttcaaa tactcaatga cccttggcaa ggcattgact gtcggactgt gaatatcatg
     1561 catcaaaacg atagagccgt tagctacttg atgctgaatt tccgtcaaaa tagctgcttc
     1621 atttttactc ttccagtcca gactatccac atcccacata ataaagctca aatcgaggct
     1681 attgcgaata tcatctgtaa tagctccata aggcggacgc atgagtttgg aactagaacc
     1741 cagcacttta gttagcacat cctcagtatc agtaatctgc tttttagctt catcaagaga
     1801 tagttgcgag agaataggat ggctccaact atggtttcca acaacatgac cttcagattt
     1861 tatcctcttc accaagtcct cattcccaga aacatttttc ccaagcacaa agaaagtcgc
     1921 tttaatatca tatttagcta gggtctctaa tacctgcggg gtcgttgctg gatttggacc
     1981 atcatcaaag gttagagcga caactttttg atgtttctta tcaaagtaag attggtacaa
     2041 ggccgcatct ttttcgagta agtacgaaga ttggataaca tcaaagaaag cagatactgg
     2101 caaggctatc tcttctaaat tttcaaccac aggacttgga taaaggataa tctgactatc
     2161 cttgtaatca aaattccatg cagacaagtc ttggtcagag aagtttttta caatctgctc
     2221 actctggtct tgctccattt ttttatcctc tatgaaggag gtcaactctt ttatcagctg
     2281 ttccttagcc ttactagcat ctgaaaacag ttggtcaagt gtaaaaggtt gcccatcttc
     2341 tgttaagtga acctttccta gactggtctt ttcagtctct tcaatcttaa aagcaactaa
     2401 atcatagatt tgtttggtca cattacgatt aacgactccc tttaaacctg actcttcttg
     2461 ctctgtatag tagaaaacaa gattgtcctt actttctagc ttgtccttaa catcttgatt
     2521 tatcagctcc ctaacagagg aaatcacttt ctccccttgg agaggataat aggcaatcac
     2581 ttcggcttgc ccctgacgaa aatgctcctt ctgatttccc tcactcaatt gatcatcttt
     2641 ctcttttttg agcgattcaa tcttttgctc aaaacttttt tgctggtaga tcttaaaagc
     2701 aataaatccg cctaataggc aaatagaaat tgctaaaata ccaatcaaag ccaataatac
     2761 atgtctcgtt ttcccgtgtc tgccacgtcc tagtctactt ttattcatat ttatatcata
     2821 acaaatttga gcaataattt caatgataca atttggcaat ccacaattcc tctagcaagc
     2881 caaagcatct tacatcaact ccaacatccg tatctgtaga gattatgtag ttttctatct
     2941 atggaatttc tagtagattg aagctagaat agtagaccta tacttctaaa acattgttag
     3001 aaatcgattt gactgtcctg atcgatttgt cctgttctca tttcatttta ctataaaact
     3061 atgaaaagct aggtcatctg agaagtaatt actatcgaca attgcgatag cgtatactag
     3121 tgcgatttgt ttgtagctct ggtgggtatc accttcacgt tggcgaattt tttctaggtt
     3181 ttgattaacc taactgcaca gataagccca taggcgattg atgaaaaaat tctgatgatg
     3241 aacctgaatt tcgatgataa cttggattcc attatctaac tccgccaaga cgtctatact
     3301 agtatagaaa tcctgcgctg agtagggcat ggaaggcaag acgtgaatgt tacttccctc
     3361 caaaatcgtc acattttttg ctggcaagtc cagcatatcg cggataaatt gacaaatcat
     3421 tagttgggct gatacccgga tgtctgagaa tcctcctttt cctccttcta ttatagcaca
     3481 tttttaaatc taggtttgct agattctatg cttctatcta tttattcgga aaagaaagct
     3541 tttcttcaga agaaagctaa tagcttattt gaaattttct cctctctgtt ggatagtccc
     3601 gtctatctat gttataatga aagaaggatt taaaatcgga aaaggagtat ttatgctaaa
     3661 attaggtgtc atcggaactg gcgctatcag ccatcatttc atagaagcag cccataccag
     3721 tggagaatac caactggtcg caatctattc tagaaaacta gaaactgcag caacctttgc
     3781 ttctcgctat cagaatatcc aactattcga tcaattagaa gtcttcttca aatcttcttt
     3841 tgatttagtc tatatcgcca gtccaaactc cttacatttt gctcaggcaa aagctgcctt
     3901 gtctgctggt aaacatgtta ttctcgaaaa gccagctgtc actcaaccac aagaatggtt
     3961 tgatttgatt caaacagctg aaaaaaataa ctgttttatc tttgaagcag ctcgtaatta
     4021 ccacgagaaa gcctttacta ctatcaaaaa ctttttagca gataagcaag tgttaggagc
     4081 agatttcaac tatgccaaat actcttccaa gatgcctgac ttgttgactg gacaaacacc
     4141 aaatgtcttt tcagaccgtt ttgctggtgg agccctcatg gatttgggga tttatcccct
     4201 ctatgctgct gttcgtcttt ttagaaaagc caatgacgcg acttaccatg ctcaacagct
     4261 tgacaatagc attgacctaa atggagatgg tatcctcttc tacccagact atcaagttca
     4321 catcaaggct ggaaaaaaca tcacttccaa tcttccttgc gagatttata caacagatgg
     4381 aaccttgact ctcaacacga ttgagcatat tcgctcagct atttttaccg accaccaagg
     4441 aaatcaagtc cagcttccta tccaacaggc tcctcatacg atgactgagg aagtcgctgc
     4501 atttgcacac atgatccagc aaccagatct gaatctctac caaacttggc tgtatgatgc
     4561 aggatctgtt catgagctac tatataccat gcgccagact gctggcatta gatttgaggc
     4621 agaaaaatga aaaccaaact accgactgaa tggcaagaac tgagtgacca actcggtttc
     4681 caagaattca cccccattca aactcaacta tttgagcccc ttcttgctgg agaaaacctc
     4741 ctaggagtga gccaaaccgg aactggtaag accctagcgt atctcctacc aagtcttctc
     4801 agactacaaa agaaaaaagc ccaacaactc ttgattctag caccaaatac ggaacttgct
     4861 ggacagattt ttgatgtatg taaaacgtgg gcagaagcta tcggattgac tgctcaactc
     4921 ttcctttcag gttcaagtca gaaacgacaa attgaacgcc ttaaaaaggg gccagaaatt
     4981 ctgattggaa ctcctggccg tatctttgaa ctcattaaac ttaaaaaaat caagatgatg
     5041 aatgtggaaa ccatcatcct ggatgaattt gaccaattac tagatgattc tcagattcac
     5101 tttgtagaaa aaatcactca ctacgcacct cgtgaccacc aactggtcta catgagtgcc
     5161 acgaccaagt ttgaccaaga aaagattgtg ccgaatacac gcactattga tctctctgac
     5221 caaaaattgg acaatatcca gcatttctac atgcaggtag accaacgtca tcgagtggat
     5281 atgctacgaa aactggctca tgtagaggat ttccgtggtc tagtcttctt caatagcctg
     5341 tcagaccttg ggaatgccga ggaaaaacta cagtatcgtg atatattgac tgtttccctc
     5401 gctagtgatg ttaatgttaa atttagaaaa atcatcttag aaaagtttaa agataaccaa
     5461 ctaaccctgc ttcttgcaac tgaccttttg gctcgtggaa ttgatatcga tagcctagaa
     5521 tgtgtcgtaa actttgatat tcctagagat agcgaaacct acacacaccg tgctggccgt
     5581 actggtcgta tgggcaagga aggatatgtt atcactctcg tcactcattc agaagaaatc
     5641 aaaaaactta aaaagtttgc aagtatccga gaaattgtcc taaaaaacca agaactctat
     5701 atcaaataaa gccccattgc tctatacaca tccagtgtat ggagcttttt tataagcaat
     5761 tcaattttat actcatctgc tttcaaaaag cattctagtc catctccgat taacgatggg
     5821 ctttatcacc tccttctcca gtccttgtat gatatcttga agttgattca tgacatcttc
     5881 caaagtttga aaggctttat tcttaaatcc acgtttacga atctctttca acacttgttc
     5941 aatggggttc atctctggtg tgtatggagg aataaaggta aaaccaatat tagtcggaat
     6001 ctttaaggta cttgatttat gccatatagc attgtccata acgaataaaa gataatcatc
     6061 tggataagct tgtgaaagct cttctaaaaa gacgttcatc cactcagtat tacatccacc
     6121 agctattaag aaaaatgatt cgcctgtatg ggcatcaaca gctccataac aatagcgaaa
     6181 ttctcgtata tagtgactat ggacatgtgg acctactcct attggagacc aacaagatcc
     6241 cagtttactg attctaccga aaccagcctc atcttgggac atcaagtgaa ccttacgaaa
     6301 acgtctactg gttttaaatc gctttctttt cttcttgaat tgagatttta tttttagacg
     6361 cgacaatggt ttgagcgtct gctttcttag gatgttctgg acgtggcgta atatttcgcc
     6421 aaccatggcg cttcaacaat tgatagaagg catcacgtgt gtaggaacga cctaactcct
     6481 ttttataagc ctgaaataag gcatcaattg taacaaattc tcctgcctct gcagccttca
     6541 aatggcgggc aagaaagact ttcttttcct caactgtcat atatgcatgg ttacgaccac
     6601 cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc atattttttt acattttgct
     6661 aaatcgttgt ttgattacag tctaaaagct ctataatctc tttataagat ttgcccatca
     6721 gacgaaatat aataaattga aactagaata gtacacctct acttctaaaa cattgttaga
     6781 aatcgatttg actgtcctga tcgatttgcc ctgttcttgt ttcgttttac tatagaacga
     6841 tttgaaggcg tttatgatat ttagctgtac gagagtcttt aaaaagtgtt ttgatggttt
     6901 ggatttcttc tttagttgat ttcatatcac tattatataa tgctttttga ttttagtata
     6961 gtattatcgt agaaaatgga ctcggtgcta tggatacacc ggatgagaat ggttatgtag
     7021 cagatgacta tcggattact tacttagagg cccacatcaa ggccatgcga gatgccattt
     7081 acaaagacgg ggttgacttg cttggttata cgacatgggg ctgtatcgat tcggtttcag
     7141 ctggcacagg ggaaatgaat aagcgctatg gctttatcta tgtagatcga gataatgtag
     7201 gaaatgggac tctcaaacgt agcaagaaga agtccttcta ttggtacatg tcatttatag
     7261 caatggtgta agcatcgagt agagcacatt tgtttactat ttttataaaa tcttctccct
     7321 gctttataag atgtaaaaaa gagttcaatt cgaactcttt ttttgctata atgaggggag
     7381 aaaaatcaga caggagatta agatgtcaga accattattt ttacaatcag ttatgcaaga
     7441 aaaaatctgg ggtggtacca agctacgtga tgagtttggc tacgacatcc caagtgaaaa
     7501 agtcggagag tactgggcta tctcagctca tctaaatggt gtttctaaag ttgccaatgg
     7561 tcgttacgag ggaacagatc ttgctacttt gtatgcggaa caccgtgaat tatttggcaa
     7621 tcgtcctgag cctgtatttc cgcttttgac taagattttg gatgctaacg actggctcag
     7681 cgttcaagtt cacccagacg atgcttatgg actagagcat gaaggcgaac ttggaaaaac
     7741 agaatgctgg tacatcatcg ctgcggatga aggttcagag attatctacg gccacaatgc
     7801 caagtcaaaa gaagaactcc gccagcaaat cgaggataag aactgggatg cgctgttgac
     7861 aaaagtgcca gttaaagcag gagatttctt ctatgtacca agcggtacca tgcacgctat
     7921 cggggcgggt atcttgattc ttgaaaccca gcagtctagc gataccactt accgtgtcta
     7981 tgactttgac cgtaaggatg acaagggcaa tttacgtgaa cttcaccttg aaaaatctat
     8041 cgatgtcttg aacattggtg aacccgcaaa tagccgtcca gtgacaatca aagcagatga
     8101 tctacgttcc actctccttg tatccaatga tttcttcgca gtttacaaat gggaaattac
     8161 tggaaaagtt aactttgaaa agacagctga ctacagcttg ttgagtgtct tggctggtca
     8221 aggtcaacta actgttgacg ggaaaaacta tccaatccaa aaagggagac actttatcct
     8281 accaagtgat gttgaagctt ggactctgga agggcaagat ttagaattaa ttgttagtca
     8341 tccataaaaa agaaagggcc tgagttcatt actcaagtcc tttttgatta cataaattgg
     8401 gcgatgaaga ccacgatgat gattggaatg acggaacgaa gaaacgaaga aggaagagcc
     8461 agacttggaa cagtccttgt ttccatgctc tttcatcgag atggagttcc tccattgcaa
     8521 gagccttttt aaagatatag cctgtaaaaa gtgaaaggta gagagctcca aatggcatga
     8581 ggagattggg aaccaagaag ttcatagcgt caaagaaggt cttaccaaaa atgtgaacat
     8641 ccgccatgac accgtaagat agggctgaag gaatgccaaa gacaaaggtc aaaattccta
     8701 aaataacact ccatttggca cgtttgctgt tatcctggtt ggtgatattg cccacattga
     8761 tctccagcat cacgacagaa gaagtgacag tcgcaaagag gaagaggacg tagaaaatgg
     8821 ttccaaaagg catcttgtca aagagttgag gcaagacgat aaagagcaag ctcggtcccc
     8881 cttcagactg gatattgaag ggggaccgag cttggaaaat ggctagacct gccatgatgg
     8941 atatcgagat attcatggct acgatggaga ttcctgactg gactagattg gttttcttgt
     9001 ccaagtaaga agcataggtc aacatgactg taacccctag tgagagggca aagaaagatt
     9061 gtcccagagc atagaggaga ccagcgctag tcagttttga aaagtctggt ttgaggaagt
     9121 aaagaacccc ttccatggca tttggcaaac tgagagagcg accgatgata acaacaaaga
     9181 tgataaagag caggggcatc atgactttcg aagctctttc aatccctttt tgaaccccac
     9241 gtgatacaat aaagatattt aataagataa aggctgcttg agctcctagg gcaatggctg
     9301 gatttgaaat gattgaagta aataactgag cataatcacc cgttccacca agttggaaca
     9361 atttcccaaa ctcaatacct agatagacta gaatccatcc tccgataaca ctgtaaaaag
     9421 ataagaggat aaagagggca aaggcgccaa tccaaccgat aaagttgtac ttgttattct
     9481 tgcccagttt tccaaaggtt ttgatagcgg aaacgccagc actacggcca agggcaaact
     9541 cagccagcag gagagggaaa ccgattaaaa tagtggaaat gagaaagatt agtaaaaagc
     9601 ctccaccgcc attagcagca gtcatgtagg gaaacttcca aacggaacca agcccgatga
     9661 ccgagccagc agatgctaga ataaagccaa gtttcgaccc ccattgtgat ttttctgaca
     9721 tagttaaact ctgatctcct ttttcaaaat aagaaaaagc tacttgagtt gtcaaatagc
     9781 cttctgtttg attgatagac ttgactatcc tattatgttt tctaaggtct gtcaagaaaa
     9841 ccattttcaa gttttcacac ctttctcaaa aacgcttgac tctgacctaa ggggaggggt
     9901 tatactatca ttgtaaggag gaaatcatgt accatataaa agaagctgcg cagctttcag
     9961 gtgtctctgt caagaccctg ccctgcatca ctatgacaag ataggactct tggtcccctt
    10021 aaagtcggaa aacggctatc gaacctatag tcaagaggat ttggaacgcc ttcaggtcat
    10081 tctttactac aaatatctag gcttttcttt agagaaaata gcagaactgt taaaggaaga
    10141 aaggacagat ttattgctcc atttgactag gtagttggac tatctaactc gcgaaaggca
    10201 acatctggat accttgattt ccaccttgca aaaaactatt caagaacaaa aaggagaaag
    10261 aaaaatgacc attgaggaaa aattcacggg atttagctat caaaacaatc aaaaatacca
    10321 ccaagaagcg gtagagaaat atggtcaaga agtcatggga caagcgctcg aacgccaaaa
    10381 aggtcacgaa gacgaggcta cggccgcctt taaccaagtc tttcaaactt tggcacaaaa
    10441 tcttcaagtt ggtttacctg caacagcaac cgaaaaccag gagcaagcag ccaagctctt
    10501 gcaagccatt cgcacttatg gatttgactg ctctattgag gtattcggtc atatcggtaa
    10561 aggttacgtc tacaacccag agtttaagga aaacattgac aagtttggtt ctgaaacagc
    10621 ccagtacacg tcagatgcca ttgcggctta cgttcagaca aatgcagaaa aaataggcta
    10681 ggaatttcct agcctatttt ttacttcaaa tcataaagcc agtcgtcacc gtttttgtag
    10741 taaaagaatt cactgagatc ttcttctaga aacacacgaa gcatatcaga catatcatcg
    10801 gttgcaagtt ttagatgaga aagattttca aagtcctccc accaaacttt cccttcgtct
    10861 gaagactgga gttcaccagt aaagtgttct gtcttgtaaa aaaggacgac ataacgataa
    10921 tccttgtcgt cataccagtt tttgatacca cagagttggg gtttggaaat gatcagacca
    10981 gtttcttctt tcacttcacg aatgacagca tcgacaaagg attcgccacg ttcaacatga
    11041 ccaccaggaa aagtaatgcc agaccagtcg ggactaactc ggtcttggac caggacctta
    11101 tctccgtttt taatcataca catgttaaca aattcgactg cctctcttct gttcattctt
    11161 cacaaccttt aatctttaat cataatgcag acttcccgcc acccagccgg tacagagggc
    11221 agaagtgatg ttaaagccac ccgtgtgggc attgatatcc ataacttcgc ctgcaaagtg
    11281 gaggccaggt accagcttac tttcaagggt tttaggattg atttccttga gactgactcc
    11341 acccttggta acaaaggact ttgcaaggga catttttcca gttacaggaa ttttaagttc
    11401 tttaatggac tggacaagtt gttctcgttc cttttcagtc agttgtttga ctttttcagg
    11461 atatccttgt acaaaaaatt cggccaagcg ttctggtaac aaggttttta aagcgttttt
    11521 caaggatttt tcccgatttt cttctagaaa tgtaaccaag tccttctcag aaagttgagg
    11581 caaaacatcg agtgagagaa cctccccacc tttgacaaag ctagacatgc gtagggcagc
    11641 aggacctgac aaaccaaagt gggtaaagag taaatcatga gtgatgacat gcttaccata
    11701 acttagggtc acatcgtcca gagaaatccc ttgtaaggct ttatgtggaa aatctgttaa
    11761 taaaggactt tcagcagcct caagatcggt gatggtatgc ttaaaatggc gagcaatctc
    11821 gtgaccaaaa ccagtcgaac cagtcgaagg ataagactta ccacctgttg tgacaatgag
    11881 tttctcacaa gtgaaggttt gatccgctga cttaaggaca aactggtcat ctactttttt
    11941 aacagaaacg atttctattt gagtagcaac ttgaccacct agttcggtga ttttcttttc
    12001 caaagcttcg ataatagtcc gagacttgtc actggctgga aagacgcgtc cgtggtcttc
    12061 gaccttaagt ttaacaccat tttctgtaaa aaagttgatg atgtcatgat tatcgaactg
    12121 ggagaaaaca ctgtaaagaa agcgtccgtt tccaggaatt ccagctagca ggttgtctaa
    12181 gctaccattg ttggtcacat tgcaacgtcc cccaccagtc ccagctaatt tttttccaag
    12241 tttccgattt ttttcgatga ggagggtttt ctgtccataa aagctactgg aaatcgtagc
    12301 catcatacca gcaggtcccc caccgatgac aatagtatca aaatgtttca tagctctatt
    12361 gtaccacaaa aaaacaagag atgatggtca cctcttgtca agaatgcaat taatcaattt
    12421 catagcccat cagcaaaccg ccctcttctg catagaaact gcagagacca gaggttggta
    12481 gaattttaat atccgcttgt gggaaggttt cacggattcg ctctgagagc tgttgacaac
    12541 atttttcgtt attgcgttgg gccatgacaa tacggccacc agcatatcca gcttttacta
    12601 actcatcata ggcagcttga actgatttct ttgatcccct tgctttttgt agcaattcga
    12661 gagttccagt ttcactagct tttccgacca tacgaatgtt gagaaggcca acgaccgtac
    12721 cgataagctt gctcaaacgg ccgttcttca ccaagttatc gactttggct aggacaaaga
    12781 gcaacttagt tttttcttga taggcagtga tagcttcaac cacttcttca aaagacaagc
    12841 cctggtcaat caagtcattc aatttttcta cgagtaggtc aacttcacca ccagcagata
    12901 aactatcaat cacatgaatc ttagtgtcag gatggtcttc tagataaata ttctttgcta
    12961 gttgagcact attgtgactg ccagaaaggg tacctgtgat ggttactagg aaaatgtttt
    13021 tggcaccttc aaatgctcgc aaatagtcat ctgggcttgg acaagccgat tttgaagctt
    13081 ctgcagttgc atacatggtt tccatcattt ggtcaatatc gagactggcg tcatcaacaa
    13141 agacctgatc agctacttga atggttaagg ggacacttac aaaggttgtg ttaatagctg
    13201 gtgttggcag ttgacgataa tcacaaccag agtcagcaat aattttccaa gtcatagaaa
    13261 ttctccatct ttgtcagtta tacattgaca aaggttctgt ctttttttac aattatatca
    13321 tgaaaaccct tgaaacaaaa gcatcatccg tctgttgtga caagtagaaa gaaaatgtta
    13381 tgtctgaacg tagaatctct gaaaagtctc ttgaaaatct cagaaaatca aaccaagaat
    13441 ccaatttatt aaccagagaa gccattgaaa cagccctctt gcaactcttg gaaaaaaagg
    13501 agctgaccaa gattagtatt tctgaattgg tcaaacgtgc aggtgtttcg cgtgcggcct
    13561 tttatcgcaa ttatgactcc aaagaggaaa ttttagagag cgtctttaaa cgaactgtcc
    13621 acaatattat ggaacagctg catcattacg atttaaagac agacctttat ttggtttggg
    13681 ttcacctttt ccgggaggcc agaaaggaag ctagagtgat tcaattggcc ttggattacc
    13741 atctggaaaa aatctttgtc caagccatgc aggaatttct agaaaaatac catgggaaat
    13801 caaaaggtgt cagctcttat cttcattcct tctggagctc agccatcgtc tctgtccttc
    13861 taaaatggat caaggatggc atgaaggtac cagctgaaaa gattgcagat ttaggtttac
    13921 cattttttaa aaaatagaga aaaaggagaa aagagatgac tgaaaaaaga ctggcctggg
    13981 atgagtattt tgcagcccaa gccctactaa ttgcgaatcg ttccacttgt aaacgtgcta
    14041 aagtgggcgc gattctggta aaagataata aggttatttc cactggttac aatggttcgg
    14101 tgtcagggac tgagcattgt attgatcacg aatgtttggt cattgaaggc cactgtgttc
    14161 gcacccttca cgctgaggtc aatgctatcc ttcaaggtgc tgagcgtggt gttcccaaag
    14221 gctttacagc ctatgtaacc cattttcctt gtctcaactg tacaaaacaa ttgcttcagg
    14281 tcggctgcaa gcgcgtggtt tatatcaacc agtaccgaat ggacgactac gcccaatacc
    14341 tttatcaaga aaaagggaca gaattgaccc atttaccact tgagacagta cagacagctc
    14401 ttaaagaggc tgatctaatg taaaaattat caaaaataaa tggcttagaa agatttttaa
    14461 accgtttttt ggtataataa gaagaataaa ttgaaagaag gaattccaaa aatgggaaaa
    14521 attgaagtta ttaatcaccc actgattcaa cacaaattgt caatcttgcg tcgtacagat
    14581 acttctacaa aagcttttcg tgagctagta gatgagattg caatgttgat ggggtatgaa
    14641 gtacttcgtg atcttccact agaagatgtg gaaatcgaaa caccaattac aaaaacagtt
    14701 caaaaacaat tggcaggtaa gaaattggcc atcgtcccaa tcttgcgtgc aggtatcggg
    14761 atggttgatg gtctcttgag cttggttcca gctgctaaag ttggccacat cggtatgtac
    14821 cgtgatgaag aaacacttca accagttgaa tacttggtga aattgcctga ggacattgac
    14881 caacgtcaaa tttttatagt agacccaatg ttggcaacag gtggctcagc aatcttggct
    14941 gttgattctc ttaaaaaacg tggcgcatca aatatcaaat ttgtctgcct tgtatctgct
    15001 ccagagggtg taaaagccct tcaagaagct catccagatg tagaaatctt tacagcagcc
    15061 ttggatgaac gtttgaacga acacggttat atcgttccag gtcttggaga tgctggagac
    15121 cgcttgttcg gtacaaaata agatcgaaaa gaagaatgac ttggaaaagt atttccagtc
    15181 acgaaaggag gttgggtttt tgtttctgtc taatgaaagc agagcaaaaa tttgaccttt
    15241 tttgaccaag atattataat agtcttatct tcagtcattt gaccaacaaa attaaaactc
    15301 aaaaggagaa atgaatgatt cctgtagtta ttgaacaaac aagccgtgga gaacgttctt
    15361 acgatattta ctcacgtctt ctcaaagacc gcatcattat gctgacaggt ccggttgaag
    15421 acaatatggc taactctgtt attgcccaat tgcttttctt ggatgcccaa gatagtacaa
    15481 aagatattta cctttatgtc aatacaccag gtggttctgt ttcagctggt ttggcaatcg
    15541 tagataccat gaactttatc aaggcagatg tccaaaccat tgttatggga atggctgcat
    15601 ctatgggaac agtcatcgca tcaagtggag caaaaggcaa acgtttcatg cttccaaatg
    15661 ctgaatacat gattcaccaa ccaatgggcg gtacaggtgg tggtacccaa caaactgata
    15721 tggctatcgc tgcagaacac ttgctcaaaa ctcgtaatac cttggaaaaa atcttggctg
    15781 aaaattcagg tcagtcaatg gaaaaagtcc atgcagatgc agaacgtgat aactggatga
    15841 gcgcccagga aacacttgaa tatggcttta ttgatgaaat tatggccaac aattcattga
    15901 actaatgatg atagaaggca aactcgactg ggtttgcttt ttttggtata atagggagag
    15961 atttcttaga aagaggattt atcatgtttg aaaaagtcaa tcgctctggc ttgattatct
    16021 atctttacta taatcgtgat gccaaaaaac tgcaggatta tggagatatt acctatcatt
    16081 ccaagaaaca tcgttactta caactctatg ttccaactca agaagtggag caattggtcg
    16141 gacgcttgag caaggaaaaa tttattaaaa aagttagggt ttgtcatatc caagagttgg
    16201 aaacaccctt tgtgggcaat ctttatcgat aggaaaacgt tatcatcgaa aaagttcaag
    16261 gaaaacattg acaattttct gataattcgg tatattctta acagactatt taagaaataa
    16321 ggagacaaaa agatgaagaa aaaatttgcc ctatcgtttg tggcgcttgc aagtgtagca
    16381 cttcttgcag cctgtggaga agtgaagtct ggagcagtca acactgctgg taactcagta
    16441 gaggaaaaga caattaaaat cgggtttaac tttgaagaat caggttcttt agctgcatac
    16501 ggaacagctg aacaaaaagg tgcccaattg gctgttgatg aaatcaatgc cgcaggtggt
    16561 atcgatggaa aacaaatcga agtagtcgat aaagataata agtctgaaac agctgaggct
    16621 gcttcagtta caactaacct tgtaacccaa tctaaagtat cagcagtcgt aggacctgca
    16681 acatctggtg cgactgcagc tgcggtagcg aacgctacaa aagcaggtgt tcccttgatc
    16741 tcaccaagtg cgactcaaga tggattgact aaaggtcaag attacctctt tattggaact
    16801 ttccaagata gcttccaagg aaaaattatc tcaaactatg tttctgaaaa attaaatgct
    16861 aagaaagttg ttctttacac tgacaatgcc agtgactatg ctaaagggat tgccaaatct
    16921 ttccgcgagt catacaaggg tgaaatcgtt gcagatgaaa ctttcgtagc aggtgacaca
    16981 gacttccaag cagcccttac aaaaatgaaa gggaaagact ttgatgctat cgttgttcct
    17041 ggttactata atgaggctgg taagattgta aaccaagcgc gtggtatggg aattgacaaa
    17101 ccaatcgttg gtggtgatgg attcaacggt gaggagtttg tacaacaagc aactgctgaa
    17161 aaagcatcaa acatctactt tatctcaggc ttctcaacta ctgtagaagt ttcagctaaa
    17221 gctaaagcct tccttgacgc ttaccgtgct aagtacaatg aagagccttc aacatttgca
    17281 gccttggctt atgattcagt tcaccttgta gcaaacgcag caaaaggtgc taaaaattca
    17341 ggtgaaatca aggataacct tgctaaaaca aaagattttg aaggtgtaac tggtcaaaca
    17401 agcttcgatg cagaccacaa cacagtcaaa actgcttaca tgatgaccat gaacaatggt
    17461 aaagttgaag cagcagaagt tgtaaaacca taatagaaaa atgttgaaat agggaatgag
    17521 cctttgactc actccctgtt tcgatattta atactcttcg aaaatctctt caaactgcgt
    17581 caacgtcgcc ttggattata tatgtgactg acttcgtcag tcttatctac aacctcaaag
    17641 cagtgctttg agcaacctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
    17701 aagaacctat caaaaagtga gggaaacccc tcggaattat aaatagaaag agtgaatctt
    17761 atgctccaac aactcgtaaa tggtttgatt ctaggtagtg tttacgcgct gttagcccta
    17821 ggatatacca tggtttacgg aattatcaag ctcatcaact tcgcccatgg tgatatttat
    17881 atgatgggag cctttatcgg ttatttcttg atcaattctt tccaaatgaa tttctttgta
    17941 gcgcttattg tagctatgct agcgacagct attcttggtg tcgtgattga gtttcttgct
    18001 taccgacctt tgcgccactc tactcgtatt gctgttttga ttacggctat tggggtttct
    18061 ttcctattgg agtatggaat ggtctatctg gttggtgcca atacccgtgc cttccctcaa
    18121 gcgattcaaa cagttcgata tgatttggga ccaattagct taacaaatgt gcagttaatg
    18181 attttggcta tttccttgat tttgatgatt ttgttacaag tcattgtcca aaagactaag
    18241 atggggaaag ccatgcgtgc agtatcagta gatagcgacg cggcgcaatt gatggggatc
    18301 aatgtaaacc gtacgattag ctttaccttc gctttgggtt ctgctcttgc gggtgcggct
    18361 ggtgttctga ttgctcttta ttataactct cttgagcctt tgatgggggt tactccaggt
    18421 cttaaatctt tcgttgccgc agtacttggt ggtatcggaa ttattcctgg tgcggctctt
    18481 ggtggctttg tgattggtct attggaaacc tttgcgactg cctttgggat gtcagatttc
    18541 cgtgatgcca ttgtttatgg aatcttgttg ttgatcttga ttgtccgccc agctggtatc
    18601 cttggtaaga atgtgaaaga gaaggtgtaa acgatgaagg aaaatttaaa agttaatatt
    18661 ctatggttac tccttttgtt agctggctat agcttgatta gtgtactggt ttcagtcgga
    18721 gtacttaatc tattctatgt acagatttta cagcaaattg gaattaatat tattttggct
    18781 gttggtctca acttaatcgt tggtttttca ggacaatttt cacttggtca tgctggtttc
    18841 atggcgattg gtgcctatgc agcagctatt attggttcta aattaccaac ctacggtgcc
    18901 ttctttggag ctatgcttgt aggggctttg ctttcaggag cagttgcctt acttgtcggc
    18961 attccaacct tgcgcttgaa gggggactat cttgcggtag caactctggg tgtttctgaa
    19021 attatccgta tctttatcat caatggtgga agccttacaa atggtgcggc aggtatctta
    19081 gggattccta actttacaac ttggcaaatg gtttacttct ttgtcgtgat tacaaccatt
    19141 gcaaccttga acttcttgcg tagcccaatt ggtcgttcaa ccctctctgt tcgtgaagat
    19201 gaaatcgctg ctgagtcagt tggggttaat acgactaaaa ttaaaatcat cgcttttgtc
    19261 tttggtgcca ttactgcaag tattgctggg tcacttcagg caggatttat cgggtctgtt
    19321 gtaccgaaag attacacctt catcaactca atcaacgttt tgattattgt tgtatttggt
    19381 ggactcggtt ccattacagg tgcgattgtt tcggctattg ttctgggaat tttgaatatg
    19441 cttctccaag atgttgctag tgtgcgtatg attatttacg ctttggcctt ggtattggta
    19501 atgattttca gaccaggtgg actccttgga acatgggaac tgagcctatc acgtttcttt
    19561 aaaaaatcta agaaggagga acaaaactaa tggcattact tgaagtaaaa cagttaacca
    19621 aacattttgg tggtctaaca gctgttggag atgtgactct tgaattgaac gaaggggaac
    19681 tggttggatt aatcggtcca aacggagctg ggaaaaccac ccttttcaac cttttgaccg
    19741 gtgtttatga accaagcgag ggaacagtaa ccctagatgg tcaccttttg aatgggaaat
    19801 caccttataa gattgcctct ttgggacttg gacgtacttt ccaaaatatc cgtctcttta
    19861 aagatttaac agttttagac aatgttttga ttgcttttgg aaaccatcac aaacaacatg
    19921 tttttactag tttcttacgc ttaccagctt tttacaagag tgaaaaagaa ttaaaggcta
    19981 aagctttgga attgttgaaa atctttgatt tagatggtga tgcagagact cttgctaaaa
    20041 atctttccta cggacaacaa cgtcgtttgg aaattgttcg tgcccttgct acggaaccta
    20101 aaattctctt cttagatgaa ccagcagcag gtatgaaccc acaggaaaca gccgaattga
    20161 ctgagttaat tcgtcgtatc aaagatgaat ttaagattac aatcatgttg attgaacacg
    20221 atatgaatct ggtcatggaa gtaacagaac gtatctacgt acttgaatat ggccgtttaa
    20281 ttgctcaagg aactccagac gaaattaaga ccaataaacg cgttatcgaa gcttatctag
    20341 gaggtgaagc ctaatgtcta tgttaaaagt tgaaaatctt tctgtgcatt acggtatgat
    20401 ccaagcagtc cgtgatgtaa gctttgaagt taatgaagga gaagttgttt cccttatcgg
    20461 tgccaacggt gcaggtaaga caactattct tcgcacattg tcaggtttgg ttcgaccaag
    20521 ttcaggaaag attgaatttt taggtcaaga aatccaaaaa atgccagctc agaaaattgt
    20581 ggcaggtggt ctttcacaag ttccagaagg acgccacgtc tttcctggct tgactgttat
    20641 ggaaaatctt gaaatgggag ctttcttaaa gaaaaatcgt gaagaaaatc aagctaactt
    20701 gaagaaggtt ttctcacgct ttcctcgtct tgaagaacgt aagaaccaag atgcagctac
    20761 tctttcagga ggggaacaac aaatgcttgc catgggacgc gctcttatgt caacaccaaa
    20821 acttcttctt ttagatgaac catcaatggg acttgcccca atcttcatcc aagagatttt
    20881 tgatatcatt caagatattc agaagcaagg aacaaccgtc ctcttgattg aacaaaatgc
    20941 caataaagca cttgcaatct ctgaccgagg atatgtactg gaaacaggga aaatcgtcct
    21001 atcaggaaca ggaaaagaac tcgcctcatc agaagaagtc agaaaagcat atctaggtgg
    21061 ctaaaacaat ccagtggatt gttttagtcg gcagatgaag attacgaagt aatcatcatt
    21121 atagtctggg agactagttt aggttgcaga taaagattgc gaagcaatca tcaaatccag
    21181 tggattgttt tagtcggcag atggagatta cgaagtaatc atcaatatag tccgggggac
    21241 ctttttagtc ggtagattga gattgcaaac aaatctgcat ctacattgaa agcttaattt
    21301 ctaataattg aaaaaatcga atgaaaaatt tcttaccttc attcacagag ctcgatttca
    21361 gagctctttt tgctagctta ttcatacttt tctgaatttc gaaaaagaaa tgtaagcgtt
    21421 tgatagattt acaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg
    21481 gcagttaaag attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct
    21541 catgcagcag atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat
    21601 caattagttg gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca
    21661 agtctttcta tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg
    21721 attcgcgatg ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg
    21781 ttgaaaaata agattagtat tctccctgtc gtagataacc atcaagtata cggagttatt
    21841 actgaccgtg acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt
    21901 cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt
    21961 gtagaagaaa atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg
    22021 attatcgaag tgcaaatcga tggatcaatt gatttaccag ccttgaaaga aaaatttgaa
    22081 gcaaatggta ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg
    22141 aagcccaaag gcttcttttt tcatgaaaag gggattagag aaaaagatgg aaagaaatga
    22201 taaaatatgc tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc
    22261 gtcagaaaat tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc
    22321 gaagggctag aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg
    22381 aacgataata ttgcagccca aaaaacgtcg caagaattaa atgaattaaa aaacacttac
    22441 aataccttcc ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg
    22501 gctgaagacg agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata
    22561 atgaccagct acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc
    22621 ttggaaatcc atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt
    22681 cgtatgtata ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa
    22741 gcaggtgatg aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat
    22801 ggtctcctca agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct
    22861 gccaaacgtc gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact
    22921 attgaagtgg aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc
    22981 ggtggacaaa acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga
    23041 attgttgtcc aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag
    23101 atgttgcagg ctaagctcta tcaaatggag caagagaaga aggctgcaga ggtagattct
    23161 ctcaaaggtg agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg
    23221 ccttatacta tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt
    23281 atggatgggg acttagatgg ttttatcgat gcttatctca agtggcgaat tagctaagat
    23341 agaaaggaac tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg
    23401 gaacgactgc tctacgcggt gtttcggtta gcgttcaacc gggggaattt gcttacatcg
    23461 taggaccttc aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa
    23521 tcgataaagg aagcctatca gttgctggtt ttaatctggt taagatcaaa aagaaagata
    23581 tcccgcttct acgtcgtagt gttggggttg tcttccagga ttataaattg ttaccaaaga
    23641 aaactgtcta tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata
    23701 tcaaaagacg agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt
    23761 tcccaaatga actctcaggt ggggagcaac agcggattgc gattgcgcgt gcaattgtaa
    23821 ataatcccaa agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat
    23881 gggaaattat gaatcttttg gaacggatta acctacaagg aacaactatt ttgatggcga
    23941 ctcataatag ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc
    24001 gtgtcgttcg tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc
    24061 catttatttg aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc
    24121 agttcagtca tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca
    24181 gcgaaactag ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat
    24241 gtggaagata atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac
    24301 cacaaggtat atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt
    24361 aaagaagaac aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa
    24421 ggagatgcca atcctctcta tgatgcctat attgtagaga caaacactcc aaatgatgta
    24481 aaaactatag ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt
    24541 gccaatacag aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt
    24601 gctgctttgt taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt
    24661 atttcccgca gtcgcgaaat tcaaatcatg cgcttggtcg gagctaaaaa cagttatatc
    24721 cgtggaccgt tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct
    24781 gttttggtct ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg
    24841 caaaatctat ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt
    24901 gtgattgggg ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag
    24961 gtttaggtaa aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga
    25021 gtttttgaaa agagatgcgc agaagaaaag agcttccaaa gaagtccccc agagaagact
    25081 tcttgatggc taatttgaac ttgaaatttg gcgcactag
//
LOCUS       DAYYQN010000029        24934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYYQN010000029 DAYYQN010000000
VERSION     DAYYQN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.029"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            125..823
                     /locus_tag="ACHBRQ_001118"
     CDS             125..823
                     /locus_tag="ACHBRQ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592802.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            820..1467
                     /locus_tag="ACHBRQ_001119"
     CDS             820..1467
                     /locus_tag="ACHBRQ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592803.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1547..2179)
                     /locus_tag="ACHBRQ_001120"
     CDS             complement(1547..2179)
                     /locus_tag="ACHBRQ_001120"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9592804.1"
                     /translation="MELGNQLAQDLGVKVKYISVDAANRAEYLISNKVDITLANFTVT
                     DERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLIVTKGTTAETYFEKNHPEI
                     KLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGFEVGITSLGDPDTIAAAVQ
                     KGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAKADDLVVEGGH"
     gene            complement(2170..2412)
                     /locus_tag="ACHBRQ_001121"
     CDS             complement(2170..2412)
                     /locus_tag="ACHBRQ_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9592805.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYATILN"
     gene            complement(2424..3181)
                     /locus_tag="ACHBRQ_001122"
                     /pseudo
     CDS             complement(2424..3181)
                     /locus_tag="ACHBRQ_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3182..3859)
                     /locus_tag="ACHBRQ_001123"
     CDS             complement(3182..3859)
                     /locus_tag="ACHBRQ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592806.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(3819..4499)
                     /locus_tag="ACHBRQ_001124"
     CDS             complement(3819..4499)
                     /locus_tag="ACHBRQ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592807.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSGK"
     gene            4714..4848
                     /locus_tag="ACHBRQ_001125"
                     /pseudo
     CDS             4714..4848
                     /locus_tag="ACHBRQ_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            4988..6478
                     /gene="lysS"
                     /locus_tag="ACHBRQ_001126"
     CDS             4988..6478
                     /gene="lysS"
                     /locus_tag="ACHBRQ_001126"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9592808.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            7102..8238
                     /gene="lctO"
                     /locus_tag="ACHBRQ_001127"
     CDS             7102..8238
                     /gene="lctO"
                     /locus_tag="ACHBRQ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HFM9592809.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      8492..8580
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8796..9464
                     /locus_tag="ACHBRQ_001128"
     CDS             8796..9464
                     /locus_tag="ACHBRQ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HFM9592810.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFEEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(9432..9650)
                     /locus_tag="ACHBRQ_001129"
     CDS             complement(9432..9650)
                     /locus_tag="ACHBRQ_001129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592811.1"
                     /translation="MKIKEQTRRLTAGCSKHCFEPVDKTDEVSSKHCFEVVDRTDVVR
                     RDFRRVLPHQICKYHEPSLDIVTNIKKS"
     gene            9746..10528
                     /gene="thiM"
                     /locus_tag="ACHBRQ_001130"
     CDS             9746..10528
                     /gene="thiM"
                     /locus_tag="ACHBRQ_001130"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFM9592812.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            10530..11159
                     /gene="thiE"
                     /locus_tag="ACHBRQ_001131"
     CDS             10530..11159
                     /gene="thiE"
                     /locus_tag="ACHBRQ_001131"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFM9592813.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      11368..11456
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11718..12278
                     /locus_tag="ACHBRQ_001132"
     CDS             11718..12278
                     /locus_tag="ACHBRQ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9592814.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            12279..13664
                     /locus_tag="ACHBRQ_001133"
     CDS             12279..13664
                     /locus_tag="ACHBRQ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9592815.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLNGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            13666..14316
                     /locus_tag="ACHBRQ_001134"
     CDS             13666..14316
                     /locus_tag="ACHBRQ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM9592816.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            14327..15019
                     /gene="tenA"
                     /locus_tag="ACHBRQ_001135"
     CDS             14327..15019
                     /gene="tenA"
                     /locus_tag="ACHBRQ_001135"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HFM9592817.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            15024..15548
                     /gene="thiW"
                     /locus_tag="ACHBRQ_001136"
     CDS             15024..15548
                     /gene="thiW"
                     /locus_tag="ACHBRQ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HFM9592818.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            15548..16351
                     /locus_tag="ACHBRQ_001137"
     CDS             15548..16351
                     /locus_tag="ACHBRQ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFM9592819.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            16344..16976
                     /gene="thiE"
                     /locus_tag="ACHBRQ_001138"
     CDS             16344..16976
                     /gene="thiE"
                     /locus_tag="ACHBRQ_001138"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFM9592820.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(17186..17977)
                     /gene="thiD"
                     /locus_tag="ACHBRQ_001139"
     CDS             complement(17186..17977)
                     /gene="thiD"
                     /locus_tag="ACHBRQ_001139"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9592821.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKVFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(18061..18159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18343..18738
                     /locus_tag="ACHBRQ_001140"
     CDS             18343..18738
                     /locus_tag="ACHBRQ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9592822.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            18749..19120
                     /locus_tag="ACHBRQ_001141"
     CDS             18749..19120
                     /locus_tag="ACHBRQ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HFM9592823.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            19130..21373
                     /locus_tag="ACHBRQ_001142"
     CDS             19130..21373
                     /locus_tag="ACHBRQ_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9592824.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDVSQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            21580..23355
                     /gene="spxB"
                     /locus_tag="ACHBRQ_001143"
     CDS             21580..23355
                     /gene="spxB"
                     /locus_tag="ACHBRQ_001143"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9592825.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            23466..23813
                     /locus_tag="ACHBRQ_001144"
     CDS             23466..23813
                     /locus_tag="ACHBRQ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9592826.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(23930..24934)
                     /locus_tag="ACHBRQ_001145"
                     /pseudo
     CDS             complement(23930..24934)
                     /locus_tag="ACHBRQ_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         7406 a         4562 c         5418 g         7548 t
ORIGIN      
        1 ttttcagtat atggtaaatt ccaatccgta tgcattgatt tattggctta cattactatc
       61 tatcataatt ccattgactg tattttctgt tcatagaaac tggaggagag tgtaaaagtt
      121 ggaaatggga aagttaagta gtcacatgtg gagattgaat cagataatct ataccaagta
      181 cttttggggt tatgttcttt tttggatatt gatttgttta ggattatggt attggttaga
      241 aggaaatgat agacttgtta tagaaatttt aaaagggcct aatctgagtc aaaactcttt
      301 tttagtctta tctatatggt tgcttcattg gtttattatt catacatttt ttctagcagt
      361 tgtatatcgt agaagagcat ccgatttctt tctggaagtg attcgatttt cttctattaa
      421 gctctggatt aggtatcaga tttggacctg ttttctttat ggacttattt taatcatggt
      481 aaaagttcta gtgattcaat ttatgttaca gttaccaaac tgggatatag gagttttgtt
      541 tatagttgat tctttgaatg cttgtgtgct atccttgctt tgctttatgt tatacgcact
      601 aggagcgaat gtacaaatga actttgcttg cgttagtttc tttttactca tgattgtgtt
      661 tggtggtcta tttgtaggaa atcgaactaa ttatctattt tatattttga atcgagggaa
      721 tggagatata ggacgagatt tatttcttca gttgctattt ttagtctttc tttttaaaag
      781 cattttctat ttcactcgtc aaaagaggag gtttatagaa tgaatgaaat aattacatta
      841 aaaaatattg agttgaaatt aaaaaaaaca tgtgtttttc aaaaccttaa ctttagttgt
      901 aaacaggggg aaattatagg aattactggt gcgaatggct cagggaaaag tgtattgttt
      961 aaattaatag ctggtttata tagtccgtct tatggagaag tgttaatcaa tggggaaaat
     1021 attgttcctg agagaaaaat tccagctaat ttgggagctt tgattgaaga acctggtttt
     1081 ataaattatt atagtggctt taagaattta caatatttgg caagcatacg aggagtagtt
     1141 ggtaatcagg aaatcaatga tacactgaaa atagttggtc tatatgagca aaaagaccag
     1201 aaagttaaaa cttattcgct aggtatgagg aaaaagctag ggattgctca agcaattatg
     1261 gagaatccct ctattctttt actagatgaa cctatgaatg ccttggataa atcaagtgta
     1321 gaaaatatga gaacattgtt tagaaagctc tctagtgaaa aagggacaac aattttgatt
     1381 gctagtcata gtgaagagga tattcgtatc ttatgtgata aagtatatgc aatagaagat
     1441 aaagtatgta cactgtgttc agattgaaca tcttagatag actcaggatg ttataggtaa
     1501 aatcttaaaa agggattgga gcttaaaatc cagtcccttt taagagttaa tggccacctt
     1561 caacaaccag gtcatctgct ttagcagcgt caccgtaggt tgggtgaagt gttttttcat
     1621 aggccttgtg gaagaagttt tccttgccta atttttcaat atctttattg atgaagtcta
     1681 gcaattcttg gttgcctttt tgaactgctg ccgcaatggt atcgggatca ccgagggaag
     1741 taattcctac ttcaaatcct ttattttcaa gcgcccaagc tagaacttcc gtattgtcag
     1801 ttgaaaaggc atctccacgt ccgtcaagaa gagcttggta agagtcactg tattggtcgt
     1861 atttttggag tttgatttct ggatgattct tttcaaaata agtctcagca gtcgttcctt
     1921 ttgtgacaat taaggtttta ccttcaagtt gtttgacgtc tgtaatgaga ccagtcttag
     1981 gtgatacgac acccagagaa actttcatat atggaagggc aaaatcaact tgtttcttac
     2041 gttcgtcagt tactgtaaag ttagcaagag taatatctac cttgtttgaa atcaagtatt
     2101 ccgcacggtt ggcagcatcg actgaaatgt atttaacctt gacaccaagg tcttgagcta
     2161 gttggttccc tagttcaata tcgtagcgta gccttggtaa gaaccatcat tgtcaacgta
     2221 gccaaacggt tttttatctc caaacacggc gattcgcagt tcaccgcttt ttttgatttc
     2281 atcgatagtg cgagctttgg cagttgtttt tccagaggat gaaccagcgt ttccacctga
     2341 gctacaagca gtgataaaga taagggcaaa ggcaagtgct aaaacagtta agagtggttt
     2401 gaatagtttc ataagaatct cctttataga tatgagccga attggctaaa gtcaaagacg
     2461 tttaaaaatt cctgggctcg tttggtttgc ggattggtaa agaaggcttg agctgttcct
     2521 tcttcagcga ttttcccttg gtcgaggaag ataatccggt cagcaatggc ttgggcaaac
     2581 tgcatttcgt gggttactaa aatcatggta cggccttctt gggccaaatc attgataagt
     2641 tccagcacct cacgcaccat ttctggatcc agcgaagcag tcacctcgtc aaaaaggatg
     2701 atttctggat gcattaggag ggcacggaca attgcaacac gttgcttctg tccaccagat
     2761 aattgacggg caaagctatg ttgtttatcc agcaaaccga cacgatctag taattgcaaa
     2821 gcttcttccg ttacttcttt cttgtccctt ccttgagttt tgatagggcc taggatgagg
     2881 ttttgtaaga catccagatg gggaaagagt tcataacttt gaaagaccat gccaatcttt
     2941 tggcgaacta ggtgaaaatc ttttttattt tcaacgatag actgaccatc cagaagaata
     3001 tctccacctt gaatactttc taagccgttg aggcaacgaa ggagggtact tttcccacaa
     3061 ccagatggcc ctaggatgac aacaacttcc cttttttgat ttctagagaa agtccttgga
     3121 ggatgggatt gtctccgaag gattttttta gttccttgat ttctaagata gtttcagaca
     3181 tttagtttct ccaatgtttt tctaagtgag tggatagttt ggaaataggg tagcaaactg
     3241 cgaaatataa gactagaatg gttccataaa tccaaaatga agcagttggg atggtcaggc
     3301 gattgctatc gatgatttgt tgtccaactt tggtcacttc cacaacccca atcaaaacaa
     3361 ctaatgaagt ggttttaatc atccgagtga caagattgat agcctgcggt agcagtcttc
     3421 ttaagacttg tgggatgatg atgtggtagt aaagttgaac attagtcaag ccgagtgcct
     3481 gtccactttc aaactgatgt ttagggagag aagtgatagc tccacgtacc aagtctccca
     3541 tttcagctgt tccccagagg gtaaaaacga taatagctga agtctcacct gagatattga
     3601 tattaaagtt tcgagctaag ccaaagtaaa cgatgaagag tagcaccagc tggggcatga
     3661 tacgggtaaa gtccagatac aatcgtgtta aaaatcgtat gattctagaa tgggaggtca
     3721 tgatgattcc catgactgtt ccgaacatca tggataagag gacagacagg atggatatct
     3781 caatcgtaac gcccaatccc tgtaagattc tcaggagatt atttccctga aagagtactt
     3841 ggattcccga atcctgcatg gcggagcctc ctttctatcc agctaaagac cagtgagatg
     3901 ggtagcagca tgatcagata agcaactacc aacatagcta gcgcaatgtc tgtctcatag
     3961 tagagaccaa tcaaatcctt ggcgacatac atgaggtcgg ccaaagccac tgctgagaaa
     4021 acagaggttt ccttgataag gaaaatgaca ttggcactaa aggaggggag tgccaccgct
     4081 gttgcttgcg gaagaaccac atagcgaaag acctgtagag gtgtcagacc aatagcgagg
     4141 ccaatctcct gctgggtttg actgatggct tccagcccac ttcggaaaga ttctgccata
     4201 taggatcctc ctaaaaagac aagcccaagc gttgcacaga cttctgaaga taggacaatc
     4261 ccgattcggg gaagaccgaa gtagagaaag aagagttgaa tcaaaagggg cgtattacgt
     4321 gacaattcaa tgtaggctgt cgctacttgc gccaaaacag ggattcgata atgtcggatg
     4381 atactaacga ttaaaccgag cagaaaggat cccaaaattc cccaaactgc aatatgcaag
     4441 gtcagaaaga atgccttttg atatagtggt agatattgtt caacaatgga ccaatccaaa
     4501 aatagaacct cccatctaga aataatacag ttattgtagc acttaaaatc ttctttggat
     4561 aatatctatt ttttattgcc gttataagga ttttttacca tagacataaa atttctgaaa
     4621 tttccaaaca aaatatttga aaagttttga aaaaaagtta agatattttt gtaatataca
     4681 aagtaaacgc ttacttatta aggaggacag tttatgtcat acaaaacaag caatgcagaa
     4741 ggtcatgtag atttcatcaa tacctatgat ttggagacaa tggcgcaaca agttattcct
     4801 aaagcagcat ttggctatat cgctagtggg gcgggagata ctttcacttc tttccagtga
     4861 ttttagcgtc aggttctttt tagtttttaa agattatccg tgaatttctt gcttatttat
     4921 gataaaatgg gagtatcgca aaaaatgact catcgtattc aattttgagt aaaactagga
     4981 ggatcccatg tctacagaac atatggaaga actaaatgac cagcagatcg ttcgccgtga
     5041 aaaaatggct gcgctccgcg aacaaggaat cgatcctttc ggaaaacgtt ttgaacgtac
     5101 tgcaaattca caagaattaa aagataaata tgccaacctc gataaagaac aattacacga
     5161 taaaaacgaa acagctacta tcgcaggacg cttggtaacc aaacgtggta aaggaaaagt
     5221 tggttttgcc caccttcaag accgcgaagg ccagattcag atctacgttc gtaaggatgc
     5281 tgtcggtgaa gaaaactacg aaatcttcaa aaaagcagac cttggtgact tccttggtgt
     5341 cgaaggtgaa gtgatgcgta cggatatggg agaactctct atcaaggcaa cccacatcac
     5401 acacttgtct aaggctcttc gtcctcttcc tgagaaattc catggtttga cagacgttga
     5461 aacaatttac cgtaaacgtt accttgactt gatttctaac cgtgaaagct ttgaacgctt
     5521 tgtcactcgt tcaaaaatca tctctgaaat ccgtcgttac cttgaccaaa aaggattcct
     5581 tgaagtggaa acacctgttc ttcataatga agccggtggt gctgctgccc gtccatttat
     5641 cacccaccac aatgcccaaa acattgacat ggtgcttcgt atcgcgactg agcttcactt
     5701 aaaacgcctt atcgtgggtg gtatggaacg tgtctatgaa attggccgta tcttccgtaa
     5761 cgaaggaatg gacgctactc ataaccctga gtttacttct atcgaagttt accaagctta
     5821 tgcagacttc caagacatca tggacttgac ggaaggcatt atccaacacg ctgctaaatc
     5881 agttaagggc gatggcccag tcaactacca aggtactgaa atcaagatta acgaaccatt
     5941 taagcgtgtt catatggtgg atgctatcag agaaattact ggtgtcgatt tctggcaaga
     6001 catgacttta gaagaagcta aagctatcgc tgctgagaag aaagttccag ttgagaaaca
     6061 ctacactgag gttggtcaca tcatcaatgc cttctttgaa gagtttgttg aagaaacttt
     6121 aatccaacca acctttgtct atggacatcc agtagctgta tctccactcg ctaagaaaaa
     6181 tcctgaagac caacgcttta ctgaccgttt cgagctcttt atcatgacta aggagtacgg
     6241 taatgccttt actgagttga acgacccaat cgaccaactt agccgttttg aagcccaagc
     6301 taaagctaaa gaacttggtg atgatgaagc gacaggcatc gactatgact acatcgaggc
     6361 tcttgaatac ggtatgccac caacaggtgg tttgggaatc ggtatcgacc gtctctgcat
     6421 gctcctcact gatacaacaa ctatccgtga tgtattgctc ttcccaacaa tgaaataaat
     6481 tcttatcctc tggctcttac cagaggattt tttgattcaa aaagagactg aatttaagga
     6541 gaaaatgaag tgtagtatat tgaagttgaa atagtacact ttgatttcta agacattgtt
     6601 agaaattggt ttaaattccc taagcaattt gtgcatgttt tatttcattt tacgatagta
     6661 cgctgaaact tttcaaaaag tactagaaat tgacttggat tccccaattg atttgttcag
     6721 attcactata aataaaaaat taataagtgg gataggaagt tagcgtcaac taggatagta
     6781 tcttgcttaa acagtatata tgggactgat ataagtccat aggtcctatt agaggatgtt
     6841 ctggtgtctt attcacttgt tttttatagt attagtagat agaatcagca aataaaaaag
     6901 tagttcaaat gcctggcagc aaaaaatctc aacgattcac ttaatataga ctaaggttta
     6961 gagtttaagg gaagtagggc tagtatattg tatgaaaaaa taagatggtt tttaagcaaa
     7021 actcttgaaa atgattgaaa aaagagttat tatagttgta tagtaaaaag caaacgctta
     7081 cttaatcgag gaggacagtt tatgtcatac aaaacaagca atgcagaagg tcatgtagac
     7141 tttatcaata cctatgattt ggagccaatg gcgcaacaag ttattcctaa agcagcattt
     7201 ggctatatcg ctagtggggc ggaagatact ttcactttaa gagagaatat ccgcgccttt
     7261 aaccacaaac tcatcgttcc tcatacactt tgcgatgttg aaaatccaag tacagagatt
     7321 gaatttgcag gtgaaaaact atcttcgcca atcattatgg cacctgttgc ggcccataaa
     7381 ttggcaaatg aacaggggga agtggcgact gcgcgtggtg tgcatgagtt tggttctctt
     7441 tatacaacca gttcttactc tactgttgac cttccagaga tttctgaagc ccttcaaggg
     7501 acacctcatt ggtttcaatt ttactttagt aaggatgacg gtatcaaccg ccacatcatg
     7561 gaccgtgtga aggctgaagg ttataaagcg attgtcttga cggcagatgc tactgtaggg
     7621 gggaatcgtg aagtggataa gcgtaatggt tttgtcttcc cagttggcat gccgattgtt
     7681 gaagaatacc tgccagaagg tgctggtaaa tcaatggact ttgtttacaa atcagctaaa
     7741 caacgcttgt ctccacgcga tgtagaattt atcgctgaat actctggtct tcctgtgtat
     7801 gtcaagggac cacaatgccg tgaggacgtt gaacgttcgc ttgctgcagg agcttctggt
     7861 atctgggtaa ccaaccacgg tggtcgtcaa atcgacggtg gaccagctgc ctttgactcg
     7921 cttcaagaag tggcagaagc agttgataga cgtgtgccga ttgtctttga ctctggtgtt
     7981 cgtcgtggtc aacacgtctt taaagccttg gcatcaggag cagacttggt tgctattgga
     8041 cgccctgtca tctatggctt ggctctcggt ggtagtgtcg gtgtgcgtca agtctttgaa
     8101 cacttgaatg cggaattgaa gacagttatg caattgtctg gagctcagac cattgaagat
     8161 gtcaaacact tcaagctccg tcacaatcca tacaacccaa ccttcccagt tgaccctcgt
     8221 gacttaaaat tgtattgata aaacagattg cctccactga atgtggaggt ttttcttcgt
     8281 gtattggaag aataaatcgc tatcgaaaac tatagtaaaa tgaaataaga ataggacaaa
     8341 tcaatcagga cagttaaatc gatttctaac aatgttttag aagtagaggg gtactattct
     8401 agtttcaatc tactatatat aaagatattt ttcggttaaa tatcttgcta aggctttcat
     8461 tttttgctat actggttcag taattaaata atagagacat ttggggtgct ttaggctgag
     8521 ataataccca ttgaacctga tacagttaag actggcgaag ggaaatgtga aacgtttttt
     8581 cgtatggcga aatgaacgat ctaatcttgt aagtcaactc taattcttga ttgtaattgg
     8641 agttttttgt ttatataaac tggataggct tgtttagtta gcttctctgc aaatgctctc
     8701 gatgtgcttt aaaaggatca agtggaatta cgattttgct tgatatttct tggtgtttta
     8761 ttaattacta acaagtaaag agaggagaag taaatatgga aacacaagac tatgcatttc
     8821 agccaggttt gactgttgga gaattattaa aaagcagtca gaaggattgg caggctgcaa
     8881 tcaatcatcg ttttgttaag gaactttttg cggggacaat tgagaataag gtcttaaagg
     8941 actatctgat tcaagattat cacttctttg atgccttctt atccatgctg ggtgcctgcg
     9001 tagctcacgc agataaactt gaatccaagc ttcgttttgc caagcaacta ggctttcttg
     9061 aagcagatga agatggttat ttccaaaagg cttttaaaga gttaaaagta gcagagaatg
     9121 actatctaga agtgactttg caccctgtaa caaaagcctt tcaggatcta atgtattcgg
     9181 cggtggcttc atcagactat gcccatcttt tggtcatgct ggtcattgca gaaggtctct
     9241 atttagactg gggttctaaa gatttggctt tacctgaagt ctatattcat tcggaatgga
     9301 tcaatctcca cagaggtcct ttctttgaag agtgggttca atttctggtt gacgaactca
     9361 atcgtgtcgg taaaaatcga gaagatttga cagaacttca gcaacgctgg aatcaagcag
     9421 tcgctttaga attagctttt tttgatattg gttacgatgt ctagagaagg ctcatgatat
     9481 ttacaaattt gatgaggtaa gactcttcga aaatctcttc gaaccacatc agttctatcc
     9541 acaacctcaa aacagtgttt tgagctgact tcgtcagtct tatctacagg ctcaaagcag
     9601 tgttttgagc aacctgcggt tagcctccta gtttgctctt tgattttcat tgagtataaa
     9661 tcattcaatg taaatgaaaa aaattctcaa ctaaagataa atcgaaatca gtagatcgta
     9721 taaagtgaaa aggaaggatt tcaatatgac aagtttaaaa ttattaaaag aaaaagcacc
     9781 attggtcatt tgtatcacca atgatgtagt aaaaaatttc acagcaaatg gattagtagc
     9841 actgagcgtc tcaccagcca tgagtgagtt tccagcagat ttagaggatt tgttaaagta
     9901 tgctggtggt ttattaataa acataggaac attgacagat gaaaattgga aattatacca
     9961 agctgctctg aaaattgcgg agaaatataa tgtcccagca gttttagatc ctgtagcctg
    10021 tggagcagga gaatatagaa aaaaagtagc agatgatcta atcaacaatt ataaactagc
    10081 agcgattaga ggaaatgctg gcgagattgc ctctttagta ggaatagatg tggcatctaa
    10141 aggagtagat agtgcgggcg tagataatat tgacgaaatt gctctagcag caaatgagaa
    10201 gttcaatatt ccaatagtag taacaggtga agtggatgcc attgctgtta atggagaagt
    10261 ggtaacgatt cataatggta gtgctatgat gccgaaagtc attgggacag gatgcttatt
    10321 aggagctgta gtagcaagct ttatcggact agaaaaaggt caagaattga aatcattaga
    10381 aacagcaatg ttggtttaca atatcgctgg agaaatggca gaaaaacgtc caaatggaca
    10441 tcttcctggg acatttaaag ttgaatttat aaatgcctta tacgagatta cagatgaaga
    10501 tgtaaaggaa ttcaaaagag tgaagtaaga tgtttcataa agaattacta aaactatatt
    10561 ttatttgtgg aacgactact tgccaaggaa aaaatctata tacagtcgtt gaggaagcct
    10621 taaaaggtgg tataacctta tttcaatttc gagaaaaagg tgagggtgct ttagaaggct
    10681 tagaaaaact tgagctagct atccaaatta aagaactttg taaaaaatac aatgttccat
    10741 ttatcgttaa tgacgatata gatttagcaa tggaaattga cgctgatggc gtacatgtag
    10801 gtcaagatga tattggtgtt gatgaaatta gaaaattgat gccagataaa ataattggtc
    10861 tttctataaa aaacgaggaa gaatttcaac aatcaaaagt tgaatatgta gattatgttg
    10921 gtgttgggcc tgtatttgat acccagtcaa aggatgatgc tggtggtgct ataggttatg
    10981 aaggtgttaa attgatgaga aaactgttgc cacaaatgcc cttagttgca attggtggga
    11041 tacagacgaa acatattaaa gacattataa agaccaatat ggatggcgtt tcaatcattt
    11101 cggcaatatc gtatgcaaaa aatatagaaa aaactgttcg ggaaatgagt gaacaataga
    11161 tatctatcct tggattctaa gtaagtagga ttttcctttt ttaaaaagct atgtctagat
    11221 tcagcttgaa tggctaattc atcaaacagg tcatttgttt tttgcaaaag aaagatctgc
    11281 tgtctttgtg agtgcaaggc tgtatttcca gttcaatttc ttgaactagt ataaaatgtg
    11341 tgttataata atcacataat taaataataa agacatttgg ggtgctttag gctgagatga
    11401 tacccattga acctgataca gttaagactg gcgaagggaa atgtgaaatg tttttttcgt
    11461 atgtcgaaaa gaactgtcta atcttgtaag ctgaactcta attcgtatct gtaattagag
    11521 ttttttcttt tacgaaactg gatgaagatg atatagtaga ttgaaataag atgcgaacaa
    11581 atcaattaga aaattcaaat taatttctag aaatatttta gcattcttgt gtactgttct
    11641 aaattcagtc tgctatacaa agtatttagg tcggtcttct tgagtgcccc acttttcttt
    11701 aaaaaaagga gttttttatg ttgaaaaaat ggcagttaaa agatgttatc ttgcttgctt
    11761 tcttgtctat cttttttggt ggggttttcg ttggttcagg atatgtgtat aatattctca
    11821 gtctactctt aacacctctt ggtttgcagg cctttgccaa tgaaatcctc ttcggtctct
    11881 ggtgtatggc tgcgcccatt gctgccatct ttgttccgag agtcggaagt gcaacgattg
    11941 gagaagtgct agctgcgctt gctgaagtcc tttatggtag ccaatttggt ctaggagctc
    12001 ttttgtctgg cttggttcaa ggtttgggaa gtgaatttgg ttttatcgta actaagaatc
    12061 gctatgaaag ttggctctct ctaactgcta atagtattgg gattacgctt gttagctttg
    12121 tctatgaata cattaagtta ggttactacg ccttttccct tccgtttgtc ctttccttac
    12181 ttgtggtacg ttttatttct gtttatttct tctgtaccat cttggttcgt gccattgtca
    12241 aactctatca tcagtttgca actggaggaa aagcatagat gggtctggaa ctacgagcga
    12301 ttcagtcccc aatcttctct gagccgtttg attttacttt tcatgcgcaa gcctttacct
    12361 tgttagttgg gagtagtggg tctggaaaat ccagcctctt tcaaatgatt gcccaagtca
    12421 gttctcttcc ctatagtggt caagtcctga tagatgggag cgaggtcagt caactttcta
    12481 tcatcgcacg tgtccagaag gttggcattc tctttcaaaa tcccaatcat caatttacca
    12541 tggagagctt gtttgaggag ctgattttca ccttggagaa tatcggctat caccttcagg
    12601 aaattgattc taaaatagca gaggttgtcc agcaatgtcg ttgcgaggca atcttacacc
    12661 gtcccattca tcacctatca ggtggggaaa agcaaaaagc agcgctggct gtcctctttg
    12721 ccatgaatcc tagggtctat ctcttggatg aaccttttgc ttccattgac cgcaagagca
    12781 gaatcgagat attggagatt ctaaaagagt tggctcttga tggaaagaca gttattttgt
    12841 gcgaccatga tttatctgac tataaagcct atatcgacca tatggttgag ctaagagacg
    12901 gaaaactaag ggaagtgttt caaatccctt cctatgagat gacacaggtt gcttcaaagg
    12961 aagttgcttc tagcccggaa ctattccata tggatagaac gacttgtgag tttggaaatc
    13021 gccccctctt ttcaattgct gatttcacat tctatcaagg gatttcctgt atcctgggtg
    13081 acaatggtgt cgggaaatca accctctttc ggtctattct tcaatttcaa aagtataagg
    13141 ggcgcattgc atggaagggt tcggtcctga aaaagaaaaa gagtttgtat cgtgatctga
    13201 ctggtgttgt tcaggaagct gagaagcagt ttatccgagt cagtctgcga gaggagcttc
    13261 aattagatgg acctgattct gaaagaaatc agcggatttt tcaagcttta cgatattttg
    13321 atttggagca ggcagtcgat aagagtccct atcaattaaa tggtggtcag caaaaaattc
    13381 ttcagctcct gaccatcttg accagtaagg cttccgtgat cttgctagat gaaccttttg
    13441 caggtttgga tgatagagcc tgccattatt tttgcaagtg gattgtggag gagaggaatc
    13501 aaggaagaag ttttctgctc attagtcatc gtttagaccc tttgatttct gtggttgatt
    13561 attggattga gatgactagt cagggtcttc ggcatgtgaa agaagtgacc attaccaaac
    13621 cacttacatc tcagagtagc aatacccaag gggaggtgag atagtatggt caaagtagca
    13681 acccagacac cgattatcag tctcttcttg ctgattttat ccttggaaac atctttcatt
    13741 ccttcgattg ctctgaatct ttcggtagtc gcattttgta ttctctttat gctctattac
    13801 cgtcgattta aaatgttagc ttggatgatc atacttgcca ttttaccatc ttttgccaac
    13861 tactgggcag ttcagttaca cggagatgct tcacaggcag tcatgcttgg aacgagggcc
    13921 tttgtgacag tttgtatcgg ccttgtcttt gtttcctcta tttcactaaa agagcttctc
    13981 ttgtacttgg ctcaaaaggg gctatcacgc tcttggtcct atgccttgat tgtggtattc
    14041 aattcttttc ctctcattca gcaagaaatc aagtccctca aagaagcttg cctattacgt
    14101 ggtcaagaac tacatttttg gtcgcccttg atttacagta aggttctgat gacagtcttt
    14161 aggtggcgcc atctttacct gagagctcta tctgctcacg gatatgacga acatgcacag
    14221 ttgaagaata gctatcggac tttttatatt cctaaaaaaa caaaattaat ctacctgctt
    14281 ttctttttat tgcttcaaac cagtctattt ttataaagga gttattatgg aatttacaga
    14341 tattgcgatg gaattatcca agaaagcttg gcaggcttcc tttcatcatc cctttatttt
    14401 acaattacaa gaggggaatt tagaacctgc cattttccgc tattacctga ttcaggatgc
    14461 ctactatctg aaggctttct cagaaatcta tcatcttttg gctgataaga cttcaaacca
    14521 agagatgaaa agactcttga aacaaaatgc tcagggttta gtggagggtg agttatttat
    14581 tcgccaacaa tttttcaagg aattggaaat cagcgaccag gaaatggagc aacatccaat
    14641 cgctccaacc tgttatcatt acatttctca tatttatagg caatttgcag aaccgaactt
    14701 agccatcgct tttgcaagct tgcttccttg tccttggtta taccatgata taggcaaatc
    14761 acttaatctt aaaccatcac caaatcctct ctatcaacaa tggattgaaa cttatattac
    14821 ggatgagtta gagcaacaga tcagagagga gggagcactg gtcaatcagc tctatcgaga
    14881 aagtgatgag acagataagc aaaaaatgct agatgccttc cacatcagtg ttcatatgga
    14941 agccaagttt tgggagatgg cttaccaaca ccagacatgg aagagcgatt tacagtcttt
    15001 agaaaaagga gaagaataga aacatgagaa agcaccaact acaagttcac aaattgacca
    15061 ttttatctat gatgattgcc cttgatgtag tccttacacc tatctttcgg attgagggaa
    15121 tggcaccgat gtccagtgta gtcaatattc tagcgggaat catgatggga cctgtttatg
    15181 ccttggctat ggctacagtg acagccttta tccgtatgac gactcaaggg attccgcctt
    15241 tagctctcac aggagcgact tttggagccc ttctagcagg tctcttttat aagtacggtc
    15301 gaaaatttta cttttctgcc ttgggagaaa ttgtgggaac aggtattatt ggttctattg
    15361 tttcctatcc tgttatggta ctctttacag gatcagctgc taagcttagc tggtttatct
    15421 acacgcctcg atttttcgga gcaaccttga ttggtacagc gatttccttt attgcctttc
    15481 gatttttaat caagcaggaa ttctttaaaa aagtgcaggg atatttcttt agtgaaagga
    15541 tagactgatg caggaattta caaatccctt tcctataggc tctagttccc tcattcactg
    15601 tatgaccaat gagatttctt gtgagatgct ggcaaatggg attttggctc tgggatgcaa
    15661 acctgtcatg gcagatgatc cccgtgaggt tcttgatttt actaagcaaa gtcaggctct
    15721 cttcatcaat ttggggcatt tgtcagctga gaaggagaaa gcaatccgta tagcagcttc
    15781 ttatgcagct caagtttgtc tcccaatggt agtagatgcg gttggcgtaa cagcttcatc
    15841 cattcgtaag agcttagtca gagacctttt agactataga cctacggtcc ttaaaggaaa
    15901 catgtcagaa attcgaagtc ttgttggatt aaagcaccac ggcgttgggg tcgatgcgag
    15961 tgctaaagat caagaaacgg aggatttgct tcaagtcttg aaagactggt gtcagaccta
    16021 tcatggtatg tctttcttag tcacaggtcc caaggacctc gtcgtttcga aaaatcaggt
    16081 cgctgtactg ggaaatggct gtgcagaatt agactggata acagggacag gagatttggt
    16141 tggagcctta acagccgttt ttctcagcca aggaaagact ggttttgaag cttcttgctt
    16201 agcagtctct tatctcaata tcgctgctga gaaaatagtt gttcaaggaa tgggattgga
    16261 agaatttcgt taccaagtac tcaatcagct ttcgctccta agaagagatg aaaattggct
    16321 agataccatc aaaggagagg tttatgaata gagaagcact tagactatat ctggtaacca
    16381 atcgctatca agattccgtg gaaagctttc ttgcaaaagt tgagacggcc tgccgttcag
    16441 gggttaccat agtccaattg cgagaaaaaa atctcacaac caatcaatat tatcaactgg
    16501 caaaacaagt caaggaaata acagatgctt atcaggtacc cttgataatc gatgatcggt
    16561 tggatgtttg tcttgcggtt gatgctgctg gtctgcatat tggagatgac gaactcccag
    16621 tttcggttgc ccgacaagtc ttgggtcctg aaaaaatcct cggtgtcacc gctaaaacgg
    16681 ttaaaagagc tctggaagcg gagaaatcag gtgcagatta cttggggaca ggagccattt
    16741 ttccgacaac caccaaggaa aatgcaccca tcaccctgat ttcaaccttg aaaacaattt
    16801 gccaaacggt tgccattcca atagttgcta ttggtggctt gacttcggag aatattgatc
    16861 agcttatggg cactggcata gctggtgtag ctgttgtacg tgatttgatg caagcagaag
    16921 atattgaggc aaaaacgcaa gcctttttaa caaagttgca tgacattctt tcctaattaa
    16981 tactcaatga aaatcaaaga gtaaactaga aagctagtcg caggttgctc aaagtactgc
    17041 cttgaggttt tagataagac tgacgaagtc agctcaaaac agtgttttga agttgcagat
    17101 ggaagctgac gtagtttgaa gagattttcg aagagtataa aaactctcta attcccttaa
    17161 agtgggaact agagagtttt ttttcttaat ctttaaaagt tgtatggttg actggaccag
    17221 aaccatgacc gagttgaggg gcatcttgaa tagcttttgt gataaagacc ttggccttat
    17281 caactgcctg gtaaagactc ttgcccttgg ctagttcagc agtaatcact gcagcaaagg
    17341 tacatccagt accatgggtg tgacaggttt gaattcgtgg gctttcccag acaaattgtt
    17401 cattcttggt aaagaggaaa tctttagcac cacctttgag atgtccgcct ttgataacca
    17461 cagactgagg accaaattct tttaaaatca ggcgaccagc acgctgcatg tcttcggggt
    17521 catggattga aaaaccaaca atctcttctg cttcaggaag atttggcgta ataatagttg
    17581 ctagaggtag taagtttgtt ttgagatagt ctctagcact tgagtcaatc aaggcatctc
    17641 cacttgtagc aaccataaca ggatcaagga catagggaca atccagtttt ttaagatagg
    17701 gttggatgat ttccatgatt tcagtagtag ccaacattcc agtttttaca gcctgaggtg
    17761 gaatatcaga aaagacactc tccaattggg ctttcaacat ttgaggagaa acgtgctcga
    17821 ttagctgaac acctctggta ttttgagcga caagactggt tacaacagcc attccataga
    17881 catctctcgc ttggaatgac tttaaatctg ccataatgcc agcaccacca ctagggtcag
    17941 tccctgcaat ggtcaaagca acgggtaaat aagtcatcta aaacctccca accaactgaa
    18001 gtttgtattc ttaagaacaa gctagtctgt tttagaaagg caatagaaga ctgctagtcc
    18061 ccattatcat ttccacttcc atcagctagc attacctaga ttgagtcaga gggatctaac
    18121 agtcgttaat ctcagcttta cgcaccccta gtggttgttt tcttttttct ttagtatagc
    18181 atatttttat tgtttatata gtaatatttg actgcaaatc gtttttcaaa tgattaaaat
    18241 tctatttttc gaagaaaata ttatttctat aattgacaaa tgtagatttt gggagtatac
    18301 tgttgagtgt aattgacaaa tgtagatttt ggaggtatgg taatgcagat ttcagatgca
    18361 gaatggcagg tcatgaagat tatttggatg cagggggagc agaccagtac agatttgatt
    18421 agggttttgg cagagcggtt tgactggtcc aagtccacgg ttcagacact tttggctcgt
    18481 ctagttgaga aagagtgttt gactcggaaa aaagaaggca agtcctttgt ttattcagcg
    18541 cttttaactc tagaccaaag tcgggattta cttgtccaag atatcaaaga caaggtttgt
    18601 tcccgtagga ttaggaattt gttggctgac ttgattgtag aatgtgaatt tactcagact
    18661 gacttggaag acttggaagc tgtgatttca gagaagaaat caagtgctgt aacagaagta
    18721 agatgtaatt gtatgtaaag gagacgtcat gttaaatagt attgtaacca ttatttgtat
    18781 tgcccttatc gcgtttatct tgttttggtt tttcaaaaag cctgaaaaat ctggacaaaa
    18841 agcccagcaa aaaaacggat accaagagat tcgagtggaa gtcatgggag gctatactcc
    18901 tgagttgatt gtcctcaaga aatcagtgcc agcccgcatt gtctttgacc gcaaggatcc
    18961 ttcaccatgt ctggatcaaa ttgtttttcc agattttggt gtacatgcga acctgccaat
    19021 gggggaagag tatgtagtgg aaatcacgcc tgaacaggct ggagagttta gctttgcttg
    19081 tggtatgaac atgatgcacg gcaagatgat tgtagagtag gtggagacta tgacagaaat
    19141 tgtgaaagca agcttagaaa atggcattca aaaaatccgt atccaagctg aaaaaggcta
    19201 tcatccagcc catatccagc ttcaaaaggg aattccagct gagattacct ttcatcgtgc
    19261 tactccttca aactgttata aggaaattct gtttgaagaa gaaggtatct tggaaccaat
    19321 cggcgtagat gaggagaaag tcattcgttt tacacctcaa gaattagggc gacatgaatt
    19381 ttcttgtggc atgaagatgc aaaagggaag ctatacagtc gttgagaaga ctcgaaaatc
    19441 tctatctctc ctgcaacgtt tttggattac tagtatctgt actgtgcctc ttgtgattct
    19501 catgattggg atgttggcag gtagcattag tcatcaagtc atgcattggg gaacctttct
    19561 agcaacaacg cctattatgt tagttgcagg taagccatat atccagagtg cttgggccag
    19621 ttttaaaaag cacaatgcca acatggatac cttggttgcg ctgggaactc tagtggctta
    19681 tttctatagc ctagttgctc tctttgctgg tctccctgtt tacttcgaaa gtgctggatt
    19741 tatcctcttt ttcgttcttt tgggagcagt ttttgaggaa aaaatgagaa aaaatacgtc
    19801 ccaagctgtg gagaaattac tggacttgca agctaaaacc gcagaagtct tgagtgatga
    19861 tagttatgtc caagttcctt tggaacaagt caaggtaggc gacctgattc gagtgcgtcc
    19921 cggtgaaaag attgctgttg atggtgtcgt agtagaaggt gtctctagta ttgacgaatc
    19981 catggtgaca ggtgagagtc tgcctgtgga caagacagtt ggagatactg tcattggctc
    20041 aaccatcaat catagtggaa cgcttgtctt tagagcagaa aaagttggct cagagactgt
    20101 tttggctcag attgtggatt ttgtgaagaa agctcagaca agtcgtgcgc cgattcagga
    20161 cttgacggat aagatttcag ggatttttgt cccagtagtt gtcattttag gaatcatgac
    20221 cttttgggtt tggttcgtct tgctcaggga tagtgtggtt gtgcttggag ctagctttgt
    20281 gtcctctctt ctctacggag tggcggtttt gattatcgcc tgtccttgtg ccttgggact
    20341 tgcaacaccg acagccctta tggtggggac aggacgtagt gccaagatgg gggttctcct
    20401 caaaaatgga actgtcttac aggaaatcca gaaagttcaa acccttgtct ttgataagac
    20461 cgggactttg acggaaggga aacctgtggt aacagatgtc atcggcgacg aagtagaagt
    20521 gtttggattg gcagcctcct tggaagatgt ttctcaacac ccactggctg aggctatcgt
    20581 taagcgagcg agtgaagctg gacttgagtt tcaaactgtt gaaaattttc aggccttgca
    20641 cgggaaaggt gtttcagggc gaatcaatgg aaaacaagtt ttacttggaa atgctaaaat
    20701 gctggatggc atggatattt ctaatactta tcaagataaa ctagaagaac tagaaaaaga
    20761 agctaagaca gttgtgttct tagctgttga caatgaaatc aaaggcttgc ttgctttgca
    20821 agatattcct aagaaaaatg ctaagctagc catcagtcag ctaaaaaaac gtggtctcgg
    20881 gacagtcatg ctgacaggag acaatgctgg tgtggcgcgt gctattgcag atcaaatcgg
    20941 aattgaagag gtcattgcag gcgtcttgcc agaagaaaaa gcccatgaaa tccataaact
    21001 gcaacagtca ggtaaagtag cctttgttgg tgatgggatc aatgacgctc ctgcccttag
    21061 tgtagcagat gtggggattg ctatgggagc tggaacagat atcgccatcg agtcagcaga
    21121 tttggtgttg acaaccaata atcttttagg agtggtgcgt gccttcgaca tgagtaagaa
    21181 aacctttcat cgtattctgc tcaatctttt ctgggctttt atctacaatg ttgtcggaat
    21241 tccgattgca gcaggagtct tttcaggtgt tggactggct ctcaacccag aattggcagg
    21301 tctagccatg gcctttagtt ctgtatctgt tctgaccagt tcccttctgt taaattttag
    21361 taaaatagac taaaagtaat gactagattt ttttgttata aaacagaaat gacgacactt
    21421 tcaaaactgt attagaatag agaatagaga gttttgagca gatttttaga aaagtcagca
    21481 taaatatgat acagtggaat agtaaaaatt tggagaacgt ttccaattct atgtaatcgt
    21541 attctccaag tctaaaaaaa ttgaaggaga gttatcatta tgactcaagg gaaaattact
    21601 gcatctgcag caatgcttaa cgtattgaaa acatggggcg tagatacaat ctacggtatc
    21661 ccatcaggaa cactcagctc attgatggac gctttggctg aagacaaaga tatccgcttc
    21721 ttacaagttc gccacgaaga gacaggtgct cttgcagcgg ttatgcaagc taaattcggc
    21781 ggctcaatcg gggttgcagt tggttcaggt ggtccaggtg cgactcactt gattaacggt
    21841 gtttacgatg cagctatgga taacactcca ttcctagcaa tccttggatc acgtccagtt
    21901 aacgaattga acatggatgc tttccaagag cttaaccaaa acccaatgta caacggtatc
    21961 gctgtttaca acaaacgtgt agcttacgct gagcaattgc caaaagtaat tgacgaagcc
    22021 tgccgtgctg cagtttctaa aaaaggtcca gctgttgttg aaattccagt aaacttcggt
    22081 ttccaagaaa tcgacgaaaa ctcatactac ggttcaggtt catacgaacg ctcattcatc
    22141 gctcctgctt tgaacgaagt tgaaatcgac aaagctgttg aaatcttgaa caatgctgaa
    22201 cgcccagtta tctatgctgg atttggtggt gttaaagctg gtgaagtgat tactgaattg
    22261 tcacgtaaaa tcaaagcacc aatcatcaca actggtaaaa actttgaagc tttcgaatgg
    22321 aactatgaag gtttgacagg ttctgcttat cgtgttggtt ggaaaccagc caacgaagtg
    22381 gtctttgaag cagacacagt tcttttcctt ggttcaaact tcccatttgc tgaagtttac
    22441 gaagcattca agaacactga aaaattcatc caagttgata tcgaccctta caaacttggt
    22501 aaacgtcatg cccttgacgc ttcaatcctt ggtgatgctg gtcaagcagc taaagctatc
    22561 cttgacaaag taaacccagt tgaatcaact ccatggtggc gtgcaaacgt taagaacaac
    22621 caaaactggc gtgattacat gaacaaactc gaaggtaaaa ctgagggtga attgcaattg
    22681 tatcaagttt acaatgcaat caacaaacat gctgatcaag acgctatcta ctcaatcgac
    22741 gtaggtaaca ctactcaaac atctactcgt caccttcaca tgacacctaa gaacatgtgg
    22801 cgtacatctc cactctttgc gacaatgggt attgcccttc ctggtggtat cgctgctaag
    22861 aaagacaatc cagatcgcca agtatggaac atcatgggtg acggagcatt caacatgtgc
    22921 tacccagacg ttatcacaaa cgttcaatac gaccttccag ttatcaacct tgtcttctca
    22981 aatgctgagt acggcttcat caagaacaaa tacgaagata caaacaaaca cttgtttggt
    23041 gtagacttca caaacgctga ctacgctaaa attgcggaag ctcaaggagc tgttggattc
    23101 acagttgacc gtatcgaaga catcgatgca gttgttgcag aagctgttaa attgaacaaa
    23161 gaaggtaaaa ctgttgtcat cgatgctcgc atcactcaac accgtccact tccagtagaa
    23221 gtacttgaat tggatccaaa acttcactca gaagaagcta tcaaagcctt caaggaaaaa
    23281 tacgaagcag aagaactcgt accattccgt ctcttcttgg aagaagaagg attgcaatca
    23341 cgcgcaatta aataattcct ctcgccgaaa atcaaatatg aaacttgtag actttattct
    23401 ttgattttca tagagcagaa ctttaaaaga tcggagcaat ccggtctttt tatggaggag
    23461 agtaaatgaa tttaaatcaa ttagatatta tcgtttcaaa tgttccccaa gtctgtgctg
    23521 acttggagca tattttggat aaaaaggcag attatgctga tgatggtttt gctcagttta
    23581 cgattggtag tcactgcctt atgttgtcac aaaatcattt ggttcctttg gaaaactttc
    23641 agtcaggaat cattattcat atcgaggttg aggatgtaga ccagaactac aaacggttga
    23701 acgagcttgg tataaaggtt ttacacggac caactgtaac cgattgggga acagagtcct
    23761 tattagttca aggacctgct ggtctagtgc ttgattttta tcgtatgaaa taggtcgcgt
    23821 agtcatttga cgttaacctt agctattttt aaaatagaaa ttgagaatat gaactattga
    23881 aagatcggag caatccggtc tttttatata aagaaattgc atataatact catatgcttt
    23941 caaaaagcat tctagtccat ctccgattaa cgatggactt tatcacctcc ttctccagtc
    24001 cttgtatgac atcttgaagt tgattcatga catcttccaa agttcgaaag gccttattct
    24061 taaatccacg tttacgaatc tctttccaca cttgttcaat gggttcatct ctggtgtgta
    24121 tggaggaata aaggtaaaac caatattagt cggaatcttt aaggtacttg atttatgcca
    24181 tatagcattg tccataacga gtaaaagata atcatctgga taagcttgtg aaagctcttc
    24241 taaaaaggcg ttcatccact cagtattaca tccaccagct attaagaaaa atgattcgcc
    24301 tgtatgggca tcaacagctc cataacaata gtgaaattct cgtatatagt gactatggac
    24361 atgtggacct actcctattg gagcccaaca agatcccagt ttactgattc taccgaaacc
    24421 agcctcatct tggtacatca agcgaacctt acgaaaacgt ctactgcttt taaagcgctt
    24481 tcttttcttc ttgaattgag attttatttt tagacgcgac aatggtttga gcgtctgctt
    24541 tcctaggatg ttctggacgt ggcataatat ttcgccaacc atggtgcttc aacagttgat
    24601 agaaggcatc acgtgtgtag gaacgaccta actccttttt ataagcctga aataaggcat
    24661 caattgtaac aaattctcct gcctctgtag ccttcaaatg gcgggcaaga aagactttct
    24721 tttcctcaat cgtcatatat gcatggttac ggccaccacg tgtttcttga aggagagagt
    24781 cgagttcgaa ctcctcatat ttttttacat ttcgccaaat cgttgtttga ttacagtcta
    24841 tagtgtattg aatctataac agtacacctt agctgctaaa atatttctat aaattagttt
    24901 gactttcctg atagagttgt tcacatctta tttc
//
LOCUS       DAYYQN010000030        24603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYYQN010000030 DAYYQN010000000
VERSION     DAYYQN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.030"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(242..1339)
                     /locus_tag="ACHBRQ_001146"
     CDS             complement(242..1339)
                     /locus_tag="ACHBRQ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9592827.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(1349..2089)
                     /locus_tag="ACHBRQ_001147"
     CDS             complement(1349..2089)
                     /locus_tag="ACHBRQ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9592828.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYEILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2198..2551)
                     /gene="rsfS"
                     /locus_tag="ACHBRQ_001148"
     CDS             complement(2198..2551)
                     /gene="rsfS"
                     /locus_tag="ACHBRQ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9592829.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2564..3064)
                     /locus_tag="ACHBRQ_001149"
     CDS             complement(2564..3064)
                     /locus_tag="ACHBRQ_001149"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9592830.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(3065..3658)
                     /gene="yqeK"
                     /locus_tag="ACHBRQ_001150"
     CDS             complement(3065..3658)
                     /gene="yqeK"
                     /locus_tag="ACHBRQ_001150"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9592831.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(3658..4287)
                     /locus_tag="ACHBRQ_001151"
     CDS             complement(3658..4287)
                     /locus_tag="ACHBRQ_001151"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9592832.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4339..4650)
                     /gene="yhbY"
                     /locus_tag="ACHBRQ_001152"
     CDS             complement(4339..4650)
                     /gene="yhbY"
                     /locus_tag="ACHBRQ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9592833.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4865..5971)
                     /gene="yqeH"
                     /locus_tag="ACHBRQ_001153"
     CDS             complement(4865..5971)
                     /gene="yqeH"
                     /locus_tag="ACHBRQ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9592834.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(5974..6501)
                     /locus_tag="ACHBRQ_001154"
     CDS             complement(5974..6501)
                     /locus_tag="ACHBRQ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9592835.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            complement(6516..7424)
                     /locus_tag="ACHBRQ_001155"
     CDS             complement(6516..7424)
                     /locus_tag="ACHBRQ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9592836.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7481..8041)
                     /locus_tag="ACHBRQ_001156"
     CDS             complement(7481..8041)
                     /locus_tag="ACHBRQ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFM9592837.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8180..9388
                     /gene="sstT"
                     /locus_tag="ACHBRQ_001157"
                     /pseudo
     CDS             8180..9388
                     /gene="sstT"
                     /locus_tag="ACHBRQ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9456..10409
                     /locus_tag="ACHBRQ_001158"
     CDS             9456..10409
                     /locus_tag="ACHBRQ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9592838.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            10553..10933
                     /locus_tag="ACHBRQ_001159"
     CDS             10553..10933
                     /locus_tag="ACHBRQ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9592839.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            11032..11253
                     /locus_tag="ACHBRQ_001160"
     CDS             11032..11253
                     /locus_tag="ACHBRQ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HFM9592840.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            11270..11584
                     /gene="trxA"
                     /locus_tag="ACHBRQ_001161"
     CDS             11270..11584
                     /gene="trxA"
                     /locus_tag="ACHBRQ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9592841.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            11889..12422
                     /gene="queF"
                     /locus_tag="ACHBRQ_001162"
     CDS             11889..12422
                     /gene="queF"
                     /locus_tag="ACHBRQ_001162"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFM9592842.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQINREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            12484..12651
                     /locus_tag="ACHBRQ_001163"
     CDS             12484..12651
                     /locus_tag="ACHBRQ_001163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592843.1"
                     /translation="MFEVVDRTDEVSLKHCFEVADRTDEVSNHTYGKVKLTWFEEIFE
                     EYHTKKPCSSR"
     gene            complement(12676..13344)
                     /locus_tag="ACHBRQ_001164"
     CDS             complement(12676..13344)
                     /locus_tag="ACHBRQ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9592844.1"
                     /translation="MKKFVAELIGTFMLVFIGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAFSIGTVSGAHLNPAVSIAMFVNKRLSSKDLVNYILGQVVGAFIASGAVFLLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLIIGLS
                     LMAMILVGLNITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(13450..14240)
                     /locus_tag="ACHBRQ_001165"
                     /pseudo
     CDS             complement(13450..14240)
                     /locus_tag="ACHBRQ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            complement(14665..15429)
                     /locus_tag="ACHBRQ_001166"
     CDS             complement(14665..15429)
                     /locus_tag="ACHBRQ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592845.1"
                     /translation="MFKLSFQSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(15422..16192)
                     /locus_tag="ACHBRQ_001167"
     CDS             complement(15422..16192)
                     /locus_tag="ACHBRQ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592846.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
     gene            16439..17116
                     /locus_tag="ACHBRQ_001168"
     CDS             16439..17116
                     /locus_tag="ACHBRQ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9592847.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
     gene            complement(17176..18972)
                     /gene="pepF"
                     /locus_tag="ACHBRQ_001169"
     CDS             complement(17176..18972)
                     /gene="pepF"
                     /locus_tag="ACHBRQ_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9592848.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(18983..19726)
                     /locus_tag="ACHBRQ_001170"
     CDS             complement(18983..19726)
                     /locus_tag="ACHBRQ_001170"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9592849.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(19728..20678)
                     /gene="prmA"
                     /locus_tag="ACHBRQ_001171"
     CDS             complement(19728..20678)
                     /gene="prmA"
                     /locus_tag="ACHBRQ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9592850.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(20816..21289)
                     /locus_tag="ACHBRQ_001172"
     CDS             complement(20816..21289)
                     /locus_tag="ACHBRQ_001172"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592851.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            complement(21286..21714)
                     /locus_tag="ACHBRQ_001173"
     CDS             complement(21286..21714)
                     /locus_tag="ACHBRQ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9592852.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            complement(21695..22783)
                     /locus_tag="ACHBRQ_001174"
     CDS             complement(21695..22783)
                     /locus_tag="ACHBRQ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFM9592853.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLMLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(22802..23272)
                     /locus_tag="ACHBRQ_001175"
     CDS             complement(22802..23272)
                     /locus_tag="ACHBRQ_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9592854.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(23563..24015)
                     /locus_tag="ACHBRQ_001176"
     CDS             complement(23563..24015)
                     /locus_tag="ACHBRQ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM9592855.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(24052..24231)
                     /locus_tag="ACHBRQ_001177"
     CDS             complement(24052..24231)
                     /locus_tag="ACHBRQ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM9592856.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            complement(24368..>24603)
                     /locus_tag="ACHBRQ_001178"
     CDS             complement(24368..>24603)
                     /locus_tag="ACHBRQ_001178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592857.1"
                     /translation="IDVFEVVNRLLVITVENPDFEDLRVNQFVKIGDKKYRVRSVPMI
                     HSTPPQSVLDRDTFTIDYTDDELLDKEAVFTTH"
BASE COUNT         7163 a         5643 c         4569 g         7228 t
ORIGIN      
        1 atataaaaat ttatttgtct tttatattca atttatgatt tttaatataa ccagttctct
       61 tcattatacc acaaaataac aagcataaag cggattcaaa gataaagatt aaggttcata
      121 tactaatttt ctttattgac tttttgtctt ctgtattagg gagtttatca ttttccaaaa
      181 actttatctt caatttgtta tctaaaattt ctacaataaa aaagcctacc tttcagtaga
      241 cttagtttgt ttctattcta atcggcactc ttccaaaatt ttgctctgct atacttggat
      301 ttcctagttg gtaaatctgg tctgcttttt gggtcatagc atcccaaggt tctttgccaa
      361 ttcggctgac tagactgacc tgacctttca aagacttgag atgttgcctg cctttttcag
      421 taaatccaag gacatgaatg gcttctggca agacattttc tctagcctgc accaagatat
      481 aggacaagag gcgtctaaca cgcgccttgg tgtaacgttt ggtagtcact aactcgacca
      541 attcttccac agactgggct gttttaatag cttctttaat gcgcacagcc atttcttgat
      601 tgacctgata gatggtggtt aggtcgggat ttgacaagat ttgatagcgg agcaagggaa
      661 aatagtcttc ccagatcacc ttactggcct gctcaaatag ggcaacagaa ggcataaagc
      721 gttctaagaa atcttggtcc ttctgatgtt gacggagggc tgtcgccgag gcaaagtcca
      781 catctttatt cacagaatgg taacctgccc cctgacgctg aatcggatgc agcttgatat
      841 ttcgtcctgc aaccgccttg gcataggcca gagcaagaac atgattgggg gtattacctg
      901 aaaaatcaag accagcaaat tccttccaca tagcttgggt tttctgggga taggaaaggg
      961 aatcaggcag attttccaca aatttctcca tctcagcacc tttctctgtg tataagtcag
     1021 caattttctg ataatctcga acttcctctg tcccaaaaac tagactatca atgcccaagc
     1081 gatccaagat atccatagct ccttgaccaa agaaatcagc tgcctgaaca ctgactaaaa
     1141 agggcaattc cactaccaag tctgcgccat tttccagcgc catctgggtc cttgtccact
     1201 tatccacaat agcaggctct ccacgctgca tgaaattccc tgacatggca acaattttca
     1261 gtccctctgc ctgatccagc aggtatttat ggccattatg aaaaggattg aactccgcga
     1321 taatacctgt tatggtcatg ctaatctcct acttctgcgc cacaaaaaac caacgggtgc
     1381 tagtttctgt tggctcctta tcctcaaagt ctgcatagag tttgaaggac ttgaatccag
     1441 cctgttccag caagatctca taggtcaaga tctcataggt cctctcctca tgtacttcat
     1501 cgtggcgact aaaggaacca tcagcctcct tgataaagaa agtcagctca tggacgatgg
     1561 agtgaggaac ttcgtcctca tatgtatccc aaagcatggc aaaatcttcc gcattttcat
     1621 ggtaggaata gccagggaat acttcatctg tctggtaggt cgagtgcacg tcaaagatga
     1681 aaactccctc ttcattcagc gcattgtaca cgtccttaaa gacgtcccct acttccacct
     1741 catcctgcat atagcagata gagtccgaat aacacgtgac aaaatcgtat ttccctgcct
     1801 tggacaaatt cagcatatta ccttctataa aagcaatctt ttgcttggct gaagttgctc
     1861 tcttctccgc aatcttcaac atatccgcac tcaagtcaag tccagtcaca tcaaaaccag
     1921 cctgagagaa gcgcactgat tgaattcctg tcccacaagc caattccaag agtttctttc
     1981 tctccttggt cttaggcaaa tgacgcagag aaaaattcgt ccatttgtcg tataaactat
     2041 cgtccatgac cgcatcatag accgccgcaa aggtttcata agtcgccata atcatgatac
     2101 taagagctcc atggtaaaca ccactagccc tttacctttc tatcaatttt tctaaaataa
     2161 gcaaagaaac ccagttgact acaactgagt tcatcttcta agcaagagct tctgaaatat
     2221 ctactgaatt cgcctcatgc catagcttct ctagattata atgggcacgc atttcttctg
     2281 aaaagatatg cacgacaaca gcaccgaggt ccagtaagac ccagcctcca gctgcatcgc
     2341 cttcgacatg gctaccttta aagcctgctt gagctacttt ttcacggata ttagctgcga
     2401 tagcgtccaa ctgacggcta ttcattgagc tagtgatgac aaagtagtcc gtcacactag
     2461 tcaaatcttg tacatcaagt gcgaggatat cctccgcacg tttctcatca gccgctttca
     2521 cgactagttc tagtaattct ttttcgttca tttaatcctc tttttagaaa atatgtttgt
     2581 aatctagaac atctgcaaag cgctcctccc aaatgtcctc ataaaattca ttaatcgttt
     2641 ctgctggaat gtcatcccca tcaaaggttg tgacagcacc ttctggaaca ataagctgat
     2701 agccatattc aaaggcaacc ttgacagagg tatccacaca atattctgtc tgcataccac
     2761 ataaaactaa tttttcaatc ccctgcttat ccaagtattc ttttaagcca gtttctttga
     2821 aaatactgtt atatttcttc tgaaagacct tttcagcagg ttttcgattt aaaagcgcag
     2881 ataactgcca atcttctgat gtttgagctt cagagttctc aatatgttga acatagataa
     2941 tttcaatatt cttgcttcta gcctggtttt gtaaataaga aattttttcc aatagacttt
     3001 ttgtctggaa cccagtttct actaaaacat tctgaacatc aatgattata aaagccatct
     3061 tcatttagtt ctctttcaaa tagtgcacaa aggcgttata ggtttcaagg gtttggggat
     3121 agatggggaa tccctgatga gctagatact ccacggtacg agctgtttcg taggccaccg
     3181 ccttattgag cgatagactt gcaatctcac gcgccacatc tactccagga aaggctcgat
     3241 tgtgctcgat atagtctgct acatagacaa ccttatctag gtctgtcatc tgaccagcac
     3301 cgactgtatg gacttcaatg gctcgcagga tttctgaatc atgcaaatcc aaatcttcct
     3361 gaattttgta aattccgacc ataccatgcc agacattatt accccagttt ttgaggtcag
     3421 ggtctagctg gtaacggtca atcaaagcta gaaattcctg atctgacaac tttttagcat
     3481 agtcatgaag aagacctgct agacttgctt tctcaacatc tacaccaaag cgctgagcta
     3541 attccatagc ggcacgctcc acacctagac aatgggttag acgtttctca ggtagaagct
     3601 ctgccatttt ttccaacaag gcctcacggg agcagttgat atagtcttga taggacatca
     3661 gtagagcccc tccttctcga tgtagtctag cactggctga ggtaggagaa agttgggttt
     3721 ccgaccttgg gcaatgaagt cccgcaccat gctggacgag atatccatga gcggtacgtc
     3781 cacccagata actggatagg aagtccctgc cttgtagcgt ggacgctgaa cccccacaaa
     3841 ctgaaccatg tcaaccagtt catcaattcg gtaccactta ggcagatagt caaccatgtc
     3901 ggcaccgatg ataaagtaat aatccgtatc tggattcttc tctgtcaaaa tcttcatggt
     3961 gtcgtaggtg taggaaatac ccttgcgctc caactcaatg gtttcaatga ctaggccgtc
     4021 aattccctca attgccaact caagcatctt gagacgatgg tgttcaggga tggtttcctt
     4081 tttatcaacg tgaggaggtt ggtattcagg catgagaaga acttgatcca gtcccaactg
     4141 ttgccgtact tgatccgcaa caatgagatg ggcattgtga acagggttaa aattcccccc
     4201 taaaatccca acttgtttgc gttttttctc cttgatttct ggctccaact ctaccttggt
     4261 aaagggagtc aatagttcga ttgtcatagg ctagtcccct ttatttctct ataaaaacag
     4321 ttatttggag tttcggtctt agatttcttt gactttctta gaaatcttgc gattttcttt
     4381 cttgctagat tgtttaaaca aaatcaagat gcgtcctatt ttttggactg tatccacacc
     4441 gatttcttct tccaaaattt cagctacttc gtggatgttt tcatctgtgt tttgtaagag
     4501 agtaaccttg attaattcac gcgcatcaag agcttgacgg acgctggttt tgatttggtc
     4561 gttgagtcca tttttcccga tttggatgat aggtttgagg gtgtgtgcct ggctgttgag
     4621 gaaggcacgt tgttttgatg ttaatgacat aatttcttta aaagagtttc tttttatact
     4681 tttctgaagt ggtgacggac gtcagcaaag tccttcggac tttcatgact aaaatttgag
     4741 cctaaggtct caaattttcc gcagttggta caatcacttg taccaactta caccacagcg
     4801 aaaagtattc tctatggggc tcgccttgct cgcccaaatt cagacaaccc tttcctttct
     4861 gtgcttaaat aattgctttt cgtgtgacga cggcgacgcc ttctggtgcc cagacggcga
     4921 cttttgctat gcctgttaca cgaatccaac ctaggcctga gatgactagg tctgtcttat
     4981 ctttaatggt aaagacatgc tggactagcc ttgggaaatc ttctttttcc ttgctatttg
     5041 gtggtgtcag aagagttccc aggtgcttat cgtagaaagc actagctcct tcaagcttgc
     5101 tacgatggag tttgagttca ttatcaaaga aagcagtaaa tccttgcttt tctcctgcta
     5161 taaagtcaaa gcgtcccaaa ccacctaaaa atagggtttg ctcaggatta agctgatagg
     5221 tcttaggctt gatttccttt ttaggactga catacttgag gtttttggcc gtcaagtagt
     5281 gagccatctg gtggcggtgg ataattcccg gcgtatcgta aatataagat ccgtcgtcaa
     5341 gcggaatctc tattttgtcc aaggttgtcc ctgggaagcg tgaagtagtg atgacattct
     5401 gatcacccgt gatttcttgg ataatagcat tgattagagt tgattttcca acgttggtca
     5461 caccgaccac atagacatcg cggcccttac ggtagtgttc aatcttgtca atgacttcct
     5521 taatggcatg tttattttgt gctgaagtta ggaccacatc gactggacga agaccttctt
     5581 catgggcacg tttcatgagc cactggctaa tcttaccaga cttaactgac ttagggagga
     5641 tatctttttt atttcctacc aagaggacat cattgcccga gacgaaacgt ggtaaacctg
     5701 ggatgacaga gccattaaaa tcaaagatat caatgacatt gaccactaag gcatcactgt
     5761 ctcccacctc gtgcaagagc ttgaggaaat catcgttcgt caactggaca tctgtgattt
     5821 cattgtagtg gcggagacgg aaacagcgtt ggcaatagac ttcgccagtc tccaaacctt
     5881 tttcaagtgc cgactggggg gtaaaaccaa gaccagcttt atctgtcgtc tgaatggttg
     5941 ctccacaacc aatacagaga atttcttcca tagttaaatt ccttttttat atgtaatcgg
     6001 tccgtacttt tcagtgattt ttctcataac acgacgctca cgagttcggt taatctgcgt
     6061 tttgattgag tcatgttgga ccaagggttt gactaaaatt gaccgaatcc ctgcacggtg
     6121 ggctgctcgt atatctgtca tgagctggtc gccaaccatg accacttcct ttttgtcata
     6181 gtggaattcc ttcatggcac ggtcaatacc aaatgtgaag ggcttcaagg cccagtaaac
     6241 gtaatcaatc ccaaatttct caactgctcg ttgaacgcgt tttttggtgt tatttgacac
     6301 tacgataatg ccaatacccg cgtcccgaag gtcatgtagc cattgcttca tctctggcgt
     6361 tccatcaggg ttgttccaag caatgagggt attatccaaa tcgaccaaaa cagccttgat
     6421 tccctgcgcc tgcaggcttg ggactgtcag atcatagact gcttccacag caaaatctgg
     6481 tatataattt tcaatcgcca ttctggctcc ttttcttaac tttttttcgc aattttcctt
     6541 agtaacaagg ataaaacaat ccacaaacct gcactagcca aactgatata gagccaagca
     6601 tggggctcat ctgttaaagg taagggaaca ttcattccga aaaagcctgt cacgactgcc
     6661 aaaacagcta gcaagactga aatgatagtc aaggttgtca aattgtcatt cagattattg
     6721 tttagaatat tgttgtaaga ggctgaaagc tgctgtaaaa tctgagagat taggtctgtc
     6781 atggatacca gctgatgagc ctcaatcata gcatcatcaa actgttctct ctcaatctca
     6841 tcaaaactac gatacaaggc atgaccttga atatgctcta acaaaatccg attttgtttg
     6901 gcagctgccg tcagataaac cataccagtc tccaaatcag acagggcaaa gaggtttttc
     6961 ttggtagttc gctggcgcaa gaggtcattg acctcatccc tactcttgtc catctgctca
     7021 atgacaggat agtaggcatt gctgatgatt tccagactgg caaagagaaa cttataaatc
     7081 gaaagcgtgt catggctctc cagataacga gtcatctgtt caatgacata ggcgttcttg
     7141 gtattactaa tggtaatcag gcgacgatgc tcgacgataa aggtcatggg aaaagtctca
     7201 tagtaggcct tgtccttttt tacgtctagg acattataga taaaggtaac cgtcccactt
     7261 tcacggtggt agtccatgtg ggcgcgctcg tttctatcca gtgcgtattc aatggtttcc
     7321 ttgtccaaac cgtaaatttc agaaaggtct tctagtttat tcaactttcc taggtctagg
     7381 tctatccagg tacaaccatt gcccaactgc ttttctaaaa ccatcttttc tccttattaa
     7441 actccctcta ttgtaccaca aattacttaa aataacaggt ctagtctgtg cgtgagaaat
     7501 tgctaacaac tgtgatattt cggttaggga caaagtgaag ggcttgctcc tcgctacgca
     7561 gctgtgtcgt acgaattccc acactgacaa ccttacccga tacagtaatc ggtccatttg
     7621 tcagaacgac ctcatctccc acatccagtt gacgttcaaa gaggatgaaa aagccattga
     7681 tgacatcaga cagaaagcct tgggctccca taccaatcgc taccccagca ataccagctc
     7741 cagccagcaa actagaaact ggcaaaccta aaatagacaa aatgcagtag agtaaaaaga
     7801 aatatagcgt ataattaaac acattttcta gtaaacgtga gatggttttt tggcgtccga
     7861 catcatgacg agacattttt agagaaggtt tgacaattct ctgcaccatg gtatgaagca
     7921 tttttttagc aatataaaat acaatcaaaa gcagtaaaag agaaatgacc ttggttagaa
     7981 tattctcgat aatggttgtc acatctagct tttcaatata agcctgaata aatttttgca
     8041 tataaaactc ctttatttca ttataccata ttttcatcag tctcattctc cataaatatt
     8101 caaaaaatag acagaaaatt cttgattttg tctatcattt atactataat cataatcact
     8161 acacaaaaag gagattatta tgaaaagaat cattcatgct tggaataagg caagccttgt
     8221 caagcgtatc ttgattggta tgctttgtgg ggaatcctag gactgaccct tcctaatctc
     8281 tcagggattg ggctactcgg ggatttattc gttggaggtc taaaagctgt tgctcctatc
     8341 ctagtctttg ccctcgttgc caatgccctt tcccaacatc aaaagggaca agatagcaat
     8401 atgaaaactg ttgttttctt gtatatccta tgatagggac tttcgccgct gctcttgtag
     8461 ctgtactagc aagtttcatc gtccctattg aaattaccct aaatagtgcc aatactgaaa
     8521 ttgcaccacc agatggggtt gggcaggttc tcagcaacct cttgctcaaa ctggttgaca
     8581 gcccagtcaa cgccctgttt actgctaact atattggaat cttatcttgg gcagtcattt
     8641 ttggaatcgc tatgagagaa gccagtaaaa atagtaaaga attgctaaaa actatcgctg
     8701 acgtgacttc taaaattgtc gaatggatca tcaatctggc tccatttgga agccttggtc
     8761 ttgtttttaa aaccatttct gacaagggag tcggaagcct tgccaactac ggtattttat
     8821 tggttctatt agtaacgact atgctttttg ttgcccttgt ggtcaccctt tgattgcctt
     8881 cttctttatg agacgtaatc cttaccctct agtttggaac tgcctccgtg tcagcggtgt
     8941 gacagccttt ttcactcgta gttctgcggc taacattcct gtcaacatga aactctgcca
     9001 tgaccttgga ctcaacccag atacctattc tgtttctatc ccactcggtt ctactatcaa
     9061 tatggctgga gtagcgatta ccattaacgt tttgaccctt gctgcagtta acactcttgg
     9121 aattcctgtt gactttgcca cagcctttgt cctcagtgtg gtagcagcta tctcagcctg
     9181 tggtgcttca ggtattgccg gaggttccct ccttcttatc ccagttgctt gtagcctttt
     9241 cggtatttct aacgatattg ccatacaagt tgttggggtt ggttttgtga ttggtgtcat
     9301 ccaagactca tgtgaaacag cccttaactc ttctacagat gtcctcttta ccgccgttgc
     9361 cgaatacgca gcaacccgta aaaaataact catcaaggca agcctgctca tgtcttgtct
     9421 tttacgcttt tattctaact tattaggaaa ttcttatgtc tattagccaa cgtacgacca
     9481 agctcatctt agctacctgt cttgcctgcc tgcttgctta ttttctcaat ctttcatcag
     9541 cagtttcggc tggaattatc gctctcttga gcctatctga tacgcgtaga agtactttaa
     9601 aactggctcg caatcgtctt ttttctatgc ttctagctct ggctatcggt gttctagctt
     9661 ttcacttgag cggatttcat atctggagtc tcggcctcta tctggccttc tacgttcctt
     9721 tagcctacaa gatgggctgg gaaattggca tcacaccaag cactgttttg gttagccatc
     9781 tcttggttca agagtcaacc tctccagacc ttctagtcaa tgaattcctt ctctttgcta
     9841 ttggtacagg atttgccttg cttgttaatc tctatatgcc ttcacgagaa gaggaaatcc
     9901 agcactacca cacgctggtg gaagaaaagt taaaagatat cctccagcgc ttcaaatact
     9961 atttatccag aggagacgga cgcaaccgag cacagctggt agcagaatta gacacgcttt
    10021 tgaaagaagc cctcagactg gtctatttgg atcactctga ccacctcttt caccagaccg
    10081 actaccatat ccactacttt gagatgagac aacgacaaag tcgtatcctg agaaacatgg
    10141 cccaacagat taacacttgt caccttgccg ccagtgaaag cctgatctta gcgcaactct
    10201 tttcaaaaat tgcaggtcaa ctgagccaga ccaatcctgc ttctgatttg ctagatgaaa
    10261 ttgaacgtta tctggaagtc ttccggaacc gcagtctgcc caagacaaga gaagagtttg
    10321 aaacccgcgc cacccttctt caactcctac gtgaagccaa aaccttcatc caagtaaaag
    10381 ttgattttta ccaaaaatat ggacagtaaa aaataaaacc actaaaaaac agagcttgcc
    10441 agcattcttg aaaagtagta taattattgc atgcgcaatc atcttgtgac gcagagactg
    10501 cccgttaatg gactttcttc tattttatga ctctaaaaag aaaggagaca ctatgaccta
    10561 tttggaaaaa tggtttgact tcaatcggcg tcagaaagaa attgaaagtc tcttggaaga
    10621 gaccattgcc caacagagcg agcaaagtct gaccttgaaa gagttctacc tgctctacta
    10681 tctggacttg gctgaagaaa aatctctacg ccagattgac ctgccagata agcttcatct
    10741 gagcccgagc gcagtttcta ggatggtggc tcgcttggaa gctaaaaatt gcggtctact
    10801 cagtcgcatg tgttgccatc aagatagacg ctctagtttt atctgcctga caaatgatgg
    10861 gcaaaagaca ctggcctcgc tacaaaagac tgtcgaagaa agcttggaag caggtttgga
    10921 tttcttgatt taagatgatt ttaaaaaaag ttgcgtgcgc aatcattttt cttgacattc
    10981 tcttttataa ggagtaaaat aaagttagca ttaaacaaag gagttttaaa tatgattgaa
    11041 attacctata tagatgccag caagaacgaa agaactgtga ctttcgagtc ttatgaagac
    11101 tttgaacgtt cgcaacaagc ttgccttatc ggcgtcgcag actactaccc tgtccaaaaa
    11161 ttaacttaca agggccataa tttggactac catgggactt atggagatat cttcttctat
    11221 ctcatgaaac aagatttaag ccaatataac taaaaaaagg agaaatacaa tggcaaaagc
    11281 aattacagat gcaacattcg aacaagaaac aaaagacggt ttggtcttag tagacttctg
    11341 ggcaacttgg tgtggtccat gtcgtatgca aggtccaatc ttggacaaat tgtctgaaga
    11401 actttcagaa gatgtcttga aaatcgttaa aatggacgtt gatgaaaatc caaacacagc
    11461 tcgtgctttt ggaatcatgt ctattccaac tcttctcttc aaaaaagacg gccaagttgt
    11521 caaacaggtt gcaggtgttc acacagcaga acaaatcaag gccatcattg ctgaattgag
    11581 ctaatcaaac gagagaccaa gtactttctt tggtctcttt tttcttgccc tttgccattt
    11641 ttcaaaaaat atgctagact atagcagatt gaaactagaa tagtacacct ctacttctaa
    11701 aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctt atttcatttt
    11761 actatataag tagaatatta cgatgtttgg gatatgccat cgctaaaaaa actcgatttg
    11821 gtatttttta gctccctaat gggagctttt tgcatgctct gaacatttcc cattttggaa
    11881 ggagtactat gaaacgtcaa tcagccttgg tcgtctttag tggtggtcaa gattcaacaa
    11941 cctgcctttt ctgggccatg caacactatg aaacagtcga agctgtcacc tttgcctacg
    12001 gccaacgtca tcacctcgaa attcaaatta atagagaaat cgctaaggaa cagggcattc
    12061 gtcaccatat cctcgatatg tctctgctgg gacaaatcac tgctcagcca gactttgcga
    12121 cgattcatat ttcctacatt cctgacaagc tctgtgtcga gtcaaaatcc ctcaaactat
    12181 atctatttag ctaccgaaac cacggagatt tccacgaaaa ctgtatcaac accatcggga
    12241 aagacttggt caacttgcta gaccctcgct atttagaagt ctgggggaaa ttcactccgc
    12301 gcggtggcat ttcaatcgac ccctactaca actacggtaa gcaaggaact aagtatgagg
    12361 gcttggcaga acaacgcctc ttccaacacg acctttatcc agagaaaatt gacaaccgct
    12421 aaagtcatac tcaatgaaaa tcaaagagca aactaggaag ttagccgcag gttactcaaa
    12481 gcagtgtttg aggttgtaga tagaactgac gaagtcagct taaaacactg ttttgaggtt
    12541 gcagatagaa ctgacgaagt cagtaaccat acctacggca aggtgaagct gacgtggttt
    12601 gaagagattt tcgaagagta tcatacgaaa aagccctgtt cctcaagatg aggagcaagg
    12661 ctttttgagt ttcaattatt cttctgttcc aaggaaattt tttgcaacaa gggctgcaag
    12721 aactccacca gcgattggtg caaggatgaa aatccaaact tgttgaaggg ctgcgccgcc
    12781 taccaagaca gctggtgcca agctacgagc tgggtttact gaaagtccag taatgttcaa
    12841 tccgacaagg atcatcgcca tcaaggacaa accgattatc aaaccagcaa tcgcgccatt
    12901 tcctttactt tctgaagtca cagtcatgat aaccaaaaca aacaagaaag ttgcgatgac
    12961 ttcaaacaag aaaccaccaa agacagtgac accgtttgcc aaagcatttt caccaagact
    13021 agcagttgac atacctgaat ttgccaagag caagaagaca gcgccagaag caataaaagc
    13081 tccgacaact tgtccaagga tgtagtttac aaggtctttt gatgacaaac gcttgttaac
    13141 aaacatagca atcgaaacag ctgggttcaa gtgagcacct gaaacagttc caattgagaa
    13201 agctgcgact acgattgcca aaccaaaggc aaaagcaatt ccaaggtgac caaggccatc
    13261 aaaaccattt ccaaaaacaa cagctcctgt tccgatgaac acaagcatga acgtaccgat
    13321 taactcagca acaaattttt tcattttctt tctccttttt tcaaaaacta gatactagtc
    13381 tatcaaaaga gggaaagggt ttcaagaaaa ttgcttggaa agttttttga aaatctgact
    13441 cgctttttct tagtattgaa aagactggat agcttctttc aagtcatctt gtaaactatt
    13501 tctctggtca agttgaacat agacttccaa cagacaggat ctgaagttgg aaaatttata
    13561 aaaatcttct ctttcttcta tcggaaagtc aacagctttt atccaagaag ctacttgctc
    13621 ttgcgctaac ttcccttgta aaataggttc atagatcact cttgctaaac gccaatcctc
    13681 atcatctgta aagcgaatcg aaactctttt aaatagttgg ccaagtatat caaatacttc
    13741 atgaactctg tttttaggaa agttgggatg acaaaccacc tctgtcagta aatcggctcc
    13801 atgtgcaaaa gcatgaaccc aaccatactg acttgaaaaa cccgttgtat ccttttcttt
    13861 tgaaagataa tacaaacctt gatttaaaag gacattacga attcctgcat ttaattcctg
    13921 ataaaaatcg attgctggtt ggcatctgca gacaagagat ttgcataaat aagcgcccta
    13981 aaagaacgtt caagggttga caggcctacc ttatcaatat ctttatctaa ccctccatca
    14041 gatgaaatcc cttcagcaat gaaatgaaat tgttcctgtg taaatagctc ttcctgaatc
    14101 cctctagcaa agcttgtaaa aacaaggtca tcacgaattt ctggagaagg atctcccaaa
    14161 tgatcaagta gccactggat ttcttcctgg ttataacttg gtttttcttc tgctattttt
    14221 ctaagtaact tttgatacat ggtcaatacc tctacatttc tagcaactgt tcaaaaagct
    14281 gtccttaaat gactttagca atttgtaaaa ttaagttaaa gagaaaaaga catttatatt
    14341 cagagaaaca gcacatttct gaaaggatac ataaatgatt tattccatat taccacaaat
    14401 gaacttgtaa tgatagaact ttatcacaaa aatcacattt ctgtctcaaa aattgcgaaa
    14461 aactagttat aataaagtga aataaaaact atacaaattg attagggaat ttaaaacaat
    14521 ttctaacaat attttaaaaa ctgaaccgta ctatttcaac ttccataaat tacaatattg
    14581 ataggcacag ttcccatacc tgtccaaatt cgaataacgt ttagtataaa ttagaatatg
    14641 actacaacat cacaaataat tagactatct ataatatcta cgatgtaaca tatacatagg
    14701 aagcaccgtt atcaagagcg ccaaggctat caaccagata agatgaagat acaaacctgt
    14761 cacctcacct ccccatataa tacgaatcat gcgggtagcg tgatacaagg gagaaaacca
    14821 agtcaaatca ccaagtattg ggggcaagga agtaatggga aagaaggtat ctgaaaacat
    14881 ataaagagga cggattacca aaacattgta gtaattcaga aattccctag acggtgccaa
    14941 gtaggtaaag attagtccta aaacaccaaa caaataaccc actataaata aaatcagagg
    15001 aatcaataac atcaagacat gaaaatgcgc tgtgataagc atggaaatca gccaaaaaat
    15061 cccaccgaaa atcactgcac gaatacctgc ccagatagcc tgacccacca caatatcact
    15121 cgtattgatt ggcgtcatca acatagcatc ataagtcttt tcaatcttat actggataaa
    15181 ggcattgacc gttgtttctg cggtggccgc agacatggca gtcgcagcaa ttaaacctgt
    15241 aatgacaaaa tcacggtaga gcataccgtt aacatgcgtc aagtaggcac caactccaaa
    15301 tcccattgct aaaagataaa agagggggtc gataaggttg gggacaattg aaattttaaa
    15361 taaatttcta aaactcatgt agttgcgtac cacaacgtgt ccgatggact gaaatgataa
    15421 cttaaacatg caagtcctcc ttctctgtcc cagtcaaccc caaaaaaagc gtctcaaggt
    15481 tgtcagcatc atacttgatg agcaagtctt ctacagttcc ttcttctttg atagtccctt
    15541 ggtggagcat cagcacccta tctgtcaaat attcaatctc attcatatag tgggaagtaa
    15601 taatgataga aacacctaat tccttcaact ccaaaagttt atcccaaata atattgcggg
    15661 attgaatgtc taaaccaact gtcggttcat ctaaaatgat aatatcaggc cgattgatga
    15721 gagctcttgc caagacaaga cgtcgtcgca tcccacccga cagctgattc accatctttt
    15781 gcgcatgttt gctcagttga acaaaatcca agagagaatc gattctcttc ctaagttcga
    15841 cagatgagat accataacat agaccgtgag caaaaagatt atcataaaca ttcaaggtca
    15901 tatccagcat atcctcctgc cctacaatac ccagacaaga tttactctta cgacgattgg
    15961 tttcaatatt ttgccccaag atatcaatct ctcctgagga aattgaggtc agtgagtaaa
    16021 tcatctttaa agtggatgtt ttgccagcac cgttgattcc caagataccc aaacactctc
    16081 cttctcccaa ttcaaagcta attcccttga cagcttcata agaaccatat tgctttttta
    16141 gatttttaac acttaacttc ttcactcttt ctttccccca caaatagagc atgttcgata
    16201 ctttgttaat tcatctcact tttgaaaaaa tgttgagaat gtaatcttaa gattaacgtc
    16261 cttcaactgt ggatactgct gcaattactt ttgtaatttt acctccattt aaactcagac
    16321 aataaaattt aaaatattct ctagtaaaac atttttaaaa tctatttttc ttgtgcttta
    16381 attatagtat aataaaagat agaaaataat cgatatatga aatttggaga aaatttttat
    16441 ggaaccagat attttcatca tcacaaaact cttatcagac ccttctcgga tgcacatttt
    16501 agatattcta atggatggaa aagctcacac ggttaacgaa attgccagct ttaccaaaat
    16561 caagcaacat actgtcagct atcacctcaa attacttacc gaagcgcagg taaccactct
    16621 acaaacctat ggacgttttc attattattc tttaaaaagt gctgccattg cagaaatgct
    16681 ggaatttctc agcttctact ctcctcagcg tgatgtcaaa agttataaac aacacattca
    16741 caaaaaggaa ttaaaggtcg ctcgcacctg ctatgaccat attgctggtg agcttggcat
    16801 ttccattacc aattatctct tgcaagaaaa tctcctcagc gaatcagaaa aagacttcca
    16861 actcactgaa gctgggaagt cttatttcca aagaaaatta cacattgata cggatgaatt
    16921 gaaaaagaaa aaacggaaat tttgccctaa atgtttggat tggagcgaac gtaaaaatca
    16981 tgtcggcgga gctctcggca atgctctttt agaatttttc agtgaaaaac aattggtcat
    17041 ccctgcacaa acgccacgtt cacttaccat tacagagaag ggcaaagaat tcctcgaaaa
    17101 ggaatggggg atttaaacat ggacttttaa gcaaataact gtccataccc attctataac
    17161 aacctctcta tccccttact cccccaactc agcactgtag gcgataatct ggtcaactgt
    17221 gtcagacaag aattggatgg tatcacggag tggtttgtct gttgaaatat cagcaccgat
    17281 aatcatggct gactcaagtg gtgtcttgct accacctgat ttgaggagat taagccagtc
    17341 ttcagctcca gtttctgagt ttttaagatg aaggtaacca gctgtcgaga tgactagtcc
    17401 tgctgagtag gtgtaactat acaagcccat atagtagtga gcttggcgca tccaagtcaa
    17461 agctgcatca tcatcaattt caatagcatc tccccagaaa tctgtcaaaa cttccttcat
    17521 aatgctgttg agtttgcttg ctccaaaggt ctccccttct tcaatcaatg tatacacctt
    17581 acgctggaag gctgcttcca agaggtgggt gataaagtta tggaagtagg tgtctgtcaa
    17641 gcgatgagcc agagcgaagc gtttttgacg tgggtcatta gactggttct ccaagtaatc
    17701 actgagtagc aattcattga aggttgacgg tgcttcaaca tagtaggtcg acatatgggc
    17761 attgaagtaa ctttgatgat tgtctgaaaa gatgaattga ccagaatgcc cgatttcatg
    17821 aatcaaggta tagacatcgc tcaaacggcc tgtccagctc atgagtacat aagggtgtac
    17881 gcgatatggg tccgccgcat aaccaccgga atccttgcca ctgttagcag caaagtccac
    17941 ccagcgctct tcttggtaac gagcaacttc ctgacaatat tcttgcccca aaggttctac
    18001 cgacttcatg accaaatcat aggcatcgtc aatagtcact tcaggattca gggcgctgtc
    18061 caagtccaat ttccagtctg caaaggtcat cttttcaaga ccatttacct tggcaacatg
    18121 cttgaggtat ctctgagcga ctggtgcaaa gtccttcatg atgaggtcaa tctggcgatc
    18181 aaacatgaca cggtccactt cttgttcagc tagaagataa tcaaagacag agtcgtatcc
    18241 cttcatatca gccaagagtt tttcagactt gacctgagcc agataggctg ctgcagccgt
    18301 attttggtgc ttacgaagtc cctctgagaa ggaacggaag gatttctcac gaacctcggc
    18361 atcctcgtga ttttggtaga aattctcata ggtgacaaag ctgtttttgt aggtcttgcc
    18421 atgggcttca aagtcagcca tttcaaaatc cccagctcgc atcttagtat aaatgtcctg
    18481 cggactatag aaaacttcac cgagattggt caaggccttc tccacatcag cccctagata
    18541 gtgggctttt ttgattttgg cctgacgaat agctgccgtc aagtgaggca attcacccaa
    18601 acggtccaag acttcctcgt ctgctaccac caaggcatcg tcaaagaagg tcaaggctac
    18661 gctggcatct gtttcaaatt ccatcccagc ttgggcaata ttggcaaatt cgtcattgct
    18721 atagtccgtc gtctgaggca taaaaccata gttgccaata tggctcatct gaatgtagat
    18781 ctgttccaat tccgcaaagg ccttctcgaa atcctcaaaa gtgtgaagat tgcccttgta
    18841 atcacggcta aactggttga tgtcttcgcg agctttctcg attgcacgca agaaatcctc
    18901 acggtcttgg tatagggctg ttaagtccca gagttccttc tctggaaatt ctgaacggtg
    18961 tttttgttcc attttcttcc tcttatttct ctaattctac taaaacacta agggctgata
    19021 aggcgtaaag cggtgctgtt tctgctcgca aaatacgagg acctaggcct accaaaacgg
    19081 ctcccttagc ttcaaaactt tcgatttctg caggtgagag accgccttct ggtccaaaga
    19141 taaagagcag tttggctcct gtttcaagac cagagaccgc ttgtaagagc gcagcggctt
    19201 ctccttcttt agctgattct tcataggcta ctatgataga gtcaaactgg tccagctgag
    19261 ctagaaaatc tgcttttttc tcgaaaagtt taatacttgg tacaatatta cgcttgcttt
    19321 gctcggctgc tccaagggca attttttcta gtttttcaac ttttttaccc aatttcttgc
    19381 catcccactt ggcaactgac cagtctgcag gaaaggccca gatttggcta gcccccagtt
    19441 cggttacttt ttgagcgatg aactccagct tgtctccctt gggaaatcca gatgcgatgg
    19501 tcacttggac tggtagttcc acattgtcat ttaattcttg gaccaactca aactgacgat
    19561 tttccatatc cagcacgcgc gccaagcgct tgatgccatc atcaaagact aaggtaacct
    19621 catcctcttc tttcaagcgc ataacctgaa acatatgctt actggtttcc ttgtcctcga
    19681 tagtgacagg agagatagca ctgcctttta caaaatactg ctgcatgcta gcctccaatc
    19741 acaccagaga tatccttggt tttcttaaag acacaggtat tccattcccc ttgaaccatg
    19801 tgagtttcga ggaaaaatcc agctgattca gccgactcgc gcaccatgtc ccacttatcc
    19861 ttgataatgc cactcatgat caggtagcct tcgtccttga ccaaacgata ggcatcgtct
    19921 attagatgaa tgaggatatc cgccaagata ttagccacga tgacatctgc ttcaatctca
    19981 actcccttaa gcaagtctcc tgctgctaca tggatatttt ccataccagt gttgagctca
    20041 atattttcct gagcgacacg aaccgccaca tcatccaagt cataggcgaa aatctccttg
    20101 gcacctagga gtgagcttgc aatagaaaga acccctgaac cagtccctac atctagcact
    20161 gtttcgccac cacgaagaac ctgttccaag gcaaagaggc tcatcttggt tgttgggtgg
    20221 gtccctgtcc caaaggccat accaggatcc agcttgataa tcttttctcc tgctgttgcc
    20281 tcatagtccg tccatgacgg tacgatggtc aggtcatggg tgatgcgtgc cggttcataa
    20341 tacttcttcc agttgtcagc ccagtcttcc tcagccaagg cagtcgtgcc catcttgact
    20401 tctcctaggt ccataaagtc ggtcaattct gccagacgag cctgcaaatc cgcttcaact
    20461 gctgctacat caaccgtttc agggtagtag gcggtcacta cgatttcttc ctgttgctcc
    20521 acctctggga aaatttcacc gaatcggtcc acatttccca catagtccat gctgtcttca
    20581 attgctaccc cttgggcacc taactcaatc aagagattgg aaaccaactc ctctccctca
    20641 cgcttcactg taacttttaa ctcttgccat gtttccatta ttaagatacc aagcccgtaa
    20701 aacacaaagc caaaatagga aactctctga agacgtttgt gtctaagaga agtttatctt
    20761 tttggcaccg tgtttagggc gggttcagtt tagaaattta accgaaccat cctttctatt
    20821 tctctatcaa ttttttcatg tagaccatat ccatgccttc tttttcagcc tcaatgtgga
    20881 tttgacgata gccgtttttc tcgtaaaaag caaccatgcc tgcatcctgt aacaccgtac
    20941 acaaatccca ttgtgtgatg gttggaaatt ctttttccag taagcttaaa cctttagatc
    21001 catacccttt tccttgatac tgaggcaaaa tcgctgccgt tcccaaccaa gctttcgtta
    21061 actcggcatt ggtctgaact cgtaaaaatc cgatgttttc ttcatcttct tttacaaagt
    21121 agtaatagct attgggacgc tcgactaatt tccacttaat tcgttcgcag tcctcgagat
    21181 aagggtcata ttcatcatgg tatttttcat aaacagcctt aaagctggct ctctgaattg
    21241 caataatagt ttctaaatcc tctgctcccg ctcgaacaag tgttatcata aactttcctc
    21301 caaataatct ctcaatcttc cctgcaactg aggcactgcc tgtttagaat tgagaaattc
    21361 ctcaataggc atcaatttat agccctgtcc ttcatctccg aagatgatat tgtcaaattg
    21421 ttcttgtctt agctgaccaa ccataaagac cgatttcttg cctttaaaaa ttacgctagg
    21481 ataaatcttg ctccaaagca gacagtcttc atctaaatga attcccagtt cctcataaac
    21541 ttcacgccga gcgcattcaa aagggctttc gtccccttca cggccaccac ctggcaactc
    21601 ccacatgttg ggacaaggga tatcgtcctt gtcatcacgc aaaatcgtca gtagcttatc
    21661 cccacaaaag agggcaatct tgcaaccttg gaaatcagaa atttctagtt ccatcttcag
    21721 ttccttctaa catttccttt tccagctctg ctaacaagtt ttcataatag cgtttctcgt
    21781 ccctcaacat tcgactacta tccattttct gtctagcaat cttgagagcc ttacgagttc
    21841 gatctacatc tttctttacc ttaaattgat accaagcttg tatcacttga agattggaca
    21901 gtttgagaga cagaaacgat ttgacctgtc gaatactagc atattgctcc gcttgctcaa
    21961 aatctccttc caacaaggcg atatgaagca gggatagttg ggcaactgtc tgcatcatcg
    22021 gagtagttgt cctctcaagt aatgcttgaa actgctgttt agctacttct tccttccctt
    22081 ccaaaatgga aacttcacct tgcataccta atacaccatc cgcaaaactc cctcgtgcat
    22141 cctcaggaac tgcttgaaca aagtctttta aatcatattc ttgaggagct agcaaggtct
    22201 gggcagaatg cctcaacatc aggtaggcgt atttggtatt ttcagggtgt tgtagtaatt
    22261 cccaaatttt tgctccatcg gtgatgtcga ctggcaaaat gttatttagg aagaaagata
    22321 aattaagaaa aatccaagtc cctgcaaaat accagcttct tgtcaaaaat ccaaacacta
    22381 tcgccaataa tatcaagccg agatgaacca tcaagcctcc tgaaagcatc aggatgattc
    22441 tttgatcgct ttcatcctct tttaaaccaa tgtattgagc accaacattt ttcagaatgg
    22501 ctgttctact aagatgaaac ctgcctgact ttttggtcaa aataaaatgt cctaatccaa
    22561 aagccaccag ccgatagcct gtcaagtagc cacaaaaagc atgacccagc tcatgaagaa
    22621 taaagattaa atacatgctt agaagagcga aggcataacc aaaagtaaag gctaaaactg
    22681 cggaataccc caactctgca aatgcgattg ttccacaagc aaaagctagc ataataaaga
    22741 caacagctag cacataaacc aaataagtcc caattttctt cataacacct ccaaccaact
    22801 cctagtatct tggataagga taaaattctc cctcttccaa gccaactttt ccttcttcaa
    22861 agacttcttg attccattcc ataacaaatt cctctgcttc tggatcttcc aaaaagtcca
    22921 tgagggtatc tagcccaacc tccgcagtat ctttgaggaa aagggcaaaa taagctaaaa
    22981 actcgcgaga aaatcctttt ttaggtagat aagggataac cgttaaatag tcttctgcat
    23041 tgacggttga cttggcagga ttgtagaaaa ggaccgcttc ctcaaaaaga atgtcatctg
    23101 atgaaacctc tccatcttca tccaccatct ccacacctgc agcattttgc gcttccaata
    23161 gaaaactcac ttctaccgcg tggttacgct tgtcccaact aatctcaaag tcaaagggaa
    23221 agttcttgtc caactcttcc tctaaaatat ctaaaaatcc gtatgttgcc attttgtcct
    23281 ctttctatgc gactctttaa tcgccccgat tgctcggaaa tatgctaaaa tagatactac
    23341 catcttacca caaaattatt ttatgtccta attataccat attacctcat ttaaaccctt
    23401 ggtatcagtg attttcttaa aagtctgatt tcttcatttc tcataaaaat caatataaaa
    23461 agccctcgaa agggctaata aatctataaa atcaataggc gagtaactag cacaagtgga
    23521 cgtgcttttt tattgactat taccacgata ccacgcttaa tcttaggctt gaactttctt
    23581 atctgcaata gcgtctgtca aagtctgaga aaagttaagt cccatttctc gtcccaactt
    23641 atctgcccat tttggtatgg tcaaagtctt tttaatgggt tcctgacttc ctaggtattc
    23701 tgatacatca acagatacca tagaaataaa agatttatca aggtcatagg ttgacacgaa
    23761 atcttcatca tctttaaaag gatcattatc aattaaagac aagctattga tatctgatgg
    23821 ctgaggtaac tctccatcac tctctatcaa atctgcaaca gttatcccta gccactccga
    23881 ccccatagcc aaagcctcag aaatcccctc tccttgtgta gctgagtatt caaaatctgg
    23941 gaaatggaca aaataagtcg cttctgttcc gtctgtgtcg tcataataaa ataaagctgg
    24001 atacgtaact aacatttcac tacctccata tcaaaaagca ggggctgaat tttacaaccc
    24061 agcttgcttt cttatccctc tttcagtgta cttattcagc tcaccatgaa ggattgtgat
    24121 aggtctttcc ccttgctttt ccattttaat atgggagcct ttaccgcctc tagtctttat
    24181 ccaaccatgg gccgtaagga gtttaaccat ctctttttgt gtcataggca tagcgctttt
    24241 acctcctgac aacaccatta taacacgtgt tacacgtatt gtaaaggagt gatacttatt
    24301 attctattat acataaaagc ccctagatgt ggttctaagg gaagccaatt tattcatacc
    24361 tatttttcta atgagtagta aaaactgctt ctttatcgag caattcatca tctgtatagt
    24421 caattgtaaa agtatctcga tctaagacag attgaggcgg agttgaatga atcataggaa
    24481 cactgcgtac tctatacttt ttatctccaa tttttacaaa ctgattaact ctcaagtctt
    24541 cgaaatcagg attttcaaca gttattacaa ggaggcgatt tactacttca aaaacatcaa
    24601 tta
//
LOCUS       DAYYQN010000031        23932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYYQN010000031 DAYYQN010000000
VERSION     DAYYQN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.031"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(14..3190)
                     /gene="carB"
                     /locus_tag="ACHBRQ_001179"
     CDS             complement(14..3190)
                     /gene="carB"
                     /locus_tag="ACHBRQ_001179"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9592858.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3502..4581)
                     /locus_tag="ACHBRQ_001180"
     CDS             complement(3502..4581)
                     /locus_tag="ACHBRQ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9592859.1"
                     /translation="MTKRILVLEDGTVFEGKAFGSDIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEIFKQSN"
     gene            complement(4631..5554)
                     /locus_tag="ACHBRQ_001181"
     CDS             complement(4631..5554)
                     /locus_tag="ACHBRQ_001181"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9592860.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5573..6094)
                     /gene="pyrR"
                     /locus_tag="ACHBRQ_001182"
     CDS             complement(5573..6094)
                     /gene="pyrR"
                     /locus_tag="ACHBRQ_001182"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9592861.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6305..6934)
                     /gene="nth"
                     /locus_tag="ACHBRQ_001183"
     CDS             complement(6305..6934)
                     /gene="nth"
                     /locus_tag="ACHBRQ_001183"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM9592862.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6934..7476)
                     /locus_tag="ACHBRQ_001184"
     CDS             complement(6934..7476)
                     /locus_tag="ACHBRQ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9592863.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7645..7961
                     /locus_tag="ACHBRQ_001185"
                     /pseudo
     CDS             7645..7961
                     /locus_tag="ACHBRQ_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
     gene            8200..9759
                     /locus_tag="ACHBRQ_001186"
     CDS             8200..9759
                     /locus_tag="ACHBRQ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9592864.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQSVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9908..10807)
                     /gene="htpX"
                     /locus_tag="ACHBRQ_001187"
     CDS             complement(9908..10807)
                     /gene="htpX"
                     /locus_tag="ACHBRQ_001187"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HFM9592865.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(10809..11369)
                     /locus_tag="ACHBRQ_001188"
     CDS             complement(10809..11369)
                     /locus_tag="ACHBRQ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFM9592866.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11463..12176
                     /gene="rsmG"
                     /locus_tag="ACHBRQ_001189"
     CDS             11463..12176
                     /gene="rsmG"
                     /locus_tag="ACHBRQ_001189"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9592867.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12430..13713
                     /locus_tag="ACHBRQ_001190"
     CDS             12430..13713
                     /locus_tag="ACHBRQ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9592868.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13908..15479)
                     /gene="ffh"
                     /locus_tag="ACHBRQ_001191"
     CDS             complement(13908..15479)
                     /gene="ffh"
                     /locus_tag="ACHBRQ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9592869.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15491..15823)
                     /locus_tag="ACHBRQ_001192"
     CDS             complement(15491..15823)
                     /locus_tag="ACHBRQ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9592870.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(15913..16290)
                     /locus_tag="ACHBRQ_001193"
     CDS             complement(15913..16290)
                     /locus_tag="ACHBRQ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9592871.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDRQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            16362..17666
                     /locus_tag="ACHBRQ_001194"
     CDS             16362..17666
                     /locus_tag="ACHBRQ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9592872.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            17679..18485
                     /gene="yidA"
                     /locus_tag="ACHBRQ_001195"
     CDS             17679..18485
                     /gene="yidA"
                     /locus_tag="ACHBRQ_001195"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9592873.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMVGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(18540..18611)
                     /locus_tag="ACHBRQ_001196"
     tRNA            complement(18540..18611)
                     /locus_tag="ACHBRQ_001196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18576..18578),aa:Arg,seq:cct)
     gene            complement(18653..19000)
                     /gene="rplS"
                     /locus_tag="ACHBRQ_001197"
     CDS             complement(18653..19000)
                     /gene="rplS"
                     /locus_tag="ACHBRQ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9592874.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19119..19448)
                     /gene="crcB"
                     /locus_tag="ACHBRQ_001198"
     CDS             complement(19119..19448)
                     /gene="crcB"
                     /locus_tag="ACHBRQ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFM9592875.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(19442..19816)
                     /gene="crcB"
                     /locus_tag="ACHBRQ_001199"
     CDS             complement(19442..19816)
                     /gene="crcB"
                     /locus_tag="ACHBRQ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFM9592876.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19813..20079)
                     /locus_tag="ACHBRQ_001200"
     CDS             complement(19813..20079)
                     /locus_tag="ACHBRQ_001200"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9592877.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20195..20638)
                     /locus_tag="ACHBRQ_001201"
     CDS             complement(20195..20638)
                     /locus_tag="ACHBRQ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9592878.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            20742..21677
                     /locus_tag="ACHBRQ_001202"
     CDS             20742..21677
                     /locus_tag="ACHBRQ_001202"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9592879.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21773..22015
                     /locus_tag="ACHBRQ_001203"
     CDS             21773..22015
                     /locus_tag="ACHBRQ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9592880.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(22072..22650)
                     /locus_tag="ACHBRQ_001204"
     CDS             complement(22072..22650)
                     /locus_tag="ACHBRQ_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HFM9592881.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKA
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESMV
                     RDNSTAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(22625..23556)
                     /locus_tag="ACHBRQ_001205"
                     /pseudo
     CDS             complement(22625..23556)
                     /locus_tag="ACHBRQ_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(23559..23932)
                     /locus_tag="ACHBRQ_001206"
                     /pseudo
     CDS             complement(23559..23932)
                     /locus_tag="ACHBRQ_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         7023 a         5391 c         4588 g         6930 t
ORIGIN      
        1 cttatttttc tatttaaatc gcttctgtaa caaagctacg actttcaagt accttaagca
       61 tggcattagc tgtatctagc gctgtgaaga ggggcacccc gtgttcaatg gctgaacgac
      121 gaatttgctc accatcttcg tcagcagttc gttttgttcc tactgtgtta atgatagctt
      181 gaattcttcc tttgcgtaca aaacttggga tatccttatc gtcatcacca atcttaccaa
      241 caggttgggc ttgcaagcca tgactagcaa agaaggctgc tgtcccttct gtcgcaagga
      301 ttccataacc aatgttttgg aaacgacgag ccaagttcaa ggcttcttct ttggcatcat
      361 cagcgatggt aaagacgaca ttaccaaaag ttggcaagtg tagataagaa gcttcaaagg
      421 ctttatagag agctttttcc aaagtagcat cagaacccat aacttcacct gttgacttca
      481 tttcaggacc gagcaagctg tctaccttag ctagtttggt aaaggagaag acaggtgcct
      541 tgatatgaac acgggtgctt tcagggtaaa gtccattttg gtagccaagt tctgataaac
      601 tttgaccaag aatgagcttg gtcgctactt gagccatagg aatattggtt accttagata
      661 ggaatggaac agtacggctg gcacgtggat tgacctcaat aacgtagact ttttcatcct
      721 tgataacaaa ctggatgttc atcattccaa ggcagtgaag accgattgct aagcgtttgg
      781 tgtagtctgc gatggtctcc tgaacctttt gcgacaaggt ttgtggtggg taaacagcca
      841 ttgagtcacc tgagtggaca ccagcacgtt cgatatgctc catgatacca ggaatgagca
      901 catctttacc atctgaaatg gcatcaactt cgcactcttg cccaacgata taagagtcga
      961 caagaactgg gtgatctgga ctagccttaa cagcagttcg catgtaagaa cgaaggtctt
     1021 cttcgttttc aacgatttcc atggcacgtc caccaagtac ataagatggg cggacaagaa
     1081 ctgggaagcc aatcttacga gctgcaagag ctgcttcttc ttcattggta gccgtttgtc
     1141 ctggtggctg tggaatatcc aattctttga gagcttgctc gaagaggtca cggtcttcgg
     1201 cacggtctag gtcagcaacc tgtgtaccaa ggatggtcac acctgctttt gccaatggct
     1261 ccgcaaggtt gatggctgtt tgaccaccaa actgaacgat aactcccttt ggttgttcca
     1321 agtcaatgac gttcataaca tcttcgaatg tcaatggctc aaagtaaagc ttatctgata
     1381 cagagaagtc tgttgaaacg gtctctgggt ttgagttcat gatgatagct tcataaccag
     1441 ctgcctggat agccttaaca gagtgaacgg ttgcgtagtc aaactcaacc ccttgaccga
     1501 tacggattgg acctgaacct aggacaagta cagattcttt atcagatctg atagattcat
     1561 tttcccaacc ataggttgaa tagaaatatg gcgtttcaga gtcgaactct gccgcacaag
     1621 tatctaccat cttataaact ggaacaatct tgttttccaa gcgaagttgg cgaacttgat
     1681 catcagtcgt tccccagagt tcagcaatct tacggtctga gaaaccatta agtttggctg
     1741 ttttcaaaac ttctagatct tgtggatgag cacccaattc ttgctcaatt tcaaagatat
     1801 gcaagagttt atcaagatag aagatatcaa tttttgtaag ctctgcaatt tcttcaggtg
     1861 tgtagccacg acgaatggct tctgatacgt agaagagacg gtcatcttgg gctttgacaa
     1921 ccttttcaat caaggcatca tcagaaactg ctgcaagttc aggtatttca ttgtggtgca
     1981 ccccaatttc aagggagcgg caggccttga gaagagattc ctcgatgtta cgaccgattg
     2041 ccatgacttc tccagtcgcc ttcatttgtg taccgagacg gcgttcaccc ttttcaaact
     2101 tgtcaaatgg gaaacgtgga atcttagcaa ctacgtagtc aagggctggt tcaaacatgg
     2161 cataggttga acctgtaact gggtttataa cctcatccaa ggtcaaacct actgcaatct
     2221 tggcagccaa cttagcaatc ggatatcctg tcgctttaga agcaagggct gacgaacgtg
     2281 atacacgagg gtttacttcg ataacataat acttgaagct gttaggatca agagctagct
     2341 gaacattaca tccaccttca atcttgaggg cgcgaataat gctcaagctc gcatcacgaa
     2401 gcatttggtt ttcatagtct gacatggttt gcgcaggggc aaatacaatg gaatcccctg
     2461 tgtgaatccc aactgggtca aagttttcca tgttacaaac aaccaaggca ttgtcagctg
     2521 agtcacgcat cacttcgtat tcaatttcct taaaaccggc aatcgaacgc tcaatcaaac
     2581 attgggtaac aggtgacaat ttcaaaccat tttcagtgat ttcacgcaat tctttttcgt
     2641 tggcacacat accaccacca gtaccaccaa gggtaaaggc tggacgaacg atgactgggt
     2701 agccaattgt cgctgcaaag gcaactgctt cttctactgt gttaacaatt tcagattctg
     2761 gaatgggttg ctcaagctct tccatcaatt gtttaaagag gtcacggtcc tccgcttggt
     2821 caatggcaga taatttggta cccagaagtt caacgccaag ctcgtctagg ataccatttt
     2881 tagataattc catggccatg ttgagacctg tctgaccacc gagtgttggt agcaaggcat
     2941 ctggacgttc cttacgaaga atacgtgtca caaactcaag tgtaatcggt tcaatgtaaa
     3001 ccttgtcagc aatttccttg tccgtcatga tggttgcagg atttgagtta accaaaacaa
     3061 cctcataacc ttcctctttc aacgacaagc aagcctgggt cccagcgtaa tcaaactcag
     3121 cagcctgacc aataataatc ggaccagaac caatcaccat aattttttga atatcagtac
     3181 gtttaggcat agtttatgat acaaagcccg taaagaacac agtgaaaata ggaaactcgg
     3241 cgcagacgcc ttcagcgtca agacgatgtt tatctttttc acacagttct tagggcgtgt
     3301 tcacttccgc gaacaatgta tcttctcctt tctattcgtc gcctctcagg gcgacattaa
     3361 ataagataca aaggacgaat agaaagcgat tgaattttag gaaatcaagg aaggattgac
     3421 aatccaagtt ggtttctcta aattctgagc tttccgtccg tgttcagtta cataaattct
     3481 ccgacgagct tttactcgtt cttagtttga ttgtttaaaa atttccatca tctcgataaa
     3541 ctcgtcaaat aggtagctag cgtcgtgtgg tccaggagct gcatctgggt ggtattgaac
     3601 agagaaagca ggttggtatc tgtggcgcac accttccact gacttgtcat tgatttcttc
     3661 gtgggtaata atcaagtgct ctggcaaatc ctcgcggctg actgcataac catggttctg
     3721 gctggtgaaa tctactcgtc ctgttgcgat ttcacgtacc gcatggttga atccacggtg
     3781 gccaaacttc atcttatagg tcttagcccc gtttgccatt gcaaagagtt ggtgtcccat
     3841 acaaatacca aagattggaa tttttccttg tacaccgcga atcatgtcga gtgcttgtgg
     3901 aacgtcttct gggttacctg gaccatttga caacataact ccgtcaggat tgagatggag
     3961 aatttcttca gccgttgtcg aataaggaac aactgtcacg ttacagttgc gtttagaaag
     4021 ttcacgtagg attgagtgct tgagaccaaa gtccactagc accacgctca aaccaactcc
     4081 tggagctgga taagacgttt tagtagaaac ctgtttgata ttgtctgtcg gtaaaactgt
     4141 tgcttggagc tggtccgtca catggtccat actgtcccca acatgggtca aggttgcacg
     4201 catagtacca tgcttacgga taatcttggt aagagcacgc gtatcaattc ctgaaatccc
     4261 tggaattttc ttggctttca aaaattcatc caaggtcatt tggttgcgcc agttgctagc
     4321 tctacgcgct tcttcaaaaa caacgactcc cttacaagtt ggaataatgg attcataatc
     4381 atcacgatta ataccataat ttcctaccaa aggataagta aaggtcaaga tttgtccatt
     4441 ataagactgg tctgtaatgg attcttggta gccggtcatc cctgtattaa agacgatttc
     4501 gcctgttaca tcaatatctg atccgaaggc cttgccttca aaaactgtgc catcttctaa
     4561 tactagaatt ctttttgtca tattttcacc tctcgtggac gctcactggc gtcttttaac
     4621 gtcttgtgtt ttagttggcg tttctactcg ctagtacgga ttctaagatt gccattcgaa
     4681 caaagacacc attggtcatt tgttggacaa tccgtgattt tggtgcttca accaagtggt
     4741 ctgctatttc tacatcacga ttgattggag ctgggtgcat gaggattgct gtttctttca
     4801 aacgatcgta acgttcttga gtcaagccat gttgggcatg gtagtcttct tttgaaaata
     4861 cagctccact atcatggcgt tcgtgttgca cacggagaaa catcatgaca tcaacctgat
     4921 caatgatttc atcaatggtt acaaactgtc catagtctgc aaactcttga cttctccatt
     4981 cctcaggtcc agcgaaaaag agttcagatc ccaagcgttt caaaatctgc atattggatt
     5041 tggcaacgcg tgagtggtcc aagtcacctg caatagcaac tttaagaccc tcaaagtggc
     5101 caaattcctc ataaatggtc atcaaatcaa gcaagctctg gctagggtgt tggcccgaac
     5161 catctccacc attgatgatg gaagtcgtaa tcgttggact agcaatcaat tctctatagt
     5221 agtcgacctc tggatggcga atcacacaga catccactcc taaagcagac agagtcaaaa
     5281 tggtgtcata aagtgtctca cccttattaa ccgaactagt cttcacatca aagtcaagtc
     5341 gttccaatcc aagtttaatc tctgcgactt caaaggactt atgtgtccgt gtagaatcct
     5401 caaagaagag attggaaaca atcggatggt cttcataggg aagctgggct ccatttttaa
     5461 actcaattcc tcgcttgatc aatttcatta cttgatcgac agtgaggtct tccatggaca
     5521 ccacatggtt caatgcttgt tgattttctg acatggctac tcctttaact ttctaagctt
     5581 cttcagtaat cagaactctg tcttggtcat caagttctgt catctctacg atgatttctt
     5641 cagaacgact ggttgggata ttttttccaa cgtaatctgg acggattggc aattctctat
     5701 gtccacgatc gactagaact gctaaactca cacgcgcagg acgaccatga ccgacaatat
     5761 tatcaatagc agcacggatg gtacgacctg tatagagcac atcatccacc aagataactt
     5821 cgcggtctgt cacatcgaca gaaaccaaag aagtatcttc tccactttta acatcatcac
     5881 ggaaaggttt agtatccaat tccacaacag gaactgaaag attttctaac tgcttcaaac
     5941 gttcttggat tcggtgggca ataaagacac cacgagtttt aataccagcc aagacgatct
     6001 tattcaaatc tttgttgcgt tcgataatct cataagtaat acgcgtaatc gctcgtttga
     6061 cggtcaattc gtctacaact tcttttgttt tcatgacaaa cctccaaaaa gaaaagtctc
     6121 cttaaacaag gagacttgaa atttatagcc aagcgagccc tactgcacac agtatagact
     6181 tcacccttct actttatcgc gctccttgcc tgcctcacgg gacaggttta aaggaatatt
     6241 tagttatcat ttactatagc acaaagcatg cttaaaatca agcaaaaagt ttcaatgtag
     6301 catcttacaa attgctaaaa tcatataatt gtgggtactg gtcacactct ggattttttg
     6361 gatggcaaat ggctcttcca aaataaatca tggcctgatg ggcagctaac cactgctcag
     6421 gcggcaagat atccatgacc cgcttttcca cctcaagtgg cgtcgctgat tttttgacaa
     6481 tatcgtggtg tttgcaaata cgctccacat gagtatccac tgcaaaggct ggaatcccaa
     6541 atcctacact catgacaaca ttggctgtct tgcgaccaac acctgccaaa ctctccaatt
     6601 cttcacgtgt ctgagggact tgaccatcaa aatcgtctag taactgttgg gcacattttt
     6661 taaggaattt agctttattc cgatacaatc ccaagcgaga aatatgtgaa gcaatctcac
     6721 tctctgtcgc tacagacata gcttggggtg ttggaaaggc aacaaagaga cctggtgtgg
     6781 ccttatttac cgctgcatct gtcgtctggg ctgataacat gaccgcaacc aggagttcaa
     6841 aatgattggt aaaatcaaga ctaggcttgg catctgggaa gagggcaatg atttcttcta
     6901 gcacctttcg tgctcgtttt tttgacaaga ccattattca tctccgtcaa atagtccttg
     6961 taagccagca aaaggactgt tttcttcttt ctttactgct tgttgagctt ggtattcttc
     7021 ctctgtcatg atttgccagt catttcctga gacaaatcct tgaccagctt cttcttcagc
     7081 cgtcaagacc ttgataggaa tgtttagcag gatattgtct gatacactct cagcaaggtc
     7141 aagcgcccca ttttcgatgg gcaagaccaa gtcatcatct aaaacttctt gatctagctg
     7201 gttagttgcg ccttccatga aaacttccgt gactggataa gattcaacta actcaactgg
     7261 ctccatactg cgactcgaag caagaacaat ggtataagat agttgataat ctaagaaata
     7321 catacggtct tcatattgta ctttcccaac tgcaaggata tcttttacat ctaaaatttc
     7381 ttgattacgt gcacgcaggt catcaactaa atctaacgtt tgttcaaagt tcaaaccttc
     7441 agactgctta cgaatttctt gaatatttaa tttcatactt cctccataaa gatttactct
     7501 cttgattata ccatgaaaag gctacaaatc agcacaccaa actttgtaat taaaattcaa
     7561 attttaacat atttactatg atagttttat tttttagtgc tatactatag ggaaagagta
     7621 catcagatca aggaggatgc tcacatggaa gacaagaaac tcattcaact cctatccaag
     7681 ttaaataaaa gctaccaaaa ctgtaaacag ggtacggcag atgatattcg actacaagag
     7741 ctgctaaaca ctactatgca agagctcaaa aaacggaaaa gttgaacaac agtatcttaa
     7801 ttgatcttga gaaattttac caacccacca gtcttctgat tggactgggt agcctaaaac
     7861 taaacgatca agcacgcact gcttggcgaa actatgataa attccattac gagcatgtca
     7921 aacacgtact aagtctctat ggacctgttt ttggatttta gagcatagaa tttccagttt
     7981 tctgttgaca aaatttcctt aaaggtataa tataaagata ctaatactcg gaggtaaggg
     8041 agacatgaac aactaagtct atcaaataaa gaacctttat ttagtagatc ttgtttttgt
     8101 ctctttttgt gtgctctttt atgctctttt tctggcatgt taatagagtt tttttgacat
     8161 agactttggg ctctactagg taaagtagag ctttttgtta tgcactatga acattctaga
     8221 aagggaaatc atatgataaa aatcaatcat ctaaccatca cacaaaacaa agatttacga
     8281 gatcttgtat ctgacctaag catgaccatc caagacgggg aaaaggttgc tattattggt
     8341 gaagaaggaa atggcaaatc aaccttactt aaaattttaa tgggggaagc tttgtctgat
     8401 ttcactatca agggaaacat ccaatctgac tatcagtcac tggcctacat tcctcaaaaa
     8461 cttcccgagg agctgaaaaa gaaaacttta cacgactact tctttttaga ttctattgat
     8521 ttagactaca gtatcctcta tcgtttggcg gaggaattgc attttgatag caatcgtttc
     8581 gcaagtgacc aaaagattgg caatctatca gggggcgaag ctttgaaaat tcagcttatc
     8641 catgagttag ccaaaccctt tgagattcta tttttagatg aaccttcaaa tgacctagac
     8701 cttgagacag ttgattggct aaaaggccag attcaaaaga ccaggcaaac cgttattttc
     8761 atttcccatg atgaagactt tctttctgaa acggcagaca ctattgttca cttgcgactg
     8821 gtcaaacacc gtaaagaagc ggaaacgcta gtagagcatt tagactatga tagctatagt
     8881 gagcagagaa aggctaattt tgccaaacaa agtcagcaag ctgctaacaa ccaaagagcc
     8941 tacgataaaa ccatggaaaa acatcggaga gtcaagcaaa gtgtagaaac tgcgcttcga
     9001 gctaccaaag atagtactgc cggtcgccta ttggctaaaa agatgaaaac tgtcctctca
     9061 caagaaaaac gctacgaaaa ggcagctcag tccatgactc aaaagccact tgaagaggaa
     9121 caaatccaac ttttcttttc agacatccaa ccattaccag cttctaaagt cttagtccaa
     9181 ctggaaaaag aaaatttgtc cattgacgac cgagttttgg ttcaaaaact acaactaact
     9241 gtccgtggcc aagaaaaaat cggtattatc gggccaaatg gtgttgggaa atcaactctg
     9301 ttagccaagt tacagagact tctgaatgat aaaagagaga tttcacttgg ttttatgcca
     9361 caagattacc acaaaaaact gcaattggat ttatccccaa tagcctatct cagtaaaact
     9421 ggggaaaaag aggaactaca gaaaatccaa tctcacctag ctagtctcaa tttcagttat
     9481 ccagaaatgc agcatcaaat tcgctcctta tctggcggac aacagggaaa actcctgctt
     9541 ttggatttag tcctgcgcaa accaaacttt ctcctgctgg atgaacccac acgaaacttt
     9601 tctcccactt ctcaacccca aatcagaaaa ctctttgcca cttatcctgg cggtctcatc
     9661 actgtttcgc atgaccgtcg tttcttaaaa gaagtctgct cgatcatcta tcgcatgaca
     9721 gaacacggtt tgaagctagt taatttagaa gatttataaa tttgcaacat agcagatagg
     9781 tggggcaaaa acacaatttt aggagaaaaa gaagtaaatc ttcccatagt aaaacgcata
     9841 atatcaagtt ttcacacctg atattatgcg tttttcagat tttaaagact tttttccagc
     9901 cttcatttta catatgtttt aaacgttcaa tccgttctga gataggtggg tgggtataaa
     9961 agagtttttg gaacccccca cctttcttgg gatcattgat ataaagggca ctgctagcat
    10021 catcgacgtg gcgactcata ggtttgctat tgtccaactt atctagggca ttaatcattc
    10081 cctggggatt gcgagtcagc tcgacactag atgcatctgc cagaaattcc ctctgacgag
    10141 aaatagcgag ctgaaccaag gttgcagcga gaggtgccag tacaatagct agtagggaaa
    10201 ccactagcat aatgatttca agaccatttc catctcggtc atcatcactt cgtctgcgac
    10261 ctgctccacc ccaccacatc atacgacctg ccatactaga aagcatggtg atagcactag
    10321 caagggcaac tgcaatagtc gaaatacgga tatcataatt acgaatatga ctgacttcat
    10381 gtcccataac agcttctagt tcttcacgat tcatgatagc tagtagacct gaagtcgcag
    10441 caacagccgc attttgagga ttagaacctg tcgcaaaggc atttaaggct ggatcatcaa
    10501 tgatgaaaac acggggcata ggaatctgag cgaccagagc catatcttcc actacatggt
    10561 agaggtctgg tgccgtttgc tcatccacct cacgcgctcc attcatggac atgacaatct
    10621 ctgtcgattg aaaaatcata gacaaagcgt agataaagcc gataatcagt gcgataacca
    10681 aaccaccaag tccagatctt ataaagagat aaccaaccgc ataaccaaca agagctaaga
    10741 gtaggaaaaa taccagcaac aaaatccagg tttttcgttt attgcttgca atttgatcaa
    10801 acaacatctt agtcacctaa accgctaaaa tcaactttag gaaccgactt ttcctcttca
    10861 ggtgtttgaa ggaaatctgc cgctttaaat ccaaacattc cagcgataat attgctcggg
    10921 aaagtttcta attttacatt gtagttgctg acaacactgt tatagagttg acgagagtaa
    10981 gaaattttat tttctgtgtt tgtcaactcc tcttgcaatt taacaaagtt agcactagct
    11041 ttcaaatctg gatagctttc tgcaactgca aaaatacctg aaacctgacg agtgagggca
    11101 tcactggctt tcatagcttc tgctggtgaa gtcgctgccg ccacttggtt acgtagttct
    11161 gccacctttt caagggtaga accttcatat ttggcataac cttttacagt ctcaatcaag
    11221 tttggcaaga ggtcatttcg acgtttcaac tgaacatcaa tctgactcca agcctccttg
    11281 gtttgcatac gatttttaac caaaccgtta tagctaacaa tcacaaaaat aacaacaaga
    11341 gctaaaactc caagaataat ccaagtcata atataagtcc tttctgcttt tagattagta
    11401 ccagtatatc aaattttcta tgattgtggt aaaataagat gatactaaag aaggaaataa
    11461 ctatgaaacc aaaaacattt tacaacttgc ttgccgagca gaatcttcca ctttcggacc
    11521 agcaaaaaga acaatttgaa cgttattttg agctcttggt cgagtggaat gagaagatta
    11581 atttgacggc gattacggac aaggaagaag tttatctcaa acatttttac gattcgattg
    11641 cacccattct tcaaggtttg attcccaatg aaactatcaa acttcttgat atcggggctg
    11701 gggcaggatt tcctagtcta ccaatgaaaa ttctctatcc ggagttagat gtgaccatta
    11761 ttgattcact caataagcgc atcaacttcc tacaactctt ggctcaagaa ctggatttga
    11821 acggagttca tttctaccac ggacgtgccg aagattttgc ccaagacaag aacttccgtg
    11881 ctcaatatga ttttgtaaca gctcgtgcgg ttgcccgtat gcaggtccta tctgaattga
    11941 ctattcccta ccttaaggtt ggtggcaaac tattggcact caaggccagc aatgcgcctg
    12001 aggaattatt agaagctaag aatgccctca atctcctttt tagtaaggtc gaagacaatc
    12061 tcagctacgc cctaccgaat agagatccgc gctatatcac agtggtagaa aagaaaaaag
    12121 aaacaccaaa taaatatcca cgtaaggctg gtatgccaaa taaacgccca ctttaaattt
    12181 tttagtaaac aaatgtttac aaaatcagcc tcgctctttt atttctaggc tcgggaaaaa
    12241 atgatttaca aaatcatttt tttctgctat actatcctaa gcaaaggttt ttaatgtcat
    12301 cccgtgaggt gacgaagacg caaaaatatt tgaaactctt taaagtctag attttagaga
    12361 agtcttactc tgagggccta ttgctgtaaa ataatgggct cttttctgat gcccaaaagt
    12421 gaggtttata tgaaacaaga atcaactgtt gatttgttac tagacgttga ccaacgtcct
    12481 tcggatggaa aaggaattct ccttagtttc caacacgttt tcgccatgtt tggtgcgacc
    12541 atcttggtac cattgatttt gggaatgcct gtatctgttg ccctttttgc ttcaggtgtt
    12601 ggaacactca tctacatgat tgctactggt tttaaagttc cagtttatct aggttcttca
    12661 tttgccttta tcacagctat gtcactggct atgaaagaaa tgggggggga tgtatctgct
    12721 gcccaaacag gggttatctt gactggtttg gtctatgtcc ttgttgctac cagcatccga
    12781 tttgtaggaa caaaatggat tgataaactc ttgccaccaa tcattatcgg tcctatgatc
    12841 atcgttatcg gtcttggact tgcaggttca gctgttacca atgcaggtct tgtagcagac
    12901 ggaaattgga aaaatgctct ggtagccgtt gttactttcc taattgctgc ctttatcaat
    12961 acaaaaggaa aaggcttcct acgaatcatt ccattcctct ttgccattat cggtggttac
    13021 cttttcgcac taactcttgg cttggttgac tttacaccag ttcttaaagc caactggttc
    13081 gaaattcctg gtttctactt gccatttagc acaggtggtg cctttaaaga gtacaatctt
    13141 tactttggtc cagaagccat cgctatcttg ccaatcgcta tcgtaacaat ttctgaacat
    13201 atcggagacc atactgtttt gggtcaaatc tgtggtcgtc aattcttaaa agaaccaggt
    13261 cttcaccgta ctcttcttgg tgacggtatc gcaacttctg tttctgcctt ccttggtgga
    13321 ccagccaata caacttacgg agaaaataca ggggttatcg gtatgactcg tatcgcttct
    13381 gtctcagtta tccgtaacgc tgccttcatc gcgattgccc tcagcttcct tggtaaattc
    13441 actgccttga tttcaactat tccaaacgct gtacttggtg gtatgtcaat ccttctctat
    13501 ggggttatcg ccagcaatgg tttgaaagtc ttgattaaag aacgtgttga tttcgctcaa
    13561 atgcgaaacc tcatcatcgc aagtgctatg ttggttcttg gacttggagg agctatcctt
    13621 aaacttggtc cagttacact ttcaggtact gccctttcag ccatgacagg aatcatcttg
    13681 aacttgatct tgccatacga aaataaagac taagagtcta aatacaccta atccactcag
    13741 acagctgagt ggatttttcg cataccataa taaaagtgtc ttaacaaaat tattaaaatc
    13801 aaaaaacgta taatatcaga tattctaaaa ccttgatact gtacgtttta tcatagaaat
    13861 ttttacttta ttttctcatc aaatgagatt tgcatcaatc tcttgtctta cttgcgtttc
    13921 ttcttcgctt tcttcatttt gttagccata cgtttcatgg actgtttcat ggcaaattca
    13981 ccaattttac ctttcaaacc gccaccaaac atctggctca tatctggcat tcctgctcct
    14041 ccgagagctg ataagtcagg cataccgcct tgtcccatca ttccttcaag ggcagacata
    14101 tccattcctc ccatatttgg catattttta ggaaggttat ttggattaat ccccatttgc
    14161 ttcatcattt tattcatatc cccagacata acaccctgca tgagctgttt agcctggtta
    14221 aagtccttga tgaatttatt gacttcgacg aatgtatttc cagaaccagc agcaatacga
    14281 cggcgacggc ttggatttaa caaatctggg ttttcacgct cttcaggtgt catcgaagac
    14341 acaatggcac gtttacgagc aatctggcgt tcatccacct tcatgttttg aagggctgga
    14401 ttgttggcca tacctggaat catcttgagc aagtcttcca tcggccccat attttgcacc
    14461 tgatctaatt gatcgatgaa atcattaaaa tcaaaggtgt tttcgcgcat cttctcagcc
    14521 atttcaaggg ctttttgttc atcgtattcc tgagaagctt tctcaatcaa agtgagcata
    14581 tcccccatac caaggatacg gctagacatg cggtctgggt ggaaggtttc aatgtccgta
    14641 atcttttcac ctgtaccagt gaacttgatt ggttttccag taatgtgacg aacagacaga
    14701 gcagcaccac cacgagtatc gccatcaatc ttggtaagga tgaccccagt cacttccaac
    14761 tgagcattaa actcacgcgc aacattggct gcttcctgac caatcatagc atcaacgaca
    14821 agcaagattt catttggttg agccaatgct ttcacatcac gaagctcatt catgaggagc
    14881 tcatcaatct gcaaacgacc cgcagtatca atcaagacat agtcgttatg attagtttgg
    14941 gcttgctcca aaccttgacg tacaatctca acagctggta cttctgttcc aagtgcaaag
    15001 acaggcacat caatctgttg tcccaaggtc ttaagctggt caatggcagc tggacgataa
    15061 atatccgccg caatcatcaa aggacgagca ttttcttctt tcttgagttt gttggccaat
    15121 ttaccagcaa aggttgtttt accagcccct tgtaaaccaa ccatcatgat gatggttgga
    15181 atcttaggtg acttgataat ttctgccgta tcagaaccta aaacggctgt cagttcctca
    15241 tcaacgattt taataatctg ttgcgcagga ttaagtgtat caatgacctc atgcccgact
    15301 gcacgctcac gaactttctt gataaagtcc tttacaacag gcaaggcaac gtcggcctcg
    15361 agcaaggcca agcgaatttc tttggttgcc tcttggacat cagattcaga gatttttcct
    15421 tttttacgta gatttttaaa gacgttctgc aaacgttctg ttaaactttc aaatgccatt
    15481 tttcttcctc ttattctcta ttatcaatgc ttgttaaaat ttctatctgc tcctgcagaa
    15541 agttatcctt gggatagcgc tccaaaatct gatcaaaaat ctgactgcgg acaatatagt
    15601 ccgagtacat gtgcaatttc atctcataat cttccagaat cttttctgtt cgcttgatat
    15661 tgtcatagac agcctgacga ctgacaccga actcctcggc aatttcagca aggctgtaat
    15721 catcagcgta gtagagctcg atataattca tttgcttatc tgtcaaaagc gccgcataaa
    15781 attcaaagag cgcattcata cgattggttt tttcgatttc cataactttt attataccaa
    15841 aaaatagcct aatctaccac actagggagc caatccttga agatagaaag tagatttgag
    15901 aaaaacgaga tcctagcccc aagtaatttc caattgatag ctggcaaagg gatgcccctc
    15961 ttgattttgt agttgataag ctagctcaat cttttgccta tcaacttgat aacggctcgt
    16021 ttgaatgata aattcctgca tgcccatagg ggtaggaata taggccaaac tatcactatc
    16081 ctttaaaaag cgcataatgg tcttgggatt agaaaatcgg ctcatcacca gttcttgacc
    16141 atgaaattta ataactactt tttccttttc ctcattataa aagagtaaat agctataatc
    16201 tcccttttca tgcacttcca catcataaag ctggtcaatc acttccaact gctcatcaaa
    16261 ctgaatcgta tttcgcatcc gaatcttcac atcaggtcct ctttcttgtc tcttgtccta
    16321 ctattttacc aaaaagagca ggattttgct ataatggtca tatgaacgaa aaagtattcc
    16381 gtgaccctgt tcacaactac atccatgtca ataatcaaat catctatgac ttgattaata
    16441 caaaagaatt tcaacgtttg cgccggatca agcaactagg gacttccagt tataccttcc
    16501 acggtggaga gcatagtcgc ttctctcact gtctgggagt ctatgagatt gcacgacgta
    16561 tcacagaaat ttttgaagaa aaatatcctg aggaatggaa tcctgccgag tctctcttga
    16621 ccatgaccgc tgctctccta catgaccttg ggcatggcgc ctactcccat acttttgaac
    16681 atctctttga tacagaccat gaagccatta ctcaggagat tattcaaaat cctgagacag
    16741 agattcacca agtcttgcta caagtagcgc ccgatttccc agaaaaggtg gccagtgtca
    16801 ttgaccatac ctatcctaat aagcaggtcg tgcagctcat ttctagtcag attgacgcag
    16861 atcgcatgga ctatctcttg cgcgactcct attttacagg agcatcctat ggggaatttg
    16921 acctgactcg catcctccga gtcattcgtc ctatcgaaaa tggtatcgcc tttcagcgca
    16981 atggcatgca cgccatcgaa gactacgtcc tcagtcgcta ccagatgtac atgcaggttt
    17041 atttccaccc cgcaacacgc gctatggaag ttctcctaca gaatcttctc aagcgcgcta
    17101 aggaactcta ccctaaggac aaagacttct ttgcacgaac ttccccacac ctcctgcctt
    17161 tctttgaaaa aaatgtgacc ttgtctgact atctggctct agatgatggt gtgatgaata
    17221 cctacttcca gctctggatg accagtcctg acaaaattct cgcagattta tcgcaacgct
    17281 ttgtcaaccg caaggtcttt aaatccatta ccttttcaca agaagatcaa gaccaactcg
    17341 ctagcatgag aaaattggtt gaggacattg gctttgatcc cgactactat actgccattc
    17401 ataagaactt tgacctccct tatgatatct atcgtcccga atctgaaaat ccacggacac
    17461 agattgagat tttacaaaag aatggagaac tggccgaact ctctagcctg tctcctatcg
    17521 tccaatccct tgctggtagt cgtcacggag ataatcgttt ctattttccg aaagaaatgt
    17581 tagatcaaaa cagtatcttt gctagcatta cccagcaatt tttatacttg attgagaacg
    17641 atcattttac cccaaataaa aactagaaga ggaaatttat gagtattaaa ctaattgccg
    17701 ttgatatcga cggaaccctt gtcaacagcc aaaaggaaat cactcctgaa gtcttttctg
    17761 ccatccaaga tgccaaagaa gctggtgtca aagtcgtgat tgcaactggc cgccccatcg
    17821 caggtgttgc caaacttctg gacgacttgc agttgagaga ccaaggtgac tatgtggtga
    17881 ccttcaatgg tgcccttgtc caagaaactg ctactggcca tgagattatc agcgaatcct
    17941 tgacctatga ggattatctg gatatggaat tcctcagtcg caagctcggt gtccacatgc
    18001 atgccattac caaggacggt atctatactg caaatcgcaa tatcggaaaa tacactgtac
    18061 acgaatcaac cctcgtcagc atgcctatct tctaccgtac ccctgaagaa atggttggca
    18121 aagaaattgt taaatgtatg tttatcgatg aaccagaaat tctcgatgct gcgattgaaa
    18181 aaattccagc agaattttac gagcgctact ccatcaacaa atctgctcct ttctacctcg
    18241 aactccttaa aaagaatgta gacaagggtt cagccattac tcacttggct gaaaaactcg
    18301 gattgaccaa agatgaaacc atggcaatcg gtgatgaaga aaatgaccgt gccatgctgg
    18361 aagtcgttgg aaaccccgtt gtcatggaaa atggaaatcc agaaatcaaa aaaatcgcca
    18421 aatacatcac caaaacaaat gacgaatccg gcgttgccca tgccatccga acatgggtac
    18481 tgtaaaagga aagaatagac taaaaccgct cagctgagcg gtttttgatg tctaattact
    18541 gccccctgca ggaatcgaac ctgcaactac tccttaggag ggagttgtta tatccattga
    18601 actaagggag ctagataaaa actctgctga aaagcagagt tttttagtcg aattaacgac
    18661 ggatttcttt gatacgagct gctttacctt gaagagcacg caagtagtac aatttcgcac
    18721 gacgtacttt accgtaacga acaacttcga ttttttcaac acgtggagtg tggattggga
    18781 agatacgctc aacacctaca ccgttagaga ttttacgaac tgtgtagttt tctgagattc
    18841 cagcaccttt acgtgcgata acaacacctt caaaaatctg gatacgttca cggttacctt
    18901 cgacaacttt cgcgtgtaca cgaacagtgt caccaggacg gaatgatggg atatctgtac
    18961 gaagttgacc ttcagtcaag ctttggatta atggattcat tttattctcc tatctttgtc
    19021 aatcttgagg aaacttcctc agcggataaa ctgtattttt gtgcgtccat tacacacaag
    19081 atacagttta ccaaatttct aaaaaaaagt aaagaaattt atagcaaaat tcctaaaaaa
    19141 atcgcaacca aaccacctat gtaagtcaaa gtcaggtaag aataaaatac cttcttatcg
    19201 cttagcagtc tttgaagttc gtcattcaag gtcgaaaaag ttgttaaacc gccacaaaat
    19261 cctgttgcca gaatagcata tacttcctta gactctacat gattgtagaa tactccaatt
    19321 aaaaaacaac ctaaaagatt agctatgagc gttccgagtg gcaatttaga agcttgatta
    19381 tagcgagaaa agaaataccg tacgagtgct cccaagccac aggcgattgc aagatagacg
    19441 attaccattt cttcctccct agaaaataag ctaaaagcag gcctccaccg atactcaaaa
    19501 gcaaatacag tatcaaacta agataacgcc ctgtatcaag cagcttcaca gtatcaagca
    19561 ttaggctgga aaaagttgtt aaacctccgc aaaaacccgt ccccagtgct aaaatcaagc
    19621 ccttactagt ccccttatag accagatagc ccttgacaag ataaatcaag cagaaaattc
    19681 ccagatagtt gacaaagaga gtgccccaag gaaagtctgg actggctggt aaccaggtgg
    19741 aaactagata tcggacaagt ccgcccaaca tagcagctag aaaaattcct agcggataaa
    19801 attgttcttt tttcatttga tattttgatc ctgataatca cgcgaacgtt tgagtatgtc
    19861 cgaaaaagtc gctacaatag tctcctgata gcgcttgtcc tctacacgac tcctaacctt
    19921 ttcaaaaata acttcttctc tcttagtatc taaaatcggt ttacctgaag ctttcttata
    19981 agcaaccacc ccctcaacta aatgcattcg ttcttctaac agtttgacaa tttggtcgtc
    20041 gatttgatcg atttcttgac gaataatatc taaatccata cagtctcctc ctttatttga
    20101 attattgtat caaaaagcca tcaattaggc tagtaaaatc ccaactctcg ataagaaaaa
    20161 tgcactgatt gattacatca gcgcactttt atgcttatcc tactttagca accaattctt
    20221 ctgcgaattg ttccaagcgt tcaatatctt cttcctcggc agaaagatca actttgacac
    20281 actcagaacc tttttctgct cctgttgaaa caaagacacg gtcaaagtca tcaacagcct
    20341 tacagaattc atcgtagaag gtatctcctg agccgaccac tccgtagatt ttgccattca
    20401 agttgagatc tgctagatct tcgtagaagt ccatcatctc atctggcaat tcaccatcac
    20461 cataagtata ggtcgcaacg atagcgatgt ctgcttccaa gaagtctgaa gcgtcaacag
    20521 ttgtacattc atcaacatca acatccaagc ccaagtcacg taatttgtct gctacaatat
    20581 ctgcaatttc ttcggtatta ccggtcatac tggcaaatac aatttttgct aatgccatat
    20641 cgtcctcctc aatttatctt tctccattat atcacatctt tttcatttta aaaataaaaa
    20701 ttcttgtgct acaatgaaat gagaaagaat tgaggttatt tatggagatt tgccaacaaa
    20761 ttttagaaaa aattaaagaa tacgacacca ttatcattca tcgtcatatg aaaccagacc
    20821 ctgatgcctt gggaagtcag gtgggattga aagccttgct ggaacatcat ttcccagaaa
    20881 aaaccatcaa agccgtcggt tttgatgaac caactcttac ttggatggct gagatggatc
    20941 ttgttgaaga tagagcctac caaggcgcac ttgtcatcgt ctgtgataca gctaatactg
    21001 ctcgtatcga tgataagcgc tatagtcaag gtgattttct cattaagatt gaccaccatc
    21061 caaatgatga tgtatacggt gacctgtctt gggtcgatac tagttcaagt agcgctagcg
    21121 agatgattac cctatttgcc caaacaaccc aactagcctt ggcagatcgc gatgctgagt
    21181 tgctctttgc aggaattgtc ggtgatacag gtcgcttcct ctacccttct accactgcac
    21241 ggactcttcg cctggctgct tatttgagag aacataactt tgactttgcg gctctcactc
    21301 gcaaaatgga cactatgagc tacaaaattg ctaaactgca aggctacatc tacgaccatc
    21361 tggaagtgga tgaaaatggt gctgctcgcg ttatcctgag tcaagaagtc ttgaaacaat
    21421 acaatgtaac cgatgctgaa actgcagcca ttgtcggtgc acctggacgc attgacagag
    21481 tgagtctctg gggaattttt gtcgaacagg ctgatggcca ctaccgagtt cgcttacgca
    21541 gtaaaatcca tcctatcaat gaaatcgcca aggagcatga tggtggaggc caccctctag
    21601 caagtggtgc taattcctat agcctagaag aaaatgaaat catctaccaa aagttaaaaa
    21661 acttgcttaa aaactgataa aatacttgcc aaacttttca gaatctgata gactagtatg
    21721 gtaacaatct atggctcgca aagagaccat ggcagaaagg aaatattgca aaatgaaaaa
    21781 agatatccat ccagaatatc gcccagttgt cttcatggac acaactactg gttaccaatt
    21841 ccttagcggt tcaacaaaac gctctaacga aacagttgag ttcgaaggcg aaacttaccc
    21901 attgatccgt gtggaaattt catcagactc acacccattc tacactggac gtcaaaagtt
    21961 cactcaagca gatggacgcg tggatcgttt caacaaaaaa tacggtctca aataatgata
    22021 agagaacagt ttcggctgtt cttttttgtt tcttgaaatc aactgctgtt ttcatgttcc
    22081 agactcatct gtaggttcga tttccatgct actaggcagg aaggaaatag ctgtttcaac
    22141 acgtccataa tgagctgtac tattgtcacg aaccatactt tcattgatgg tccaagtgga
    22201 attcattttc ttaaaggcct ctcgaacctt ttccaaatct ttggaggcaa tggcctgctc
    22261 taaggtttca aaacgaggac ttagacggtt taccaatttt tccttttccg catctaggtc
    22321 aacaggattt tgttcttttt caaaagctaa tagagctgag gaaatttcgg tgagtttggc
    22381 ttcagtaata tctccagata gagctaactt ttccttaaca atcttaccaa cctcagaatc
    22441 cttattttct actctttcga agtctgatgc catcttcgta acgagtttct gggtctcagt
    22501 ctgaccacca tttttaaccg ctttggatgc atctgtgatt ttcacaaaga aaaaactcaa
    22561 gctttcttgg gcacctgctg gggaaagaat taaaaatgac aaagctaaaa ctagtaacca
    22621 gcttttagcc ttccaataat ttctgaccaa tgtagcctcc tttctccaca tcaccaaagc
    22681 aagtaaaaag tccactacct atgtgagtga tgtattcatt catcttatca gtagcaccta
    22741 ggttagtttg aaccttaaca aagttatcag gatccttctg gaaagaaatg aagagcaatc
    22801 ccgtatcaaa ctgaccagtc ttttcatcaa tgccatctga gtaagagtaa gaacaacgca
    22861 aaagtggctt gtccacttac ttagccaaac gaacatgcga atcatctagc aaaaaactca
    22921 aattcacttc atcaaactca tttttttacc gaaaggggca ctactttcct tgtaacgacc
    22981 aaaggtattt tcttgttctt cgagaccagt gcggtcccag gtctctaaaa acatctgaat
    23041 ccgacgaact gccatataag aaccattttc catccagtcc ttactatcag cccagataac
    23101 gcggtcaaag tcttgctcct tggttggatt tgctgttcca tccttaaaac caaagaggtt
    23161 ccacggagtc tccattcgtc cccgatagct gcaaatccag actgactcca acgaaggggc
    23221 accgcatttc tacccttacg tatgagattg cgaataacgt gaaaggcaat ttgctcatca
    23281 tctgcacaag catgaatcac aatatctcca ccagtatatt tttcacgtaa ttgctcctta
    23341 ggaaatagag gtaaatccct gaacagtcga gggcgcttgt tttccaagtt catccttttc
    23401 aagaaacttg cagatacacc aaaagtcagc gtcaaacgat gaggattgag cccaactgtc
    23461 atccgtccaa tccttgaata gctggataat tttatctcta tccgtcgtat gcaggtccaa
    23521 gacaacaaaa tagatattct tttgcatggg cgtactattt ggtacatcaa gcgaacctta
    23581 tgaaaaggtc tactgatttt aaagcgcttt cttgtcttct tgaattgaga ttttattttt
    23641 agacgcgaca atggtttgag cgtctgcttt cctaagatgt tctggacgtg gcgtaatatt
    23701 tcgccagcca tggcgcttca acagttgata gaaggcatca cgtgtgtagg aacgacctaa
    23761 ctccttttta taagcctgaa ataaggcatc aattgtaaca aattctcctg cctctgcagc
    23821 cttcaaatga cgggcaagaa aggctttctc ttcctcaact gtcatatatg catggttacg
    23881 accaccacgt gtttcttgaa ggagagagtc gagtccgaac tcctcatatt tt
//
LOCUS       DAYYQN010000032        23421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYYQN010000032 DAYYQN010000000
VERSION     DAYYQN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.032"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(45..185)
                     /locus_tag="ACHBRQ_001207"
                     /pseudo
     CDS             complement(45..185)
                     /locus_tag="ACHBRQ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(198..578)
                     /locus_tag="ACHBRQ_001208"
     CDS             complement(198..578)
                     /locus_tag="ACHBRQ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592882.1"
                     /translation="MIDLYLSKNNHRNQLLLEFFQNYGIEVSCHSISEMTRDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(571..1020)
                     /locus_tag="ACHBRQ_001209"
     CDS             complement(571..1020)
                     /locus_tag="ACHBRQ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592883.1"
                     /translation="MKALNKESILYCDELETELHDAEMMQLDEQIFLMPNYPCEFEVT
                     FLDYYHKKHNYPLFYESYLQNIMEFLESQDIKNGADAFIDDNHNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKSINFSNLLDSLIVSEYQMEPNLWEVSHD"
     gene            complement(1004..2362)
                     /gene="dcm"
                     /locus_tag="ACHBRQ_001210"
     CDS             complement(1004..2362)
                     /gene="dcm"
                     /locus_tag="ACHBRQ_001210"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFM9592884.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARKSYKSIFQT
                     EGEIEFHDIRDVSDAEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQLLNSKDFGVPQNRE
                     RVFIIGHSRKRGTRLLFPFRREGQTTNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     AK"
     gene            complement(2461..3240)
                     /locus_tag="ACHBRQ_001211"
     CDS             complement(2461..3240)
                     /locus_tag="ACHBRQ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9592885.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKILKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVGNSNRPSVKKRLGQVENETAPVSHSAPRETEVSETKYSE
                     TDSLFIEDEEDRNTQPILKRKVEKVTKYDRDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
     gene            complement(3237..3410)
                     /locus_tag="ACHBRQ_001212"
     CDS             complement(3237..3410)
                     /locus_tag="ACHBRQ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592886.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLTLKELGGAL"
     gene            complement(3619..4980)
                     /gene="rlmD"
                     /locus_tag="ACHBRQ_001213"
     CDS             complement(3619..4980)
                     /gene="rlmD"
                     /locus_tag="ACHBRQ_001213"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9592887.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEEYLVQSPHRNQDLDLAYLRSGIADLGHLAYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMENPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDEVVVALRDLLRRYDLKSYDEKEQSGLIRNLVVRRGHYSGQIMVILVTTRPK
                     VFRVDQLIEQIIKQFPRIVSVMQNINDQNTNAIFGKEWRTLYGQDFITDQMLGNDYQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVECVVLLQR
                     KKG"
     gene            complement(5054..6028)
                     /locus_tag="ACHBRQ_001214"
     CDS             complement(5054..6028)
                     /locus_tag="ACHBRQ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFM9592888.1"
                     /translation="MRKKYFLTGAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSENQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKTGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEEGGNFLSSLWQAIVNFFKSFGS"
     gene            complement(6102..6716)
                     /gene="pmp23"
                     /locus_tag="ACHBRQ_001215"
     CDS             complement(6102..6716)
                     /gene="pmp23"
                     /locus_tag="ACHBRQ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HFM9592889.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGVQTLTDNLY
                     LAQKQGVDVWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVHLNLYIIKCFTLFSTSG"
     gene            complement(6716..7690)
                     /locus_tag="ACHBRQ_001216"
     CDS             complement(6716..7690)
                     /locus_tag="ACHBRQ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9592890.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIERV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAETFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNKVYQDAESVEFIQNDNGTYSILIKNIEDIQSK"
     gene            complement(7698..8954)
                     /gene="glyA"
                     /locus_tag="ACHBRQ_001217"
     CDS             complement(7698..8954)
                     /gene="glyA"
                     /locus_tag="ACHBRQ_001217"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9592891.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDTDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     AENEAVLEEVRSEVKVLTDAFPLYED"
     gene            complement(9016..9447)
                     /locus_tag="ACHBRQ_001218"
     CDS             complement(9016..9447)
                     /locus_tag="ACHBRQ_001218"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9592892.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(9449..10051)
                     /locus_tag="ACHBRQ_001219"
     CDS             complement(9449..10051)
                     /locus_tag="ACHBRQ_001219"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9592893.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(10035..10874)
                     /gene="prmC"
                     /locus_tag="ACHBRQ_001220"
     CDS             complement(10035..10874)
                     /gene="prmC"
                     /locus_tag="ACHBRQ_001220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9592894.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(10874..11953)
                     /gene="prfA"
                     /locus_tag="ACHBRQ_001221"
     CDS             complement(10874..11953)
                     /gene="prfA"
                     /locus_tag="ACHBRQ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9592895.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(11950..12540)
                     /locus_tag="ACHBRQ_001222"
     CDS             complement(11950..12540)
                     /locus_tag="ACHBRQ_001222"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9592896.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            12663..12845
                     /locus_tag="ACHBRQ_001223"
     CDS             12663..12845
                     /locus_tag="ACHBRQ_001223"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HFM9592897.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            12981..14354
                     /gene="mnmE"
                     /locus_tag="ACHBRQ_001224"
     CDS             12981..14354
                     /gene="mnmE"
                     /locus_tag="ACHBRQ_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9592898.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(14401..15336)
                     /gene="dapA"
                     /locus_tag="ACHBRQ_001225"
     CDS             complement(14401..15336)
                     /gene="dapA"
                     /locus_tag="ACHBRQ_001225"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9592899.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(15392..16468)
                     /locus_tag="ACHBRQ_001226"
     CDS             complement(15392..16468)
                     /locus_tag="ACHBRQ_001226"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9592900.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(17054..17126)
                     /locus_tag="ACHBRQ_001227"
     tRNA            complement(17054..17126)
                     /locus_tag="ACHBRQ_001227"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17091..17093),aa:Thr,seq:ggt)
     gene            complement(17226..17735)
                     /locus_tag="ACHBRQ_001228"
     CDS             complement(17226..17735)
                     /locus_tag="ACHBRQ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9592901.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(17733..17829)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            17858..18289
                     /locus_tag="ACHBRQ_001229"
     CDS             17858..18289
                     /locus_tag="ACHBRQ_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9592902.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            18371..18748
                     /gene="mscL"
                     /locus_tag="ACHBRQ_001230"
     CDS             18371..18748
                     /gene="mscL"
                     /locus_tag="ACHBRQ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9592903.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(18790..19884)
                     /gene="hemH"
                     /locus_tag="ACHBRQ_001231"
     CDS             complement(18790..19884)
                     /gene="hemH"
                     /locus_tag="ACHBRQ_001231"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFM9592904.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            20039..21262
                     /gene="pepT"
                     /locus_tag="ACHBRQ_001232"
     CDS             20039..21262
                     /gene="pepT"
                     /locus_tag="ACHBRQ_001232"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9592905.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(21298..21951)
                     /locus_tag="ACHBRQ_001233"
                     /pseudo
     CDS             complement(21298..21951)
                     /locus_tag="ACHBRQ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            21949..22113
                     /locus_tag="ACHBRQ_001234"
                     /pseudo
     CDS             21949..22113
                     /locus_tag="ACHBRQ_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(22384..23421)
                     /locus_tag="ACHBRQ_001235"
                     /pseudo
     CDS             complement(22384..23421)
                     /locus_tag="ACHBRQ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731453.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6778 a         4970 c         4295 g         7378 t
ORIGIN      
        1 ctttctaccc atggactttg aggcattcat tgttccatct tctagtggcg aatcttttga
       61 tacaaacgat tcaattcact tggatagtga aactctcccg caaacatttt tctggttaac
      121 tcaatccagc tgatatttct ttcagccaaa ataatggaca agttctccca aaatcgttca
      181 gccatattgc ttctccttta gttagataaa taatgtgttt gtgccatgta aatcaattgt
      241 ttcgtatctc ttggtaataa agccctagct tcctccaaat ttagatttgg ataaactcgc
      301 ttatttgaaa ccgcaagagg aagtctgatg gttagttcag gattttttaa tatcatttca
      361 atgaaatccg ttagtcttaa attgtcacgg ttcttaaatc gtaataaatt aggagataaa
      421 aactcaaaac aatctgaaga atagctcatc atctcaatta atttatccct tgtcatttca
      481 gaaatcgaat gacaggatac ctcaatgcca tagttttgga agaattctaa aagaagttga
      541 tttctatggt tatttttact tagatagaga tcaatcatgt gagacctccc aaagattcgg
      601 ttccatttga tattctgaga cgattaagga atctaataaa ttggagaagt taattgattt
      661 cttgtcttca tcataagctt ttacagttac ttgggttgta agtattccct cttttccctc
      721 ggctcgatag ccttgcccat ataaaacaaa aacaagatta tgattatcat ctataaaggc
      781 atcagctccg ttctttatat cctgactttc aaggaattcc ataatgtttt gaagatagga
      841 ttcgtaaaat agggggtaat tgtgtttttt atggtaataa tctaaaaatg tcacctcaaa
      901 ctcacatgga taattgggca tcaaaaatat ttgttcatcc aactgcatca tttctgcatc
      961 atgtaattct gtttctaatt catcacaata tagtattgac tctttattta gcgctttcat
     1021 ctttttcctc tatttctttt aatttttttg cgattgcggc aatcacagga acagttacac
     1081 tattaccagc ttgtttatag agctgactat tactagagac ttttctagca gcttcaaaag
     1141 cccaatcagg aaagccctgc agtcgaaaac actctttagg agtgattcgt cgtattctca
     1201 aacggtaaaa ttgtccatct attaaaacac cagctacatg gtaaacttgt ttatcttctc
     1261 cttcatagct agccactact actcccattt gaccactagt tgttaatgta ttcgctatac
     1321 cttttccaac tctaccacga cgatactgag aacttggtct ttctaaattg attgaatctc
     1381 caatctctgc ttgagcatac ccttttttag ttgcttcccg gacttttaga aattggattg
     1441 gttctggaat cagtattttg ggaattttat ctcctccttg catcgtagtc agtgttggag
     1501 ataagccctc gcttccatag actcgacctg tctccttaaa gctagtcggt aaatctccaa
     1561 caacgacaat accatgacga tcctgagtat ttaaagtaaa catcggctct tggttttcct
     1621 taaagcgtct cccattttgt ctcttgtcta atctgtctgg tgtcatacaa ggaatcgcaa
     1681 ccttgaatcc ttctcctttt ccacgaacta aggttggtgc aagaccttct gaataataga
     1741 ctttaccgct cattccactt tttgatggat tcaaattccc tagtgttttt aaagtctcag
     1801 agttagttgt ttgaccttct cgtctgaaag gaaataagag tctggtacct ctctttctag
     1861 aatgtccgat aataaacacc ctctctctgt tttggggaac gccaaaatcc ttactgttaa
     1921 gcaactgcca ctcaacatca aaccccaact catcaagtgt ggtaaggatt gtggtgaacg
     1981 tccgtccctt atcgtgattg agtaggcctt taacattttc aagaaaaaga aaacgtggtt
     2041 ggatttgttt ggccgcccga gcaatttcaa agaacaaagt tcctctagta tcttcaaatc
     2101 ccaatcgtct tcctgcgatt gaaaatgctt gacaagggaa tcccccacag atgatatcga
     2161 ctttccctct aagtttttta aattctgcat ctgaaacatc tcgtatgtca tgaaattcta
     2221 tttctccttc cgtttgaaaa atggacttat aagatttcct agcaaattta tcaatctcac
     2281 aaaatccaat acactcgtgt ccaacacttt ccattccaag tcgaaatcca cctatacctg
     2341 aaaataaatc aataaatctc atttcttgat cacctctttc ttatcgacta ccattgtcat
     2401 aactgtcact ccacttgaaa tgacaataag cataactaat atccactcga ttgacgacat
     2461 ttaaccacct ccttttctaa cacggttatt ccaaacagaa tacaggccat taaacacaaa
     2521 ttcagcaagt acttccgctc gtctaacaaa gcgaacattg tcaagagcat actgatagat
     2581 tctttcaaaa tcagtcatag caatttcact ggctgtttca gaaaaacctt ctcgtctaaa
     2641 ttgatcttgt accaatcccc aaatataatc tcgatcatat tttgtgacct tttctacttt
     2701 tcttttcaaa ataggttgag tattcctatc ctcctcatcc tcaataaata aagaatcagt
     2761 ctcactatat ttagtctcac taacttcagt ctctctaggg gctgaatgtg agactggggc
     2821 cgtttcattt tcaacctgcc ctagtctttt tttaacacta ggcctgtttg aattacctac
     2881 tggggtagaa gataattccc ctaaataaat cttattagct agtcttcctt tctcacttga
     2941 agactgttga acttcatcaa ttaagtcata ttctttaaga atttttttga tagacagtaa
     3001 ttttgacttc gagcaaccta acagcttcat cagtttagaa ttagaaaata ctaaataaac
     3061 cgctccttct tcatctatcc aaccacgact gagagataat tctaaacgat cttttaaaat
     3121 agaataagcc acctttactt ctagtttcat atccatatat ttctcatcct caaaaagaat
     3181 tttaggtaat ttgtaatacc gttctgaagt ttggtattga tttgcggtaa ttcgtttcat
     3241 agcgcacctc caagttcttt gagtgttaaa tctccgcttg attccaatcg aaccaagcca
     3301 cttttttcta attcagccat aagagagatg gcttcaacaa tatctattcc catttcacgt
     3361 actaaaaatg aaatgacaat atagcgtgat gattgtaatt cttttgtcat tttccctcct
     3421 gaaaataaaa aaaggagaac aaaatacaat tgttctctct ttagataaat atttattgtt
     3481 gtattcattt ttccaatgcg caaacgacat ctacaaatac atatacatct atgtgccttt
     3541 ttaggacttt tttgacgtca aaattgcctt tatctacaaa cacaaaatgc ttgtaaaata
     3601 gcttaaaatc aaggattttc accctttttt tcgttgtagc aacactacac actccacgtg
     3661 atgcgtttga ggaaatagat ccaccggctg gactttcttc aattcatatc ccaactcttg
     3721 gtatagttta atatcacgcg ccatggttgc gacattgcag gagatatagg cgatgcgatc
     3781 ggctcctgtt tgggcgcttg ctttgataaa gctttctgtc aagcccttgc gtggaggatc
     3841 aaccaagata acggttggtt gaatcccatc tttaagccaa ttcttcatag cattttcagc
     3901 tgtgtcacag acatagtggg cgtttgaaat attgttcagc tgagcatttt ttttactatt
     3961 ctcaaccgct tctggaatca gttcaacacc gtagacttct ttgacatgct tggcgactga
     4021 taaaccaatg gttccaatac cagaataagc atcaataacc acgtcatctt tttttaactc
     4081 tgcaaagtca atggctgttt gatagagttt ttcagccatt tcagtattga cttggtaaaa
     4141 agcgggtcca gcgatttggt agtcatttcc caacatctgg tcagtgataa agtcttgtcc
     4201 ataaagtgtg cgccattcct taccaaaaat agcattagtg ttctggtcat tgatattttg
     4261 cataacagac acaatccttg ggaactgctt gatgatttgt tcaatcaatt ggtcaacacg
     4321 aaaaactttg ggacgagttg ttaccaaaat aaccatgatt tgccctgaat agtgtccacg
     4381 acgcaccaca agatttcgaa tcaagccaga ttgttccttt tcatcataag atttcaggtc
     4441 ataacggcgg agcaagtcgc gtagggctac tactacctcg tcaatcacag gatcttggat
     4501 aaagaaatct tccaaaggca tgagatcgtg ggaattctta cggaaaaatc ctgtttccaa
     4561 gacaccatta acacgacgaa caggcacctg tgccttgttg cggtacttga ctggattttc
     4621 catgccaagt gtttcagcaa cctctacatc agcaatccca gcaatcttgt atagactgtc
     4681 cttgacttgc ttggttttaa atttgagctg ttctggatag gcaagatgac ctaaatccgc
     4741 gattcctgaa cgcaggtaag ccaaatctaa atcttgatta cggtgtggtg attgaacaag
     4801 gtattcttca accttcccaa aaccaatctt tttattaacc ttgaggacac gcataaggat
     4861 tttttcactc ggtaaagcat tctctacaaa aaagaccaaa ccatctacct tggccacccc
     4921 ggccccttcg tgagtcaagt caacaatttc aacttctaca atatcatttt tctttaacat
     4981 tctcttctct ttctattgac cgacaaaaaa gacggcaacg ttacggttac catctattat
     5041 accatgaaat ttcttaagaa ccaaaactct tgaagaagtt gacaatggct tgccagaggg
     5101 agcttaagaa gttaccacct tcttctagtg ccttatcagc atcaaagtta aggttaatat
     5161 ttttaaaact gtcgccagct tgtgaaacaa tactttgttt aaggtcattc agggttttag
     5221 tgaagtctgc attgctgagg atatcacttt ttgagagatt caaagcaaaa ttgatgatga
     5281 tattgatctg gtttcctgtt ataacctgat caagtttgta gttttttaag gtatcttcaa
     5341 cgattttacg gacatcttcc tccgtcagat ttcccttgct ttctttagct ttggcgagtc
     5401 ctgacttaat atctgctaga gcaacgttta atttattagc atcatagcca gttttgtcct
     5461 tgttttcagc atttatatct gacaaagcct tcaactcttc ttgagccaaa tctttgttgg
     5521 cttgcggcac cttggctcca ttagcctcta gcgaataata aatccctgct aaagcactct
     5581 cacctgtaac tggaataggg gctgctacag taattttggc atgttccact cccaaggtta
     5641 ctgctgcgtt tcggtacata tcctgcgtca ctttagtgat gttttctggt gtttcaatct
     5701 tgacctcaag tggcgacttg tcacctagtt tttgaatctt ggctgatgaa tacaattgta
     5761 agctagaatc attggcaaca ttcataatct tagaatagac atcaggcgtc atagtcttga
     5821 gttctttagt atctgttgag gcattgtagc ctagtttttt tagagtttga tttttttgat
     5881 tttcagatag ggaagaacct aggacatatt caggttggac ataggtttca tcgataactt
     5941 tttgcacatc tgttgctgca tggacgctat tcatagctgt tactgcccac aagaccgcag
     6001 caccggtcag aaagtatttc tttctcatag ggaatttcct cctttacctt tctagagtaa
     6061 tatatctctc ttaaagaaaa cttataataa aaacacctgg actagccaga tgtcgaaaag
     6121 agagtgaaac atttgatgat gtaaaggttg agatgaacct gcctcgaata ataatagttt
     6181 cctccattta catagagttc tgcaccgtgg aagatagaaa tggggtgaat ataactataa
     6241 gtctttccag tggtattacc aagcaaggga gcaacagtct cacgagagta ctgtttggcc
     6301 aaagccaagg tattttcctt gccattttgg gcgataaaat cgatataggc aggtccaaaa
     6361 ttataggctt gaacagctgt ccagacatct acaccctgct tctgcgccag ataaagatta
     6421 tctgtcagag tttgaacgcc ttgccgaatg ctagaggcat tatcattgat ggtattggtt
     6481 gagccacttg cagactcact agactgcatg acgtcgcctt cttttccttt tgtttcggta
     6541 taaatcatag cgagcacaag ctcttcgttt gctggggtgt ctttttcact caagatttct
     6601 cgcaccatgg gttgataggt cataacttgc ttaacatctt ggtgaacgcg gtaagcttta
     6661 tatccagcaa aaaggaagac tgctagtaca agcactcttc gaattcgttt aaacattatt
     6721 tactttggat atcctcgata tttttgatta agatagagta ggttccattg tcattttgga
     6781 taaactcaac agactcggcg tcttgataaa ctttattggg aacgatgagc tcaattccat
     6841 ttgacaagga gagtttttgg ttttcaaatt tcttaagctg acgactggca tcaatttcat
     6901 caaactgaac tggttctggt acggcttctt tgacttggtc aataaagctc aaacgagctg
     6961 tcagattgtt gtcaaaaagg tcattggcca atttttcagg tgacaactca ttgctttctt
     7021 ctaagttgtt gaaaatcgct gatttgacct tagattgaaa ttgaaaatca tctgtgttaa
     7081 aagtctcagc aattctctga gctgtttttt ccagttcttt gatggatttc ttgggtgaaa
     7141 tcttaggggc gacagcaaga agattatctg aaaaataatt caaaaaagtc ccgttgtact
     7201 tgattcgttt ttcaatcaga tgatacttgc gactctgaag attaaccacc aaggcctcat
     7261 cagcccctgt tccaaatcca ggcaggttat tctgagtcaa cttgattgga ttatcaactt
     7321 ctcctccgag gtgggtcaag gtctctcgca gggcaattcg taagaaagcg aaatgttcta
     7381 caccttcttt agaaaattgc acaaaaatca agtcattggt cttaagattt tctgaaatgc
     7441 tgaactcctc tttccagaga ttagccagcg ttactgatgt ctccaacaaa tcgtcagtga
     7501 tatgattgaa gaagggattt tcttcttcga aaatcccagt cttggcttca tctgaataca
     7561 cacgttcaat ttttttacgc aagtattctt cgatttttgg agtaatattg agaaacttat
     7621 ccgctaggaa cagctcggta tcatccggac tgaactgatg aataatagct ttcttaatat
     7681 aaatgtccat aaaagtttta gtcctcgtat agtgggaagg catctgtcaa cactttgact
     7741 tcacttctca cttcttctaa gacagcttca ttttctgcat tcttaagggt tttaatgatg
     7801 agttcagcca ctttgcgact ttcttcttca ccaaatccac gtgcagtaat ggctgctgct
     7861 ccgatacgaa taccacttgt cttgaatggt gacaaggttt cgtaagggat tgagttttta
     7921 tttagggtaa tattgacttc atccagcaag ttttgggcaa cttttccgtt ttctacaact
     7981 ttagtcacat ccactaggaa gaggtgattt tcagtcccac cagaaatgat acggaaatca
     8041 gtgtcttgca agaaaacatc tgccatagcc ttgctgttct tgataacatt agcagcatat
     8101 tccttgaagg ctggatctaa tacttctttg aaggaaactg ccttagccgc cacaacatgc
     8161 tctaaaggac cgccctgaat acctgggaaa atagctgaat tgattttttt agcaagttct
     8221 tcgtcattgg tcaaaatcaa accaccacga ggtccacgaa gggttttgtg ggtcgttgtt
     8281 gttgtgatat gagcgtatgg aactgggctt ggatgaaggc cagccgcaac caaaccagcg
     8341 atatgggcca tgtccaccat gagcttcgca ccgacagcat ctgcgatttc acggaatttt
     8401 gaaaaatcga taatttgaga atatgctgaa gcaccagcta caatcagttt tggttttact
     8461 tcttgggctt gtttcaagat agcatcaaag tctaagagtt ccgtttcagg atcaacacta
     8521 taagaaacaa agttgtaggt ttgaccagag aagctaacag gagccccatg agtcaaatga
     8581 ccacctgatg ccaaatccat tcccataacc gtatcacctg gctcaatcaa ggacatgtaa
     8641 gccgcacagt tagcttggct tcctgaatgt ggttggacat tggcaaattt agcaccgaaa
     8701 atttcttttg cgcgttcaat agcaagagtc tctacaacgt ctactacatc agttccacca
     8761 taataacggc gtcctgggta accctcggca tatttatttg tcaagataga cccttgagct
     8821 gccataacag ccttggaaac tacgttttcc gaagcaatta actcgatatt attttgttgg
     8881 cgttcttctt ctttggcaat agcattccag agatcagcat catatgcttt aaaatcatct
     8941 ttgtcaaaaa tcataggtct tctcctttat tgtgtgacta gtccattagt ttgattttac
     9001 aataagaaaa tcaaactaaa agatgcgaat aaaccgtttc tgcattttat cacaagtata
     9061 gccaactttt tcataaaatg catgagcacc cagacgatga ttggcagaat ttaagcggat
     9121 aaacccataa ccacatcttt ttgcttcttc ttccaaccct tgtagtaaac ttttaccaat
     9181 accttgacct tgcgcttgag gtgaaactgc taaagctaag atattaaatc ctgctttgga
     9241 atagagtgat tcgtaaactt cagcgtggac atatccaagt aagacatgat tagctgcatc
     9301 ctcatagcca agtaggaaat gatgggaatc ctgagacagt ctagctagtt ggctagccgt
     9361 ttcctctgga ctaaaagtat aacccaaagc ctcttggttg atgtcacata tcgctttcac
     9421 atctgtttct tgcaaatctc ttagcatttc aagcctcctc aaaagaaatc tctggcaacc
     9481 gagcaagaat atcttctcgt ttaattgcgc cttggcgtaa gattttcacc ttgtctccag
     9541 acaaatccac aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa
     9601 cctcttggtc aaaatccttc agaatttgtt caaaggttac accacttgcc tgacctgaga
     9661 tattggcaga cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt
     9721 gactgggcat ccgaaatcca atagttgcaa ggtcagaatt tacccaatag ggaactcggt
     9781 cattggcttc gagaataatg gtcaagggac ctggcaaaaa ggtctctaca agtttttgta
     9841 gataagttgg ctgattctta gaaaagtgca agatatcctc gaaagaggcg atattgagat
     9901 tgagtgcctt atctctagga cgacgtttga gttggtaaac atggtcaact gctttttcat
     9961 ctaaggcctt ggcaaaaaga ccataaacag tctctgtagg tagaacgaca gctccaccat
    10021 tttccaactc ttgtctaatc ctgtccatca tcaaccacaa ccatcctatt ttgaccaaat
    10081 tggtctttga gtgttcgaac ttttttttca ggaagatgtt ttctaaaaag ttcgggaaca
    10141 ctttgacctt gcttgtatcc aatttcaagg taaatcttac caccatcctt gagatagtct
    10201 tttgcatctt ccgcaattct acagtaaata gctaggccat cctcgtctgc aaagagagct
    10261 agatgaggct ccgaatgcaa aacattcaaa cctacctctg actcatcttc acgagagata
    10321 tagggtggat tggatacaat tatatcatat ttttcagaaa tttctgcaaa acaatcagat
    10381 tttttaaaaa atatattaag attttgatcg ctagcattct ctgatgcaag ctctaaggcc
    10441 tcttgagaaa catctgctgc tgtcactgac caatctggtc tattttttgc tagagcgaga
    10501 gcaatagctc cactacctgt tccaatatcc agaactgaga gattcgtctc aagattttca
    10561 gttagaataa gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc
    10621 acctttaaat gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga
    10681 gctgctagtt gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc
    10741 tgctggaggg caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa
    10801 gagaggcttt ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat
    10861 tgagctaatt tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca
    10921 aggcatccac aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca
    10981 agccgatacg gtggtctgtg acacggtttt gtgggaagtt ataagttcgg atccgttctg
    11041 aacggtcacc agtaccgatt gtcgacttac gctcagcgtc ttgttcatcc tgagcaatct
    11101 gagcaaagtg gtcagcgacg cgtgcacgga taatcttcat agccttctcg cggttcttct
    11161 gctgggtacg ttcttcctgc atctcaacct tgatattggt tggcaagtga acgatacgaa
    11221 cggcagtcgc aaccttattg acgttctgtc caccagcacc agaggcgtga tagatgtcga
    11281 cacgaaggtc ttttggatca atgtcgtatt caacctcttc aacttctggc ataacaagaa
    11341 ctgtcgctgt cgaagtatga acacggcctt ggctttctgt cacaggaaca cgttgcacac
    11401 ggtgggcacc tgattcatac ttaagcttag agtatacaga ctgacctgaa accatagcaa
    11461 ccacttcttt aaaaccaccg acaccattca tagaggcttc catgacttca aagcgccaac
    11521 cttgggcttc cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt
    11581 cgtctccacc agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct
    11641 ttggaaggag caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt
    11701 tgagttcttg cttggccatt tcttccaagt ccgcatctcc gcctgattcc ttaatcatct
    11761 cttcggcatc gacgatattt tgaaggactt gtttatactc acggtaggct attacggtgt
    11821 cacgattgga agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat
    11881 cagggtcact cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat
    11941 catagatgtt catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg
    12001 gcaaaccgag atataggttt cattaccacc aatctggatc tgttctccat cgtaaacggg
    12061 cagtccatcc tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt
    12121 cttgatttcg tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc
    12181 attgcgaaag tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac
    12241 acgagctagg tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaaaacaca
    12301 gtaaggtttt tctggtaggt ctcggatata gccaaagata tccgttgttt cctcaatcgc
    12361 aagggcaggg cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc
    12421 cagagccgag gtcataatca caacaccttt tccttgctcc tcgtagttat aggccacttt
    12481 gagaatctca atcgttttac cagagttcat ggtcccataa cgatagtaca actgtgccat
    12541 gtttcttgct tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct
    12601 taagctttaa acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta
    12661 ttatgccatt tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc
    12721 ttgctaagga agtaacggaa gcggttgttc gcaacactgg agcacctcaa tctgctgttc
    12781 atgtcatcat caacgacatg ccagaaggaa cttatttccc acaaggggaa atgcgcacca
    12841 aataagctag cttaagcaga attgcttagg ctttttcaat ctccaagtag cacccattga
    12901 agaaatagtc taaatttgtt acaatttgaa aagagacttg gaaaattcca agaaaagagc
    12961 aattaattaa aggaaacatt atgattacac gtgaatttga taccatcgct gctatctcta
    13021 ctccactagg tgaaggggct attggtattg tccgcctgag cggaacagac agttttgcta
    13081 ttgtgcaaaa gatttttaaa ggaaaagact tgaacaaggt tgccagccac actctcaact
    13141 acggtcacat tattgatcct ctgactggta aagtcatgga cgaggttatg gttggggcta
    13201 tgaagtctcc aaagaccttc actcgtgagg atattatcga gattaacacc cacggtggga
    13261 ttgcggtgac caatgaaatt ctccagctag ctattcgtga aggggctcgg ttggcagaac
    13321 ctggtgaatt taccaaacgt gcttttttaa acggtcgcgt agacttgaca caggcagagg
    13381 ctgtgatgga tatcatccgt gccaagactg acaaggccat gaacattgcg gtcaaacaat
    13441 tagacggctc cctttctgac ctcattaaca atacccgtca agaaatcctc aatacacttg
    13501 cccaagttga ggtcaatatc gactatcctg aatatgatga tgttgaggaa gctactactg
    13561 ccgttgtccg tgagaagact atggagtttg agcaattgct aaccaatctt cttagaacag
    13621 cccgtcgcgg caaaatcctt cgtgaaggaa tttcaacagc tatcatcgga cgtcccaacg
    13681 ttgggaaatc cagccttctc aacaacctct tgcgtgagga caaggctatc gtaacagata
    13741 tcgctgggac aacacgagat gtcatcgaag agtacgtcaa catcaatggt gtacctctca
    13801 aattgattga tacagccggt attcgtgaaa cagatgacat agtggaacaa atcggtgttg
    13861 agcgttcgaa aaaagccctc aaggaagccg acttggttct actagtgcta aatgccagtg
    13921 aacaactgac tgcgcaagac agacaacttc ttgaaattag ccaagatacc aatcgcatta
    13981 ttctacttaa taaaaccgac ctgccagaaa cgattgaaac ttcgaaacta cctgaagacg
    14041 ttatccgtat ttcagtcctt aaaaaccaaa acatcgacaa gattgaagag cgaatcaaca
    14101 acctcttctt tgaaaatgct ggcttggtcg agcaagatgc tacttacttg tcaaacgccc
    14161 gtcacatttc cctgattgaa aaagcagttg aaagcctaca agccgttaat caaggtcttg
    14221 agctggggat gccagttgat ttgcttcaag ttgacttgac tcgtacttgg gaaatcctcg
    14281 gagaaatcac tggggatgct gctccagatg aactcatcac ccaactcttt agccaattct
    14341 gtttaggaaa ataagaaaaa tccatgatcc ttcattcggt catggatttt attgtcttta
    14401 ttagtaatct ggtcttaaga cccctgttac agttgcctta gttgcttcgt agtcgccatc
    14461 tacgacaacc ttgataatgc gtttggcatc ttcttctggt gctggaacaa gaggtagacg
    14521 agtgggtcca gcttcaaatc ccatatagtt aagaattgcc ttaactggag caggacttgg
    14581 ataagagaag agagcattaa ccttaggaat gaatttacgc tgaattgctg cggctttctt
    14641 catatcgctt tctgcaatgg cagtaaacat ctcgtgcatt tcatccccat ttgtatgaga
    14701 ggcaacagaa ataaccccat ccgccccaag gttcatggca tggaaagcat ctccatcctc
    14761 acctgtataa atcaagaact cttcaggctt gtgctcaatc aagtaagcca tattagccaa
    14821 gctagtacat tctttgacac cgataatatt tggatggtca gccaagcgaa gcatggtttc
    14881 tggagtcaat tcgaccacta cacgccctgg aatgttatag ataataattg gtaggtcaga
    14941 agcatctgca atagccttaa agtgctgata catcccttct tgagaaggtt tgttgtagta
    15001 aggaacaata gcaagcccag ctgcgaaacc accaaattcc gctacttctt tgacaaactc
    15061 aatagagtca cgcgtatcat tagtacctac acccgcaatc aaaggaacgc gtccattgac
    15121 aaccttttgt acagccgcaa acaactccaa ctcctcatcg tgggtcaaag ttggactctc
    15181 agcagtcgtt cctgcgagaa gaattccatc cgtatgatgg gccaataaat gctcaatcaa
    15241 ggctggaata gcatcaaagt taatggaacc atcctcatgg aagggggtaa taaaggctgt
    15301 aatgatttta cattctttta aatcttgata agacacgaaa acacctctct atttcaaaga
    15361 aggagttcat ctgaactcct aaacgatatg actattttaa ttcaaatttc aattcagctg
    15421 ttggacgaac caatccacgt tcatgaagag tttcagcaat ctgaactgag ttccaagcag
    15481 cacctttgag aaggttatct gaaacaaccc acatgtgaat tcctttttct gcatccaagt
    15541 ctttacggat acgaccaaca aaggtatcac gtgaaccaac tgcattgatg gcttgaggat
    15601 agatttgatg aactacatca tcttcaagaa cagcacctgg gaaggatgcg atagctgctt
    15661 tgacttcttc aattggagcc acttcctttg tttcaatata aactgactca gagtgagctg
    15721 acaagactgg aatacgcaca catgtcgcag atactgcaat gctatcatct tccataattt
    15781 tcttagtttc cttggtcatc ttcatctctt cgtacgtgta atcattatca gtgaagacat
    15841 caatttgtgg aagagcgtta aaggcgatag gataatgttt cttgtcacca cctgaaggca
    15901 agatttccgc atgcaaatca cgtggtttca ctccatcatt caagacctca cgaagttcac
    15961 gttgtgtctc aagaattgct cccataccag cacctgaaac ggcttgataa gttgaaacaa
    16021 tgatacggtc caagccccat ttttggcgaa ccggctcaag agccaccatc atttggattg
    16081 ttgaacagtt agggcaggca atgattccgt tgtgggcatc aagtgcatga gcattgacct
    16141 ctggaacaac caaaggaaca tctggatttt gacggaaata agatgtatta tccactacta
    16201 ccacgccagc ttttactgcg tatggtgcat acttagctga tgtcgaacca cctgctgaaa
    16261 agagagcaat atcaactcct tcaaaagctg tttcagtcgt ttcttcaatc gtaatatctt
    16321 gatctttaaa tttcaatgac ttacctgctg aacgtgcaga agcaaggtaa cggattttat
    16381 cgattggaag tgttgattct tccaacattt ttatcatctg agcaccgaca gcacctgtcg
    16441 cgccgactac agcaactgta tatcccataa ataacctctt tcggaatttt ctaaaaattt
    16501 ctataataga agtattatac tactttttcc atgaattgca aagctttttc atcatttttt
    16561 ggaaaataaa aatccccaat cgctagacta gggattaaga ggcatcttca tgtgatgagc
    16621 aggttcacac aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta
    16681 gcccgaagac tacctatcga ctcatcgcat ctactattct actaaagaga atgacttttg
    16741 tcaacagacg aaactcttta tactcttcga aaatctcttc aaacttcgtc agcgtcgcct
    16801 tgccgtatgt atggttactg acttcgtcag tttcatctac aacctcaaaa acatgtttta
    16861 agctgacttc gtcagttcta tctacaacct caaagcagtg ctttgagcaa cctgcggcta
    16921 gtttcctagt ttactctttg attttcattg agtattagtt ttatttacac aggtgcataa
    16981 gaaatatatt ggaaaaattg ggggcagaca taacaaaaac ccttgtaaat caagggcttt
    17041 tctgtctatt taatgctaat tgccgggatt gaaccggcga cctcatcctt accatggatg
    17101 cgctctgcca actgagctaa atcagcttac ttaagaagta tactatattc agaaaaactt
    17161 gtcaagtttc cttagaaatt ttccatagga aaaagccagg taagaactac ctagcttatc
    17221 ttcttctatt tacttaaatc aattcgacga ccaattttac caataataat cgctccaata
    17281 atcaatgaag caaattcacc gattcctgtt gtaaaccaag taaggaagaa tggtaattct
    17341 gctacaatat ttaactctgc agcgatagta aacatggaaa ttgaaaagag gattgaaaag
    17401 aagaaatgat cttttcgaat caatccgtta aagaggtaat ccttgctata ttttgcaaaa
    17461 agccaaacac ctaaactgag gaatactaag gttgaaccac caccaacaaa gacatctagc
    17521 agtccgaagc taaagaaatt agcaatcata caaccgatgg taactccgat gatgtactta
    17581 ggattgtaaa aagccataaa gttcatcatt tctgaaatac gaaactgata agcaccatag
    17641 ctgatggcat ttaggggcgg tgtgacagtc aaaacgacgt agatagcagc gacgattgca
    17701 atatctgcaa catcacgaat agttaatttt ttcatctttt ctcctttggc ggttatccgc
    17761 gtgtaatatg cttggtgaaa gaagctaagc accaagggtt gattcattca accttactag
    17821 tatagcacaa tagcctgctt tatgctatac ttaaagcatg aaaaaaccta ttcaaaaatt
    17881 ttttaacaat gaaatcttag cctatctctt ttttggcctt gcgacaactc tagtttcgat
    17941 tctctcacga ctagtcattt atcaactaag tcatcaggaa ctccttgcta cggccttggc
    18001 aaatattatc ggtatcttat ttgcctttat cacaaatgat acgattgtat ttaagcaaaa
    18061 aaagaaaaat agacttatcc gtttagtgaa attttctctt gctcgccttt ctacttttct
    18121 gttagacttg tttttaactt ttctctttgt aacccaattt cctcacatca tagggcaatt
    18181 cgtaactgaa aacattgata agataaatgc tattgaaaca ataattgcac aaatattcat
    18241 tattatcatc aattatatct tgagcaaggt ctatattttt agaaaataat gctccattta
    18301 tgagcctttt aattgccttc tataatgatt taggatacaa tgaaacacga aacattgaaa
    18361 ggaaaacagt atgttaaaaa atctaaaatc gttcttgctt cgaggaaatg ttattgacct
    18421 tgctgtcggt gttgtcattg cctctgcttt tggtgctatc gttacttcac ttgtaaacga
    18481 cattatcact cctcttattt taaatcccgc tttgaaagct gctaaagtcg aacgtatcgc
    18541 tcaactttct tggcatggag tcggctatgg taacttttta agtgctatta tcaattttat
    18601 ctttgtgggt accgccctct tctttattat caagggcatt gaaaaagcac agaaactgac
    18661 tggcataaag aaagaaaaaa ctgccgaaaa aaaaccaacc gaattggaag tccttcaaga
    18721 aataaaagct ctccttgaga aaaaataatc gattaaaagg atctagcgca aagctaaatc
    18781 cttttttctt tactctttgg gtaacttgcc tgctttttca agcatttttt tgataagata
    18841 aggcatctta acattctccc gccctttttt ctcaatcagt tttttcacaa attctggcat
    18901 ttgtaaatct tcagattcag ctaaaatgtt tttcgtctcg tctgaaggaa ctaactcatc
    18961 aaaggtctct tcttctggga gaaattcctt aaactcttga ttttcattga ctctaactgt
    19021 attgaccaaa gcatcaatca aacgagaatc tgtatttggc attggtggac gatggtagtt
    19081 taccccaaat tcctggcata agtcataaca ttctacatca ttgtcaaaca agacctcaat
    19141 atgctcactg atgaaactaa taggtacaaa aatataatgg tctggatgtt ctgtctgttc
    19201 tctgagatac tccaaaacat ctggtttaat ccatggaata ccaatatcac tctcactctg
    19261 ccaagtgttg gtatattgtt ctgaactcaa gcccagtttt tcagcgacta gcttgctatt
    19321 ttcgaaaatt tggtctatat aaggatcacc aaagtccaag gcaaaaatgg gcacactatg
    19381 ggctgaaaag atgactttaa agctgtcttg ctttacttct tcttttaaaa tcttagcaat
    19441 ttcatctgcc caatagttta agagcgcttc ttcctgatac cagtccttaa tgaccaaaaa
    19501 ttgaatttgt ttgctttcta aaaatttttc atagcccatg acagagtaga aagaataatg
    19561 gggctccaaa atcaagcaaa tacactgttc aattccgtct gcttccattt gaccgatcac
    19621 atctggaata aagggactgg aaaacttatt agcaaagtag acactgtatt cattccccag
    19681 cctagcttct accaaggcaa cctcttgacg ggtaattttt tgcagaggcg tgcctccaat
    19741 tcgtacataa ttatcataga gagtctgaat ctcgtggtct tggggtctca ccccacgacg
    19801 aatatttgtg aaaaaatcag ctacaccttc aaaggtaatc tcttctggtg aaccgaaggt
    19861 catcattaaa attgcttttt tcatgactgc ttccttgtga tgtttttatc aagtttattt
    19921 tatcattaat taatcgataa gtaaacgcta atatagggac attctcaatc ttctgtcttt
    19981 tgtttttctc ttctttctat gatacaatgg aaaaaataaa ttcaaaagga gtttttttat
    20041 gacttatcca aatctcttgg accgcttctt aacctacgtt aaggtcaaca cgcgctctga
    20101 tgaacactct actactactc caagtacaca gagtcaggtt gacttcgcaa caaatgtcct
    20161 aattcctgaa atgaaacgtg ttggactgca aaatgtttac tatctaccga atggttttgc
    20221 tattggaacc ttgccagcca acgatccgtc tttaacacgt aagattggtt ttatatcgca
    20281 catggatact gctgatttta atgctgaagg agtcaatcca caggtaattg aaaactacga
    20341 tggtggtgtg attgaactag ggaattctgg tttcaaactc gatccagctg acttcaagag
    20401 tcttgaaaaa tatccaggac aaacgctcat cacaacagat ggaacaagct tgctaggtgc
    20461 tgatgacaag tcaggaattg ctgaaattat gacagccatt gaatatctaa ctgctcatcc
    20521 tgaaattaag cactgtgaga ttcgtgttgg ttttggtcca gatgaagaaa tcggtgttgg
    20581 tgccaataaa tttgatgcag aagattttga tgtggatttt gcctacactg ttgatggtgg
    20641 tccactaggt gaacttcagt acgagacttt ctcagccgct ggtgctgaat tgcatttcca
    20701 aggccgtaat gtccaccctg gtactgccaa agagcagatg gtcaatgccc ttcagctagc
    20761 aattgatttt cataatcaac ttccagaaaa tgaccgacct gagttaactg aaggttacca
    20821 aggtttttac catctaatgg atgtgacagg tagtgttgag gaggtgcgtg caagctacat
    20881 cattcgtgat tttgaaaaag atgcctttga agcgcgtaaa gcatccatgc aatctatcgc
    20941 tgataagatg aatgcagaac ttgggagcta ccgtgtcact ctcaacttga cagaccagta
    21001 ctacaatatg aaagaagtca ttgaaaaaga tatgactcca attaccattg ctaaagccgt
    21061 tatggaagat ctaggtatca cgcctattat cgaaccaatc cggggtggaa cagacggctc
    21121 taagatttcc tttatgggaa tcccaactcc gaatatcttt gcaggtggcg aaaatatgca
    21181 cggacgtttt gaatacgtta gccttcagac tatggaacgt gcagttgata ccatcattgg
    21241 catcgtagct tataaaggct aaaaagacga ggtagctcag ctacttcgcc tttcttttta
    21301 ttctactggt ttttcttgat ttccagtagt tgtagaagat tctgttgttt cattttctga
    21361 agttgattca gcaggtttag aatctcttgt attgcttggt ttgttttcgt cgctagcaat
    21421 ttcaatgtta gattctgcag ttgcgtttgg ttggttctca gcactggtgt tatcactatt
    21481 tgcttcagca tttcttgctg gacttgtttc ttcacttgcg ctagcttttg actggatttg
    21541 atgattcaaa actagaatag cttttgtcga ttcaagtaaa gctgttttgt ctttactctt
    21601 agcagaaagt tgatctaata atgcatccac cttatcaaag tccgcatcag atccattatt
    21661 actttctaaa taagagtgaa gcgacatgag aatatcgtag agtttttgat agagtacaag
    21721 tgtctgagaa tcttgctcag cattttcctt ttcttgttga agggcgctag cgatacgagt
    21781 caagacatct tttacctgac tgtttacttc atccaagtct gcatcagcct tgtttgtggc
    21841 agcttttaga ttttctactt cttctgccaa ggattgtctg attccttctt catggatttg
    21901 ttccaagagt tgatttacct tgctcaaaag actttctact tcttccttgc tatctgtcgc
    21961 agattattgg ttgccatcta ccatgtactc ctaaaacagg agagttataa tccaagatta
    22021 caaggcctta cagaaataag aaatccagat aagacaatgt tcgtccaaga cgctattcgc
    22081 ttcgcacagc agcacggatt caatatgctt taattttaaa gtttaggtgt caagacctct
    22141 ttttagtgtg cccaaaattt agagaagtaa tcaatcaact aacttttatt tttttcaaac
    22201 tttcagtaaa ctgacctaaa gctaactcaa tctgtctttg tagatgcttc tgctatcagc
    22261 tagaagttga tctacttttg ccaagactgc cttctcatca aaagttccag gttgatagtt
    22321 ggattgcagg gatggaatct tgtttttcaa agccgcttca tatcccttag tttgaacctt
    22381 gatgtagtga ttgtggtcgc catgaggaat cacaaaacct tctgaatctt cacttataat
    22441 tcgattggca tcaaaaccat gaccatcttc ttcctcatgg tggacatgta gtgacggatt
    22501 acttaataca gaactagaag aacttcctgc ctcttccgtg ttagagtgtg atgggggatt
    22561 gttaagagat gacttaggaa tataatgata gtgatccccg tgtcttacta tataagcatc
    22621 acctgtatct ctgacaatat cattagggtt aaagacataa ccatcatctg ctgcagaaac
    22681 accattattc ggtgtcaccg acaaagattg actgagagct gtagtattcc ctgataatta
    22741 tacttttgca gctgctaatt cacctgccga caagtcactc tcaggaatga aatgatagtg
    22801 accaccatgt ggtactatag taggttgaaa tagaatatga gcaaattgat aaggggattt
    22861 taaagtaatt tctaacaatg atttagaaac tatgatgtgc tattctaaat tcaactcact
    22921 atatataacc atcatcggta gtataacgtc cctgtaattt tgctacagat acttctgcac
    22981 tagctccttt atcgtcttta ccatgttctt gtttttggcg attgatttca tcttttgttc
    23041 gtacattttc tgcatgagct tgatctttaa ggtaaacata atactttcca tctaccttaa
    23101 taatatatcc tcccttaacc taactgacga tatcttgatc tttcggctga tagttggggg
    23161 ctttcattaa tagctcttca ctaaagagcg catcaaaagg aactttacca ttatagtagc
    23221 gataatgatc gccatgagaa gttacataac cttgatctgt aatcttaata acaatttgtt
    23281 ttgcttgaat tccttctttt tgactaacct agtctggagt caaattttca gtcttcttag
    23341 tgtctttatt actgtttaca tatgaaacac gatttttatc tgtattggcc tgttagctat
    23401 gttggttcag agtataaaca c
//
LOCUS       DAYYQN010000033        22498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYYQN010000033 DAYYQN010000000
VERSION     DAYYQN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.033"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            183..452
                     /gene="blpJ"
                     /locus_tag="ACHBRQ_001236"
     CDS             183..452
                     /gene="blpJ"
                     /locus_tag="ACHBRQ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HFM9592906.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            521..769
                     /gene="blpK"
                     /locus_tag="ACHBRQ_001237"
     CDS             521..769
                     /gene="blpK"
                     /locus_tag="ACHBRQ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HFM9592907.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            1013..1162
                     /gene="blpO"
                     /locus_tag="ACHBRQ_001238"
     CDS             1013..1162
                     /gene="blpO"
                     /locus_tag="ACHBRQ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HFM9592908.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1266..1385
                     /locus_tag="ACHBRQ_001239"
     CDS             1266..1385
                     /locus_tag="ACHBRQ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HFM9592909.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            1905..2224
                     /locus_tag="ACHBRQ_001240"
                     /pseudo
     CDS             1905..2224
                     /locus_tag="ACHBRQ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            2853..3236
                     /locus_tag="ACHBRQ_001241"
     CDS             2853..3236
                     /locus_tag="ACHBRQ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HFM9592910.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            3288..3977
                     /locus_tag="ACHBRQ_001242"
     CDS             3288..3977
                     /locus_tag="ACHBRQ_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9592911.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            4019..4252
                     /gene="blpZ"
                     /locus_tag="ACHBRQ_001243"
     CDS             4019..4252
                     /gene="blpZ"
                     /locus_tag="ACHBRQ_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HFM9592912.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            4403..5014
                     /locus_tag="ACHBRQ_001244"
     CDS             4403..5014
                     /locus_tag="ACHBRQ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9592913.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            5175..5969
                     /gene="ccrZ"
                     /locus_tag="ACHBRQ_001245"
     CDS             5175..5969
                     /gene="ccrZ"
                     /locus_tag="ACHBRQ_001245"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HFM9592914.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5966..6601
                     /gene="trmB"
                     /locus_tag="ACHBRQ_001246"
     CDS             5966..6601
                     /gene="trmB"
                     /locus_tag="ACHBRQ_001246"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9592915.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6727..7206
                     /gene="rimP"
                     /locus_tag="ACHBRQ_001247"
     CDS             6727..7206
                     /gene="rimP"
                     /locus_tag="ACHBRQ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9592916.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            7250..8386
                     /gene="nusA"
                     /locus_tag="ACHBRQ_001248"
     CDS             7250..8386
                     /gene="nusA"
                     /locus_tag="ACHBRQ_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9592917.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            8408..8701
                     /locus_tag="ACHBRQ_001249"
     CDS             8408..8701
                     /locus_tag="ACHBRQ_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9592918.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8694..8993
                     /locus_tag="ACHBRQ_001250"
     CDS             8694..8993
                     /locus_tag="ACHBRQ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9592919.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            9010..11802
                     /gene="infB"
                     /locus_tag="ACHBRQ_001251"
     CDS             9010..11802
                     /gene="infB"
                     /locus_tag="ACHBRQ_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9592920.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            12053..12403
                     /gene="rbfA"
                     /locus_tag="ACHBRQ_001252"
     CDS             12053..12403
                     /gene="rbfA"
                     /locus_tag="ACHBRQ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9592921.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            12682..13461
                     /locus_tag="ACHBRQ_001253"
     CDS             12682..13461
                     /locus_tag="ACHBRQ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592922.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            13463..13645
                     /locus_tag="ACHBRQ_001254"
     CDS             13463..13645
                     /locus_tag="ACHBRQ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592923.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(13923..14165)
                     /locus_tag="ACHBRQ_001255"
     CDS             complement(13923..14165)
                     /locus_tag="ACHBRQ_001255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592924.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEVSSKHRFEVVDRTDEVS
                     SKHRFEVVDRTDEVSSKHRFEVVDRTDEVSNIYTAR"
     gene            14196..14429
                     /locus_tag="ACHBRQ_001256"
     CDS             14196..14429
                     /locus_tag="ACHBRQ_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HFM9592925.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            14429..15763
                     /locus_tag="ACHBRQ_001257"
     CDS             14429..15763
                     /locus_tag="ACHBRQ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HFM9592926.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            15773..16027
                     /locus_tag="ACHBRQ_001258"
     CDS             15773..16027
                     /locus_tag="ACHBRQ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HFM9592927.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            16123..16518
                     /locus_tag="ACHBRQ_001259"
     CDS             16123..16518
                     /locus_tag="ACHBRQ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9592928.1"
                     /translation="MSKKLNRKKQLRNGLRRSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            16930..17379
                     /locus_tag="ACHBRQ_001260"
     CDS             16930..17379
                     /locus_tag="ACHBRQ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HFM9592929.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            17366..17923
                     /locus_tag="ACHBRQ_001261"
     CDS             17366..17923
                     /locus_tag="ACHBRQ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9592930.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            17925..18887
                     /locus_tag="ACHBRQ_001262"
     CDS             17925..18887
                     /locus_tag="ACHBRQ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HFM9592931.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSEHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            18899..21550
                     /locus_tag="ACHBRQ_001263"
     CDS             18899..21550
                     /locus_tag="ACHBRQ_001263"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9592932.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAINPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTINPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            21620..22375
                     /locus_tag="ACHBRQ_001264"
     CDS             21620..22375
                     /locus_tag="ACHBRQ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9592933.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
BASE COUNT         6870 a         3969 c         5028 g         6631 t
ORIGIN      
        1 agatatttta gatactcaag attattgaaa tcttagacta aaaaataggt actgctacct
       61 ttaagaagat agtagacgta tatacttttt taagaaaatc aaaaagatac taaaaaaaat
      121 ctatttgttt attgaattta agactttggt aacaaattga aaataaaaag gaggtattca
      181 tcatgaatac aaaaatgttg tcacagttag aggttatgga tactgaaatg cttgcgaaag
      241 ttgaaggggg gtatagctcc actgattgtc aaaatgcact gattacagga gtcactacgg
      301 gaattataac tggtggaact ggagcaggtt tggccacttt aggagtagct ggacttgctg
      361 gtgcatttgt tggagcacac attggtgcaa ttgggggtgg attaacatgc ttaggaggta
      421 tggttggtga taaattggga ctaagttggt aagacttata gtaaaattat gaggattcta
      481 ttctgattta ttttagtttt ttaaaaaagg agtatttgtc atggatacaa aaatgatgtc
      541 acaattttct gttatggata ctgaaatgct tgcttgcgtt gaaggtggcg gatgcaattg
      601 gggagatttt gccaaagcag gtgttggagg agcagctgta gttgctgctt taggttgtgc
      661 agcgggtgga gtgaaatatg ggaaaattct aggaccatgg ggcgctgcaa taggaggaat
      721 tggaggagca gtggtttgtg gatatttagc ctataccgct acatcataaa aatgaaagct
      781 aaattttatg aaggctatat tctttattat tctcttcgct tttcaaacct atcttattta
      841 tctatccata agtatctctg ataaaaaaca aaagactatt gaattaacat cattgaattg
      901 ttttgtcata ttatttctaa tctatgataa attaattttt ctctttattg cctatgtttt
      961 tttgataatt tttatattaa acttgttccg taactgaaaa gtgacatact atatgaatac
     1021 aaaaatgatg tcacaatttt ctgttatgga taatgaaatg cttgcttgcg ttgaaggtgg
     1081 agatattgat tggggaagaa aaattagttg tgcagcaggg gttgcatatg gcgcaattga
     1141 tgggtgtgca acaacggttt gacatttcta ttgggaccat ttgctatagg aataggtgta
     1201 actggtgctg caggtggagc tatacttgga ggtgtagcct atgcagcaac atgttggtgg
     1261 taattatgga ttttaaaagt tttattattg gtttagtagt tggtatattt ggtccttata
     1321 tggatgactt aattagaaaa atattttcaa aatcttccaa aaaggatacg gatagtactc
     1381 tctaaaaaat aaatttctcc cctgtatttg ctagcttaat ttacagggga gtttctttaa
     1441 tattgtttcg cctgtcaaaa tgatatgtga tagcaggatg aaaagtctac aaacaagttg
     1501 gtatcaatac aatataaaga ggaaagtatg tttttcatgc ttgctttttt aatttttaca
     1561 attcaagagg ttttgatgac catttatgat ttgagtgatc caaggtcaaa ataagtgcta
     1621 tactagcagt gtaaatgttc ttgctcaact aagataaatc gcacatttag gaggaaaact
     1681 tatgaagaaa aaaatactga ttattttcgt tctgtatctg atcatgtcca tctttcttta
     1741 tccgcttagg gagagtattt ggtataatct attttatacc atagcctata tgattgcggt
     1801 tatgatctat ttttctttaa ttaaaaagaa agaaaagaaa tgaaagattt tcttgattaa
     1861 aaagaatcat attctttgtg agattgttcc tgggtcagtt gcctttgctt gtttctactt
     1921 atctatttct atttcgtcag ttttccaatt tcttttagta gttattaact tgacttctat
     1981 tttggttact gtttatctta ctagggaaat gagattaaga gagtttgaag atgatgattt
     2041 ggttagtcct agaaccaatc aactcatgtt tatcggcttg acaggcttta tgtctattat
     2101 ttgtttgtat agaggtatca cagcaggaga atcttatcaa caactaatcg cttatattgg
     2161 tgctattctt tgcttgatca tcatgcttct actcatttgg ggtttgaagt attataaaaa
     2221 gtaggggcta atgaagtttt tgcaatttag gaggtttcga tgacaattca agatttggat
     2281 gaaaaatttt aaaaaacaat gatatactaa acttgtcaaa gttgcaacgg gtgctgctac
     2341 attatgttaa aaatcgctct tgattgataa gttggatttg ttaggaaagg agtaaataat
     2401 tatggataag aaaaagatag tttcgactat aatatgtata gtatttcttg tagtgtcagt
     2461 agataatttt tttagagact taaccccgtt actgttcatt ttaaatatta ttggcctgtc
     2521 ttgcttttca gtgctaacat acattaacat aaaagagata ctcttaaata taagtaaatg
     2581 atgatttggt tagtcctaga accaatcaac ttatgtttat cggcttggca gattttatgt
     2641 ttgtcatttg tttgtataga ggtatttcag aaacagaatt ctatcaacaa ctaatcgctt
     2701 atataggtgt attctctgct tgtttatcac gcttctgctc atgtggggct tgaagtatta
     2761 taaaaagtag ctttctatcc ttttctgaca ggatatttga gtagaaggct ctattaaatt
     2821 gttagtgaaa taaatgaatg gaggtattta atatgaaata cagattattt tttgttattt
     2881 tcttgagtag tgtgttggat attcttttag ggacattttt acaaatctct atcgtatcta
     2941 ttgggtggct tgttctttac agtggtttgt ttgaagcagg agttttcctt cttgctaata
     3001 aaggggtggc ggtaaaaatc aaggaagtag atattcgaaa tcgctttaaa tttatttttg
     3061 gaaaaacctt atggtttcaa attcttttgc tcatcttttt gataatcaaa ctttatcttg
     3121 gtttggatgc gaggttgatt ttattctatg gacatatttt cattgtcttt aatgccttaa
     3181 tgtatctatt atctagtagt caggttagcc ttaaaaaaaa caaactgtct tcttaatctt
     3241 acagcagtag agcctgacaa ggtgaggtca tagtagggag gatttctatg aaaaagtatc
     3301 aacttctatt caaaataagt gcagttttct cttacttatt tttcgtattt ggtctttctc
     3361 agatgacgct tattattcaa aattattggc aattttcttc ccagattggc aatttcgtct
     3421 ggattcaaaa tttcttgagt ttgctattta gcggagtcat gatttggatt ctggttaaga
     3481 caggtcatgg ttatctcttt cacattccaa gaaaaaaatg gctttggtat tcgattttga
     3541 cagtattagt ggtagtgctc cagatctctt ttaacgttca gacagctaaa catgttcagt
     3601 caactgctga aggttgggct gtattgatcg gttatagtgg gaccaacttt gctgagctag
     3661 gtatctatat aactttgttc tttctgactc cacttatgga agagctaatc tatagaggat
     3721 tactgcaaca cgcctttttt aagcattcga gatttggcct tgatttgctt cttccgtcaa
     3781 ttttatttgc tcttcctcat ttttcaagcc tgcctagttt gttagatatc ttcatctttg
     3841 caacatctgg catcatcttt gctagtttga cccgctatac caagagcatt tatccttcct
     3901 atgcggtgca tgtgatcaat aatattttcg caacattacc atttttgctg acttttttac
     3961 atagggtctt ggggtaaaaa aacaaaaggc ttgcttttca gccatagagg agattatcat
     4021 gtataaacac ttatttttcc tagattccaa aactttagat cggttgacac cctatattct
     4081 agtcttggct tctgacacca ttgcttttaa tgtttttgtg ctaacctttg tatctgcggt
     4141 ggtttttaat ttcctaaatt ccatgctagc tttaatggct atattcatag gggctggcta
     4201 tgtggtcgga ttttggttac taatactcaa tgaaaatcaa agagcaaact aggaaactag
     4261 ccgcaggctg tacttgagta cggcaaggcg acgttgacgt ggtttgaatt tgattttcga
     4321 agagtataaa atggtttgtt ttggaaaggc tagaactaaa gaatgacgcg taggaagtct
     4381 gtttggtgag gaggatagtt ttatggagtt ttttgataaa tttcatgcct tgtgttttgg
     4441 atttttagta ctaataattg tcattacagt tccttatacg attaaccatg ggggtttttt
     4501 tcaaaatgaa tctgcattga ttcttgtaag tcttcttgta acctcgctga gtgttgctta
     4561 tgctagaaag tttgaaatga tttcttttgg gatgttaagc aagaaacaac ttttgctttt
     4621 cattgcaatc tttcttctaa gtgtacttga gacgctggtt tatattcatt tcttcgctgt
     4681 ttcttctggc tcaggggtcc aacacttggc ggaagtcagc agaggaattt ccctgtcttt
     4741 gattttgact acctcagttt ttggccccat ccaggaggaa ctcattttca gaggacttct
     4801 tcaaggtgcg gtttttgaca attcttggtt agggcttgtg ctaacttcct ctctcttttc
     4861 tttcatgcat ggaccttcta atgtcccttc gtttattttt tatctacttg ggggtttgtt
     4921 gctgggcttt gcttataaaa agagtcaaaa cctatgggtt tctactctag tccacatgtt
     4981 ttacaacagt tggccactcc tatattattt ataaaaatta tgaaaaggta agcagaagat
     5041 ggtgcttacc tttttcaagc tataatgata cccaagccaa tccagaggct tttctttgag
     5101 aatcgggtta gagtggttga cgaataggcc aaaaactagt agaatagtaa ggaaacttta
     5161 tacggaggaa agaaatggat ttgggtgata atgagctaac actgactccc atacctggga
     5221 aaagtggcaa ggcttatatg ggtagctatc ctgacgggaa gcgcatcttt gtaaaaatga
     5281 acacctctcc aatcctacct ggtctagcta gagaacaaat tgctccacaa ttattatgga
     5341 gtcgtcgttt ggcagatggg cgtgatatgt gtgctcaaga atggttgaca ggcaagatat
     5401 tgacccccta tgatatgaat cgtaagcaaa tcgtcaatat tttaacccgt cttcatcgct
     5461 cacgtccgtt gatgacacaa ttgagtcgtt tgggctatgc catggaaaca cctgtagatt
     5521 tactacagtc ttggcaggaa acggctccag atgctttgcg taaaaatcat tttatcagtg
     5581 aagtgatggc tgatttacgt cagactattc caggatttag agaggaccat gcgaccattg
     5641 tccatggaga tgtacgacat agtaattgga ttgagacaga tagtggcttg atttatttag
     5701 tagattggga ttcggttcgc ttgaccgatc gcatgtttga tgtggcccat atgctctgcc
     5761 attatatttc agaacatcag tggaaggaat ggttgaccta ctacggttac aagtacaatc
     5821 aaacggtatt aagtaaattg tattggtatg gtcaattgtc ttatttgagt cagatttcca
     5881 agtattatat gaaccaagat ttagaaaatg tcaatcggga gattcatggt ttgcgtcatt
     5941 tccgagacaa gtatggaaag agaagatgag agttagaaat cgtaaagggg caacagaatt
     6001 actagaggca aatccccagt atgtggtcct caatcccttg gaagccaagg caaaatggcg
     6061 ggacttgttt ggcaatgata atcccattca tgtggaagtt ggaagtggaa agggtgcctt
     6121 tgtttcaggt atggccaagc aaaaccctga catcaactat atcgggattg atattcaaaa
     6181 gtctgttttg agctacgctt tggacaaggt gcttgaagtt ggagtgccta acatcaagct
     6241 cttgtgggta gatggttctg acttaactga ctattttgaa gacggtgaga ttgatcgctt
     6301 gtatctgaac ttttcagatc catggccgaa aaaacgccat gaaaagcgtc gtttgaccta
     6361 caagaccttc ttggatacct tcaaacgtat cttgcctgaa aatggagaaa ttcatttcaa
     6421 gacggataac cgtggcttgt ttgagtacag tttagtgagc ttttctcaat atggcatgaa
     6481 actcaatggt gtctggttag atttgcatgc cagtgatttt gaaggtaatg tcatgacaga
     6541 atacgagcaa aaattctcaa acaaggggca agttatctac cgagttgagg cagaatttta
     6601 agagatagcc taaaattagg ctgtacaagt gcttttgctt tacataagtt ggcaaacgtg
     6661 ctatactgat agtaagaata tgaaaagtga ggcggggaaa tatcttcgcc tcttgcttat
     6721 gaggaggtgg acgcaatcgc aacaatcgta gaattagtca gagaagttgt agaacctgtc
     6781 atagaagctc cttttgaact cgtggatatc gagtatggaa agattggcag tgacatgatt
     6841 ctcagtattt ttgtagataa acccgaagga attaccttga acgacacggc agacttgaca
     6901 gaaattatca gtcctgtcct agacaccatc aagccagatc ccttcccaga acaatatttc
     6961 ctagaaatta ccagtccagg tttggaacgc cctttgaaaa ccaaggatgc cgtcgctgga
     7021 gcggttggaa aatacatcca tgtcgggctc taccaagcca tcgataagca aaaggtcttt
     7081 gaaggaacct tgttggcctt cgaagaggac gagttgacta tggaatatat ggacaagacg
     7141 cgtaagaaaa ccgtccaaat tccatacagt ttagtatcaa aagcacgttt agcagttaaa
     7201 ttatagaaaa agaaaggata gcttttgagg attcaaaagt gaagaaaaca tgagtaaaga
     7261 aatgctagag gccttccgca ttttggaaga agacaaggga atcaaaaaag aagatatcat
     7321 cgacgcagta gtagagtcgc ttcgttccgc ttatcgcaga cgctatggtc agtcagacag
     7381 cgtagctatt gacttcaacg aaaaaacagg tgactttaca gtttatactg tccgtgaagt
     7441 tgttgatgaa gtatttgata gccgtttgga aatcagcttg aaagatgctc ttgccattaa
     7501 ttcagcttat gaacttggag acaaaatcaa gtttgaagaa gcaccagctg agtttggtcg
     7561 tgtagcagcc cagtctgcca aacaaaccat catggaaaaa atgcgcaagc aaacacgtgc
     7621 catcacttac aatacttaca aagaacatga gcaagaaatc atgtctggta cagtagaacg
     7681 ctttgacaac cgctttatct atgtcaacct tggtagcatc gaagcccaat tgtcaaaaca
     7741 agaccaaatt cctggagaag tttttgcttc tcatgatcgt atcgaagttt atgtttacaa
     7801 ggttgaagac aaccctcgtg gtgtgaatgt ctttgttagc cgtagtcatc cagaaatgat
     7861 caaacgttta atggagcaag aaattccaga agtttatgat ggaactgttg aaatcatgag
     7921 cgtggctcgt gaagcaggtg accgtacgaa ggttgctgtt cgtagccaca atccaaacgt
     7981 ggatgctatc ggtacaatcg ttggacgtgg tggtgctaat atcaagaaga ttactagcaa
     8041 attccaccca gctcgttacg atgctaaaaa tgaccgcatg gtaccaatcg aagaaaatat
     8101 cgatgttatc gagtgggtag cagatccagc tgaatttatc tacaatgcca tcgctcctgc
     8161 tgaggttgac caagttatct ttgatgaaaa cgacagcaaa cgtgccttgg tggttgttcc
     8221 agataacaag ctttctcttg ccattggtcg tcgtggacaa aacgtgcgct tggcggctca
     8281 cttgactggt taccgtatcg atatcaagtc tgctagcgaa tttgaagcca tggaagacgc
     8341 tgcttcagta gagttggaag tagaaaacga tactgtagaa gaataaaagc tgctagagga
     8401 gggaaagatg aaaacaagaa aaatcccttt gcgcaagtct gttgtgtcta acgaagtgat
     8461 tgataagcgt gatttgctcc gcattgtcaa gaacaaggaa ggacaagtct ttattgatcc
     8521 tacgggcaag gccaatggcc gcggcgctta tatcaaacta gacaatgcag aagccctaga
     8581 ggcgaaaaag aagaaggtct ttaaccgcag ctttagcatg gaagtggaag aaagctttta
     8641 tgacgagttg atcgcttatg tggatcacaa agtgaaaaga agagagttgg gacttgaata
     8701 agcaaaagat aagtaatctc ttggggcttg ctcagcgagc agggcgcatc atatcgggtg
     8761 aagaattggt ggtcaaggcc attcaagacg gcaaggccaa gttggtcttt ctagctcatg
     8821 atgctggacc caatctgacc aagaagattc aagataaaag tcattattat caagtagaaa
     8881 ttgtaaccgt gttttcaaca ctggaattaa gcatagcagt cgggaaatcg agaaaggttt
     8941 tggctgtaac agatgctgga tttacaaaga aaatgaggtc tcttatggaa tagaagagga
     9001 ggacatgatt tgtctaagaa aagattgtac gaaatcgcaa aagaacttgg aaaagaaagt
     9061 aaagaagttg tagcgcgtgc aaaagagttg ggcttggatg tgaaaagcca ctcatcaagt
     9121 gtggaagaag ctgtcgctgc aaaaattgct gccagcttta agcctgcagc tgctccgaaa
     9181 gtagaagcaa aacctgcagc accaaaagta agtgcagaaa agaaagccga aaaatctgag
     9241 ccagctaaac cagctgtagc taaggaagag gcaaaaccgg ctgagccagt tgctccgaaa
     9301 acagaaaaag tagcagtgaa accgcaaagt cgtaatttca aggctgagcg tgaagcccgt
     9361 gctaaagagc aggcagaacg acgcaagcaa aataagggca ataaccgtga ccaacaacaa
     9421 aacggaaacc gtcagaaaaa cgacggccgt aatggtggaa aacaaggtca aagcaaccgc
     9481 gacaatcgtc gctttaatga ccaagctaag aagcagcaag gtcagcaaaa acgtagaaat
     9541 gagcgccgtc agcaagagga taaacgttca aatcaagcgg ctccacgtat tgactttaaa
     9601 gcccgtgcag cagccctaaa agcagagcaa aatgcagagt acgctcgttc aagtgaggaa
     9661 cgcttcaagc agtatcaggc tgctaaagaa gccttggctc aagctaacaa acgcaaggaa
     9721 ccagaggaaa tctttgaaga agcggctaag ttagctgaac aagcacagca agttcaagca
     9781 gtggttgaag tcgtccctga gaaaaaagaa cctgcagtgg atacacgtcg taaaaaacaa
     9841 gctcgaccag acaaaaatcg tgacgattat gatcatgaag aagatggtcc tagaaaacaa
     9901 caaaagaatc gaagtagtca aaatcaagtg agaaatcaaa agaatagtaa ctggaataac
     9961 aacaaaaaga acaaaaaagg caataacaag aacaaccgta atcagactcc aaaacctgtt
    10021 acggagcgta aattccatga attgccaaca gaatttgaat atacagatgg tatgaccgtt
    10081 gcggaaatcg caaaacgtat caaacgtgaa ccagctgaaa ttgttaagaa acttttcatg
    10141 atgggtgtca tggccacaca aaaccaatcc ttggatgggg aaacaattga actcctcatg
    10201 gtggattacg gtatcgaagc caaacaaaag gttgaagtgg ataatgctga catcgaacgt
    10261 ttctttgtcg aagatggtta tctcaatgaa gatgaattgg ttgagcgtcc accagttgtt
    10321 actatcatgg gacacgttga ccacggtaaa acaacccttt tggatactct tcgtaactca
    10381 cgtgttgcga caggtgaagc aggtggtatt actcagcata tcggtgccta ccaaatcgtg
    10441 gaaaatggta agaagattac cttccttgat acaccaggac acgcggcctt tacatcaatg
    10501 cgtgcgcgtg gtgcttctgt taccgatatt acgatcttgg tcgtagcggc agatgacggg
    10561 gttatgcctc agactattga agccatcaac cactcaaaag cagctaacgt tccaatcatc
    10621 gtagccatta acaagattga taaaccaggt gctaacccag aacgcgttat cggtgaattg
    10681 gcagagcatg gtgtgatgtc aaccgcttgg ggtggagatt ctgaatttgt tgaaatctca
    10741 gctaaattca accaaaatat cgaagaattg ttggaaacag tccttcttgt agctgaaatc
    10801 caagaactca aggcagaccc aacagtgcgt gcaatcggta cggttatcga agcgcgcttg
    10861 gataaaggaa aaggtgcggt cgcaaccctt cttgtacaac aaggtacctt gaatgttcaa
    10921 gatccaatcg ttgtcggaaa taccttcggt cgtgtccgtg ctatgaccaa cgaccttggt
    10981 cgtcgtgtta aagttgctgg accatcaaca ccagtctcta tcacaggttt gaacgaagca
    11041 ccgatggcgg gtgaccactt tgccgtttac gaggatgaaa aatctgcgcg tgcagcaggt
    11101 gaagagcgtg ccaaacgtgc cctcatgaaa caacgtcaag ctacccaacg tgttagcctt
    11161 gaaaacctct ttgataccct taaagctggg gaactcaaat ctgttaatgt tatcatcaag
    11221 gctgatgtac aaggttctgt tgaagccctt tctgcctcac ttcaaaagat tgacgtggaa
    11281 ggtgtcaaag tgactatcgt ccactcagcg gtcggtgcta tcaacgaatc agacgtgacc
    11341 cttgccgaag cttcaaatgc ctttatcgtt ggtttcaacg tacgccctac accacaagct
    11401 cgtcaacaag cagaagctga cgatgtggaa atccgtcttc acagcattat ctacaaggtt
    11461 atcgaagaga tggaagaagc tatgaaaggg atgcttgatc cagaatttga agaaaaagtt
    11521 attggtgaag cggttatccg tgaaaccttc aaggtgtcta aagtgggaac tatcggtgga
    11581 tttatggtta tcaacggtaa ggttgcccgt gactctaaag tccgtgttat ccgtgatggt
    11641 gtcgttatct atgatggcga actcgcaagc ttgaaacact acaaagacga cgtgaaagaa
    11701 gtgacaaacg gtcgtgaagg tggattgatg atcgacggct acaatgatat taagatggat
    11761 gatgtgattg aggcgtatgt catggaagaa atcaagagat aagatttttt gctcctttct
    11821 taggtggtga gggacgcaag caaaccgatg gtttcattgc ttatttttga gcctagggtc
    11881 tcaaaaatcc cctgtgatgg gactgataaa tcagttccat cactttcacc acggcgaaag
    11941 aagcagatga cttcaaattg aacttcgttt caatttaaac tgaaaatcaa gaagtttaaa
    12001 atagctaggt ctgctggcct agcttttggt tcaaagtaga gaaaggaata tcatggcaaa
    12061 tcatttccgt acagatcgtg tgggcatgga aatcaagcgt gaagtcaatg agattttgca
    12121 aaagaaagtc cgtgatccac gtgtccaagg tgtgaccatc acagatgttc agatgctggg
    12181 tgacttgtct gttgccaagg tttattacac cattttgagt aaccttgctt cggataacca
    12241 aaaagcccaa atcgggcttg aaaaagcaac tggtaccatc aaacgtgaac ttggtcgcaa
    12301 tttgaaattg tacaaaatcc cagatttgac cttcgtcaaa gacgagtcca tcgagtatgg
    12361 aaacaagatt gacgagatgc tacgcaatct ggataagaac taaagaagag gggttgcccc
    12421 tcttttttgg tggaggaaaa taggttgaat ttgaaatgga aaaatattct tttaaaatat
    12481 attgaaacta gaatagtacg cctctacttc taaaacattg ttagaaatcg atttgactgt
    12541 cctgatcgat ttgtcctgtt cttgtttcat tttactataa aaaaggggtt ctgtattttt
    12601 taatgttatc taattagaaa atgctttttt tgtaggaaat ataatatgat aaggtgtaaa
    12661 aaagaaataa ggagtttgta tatggctgaa caagacttag ctatgcaagt attgcaacaa
    12721 gtggtgaaac tacctgttgt taaggttgat cgttcgaaat ttttagtgga taagttttcc
    12781 aaagaattgg atccaaaaga tattcctacc ttattggaac aaggtacaac gactcttcta
    12841 tctcaagaaa tattagatcg tgtagctaat gcttgtattc gggacaatgt attattagcg
    12901 agtgggactt ctgttttggc aggattagct ggagggcttg ctatggcaat taccattcca
    12961 gctgatgtgg ctcaatttta tgctttctct ctgaaattgg ctcaagaatt aggttatatt
    13021 tatggttatg aggatctttg ggcttcacgc gaggagttga gtgaagatgc tcaaaatacc
    13081 ctcttgcttt atctaggcgt aatgttaggg gtgaatggaa ccgctgcttt gctatgtgct
    13141 ggtggtataa caattgccaa acaggtaatg aaaacagtgc ctaataaagc tttaacaaag
    13201 acgctttggt accctatttt gaaaaaagtc ttaaaaatat ttggtgtgaa tcttaccaag
    13261 ggagggctgg ccaaaggaat agggaaattt attcctatct tgggtggtat cattgcaggt
    13321 ggtttaacct ttgcaactat gaaaccaatg ggggaaagct tgcagaaaga attatccaag
    13381 ctagtcaact atagtgaagt tcaatatcaa gaagatgttg aaacaatccg aaaagaggct
    13441 gaaatcatta aaggagagta atatgaatcc tatcaaagct tttgataaaa tttatggtaa
    13501 ttactttttg accgtgcaag gtgtaaaagt gatgaaaacg ataaagaaag ctgaccatgt
    13561 cgttgttggt ctgggaaaac tttttattgc agataagcta atggatacgg ctcggtggct
    13621 cattaggcca gaggagagag aatgaaattt tttttggtct tcttgctatt ctttttatca
    13681 aaccgattat tgggattgtg aaattctttt ggatgatcat ttcctttgca gttcaattgc
    13741 tgttttacaa gatattattt aagatattgg attggctctt taaacttatc tagatggtaa
    13801 tccaagtcgc agagagctag caggaactcc actgctagtt ttttaatctc tctccatatg
    13861 gtataatata agcagtaaaa tcattttata ctcttcgaaa atctcttcaa accacgtcag
    13921 cttcaccttg cagtatatat gttactgact tcgtcagttc tatccacaac ctcaaaacgg
    13981 tgttttgagc tgacttcgtc agttctatcc acaacctcaa aacggtgttt tgagctgact
    14041 tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc tgacttcgtc agttctatct
    14101 acaacctcaa aacactgttt tgagcaacct gcggctagct ttctagtttg ctctttgatt
    14161 ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc gatgtgtcaa
    14221 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagctgg
    14281 gttttaaacc ccttgccaat cccttgatgc gcaatacagt tggtcgtaaa gtatcactta
    14341 aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
    14401 atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg tcctacggga
    14461 tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc gttttgatgc
    14521 gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc tgatgaactc
    14581 agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
    14641 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
    14701 tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc gtagattgtt
    14761 agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta agggtttggt
    14821 gcgtcagatg ggacttgtgg gtcaatttga tatccattac caacgtaagg aagaactctt
    14881 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
    14941 tgatcagatt agggaactct ttcaaacagc tctaacgata gccaagtcac taccagaagt
    15001 gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg aaagtatgat
    15061 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
    15121 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
    15181 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
    15241 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
    15301 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
    15361 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcacctcc ctatggagat
    15421 tacctttgtc aataaagatg atattttcca gtattacaat gacaatacgc cagctgatga
    15481 gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct gccatccgcc
    15541 taagtacttg gacaaggtca aaactatcat gaaggggctt cgtgagggga ccaaggacaa
    15601 gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca cctatgctgc
    15661 agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg atatccagcc
    15721 ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa aaatgagtta
    15781 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
    15841 catggcgcac aaggaaagtc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
    15901 tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
    15961 aaacttcaaa gcaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
    16021 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
    16081 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
    16141 cgtaaaaaac aattacgaaa tggcctccgt cgctcaggtg ccttttcaag tactgtgact
    16201 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
    16261 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
    16321 aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc acttgatcgt
    16381 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
    16441 aaagaactcc ttgccttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
    16501 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac ttgctacaat
    16561 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
    16621 cacaaatgtg tcaaaactgg ttgctaatga ctgaaaaaat gaaatatttc tgtcttttta
    16681 ttttaaagac taaagatgcg ggcttggccc gaaattgggt ggtaccgcgg ataaacacat
    16741 tcgtccctgt catgtagatg gcagggcttt tcttttgtgt cttagtcaaa ggaggttgtt
    16801 ataaaacaat catttaaaac taataaacta tattatggtt ttcctgtttt tattttagga
    16861 tatcaagatt agaattttgg ctataacatc acgacctgta gttcctctta tagtctagga
    16921 gattgggttg tgattggagt cgttgcgaga gagaatgccg cagagcagat caaacagtat
    16981 caaaaattta ctgtgaatat ttctgatgaa acttctatgc ttgcgatgga gcaggctggt
    17041 tttatcagtc atcaggagaa attggaacgt ttgggagtgc attatgaaat ttctgaacga
    17101 actcagattc ctattttaga cgcctgtcca cttgttttag attgtcgggt agataggatt
    17161 gttgaggaag acggtatttg ccacatcttt gccaagattc ttgagcgact tgttgcccca
    17221 gaactcctgg atgaaaaggg acattttaaa aatcaactgt ttgccccaac ctatttcatg
    17281 ggagatggat atcagcgcgt ttatcgctat ctggataagc gtgtagatat gaagggcagt
    17341 ttcatcaaaa aagcgaggaa gaaggatggc aagaactgag ctgccagata aaatcgaaac
    17401 agaacgtctc gttttacgag tccgtactgt ggcggatgct gaggatatct ttgactatgc
    17461 tagtttgcca gaggtcgcct atccagcagg ttttcctcca gtcaagacct tggaagatga
    17521 gatttattat ctggagcaca ttcttccgga gcgtaatcaa aaggaaaatc tcccagcagg
    17581 ctacgggatt gtcgtcaaag taacggataa gatcgttggc tctgtcgatt tcaaccatcg
    17641 ccatgaagat gatgtgctgg aaatcggcta taccttacac ccagactatt gggggcgagg
    17701 ttatgtgcca gaagctgcgc gtgccttgat tgacttagcc tttaaagatt tgggtcttca
    17761 caagattgaa ctaacttgct ttggatataa ccttcaaagt aaacgagtcg cggaaaagct
    17821 tggctttacc ctcgaagctc gcataagaga ccgaaaggat gttcaaggaa accgctgtga
    17881 cagtctgata tatggcttgc tgaagagcga gtgggaggaa taagatgagc gatgtaaaag
    17941 aggaagtaag ctctctatct gagaagcagt tacgccaaat tgatgttgag tatgctgaat
    18001 taaatgatag tgatattatc gagcgattag cttatttaga aattaacaac aatgaaaaac
    18061 gtattgtcat ctctgatatt gaaccaacca aggaaataat gtcagtctcg gaccaaattt
    18121 ttgagattca aaagaatttt caaaaaatca agaatatgtt cgaattgttt atttcagatg
    18181 tctcggattt tttaagtatt aaaaataaac ttgagtcaaa ggaattagaa attgaagagg
    18241 cagatgtaaa tcgtttcatg attcatttat tatccagtgg aaaattgttt gtcgatttta
    18301 acgaaaacca aattaaacaa aaatattcca aagatagtga ggagtttgat tgtatacatg
    18361 gttttgcaag ctatcagtat gatatcaatt ttacttatcg attttgccat tctttgagaa
    18421 attattcgga acatactgac ttaccaataa atgaggtaaa agctgttagc ccagatgatg
    18481 agactgttat aatagatttc tatattgatt tagattatct tttaaattcc aactttaaat
    18541 ggaaaaaatt gaaaggggag ttgataaaac taaaccaaga aacatctaag attgacgcta
    18601 tagctctagt gaaagaatac tttaatgctc ttacagagct ctatggtaat tataataagt
    18661 tgtttttaaa gttaaaccat aatacattag tagatattaa atcaaagttg gaatcattaa
    18721 aattgaaaca ctctaggtat tatatttcaa agataagcaa gtatgatctt aaatataatc
    18781 ctggaaatta tactatgtca ccacttgcag catttgctga aattgaagag atttatattg
    18841 aactttcaaa aattggtttg gttaaaattg tcaataaaag taattaagga gaacacacat
    18901 gtctaaagaa ctttcatcta aatacaatcc agccgaggtt gaggctggtc gttaccaaaa
    18961 atggcttgat gcggatgttt tcaagccttc aggcgatcaa aaggctaagc cttattcaat
    19021 cgtgattcca ccaccaaacg taactgggaa acttcacctt ggtcacgctt gggatacgac
    19081 tttgcaggat atcatcatcc gtcaaaaacg catgcaaggc tttgataccc tttggcttcc
    19141 aggtatggac cacgctggta ttgcgactca ggctaaggtt gaggagcgct tacgtggtga
    19201 gggtattacg cgttatgacc taggtcgtga gtctttcttg acgaaggtct gggaatggaa
    19261 agacgaatat gccactacca tcaaggaaca atggggcaag atggggcttt ctgtagacta
    19321 ttctcgtgag cgtttcactc ttgatgaagg tttgtcaaaa gctgttcgta aggtctttgt
    19381 gaacctttac aagaaaggct ggatctatcg tggtgagttt atcatcaact gggacccagc
    19441 agctcgcaca gccctttctg atattgaggt gattcacaag gatgtagaag gtgccttcta
    19501 ccacatgaat tacatgctgg aagatggttc acgcgccctt gaagttgcta caactcgtcc
    19561 tgagactatg tttggggacg ttgcggttgc gattaatcca gaagacccgc gctacaagga
    19621 cttgattggt aaaaacgtca tccttccaat cgctaataaa ctcatcccaa tcgttggaga
    19681 tgagcacgca gatcctgagc ttggtactgg tgtcgtgaaa atcacacctg cccacgatcc
    19741 aaatgacttc ttggttggcc aacgtcataa cttgccacaa gtcaacgtca tgaacgacga
    19801 cggaactatg aatgagcttg cctttgaatt ttcaggcatg gatcgttttg aagctcgtaa
    19861 ggcagtcgtt gctaagttgg aagaaatcgg tgcccttgtc aaaatcgaaa aacgtgtcca
    19921 cagtgttggt cactcagaac gtacaggtgt cgtagttgag ccacgcttgt ctactcaatg
    19981 gttcgtcaag atggaccaat tggctaagaa cgccattgct aaccaagaca cagaggacaa
    20041 ggtcgaattc tacccacctc gtttcaacga taccttcctt caatggatgg aaaatgtcca
    20101 cgactgggtt atctctcgtc agctttggtg gggtcaccaa atccctgcct ggtacaatgc
    20161 tgatggtgaa atgtatgtcg gcgaagaagc tccagaaggt gacggatgga ctcaggacga
    20221 agacgtcttg gatacttggt tcagttctgc cctctggcca ttttcaacca tgggctggcc
    20281 tgaagtcgac tcagaagact ttaaacgtta tttcccaact tcaaccttgg taacaggtta
    20341 cgacatcatc ttcttctggg tatctcgtat gatcttccag tccttggaat ttactggtcg
    20401 tcagccattc caaaacgtgc ttatccacgg tctcatccgt gacgagcaag gacgcaagat
    20461 gtctaaatct ctcggtaacg ggattgaccc aatggatgtc atcgagaaat acggtgctga
    20521 tgcccttcgt tggttccttt caaacggttc cgcaccaggt caagacgttc gtttctctta
    20581 cgagaaaatg gatgcttcat ggaactttat taacaaaatt tggaacattt ctcgctatat
    20641 cctcatgaac aatgaaggtt tgacgctgga tgtggcgcat gacaatgtca caaaagttgc
    20701 aacaggtgag gctggtaatg tgacggaccg ctggattctc cacaatctca acgaaaccat
    20761 tgcaaaagtt actgaaaact ttgataagtt cgagtttggt gtggctggtc atatccttta
    20821 caacttcatc tgggaagaat ttgccaactg gtatgttgag ttgaccaagg aagtccttta
    20881 tagcgataac gaagaagaga aagtcatcac acgttctgtt ctactataca ctttggacaa
    20941 gatccttcgt ctccttcacc caatcatgcc atttgtgaca gaggaaatct ttggacaaat
    21001 ctcagaagga tctatcgtta cagcagaata cccaactatc aacccagcct ttgaagacct
    21061 tgctgctcac actggtgtgg aaagccttaa agacttgatc cgtgccgttc gtaatgcgcg
    21121 tgcggaagta aacgttgctc caagcaagcc tatcactatc cttgttaaga caagcgatag
    21181 cgacttggaa gccttcttta acagcaatgt caactacatc aaacgtttca caaatcctga
    21241 acacttggaa atcgcatcaa ccatccctgc acctgaactg gctatgtcaa gcgtcatcac
    21301 aggagccgaa atcttcttgc cactggtaga cctcttgaat gtcgaagaag aattggcacg
    21361 tctggaaaaa gaacttgcca aatggcagaa agaactcgac atggttggca aaaaactcag
    21421 caacgaacgc ttcgtcgcca acgctaaacc agaagttgtc caaaaagaac gcgacaagca
    21481 agcagattac caagctaagt atgatgcgac cgtagcacgt attgatgaga tgaagaagtt
    21541 ggtgaaataa acacagaaac acggtgatga gccgtgtttt ttggtataat gaaatcaata
    21601 tctattgatg gagaaagtga tgtccaaaca ccctcattat gaattgttaa atttaattgg
    21661 ctacggtctt gccaagtttg acaagctttt tataaaagaa tttcaatgct ctagcaagtc
    21721 ggagttttat cgctatgtgg tttctctggg aattgctgaa acaactggag ttgtaaaaaa
    21781 tagaatggat ttatttgatc cttattttga caataatcga aaaggttggt ggcagaaagc
    21841 tgaagtttat cgttttcgta aagatttaat tgatatgatg tttggaaatg aagatgttca
    21901 tagttatgct gaaatagtta aaatgttact tgccagtgaa gggaagaaaa caggcataac
    21961 catcgttgaa aaaccaatag ttcgaactaa attcaaacgt ctacaggaaa cgggaatgga
    22021 ggctgagaat tattttatcc ttcattttga taaagaagaa aaattccaag gtggacagtt
    22081 aaccgatgcc cgtctttatg gtgatggata tgactttcag gtagatgtcc aagagtattc
    22141 ttaccttgcc gaagtaaaag gtatccgaaa atctaagggg cgtgtgcgtt taactgcaaa
    22201 agaatttgaa aaagtcaagg agtttcaatc ggattttatt ttatccttgg tcacaaatct
    22261 agatgatatt ccaaagttag tgttaatcga taatccttta aaacattttg agtttaaaaa
    22321 gaatattatc aaaaatgaaa tcattgaata tagaagcgta gaggatttgt attagttccc
    22381 ccttaacttt tccagttgaa ggaacgagaa gtcttgacag gtcaacgact taatgaactg
    22441 gaaatcaacg gtattcgttt gactaagttt taagatggcg aaatcggtat tgaattta
//
LOCUS       DAYYQN010000034        21599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYYQN010000034 DAYYQN010000000
VERSION     DAYYQN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.034"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(145..1341)
                     /gene="tuf"
                     /locus_tag="ACHBRQ_001265"
     CDS             complement(145..1341)
                     /gene="tuf"
                     /locus_tag="ACHBRQ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9592934.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2252..3121)
                     /gene="gla"
                     /locus_tag="ACHBRQ_001266"
     CDS             complement(2252..3121)
                     /gene="gla"
                     /locus_tag="ACHBRQ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HFM9592935.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3345..4022)
                     /locus_tag="ACHBRQ_001267"
     CDS             complement(3345..4022)
                     /locus_tag="ACHBRQ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9592936.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLASLVLGFF
                     KKKEDEK"
     gene            complement(3980..5059)
                     /locus_tag="ACHBRQ_001268"
     CDS             complement(3980..5059)
                     /locus_tag="ACHBRQ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9592937.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDIVEPSRTVVEKIIPKLNIPEEEKKKKTSELAE
                     LISENVKVCYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNVTDKKLSSMT
                     TTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKEGSQEKL
                     AESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAYQKTVQQ
                     LTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5170..5424)
                     /locus_tag="ACHBRQ_001269"
     CDS             complement(5170..5424)
                     /locus_tag="ACHBRQ_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592938.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPIWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5418..5660)
                     /locus_tag="ACHBRQ_001270"
     CDS             complement(5418..5660)
                     /locus_tag="ACHBRQ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592939.1"
                     /translation="MVQNEDLYKKGLNVELAHQQIKGFFEAEFKNRINGVLNTKIKNS
                     TLNRVNKKTIHQSNKNSMINLKQKQRKMLKNKAILC"
     gene            complement(5764..6609)
                     /locus_tag="ACHBRQ_001271"
                     /pseudo
     CDS             complement(5764..6609)
                     /locus_tag="ACHBRQ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            6843..8561
                     /locus_tag="ACHBRQ_001272"
     CDS             6843..8561
                     /locus_tag="ACHBRQ_001272"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9592940.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNETAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8672..9019
                     /locus_tag="ACHBRQ_001273"
     CDS             8672..9019
                     /locus_tag="ACHBRQ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9592941.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9297..10133)
                     /locus_tag="ACHBRQ_001274"
     CDS             complement(9297..10133)
                     /locus_tag="ACHBRQ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9592942.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10146..10775)
                     /locus_tag="ACHBRQ_001275"
     CDS             complement(10146..10775)
                     /locus_tag="ACHBRQ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9592943.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10785..11426)
                     /locus_tag="ACHBRQ_001276"
     CDS             complement(10785..11426)
                     /locus_tag="ACHBRQ_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9592944.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11572..12801)
                     /locus_tag="ACHBRQ_001277"
     CDS             complement(11572..12801)
                     /locus_tag="ACHBRQ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9592945.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12791..13957)
                     /locus_tag="ACHBRQ_001278"
     CDS             complement(12791..13957)
                     /locus_tag="ACHBRQ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9592946.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14101..15114
                     /locus_tag="ACHBRQ_001279"
     CDS             14101..15114
                     /locus_tag="ACHBRQ_001279"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9592947.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15159..15578)
                     /locus_tag="ACHBRQ_001280"
     CDS             complement(15159..15578)
                     /locus_tag="ACHBRQ_001280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9592948.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15589..16995)
                     /gene="atpD"
                     /locus_tag="ACHBRQ_001281"
     CDS             complement(15589..16995)
                     /gene="atpD"
                     /locus_tag="ACHBRQ_001281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9592949.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17081..17959)
                     /locus_tag="ACHBRQ_001282"
     CDS             complement(17081..17959)
                     /locus_tag="ACHBRQ_001282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9592950.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(17975..19480)
                     /gene="atpA"
                     /locus_tag="ACHBRQ_001283"
     CDS             complement(17975..19480)
                     /gene="atpA"
                     /locus_tag="ACHBRQ_001283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9592951.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19495..20031)
                     /locus_tag="ACHBRQ_001284"
     CDS             complement(19495..20031)
                     /locus_tag="ACHBRQ_001284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9592952.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20031..20525)
                     /gene="atpF"
                     /locus_tag="ACHBRQ_001285"
     CDS             complement(20031..20525)
                     /gene="atpF"
                     /locus_tag="ACHBRQ_001285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9592953.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20539..21255)
                     /gene="atpB"
                     /locus_tag="ACHBRQ_001286"
     CDS             complement(20539..21255)
                     /gene="atpB"
                     /locus_tag="ACHBRQ_001286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9592954.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLPYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21290..21490)
                     /locus_tag="ACHBRQ_001287"
     CDS             complement(21290..21490)
                     /locus_tag="ACHBRQ_001287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFM9592955.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         6433 a         4653 c         3952 g         6561 t
ORIGIN      
        1 atgtactatt ctagtttcaa tatattatag tttgctcttt gattttcatt gagtattagt
       61 tcaatatcta aaaaaagaac cttgccaagc aagattcttt tagtgtctga cttaaataat
      121 tgttcttctg ggaactaaat cgaattaagc ttcgatttct gtaaccatac ctgaaccaac
      181 agtacgtcca ccctcacgga tagagaatgt agtaccttgt tctacggcga ttgggtgaat
      241 caactcaacg tcgattgtca cgttatcacc aggcattacc atttcagtac ctgctggaag
      301 ttcgattgaa cctgtaacgt cagtagtacg gaagtagaat tgtggacggt agttgttgaa
      361 gaatggagtg tgacgtccac cttcttcttt agtaaggatg tagacttcac ctttgaattt
      421 agtgtgtggg ttgattgaac ctggtttagc gataacttgt ccacgttcga tttcatcacg
      481 ttgaacacca cgaagaagga cacctacgtt atctccagca agaccttcgt caagttgttt
      541 acggaacatt tcaacaccag taacaactgc tttttgagtt tcttctttga taccaacgat
      601 ttcgatttcg tcgttgactt taacgatacc acggtcgata cgtcctgaag caactgtacc
      661 acgtccagtg attgagaata cgtcctcgac tggaagaagc aatggtttgt cagtgtcacg
      721 ttctggttct gggatatact catcaactgt gttcatcaat tccataacga tgtcttcgta
      781 tttagagtca ccttcaagag ctttaagtgc tgaaccttgg ataactggaa gatcgtcacc
      841 tgggaagtcg tattctgaca ataggtcacg gatttccatt tcaaccaatt caagcaattc
      901 ttcgtcgtca accaagtcaa ctttgttcat gaagacgata aggtgtttaa caccaacctg
      961 acgtgaaaga aggatgtgct cacgagtttg tggcattggt ccgtcagttg aagctactac
     1021 aaggatagct ccgtccattt gagcagcacc agtgatcatg tttttaacgt agtccgcgtg
     1081 tcctggagcg tcgatgtgag cgtagtgacg tttttcagtt tcgtactcaa cgtgcgcagt
     1141 gttgatagtg ataccgcgtt cgcgttcttc tggagcagca tcgatagacg catagtcttt
     1201 aggttggtta actgatgaag gcaagcgacg tgccaaaaca gttgtgatag ctgcagttag
     1261 ggtagtttta ccgtggtcaa cgtgtccgat agtaccaatg ttaacgtgtg gtttactacg
     1321 atcgtatttt tcttttgcca tttgagtaaa agcctccaat aaaatatatt ttatagatag
     1381 acagtaggca atacagtcta actttcctta ctattttatc aaatttcagc taaattgcaa
     1441 gtgttttaca acgtttttgt gaattattct tggtaaaaat ttaactaact aatagaaagt
     1501 actaaataga cttatccttc aaggagtttt cagcattatc catccactca cttgctgaaa
     1561 atgtaaacat tctctggttt atatagtaaa atgaaacaag aatagggcaa atcgatcagg
     1621 acaatcaaat cgatttctaa caatgtttta gaagttgagg tgtactattc tagtttcaat
     1681 ctactataca acctcaaaac tgtgttttga acaacctgcg gctagcttct tagtttgccc
     1741 tttgattttc attgagtata aaattgcaga taaaacgata acaatcatag gactcagcag
     1801 agtcaatgta ggtcgaagaa tagcccccct aatcgcttat tttatagcaa aatgaaatag
     1861 aaactgaaca ttttgttgag gaaggtaaat caattcctca caatgtttga gaaagaaggg
     1921 aggactattc caacttcaac ccactataaa gcagacttac aagataggga acccatctcc
     1981 gataatatat agtagaatga aaactgcaca atttgattgg agaattcaaa ccaatttcta
     2041 acaatgtttt agaaacaagg gtctattatt tcaactgcaa cctactatag cccattgaag
     2101 ctggaatagc ccactgagac ttctaaacca ctgctggaga ggagtttgtt tagagtttct
     2161 ttcatttccc taaaaaatca aaaagagaga accagtctga tcctctctta cttatagatg
     2221 gtgctcactc aaatgttaaa ggagctattt cttagagata aaggaatttg aatacagcta
     2281 ctgccgcaat tgctgctgcg ataggtgcta ctactggtac ccaagaatac caccattttg
     2341 aatcgccttt atgctcacca agaactgatt ttggaaggaa agcatgaagg agacgtggtc
     2401 ccaagtcacg ggctgggttc aaggcaggtc ctgtaggtcc tccaagtgat gttaccaaag
     2461 ccataacgag gaatccaagt gccaagtgag ccacagaaag tcctgaagca gtgtgtggag
     2521 ccacctgtgc tttaatagcc aagtcagaaa aatcaactgt ttgtcctgct tctattgcct
     2581 tttgtttcat gaattgaagc acttcagcac caaagaagtt tttagtcaaa ccaagagctg
     2641 caaagaacaa aacaaatgaa ccaacaaact cattaatcaa accattgaca gttgctgcat
     2701 agcgactttc ttttgtacca tggtcaatac ttgaaatagt tgagaaagtt cccaagatgt
     2761 tatttggttt ttcagttttc aagtagaatg gacggtatgt tgccacaact aaggcttggc
     2821 caaagatagc ccccaagact tgcgcgataa tgtaaggtac cacttgtgcc caagggaaaa
     2881 gaccgctaac tgcaagccct agagtgaaag cagggttgat gtgattccca gatacgttac
     2941 caaacatcaa ggctgggatc ataaccccca taccataacc aacagcgatg acgatccagc
     3001 cactttggtg acctttcgta cctttaagtt caacgttggc aactgcacca ttcccaagaa
     3061 tgatcaaaat ggcagttccc aaaaattcag tggcatactt cagtgcccat gtgaaatcca
     3121 tttgctagat tctccttaaa attttttaga tattccttat tttatcaatt tttaccctat
     3181 ttagcaactg atttctggaa atatttttaa aaaaatagga ttcatagttt cccagttcaa
     3241 cgtacttgta agcgttttta ttcttcacga aagacctcgt tctcaaaacc aggaaaaaag
     3301 cccacatctg tgagcttttc ctggttccta gattctaaaa tctattattt ttcatcttct
     3361 tttttcttga agaatcctaa tactagacta gctaacaagg tcatgatacc agcactagcc
     3421 aaggtagcat tagcttctgt acctgtgttt ggcaattcct ctctcttacc tgtctcataa
     3481 gtcggaactt ctgggtctgg attcactgga gtttcagttt ttggagtacc tggttctgga
     3541 gttggctctg gtattggagt ctcttgattt ggtagttctg ggtttggtgt cggaacactt
     3601 ggatgctctg gatttggttg tgggattggt ttttctggag tcggttcacc tggagtacct
     3661 ggttcctgag gacttggaat ttctggtttt tctggagttg gtttatctgg tgttgataaa
     3721 cggtcatacc ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca
     3781 actgttggaa tatctggatc tttgtacttg tcaatcttac cagatataac ctcgtcccag
     3841 tttcctgttg tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta
     3901 cggcttaagg ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca
     3961 acgtaatgaa tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc
     4021 actgtttcct caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg
     4081 catttgcagg agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt
     4141 tgtcctcagt tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta
     4201 tcgctaaatc agcttttata acagactctg ccaacttctc ttggctacct tctttatagt
     4261 aattggctgt tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg
     4321 ttctaccttc gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa
     4381 aagtataata ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattat
     4441 agctggtacc aatcagttgt tttttattgg taatgtaagt tccgtcactt tctttttctc
     4501 caattccagt atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcaaactt
     4561 tcacattttc tgagattagt tctgccaatt ctgaggtttt tttctttttc tcttcttcgg
     4621 gaatatttag ttttggaatg attttttcaa caacggttcg tgatggttcc acaatatctt
     4681 ggatgactga aaagtcagct agaattggga gattatactg aacacggtga ctttgagtgt
     4741 ttactcctac tctttcatta ttctctgaaa atacttgtac ggtataagaa acaacatctt
     4801 ttcctaatag aacatcccca gtagagaaat agccgccttt tcctagtttg ctatctccag
     4861 agtccacttc tttcctaatc ttatcagata gttttttacc agtcagtaca ttcgttcgca
     4921 caatcccttt gtctacccct acaaagtggg agaacttttt gaactcttca gaaccagatc
     4981 tagcccaacc attattaagg gcatttgctt ttgtatttgt attctctctc aaaggtttgg
     5041 cgattagaat tatattcatc ggcacttaga gttgctgcta tatctgactc ttgaatacca
     5101 acttccttac taccatttct agcggcagta tatgtgtatt tatctgtttc tacttctagc
     5161 tgattatcct taacatgata ggtaattttc tcctggtata agagtcttca gtagtttata
     5221 tgctctgtca tcatctttag ctttagaagc agctgagttt agccaaattg gggtattagg
     5281 agtatcttta tcagcctgtt tcaagacatt attcaagtca tttgctaacc tattggttgt
     5341 cctcactgtg aaagttttct tccagtctga accactttta ttagacttag atatgctcat
     5401 gaaatcctgg tcaacattca acacaatatc gccttgtttt ttagcatctt ccgttgcttc
     5461 tgcttcaaat tgatcatgga gtttttgttg ctctggtgta tagttttttt atttacacga
     5521 tttaatgtac tattttttat tttagtatta agaactccat taatacgatt tttaaattct
     5581 gcttcaaaaa atcccttaat ttgttggtgc gcaagttcaa cattcaaacc tttcttatac
     5641 aagtcttcat tctgaacgat ttcggctaca cggtgaatgg tttgatcgta taccgtcttc
     5701 tcttaaccag cagtatcctt agttttaatg actactcgat aggttccttt agaaccgtta
     5761 tagtcatcta gttttaaaaa agcattccaa acattcaaaa atagttggaa cttcccttaa
     5821 taaatccgtc actttctttt tcaagatagc ccaagcttgc tcaataggat tcaaatccgg
     5881 tgaataaggt ggtagaggta agaaaaagtg tttatcttgg atgcagagtt catccaaaat
     5941 gttcttggga tgaaaactag cattgcccat gacaataaca tgaggtgtct tcaaagcagg
     6001 taggagttgc gttttgaacc actccacaaa gaaatcgctt gtcatgcttt tcttgtaaat
     6061 catgggagct ataaactctc cgtctacttg tcctgcaaca attgaagttc gctcaaaacg
     6121 acgtccgcta atcttttcat agactttctc ccctctagga gcccctgcat aaggacgata
     6181 gaggtagcgg tcgattcctg tttcgtcaat atatacgatt ggtaaatttt ttaggttatc
     6241 caaaatatca agaaactcag cgactttctc tggctcttgt tccttaaagc tcgtcatctt
     6301 tttttaaagt gaccttaatc tgctttaaag ctgcccatac tgaaggaaca gcacaatcaa
     6361 aatgtgccgc aatttcccgt aaaaaagcat ctggatgagc ctctacaaag gctttcaatt
     6421 cctctaaagg aatctttcgg tttttgacga ctcgcttttt cctctctatg tgtccttgtt
     6481 cacatctttc ttttcccacg tgaagagagt tctgacgcca acaccaaaaa acttggctgc
     6541 ctcgacatgg ctgtgcccct ctttgatgta atctaatgct cgctgtttaa aatcagtact
     6601 atatgccata gctttattat aatatgcttg tttaaaacta agcaactata tcaatgtttt
     6661 gttattagaa gacttacttt ttttactgaa atcgaatttt tcctaacctc aacatcatat
     6721 aaccccaaaa ttcaatctga cctggtttga agagatgttc gaagcagata aggttttact
     6781 aaaactaaaa aaaatgatat aatcaaactg attaaatgtt ttaaaaataa aggagaattt
     6841 gaatgtctta ccaagaaaat taccagaaat gggttgattt tgtggagctt cctgactacc
     6901 ttcgtcaaga tttggaaaat atggacgaaa aaactaagga agatgccttc tatacaaatc
     6961 ttgaatttgg tactgcaggt atgcgtggct tggttggtgc tggtacaaac cgtatcaaca
     7021 tctacgttgt tcgccaagct actgaaggat tggctcgttt gattgagtca aaaggtggaa
     7081 acgagaaaga acgcggtgta gcaattgcct acgatagccg tcacttctca cctgagtttg
     7141 cctttgaatc tgcggcagtt cttgctaaac acggcatcaa atcttacgta tttgaaagcc
     7201 ttcgtccaac tccagaacta tcatttgcag ttcgtcatct caactgtttc gcaggtatca
     7261 tggtcacagc cagccacaac cctgctccat ttaacggtta caaggtttac ggtgaagacg
     7321 gtggacaaat gcctccacac gatgcggacg ctttgactac ttatatccgt gcaatcgaaa
     7381 acccatttgc agttgaagtt gctgatgtgg aaactgaaaa agcttctggc ttgattgaag
     7441 ttatcggcga agctgttgac atagaatacc ttaaagaggt taaggacata aacatcaacc
     7501 cagccttgat tgaagaattt ggtaaagaca tgaagattgt ctacacacca cttcatggta
     7561 ctggtgaaat gttggctcgt cgtgctcttg cccaagcagg atttgactct gttcaagttg
     7621 ttgaagcgca agcaactgct gacccagact tctcaactgt aacatctcca aacccagaaa
     7681 gccaagcagc ctttgccctt gctgaagaac ttggtcgtca agttggtgca gatgttcttg
     7741 tcgcaactga cccagacgct gaccgtgttg gtgttgaagt tcttcaaaaa gatggtagct
     7801 acctcaacct ttcaggtaac caaatcggtg ctatcatggc taaatacatc ttggaagctc
     7861 acaaaaacgc tggaactctt cctgaaaatg ccgctctctg caaatctatc gtttcaactg
     7921 acttggtaac gaagattgct gaaagctacg gcgcaactat gttcaacgtc ttgacaggtt
     7981 tcaaatttat cgctgaaaaa atccaagaat tcgaagaaaa acacaaccac acttacatga
     8041 tgggatttga agaaagcttc ggttacttga ttaaaccatt tgtacgtgat aaagatgcca
     8101 tccaagccgt tcttgtcgtt gctgaacttg ctgcctacta ccgttctcgt ggtttgacac
     8161 ttgctgacgg tatcgaagaa atctataaag aatatggcta ctacgcagaa aaaacaatct
     8221 ctgttactct ttcaggtgtc gatggtgctg aacaaatcaa agcgattatg gctaaattcc
     8281 gcaacaatgc tccaaaagaa tggaacgaaa cagctatcac tgtcgtagaa gacttcaagg
     8341 cacaaactgc tactgttgct gacggtactg ttacaaactt gacaactcct ccaagtgatg
     8401 tgttgaaata cacacttgct gacggttcat ggattgccgt tcgcccttca ggtacagaac
     8461 caaaaatcaa gttctacatt gcagttgtag gggaaaccaa cgaagaatca caagctaaga
     8521 ttgctaacat cgaagcagaa atcaatgcat ttgtaaaata aagacctata aaagatgaga
     8581 ccaaccaacc tcatcttttt tcgtatcaaa tctaagcaat tttagaaact ttatggcata
     8641 ctagacttat caatgaaagc gaggtaatct catggaacaa tttttagata acatcaaaga
     8701 ccttgaagtc actacagttg tgcgtgcgca agaagctctt gataaaaaag aaactgcaac
     8761 cttctttatc ggtcgcaaaa cttgccctta ctgccgtaaa tttgcagata cattgtcagg
     8821 tgtcgtagct gaaaccaaag ctcacattta cttcatcaat agtgaagaac caagccaact
     8881 caatgatttg caagcattcc gctcacgcta tggaatccca actgtaccag gttttgttca
     8941 tattacagat ggacaaatca acgtccgttg cgactcttca atgtcagcac aagaaatcaa
     9001 agatttcgca ggattgtaaa aaatcagcta acaagtctta aaatcacaaa aacgcataat
     9061 gtaaggtgca aaaaccttga tattatgcgt tttattgttc ctaaaacagt tacatcagca
     9121 tttcattaca gaacatttta attttggaag tcaaatcaat ttttaacaat gttttagaaa
     9181 tcacagtggg ctattccata ttcaattatc catattcaat cgctattatc gttttcctag
     9241 attacaaaaa aactacgttt tgctaatcaa aacgcagttt cagccactat tttatcttac
     9301 tgtccttctt ttacttcttt ggttgctaca tcttctccaa accatttttg gctgatttct
     9361 tggaacttgc cgtccttgta aagactagaa aaagcttcat ttatcttctt aaccaaggtt
     9421 gtatcttcct tacgggctcc aaccgcaaaa gcttctgttt ctagtccaac tgtaaagaca
     9481 ttataatcgt ttaaaacacc ttctgcttct aaataatagt ttgcatagac acggtcaatc
     9541 aatagaccat caattcgatc gtttttcaaa tcaatcaagg cttcattaaa ggtttggtat
     9601 tgattcgctt ccttattagc gacaatattc ttcaaaattt ctggatttgc ttcaaagtcc
     9661 gcataaccag atgaaccagc ttgagctcct aatgtctttc cagtcatatc ctttgcagtc
     9721 gtgataccag atgatttctt cgtaaccaat acctgctcat tcttcatata tgagttactg
     9781 aaagccacct tttcacggcg ttcgtctgta gcggaatagc cattccaaat cagatcaatc
     9841 gttccttttg tcaattcagc ttctttcaaa tcccaatcaa tcggttgcca atttaccgtg
     9901 attccgtatt tttcaaaaac agctgtagct aaatcaatat caaatcctgc ataagaacca
     9961 tctttctgag caaatcccat tggaacaaaa gtactatcaa atccaatagt aatagacttg
    10021 ttagactggt actttgacca attatctcca ctagtttcgc tagaattttt cccacaagct
    10081 actaagaaca aagcagtcat cagactgact aatacaagca tccatttttt catccatttt
    10141 tcctcctatt taggttctac tttcaataat acatctgcga tattttcagc aaactgcaaa
    10201 tcatgggtaa ccacaatctg ggtcatccca agttccctat tttgcaagat tagcttctcc
    10261 acttccaaac gtaattctgg atccagggca gaagttggtt catcgtagcc aatgatttct
    10321 gggtcaatca tcatagcacg cgccaaagcc acccgctgct tttgcccacc agatagtgag
    10381 aaaggatagg cctctgcgtg tcctcctagt cctaactgtt ccaagagtcc actcgccttc
    10441 ttctcagcct cttcctgctt cattcccatg gtcttcacag gcgataaagt caaattttcc
    10501 agaactgata gatgaggaaa tagttgaaaa tcttggaaga caaatcccag taggttgcgc
    10561 ttctgcaatt catctagctc taaaggttgt ccattataaa agatttgccc tgaatcaatg
    10621 gtttcaagac ctgcaagcat acgtaaaaga gttgtcttac ctccaccaga aggtccaacg
    10681 atagccagga tttgcttttc aggaatactt agactgaaat cagacaggat ttgtttgtct
    10741 ccaaagactt tattgatatt tcgtaattct aacatagctg ccccctatct ataataactg
    10801 tactttttct caactttttt ggaaataatt gtcacaatcc caattaaaat caagtaaatg
    10861 gctcctgcca agaacatagg aaccagacta gcatcgcggt tagcagctgt tcgactagcc
    10921 aagataaggt ctgaaattcc gagagcatag accaaagaag tatccttgac caaactcata
    10981 acttcattaa agacactagg aagaacgatc ttggtcactt ggggcaagat aatatagcgc
    11041 actctgtcaa aagggctaaa cttcaagacc ttggcagcct catactgtcc tcttggaata
    11101 gtgtcaattc ccccacggaa aatttctgca aagtaagctg catagttgag aacaaaggca
    11161 ataatagctg caggaaggcg gtctaaacga atcccaatac ttgggagcac ataatagata
    11221 aaaatcagtt gcaagagtaa gggggttcct cgcataaccc agatgtaaat gttaatcaga
    11281 taatggaggg gcttccaatg gacttgcaag gcaaaggcaa tcaaaacgcc caagggaatc
    11341 gaaaatagca agaccagtgc aaagacctgt acagtcgtgc tcgcaccatt cagtaaactc
    11401 ggtaatatct caaataaata agacatactg cacctcctaa aagtaattgt tcctattata
    11461 gcatgaataa acaagattac caagtatttt tgtaaaaaga ttctaccata tacaaataaa
    11521 aaccgtagct atcaaaagag ataaactaca gttctatatg tttaaacatt cttacaatct
    11581 ctcaaacagt tcttgcattt gcacatcatc tggaaccagt tttaagtaag tgtgagcatg
    11641 gacttttgct tcttcaaaat gtcccaattc acgcaagaga tagatatagt gttccagaaa
    11701 ttctggattg tccttcaaat ctcctgtcaa ctcttgataa tgctcataag cagtatccaa
    11761 atcgtccatt tcttgataag aacgagcaat catccacttg gtcaaaagat tttctggctc
    11821 ctcactctgc aagtctagaa tatcctcata acgctcctgc tccagataaa tagtggctaa
    11881 acgaagcaag atttcttctg tatcctcagc gtcttctttt gcagtaagga gataattttc
    11941 tgcaccacta gcatcatgca attcataaga aaattgtgaa gcagctagca agaggcgagt
    12001 ttcaaaggga tttttctcta atccttgctt agcgatacgc agggcttctt gaacttgatg
    12061 ttccttatgt aaagcctgac tgtacccata ctcatagcct tcaaagtcag gagaaatggt
    12121 atcaagctgc ttaaagtaga gggtggcttt ttgatattct tcttgatcaa aataaagact
    12181 ggccaactca aaagctgtta agtcatcgta ttctaactcc agggcttttt ctaaaaactc
    12241 agtagccgtt tcaaatttcc ctaactgagc ataggcaaag ccaattcgtt gataggtgga
    12301 aatgcccgtt tgctcataaa tcgagcgatt atctaactgg gcataggctt gaatagccgc
    12361 ttggtaattt tccaactcac tatccaactc tgccaaaccc aatatcaaga gagaatcctc
    12421 tgagtaggtc aaggcctcca ataatttctc acgtgccaca tctgtcaaac cttccagctg
    12481 gtaaaggtct gccttcagag ccaaagacga gacataccag tcactgtcag cttggatttc
    12541 ctcaagatag gtaaaggctt cttctatttg accatcctcg ctagcaattg cagctagatt
    12601 aagatgaacc tctggaaaat cctctacaat tttcaggtaa atttccttgg cctgaggata
    12661 gaaaccaatc ccttcaagat aagttgccaa ctcatacaga agatcacttg aatcattttc
    12721 taaagctttg gcgaaataat gctcagcctt agttaaatct tgctcctcca aagcctgtaa
    12781 catctgttga ctattgttca ctcttgactt cctcaccctc taattcatat agaccactga
    12841 ctaccttata ccattcgaaa atggctgaaa tgacaacctt agcagaggca taaaccggaa
    12901 taccaagtaa aactccccag ataccaaaca tagatcctga agttaacaaa acaaagagaa
    12961 cattaatagg gtggatgttt aattgacttc ccaaaatcaa tggagagaca aaacggcctt
    13021 caatagtttg ttctacgata aagacaatca ctactttcaa aagcatgact ggaccagcaa
    13081 tcaaacccaa tactagagca ggaagcatgg ctagaaagct accaagataa ggaaccagat
    13141 ttaaaatacc agcagtaacc cccagcgtaa ccgcatagcg tagaccaata atcttgaaga
    13201 agatgataaa cattactgct acaataatag ccactgtcac ttgccctcga acatagttgg
    13261 acaactgttg attcacatct gatagaactt gtccaacagg ttccttcaat tttcttggaa
    13321 tgaattgggt caaatagtta cgcaagcctt tcccatcacg caagagataa aagagcatga
    13381 aaggaacgat aatcaaggca acaatcactt gagaagcccc gctaataaag gcactcaccc
    13441 agttgactgc ctgagatgaa accttacttg ccaaaactgt agcctggcta gaaaaattgg
    13501 tcaaaacttg ctctaattga ggtctgaaat catctggcag atgctgggct accaatccat
    13561 taacaatcct atctatatct tctaagtaaa caggaacgtt tcttgcaaag gtcaaaacct
    13621 gacgttgcag atttggaatg gcgactgcca agccccaaat gataaagaga gcgatgataa
    13681 caaagacaat agtgatagct ataacacgat taaccttatg cttctccatc caatcaacaa
    13741 taggattcaa caaataatat aacaaaccag acaaaatgac tggcaacatc acaactgcta
    13801 aaaagtctaa aacaggtgaa aatagaaaac taatcttact taaaataaaa agattcagtc
    13861 ccaataataa ggttactaaa aataccgtaa ttgccttgtt atctaaaaac cacttgaaaa
    13921 accaagatag gctaaaatgt ttctcttttt gctccataaa tacttccttt ctattgttct
    13981 ctcattatac catttttgaa acaaaataac tcttttttaa agtgcttaca tctaattgga
    14041 tatctttaat ttacttgttt ttgtgatata atagtcttat ctttacacag aggtatagta
    14101 atgaaagaaa ctgtttattt tggaacttat acacgtcgta cttctcaagg gatttacaag
    14161 gcagactttg atacagaaac tggtcagctt tcaaatctag aactttttgc agctgagcca
    14221 agtccaacct accttgcctt tgaccagcac caacatttat acactgttgg tagccaagac
    14281 gataaggggg gaattgcagc ctatcaaact gacgggactg tgttaaatca tgttgttgaa
    14341 gaaggagctc cccactgtta tgttgctgtc gatgaaaagc gtgatttggt ttacgcagct
    14401 aactatcaca agggacaagt ccttgtttat aaacgccagg aagatggtag tcttctactt
    14461 agtgatatgg atcaacacag tggccaaggt ccacatgaaa atcaagcttc cccccatgtt
    14521 cactatacag atttaacacc tgaccactat ctagtgacct gcgacttggg aactgaccaa
    14581 gtcatcacct atgacctcga tcaagaagga aaattatcta agctctatac ctatcacagc
    14641 aagccaggag caggctcacg ccatatcatt ttccataacc actataaaat cgcttatctc
    14701 atttgtgaac tcaatagtac tatcgaagtt ttaatctacg atggcgttgg cgaatttgaa
    14761 cgtatgcagg tcatttcaac tttaccggaa gcttacgaag gctttaatgg aaccgctgct
    14821 attcatctct ctaaagacgg taaatacctc tacgcttcta accgtggcca tgattctatc
    14881 gcagtatata ccatccttgc ggacggtagc ttagagttat tagaaatcgt tccaacgcat
    14941 ggtcagactc cacgtgattt tgatttgaca cccgaccaaa aatttctcat tgttgtccat
    15001 caagactctg acaatgcaac tgtctttaaa cgtaattgtg acaatggtcg tctagcagaa
    15061 ctctccaacg acttccatgt tcccgaagca gtctgcatcc gttttgctcc ttaaaacacc
    15121 ataaaaaatg aacttggtat tttcaagttc attttttctt atagtctatt tccgacatta
    15181 atacggttaa tagcacgttg caaagcaatc ttagcacgac gttcttggtc aatcaaatgt
    15241 ttgtcttgtg cttcttcaat tgcacgttct gcacgaagtt tggcacgttc tgcacgactg
    15301 atatcgatat cacgagcacg ttctgcagag tcagcgacga ttgtgatcat atcattagca
    15361 atttcaataa cgccaccgtt tactgcaatc cagttcacgt gatctttatc atcgatacgt
    15421 tttaccttta cttcatcaac tgctaaaacc gcaatcatat tttcatgtcg tggcaagatc
    15481 cccatctcac catccagagt tcgaaccgat acatagctgg catggtgatc atagacgaga
    15541 ccatctggtg tcacgatctg gacagttaac tgagccatag atcacctctt aaaatcccat
    15601 tttttcagct tttgcaatca catcttcgat agaacctaca ccacggaagg catcttctgg
    15661 caagtggtcg tatttaccat caaggatttc cttaaagcca cgtacagttt cagcaactgg
    15721 aacataagaa cctggctgac cagtaaattg ttccgcaacg ttgaagtttt gtgacaagaa
    15781 gaactggata cgacgggcgc gagcaaccaa ggtcttttct tcatcagaaa gctcatccat
    15841 accaaggata gcaatgatat cttgcaattc atggtaacgt tgaaggacac gttttacttc
    15901 agcagcaact gcatagtgct cttctccaac gatttcaggt gccaaggcac gtgagcttga
    15961 agcaagtggg tcaacggctg ggtagatacc caattgtacc aacttacgtt ccaagtttgt
    16021 tgttgaatcc aagtgagcga aggctgttgc tggcgctggg tcagtatagt catccgctgg
    16081 cacatagata gcctggatag aggttacaga acccttcttg gttgatgtga tacgttcttg
    16141 caattgaccc atttccgtag caagtgttgg ttggtaacca acggctgatg gcatacgacc
    16201 caaaagggca gatacttctg aaccagcctg agtgaaacgg aagatattat cgataaagag
    16261 aagcacgtct tggccttcca catcacggaa gtattcagcg attgtcaaac cagtaagggc
    16321 aacacgcata cgtgctcctg gtggctcatt catctgacca aagaccatgg ctgttttctc
    16381 gataacgcct gattctttca tttcccagta aaggtcattc ccctcacgag tacgttcccc
    16441 aacaccagta aatactgaaa taccaccgtg ctcttgggca atgttgtgaa tcaattcttg
    16501 gattaagacg gttttaccaa ctccggcacc accgaaaagt ccaactttac cacctttaag
    16561 gtaaggggca agaaggtcaa taaccttgat ccctgtttca aggatttcag aagaggtaga
    16621 caactcatca aaagttggag cttttttatg aattggctga cgctctgcgt cttctgtaaa
    16681 aggagcttcc aaatcaatgg tatctcccaa aacgttgaag acacgtccca aagtttcttt
    16741 acctactggt acagagattg gacgacctgt gtccaatact tccattccac gagtcaaccc
    16801 atctgttgat tccatggcga tagtacgaac cataccatct cctaactcca aggctacttc
    16861 aaggacgatt tttgtttttc tttcgtcatt tttgtagacg acaagtgcat tgttaatctc
    16921 aggaagtttt tcccctgctg caaacaaaac gtctacaacg ggaccgataa cctgagcaat
    16981 tttacctgaa ctcatctcct tctcctattc tatataagat actgtcggtt cctagctcaa
    17041 ctaggtccta agttcatttt catacgagct ggactagagc ctattctaag gcactagcac
    17101 ctgctacgat ttctgtaatt tcttgtgtaa tcgccgcctg tctggcacgg ttatactgaa
    17161 ttgtcaaatc attgatgact ttcttagcat tatctgtcgc tgtttgcatg gctgtcatac
    17221 ccgcagcatt ctcagctgtc ttggcatcga taatggcacc gtaaatcata ctttctgcaa
    17281 actgaggcaa caactgctcc agaatttctt ctcggctggt ttccaattca aaagtcaagc
    17341 tgtactcttc atccgcttca tttggatcca agtcaacaat cggaagcatt tgttccacac
    17401 gcatttgact ggttagcgta ttgacatggt ggttgtagca aacataaagc tcatcaaaga
    17461 gttcattttg gtacatttca acagtttttg aaataatctt acgaacttga tcaaagctag
    17521 gttggtctgc caagccacgt aattcataaa gtggttgaat accgcgagcc ttaaagaaat
    17581 cagctcccat cccaccgata cagatcattt caaaaccttt accgtctggg tggtattctt
    17641 ctttcaactc cataacagct ttcaaaatag aggaattata acctccaacc aaaccgcggt
    17701 ctgaagtgat aacgatatag cctgtcttct tcacagaacg gctaatcaac atcggattag
    17761 ttgaagcacc agctccatta ccatgaagga tatctgtcaa aagtttacgc actttctgag
    17821 cgtaaacttg gaagttgcga gcagcttctt cagaacgacc tagcttagca gccgatacca
    17881 tttgcatggc attagtgatt tgactcgtat tttttgttga ggcgattttt gttttaatat
    17941 catttagaga tactgccatc tgacacctct attcttattg gaagctagat tgattgagaa
    18001 actctgtaat cgcagcatcc aagactgctt cttctggcaa gtcttttgta tcacgaatgg
    18061 tttccaaaat ctctggatgt tgagcatcaa agaaggtatg gaactcttcc tcgaaacgaa
    18121 caatatcatc tactggaaca gtatccaaga aaccatgtgt caaagcataa agaatggtta
    18181 cttgtttctc aacaggtaat ggtttgtgaa caggttgttt caagacctca acggtacgac
    18241 gtccacggtt caacttagcc tgtgttgctg cgtccaagtc agaaccaaac ttagtaaagg
    18301 cttccaactc acggtatgaa gcaaggtcga tacgaagtgt accagcaacc ttcttcatgg
    18361 ctttgatttg tgcagaacca cctacacgag atacagatga acccgcatcg atggctggac
    18421 gaatacctgc attgaagagg ccatcgccaa ggaagatttg tccatcagtg atagaaatca
    18481 cgttggttgc gatataggct gagatatctc ctgcttgtgt ctcgataaat ggtagggctg
    18541 taattgatcc accaccaagt tcatcagaaa ctttagctga gcgctcaagc aaacggctgt
    18601 ggagatagaa aacatcccct gggaaggctt cacgacctgg aggacgacga agcaagagcg
    18661 atagttcacg ataagctacc gcttgttttg atagatcatc gtatacaatc aaaacatgct
    18721 taccttgata cataaattct tccgccatag caaccccagt ataaggagct aggaagagca
    18781 atggagatgg ttgtgaagca gaggctgtca caacgattgt gtagtccaag gcaccgtact
    18841 gacgaagtgt ttctacttgc gtacgaactg ttgattcttt ttgtccaatc gcgacgtaga
    18901 tacagatcat atcttgatct ttttggttca agattgtatc aatcgcaatg gttgttttcc
    18961 ctgtctgacg gtcaccgata atcaactcac gttgaccacg accaatcggt acaagggcgt
    19021 caatagcttt caaaccagtt tgcaatggtt ctgaaacaga cttacgttgc ataacaccag
    19081 gagctggtgc ttctactgga cgagttttat cagtgtggat ttctccaaga ccgtcaactg
    19141 gacgaccaag cggatccaca acacgaccaa tcagactttc acctacaggg acttccatga
    19201 ttttccctgt acggcggatt gtatcgcctt cacggatatc tgtaaagtca cctaggatga
    19261 taataccaac gtctgttgac tccaagtttt gagccatacc ataagagccg ttttcaaaat
    19321 tcaacaactc tccactcatg acattttcaa ggccatgagc acgcgcgata ccgtccccga
    19381 tataggttac aacacctgtt tcagtcacat caaaattggg tttgaaattt tcaatttgtt
    19441 gcttaattaa agcgctgatt tcttgtgcgt taattgccaa aagaacacca ctttctattt
    19501 caaattttct ttaacaactt taagttgttg tttaatactc acatcaattg tcttgtgatt
    19561 ggcaaaaatg acaaaaccac caatgagact ttcatcgatt tgttctttta cactccttac
    19621 tttcagagac atttttttct caatcaaagg gagcaaacga gtcttctgtt catcagttag
    19681 aggatgagca gacgtaatcg tcacttcaaa tcgatttgtt tctttttcaa gtcggttcaa
    19741 gcaatctaca agcacatcat aaaaaagatt tgctctgtga ttgtaggcca gaacctggat
    19801 aaagttttgt aataaaggcg acacagaatc ttggaaaaaa gcaattgttt tttccttatc
    19861 cgactcgtct actgccactt gttttaaaaa agaaggcaga cctgtttttt caacaacttg
    19921 cttgatttga gtcaagtctg aaaagatacg gtcttcttct cctttttcaa gtaccaattg
    19981 gacaaaaggc atgctgtatt tttcaattac ctttactgtt ttcttgtcca ttaagcttct
    20041 cctagctgat cgatatactg atcaatgagt gctttatggg catgactgtc aaggttttgt
    20101 gagatgattt taccagctaa gctgatggtc aaatctgcga cctcaccctt aacactctgt
    20161 aaagcttcta ctttattttg agcaatttct tgattggctt tttcttttaa gtgtcctgct
    20221 tctagtttag catctgctaa gatattagcc ttactttgct cagctgtttc ctttgcattt
    20281 tcaatgattg tcttagcttc tttacggcta ccagccaatt catcttcgcg tttttgagcc
    20341 aatacttctg ctttttgacg ggcttcttca gctctgtcaa tatctgaagc aattttttca
    20401 gctctttctt cgaaaatgcc tgtaatatta gaccatgcaa attttttaat caagactagc
    20461 aaaagaataa aagagccagt gattaaaata aaattaccaa ttaattcacc tactgttacg
    20521 tgcatcagtt actcctttct attcctcttc aatattaatc ttattcccta aatacacaga
    20581 tgtcaaaaga gtaaaaacat aagcttggat gcaggaaata aagacagaaa atgcagtcca
    20641 agccaaatta gctccaaagg ctactggata ccaataaata gcttggtggg aaagaagaag
    20701 taacaaactc gtcatgacct ctcctgcaaa gatattccca aaaatccgca aagccaaaga
    20761 taagaagttt gtaaattctt ccaagatatt catcggtatg acaaaaacag gactcataaa
    20821 agattttata ctttttttaa atccacgacg acgaacgctt tctatatgtg tcaacaaaat
    20881 gacaagaaaa gataaggtta agtcatacgg taaattagcg gttggcgaac tccaccagtt
    20941 agtcccatcg atcgtttgaa gctttgtcat taagccaagg ttattggcaa tcaccatgaa
    21001 aaggaataaa caaaggaaaa agagtgagta atctttcatg tagcgcgaac caatgttagg
    21061 ttctgtaaat ccaataacaa agtcatagac atactcaagt acattttgct ttcctttggg
    21121 tttcaaggtc atattgcggc ttgcccaata aataaagaca aaaataactc ccacaatcaa
    21181 caaagtcatg gctaacatag tcagattgaa gataacagga ccaatagaga tgattggatt
    21241 aatactttct tccatctaaa atcctccctt ttcttctcgt tccatgtatt tatttgataa
    21301 taaatgagaa gacaagagtt acaaagaaag ttccttcaat aaaggcaaca cctaaaaaca
    21361 tcaaactacg aaactcagaa agcatatctg gttggcgtgc tactgattta aacagtccat
    21421 tcatcaataa accttcaccg acagatacgc ccatacaggc aatacataag cctaaaaatg
    21481 ttaaattcat aacaaattct cctttaaaat aaaatttact tacttagtgt agtcctaaaa
    21541 tttaaatttg tcaactttta accgctattg gaagcgttta ataaaaatta tttccattt
//
LOCUS       DAYYQN010000035        21218 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYYQN010000035 DAYYQN010000000
VERSION     DAYYQN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.035"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(55..924)
                     /gene="rsmI"
                     /locus_tag="ACHBRQ_001288"
     CDS             complement(55..924)
                     /gene="rsmI"
                     /locus_tag="ACHBRQ_001288"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9592956.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(927..1244)
                     /gene="yabA"
                     /locus_tag="ACHBRQ_001289"
     CDS             complement(927..1244)
                     /gene="yabA"
                     /locus_tag="ACHBRQ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9592957.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(1284..2174)
                     /locus_tag="ACHBRQ_001290"
     CDS             complement(1284..2174)
                     /locus_tag="ACHBRQ_001290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9592958.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(2171..2809)
                     /gene="tmk"
                     /locus_tag="ACHBRQ_001291"
     CDS             complement(2171..2809)
                     /gene="tmk"
                     /locus_tag="ACHBRQ_001291"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9592959.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(3029..3826)
                     /gene="proC"
                     /locus_tag="ACHBRQ_001292"
     CDS             complement(3029..3826)
                     /gene="proC"
                     /locus_tag="ACHBRQ_001292"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9592960.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(3830..5092)
                     /locus_tag="ACHBRQ_001293"
     CDS             complement(3830..5092)
                     /locus_tag="ACHBRQ_001293"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9592961.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(5102..6211)
                     /gene="proB"
                     /locus_tag="ACHBRQ_001294"
     CDS             complement(5102..6211)
                     /gene="proB"
                     /locus_tag="ACHBRQ_001294"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9592962.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(6312..8195)
                     /gene="cbpE"
                     /locus_tag="ACHBRQ_001295"
                     /gene_synonym="pce"
     CDS             complement(6312..8195)
                     /gene="cbpE"
                     /locus_tag="ACHBRQ_001295"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HFM9592963.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(8198..9085)
                     /locus_tag="ACHBRQ_001296"
     CDS             complement(8198..9085)
                     /locus_tag="ACHBRQ_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9592964.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(9075..9536)
                     /gene="lspA"
                     /locus_tag="ACHBRQ_001297"
     CDS             complement(9075..9536)
                     /gene="lspA"
                     /locus_tag="ACHBRQ_001297"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9592965.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAILKEEINGN"
     gene            complement(9533..10441)
                     /locus_tag="ACHBRQ_001298"
     CDS             complement(9533..10441)
                     /locus_tag="ACHBRQ_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9592966.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            10470..10631
                     /locus_tag="ACHBRQ_001299"
     CDS             10470..10631
                     /locus_tag="ACHBRQ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9592967.1"
                     /translation="MIPYFRSFPKRKKEHPLLLNYLHYLPFSSQPYFQNQFKKITGYT
                     PLQYRKENR"
     gene            complement(10683..11354)
                     /locus_tag="ACHBRQ_001300"
     CDS             complement(10683..11354)
                     /locus_tag="ACHBRQ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9592968.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(11408..11629)
                     /locus_tag="ACHBRQ_001301"
     CDS             complement(11408..11629)
                     /locus_tag="ACHBRQ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HFM9592969.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            11901..12710
                     /locus_tag="ACHBRQ_001302"
     CDS             11901..12710
                     /locus_tag="ACHBRQ_001302"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9592970.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(12891..13766)
                     /gene="aguB"
                     /locus_tag="ACHBRQ_001303"
     CDS             complement(12891..13766)
                     /gene="aguB"
                     /locus_tag="ACHBRQ_001303"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HFM9592971.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(13776..14861)
                     /gene="aguA"
                     /locus_tag="ACHBRQ_001304"
     CDS             complement(13776..14861)
                     /gene="aguA"
                     /locus_tag="ACHBRQ_001304"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HFM9592972.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPVVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(14858..15985)
                     /gene="nspC"
                     /locus_tag="ACHBRQ_001305"
     CDS             complement(14858..15985)
                     /gene="nspC"
                     /locus_tag="ACHBRQ_001305"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HFM9592973.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(15985..17244)
                     /locus_tag="ACHBRQ_001306"
     CDS             complement(15985..17244)
                     /locus_tag="ACHBRQ_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HFM9592974.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(17241..18101)
                     /gene="speE"
                     /locus_tag="ACHBRQ_001307"
     CDS             complement(17241..18101)
                     /gene="speE"
                     /locus_tag="ACHBRQ_001307"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFM9592975.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            18357..18961
                     /locus_tag="ACHBRQ_001308"
                     /pseudo
     CDS             18357..18961
                     /locus_tag="ACHBRQ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18941..19066
                     /locus_tag="ACHBRQ_001309"
                     /pseudo
     CDS             18941..19066
                     /locus_tag="ACHBRQ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19038..20513)
                     /locus_tag="ACHBRQ_001310"
     CDS             complement(19038..20513)
                     /locus_tag="ACHBRQ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9592976.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSARKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         6189 a         4904 c         3854 g         6271 t
ORIGIN      
        1 catcactaat taagttaaac agaaacagac agaattatta catcctgtct ccctttattg
       61 tttttcttcc cagtcgtggt aggcagcgta gagttgactc ttattccact ggtaaatctt
      121 agctacttcc ttaatagctt gatttttctt cataccttgc tgaatacgag cttggatttc
      181 taagaacaag tcttcctcgc ctttttcttc cacatcctga ctggcacctt caacaatcag
      241 aagacattca cccttgagag acgtttcagc aatgctttcc agcaattcag aaattgtacc
      301 tctttggtat tcttcataga ttttggtcaa ttccctgacc aagacaacgg agcggtcacc
      361 gtagacttct agcatatttt ccaatgtatc tgccacacga tggggcgatt cataaaaaat
      421 ctgagtttca ggatagtctt ttttcgagtc gaaaaattgc ttttgctggc ctgattttct
      481 cggtaaaaag ccgtaaaaga tatgtggctg cggcgctaaa ccactggcaa tcaaggcgga
      541 aattcctgca gaggcacctg gaactgtaac aactgcaatt tcttcctcaa tagctgcctt
      601 aaccaaatca tgaccagggt ctgaaatgct aggcaaaccg gcatcagaga cctgagcaat
      661 actttgccct gctttcaaga aaccaatcaa atcaggaatt ttttccttgg cattgtgctc
      721 atgaaaactg atctgcttgg tggaaatgtc aaaatgcttg agcaaaagcc ctgtattgcg
      781 cgtatcctca gcagcaatcc agtccacttc tttcaaggtc tggatagcac gaaaagtcat
      841 atcatctaga ttgccaatcg gcgttgccac tagatacagc ttgccatagg gagactgccc
      901 cttaaaactt ttttgaattt gcatgcttac tccctgtata acaactcatc acagaacata
      961 cattcttcgt cctgctctcg acgttgtcca taaaaatcat tacatacgtg aaatccatca
     1021 cggtaaatac gacggacact ttcgcgaaca tgcttggcct tgacgggagc gtctgcttcc
     1081 acctcaccca agcgttctcg caacttacta ttttccaagc gaagagctgt attttcttct
     1141 accaagctct tgagattttt cttgatggct tccacatcgg ctaaggttac caataattgt
     1201 tgggaaaaat catccagcgc gtcaaataat tcttttttgt ccataaaaca gccctttcct
     1261 ttctttatca ttcatttttg agtttatatt tctttcaaga ccagatattc catggcattt
     1321 tgaaagctga cattagcttg ccacattttt ctagcttcta gtaaatcttg tagaatcact
     1381 cttactctta cctgcaagag gtcctgccca cagagaactt caagaatccg taaaacctga
     1441 tcctgttttt ccttatcatc tgccaagttg gctaatttgg caacctgtag ataactttct
     1501 tttttcttag ctactaacca agtcagcagg cgttcacttt catcgaccaa ggtccaaaaa
     1561 cttgcctgat tagccaactt ttctgcttca gctcgcgatt gactaaactt agctaaaaga
     1621 gtcgcttttt tcttaacaag tcccatttgt tctaagagta agataagttt ttcttcttgc
     1681 tttttaaagt ggaagatctg agtccgactt cggattgtcg gtaacatctt ttcctcatcg
     1741 ctagtcaaga agaaaatata aacttcactc tggggttctt cgatgacctt gagcagagaa
     1801 ttggctgcgt tgggatgcat tttatccgct tgctcgataa taaagacctg ttgctggctt
     1861 tcaatccctg cttgagaaaa ctgtcccacc aattcccgaa tgcgttctgt cttgatgacc
     1921 tgattgactg gcttaatcaa ggtgacatct ggaaactctt cctgttcaat cagcttgcaa
     1981 cttcggcatt tctcacatgg taagacgcca actttatccg tacaaaagag gctcttagct
     2041 aaaaattgcg ccatttccaa gcttccaaag aaacctgaaa agagataggc gtgattgagc
     2101 tggtcttgtt ctaagatacg gacaaaacgg tcaaactgag ctggttgcca agcctttagt
     2161 tgatcttgtt tcatttggcc aagcccattc cgtcaaacaa gacagccttg gtagtttcca
     2221 caacttgctc caaagggaga ctagcatcaa tcttgacaat gcgatttccc tctttatcca
     2281 gaagagaaag gtagccttga cgaacttttt tatgcaagtc caacccttcc aaatccaaac
     2341 gattaacctc gcggtcacta ttcgcagcaa tacgagccag cccttcttcc acctcgatgt
     2401 caaaatagag tgtcaaatcg ggtttgaggc catctgtcgc aaactgattg agccagtcaa
     2461 tggcttcaat atctaagcca cgaccaaatc cctgataggc aacagaacta tcgataaaac
     2521 gatccatgat gaccaacttg ccagcttcaa gggctggaag aactttttcc accaaatgct
     2581 gtctgcgact ggcaatatag agaagtagct ctgttttagc atccatctga gtatgacttg
     2641 gatccaaaat cacttcccga atcttctccc caatcaagac tccgccaggt tcacgggtcg
     2701 tcaacacctc tactcctttt tcctttaaaa ttggtagcag agcctctaaa acactggtct
     2761 tgcctgctcc ctctggtccc tcaagagaga ctaaaaatcc ttttgacatg tctaactcat
     2821 ttctttttta ctacttctat tctatcaaaa aaataggggt ttgtgacaat ctttttgtct
     2881 tctcatttca tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgtac
     2941 ttgagtacgg caaggtgaag ctgacgcagt ttgaatttga ttttcgaaga gtatcaacca
     3001 ccataaaaga ggcagataaa actacctctt atttacctag ttcttgtgtt cgtttatagg
     3061 cttgatgaac tgcctccatg actgtgcctc gaaaagcatg cgcttctagg cttgctacgc
     3121 cagcaatagt cgaaccgcct ggactacaaa cttgatcttt caaaactcca ggatgttgct
     3181 ggctttctag gaccaattgc ccagctccta ccacagtttg ggctgccatt ttcaaggctg
     3241 tttctcgtgg taatcccgtc tgaacacctg catctgccaa ggcctcaata aagagataga
     3301 caaaggctgg tccacaacct gcaagacctg tcgctgcatt gattaagctt tctcctaatt
     3361 caaccaagag accagccttg gctaaaagct gacaaaagat ctcactgtcc tcagccctgc
     3421 aattaggaga caaggcataa ctaatcactc cttgcccgat agaagcaggg gtattaggca
     3481 tcatacgaat aattcggtgt tggcttggga taagacttgc tagtttttct aaggtcaatc
     3541 cagctgccat cgaaatcaaa agaagacttt ctcttttttc aaggatggtc tgatattgag
     3601 aaagcagttc agaaaactga gcaggcttaa ctcctagaaa aatcacatct gcttctgcaa
     3661 acatttcttc attgctggaa gcctgaccac caaagtctgc aatgaaagca tctaccttag
     3721 cttgactacg attggcaagg agaatctcat ctgacgtcct agtctgcaag acagattttg
     3781 ccaagctagc acccatattc cccaaaccga taaatccaat cttcatctct tactccctta
     3841 tctgcccatc accagcaacc acatacttgt agctggtcaa ctctttcaag cccattggac
     3901 cacgcgcgtg caatttctga gtagaaatcc ccatttcaca accaagacca aattgtcctc
     3961 catctgtgaa acgagttgag gcattaacat acaccgctgc agagtccact tgatctgtaa
     4021 agtatgctgc agcttcagca ttttccgtca caatagcatc cgaatgatgg gtactgtggg
     4081 attcaatatg cgcaaccgct tcttctaaac tgctcacaac cttaacagct agaatatagt
     4141 ctaaaaactc ggtatcaaag tcttgtgctt gagcagcttg acctgaaaca aactggcttg
     4201 ctttgctatc taggcggaat tgaattggtt ccaacccagc ttcttttcga tctgcaacca
     4261 gcatttgatc caagcgagga aggatgcttg ctgccttgtt ttcatgaacc agcagaacct
     4321 ccatggcatt acaaacagaa ggacgactgg ttttagcatt gttgatgata gacagcgcct
     4381 tgtcttcgtc tgcatcctta tccacataga catggacaat cccagtccct gtctcgataa
     4441 caggtacaat cgcattctca accactgcat tgatcaagcc agctcctcca cgaggaatga
     4501 gaaggtctag atagcccttg gccttcatca tagcataact actttcacgg ctagtatcct
     4561 ccaccagttg aatcacattt ggatgaatag tagtcgtctc caagcccttc ttcaaggctg
     4621 tgacaatggc atgggttgtt tgataggcat ccttaccact acgaagaaca accgcatttc
     4681 cactcttaag agtcaaagca gccgcatcag acgtcacatt tggacggctt tcatagataa
     4741 taccgatgac acccatagct acacgttttt ttgtgataac caaaccattt tcaagctgac
     4801 ttgtttctaa aacttcaccg attggatctg gtaaggcaac cacttcacga attcctcttg
     4861 ccatcgcttc tatacgatct gcatccaaat aaagacgatc caacatcaca tctgagattt
     4921 tccccttagc cgctgccata tcgagggcat tagccgctaa aatttcctca gtagcagcca
     4981 ctaagtgatc agccatggct agcaaggctt ggtttttcac ttcttcacta gctgtgttaa
     5041 tcgatttttt aacagcctgt acctgttcaa attgttcttg tctactcacc atagtttacc
     5101 tctaaaattc tgtaaaaagt agttggattt caggagtaat ggaaatccag tcgtcacggt
     5161 aaatcaagac acccttggct ttttgagaac gcaacatatc ctccaaagca gatgctccaa
     5221 attgcacgcg tccttttcca agtgattttc cactttcctt atcaaatact gtcacgatat
     5281 caccgtaaga aaagactcct tctgcttcaa cgataccaga taagagaaga ctctttccat
     5341 gttgagagag agcttccgca gcccctttat caacccaaat agaaccttga ctctgagcat
     5401 agaaggcaag ccattgtttc tgggtacgaa gccccttctc ttgagcaaca aagtaagaac
     5461 catcctcggt ctcctctgcc gcctcaatca tggaatctga tttcaaggat gagcagatat
     5521 aaacaggaac tcctgattcc gtcgcgatag ttgcagcctt gattttggtt aacatacccc
     5581 cagttccgtt tgacgaacca gctccaccag ccatatcaat aatctcacga ttgatggtct
     5641 cgattctctc caagcgtttg gctcttggat ctgaattagg atttccagta tagagaccgt
     5701 ccacatctgt caagaaaact aaaaggtctg cttggaccat cgccgctact tgagcactta
     5761 gagtgtcatt gtccccaacc ttgagctcat caataacgac actatcattc tcattgatga
     5821 taggaattgc cccacggttg agcaaaaccg acaaagcctg atgggcattt ttataacgac
     5881 gcttatccac aaagtcatct tgggtcagca agatttgtgc agaaacgatt tgacgcaaga
     5941 gaagattggt cgtatattct tccaacaaaa gtccctgccc taccgctgct gaagcctgtt
     6001 tatcagcaat tttagtcgga cgctttttaa atcctaaggc tccaaaacca gccgcaatgg
     6061 cacctgaaga caccaaaatc aactcatgac cagcctcgtg cagcatagcc aactgctggg
     6121 taatatcctt taccttacta cgtgataaac ttccatcctc atttgtcaga gaagaagtac
     6181 ccaccttaaa gacaatccgt ttgtatttca tagtctcact ccgattattc atatttatcc
     6241 attataacat gaataacagg aaatagaaaa gagttgatag gaaaaagaag gccctaaaac
     6301 cttcttttct actactgttc tgattccgat ttgtttacac tcgtttcaga gctttgtgaa
     6361 gttgaggatg tcaaatcttg actagttgaa gaatttgtaa cactttcatg ttgactcgtt
     6421 tctttatttt caagatcctt tttcactact tctttggatt cttttatttc tgaatgactt
     6481 gtagttttaa ttgtagcaga tttctcgatc ggaacatcca ctaaccaggc accacttgaa
     6541 tccacagtat agccttctgg tgtcttacca ttcacaagta attgaccatt ttctttcaag
     6601 aagtaccatt tatccttgtc tttaatccaa cctatagctc gtgaaccatc tgtcttgtag
     6661 aaataccaat catttgcctt tttaagccag ccttgcttca tagctcctga ttcttctaga
     6721 tagtaatgat gtccagcaac ttctatctca cctgtcttca tgataccagt agaatccata
     6781 tagtaccagg cttctttatc ttttacccag cccgttttca tttcccctga tggagcaaag
     6841 taataccatt gccccttata ttgaagccaa cctgtcttca tagagccatc aacatccaaa
     6901 taataccact gctctttatc ttgaatccaa ccaatttcca tctggttttc tttgttaaaa
     6961 taataccaga ttccagcgat tttcttccaa tttttagcag caacaccaga gtctgtcaaa
     7021 tagaaccaac gattgttcca ttttttccat tgattctgta ataagatacc cgtttggtta
     7081 aagtaatacc attcaccctt aatttcattc caaccgacag catattctcc tgtagaatca
     7141 ggcgcttgat accaccagtt tccatatgaa ctcttatgcc aaccagcttg aaaacttgga
     7201 atcggcttgt aggaagttga aatgtttact aaaccgtctt gtcgaatatc aaaaactgtt
     7261 gcatcatagt ctttgctggc tgcattgatt ctctcaattc ctcgttcttt gagccaatta
     7321 acatactcac tatcaacacc atttttccaa ggtagactat ccgaagtttg aacaatcaaa
     7381 ctcggactca aatttttaat gaaatccttg gtatttgatt tgttggtatc atggtgatga
     7441 ttaaacttca tcaaatcaac ttttccaatg agaggaccat acttgtcttc tgctccatga
     7501 acattatcta aatcgccccc aaggtaaatt ttcttgccat tgactttcac cacgctaatc
     7561 aaggaattgg aattgtcatc ccaaattttc tttaattcac ccgatgaatc agtttcattt
     7621 tcataattat agagctgaat atccatgtcc ccaaactgaa aatgagcatc cccttgtgtg
     7681 atattttgaa taactgaaac acctttttct gcagcagtct gtaaaacctt atcatagcca
     7741 tacagattat cccatagacg ttcagaatta gtaatacgac tatcactata tttcttaaga
     7801 tagactcggt caactggata ggtagacagt aattcatcaa catttccaat atgatcactg
     7861 tgggtatggg tcaccaaaat aaaatcaagt ttttggacac ccaattcctt caaacgacga
     7921 aagacacggt ctgttagaac atgcttataa gacgtttcaa ttccttctct ccatggatag
     7981 cgagaatcac ttccatctgg gaaatcataa tcttctcctg tatccaccat ggcaaaatgt
     8041 ccattgcttt caagaataat cgcatcactg ccaccttctt gaacattgat aaagtggatt
     8101 ttatttcctg aactttcttg agcctgaaca ttcccatacc atgacaaacc taaaaaagcg
     8161 cctacaagtg ctaaactagt taatttcttt ttcattctta ctttctcaat ctctccaagg
     8221 tttccttaaa aatctctggg atatctgctt taaattccaa ggtcttacct gttctcggat
     8281 gagtaaaacc taaagtcttg gcatgaagaa attgtccatg tcctttcaaa gtcttgcgag
     8341 gaccatagac ctcatcacca gcgactgaat ggccgatata agccatgtgg acacggattt
     8401 gatgagtgcg ccctgtctcc agttgcaact ctactaagct ataatcgcca aagcgttcca
     8461 agacgtgaaa acgcgtcact gcaggcttcc ctttagcagt tacagcctgt ttcttacggt
     8521 ctttttcact ccggccaatc ggcgcttcaa ttacaccacg atcattaggc agatttccat
     8581 gaacaatcgc ccaatatttg cggagagact ttttatcttt gagttcttgg gcaagtacta
     8641 gatgcgcatc atcgttttta gcaatcatga gaagacctga cgtatcctta tcaatacggt
     8701 gaacaatccc tggacgcaga accccattga tacccgacaa gtccttaata tgatacatga
     8761 gggcatttac tagggttcca ctggtatgac cagcactcgg gtgcacaacc attccctgag
     8821 gtttgttaac gacagccaca tcctcatctt ggtagactat ttctagcgga agatcctcag
     8881 ccacatactc taatacctct ggttctggca catggtaagt gacgacatca ccctcttgga
     8941 ctgtgtattt agctttcttg acttgaccat tgaccaagac ctggcctgat ttaatttgtt
     9001 cattcgcgag actacgtgat aattctgaca aatctgacaa agccttatcc aaacgcagac
     9061 caccagtttc aattttaatt tccatttatt tcctctttta gtattgcaat caataaaata
     9121 atcactccaa ccgtcagata gctatctgcc acattgaaaa ttgcaaagtt gataaagtca
     9181 aggtggaaca tatccacaac aaagccctga ctgaccctgt caataaagtt tccaagacca
     9241 cccgcgatta ttagagtcaa acccaagacc atccagaatg agtcctccat gtgtttatgt
     9301 aaataccaaa tggcacctat cacgacaacc agagtaatga cagcgaataa cagctgctga
     9361 tcttgtaaga tagaaaaggc tgcacctcga ttttgcaggt aggtcaagct aacgaaattg
     9421 ggaatccagg agcgcacttc acccagtgga atctgctgga cgatatagga tttgaccaac
     9481 tgatccagcc caattaaaag cagtacaatg actgccacta ttgctctttt tttcatgatt
     9541 tcctcttttg atcaaaatat tcttgcatga cttctacgaa gagagtccca gcttgactaa
     9601 gctccacttc ttcacgttta acatagacca tgcggttatc taggttatcc ttgagacgaa
     9661 taactgtaat gccattaaca ctgtcactat ctaaaaatcc agaacctgtc gcataggcgt
     9721 ccgtccgctc caaaatacca ttcaaggtgg cacggtctgt cacattaaac atctgtgagc
     9781 tagcgctggt atcgacaaag ttctctgaat aataaaggta ctcgtctttc tcttgagtga
     9841 aacgaaccgt tggtaaatcc gctaaatcct ccatgactaa ttcctctttc tgggctaaag
     9901 gatgaccctc acggagataa atatgggtat ggaaaggaat caattcgatg acctccagac
     9961 ctaatttttc aacccgttgc ataatcccct ttttattttg attgttgagg tagataatcc
    10021 caatctcact atgcccttgc gccacttcat ctaatatttg aacagtagtt gattcaaaaa
    10081 tacggaagtt cttatagtca ggatagcgct ctgaaaaggc cgtaatagtt ggtggcaaga
    10141 agtcatagtg ctggctagca acagaaaatt catctttttc ttcttcagga ttggcatact
    10201 gattttgaaa aatatcaaat cctttaacca attcttgcgc tttttcataa aattccatcc
    10261 cacgacgggt caagaaagtc cctgagctgg tccgacggaa aatcttaaaa cccaactctt
    10321 tttccaaatc acgaacagaa atagacagac tcggctgact aacatacatc ttttcagcag
    10381 cttcacggaa agtaccacta ttggcaatgg ccacaacata gcgtaattgt tgaatgttca
    10441 tcttctaccc ctaacttctt tatcttttca ttataccata ttttagaagt tttccaaaaa
    10501 ggaaaaaaga acatcctctt cttcttaact atcttcacta tctgcctttc tcaagccaac
    10561 cttattttca aaatcaattc aaaaaaataa ctggatacac accactacaa tatcgaaaag
    10621 aaaaccgttg actatttgtg aaaaaagaaa atgccggaaa gttccgacat tattttagta
    10681 agctaacttc ctgaaaatag gagcaaccac acatcccaga aggcagtgat attaatgaga
    10741 atatgaacta gtattggata gtagagattt ttagtcatgc gatacaatag agctaaaata
    10801 agacctccac tagcataaat gaaaaaagca agaggagtta aagcaaaatt gatatgaata
    10861 taaccgaaaa taatagcaga aagcaaaaca tctccgtacc aaggtgagtt tttgaaaaag
    10921 gttgtcataa gcacacctcg ataaatcaat tcctcagcaa taggggcgat gaagcaaacg
    10981 atgagcaaga aataagggaa ctcctgtctc cccatcattt ctaccgtttc attcaaagaa
    11041 atctgatttg aagacaggga tatgaaatac gaaaagagga agtcagacat atatgaaatg
    11101 atgtagccca gtaaaaggta gatgaagtac ctcagctgcc acttgaaatg aaaaattttc
    11161 tttcctgcaa aaaccagtag atagatgacc gctaagaaaa gaactccgct ctccatcaaa
    11221 agcagaatct gaaaaaattc acgacttgct ggtagatagg gctttgcgag gtgattgaaa
    11281 actctccaaa accaagttga tttgtaaaaa attaaggaca atgctagtaa aatttgaata
    11341 gcccgttttt tcatattaaa ttctctgctt tctccacttg tttcttcata ttactaataa
    11401 attttactta aaatctcgca gcacttcctt tgcaaagatg attgcctcct ccaatatatc
    11461 ctaaatcata ggtgcctctg ggacaaaatc catgaaccgg cagagttaat actggttcta
    11521 tttcccaatc gtacctacca ttaggatttt taccatatga atctgctcca ccttgtatca
    11581 gctctaattc ttcatttgtt agatttatat tttttgtcat taaaatcatg atatatttcc
    11641 tccattattc ttcagattag ttgagtaatc tgatactcat acctacttac aaaaaaacta
    11701 ttatattaag ttggtttttt aatttgtcta gttgcaacat tgacaaacat agtatagcat
    11761 atctttgcca aatatcctct tcaaatcatg aattgtcatc aaaacatctt aaactataaa
    11821 atcaattagt ctcaagcttt ctatcaattt cttctccaaa atatgctata atactaccaa
    11881 aagataaaga aggaagacct atgattaaac tactagcctt ggatatggat ggaaccctcc
    11941 tcaatgaagc caaggaaatc ccacaagctc acattactgc tattcacaaa gccattgaaa
    12001 aaggtgtcaa actggttctc tgtacgggtc gccccctttt cggtgtcctc ccctactaca
    12061 aaaaactggg gcttgacctc cagaacgaat atgttattgt taacaacggt tgttcaactc
    12121 accaaaccag tgattggggt ctagttgact ggaaagaact gagtccagct gacatcgaat
    12181 acctctatga ccttgctgaa aagagtgatg ttcagttgac actttttgac gagtcacatt
    12241 attttgttct cggtggcaag cccaatcaag ttattgaaaa tgatgctaaa ctagtatttt
    12301 cagacctgac tgaaatttct cttgaggaag cgactagtgg aaagttccgg atgttccaag
    12361 gtatgttttt aggaacaaaa gaacaaacag acgattttga gcagcgtttt gctgaagagc
    12421 tttgccaacg attcagtgga gttcgttcgc agcctgtcat ttatgaagca atgccacttg
    12481 gaacgacaaa ggctactgct ctttcacgac tagctgagat tttgaagatt gattcctcag
    12541 agattatggc catgggcgat gctaataacg atatcgaaat gctccagttt gcagggcttg
    12601 ggattgcaat gggaaatgcc agcgattatg tcaaatctct tgcggatgcc gttacctcaa
    12661 gcaacgaaga agacggcgtt gcgcgtgcta ttgagaaata tattctataa aaaagaaaag
    12721 caaatagaca gaagttactg ctatttgctt ttttgctaat attttaaaat acggactaat
    12781 caatagacaa gaatagcgaa tcataaaaat cgatttacta gatgccatag tatcttatag
    12841 ttgctaataa gaagttagtc tagcagaatg aatctctcat ttctcccaca ctaatctgta
    12901 atttgtctat acatttctgg tcttctatct cgaaacaagc cccagtttag gcgttcactt
    12961 gctcccttgt ctaggtcata agtagctaac agaacagctt cttcttgtct ttcagctcgt
    13021 tctagaatag ctcctgtttc atccgtcata aaggaggaac cgtagaagtc aagactggaa
    13081 ctctgtccgc cattttcctc actaggagta acctcctcta aaccataacg attggctgcg
    13141 atgactggaa caatattcgc tgctgcgtgc ccttgcatag tacgttgcca gtgaccacaa
    13201 ctatctgtat ccaaaattgg ctctgaaccg atagctgtag gataaaagag caattcagca
    13261 ccattcaatg caagacagcg cgctgtttca gggaaccatt gatcccaaca gataccgata
    13321 ccaatcttag catagcgagt attccagacc ttgaaaccag tgttaccagg cgtgaaatag
    13381 aatttttctt gataataatg gtcatctggt atgtgggtct ttcgataaac gcccagcact
    13441 tccccatctg catcaatgac ggcaatagag ttatacaaga cattaccatc tttttcatag
    13501 aaactgattg gtaaaacaac ttgtagttcc ttagcaatca ccttaaaatg ctgaatggca
    13561 gtattttccg ctacagattg ggcatactgg tagtagtcat actgacgttc ctgacagaaa
    13621 tagggatgtt caaacaactc gggcaagaga ataatttggg ctccttgctc agcagcctga
    13681 cgtactaaac gctctgcggt ttggatattt gttgccacat ccttagcgca ttgcatctga
    13741 atggttgcaa ctcttacatt tctcatcttt ttctcctatt ctggaatttg ttgggtgata
    13801 cagtggatat taccaccacc taagagaata tctctggctg gtattccgac aactttacgg
    13861 tctgggaaac acttgctgag gatatctatg gccacttggt cgtttacatc ctcaaactgt
    13921 ggaaccaaga cagccttgtt ggcgatataa aagtttacgt aggaagctgc tagtcgttca
    13981 cctgcgtatc gctcttcttc tccttcttca tagatgtagc ctggcaaatc ttcttctgtc
    14041 acaacttgtc gaactacagg gataggcaat ttatgaatgg tgaagtgaca accttttgca
    14101 tctgtttcct gttctaagag ttcgagatct gcttttgaca tggcatactg gggatcattt
    14161 tcgtcatctg tccaagccaa aacaagctca gcaggaccaa caaaggcagc aacattatcg
    14221 acgtgttcat tggtttcatc ctgataaata ccataaggaa gccaaataac tttttcagca
    14281 ccaagacttt ctaataatgt gttttcaatc tcctctttag tcaagttagg attgcgacca
    14341 ggactaagca agcaactttc agttacgaga atagttcctt gaccatcgct atggattgcg
    14401 cctccttcca gtacaaaagg tttagcatca tagacaggcc tttccaaagc ctcagcaaaa
    14461 cgactggcta cttggtcatc ctcttcataa tcttgataaa gaccatcata ggtgcctccc
    14521 caagcattga aggcccaatc cacggctaat ttcttacctt tatcattgac gagaatggtt
    14581 gggccagtat cacgcgccca ggcatcattg gtgggaatgt ctaaataaac aaccttgtct
    14641 ccaagatagg attgggcttc agatagatag gcctgctcca ccaaaagata gactctttcc
    14701 ccttctgcta tggtctcgat gatctgagta aatgctcttt tagcagcctt tccttgaaaa
    14761 ggccatgatc ctggtcgagt cggccatatc atgagggtac catgatgggg ttcgtactct
    14821 gctggcatgt gatagcctaa tttttttgga ctgtccatca tgataatctc cctttaaagt
    14881 cttgatagcc aaaagctttg agtaagctac agtctccctg ttcgtccatg agatagagac
    14941 ttggcaatcc aataccatta aaggtattat ttttgacaaa agaataaatg gccatgtctt
    15001 gaaaataaag tctgtctccg atttggactg gattttcaaa actataatca ccaatcacat
    15061 cgccagtcag acaggtatta gaagaaagtc tgtaggtatg ggctttttcc tgtgcctcaa
    15121 agccatttct caaaggtgga cgatagggca tctcaagtac atcaggcata tggcaggtcg
    15181 cagaggcgtc taaaaccaag atttccatac cgttttctac aatatctaat acctcagttg
    15241 ctaaataacc cgcattaagc gcaatggctt caccaggctc gatatagatt tcaagattgt
    15301 aagtttttcg gatacgcttg atttctgaaa tcagcaaatc cacatcgtaa ccttctcttg
    15361 taatatgatg accaccaccc atattgagcc attttacctc atgtaagtag ggaccaaatt
    15421 gttcttctac tgctttcaaa gttgtttgta aatcatctgc tccctgctcg caaagggtat
    15481 gaaaatgaag tccgtcaacc aaatctagca aatcactcgg aatcttgtct atagtaactc
    15541 caaagcgaga acctggtgca caagggtcat agagcgcgtg atctccttga gttgaacact
    15601 gagggttgag gcgcaaacca acactgacac cagcctctcg acaacgagcc ccatgcttac
    15661 gcaactgtct ctctgagtta aagacgatat ggtctgttat cccaagcaat tcctccaagt
    15721 ctgcatcctt gaaagcaggc gcaaatacat ggacttcacc aggaaattct tcccttgcca
    15781 atttggcctc atagagtcca ctagctgtcg tacctgatag atactggcta atcaagggat
    15841 aagttttgta gagggaatat gccttctggg caagcaagac cttgcaaccg gcctcttctt
    15901 gtacatattg tagaatgcgg caattagctt ctaacttggc caagtcaata acataggctg
    15961 gtgttggtac ttgttctaac ttcattagtc caccatttgt ggattttcaa ccacaaccca
    16021 tggcaaacca tactcattca aagcttccat gaatggatct ggatctaact cctcaaggtt
    16081 atacactcca gcttgtttcc aagttccgtt catgactaat tttgtcccaa tcatggctgg
    16141 aactcctgtc gtgtatgaaa ttgcttgtga cccgacttct gcatagcact cttgatggtc
    16201 gcaaacattg tagatgtaga tagtcttttc aacgccatct ttgacacctg taaagataca
    16261 tccaatattg gttttaccta cagtacgtgg gccaaggcta gcaggatctg gaagcaaggc
    16321 tttcaaaaat tgaattggaa caatttcttg gccgttaaag ttaatggtat ccgtacgaag
    16381 gagtccaaca ttttcaagac atttcatgtg cgtcaagtaa gattgaccaa aagtcataaa
    16441 gaaacgaatg cgtttgacac ctggaatgtt cttggccaat gattcgattt cttcatggtg
    16501 aaggagatac atatcttttt gtccaacttg agggaaatca tactcacgct tgatagacat
    16561 agcttcgact tcgacccatt tcccatcttc ccagtaagaa cctggcgcag aaacctcacg
    16621 gagattaatt tctggattaa agttggttgc aaatggataa ccgtggtcac cgccattaca
    16681 gtctaaaatg tcgatataat ggatttcatc aaaatagtgt ttgagggcat aagctgaaaa
    16741 gacactagtt acacctgggt caaaaccaga accaagaaga gcagtcaagc ctgcttcttt
    16801 gaatttctct tgataagccc actgccatga gtagtcaaag taggctgtaa aaccaagttc
    16861 cttacaacgt ttttcgtaga tagcacgcca ctcagggtct tctgtgtctt ctgcttcgta
    16921 gttggctgta tcgatatagt gaacacctgt tgccaaacaa gcatccataa tggttaaatc
    16981 ttgataaggc agagctacat tcaaaacagc ttctggtttg tagctttcaa tcagggcaat
    17041 cacttcttca accttgtcag catcaagtgc tgcagtttca attttagtac ttgttttgcc
    17101 ttctagcttc gctttcaagt catcgcattt tgacttggta cggctagcaa tcataatctc
    17161 tgtaaatgtt tcgctatctt gacaaatctt tgaaatagca acttgggcaa cgcccccaca
    17221 accaataact agtaaacgac tcattttttt ccttcctctt cttctaaaat gtcctcaaca
    17281 tacttgggca acataaagga tcccacgtgt aagtttgcag tgtagtattc tgtgaaaagc
    17341 tggcgttttt tccaaccttc cttgtcaaaa tctttgacag ggtggtattt tttcgatgca
    17401 aatccaaaca accaatagcc agctgggcta gttggaatat gggcctgata aacccgactg
    17461 attggaaagg cttgattgac cttgcggtgc atgcttcggc aggccgactc atcctcgtca
    17521 aagaagggac tcccatgctg gtaaatcatg atgccgtctt ccttcagagc tcgataacta
    17581 ttgccgtaga attccttggt aaagagtcct tccgtatggc caaatggatc tgtcgcatcg
    17641 ttgatgataa tatcgtaatc atcttcgcag tttcgcaaaa agcgtagccc attttggtag
    17701 taaatggtaa cacgaggatc atctagccct gcagcaaagt ctgggaaata ctcacgacag
    17761 acctcgacca acatctcatc cggttccacg atatcgattt gttccagttc aggatagagt
    17821 gttaatactt gggcaacacc gccgtcacca cccccaataa ccaatacttt ctttggattt
    17881 gggtggacag ccatgggaac gtggacggtc atttcattgt agacgaaatc atccgcatct
    17941 gagaacaaga catggccatt taaaatcagt attttcccaa aagctggcgt atccaagact
    18001 tcgatatcct gccattcact ttttccagcg taaagttgct tggctgttct cagagacaat
    18061 ttgacatctg gagtatgaac ttcagaaaac cataaatcca tctatttctc cttatattat
    18121 atttagcgca gttagctact gccaaagccc aagtggtata cttggaataa gtcactgtgg
    18181 attagttcat ttcctccaat cgcttgacga tttccgaaag gctctaacca cagctttttg
    18241 tttcaattgg taatgagttt gataacgcat agacgtttta tctcgtctaa ggtaacaagg
    18301 caaaaagttc gtggataacc aagtagactt gaaatactat atctggaggt actcccatgc
    18361 tttatgtagg tattgatgtc gctaaaaata aacacgatgt tacagccttg aatgttcgag
    18421 gaaaactgtt cttaaaccac tcactttttc aaataataaa gctggttttg aactcttaga
    18481 tctgtctttt cgacagctca accaagactg tctcatcgct cttgaagata ctggacatta
    18541 tgcctttaat ctactgaact tcctgcatga acaaggatat aaagtttaca cctacactcc
    18601 tttgctcatc aaggaatttg ccaaatcatt atcacttcgc aagactaaaa cgaataagaa
    18661 agacgctcat ggcatcgctc ttaaacttct ttctgacccc aatcgtgaac aatttcaaca
    18721 cgataatcgg caagtagaac taaaaatact gactagacat attcatcgtc tcaagaaaaa
    18781 acagtctgat tggaaagtac aatacactcg ttgtcttgat atcatctttc ctgagttgga
    18841 taaaatcgtt ggaaagcatt cagaatatac ctaccaactc ttgacacgct accctaatcc
    18901 tcagaaaagg attgaggcag gatttgataa gctgatagaa attaagcgat tgaccgcttc
    18961 taattcaagg gctttttgcc gtgctctaaa atcatcgcca aatcatcaaa ttgaaataag
    19021 aaatagttta gataaaacta ttgacttttc ttatagtttg tctttctctt aataacgttg
    19081 atatgattga cctctggatc ttccgtccct tggagggagc aaccacgttc cttggcgaat
    19141 tggatatagt cgacaatttc tcgtgtaatg cgttcaccag gagccaagat aggaatccct
    19201 ggaggatagc acatgacaaa ttctccacag acctgtccaa cagaatcatc caaagttaaa
    19261 ctttttcttg ctgaatagaa ggcttcttgc ggagacagca ctaactcggg ctgaatatat
    19321 tctcctgcta tcaaatcttt tccatctctt gaatagagtc tcttaatatc agccagagca
    19381 ccaaccaagc gctcgatgtc ttggatgcgg tcgccgatag aaatataggc caagatattg
    19441 ccgatatcac caaactcgat ctgaatgtcg tattcgtctc gcaagaggtc ataaacctcg
    19501 atacctgtta agccaatacc ctgagtgtaa actgacagct tagttacgtc aaaatcgcaa
    19561 accgaaacac cgtctattaa ttcttttgag taggcatagt agccaccgat agcattgatt
    19621 tcacggcggg catactcaga tagctcaatg actttctcaa acgactcttt accacgaagg
    19681 gccaagttgc gacgtgaaat atccaaacta gccatcaaca agtaagaggc agatgtagac
    19741 tgggtcaggt ttataatctg acgaacgtac tcgggattca tctgctcccc gattaaaaga
    19801 agcgaacttt gtgtcaaact cccaccagac ttatgcatgg aaactgctgc catatcagct
    19861 cctgcatcca tagcagaaat tggaagttta ccagtgaaat gcaaatgcgc tccatgggct
    19921 tcatctacta aaaccatcat gccagcttca tgagccattt ctgtcaaccc ctttaggtct
    19981 gaacagatgc cgtagtaagt aggattgttg attaggatag ccttagcatc tggatggtcc
    20041 tttatggcct gtgctactcg gtcattttca agacctaaag cgataccaat cttaggatct
    20101 acactcatct cgatatagat gggaatggca ccacatagaa ccaacgcatt gatagcagat
    20161 ttatggacat ttcgtggcag aataatctta tctcctgcct tgcaggttgc cagaatcata
    20221 gtctgcaccg atgaagttgt tccaccaatc attagaaagg catggctagc tccaaaagca
    20281 tctgcagcca gctcctctgc atcacgaata atcgaaatag gatggcctaa attatccaag
    20341 ggtttcatcg aattgacatc aatgcctaca catttttctc ctaagagttc gacaagttct
    20401 ggatttcccc gtccacgctt gtgacctgga acatcaaagg gaacaatcct tttcttgcgt
    20461 aacttcacca aggcctcata aattggggct tggttttgat ctaactcttt caaaacatac
    20521 tcctttcaat atttttagga ccttatgaaa actaaggaac taaaggttga ctcatgaaaa
    20581 aagagcaggt tttcacctgc tctgcaatct tctgacgtgt ttattttggt ttctgttgag
    20641 tatgtctgtt cccgatgttt cctaactctc tagtagcaaa tattacgtac tttattgatc
    20701 gtttcacaag atgacgtgtg ctttcaccac actaaaaatt tatgaattag gacaagtgtc
    20761 ctaacatcaa cttataaaag taggctttta accctatcaa taatgtggct tttcaaccac
    20821 gcttcggcat tcaaccctgc ctgtccgtag gcatttttta ctcgggttta tatttgctag
    20881 aaaacatcat ctcattttct aagctttatt actatatcat gtttttaaga acttgtaaag
    20941 tattttttga taaaaaacaa aaatataata gattgaaact agaatagtac acctttgctt
    21001 ctaaaacatt gttagaaata gattttactg tcctgatcga tttgttctgt tcttgtttca
    21061 ttttactata tgttcgtaaa tgaaagttct ataatgaagt taaccaccca gccctcctaa
    21121 aaattagatg gttcaactag ctgatagctc ttgtaggcat aagaaaacac ctctgtgtta
    21181 tacttgttgt tcaaccacaa acacaagaaa ggcacaga
//
LOCUS       DAYYQN010000036        20685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYYQN010000036 DAYYQN010000000
VERSION     DAYYQN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.036"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            122..3223
                     /locus_tag="ACHBRQ_001311"
     CDS             122..3223
                     /locus_tag="ACHBRQ_001311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9592977.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRVAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAIIVPYFDRVESLELGCDYYIGVYPETLASEFHRPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALENLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGFVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYLIDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3306..4313
                     /gene="pfkA"
                     /locus_tag="ACHBRQ_001312"
     CDS             3306..4313
                     /gene="pfkA"
                     /locus_tag="ACHBRQ_001312"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9592978.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4372..5877
                     /gene="pyk"
                     /locus_tag="ACHBRQ_001313"
     CDS             4372..5877
                     /gene="pyk"
                     /locus_tag="ACHBRQ_001313"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9592979.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDEVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6621..6836)
                     /locus_tag="ACHBRQ_001314"
     CDS             complement(6621..6836)
                     /locus_tag="ACHBRQ_001314"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM9592980.1"
                     /translation="MEPKIRNSDYLFIKNTPQIDYNTIGIFQVDGTNYVKKLRQGYLK
                     KTQSRILRHPPRRKQPYSYHRKSCQAI"
     gene            7684..8556
                     /locus_tag="ACHBRQ_001315"
     CDS             7684..8556
                     /locus_tag="ACHBRQ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HFM9592981.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8702..9046)
                     /locus_tag="ACHBRQ_001316"
                     /pseudo
     CDS             complement(8702..9046)
                     /locus_tag="ACHBRQ_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9189..9500
                     /locus_tag="ACHBRQ_001317"
     CDS             9189..9500
                     /locus_tag="ACHBRQ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592982.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9504..10220
                     /locus_tag="ACHBRQ_001318"
     CDS             9504..10220
                     /locus_tag="ACHBRQ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9592983.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10350..11165)
                     /locus_tag="ACHBRQ_001319"
     CDS             complement(10350..11165)
                     /locus_tag="ACHBRQ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9592984.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11162..12067)
                     /locus_tag="ACHBRQ_001320"
     CDS             complement(11162..12067)
                     /locus_tag="ACHBRQ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9592985.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(12068..12199)
                     /locus_tag="ACHBRQ_001321"
     CDS             complement(12068..12199)
                     /locus_tag="ACHBRQ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9592986.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKAKAEKKN"
     gene            complement(12263..12412)
                     /locus_tag="ACHBRQ_001322"
     CDS             complement(12263..12412)
                     /locus_tag="ACHBRQ_001322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592987.1"
                     /translation="MKIKKQTRKLAAGCSKYCFEVVDKSHIYGKAKLTWFEEIFEVYQ
                     LSKTN"
     gene            12465..14594
                     /locus_tag="ACHBRQ_001323"
     CDS             12465..14594
                     /locus_tag="ACHBRQ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9592988.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14581..15030
                     /locus_tag="ACHBRQ_001324"
     CDS             14581..15030
                     /locus_tag="ACHBRQ_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9592989.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            15088..15360
                     /locus_tag="ACHBRQ_001325"
     CDS             15088..15360
                     /locus_tag="ACHBRQ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9592990.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15714..15905)
                     /locus_tag="ACHBRQ_001326"
     CDS             complement(15714..15905)
                     /locus_tag="ACHBRQ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9592991.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16333..17091
                     /locus_tag="ACHBRQ_001327"
     CDS             16333..17091
                     /locus_tag="ACHBRQ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9592992.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSATLLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQLFQEISDTLTVMASEVN"
     gene            17093..19081
                     /locus_tag="ACHBRQ_001328"
     CDS             17093..19081
                     /locus_tag="ACHBRQ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9592993.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19123..19797)
                     /locus_tag="ACHBRQ_001329"
     CDS             complement(19123..19797)
                     /locus_tag="ACHBRQ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HFM9592994.1"
                     /translation="MDSNFAGRLNILRAGVLDANNGIISIAGVVIGVASTTTNIWIIF
                     LSGFAAILAGAFSMAGREYVSVSTPKDTEEAAVLREKLLLDQDRELAKKSLYAAYIQN
                     GECKTSAQLLANKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTRGVTFLL
                     EQLFSI"
     gene            complement(19793..>20685)
                     /locus_tag="ACHBRQ_001330"
     CDS             complement(19793..>20685)
                     /locus_tag="ACHBRQ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9592995.1"
                     /translation="LLGKAPQTIHTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNR
                     KRSVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQD
                     LLYPRKGKALKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLV
                     LTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHI
                     YYAHPYAFWERGTNENHNRFIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPR
                     EDFWMANLNLKFSVRNKSRN"
BASE COUNT         6305 a         3784 c         4375 g         6221 t
ORIGIN      
        1 gatgacaaaa atacaaatga atagaaaata aattagtaag ctgatgaaat ttttctcaag
       61 agaagccatt tataggtgaa aatggtataa tatagtgaga aggatagagg agaagtgtaa
      121 attgatcgca caactagata caaaaacagt ctatagtttt atggaaagcg tcatttcgat
      181 cgaaaagtat gtgagagtag ctaaagaata cggctacact catttggcta tgatggatat
      241 tgacaatctt tatggcgctt tcgactttct agagattaca aaaaaatacg gcattcatcc
      301 tttgctaggg cttgaaatga cagtgtttgt agatgatcag ggagtgaatt tgcgcttttt
      361 agctctatct agtgtgggct atcagcagtt gatgaagctt tcgacagcca agatgcaggg
      421 ggagaaaact tggtcagtcc tgtcccagta cctggaggat atcgcgatca ttgtgcctta
      481 ttttgataga gttgagtcgt tagaactagg ctgtgattac tatatagggg tttatccaga
      541 aacactagca agcgaatttc atcgtcctat cttgcccctt tatcgggtca acgcttttga
      601 aagcagggat agagaagttc ttcaagtttt aacagcgatt aaagaaaatc taccgctcag
      661 agaagttccc ttgcgttcga gacaagatgt ctttatatca gcaagttctt tagagaaatt
      721 attccaagaa cgttttcctc aagccttgga aaacttagaa aagcttattt caggtatttc
      781 ttacgatttg gatactagtt tgaaactgcc tcgttttaat ccagctagac cagcagtaga
      841 ggagttgaga gagcgtgctg aactggggtt tgttcagaag gggttgacta gtaaagaata
      901 tcaagataga ctagaccaag aattgtctgt tattcatgat atgggctttg atgattattt
      961 cttggttgtt tgggatttgt tgcgttttgg acgatcgaat ggctattata tgggaatggg
     1021 aaggggttct gcagtaggca gtttggtttc ttatgcctta gacatcacgg ggattgaccc
     1081 agtagagaaa aatctgattt ttgaacgctt tcttaatcgt gaacgctata ccatgccaga
     1141 tattgatatt gatatcccag atatttatcg tccagatttt atcagatatg ttggtaataa
     1201 atatggtagt aaacatgcgg cacaaatcgt tactttttca acctttggag ccaagcaagc
     1261 tctacgagat gtcttgaaac gctttggtgt tcctgagtat gaattatctg caattactaa
     1321 gaaaatcagt tttcgtgaca atcttaagtc ggcctatgag ggaaatctcc agtttcgtca
     1381 gcaaatcaat agtaagttag aataccaaaa agcttttgag attgcttgca agatagaggg
     1441 ctatccaagg caaacctctg tccatgcggc tggtgttgta attagtgacc aagatttaac
     1501 caactacatt cctctaaagt atggtgatga aattccactg actcagtatg atgctcatgg
     1561 agttgaggct agcggacttt tgaagatgga ctttctggga ctacgaaatt tgacctttgt
     1621 tcagaagatg caagagttgc ttgctgaaac agaaggtatt catctgaaaa ttgaagaaat
     1681 cgatttagaa gacaaagaaa cgttagcttt atttgcctct ggtaatacaa aaggtatctt
     1741 tcaatttgag caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag tctgttttga
     1801 agatgtcgtc gcgactactt ctctaaatcg accgggtgct agtgactata tcaataattt
     1861 tgtggcaaga aagcatgggc aggaagaagt gactgttctg gatccagtac tggaggatat
     1921 tttggctcca acctacggca taatgctcta tcaggagcag gttatgcagg ttgcccagcg
     1981 acttgccgga tttagtcttg ggaaagccga tattttgcgt cgggctatgg ggaaaaagga
     2041 tgcctctgcc atgcatgaga tgagggcttc ctttattcaa ggttcattag aagctggtca
     2101 tactgtggaa aaagcagagc aggtctttga tgttatggag aagtttgcag gctatggttt
     2161 taacaggtca cacgcctatg cctactcagc attggccttc cagttggctt attttaaaac
     2221 acattatcca gccatatttt atcagatcat gttgaattct gccaacagtg attacttaat
     2281 agatgcactt gaagcaggtt ttgaagtggc gcctctgtcc atcaacacga ttccctatca
     2341 cgataaaatt gccaacaagg ccatctatct aggtttgaaa tccattaaag gagtcagtaa
     2401 tgatttagct ctctggatta ttgaacatag accttattct aacattgaag attttatagc
     2461 taaattacct gagaattatc tgaaacttcc tctgctagaa cctttggtaa aagttggtct
     2521 tttcgattca tttgaaaaaa atcgtcaaaa agtatttaat aacttagcta atctatttga
     2581 atttgtgaaa gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga
     2641 agattggacg gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag
     2701 caaacatcca ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caatcggaaa
     2761 tttgtcagaa aatagctatg ctattatttt ggttgaagtt cagaaaataa aagtgattcg
     2821 taccaaaaag ggtgaaaata tggccttctt acaggcagat gatagtaaga aaaaattgga
     2881 tgtcactctc ttttcagact tatatcgtca ggttggacag gaaataaaag agggagcctt
     2941 ctactatgta aaaggaaaaa tacaatcacg tgatggccgt ctgcaaatga ttgcacaaga
     3001 aataagagaa gcagttgctg aacgcttttg gatacaggtg aaaaatcatg aatcggatca
     3061 agaaatttca cgcattttag aacaatttaa aggcccaatc ccagtcatca tccggtatga
     3121 agaggaacag aaaaccatcg tttctcccca tcattttgta gctaaatcca atgaattaga
     3181 ggagaaattg aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa
     3241 gaattttaaa atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataaa
     3301 ccaatatgaa acgtattgct gttttgacta gtggcggaga cgcacctggt atgaacgctg
     3361 ccatccgtgc agttgttcgt caagcaattt cagaaggaat ggaagtgttt ggtatctatg
     3421 acggatatgc tggtatggtt gccggtgaaa ttcatcccct agatgcagct tcagtagggg
     3481 acatcatttc tcgtggtggt acttttcttc actcagctcg ttatccagag ttcgctcaac
     3541 ttgaagggca acttaaaggg attgagcaat tgaaaaaaca cggaattgaa ggtgtagttg
     3601 ttatcggtgg tgacggatct taccacggcg ctatgcgttt gactgaacat ggcttcccag
     3661 ctattggtct tccaggtaca atcgataacg atatcgttgg tactgacttt acaatcggtt
     3721 ttgacacagc ggttactact gccatggacg ctatcgataa gattcgtgat acatcatcaa
     3781 gtcaccgtcg tacttttgta attgaagtta tgggacgtaa cgctggtgat atcgctcttt
     3841 gggctggtat tgcaactggt gctgatgaaa tcatcatccc tgaagcaggc ttcaagatgg
     3901 aagatatcgt agcaagcatc aaagctggtt atgaatgtgg taaaaaacac aatatcatcg
     3961 tcttagctga aggtgtgatg tcagcggctg aatttggtca aaaacttaaa gaagctggag
     4021 atacaagcga ccttcgtgta acagaacttg gacatattca acgtggtggt tctccaactg
     4081 cgcgtgaccg tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt cttaaagaag
     4141 gtatcggtgg tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat ccaattcttg
     4201 gtactgcaga agaaggggca ttgtttagcc ttactgcaga aggtaagatt gtggttaaca
     4261 acccgcacaa agccgatatt gagctatcta gcttgaataa gagcttgtca taatttacaa
     4321 tttagttaat aagaccaaaa aggtcatata tataaaggag tcacaaaaat catgaacaaa
     4381 cgtgtaaaga tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc
     4441 ggtgaggacg gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct
     4501 aaattgattg aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa
     4561 gaacaaggtg agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt
     4621 ggtttccttc ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct
     4681 aaagaatatt catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa
     4741 tcaactcgtg aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgaagtt
     4801 gaagttggtc gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa
     4861 gatgatgcaa ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa
     4921 aaaggtgtga acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac
     4981 gacgatatcc gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt
     5041 actgcaaaag atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt
     5101 caattgttcg ctaaaatcga aaaccaacaa ggtatcgata acttagatga aatcatcgaa
     5161 gcagctgatg gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg
     5221 gttccagttt atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc
     5281 actgcaacaa acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta
     5341 tcagacgtat tcaacgctgt tatcgacgga actgacgcta caatgttgtc aggcgagtct
     5401 gcaaacggta aatacccact cgagtcagta actacaatgg ctacaatcga caagaacgct
     5461 caagctcttc ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag
     5521 acagaagtaa tggcttctgc tgttaaagat gctactagct caatggatat caaattggtt
     5581 gtaactctta ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct
     5641 gacatcttag cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt
     5701 gttatcccaa tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa
     5761 cgtaaagcgg tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt
     5821 gtgccagtag gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa
     5881 aaatataaaa acctatcata tccagctttc tagcctgtat gatgggctct ttttttgtcc
     5941 agggggcaaa aagggggcaa aactttcata atggattgat actcttcgaa aatctcttca
     6001 aaccacgtca gcgtcgcctt accgtatata tgttactgac ttcgtcagtt ctatctacaa
     6061 cctcaaagca gtgctttgag caacctgcgg ctagcttcct agtttgctct ttgattttca
     6121 ttgagtatga aataagatat gcataaattg attagaaagt caaatgaatt tctacaaatg
     6181 ttttagcaat cgcaatctac ttgtctagat tcgatctgat atattttcga tttaatgata
     6241 tggtatttaa aacctccaaa gtagcttgct ccattctttt acctacgtga gtctagatgt
     6301 tatttactgt tttagcgttt ttgtgttcta ctctaaccat tatagcattc ttctcagcta
     6361 gtgtactaag gagtgtgtgc ctgaaaatat gggaactaag gggctggttt atcggtttct
     6421 ctagtttagt atttgccttt tgcaaagtga tcttaaatgc ctttctctaa atttacatat
     6481 cactattgtt taacaaaatc taatctattt taggtcactt attctttttt tgaaatgtag
     6541 aatgaacttt ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat atcgccattc
     6601 tccccctttt ttaattctcc ctatatagcc tgacaacttt tccgatggta cgaatatggt
     6661 tgctttcgtc taggtggatg tcggagtatt cgggattgag tttttttgag gtagccttgg
     6721 cggagtttct tgacatagtt agtgccgtct acttggaaga tgccgatggt attatagtca
     6781 atctgtgggg tattcttgat aaataggtag tcgctgtttc ttatctttgg ctccatggac
     6841 ttgctgacga cataagcgat tgggtcgtag tcgtctggga taatggaaac tccataacta
     6901 aatcgtcgtc ctgcatcaag cggctacctg cagagataaa ctacctaaca cgagagtaag
     6961 tagtctgtct gtagtcgtcc agtctgatga tttttacgat acttcgtttt tctgatcata
     7021 cagttgcctc tcggcatagg tcagaacttt accttgtctg ggtggtgccc gttggtcgta
     7081 gatagattgg atatcgctag gagaatcctt ttgaactgga ggaaagaggg catcaatcaa
     7141 gctactgaat actttaacta agtcaaatat agtatttttc ttagtagacc taaccctttt
     7201 ttcataattt ctaatggtgt ttttacttat acctatctta gtacccaatt cttattgagt
     7261 ccaaccatta ctagtctata ttgttttata gttgattgag tttggaatag tacgctgtag
     7321 ctgctaaaac atttctagga attaatttgc ctttcctaat agagttgttc atatcttatt
     7381 tcaatctact atatttttca cctctaacaa tcgcaatctc ttctttatcc atgaacgaaa
     7441 acgctttcta tctttgtaag taaagcatca cacgaaatcc acgaaaatga aaacctttgt
     7501 tgtgttttcg taaaaaattt gttgacagag cacgaaacgc ctactataat taaatcaagt
     7561 ttaaggaaat aacaaaaaaa caaatcggag ggaaagacga tgaacacatt aaatgagaaa
     7621 gtaatcaata tctgtaaagc agtagttaaa gaaactttaa tccaagacat ttaggagaaa
     7681 gaaatgaaga aatcaagaaa attagccaca ttaggaatct gttcagcctt gtttttaggc
     7741 ttggctgctt gtcaacaaca acatgctact tctgagggga cgaatcaaag gcaaagcagt
     7801 tcagcgaaag ttccatggaa agcttcatac accaacctaa acaaccaggt aagtacagaa
     7861 gaggtcaaat ctctcttatc agctcacttg gatccaaata gtgttgatgc attttttaat
     7921 ctcgttaatg actataatac cattgtcggc tcaactggct tatcaggaga tttcacttcc
     7981 tttactcaca ccgaatacga tgttgagaaa atcagtcatc tctggaatca aaagaagggc
     8041 gatttcgttg ggaccaactg ccgtatcaat agttattgtc ttttgaaaaa ttcagtcacc
     8101 attccaaagc ttgaaaagaa tgaccagttg cttttcctag ataatgatgc gattgataaa
     8161 ggaaaggtct ttgattcaca agataaggaa gagtttgata ttctattttc gagagttcca
     8221 actgaggcaa ctacagatgt caaggttcac gctgaaaaga tggaaacatt cttctcacaa
     8281 tttcaattca atgaaaaagc tcgaatgctg tctgtagtct tgcacgacaa tctggatggc
     8341 gagtatctgt ttgtaggcca cgttggggtc ttagtacctg ctgatgacgg tttcttattt
     8401 gtagagaaat tgactttcga agagccctac caagcgatta aatttgctag taaggaagat
     8461 tgctacaagt atttgggcac caagtatgcg gattatacag gcgagggact ggctaagcct
     8521 tttatcatgg ataatgataa gtgggttaaa ctttaatagt gttatgatat gacagtaatc
     8581 ttaggatttt tatttctatt tttgatgact tggtgggtta tttcaatcgt taatagccag
     8641 aaaccacgca aaaatgaaac atttattggc tatatccaaa gatacgatat tgatgggaat
     8701 ggctatcaat cattaccgtg tcctcagaat tgagaggagt tcttgaaatc gtaaaactac
     8761 tttgaacaag agttacttca acccattggc tccgacggat taagttgctt tcgtgaacac
     8821 caaaatcagc cgcaatttct tcataagtgc ggtattctcg cacatattga agagtggcca
     8881 taagaaggtc ttctaggctt aatttaggtt ttcgtccccc ttttgtgtgt ttaagttgat
     8941 aagcagtttt taatacagct aacatctctt caaaagtggt gcgctgaaca ccaacaagac
     9001 atttaaatcg tgcatcagtt agttgcttac ttgcttcata attcatagaa ctactatacc
     9061 atattttgtt tcgcaggaag tctaatgcta aaatcttatc agtgagaaga tttcttaaag
     9121 tttattcttt gtagtttcat ctgtattagt atataattta acttagagaa aataggagga
     9181 ctaatcgaat ggaattaaaa gattttacag aaaaagaaca ggaaatgatt aagaaaaggc
     9241 ttacaatgtc taatattagt gacaaagaaa ctactgagaa gattctggcg ctagtaccac
     9301 aagacttgat taagcgaatc ccgttttttg tcagaaaaca tgctacaaca cgtacgatta
     9361 aacgcatttc aattgaatac cctgaactct acgctgtagc tcaaacaagt ggtgagattc
     9421 cagaaaaaga acgcgaagaa ttgcgtcaga ttatcacgac tatctttgaa caaaagatga
     9481 ataagcatag tattaagtag agaatgaaaa aatattttat tagcggtttg ggaagcaatg
     9541 cctatcatag caaggatttt tttcaagaac tagattcgca ggtctatttt ctaaatccat
     9601 atgaaaagca tcttcgagat gaaacagaat tgaaatcatg gtttaaaaat gagattgtag
     9661 aggaagaatc tatctgtctg ataggccatt ctcttggagg agatttagct cgttatttcg
     9721 catcggaatt tgaagaagta aagaaactga ttcttttgga tggtggctat ctagatttag
     9781 ataagatttt acctttggat acagagttag aggaaactaa aaattatatc aaatctcaaa
     9841 ttgtttcgga cttagctctt cttatttcta aagagaagtc cgaagcaaag cattggtcag
     9901 aaaatatgga gaaagctgta agacagtcct atcactggaa tgttgagtat aatagatatg
     9961 agttggctat aaattatgaa aatatagaag cgatactccg cctacggaga aaaatacaag
    10021 cttttaagag agaagtggga gataccttgt ttattagtcc tcgctatcct aatggagcta
    10081 catggagaga ggaagcccta aaagaattgc cagactattt taatactatt tttttagaaa
    10141 actttggcca tgagctttat actcaagcac ctaaagaaat cgctagtcta atgaatgagt
    10201 ggctcgctta ttttctatga gctacttgta ttgctggaaa tggtataaga tgaaaatcta
    10261 caagtcacta attttcatat aacaaaaaaa cgaacaggct ctttttctgt tattcagaag
    10321 agaacttgtt cgtttttatc ttgattttct taaaaagctc tatatacata gggattttct
    10381 ggtttatcga ctttggtaaa gctgtcgatt tccaaggttg gaaggttttg tttgagttct
    10441 ttatggtagt gattgaccag ttttcctttg gctgtaatcc aggtgttgtt ttcatactgc
    10501 cgggtattgc ccttggtcag caatccatag acaccagaat ctgcgataca gtggataatg
    10561 ccgaatcgga acagaaattg actattggca tgactggggt cgttatagac aaagcctgta
    10621 aactggattg tcttgccctc aaattcatct ggatagtcgt agatagcctc catgacttcc
    10681 atatagtttt cattagtgat ctgaatacta tcttgggata agtatttatc cgccgccgtt
    10741 cgcatttcct tttcataggc tgattttgaa aaataagaac tagtatctgg tttcaaatat
    10801 tggcttgtcg tcccttcact tgtctgaata gctagatccg ttccttccga tagggggaaa
    10861 tgataacctt tagcagaaac ggtctgagaa tccaagctaa cagttgggaa agttaagccg
    10921 atgacaagtg gaatagccag aagagaaata ctcgtcatct tggccaatcg gctgttcaga
    10981 tgactgtggg ttttgacttg cttcatccag atatacaatt gaacaattgc caagataaag
    11041 gaaagcacca tggagatata ggccagatag gaatagtgca tgttgatgta ctggtttagt
    11101 ttgcccgaca gatggaggta aatagtcagt tcaaaatagc cagctaaaac taaaaatcga
    11161 atcataggat gactccaatc aagagagaat aggctaaaac gaccagagtc acaatcgtaa
    11221 tgaagtgact aataaatcgt gctttcaagt aatttttcat catgagaata tttttgatat
    11281 ccagcattgg gccaatgacg agaaaggcca gaactggtgc caaaccgaaa ctcgagagaa
    11341 gagaagcacc tataaaggca tccgcctcac tacagagcga aagaagaaag gccaaaatca
    11401 tcaagagcag gatggcaaaa agaggagtcg cactgataga ggtcagaatc cgagtcggaa
    11461 cgtagacctg tattatagaa gcaaagaggc agccaaatac caaataacgc cccgtatcaa
    11521 aaaattcatc aatggcctgc acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag
    11581 aaaaatcatg ctcatgacaa gccagacgat tttctttctg aatcggttct tgccagaaaa
    11641 atcctagaaa aattcctagt attacagcca caagaatgga acccagagct cgtaataggg
    11701 cgacatggaa ggagttgcca aaggcagaat aggtcgcaaa aagaacaatg ggattgataa
    11761 caggtgctgt cacaagaaaa ggaacggctg tgtaactggg aacctttttt tccagaaaac
    11821 gattgatgat ggggacgatt ccacattcac aagaagggaa aagcataccg acaaaggtcc
    11881 caaaaaagat tctcccccaa cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga
    11941 tataaacttc aatcagccct gagacaatgc ttcctattag aacgaaggga agggcttcaa
    12001 tgataatgga gagaaaaatg gctccagttt gaagtacact agatgggagt tgttgaaaag
    12061 cagtcatcta gtttttcttt tctgctttgg cttttttctc tttgtcatca gggaaagtaa
    12121 cttgtttcaa atcagggagt ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt
    12181 ttgtaaattt tacagccata ggtgtacctc gattctaaag tctacctcta ttatactatt
    12241 attttgcgga aaagggagtt ttttaatttg ttttcgatag ttgatacact tcgaaaatct
    12301 cttcaaacca cgtcagcttc gccttaccgt atatatgtga cttatccaca acctcaaaac
    12361 agtattttga gcagcctgcg gctagcttcc tagtttgctt tttgattttc attgagtatg
    12421 aaaaggattg gagaaagcct tccagcttgt ggtataatag atttatggat aaaaaatatg
    12481 aaaaaatctc tcaggatttg ggagtgatgt taaagcaaat tgataccgtt ctaagtttga
    12541 cagctgaagg ggcgactatt ccctttatcg cgcgttatcg caaggacatg actggtagtc
    12601 tggatgaggt ggcgattaag gctattattg atttggataa aagtctgaca aatctcaatg
    12661 accgtaagga agctgtctta gctaagattc aagaacaagg taagttgacc aaggaattgg
    12721 aagaagctat cttagttgcc gaaaaattag cagacgttga agaactctat cttccttata
    12781 aggaaaagcg tcgtaccaag gcaaccattg cccgtgaagc tggactcttt cctcttgctc
    12841 gtttgatttt gcagaatata gttgacttag agaaagaagc tgaaaagttc gtctgtgaag
    12901 gatttgcgac tggcaaggaa gccttgaccg gtgcagttga tattttggtc gaagccttat
    12961 cggaagatgt gaccttgcgt tctatgactt atcaggaagt gctgagacac tctaaactta
    13021 cttctcaagc caaggatgaa agtcttgatg aaaagcaggt ttttcagatt tattatgatt
    13081 tttcagagac agttggaact atgcaaggct atcgtacctt ggctctcaat cgtggggaga
    13141 aacttggtgt cttgaagatc ggttttgaac atgcgacgga ccgtattctt gccttctttg
    13201 ctactcgttt caaggtgaaa aatgcttata ttgatgaagt tgttcagcaa tccgttaaga
    13261 aaaaggtctt gcctgctatt gagcgtcgta ttcggacaga attaactgag aaagctgaag
    13321 agggagctat ccaacttttt tctgacaatc tgcgcaatct cctcttggtt gctccattga
    13381 aagggcgcgt ggttcttggg tttgacccag cctttcgtac aggtgccaag ctagctgtcg
    13441 tggatgcaac aggaaaaatg ctgacaactc aggtcatcta tcctgttaaa ccagcatcag
    13501 ctcgtcaaat cgaagaagcc aagaaagatt tagcagattt aattggtcaa tacggtgtag
    13561 agattattgc cattggaaat ggaacggcca gtcgtgaaag tgaagctttt gtagcggaag
    13621 ttctgaaaga tttccctgaa gtcagctatg ttatcgttaa tgaaagtggt gcttctgtct
    13681 attctgccag cgaacttgct cgtcaggagt ttccagactt gaccgttgaa aaacgctctg
    13741 ccatttctat cgcccgtcgt ttgcaagatc ctcttgcgga attggtcaaa atcgatccta
    13801 agtcaattgg tgtcggtcaa taccaacacg atgtcagtca gaagaaacta tctgagagtc
    13861 tggactttgt tgtcgataca gtggttaacc aagttggtgt caatgtcaat acagctagcc
    13921 cagctcttct ttcacacgta gctggactca ataaaaccat ttctgaaaat atcgtcaaat
    13981 accgtgagga agagggaaaa atcacttcac gctcccaaat caagaaagtt cctcgtttgg
    14041 gggccaaggc ttttgaacag gctgctggtt tcctccgtat ccctgaaagt agcaatatcc
    14101 ttgataatac aggagttcac ccagaaaatt acgctgccgt taaggaactc ttcaaatgct
    14161 tggacattaa ggatctgaat gaagaggccc aaagtaaact caagtcgctt tcagtcaagg
    14221 aaatggcgca agagttagac cttggtccag aaactcttaa agatatcatt gcagatcttc
    14281 tcaaaccagg tcgagatttc cgtgattcct ttgatgctcc agttctccgt caagatgtcc
    14341 tagatatcaa ggacttagtg gttggacaaa agctagaagg agtggtgcgt aatgttgttg
    14401 atttcggtgc ttttgttgat atcggaattc atgaggacgg tctcatccat atttcccata
    14461 tgagtcgcaa gtttatcaaa caccctagtc aagtggtggc agttggagat ttggtgagcg
    14521 tttgggttaa gaaaattgat actgaacgtg aaaaagttaa tctgtctctc ctagctccaa
    14581 atgaaactga ctgattacgt caagcaggtt tcactagaag acttcggcag accttttatc
    14641 catcatgtcc agtggaatag gcgtctacgt tcgacaggtg ggcgattttt ccccaaagat
    14701 gggcatttgg attttaatcc caaggtttat caggaactgg ggttggacgt ttttaggaaa
    14761 attgtccgac atgaactctg tcattatcac ctctattttc aagggaaagg ctatcaacac
    14821 aaggatcggg attttaagga actgttgaaa gcagtggatg gattacggtt tgtaccatcc
    14881 ttgcccaata gtaactccaa gccaatcaag ctctatcgtt gccaatcctg ccagcaaact
    14941 tatcagcgta agcgtaggat tgatacccaa cgctatcgct gtggactttg tcgaggaaag
    15001 ttgctattag taaatcagcc tgaggactga tgaaagccga gcctatccgt gatatactag
    15061 tctaacctag cagaaagagg aaatatcatg aacagtaagt tttataaaat gagacgaaat
    15121 cgcatgattt caggagtttt agcaggtctg tcagacaagt ggaactttga tgttaccctt
    15181 gtacgttttc tgtttgccat ttttacagtc gcaaactttg gacttggggt gattatctat
    15241 atcatcctag cttctatcat gccaaccaag gaagaaatcg aagcagaaat gtacggaaca
    15301 ggcccacgta aacgcaaaga agctcaagcc attgacgata atgaaggctg gttttggtga
    15361 ggttttacaa aagatttgga atacgcaaaa agctttaaat caatagtcta aactgcttga
    15421 tttaaagctt ttttactttt atactcaata aaaatcaaag tgcaaactag gaagccagcc
    15481 gcaggctgta atttagtcgg caatgtgaag ttgatatagt ttgcactttg atttttgaat
    15541 agtattaata atatttcgcc agcttcatcc acaaactata gtggattgga actagaatag
    15601 gacacctata cttctaaaaa attgttagaa atcgatttga ctgtcctgat cgatttgtcc
    15661 tgttcttatt tcattttatt ataaaagcag taatttaaac gcagtttttt aatctatttt
    15721 tgctgttgag acaatacatt tttggaaaaa tggtggatgc tgataacaac tgataaccag
    15781 caaataacat aagcagtgcc ggtaaattct gtactgataa agtgtgtgtg ttcaactaag
    15841 agctgaccga tgaatggaga gagcagatag agaagtccga tgagtatatg ttctttgtgt
    15901 ttcattaaag cgcaccttct ttccttatag gttgaacgac ttgcaagtgt taaattgaat
    15961 ggtatgttat ctaggtaagc ttactaaaat gttagttccc gcttcctcct tttcaaagta
    16021 agccttttca cacttatatt ttacaacttt aaaaatagga aatcaaatca ttttctaagt
    16081 aattttgctt atatttcacc caatcttaca aaaatgtaag attaaagtct cttttgtaag
    16141 gttaggttat ggcaagcggt ctttttttga tgtaaactag actcataaag aacaaaggag
    16201 taacatcatg aaacaaatct tacataagaa aactagaaaa ccaagtcaca aagaaattga
    16261 gcgcgttcag ctgggatgca ctatgatgca agctacattt catttgatgg gatattaaga
    16321 aaggagattc tcatgacact tttagatgta aaacacgttc aaaaaattta taaaacacgt
    16381 tttcagggca accaagtaga agccctcaag gatattcact ttaccgtaga aaagggtgac
    16441 tacgttgcca tcatgggtga gtctggttct ggtaaatcaa ctcttctcaa tattctagct
    16501 atgttggata aaccaactcg tgggcaggtt tacttgaatg gaactgacac cgcaactatt
    16561 aaaaattcac aggcttctag tttccggcgt gaaaagctag gatttgtctt ccaagacttt
    16621 aacttgctag atactctgtc tgttaaggac aatatcttgc ttccgcttgt cttgtcaaga
    16681 agacctataa cggagatgat gaagaaattg gtggtgacag ctgagaatct gggtattaac
    16741 caattgcaag agaagtaccc ttacgagatt tctggtggcc agaaacagcg tgtagcagta
    16801 gcccgcgcca tcatcacaga acctgaaatt ctccttgcgg acgagccaac aggagccctt
    16861 gattccaagt catctgcaac cttacttgat gtctttaatg aaatcaatga gcgtgggcaa
    16921 accatcctca tggtaaccca ctcaacagca gctgctagca gggccaagcg tgttctcttt
    16981 atcaaagacg gcattcttta caaccaaatc taccgtggag agaagacaga gcgtcagttg
    17041 ttccaagaaa tctctgatac cttgactgtc atggcaagcg aggtgaatta gtatgtttcg
    17101 attaaccaat aagttagcgg tatcgaactt gattaaaaac cgcaaactct actatccctt
    17161 tgcactggct gttctcttgg cagtcaccat cacctatctc ttttactccc taaccttcaa
    17221 tcccaagatt gcggaaatcc gtggaggaac caccattcaa gcaacacttg gatttggtat
    17281 gtttgtcgtt acccttgcgt cagccattat cgtcctctat gccaatagtt ttgtcatgaa
    17341 aaaccgttcc aaggaactgg gtatatatgg catgttaggc ttggagaagc gccatctaat
    17401 cagtatgacc tttaaggagt tagtgctatt tgggattcta actgttggag caggaatcgg
    17461 tattggagcc ttgtttgaca agttgatttt cgctttccta ctcaaactga tgaaattgaa
    17521 ggtggaactg gtcgctacct tccagatgaa agttgtcatt acagtacttc ttgtcttcgg
    17581 tttgattttc ctaggcctca tgttcctgaa tgcccttcga atcgcccgta tgaatgccct
    17641 ccagctctca cgtgagaaag ctagcggaga gaaaaaaggt cgcttccttc ctctccaaac
    17701 tattcttggt tccataagtt tagggattgg atattatctt gcccttacgg taaaagatcc
    17761 tcttacagcc ttaacaacct tcttcatagc tgttttactg gttatctttg ggacttatct
    17821 tttgttcaat gcagggatta ccgttttcct tcaaatctta aagaaaaata agaaatacta
    17881 ttaccaacct aataatctca tatctgtttc taacttgatt ttccgtatga agaaaaatgc
    17941 agttggacta gcaactatcg ctattttgtc aacaatggtt ttggtaacca tgtcagcagc
    18001 gacaagcatt ttcaattccg cagaatcctt taaaaaagtt ctaaatcctc atgattttgg
    18061 ggtttcaggg caaaatgttg aaaaagaaga tttggacaaa ctcttgagcc agtttgcaag
    18121 tgacaaaggt tataagatta aagagaaaga agtgcttcgt tacagtaact ttggtattgc
    18181 aaatcaagaa ggaaccaagt taactatttt tgaaaaagga caaaaccgtg tccaaccaaa
    18241 aacagttttc atggtatttg accaaaaaga ttatgaaaat atgactggtc aaaaactgtc
    18301 tctatcagga aatgaggtcg gtctctttgc caaaaatgac ggactgaaag gacagaaagc
    18361 tctaactcta aatgatcatc aattttctgt caaagaagaa tttaataaag atttcattgt
    18421 caaccatgtt ccaaataagt ttaatatctt gactactgat tacaattacc ttgttgttcc
    18481 tgatttacaa gcctttttgg atcaattccc agattcggct atctataatc agttttacgg
    18541 tggtatgaat gtagatgcca gcgaagaaga acaactcaag gtcgctgagg agtatgaaaa
    18601 ctacctcaat caatttaatg ctcaattaga cacagaaggt agctatgttt atggtagcaa
    18661 tctagcagat gctagttctc agatgagtgc cctctttggt ggtgtcttct ttatcggtat
    18721 tttcctatcc attatcttta tggtcggaac tgttctggtc atctactaca aacaaatttc
    18781 tgaaggctac gaagaccgtg aacgctttat tatcttgcag aaagtcggtt tggaccaaaa
    18841 gcaaatcaag caaaccatca acaaacaggt tttaactgtt ttcttccttc ctttgctctt
    18901 tgccttcata catctcgcct ttgcctacca tatgcttagc ctgattttaa aagtgattgg
    18961 tgtactggat acgactatga tgttgattgt gaccttgtct atctgcgcta tcttcctcat
    19021 cgcctatgtg ctgattttta tgattacttc aagaagttat cgcaagattg tgcaaatgta
    19081 aaaaagatac ctcgacttca aaatcgaggt atttcttgta ttctaaatgc tgaaaagttg
    19141 ttcgagcagg aaggtaactc ccctggtcaa gagaccaata gcaaggttcc gaatcatagc
    19201 tgttctagtc ggatcctttc caagtctggc actagtgtaa ccagtgagaa gaagggccac
    19261 accgacaata aggacagtag cagggatgcg atattcactt gggaatatgg tcactgacag
    19321 cattggaggc aaacttctaa ggaaaaaggc aacgaagcta gaaatggcag cgtgccaagg
    19381 attggtaaat tcttcatact caatcccata tttttcctct accagagcct tgagtggatt
    19441 tttaagaaag atcttattag ccaagagttg ggcagaagtt ttgcattctc cattttggat
    19501 ataagcagca tagagggatt ttttggctag ttccctatct tggtctagca agagtttttc
    19561 tcgcaaaacg gcagcttcct cggtatcttt tggagttgaa acggatacat attctctacc
    19621 agccattgaa aaggcaccag ctaagatagc cgcaaaacct gataaaaaga taatccagat
    19681 attggtcgtg gtactggcaa ctccgataac cacaccagca atggaaataa ttccattgtt
    19741 agcatcaaga acacccgcac gcaggatatt taaacgacct gcaaaatttg aatcaatttc
    19801 gtgatttgtt tctgacgcta aatttcaagt tcaagttagc catccagaag tcttctctgg
    19861 gtgacttgta gtccaagcat tttttaggat agttgttaat ccacttttcg atgaatgcga
    19921 cttctttggg agtcattttc ttggttccct taggtaacca tctacgaatg aacctgttgt
    19981 gattctcatt agttcccctt tcccaaaagg cataggggtg cgcataataa atgtgctcct
    20041 cagaaaatac atcaaacaag cgattgaatt ccgttccatt atctgccgtg atggaaagaa
    20101 tcttgtgttg ttttaagatg agttttagag cctgattgac cacctcagca cttttatttg
    20161 gaatcaatcg gatgatctga tgtctactct ttcgatccgt caagacaagc aagcagtagt
    20221 ttttcgctct cgtaagtaga accgtatcaa tctcataatg cccattctcc aagcgaagat
    20281 tgatagcttc agaccgctgt tcgatggatt gaccagcagg tttaaagttg gtgctagcct
    20341 gtttcttaag cgcttttcct tttctagggt aaagcagatc ctgtttgctt aaccccaatt
    20401 ttccatgatg aatccaatag taaatggttg aaattcccac gttaacccct ttagccatca
    20461 ccatcatttc aggcgaaaat ttttggttat gatagtggag aatcttttcc tttagttcct
    20521 tggtcaagct tgatttcttg accgagcgct tgcgattgtt ttcataagac tgttgagcat
    20581 agtcggcaga ataaacctct ttgaagcgcc cttttccaag acattgtcgg actgtcccac
    20641 gcttgatttc agtgtggata gtttgaggag cttttccaag tagag
//
LOCUS       DAYYQN010000037        20635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYYQN010000037 DAYYQN010000000
VERSION     DAYYQN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.037"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..83
                     /locus_tag="ACHBRQ_001331"
     rRNA            <1..83
                     /locus_tag="ACHBRQ_001331"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            160..275
                     /gene="rrf"
                     /locus_tag="ACHBRQ_001332"
     rRNA            160..275
                     /gene="rrf"
                     /locus_tag="ACHBRQ_001332"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            280..352
                     /locus_tag="ACHBRQ_001333"
     tRNA            280..352
                     /locus_tag="ACHBRQ_001333"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:Val,seq:tac)
     gene            358..428
                     /locus_tag="ACHBRQ_001334"
     tRNA            358..428
                     /locus_tag="ACHBRQ_001334"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:390..392,aa:Gly,seq:tcc)
     gene            463..536
                     /locus_tag="ACHBRQ_001335"
     tRNA            463..536
                     /locus_tag="ACHBRQ_001335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:497..499,aa:Ile,seq:gat)
     gene            619..708
                     /locus_tag="ACHBRQ_001336"
     tRNA            619..708
                     /locus_tag="ACHBRQ_001336"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:655..657,aa:Ser,seq:tga)
     gene            719..792
                     /locus_tag="ACHBRQ_001337"
     tRNA            719..792
                     /locus_tag="ACHBRQ_001337"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:753..755,aa:Met,seq:cat)
     gene            796..868
                     /locus_tag="ACHBRQ_001338"
     tRNA            796..868
                     /locus_tag="ACHBRQ_001338"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:829..831,aa:Phe,seq:gaa)
     gene            881..961
                     /locus_tag="ACHBRQ_001339"
     tRNA            881..961
                     /locus_tag="ACHBRQ_001339"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:915..917,aa:Tyr,seq:gta)
     gene            966..1036
                     /locus_tag="ACHBRQ_001340"
     tRNA            966..1036
                     /locus_tag="ACHBRQ_001340"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:998..1000,aa:Trp,seq:cca)
     gene            1051..1123
                     /locus_tag="ACHBRQ_001341"
     tRNA            1051..1123
                     /locus_tag="ACHBRQ_001341"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1084..1086,aa:His,seq:gtg)
     gene            1137..1208
                     /locus_tag="ACHBRQ_001342"
     tRNA            1137..1208
                     /locus_tag="ACHBRQ_001342"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1169..1171,aa:Gln,seq:ttg)
     gene            1218..1301
                     /locus_tag="ACHBRQ_001343"
     tRNA            1218..1301
                     /locus_tag="ACHBRQ_001343"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1252..1254,aa:Leu,seq:caa)
     gene            1408..2685
                     /locus_tag="ACHBRQ_001344"
     CDS             1408..2685
                     /locus_tag="ACHBRQ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HFM9592996.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2761..4245
                     /gene="thrC"
                     /locus_tag="ACHBRQ_001345"
     CDS             2761..4245
                     /gene="thrC"
                     /locus_tag="ACHBRQ_001345"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFM9592997.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            4306..5586
                     /locus_tag="ACHBRQ_001346"
     CDS             4306..5586
                     /locus_tag="ACHBRQ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9592998.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5590..6210
                     /locus_tag="ACHBRQ_001347"
     CDS             5590..6210
                     /locus_tag="ACHBRQ_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9592999.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6376..7488
                     /locus_tag="ACHBRQ_001348"
     CDS             6376..7488
                     /locus_tag="ACHBRQ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9593000.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7811..8236
                     /locus_tag="ACHBRQ_001349"
     CDS             7811..8236
                     /locus_tag="ACHBRQ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9593001.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            8247..8606
                     /locus_tag="ACHBRQ_001350"
     CDS             8247..8606
                     /locus_tag="ACHBRQ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9593002.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATKSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8713..9357
                     /gene="pcp"
                     /locus_tag="ACHBRQ_001351"
     CDS             8713..9357
                     /gene="pcp"
                     /locus_tag="ACHBRQ_001351"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9593003.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9385..10242)
                     /locus_tag="ACHBRQ_001352"
     CDS             complement(9385..10242)
                     /locus_tag="ACHBRQ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9593004.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10372..11514
                     /gene="tgt"
                     /locus_tag="ACHBRQ_001353"
     CDS             10372..11514
                     /gene="tgt"
                     /locus_tag="ACHBRQ_001353"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9593005.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11612..13429
                     /locus_tag="ACHBRQ_001354"
     CDS             11612..13429
                     /locus_tag="ACHBRQ_001354"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9593006.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13582..14733
                     /gene="nagA"
                     /locus_tag="ACHBRQ_001355"
     CDS             13582..14733
                     /gene="nagA"
                     /locus_tag="ACHBRQ_001355"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9593007.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14896..15954
                     /locus_tag="ACHBRQ_001356"
     CDS             14896..15954
                     /locus_tag="ACHBRQ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9593008.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            16105..16470
                     /locus_tag="ACHBRQ_001357"
     CDS             16105..16470
                     /locus_tag="ACHBRQ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9593009.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16545..17486
                     /gene="comGA"
                     /locus_tag="ACHBRQ_001358"
     CDS             16545..17486
                     /gene="comGA"
                     /locus_tag="ACHBRQ_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9593010.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17434..18450
                     /gene="comGB"
                     /locus_tag="ACHBRQ_001359"
     CDS             17434..18450
                     /gene="comGB"
                     /locus_tag="ACHBRQ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9593011.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18452..18778
                     /gene="comGC"
                     /locus_tag="ACHBRQ_001360"
     CDS             18452..18778
                     /gene="comGC"
                     /locus_tag="ACHBRQ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9593012.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18771..19175
                     /gene="comGD"
                     /locus_tag="ACHBRQ_001361"
     CDS             18771..19175
                     /gene="comGD"
                     /locus_tag="ACHBRQ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9593013.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            19138..19440
                     /gene="comGE"
                     /locus_tag="ACHBRQ_001362"
     CDS             19138..19440
                     /gene="comGE"
                     /locus_tag="ACHBRQ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HFM9593014.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19403..19864
                     /gene="comGF"
                     /locus_tag="ACHBRQ_001363"
     CDS             19403..19864
                     /gene="comGF"
                     /locus_tag="ACHBRQ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9593015.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19842..20288
                     /gene="comGG"
                     /locus_tag="ACHBRQ_001364"
     CDS             19842..20288
                     /gene="comGG"
                     /locus_tag="ACHBRQ_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9593016.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            20391..>20635
                     /locus_tag="ACHBRQ_001365"
     CDS             20391..>20635
                     /locus_tag="ACHBRQ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9593017.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMGSIIFVVGKST"
BASE COUNT         6064 a         3694 c         4820 g         6057 t
ORIGIN      
        1 agatgatcag gtagataggt tagaagtgga agtgtggcga cacatgtagc ggactaatac
       61 taatagctcg aggacttatc caaagtaact gagaatatga aagcgaacgg ttttcttaag
      121 ttgaatagat attcaatttt gagtaggtat tactcagagt taagtgacga tagcctagga
      181 gatacacctg tacccatgcc gaacacagaa gttaagccct agaacgccgg aagtagttgg
      241 gggttgcccc ctgtgagata gggaagtcgc ttagctctag ggagtttagc tcagctggga
      301 gagcatctgc cttacaagca gagggtcagc ggttcgatcc cgttaactcc cattttagcg
      361 ggtgtagttt agtggtaaaa ctacagcctt ccaagctgtt gtcgcgagtt cgattctcgt
      421 cacccgcttt gaactttgtt ctttgtacca agtttttgac ttgggcgcgt agctcaggtg
      481 gttagagcgc acgcctgata agcgtgaggt cggtggttcg agtccactcg tgcccatagt
      541 gtttagtcca ttactagggg attggaatat tatctgttca ctaagaggac acgggcttgt
      601 tcccgtataa actattttgg aggattaccc aagtccggct gaagggaacg gtcttgaaaa
      661 ccgtcaggcg tgtaaaagcg tgcgtgggtt cgaatcccac atcctccttt tatattaacg
      721 cgggatggag cagctcggta gctcgtcggg ctcataaccc gaaggtcgta ggttcaaatc
      781 ctgctcccgc aataaggctc ggtagctcag ttggtagagc aatggattga agctccatgt
      841 gtcggcggtt cgattccgtc tcgcgccatt tatatatttt ggaagggtag cgaagaggct
      901 aaacgcggcg gactgtaaat ccgctccttc gggttcgggg gttcgaatcc ctccccttcc
      961 attttacggg catagtttaa aggtagaact aaggtctcca aaaccttcag tgtgggttca
     1021 attcctactg cccgtgttaa tagaattatg gcgggtgtgg tgaagtggtt aacacaccag
     1081 attgtggctc tggcatgcgt gggttcgatc cccatcactc gcctatttta tattattggg
     1141 gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt cgaatccagc
     1201 taccccagtt actatttgcc ggcgtggcgg aattggcaga cgcgctggac tcaaaatcca
     1261 gtgtccgcaa ggacgtgccg gttcgacccc ggccgccggt atagtatagt gttaggaacg
     1321 ttgttattct tcgttccttt tttatattat ttttggtata attatagtta ttcaaatttt
     1381 atttagatta agaaagtgta ggggagtatg tcttgttcta tcgatttatt aaaacatcgg
     1441 tatttgaaaa atattaaaga aaatcctgaa ttgtttgtcg gaattgagtt ggagtatcct
     1501 gttgcaagtt tagaagggga tgctacagat gttgaagtta tgaaggatct atttcattat
     1561 ttagtttcta ctttggatct caccgtagca aaggtagatg attttggcaa tctgatccag
     1621 ttagtagatc cgataagtca ggatgctatt ttatttgaag tttcctatac aacgattgag
     1681 tttgcatttg gtaaggctga aacgattcaa gaggtcgaaa atcgtttcaa taattatatg
     1741 aatgtaattc agagaaagtt agctgaatca aatcatgcta ttgttggctg tggtatccat
     1801 cccaactggg ataaaaatga gaattgtcca gtggcttatc cacgctatca gatgttgatg
     1861 gattatttga atttgagtag aaatattatt aaatcagatt tacatcattt ccctgaatat
     1921 ggtactttta tctgtgggag ccaggttcag ctggatattt caaaaaccaa ctacttacgg
     1981 gtgattaatg cttttactca aattgaagcg gctaaggctt atttatttgc aaactctgaa
     2041 ttttcgggtg cggattggga tacgaaaatt tcaagggata ttttctggga agaatctatg
     2101 catggtatct atccagagaa tgttggggtc aatgctagac tccttaatga tgaagctgat
     2161 ttttttgact atctaaatca ttctgcgatt tttactgcgg aacgtgatgg gcagacctat
     2221 tatttttatc ctattcaggc tggggactat ttggctacgt ccgaaatcca agcatttgct
     2281 ctgaatgggg atgaggttat tatttacccc caagagaagg attttgaaac tcatcgtagt
     2341 taccagtacc aagatttaac gactcgagga acagttgagt ttcgtagtgt gtgtacacag
     2401 ccacttgata ggacttttgc ttctgcagct tttcacttgg gattattggt taatttagac
     2461 aagttagaag cttacttaga aacagcacct ttctttaaag tatttggtta tgattacaag
     2521 tctttaagga gacaattttc taagaaaaat cttacagatg aggaagaaac tacgattatt
     2581 gaattttcca aagacttact cctactagct gaggagggac tagtggtgag aaataaggaa
     2641 gaaatgacct atttacagcc tttgagagaa gaattgagcc tataatttct cttataaagg
     2701 gagaattttc tgaaaaatca tgatataatg gacgagacta tagataaagg atagagagta
     2761 atgacattag tttatcaatc aacgcgtgat gccaacaata cagtaactgc cagccaagca
     2821 attttgcaag gtttggcgac ggacggcggt ttgtttacac cggatactta tccaaaggta
     2881 gatttgaact ttgacaaatt gaaagatgct tcttaccagg aagttgctaa gctagttttg
     2941 tcagcatttt tagatgactt tacagttgag gagttggact actgtatcaa caatgcctac
     3001 gatagcaaat ttgatactcc agctattgca ccattagtga aattagatgg gcaatacaat
     3061 ttggaacttt tccatggttc aacgattgcc tttaaggata tggccttgtc tattttgcca
     3121 tactttatga cgactgctgc taagaaacat ggtttggaga acaagattgt tatcttgaca
     3181 gcgacatctg gtgacacggg gaaagctgct atggcggggt ttgcgaatgt gcctggtact
     3241 gagattatcg tcttttatcc aaaggatggt gtcagcaaga ttcaagagtt acaaatgacc
     3301 actcagactg gcgacaatac tcatgttatt gctattgatg gtaactttga cgatgcgcaa
     3361 acaaatgtga agcacatgtt taacgacgtg gctcttcgtg aaaaattgac taccaacaag
     3421 ttgcaatttt catcagctaa ctctatgaac attggtcgtc tggtgccaca aattgtttat
     3481 tatgtttatg cttacgctca attggttaag actggtgaaa ttgtagctgg tgaaaaggtt
     3541 aacttcacag taccaacagg aaactttgga aatatcttgg ctgcctttta tgccaaacaa
     3601 attggtttgc cagttggtaa attaatctgt gcttcaaatg acaacaatgt tttgacagac
     3661 ttctttaaaa cacgtgtcta tgacaaaaaa cgtgagttta aggtaacaac cagcccatct
     3721 atggatatct tggtatcttc aaacttggag cgcttgattt tccatctttt gggaaataat
     3781 gctgaaaaga caactgaact tatgaatgcc ttgaacacgc aaggacaata taagttgaca
     3841 gactttgatg cagagatttt ggacctcttt gcagctgaat atgcgactga ggaagaaacg
     3901 gcagcagaga tcaagcgtgt ttgtgagtta gattcttata tcgaggaccc tcatacagct
     3961 gttgcttcag cagtttataa aaaataccaa tcggccactg gagatgtaac taagacagtg
     4021 attgcttcaa cagctagtcc atacaagttc ccagtagttg cagtagaagc tgtaactgga
     4081 aaagcaggtt taacagactt tgaagccttg gctcaattac atgaaatctc aggcgttgca
     4141 gtgccaccag cagttgatgg gcttgaaata gctccaattc gtcacaagac aacagtggca
     4201 gctgctgaca tgcaagcagc ggttgaggct tatttaggac tttaagacag agggagcaaa
     4261 ctcggttggg aaaccaactg agtttctttt catcaggagg agagattgtt taagaaaaat
     4321 aaagacattc ttaatattgc attgccagct atgggtgaaa actttttgca gatgctaatg
     4381 ggaatggtgg acagttattt ggttgctcat ttaggattga tagctatttc aggggtttca
     4441 gtagctggta atattatcac catttatcag gcgattttca tcgctctggg agctgctatt
     4501 tccagtgtta tttcaaaaag catagggcag aaagaccagt cgaagttggc ctatcatgtg
     4561 actgaggcgt tgaagattac cttactatta agtttccttt taggattttt gtccatcttc
     4621 gctgggaaag agatgatagg acttttgggg acggagaggg atgtagctga gagtggtgga
     4681 ctgtatctat ctttggtagg cggatcgatt gttctcttag gtttaatgac tagtctagga
     4741 gccttgattc gtgcaacgca taatccacgt ctgcctctct atgttagttt tttatccaat
     4801 gccttgaata ttcttttttc aagtctagct atttttgttc tggatatggg gatagctggt
     4861 gttgcttggg ggacaattgt gtctcgtttg gttggtcttg tgattttgtg gtcacaatta
     4921 aaactgcctt atgggaagcc aacttttggt ttagataagg aactgttgac cttggcttta
     4981 ccagcagctg gagagcgact tatgatgagg gctggagatg tagtgatcat tgccttggtc
     5041 gtttcttttg ggacggaggc agttgctggg aatgcaatcg gagaagtctt gacccagttt
     5101 aactatatgc ctgcctttgg cgtcgctacg gcaacggtca tgctgttggc ccgagcagtt
     5161 ggagaggatg attggaaaag agttgctagt ttgagtaaac aaaccttttg gctttctctg
     5221 ttcctcatgt tgcccctgtc ctttagtata tatgtcttgg gtgtaccatt aactcatctc
     5281 tatacgactg attctctagc ggtggaggct agtgttctag tgacactgtt ttcactactt
     5341 gggaccccta tgacgacagg aacagtcatc tatacggcag tctggcaggg attaggaaat
     5401 gcacgcctcc ctttttatgc gacaagtata ggaatgtggt gtatccgcat tgggacagga
     5461 tatctgatgg ggattgtgct tggttggggc ttgcctggta tttgggcagg gtctctcttg
     5521 gataatggtt ttcgctggtt atttctacgc tatcgttacc agcgctatat gagcttgaaa
     5581 ggataggaaa tgcaaaaaac agcttttatt tgggatttag acgggacttt attggactct
     5641 tacgaagcga ttttatcagg gattgaggag acttttgctc agttttctat tccttatgat
     5701 aaggagaagg tgagagagtt tatcttcaag tattcggtgc aagatttgct tgtgcgggtg
     5761 gcagaagata gaaatctgga tgttgaggtg ctaaatcagg tgcgtgccca gagtctggct
     5821 gagaagaatg ctcaggtagt tttgatgcca ggtgcgcgtg aggtgctagc ttgggcagac
     5881 gaatcaggaa ttcagcagtt tatatatact cataagggga acaacgcttt taccattctc
     5941 aaggacttgg gggtggaatc ctattttaca gagattttaa ccagtcagag tggctttgtg
     6001 cggaagccaa gtccagaagc ggctacctat ctgctagata agtatcagtt gaattctgat
     6061 aatacttatt atatagggga tcggactctg gatgtggaat ttgcccagaa tagtgggatt
     6121 caaagcatca actttttaga gtctacttat gaagggaatc acaggattca agcgttagca
     6181 gatatttccc gtatttttga gactaagtga taaaaagatt gtgtcagttt tgtgacagag
     6241 acctaacaaa ctatttcaag taacctagtt tgttacaagg aatagacagt tctgttaaat
     6301 aggcccgaga gggctttttt tctacatttt ttgtgttatg atagacaggt actcatttga
     6361 aaggaatttg aaagaatgaa gaaaagaatg ttattagcgt caacagtagc cttgtcattt
     6421 gccccagtat tggcaactca agcagaagaa gttctttgga ctgcacgtag tgttgagcaa
     6481 atccaaaacg atttgactaa aacggacaac aaaacaagtt ataccgtaca gtatggtgat
     6541 actttgagca ccattgcaga agccttgggt gtagatgtaa cagtgcttgc gaatctgaac
     6601 aaaatcacta atatggactt gattttccca gaaactgttt tgacaacgac tgtcaatgaa
     6661 gcagaagaag taacagaagt tgaaatccaa acacctcaag cagactctag tgaagaagtg
     6721 acaactgcga cagcagattt gaccactaat caagtgaccg ttgatgatca aactgttcag
     6781 gttgcagacc tttctcaacc aattgcagaa gttacaaaga cagtgattgc ttctgaagaa
     6841 gtggcaccat ctacgggcac ttctgtccca gaggagcaaa cgaccgaaac aactcgccca
     6901 gttgaagaag caactcctca ggaaacgact ccagctgaga agcaggaaac acaagcaagc
     6961 cctcaagctg catcagcagt ggaagtaact acaacaagtt cagaagcaaa agaagtagca
     7021 tcatcaaatg gagctacagc agcagtttct acttatcaac cagaagagac gaaaataatt
     7081 tcaacaactt acgaggctcc agctgcgccc gattatgctg gacttgcagt agcaaaatct
     7141 gaaaatgcag gtcttcaacc acaaacagct gcctttaaag aagaaattgc taacttgttt
     7201 ggcattacat cctttagtgg ttatcgtcca ggagacagtg gagatcacgg aaaaggtttg
     7261 gctatcgact ttatggtacc agaacgttca gaattagggg ataagattgc ggaatatgct
     7321 attcaaaata tggccagccg tggcattagt tacatcatct ggaaacaacg tttctatgct
     7381 ccattcgata gcaaatatgg gccagctaac acttggaacc caatgccaga ccgtggtagt
     7441 gtgacagaaa atcactatga tcacgttcac gtttcaatga atggataaac ccgacttgat
     7501 aacatcattt tgacgaatga gatctagctt tcgtgatgga aagcgattct cgttcgtttt
     7561 ttctttgtca tactcttcga aaatctcttc aaaccacgtc agttttatct gaaacttcaa
     7621 agctgtgctt tgagcaacct gcgactagct tcctagtttg ctttttgatt ttcattgagt
     7681 atcaatttga atggaaaatg gaaagttatc atcttgtaat gagttaagca acattcttgc
     7741 aatctatttt actttatatc acaattaatt agtcaaatat tgataaatca ataaaaagag
     7801 aggggaagaa atgctagaga ttcaagattt actgtatcaa ctccgcttgt ctgagcaagc
     7861 gagtacgcaa ttgtttgaaa aaaggcttgg gattagtttg acacggtatc agattttact
     7921 gtttttgctg gagcattctc cttgtaacca aatggcggtt caggagcgtt tgaaaattga
     7981 tcaggctgct ttgacacggc atttcaaaat tttggaaacg gaaggtttgg tggagcgtca
     8041 tcgtaatcct gaaaatcagc gggaagtgtt ggtagaggct gcgaagtatg ccaaggagca
     8101 gttagtggtg aatccccctc tgcaacatat cagggttaag gaagagatag aaagtatctt
     8161 aacagagttt gagagaacag aactcagccg tttattaaat aaattggttt tgggtattga
     8221 aaatatagaa atttaaggag aaatagatgt caattatttt aacaacgatc gttgctttgg
     8281 agcattttta cattttttat ttggaaagta ttgccacgaa atcagatgcg actagtcgtg
     8341 tatttaatat ggaaaaggaa gaattggctc atccgtcagt aagttcattg ttcaaaaatc
     8401 aaggaattta taaggctctg ctaggagtct ttctcttgta tgtcatttat ttctcacaga
     8461 atttagaaat tgtgactatt tttgtcttat ttgtgattgg tgctgcgact tacggctctt
     8521 taacagcgga taaaaaaatt attttgaaac aaggtggatc agctattttg gccttgatta
     8581 gtattttact ctttaaatac acttgaaggt cgattctaat ctcgctaatc ctttttaatc
     8641 cagaataagg gaaatatgtt atacttgttt ttaagaaaaa agtctcattg aattggtttt
     8701 gaggagttag aaatgaaagt attagtgaca ggttttgagc cctttggagg ggaaaagggc
     8761 aatccagctt tggaggccat taaaggttta ccagctgaaa tccatggtgc tgaggtccgt
     8821 tggctagagg tgccgacagt ttttcacaaa tctgctcaag tattggaaga agagatgaat
     8881 cgttatcaac ctgactttgt cctttgtatt gggcaagctg gtggaagaac tagtttgaca
     8941 cctgaacgag tgaccattaa tcaagacgat gcatgcattt ctgataacga agataatcaa
     9001 ccgattgacc gtcccattcg cccagatggt gcttcggcct actttagtag tttgccgatt
     9061 aaagcgatgg ttcaagctat aaaaaaagag ggcttaccgg cctctgtttc caatacggca
     9121 gggacttttg tctgcagcca tttgatgtat caggctctct atttggtaga aaagaaatct
     9181 ccatatgtta aggcaggttt tatgcatatt ccttatatga tggaacaggt ggtgaacaga
     9241 ccgactactc cagctatgag tttagtggat attcggcgag ggatagaagc agcaatcggc
     9301 gctataatag aacatggaga tcaggaactc aagttggtag gcggagaaac tcattgatag
     9361 aaaaaagctt gaggggaaaa accttcaagc ttttggacgt tttcgggcca atactgctcg
     9421 gtaaaacata attttagtgc attggatata aggtaggagt gaaaaactag caatgccaaa
     9481 ggtaatccaa ttgaggaagt accaaggaag aagctgtaaa tctaggacaa agtgctggaa
     9541 cttgtagccc ttcataaagg aacggctagt ttttaggatt cgtcttggtg ggacctgtcc
     9601 taggtctaga ctataacaga gaagaaattc cacctgtgaa taggcataat actgtggaat
     9661 atagaggata tttcctacaa tgatcaagat gagacttgca agaaagtaga gtccaaagac
     9721 catgaggaaa cgctcggttt caactgatga gagatctaga tttggaaact caggatgtag
     9781 ggtgacgaat tttttggcta aaaagctact ataaaagagg aggtaaatcc caagtaaatt
     9841 agggatactc cataaaaaga gatagaaacg tttgagaagt agggtcaaaa aggtttgaga
     9901 aaagcgctcc tcatcaaaga gagctaggct gttttttaca gatggctccg ttttagaatc
     9961 tttcatgagt gtcagtgttg catagacgga actggtcaaa agaatagtcc cgataaagga
    10021 gactagtaga ggaaagaggt aggtttgaag tatttggcca agtatgctga aaaatggctg
    10081 ttctaaaaca gtcccgtgga tccgagataa gggattaaga aaaccagata agatgaccag
    10141 catactggga aggatataga ggagaaagag acggggggtg tcagcctgaa aatgttttga
    10201 ctcctgacga attgttttta aatcaatttt tggatagttc attctcttat tataccatag
    10261 ttcttataca tagttcgtga cagttcctac tttttttgat aaaatcatac agtgtgtcct
    10321 tgggcacact gtatgaactg ggactgtctt tcccagcttc ggaggtaaaa aatgtcagat
    10381 tcaccaatca aatatcgttt gattaagaaa gaaaaacaca caggagctcg tctgggagaa
    10441 atcatcactc cccacggtac ctttccgaca cctatgttta tgccagttgg gacacaagcc
    10501 actgtcaaaa ctcagtcacc tgaagaattg aaggagatgg gttcgggaat tatcctatca
    10561 aacacctatc atctctggct tcgccctgga gatgaactca ttgcacgcgc tggtggtctc
    10621 cacaagttca tgaattggga ccagcctatc ttgacagata gtggtggttt tcaggtttat
    10681 tctttagcag atagccgtaa tatcacagaa gaaggagtaa cctttaaaaa tcatctaaat
    10741 ggttctaaga tgttcctatc cccagaaaaa gccatctcta ttcagaataa tctgggttca
    10801 gacatcatga tgtcctttga tgaatgtcct cagttttatc aaccttatga ctacgttaag
    10861 aaatcgatcg agcgtaccag ccgttgggct gagcgtggtt tgaaggctca ccgtcgtcca
    10921 catgaccaag gtttgtttgg aattgtgcaa ggtgcaggat ttgaagacct tcgccgccaa
    10981 tcagctcatg atcttgtcag catggatttc tcaggctact ctatcggtgg tttggcagtg
    11041 ggagaaaccc atgaagagat gaatgcggtc ttggacttta caactcaact gctacctgaa
    11101 aataaacctc gttatctgat gggtgtggga gcgccagata gcttgatcga tggggtcatt
    11161 cgtggggtgg atatgtttga ctgtgtctta ccgactcgaa ttgctcgtaa cgggacttgt
    11221 atgaccagtc aaggacgttt ggttgtgaaa aatgcccagt ttgctgagga ctttacgcca
    11281 ctggatcctg agtgtgactg ctacacatgt aataactata cacgcgctta ccttcgtcac
    11341 ctgctcaagg ctgatgaaac ctttggtatc cgcttgacta gctaccacaa tctttacttc
    11401 ttgcttaacc tgatgaagca agtgcgacaa gccatcatgg atgacaatct cttggaattc
    11461 cgtgagtatt ttgtggaaaa atatggctat aataagtcag gacgtaattt ctaaaatgga
    11521 attgatataa aaaaatccta agttttctct taggattttt cttctttttt tgatagaata
    11581 aagtgtacaa tgaaaggaag aataaactcg tatgcgcatt aaatggtttt ccttgattag
    11641 gattataggt ttacttttgg tactcttgta tcacttcttt cagacgatct ttcctggagg
    11701 atttttcggg gtagatgtct ttttcacatt ttcaggtttc ctgattacag ctctactcat
    11761 tgaagaattt tctaagaaca atgagattga tttgatagga ttttttagaa gacgctttta
    11821 tcggattgtg ccacctgtgg ttttgatggt cttggtgacc atgcctttta ctttcttggt
    11881 tcgccaagac tatgttgctg gaattggtgg ccagattgcg ggcgtcttag gctttatgac
    11941 caacttctat gaactcctaa caggtgggag ttatgaatct cagttcattc ctcatttgtt
    12001 tgttcataat tggagtttgg cagttgaggt tcactactat attctttggg gattggcagt
    12061 ttggttctta tccaaacaag ctaaatcaaa tggtcagttg aaggggatgg tctttctctt
    12121 atctgctgct gccttcttga tcagtttctt ctccatgttt attggtagtt ttctagtgac
    12181 ctcttattcc tctgtttatt tctccagttt aactcatgtc tatccattct ttttgggaag
    12241 tatgttagca actattgtag gtgttcgtca gacgacttcc ctcgtcaagc agttggataa
    12301 aatctgggat ttacgaaaga ctttggtagt ttttggagga ggctttggtt tcctagttct
    12361 tttgactttc tttgtcaaat tcacttatct ttttgcctat cttatcggct tcttgcttgc
    12421 cagtcttgca gctcttgcca tgattctggc ggcgcgtgtc ttacatgaaa agacacatca
    12481 tatacaggag ccgaagatta tcagcttttt agcggatact agctatgcgg tttatctttt
    12541 ccattggcct ttctatatca ttttctcaca gttgacatca aatcttcttg ctgtattact
    12601 gactctgatt tgttcttatg gctttgccag tctgtcattc tatgtattgg aaccttggat
    12661 tgcaggcaag aacacaccta ttttacaaac tcttcgtccc ctgccttata ttcacgcaat
    12721 tcttgtagca ggtacaggaa tcttgaccat cattgtctgc acggtgacct tgttggcacc
    12781 acaagtggga gcgtttgaga cagacttgac tgtcaatggc ttgaagcaag ctgcaacaaa
    12841 tattggccag accaaggtga tggcagaacg ggcagatgca aacagtttgg gaattgctga
    12901 tggcactatg ttaattggtg actcagtggc tttaagggca aatacagcac tacagacagc
    12961 tcttcctgga gcacagatta acgcgcaggt cagcgtaaca accaagaccg caaatgaaat
    13021 catgctaaat aatagccaga ataaattttt acctaagacg gtggtcattg cgactggggt
    13081 aaataatcct gagaattaca aggatgactg ggacagtatc gtgaaaaatc ttcctaaggg
    13141 acaccatatg attttggtga ctccttatga gggagataag acaaaagaga cctatgccat
    13201 cgttgagaag gctgctgcct atatgagaga attggcagag aagacacctt acattacgat
    13261 agcagattgg aatcaagttg cgaaagagca tccagaaatt tgggctggaa cagaccaggt
    13321 tcatttcggg agtgagagta gcactatcga agcaggagca aaattgtatg cagatacgat
    13381 tgccacagct ttgcagacag ctcaagacaa gccggttaaa tcaaaataac ttgtattaaa
    13441 aaagagaaga gttggagaaa tccagctctt ttaaaatatc tccaaaaaat aggtctatac
    13501 catttacaaa taaaaaagaa aggtttataa tgtaattgac ataataaatt gtagaatcaa
    13561 tcttttaagg aggttaacat tatgcctaac tatattaaag cggatcagtt tttctaccca
    13621 cacggagttc gtcgaggtgg ttacttggaa cttgtggacg gcaagtttgg gaaacatgta
    13681 gagcagattc ctgaaggggc tgaggtgatt gactatacag gttatagcat tgccccaggt
    13741 cttgtggata ctcatattca tggatatgca ggtgtagatg tgatggacaa caacattgaa
    13801 ggtacattgc atactatgag tgaaggactt cttagtaccg gtgttaccag tttcttaccc
    13861 acaactttaa cagccactta tgagcaattg cttgcagtca ctgaaaatct tggaaaccat
    13921 tataaagaag caacaggtgc taagattcgt gggatttatt atgaaggtcc atatttcaca
    13981 gaaactttta agggggcaca aaatccaact tatatgagag acccgggtgt tgaggagttt
    14041 cattcttggc aaaaagcggc aaatggcttg cttaataaaa ttgcccttgc accagaacgt
    14101 gatggggtgg aagactttgt tcgtacagtt acgggcgaag gtgtgacggt tgctcttgga
    14161 cattcaaacg cgacttttga tgaagccaaa aaagcagtcg atgctggagc gagtgtttgg
    14221 gtgcatgcct acaatggaat gcgtgggttg actcaccgtg aattgggtat ggttggagcc
    14281 atgtaccaat tgccacatac ctatgcagag ttgatctgtg atggtcacca cgtagatcca
    14341 aaggcttgcg aaattcttat caaacaaaaa ggaacagaaa acatcgctct tatcacagac
    14401 tgtatgacag ctgggggatt ggaagacgga gattatatgt tgggagaatt cccagttgtc
    14461 gttgcaaatg gaactgcacg cctcaaatcg acaggtaact tggcaggttc tatcctcaaa
    14521 ctcaaagatg gtttgaagaa tgtggtcgaa tggggaattg cgaatccgca tgaagcagtc
    14581 atgatggcca gcttcaaccc agctaaatcc gttcacatcg atgacgtctg tggccaaatc
    14641 cgtgaaggct acgacgctga cttcatcgta ttagataaag atttggaatt ggtagcaacc
    14701 tatctagatg gcgtaaaacg ttatcaagca taagagagaa ggcagaagtt agagactagc
    14761 ttctgctttt ttatacaggg tactttactg gtaaataaaa agttaaaaaa atcacaaaaa
    14821 aagcttgaag aaacaaagaa aaaggcttac aattgaacta taaatattac aaataaaaaa
    14881 acggaggagt gctttatgaa agcctatact tatgttaaac caggacttgc ttcttttgtt
    14941 gatgtagaca aaccagttat tcgcaagcca acagacgcta ttgtgcgtat tgtaaaaacc
    15001 actatttgtg gaacagacct ccatattatc aaaggggatg ttcctacttg ccaaagtggt
    15061 accattcttg gccacgaagg gattgggatt gttgaagaag ttggggaagg agtttccaac
    15121 ttcaaaaaag gcgacaaggt cttgatttct tgcgtctgtg cctgtggtaa atgctactac
    15181 tgtaaaaaag gaatttatgc ccactgtgaa gacgaagggg gctggatttt cggtcacttg
    15241 attgatggta tgcaggctga atatctacgt gtccctcatg cagataatac tctttaccat
    15301 actccagaag acttgtcaga tgaagctttg gttatgctgt cagacattct gcctactgga
    15361 tatgaaattg gtgtcttaaa agggaaagta gaacctggtt gcagcgtagc cattattggt
    15421 tcaggtccag ttggattggc tgctctttta acagcccaat tctattcacc agctaaattg
    15481 attatggtag acctagacga taaccgcttg gaaactgccc tatcattcgg tgcgactcat
    15541 aaggttaatt cttcagaccc taaaaaagcc attaaagaaa tttatgattt gacagatggt
    15601 cgtggtgtgg atgtcgctat cgaagctgta gggattcctg aaacatttga tttctgtcaa
    15661 aagattatcg gtgtagacgg aacggttgcc aactgtggtg tgcatggtaa accagttgaa
    15721 ttcgatttag ataaactttg gattcgcaac atcaatgtaa caactggttt ggtatctaca
    15781 aatacgactc cacaattgtt gaaagcactt gaaagtcata agattgaacc ggaaaaattg
    15841 gtaactcact atttcaaact cagtgaaatt gaaaaagcct acgaagtctt cagtaaggca
    15901 gcagaccacc atgccattaa ggtcattatc gaaaacgata tctcagaagc ctaagtagta
    15961 aaaatatttt tgtacataag taaatagaaa ttcagtcatc catcagatgg ttggattttt
    16021 tatcaaaaaa ttaagaaatg agcatatttc tttccttgtc tggcggaatt ggttataata
    16081 tacggtacaa aggaatgaat gaatatgtat cgtgttatag aaatgtacgg agattttgaa
    16141 ccgtggtggt tcttagaagg ttgggaagaa gatattgtag caagtagaaa atttgaccag
    16201 tattatgatg ctctcaaata ctacaaaact tgctggttta gattggaaca agaatcgcct
    16261 ctttataaaa gtagaagcga cttgatgacc attttttggg acccggaaga ccaacgctgg
    16321 tgtgatgaat gtgatgagta tttacaacaa taccattctt tggctctttt gcaggatgag
    16381 caggttatcc cagacgaaaa actacgctca ggctatgaaa aacaaaccag tcaggaaagg
    16441 aatcgttctt gccgtatgaa attaaaatag agaaaagtaa ctttttggag ttgctttttt
    16501 tatttttcta acgctttgcg aatagtatag gtgaggaggt aagtatggtt caagaaattg
    16561 cacaagaaat cattcgttcg gctcggaaaa aaggggcgca agacatttat tttgtcccca
    16621 agttagatgc ctatgagctt catatgaggg taggagacga gcgctgtaaa attggtagct
    16681 atgattttga aaagtttgca gccgttatca gtcactttaa gtttgtggcg ggtatgaatg
    16741 tgggagaaaa aagacgtagt caactgggtt cctgtgatta tgcctatgac cagaagatgg
    16801 cgtctctacg tttatctact gtaggcgatt atcgggggca tgagagtttg gttatccgtt
    16861 tgttgcacga tgaggagcag gacttgcatt tttggtttca ggatattgaa gaattaggca
    16921 agcagtacag gcaacgggga ctctatcttt ttgctggtcc ggttgggagt ggtaagacga
    16981 ccttgatgca tgaattgtcc aagtcactct ttaaaggaca gcaagttatg tccatcgaag
    17041 atcctgtcga aatcaagcag gacgacatgc ttcagttgca gttgaacgaa gcaatcggcc
    17101 taacctatga aaatctaatc aaactttcct tgcgtcatcg accagatctc ttgattatcg
    17161 gagaaattcg tgacagcgag acggcgcgtg cagtggtcag agctagtttg acaggtgcga
    17221 cagtcttttc aaccattcac gccaagagta tccgaggtgt ttatgagcgt ctgctggagt
    17281 tgggtgtgag tgaagaagaa ttggcagttg ttctgcaagg agtctgctac cagagattaa
    17341 tcgggggagg aggaatcgtt gactttgcaa acagagatta tcaagaacac caagcagcca
    17401 agtggaatga gcaaattgac cagcttctta aagatggaca tatcacaagt cttcaggctg
    17461 agacggaaaa aattagctac agctaagcaa aaaaatatca tcaccctatt taacaatctc
    17521 ttttctagcg gttttcatct ggtggagact atctcctttt tagataggag ttccttgttg
    17581 gacaagcagt gtgtgaccca gatgcgtaca ggcttgtctc aggggaaatc attctcagaa
    17641 atgatggaaa gtttgggatg ttcaagtacc attgtcactc agttatctct agctgaagtt
    17701 catggcaatc ttcacctgag tttgggaaag atagaagaat atctagacaa tctggccaag
    17761 gtcaagaaaa agttaattga agtagcgacc tatcctttga ttttgctggg ttttcttctc
    17821 ttaattatgc tggggctacg gaattacctg ctcccacaac tggatagtag caatattgcc
    17881 acccgaatta tcggtaatct gccacaaatt tttctaggca tggtagggct tgtttccgtg
    17941 cttgcccttt tagcactaac tttttataaa agaagttcta agatgagtgt cttttctatc
    18001 ttagcacgcc ttccctttat tggaatcttt gtgcagacct acttgacagc ctattatgca
    18061 cgtgaatggg ggaatatgat ttcacaggga atggagctga cgcagatttt tcaaatgatg
    18121 caggaacaag gttcccagct ctttaaagaa atcggtcaag atctggctca aaccctgaaa
    18181 aatggccgtg aattttctca gacgatagga acctatcctt tctttaggaa ggaattgagt
    18241 ctcatcatag agtatgggga agttaagtcc aagctgggta gtgagttgga aatctatgct
    18301 gaaaaaactt gggaagcctt ttttacccga gtcaaccgca ccatgaattt ggtgcagcca
    18361 ctggttttta tctttgtggc actgattatc gttttacttt atgcggcaat gctcatgccc
    18421 atgtatcaaa atatggaggt aaatttttaa aatgaaaaaa atgatgacat tcttgaaaaa
    18481 agctaaggtt aaagctttta cattggtgga gatgttagta gtcttgctga ttatcagcgt
    18541 gcttttcttg ctctttgtac ctaatctgac caagcaaaaa gaagcagtca atgacaaagg
    18601 aaaagcagct gttgttaagg tggtggaaag ccaggcagaa ctttatagct tggataaaaa
    18661 tgaagatgct actctgagca agttacaagg agatgggcgc atcacggaag aacaggttaa
    18721 agcttataat gaatactata ctaaaaatgg aggggcaaat cgtaaagtca atgattaagg
    18781 cctttaccat gctggaaagt ctcttggttt tgggacttgt gagtatcctt gccttgggct
    18841 tatccagctc tgtccagtcc acttttgcag cagtagagga gcagattttc tttatggagt
    18901 ttgaagaact ctatcgggaa acccaaaaac gcagtgtagc cagtcagcaa aagactagtc
    18961 taaatttaga tgggcagacg cttagcaatg gcagtcaaaa gttgacagtt cctaaaggaa
    19021 ttcaggcacc atcaggccaa agtattacat ttgaccgagc tgggggcaat tcgtccctgg
    19081 ctaaggttga atttcagacc agtaaaggag cgattcgcta tcaattatat ctaggaaatg
    19141 gaaaaattaa acgcattaag gaaacaaaaa attagggcag tgattttact ggaagcagta
    19201 gtcgctctag ctatctttgc cagcattgcg accctccttt tgggacaaat tcaaaaaaat
    19261 aggcaagagg aagcaaaaat cttgcaaaag gaagaagtct tgagggtagc taagatggcc
    19321 ctgcagacgg ggcaaaatca ggtaagcatc aacggagttg agattcaggt attttctagt
    19381 gaaaaaggat tggaggtcta ccatggttca gaacagttgt tggcaatcaa agagccataa
    19441 ggtcaaggct tttaccttgt tagaatccct gcttgccctc attgtcatca gtgggggatt
    19501 actccttttt caaactatga gtcagctcct catttcagaa gttcgctacc agcaacaaag
    19561 cgagcaaaag gagtggctct tgtttgtgga ccaacttgag gcagaattag accgttcgca
    19621 gttcgaaaaa gtagaaggca atcgcctata catgaagcaa gatggcaagg acatcgccat
    19681 cggtaagtca aagtcagacg attttcgtaa aaccgatgct agtggacggg ggtatcagcc
    19741 tatggtttat ggcctcaaat cagctcagat tacagaggac aatcaactgg ttcattttcg
    19801 tttccagttt caaaaaggct tagaaaggga gttcatctat cgtgtggaaa aagaagaaag
    19861 ttaaggcagg tgttctcctc tacgcagtca ccatagcagc catctttagt cttttgttgc
    19921 aattttattt gaaccgacaa atcgcccact atcaagacta tgctttaaat aaagaaaaat
    19981 tggttgcttt tgccatggct aaacgaacca aggataaagc cgagcaagaa agtggggaac
    20041 aggtttttaa tctaggtcag gtaagctatc aaaacaagaa aacaagcttg gtgacgactg
    20101 ttcgtacgtc taagagccaa tatgagtttc tgtttccttc agtcaaaatc aaagaagaga
    20161 aaagagataa aaaggaagag gtagcgaccg attcaagcga aaaagcggag aaaaaaaatc
    20221 agaagagaag cctgaaaaga aagagaattc ctagtcaatt caactataat gcgttgaatc
    20281 cagaatagtc cactgtagtt tctagaaaat tgctggaaat ggatgttaag ctccaattca
    20341 tttgtttata tcttatttca gtttactata ctttgtgcta aactaaagat atgaaacatg
    20401 attttaacca caaagcagaa actttcgact cccctaaaaa tatcttcctc gcaaacttgg
    20461 tatgtcaagc agtcgagaaa cagattgata ttttatcaga caaagtaatt ttagatttcg
    20521 gtggtggcac gggtctatta gccttgcccc tagccaagca ggctaagtca gttactcttg
    20581 tagacatttc tgagaaaatg ggctctataa tatttgtagt gggtaaatcc actat
//
LOCUS       DAYYQN010000038        20007 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYYQN010000038 DAYYQN010000000
VERSION     DAYYQN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.038"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..385
                     /locus_tag="ACHBRQ_001366"
     CDS             <1..385
                     /locus_tag="ACHBRQ_001366"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9593018.1"
                     /translation="KMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEFDCLAVSRVLH
                     HMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIEHGFSSVHSQI
                     LYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            446..1399
                     /locus_tag="ACHBRQ_001367"
     CDS             446..1399
                     /locus_tag="ACHBRQ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9593019.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1450..2640
                     /locus_tag="ACHBRQ_001368"
     CDS             1450..2640
                     /locus_tag="ACHBRQ_001368"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFM9593020.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2641..2772
                     /locus_tag="ACHBRQ_001369"
     CDS             2641..2772
                     /locus_tag="ACHBRQ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593021.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            2789..3160
                     /gene="rnpA"
                     /locus_tag="ACHBRQ_001370"
     CDS             2789..3160
                     /gene="rnpA"
                     /locus_tag="ACHBRQ_001370"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9593022.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVN"
     gene            3135..3959
                     /locus_tag="ACHBRQ_001371"
     CDS             3135..3959
                     /locus_tag="ACHBRQ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9593023.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3978..4964
                     /gene="jag"
                     /locus_tag="ACHBRQ_001372"
     CDS             3978..4964
                     /gene="jag"
                     /locus_tag="ACHBRQ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9593024.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            5015..5638
                     /locus_tag="ACHBRQ_001373"
     CDS             5015..5638
                     /locus_tag="ACHBRQ_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFM9593025.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5769..7226
                     /locus_tag="ACHBRQ_001374"
     CDS             5769..7226
                     /locus_tag="ACHBRQ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9593026.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLAAYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7249..7530
                     /locus_tag="ACHBRQ_001375"
     CDS             7249..7530
                     /locus_tag="ACHBRQ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9593027.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQRDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7606..8091
                     /locus_tag="ACHBRQ_001376"
     CDS             7606..8091
                     /locus_tag="ACHBRQ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9593028.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8108..8773
                     /locus_tag="ACHBRQ_001377"
     CDS             8108..8773
                     /locus_tag="ACHBRQ_001377"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9593029.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8777..9640
                     /locus_tag="ACHBRQ_001378"
     CDS             8777..9640
                     /locus_tag="ACHBRQ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9593030.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9642..10346
                     /locus_tag="ACHBRQ_001379"
     CDS             9642..10346
                     /locus_tag="ACHBRQ_001379"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9593031.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10525..12198
                     /locus_tag="ACHBRQ_001380"
     CDS             10525..12198
                     /locus_tag="ACHBRQ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9593032.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEQEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12310..13401
                     /gene="ulaG"
                     /locus_tag="ACHBRQ_001381"
     CDS             12310..13401
                     /gene="ulaG"
                     /locus_tag="ACHBRQ_001381"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9593033.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13515..15491
                     /gene="tkt"
                     /locus_tag="ACHBRQ_001382"
     CDS             13515..15491
                     /gene="tkt"
                     /locus_tag="ACHBRQ_001382"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9593034.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15608..15907
                     /gene="yajC"
                     /locus_tag="ACHBRQ_001383"
     CDS             15608..15907
                     /gene="yajC"
                     /locus_tag="ACHBRQ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9593035.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15953..16381
                     /locus_tag="ACHBRQ_001384"
     CDS             15953..16381
                     /locus_tag="ACHBRQ_001384"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9593036.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16383..16793
                     /locus_tag="ACHBRQ_001385"
     CDS             16383..16793
                     /locus_tag="ACHBRQ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFM9593037.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILTVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17093..19744
                     /gene="adhE"
                     /locus_tag="ACHBRQ_001386"
                     /gene_synonym="adhC"
     CDS             17093..19744
                     /gene="adhE"
                     /locus_tag="ACHBRQ_001386"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9593038.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6260 a         3598 c         4361 g         5788 t
ORIGIN      
        1 gaaaatgttg gagcaagctc gtttgaaagt ggagcagcaa gcaatcaaga atatccagtt
       61 tttggagcaa gatttaccga aaaatccctt ggagaaagag tttgattgcc ttgctgttag
      121 tcgggttctt catcatatgc ctgatttgga tgcggctctc tcactgtttc atcaacattt
      181 gaaggaagat gggaaactca tcattgctga ttttaccaag acagaagcta atcatcatgg
      241 atttgattta gctgaactgg aaaacaagct aattgagcat ggtttttcat ctgtgcatag
      301 tcagattctc tatagcgctg aagacctgtt tcaaggaaat cactcagaat tctttttaat
      361 agtagcccaa aaatcactcg cctagtcagg gagtgatttt tctataagga tggaaaaaag
      421 aagggaaatt tgataagata ggaatatgga ttttgaaaaa attgaacaag cttataccta
      481 tttactagag aatgtccaag ttatccaaag tgatttggcg accaactttt atgacgcctt
      541 ggtggagcaa aacagcatct atctggatgg tgaaactgag ctaaaccagg tcaaagacaa
      601 caatcaggcc cttaagcgtt tagcactacg caaagaagaa tggctcaaga cctaccagtt
      661 tctcttgatg aaggctgggc aaacagaacc cttgcaggcc aatcaccagt ttacaccaga
      721 tgccattgct ttgcttttgg tgtttattgt ggaagagttg tttaaagagg aggaaattac
      781 tatcctcgaa atgggttctg ggatgggaat tctaggcgct actttcttga cctcgcttga
      841 taaaaaggtg gattacttgg gaatggaaat ggatgatttg ctgattgatc tggcagctag
      901 catggcagat gtaattggtt tgcaggctgg ctttgtccaa ggagatgccg ttcgtccaca
      961 aatgctcaaa gaaagcgatg tggtcatcag tgacttgcct gttggctatt atcctgatga
     1021 tgccgttgcg tcgcgccatc aagttgcttc tagccaagaa catacttacg cccatcactt
     1081 gctcatggaa caagggctta agtacctcaa gtcagacgga tacgctattt ttctagctcc
     1141 gagtaatttg ttgaccagtc ctcaaagtga tttgttaaaa gaatggctga aagaagaggc
     1201 gagtctggtt gctatgatta gtctgcctga aaatctcttt gctaatgcca aacaatctaa
     1261 gactattttt atcttacaga agaaaaatga aatagcagta gagccttttg tttatccact
     1321 tgctagcttg caagatgcaa gtgttttaat gaaatttaaa gaaaattttc aaaaatggac
     1381 tcaaggtact gaaatataaa atagattttg ttataatagt tgaaaacgct taaaaagggg
     1441 tatcatgtta tgacaaaaac aattgcaatc aatgcaggaa gttcaagttt gaaatggcaa
     1501 ttatacttaa tgccagaaga aaaagtgtta gccaaaggtt tgattgaacg tatcggtttg
     1561 aaagattcaa tttcaactgt aaaatttgac ggccgttctg agcaacaaat tttggatatt
     1621 gaaaatcata cacaagccgt taaaatttta ttggatgact tgattcgttt cgatattatc
     1681 aaggcttatg acgagattac aggtgttgga catcgtgttg ttgctggtgg agaatatttc
     1741 aaagaatcaa cagttgttga gggagatgtt ttagaaaaag ttgaagagtt gagtttgttg
     1801 gctcctctac acaacccggc caatgcagca ggtgttcgtg ccttcaagga attgttgcca
     1861 gacattacca gtgtagttgt ttttgatact tccttccaca caagtatgcc agagaaagct
     1921 tatcgctacc ctctaccaac aaaatattac acagaaaaca aggttcgtaa atacggtgct
     1981 catggtacaa gtcaccagtt tgtagcagga gaagctgcaa aactcttggg acgtccatta
     2041 gaagacttga agttaattac ctgtcatatt ggtaacggag gctcaattac agctgtgaaa
     2101 gccggcaaat ctgtagacac ttctatgggg ttcactcctc ttggtggtat tatgatggga
     2161 acgcgtacag gggatattga tccagctatc attccttatt taatgcaata tacagaggat
     2221 tttaacacac cagaagatat cagtcgtgtt cttaaccgtg aatcaggtct tttgggagtt
     2281 tctgctaatt ctagcgatat gcgcgatata gaagcagctg tagcagaagg gaatcacgag
     2341 gctagcttgg cttatgaaat gtatgttgac cgtatccaaa aacatatcgg tcagtacctt
     2401 gcagtgctaa atggagcaga tgccattgtt ttcacagcag gtgtcggtga aaatgcagag
     2461 aatttccgtc gtgatgtaat ctcagggatt tcgtggtttg gttgtgatgt tgatgatgaa
     2521 aagaatgtct ttggcgttac aggagacatc tcaacagagg cagctaaaat ccgtgtcttg
     2581 gttattccaa cagatgaaga attagtcatt gcccgtgacg ttgaacgctt gaaaaaataa
     2641 gtgaaactaa aaaaatattc aatacaagga gttgggaaag ttatttttcc agcttctttt
     2701 tctgatgaaa ttgtccaaaa ccttgctatg attggctttt ttgaaaaata tggtataata
     2761 gtagtaattt aatagatgga gttgagtttt gaagaaaaac tttcgtgtaa aaagagagaa
     2821 agattttaag gcgattttca aggaggggac aagttttgct aatcgcaaat ttgtggtcta
     2881 ccaattagaa aaccagaaaa accattttcg agtaggtcta tcagttagca aaaaactggg
     2941 gaatgccgtc actagaaatc aaattaagcg acggattcgg catattatcc agaatgcaaa
     3001 agggagtctg gtagaagatg tcgactttgt tgtcattgct cgaaaaggag tcgaaacctt
     3061 gggatacgca gagatggaga aaaatctact ccatgtatta aaattatcaa agatttaccg
     3121 ggaaggaaat gggagtgaaa aagaaactaa agttaactag tttgctagga ctgtctctgt
     3181 taatcatgac agcctgtgcg actaatgggg taactagcga tattacagcc gaatcggctg
     3241 atttttggag taaattggtt tacttctttg cggaaatcat tcgcttttta tcgtttgata
     3301 ttagtatcgg agtggggatt attctcttta cggtcttgat tcgtacagtc ctcttgccag
     3361 tctttcaggt gcaaatggtg gcttctagga aaatgcagga agctcagcca cgcattaagg
     3421 cgcttcgaga acaatatcca ggtcgagata tggaaagcag aaccaaacta gagcaggaaa
     3481 tgcgtaaagt atttaaagaa atgggtgtca gacagtcaga ctctctttgg ccgattttga
     3541 ttcagatgcc ggttattttg gccctgttcc aagccctatc aagagttgac tttttaaaga
     3601 caggtcattt cttatggatt aaccttggta gtgtggatac aacccttgtt cttccgattt
     3661 tagcagcagt attcaccttt ttaagtactt ggttgtccaa caaagctttg tctgagcgaa
     3721 atggcgctac gactgcgatg atgtatggga ttccagtctt gatttttatc tttgcagttt
     3781 atgcgccagg tggagtcgcc ctatactgga cagtgtctaa tgcttatcaa gtcttgcaaa
     3841 cctatttctt gaataatcca ttcaagatta tcgcagagcg cgaggccgta gtacaggcac
     3901 aaaaagattt ggaaaataga aaaagaaaag ccaagaaaaa ggctcagaaa acgaaataaa
     3961 taaggaggaa tctggtaatg gtagtattta caggttcaac tgttgaagaa gcaatccaga
     4021 aaggattgaa agaattagat attccaagaa tgaaggctca tatcaaagtc atttctaggg
     4081 agaaaaaagg ctttcttggt ctatttggta aaaaaccagc ccaagtggat attgaagcga
     4141 ttagtgaaac gactgttgtc aaagcaaatc aacaggtagt aaaaggcgtt ccgaaaaaaa
     4201 tcaatgattt gaacgagcct gtgaagacgg ttagtgaaga aaccgttgac cttggtcatg
     4261 tggttaatgc tattaaaaaa atagaggaag aaggtcaagg tatttctgat gaagtcaagg
     4321 ctgaaatctt aaaacatgaa agacatgcca gcactatctt agaagaaact ggtcacattg
     4381 agattttaaa tgaacttcaa atcgaggaag cgatgaggga agaagcaggc gctgatgacc
     4441 ttgaaactga gcaagatcaa actgaaaatc aagacttgaa agagatgggc ttgaaggtcg
     4501 agcaaagtta tgatattgcc caggtggcta cggatgtgac tgcctatgtt caagcgattg
     4561 tggatgacat ggatgttgag gctacacttt caaatgatta taaccgtcgt agcatcaatc
     4621 tacaaattga caccaacgaa ccaggtcgta ttatcggcta ccatggtaaa gtcttgaagg
     4681 ccttgcaact gttggctcaa aattatcttt acaaccgcta ttccaaaacc ttctacgtta
     4741 caatcaatgt caatgattat gtcgaacacc gtgcagaagt cttgcagacc tatgcgcaaa
     4801 aattggcgaa tcgtgttttg gaagaaggtc gcagtcataa aacagatcca atgtcaaata
     4861 gcgaacgcaa gattatccat cgtattattt cacgtatgga tggcgtgact agttactctg
     4921 aaggtgatga gccaaatcgc tatgttgttg tagatacaga ataagtaaaa tcagggttga
     4981 cctgattttt tgctagttag aggaggttaa actgatgttg aataagataa gagactatct
     5041 agacttttct ggtttgcagt accgtagtcc tgataaagcg ggagcagagc gagagaagat
     5101 gctggcgttc cgccacaaag gacaagaggc ccgaaaggct tttacagaac tggccaaagc
     5161 ctttcaagca agccatccag aatggcaact ccaacagact agtcagtgga tgaatcaggc
     5221 ccagcgtttg agaccacatt tttgggttta tctacagaga gacggacaag tgacagaacc
     5281 tatgatggcc ttacgtttgt atgggacatc tactgacttt ggaatttctt tggaagtcag
     5341 tttcatcgaa cgtaagaagg atgagcaaac actgggcaag caggccaaag ttttagacat
     5401 tccaaccgtt aaagggattt attatctaac ctactctaat ggtcaaagtc aacggtggga
     5461 ggcgaatgaa gaaaagcgtc gtactttacg cgagaaggtg agaagtcaag aagttcgaaa
     5521 agttttagtg aaggtagatg ttcctatgac agaaaattcg tctgaagaag aaatcgtaga
     5581 aggcttattg aagtcttatt ctaaaattct tccctattat ctagctacga gaaaataaga
     5641 taatttgtaa aacatcataa atcatacagt ccaagagtga acagtccgct gtgtaattct
     5701 tggtcttttt gtttgcgctt tcgcattata taataaactt acaaaaacaa ttcaaaagga
     5761 gaacaattat ggaagtcgtt tcaagtgttc taaattggtt ttctagcaat attttgcaga
     5821 atcccgcatt tttcgtaggt ttattggtgt tgataggata tgcacttttg aaaaaacctg
     5881 cccatgacgt tttttcaggg tttgttaaag caacagtagg gtatatgttg cttaacgtgg
     5941 gtgctggtgg tttggttaca acctttcgtc caatcttagc agctcttaac tacaaattcc
     6001 aaattggtgc agcggttatc gacccttatt ttggacttgc tgcagcaaac aacaaaattg
     6061 tagcagagtt tccagatttt gttggaactg caactacagc tctattgatt ggttttggaa
     6121 taaatatctt gctcgtagct cttcgaaaga ttacgaaggt aagaaccctc tttattactg
     6181 gtcacatcat ggtacaacaa gctgcaacag tatctcttat ggttctattc ttagtaccac
     6241 aattgcgcaa tgcttacggt acagcagcga ttggtatcat ctgtggactt tactgggcag
     6301 ttagttcaaa tatgactgtt gaggcaactc aacgcttgac tggtggtggc ggatttgcga
     6361 ttggtcacca acagcaattt gcaatctggt ttgtagataa agtagcagga cgctttggta
     6421 agaaagaaga aagtttagac aatcttaaat tacctaagtt cctctcaatc ttccacgata
     6481 cagttgttgc atctgctacc ttgatgctcg tattcttcgg agccattctt ttaatcttgg
     6541 gtccagacat tatgtctaat aaagaagtca tcacttcagg aactctattc aatcctgcta
     6601 aacaagattt ctttatgtac attatccaaa cagcctttac cttctcagtt tacttgttcg
     6661 ttttgatgca aggtgtccga atgttcgtat ctgagttgac aaacgccttc caaggtattt
     6721 caaacaaatt gttgccaggt tcattcccag cggttgacgt tgcagcttct tatggatttg
     6781 gttctccaaa tgctgtcttg tcaggattta cctttggttt gattggtcaa ttgattacaa
     6841 ttgtcttgct catcgtcttt aaaaatccga ttcttattat tacaggattt gtaccagtgt
     6901 tctttgacaa tgcagccatt gcggtctacg ctgataaacg cggcggatgg aaagcggctg
     6961 ttatcctttc ctttatatca ggtgtccttc aagttgctct aggagctctt tgtgtggccc
     7021 ttctcgattt ggcagcttat ggtggctacc atggaaatat cgactttgaa ttcccatggc
     7081 ttggatttgg atatatcttc aaataccttg gtattgttgg ttatgtactt gtgtgtctct
     7141 tcttgcttgt tattcctcaa cttcaatttg ccaaagcaaa agataaagag aaatattaca
     7201 acggtgaagt tcaagaagaa gcttagtatc tagaaaagga gaaataaaat ggttaaagta
     7261 ttagcagcgt gcggaaatgg aatgggttca tcaatggtta tcaagatgaa ggttgaaaat
     7321 gctctccgta agcttaatca aagagatttt acagtcaatt catgcagtgt cggtgaagct
     7381 aaaggtttag cagtaggata tgacatcgta atcgcttctc ttcatttgat tcaagaattg
     7441 gaagggcgaa ctaatgggaa gttaattggg cttgataact tgatggatga taaagaaatc
     7501 accgaaaaac tcagtcaagc actacagtaa aaggttggag ggggctggac agaaactgag
     7561 agttatcgtt tctgtccttc tccctcttaa ataaaggagg cagatatgaa tttaaaacaa
     7621 gctttaattg acaatgactc gatccgacta ggtttagagg ctaacaattg gaaagaagca
     7681 gtcaaggtag cagtagatcc cttaattgaa agtggggcaa ttttgccaga gtattacgat
     7741 gctatcattg aatcgactga agagtatggg ccttactata tcttgatgcc aggtatggct
     7801 atgccccacg ctagacctga agctggtgtg caaagtgatg ccttttcatt gattacctta
     7861 caaaatcctg ttgtattttc agatgggaaa gaggtatctg ttttgttggc actagcagca
     7921 acaagctcaa aaattcacac aagtgtagcc attccacaaa ttattgccct gtttgaatta
     7981 gaagattcta ttgcacgttt acaggcttgc cagactaaag aagatgtctt ggctatgatt
     8041 gaagaatcta aggatagccc ttatctcgaa ggattggatt tggaaagtta gaaagaggaa
     8101 taaagaaatg acaaaaagaa tacctaattt acaagttgca ttagaccatt cagacttgca
     8161 aggagcgatt aaagcagctg tttctgttgg tcaggaagta gatattatcg aagctggaac
     8221 tgtttgcttg cttcaagttg gaagtgaact ggctgaagtc ttgcgtagcc ttttcccaga
     8281 taagattatt gtggcagaca caaaatgtgc tgatgctggt ggaacagttg ctaaaaataa
     8341 tgcggttcgt ggagcagact ggatgacttg tatctgttgt gcaaccatcc ctactatgga
     8401 agcagctcta aaggctatca agactgaacg aggagaacga ggcgaaatcc agatcgagct
     8461 ttatggcgat tggacttttg aacaagctca gctttggcta gatgcaggta tctcacaagc
     8521 tatttatcac caatctcgtg atgctcttct tgctggtgaa acttggggtg aaaaagacct
     8581 taataaggtt aaaaaactca ttgacatggg cttccgtgta tctgtaacag gtggtctaga
     8641 tgtagatact ctcaaactct ttgaaggtgt tgatgtcttt acctttatcg caggtcgtgg
     8701 aattacagag gctgcggatc cagcaggagc agcgcgtgcc ttcaaggatg aaatcaaacg
     8761 aatttggggg taaatcatgg tacgtccaat tggaatttat gaaaaggcaa ccccaacaca
     8821 ctttacttgg ctagaacgtt taaattttgc caaggagtta ggctttgatt ttgtcgagat
     8881 gtctattgac gaacgtgacg agcgtttagc aagacttgac tggagtaagg aagaacgctt
     8941 ggaagttgtc aaagcaatct atgaaactgg tgttcgtatt ccttctatct gtttttcagg
     9001 ccatcgtcgc tacccattgg gttcaaaaga tccagttcta gaggaaaaat ctctagaact
     9061 catgaaaaaa tgtatcgaat tagctcaaga cttgggagtt cgtacgattc aattagctgg
     9121 ttacgatgtt tactatgagg aaaagtcacc ccagacacgc caacgtttta tcaaaaattt
     9181 gagaaaagcc tgtgactggg ctgaagaagc tcaggtggta cttgctattg aaattatgga
     9241 tgatcctttc atcagtagca tcgaaaaata tttggctata gaaaaagaga ttgactctcc
     9301 cttcctcttt gtatatccag atattggtaa tgtgtctgca tggcataatg atatctatag
     9361 tgagttttat cttggtcatc atgccatcgc agctctccat ctcaaggata cttatgcagt
     9421 gacagaaagt tcaaggggcc agttccgaga tgtacctttt gggcaaggtt gtgtcaaatg
     9481 ggaagaagct ttcgatattt taaaggaaac caattataat ggacctttcc taatcgaaat
     9541 gtggtctgaa aattgtgaaa cagtagaaga aacacgcgca gccattcaag aggcgcaagc
     9601 ttttctctat ccactcatta agaaagcagg tttgatgtaa gatgaatcaa gtaatcaatg
     9661 ctatgcgtaa acgagtctgt gatgccaatc aatcattgcc aaaacatgga cttgtcaaat
     9721 ttacctgggg gaatgtatct gaagtcaatc gcgaactcgg tgtcattgtt atcaaaccat
     9781 caggcgtgga ttatgacgaa ttgacacctg aaaacatggt agtgactgat ctagatggta
     9841 agatcctaga aggggattta agaccatctt ccgacctccc aactcatgtg caattatata
     9901 gggcttggtc agaaattggt agtgtggttc acacccattc gacagaagct gttggttggg
     9961 ctcaggcagg tcgtgatatt cctttctacg gaacaaccca tgcagattat ttctacggtt
    10021 caatcccttg cgcccgtagt ttgaccaagg acgaagtaga agtggcctat gaaaaagata
    10081 ctggcctggt tatcgtagaa gaatttgaac atcgcggact taacccggtt gaagtaccag
    10141 gaattgttgt acgcaatcac ggtccattca cctggggcaa aaatccagag aatgctgttt
    10201 atcactctgt cgtactagag gaagtatcaa agatgaatcg ctttacagaa caaatcaatc
    10261 caagagttgg acctgctccc cagtacatac tagaaaaaca ctaccaacgt aaacatggac
    10321 caaatgctta ttatggtcaa aagtaagaac gatgaaggag gagaaaaaga taaatttagc
    10381 tcctcttttt acatttgatt tttattgaga gtaaagttgg agttgaagta attttgaaag
    10441 attttttaga aatagcgctt gatatatata tggtaaaata aaaagaattg ctgtgatatc
    10501 aatagatttg ggggattttt taatatggta ctggataagg caagttgtga tttacttcaa
    10561 tatttgatgg atcaagaaac gtccaaaacg attatggcga tttcgaaaga tttgaaagag
    10621 tcaagaagga aaatttatta tcacattgac aaaatcaatg ctgctctggg tgacgaggcg
    10681 cttcacatca ttagtattcc acgaattggt attcacttaa cggaagagca gagagatgct
    10741 tgttgtaaac tattatcgga agtagattcg tacgattata tcatgagtgc acatgaacgt
    10801 atgatgataa tgttactatg gataggtatt tctaaagaac gtattacgat tgaaaaattg
    10861 atagagttaa cagaggtatc taggaatact gttctcaatg atttgaatag tattcgttat
    10921 caactaactt tggaacaata tcaggtgatc ttgcaagtga gcaagtcaca gggataccac
    10981 cttcatgccc accctcttaa taaaattcag tatcttcaat cgcttctata tcatattttt
    11041 atggaagaaa atgccacttt tgtatctatt ttagaagata agatgaaaga gaggttagat
    11101 gatgagtgtt tgctttctgt tgaaatgaac caatttttta aggaacaggt tcctttagtt
    11161 gaacaagatt tagggaagaa aataaaccat catgaaataa cttttatgtt gcaggttcta
    11221 ccttatttgc tgttaagctg tcataatgtt gaacagtatc aagaaagaca tcaggatata
    11281 gagcaagaat tttctttgat aagaaaaaga atagagtatc aggtgtctaa gaagttagga
    11341 gaacggttgt ttcaaaagtt tgaaatttct ttgtcaggac ttgaagtttc tcttgtagct
    11401 gttctcctcc tctcctatcg taaagatttg gatattcatg cagaaagtga tgattttcgg
    11461 caattaaaac ttgctttaga agaatttatc tggtattttg aatcacaaat ccgaatggag
    11521 attgagaaca aggatgattt gttacgaaat ttgatgatcc actgtaaagc cttgttattt
    11581 agaaagactt acggtatttt ttctaaaaat cctctaacaa aacaaattcg atccaagtat
    11641 ggagaattat ttttagtcac tagaaaatct gcggaaattt tagaaggagc atggtttatt
    11701 cggctaacag acgatgatat tgcctatttg acgattcata ttggaggatt tttaaaatat
    11761 acaccatcgt ctcaaaaaaa tatgaaaaaa gtttatctcg tttgtgatga aggtgttgcg
    11821 gtttcgagac ttttgctgaa acaatgcaaa ctttattttc caaatgagca aattgacact
    11881 gtatttacaa cagaacaatt taagagtgtg gaagatattg cacaagttga tgtagtgatt
    11941 actactaatg atgatttgga tagcagattt ccgattttaa gggttaatcc tatccttgaa
    12001 gcagaagata ttttgaaaat gctagactat cttaaacaca atatatttcg taataagagc
    12061 aaaagtttca gtgaaaatct ttctagtctt atttcgtctt atattgtaga cagcaagttg
    12121 gctagtaagt tccaagaaga ggttcaaaca cttataaatc aagaaatagt agttcaagct
    12181 tttttggaag atatttgaag gacagtccaa tgatgaacac aaacctgtgt ttttcttggt
    12241 cttttttagt gttttgaagg gtggtatact aatctcaaag ataacaatta tatccaaagg
    12301 aggcaacata tgccaaacgt caaagaaatt acaagagagt catggatttt agccactttc
    12361 ccagagtggg gaacatggtt gaacgaagaa atcgaagaag aagtcgtacc tgaaggcaac
    12421 tttgccatgt ggtggctagg caactgtggt acttggatta agacaccagc tggtgctaac
    12481 gttgtcatgg acctttggtc aaaccgtgga aaatcaacca aaaaagtgaa agatatggtt
    12541 cgtgggcacc aaatggcaaa tatggcaggt gttcgtaagt tgcaaccaaa cttgcgtgtt
    12601 cagccaatgg ttatcgatcc atttgctatc aacgaactag actattactt agtttcacac
    12661 ttccacagtg atcatatcga cccatacaca gctgcagcaa ttctcaacaa tcctaagtta
    12721 gagcatgtta agtttatcgg tccttaccac tgtggacgaa tctgggaagg atggggtgtt
    12781 ccaaaagaac gtatcatcgt tgttaaacca ggtgacacta tcgaattaaa agatatgaag
    12841 attcatgcag tagaatcatt tgaccgtact tgcttggtaa ctctcccagt gaacggtgct
    12901 gatgagacag gcggtgaact tgctggcttg gctgttacag atgaagaaat ggctcaaaag
    12961 gctgttaact atatctttga aacaccaggt ggaaccatct atcatggtgc agattctcac
    13021 ttctcaaact attttgcaaa acatggtaaa gactttaaaa ttgatgttgc tttgaataac
    13081 tatggtgaaa atccggtagg tatccaagac aaaatgacat ctatcgacct tcttcgtatg
    13141 gcagaaaatc tgcgtaccaa agtcattatc ccagttcact atgatatctg gtctaacttc
    13201 atggcttcta ctaatgagat tctagaactt tggaaaatgc gaaaagatcg cttgcaatac
    13261 gatttccatc catttatctg ggaagttggc ggtaagtaca cttatcctca agatcaacac
    13321 ttagtagaat accatcatcc acgtggtttt gatgattgtt ttgaacaaga ctctaacatt
    13381 caatttaaag ctttgctata atataaaaat attctttgga ggttatccgt atgttgcatg
    13441 atacggataa ttttcatata atagtaaaat agaatgtgtg attcaataat cacctcaaat
    13501 agaaaggaaa ttctatgtca aatctatctg ttaatgcaat tcgttttcta ggtattgacg
    13561 ccattaataa agccaactca ggtcatccag gtgtggttat gggagcggct ccgatggctt
    13621 acagcctctt tacaaaacaa cttcatatca atccagctca accaaactgg attaaccgcg
    13681 accgctttat tctttcagca ggtcatggtt caatgctcct ttatgctctt cttcaccttt
    13741 ccggttttga agatgtcagc atggatgaga ttaagagttt ccgtcaatgg ggttcaaaaa
    13801 caccaggtca cccagaattt ggtcatacgg cagggattga tgctacgaca ggtcctctag
    13861 ggcaagggat ttcaactgct actggttttg cccaagcaga acgtttcttg gcagccaaat
    13921 ataaccgtga aggctacaat atctttgacc actatactta cgttatctgt ggagacggag
    13981 acttgatgga aggtgtctca agcgaggcag cttcatacgc aggtttgcaa aaacttgata
    14041 agttggttgt tctttatgat tcaaatgata tcaacttgga tggtgagaca aaggattcct
    14101 ttacagaaag tgttcgtgac cgttacaatg cctacggttg gcatactgcc ttggttgaaa
    14161 atggaacaga cttggaagcc atccatgctg ctatcgaaac agcaaaagct tcaggcaagc
    14221 catctttgat tgaagtgaag acggttattg gatacggttc tccaaacaaa caaggaacta
    14281 atgctgtaca cggcgcccct cttggagcag atgaaactgc atcaactcgt caagccctcg
    14341 gttgggacta cgaaccattt gaaattccag aacaagtata tgctgatttc aaagaacatg
    14401 ttgcagaccg tggcgcatca gcttatcaag cttggactaa attagttgca gattataaag
    14461 aagctcatcc agaactggct gcagaagtag aagccatcat cgacggacgt gatccagtcg
    14521 aagtgactcc agcagacttc ccagctttag aaaatggttt ttctcaagca actcgtaatt
    14581 caagtcaaga tgccttgaat gttgtggcag ctaaattacc aaccttccta ggtggatcag
    14641 ctgaccttgc tcactcaaac atgacttata tcaagacgga tggacttcaa gacgatgcca
    14701 atcgcttgaa tcgtaacatt cagtttggtg ttcgtgaatt tgcaatggga acaatcttga
    14761 acgggatggc ccttcatggt ggacttcgtg tatacggtgg aactttcttc gtcttctctg
    14821 actatgtgaa ggcagctgtc cgcttgtcag ccttacaagg acttcctgtg acttatgtct
    14881 ttacccatga ttcaatcgca gttggggaag atggtccgac tcacgaacca gttgagcatt
    14941 tagcaggtct tcgtgctatg ccaaatctaa atgttttccg tccagcagat gcgcgtgaaa
    15001 cgcaagcagc ttggtacctt gcagtgacaa gtgagaaaac accaactgcc cttgtcttga
    15061 cacgtcaaaa cttgactgtt gaagatggaa cagacttcga caaggttgct aaaggtgctt
    15121 atgttgtata tgaaaatgca gccgactttg ataccatctt gattgcgaca ggttcagagg
    15181 ttaatcttgc tgtctcagct gctaaagaat tggctagtca aggcgaaaaa atccgcgtag
    15241 tcagcatgcc atctacagat gtctttgata aacaagatgc agcttacaag gaagaaattc
    15301 ttccaaatgc agtccgccgt cgtgttgcag tcgaaatggg tgcaagtcaa aactggtaca
    15361 aatatgttgg tctcgatggt gccgttctag gtattgatac cttcggagcc tctgccccag
    15421 caccaaaagt attggcagaa tatggcttta ctgtcgaaaa tcttgtaaaa gttgttcgaa
    15481 atttgaaata atcctaaaaa tcagggcgta agctctggtt tttcttacca gaaaagtaag
    15541 gtacaatctt gtaaaagaag ctgaaatttg atatagtagt cctatgtaaa agacaaagga
    15601 gaaaacaatg aatccaaata ttactttttt aatcatgctt gtaggtatga tggccttgat
    15661 gttctttatg caacgttctc aaaagaaaca agctcaaaag cgtatggaaa gcttaaataa
    15721 actacaaaaa ggctatgaag tgattacaat cggtggactt tatggaacag tcgatgaagt
    15781 agatacggag aagggaacaa tagttcttga cgtagatgga gtttacttga cttttgaact
    15841 agctgctatc aagacagtat taccgctgaa agaaacagct tcactagaag gcgcaattga
    15901 aaaataagac gggatcacga actcccgttt tctataaaag aaaggaaatg ggatgaaaaa
    15961 attagtcttt gtctgtctgg gaaatatttg ccgtagccct atggccgagt ttgttatgaa
    16021 atcaatgaca gataactacg aaatccaaag tcgagcaact tcctcttggg aacatggcaa
    16081 tccgattcat aaggggactc agggaatttt tcaagagtat gagattcctt atgacaagaa
    16141 caagacatcg cttcagatta gtaaggaaga ttttgaagcc tttgattata ttatcggaat
    16201 ggacgcttca aatgtttccg acttacgtca gatgtgtcca gtagactgtc aagataagat
    16261 ttactcattt tcatctgaaa gtgttccaga cccttggtat acaggagatt ttgaagaaac
    16321 ctatcgacgt gttcaagagg gctgtcaagc ttggttagaa cgtttagaaa aggagagtta
    16381 aggtggaaaa tcttacgaat ttttacgaaa agtatcgtgt ttatctgact cgtccacgtt
    16441 tagagatttt gacagtagtt accattgttt tctgtgctgt actcgtcttt tttctaaata
    16501 ttccaggaaa aggtgtctta aaactcgata atggaacgat tgtttatgat ggcagtcttg
    16561 tccgcggtaa aatgaatggc caaggtacca ttaccttcca aaatggagac caatatacag
    16621 gtggcttcaa caatggagcc ttcaacggaa aaggtacctt tcaatctaaa gaaggctgga
    16681 cctacgaagg tgattttgta aatggtcagg ctgaaggaaa agggaaacta acaacagaac
    16741 aagaagtcgt ttatgaagga acttttaaac aaggcgtttt tcaacaaaaa taaagcctcc
    16801 ttatcaaagg aggtattatt agaattacaa ggtaagcgct tacctgtaaa tccctttctt
    16861 tccaaatccc tcttccaagc aagtttgtga aataaaaaat atttgaaata aatttcacaa
    16921 actttaaaga taaaacctga taagaaaaga aaatgagaaa agtttcacaa gagtttaaaa
    16981 attctttgtg aaatgtttat gaaactgttt acaaagttga aataatgcgt tataataaac
    17041 ttgtgaaaaa attaacaaag gatatcgatc cttgaaagct atggaggaaa atatggctga
    17101 taaaaaaact gtgacaccag aggaaaagaa actcgttgct gaaaaacacg tagatgagtt
    17161 ggttcaaaaa gctctagttg cccttgaaga aatgcgtaaa ttggatcaag agcaagttga
    17221 ctacatcgtt gccaaagcat cagtagcagc tttggatgcc cacggagaat tggctttaca
    17281 tgcctttgaa gaaacaggac gtggtgtatt tgaagataaa gcaactaaga acttgtttgc
    17341 ttgtgaacac gtagtaaaca acatgcgcca cactaagaca gttggcgtta tcgaagaaga
    17401 cgatgtaaca ggattgactc ttattgctga accagttggt gttgtttgtg gtattactcc
    17461 aacaacaaac ccaacatcaa cagcaatctt caaatcattg atttcattga agacacgtaa
    17521 cccaatcgtc tttgccttcc atccatcagc acaagaatca tctgctcatg cagctcgtat
    17581 cgtccgcgat gcagctatcg cagctggtgc tcctgaaaac tgtgtgcaat ggattactca
    17641 accatctatg gaagcaacaa gtgcccttat gaaccacgaa ggtgttgcga caatccttgc
    17701 aacaggtggt aatgccatgg ttaaggcggc ttattcatgt ggtaaaccag ctcttggggt
    17761 aggtgccgga aacgttccag cttatgttga aaaatcagca aacattcgtc aagcagcaca
    17821 cgatatcgtc atgtctaaat catttgataa cggtatggtc tgtgcatctg aacaagcagt
    17881 tatcattgat aaagaaattt acgatgaatt tgtagcagag ttcaaatctt accacactta
    17941 ctttgtaaac aaaaaagaaa aagctcttct tgaagagttc tgcttcggcg tcaaagcaaa
    18001 cagcaaaaac tgtgctggtg caaaattgaa cgctgacatc gttggtaaac cagcaacttg
    18061 gattgcagaa caagcaggat ttacagttcc agaaggaaca aacattcttg ctgcagaatg
    18121 taaagaagtt ggcgaaaatg agccattgac tcgtgaaaaa ttgtcaccag ttattgcagt
    18181 tttgaaatct gaaagccgtg aagatggtat tactaaggct cgtcaaatgg ttgaatttaa
    18241 cggtcttgga cactcagcag ctatccacac agctgacgaa gaattgacta aagaatttgg
    18301 taaagctgtt aaagctattc gtgttatctg taactcacct tctacttttg gtggtatcgg
    18361 ggacgtttac aatgccttct tgccatcatt gacacttgga tgtggttctt acggacgcaa
    18421 ctcagttggg gataacgtta gtgccattaa cctcttgaat atcaaaaaag tcggaagacg
    18481 gagaaataac atgcaatgga tgaaacttcc ttcaaaaaca tactttgaac gtgattcaat
    18541 tcaatacctt caaaaatgtc gtgacgttga acgtgtcatg atcgttactg accatgccat
    18601 ggtagagctt ggtttccttg atcgtatcat cgaacaactg gaccttcgtc gcaataaggt
    18661 tgtttaccaa atctttgcgg atgtagaacc ggatccagat atcacaactg taaaccgtgg
    18721 tactgagatt atgcgtgcct tcaaaccaga taccatcatc gcactcggtg gtgggtctcc
    18781 aatggatgct gccaaagtaa tgtggctctt ctacgagcaa ccagaagtgg acttccgtga
    18841 ccttgtccaa aaattcatgg atatccgtaa acgtgccttc aagttcccat tgcttggtaa
    18901 gaagactaaa ttcatcgcga ttccaactac atctggtaca ggatctgaag taacaccatt
    18961 tgccgttatc tctgataaag caaacaaccg taaataccca atcgctgact actcattgac
    19021 accaactgtg gcaatcgtag atcctgcttt ggtattgaca gttccaggat ttgttgctgc
    19081 tgatactggt atggacgtat tgactcacgc gacagaagca tacgtatcac aaatggctag
    19141 tgactacact gatggtttag cacttcaagc cattaaattg gtctttgaaa atctcgaaag
    19201 ctcagttaag aatgcagact tccactcacg tgagaaaatg cataacgctt caacaatcgc
    19261 tggtatggcc tttgccaatg ccttcctagg tatttctcac tcaatggccc ataagattgg
    19321 tgcgcaattc cacacaatcc acggtcgtac aaatgctatc ttgcttccat acgttatccg
    19381 ttacaacggt acacgtccag ctaagacagc aacatggcct aagtacaact actaccgtgc
    19441 agatgaaaaa taccaagata tcgcacgcat gcttggactt ccagcttcta ctccagaaga
    19501 aggggttgaa tcttacgcaa aagctgtcta cgaactcggt gaacgtattg ggatccaaat
    19561 gaattttaga gaccaaggaa ttgacgaaaa agaatggaaa gaacattctc gtgaattagc
    19621 cttcctggct tatgaagacc aatgttcacc agctaaccca cgtcttccaa tggtagacca
    19681 tatgcaagaa atcatcgaag atgcatacta tggctacaaa gaaagaccag gacgccgtaa
    19741 ataattgttt atcagtctag aagcaagaca aaaactcaat ttgagggaaa gatccagtaa
    19801 tttttctatg ataaaaggca tcctatcaag gtttttgaac acctgatagg atgccttttt
    19861 atgatattga ggcctttttg ccctttttga aaaactagaa tagaaacaaa atatataata
    19921 gattgaaact ggaatagtac acttctgctt ctaaaacatt gttagaattc gatttgactg
    19981 tcctgatcga tttgtcctat tcctatt
//
LOCUS       DAYYQN010000039        18111 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYYQN010000039 DAYYQN010000000
VERSION     DAYYQN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.039"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            67..5370
                     /locus_tag="ACHBRQ_001387"
     CDS             67..5370
                     /locus_tag="ACHBRQ_001387"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HFM9593039.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDTDNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIESLKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVAKTKKD"
     gene            complement(5538..7703)
                     /gene="pbp1a"
                     /locus_tag="ACHBRQ_001388"
     CDS             complement(5538..7703)
                     /gene="pbp1a"
                     /locus_tag="ACHBRQ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HFM9593040.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGSRHQSSNVSFGINQAVETNRDWGSAMKPITDYAPALEYDIYDSTAS
                     IVHDVPYNYPGTDTPLYNWDKVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKKYGASSEKMAAAYAAFANGGIYHKPMYINKIVFS
                     DGSSKEYADPGTRAMKETTAYMMTEMMKTVLAYGTGRGAYLPWLPQAGKTGTSNYTDD
                     EIENYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFYVAAKVYRSMMTY
                     LSEDNNPGDWTMPEGLYRSGEFVFKKGARSAWTAPAPQQAPTPESSSSTSESSTSQSS
                     STTPSTNNSANNNTNNQQPNTTPGQQNQNQNQNPQPAQP"
     gene            complement(7700..8296)
                     /gene="recU"
                     /locus_tag="ACHBRQ_001389"
     CDS             complement(7700..8296)
                     /gene="recU"
                     /locus_tag="ACHBRQ_001389"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9593041.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8362..8889
                     /locus_tag="ACHBRQ_001390"
     CDS             8362..8889
                     /locus_tag="ACHBRQ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9593042.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8959..9288
                     /gene="gpsB"
                     /locus_tag="ACHBRQ_001391"
     CDS             8959..9288
                     /gene="gpsB"
                     /locus_tag="ACHBRQ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9593043.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9309..9689
                     /gene="rnpB"
                     /locus_tag="ACHBRQ_001392"
     ncRNA           9309..9689
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBRQ_001392"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9774..10931
                     /locus_tag="ACHBRQ_001393"
     CDS             9774..10931
                     /locus_tag="ACHBRQ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9593044.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREMRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10944..12338
                     /gene="mapZ"
                     /locus_tag="ACHBRQ_001394"
     CDS             10944..12338
                     /gene="mapZ"
                     /locus_tag="ACHBRQ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HFM9593045.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12414..13838
                     /gene="gndA"
                     /locus_tag="ACHBRQ_001395"
     CDS             12414..13838
                     /gene="gndA"
                     /locus_tag="ACHBRQ_001395"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9593046.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13850..14539
                     /locus_tag="ACHBRQ_001396"
     CDS             13850..14539
                     /locus_tag="ACHBRQ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9593047.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14637..15653
                     /gene="cbpC"
                     /locus_tag="ACHBRQ_001397"
     CDS             14637..15653
                     /gene="cbpC"
                     /locus_tag="ACHBRQ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HFM9593048.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATDWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMATGWYQDGSTWYYLNASNGGMKTGWFQVNGKWYYAYGSGALAVNTTVDGYSVNYNG
                     DWVR"
     gene            complement(15772..17004)
                     /locus_tag="ACHBRQ_001398"
     CDS             complement(15772..17004)
                     /locus_tag="ACHBRQ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9593049.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILYQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(17047..17634)
                     /locus_tag="ACHBRQ_001399"
     CDS             complement(17047..17634)
                     /locus_tag="ACHBRQ_001399"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HFM9593050.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHIFHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
     gene            complement(17534..17923)
                     /locus_tag="ACHBRQ_001400"
     CDS             complement(17534..17923)
                     /locus_tag="ACHBRQ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HFM9593051.1"
                     /translation="MYPPELDSKILSIIKKAVTIRTTHTYEYQYSLLFGDAGYLWLLL
                     HLFSISKNQYYLQLANVTAKKLIENYDTLEEIDFALGKSGVLLPLIKYYQFTNDNTLK
                     TFIHNSIWGNLSLFPTKRYSQRKHFRL"
BASE COUNT         5606 a         3291 c         4092 g         5122 t
ORIGIN      
        1 aggaaggagt attttttaaa aagaaaatgt aaacgcttgc tcaaaaatga aaggatttag
       61 aagtttatga ataaaggatt atttgaaaaa cgttgtaaat atagtattcg gaaattttca
      121 ttaggtgttg cttctgttat gattggagct gcattctttg ggacaagtcc ggttcttgca
      181 gatagcgtgc agtctggttc cacggcgaac ttaccagctg atttagctac tgctcttgca
      241 acagcaaaag agaatgatgg gcgtgatttt gaagcgccta aggtgggaga agaccaaggt
      301 tctccagaag ttacagatgg acctaagaca gaagaagaac tattagcact tgaaaaagaa
      361 aaaccggctg aagaaaaacc aaaagaggat aaacctgcag ctgctaaacc tgaaacacct
      421 aagacggtaa cccctgaatg gcaaacggta gagaaaaaag aacaacaggg aacagtcact
      481 atccgagaag aaaaaggtgt ccgctacaac caactatcct caactgctca aaatgataac
      541 gcaggtaaac cagccctgtt tgaaaagaag ggcttgaccg ttgatgccaa tggaaatgca
      601 actgttgatt taaccttcaa agatgattct gaaaagggca aatcacgctt tggtgtcttc
      661 ttgaaattta aagataccaa taataatgtt tttgtcggtt atgacaagga tggctggttc
      721 tgggagtata aatctccaac aactagcact tggtatagag gtagtcgtgt tgctgctcct
      781 gaaacaggat caacaaaccg tctctctatc actctcaagt cagacggtca gctaaatgcc
      841 agcaataacg atgtcaatct ctttgacaca gtgactctac cagctgcggt caatgaccat
      901 cttaaaaatg agaagaagat ccttctcaag gcgggctctt atggcaatga ccgtacggtt
      961 gtcagcgtta aaacagacaa tcaagaaggc gtaaaagcgg atgatactcc tgcccagaaa
     1021 gaaacaggtc cggttgttga cgatagcaag gtgacttatg atacgatcca gtctaaggta
     1081 cttaaggcag taattgacca agccttccct cgtgtcaagg aatacacctt gaatggacat
     1141 actttgccag gacaggtgca acagttcaac caagtcttta tcaataacca ccgaatcacc
     1201 cctgaagtca cttataagaa aatcaatgag acaacagcag agtacttgat gaagattcgc
     1261 gatgatgctc acttaatcaa tgcggaaatg acagtacgct tgcaagttgt ggacaatcaa
     1321 ttgcactttg atgtgaccaa gattgtcaac cacaatcaag tcactccagg tcaaaagatt
     1381 gatgacgaaa gaaaactact ttcttctatt agtttcctcg gcaatgcttt agtctctgtt
     1441 tctagtgatc aaactggtgc taagtttgat ggggcaacca tgtcaaacaa tacgcatgtc
     1501 agcggagatg atcatatcga tgtaaccaat ccaatgaagg atttggctaa gggttacatg
     1561 tatggatttg tttctacaga taagcttgct gctggtgttt ggagtaactc tcaaaacagc
     1621 tatggtggtg gttcgaatga ctggactcgt ttgacagctt ataaagaaac agtcggaaat
     1681 gccaactatg taggaatcca cagctctgaa tggcaatggg aaaaagctta taagggcatt
     1741 gttttcccag aatacacgaa ggaacttcca agtgctaagg ttgttatcac tgaagatgcc
     1801 aatgcagaca agaacgttga ttggcaagat ggtgccattg cttatcgtag cattatgaac
     1861 aatcctcaag gttgggaaaa agttaaggat atcacagctt accgtatcgc gatgaacttt
     1921 ggttctcaag cacaaaatcc attccttatg accttggatg gtatcaagaa aatcaatctc
     1981 catacagatg gtcttgggca aggtgttctc cttaaaggat atggtagcga aggccatgac
     2041 tctggtcact tgaactatgc tgatattggt aagcgtatcg gtggtgtcga agacttcaag
     2101 accctaattg agaaggctaa gaaatatgga gctcatctag gtatccacgt taacgcttca
     2161 gaaacttatc ctgagtctaa atacttcaat gaaaaaattc tccgtaagaa tccagatgga
     2221 agttatagct atggttggaa ctggctagat caaggtatca acattgatgc tgcctatgac
     2281 ctagctcatg gtcgtttggc acgttgggaa gatttgaaga aaaaacttgg tgacggtctc
     2341 gactttatct atgtggacgt ttggggtaat ggtcaatcag gtgataacgg tgcctgggct
     2401 acccacgttc ttgctaaaga aattaacaaa caaggctggc gctttgcgat cgagtggggc
     2461 catggtggtg agtacgactc taccttccat cactgggcag ctgacttgac ctacggtggc
     2521 tacaccaata aaggtatcaa cagtgccatc acccgcttta tccgtaacca ccaaaaagat
     2581 gcttgggttg gggactacag aagttatggt ggtgcagcca actatccact gctaggtggc
     2641 tacagcatga aagactttga aggctggcaa ggaagaagtg actacaatgg ctacgtgact
     2701 aacctctttg ctcatgacgt catgactaag tacttccaac acttcactgt aagtaaatgg
     2761 gaaaatggta caccggtgac tatgaccgat aacggtagca cctataaatg gactccagaa
     2821 atgcgagtgg aattggtaga tactgacaat aataaagtag ttgtaactcg taagtcaaat
     2881 gatgtcaata gcccacaata tcgcgaacgt acagtaacgc tcaacggacg tgtgatccaa
     2941 gatggttcag cttacttgac tccttggaac tgggatgcaa atggtaagaa actttctact
     3001 gataaggaaa agatgtacta cttcaatacg caggccggtg caacaacttg gacccttcca
     3061 agcgattggg caaagagcaa ggtttacctt tacaagctaa ctgaccaagg taagacagaa
     3121 gagcaagaac taactgtaaa agatggtaaa attaccctag atcttctagc aaatcaacca
     3181 tacgttctct atcgttcaaa acaaaccaat cctgaaatgt catggagtga aggcatgcac
     3241 atctatgacc aaggatttaa cagtggtacc ttgaaacatt ggaccatttc aggcgatgct
     3301 tctaaggcag aaattgtcaa gtctcaaggg gcaaacgata tgcttcgtat tcaaggaaac
     3361 aaagaaaaag ttagtctcac tcagaaatta actggcttga aaccaaatac caagtatgcc
     3421 gtttatgtcg gtgtcgataa ccgtagtaat gccaaggcaa gtatcactgt gaatactggt
     3481 gaaaaagaag tgactactta taccaataag tctctcgccc tcaactatgt aaaagcctac
     3541 gcccacaata cacgtcgtga caatgctaca gttgacgata caagttactt ccaaaacatg
     3601 tacgccttct ttacaactgg atcggacgtc tcaaatgtta ctctgacatt gagtcgtgaa
     3661 gctggtgatc aagcaactta ctttgatgaa attcgtacct ttgaaaacaa ttcaagcatg
     3721 tacggagaca agcatgatac aggtaaaggc accttcaagc aagactttga aaatgttgct
     3781 cagggtatct tcccatttgt agtgggtggt gttgaaggtg ttgaagataa ccgcactcac
     3841 ttgtctgaaa aacacgatcc atatacacaa cgtggttgga atggtaagaa agtcgatgat
     3901 gttatcgaag gaaattggtc actcaagaca aatggactag tgagccgtcg taacttggtt
     3961 taccaaacta ttccgcaaaa cttccgtttt gaagcaggta agacctaccg tgtaaccttt
     4021 gaatacgaag caggttcaga caatacctat gcttttgtag tcggtaaggg agaattccag
     4081 tcaggtcgtc gtggcactca agcaagcaac ttggaaatgc atgaattgcc aaatacttgg
     4141 acagattcta agaaagccaa gaaggcaacc ttccttgtga caggtgcaga aacaggggat
     4201 acttgggtag gtatctactc aactggcaat gcaagtaata ctcgtggtga ttctggtgga
     4261 aatgccaact tccgtggtta taacgacttc atgatggata atcttcaaat cgaagaaatt
     4321 accctaacag gtaagatgtt gacagaaaat gctctgaaga actacttgcc aacggttgcc
     4381 atgactaact acaccaaaga gtctatggat gctttgaaag aggcggtctt taacctcagt
     4441 caggccgatg atgatatcag tgtggaagaa gcgcgtgcag agattgccaa gattgaatcc
     4501 ttgaagaatg ctttggttca gaagaaaacg gctttggtag cagaagactt tgcaagtctt
     4561 acagctcctg ctcaggctca agaaggtctt gcaaatgcct ttgatggaaa cttatctagt
     4621 ttatggcata catcatgggg cggtggagat gtaggcaagc ctgcaaccat ggtcttgaaa
     4681 gaaccaactg aaatcactgg acttcgttac gttccacgtg gatcgggttc aaatggtaac
     4741 ttgcgtgatg tgaaacttgt tgtgacagat gagtctggca aggagcatac ctttactgca
     4801 actgattggc cagataacaa taagccaaaa gacattgatt ttggtaagac aattaaggct
     4861 aagaaaattg tccttacagg tactaagact tacggagatg gtggcgataa ataccaatct
     4921 gcagcggaac ttatctttac tcgtccacag gtagcagaaa cacctcttga cttgtcaggc
     4981 tatgaagcag ctttggctaa ggctcagaaa ttaacagaca aagacaatca agaggaagta
     5041 gctagcgttc aggcaagcat gaaatatgcg acggataacc atctcttgac ggaaagaatg
     5101 gtggaatact ttgcagatta tctcaaccaa ttaaaagatt ctgctacgaa accagatgct
     5161 ccaactgtag agaaacctga gtttaaactt agctctttag cttccgagca aggtaagacg
     5221 ccagattata agcaagaaat agctagacca gaaacacctg aacaaatctt gccagcaaca
     5281 ggtgagagtc aatctgacac agccctcttc ctagcaggag ttagcctagc cctatctgct
     5341 ctctttgtag caaaaacaaa gaaagactag tatttagtaa aacctcttaa caagattacg
     5401 aaatccgttt cctatctttc ccaatgaggt ttaaagtaca gaaaaagcct gagaagacat
     5461 cttctcaggc ttttgttaag catataaatg caatagtgct atgaaaaaat cacccagaaa
     5521 aatctgggtg ataaatgtta tggttgtgct ggttgaggat tctgattttg gttctggttt
     5581 tgttgaccag gcgttgtatt tggttgctgg ttattggtat tattgtttgc actattattc
     5641 gtgcttggag tagttgagct tgactgtgaa gttgaacttt ctgatgtcga gctcgaactt
     5701 tctggtgttg gggcctgttg cggagcagga gcagtccatg cagaacgagc acctttttta
     5761 aagacgaact caccacttcg atagagacct tctggcatag tccagtcgcc agggttgtta
     5821 tcctcagaca gataagtcat cattgaacgg taaaccttag ctgcaacata gaagccatca
     5881 ccaacgatag gagtcaggcg gtttgagtaa cctgtccata ccgccattga atatttgcga
     5941 gtataaccaa caaacatttc gtctggagct acataaccag tatttttgat gtagttttca
     6001 atttcatcat ctgtatagtt tgatgtacca gtcttaccag cttgaggtag ccaagggaga
     6061 taagcaccac gacccgttcc gtatgccaag acagtcttca tcatttctgt catcatatag
     6121 gcggtcgtct ctttcatggc acgagtacca ggatcagcgt attcttttga gctaccatcg
     6181 ctaaagacga ttttgttgat atacattggt ttatggtaga taccaccgtt agcaaaagca
     6241 gcgtaagcag cagccatttt ctcactactt gctccgtact ttttgtttga ctcagtcgta
     6301 ttacttgaaa tggcgtttgc ataatgcatg cttggatagt cgataccaag accattaaga
     6361 aaggttttag ctctatctag accgacctta ttcaaagtct caacggctgt gacattacgt
     6421 gactgttgaa gtgcatactg gattgtaata tttccaaagt agactttatc ccagttgtag
     6481 aggggagtat cagtacctgg atagttataa ggaacatcat gtacaatcga agcagttgag
     6541 tcgtagatgt catactctaa ggcaggagca tagtctgtga ttggcttcat ggcagaaccc
     6601 cagtcacggt tggtttcaac agcttggttg attccgaagg aaacattgct tgattggtga
     6661 cgagaaccta actgagcaat aactttcccg ttagaaacat caacaatggt agaagcgact
     6721 tgcaattcat cgtctggata ggcaacgtat tcgtctgtat tgtaaatatc ccacagatgt
     6781 ttttgagctt cttggtctac atttgtgtag acatccatcc cagttgtgag caggttatag
     6841 cctgtttctt cttcaacttg attgatgact tccttgaggt aattatccat gtaagcaggg
     6901 taattacttg ctgatttgag actttgtagt ccatcagtaa ttggtgtatt gactgctttc
     6961 tcatactgtt cagcagagat gtagccttga tttttcattt cagataagac caagtttcgg
     7021 cggtcttggg ctgcttctgg atgtgaatag gggtcatatt ggtttggtgc ctgaggcatt
     7081 ccagccagca aggctaactg aggtaaactt aaattattga ggtctttacc atagtagttt
     7141 tgagctgctg tctgcattcc atagttccca ttagacatgt agaccttatt tatatagtag
     7201 gtcaagattt cttgcttggt tgctttttgt tctaactgaa tcgctaacca agcttcctga
     7261 gccttacgag aaatagtctg gtcggaagtc gaagttgaaa agtaagtcaa cttaatcaac
     7321 tgttgagtga gagttgatcc accttggagg gaattgcttt gcagattgcg caagaaagct
     7381 cccaggatac ggatggtatc aatccccctg tggtcgaaga agcgatggtc ttcgatagaa
     7441 acgattgcct taaccaaatc tgtgggaata tcattagctt gggcattgac gcggcgttca
     7501 gaacccaagt cagcaatgag ttgattttta ttgtcgtaga ttttactaga agttgttgca
     7561 actagtttac tctcggatag gctaggagcc ttgctaacat agtagaaaaa aactcctccg
     7621 cctaagacaa tggctgcgat aaccaagctt aagaagctaa tgctcagata cttgattagg
     7681 cgcagaatcg ttggtttgtt catcttgttt taccacctaa taaatgttct ttgataacat
     7741 tgagataagg aatttgaggg aaggcaccag ccttgatttc atatccatat tctcgaatat
     7801 attcaagtgg cattgatttt tgtcccttat cttgatgata gaagcgaatc aaatcgaatg
     7861 ccggcaataa gtaggtttct tgctgagaag aaaagtgaag aaggacaaag cagattcctt
     7921 gttgggcaag gacttgttcc atatgctgaa tctgatgtgg atgaaaattt ttcatcggaa
     7981 tcgcacgttt ttgttttgtt tccttgactt caaagtcgat gtaatatcca ttataaacgc
     8041 cagaatagtc cgtcgttgaa gcttgtcgaa aataggcttc aacaatcttg gcacgacttc
     8101 gttgtggata gtccacttgt acgatttgaa taggagttgg tttcttatgt ataacagcca
     8161 agccttgaga caaatagtag tcgttggtag cattgatcat cttttcaaaa gacattcctc
     8221 gatttgcgaa atttttgggt tgagaaagag atgtttgtct tttttgtgat gaaactttat
     8281 gtggatagtt gaccataatt ctccttattg gtacaataac atcactttat tataccataa
     8341 tttgcacaga aagggttaaa aatggctaca gctttggttt taggctattc tgcttttgat
     8401 ttgggtttat tttctgataa ggatcctcgt cttaaattga ttaaaaaagc gatacgtaaa
     8461 gatttagagg ctatggcagc agatggggtg tcttggcttg tgtttacagg gagtttgggg
     8521 tttgaatatt gggttttaga ggttgctcag gagatgaaaa ccgaatacgg tttccagttg
     8581 gccaccattt ttgcttttga aacccatggg gaaaattgga atgaaggcaa tcagatgaaa
     8641 ctgagtcgct ttaagcaggt agactttgtc aaatatgcct atcctcgcta tgaacacaag
     8701 gggcagttaa gagattacca gcagtttttg ctggaaaaca cgactagttc ctatcttttt
     8761 tatgatgaag aaaatgaaac aaaactagct tatttttacc aaaagatgaa aaatcaagag
     8821 gactatttta taaagagatt aacatttgat caattaaatg aacttgctga aaatttttcc
     8881 gaaaattgaa gctttgacct tgatttttgt ttgccttttt ttatataata atactagcaa
     8941 gcgagaatgg agagagacat ggcaagtatt attttttcag cgaaagatat ttttgaacaa
     9001 gagtttggac gtgaagtccg tggctataat aaagtagaag ttgacgagtt tttagacgat
     9061 gtcatcaagg actatgaaac ctatgctgcc ttggtcaagt cacttcgtca ggaaattgcg
     9121 gatttgaagg aagaattaac tcgtaaaccg aaaccttcac cagttcaagc agaacccctt
     9181 gaagcggcaa ttacaagttc tatgacgaat tttgatattt tgaaacgcct gaatagattg
     9241 gaaaaagaag tttttggtaa acaaatttta gataactcag atttttaagt agttatttga
     9301 gatgtgcaat ttttggataa tcgcgtgagg agaattgctt ctcatgagga aagtccatgc
     9361 tagcacaggc tgtgatgcct gtagtgtttg tgctaggcga aaccataagc ctagggacga
     9421 gaaatcgtta cggcagttga aatggctaag tccttggata ggccagagta ggcttgaaag
     9481 tgccacagtg acgaagtctt tctggaaaca gagagagtgg aacgcggtaa acccctcaag
     9541 ctagcaaccc aaattttggt cggggcatgg agtacgcgga aacgaacgta gtattctgac
     9601 tgctatcagc tagagctgtt agtggtagac agatgattat cgaaggaagt ggtcctagtc
     9661 acttctggaa caaaacatgg cttatagaaa attgcatata ggttggggct gagaaatttt
     9721 ctcaacctca ttttttaaag tggacatata gaaaggtctt gcaagactgt aacatgaaaa
     9781 aagaatttaa tttaattgca actgtggcag cagggcttga ggctgtcgtt ggtcgtgaaa
     9841 tgcgagagtt gggctacgat tgtcaggttg aaaatggacg tgttcgtttt caaggagacg
     9901 tgagagctat tatcgaaacc aacctttggc ttcgggcagc agatcgtatc aaaattatcg
     9961 taggaacgtt cccagctaag acttttgaag agctatttca gggagttttc gctttagatt
    10021 gggaaaatta tttaccactt ggagctcggt tcccgatttc aaaagctaaa tgtgttaagt
    10081 ccaaacttca caatgagccc agtgttcagg ctatttctaa gaaagctgtt gtcaagaaat
    10141 tgcagaaaca ctatgctcgc ccagaagggg ttcctctgat ggagaatggt ccagagttta
    10201 agattgaggt ctctattctc aaagatgtgg caactgtcat gattgatacg accgggtcta
    10261 gcctctttaa acgtggttat cgtaccgaaa aaggtggcgc tcctatcaag gaaaatatgg
    10321 cagcagccat tttacaactt tctaactggt atccagacaa gcctttgatt gatccgacct
    10381 gtggttcggg gactttctgt attgaggcag ttatgattgc tagaaagatg gcgccaggtc
    10441 ttcgtcgttc ttttgcattt gaggaatgga actggatcag cgatcgcttg attcaagaag
    10501 tgcgcacaga agcggctaaa aaagtagacc gtgagcttga gctggatatc atgggctgtg
    10561 atattgatgc tcgcatggtg gaaattgcta aggccaatgc tcaggtagct ggtgttgcag
    10621 gagacattac ttttaagcag atgcgcgtgc aggatttatg ttccgataaa atcaatggag
    10681 taatcatttc caatccgcct tatggtgaac gtttgtcaga tgatgcaggg gtgaccaagc
    10741 tctatgctga gatggggcaa gtatttgcac cgctgaaaac ttggagcaaa tttatcctga
    10801 ctagtgatga agcttttgaa agcaagtatg gtagccaagc agataagaag cgtaagttat
    10861 acaacggaac cttgaaagtg gatctatatc aatattttgg tcagcgtgtc aaacggcaag
    10921 aggtaaaata gaaagggata ctcatgagta aaaaaagacg aaatcgtcat aaaaaagaag
    10981 gtcaagaacc gcaatttgat tttgatgaag caaaagagct aacagttggt caagctattc
    11041 gtaaaaatga agaagtggaa gcaggagtct tgcctgagga ttccattttg gacaagtatg
    11101 ttaagcaaca cagagatgaa attgaggcgg ataagtttgc gactcgtcaa tacaaaaaag
    11161 aggagttcgt tgaaactcag agtctggatg atttaattca agagatgcgt gaggctgtag
    11221 agaagtcaga agcttcttcg gaggaagttc catcttctga agacatctta ctacccttgc
    11281 ctctggacga tgaggagcaa ggcttggatc ctctattgct agatgatgaa aatccaacag
    11341 aaatgactga agaagtggaa gaggagcaaa acctttctcg tctggatcaa gaggactcag
    11401 aaaagaaaag taaaaaaggc tttattttga ccgttttggc gcttgtatca gtaattattt
    11461 gtgtcagtgc ttattatgtc taccgtcaag tggctcgttc gactaaggaa attgaaactt
    11521 ctcaatcaac tacagccaat caatcggatg tggatgattt taatacactt tatgacgcct
    11581 tttacacaga tagcaataaa acggctttga aaaatagcca gtttgataaa ctgagtcaac
    11641 tcaagacttt acttgataag ctggaaggta gtcgtgaaca tacgcttgcc aaatctaaat
    11701 atgatagtct agtaacgcaa attaaggcca ttcaagatgt caatgcacaa tttgaaaaac
    11761 cagctattgt ggatggtgtg ttggatacca atgccaaagc caaatcggat gctaaattta
    11821 cagatattaa aactgggaat acggagcttg ataaagtgct agataaggct atcagtcttg
    11881 gtaagagcca gcaaacaagt acttctagct caagttcaag tcaaactagc agctcaagct
    11941 ctagtcaagc aagctcaaat acgactagtg agccaaaacc aagtagttca aatgagacta
    12001 gaagtagtcg cagtgaagtc aatatgggtc tctcgagtgc aggggttgct gttcaaagaa
    12061 gtgccagtcg tgttgcctat aatcagtctg ctattgatga tagtaataac tctgcctggg
    12121 attttgcgga tggtgtcttg gaacaaattc tagcaacttc acgttcacgt ggctatatca
    12181 ctggagacca atatatcctt gaacgtgtca atatcgttaa cggcaatggt tattacaacc
    12241 tctacaagcc agatggaacc tatctcttta cccttaactg taagacaggc tactttgtcg
    12301 gaaatggcgc tggtcatgcg gatgacttgg attactaagc agtcgttaca aaattctttc
    12361 ttttcaaaag taaaaatgat aaaataaaac aaatgaaaca agaggagtgt caaatgacaa
    12421 aagctaactt tggtgtcgta ggtatggccg taatgggtcg taaccttgcc cttaatattg
    12481 aatcacgtgg ttacacagtt gctatctaca accgtagtaa agaaaaaacg gaagatgtga
    12541 ttgcttgcca tcctgaaaag aattttgtac caagctatga cgttgaaagt tttgtaaact
    12601 caatcgaaaa acctcgtcgt atcatgctga tggttcaagc tggacctggt acagatgcta
    12661 ctatccaagc ccttcttcca caccttgaca aaggtgatat cttgattgac ggaggaaaca
    12721 ctttctacaa agataccatc cgtcgtaatg aagaattggc aaactcaggt atcaacttta
    12781 tcggtactgg agtttctggt ggtgaaaaag gtgcccttga aggtccttct atcatgcctg
    12841 gtggacaaaa agaggcctac gaattggttt cggatgttct tgaagaaatc tcagctaaag
    12901 caccagaaga tggcaagcca tgtgtgactt atatcggtcc tgatggagct ggtcactatg
    12961 tgaaaatggt tcacaacggt atcgagtacg gtgatatgca attgatcgca gaaagttatg
    13021 acttgatgca acacttgcta ggtctttctg cagaggatat ggctgaaatc tttactgagt
    13081 ggaacaaggg tgaattggat agctacttga tcgaaatcac agctgatatc ttgagccgta
    13141 aagacgatga aggccaagat ggaccaatcg tagactacat ccttgatgct gcaggtaaca
    13201 agggaactgg taaatggact agccaatcat ctcttgacct tggtgtacca ttgtcactga
    13261 ttactgagtc agtgtttgca cgctacattt caacttacaa agaagaacgt gtatatgcta
    13321 gcaaggtgct tccaaaacca gctgccttca actttgaagg agacaaggct gaattgattg
    13381 aaaagatccg tcaagccctt tacttctcaa aaatcatttc atacgcacaa ggatttgctc
    13441 aattgcgtgt agcctctaaa gaaaacaact ggaacttgcc atttgcagat atcgcatcta
    13501 tctggcgtga tggctgtatc atccgttctc gtttcttgca aaagattaca gatgcttaca
    13561 accgcgatgc agatcttgcc aaccttcttt tggacgagta cttcttggat gttactgcta
    13621 agtaccaaca agcagtacgt gatatcgtag ctcttgcggt tcaagcaggt gtgccagtgc
    13681 caactttctc agcagctatt acttactttg atagctaccg ttcagctgac cttccagcta
    13741 acttgatcca agcacaacgt gactactttg gtgctcacac ttaccaacgt aaagacaaag
    13801 aaggaacctt ccactactct tggtatgacg aaaaataagt aggtcagcca tggggaaacg
    13861 gattttatta cttgagaaag aacgaaatct agctcatttt ttaagtttgg aactccagaa
    13921 agagcagtat cgggttgatc tggtagagga ggggcaaaaa gccctctcca tggctcttca
    13981 gacagactat gatttgattt tattgaacgt taatctggga gatatgatgg ctcaggattt
    14041 tgcagaaaaa ttgagccgaa ctaaacctgc ctcagtcatc atgattttag atcattggga
    14101 agacttgcaa gaagagctgg aagttgttca gcgttttgca gtttcataca tctataagcc
    14161 agtccttatc gaaaatctgg tagcgcgtat ttcggcgatc ttccgaggtc gggacttcat
    14221 tgatcaacac tgcagtctga tgaaagttcc aaggacctac cgcaatctta ggatagatgt
    14281 tgaacatcac acggtttatc gtggtgaaga gatgattgct ctgacacgcc gtgagtatga
    14341 ccttttggcg acacttatgg gaagcaagaa agtattgact cgtgagcaat tgttggaaag
    14401 tgtttggaag tatgaaagtg cgaccgagac aaatatcgta gatgtctata tccgctatct
    14461 acggagcaag cttgatgtta aaggacaaaa aagctacatt aaaactgtgc gtggtgttgg
    14521 atataccatg caagaataga aaagcagttg cagttgtgta actgcttttt tgaggagttt
    14581 ttatatattg acatgcagtc gggtctttgc tacaatcgtt ttaggaggat atatagatga
    14641 aacttttgaa aaaaatgatg caagttctac tagcagtctt tttctttggt ttgctagcta
    14701 caaatacggt atttgcgaat accacaggtg gccgatttgt tgataaggat aatagaaaat
    14761 attatgtaaa agatgatcat aaagcaatct attggcataa aatagacggt aaaacttact
    14821 attttggtga tattggagag atggttgtcg gttggcaata cttagaaatt cctggaacag
    14881 gttatcgtga taatttattc gataaccaac cagttaatga aattggcctt caggagaagt
    14941 ggtactattt tggacaagat ggtgctttgc tagaacaaac agataaacaa gtactagagg
    15001 caaaaacgtc tgaaaataca ggaaaagtat acggtgaaca atatcctcta tctgctgaaa
    15061 agagaactta ttattttgat aataattatg ctgtaaagac aggctggatt tatgaagacg
    15121 gcaattggta ttatttaaat aagctaggaa attttggcga tgattcttac aatccactac
    15181 caattggtga agttgctaag ggttggactc aagattttca tgttactatt gacattgata
    15241 gaagcaaacc tgctccatgg tactacctag atgcttcagg taagatgctt acagattggc
    15301 aaaaagtaaa cggaaaatgg tattattttg gctcctctgg ttctatggca acagattgga
    15361 aatatgtacg aggcaaatgg tattacttag ataataaaaa tggtgatatg aaaacaggat
    15421 ggcaatacct tggtaacaag tggtactacc tccgttcatc aggagcaatg gcaactggtt
    15481 ggtatcaaga tggttcaact tggtactacc taaatgcaag taatggaggt atgaagacag
    15541 gttggttcca ggtcaatggc aaatggtatt atgcctatgg ttcaggtgct ttggcagtga
    15601 atacgaccgt agacggctat tctgtcaatt ataatggcga ctgggttcgg taatgacagg
    15661 gtgcttataa ttatattaga acactcgctt aatcaaagaa gtgactttgt tgtacttgac
    15721 ctgacgattg gggagattaa aaagcatgtg aaatctacac atgctttttt gttagttatg
    15781 agtgttgaac ttgactaacc attgacaaaa gataagagca atcaaaataa gatacaaaaa
    15841 tatcaatgct aactgcaccg aaacgcccga tgctatggat agggctaata gggagagcag
    15901 ggacggggcg agaatgtcca ctgtgctcag agcagagccc accgtcccca tcttgtcact
    15961 ggggatatag gcaaagacag ctgcttgtag gcgagggctg agaatgccta taatcgtaga
    16021 actgatgaaa ctggtcatga aaattacgat ataggcttga taaaggatac tagttattag
    16081 agccaatagg gaaatctcac agaaaaccaa gaggtgcgtg aaacggatat ttttaaatag
    16141 attactgctg gtcatattgc caaggatagc tccacaagac atgatgatgg agagcagggc
    16201 gaggagttgc ccaatgctta gttgtgctag tgtttgttgg atgaagcgga gggcaatcag
    16261 ccgaggggac acatccaaga tggcaatcat actggtcgtc agaaacagta gtatgacggt
    16321 atcttttaac ctcaggagca cagccagcga ttgtttaaga tgttgaaaat atttcttaaa
    16381 actcagtgct gtatgtgaca tttcaatcct tttttcaacc tcatacaagg tatgtctaat
    16441 aacatacagg cccaaaaagg caatgacaaa agtcagagtg ttgataaggg aaatagtttg
    16501 aatacttata acattgataa ggaatgcgcc agccagattg gcgacaatgc ggacaaccct
    16561 catcagagac tgcctaaacc ccatggcatc atgcaggtcg tccttaatta cactgatgta
    16621 gagggcgttc atcatgtagg cagacaggta gctcaaggtg tcagagatga ggttgatgat
    16681 gacaatcata atgactgaaa ctaatgaaaa gggcgacaag acgagaaata ctgtcaaaat
    16741 agcgtagagc aggattttga ttttggcaat ccagagtgcg tgtttgactc tattttcttg
    16801 aaaatctcct agaatcccga gaaagagttg cgatagtagg ggaaccatct ctgaaatgga
    16861 aatcagtaaa atcgctagcg gagcgaaatt gtactgagcc acataattga taatggcgag
    16921 gtagaaaata ctgtcgccga atgatgaaaa acagctgttt agcagcaact gtctatatat
    16981 gttgtttgtc caaaataatt tcatactttt ctccttatgt ctgcacatcg aatgggaatt
    17041 tattatttaa tagacaccaa tataccccat gcttcctatt ccgaagtcaa acaaatctgc
    17101 tttaccacta tctccttgaa acatcagtaa accgtgatca tctcgttcag aacatgctaa
    17161 aattacctgt tggatttttt tcatcagtaa tttgttttga ttgtagacag tggtttgtag
    17221 taagctagtt attccgtggc aatatcctgt catcattttt agatgatggt taaaaacaaa
    17281 ttcttgatat ttactaataa tatctttgtt tccgtcacaa tcgtacatac aaagatataa
    17341 gattattccg gaaattcctt tgcaccaaga aagttgtaaa tttcctaaat tttcagtatt
    17401 agaagtaact ttttctaata atttttttaa ttcagtatgg aatatatgga gatcattata
    17461 gaacatagaa ggttctaaga ctttagaata ggcaaataaa gcatatgcaa tttcacaata
    17521 tccatgagca aagctatagt ctaaaatgct ttctttggct gtatctcttt gtaggaaata
    17581 atgataaatt tccccatata ctattgtgga tgaaagtttt aagagtattg tcattggtaa
    17641 attgatagta ttttattaat ggtaatagga caccagattt tcccaatgca aagtctattt
    17701 cctctagagt atcataattc tctattaatt tcttagcggt gacgtttgct aattgtagat
    17761 agtattgatt tttactgata gaaaataaat gtaggagtaa ccatagatag cctgcatcac
    17821 caaatagcag tgagtattga tattcatatg tgtgtgtcgt tctaattgtc actgccttct
    17881 tgatgataga gagtatttta gaatccagtt ctggggggta caatttattt agaggaaaca
    17941 gtagtccagc tattccgtgt tggaatgata aattacttac gaaatttcca aattctgttg
    18001 aagaagtgat ccttccttca gaatttacag ttttatctag taaaaatcgt agaacactat
    18061 tacattctgt tttgtaatca tatttatgtt cctctatttt tttaattttg a
//
LOCUS       DAYYQN010000040        17538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYYQN010000040 DAYYQN010000000
VERSION     DAYYQN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.040"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(71..268)
                     /gene="blpI"
                     /locus_tag="ACHBRQ_001401"
     CDS             complement(71..268)
                     /gene="blpI"
                     /locus_tag="ACHBRQ_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HFM9593052.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            550..1137
                     /locus_tag="ACHBRQ_001402"
     CDS             550..1137
                     /locus_tag="ACHBRQ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFM9593053.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1064..2707
                     /locus_tag="ACHBRQ_001403"
     CDS             1064..2707
                     /locus_tag="ACHBRQ_001403"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM9593054.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLVQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2718..4079
                     /locus_tag="ACHBRQ_001404"
     CDS             2718..4079
                     /locus_tag="ACHBRQ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HFM9593055.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4136..4264
                     /gene="blpC"
                     /locus_tag="ACHBRQ_001405"
     CDS             4136..4264
                     /gene="blpC"
                     /locus_tag="ACHBRQ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HFM9593056.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4335..5675)
                     /locus_tag="ACHBRQ_001406"
     CDS             complement(4335..5675)
                     /locus_tag="ACHBRQ_001406"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9593057.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5689..6426)
                     /locus_tag="ACHBRQ_001407"
     CDS             complement(5689..6426)
                     /locus_tag="ACHBRQ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9593058.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6431..6769)
                     /locus_tag="ACHBRQ_001408"
     CDS             complement(6431..6769)
                     /locus_tag="ACHBRQ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFM9593059.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7045..7374
                     /locus_tag="ACHBRQ_001409"
     CDS             7045..7374
                     /locus_tag="ACHBRQ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HFM9593060.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7542..8591)
                     /locus_tag="ACHBRQ_001410"
     CDS             complement(7542..8591)
                     /locus_tag="ACHBRQ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593061.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8588..9319)
                     /locus_tag="ACHBRQ_001411"
     CDS             complement(8588..9319)
                     /locus_tag="ACHBRQ_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9593062.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9387..9797
                     /locus_tag="ACHBRQ_001412"
     CDS             9387..9797
                     /locus_tag="ACHBRQ_001412"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9593063.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9807..10094
                     /locus_tag="ACHBRQ_001413"
     CDS             9807..10094
                     /locus_tag="ACHBRQ_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593064.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10256..10450
                     /locus_tag="ACHBRQ_001414"
     CDS             10256..10450
                     /locus_tag="ACHBRQ_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593065.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(10784..11920)
                     /gene="dnaJ"
                     /locus_tag="ACHBRQ_001415"
     CDS             complement(10784..11920)
                     /gene="dnaJ"
                     /locus_tag="ACHBRQ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9593066.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12322..12678)
                     /locus_tag="ACHBRQ_001416"
     CDS             complement(12322..12678)
                     /locus_tag="ACHBRQ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HFM9593067.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12680..14503)
                     /gene="dnaK"
                     /locus_tag="ACHBRQ_001417"
     CDS             complement(12680..14503)
                     /gene="dnaK"
                     /locus_tag="ACHBRQ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9593068.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14983..15507)
                     /gene="grpE"
                     /locus_tag="ACHBRQ_001418"
     CDS             complement(14983..15507)
                     /gene="grpE"
                     /locus_tag="ACHBRQ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9593069.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15534..16568)
                     /gene="hrcA"
                     /locus_tag="ACHBRQ_001419"
     CDS             complement(15534..16568)
                     /gene="hrcA"
                     /locus_tag="ACHBRQ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9593070.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16732..17241)
                     /locus_tag="ACHBRQ_001420"
     CDS             complement(16732..17241)
                     /locus_tag="ACHBRQ_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFM9593071.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         5436 a         3798 c         3130 g         5174 t
ORIGIN      
        1 tattttgtaa gtaagtaaat aagacagtaa attaatagcg ataataatac tatatttaag
       61 aatcataatc ttacaaagag gacataattc ctgaacctac acaaataagt gttgctgctc
      121 ccccagttat cggaccagtc gcagcagcta atagtactgc tccaatacaa ccaccgattg
      181 cagatcctaa attgcctctt cctccactaa ctatttcgag ttcttcatta tccataacag
      241 aaaattgttc catcattttt gtattcatga caaatactcc ttttttcttt ttttattttt
      301 gtcttgttgt aactttgata agtttagtat atcatcgttt tttaaaattt ttcatccaga
      361 tcttgaattg tcatcgaaac gtcttgaaat aggtttttta tttcaagcca cctctaaatg
      421 tttaaaaaaa ataatttcta atcacttttt taccattcag gaagttttaa tgactattca
      481 agatttcata aaatatgaac ttagttttat gacataatag acctatccac tatatgaaag
      541 gaattgccaa tgacttctta taaacgtaca tttgttcctc aaatagatgc gagagactgt
      601 ggtgtcgctg ccttagcctc gattgctaaa ttctatggtt cagatttttc tctagctcac
      661 ttgagagaac ttgcaaagac caataaagaa gggacgactg ctcttggcat tgtaaaagcc
      721 gctgatgaaa tgggctttga aacaagacct gttcaagcag ataaaacgct ctttgacatg
      781 agtgatgtcc cctatccatt tatcgttcac gttaacaaag aaggaaaact ccaacattac
      841 tatgttgtct atcaaacaaa gaaagactat ctgattattg gtgatcctga cccttctgta
      901 aaaatcacta aaatgtcaaa agaacgcttt ttctatgaat ggactggagt agctattttt
      961 ctagctacca aacccagcta tcaaccccat aaagataaaa agaatggtct actaagcaag
     1021 cttccttcct ctgattttca aacaaaaatc tctcattgct tacattgttc tctcaagctt
     1081 attggtcact attatcaata taggtggttc ttactatctc caaggaatct tggatgaata
     1141 cattccaaat cagatgaaat caactttagg aatcatctca gttggtctgg ttatcaccta
     1201 tatcctccaa caagtcatga gcttctccag agattatctc ctaaccgttc tgagtcagag
     1261 attaagtatt gatgtgattt tatcctatat tcgccatatt tttgaacttc ccatgtcttt
     1321 ctttgcgaca cgtcgtacag gagaaatcat ttcacgattc acagatgcta actctattat
     1381 agatgccttg gcttctacca ttctttctct ttttctggat gtttctattc tgattcttgt
     1441 aggaggcgtc ttactggtac aaaaccctaa tctcttcctt ctttctctta tttccattcc
     1501 tatatacatg ttcatcatct tttcttttat gaaacctttc gaaaaaatga accatgatgt
     1561 catgcaaagt aattctatgg ttagctctgc cattatcgaa gatatcaacg ggattgaaac
     1621 tataaagtcg ctcacgagtg aagaaaatcg ctatcaaaat atagacagcg aatttgtaga
     1681 ttatttggaa aaatccttta agctcagtaa atattctatt ttacaaacga gtttaaagca
     1741 gggaacaaaa ttagttctga atatccttat cctatggttt ggcgctcaat tagtcatgtc
     1801 gagtaaaatt tctatcggtc agctgattac ctttaacaca cttttttctt actttacaac
     1861 tcctatggaa aatattatca acctccaaac caaactccaa tctgcgaagg tcgctaataa
     1921 ccgtttgaac gaagtctatc tagtcgaatc tgaatttcaa gttcaagaaa accctgtcca
     1981 ttcacatttt ttgatgggcg atattgaatt tgatgacctt tcttataagt atggttttgg
     2041 acgagatacc ttaacagata ttaatctcac gattaaacaa ggagataagg ttagcctagt
     2101 tggagttagt ggttctggta aaacaacttt agccaaaatg attgtcaatt tctttgaacc
     2161 ctacaaagga catatttcta tcaatcatca ggatattaaa aacattgata aaaaagtctt
     2221 gcgccgtcat attaattacc taccacaaca agcctatatc tttaatggct ctatcttgga
     2281 aaacttaact ttgggcggta atcatatgat tagccaagaa gatattctaa gagcttgtga
     2341 attagctgaa attcgtcaag acattgaaag aatgcctatg ggctatcaaa ctcagctctc
     2401 tgatggagct ggtctatcag gaggacaaaa gcaacgaata gccctcgctc gtgctctttt
     2461 aactaaagct cctgttttaa tactagacga agctactagc ggtcttgatg tcttgactga
     2521 gaaaaaggtt atagataatc ttatgtctct aactgataaa accattctct ttgtagccca
     2581 tcgtctcagt atagccgaac gaaccaaccg tgtcattgtt cttgaccagg ggaaaatcat
     2641 tgaagttggt agtcaccaag agttaatgca ggcgcaaggt ttctaccatc atctattcaa
     2701 taaataagga gaatgtcatg aatcctaatc tttttagaag cgtcgagttt tatcagagac
     2761 gttaccataa ctatgcgaca gtgttcatta tacctctttc attactattt actttcatct
     2821 tgattttctc ccttgttgcc acaaaagaaa ttactgttac ttcccaagga gaaatcgccc
     2881 ctacaagtgt cattgcctcc attcagtcaa ccagtgataa tcctatccta gctaatcatt
     2941 tagtggcaaa tcaagtagtt gaaaaagggg acttactcat caaatactct gaaacaatgg
     3001 aagaaagtca gaaaactgcc ttagcaactc aattacaaag acttgagaag caaaaagaag
     3061 gacttggaat tttgaaacaa agcttagaaa aagcgactga tcttttttct ggcgaggatg
     3121 aatttggcta ccataatacc tttatgaatt ttactaaaca atcccatgat attgaactgg
     3181 gtatcacaaa gactaacacc gaagtttcaa atcaagctaa tctttccaat agcagttcat
     3241 cagctattga acaagaaatt acaaaagttc aacaacaaat tggagaatat caagagttga
     3301 gagatgctat cataaataac agagcacgct taccaactgg caatccgcac cagtcaattt
     3361 tgaatcgtta tcttgtagcc tcacaaggac aaacacaagg aactgcagag gagccatttt
     3421 tatctcaaat taatcaaagt attgcaggtc ttgaatcatc tatcgcaagc ctcaaaattc
     3481 agcaagctgg tatcggaagt gtagcaactt atgataacag tttagcaacc aaaattgaag
     3541 tactccgcac tcagttttta cagacagcct cacagcaaca actaactgtg gagaatcaat
     3601 taacagaatt aaaagtacaa ctagatcaag ccacacagcg tttggaaaac aataccttaa
     3661 cctccccaag taaaggtatc gttcatctga acagcgaatt tgaaggtaaa aatagaattc
     3721 caactggtac agaaattgct caaatattcc ctgtcatcac agatacaaga gaagtactaa
     3781 tcacttacta cgtatcttct gactatctac ctctactaga taaaggacaa actgtaagat
     3841 taaaactgga gaagattgga aatcacggca ccaccatcat cggccaactt cagacaattg
     3901 atcaaactcc taccagaaca gagcaaggaa atctctttaa attaaccgct cttgcaaaac
     3961 tatctaacga ggatagtaaa ctcatccaat atggcttaca aggtcgcgtc actagtgtaa
     4021 ctacaaagaa aacatatttt gattatttca aagataaaat tttaacccat tctgattaat
     4081 tttcagataa cactctataa ctatttatta tcttatcaaa aaggagaatc ataacatgga
     4141 taagaaacaa aacctaactt catttcaaga actaacaact accgaactca accaaattac
     4201 aggtggagga ttgtgggaag atttattata taacattaat agatatgctc attacatcac
     4261 ataagaactt catcatccaa tacaactata aaaaaataag accgagaaac aagtactctc
     4321 ggtcttattt ttcatcattc tgtatgtatc acagtaagta cctgacgaaa gacttgattt
     4381 tgacaggtgg tatttagact ggtattagga tggctttcca caatcttcat gacggtatag
     4441 agaccaactc ctctctcctc ccctttagaa ctggctccaa aggagaagat ttcagaaata
     4501 tcgatgccct cttctttgat ggagttttcg atgataaagg tctcctgtgc tccatttttt
     4561 aaaaaggcga ttgaaacatg aggttgacta gcttccacac tggcttcaat agcattgtca
     4621 caaaggatag acacaatggt tagaaaatca agtagactca tcccctcgac ctgaatctcc
     4681 tcaggaactt cgacattaaa gacaatgttc ttatctctgg cttttaaaaa tttccctgct
     4741 agaagacttt tgagggcttt atcacgaata tttaccaatc tgcccaggtc atatttattg
     4801 ttctgcaatt tctgactgga atcctttaag acggagccat agacctcttt tatctgctcc
     4861 atatcctcct cttcaatgcc cagacgtaag ctagtcaaga ggttggtata atcatgacga
     4921 aagctccgta cttccttgta aagctcctct atatgccgac tatagcgttc catatctcta
     4981 tagcgcaggg cctgctcttg ttccaatctc tcatagagtt tttccttcaa ataggtatcc
     5041 aatttcttga taacccccat aaaaaagagt aggtaaaaga ctaggatgag atggcgaaca
     5101 gtctttgatt gaatactttg ttcatattca aaaaaagaca gactttccat gactagatag
     5161 tagccaccca ttatccagtt aatctgagtc agggactttt gaaaggcttt atcgagaatc
     5221 tcctttctca agctagtaaa atcgtagtcc aaccatttca aaaaagctag agaaatgaag
     5281 aaattgaaaa ttattataca taacccagta aatgagtagc catcatatac ttgcccttgt
     5341 cccaaaaatg gaagcacaaa ataggagact cctctataaa agagattcac caatatcatt
     5401 ggaaagagac cataaaagaa aaggagtttt ttaggaagcc ctctcaataa taagaaagat
     5461 aagcctatgc cgtacaaggg ttccataaaa taagataggt aaacatttcc tactatatag
     5521 ctaatcatca caaaaacaaa ggccaacagt atcttaaaaa gaaaggcctt aaaaatcctc
     5581 tcgaaagtaa gatcaattcc atccacctta aagaagatga caatttctag tccattagta
     5641 acaagtgtat acaacaatat ccaagcaatg ttcataaatt ctcctagctc agtgtaattt
     5701 attgatggcc tcagacactt ccctgacctt ataacgggcg attagacaac ttccaccatt
     5761 gggaaagaaa agcagttttt ctttcttatc caaatgcacc acatttgcag gattgatgag
     5821 aaaagagcgg tggcactgca agagacgggg ctcctgcttg aaaacctcct ctaaactcgc
     5881 tgtaaattcc agcctgtctg tcttggtata gagaataaca cgatgggctc tgggcgacgt
     5941 ttcgagatag taaacctctt taaaaggata ctgaaattgg gcaaattttg atttaaagta
     6001 aaagcaatct tccgccagac ttttactatc ttgactattg gcatagagga gggctgtctc
     6061 gatccgagat tcaaactcct ctgctgacaa ggccttatca atgtagtcca aagcagacac
     6121 ttggtagcga aaagacaggg gcataaactc cgagtgagtc gtcacaaaga caatcagggc
     6181 ataaggatcc cgatcccgaa tctttctagc cacttccagt cccttcatct cttcatttcg
     6241 aatctcaata tccaaaaaga atagctgatg ggcccccttc tcatgcactt cagccagcag
     6301 ttggtccggc ttgccaaata cctcaaaaga gctaggaatg atatgatgtg ctttcaaaag
     6361 tttctcaatc gtcgtttcaa ttctagtctg ttgggaaaaa tcatcctcta aaacaaatat
     6421 tctcatcttc ttactctccc tctttcaacc atttttgacg aatttctcta tagcgacgtc
     6481 tggcatactt gacagcgtat tgaccttctt ctccgagaaa aaggattctt tcttgaaaat
     6541 caatcgattt taatttatca agattaacca aacaatttcg atgacatctc atcaaggttt
     6601 ccccacattg gttctcaaga ttacttaaat tttgaagcat attaaaacta gcttcttctg
     6661 taacaatctg tacggtatgg gctttagttg gatgtgtttg gatatagtag atatcgtcga
     6721 tgtttacttt atagactgtt ttctgcgtct gaataatcat atatttcatt ggattctcct
     6781 cctaagaaaa gaataacaga aaaggtctag cccaagcaca cccaatccta aacagtcttt
     6841 gaaaactcct aaaaggatta ttttctacaa catcatgatg agcttacaac tattttatca
     6901 taaaattcac tcagcgcaat tcaagacatt tcaatgacaa ttaaagattt gaataaaaaa
     6961 aaagaattaa aagtgataaa ctaacagtat caaaacacag ttgccagaag taagactggc
     7021 acccagatta aaggaggcat tcccatgaca gatacagacc ccatcaaaag agctcatact
     7081 ttgattactg acttaaacaa agcctaccaa gcatgcaaac aggcaagcgc tgacgacgtc
     7141 cgctttcagg agcaattaaa ctctattctt ggttttctag ccaaggctga aacagtggat
     7201 aatcgattct tgattgaact agaaaaattt taccagactt ccagtcttct catgggactc
     7261 agcgctcttg atccagatgc tccaactcgc gccgcttggc gggcctatga ccgcttccac
     7321 tttgaccaag tcaagaccaa gttaatactc aatgaaaatc aaagagcaaa ctaggaagct
     7381 agccgcaagc tgtacttgag tacggtaaga cgacgctgac gtggtttgaa gagattttcg
     7441 aagagtataa gtctctacgg accaaccatt atcctgtagt aacaaaaaag aagttgagac
     7501 agactgaact caacttcttt tttagtgtcg tatcagcaat gttagtcctg catctgacgt
     7561 tttacctgat aaggcaaata caagactagt aaaaccaaac cagctcctag taaggctaga
     7621 agggctagtt tttcttggaa ggtaatcaac ccaacaacta attccacaag taaaacaaaa
     7681 ctggtcaatc ctcgaactac ctcctgtaag cctttttcct tttgcccctt gtccagcgga
     7741 aagagttggg tcaaatactg gtagtcaaag gcatgataga gggccagcaa ctggaagagc
     7801 aagaggtagt taaagagaac taccactgct gtcgcaatcc aagcttgctc gataaaaacc
     7861 tgcgccagta cggaaagtaa gagaagacga agactaagag caaagaggtc gccatttcgc
     7921 agataagaac gcagatagag attttgccaa atcttcccag gcaccttctg aacagccttt
     7981 aagataaagt ccagataggc acgacgctta acgctgtttg aaattccctt gacctgcgta
     8041 aagagggcaa agaaacgaag caagacttgc ttacgcttgc tttcttggga aatgacatag
     8101 tcccaatcca gtccagtttc agtgaaaaat ttgctggcct tttgacgaaa gtggaaatat
     8161 tttcctaccc ccaataaaag cacatagagc agaaaaactg gcaagccata acccattgct
     8221 aaaaataaag gcgcaaataa cagcaagaaa agggtctgta caaagagcca aaagactagg
     8281 gaaatgccag tttgacgctt gagatggagc ttgatttcct cttctccaac taagagaaag
     8341 agcttgtctg gagcctccat ataggtggca attcctcccc aaagtaaaag taaaacagac
     8401 gtaattccta caaacaaaag gataggccaa tgattttcag gaaaatgttg taagagttga
     8461 ctgtactggt aggctagaaa gcccaacagg acaagcagga acaagacaaa gtggtcattg
     8521 agcacatagc gcagataacc aagacactcc ttacgaaagg cctgctttct ctttaaaaac
     8581 aagtctttca taggtcctcc tcttttgtca gagccaagta aatatcattc aaactagcct
     8641 caggcatatc aaaggcttcg cgtagttgca ggagattgcc tttggcacgc acctctccct
     8701 tgtgaagaat gacaaaggca tcacacatct tctccgccga atccagcacg tgggtactca
     8761 tgagaataga cttgcccttt tgcttctcca cttccaaaag ctgaatcaaa tcagaaatag
     8821 ccagcggatc aagaccaagg aaaggctcat ccacgatgaa aagacttgga tccgccacaa
     8881 aagcacagat aatcatgacc ttctgcttca tcccttttga aaaatgaaca gggaaccagt
     8941 ctaatttctg ttccaaacgg aacattttta acaagggctc tactcgttcg aaagccactt
     9001 tttgctcaat accgtaagcc atagcaaccg tttcgatatg ctctctgagg gtcaattcct
     9061 catacagact aggcgtctca ggaatgtagc caatctgctt gcggtagcta gtcgcatctc
     9121 cttgcagagt caggccattg atattgatgg agccactata aggtgccaac agaccgataa
     9181 tctcattgat cgtcgttgat ttcccagcac cattgagacc aatcaaaccg accaactgcc
     9241 cactttcaac agtaaaggac acatctttca aaacaggaac atgaacatag ccacctgtca
     9301 ggtttttaat ttctaacata ttttctccaa atctggtata atgtagctat attatatcaa
     9361 aattcagtac agtagaggta gattttatgt cagattgcat tttttgtaaa atcatcgcag
     9421 gggaaattcc tgcttcaaaa gtatatgaag atgagcaggt ccttgccttt cttgatatct
     9481 ctcaagtaac actaggacac accttggtcg tgccaaaaga acactatcgc aatcttttgg
     9541 agatggatgc tacgagcgcc agccaactct ttgcccaagt accaaaagta gctcaaaaag
     9601 tcatgaaagt cactaaggct gctggtatga atatcatttc caactgtgaa gaagtcgctg
     9661 gtcaaacagt ttttcatact cacgttcacc ttgtacctcg ctacagtgct gacgatgacc
     9721 tcaagattga ttttatcgcc cacgaaccag actttgacaa acttgctcaa gtcgctgaaa
     9781 ccatcaaaaa cgcttaagga gttgctatga aattatccaa cctactgcta tttgcaggag
     9841 ctgcagccgg aagttatctg gtcacaaaaa atcgccaaac catcacagat gaagtcttga
     9901 ataccactga ccgcgttcaa gctatcaagg acgatgtgga tattatccaa aacagcctgc
     9961 aaatcattga ccagcaaaaa gaacttatca aggaatacca agaagacttg acttacaagt
    10021 ttaaggtctt ggaaaaggat atccagacta gactagctgt gataaaagaa atgcagggaa
    10081 ctgaagataa gtaaaaagag cccgacggct cttttttaaa acgtaaacaa gttatagtga
    10141 attgaattta gaatagcaca tcatagtttc taaagcattg ttagagatta ctttaaaatc
    10201 ctcttatcaa tttgttcata ttctatttca atctactata tatatcccat caactatgca
    10261 tcataattta ggtaactcat actcaataaa aatcaaaaag caaactagaa agctaggcac
    10321 agactgctca aaacaccgtt ttaaggttgt ggatagaact gacgaagtca gtaaccatac
    10381 ccatgacaag ccgacgctga cgtggtttga agaaattttt gaagagtatc attcaccatt
    10441 tcacaactaa atcgaagact ttctgccgta atgaaaacac ctgaaacagc ttggtttcgg
    10501 ctatccggaa actttaagac ttaggttcaa agtttaggta tggaacttcg aaggaggtcg
    10561 ctaccgtccg tcattactta ggaaagtctt aaaaaatcta taaagtaaaa agagcccgac
    10621 agctcttttt gtttatactc aatgaaaatc aaaaagcaaa ctaggaaact agccacgggc
    10681 tgctcaaaac actgttttga ggttgtagat acgactgacg aagtcagtca catatatacg
    10741 gcaaggcaac gctgacgtgg tttgaagaga ttttcgaaga gtattattct ccatcaaagg
    10801 catctttaat atggtcaaag aagccttttt tctttggatt tactttcaag tcaccagcag
    10861 ccgcgaattc tttcaaggct actttttggc ggtcgttcaa gcctgtcggt gttacgacat
    10921 taacagtaac gtattggtca ccaactgcac cgccacgaag gctcggtgcc cccttactac
    10981 gtaggcggaa cttcttacca gtctgagttc cctctggaat aaccaattca acatcaccgt
    11041 gaacagttgg aatatctact gtatcaccaa gagccgcttg gacaaagttg aggttgagat
    11101 tgtagaagat agtcgttcct tcacgttcaa acttgtcgct agcttccaca gaaactacta
    11161 catacaagtc accataaggt ccaccgttaa agcctgcttc accttgacca gcgaggcgaa
    11221 tttgttgacc tgtttccaca ccagcaggga ttttcacatg tacgctatga gcttgtttct
    11281 catgacctgt tccatgacag gttgtacatg gatatttgat ttcttttcct cgaccgtgac
    11341 agacatcaca ggttacttgg cgacgcatca taccaagagg agtctgcgta tcgacgttaa
    11401 tgacaccagc gccatgacag cgtccacaag tgactggact tgtccctggc ttagcaccag
    11461 atccattaca tgtacgacag ccagcttcac gatgatactt aacttccttc tcagttccga
    11521 agatagcttc ttcaaaggtc aaattgacac gatactggag atcatctcct tggcgaggag
    11581 cgtttggatt gcgcgaagaa ccgcctccgc cgaagaaact tgagaaaata tcctcaaaac
    11641 caccgaagcc acctgcccca ttgaaaccgc cgaaaccacc agctccacca aaaccaccat
    11701 tggcgcctgc agcaccatac tggtcatagg cagcacgttt ttggtcgtca ctcaaagtct
    11761 cataggcttc ttgaacttcc ttgtacttgt cctcagcacc aggctccttg ttgatatctg
    11821 ggtgatattt tttggaaagc ttacgataag cctttttgat ttcgtctgcc gaagcgtttt
    11881 tggatacccc cagacgatca taaaattcag tattgttcat aattcaagat acaaagggcg
    11941 tcaaacggac acagccatct aggagttttg acgacggacg cttacgtcct agaaaaaact
    12001 atctttttgg cacagtccat agcccgtgtt cagttacgaa cacccttatt cctttctatt
    12061 gatgttctga atcgaaccac catttaggtc gggttcaatt cctttctttt atatttagta
    12121 agaaattctg aaacccgtgt tcagttacga acactctttg ttcctttcta ttgatgagac
    12181 aaaacccaaa caaatgagat tccgtccctt tttatcgaaa aacagaggct gggttgcaac
    12241 ctctgcaaat aagtaaatta actttatttt gattttaaat atcctataag tacagaaata
    12301 tttatatatt tttgtctctt cttagtattt gcctatataa ttccaattga tcataagaaa
    12361 tacaactact gtccaaatca taccaattat gaacggtatt acaaatttat atgtttgtat
    12421 ttctaatgat atcatagctg ataccatcgc tgatacaaat gcagacaaca atactgaagt
    12481 aacaaaattc cttaccgctt catcattttt gacacaacta tcatgctcag ctaatataat
    12541 ttctctatat atagtcaaac attctactgg aatattatct ataaaacctt ttgctgcttc
    12601 atagttgtca gcttgattac aaaaatcatt ataaactttt cgtgtatttt ttagatactc
    12661 atcatccaat acactcatct tatttttccg taaactctcc gtctacgacg tcatcgcctg
    12721 cgtttcctgt tgcttgtgcg ccttctgctc cttcttgagc ttgttgcgct gctgcggctt
    12781 gttcgtagag tttcacagca aggccttgag ctttttcgtt caacgcttca agtttcgctt
    12841 tcatgtcgtc caagttgttg tcttcttgag ctttcttaag gtcatcaagg gcagcttggg
    12901 cagcgtcacg ttctgcgtcg aagcctttac cttcagtttc cttgattgtc ttttcagtcg
    12961 caaagattgc ttggtctact tcattacgaa ggtctacttc ttctttacgt ttcttatcgg
    13021 cttcagcgtt tgcttctgca tctttcatca tgcggtcgat ttcttcgtca gtcaaacctg
    13081 agttcgattg gatgacaata gtttgttctt tttgagttcc aaggtctttg gccttaacag
    13141 acacgatacc gttcttgtcg atgtcaaatg ttacttcgat ttgaggaatt ccacgaggtg
    13201 cagctgggat atcagtcaat tggaagcgtc caagagtctt gttatctgct gccattgggc
    13261 gttcaccttg aagaacgtgg atatcaacgg ctggttggtt gtctgctgct gttgagaaga
    13321 cttgtgattt agatgttggg attgtagtgt tgcgatcgat aagttttgta aatactccac
    13381 ccattgtttc gataccaagt gacaatggcg ttacatcaag aaggacaacg tccttgacat
    13441 caccagtaat cacaccacct tggatagccg cacccatagc aactacttca tcagggttta
    13501 ctgatttgtt tggttcttta ccagtttcag ctttaacagc ttcaacaacg gcagggatac
    13561 gagttgaacc accaacaagg ataacttcgt cgatttctga caagctcaaa cctgcatctg
    13621 aaagggcttg acgaactgga acttttgtac gttcaacaag gtcacgagtc aaatcgtcaa
    13681 atttcgcacg agtcaaagtc atttccaagt gaagaggtcc agcctcacct gcagtgataa
    13741 atggcaagct gatttgtgtt gaagttacac cagaaaggtc tttcttcgct ttttcagccg
    13801 catctttcaa acgttgcatt gccatcttgt cagtagacaa gtcgataccg ttttctttct
    13861 tgaattctgc taccaagtgg tcaatgattt tttggtcaaa gtcgtcacca ccaagtttgt
    13921 tgtcccctgc agttgacaat acgtcgaaga caccgtcacc caattcaagg atagagacgt
    13981 cgaatgtacc accaccaagg tcaaatacca agattttttc ttctttatca gtcttgtcca
    14041 aaccataagc aagagctgct gcagttggtt cgttaacaat acgttctact tcaagaccag
    14101 caattttacc agcgtctttt gttgcttgac gttgagcgtc gttgaagtaa gccggaactg
    14161 tgataacagc tttggttact ttctcaccaa ggtagtcttc agcgtagcct ttcaagtatt
    14221 gaaggatcat agctgagatt tcttgtggag tgtattcttt tccatttgca gaaacttttt
    14281 cagaagttcc catcttagat ttgatagaga taactgtatc tgggtttgtg actgcttgac
    14341 gttttgcagc atcaccaacg atgatttctc cgtttttgaa tgagactaca gatggagttg
    14401 tgcggtttcc ttctgggttt gcgatgattt tgctttcagt tccttcaaga actgcaactg
    14461 ctgagtttgt tgtacctaag tcaataccga taattttaga catgtgtttt tctccttgtt
    14521 agttaatttt ctatttttct ttttgatact cttcttaagt ggcgacgccg tcagagcttg
    14581 acttcgtcaa tctctttgac taaactttga gcctaaggtc tcaaaatttg cgcaaatagc
    14641 gccacggcga agagtatcgt gtttggttcg cttcgctcac tattacaaag ccaaacatat
    14701 tttatctttc ttcatagttt attgtcgttt cggacaagtt ttctattatg taaattgcga
    14761 cacgaagagt cgaaatcgat tttatttcga cgacgagtta gtaaggaagc taggcaaacg
    14821 ccatagcgat tgccgttttc tgacgagttg ctttagctac cgtcagaatt gccgaatcga
    14881 acacgcccta agctctgagt aaaaaagata gattttcttg tgttcatcga acacttcgtt
    14941 aatctcctat ttttactgcg agctttttac gggctttgta tcttagttat acaccactac
    15001 cattgctggg cgtaggatgc ggtcatggag tttgtagcct ttttgaaaga cttgagcgat
    15061 ggtatctact gggtgttcat cgtctgctgg gagagtttgg atggccatat ggtagttatg
    15121 gtcaaattcg ccatctgctg cgatttcttc aattccttct tctttcaaag cgtgaatcaa
    15181 gctttcttgc accatcccca agcccttctt cacatcatct gtcaaacctt caactgcaag
    15241 tgcacgctca aggttgtcaa gagatggtaa gattgctttt gccaagtcct ggctacgata
    15301 acgttgcaag ttttgacgtt cttcattggc acggcgttgg atattttgca tttctgcatg
    15361 agcgcgaaga tatttgtttt cgaactcatc tgcacgttca tttgccaagt ccaactcaga
    15421 cttttcagga gttgtttctt cagctgtttc cacaacttcc tcttcttgaa cttcttctac
    15481 ttcttcattt tttatatctt gggccatgtt cgcctccttt aatgatttca atcttaatgt
    15541 acttcgtaat gattactgct gaggtagcgg taaaaatctg tcaacttcat ggtcaaaaca
    15601 cggttgacca cattgatttg attgattagc tgttggtaat ccagattaac tggaccgata
    15661 atggctagaa ttccaactcc ccgataagga atgaggaact tactactaat caccgctagg
    15721 tcagctaaac aggactcttg accgtctgca atacgaacat tttgcatctg atcctcacgc
    15781 aacccctcac gaatctccaa ggccaccttt tgcggttggt caaagaactg ataggctgct
    15841 agattggcaa aattcaagag attgaccttg cccgccatca caatgttttc gttgaacatt
    15901 tccttaaaga tgtgttcaaa gagatcgatg acattatccg ttgttgtaaa gtaacgctgg
    15961 ataatctgcg gaatctccgt ccgaatcttg tagtgaatat ctaaaacggt gtgaccgagg
    16021 aaacgttcct gaatgatgct cttcagtttc agcaaatcct cctgcaagaa gttccttgga
    16081 atcagaaact gactagtaac cgttcgcgac tcgtctaggg taaataccgc caaggctgta
    16141 tgttgcccca aaacaacgat atcaaaggct gtcaaacgtt gcctgctcgg ctcaacatcc
    16201 agtgccacta ccgtacagcc actcaggtct gttagcaagt tagcagcctc ttgcagaata
    16261 tcctccaatt tgaagaattc ctgatcaaag gctttgacaa tctcatatac ctcattttca
    16321 gccagccggt gaaaatccag tgagtgtttc acatagtact gaaaaccagc aacacttggc
    16381 atccgaccgc ttgaagtatg agccttctca agcaaccctt gtttttctag ttccgccatg
    16441 tcattacgaa tggttgcact gctagagtta atagactctt gcaaggcttt tgatccgaca
    16501 ggttcgtgcg ttttggtaaa gatgtcaata atcagattta aaatatcctg ctgacgctct
    16561 gtaaccatct catcactcct ctctgtctga tcaattagca ctcaatgcat ccaagtgcta
    16621 acttatgatt ctattataca cccttagaag agaatgtcaa gacaaaaact caaaaaatta
    16681 gcactctttt atcaagagtg ctaaaaatca atctgaagac ctagaacctt gctattcatc
    16741 tactcggtat ttccttttta gtctgaaata accatgaatt agataagaaa tcagaaaagc
    16801 atataaaaca aaaataagaa agaaagacat agaaagatcc tctctaaaca aactcgtaca
    16861 aacaacaaga ccacctaaga aagtagttgt acaggaacga agtctagatc gcataacttc
    16921 ccatttgagc cccttactca tatagacttg atcctctgga agtaaatcag aagacgattt
    16981 acgaagaatc gaacaagaac ctgctcctat caattcccaa cctctatctc taaaatcttg
    17041 cagttcatgc ttatattttt taagaaatct agaatcatag atacggtaga tgacatcgtc
    17101 tggttgacat tggtcaaaat agaacaaacc aaaacgactc gttctatacc tccaaccttt
    17161 caaatgcatc tcatgtaaat attcttcttc cttgtccaaa tcaacaatgg tgaaaatccg
    17221 aaattctact ctgctattca ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt
    17281 tattagataa ttctttccac ttttgactca atctccaaaa aatataagaa atctgaatcg
    17341 caaaaactat caataaaacc caatctatta tgaaaatcaa aaacactttc caactgaaag
    17401 aactacctcc agtgacaaac tttgagcaaa acggtagtag agctaaaaag agaagtaaaa
    17461 taggaagcat ccgcattgtt aaaatccgtt tggcataaaa aaatctttat ttaaactaaa
    17521 atattatagc aaaattta
//
LOCUS       DAYYQN010000041        17003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYYQN010000041 DAYYQN010000000
VERSION     DAYYQN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.041"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..653
                     /gene="erm"
                     /locus_tag="ACHBRQ_001421"
     CDS             <1..653
                     /gene="erm"
                     /locus_tag="ACHBRQ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFM9593072.1"
                     /translation="TDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKL
                     NTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIYLIVEEGFY
                     KRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKL
                     YTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(910..2157)
                     /locus_tag="ACHBRQ_001422"
     CDS             complement(910..2157)
                     /locus_tag="ACHBRQ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9593073.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            2337..2558
                     /locus_tag="ACHBRQ_001423"
     CDS             2337..2558
                     /locus_tag="ACHBRQ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593074.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            2675..3172
                     /locus_tag="ACHBRQ_001424"
     CDS             2675..3172
                     /locus_tag="ACHBRQ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9593075.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3261..3653
                     /locus_tag="ACHBRQ_001425"
     CDS             3261..3653
                     /locus_tag="ACHBRQ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9593076.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            3637..6084
                     /locus_tag="ACHBRQ_001426"
     CDS             3637..6084
                     /locus_tag="ACHBRQ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9593077.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6087..8264
                     /locus_tag="ACHBRQ_001427"
     CDS             6087..8264
                     /locus_tag="ACHBRQ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9593078.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8261..9262
                     /locus_tag="ACHBRQ_001428"
     CDS             8261..9262
                     /locus_tag="ACHBRQ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9593079.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9259..10194
                     /locus_tag="ACHBRQ_001429"
     CDS             9259..10194
                     /locus_tag="ACHBRQ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9593080.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10508..10555
                     /locus_tag="ACHBRQ_001430"
                     /pseudo
     CDS             10508..10555
                     /locus_tag="ACHBRQ_001430"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10571..12490
                     /gene="tet(M)"
                     /locus_tag="ACHBRQ_001431"
     CDS             10571..12490
                     /gene="tet(M)"
                     /locus_tag="ACHBRQ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM9593081.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            12609..12776
                     /locus_tag="ACHBRQ_001432"
     CDS             12609..12776
                     /locus_tag="ACHBRQ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9593082.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(12836..13189)
                     /locus_tag="ACHBRQ_001433"
     CDS             complement(12836..13189)
                     /locus_tag="ACHBRQ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9593083.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            13694..14116
                     /locus_tag="ACHBRQ_001434"
     CDS             13694..14116
                     /locus_tag="ACHBRQ_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9593084.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            14113..14343
                     /locus_tag="ACHBRQ_001435"
     CDS             14113..14343
                     /locus_tag="ACHBRQ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9593085.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(14569..14820)
                     /locus_tag="ACHBRQ_001436"
     CDS             complement(14569..14820)
                     /locus_tag="ACHBRQ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593086.1"
                     /translation="MSVCFIKSSFPNHLFGFHKSCFTSNRTALAKLTGVPPLHGSHVA
                     ILIACDGFITLGLLTVWEYHYHDKNVVAGNPAKDCFSDH"
     gene            14804..15007
                     /locus_tag="ACHBRQ_001437"
     CDS             14804..15007
                     /locus_tag="ACHBRQ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9593087.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            15089..16306
                     /locus_tag="ACHBRQ_001438"
     CDS             15089..16306
                     /locus_tag="ACHBRQ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9593088.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(16468..>17003)
                     /locus_tag="ACHBRQ_001439"
     CDS             complement(16468..>17003)
                     /locus_tag="ACHBRQ_001439"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFM9593089.1"
                     /translation="KFLCIGELTILQIIYGASLISFLWMYGQRKQVAKVNMETRIKWI
                     GIGFVSLLIISLYFSLIHAQESTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYREI
                     LWNLVRKLDIRVALTSVLFALAHHPGTILAWCLYVSLGMFLGMVRYKSDLWGSMGLHL
                     VWNLLVYSLLLFNKNKV"
BASE COUNT         5574 a         3035 c         3436 g         4958 t
ORIGIN      
        1 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
       61 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      121 aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat
      181 tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta agcacacaaa
      241 ttattaaaaa agtggttttt gaaagccgtg cgtctgacat ctatctgatt gttgaagaag
      301 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      361 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      421 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      481 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      541 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc attacttatg
      601 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
      661 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
      721 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
      781 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
      841 aagtgggata tctgtcaact ggtatggttg actaaaaata cttcctacga aaatgtaggg
      901 ggtatttttt tacgaaaaaa tacaatcgat tcttaaaaag aaaaattttt gattggcaaa
      961 accataacaa gttcgtttta gggttttgat tttgcgattg atgccttcta aaggaccatt
     1021 agagtattca aatttagcgc tatttaagac atattttctg ttttgacgaa gggtttgaat
     1081 agcagtatcc atttctgtat tggttttttg gtagtctaag atggttgact ctagtaattc
     1141 actattgcgc tcgtttaggg ctttcgtgat atcttggtaa gtttggtata cttcagcgaa
     1201 cttggaaaat ttactagtaa tgagatcaac agcattttgg cgagtcatat attgtttaac
     1261 gccgcgaaga aaaactactt cttcagggtg aagatcttca gcttttttat ggaatagctt
     1321 ccaatgtgac ttcataattt tatattcttg gctctgttta tcaagttgct ttaggataga
     1381 gatacgacaa ttgtccaaag cgcgaccagc taattgtaca aggtggaagc gatcaataat
     1441 gatattggca ttagggaaaa ggcgatagat aaaactttga tattgagcat ttaaatcaat
     1501 tacaactgat tgaacgcatt cgcgttcggc ttttgaataa cgactttcaa aataatcaac
     1561 aatggtaggt gatagacgat cctgtaactt tgtgacaatt tggtgggttt cagcgtcaca
     1621 acagataaag gacatcacag acttaattga acgaaactcg tcaaaacata gatgcttagg
     1681 caacttagcc acacgatagt gtggttccat gcgctctaag attgttcgac gaacactgct
     1741 aggagagcag tgacacattt cagcaataag ctgaccagat aagcctttac gagctaaaag
     1801 catgatttga tttttgagat cactggataa ggtttgattt tctttggtta aattagtaat
     1861 agcaccaaaa gtagtatggc atgatttaca tttatagcgt tgtttacgaa gctctagttc
     1921 atatcttctc ccatttaaac ttgccagtcg tacatgagtt ttgcgaaagc catccttatt
     1981 aactgtggga aagccacagt tacgacaacg attaatcgga taagaaagag tagctgttat
     2041 tagcgttata tactctttaa cagaatcgtt gttgtgttca gcttcttcaa cagaaataat
     2101 tttaatattt ttatctttaa ttccaagaat atttaggata gaatcattat gggacatttg
     2161 tttaaccttc tttcatgatt tttgtggtga attgattgta taacgagggg acagcaaatg
     2221 tcctcttttt tgtataaaaa aatctggcat ggaatctcta tccataccag aaagtgtata
     2281 ccccaaaaaa ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga
     2341 attttggaca aaacctttat aactggtttc tatcaaacgc tcaatcactg gtgcttttag
     2401 caatcgttgt gattggcttg tatcttggct tcaagcgtga gtttagcaaa ctgattggct
     2461 ttttaattat tgcgattatt gcggttggct tagtcttcaa cgctgctgga gtaaaagaca
     2521 ttttactaga gctattcaat cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa
     2581 tataagtggt tttcttatgt tccgcaaagg aatggtacac caaacgaagt gcggtaggga
     2641 tttttgaatc tctacaaaga aaggacgtga atatatggac gatatgcaag tctatattgc
     2701 gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg tggtttacct ttcccattga
     2761 ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa tatgaggaat acgccattca
     2821 tgactacgag ttacccttta cggttgacga atacacttcc attggcgaac tcaatcgact
     2881 atgggaaatg gtatcggaat tacccgaaga attacaatcg gagctatctg ctctgctcac
     2941 tcatttttca agcattgaag aactaagcga acatcaagag gatattatca ttcattccga
     3001 ttgtgatgat atgtatgacg tggcacgcta ctacattgaa gaaacgggtg ctttaggcga
     3061 agtaccagct agtcttcaaa actatattga ttatcaagcc tatggtcggg atttagacct
     3121 ttcaggaacg tttatctcaa ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg
     3181 gtacattact actggcagat tttctatttt acggggtggc tcaatcagct acccctattt
     3241 tttatgaaag gattgattac atgaagaaaa tacgaagcta taccagtatc tggtctgtgg
     3301 aaaaggtact gtattctatc aatgatttta gacttccgtt tcccataacc tttacgcaaa
     3361 tgacatggtt tgtcgtgtca ctctttgcag tgatgatact tggcaacttg ccccctcttt
     3421 ccatgataga gggagcattt ctcaaatact ttgggattcc tgtggctttc acatggttta
     3481 tgtctacaaa aacttttgat ggtaaaaagc cttatggatt tttgaagtct gtcattgctt
     3541 atgcactgcg accaaagctg acctatgcag gaaaaaaagt aacgcttggc agaaaccagc
     3601 cacaagaagc cattacagca gttaggagtg aattttatgg catatccaat taaatacatt
     3661 gaaaacaatc tcgtctggaa taaagacggg gaatgttatg cttactatga gcttgttcct
     3721 tacaattact catttctaag tccagaacag aaaatacaag tgcatgattc tttcagacag
     3781 cttatcgcac aaaatcgtga tggcaaaatt catgctttac aaatcagtac agaatccagc
     3841 atacgttctg cacaagagcg ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc
     3901 tatgacaaaa tcgaccaaca gacagacgct ttaatatcca tgattggcga aaatcaagtg
     3961 aactaccgtt tctttatcgg ctttaagttg cttctcaacg atcaggagtt ttctatgaaa
     4021 agtcttaccg ttgaagcaaa aaatgctttg tctgattttg tctatgatgt gaaccataag
     4081 ctgatgggcg attttgttag tatgagtaat gatgaaatcc tgcgttttca gaagatggaa
     4141 aagctcttag aaaataaaat ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac
     4201 ttcggctatc tgattgaaca cctttacgga cagacaggca ctgcctatga agagtatgag
     4261 taccatctat caaagaaaaa gctggataat gaaacgctga ttaaatacta tgacttgatt
     4321 aagcctactc gctgtttggt ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat
     4381 gaaaccgtct atgtagctta ctttaccatt aacagcattg tcggagaact ggacttcccg
     4441 tcctctgaaa tcttctacta ccagcaacag caatttacat tcccgattga tacgtcaatg
     4501 aatgtggaaa ttgtagcgaa tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa
     4561 ctgaaagact tggataacca cgcttggcaa agtgataatg aaaccagctc caatgtggcg
     4621 gaagctctgg aaagtgtgaa tgagctggaa accaatttag accaaagcaa ggaatctatg
     4681 tacaagctgt cttatgtggt aagggtatca gcaaatgatc ttgacgaact caaacgtcgt
     4741 tgtaatgaag tgaaagattt ttatgacgat ttaagcgtaa aactggtacg accatttggg
     4801 gatatgctcg gcttacatga agaattttta cctgccagca agcgttatat gaatgattat
     4861 attcaatacg tgacctctga tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg
     4921 ggggaaaatg aggggattta tgttggctac agcttagata ctggacgcaa tgtctatctg
     4981 aaacctgctc ttgccagtca aggggttaag ggttcagtaa ccaatgcgtt agcgtcggct
     5041 tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgcg
     5101 gtgctttatg gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa
     5161 gaaaccttgc cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa
     5221 aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg
     5281 gctattgata ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca
     5341 atccttagaa aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg
     5401 attgaggaat tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa
     5461 agttttacag actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct
     5521 atcagcttag aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag
     5581 gaaacttcct ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc
     5641 attagtacct ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat
     5701 ttagacgaag catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg
     5761 gttcgggctg gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac
     5821 ctcttagatg aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac
     5881 cttaacgaga ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat
     5941 cagaagcgat tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt
     6001 gtcggtgtga tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga
     6061 ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa
     6121 actggacgct gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg
     6181 ccatttttct tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata
     6241 cggtcaatgt ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt
     6301 atgtggataa tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg
     6361 tcatgtatgg actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca
     6421 atgcgacagg ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag
     6481 attccattgg taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag
     6541 agggtttcta tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct
     6601 atacgggact gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc
     6661 tggtgtttat cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca
     6721 atgacttttc atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc
     6781 cccattccga tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca
     6841 tacaggttca gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg
     6901 gtattgaccg tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca
     6961 gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa
     7021 agaccattaa ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca
     7081 tatttgtatt cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg
     7141 ctatgtttct gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa
     7201 aacgagccat aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta
     7261 ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct
     7321 ttttgattgc ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt
     7381 taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga
     7441 gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt
     7501 ccatgactac tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt
     7561 cggggagttc tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa
     7621 cacttggaaa acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg
     7681 tagacactgc tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg
     7741 cagtatatca aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt
     7801 ctcaaaccaa agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa
     7861 ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt
     7921 ctgttcatga aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac
     7981 agtctaatat tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag
     8041 ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa
     8101 ccgttcaaaa gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca
     8161 caaaagaacg tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac
     8221 caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt
     8281 gattggtggt tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt
     8341 attttcagat gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc
     8401 cgcagaagtg ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga
     8461 agaatatgtc aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga
     8521 tgttatgcag tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc
     8581 cattaagcaa ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt
     8641 agatttagaa tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc
     8701 taatcgtgga aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg
     8761 tggcgaaaaa gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata
     8821 caactatggc aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt
     8881 tgatgatgat acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata
     8941 cgtttacggt ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac
     9001 gtatggaaaa gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca
     9061 gcatatccca ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa
     9121 cgctggctct tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc
     9181 aggcgaccca atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg
     9241 agcaggacga atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag
     9301 aataaagaaa aacagatacc aaaggaaaag aaacctcgtg tctactataa ggtcaatcct
     9361 cataaaaagg ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata
     9421 ttcaagcact ttacagctat agatactcat actattcacg aaacaactat catagaaaag
     9481 gaatacgttg atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat
     9541 tcatgggagc aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg
     9601 acagatgaac ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct
     9661 tctgtaagag gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta
     9721 acctacagtg tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct
     9781 tatatagtga gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc
     9841 attaccaaca tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg
     9901 gttgattcca ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat
     9961 cctacagcga cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc
    10021 ggaaaagagt acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa
    10081 gtcacggtat cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct
    10141 caatttgatt tggtacttga aaagaacggg agtaattgga agattataga ataacaaata
    10201 ttggtacatt attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat
    10261 tcctttacaa atatgctctt atgtgctatt atttaagtat ctatttaaaa ggagttaata
    10321 aatatgcggc aaggtattct taaataaact gtcaatttga tagtggaaac aaataattgg
    10381 atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt
    10441 gattctaaag tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa
    10501 tcccagtgat aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga
    10561 ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg cgggaaaaac
    10621 taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga
    10681 cagaggtaca acgaaaacgg ataatacgct tttagaacgt cagagaggaa ttacaattca
    10741 gacggcgata acctcttttc agtggaaaaa tactaagata aacatcatag acacgccagg
    10801 acatatggat tttttagcag aagtatatcg ttcattatca gtattagatg gggcaattct
    10861 actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattgtttc atgcacttag
    10921 gaaaataggt attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt
    10981 atcaacggtt tatcaggata ttaaagagaa actttctgcg gaaattgtaa tcaaacagaa
    11041 ggtagaactg catcctaata tgcgtgtaat gaactttacc gaatctgaac aatgggatat
    11101 ggtaatagaa ggaaatgatt accttttgga gaaatatacg tctgggaaat tattggaagc
    11161 attagaactc gaacaagagg aaagcataag atttcataat tgttccctgt tccctgttta
    11221 tcacggaagt gcaaaaaaca atatagggat tgataacctt atagaagtga ttacgaataa
    11281 attttattca tcaacacatc gaggtcagtc tgaactttgc ggaaaagttt tcaaaattga
    11341 gtattcggaa aaaagacagc gtcttgcata tatacgtctt tatagtggcg tactgcattt
    11401 gcgagattcg gttagaatat cggaaaagga aaaaataaaa attacagaaa tgtatacttc
    11461 aataaatggt gaattatgta aaatcgataa ggcttattcc ggggaaattg ttattttgca
    11521 gaatgagttt ttgaagttaa atagtgttct tggagataca aagctattgc cacagagaga
    11581 gagaattgaa aatcccctcc ctctgctgca aacgactgtt gaaccgagca aacctcaaca
    11641 aagggaaatg ttacttgatg cacttttaga aatctccgac agtgacccgc ttctgcgata
    11701 ttatgtggat tctgcgacac atgaaatcat actttctttc ttagggaaag tacaaatgga
    11761 agtgacttgt gctctgctgc aagaaaagta tcatgtggag atagaaataa aagagcctac
    11821 agtcatttat atggaaagac cgttaaaaaa agcagagtat accattcaca tcgaagttcc
    11881 accgaatcct ttctgggctt ccattggtct atctgtagca ccgcttccat tagggagcgg
    11941 agtacagtat gagagctcgg tttctcttgg atacttaaat caatcgtttc aaaatgcagt
    12001 tatggagggg atacgctatg gctgtgaaca aggattgtat ggttggaatg tgacggactg
    12061 taaaatctgt tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg
    12121 gatgcttgct cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga
    12181 gccatatctt agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga
    12241 tgctcctaaa tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct
    12301 tagtggagaa atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac
    12361 aaatggacgt agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc
    12421 tgtttgccag ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa
    12481 aataacttag tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa
    12541 tattttttaa taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca
    12601 agtggatatt gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg
    12661 aattaaaaaa attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa
    12721 agcagttcaa agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg
    12781 agattaatac aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt
    12841 cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga ttccattaag gatagttctt
    12901 tgtctgtaaa gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt
    12961 tattagcagg taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa
    13021 gaacttgtat gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa
    13081 tttcaatcaa tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa
    13141 tggctaaacc aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc
    13201 tctatacatt ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg
    13261 tttataggtt tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat
    13321 ggtagtgcta tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga
    13381 ggtcaacgtc gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc
    13441 aaaaaaagtc gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat
    13501 atagatagaa taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg
    13561 ctattcagaa cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca
    13621 ttgggactaa gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa
    13681 gggggtgaga aacatgaaac catcttcttt tcagaccaca atagaaaatc agtttgacta
    13741 tatctgtaaa cgtgctatgg aagacgagcg aaagaattat atgctttatc tttcaaggat
    13801 tgcaaagcgt gaggtgtcct tttcggatgt tggcgattat cttgttagcc agtttgcgac
    13861 aacagataac tattcaactg actttcagat ttttacactc aatgggttat cagtaggcgt
    13921 tgaaaatgat ttgttgagtg aagcattacg tgagttgcca gacaagaaac gtgaaattct
    13981 actgctgttt tactttatgg acatgagcga ttcagaaatt gcagacctgt tgaaattgaa
    14041 ccgttctact gtctatcggc atagaaccag tggactagcc ttaattaaaa agtttatgga
    14101 ggaatttgaa gaatgaaaac acaatatcct atgattccct ttcctctcat tgtaaaggca
    14161 acagatggcg ataccgaagc gattaaccag attctacatc attacagagg gtacataacg
    14221 aagcgttccc tacgacttat gaaagatgaa tatggcaatc aaagtatggt cgttgatgaa
    14281 gtcttacgtg gaagaatgga aaccagactg attacaaaga ttttgtcatt tgaaattaag
    14341 taatatcctc tctcctttcg tggaagcgtg ctaaaccatt ccacgcttcc cgaacaggga
    14401 ggtttgttat tccaccaaag catattgagc tttcaatgtg ttttgatagg ctaacgagcc
    14461 attgttcttt gaaaactgaa taaaagtaat cgaatacgtt tcgataagaa aagagccaac
    14521 ggaactaacc gccatgacct atcttataaa gatagcgagc gattcatgtt agtgatccga
    14581 gaagcaatct ttagcaggat tgcctgcaac gacattctta tcgtgataat gatactccca
    14641 tacagtcaat agtccgagcg tgataaaacc gtcgcaggca atgagtatgg ctacatgaga
    14701 accatgcagg ggtggaactc ccgtgagctt tgctaaagct gttcgattgc tggtaaaaca
    14761 acttttatga aatccaaata agtgatttgg aaaggaggat tttatgaagc agactgacat
    14821 tcctatttgg gaacgttata ccctaaccat tgaagaagcg tcaaaatatt ttcgtattgg
    14881 cgaaaacaag ctacgacgct tggcagagga aaataaaaat gcaaattggc tgattatgaa
    14941 tggcaatcgt attcagatta aacgaaaaca atttgaaaaa attatagata cattggacgc
    15001 aatctagcgt cgccaaaggg tcttgtatat gataaaatag tattaagtcg tatcaaggct
    15061 ctttccataa aggaaaggag caaatgccat gtcagaaaaa agacgtgaca ataaaggtcg
    15121 aatcttaaag actggagaga gccaacgaaa agacggaaga tacttataca aatatataga
    15181 ttcatttgga gaaccgcaat ttgtttactc gtggaaactt gtggctacag accgagtacc
    15241 agcaggaaag cgtgattgta tctcacttag agagaaaatc gcagagttac agaaagacat
    15301 tcatgatggt attgatgttg taggaaagaa aatgacactc tgccagcttt acgcaaaaca
    15361 gaacgctcaa agaccaaagg ttagaaaaaa cactgaaact ggacgcaaat atcttatgga
    15421 tattttgaag aaagacaagt taggtgtaag aagtattgac agtattaagc catcagacgc
    15481 taaagaatgg gctattagaa tgagtgaaaa tggttatgct tatcaaacca tcaataacta
    15541 caaacgttct ttaaaggctt cattctatat tgctatacaa gatgattgtg ttcggaagaa
    15601 tccatttgac tttcaactga aagcagttct tgatgatgat actgtcccta agaccgtact
    15661 aacagaagaa caggaagaaa aactgttagc ctttgcaaaa gctgataaaa cctacagcaa
    15721 aaattatgat gaaattctga tactcttaaa aacaggtctt cgtatttcag agtttggtgg
    15781 tttgacactt ccagatttag attttgagaa tcgtcttgtc aatatagacc atcagctatt
    15841 gagagatact gaaattgggt actacattga aacaccaaag accaaaagtg gcgaacgtca
    15901 agttcctatg gttgaagaag cctatcaagc atttaagcga gtgttagcga atcgaaagaa
    15961 tgataagcgt gttgagattg atggatatag tgatttcctc tttcttaata gaaagaacta
    16021 tccaaaagtg gcaagtgatt acaacggcat gatgaaaggt cttgttaaga aatacaataa
    16081 gtataacgag gataaattgc cacacatcac tccacatagt ttgcgacata cattctgtac
    16141 caactatgca aatgcaggaa tgaatccaaa ggcattacag tacattatgg gacatgctaa
    16201 tatagccatg acgctgaact attacgcaca tgcaacattc gattctgcaa tggcagaaat
    16261 gaaacgcttg aataaagaga agcaacagga gcgtcttgtt gcttagtagt acaaatgaat
    16321 ttactactta tttaccactt ctgacagcta agacatgagg aaatatgcaa agaaacgtga
    16381 agtatcttcc tacagtaaaa atactcgaaa gcacatagaa taaggcttta cgagcattta
    16441 agaaaatata aaaagataat tagaaattta tactttgttt ttattaaaaa gcagcaaact
    16501 ataaactagt aggttccaca ctaaatgtag ccccatactg ccccacaagt ccgatttata
    16561 gcgtaccatc cctaaaaaca ttcccaatga aacatataaa caccaagcta gaatggttcc
    16621 tggatgatga gctaaggcaa ataaaacact tgtcaaagca actcgaatat ctaattttct
    16681 aaccaagttc cataaaattt ctctatacaa aaattcttca accatactcg cattgattaa
    16741 gaacaataaa aatgaaaacc aaggaacttg atgttgaagc ccaattaagt ttgcttgatt
    16801 tgtgctttct tgagcatgaa tcagactaaa atatagactg ataatcagta ggctaacgaa
    16861 tccaatacca atccacttta tcctagtttc catattgacc ttggccactt gttttcgttg
    16921 accatacatc cataaaaaag aaatgagcga cgctccatag ataatctgta gtatagttaa
    16981 ctcaccgata caaagaaatt tca
//
LOCUS       DAYYQN010000042        16799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYYQN010000042 DAYYQN010000000
VERSION     DAYYQN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.042"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..522
                     /locus_tag="ACHBRQ_001440"
     CDS             <1..522
                     /locus_tag="ACHBRQ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9593090.1"
                     /translation="GGSGSGKSTLLNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNT
                     VGYVFQNYSLIDNKTVYDNLSLITKDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQR
                     VAIARMLMKPRKIILADEPTGALDGEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEV
                     DVIIDMKDIGYKV"
     gene            519..1019
                     /locus_tag="ACHBRQ_001441"
     CDS             519..1019
                     /locus_tag="ACHBRQ_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593091.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(1185..2531)
                     /gene="gor"
                     /locus_tag="ACHBRQ_001442"
     CDS             complement(1185..2531)
                     /gene="gor"
                     /locus_tag="ACHBRQ_001442"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9593092.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            2796..3995
                     /locus_tag="ACHBRQ_001443"
     CDS             2796..3995
                     /locus_tag="ACHBRQ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFM9593093.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            3979..4680
                     /locus_tag="ACHBRQ_001444"
     CDS             3979..4680
                     /locus_tag="ACHBRQ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9593094.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            4682..5941
                     /locus_tag="ACHBRQ_001445"
     CDS             4682..5941
                     /locus_tag="ACHBRQ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593095.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDISLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            6065..8062
                     /gene="metG"
                     /locus_tag="ACHBRQ_001446"
     CDS             6065..8062
                     /gene="metG"
                     /locus_tag="ACHBRQ_001446"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9593096.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHAHMEAVDYPRALEAVWTLISRTNKYIDETAPWILAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            8112..8303
                     /locus_tag="ACHBRQ_001447"
     CDS             8112..8303
                     /locus_tag="ACHBRQ_001447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593097.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSSKHCFEVVDETDEVS
                     NHTYGRATLTWFEEIFEEY"
     gene            8393..8713
                     /locus_tag="ACHBRQ_001448"
     CDS             8393..8713
                     /locus_tag="ACHBRQ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9593098.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            8717..9532
                     /locus_tag="ACHBRQ_001449"
     CDS             8717..9532
                     /locus_tag="ACHBRQ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593099.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFIS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            9607..10533
                     /locus_tag="ACHBRQ_001450"
     CDS             9607..10533
                     /locus_tag="ACHBRQ_001450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9593100.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDVLMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            10629..10865
                     /locus_tag="ACHBRQ_001451"
     CDS             10629..10865
                     /locus_tag="ACHBRQ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9593101.1"
                     /translation="MTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTIEQNGLALEMT
                     PYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            10858..11556
                     /locus_tag="ACHBRQ_001452"
     CDS             10858..11556
                     /locus_tag="ACHBRQ_001452"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9593102.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKSLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            11567..12046
                     /locus_tag="ACHBRQ_001453"
     CDS             11567..12046
                     /locus_tag="ACHBRQ_001453"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9593103.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQYRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            12054..12782
                     /locus_tag="ACHBRQ_001454"
     CDS             12054..12782
                     /locus_tag="ACHBRQ_001454"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9593104.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            12824..13693
                     /locus_tag="ACHBRQ_001455"
     CDS             12824..13693
                     /locus_tag="ACHBRQ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HFM9593105.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            13668..14108
                     /locus_tag="ACHBRQ_001456"
     CDS             13668..14108
                     /locus_tag="ACHBRQ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9593106.1"
                     /translation="MIPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLGDLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            14231..16777
                     /locus_tag="ACHBRQ_001457"
     CDS             14231..16777
                     /locus_tag="ACHBRQ_001457"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFM9593107.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
BASE COUNT         5135 a         2939 c         3744 g         4981 t
ORIGIN      
        1 ggaggttctg gctccggtaa aagtaccctt cttaatatta taggaagatt agaaaaaatt
       61 gacagtggga atgttttagt tgataaacaa gatatttgga agataaaaga aagaactttt
      121 tttaaaaata ctgttggata tgtatttcag aattattctt taatagataa caaaacagtg
      181 tatgataatc tgagccttat cactaaagac aaaaaaacaa taactgacgt acttgaaaaa
      241 gtaggactgt caagtgacta tttacaccaa aaaatatatg aattgtctgg agggcaggct
      301 caacgtgtag ctattgccag aatgttaatg aaaccacgta aaattatctt agcagatgaa
      361 cctacaggag ctttagatgg tgagattgga aaagaaatta ttcgtttact attgaatgaa
      421 acagctgaag ataaatatgt cattatagcg acacatgatc cagctgtcta taacgaagtt
      481 gatgtgataa ttgacatgaa agatataggg tataaggtat gaaaaagaaa atttatattg
      541 ctttgatctt tgtaactggg gtactcgcca ttttcttttt tggaaaacaa atgattacaa
      601 aagaaaatat caataagcca acggtagagt taacaatcta tacgctttct tctagtgata
      661 cagaaaaatg gaataaagtg agacaagtag aaacggaaga agctatatac ttcataactg
      721 taaaagaagt atcatcttca gaagaagtat tttcaaacat cattgcaaat ggagctgcta
      781 cggggttcgg agttcgtgaa gaagaagtga agaaattcaa taatggttta ggagacacca
      841 tagaggactc taagcataat aaactaattg aaatagaatt ttttactttt tctgatgatg
      901 gcgcgggatt tgtggtagca aactttgact acggtaaaga agagttgaat tctcagaaaa
      961 aagatataaa agaattatat aaaaaaatat atgaatcttt taaagaaaag aataaatgat
     1021 tgtattataa tccttgtatt ttgctatggt ttgttaattg atttttgcat atcttacttc
     1081 aacatactat aaaataaaac ataagaaaac gagtatctcc aactacggaa atactcgctt
     1141 tttgatattt agaagtgttt tttgctttgc ttcttttgtt tcgattaacg catggttaca
     1201 aattcttcag atgcagttgg gtgaattgcc acagttgcat caaagtcagc cttggttgct
     1261 cccattttga tagcaacggc aaatccttga atcatttcat caacgccgta gccaattcca
     1321 tgaagtccga caactttttc ttctgaacca gctgttatga gtttgaaacg ggattcttga
     1381 cggttgcgag tgcaagcaga gtacatagat gcaaagcttg atttataaac cttgatttgg
     1441 tcttgaccgt attctttaat agcttgctct tctgtcaaac caacagttcc gatagcaggg
     1501 tgtgaaaaga caacagttgg aatagttgag taatccattt ttgcagtagt ttttccgtta
     1561 aagagacgtt cagatagggt acgtccggcc ttgattgcaa ctggagtcag ttctttctcg
     1621 cccgttacat cacctagagc atagattccc tcaacaacag tattttggta ttcatccact
     1681 tggataaagc cacgttcgtt cagagtcact ccagcttttt caagttgcaa gcccttaacg
     1741 tttggacggc gacctgtagc ccagataact tggctagctg tgtgactagt accatcttcg
     1801 aaatgaatgg taatgccgtc agtagttttt tctaacttga cagggacttt gtgagtgtga
     1861 agtggtaagt ttgttctttc catttccttg accaaacctt caacgatgta ggaatcaaaa
     1921 ccacgtaaag gacgatcgcg gcgaacaaag agatctgtct tgacaccaaa agtgtggagt
     1981 acgccagcta attcaacggc gatataacca gcgcctagaa tggcaattga ctctggaagt
     2041 tcttcccagg caaatacatc atcagaagag ccacctagct cagcaccagg aatattagga
     2101 atacttggat gggcacctgt agcaatcacg atatgtttag cacgaatcag ttcaccattt
     2161 acgcttacag tatgagaatc tacaaattca gcatgacctt caatcaagtc tacaccgttg
     2221 cgtttaaaac taccatcata agaagaacga gcgcgatcaa tgtaggcttc acgattgcga
     2281 cgtagggttg caaagtcaaa gttaagatca gtagtcttaa agccgtagtc ttctccaaat
     2341 tgatggaaag tctcagcgat ttgcgccccg taccacatga tttttttagg aacacaaccg
     2401 acgttgacac aggttccacc taatttcttt tcctcaataa cggctgcttt ggctccatgt
     2461 tcaccagcac ggttcatggt agcgattcct ccgctacctc caccgatagc aatgatatca
     2521 tattctctca ttgaaaactc ctttgtaatc ttttaaatag tagagcgtct tttttgatag
     2581 ttatatttta ttcttttttt gatttgattg caaaaatctg ctacgcacaa aaaattgcaa
     2641 aaaagcttgc caaataagtc aagatagtgt attatatatc tagtacaata gaaaagcaaa
     2701 attccgaacg attgtttaat cctgaactgt attgtttgct ggagggaaat ttggtataat
     2761 agcaaagtat tgtaaaaaag aaaaggagtt tcattatgaa gaaaaagaat ggtaaagcta
     2821 aaaagtggca actgtatgca gcaatcggtg ctgcgagtgt agttgtattg ggtgctgggg
     2881 ggattttact ctttagacaa ccttctcaga ctgctctaaa agatgagcct actcatcttg
     2941 ttgttgccaa ggaaggaagc gtggcctcct ctgttttatt gtcagggaca gtaacagcaa
     3001 aaaatgaaca atatgtttat tttgatgcta gtaagggtga tttagatgaa atccttgttt
     3061 ctgtgggcga taaggtcagc gaagggcagg ctttagtcaa gtacagtagt tcagaagcgc
     3121 aggcggccta tgattcagct agtagagcag tagctaaggc agatcgtcat atcaatgaac
     3181 tcaatcaagc acgaaatgaa gccgcttcag ctccggctcc acagttacca gcgccagtag
     3241 gaggagaaga tgcaacggtg caaagcccaa ctccagtggc tggaaattct gttgcttcta
     3301 ttgacgctca attgggtgat gcccgtgatg cgcgtgcaga tgctgcagcg caattaagca
     3361 aggctcaaag tcaattggat gcaacaactg ttctcagtac cctagaggga actgtagtcg
     3421 aagtcaatag caatgtttct aaatctccaa caggggcgag ccaagttatg gttcatattg
     3481 tcagcaatga aaatttacaa gtcaagggag aattgtctga gtacaatcta gccaaccttt
     3541 ctgtaggtca agaagtaagc tttacttcta aagtgtatcc tgataaaaaa tggactggga
     3601 aattaagcta tatttctgac tatcctaaaa acaatggtga agcagctagt ccagcagccg
     3661 ggaataatac aggttctaaa tacccttata ctattgatgt gacaggcgag gttggtgatt
     3721 tgaaacaggg tttttctgtc aacattgagg ttaaaagcaa aactaaggct attcttgttc
     3781 ctgttagcag tctagtaatg gatgatagta aaaattatgt ctggattgtg gatgaacaac
     3841 aaaaggctaa aaaagttgag gtttcattgg gaaatgctga cgcagaaaat caagaaatca
     3901 cttctggttt aacgaatggt gctaaggtca tcagtaatcc aacatcttcc ttggaagaag
     3961 gaaaagaggt gaaggctgat gaagcaacta attagtctaa aaaatatctt cagaagctac
     4021 cgtaatggtg accaagaact gcaggttctc aaaaatatca atctagaagt gaatgagggt
     4081 gaatttgtag ccatcatggg accatctggg tctggtaagt ccactctgat gaatacgatt
     4141 ggcatgttgg atacaccaac cagtggagaa tattatcttg aaggtcaaga agtggctggg
     4201 cttggtgaaa aacaactggc taaggtccgt aaccaacaaa tcggttttgt ctttcagcag
     4261 ttctttcttc tatcgaagct caatgctctg caaaatgtag aattgccctt gatttacgca
     4321 ggagtttcgt cttcaaaacg tcgcaagttg gctgaggaat atttagacaa ggttgaattg
     4381 acagaacgta gtcaccattt accttcagaa ttatctggtg gtcaaaagca acgtgtagcc
     4441 attgcgcgtg ccttggtaaa caatccttct attatcctag cggatgaacc gacaggagcc
     4501 ttggatacca aaacaggtaa ccaaattatg caattattgg ttgatttgaa taaagaagga
     4561 aaaaccatta tcatggtaac gcatgagcct gagattgctg cctatgctaa acgtcagatt
     4621 gtcattcggg atggggtcat ttcgtctgac agtgctcagt taggaaagga ggaaaactaa
     4681 gatgcagaat ctgaaatttg ccttttcatc tatcatggct cacaagatgc gttctttgct
     4741 tactatgatt gggattatta tcggtgtttc atcagttgtt gtgattatgg ctttgggtga
     4801 ttccctatct cgtcaagtca ataaagatat gactaaatct cagaaaaata ttagcgtctt
     4861 tttctctcct aaaaaaagta aagatggttc ttttactcag aaacaatcat cttttacggt
     4921 ttctggaaag gaagaggaaa ttcctgttga accgccaaaa ccgcaagaat cctgggtcca
     4981 agaggcagct aaactgaagg gagtggatag ttactatgta accaattcaa cgaatgccat
     5041 cttgacctat caagataaaa aggttgagaa tgctaatttg acaggtggaa acagaactta
     5101 catggacgct gttaagaatg aaattattgc aggtcgtagt ctgagagagc aagatttcaa
     5161 agagtttgca agtgttattt tgctagatga ggaattgtcc attagtttat ttgaatctcc
     5221 tcaagaggct attaacaagg ttgtagaagt caatggattt agttaccggg tcattggggt
     5281 ttatactagt ccggaggcta aaaggtcaaa aatatatggg tttggtggct tgcctattac
     5341 taccaatatc tcccttgctg cgaattttaa tatagatgaa atagctagta ttgtctttcg
     5401 agtgaatgat atcagtttaa ccccaactct gggtccagaa ctggcacgaa aaatgacaga
     5461 gcttgcaggc ttacaacagg gagaatacca ggtggcagat gagtccgttg tatttgcaga
     5521 aattcaacaa tcgtttagtt ttatgacgac gattattagt tccatcgcag ggatttctct
     5581 ctttgttgga ggaactggtg tcatgaacat catgctggtt tcggtgacag agcgcactcg
     5641 tgagattggt cttcgtaagg ctttgggcgc aacacgtgcc aatattttaa ttcagttttt
     5701 gattgaatcc atgattttga ccttgttagg tggcttaatt ggcttgacaa ttgcaagtgg
     5761 tttaactgcc ttagcaggtt tgttactgca aggtttaata gaaggtatag aagttggagt
     5821 atcaatccca gttgccctat ttagtcttgc agtttcggct agtgttggta tgatttttgg
     5881 agtcttgcca gccaacaagg catcgaaact tgatccaatt gaagcccttc gttatgaatg
     5941 agatcaacaa gatggacact cgtctatctt gtttttgtat ggatttccct atcgaaattc
     6001 attaaaaatg tgatataatg aagtgttaga aaaacaggtt tcatttgcct ggaaaggaaa
     6061 aattatgtct gaaaaaaatt tttatattac aacgccgatt tactatccat ctgggaaact
     6121 tcatatcggt tctgcctaca caactatcgc atgtgatgtc ctagcacgtt acaaacgcct
     6181 gatgggctac gatgtatttt atctgacagg tcttgatgaa catggtcaga aaatccagca
     6241 gaaagcggaa gaagctggta ttacacctca agcctatgtt gatggaatgg cggttggagt
     6301 taaagaactc tggcaattac tagatatctc atacgataaa tttatccgta caactgatga
     6361 ctaccatgaa aaagttgttg cacaggtctt tgaacgctta cttgctcaag atgatatcta
     6421 cttgggtgaa tactctggtt ggtattcagt atcagacgaa gaattcttta cagaaagcca
     6481 gctggcagaa gttttccgtg atgaagctgg aaatgtgact ggtggtattg ctccatcagg
     6541 tcatgaggtt gaatgggttt ctgaagaatc atacttcctt cgccttagca aataccaaga
     6601 ccgtttggtc gaatttttca aagctcatcc tgaatttatc acgccagagg gtcgccttaa
     6661 tgaaatgcta cgcaacttca tcgagccagg tttggaagat ttggcggtat ctcgtacaac
     6721 ctttacatgg ggagtgcctg tcccatcaaa tccaaaacac gttgtctacg tttggattga
     6781 tgcccttctt aactatgcga cagctcttgg ctacgctcaa gacgaacatg gtaactttga
     6841 caagttctgg aatggaacag tcttccatat ggtaggaaaa gacatccttc gcttccactc
     6901 tatctactgg ccaatccttc ttatgatgtt ggatgttaaa ttacctgatc gtttgattgc
     6961 ccatggttgg tttgtcatga aagacggaaa aatgtctaag tcaaaaggga atgtcgttta
     7021 ccctgaaatg ttggtagagc gttatggact agatccactt cgttactacc tcatgcgtaa
     7081 ccttccagtt ggttcagacg gaacctttac tcctgaagac tatgtcggcc gtatcaacta
     7141 tgaattggct aatgaccttg ggaacctcct taaccgtacg gtttccatga ttaataagta
     7201 ctttgatgga caaatccctg cctatgtaga aggtgtgact gaatttgacc atgttcttgc
     7261 tgaggttgca gaacaatcaa tcgcagactt ccatgcacac atggaagcag ttgactatcc
     7321 acgtgcgctt gaagcagtct ggactctgat ctctcgtacc aataaataca tcgatgagac
     7381 cgcaccatgg atcttggcca aggatgaagc tcttcgtgac caattggcaa gtgtcatgag
     7441 ccacttggca gccagcattc gtgtagttgc tcacttgatt gaaccattta tgatggaaac
     7501 tagtcgtgca gttttgactc aacttggttt ggaagaagtt tctagtcttg aaaacttgag
     7561 tttggctgac ttcccagcag atgtgactgt agttgccaaa ggaacaccta tctttccacg
     7621 tctaaatatg gaagaagaaa tcgcctatat caaggaacaa atggaaggca ataaaccagc
     7681 agtcgaaaaa gaatggaatc cggacgaagt tgagctcaaa ctaaacaagg atgaaatcaa
     7741 gtttgaagac tttgacaagg ttgaaatccg tgtggcagaa gtcaaagaag tgtctaaagt
     7801 agaaggttca gataagttgc ttcaattccg cttggatgct ggtgatggag aagatcgtca
     7861 gattctttca ggaattgcaa aatactatcc aaatgaacaa gaattggtcg gcaagaaagt
     7921 tcaaatcgtt gctaacctca aaccacgtaa aatgatgaaa aaatatgtca gccagggtat
     7981 gattctatca gctgaacatg atggcaaatt aacccttctc acagttgatc cagctgtacc
     8041 aaatggaagt gtgattgggt aaaagtagac aggactagtt cagactagtt ctgttttttt
     8101 atttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt tgctcaaagc
     8161 actgctttga ggttgtagat gaaactgacg aagtcagctc aaaacactgt tttgaggttg
     8221 tagatgaaac tgacgaagtc agtaaccata catacggtag ggcgacgctg acgtggtttg
     8281 aagagatttt cgaagagtat taactattat gctttacaaa ctagtgtaga agtaatatac
     8341 tataggtgaa gttactagta ggtattacaa aatagattag aagggagaag tgatgaaaga
     8401 aactcagcta ttaaaaggtg ttcttgaagg ttgtgtcttg gatatgattg gtcaaaaaga
     8461 gcggtatggt tatgagttgg ttcagacttt gcgagaggct ggatttgata ctatcgttcc
     8521 aggaactatt tatcctttgt tgcaaaagtt agaaaaaaat caatggataa gaggcgacat
     8581 gcgcccgtcg ccagatggtc cagatcggaa gtatttttca ttaatgaaag aaggagaaga
     8641 gcgtgtctca gtcttttggc aacaatggga cgatttgagt caaaaagtag aagggattaa
     8701 gaatgggggt taaaccatga agaaaatgaa gtattacgaa gaaacaagcg ctttgctaca
     8761 tgagttttct gaggagaatc aaaagtattt tgaggagttg tgggaaagtt ttaatcttgc
     8821 tggatttctc tatgatgaag actatctcag agagcagatc tatttgatga tgctagattt
     8881 ctcagaagca gaacgagatg gcatgagtgc agaggattat ctaggtaaga atcctaaaaa
     8941 aataatgaaa gagattctca agggagcacc tcgcagttct atcaaagagt cccttttgac
     9001 gccaattctt gtcctggcgg tattacgtta ttatcaacta ctaagtgatt tttctaaagg
     9061 tcctctctta acagtcaatt tgctcacatt tttagggcaa cttcttattt ttctgattgg
     9121 atttggactt gtggccacaa ttttacgaag aagtttagtc caagattctc ctaaaatgaa
     9181 aattggcact tacattgttg ttgggactat agttcttcta gttgttttag gatatgtagg
     9241 aatggcaagc ttcatacaag aaggagcctt ttatattccg gctccctggg atagtttgtc
     9301 tatctttacg atttcgctag ttatcggtat ttggaattgg aaagaagcgg tctttcgtcc
     9361 atttatcagt atgattattg cccatcttgt ggtgggttct ctgctccgtt attatgagtg
     9421 gatgggaatt tcaaatgttt tccttacaaa agttattcct ttagctgtcc tctttattgg
     9481 aatctttgtc ttgttccgtg ggtttaagaa gataaaatgg agtgaagtat agtcaaaaag
     9541 tcgttgcaaa gcggtttttt gttataatga agggaagaat gttagaaatt aaaggagaaa
     9601 aatttgatga gatacataac tcttggtcaa gatgacaaag aattatcaga aattgttctc
     9661 ggaatgatga gaataaaaga taaatctgta aaagaagttg aagagcttgt agaaacagca
     9721 ctttctgttg gaatcaatgc cttcgacttg gctgatattt atggtcgtgg tcgttgtgaa
     9781 gaactgttag gtcttgtcct aaaaaatcgt ccagatttaa gagaaaagat gtggattcag
     9841 tccaaatgtg gcattcgcat tgaagaattt acctattttg atttttctaa ggactatatt
     9901 ataaaatcag tagacggtat tttgcaaaga ttgaagattg atcatctaga tagcttgctc
     9961 cttcatcgac cagatgtttt gatggaatct gaccaagtag cagaagcctt taatctcctt
    10021 tataaacaag gtaaagttcg agattttgga gtttctaatc aaaatcctat gatgatggag
    10081 ttgcttaaaa aagatgtcaa gcagccgtta gctgttaatc agctacaatt gagtgcggct
    10141 tttactccag gatttgaatc agcttttcat gttaatatgg aagatagtca agcagctatg
    10201 cgagatggca gcatttttga atattgccaa ttacacgatg tggtcattca agcatggtct
    10261 gtcttacaat tcgggtattt taaagggaat tttgttggaa atgagaaatt tcaagcttta
    10321 aatcaagtac ttgatcgttt agctattaaa tatggagtaa cctcttcaac tattgccatt
    10381 tcttggatat tgcgttatcc agcaaaaatg caggcagtcg taggtacaac aaatcctaag
    10441 cacttgagag aagttagccg agcggcaaac tttagcttaa caagaaaaga atggtatgaa
    10501 atttatcttg ctgcagggaa taatttgccg taagtagtag caggtttgaa taactcacat
    10561 tcaaaaagcc gtataaagcg gttttttgct ataattaaat gaagaatgta gaattaaagg
    10621 agaaaaacat gacatttgaa gagatcctgc ctgggttaaa ggctaagaga aaatatgtac
    10681 gaactggttg gggaggtgcg gaaaactatg tccaactctt tgataccatc gagcaaaatg
    10741 ggcttgcact tgaaatgaca ccttatttcc taatcaacgt ttctggcgaa ggagaaggtt
    10801 tttccatgtg gagcccgaca gtttgtgatg ttttggcaac ggattgggta gaagtgcatg
    10861 actaaacgtg tactcattac aggagtgagt tcagggatcg gattggctca agctcgcctc
    10921 tttttagaga agggctatca agtttatgga gttgaccaag gtgaaaagtc actcttagag
    10981 ggtgattttc actttttaca gagagatttg accttggact tggaacctat ttttgactgg
    11041 tgccctcagg tggatgtttt gtgtaatact gctggagttt tggatgatta caaaccactg
    11101 ttggaacaaa cggcgcagga cattcaagag atttttgaaa tcaactacat tattccagta
    11161 gagttgactc ggtattattt gacacaaatg ctggaaaata aaaagggaat catcatcaat
    11221 atgtgttcca ttgcttctag cctagcaggt ggaggtggtc acgcctatac ttcatccaag
    11281 catgccttgg ctggcttcac caagcagttg gctctagact atgctgaagc tgggattcag
    11341 gtctttggta tcgctccagg agcagtcaag acagctatga ccgctgcgga ttttgaacca
    11401 ggtggtttgg ctgactgggt ggctagtgaa accccaatca agcgctggat tgagccagag
    11461 gaaatagcag agcttagtct ttttttagca agtggaaaag cgagcgccat gcaaggacaa
    11521 atcttgacaa tagatggtgg ctggtctttg aagtaggagg agttggatgg aaatcaaaaa
    11581 acactttgga gtctacgctg tttgcttaga aaatgggaag ttactctgca ttgaaaaaac
    11641 gagaggccct tatcaatatc ggtatgatct acctggaggc agtcagcaac ttggtgaagg
    11701 actgacggaa acgctgacta gagaagttat ggaagagacg ggatttactg ttagaagcta
    11761 ctctaatcct cgaatctacg atgttttcgt tagggaagag ttaaaaaatt ttatggttca
    11821 ccatgtcatg gccttgtatg atgttgaaat gaatgagagt gcacctcaag ttacgacttc
    11881 ggaagctgtg tctgatggtg cgaatgattc acttggatat atttggatgg atattcaaga
    11941 gattacagaa gaaaatgcat cgccattagt cttgaaggtt aagtctgaat tattaggatt
    12001 tccagaactg gacaagactt cttacatgaa ttggaaggtg aagtagaggg ataatggaac
    12061 tatttaaaac atggaagaaa aatatggttc tctatggtct taaatctcaa attggaactg
    12121 tctaccgaaa caatgatagg acaacaagct tttatgatgt tgggaatttt ctatacctag
    12181 caggggagtt ggattccaga ttttgggaag attttgttag aaaatatggt ttagattata
    12241 agattattat ttcagaaaat actaattggc aagattttct gcatcgaaaa gtggggctaa
    12301 attcttttac tcgctattct tttaaagata aggcaaattt tcaagttgaa tttttaaata
    12361 atctagttac tcatttagag gaaggttaca atattgtgcc tattgataat catatttata
    12421 actgcttttc tacggaagaa tggtcacagg atttacaggg ggattttgag tcctatcagg
    12481 attttgtttt aaaaggtgga tttggctttg tgattcttaa aaataatgaa ctgattgctg
    12541 ggatttcctc agggttagtt taccgtaaag cagttgaagt ggaagttgca actagaccaa
    12601 acgaacaagg aaatggattt gctaaaaagc ttggtgctgc aatgattcta gagagtttaa
    12661 atagagatat gtttccactt tgggatgctc ataatgaggc ttccaaaaaa gtagcagaat
    12721 ttttaggata tgagttatct gaaccttatg aagcttttga actagaggaa attttgatat
    12781 aattatatat gatattgtat cttttaagac gaggtgaatg gctatgagaa atcaacttgc
    12841 ccttagtggc gaaaaaattc ttgaaaaaat ttatcctcaa ctatttcacc acgtcggcat
    12901 gattcgtggg gaatacttat tgagagagct taatcaaaac atcatattag caagttgtca
    12961 gcaatttgta aaagattatc ttgatactat ttgctccttt tacttagata aggaagtttg
    13021 gtatcgtttt tctgaattaa cgaatacaga agccaattgt ttagcgggga ctagagagtt
    13081 ttttgacgaa gggcatccct tgtttggcta tagaggaacc aggcgtttac tggcgtgttt
    13141 ggatgaattt caggctgaag cacatgtcgt tacagaagtt tatcaaacca atcccaatct
    13201 gtctcttatt tttccttttg tcaatgatgc cgaccaatta aaacaagcta ttacagtatt
    13261 gcgtcagtat ggctttactg ggaaagtcgg aacgatgatt gaattaccgt cagcgtattt
    13321 tgacttatct agtatactgg aaacgggcat ttcaaagatt gtagttggaa tgaatgattt
    13381 gacttccttt gtttttgcga ctatgagaaa cagtcaatgg catgatatgg aaagtccaat
    13441 aatgttagat atgctaagag atatgcagga taaagcaaga aagaacaaga ttaattttgc
    13501 tgtagcaggc tatctgaata cttctttcat acaaaaaatg aatcagttgg gtatcaagtg
    13561 cattatccac tacagttcta ttccagagat ttttgattta gagattgacc atccagatca
    13621 tcttaaacac ataaaagaag aaagtaaaaa attacaaagg agtacccatg ataccgcaag
    13681 aaatgtggaa taaatacaag caaatcaatc cttctatcgg agatgaaatc gatgcttggg
    13741 cttttggagt tgaaccagac cttttagggg atttggtgtt taaaggagaa aagacagcaa
    13801 cagcatcagc ctatgacctc tatgtactag aagacgaacc acttccccaa gtagggacct
    13861 tcgatgtcat tttagatagt caaaatcagg ctgtctgcat tgtcgaaatt acaaaggttt
    13921 ctgttgaact cttcaatcaa gtttctgcgc aacatgcctt taaggaaggt gagggagaca
    13981 aatcacttgc ctattggcgc caggttcatg aggacttttt cacagactgt ttgggtgaag
    14041 tagggctgac ttttacacct gaaagcaagg ttgttttaga agaatttcgc aaggtctacc
    14101 cactgtagac tattagaagg aagaaagttt tggaaatcgc tgtccaatcc ttttttctca
    14161 agcaaaatat gatataataa gtttgtttga agaagagcag cagctcttaa acttagaata
    14221 ggagaaaact atgcaagcag ttgaacattt tattaagcaa tttgttcctg aacattatga
    14281 tttattttta gatttgagtc gtgagaccaa gactttttct gggaaagtga ccatcactgg
    14341 tcaagcacag agtgaccgca tctccctcca ccaaaaagac ttggaaatca cctctgtaga
    14401 agttgcaggt caagctcgtc catttacagt tgaccatgac aatgaagccc ttcatatcga
    14461 attggctgag gctggtcaag ttgaattggt tcttgccttt tctggtaaaa ttacagacaa
    14521 catgacaggg atttaccctt cttattatac agttgatgga gtcaagaagg aggtcttgtc
    14581 tactcagttc gagagccatt ttgcgcgcga agctttccca tgtgtggatg agcctgaagc
    14641 caaagcaact tttgacctct ctcttcgctt tgaccaagca gaaggtgaat tggccttgtc
    14701 aaacatgcca gaaatcgatg ttgaaaaccg taaggaaaca ggtatctgga agtttgagac
    14761 aacacctcgc atgtcttctt acttgttggc ctttgttgct ggtgatttgc aaggggtgac
    14821 cgctaaaact aaaaatggta ccctggtagg tgtctactca accaaagcac atccactttc
    14881 aaatcttgat ttctcactgg atatcgctgt tcgctctatc gagttttacg aagattacta
    14941 tggagttaag tacccaattc ctcaatctct ccacatcgcc cttcctgact tctcagctgg
    15001 tgctatggaa aactggggtc ttgtgaccta ccgtgaagtt tacttggttg tcgatgagaa
    15061 ctctacattt gctagccgtc aacaagttgc ccttgttgtg gcccatgaat tggctcacca
    15121 atggtttggg aacctcgtga ctatgaaatg gtgggatgac ctttggctca atgaaagttt
    15181 cgctaatatg atggaatacg tctgtgtgga taccatcgaa ccaagctgga atatctttga
    15241 agatttccaa acaggtggag tacctcttgc tcttgaacgt gacgctactg atggcgttca
    15301 gtctgtccac gtcgaagtta aacatccaga tgaaatcaat acactctttg acggcgctat
    15361 cgtctatgcc aaaggaagcc gtctcatgca catgcttcgc cgttggctag gagatgctga
    15421 ttttgctaaa ggtttgcatg cctactttga aaaacaccaa tacagcaaca ccattggtag
    15481 tgacctttgg gatgcccttg gtcaagcgtc aggacgtgat gtcgcagcct tcatggattc
    15541 ttggttggaa cagcctggtt acccagttct tactgtcaaa gttgaaaatg atgtcttgaa
    15601 gatttcacaa aaacaattct tcatcggtga gaacgaagac aagaaccgtc tctgggtggt
    15661 gcccctcaat agcaactgga aaggcttgcc tgatacactc gaaactgaaa gtatcgaaat
    15721 ccctggctac gcagctcttc ttgctgaaaa tgaaggagct cttcgtctca acactgaaaa
    15781 tactgcccac tatattaccg actatcaagg agacttgtta gaagctgttc ttgctgagct
    15841 agagacactt gataacacaa gcaaactgca aattgttcaa gaacgtcgtt tgttggctga
    15901 ggcagggcac atttcttatg cagacttgct tccagttctt gataaacttg ctaaggaaga
    15961 gtcttatctg gtggtttcag ctgtttctca agtgatttca gcccttgagc gctttattga
    16021 tgaaggaacg gatgctgaaa cagcctttaa aggactggtt gctaaattgg ctcgtcataa
    16081 ctatgaccgt cttggttttg aagctaaaga cggagaatca gatgaggatg aattggttcg
    16141 tcagctggct gtttcgatga tgattcgctc aaatgatgca gaagctagtc aagtcgctag
    16201 ccaaatcttc gcaacacaca aggagaatct tgcaggactc ccagcagcta ttcgctcaca
    16261 agttctgatt aatgagatga aacatcatga gactaaagac ttgttagcac tttatcttga
    16321 tacttatact cacgcaacag atgctgtctt taaacgccag ttggcagcag ctcttgcata
    16381 cagtacagat gcggataata tccaaaacct gattacttct tggaaggaca aatttgtggt
    16441 caaaccacag gacttgtctg cttggtatta ccaattccta gctcatcaag caactcagaa
    16501 aacagcttgg tcttgggcgc gtgaaaactg ggcttggatt aaggcagccc ttggaggaga
    16561 tatgagcttt gatagctttg ttatccttcc tgctcatgta tttaagactc agcaacgctt
    16621 ggcggaatac aaggagttct ttgagccgca gctttctgac cttgctctta gccgtaacat
    16681 cggtatggga atcaaggaaa ttgcagcacg tgttgacttg attagccgtg aaaaagctgc
    16741 agtcgaagca gttgttcttc aatacggaaa tgcataaata agcctaaaat aaaaagaaa
//
LOCUS       DAYYQN010000043        16727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYYQN010000043 DAYYQN010000000
VERSION     DAYYQN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.043"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..1069)
                     /locus_tag="ACHBRQ_001458"
     CDS             complement(<1..1069)
                     /locus_tag="ACHBRQ_001458"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9593108.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVG"
     gene            1127..2293
                     /locus_tag="ACHBRQ_001459"
     CDS             1127..2293
                     /locus_tag="ACHBRQ_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HFM9593109.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            2432..2758
                     /locus_tag="ACHBRQ_001460"
     CDS             2432..2758
                     /locus_tag="ACHBRQ_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9593110.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2745..3338
                     /locus_tag="ACHBRQ_001461"
     CDS             2745..3338
                     /locus_tag="ACHBRQ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9593111.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3331..4242
                     /locus_tag="ACHBRQ_001462"
     CDS             3331..4242
                     /locus_tag="ACHBRQ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HFM9593112.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4304..5368
                     /locus_tag="ACHBRQ_001463"
     CDS             4304..5368
                     /locus_tag="ACHBRQ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HFM9593113.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5546..6406)
                     /locus_tag="ACHBRQ_001464"
     CDS             complement(5546..6406)
                     /locus_tag="ACHBRQ_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HFM9593114.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(6528..7514)
                     /locus_tag="ACHBRQ_001465"
     CDS             complement(6528..7514)
                     /locus_tag="ACHBRQ_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9593115.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7786..9261)
                     /locus_tag="ACHBRQ_001466"
     CDS             complement(7786..9261)
                     /locus_tag="ACHBRQ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9593116.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDDYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            9320..9445
                     /locus_tag="ACHBRQ_001467"
     CDS             9320..9445
                     /locus_tag="ACHBRQ_001467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593117.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSYIYLRQGDVDAV"
     gene            complement(9519..10442)
                     /locus_tag="ACHBRQ_001468"
     CDS             complement(9519..10442)
                     /locus_tag="ACHBRQ_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9593118.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10456..11385)
                     /locus_tag="ACHBRQ_001469"
     CDS             complement(10456..11385)
                     /locus_tag="ACHBRQ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593119.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11704..12054)
                     /locus_tag="ACHBRQ_001470"
     CDS             complement(11704..12054)
                     /locus_tag="ACHBRQ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593120.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
     gene            complement(12480..12635)
                     /locus_tag="ACHBRQ_001471"
                     /pseudo
     CDS             complement(12480..12635)
                     /locus_tag="ACHBRQ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            complement(12820..13431)
                     /gene="rpsD"
                     /locus_tag="ACHBRQ_001472"
     CDS             complement(12820..13431)
                     /gene="rpsD"
                     /locus_tag="ACHBRQ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9593121.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLARLPERDEINPEINEALVVEFYNKML"
     gene            complement(13626..14678)
                     /locus_tag="ACHBRQ_001473"
     CDS             complement(13626..14678)
                     /locus_tag="ACHBRQ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9593122.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(14675..15373)
                     /locus_tag="ACHBRQ_001474"
     CDS             complement(14675..15373)
                     /locus_tag="ACHBRQ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9593123.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(15540..>16727)
                     /gene="pavB"
                     /locus_tag="ACHBRQ_001475"
     CDS             complement(15540..>16727)
                     /gene="pavB"
                     /locus_tag="ACHBRQ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HFM9593124.1"
                     /translation="DSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLK
                     ASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGT
                     QTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVKDSIDVPAA
                     YLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLG
                     ENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNI
                     VATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNT
                     MAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKK
                     EEN"
BASE COUNT         4877 a         3588 c         3138 g         5124 t
ORIGIN      
        1 aacctacaat cgtcccaagg ccataagcaa agtgaatgga aaataaaaga aagggcatca
       61 caattagaaa tccattttta tgttttaata aagtcagcaa agtgagtaat gacaaaagta
      121 gaaaataggc acctaatagt aaagttatga atacgaatgt gatcggtaac aatgctagac
      181 taaacacaag actcaaaaca aataaacaag gaacatagtg aaataatgat aaacacttaa
      241 actgaacatg acttgtcaag ccaatccaca aaccatttga atacttttga tgcagcattt
      301 tcttgaatgt tggtcgaata tactgataag atagaatact tgggctatag cggattttat
      361 aaccatattc tcgaattcta taatgaatat cattatcttc agttcggcca agttgctcat
      421 ttactaaacc aaccttctgg aaaacctctc gtttatacat tccatgaaaa atagaagaaa
      481 catatctatc ctcagaacta tttcgataat tggcaatgct actgccaaac atattttcct
      541 caacaagatg caaggtctct gcccattttc cttttccttc gacaatcgtc ggtctaggcc
      601 ccccacagac aaattcacct tgttgaataa tagccacatt gttcattaca aaagtctcag
      661 taacttttga atgagcatca atttttaaaa taaggtcccc tacagaatgt ttaactccca
      721 ggttaaaacc actagcttga tttttcttag gattgttata caatctaatt gagttaaact
      781 ctgtatcttc ctttataaat tgctgaatga tagctgtggt cccatctgtg gacatagcat
      841 ttataaatag aatttcaata ttctctttag gataggtttg attttttaag tcttcaatta
      901 gaccaggcag atatttttct tcattgtatg cactgattac aatggatacg agaagacttt
      961 caacattttg agtcactact actctcctga atttttcctt ccaccattct atcataattt
     1021 tcaagacttt cccattttta tcttgaaagc cagaaacctt acttgacatt atagtgaaaa
     1081 agccttaaaa taagctgtta ttaaaatcag ctagaagagg tattatatga aaaagcaatc
     1141 actctttttt gttccaggta ttatcctgat tggtgtttcc ttgcgaactc cttttactgt
     1201 tttacccatt attttgggaa atatttcgca aggtctggag gtagaagtta gttcgcttgg
     1261 tgtcttgacc agcctgcctc tccttatgtt taccctcttt tcaccatttt ctacccaact
     1321 ggctcagaaa atcggcttgg agcatctctt cacctacagc ctcttcttct tgaccatcgg
     1381 ctcacttatt cgactaatca atctgcccct gctctatcta ggaaccttga tggttggggc
     1441 aagtgtcgca gtcatcaatg tcctgcttcc tagtcttatc caagccaatc aaccaaagaa
     1501 aattggtttt ctgaccacct tatatgtaac gtctatgggg attgcaacgg ctctggcttc
     1561 ctatctagct gtgcccatta cacaagccag ttcttggaaa ggacttatcc ttctcctcac
     1621 gttactctgt ctagcaactt ttttggtctg gctcccaaat caccgctata atcatagact
     1681 agctccacaa accaaacaaa aaagtcaaat aaaggtcatg cgtaataaac aggtttgggc
     1741 aattattatc ttttcaggtt ttcaatcctt gatcttttac accgtcatga cctggttacc
     1801 taccatgtct atccatgcag gtctatccag tcacgaagct ggcttgctga cttctatctt
     1861 atctctgatt agcattcctt tttcaatgac catcccaagc ctgacaacca gtttatctac
     1921 tcgcaaccgt cagctcatgc tcactctggt ttcactagct ggtgtggtcg gcatttccat
     1981 gctctttttc ccaatcaata atttcattta ctggcttacc atccatctcc tcatcggaac
     2041 cgcaaccagc gccctcttcc cttatctcat ggtcaacttt tcactcaaga caagcgcccc
     2101 tgaaaagaca gcccaattgt ccggcctatc tcaaacagga ggctatatcc tagcagcctt
     2161 tgggccaacc ctctttggtt acagttttga cctgttccac tcttgggtac cagctgtagc
     2221 tgccctcttg ctcatctata tcctgatgac tgtggccctc tttacagtgg acagagcgga
     2281 taagatcctt taaacttcta gttttagcta gaagtttttt atatataaaa attttacaca
     2341 tttctcttga cagggctttc ttttagatgt acaatgtatg tgtagaaaaa ttatatataa
     2401 aaatcttaca cattagaaaa ggaggtttcc catgtacttt ccaacatcct ctgccttgat
     2461 tgaatttctc atcttggctg tactggagca gggtgattct tatggttatg agattagcca
     2521 aaccattaag ctgatcgcta atatcaaaga atccacactc tatcccattc tcaaaaaatt
     2581 ggaaggcaat agctttctga caacctattc tagagagttc caaggtcgca tgcgcaaata
     2641 ctactccttg acaaacggtg gtatagagca gctcttgacc ctaaaagatg aatgggcact
     2701 ctatacagac accatcaatg gcatcataga agggagtatc cgccatgaca agaactgaat
     2761 acctgactca gctagaactc tatctcaaga aactacctga agctgaccgt atcgaagcca
     2821 tggactattt cagagagctc tttgacgatg ctggagtcga aggagaagaa gaactcatcg
     2881 ctagtttggg aactcccaaa gaagcggccc acgaagttct atccaatctt ctcgataaaa
     2941 aaatcaatga agcacccgct caaaaaaata accgacaaat tttacatatc gccttgttag
     3001 ccctccttgc agcacctatc ggcattcctc tgggaatcgc catcctcgtg accctgttcg
     3061 caatccttgt agccgctttg actgtcattc tggctttctt tgcagtttcc atactgggta
     3121 tcatcggcgg attcctattt ttagttgaaa gtttcactat cctcgcccaa gccaaatcag
     3181 cctttatctt gatttttggt tctggtttac tggctatcgg tgcttcttcg ctagttttac
     3241 ttggcatttc ctatgtagct cgcttcttcg gtctactcat tgttcgtctg gtacaatttg
     3301 ttcttaaaaa aggaaagaga ggtaatcagc atgcgtaaat ggacaaaagg atttctcatc
     3361 tttggtgtgg tgactaccgt tatcggcttt atcctgcttt ttgtaggtat ccaatctgac
     3421 gggattaaga gcctactttc catgtccaaa gaacctgtct atgatagccg tacggaaaag
     3481 ctaacctttg gcaaggaagt cgaaaaccta gaaattactc tccaccaaca cacgctcacc
     3541 atcacagact ctttcgatga tcaaatccac atttcttacc atccatctct ttctgctcac
     3601 catgatttta tcaccaatca gaacgataga actctgagtc tcactgataa gaaactgtct
     3661 gaaactccgt ttctctcttc tggaattggt gggattcttc atatcgcaag tagctactct
     3721 agtcgttttg aagaagttat tctccgacta ccaaaaggga gaactctaaa agggatcaac
     3781 atctcagcca atcgcggaca aaccaccatc ataaatgcta gccttgaaaa tgcgaccctc
     3841 aatacaaaca gctatatcct ccgaattgaa ggaagtcgta tcaaaaacag taaactcaca
     3901 acgcccaata tcgttaatat ctttgataca gttcttacag atagtcagct agagtcaaca
     3961 gataatcact tccacgctga aaatatccaa gtccatggta aggttgaact gactgccaaa
     4021 gattatctca gaatcatcct agaccagaaa gaaagccaac gaattaactg ggacatctca
     4081 agtaactacg gttctatctt ccaattcaca agagaaaagc ctgaatcaag aggtacggaa
     4141 ttaagcaacc cttacaaaac tgaaaaaacc gatgtcaagg atcaactcat tgcgagatct
     4201 gatgataata ttgatctaat atccacacca agcagacgtt gacaaaaacg cttacatctg
     4261 ctactatgga agcatattta tctaaccaaa aggaggttct accatgaaac aagaatggtt
     4321 tgaaagtaat gattttgtaa aaacaacaag caagaacaag cctgaagagc aagctcaaga
     4381 ggttgcagac aaggctgaag aaacgatagc cgatttcgat acaccaattg gaaaaaatac
     4441 tcaagtagag gaggaagtct ctcaagctga agtcgaactg gaaagccagc aagaagagaa
     4501 aattgaaact cctgaagtcg gtgaagcgag aacagaaata gaagaaaaga aggcatctaa
     4561 ttctactgaa gaagagtcag acctttctaa agaaacagaa aaagtcacta tagctgaaga
     4621 gagccaagaa gctcttcctc agcaaaaagc aaccacgaaa gagccgcttc ttatcagtaa
     4681 atctttagaa agtccttata tccccgacca agctccaaaa tctagggata aatggaaaga
     4741 gcaagtgctt gatttttggt cttggctagt ggaagcgatc aaatctccta caagtaagtt
     4801 tgaaacaagt atcacacaca gttacacagc ctttctcttg ctcattctgt tttctgcatc
     4861 ttcctttttc tttagtatct atcacatcaa acatgcttac tatggacata tagcaagcat
     4921 taacagtcgc ttccctgagc agctagctcc tttaactctt ttttctatca tctctatcct
     4981 agtagcgaca acactcttct tcttttcatt cctcttgggt agtttcgttg tgagacgatt
     5041 tatccaccag gaaaaggact ggacactaga caaggttctc caacaatata gtcaactctt
     5101 ggcaattcca atcttcctca ctgctattgc tagtttcttt gctttctttg atagcctacg
     5161 atttacagcc ctcttgtgtg tgattagcat tggaatcatt ctgcttgcca gtctccatat
     5221 cattacaaga ccaagtcaag caagtgaaac cgactccttc tatcaattat tcttgtctgt
     5281 ccttgtgaac ggagttatta tcctcctctt ctttgtagct gaagtggcac tgattggaga
     5341 ttatcttcgt atcttggcct ttctttaaac ttcatactct tcgaaaatca aattcaaacc
     5401 acgtcagctt cgccttaccg tactcaagta cagcctgcgg ctagcttcct agtttgctct
     5461 ttgattttca ttgagtagca atcctgtcat ccttggcagg attttttcta tgtcaaaaaa
     5521 gcaagtcgat tgacctgctt ctgctttact tttcttgtgc taatgtttta aaatcttgtc
     5581 ctaggatggg ttgtatttct atttgattgg cgtctagttg gtggtaaaaa gatgggggta
     5641 atacctctaa gaattttgca tagtccaagc gagctatgga ttcatagtct tctggtttag
     5701 agccgtctcc tgtgatttga accaagtaaa tctcccactg gacttcctta tccaccaatt
     5761 gctcaatata ggctgcagga acaaacggtt ctctcggtaa aactgtcttg actccttgag
     5821 ctaaatggta aacgccatgc acttttcctt ctccatcctg atagaaggaa actcttgcaa
     5881 agtaggcatc tgtctcttga accgcacgca cacgcgcttc aaactgagcc aagccatctg
     5941 cctctaaaaa gcttttcaag ttctcacgac taaagaaaat aggattaaaa attccttggc
     6001 aaatcgtaaa acgagctaca gggtctgcta gataggcttg aatacttgct tggaaataag
     6061 cttcaaaatc aaaaccatct agcccttcaa tatcagtccc tgtataagca agcaaagcat
     6121 ctaaaaatga cttgataatc tgccagtctg tctttgttag taggtcagga agatcgacac
     6181 ggtaagcctt ttgaccatca gcataactga ccttaaagag aaattgtgat tgccccacta
     6241 tcgcacactc aatatactcg agacgattaa gaggctgacg aagatagact acatcatagc
     6301 tatgtgactc caaaccgtct agcaaggcca agatcgactt ggcattcaaa atctcttgtt
     6361 ctcctaaaat gctctgttta tttggaataa aaaatgtttt tgccataact ggactccttt
     6421 gaaatcgttt acatacagta taccttattt tcctgaaaga tacaaaaaca aactttagat
     6481 ttttgagtac aaagcataga aaaacagccc ctgaggactg tttgatctta tacagtagct
     6541 gcctgatcca agctttcacc gatagcggct aggcgctcaa tcacttcagc ttgtgtcaat
     6601 tcattttttg aaacatagcg gttacgtggg tgaacacggc actcgtgtga gcatccacga
     6661 aggtacttgt cttcattttc ttctgatgtc aagatacgac ggttacagaa gggatttcca
     6721 cagttaacat aacgttcaca tggtgttcca tcaaaccagt ctttccctac gatggttggg
     6781 ttgacatggt tgacatcaac ggcaatacgc tcgtcaaaga cgtacatttt cccatcccaa
     6841 agctcacctt gaacttctgg gtctttaccg taagttgcga ttcctccgtg caattggcca
     6901 acatctttgt aaccttcacg aaccatccag cctgagaatt tctcacagcg aacaccacct
     6961 gtacagtaaa ccacgacacg cttgtccatg aatttttctt tgttgtcacg gacccattgt
     7021 ggtaactcac ggaagttgcg aatatctggg cgaatagccc cacggaaatg tcctaggtcg
     7081 tactcataat cgttacgtgt gtcaaggaca acggtatctt tatcaagaag cgcttctttg
     7141 aactcttttg gagacaagta agcacctgtt gtttcaagtg ggttgatgtc attgtcaaag
     7201 tcgttgtctt ccaaaccaag gtggacaatt tctttcttgt agcgaacaaa catcttcttg
     7261 aaggcttgtt cactttcttc gtcaatcttg aaccagagat cttccattcc tggaaggctg
     7321 tgaacgtagt ccatgtattt ttgagttgtt tcatagtcac ctgaaactgt tccgttaatt
     7381 ccctcgtcag cgactaggat acggccttta aggccgattg atttacagaa agccaagtgg
     7441 tctgcagcaa attgctctgc attttcaatt ggagtataaa ggtagtaaag taagacacga
     7501 atatcttttg ccataagatt tgttctcttt tctattctta aattttctaa attttttatt
     7561 tatccatact agtatacctg cttgagaaaa cgaatgcaat ttatttgact ggaaattgta
     7621 gaggattctc gaaatttcct gaatggttaa aataaggaca agagaaaaca tggatatcta
     7681 tatccttgtg ccaaaaaaac cactgccctc cccagaccaa cctgaggaaa gcagtgattc
     7741 ttattttagg agttaggaat gaatacacga aatcaattta gctgattatt ttttgttttt
     7801 caagaattca tcgtattgtt tttgcatttc gttcaatact ttttcgtagg caccttcaga
     7861 tttcaatttt tccatcaatt ctggaatagc tttatctggg tctacagtac cagtgttgat
     7921 agctgtatca aattgttgca ttgtgttagc aatagctgag atttcagatt tcacattgtc
     7981 agtattgaag ataaatccaa gcgctggaga ttctttagct tctgccaatt ctttcttaga
     8041 attttcgatt tgttggtctg taacgttttc gttgatgtaa aggatccagt tgttaccagt
     8101 gttccatcca cccatgtgag tgtttccttt gtagtcatca agaacgcgaa cacggttttc
     8161 tttaccttca attttttccc agttcttgcc ttctggaccg taaacaagac cgttcaagag
     8221 ttctggattc gtattcaaga ggttcaagat ttccattgat ttttctttgt tcttagagtt
     8281 gtttgagatg acaaagttag caacttgtgt tgtttggttt ttcttgatga agttagtaat
     8341 tggtttgatt tggatatctt tgttggcaac acgtgaaagc aagctgttac cgtagtcagc
     8401 tggtcctact gtttcttcac gaacgaacca agtatcttgt tgaaggtcaa aggaagtatc
     8461 gcttgttgcg acgtcttttg gaatgtagcc agcttcatag aatttgtgaa gagtcttcaa
     8521 gtgttctttg aaacgaggca cttcgtaacg gtttacaact ttagtagtat cgccttcaag
     8581 gtcgataacg aatggaagac cgtttgctac tgggtagtca aagttatcag atgggatgaa
     8641 aactttacca atagcaaatg gtactacgtc tggagctttt tctttaattt gtttcaagac
     8701 tggctcaaga gtttcgtaag aagtaacacc tgaaatatcg ataccatatt tagcaaggag
     8761 agttccgttg aaggcaaagt tttgagatga tgcaacgttg gctgcaactg gaacagcgta
     8821 aatcttacca tttacagtat tacccttgat gtaagctggg tcaagtgctt tgtaaaggtc
     8881 tttaccttct tttttgtaca attctgtcaa gtcagcgtaa gcaccttttt gagcatttac
     8941 aatatagtta tctgcaaagg caatatcata gttttcacca gatgatgtga taactgacat
     9001 tttcttacca tagtcacccc agccaaggta ttggatatcc aatttggcac caactttttc
     9061 ttcaatgatt ttgttggcat ttgctaacaa ttcatccaag ttgtctggtt tgtcaccgat
     9121 ttggtacatt ttgataacag gtttgtcacc tgaatcagca gcttttttgc tgttacctgt
     9181 caaatttcca caagcagcaa gacctgcagc caaagcgact acactagcag atgcaaaagc
     9241 atattttttc cagtttttca tgataaaaac tccttttttt atttttaaac ttataaacaa
     9301 tgtaatgatc ttatactcaa tgaaaatcaa agagcaaact agaaaactag ccgcaggctg
     9361 ctcaaagcac tgctttgagg ttgtagataa gactgacgaa gtcagttaca tatatctacg
     9421 gcaaggcgac gttgacgcgg tttgaatttg attttcgaag agtattaact tcacacaagg
     9481 gaagttggga actgagaaat gttctttttc aataagcact attctttcac accaccgata
     9541 gtcaaacctt ttacaaagta gcgttggaag aatggataca aaatcgcgat tggaagggtt
     9601 gcaaccacaa ccatggccat acgacctgtt tctttcggta gagcaactcc cagttgacca
     9661 gtcaagccga ccgctttggc aatgtagtcc atattttgtt ggatttgcat gagcaaatat
     9721 tgcaatggat acaagttgtc actcttgatg taaagaaggg cgttgaacca gtcattccag
     9781 aaaccaagag ctgtcaaaag cgtgatggtt gcgatacctg gtagtgacaa tggcaaacag
     9841 atttggaaga aaatccgggc ctcactggca ccatcgatac gagccgattc gagaatggct
     9901 tctggaatgg tcttcttgaa gaaggaacgc atcaagatga tattgaatgg tgagagaagc
     9961 attggaacaa tcaaggccca aacagtgtca ccaagctgaa gtacacgggt caccatgata
    10021 taacctggta ccaaaccagc gttgaacaac atactgagaa ggacgaagat ggtaaaaaat
    10081 ctgcgatact taaaggttgt ccgtgaaata gcgtaggcat aggttgttgt gataaagaca
    10141 tttgtcaatg tcccaactac ggttacaaag acagagatga agagggcttg taggatttta
    10201 tccttaaact gtgccaaaaa ctcaaaaccg tctaagccaa attgggatgg gaagaagcta
    10261 tagccgtatt ggaggaggct tttctcgtct gtcactgaaa taatgataac gaatacaaaa
    10321 ggtaggatac aagagagggc aatcaaaccc gaaatgatac tgaagaagat atctgctttc
    10381 ttactgaagg agtgaatgcc gacattatca attttttctt ttttaatttt cttttttgcc
    10441 atattctcct cctttctaga acaaagctga gtttggatcg actcgtcttg caagcaagtt
    10501 tgataggata accagaatca aaccaacaac ggattggtaa agaccggctg ctgcagccat
    10561 accgatatct gctgtctgag tcaaaccatt aaaaacatat acgtccaaaa cgttggttac
    10621 attgtaaagc tgaccagcat tgtgtgggat ttgatagaag agaccgaagt ctgcgcggaa
    10681 gatatttccg actgcaagga tggtcaatac agttacaagc ggagtcaact gaggaatggt
    10741 tacgttgcga atacgttgcc acttgctagc tccgtccact gtcgctgctt cgtagtaggt
    10801 tggatcaatt cccatgatcg tcgcatagta catgacactg ctatatccaa aacctttcca
    10861 aatacctagg aaaagtagga gatagggcca gatgcccagg tcagcgtaga aattgacttc
    10921 tttgagacca agactttcca atagatgatt gaacacccct ttatcaatat ttaggaaggc
    10981 atctgtaaag aaactgatga taacccaaga caagaagtaa gggaacaaca tagaagtttg
    11041 gaaaatcttc accattctct tagaacggag ctcgctgagg ataatggcaa tccctacaga
    11101 tacaactaaa cctagaaaga taaagccaag attgtagagg acagtatttc gtgtgataat
    11161 aaaggcgtct cttgaactaa ataagaatct gaaattatcg agtccgaccc atttactatt
    11221 tatgatacta tctatgaaac cattactggt catgtggtag tctttgaagg caaccacgtt
    11281 cccaaatact ggaatgtaaa agaatagaat caaccagagt gcccctggca aaaccatcaa
    11341 gagaaagatc cagttgtctc tcaatgtttt tgaaaacttt ttcataattt cctccctttt
    11401 tattttgata tccatctaaa aattcttttt tagacttttg ataacgatta cattattagt
    11461 atactcctat ttgcaggtta ggttaaactc ctaattatag aaaaaactcc acaaattatg
    11521 tagcagattt aaaactttat caccactatc aaacaaatgt cctaaatcaa ttgtttattt
    11581 tatctctatt agcccagtga tggcgtcact ctgttataag catccaacaa cggggtatac
    11641 tgaaaaatct ccagactagg gaactcagcg atagttccta atctggagat ttttaatatg
    11701 ttattaggcg tttgctttca acttagcaat aacctcttta agattatcaa tcaactctgc
    11761 ttcagtatgc tcagagcctt tttcatctgc caagaacaaa actgcttttt gaagttcttc
    11821 ttgagagttt tcaaggacat ccttatctgc tgtttctttg cccgctgttt gtcctccatc
    11881 cttagttgct gactggcttt ccttaatgga ctctagggaa gcaatggcat ctttgactgt
    11941 ttgcaagata tcacgtaaac cttgctctgt caaactatca tctgtaagag ctttcttagc
    12001 ctctgccaaa accagacgtg ctgaatctgt ggtaggattc gatacacctg tcaatgatct
    12061 caaaagattt tctaaggttt gagtctgctt actaatacta gactaaaatc aaaaagcatt
    12121 acataacagt gatatgaaat caactaaaga agaaatccaa accatcaaaa cacttttaaa
    12181 agactctcgt acagctaaat atcataaacg ccttcaaatc gttctatagt aaaatgaaac
    12241 aagaacagga caaatagatc aggacagtca aatcgatttc taacaatgtt ttagaagtag
    12301 atgtgtactg ttctagtttc aatccattat atttcgtctg atgggcaaat cttataaaga
    12361 gattatagag cttttatagt gatttgaaat aagatgtgaa caactctatc aggaaagtca
    12421 aattaattta tagaaatatt ttaacagcca aggtgtactg ttataaattc aatacactat
    12481 acttggtggt ttagctctcc tgttttctct tttagcttta accagccata aatggtatta
    12541 cgtgagattt gaaaaacgtg tgatgcttct gttatactac ctgttcgctc acaataagag
    12601 agaacttttt tacgaaaatc tattgaatat gccataaaag attataccac attgtgtact
    12661 atttttggtt cattttacta tatcactttg gactatatcg gtctagttat gtctaaacga
    12721 ttatagctgg atactaagag aaatcaaaaa agcactaagg gagaacgtcc caaagtgctt
    12781 aatatcaagg cctcaaagcc tatcttattt aataaaatat tacaacattt tgttgtagaa
    12841 ttcaacgaca agtgcttcgt tgatttctgg gttgatttcg tcgcgttctg gcaagcgagc
    12901 caatgaacct tccaactttt cagcgtcgaa tgatacgaat gctggacgtc caagagtagc
    12961 ttctactgct tcaaggattg ctggaacttt caatgatttt tcacgaactg agatcacttg
    13021 acctggagtt acgcggtatg atgggatatc aacgcgtttc ccgtcaacaa ggatgtgacc
    13081 gtggtttacg aattgacgag cttgacgacg agtagtcgcg agaccaagac ggtaaacaac
    13141 gttatccaaa cgacgttcca aaagaagcat aaagttgaaa cctaggattc cgcctttgat
    13201 ttttgtagct tgtacgaaca agttacggaa ttgtttttca cctacaccgt aagtgaaacg
    13261 aagtttttgt ttttcagcca attgcaaacc gtattctgac aatttagaac ggttgtttgg
    13321 tccgtgttgt cctggtacgt agttacgacg tgccaattct ttacctgtac ctgtaagtga
    13381 aaggccaagg cgacgagctt gtttccaaga tggtcctgta taacgtgaca tatgaatgtc
    13441 ctcctgatat aaataatatt tcggtggaaa tagtcactta gaaagccctg attcgtgcag
    13501 atgcccttcg cctaaacagc caaggttact tgtcataaga cacctgttga cgagcttcat
    13561 gctttcctgc tgatatttca cacaaaatct attctaccat gaatagctgg atttgtaaag
    13621 gggttttagg ctttattttc actaccagag aggttgagaa cgagggtaaa ggtacttcct
    13681 agaccgtact ggctgctgac tgtgatttcc ccacccaatt gatgggccaa ttcacgcgca
    13741 atcgcaagtc ctaatccatg accacctgtc ttcatgttac gcgaagtttc gacacgataa
    13801 agacgtttga aaatattttc caaatcctct ggggcaatac cctgcccttc atcggtcaca
    13861 ctgattgaaa gctggtcctt ctccagctta gccaccactt ccagcttggt tcctggagca
    13921 gaatatttaa aagcgttatc gaccagattc accaagatac gagaaagctt agcataatct
    13981 ccctcaatcc gggcagactc tgggattacc tgcaagtgga catctcttct ctcctgctca
    14041 atcaaaaact gaaattcact catgcactca attaagagct tgtccagaaa aatactgtct
    14101 ttactggtag tttccacctg atttctagct gtgtttaggg tcaaaaaatt caactcctca
    14161 accagtttat tgagcctctc cgtctggcgt ccaatggttg ctagataatg agcttgctcc
    14221 gactccttga taatcccatc caaaatccct tctaccgtcg cttgaatcga agtgataggg
    14281 gtcttaatat catgcgacaa ctgggcaatc atcaagccct tttctcgttc gctttcttcc
    14341 aaggaatcaa agcttacctg caaatcatgg gacatctcat taaaagtttg ccctaattgc
    14401 tgaaattcta caggaccttg aacctccaaa tttgaaggaa aatccttggc cgctacccgc
    14461 ttggcatgct ccttgagttt gcccaacgac gtaaagactg gcaataggag aaagagacta
    14521 atcccagcac cgacaaggct ggcaacgatg gtcatcccaa gcaaaaagta aatctcgcct
    14581 ttcgcaatca gcattttttg aacagcccaa aaaacaacca aaatggttaa gagggttgaa
    14641 ataatatatc caaccaaaat ataacttttt agtttcatgt ttgtcctctc ggtttctcta
    14701 tcttatatcc caacccccaa actgtcttaa tagtgggcgt ttggtcacta ctatattttg
    14761 ccagctcctg tcgaagagca tggatatgca cattcaaggt attggtgtca tccacgtagt
    14821 cttctttcca gatcttttca tagaggtctg tcttggagaa aactcgctct ggattgctag
    14881 ctaaaatcca cagcaattca aatgatttaa cagttaaatc cagcatttct tctcctattt
    14941 gaacttcatg actactatga ttcattttta gattgccaag ggaaatcagc tctgtttcgc
    15001 ccccacgatg aaggcggcgc aaaatattgt ggacacgcaa aactagctca cgtgggctaa
    15061 aaggcttagc aataaaatca tctgctccca agctcaggcc gtaaatcttg tcctgttcac
    15121 tggtcttagc agtaataaat aggaaaggct gctctggtga taagtattga acctcactga
    15181 ttaaatcata accatccatc cgaggcatca tgacatctgt gataatcaaa tcaatcggtt
    15241 ttttcttgaa cagctctagc gcttccagtc catcatgggc taccaagacc tgataacctg
    15301 cctgaattaa gtatctctga tgaatatctg tgatttctac ctcgtcgtca acgagtaaaa
    15361 ttgtctttcc catctgtctc tcctttgata aaaacagtgc tatactgctt taagtataac
    15421 actattttta ttgttttacg atgattttaa tctgctattg aaagaaagtc ctgattgctg
    15481 attgagttgt taaactaatc ttagtcagcc cactatccat catttatttc cttagctgat
    15541 tagttttctt cttttttgtt tttcaaacct agaccaccga gtaaacctgc aagcgcaagc
    15601 ccaaggaaac caatacttgc cattgatgtt tgagtctcac cagtatttgg tagcatagct
    15661 ttatcctctg acatcatcgt atcagacatc ttgttagcag aagcagccat gttttcacct
    15721 gccatcgtgt tggtagaact tgtcatggtg tcagcaggca tgctatctgt agtacctgta
    15781 gcatgattgt gattcatcgg agtcacgcca gaaccagagt tagaaggtga taatgaacca
    15841 tttgctgtgt ctgatgtttc tttaacattt atcttaatag tgactttttt agttgctacg
    15901 atgttgtcca agtctggttt accgtctttg ttaccataga cattgactgt agcgctgtaa
    15961 gtttgagtac catttgctcg gaactggtca atgagcgctt gtttttcttt gccagctaca
    16021 tttccgtcca aggctacttg atagaagtat tgacctttgg tcttcacgtt ttcacctagt
    16081 ggagataggg ctgggttttt agcgtcgccg ttatctgacc atggtgcctt gtcagatgcc
    16141 ttgagcaaga gacgagtcaa cataccatca cctgcgaaga gttcgtatgg aatcacatgg
    16201 ttgacacctg ctgtgaatgg accttcaccc ttggcttttt ctaggtaggc tgctggaaca
    16261 tcgatactgt ctttaacgtt gtctgcaacg gctttttgaa ctgtttcttt agaaattaaa
    16321 ccgtttatgt taatagtgac ttttttagtt gctacgatgt tgtccaagtc tggtttaccg
    16381 tctttgttac catagacatt gactgtagcg ctgtaagttt gagtaccgtt tgctcggaac
    16441 tggtcaatga gcgcttgttt ttctttgcca gctacatttc cgtccaaggc tacttgatag
    16501 aagtattgac ctttggtctt cacgttttca cctagtggag atagagctgg gtttttagcg
    16561 tcaccgttat ctgaccacgg tgccttgtca gatgccttga gcaagagacg agtcaacata
    16621 ccatcacctg cgaagagttc gtatggaatc acatggttga cacctgctgt gaatggacct
    16681 tcacccttgg ctttttctaa gtaggctgct ggaacatcga tactgtc
//
LOCUS       DAYYQN010000044        16453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYYQN010000044 DAYYQN010000000
VERSION     DAYYQN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.044"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            73..870
                     /locus_tag="ACHBRQ_001476"
     CDS             73..870
                     /locus_tag="ACHBRQ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9593125.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1018..2277
                     /locus_tag="ACHBRQ_001477"
                     /pseudo
     CDS             1018..2277
                     /locus_tag="ACHBRQ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            2373..3647
                     /locus_tag="ACHBRQ_001478"
     CDS             2373..3647
                     /locus_tag="ACHBRQ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HFM9593126.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3657..4535
                     /gene="truB"
                     /locus_tag="ACHBRQ_001479"
     CDS             3657..4535
                     /gene="truB"
                     /locus_tag="ACHBRQ_001479"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9593127.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4796..5098
                     /locus_tag="ACHBRQ_001480"
     CDS             4796..5098
                     /locus_tag="ACHBRQ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9593128.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5206..5844
                     /gene="udk"
                     /locus_tag="ACHBRQ_001481"
     CDS             5206..5844
                     /gene="udk"
                     /locus_tag="ACHBRQ_001481"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9593129.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5972..7312
                     /gene="xseA"
                     /locus_tag="ACHBRQ_001482"
     CDS             5972..7312
                     /gene="xseA"
                     /locus_tag="ACHBRQ_001482"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9593130.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEKGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7290..7502
                     /locus_tag="ACHBRQ_001483"
     CDS             7290..7502
                     /locus_tag="ACHBRQ_001483"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9593131.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7499..8374
                     /locus_tag="ACHBRQ_001484"
     CDS             7499..8374
                     /locus_tag="ACHBRQ_001484"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9593132.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8367..9182
                     /locus_tag="ACHBRQ_001485"
     CDS             8367..9182
                     /locus_tag="ACHBRQ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9593133.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            9175..9606
                     /locus_tag="ACHBRQ_001486"
     CDS             9175..9606
                     /locus_tag="ACHBRQ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9593134.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9613..11280
                     /gene="recN"
                     /locus_tag="ACHBRQ_001487"
     CDS             9613..11280
                     /gene="recN"
                     /locus_tag="ACHBRQ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9593135.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11282..12010
                     /locus_tag="ACHBRQ_001488"
     CDS             11282..12010
                     /locus_tag="ACHBRQ_001488"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HFM9593136.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12089..13912
                     /gene="lepA"
                     /locus_tag="ACHBRQ_001489"
     CDS             12089..13912
                     /gene="lepA"
                     /locus_tag="ACHBRQ_001489"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9593137.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13993..14145)
                     /locus_tag="ACHBRQ_001490"
     CDS             complement(13993..14145)
                     /locus_tag="ACHBRQ_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593138.1"
                     /translation="MKIKEQTRKLAAGYSKHSFEVVDETDEVSNHTYGKATLTWFEEI
                     FEEYKN"
     gene            14325..14800
                     /locus_tag="ACHBRQ_001491"
                     /pseudo
     CDS             14325..14800
                     /locus_tag="ACHBRQ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14838..15140
                     /locus_tag="ACHBRQ_001492"
     CDS             14838..15140
                     /locus_tag="ACHBRQ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9593139.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            15148..15949
                     /locus_tag="ACHBRQ_001493"
                     /pseudo
     CDS             15148..15949
                     /locus_tag="ACHBRQ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            15965..16453
                     /locus_tag="ACHBRQ_001494"
                     /pseudo
     CDS             15965..16453
                     /locus_tag="ACHBRQ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
BASE COUNT         4961 a         2970 c         3835 g         4687 t
ORIGIN      
        1 aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag aaaaaagatt
       61 taggagttta ttatgatttc ttcagaattt atctcaaaga ttgaatttgc ttgcaataag
      121 aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat gttcgcaggt
      181 gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat taataaaatt
      241 gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt ggcctacatt
      301 gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc tggtagtttc
      361 ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac cttgttcaac
      421 cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta tgcgaatctg
      481 acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc aaatgaattg
      541 gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct gtcatttatt
      601 ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta catgtttgta
      661 ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt gaaattcagt
      721 gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca ctggggtgtg
      781 actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata tgccttcctc
      841 aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt cgtgcttttt
      901 tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa ctgatatttg
      961 taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg tcaccttatg
     1021 acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt ggctccagct
     1081 actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga tgacacgtag
     1141 gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg tatcaccaat
     1201 cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc aggtatgact
     1261 gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt ggctgcttcg
     1321 actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta tggtatcaca
     1381 acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa agacaatacc
     1441 aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga cctggaaaaa
     1501 ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac ttttgcaata
     1561 ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc tgcgactaag
     1621 tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg tcgttttgac
     1681 tggacggctt cagggaaatt ccctcaattt gttgacgagg gttcaagctg ccacaatttg
     1741 agctatattc gtgatgtggg tgcagcaacc tttattatag ctgttcgagt tcaattgctt
     1801 cgtgatacag gtgcagtctt gtcaccattc aatgctttcc tcttgctaca aagacttgaa
     1861 actctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt tgattttctt
     1921 gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag tccttatcat
     1981 gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt ccacgtcaaa
     2041 ggtgacgagg aagaagcacg caaggtcatt gataatttag aaatcttttc tgaccttgca
     2101 aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca cggtcaattg
     2161 tcagaaaaag acctagaagc agcaggtgtc acaccaaacc aaattcgtct gtcaatcggt
     2221 cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa aatttaaagt
     2281 aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca ggcatgatat
     2341 aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt cccaactgtg
     2401 gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa gtgagaacgg
     2461 cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg gctgagcaaa
     2521 aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt gcgcaattgc
     2581 agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa gttgaacaga
     2641 caagccatca ggccttattg gctaaggaca aggaagtaca ggctttggag aaccaattgg
     2701 ccactttgcg tttggagcat gaaaatcaac tgcaaaagac cctttctgac ctagaaaaag
     2761 aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag ttgtctttgg
     2821 cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa gtcgagtttt
     2881 ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta gaacagtatg
     2941 cagagagtga gtttaacaag gtccgtagtt tcgcctttcc aaatgcttac tttgagaagg
     3001 ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag tgtgatgaaa
     3061 atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac ggaacagaga
     3121 agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg gagaagaact
     3181 gtgagtatgc cgttttggtg accatgcttg aggccgataa tgactacttt aacacaggga
     3241 ttgttgacgt cagccacgag tatgaaaaaa tgtatgttgt tcgtcctcaa ttctttatcc
     3301 agttgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag caggagttgg
     3361 ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg gatgccttta
     3421 agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa gctattgatg
     3481 aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact acatctgaaa
     3541 accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa ttgacccgga
     3601 aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa gcaaaaatga
     3661 acggtattat taacttaaaa aaggaagcag gaatgacctc gcatgatgcg gtttttaaac
     3721 tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat ccggatgtgg
     3781 tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt atgcaggacg
     3841 agggtaagat ctatgagggg gaaatcactc tgggctattc cacgacgact gaggatgcta
     3901 gtggggaagt ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag cttgttgatg
     3961 aagcgattgc tagcttgact gggcctatta ctcagattcc ccctatgtat tcggcagtta
     4021 aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg gagcgtccag
     4081 aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat gatggccaac
     4141 ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac gtacatccgt actttgtcag
     4201 ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact cgtactagtg
     4261 ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa aaagtagagg
     4321 ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt gtcaaagttt
     4381 tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag ctagaccaaa
     4441 cggacaaaga attggctgcc tttgaagatg ataaattgtt agccattcta gaaaaacggg
     4501 gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa aaatcgggtg
     4561 atagataata attgcttgat aaaaccccat actaatagta gaatggtttt gggaattata
     4621 atattccaat tgttgcgagt tgtaggtact caaataatct attaggtact caaataatct
     4681 atatagaaat ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag
     4741 agtccattac aggttctggt gggaacttag gcccaggatt tggtgtgata attccatgat
     4801 gcgaaatgag tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa
     4861 tagtgagttg agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata
     4921 ccaaacggtc atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta
     4981 ccaggcacct aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga
     5041 aagagggaga ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa
     5101 ctgtcgaaca tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta
     5161 aaaaatgtga tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca
     5221 atcattattg gagtgacagg tggttctggt ggtggtaaaa ctagtgtttc aagagccatt
     5281 ttatcgcatt ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat
     5341 cagtctcatt tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt
     5401 gatacagact tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc
     5461 ccgacctacg actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa
     5521 gatgtcttta tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg
     5581 gatatcaaga tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt
     5641 gatatggagg agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc
     5701 aaaccaatgt accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct
     5761 gaaggggtta gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg
     5821 gaagaagctc gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt
     5881 ccctcataat ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat
     5941 gaaagagcaa gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt
     6001 gaccaagtat ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac
     6061 tggtcaagtt tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga
     6121 ccatgcagtt attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct
     6181 ggaagaagga atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag
     6241 ctactccatc atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt
     6301 tgaacaactt aagaaaaaat tgacagaaaa aggcctgttt caagaacgct tcaagcaagc
     6361 tctaccccaa ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg
     6421 agatattatc acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatcctac
     6481 caaggttcaa ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca
     6541 acgggacgat ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg
     6601 ggcctttaac gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc
     6661 tagtgtgggg catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc
     6721 aacgccaaca gcggcggctg aactggcaac acctgtgacc aagttggatg tattaactca
     6781 tttgcaaaat caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca
     6841 agaggctttg aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga
     6901 cggttatttg caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg
     6961 gatttctgat aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc
     7021 tgttaccaaa atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag
     7081 ccaaatggcg ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt
     7141 gctcatgttg gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga
     7201 gtccattgta gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg
     7261 agatggtcaa gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc
     7321 tagcagaact ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg
     7381 cgattactgc ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca
     7441 aggctgaaaa gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat
     7501 gaaaaagcaa gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca
     7561 gcagtttgcc tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg
     7621 tattcggcct tttctcttgt tagaagttct ggaagccttg caggttacca tcaaacctgc
     7681 tcacgcgcag gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga
     7741 ccttcctgct atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt
     7801 cggtgaagct atggccattt tggctggaga tgccttattc ttagacccat atgccttgat
     7861 tgcgcaggca gatttgccaa gtcagattaa ggtggacttg attgccaact tatcccttgc
     7921 ttcaggtagt ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca
     7981 cttgtctttg gaagaacttc agattattca tgccaataag actgggaagt tactagccta
     8041 tcccttccaa gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa
     8101 aactgtgggt gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac
     8161 agctagtttt gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac
     8221 ctatcctgcc ctgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca
     8281 agctaatgaa aaattagaag aaattgccca gcagcttccc tttgaaacag aatcgattgt
     8341 aagtgtagta gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata
     8401 aacaggggtt gtttgaaacg agagagcagg ccaagcgagg tgtaatggct ggcctagtcg
     8461 tagcagtcct taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg
     8521 aattaaaact caagggagag aaactcaagt atgtcagccg tggtggtttg aaactggaaa
     8581 aggccttgca ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct
     8641 ctactggagg ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg
     8701 atgttggtac caatcagttg gcttggaaat tacgccaaga cccacgagtt gttagcatgg
     8761 agcagttcaa tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca
     8821 gtattgatgt gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg
     8881 ctgatcaagg tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga
     8941 ttgggaaaaa tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa
     9001 cagctatggc agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag
     9061 gtggacatgg aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc
     9121 agatttttgc tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat
     9181 aaaaaagaga gacttgaaaa aattagaaga ttagtgacag attatcaaat cggcacgcaa
     9241 gaagaaattg tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc
     9301 cgagatatca aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac
     9361 gaattaccaa aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct
     9421 gaattgatgg ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa
     9481 gctcagttaa tcgatacttt tgcagacaag atttttagtt gtttgactga tgatagctcg
     9541 attttagtca ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat
     9601 tggtaggtct atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt
     9661 tccctcaatt ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg
     9721 attatcattg atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt
     9781 catggtgcac caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta
     9841 caggaaattt ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa
     9901 atcttgcaaa atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt
     9961 ttgcgagcta ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta
    10021 atgcgtcccc aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac
    10081 ttgaaagaaa cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg
    10141 gaagtcaaga aaaaccaaca ggaacacaag gaacgtatcg aaatgttgga atttcaaatg
    10201 gcagagattg aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga
    10261 gataaactcc tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg
    10321 gacaatgaag atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt
    10381 gtcgaagagt atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat
    10441 gttttagaag acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc
    10501 aatcgcctga tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat
    10561 ggtgggactg ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc
    10621 ttgacaggca ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc
    10681 aatcttgtca atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc
    10741 gaagctgaga ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt
    10801 cattttagta agggaaaatt cagtcgtggg ggaaatgaaa tggttgagtt ttatatttca
    10861 accaaccctg gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct
    10921 cgtctcatgt tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc
    10981 tttgatgagg tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt
    11041 cataaaattg gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg
    11101 attgcagatt atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact
    11161 gttcgtctct tgacagtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat
    11221 gatgtgacag aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa
    11281 gatgacagat tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct
    11341 tcttaaaacc tgggatggtc agacccagtt gctctttcta ggtgatttga ttgacagagg
    11401 tgaggatagt caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat
    11461 ctgtttgtct ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag
    11521 ttatgaccat tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc
    11581 cttggatgca ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcgtcaga
    11641 cttggtcgaa ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatatttgt
    11701 tcacgcaggt attgatttga ctttggacga ctggcatgaa acaacagatt ataaaaaagt
    11761 atggcttaga aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg
    11821 tcatacaccg gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac
    11881 agaagatggc aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat
    11941 tgtctttaca gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc
    12001 tgaagattag tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat
    12061 aaatagatac cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacaggg
    12121 gaagatccgt aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga
    12181 ccgcattttg gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga
    12241 tagcatggat ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa
    12301 ttacactgca aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt
    12361 tgactttacc tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt
    12421 cgatgcggct caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa
    12481 tgatttggaa atcatgccta tcattaataa aattgacctg ccggctgcag atccggagag
    12541 cgtgcgtaca gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc
    12601 tgccaaggct ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc
    12661 accaacgggt gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc
    12721 ttaccgtggg gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa
    12781 gattcagctc atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc
    12841 aaaagcggtt ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat
    12901 caagacggtt caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc
    12961 agaaccatta catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat
    13021 cgaatcaaac aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc
    13081 tagtcttcag tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt
    13141 tcttggactt ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga
    13201 tctcatcatg acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat
    13261 ggatgtgtct aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc
    13321 ttatgtcaag gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc
    13381 tcagcgtaag cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt
    13441 tatctatcaa attcctcttg ctgaaatcgt ctttgacttc tttgataaac ttaagtcttc
    13501 gacacgtggt tatgcaagtt ttgactacga attgtcagaa tatcgcccat ctaagctagt
    13561 gaaaatggat atccttctca acggagacaa ggtggatgcc ctcagcttta tcgttcataa
    13621 ggactttgct tatgaacgtg ggaaactcat cgttgataag ctcaagaaaa tcatccctcg
    13681 ccaacaattt gaggttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga
    13741 tatcaaggcc cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa
    13801 gcgtaaactc cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt
    13861 tgaagttccg caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag
    13921 aaatgccttt ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga
    13981 tggtctgctt ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    14041 cttaccgtat gtatggttac tgacttcgtc agtttcatct acaacctcaa aactgtgttt
    14101 tgagtaacct gcggctagct tcctagtttg ctctttgatt ttcattgagt attagaacag
    14161 aaaaatgctt ggagtatttg tttgtgtgtt tatttttata taacaaacta taaacacaac
    14221 aaaaatataa aaaaagagac aaaaaagaac agaaagtaat taacaacgat ttcatatagt
    14281 gttatactta tagcataaag ttaatggaaa gaagggaaaa ccttatggta ttagattatt
    14341 tctttgacaa aaaccttgtg ttttgcttag aagcggataa tcaagaataa ctctttgatc
    14401 aggtagcaag cttgttggag gaacgagaaa ttgtaactcc aacttatcgt gaagctttga
    14461 tcacgcgtga aaagtcattt ccaactggtt tagatatgga atttctagga aaggacttgc
    14521 aaatgtaatg attcctcaca cagatattgt tcataatcta gctgagaaag tggtggttgt
    14581 tcgattagag aaaccggtga ctttccataa tatgattgcg ccaggtaagg aagtagaagt
    14641 atccttgctc ttctttatca tcaataattc aagttcaagt caaacgaaca ttcttgctca
    14701 gttgatggac tttttcacag gaaatggaca tcttgaagac ctatcaaaaa tttccgaacc
    14761 agaaaaactt tatgcttata tagatgaagc aatcgcttaa tcttgtctat taaaaaataa
    14821 aatcggagga agtctatatg attaaaattc ttgctgcctg cggtgcaggt gttaactcaa
    14881 gtcaccaaat taaaagtgct ctagaagaag aactttcaaa ccgtggttac gatgttcact
    14941 gtgatgcagt tatggtgaaa gatgtaaacg aagaccttat gaaaggttac gatatcttta
    15001 caccaatcgc tgcaacagac cttggttttg aaccaggtat cccagttatc gaagctgggc
    15061 caatcttatt ccgtatccca gcaatgagcg ttccagtatt tgacaatatt agacttcctg
    15121 cgaaacaaaa tatggtatag tagttctatg aatgatgaag caagtaaaca actaactgat
    15181 gcacgattta agcgtcttgt tggtgttcag cgtaccactt ttgaagagat gttagctgta
    15241 ttaaaaacag cttatcaact taaacacgca aaaggtggac gaaaacctaa attaagccta
    15301 gaagaccttc ttatggccac tcttcaatag tgcgagaata tcgaacttat gaacaaattg
    15361 cggctgattt tggtattcac gaaagcaact ttatccgtcg gagccaatgg gttgaagtaa
    15421 ctcttgttca aagtggtgtt acggtttcaa gaactcctct cagctctgag gacacggtaa
    15481 tgattgatgc gacggaagta caaatcaatc gccctaaaaa agaattagcg aatgattctg
    15541 gtaaaaagaa atgccacgct atgaaggctc aagcgattgt cacaagtcaa gggagattgt
    15601 ttctttggat atcactgtga actattgtca tgatatgaag ttgttcaaaa tgagtcgcag
    15661 aaatatcgga caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat
    15721 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccgctaa cagttgaaga
    15781 taaaacctat aaccatgcgc tatctaagga gagaagcaag gttgagaaca tctttgccaa
    15841 agtaaaaacg tttaaaatga tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
    15901 acgaatgaat ttgattgctg gcattatcaa tcatgaacta ggattctagt tttgcaggaa
    15961 gtctaataaa gaaactagca aggtcaggcc ttactttatt ggtggacatc caggatttaa
    16021 ttgtcctcta cttgatgatg aagtctatga agattattat ctagagtttg agaaagaaga
    16081 gacttgctct gttccacgtc ctttcccaga aacaggtatg ttggatttcc aagatagaag
    16141 tccatggcta gagggacaaa aagaaataga tcttagttat gatcttttca gcaaagatgc
    16201 agtgactttg gatgaattgc aatctcgaac aattgccctt cgttctctta aacatgatag
    16261 gggattgaaa gtgcattttg cagaatttcc aaacctcatc atctggtcaa ctttgaacaa
    16321 gggacctttc attacctttg aaccatggtc tggcttgtca acattccttg aagaaggaga
    16381 tcatttagaa gataagaaga atgtttgtct cttagaagcc aatcaggttg aagaattagg
    16441 gtttgagata gaa
//
LOCUS       DAYYQN010000045        15889 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYYQN010000045 DAYYQN010000000
VERSION     DAYYQN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.045"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            106..3903
                     /gene="spuA"
                     /locus_tag="ACHBRQ_001495"
     CDS             106..3903
                     /gene="spuA"
                     /locus_tag="ACHBRQ_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HFM9593140.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(4003..5052)
                     /locus_tag="ACHBRQ_001496"
     CDS             complement(4003..5052)
                     /locus_tag="ACHBRQ_001496"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM9593141.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5171..6979)
                     /gene="glmS"
                     /locus_tag="ACHBRQ_001497"
     CDS             complement(5171..6979)
                     /gene="glmS"
                     /locus_tag="ACHBRQ_001497"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9593142.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLYAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7171..8550)
                     /gene="bglA"
                     /locus_tag="ACHBRQ_001498"
     CDS             complement(7171..8550)
                     /gene="bglA"
                     /locus_tag="ACHBRQ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9593143.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8650..10503)
                     /locus_tag="ACHBRQ_001499"
     CDS             complement(8650..10503)
                     /locus_tag="ACHBRQ_001499"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9593144.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10516..11775)
                     /gene="rseP"
                     /locus_tag="ACHBRQ_001500"
     CDS             complement(10516..11775)
                     /gene="rseP"
                     /locus_tag="ACHBRQ_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9593145.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11797..12600)
                     /locus_tag="ACHBRQ_001501"
     CDS             complement(11797..12600)
                     /locus_tag="ACHBRQ_001501"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9593146.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12609..13367)
                     /locus_tag="ACHBRQ_001502"
     CDS             complement(12609..13367)
                     /locus_tag="ACHBRQ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9593147.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALIKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13598..14170)
                     /locus_tag="ACHBRQ_001503"
     CDS             complement(13598..14170)
                     /locus_tag="ACHBRQ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HFM9593148.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14151..15149)
                     /gene="ruvB"
                     /locus_tag="ACHBRQ_001504"
     CDS             complement(14151..15149)
                     /gene="ruvB"
                     /locus_tag="ACHBRQ_001504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9593149.1"
                     /translation="MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15283..15375
                     /locus_tag="ACHBRQ_001505"
     CDS             15283..15375
                     /locus_tag="ACHBRQ_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9593150.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         4830 a         3748 c         3003 g         4308 t
ORIGIN      
        1 aacttctcaa aagtaagaat ttttttcaca aatcaaagaa aacgcttgca tattgtttta
       61 ctacatgcta tactgatata gaaatttatt gattggagaa tcattatgag aaaaacacca
      121 tctcacactg agaaaaaaat ggtctacagc atccgttccc tcaaaaatgg aactggttct
      181 gtccttatcg gcgcaagcct tgtcttgctt gccatggcta caccaactat ctcatccgac
      241 gaaagtacac caaccactaa cgaacccaac aacagaaata caaccaccct tgcccaacct
      301 cttactgata cagcagctgg ctctggtaag aacgaaagtg atatttcttc acctagaaat
      361 gcaaacgctt ccctagagaa aacagaagaa aaacctgcaa cagagccaac tacttctact
      421 agtccagtaa caactgaaac taaggcagaa gagcccatcg aagataacta cttccgtatc
      481 catgtcaaaa aacttcctga agaaaacaag gatgctcaag gactatggac ttgggacgat
      541 gttgaaaaac catctgaaaa ctggccaaac ggagctttgt ccttcaagga tgccaagaaa
      601 gatgactacg gctattacct agatgtcaaa ttaaagggag aacaagccaa gaaaattagc
      661 ttcctcatca acaatacagc tggaaaaaat ctaaccggcg ataaatctgt agaaaaacta
      721 gttccaaaaa tgaacgaggc ttggttagac caagattaca aggttttctc ttacgagcca
      781 cagcctgcag gaactgttcg cgtcaactac taccgcacag atggcaacta tgacaagaaa
      841 tctctctggt actggggaga tgtaaaaaat ccaagtagcg ctcaatggcc tgacggaaca
      901 gactttacgg ctacaggcaa atatggccgc tatatcgaca ttcctcttaa tgaagccgca
      961 agagaatttg gatttttatt actagatgag agcaaacaag gagacgacgt gaaaatccgt
     1021 aaagaaaatt ataagttcac agatttgaaa aatcatagcc aaattttcct aaaagacgat
     1081 gatgaatcga tttacacaaa tccatactat gtccatgata tccgtatgac aggagcccaa
     1141 cacgtaggca cttctagcat tgaaagtagc ttttcaacac ttgtcggtgc taaaaaagaa
     1201 gatatcctca aacactccaa catcactaat cacctaggaa acaaggtaac tattaccgat
     1261 gttgcaatcg atgaagctgg taagaaagtg acctacagcg gagatttctc tgacacaaaa
     1321 catccttata ctgttagcta caattccgac caattcacta ccaaaacaag ctggcgcctg
     1381 aaagatgaga catacagcta tgatggcaaa ctgggagctg acctaaaaga agaaggaaaa
     1441 caagttgatt tgaccctttg gtcaccaagt gctgataagg tttctgttgt tgtctacgac
     1501 aagaatgacc ctgacaaagt agttggaact gtcgctctcg aaaaagggga aagaggaact
     1561 tggaaacaaa ctctagacag cacaaacaaa ctcggaatca cagatttcac tggctactat
     1621 tatcaatacc aaatcgagcg tcaaggtaaa actgttcttg cactcgatcc ttacgctaaa
     1681 tctcttgctg cttggaatag cgacgatgcc aaaattgacg atgcccataa agtggctaaa
     1741 gccgcctttg tagatccagc taaactcgga cctcaagact tgacttatgg taagattcac
     1801 aatttcaaga ctcgtgaaga cgccgttatc tacgaagctc atgtgcgtga cttcacttca
     1861 gatcctgcca ttgcaaaaga cttgaccaaa ccatttggga cttttgaagc cttcattgaa
     1921 aaactagact atctcaaaga cttgggtgta acccatatcc agctccttcc agtcttgtct
     1981 tactactttg tcaatgaatt gaaaaaccat gaacgcttgt ctgactacgc ttcaagcaac
     2041 agcaactaca actggggata tgaccctcaa aactacttct ccttgactgg tatgtactca
     2101 agcgatccta agaatccaga aaaacgaatc gcagaattta aaaacctcat caacgaaatc
     2161 cacaaacgtg gtatgggagc tatcctagat gtcgtttata accacacagc caaagtcgat
     2221 atctttgaag acctagagcc aaactactac cactttatgg atgccgatgg cacacctcga
     2281 actagctttg gtggtggacg cttggggaca acccaccata tgaccaaacg gctcctagtt
     2341 gactctatca aatacctagt tgatacctac aaagtggatg gcttccgctt cgatatgatg
     2401 ggagaccatg acgccgcttc tatcgaagaa gcttacaagg ctgcacgcgc cctcaatcca
     2461 aacctcatca tgcttggtga aggttggaga acctatgccg gtgatgaaaa catgcctact
     2521 aaggctgctg accaagattg gatgaaacat accgatactg tcgctgtctt ttcagatgac
     2581 atccgtaaca acctcaaatc tggttatcca aacgaaggtc aacctgcctt tatcacaggt
     2641 ggcaagcgtg atgtcaacac catctttaaa aatctcattg ctcaaccaac taactttgaa
     2701 gctgacagcc ctggagatgt catccaatac atcgcagccc atgataactt gaccctcttt
     2761 gacatcattg cccagtctat caaaaaagac ccaagcaagg ctgagaacta tgctgaaatc
     2821 caccgtcgtt tacgacttgg aaatctcatg gtcttgacag ctcaaggaac tccatttatc
     2881 cactccggtc aggaatatgg acgtactaaa caattccgtg acccagccta caagactcca
     2941 gtagcagagg ataaggttcc aaacaaatct cacttgttgc gtgataagga cggcaaccca
     3001 tttgactatc cttacttcat ccatgattct tacgattcta gcgacgcagt caacaagttt
     3061 gactggacta aggctacaga tggtaaagct tatcctgaaa atgtcaagag ccgtgactat
     3121 atgaaaggtt tgattgccct tcgtcaatct acagatgcct tccgacttaa gagtcttcaa
     3181 gatatcaaag accgtgtcca cctcatcact gtcccaggcc aaaatggtgt ggaaaaagag
     3241 gatgtagtga ttggctacca aatcactgct ccaaacggtg atatctacgc agtctttgtc
     3301 aatgcggatg aaaaagctcg cgaatttaat ttgggaactg cctttgcaca tctaagaaat
     3361 gcggaagttt tggcagatga aaaccaagca ggatcagtcg gaattgccaa cccgaaagga
     3421 cttgaatgga ctgaaaaagg cttgaaattg aatgccctta cagctactgt tcttcgagtc
     3481 tctcaaaatg gaactagcca tgagtcaact gcagaagaga aaccagactc aaccccttcc
     3541 aagcctgaac atcaaaatga agcttctcac cctgcacatc aagacccagc tccagaagct
     3601 agacctgatt ctactaaacc agatgccaaa gtagctgatg cggaaaataa acctagccaa
     3661 gctacagctg attcacaagc tgaacaacca gcacaagaag cacaagaagc atctgtaaaa
     3721 gaagcggttc gaaacgaatc ggtagaaaac tctagcaagg aaaatatacc tgcaacccca
     3781 gataaacaag ctgaacttcc aaatacagga atcaaaaacg aaaacaaact cctatttgca
     3841 ggaatcagcc tccttgcgct ccttggtctc ggtttcttac taaaaaataa aaaagagaac
     3901 taaactagcc ctcctataga aaaatccccc aagcattata gctcggggga ttaatttttg
     3961 tacaatattt gttgtcctaa taaacttgat taggattttt tattaagcct ctttcatagc
     4021 aaaataagct cgtactttgg gtgcaacttg tgttccgaag agttcaatag ctctcagaac
     4081 ctggtcatga ggcatagaac caagcggtag atgaagcatg aagcggtcca atcctaaatc
     4141 ctctatcatg cgaatcaatt tttcggccac ctgatctgga ttgccaacaa acatggcgcc
     4201 atttggccct acctgctcca aatattgctc ataacgcaat tcctgccagt gcggacggtc
     4261 tttggaaata gcatccacca cttgcttagt cggatggaaa taatctttca ccgcctgctc
     4321 accatcttcc gcaatccacc cccaagaatg ggctcccact ttcaagtctt tgtcagcatg
     4381 gcccgcttcg cttccaatct cacgataagc ctgaatcaac tttttaaaat aacgtggatt
     4441 accaccaata atagcatata caatcggtag accagcctga gcaatcttca ctgttgattc
     4501 gacatgacca cctgtagcta tccacaaggg caatttgtcc tgaactggac gaggataaac
     4561 ttctttacca gcaatcgttt gagtcaatcg accttgccag tctaacttgg tcttttcatt
     4621 gactaactga agcaagtcta atttctcatc aaaaagagag tcgtagtctt tcaagtcata
     4681 accaaacaga gggaaagatt ccgtgaaaga gccccttcca gccataatct ccgatcgtcc
     4741 atttgacaaa gcatcgatag tggcatactg ttggaacaaa cgaatcggat ccatgcttga
     4801 cagaatgctg actgcactgg tcaaacggat tttcttggta ttgactgccc cagcggccag
     4861 gacaatctct ggggctgata ctgcaaaatc cgcccgatgg tgctcgccaa tcccatatac
     4921 gtccaaacca accttgtcag ccagctcaat ctctgccacc aactggcgaa tgcgttcagc
     4981 atgactgtaa gtttgtccag tcccttcaag ctccgttatt tccccaaatg ttgaaattcc
     5041 caattctacc attgtgattc tccttgtcta tctctgtcct tcaatttgaa aaattattct
     5101 aacacgaatc ttgagtacaa gcaaccaatt tgctcattag aaaaagccta gataactaga
     5161 cttttttagc ttattctacc gttactgatt tagcaaggtt acgtggttta tccacatcga
     5221 ggccacggtg gagggttgca aagtaagcaa ccaattgcgt cggtacgacc attgaaattg
     5281 gtgagaggta agggtgtaca gtcgtaagga cgatatcgtc ggtatcttta gcaacatttt
     5341 cttctgcgat agtgaggacc ttagcaccac gggctgccac ctcttggata tttccacgag
     5401 tgtggttggc aaggactgga tctgacaaga gagccaagac aggtgttcct tcttcaatca
     5461 aggcaatggt tccgtgcttg agttctcctg ccgcaaaacc ttcacactgg atataagaaa
     5521 tctctttgag tttgagactt gcttccatgg ctacgtagta atcttgacca cgtccgatgt
     5581 aaaaggcgtt acgagttgtt tcaagaagtt cacgaacctt ggcttcaatg gtttctttct
     5641 ctgaaagagt tgattcgata gactgagcta cgattgacaa ttcatgaacc aggtcaaagg
     5701 cttgcgcttt agcattacca tttgcttctc cgactgcttt tgcaaggaag gcaagggctg
     5761 cgatttgcgc tgtataggct ttagttgatg ccacggcaat ttcaggacct gcgtaaagga
     5821 gcatggtata gttggcttca cgtgagaggg ttgaacctgg aacgtttgtc actgttaagc
     5881 ttggaattcc catttcatta gccttgacca aaacttgacg actatccgct gtttcaccag
     5941 attggctgat aaagatgaag agtggtttct tgctgagaag tggcataccg tagccccact
     6001 cagatgagat gccaagttca actggtgtat ctgtcaattc ttccaacatt ttcttagaag
     6061 caaatcctgc atggtaagat gttccagctg caaggatgta gatgcggtct gcgtcttgaa
     6121 cagccttaat gatagcagga tcaatcacta cttgaccagc atcatccgtg taggcttgaa
     6181 tgagtttacg cataacagtt ggttgctcat caatttcctt aagcatgtag taaggataag
     6241 ttcccttacc gatatctgac aagtcaagtt ccgcagtata gctagcacgt tcacgactgt
     6301 taccatcata gtcttgaact tccacgctat cagccttgac gattaccaac tcttggtcat
     6361 gaatttccat gtattggttg gtttcacgaa tcatagccat agcatctgag cagaccatat
     6421 tgtagccttc cccaagacca atcaaaagtg gagatttgtt tttcgctaca tagatgacat
     6481 ctggattttc agagtcaatc aaggcaaagg catatgaacc acggataata tgaagagctt
     6541 ttttaaaggc ttcaagaact gagagaccgt cttcttccgc aaattttcca atcaaatgta
     6601 cggcgatttc cgtatctgtt tgccctttga agtggtgccc agcaaggtat tcttctttaa
     6661 tttcaaggta gttttcaatc accccattgt gaaccaagac aaaacgttct gtctcagagc
     6721 ggtgtgggtg agcattgtct tccgttggtt tcccatgagt tgcccaacga gtatgtccga
     6781 taccagttgt tccctcaaca ccagctgtct tggcagacaa ttctgcgata cgaccaacag
     6841 ccttcaccaa atggttatca gcaccatcta ggacaaaaat tcccgcagaa tcatagccac
     6901 ggtattcaag cttttcaagc ccttgaatca aaatatcagt tgcatttgtg tttccaacaa
     6961 caccaacaat tccacacata gtatatacga cacaggcaag ctgtgctttc tccttaaaat
     7021 tggtatagtc taattcatct tttatagaat cagcaaaaac agtatatact tgtttctttc
     7081 acttgtcaag agtaaaaatt ggtatagttc aaattaagct cctgtaagca aaaaactctg
     7141 accgattggg ataatcagtc agagtccttt ttaaaatcca ttattatcgc ttaattcttt
     7201 gaaccagtgg cctgatttct tcagacgacg ttcttgcgtt tccaagtcta attcgaccaa
     7261 accatagcga tttttatagc tgttgagcca tgaccagcag tcaataaagg tccaaatcaa
     7321 gtagccctta cagttggcac catcttcaat ggcacggtga agttcacgaa gatgaccttt
     7381 tacaaagtca atacggtaat catcttgaat cattccatct tgacggaatt tttcttcccc
     7441 ttcaacaccc ataccattct cagtcaacat ccactcaata ttgccataat tttccttgat
     7501 attttgggcg atgtcataaa tcccttgctc ataaatctcc caaccacggt gagaattgat
     7561 tttacgtcca ggcatcacat aaggctcgta aaaatgttct ggtaagagtg gactatctgg
     7621 atgcttagca aatcgaggag ccataacacg caaaggttga tagtagttca caccaaggaa
     7681 gtccaccgta ttatcacgaa tgagttccaa ctcttcctct gtagcatcag gtaaaagacc
     7741 gtgttcatgc aagatttcta ccaactcctg tggataagtc cccaagacag atggatctaa
     7801 gaaagattgg gcctgaaaaa gggccgcaat acgagctgcc ttgacatcag caggatgctg
     7861 gctacgtgga taagccggtg tcaagttgag gacaatccca atcttggaat caggcaaaag
     7921 ttcatggcaa gccttaacag cccggctgct ggccaattgt gtatgatagg ctaccttaac
     7981 agctgcctct gcatccacct tatgtggata atgggcatca taaaaataac caaattctac
     8041 aggaacgatg ggctcgttaa aggtaatcca ttgatccact aaatctccat aagtctcaaa
     8101 acaaaaacga gcatagtctt cataggctga gactgtcgcc ttattttccc aaccatcacc
     8161 atcctcttga agggcaaaag gtaaatcaaa atgatagaga ttgactaaca gacgaattcc
     8221 tttagcctta atagcctcaa agaccttacg ataaaaatcc acaccttgag ggttgacttt
     8281 tccacagcct tgtggaaaaa tccgtgacca ctgaatagaa gtccgaaaga ctgtgtgacc
     8341 agtctctaac aaaagctcaa tatcccgctc ccaattttca taaaaagtcg atgtcttatc
     8401 tgaaccaatc ccattatagt aacgatttgg ctccacttgg aaccagtaat cccagagatt
     8461 gtctccctta ccgtcaccag ctacacgtcc ttctgtctgc ggtccagaag tagaggatcc
     8521 ccagacaaaa tcctttggaa atcttagcat aacatttacc tctttatcta ctcatttctc
     8581 ccattataca gaaaaaacaa ggtaaaaact agttacattt tttccttgtt tttcttctga
     8641 ttatagtttt tatttcttgc ttaggatttc aagcgtttca agcacgttat ctgcatgaac
     8701 ctcaatggtg tcaccagttg ccttgatctt aacttctaca atgccatcgg ccgctttttt
     8761 accaacagtg atacggattg gaagaccaat caagtcacta tcgctaaatt taacaccgac
     8821 acgttcgtta cggtcatctg tcaagacttc ataaccagct cccatcaagc ttgcttcaag
     8881 tttttctgtc aaggcttgcg cttcttcatc cttgacattg acagtaatca aatgcacatc
     8941 aaatggtgcc aattctttag ggaaattgat tccccaagcg taacggtatt caccttttgg
     9001 cgttttgtta acaaagaggc gagcgtgttg ctccatcact gctgaaagaa gacggctgac
     9061 accgataccg taacatccca tgatgattgg cacagcacga ccattttcat ccaagacatc
     9121 tgctcccatg cttgctgagt agcgagttcc gagtttgaaa atatgaccga tctcaatacc
     9181 acgcgcaaag ttaaggacac cttgtccatc tggggaaatt tcaccctcac gaacttcacg
     9241 gatatccaca tattctgcag taaaatcacg gcctgggttc acaccagtca agtggtagcc
     9301 atcttcgtta gcaccgacaa ctgcattgcg aacatcttgc accttacgat ctgcaataat
     9361 tttaatattc tctggcaaac caactggtcc aagtgaacca aatcctgctt gaacaacatt
     9421 cgccacttct tcttcgctag caacgtcaaa gaaatctgct cccaagtgat ttttcaactt
     9481 gacttcgttg agttggtcat ttccaactag aagggctgca acaagctcac catctgcaat
     9541 gtagaagagg gttttaatcg tttgttcttc tggaacattg aggaaggctg caacttcatc
     9601 aattgattta acatctggcg ttgcaacacg agtaacttct tcttcagcga caacacggtt
     9661 gcttggtttg tactcgtttg ttgccatttc taagttagct gcatagctag actcacttga
     9721 gtaagcaatg gtatcttcac cagagactat ccatttgagc aattctgcct tgatttcttc
     9781 ttgcacttct gcaggaattt cgtcaaatga ggcaactgac ttgtccaaga caacccagcg
     9841 gtcaaggtct gtacgagcag atgtaatggc cataaattct tggctatcct taccacccat
     9901 ggctccaccg tcaccaataa tagccttgaa gtctaaacca ctacgagtga aaatacgctc
     9961 ataggctgct ttgtactcat cataaacact atccaaacta tcatagttag cgtggaaact
    10021 ataagcatcc ttcatgataa actcacgtgt acgaagaagt ccattacgtg ggcgtttttc
    10081 atcacgatac ttgggctgaa tttgataaag gttgagtggc aattgcttgt aagatttaac
    10141 agaatcacgg acaatagctg taaaggtttc ttcgtgagtt ggacctaaga taaagtctga
    10201 tttttcacgg ttttttagtt tgtaaaggtc ttcaccatag gtttcgtaac gacctgattc
    10261 acgccacaat tctgcactaa gaagggttgg agccaacatc tcaacagcac caatcttttc
    10321 gaattcttgg cgcatgatgt ttttagcttt ttcaatcaca cggttggcaa gtggtagata
    10381 agaataaaca cctgctgaaa cttggcgaac ataaccagca cgcaacataa gagcatggct
    10441 gataacttga gcatcgcttg gcatttcgcg aagcgttggg ataggcattt tactttgttt
    10501 cataatattc ctcgattatc taaaaaagag tcgcataatg tcattccaag tcacagcaat
    10561 catcaagaca accatgatga ccactccggc caaggtgaca taggtttcaa tttcttgttt
    10621 caatggtttg tggcggatgg cttctaggat attgagcaca atcttaccac catccaaggc
    10681 tggaatcgga ataagattaa aaatcccaat attgatggaa atcattgcca agaagtacaa
    10741 gatattctca attccatttt tagcagcatc actacttgcc ttaaagatag caacaggtcc
    10801 acccaacttg ttcaaatccg gttggaaaat cagatttttc agagctgaga gaattcggag
    10861 agctgagtca gcagcagttg taaaaccacc tacaaacatg gatagaaaat ctgacttaac
    10921 ccccggttga acacctagaa ggtaacgacc ttgactatct tcgggtgtaa cagtgacttg
    10981 tttgtcactc cccttttcag aaatagtcac atccaaagtc ggtgccgtct tatctttggt
    11041 ttctgtttcc acagcttgga tcaagctttc ccagttgcta acctcatgtg agccaatctt
    11101 ggtaatttgt gccgtttctg gtactcctac cttggccaag gcaccttggg gcatgatatg
    11161 gaactgattg gtatcaacat ctctgacacc accctgcata aagattaaaa cccaaaaaac
    11221 aacgacacct aagataaaat tgttcatagg acctgcaaaa ttggtaatca gtttgcccca
    11281 gatagtcgca ttttgatatt gaacatctaa aggtgcaatc cgaacctcag taccatctgc
    11341 ttccacaacc gttgcatcgt gatccactgc aaatgttttt tcttcttcca gaaccaatcc
    11401 tttgataaag agcttgtctt caaaatcaaa ctgggtcacc tgcataggga gggctgtttg
    11461 atccaatttt ttacctgaga gattgatgcg tttaacctta ccatcatcag caagtgtcaa
    11521 actaacaggc gttcctgtct tgatttcagt tgtatcatca ccccaaccgg ccatgcggac
    11581 atagccaccc agaggcaaga ttcgaatggt ataggccgtt ccatccttgc caatgtgagc
    11641 aaaaatttta ggtcccatac cgatggcaaa ttcacgtact aaaatccctg atttcttggc
    11701 aaagtagaag tgcccgaact cgtgcaccac tacaataatc ccgaaaacca gaataaaggt
    11761 taaaattccg agcatagcgt ttcctccgtc ttttgattaa aagagtccaa ataagtgcat
    11821 gattggaaat acaagcaaca tactatcgaa acgatccaaa acaccaccat gtccagggat
    11881 aaatttccca gaatccttaa caccaaaatg acgtttgatc gaactttcta gtaaatcacc
    11941 aaattgtcca gcaatgctaa agaaaatagc aaagactgac atcttgtaaa ttccatatgg
    12001 aagagcaact gtactgtcaa ctatcataaa gataatggtt actaaaattg ctcctaaaat
    12061 accacccaag gcaccctcaa gggttttatt aggcgatacc cttggtgcta actttcgttt
    12121 cccatagttc atcccaacaa gataggcacc actgtctgtc gcccagacga tacacaaggc
    12181 taagagagcc ttgtccaaac ctgcaacacg agcatctagt aaagcattaa atccaaagcc
    12241 cacgtagaag ctcatagcaa gagggaaaac cgcatcctca atcgtataag acttgctaaa
    12301 aacggtcgtt cctaacatga ttgaaatcaa aacactatag gcaaccacat tcccatcaac
    12361 tggcaaaaaa gtcaggtaat tctccaaggg aatggttaat gcaaaggttg caaagagagt
    12421 caagaggccc tccatcgtca tggtcgctag acctcttatc ttcaaaagtt catgcatggc
    12481 tagcatggct atgattccga ttgctatctg aagcaagagg cccccaatca ttaaaattgg
    12541 taggaaaata gccagggcaa tccctgcaaa caaggttctt ttctgtaaat cctgggtcat
    12601 atttcctcct aaactcctcc aaatcggcga tggcgacgat tataggcaag aatagcttcc
    12661 tgcaaggctg cttcgtcaaa atcaggccat aaggtgtccg taaaataaag ctcactatag
    12721 gctccctgcc atggaaggaa attgctcaaa cgcaattctc cactagtacg gataatcaag
    12781 tctgggtctc gtaagtcctt aggcaaatgc tgggtaaaga gatagttgcc aatcaattcc
    12841 tctgtgatgt cacctgggtt gattttggca tctaaaacat cctgggaaat caacttaagc
    12901 gcctgtgtaa tctcagcacg tccaccatag ttaagagcaa aattaagaat caatcctgtg
    12961 ttgttcttag tcaattcctc agccttgatt aaagcttcaa aggtttgctt aggcaggcgg
    13021 tctgtctccc caatcatttg aatcttaaca ttattcgcat gtagttccgg gacataatta
    13081 tcataaaact ctactggcaa gttcatgata aacttgactt cctgatctgg acgggtccag
    13141 ttttccgtag aaaaagcata gaccgtaata accttgacgc ccagtttgtt ggctgccttg
    13201 gtcacggttt gcaatgcttc catgcccgcc ttatgtccaa aaactcgcgg ttgcatacgt
    13261 tttttagccc aacggccatt gccatccatg atgatgccga tatgagcagg aacctgtgtc
    13321 ggaacctcta cttccacagc cttatctttc ttaaaaaatc caaacatgat cttattccta
    13381 ttcaaaaatc tatcgtttca ttataccata tttccccatt ttcttctatc actaagctat
    13441 ttattctcag gcaccaagcc catttttcaa aaaaataagc cgcctgattg ggcgacttta
    13501 tttttatagg gagattatta tgaaaaagtt ttaggagttt aagttaaggt cttcttaact
    13561 tatgaactta gtgtacactc cctagcttaa agtttcctta agtattttta aaaatcaaat
    13621 ttttccattt ctcctgccaa tttttcttgg ataaacgtgt ttgatagagt tccattcggt
    13681 cttcattttc taagaaatga ggagttggac gaacttgaaa attcaaaata tcctccaaac
    13741 cataaggtgc atagagttca aaatctgatt cttcattcaa gcgcagtcca actgtcgtac
    13801 accgttctgg atacttactc atagcatcac gagaactggt ataggaagca gtgtgaggac
    13861 tgtgctgatg catatagacc tgatttttca attcccactg gtactgagga aaatcctctc
    13921 gcagcttttt ctccagtaat aaggtttcct cataagaaaa atctggatca aagaaaatca
    13981 catctatatc tgtttcatga tcaaaagggg atttgtctga caaaagattc caaatgaaat
    14041 ttctgacaga acctgctgcc aaccacgagt ctttcagacc aaggtctcgg atgatcgtca
    14101 gaatggccat catatctgga ttttctctaa aagcctctaa gatttcttgc ttatttttca
    14161 ctgtattcat aacctaagtg ctcatatgcc ttagcagtcg ccacccgtcc agaccgtgtc
    14221 cgcatgataa aacctttttg aatcaagtaa ggctcataca tatcttcaac tgtctcacgc
    14281 tcttcggcga tattcacaga aagagttcct agaccaacag gtcctccact gtacatctca
    14341 atcatggtac gaaggatttt ttgatccaca tagtccaaac cttcatggtc aacatccagc
    14401 atagtcaaag ccttatcggt aataatatca tcaattaccc cattccccat tatctgggca
    14461 aaatcgcgca cgcgcttgag gagacgattg gcaatacgag gggtcccacg actacgtagg
    14521 gccaactcag atgctgcctc atgagtgatt tccatctcaa aaatatctgc cgtccgctcg
    14581 acaatttctg tcaagccagc atgggcataa tactccatat ggcctgtaat cccaaaacgt
    14641 gcccgtagcg gattggagag cataccagcc cgagtcgtcg caccaatcaa ggtaaaaggt
    14701 ggtaactcca aatgaacact acgactaccc tcaccagccc caatcataat atcgatgtag
    14761 aagtcctcca tagcactata aagcacctct tccactgaca ttggcaaacg atggatctca
    14821 tcaataaaaa gtacatcccc aggctctaac tcattcaaaa tagctaccag atctccggct
    14881 ttttcaatga ctggacccga cgtctgctta agattgactc ccagttcgtt ggcaataaca
    14941 aaggccatgg tcgttttccc gagacctgga ggcccaaata agagcacatg atccagcgct
    15001 tcatcccgca ttttggcagc ttcaataaag atttgtagct ggtccttgac cttatcctgt
    15061 ccgatatatt cacgtaaata ctgaggacgg agcgtgcgtt ctactaactc ctcatccccc
    15121 attatctcat tatctaaaat tctactcatg gctctattat atcaaaaaac caagccacaa
    15181 acaaaaaagc cacctgattg ggtgactcct aagtttagca cttatgtggt ataatattct
    15241 acgacactgc tacaccgcct acgaaaggag gtgagatagc ccatgatgga attagtactc
    15301 aaaactatca tcggaccaat tgtggtcggt gtcgttcttc gtatagtcga taaatggcta
    15361 aacaaggaca aatagtgtca aaaaagacct caagcttatt tggtcgtgag cttggggtct
    15421 tttctagcct atgatataga actagtactc aattcctttt tattatccca tagttcacga
    15481 attttgtcaa aggtttacat tttcttcaac cgctgtacga caagacggtt aagattaaga
    15541 gaacgttaag gattctatca atttcataga aattttgatt tcgtaaacga agagacaatc
    15601 ttacatgtta cttctcattt aatacgccac tactagacaa gcaaaatcat tattacagta
    15661 gttccagttc ttcaattaac agtcacttac aatcaaattg agtttgaact agctgaagcg
    15721 accacagacc tatttcttag ttatattcgc taaaaaagtc cccgccaatt ccccgaccaa
    15781 aatccgaaaa ataccgaaaa atatcgaaaa attattttta gaatagtccc caaaagcctg
    15841 aaatagagct aaaaaactcc acctgattgg gtgaagttaa gggagatta
//
LOCUS       DAYYQN010000046        15344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYYQN010000046 DAYYQN010000000
VERSION     DAYYQN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.046"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            358..705
                     /gene="ssrA"
                     /locus_tag="ACHBRQ_001506"
     tmRNA           358..705
                     /gene="ssrA"
                     /locus_tag="ACHBRQ_001506"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(888..975)
                     /locus_tag="ACHBRQ_001507"
     tRNA            complement(888..975)
                     /locus_tag="ACHBRQ_001507"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Ser,seq:gga)
     gene            1137..1877
                     /locus_tag="ACHBRQ_001508"
     CDS             1137..1877
                     /locus_tag="ACHBRQ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9593151.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1874..2785
                     /gene="pfkB"
                     /locus_tag="ACHBRQ_001509"
     CDS             1874..2785
                     /gene="pfkB"
                     /locus_tag="ACHBRQ_001509"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9593152.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2782..4734
                     /locus_tag="ACHBRQ_001510"
     CDS             2782..4734
                     /locus_tag="ACHBRQ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9593153.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4871..7123
                     /locus_tag="ACHBRQ_001511"
     CDS             4871..7123
                     /locus_tag="ACHBRQ_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9593154.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAK
                     EALEYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(7168..7602)
                     /locus_tag="ACHBRQ_001512"
     CDS             complement(7168..7602)
                     /locus_tag="ACHBRQ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HFM9593155.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            7740..8882
                     /locus_tag="ACHBRQ_001513"
     CDS             7740..8882
                     /locus_tag="ACHBRQ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9593156.1"
                     /translation="MIYFDNSATTKPYPEALETYVQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPADT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8891..10105
                     /gene="thiI"
                     /locus_tag="ACHBRQ_001514"
     CDS             8891..10105
                     /gene="thiI"
                     /locus_tag="ACHBRQ_001514"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9593157.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10240..11064
                     /locus_tag="ACHBRQ_001515"
     CDS             10240..11064
                     /locus_tag="ACHBRQ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9593158.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGYSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            11086..11825
                     /locus_tag="ACHBRQ_001516"
                     /pseudo
     CDS             11086..11825
                     /locus_tag="ACHBRQ_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11838..13004
                     /locus_tag="ACHBRQ_001517"
     CDS             11838..13004
                     /locus_tag="ACHBRQ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFM9593159.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYLFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYIAIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            13264..13389
                     /locus_tag="ACHBRQ_001518"
     CDS             13264..13389
                     /locus_tag="ACHBRQ_001518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593160.1"
                     /translation="MKIKEQTRKLAAGFSKHCFEVVDRTDRVSIIYLRQGEADVV"
     gene            13512..15005
                     /locus_tag="ACHBRQ_001519"
     CDS             13512..15005
                     /locus_tag="ACHBRQ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFM9593161.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKKSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            15018..>15344
                     /locus_tag="ACHBRQ_001520"
     CDS             15018..>15344
                     /locus_tag="ACHBRQ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9593162.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKS"
BASE COUNT         4581 a         2832 c         3418 g         4513 t
ORIGIN      
        1 ttcttatttc attttactat attttggtgc aataagtgaa aagtagtccg aataatataa
       61 ggattgattt tatagttttt aaactcaaat gaattaaaat aaagagagta cgaaaattct
      121 catctgaaag tattttagaa taattctctt cgtgaatttc ttcaaaacag atagcttcat
      181 cttaggtatg tgttaattgg atttcgtaag ctctatttac aatcgtaaag ttatgcactg
      241 agaaagttgt agttagtctt ccagtttgct aaatttctcg aatagtatct tttttaggta
      301 tgtgatttct ttttgcattt ttgagttaga taaggtataa tggttttatt gtcttttggg
      361 gtcgttacgg attcgacagg cattatgagg catattttgc gactcgtgtg gcgacgtaaa
      421 cgctcagtta aatataactg caacaaataa cacttcttac gctctagctg cctaaaaacc
      481 agcaggcgtg acccgatttg gattgctcgt gttcaatgac aggtcttatt attagcgaga
      541 tacgattaag ccttgtctag cggtttgata agagattgat agactcgcag tttctagact
      601 tgagttatgt gtcgaggggc tgttaaaata atacataacc tatggttgta gacaaatatg
      661 ttggcaggtg tttggacgtg ggttcgactc ccaccggctc cattattcct ttgcattctt
      721 ttgcattcct tggtaaaacg ttgttaaatc aacgtttttt atttttatct ttggtattca
      781 tttgcattct tttgctaaaa agggagtcac aaacagaccc tattttaaaa aaaggataga
      841 aaaaaggata caacatttgt cgcatcctaa aaataatctt ttttcgacgg aagacatggg
      901 attcgaaccc acgcacgcta ttacacgcct accgcgtttc caacacggcc tcttaagcct
      961 cttgagtaat cttccaatac ttactcaaat agtctaccat aaaggctctt atcttgcaat
     1021 aaaaattcta gaaataagaa aaatgataga ttttgaaaga aaatgataaa aaatgcttga
     1081 cttcaaaaga aagtatgata gaatgaatag tgtaaacgat aacaggaggt gattcggtgt
     1141 taaaaacaga gcggaagcaa ctgattttag aggagttaaa tcaacatcat gtagtttctc
     1201 tagaaaaatt agttagtttg ctagaaacgt cagaatcaac ggttcgaaga gacttggatg
     1261 agttggaagc ggaaaacaag cttcgtcgtg tgcatggtgg agcagaactc ccccattcct
     1321 tacaggaaga agaaaccatt caagaaaaat ctgtcaaaaa ccttcaagaa aagaagttgc
     1381 tggctcagaa agcagcctct ctcattaaag aaaaagatgt catctttatc gatgctggaa
     1441 caacaactgc ttttttgatt catgaattgg tcaataagaa tattacagtt gtgaccaact
     1501 ccattcacca tgccgctcag ttggttgaaa agcagattcc aactgtcatg gttggaggaa
     1561 acgtcaagac ggcgacagat gctagtatcg gtggcgttgc tcttaaccag attaaccaat
     1621 tgcactttga ccgtgccttt atcggaatga atggtgttga cgatggctat tatacgactc
     1681 ctgatatgga ggaaggagct gtgaaaagag ctattttgga gaatgccaag cagacctacg
     1741 tcttggtgga ttcgtcaaaa attggacaaa cttgctttgc caaggtagca ccactcaaac
     1801 gcgctatcgt tatcactagt caagggcatg agctcttgca ggttattaag gagaaaacgg
     1861 aggtaataga agtatgattt atacagtcac actcaatcca tccattgact atatcgttcg
     1921 tttggaccaa gtcaaagttg gtagtgtaaa tcgtatggac agtgatgata agtttgctgg
     1981 tgggaaagga atcaatgtca gccgtgtctt gaaacgtttg aatataccaa atacagcgac
     2041 gggatttatc ggtggcttta ctggtaaatt tatcacagat actttagcag aggaagaaat
     2101 cgagacacgt tttgtccagg tggcagaaga tactcgtatc aatgttaaaa tcaaagcaga
     2161 ccaagaaaca gaaatcaacg gaacgggtcc aactgttgaa ccggttcagc tagaagaatt
     2221 gaaagctatt ttatctagtc tgacagcaga agatacagtt gtctttgcag gttcaagtgc
     2281 taaaaatcta ggcaatgtta tctataagga tttgatttcc ttgacgcgcc agactggtgc
     2341 gcaagtggtc tgtgactttg aaggacagac cttaattgat agtttggact accagcctct
     2401 tcttgtaaaa ccaaacaatc atgaacttgg agcgattttt ggggttaaac tcgaaagttt
     2461 agatgaaatt gagaaatacg ctcgtgagtt actggctaag ggtgctcaaa atgttattat
     2521 ctctatggct ggtgatggcg cccttcttgt cacatctgag ggagcttact tcgctaaacc
     2581 aatcaaagga acagtcaaaa attcagttgg agctggtgat tctatggttg ctggattcac
     2641 aggtgaattt gtcaaatcaa aagacgcagt agaagccttc aaatggggag tggcttgcgg
     2701 aacggcaact accttctcag atgacttggc aacggcggaa tttattaaag aaacatatgg
     2761 aaaagttgag gtagaaaaac gatgaaaatt caagatttat tgagaaaaga tgtcatgttg
     2821 ctggatttgc aggcaactga aaaaacagct gtcatcgacg agatgattaa aaatttgaca
     2881 gagcacggtt atgtaacaga ttttgaaaca tttaaagaag gaattttggc gcgtgaagct
     2941 ttgacttcta ctggtttggg tgatggaatc gcaatgcctc acagcaaaaa cgctgctgtc
     3001 aaagaagcga cagttctatt tgctaagtca aataagggtg ttgactacga gagcttggat
     3061 ggacaagcaa ctgacctctt cttcatgatt gcagctccag aaggtgccaa tgatactcac
     3121 ttggcagcct tggcagaatt gtctcaatac ttgatgaaag acggttttgc agacaaactt
     3181 cgtcaagcaa catctgcaga ccaagttatc gaactttttg accaagcttc agaaaaaact
     3241 gaggaacttg ttcaagcacc tgctaatgac tctggtgact ttatcgtagc tgttacagct
     3301 tgtacaacag gtattgccca cacttacatg gcccaagaag cccttcaaaa agtagctgct
     3361 gaaatggggg ttggtatcaa ggtcgaaacc aacggtgcta gcggtgttgg aaatcaacta
     3421 actgcagaag atatccgtaa ggctaaagct attatcattg cagcagacaa ggccgttgaa
     3481 atggatcgat ttgatggaaa accattgatc aatcgtccag ttgctgacgg tatccgtaag
     3541 acagaagagc taattaactt ggctctttca ggagatactg aagtctaccg tgccgctaat
     3601 ggtgcaaaag ctgcaacagc ctctaacgaa aaacaaagcc ttggtggtgc cttctacaaa
     3661 cacttgatga gtggtgtatc tcaaatgtta ccattcgtta tcggtggtgg tatcatgatt
     3721 gcccttgcct tcttgattga cggtgctttg agtgttccaa atgaaaacct tggcaatctt
     3781 ggttcttacc atgagttagc ttctatgttc atgaaaattg gtggagctgc ctttggtttg
     3841 atgcttccag tctttgcggg ttatgttgcc tactctattg ctgaaaaacc aggtttggta
     3901 gcaggtttcg tggctggtgc tattgccaaa gaaggttttg cctttggtaa aattccttat
     3961 gccgcaggtg gtgaagcaac ttcaactctt gcaggtgtct catctggttt cctaggtgcc
     4021 cttgttggtg gatttatcgc aggtgccttg gttcttgcca tcaagaaata cgttaaagtt
     4081 cctcgttcac tcgaaggtgc taaatcaatc cttctattgc cacttcttgg aacaatcttg
     4141 acaggatttg ttatgctagc tgtgaatatc ccaatggctg caatcaacac tgctatgaat
     4201 gacttcctag gcggtcttgg aggaggttca gctgtccttc ttggtatcgt ccttggtgga
     4261 atgatggctg ttgacatggg tggaccagtt aataaagcag cttatgtctt tggtacaggt
     4321 acgcttgcag caactgtttc ttcaggtggt tctgtagcca tggcagcagt tatggctgga
     4381 ggaatggtgc caccacttgc aatctttgtc gcaactcttc ttttcaaaga taaatttact
     4441 aaggaagaac gtaactctgg tttgacaaac atcatcatgg gcttgtcatt tatcactgag
     4501 ggagcgattc catttggtgc cgctgaccca gctcgtgcga ttccaagctt catccttggt
     4561 tcagcagtag caggtggact cgttggtctt actggtatca aactcatggc gccacacgga
     4621 ggaatcttcg ttatcgccct tacttcaaat gctctccttt acctcgtttc tgtcttggta
     4681 ggagcaatcg taagtggtgt ggtttatggt tacctacgca aaccacaagc ataaaaaata
     4741 gaaaaatgaa aagattggac cgtttggtgc agtctttttc tcttcccgaa atgcctgtga
     4801 aatatggtat aatagaagaa tggcaaacaa gaatacaagt acaacaagac ggagaccgtc
     4861 taaagcagaa ctggaaagaa aagaagcgat tcaacgaatg ttgatttcgt taggaattgc
     4921 gattttattg attttcgcag ccttcaaatt aggggctgca ggtataaccc tttataattt
     4981 aattcgcttg ctagtgggta gcctagctta tctggcgata ttcggcctat taatctatct
     5041 cttctttttc aagtggatac gaaaacagga aggactctta tctggctttt tcaccatatt
     5101 tgctggctta ctcttgattt ttgaggccta cttggtttgg aaatatggtt tggacaagtc
     5161 cgttctaaaa gggaccatgg ctcaggttgt gacagatctg actggttttc gaacgactag
     5221 ttttgctgga gggggcttga tcggggtcgc tctttatatt ccaacagcct ttctcttttc
     5281 aaatatcgga acttacttta ttggttctat cttgatttta gtgggttctc tcctagtcag
     5341 cccttggtct gtttacgata ttgctgaatt tttcagtaga ggctttgcca aatggtggga
     5401 agggcacgag cgtcgaaaag aggaacgctt tgtcaaacaa gaagaaaaag ctcgccaaaa
     5461 ggctgagaaa gaggctagat tagaacaaga agagactgaa aaagccttac tcgatttgcc
     5521 tcctgttgat atggaaacgg gtgaaattct gacagaggaa gctgttcaaa atcttccacc
     5581 tattccagaa gaaaagtggg tggaaccaga aatcatcctg cctcaagctg aacttaaatt
     5641 ccctgaacag gaagatgact cagatgacga agatgttcag gtcgattttt cagccaaaga
     5701 agcccttgaa tacaaacttc caagcttaca actctttgca ccagataaac caaaagatca
     5761 gtctaaagag aagaaaattg tcagagaaaa tatcaaaatc ttagaagcaa cctttgctag
     5821 ctttggtatt aaggtaacag ttgaacgggc cgaaattggg ccatcagtga ccaagtatga
     5881 agtcaagccg gctgttggtg taagggtcaa ccgcatttcc aatctatcag atgacctcgc
     5941 tctagccttg gctgctaagg atgtccggat tgaagcacca atccctggga aatccctaat
     6001 cggaattgaa gtgcccaact ccgatattgc cactgtatct ttccgagaac tatgggaaca
     6061 atcgcaaacg aaagcagaaa atttcttgga aattccttta gggaaggctg ttaatggaac
     6121 cgcaagagct tttgaccttt ctaaaatgcc ccacttgcta gttgcaggtt caacgggttc
     6181 agggaagtca gtagcagtta acggcattat tgctagcatt ctcatgaagg cgagaccaga
     6241 tcaagttaaa tttatgatgg tcgatcccaa gatggttgag ttatctgttt acaatgatat
     6301 tccccacctc ttgattccag tcgtgaccaa tccacgcaaa gccagcaagg ctctgcaaaa
     6361 ggttgtggat gaaatggaaa accgttatga actctttgcc aaggtgggag ttcggaatat
     6421 tgcaggtttt aatgccaagg tagaagagtt caattcccag tctgagtaca agcaaattcc
     6481 gctaccattc attgtcgtga ttgtggatga gttggctgac ctcatgatgg tggccagcaa
     6541 ggaagtggaa gatgctatca tccgtcttgg gcagaaggcg cgtgctgcag gtatccacat
     6601 gattcttgca actcagcgtc catctgttga tgtcatctct ggtttgatta aggccaatgt
     6661 tccatctcgt gtagcatttg cggtttcatc aggaacagac tcccgtacga ttttggatga
     6721 aaatggagca gaaaaacttc ttggtcgagg agacatgctc tttaaaccga ttgatgaaaa
     6781 tcatccagtt cgtctccaag gctcctttat ctcggatgac gatgttgagc gcattgtgaa
     6841 cttcatcaag gctcaggcag atgcagacta cgatgagagt tttgatccag gtgaggtttc
     6901 tgaaaatgaa ggagaatttt cggatggaga tgctggtggt gatccgcttt ttgaagaagc
     6961 taagtctttg gttatcgaaa cacagaaagc cagtgcgtct atgattcagc gtcgtttatc
     7021 agttggattt aaccgtgcga cccgtctcat ggaagaactg gagatagcag gtgtcatcgg
     7081 tccagctgaa ggtaccaaac caagaaaagt tttacaacaa taaaaaaata gcttctttcc
     7141 aagtttggaa ggaggctatt ttagtgatta ttgattagtt ttattttccg aagttggggt
     7201 attggactgt ttttcgtttt cagtagcagg tttacttgga gtaggagcag gagtagaaga
     7261 atcctgagtt gatgttttct gctcttcttt tttctcttcc ttgacgctgg attttggcgt
     7321 ttcttcttgt tgtgtttttt cttgagaagt cttattttcc ttattgggac tagtattttc
     7381 ctgaggggat tccttttgaa tttctttgac aattgttgtc gtctggcttg ttgtaggttc
     7441 ctttttagta tttttgttat tatccaaggc gttcataatc gtgatactag cggtaatcaa
     7501 gccaaggata ctagcgatag tcgcaacggt tttttgaaag actgtaaagc cttttttgat
     7561 gtgttctgtt tttggttttg aagttctacg ataattagac atacattctc tcctttgatt
     7621 atgacttatt atactctgtt tctttaaaaa aatcttaaaa aaagagaaat tgccctttct
     7681 tgtcgcaagc tccgtttcat gatacaatag agggagtgat tttagaaagc gtgagaaaca
     7741 tgatttactt tgataattca gcgacgacca agccttatcc tgaagccctt gaaacctatg
     7801 tgcaggtcgc ttcaaaaatt ttaggaaacc catctagtct ccatcgtttg ggagaccaag
     7861 caacacgaat cttagatgct tctcgccaac agattgcaga tttaatcggt aagaaaagcg
     7921 atgaaatctt ctttacctct ggtggaacag aaggggataa ctgggttatc aagggtgtgg
     7981 cctttgaaaa agctcagttt ggcaagcaca tcattgtttc agccattgaa catccagcag
     8041 tcaaagagtc agccctctgg ttgaaaagtc aaggatttga agtggatttt gctccagttg
     8101 ataagaaagg cttggtcgat gttgaggcgt tagcaggttt gatacggcct gatacaatcc
     8161 tcgtttccat catggctgtg aacaatgaaa tcggctctat ccaacctatt gaggctattt
     8221 cagaattctt ggcagacaag ccgactattt ccttccacgt tgatgcggtt caggcgcttg
     8281 ccaaaattcc gactgaaaag tatctgacag aacgggtgga ttgcgcgacc ttctcgagtc
     8341 ataaatttca tggtgtccgt ggtgttggct ttgtctatat caaatctggc aagaagatta
     8401 cacctcttct tacaggtggt ggccaggagc gagattatcg ttcgacaact gaaaatgtgg
     8461 cagggattgc agcgacagcc aaggccctcc gtttgtctat ggaaaagcta gatatcttta
     8521 ggagcaagac tgggcagatg aaggcagtga ttcgccaagc tcttctgaac tatccggata
     8581 tttttgtctt ttcagatgag gaaaactttg cacctcatat tctgactttt ggaatcaaag
     8641 gtgttcgagg tgaagtcatc gttcacgcct ttgaagacta tgatattttc atctcaacaa
     8701 cctcagcttg ttcatctaag gcaggaaaac cagccgatac cttgattgcc atgggagtgg
     8761 acaaagataa ggccaagtca gctgtgcgtc ttagcctaga cttggaaaat gatatgagtc
     8821 aggtcgagca gtttttgacc aagttaaaat tgatttacaa tcaaactaga aaagtaagat
     8881 aggagcattc atgcagtatt cagaaattat gattcgctac ggagagttgt caaccaaggg
     8941 taaaaaccgt atgcgtttca tcaataaact tcgtaataat atttcggacg ttttgtctat
     9001 ctatacccaa gttaaggtaa cagcagatcg cgaccgtgcc cacgcttacc tcaatggagc
     9061 tgattacaca gcagttgcag aatctctcaa acaagttttt ggaattcaaa acttttctcc
     9121 tgtttataag gttgaaaaat ctgtagaagt tttgaagtct tctgtccaag agattatgcg
     9181 ggacatctac aaggaaggta tgacctttaa gatttctagc aagcgtagcg accacaactt
     9241 tgaacttgat agtcgtgaac tcaaccaaac acttggaggg gctgtattcg aagccattcc
     9301 aaatgtgcaa gttcaaatga aaagtcctga catcaatctt caggtggaga ttcgtgaaga
     9361 agcagcctat ctttcttatg aaaccattcg tggggctggt ggtttgccag ttggaacttc
     9421 aggtaaaggg atgctcatgt tgtcaggagg gattgactca cctgtagcag gttatcttgc
     9481 tcttaagcgt ggggtggata tcgaggcagt tcactttgct agtccaccat atactagtcc
     9541 tggtgccctc aagaaagcgc aggacttgac ccgtaaattg accaagtttg gcggaaatat
     9601 ccagtttatc gaggtgcctt tcacagagat tcaagaggaa atcaaagcca aagcgccaga
     9661 agcttatttg atgactctaa ctcgtcgctt tatgatgcgg attactgacc gtattcgtga
     9721 ggtacgaaat ggtttggtta tcatcaatgg ggaaagtcta ggtcaagtag ccagccaaac
     9781 ccttgaaagt atgaaggcta tcaatgctgt taccaacact cccatcattc gtcctgtggt
     9841 taccatggac aagttggaaa tcattgacat cgcccaggaa atcgatacct ttgacatttc
     9901 aatccaaccg tttgaagact gttgtaccat ttttgcacca gatcgtccaa aaacaaatcc
     9961 taaaattaag aatgcggagc agtacgaagc gcgtatggat gttgaaggct tggttgagcg
    10021 agcagtggct ggaatcatga ttactgaaat cacacctcaa gccgaaaaag atgaagttga
    10081 tgacttgatt gacaatctgc tctaattcag aaaatccaaa agaatagcga aaatcagtaa
    10141 aaaaagttag ttttttctct aaaaacaggt aaaaaactaa ctttttttat ttttatgata
    10201 taatgatata aaattttgaa tatagagagt tttctgacaa tgaatcaatc ctacttttat
    10261 ctaaaaatga aagaacacaa actcaaggtt ccttatacag gtaaggagcg ccgtgtacgt
    10321 attcttcttc ctaaagatta tgagaaagat acagaccgtt cctatcctgt tgtatacttt
    10381 catgacgggc aaaatgtttt ttatagcaaa gagtctttca ttggatattc atggaagatt
    10441 atcccagcta tcaaacgaaa tccggatatc agtcgcatga ttgtcgttgc tattgacaat
    10501 gatggtatgg ggcgaatgaa tgagtatgcg gcttggaagt tccaagaatc tcctatccca
    10561 gagcagcagt ttggtggtaa gggtgtggag tatgctgagt ttgtcatgga ggtggtcaag
    10621 ccttttatcg atgagaccta tcgtacaaaa gcagactgcc agcatacggc tatgattggt
    10681 tcctcactag gaggcaatat tacccagttt atcggtttgg aataccaaga ccaaattggt
    10741 tgcttgggcg ttttttcatc tgcaaactgg ctccaccaag aagcctttaa ccgctatatc
    10801 gagtgccaga aactatcgcc tgaccagcgc atcttcatct atgtaggaac agaagaagca
    10861 gatgatacag acaagacctt gatggatggc aatatcaaac aagcctatat cgactcgtcg
    10921 ctttgctatt accatgattt gatagcaggg ggagtacatc tggataatct tgtgctaaaa
    10981 gttcagtctg gtgccattca tagtgaaatc ccttggtcag aaaatctacc agattgtctg
    11041 agattttttg cagaaaaatg gtaagttaag aaaggaaaaa acgaaatgca tattgaacat
    11101 cttagccact ggagtggtca tcctaaccgt gaaatgtacc ttaaccgtta tggacatggt
    11161 gggattccag ttgtggtctt tgcttcatca ggtggtagtc acaacgaata ctatgatttt
    11221 ggcatgattg atgcctgtgc ttcctttatc gaggaaggcc ttgtccagtt ctttacccta
    11281 tctagtgtgg atagtgagag ctggttggct acttggaaaa atgctcatga ccaagcggaa
    11341 atgcaccgtg cctacgaatg ttatgtgatt gaggaggcca ttcttttatc aagcacaaga
    11401 caggttggtt tgatggcatg atgacgacag gttgctctat gggagcctat catgcactca
    11461 atttcttcct ccagcatcca gatgtcttta ccaaagtgat tgctctcagt ggtgtttacg
    11521 acgcacgttt ctttgtcggt gattactaca acgatgatgc tatttaccaa aactcgccag
    11581 tagattatat ttggaaccaa aacgacggct ggtttattga ccgttaccgt caggcagaga
    11641 ttgtgctgtg tacggggctt ggagcctggg aacaagatgg tttgccatcc ttttacaagc
    11701 tcaaagaagc ctttgacaag aaacaaattc cagcctggtt tgctgaatgg ggacatgatg
    11761 tcgcccatga ctgggaatgg tggcgtaaac aaatgcctta tttcctcggt aatctctatt
    11821 tataaaagga gttacctatg aattaccttg ttatttctcc ctactatcca caaaactttc
    11881 aacagtttac catcgaacta gctaataaag gcatcacagt cttgggaatt ggtcaagagt
    11941 cttacgagca attggatgag cccttgcgca atagcttgac cgagtatttt cgtgttgata
    12001 atcttgagaa catagatgaa gtcaaacgtg cagttgcttt tctcttttat aaacatggtc
    12061 caattgaccg catcgagtct cacaatgaat actggcttga gctagacgca acactcagag
    12121 aacaattcaa tgtttttggt gccaaaccag aggatctcaa aaagacgaaa tataagtctg
    12181 aaatgaagaa acttttcaaa aaagcaggtg ttcctgtggt acctggagct gttatcaaga
    12241 cggaagcaga tgttgatcaa gcagtgaaag aaatcggtct tccaatgatt gccaaacctg
    12301 ataatggagt gggagcagcc gcaaccttta aacttgagac agaagacgat atcaatcact
    12361 tcaagcaaga atgggaccat tcaacccttt atttatttga aaaatttgtc acttccagcg
    12421 aaatctgtac ctttgacggg ctcgtggaca aggatggaaa gattgtcttc tcaacaacct
    12481 ttgactacgc ctatacaccg cttgacctca tgatttataa gatggacaat tcttattatg
    12541 tgctcaagga tatggatcct aaactgcgca agtatgggga agcaattgtc aaagaatttg
    12601 gtatgaaaga acggtttttc catattgagt tcttccgtga gggggacgat tatattgcca
    12661 tcgagtacaa taaccgccct gcaggtggtt ttaccattga tgtttataac tttgctcatt
    12721 ccttggacct ttatcgtggc tatgcagcta ttgtagcagg agaggagttc ccggcgtcag
    12781 actttgaaac tcagtattgt ttggctactt ctcgccgtgc aaatgctcac tatgtttatt
    12841 cagaagagga tttgcttgcc aaatatagcc agcagttcaa ggttaaaaaa gtcatgccag
    12901 ctgccttcgc ggaacttcaa ggagattacc tgtatatgct gaccactccg agtcgacaag
    12961 aaatggagca gatgattgca gatttcggac aacgtcaaga ataagaacta tcggattaag
    13021 gaaattaact cccttaatcc ttttgttttg tctgataaaa aataagagca tcccaacaag
    13081 gtagctatca taaaacttgt tcgataacta tttgaagcag gattaggtgg tcagaaatta
    13141 aattttaata tttcaattga gtcatagtat tgtgtttgcg tatccttaaa tcagctaaaa
    13201 ggatccatga cgacacctat acgatatagt tttcaagata ccaaacaagt ctattaatat
    13261 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gtttctcaaa acactgtttt
    13321 gaggttgtgg atagaactga cagagtcagt atcatatacc tacggcaagg tgaagctgac
    13381 gtggtttgaa gagattttcg aagagtataa aatattcagg tgacgcatag atatagttaa
    13441 ttgaagcttt gtttgaaatc tgataaaata atgatattac taagttttaa aaactaaaga
    13501 aaagggaaga tatgattaca ggcgaattaa aaaataaaat cgatcagctg tgggaaattc
    13561 tttggacaga aggaaacgca aatcctttaa caaatattga acagttgact tatctcttat
    13621 ttatgaaaga tttggatagt gtcgagcttg gacgtgaaag tgatgctgaa tttctaggga
    13681 ttccttatga gggagttttt ccaaaagata aacctgaata ccgttggtca acttttaaaa
    13741 atataggaga tgctcaggaa gtttatcgtt taatgactca ggagattttt ccgtttatta
    13801 aaaatctcaa gggggataca gatgatacag ccttttcacg atatatgcga gaagctattt
    13861 ttcaaataaa taaacctgct acgcttcaaa aggcaatttc tatcttagat gtttttccaa
    13921 ctaggggatt agatgtagat tttgataatg acaaaaaaag tattactgat atcggagata
    13981 tctatgaata tctgttatca aaattgtcga ccgcaggtaa aaatggacag ttccgtacac
    14041 ctcgtcacat catcgatatg atggttgagt tgatgcaacc gactatcaaa gatatcatct
    14101 cagatcccgc tatgggttct gctggcttct tagtatctgc tagccgttac ttaaagcgta
    14161 agaaagatga atgggaaacc aatacagata atatcaatca ttttcataat cagatgtttc
    14221 atggaaatga tacggatacg actatgttga gacttggggc gatgaacatg atgctacatg
    14281 gagtagaaaa tccacaaatc agttaccttg actcgctgtc tcaagataat gaagaagccg
    14341 ataaatatac tttggtttta gcaaatcctc cttttaaggg ttcacttgac tacaattcaa
    14401 cttctaatga ccttcttgca accgtaaaaa ccaaaaaaac agaattactc tttctttctc
    14461 ttttcttgcg aactttaaaa ccaggtggac gagcagcagt tatcgtacct gatggtgtcc
    14521 tttttggttc gtctaaagct cataaaggaa ttcgtcagga aattgtagag aatcataagc
    14581 ttgatgctgt aatctcaatg cctagtggtg ttttcaagcc ttatgctgga gtttcaactg
    14641 ccattctcat ctttacaaaa actggtaatg gtggtactga caaagtctgg ttttacgata
    14701 tgaaagcgga tggtttaagt ttggatgata agcgacaacc gattagcgac aatgatattc
    14761 cagatattat cgaacgcttt catcatcttg aaaaagaagc agaacgtcag agaacggatc
    14821 aatctttctt tgttccagtt gctgagataa aggaaaatga ttatgatttg tctatcaata
    14881 aatataaaga gattgagtat gaaaaagttg agtatgaacc aacagaggtc atcttaaaga
    14941 aaatcaatga tttagaaaaa gaaattcaag ctggcttggc tgaattagaa aaattactca
    15001 agtagagagg tggctgtatg aaaaaagtga agttggggga agtcttatct ctaaaaaaag
    15061 gcaagaaagc cactgtactt gctgaacaaa caactctaag ccaacgttat attcaaatag
    15121 atgatttaag aaataataat aatttaaaat tcactgaaag tttaaatatg actgaagcac
    15181 tcccagatga tattctgata gcatgggatg gagctaatgc aggaacagtt ggttatggat
    15241 tatcgggagc tgttggtagt acaattacgg tcttaaaaaa gaatgagcga tacaaagaaa
    15301 aaattatatc agattacttg ggagtctttt tggaaagtaa atcg
//
LOCUS       DAYYQN010000047        14970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYYQN010000047 DAYYQN010000000
VERSION     DAYYQN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.047"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            424..2418
                     /gene="pspA"
                     /locus_tag="ACHBRQ_001521"
     CDS             424..2418
                     /gene="pspA"
                     /locus_tag="ACHBRQ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HFM9593163.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPAPKPAPAPAPTPEAPAPAPKPAPAPKPAPAPKPAPAPKPAPAPKPAPAPKP
                     APAPKPAPAPKPAPAPKPAPAPKPAPAPKPETPKTGWKQENGMWYFYNTDGSMATGWL
                     QNNGSWYYLNANGAMATGWAKVNGLWYYLNSNGAMATGWLEYNGSWYYLNANGAMATG
                     WLEYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWLEYNGSWYYLNSNGAMA
                     TGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGA
                     MKANQWFKVSDKWYYVNGLGALAVNTTVDGYTVNENGEWV"
     gene            2818..3939
                     /gene="mnmA"
                     /locus_tag="ACHBRQ_001522"
     CDS             2818..3939
                     /gene="mnmA"
                     /locus_tag="ACHBRQ_001522"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9593164.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTTLLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            4080..4533
                     /locus_tag="ACHBRQ_001523"
                     /pseudo
     CDS             4080..4533
                     /locus_tag="ACHBRQ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            4543..6456
                     /gene="mnmG"
                     /locus_tag="ACHBRQ_001524"
     CDS             4543..6456
                     /gene="mnmG"
                     /locus_tag="ACHBRQ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9593165.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDKILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDAVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            6660..6818
                     /locus_tag="ACHBRQ_001525"
     CDS             6660..6818
                     /locus_tag="ACHBRQ_001525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593166.1"
                     /translation="MKIKEQTRKLAVGCSKHCFEVVDRTDEVSSKHRFEVVDRTDEVS
                     NIYTARRS"
     gene            complement(6835..8514)
                     /locus_tag="ACHBRQ_001526"
     CDS             complement(6835..8514)
                     /locus_tag="ACHBRQ_001526"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9593167.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8516..8749)
                     /locus_tag="ACHBRQ_001527"
     CDS             complement(8516..8749)
                     /locus_tag="ACHBRQ_001527"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HFM9593168.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            9029..9242
                     /locus_tag="ACHBRQ_001528"
                     /pseudo
     CDS             9029..9242
                     /locus_tag="ACHBRQ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9565..9717)
                     /gene="cibB"
                     /locus_tag="ACHBRQ_001529"
     CDS             complement(9565..9717)
                     /gene="cibB"
                     /locus_tag="ACHBRQ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HFM9593169.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(9719..9904)
                     /gene="cibA"
                     /locus_tag="ACHBRQ_001530"
     CDS             complement(9719..9904)
                     /gene="cibA"
                     /locus_tag="ACHBRQ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HFM9593170.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            10082..10765
                     /gene="tsaB"
                     /locus_tag="ACHBRQ_001531"
     CDS             10082..10765
                     /gene="tsaB"
                     /locus_tag="ACHBRQ_001531"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9593171.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYVGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            10762..11199
                     /gene="rimI"
                     /locus_tag="ACHBRQ_001532"
     CDS             10762..11199
                     /gene="rimI"
                     /locus_tag="ACHBRQ_001532"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9593172.1"
                     /translation="MIEIKRIQKHSDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            11189..12199
                     /gene="tsaD"
                     /locus_tag="ACHBRQ_001533"
     CDS             11189..12199
                     /gene="tsaD"
                     /locus_tag="ACHBRQ_001533"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9593173.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(12239..13545)
                     /locus_tag="ACHBRQ_001534"
                     /pseudo
     CDS             complement(12239..13545)
                     /locus_tag="ACHBRQ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(13878..14572)
                     /locus_tag="ACHBRQ_001535"
                     /pseudo
     CDS             complement(13878..14572)
                     /locus_tag="ACHBRQ_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4464 a         2929 c         3455 g         4122 t
ORIGIN      
        1 aataaaagat ccgcagtttt aggtttacga agagacaaaa gcggagtatt taaatcagtt
       61 actctacatt ttacaagcgc taagaaatat gctaaagttc gtaaaacaga tgtgaaagaa
      121 atgaaatggc tataaagatt gtccgcaggc ttaagcttgc gttttttgtt gtttaacgaa
      181 attaaaaaac aagctctctc atcggaaatg tttttatagg aatgaaggaa gatgatgcta
      241 tgtttaaaaa gagaatcgaa aagggcaaaa gtagtatctt tatctatttt ttggagtaga
      301 agcttatgat atagaaattt gtaataaaaa tgtaatataa aacatttgac aaatatttac
      361 ggggggagta tacttaatat aagtatagtc taaaaatgac tatcagaaaa gaggtaaatt
      421 tagatgaata agaaaaaaat gattttaaca agtctagcca gcgtcgctat cttaggggct
      481 ggttttgttg cgtctcagcc tactgttgta agagcagaag aagctcccgt agctaagcag
      541 tctcaagctg agagagacta tgatgcagca atgaaaaaat ctgaagctgc taagaaagaa
      601 tacgaagaag ctaagaaaga tttagaagaa gcaaaagctg ctcagaaaaa atatgaagac
      661 gatcaaaaga aaactgaaga gaaagcaaag cttgttcaaa aagcggatga agagcgtcaa
      721 aaagcgaatg tggcagttca aaaggcgtat ttaaaactcc gagaagcaca agaacaattg
      781 aacaaaagtc caaacaataa gaaaaatgcc gctcaacaga aattaaaaga tgcattagct
      841 catattgatg aggtaacatt aaaacaaaaa gaggcggaag cgaatttcaa taaggaacaa
      901 gcaaaagtag ttcctgaagc agataagtta gctgagacta agaaaaaagc agaacaggct
      961 gaaaaaaaag aaccagaact tgctaaaaaa gtagcagaag ctaaagcaaa agcagaagaa
     1021 gctgagaaaa aagctgttga agccaaacaa aaagtggatg ctgaaaaata tgctcttgaa
     1081 gctaaaatcg ctgagttgga atatgaagtt cagggactag aaaaagagct caaagagatt
     1141 gatgagtctg actcagaaga ttatattaaa gaaggtctcc gtgctcctct tcaatctaaa
     1201 ttggatgcca aaaaagctaa actatcaaaa cttgaagagt tgagtgataa gattgatgag
     1261 ttagacgctg aaattgcaaa acttgaaaaa gatgtagaag atttcaaaaa ctcagacggt
     1321 gagcaagctg aacaatatct tgttgcagct aaaaaagact tagatgctaa aaaagctgaa
     1381 ttagaaaata ctgaagctga ccttaagaaa gcagttgatg agccagaaac tccagctccg
     1441 gctccggctc ctaaaccagc cccagctcca gctccaactc cagaagcccc agctccagct
     1501 cctaaaccag ctccggctcc taaaccagcc ccagctccta aaccagctcc ggctcctaaa
     1561 ccagctccgg ctcctaaacc agctccggct cctaaaccag ctccggctcc taaaccagct
     1621 ccggctccta aaccagctcc ggctcctaaa ccagctccag ctcctaaacc agctccagct
     1681 cctaaaccag aaactccaaa aacaggctgg aaacaagaaa atggtatgtg gtacttctac
     1741 aatacggatg gttcaatggc aacaggctgg ctacaaaata atggctcatg gtactacctc
     1801 aacgctaatg gtgctatggc aacaggttgg gctaaagtca acggcttatg gtattacctc
     1861 aacagcaatg gtgctatggc gacaggttgg ctagaataca atggttcatg gtactacctc
     1921 aacgctaatg gtgctatggc gacaggttgg ctagaataca atggctcatg gtactacctc
     1981 aacagcaatg gcgctatggc gacaggttgg ctccaataca atggttcatg gtactacctc
     2041 aacgctaatg gtgctatggc gacaggttgg ctagaataca atggctcatg gtactacctc
     2101 aacagcaatg gcgctatggc gacaggttgg ctccaataca atggttcatg gtactatcta
     2161 aacgctaacg gcgctatggc aacaggttgg gctaaagtca acggttcatg gtactacctc
     2221 aacgctaacg gttcaatggc aacaggttgg gtgaaagatg gagatacctg gtactatctt
     2281 gaagcatcag gtgctatgaa agcaaaccaa tggttcaaag tatcagataa atggtactat
     2341 gtcaatggct taggtgccct tgcagtcaac acaactgtag acggatatac tgtcaatgaa
     2401 aacggtgaat gggtttaagc cgattaaatt aaatcatgtt aagaacattt gacattttaa
     2461 ttttgaaaca aagataaggt tcgattgaat agatttatgt tcgtattctt taggtacctg
     2521 tcttatgatt tcaggaaatg tcattaaaaa aacgactcct tttctctaac ttggaaaata
     2581 gattagagaa aatgggttgt tttatttatt atagttattt gaatgaagat aagaagaagg
     2641 tatactcaca tcattcacaa atctgtatat tgactataag ttttaaaaaa caatttttaa
     2701 gctcttcctt gtcttctcta accaagcgtg ttataatgaa tactgctcaa gcgaccttca
     2761 atcgtgaagc acacacgacc ttcaatcgtg aataaacgaa tagatgggag acttaccatg
     2821 agtgataact ctaaaacacg tgttgtcgtg gggatgagtg gtggtgttga ttcgtcggtg
     2881 acgactcttc ttctcaagga gcagggctac gatgtgatcg gtatcttcat gaagaactgg
     2941 gatgacacag atgaaaacgg cgtctgtacg gcgaccgaag attacaagga tgtggttgcg
     3001 gtggcagacc agattggcat tccctactac tctgtcaatt ttgaaaaaga gtactgggac
     3061 cgcgtttttg agtatttcct agcggaatac cgtgcgggac gcacgccaaa tccggacgtt
     3121 atgtgcaaca aggaaatcaa gttcaaggcc tttttggact atgctatgac cttgggggca
     3181 gactatgtag cgactgggca ttatgctcga gtggtgcgtg atgaggatgg cactgttcac
     3241 atgcttcgtg gcgtggacaa tggcaaggac cagacctatt tcctcagcca actttcgcaa
     3301 gaacaacttc aaaaaaccat gttcccacta ggacatttga aaaagcctga agttcgaaaa
     3361 ctagcagaag aagcaggtct ttcgactgct aagaagaaag actcgacagg gatttgcttt
     3421 atcggagaaa agaactttaa aaacttcctc agcaactacc tgccagctca gcctggtcgc
     3481 atgatgactg tggatggtcg cgatatgggc gagcatgcag gtcttatgta ctatacgatt
     3541 ggtcagcgtg gcgggcttgg tatcggtgga caacacggtg gtgacaacgc gccgtggttc
     3601 gttgtcggaa aagacctgag caagaatatt ctctatgtag gccaaggttt ctaccatgat
     3661 tcgctcatgt caactagcct agaggctagc caagtccact ttactcgtga tatgcccgag
     3721 gagtttacgc tagaatgtac ggctaaattc cgttaccgtc agcctgactc taaggtgacc
     3781 gtccatgtca aaggagacaa ggcagaagtt atctttgcgg aaccacaacg cgcgattaca
     3841 ccaggacagg cagttgtctt ttacgatggc gaagagtgtc taggtggcgg tttgattgac
     3901 aatgcttacc gcgatggaca agtttgtcag tacatttaga ttgacaaatt ttctcaattt
     3961 gctacaataa taacagcaat agaaatgatg gtcaaagctc atggatgttg caggcttttt
     4021 tgtcctgcac ttctttggag ttttgactgt ttttgtgtcg tttaagggaa aggacaagaa
     4081 tgactcaaca agactttcgg acaaaagtag acaatacggt ttttggtgtt cgggcgacag
     4141 ctttgattct tcaaaatcgc aagcttctag tcaccaaaga caagggcaag tattacacta
     4201 tcggcggtgc gattcaagtt aacgaaaaaa cggaagacgc ggtagtccgt gaagtgaagg
     4261 aagaactggg tgtcaaagct caagctgggc agctagcttt tgtggttgaa aatcgttttg
     4321 aacaggacgg tttttcctat cacaatatcg aatttcatta tctggtggac ttgctggagg
     4381 atgccccatt gaccatgcag gaagatgaga aaaggcagcc ctgtgagtgg attgacttgg
     4441 ataagtgagg atatccagct agttccagcc tttttaaaaa cggccctacc agattgggaa
     4501 ggccaactaa gacacattca tcgtgaggaa taggagagaa acatgactta tcattttact
     4561 gaagaatacg atattattgt aattggtgcg ggacacgctg gggttgaggc ttccttggcc
     4621 gctagccgta tgggctgtaa ggttctactt gcgaccatca atattgaaat gctggctttc
     4681 atgccttgta atccctctat cggtggttct gctaagggga ttgtcgtacg tgaagtcgat
     4741 gccctcggtg gcgagatggc caagaccatt gacaagactt acatccagat gaagatgctc
     4801 aacacaggga agggtcctgc tgttcgtgcc cttcgtgcgc aggctgacaa ggaactttac
     4861 tctaaggaga tgcgcaagac agttgaaaat caagaaaatc tgacccttcg tcaaaccatg
     4921 attgataaga ttttggtgga agatggcaag gttgtcggtg ttcgtacagc gactcatcaa
     4981 gagtatgctg ctaaggctgt tattgtgacg acagggactg ctctccgtgg ggaaattatc
     5041 atcggagacc tcaagtactc atcaggtcct aaccacagct tggcttctat taacctagct
     5101 gacaatctca aggaactggg tctcgaaatc ggtcgtttca agacaggaac ccctccacgt
     5161 gtcaaggcct cttctatcaa ctacgacgtg acagaaatcc aaccaggaga cgcagtacct
     5221 aatcatttct catacacttc acgtgatgag gattatgtca aagatcaagt gccatgctgg
     5281 ttgacctata ccaatggtac cagtcatgag attattcaaa acaacctcca ccgtgcgcct
     5341 atgtttacag gtgtggtcaa gggagtgggg cctcgttact gtccgtcgat tgaagacaag
     5401 attgtgcgct ttgcggacaa ggaacgtcac caactcttcc ttgagccaga agggcgcaat
     5461 actgaggaag tctatgttca aggactttca accagtctgc ctgaggatgt ccagcgtgac
     5521 ttggttcatt ccatcaaagg tttggaaaat gcagagatga tgcggacagg ttatgctatt
     5581 gagtatgata tggtcttgcc tcatcagttg cgtgcgactt tggaaaccaa gaaaatctca
     5641 ggtctcttca ctgctggtca gacaaatgga acgtcaggtt acgaagaggc agcaggccaa
     5701 gggattatcg caggtatcaa tgcggctcta aaaatccaag gcaagcctga attgattcta
     5761 aaacgaagtg acggttatat cggggtgatg atcgacgact tggtgaccaa gggaaccatt
     5821 gaaccttacc gtctcttgac cagtcgtgct gaatatcgtc tcattcttcg ccatgacaat
     5881 gctgatatgc gcttgactga gatgggccgt gagattggcc ttgtggacga tgaacgctgg
     5941 gctcgttttg aaatcaagaa aaatcaattt gataatgaga tgaaacgcct agacagtatc
     6001 aaactcaagc cagtcaagga aaccaatgct aaggttgagg aaatgggctt caagccgttg
     6061 acagatgcgg tgacagccaa ggaattcctt cgccgtccag aagtttctta ccaagatgtg
     6121 gtggccttca tcggaccagc tgcagaggaa ttagatgaca agattatcga attgattgaa
     6181 acagaaatca agtacgaagg ctatatttcc aaagccatgg atcaggttgc caagatgaaa
     6241 cgtatggaag aaaaacgcat tccagccaat atcgactggg atgacatcga ttctattgcg
     6301 acggaagctc gtcagaagtt caaactcatc aatccagaaa ccatcggcca agccagccgt
     6361 atttcgggag taaacccagc agatatttct attttgatgg tgtatctgga aggtaaaaat
     6421 cgtagtattt ctaaaactct tcaaaaatca aaatgatacg tcgtaggctt cttacgaatg
     6481 agttcaaagc ttggctttga ttcatctcca acctcccata gttccccgaa ctatgggagc
     6541 taactcaagt tatgcatttt cctagccttc tggtctgatt ttcattgagt tttaatctaa
     6601 aaagaaagca tagagaaaaa cagctccgaa gacggggctg ttttgttgat ttgtactcaa
     6661 tgaaaatcaa agagcaaact aggaagctag ccgtaggttg ctcaaagcac tgctttgagg
     6721 ttgtggatag aactgacgaa gtcagctcaa aacaccgttt tgaggttgtg gatagaactg
     6781 acgaagtcag taacatatat acggcaaggc gaagctgacg tggtttgaag agtattattc
     6841 ttcatctggt gtgaggatca tcgggatgat gatcggttca cgttcggtat tttcatagag
     6901 gaaggggcga atagcgttga caatggcacc attgacagat tgcacgctag catccttatt
     6961 tttcagtgcg atacgaatgg cattgaagag gatacgctgg ctttgacgaa tcaagtcgcc
     7021 agactctctc atgtagacaa agcctcggct gaggatgtct ggaccagata gaatcatctg
     7081 cgatttgaag tcaacagttg cgactgccag aacgacaccg tcttcagata gatcgcgacg
     7141 atctttgagg acagctgcgc caatttcacc gatacgattt ccatcgacat agatatcttg
     7201 ggcgttgaaa tggcctgcga tacgagctga gtcagcagta agggcaagca catcgccatt
     7261 gctcatgata aagatattgt ccttctcaac accagtatcc actgctagtc cagcgtggac
     7321 tttttgcatg cggtattcac cgtggacagg catgaagtat tttggcttaa tcaagcagag
     7381 catgagtttt tgctcttgct gaccaccgtg tccagatgta tggatattgt tcactttacc
     7441 gtggataact tcgacaccag cttcagaaat gatgttaatc agcttgttga cgctagtagt
     7501 gtttccaggg atgggactag aagagaagat aacggtatca cctggttgta attgtacttg
     7561 acggtgggtt ccgttggcga tacgagagag ggctgccata ggctcaccct gactacctgt
     7621 acagaggata agaacttctc ctgcaggata atctttgatt tcatttggct cgataaaggt
     7681 tcccttagga gctttgatgt agccaagatc gattccgttg acaatggcct tttccataga
     7741 acgaccaaag accgcaatct tgcgtccagt cttaacagca gcttctgttg cctgctggag
     7801 acggaagata tttgaggcaa aggatgcaaa gatgatacgt ccttcaatac cttggataat
     7861 cttcataatg gactgaccaa cgactttttc agagttggta aaggttggta cttccgcatt
     7921 tgtcgagtca gacaggagac agagcacgcc ttcttcacca agcgcagcca tacgatgcaa
     7981 gtccgcaggt tctccaactg gagtaaagtc gaacttaaag tcacccgtac agacgatttt
     8041 cccttgagga gtatgaatga caatccccaa aggctctgga atagagtgag tcgttctaaa
     8101 gaaagttgcc ttgagatttt taaaggtcaa ctcggtgttg tggttgattt cgtaaagttt
     8161 ggcgttgcgc aagaggccgt gttcttcgag tttcccacgg atcaaagcca aggcaagcgg
     8221 tccagcataa atagggacat ttgcttgctt gagtaggaac ggaatcccac caatgtggtc
     8281 ctcgtgtccg tgtgtgatta aaacagcctt gacgcggtcg atattgtcta cgatgtaaga
     8341 gtagtcagga atgacatagt cgataccaag caagtcatct tctgggaatt taatcccagc
     8401 atcgacgatg ataatctcgc cttggtattc aattccgtaa gtgtttttcc cgatttctcc
     8461 tagaccaccg atggcaaaaa cgccgacttc ttcaggttta agagtgtagg ccatattaga
     8521 actccgtaat ttcgaaggcg ccagtttctt tttcgtaatc gagcaatttg tcagacaaga
     8581 gttcgatata ttcgatattg tactctgggc gattttcttc gacaagttgg cgagcagtga
     8641 tacggccctc aagttctgag ctggcatcga tgtctaggta aagcgcgcgt gttgtttcac
     8701 ggcgtgggct acgttctttt gtttcttgat aaaaaacttt gtaaatcata tagttccttt
     8761 ctatttacag gtcagcttat tccaaacttt tagcagatat ttgcttgatt tcattccatt
     8821 ttgtgtctag attgaccttg attcgttaca attatttcaa ttataccaca aaatgaaaaa
     8881 ttagtaaaag acggaaacga gaaagttagg ggacgatttc ttcggtaagc gtttgcgaaa
     8941 ataaagaaaa tgtattatgt tgataacgga agtgaaattt attgcttgtc ataattccat
     9001 atagatttta agtcaaggag gttgccctat gtataactta tttgcttttg ctatcggagt
     9061 tcgtttgcta gcttttatcg tttttgtttt gcgtttatgg aggaaatctt ctatttgcca
     9121 aactggaacg atgacaaacc aagttcttga ggaaaattac ctttgtgact ctctactcgc
     9181 tttttctcct cctcgtgacc tatgtcgttt ggtttgtatt ttactttgga ttaaatgctt
     9241 agatgccctg cttatgctgg ggttttttgt ttggggatgt agagatagta gatttacgac
     9301 acgaaaattc caccccatct atcaagatga ggtggtcttt gcatttttct aaaatgatat
     9361 gacaatggaa ttttcaaagc ttattatttt tatgggagtc attgttctgc tcaaaaatag
     9421 agttgaagca taaaaataaa acaatacaaa cagataaatc gaatggtaaa gggatggtag
     9481 taatgttcaa atcattgaga atgctgagca aactgtaaga gatgaagaca ataaaattta
     9541 tccttctaaa aatgtgtttc ttatctagca tgacttacca aactttttac gaaggtcttt
     9601 tccaatggta taacctgcca taaataaagc gataccaaca cctacaccaa agccaccctt
     9661 gatttcttgc aattccttat tagaaatttc acgacagttg ttcaaatctt tcatcatatt
     9721 aatctcctcc taaaaaataa ccagctgcca aaccagaacc tataagtgct gttccacctg
     9781 caatagcttt ccaagatact gctactccaa agataaccaa gggagcgaga ccgccatcaa
     9841 tatctgataa ttcattttca gataaggata aaaattgatt gtcaagaatg tcaaaatttg
     9901 tcattgaact ttcctccttt tttcttgttc acttttatat tcggaaaaga aaaggaaaag
     9961 tgagcatttt tttaaattat ttttagttaa gtgagggcac tcttgtctgg gggcaaagaa
    10021 aattcccatg tgccagcttt tgtagtataa tagtaagcag acaaaaagat aggaatgtgt
    10081 tatgaaagta ttagcttttg atacgtccag caaggctctt tctctcgcta ttttagagga
    10141 caagcaggtt cttgccgaga cgacgattaa tattaagaaa aatcacagta ttactcttat
    10201 gcctgccata gattttttga tggcaagttt ggattggaca cccaaggatt tggaccgaat
    10261 cgtggtagct gaagggccgg gtagctacac aggcttgcga attgcggtag ccactgccaa
    10321 gactttggct catactctga acatcgagtt agttggtatg tctagcctct tggctctggt
    10381 accctaccaa caagaaggct tgttcgtctc tttgatggat gcgcgtcgca acaatgttta
    10441 tgtaggattt tatgaaaatg ctaaacccgt catgccagaa gctcacttac cctttgagcg
    10501 agtgattgag ttaatcaagg gtgctagtca ggttaccttt gtcggagaag ttggcccctt
    10561 tgttgagcag attcaagaat acttgccaag gactaatttc aaagaaacct tgccaaatgc
    10621 agctaatcta gctctatggg cctgggacaa ggaagcagac tccttgcatg attttgtgcc
    10681 gaactacctc aagcgtgtcg aagctgagga aaactggctc aagaatcaca ccgagtctgg
    10741 cgagtcttac attaaacgct tatgatagaa atcaaacgaa tccaaaaaca ttctgacttg
    10801 gctcaagcca tctacgctgt tatgatagct gtttacccag tcagtccttg gacgctggaa
    10861 caaatccaag cagacctgtc ccaagaccaa acttggtatg ctttggctta tgatggggca
    10921 gaagtgattg gatttctagc tgtgcaggag aatctttttg aagcagaagt cctgcaaatc
    10981 gctgtcaaag gagcttatca gggtcaggga attgcgtcag ccttgtttgc tcaattgccg
    11041 acagacaagg aaattttcct cgaagtcaga cagtcaaatc aacgagcgca agcattttac
    11101 aagaaagaaa agatggcagt tatcgctgag cgaaaggcct actaccatga cccagtcgag
    11161 gacgccatta tcatgaagag agaaatagat gaaggataga tatattttag catttgagac
    11221 atcctgtgat gagaccagtg tcgccgtctt gaaaaacgac gatgagctct tgtccaatgt
    11281 cattgctagt caaattgaga gtcacaaacg ttttggtggc gtagtgcccg aagtagccag
    11341 tcgtcaccat gtcgaggtca ttacagcctg tatcgaggag gcattggcag aagcagggat
    11401 taccgaagag gacgtgacag ctgttgcggt tacctacgga ccaggcttgg tcggagcctt
    11461 gctagttggt ttgtcagctg ccaaggcctt tgcttgggct cacggacttc cactgattcc
    11521 tgttaatcac atggctgggc acctcatggc agctcagagt gtggagcctt tggagtttcc
    11581 cttgctagcc cttttagtca gtggtgggca cacagagttg gtctatgttt ctgaggctgg
    11641 cgattacaag attgttgggg agacacgaga cgatgcagtt ggggaggctt atgacaaggt
    11701 cggtcgtgtc atgggcttga cctatcctgc aggtcgtgag attgacgagc tggctcatca
    11761 ggggcaggat atttatgatt tcccccgtgc catgattaag gaagataatc tggagttctc
    11821 cttctcaggt ttgaaatctg cctttatcaa tcttcatcac aatgccgagc aaaagggaga
    11881 aagcctgtct acagaagatt tgtgtgcttc cttccaagca gcagttatgg acattctcat
    11941 ggcaaaaacc aagaaggctt tggagaaata tcctgttaaa accctagttg tggcaggtgg
    12001 tgtggcagcc aataaaggtc tcagagaacg cctagcagcc gaaatcacag atgtcaaggt
    12061 tatcatcccc cctctgcgac tctgcggaga caatgcaggt atgattgcct atgccagcgt
    12121 cagcgagtgg aacaaagaaa acttcgcagg ctgggacctc aatgccaaac caagtcttgc
    12181 ctttgatacc atggaataaa gagtggctct ttgtcaagtg tagtgggtag gcgaaaagct
    12241 acaatctgga gagtacgaaa ttcgtactct cttttttgat gttcaaagtc atcaaaatcc
    12301 gttttttaaa attgttaaag ttccgaaaac caaaggcttt gcgcttgata accttaataa
    12361 aattattggt cgcttcaagt ttagcgttgg aataaggcag cttaagtgcg ttgataatct
    12421 tatctctatc ccttaaaaaa gtccaaaaga ccgtttgaaa aagcggatga accgttggca
    12481 gttcttgctc aatcaatcca aagaaatggt tggcattctt ctcttgaaaa tgaaagagca
    12541 ggagttgata gagttcgtag tgaacctgta atccctcaga gtaagaaagc agcttatctc
    12601 gtacttcccg atgggttaag tgcattcgaa acgtgggctg atagaatcgt ttctgacaga
    12661 gcttgaagct atcctggtgt agtaaatcag tccagtggac tgatttagcc cgagcctaga
    12721 aacgaaaaag cgagggttcc aaaagcgctt gagcgcccga tactccaagg attttcggtc
    12781 aaattggttc atgatttgga ttcgtactcg gttcatagct cggctcagat gttggacaat
    12841 gtggaggcgg tcaagaacaa tcttcgcctt tggaaagagt tgcttagcga gccgataata
    12901 agggctgtac atgtccatgg tgatgacctc gaccagctcc cgaacctctc tctgatagcg
    12961 ttggaaatgg tttcgaatca ccgcatgagt tcgcccgtct aaaattgcga ggatggattt
    13021 ggactcaaaa tcttgggcaa tgaagctcat tttgctcttt ttgaagctat actcatccca
    13081 actcataact tttggcaact tggtccaatc ggtttcaaac ttaaattccc tcagttttcg
    13141 gatgacggtg gaagttgaga ccgccaatct gtgggcaatc tcagtcatag actgcttttc
    13201 aaggaggagc tgagcgattt tttgtctcac catgttggaa atctggcaat ttttcttgac
    13261 aatggatgtt tgagaaacga ccaccttaag gcaattcttg cactgaaagc ggcgcttttt
    13321 gagatgcagt accgtgggta aaacctgaca gtcgagaagc ggaattttag aggctttctg
    13381 gaagtcgtat ttgatcatct tcccttgaca atgaggacaa ggaggagcgg gggaatccaa
    13441 ctttgcctga acgactagat gggtttggta tttcagaaca gacaagattt tgatattttt
    13501 gtcttccaat ccgagcaaat ctgtggtatt cttaagttgt tccatatgaa ccctttctaa
    13561 tgagttggtt agacgctttt cattataggt catatggggc tttttttcta caagaaacga
    13621 ccctataatt cctggggtgg gattacccac tacagaaatt atagagccaa agcattccaa
    13681 agtcttgtct gactctggaa tgttttctgt tcacagttgt tttgtaactg cctaataagt
    13741 aaccgcgcta taacgaggta tctaccgaga ttgtcaattc tccagtatac tgttataaaa
    13801 aacaaaactg gaggattttt ttatgtcccc ccatcgtccc atcgactgtt ccgtagctgc
    13861 ccaataacgg agtataatca gctgaaacaa gaataggaca aaagtgcctc aagaaaagca
    13921 tcgtaatttg acaatattat tctgagatgt ttttgatgta agcccatatt ttctcaatgg
    13981 gattatactc gggtgaatag ataggaagtg gtaacagtct atgccctgct ccttacacat
    14041 gttcattctg tgaaaccttg cattgtccat aatgataagg gatggtgtat ctaaagtagg
    14101 tagtaagaat ttttggaagc aagcttcgaa aaagtcactc gtcatcgtat ctttataggt
    14161 catcggagcc atagtatatt gaaacaaaaa agggcattag tacttctata acagtatcgc
    14221 aatctctggt gtgcgttgtc tcgatcttaa ttcaaataat tatatcttct tccagagaga
    14281 ctaactgcct gctcaaggct cgatcatatt cgagataaaa ataggtctta acccctgtct
    14341 cataaatata aataggagtc aggtagctta agtgattcaa ttctttaagg aaccgattta
    14401 ctttttctgg gtcttgttcg cagtaggtac ggctcttttt tagactatat cccatagcct
    14461 ttagagcgta atgaatagtt gttggaggac agttgaattc agcagctatt tcagtcaaat
    14521 aagcatcggg atgagtttca atatagatct ttaatttatc tctatcaact ttttcaatta
    14581 atagtggaag ttttcttaaa gttagtgtat tgaaacgttt ttatacagtg ctaaaatttg
    14641 aagtgtagtg cagatatatt tgtaatagta ttatagaaat tctttgaaga aggtcgagac
    14701 aagagaaaat aggtatcttt gagatataag catgatttac tataaaatta ttgagttatt
    14761 cataaaaatt tctctatcaa atatcataat gcgtcgttta aggagatggt tacaatgcaa
    14821 tatagttgtg gaaaaataaa tattaatatc cctgatggat atggagacat taaggatata
    14881 gtgttttcgg ctcatattat agttaggtat aataatggac attgtggagg tatagatccg
    14941 catataattg gactttgtaa aaagcagatc
//
LOCUS       DAYYQN010000048        14680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYYQN010000048 DAYYQN010000000
VERSION     DAYYQN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.048"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            74..530
                     /locus_tag="ACHBRQ_001536"
                     /pseudo
     CDS             74..530
                     /locus_tag="ACHBRQ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            695..994
                     /locus_tag="ACHBRQ_001537"
     CDS             695..994
                     /locus_tag="ACHBRQ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9593174.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1255..1578
                     /gene="tnpB"
                     /locus_tag="ACHBRQ_001538"
     CDS             1255..1578
                     /gene="tnpB"
                     /locus_tag="ACHBRQ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9593175.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(2307..4034)
                     /gene="ezrA"
                     /locus_tag="ACHBRQ_001539"
     CDS             complement(2307..4034)
                     /gene="ezrA"
                     /locus_tag="ACHBRQ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9593176.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(4116..6062)
                     /gene="gyrB"
                     /locus_tag="ACHBRQ_001540"
     CDS             complement(4116..6062)
                     /gene="gyrB"
                     /locus_tag="ACHBRQ_001540"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9593177.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(6077..6640)
                     /locus_tag="ACHBRQ_001541"
     CDS             complement(6077..6640)
                     /locus_tag="ACHBRQ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9593178.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(6725..7279)
                     /locus_tag="ACHBRQ_001542"
     CDS             complement(6725..7279)
                     /locus_tag="ACHBRQ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HFM9593179.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(7290..8513)
                     /locus_tag="ACHBRQ_001543"
     CDS             complement(7290..8513)
                     /locus_tag="ACHBRQ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9593180.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(8550..11000)
                     /locus_tag="ACHBRQ_001544"
     CDS             complement(8550..11000)
                     /locus_tag="ACHBRQ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HFM9593181.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(11200..12129)
                     /locus_tag="ACHBRQ_001545"
     CDS             complement(11200..12129)
                     /locus_tag="ACHBRQ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9593182.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            12269..12556
                     /locus_tag="ACHBRQ_001546"
     CDS             12269..12556
                     /locus_tag="ACHBRQ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HFM9593183.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(12590..13924)
                     /gene="ciaH"
                     /locus_tag="ACHBRQ_001547"
     CDS             complement(12590..13924)
                     /gene="ciaH"
                     /locus_tag="ACHBRQ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HFM9593184.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(13914..14588)
                     /gene="ciaR"
                     /locus_tag="ACHBRQ_001548"
     CDS             complement(13914..14588)
                     /gene="ciaR"
                     /locus_tag="ACHBRQ_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HFM9593185.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         4514 a         3089 c         2606 g         4471 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttca ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaaagcg aatacagact
      121 atattgctcg ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc
      181 ttgttcaaga accaaatggt gcttggttcc taccatgtgg agaaattgaa gcaggtgaaa
      241 atcatcagga agccctaaag cgtgagttga ttgaagagct tggtttcaca gcagaaattg
      301 gtacctatta cggacaagct gacgaatatt tctattctcg tcatcgtgat acctactact
      361 acaatcctgc ctacctctat gaagcaactc ctttcaaaga agtacaaaag ccactagaaa
      421 actttaatca tattgcctgg ttccctattg acgaggctat caaaaacctt aaacgtggta
      481 gccataaatg ggccattgaa tcttggaaaa aacagcataa gattgactaa tactcttcga
      541 aaataaaatt caaaccacat cagcgtcgcc ttaccatact caagtatagc ctgcggctag
      601 cttcctagtt tgctctttta ttttcattga gtataaatca agcgacttta ttcaaaaagt
      661 gctataatag aattagaaaa tcggaggaaa tattatgaag cttatcaata cgacaaactc
      721 acactcgcaa cttgtaaaaa gtcagctaga aagtaccgat gcaacccttg ttgaggttta
      781 ttctgcaggg aatacagatg ttatttttac ccaagctccg cttcactatg aaatcctcat
      841 ctcaaacaaa caccgtgcta ttcgtgagcc tgaaatcgaa accattcaag aatttttctt
      901 gaaacgtaaa attgataagg cctctgttga tgaagccaat atcaagacac tctactcaga
      961 gaaattaatt ggaatttcaa tcccaatcaa gtaagaaaat tgagtaaaaa ctcgatttga
     1021 gagaaagtcc aataagtctt tccataataa aacgcatagt agcaagagtt tctacaccca
     1081 ctattatacg tttttatcat tttgaaatct gctgctaatc aatgaagaag caaagatcaa
     1141 actaggcagc tagttacaag ttacgtaagc gcgtcataac aaggtatcta tcctaattcc
     1201 ccaacttcca gtatactaga taaaaaataa ttatcggagg aaaagtattg tactatgatg
     1261 attcaactaa gtgatttagg tcaagttcac cttgtttgtg gcaagacaga tatgatacaa
     1321 ggaatcgatt cactggctta tctcgttaaa acccactttg aattggatcc tttctccggt
     1381 caagtctttc tcttttgtgg tggacgtaaa gaccgcttta aagcccttta ctgggatggt
     1441 caaggatttt ggatactata taaacgcttt gagaacggaa aactgacttg gcccagtaca
     1501 gaaaaggatg tcaaagctct cgcacctgaa caagtagact ggcttatgaa gggcttttct
     1561 atcactcccc aaatatagtg gatttaaact agaatagtac acccctactt ctaaaacatt
     1621 gttagaaatc gatttgactg tcctgatcaa tttgtcctgt tcttgtttca ttttactata
     1681 gaacgatttg aaggcgttta tgatatttag ctgtacgaga gtcttttaaa agtgttttga
     1741 tggtttggat ttcttcttta gttgatttta tattactatt atataatgat tttttatttt
     1801 agtctagtat aaatagcgag aaatatctat cctatcttct agaatgtctt ccaaacgagg
     1861 aaactctcgt aaacaaagag gttttagagg cctatttacc gtggactaaa gttgtacaag
     1921 aaaagtgcaa ataagaaatc tccagattag gaactatccg tgagttctct agtctggaga
     1981 tttttcaata gacttcgtta ttggacggtt accaagttac tcaaagtaca gatttgaagc
     2041 tgcagataag actgacgaaa tcactttaat actaaatgaa aatcaaaaag caaactaaga
     2101 agctagcctc aggctgtact tgagtacggt aaggcgacgc tgacgtgatt tgaagagatt
     2161 ttcgaagagt ataaaacact gttttgaggt ggtggataaa tcggctgggt aaaaactcaa
     2221 tctcaggaga aaatgaagta aatctcccca caactataga aaaagatttg attctgctcc
     2281 tcacacaata aaatcttttt cttttattaa aaacgaatcg tttcacgtgt tttctcatat
     2341 gaggtaacaa agcgattggt tacaccaggc tctgccactt ccaatgcttg agaaatcttg
     2401 tcaaatgaag cgtgataatc aaattctttt tcaaaaatat ctaaagcttc gttaaatgct
     2461 tcttgaatgc gttcatcaaa tgagcgatag cggttagaat attgcaagag ttgctctgtc
     2521 aaagttgcat attgtacaat attataagtt tccgtttcta aagcttccat atcattcgtt
     2581 gcaatttcaa gaactcgggt aacagattca atgttaatca ttttttgttc taactcaacc
     2641 attaaatcct cggtattatt gcttgccgta aagaataact tcaagaaagt ttgtggaata
     2701 cctggcagat tgcgtttttc catgtatcgc ttgatagtat ggagacgatt gacataaaca
     2761 ttggcctttt gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg ctcactaact
     2821 gaaatttgct catcttcaat atcttttagt tgagtttgta aatcctcaag attttcttca
     2881 agaactgaat aagcttgggt tggttcttct tgatttgaag ttacctcaac aatagctgcc
     2941 tcaaaactct ctaattctgt ctgaatacga cgaacatggc ttgcagctgt ctcaggaagt
     3001 aaataggtct tgttcaaacg tgcaatatct tctcccaata aagtattatt ctctttcata
     3061 tgttgaaggt aagttggaag agttgcaagt agattttcca ctactttctg agcagcaatt
     3121 tctcgagtaa aaatatcata caaggcattg atttcctctt gtgcctgtcc attctcatat
     3181 tcggcattat ccaattccaa ctgacgaata ttctcttggt ttttcttgaa tgcttcataa
     3241 agcaagtgga aacgcgcttc aatatccgtt tcaacaaaat gataattagc atcaattagt
     3301 ttacgataac cggattccaa atcctgtaat tgatctggca attctgtaga aagcgtcgta
     3361 accaaggctg gaacacgatc cacaatatga cttaaggcca aaatgtgatt ttctgtatta
     3421 tccaaaatca ctgcggcttc cacagggtca cccgatgaat tcaaggttac aaattgtgaa
     3481 aattcagatt ggatattttc taattgtttt tcaatttcat ccaaggcttg accatactgt
     3541 tctgaatttt cagcaactct atgctgaagt tcctcaaata aatccaaagc atgaagaaca
     3601 cgaccactat ttttagattc ttgcttctct aagtctgcca aagcattgcg aattgccgca
     3661 atatcttcgt caatcaaagt aatttgactc tcaatttggt caatttgatg actggccttg
     3721 agaaaacgaa atgaatggtt atagccttct gcttcaaaga gattattttc aatatcggca
     3781 aaagagttga gagataaatc gacccatttt tgattccatt cacggaaagc cacttgactt
     3841 tgtccaatca agtgcatatt ttttacagct tctacttcat catttactgg aagattgtat
     3901 agttcttctt ttctttcttc tagcgcctct aatctcccct cgtttcgctt acgtagaaag
     3961 attgccacta cataagccag aactaaaatg actgcaattg caaccattaa ataaattagt
     4021 tgtccattag acatatcaaa ctcctttttt acttgaaaca atcgtaacga ttatatcata
     4081 ttttttagac caagggaact acttctccct attttttaga catcaagtgt actatagaca
     4141 gcattttctt cgataaactc acgacgaggc tctactcgat cccccatcaa catatcaaag
     4201 attttatctg cttctgcagc atcatctaca gaaactctag ccatcaagcg atgttcggga
     4261 tccatggttg tttcccacag ctgatgatcg tccatttcac ctagcccctt ataacgctga
     4321 atagtcggtt tggtacgacc ttcactataa cgggctaaag cttcttggag tttgatttct
     4381 tgatctgcac ccggctggat atattcttta atctcgcttc caaccttgac accatagatt
     4441 ggtggttggg caatataaac ataaccagct tctaggattg gtttcatata acgataaatc
     4501 aaggttaaaa gaagggtacg aatgtgggct ccatcgacat cggcatcggt catcaaaacg
     4561 agtttttggt aacgggcttt cgaaacatca aattctgcgc caaatcctgt tcccatggct
     4621 gtgaaaagac tacgaatttc ttcgttagct agaatcttat ccatacttgc tttttcaacg
     4681 ttcaaaatct taccgcgaat tggaaggata gcctgaaact cacggttacg accagatttg
     4741 gctgatccac cagctgagtc tccttcgacg atgaagagtt ctgtttcagc agggttatta
     4801 gaagaacagt ctgctagttt ccctggaagg ttggaaattt ccaaaccaga ttttttacgt
     4861 gtgacttcac gcgcacgctt ggcagccaca cgagccttgg cagctaaaat ccctttttcc
     4921 acgatacgtt tggcaatctg tggattttcc atgaggaaat cggagaaggc ttcactgaag
     4981 aggcgattgg taatcttgac cacttcgcta tttcccaatt tggtcttggt ttgtccttca
     5041 aactgtggat ttgggtgttt aactgagata actgcagtta atccttcgcg aacatcttcc
     5101 cctgttaaat tatcttcatt gtctttcagt aacttattct tacgagcata atcattgata
     5161 acacgagtca gtgccgtacg gaaaccttgt tcatgcgttc caccttcatg ggtatgaata
     5221 ttattggcga aactcatgac attttcatgg taacctgttg tgtactgcat ggctacctca
     5281 actgtgatat catccatctc accgtctgta tagattggtg tatcaaagat tacatccttg
     5341 ttctcgttga tatattcaac gtaactagca atcccacctt cataatgata atgcttggtt
     5401 tgttccaaac cttggcgctt atctgtaatt gaaatttgaa gaccgcgatt tagaaaggcc
     5461 aactcttgaa tccgtttatt taatttatca aaatcaaaga ttgttgtttc agtgaaaatt
     5521 tctgggtccg gtgtgaagtg aaccgttgtt ccagttctat ctgtatcccc aaccacctca
     5581 aggtctgcaa caacatgacc acgacggtat tcttggtaat gaatcttacc gtttttatgg
     5641 acatgaacgt ctaattgagt ggaaagggca ttaactactg acgaccccac cccgtgaaga
     5701 ccacctgaaa ccttgtatcc accaccgccg aactttcctc cagcatgaag gactgtaaag
     5761 acggtctcaa cagcaggacg gcctgttttt tcctgaatat cgactgggat accacgccca
     5821 tcatccacaa cagtaatcga atcatctggc tcaataaaaa cttgaatatg gctggcaaat
     5881 cctgccaagg cctcgtcaat tgagttatca acaatttccc agactagatg gtgaagacct
     5941 tcttttgagg ttgatccaat gtacatccct ggacgcatac gaacagcctc taagccctct
     6001 aaaacttgaa tttgactggc atcataatcc tgtgcctgca gatttttgat ttcttctgtc
     6061 atcttattcc tttttcttat atgtctaata cttgtcttaa attcacgata ctggtaaaca
     6121 agtgggtatc aagtcctgca gcttgacctg cttcgatatc aatcggccga tcaccaatga
     6181 caagaccaga gctaatctga tacttttctc ttaaataaag catggattcg ggatttggct
     6241 ttctcttaaa gcctgagcta gaagtcacca cttctgtaaa ataagctgct atagaggttt
     6301 tttctaaaat ttccaaaacc tgatcatttc gatgagagac caaaaaatga cggccacctt
     6361 gatttaaaat gtcttccaat aggtcagaaa ctccttcaaa taaaatcggg tgttcaagct
     6421 ctctggcttc attttccttg tacttttcta aaaaattctc taaattggga gcgaatgtct
     6481 caatcgcaaa aggagtagaa acctttaaag cttgatagac actgtcatgg tcttgtgtga
     6541 taccatacag tgccaatgtt tcaacaaatg cagctgttga agtttcataa ttatccagta
     6601 aagttccacc taaatcccag atgtaatcgt gatatttcat acccttcatt atagcatttt
     6661 tttatgaaaa aagcgatgat ttccctgagt tttccacata aaaaacgaga gaataactcc
     6721 cgttttactg atttttacca aagacatctc gatagagcat tcctgttcgt aaactctctg
     6781 cgtcccctcc tagttgctcc accaactcgt aggcaaaggc aagggctgtt gaaggacccc
     6841 gactggttgt caactgacca tctaccacta ctgtttcctt gacgtagtga ccatcaagga
     6901 tttgctcttg aacgccgtca taacaagtgt atcgcttatt tttcaatatc tctgcttgat
     6961 tgagggcaat tggtgccgca caaatggctg ctagtttctt cccttcttgc tcgaagcttt
     7021 gcaattcttg aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc atacctccag
     7081 gaagaacaat catatcatag tctgataaat ctccatcaaa gacatgatct gctcttactt
     7141 ggattgcatg cgaacccgtt acttgctctt caaaaccaac catatcacat gtgatattgg
     7201 ctcgacgcaa gacatctaca actgtcaagg cttcaatttc ttcaaagccc tgagctaaca
     7261 taactgctac ttttaccatg attttctcct tatttaattt gttttaatac aacctttttc
     7321 cgtttgaatg ggacttttcc gctcttttct tcagctaaat tagtctggta actcatcgat
     7381 aaaagcaaac caacaccaat caaattactg ataatagctg atcccccttg cgaaatgaaa
     7441 ggcaagggaa tccccgtcaa aggaagtagt ccagtcacag caccgatatt ctcaaagatg
     7501 tggaagagca acatcataat caaacctgtg gaaatataag tgtagaactg gttatttgat
     7561 ttaagagtaa tcttcaacat acggtaaatc aacatgagat agagggcaat aaccaggaca
     7621 gagccaataa agccaaaatc ttctgcaata accgtaaaaa tcatatctga ctctcgaact
     7681 gggataagca gattcgaagc attaaatccc tgaccaaata agccaccact cccaatggca
     7741 atctgccctt gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg attgagccaa
     7801 gccaaaatcc gattaatttg gtaggtcggc attccaatct ggtgaagaaa agctcgtccg
     7861 tccttgctaa taaagatagc taagaaacca gcaactcctg ttacagcagt cacaaatact
     7921 gggataataa ttttccaaga aacccctgat aataaaacga ttcctgagaa aatggctaca
     7981 aaaaccaaag ccgtccccaa gtcactttga agtgctaaaa gaactaggac tggaatggta
     8041 aagagaatca tccagaaaat taacaaaaag tccagcggaa ccgtgcgtct ccattcctta
     8101 tgtttctttg taaattggac aatgacacga gccaacatga ggatatagga tatcttcata
     8161 aattctgacg gttgaaatag ggtaattcca tttattgata cccagttttt ggcacccgtt
     8221 gatgcaacta agcttggatt ataaaataca atcggcaaga tcataagtcc caagcctaaa
     8281 atatatagaa agggggtcac cttccaaaga aattctgtat taaagagcat gaccacaaaa
     8341 ccaatcacaa gccccaaggc aatccaggcg acctgctgcc ctaaaatggg cagaatatta
     8401 ttgggataat catgactaac ggctatatag atagccacca caccgatgac cagtagaaaa
     8461 aatactggca agagcaaact ataatcgact ctagagtcga gagaacgttt catatgtact
     8521 aaccttatac tttcatacaa tactatttat caaagttcat taaaaaatct atcaatagcc
     8581 tcgtcaactt cggatcgaga gatggtttta acagtcgctt cttctgctag agatgctact
     8641 atttgtttgc cgtatcgttt tccgacgatt ctcctatcca aaataagagt taaggaacgt
     8701 tggtattcac gtctcatact tcttcccaaa gcctgtttta aacgaataat ggccattggc
     8761 aattgataat cataaaaggc atttttccct tcttgattca gttcttgatt aatctttttc
     8821 gtcaagggtt cttgaggatt ttggaaagga agcctcggta caacttgaat cacagaagga
     8881 tggcttgaaa aatcaactcc ctcccagaaa cttgctgcac caagcaagat ttgttgttca
     8941 cctttttcaa agcgtttctt tagctgatga acatccccat ttttatactg ggccaagtgg
     9001 ctaactgtaa gtaaatccga tactgctaga agcatgtctt tagcggtaaa gagaaccaaa
     9061 atcggttgct ggaaagcttg aatttccact agtaaagcag ctacctctct ggcatagact
     9121 tctaaggagg tttctgttac cagagggaaa tctttgacca taaccacttc ttgttcctgt
     9181 tttccccgag attcaatctt gacaaatttg gcttcaggat agcctaaaag gtctgccaaa
     9241 gaaaccctct gactaatctc aagagtagcc gatactccca agacttggca actctcagga
     9301 agtaaagagg acagaagagt acggcctgat tttgtagaag aaatctgaat tttcttttga
     9361 ctcgtttcag ttacttcaag ccagtaatca ccttcagctg taaaatagcg aaccagctca
     9421 tcaaagcctt ccacttctaa ttctgaaaaa tactgatgga gattgtccag agaatctaag
     9481 atatttttcc tagatttacc agactgaaat tgttctatca agtagagaca ctcaaagcga
     9541 atactttcta gtatccgttg ttgaaccctg ttttcttctc ctactaaagc cttatcaatt
     9601 aaatcgataa tagattgtat atcgtaggtc tcttgaagca gattttctag agctaacaaa
     9661 atcttttgga cttcatcaat aatcagtaaa cggtcactga caaattcagg attatcttca
     9721 agtctggtca cgagataggc atgattagtc actaaaagct tgcaagtctc tgccctttct
     9781 tgactacgtt tccaaaaatc ttccgtcaca aataagctct gggatgataa attcccatca
     9841 tgacgaaggt ctgctagaaa atgttggtaa cggtagagtt gcccgatttc atccaaatct
     9901 cctgtctctg tctcagtaag ccagaccaag acttgcattt taaagcgtct aaataagcga
     9961 ttttcatcat tttcctgcaa ggaatgataa aaggcatcca acttcagata attttgtggt
    10021 ccctttaagc tatgaatatc tgtatggaac acttccttga gacgtttacc ttcttcttcc
    10081 atgatttgat tttgaagaat ctttgtcgga acactaagaa caatttgtcg ctctttggat
    10141 tgagataaag cgggtaagag atagccatag gttttcccaa tccctgtcgg cgcttgaatc
    10201 agagagaaag gttcatcttt caatagcaag ccaacctctt tagcaaaact ttcttgttcc
    10261 tccctcactt caaggttcaa cagagaaata tttttagaaa agtcttgaga tagttttcgt
    10321 agctccagag aagctgccgt tttcttaaaa tacagacctt gaacttggac caagtctgga
    10381 gaactcagga tagattggtt gcgataagtt tcctcaataa ccaggtaaga ctcatatagg
    10441 agagcgtcag ccatttccag caagcgttcc aagagacctt taggaagctg ggtcatcttt
    10501 tcccgtaaaa aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc tgtgtgtgcg
    10561 tgtttaagag gaattcctaa ttctcgacac aaaatcggca agctatattt ttccagttca
    10621 gggaaaaaga cctgggccaa ttcgaccgta tcaacacgag ggtttcttag ctcatagcct
    10681 tcaaaaaata aattttccgc caagagatta gcatcaaact gaacattatg ggctacaaaa
    10741 atcccatcct ccaccaagtc aaagattttt ctggcaactt gcgaaaaatc aggtgcttgc
    10801 gccagacgtt ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa gggttcatgt
    10861 ggattgacat ccgtcgtata gtgatcgacg atttctccat cctcaatcac gacaattccc
    10921 acttggataa ttttagcctt actacctgtg ctagttgcct ctaaatccac cacaacatag
    10981 cgattaccaa tcgttttcat tataaaaaat tataccaaaa aaagggccat ttggcacctg
    11041 caaacatagg ataattttca aactcaagaa cactactttt cttagaaatc ccatcgaata
    11101 gagcattcta gcctgctgat ttttttaaaa tagtgaagaa atacgaaaaa ttgagagaag
    11161 aaatgttttc atcttccttc tctcaacgaa tcatgtattt tatttaacca tgtcaggatc
    11221 gtagtcataa aatcctgtat caaggaaacg gtttttaggg tgaagtttac gaacttcttc
    11281 atccagcaag aaggcttggt catggctaaa gttgccctct tgaatcaagc tacgcgcttg
    11341 gataaagagt tttgcaatct caacaaagtt ctgaccagga gtgtagagcc cttctagttt
    11401 ccagcgagta aagccatgct ccaccaattc tgttaatttg atcatcaaat caaggtcatt
    11461 gttggcaaag atatgggtcc catgattatc ttcaaaaatg gaatagtggc tctctggatc
    11521 acttggctca gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga tatgtgtaaa
    11581 gttatagtag ttttgcaaga gtggacgttt agaatgatgg atgacgctag caccgtaaac
    11641 caaaacttca gcaggaattt ccaaaatctc tggcattttg aaaagttcag ctgatggaat
    11701 ttcacgcgcc aaaacagcct cagatgcgcc agccttttgt ccccagaagt taatctgacg
    11761 actgctagtt accatggttg aagcatcgta gatggtctta aatgaataac catcgcggtt
    11821 aactacgtaa aagacgcctg catccccaat cgtaatatag tctgtcttga tttcttccaa
    11881 gaagtctaag aaaggcttga tacggtccat catatcttgg tgcatgagag cattgaccgc
    11941 aacgatcaat tccttaccag catcatgaac caacttagcg atttcacgta attggtcata
    12001 actaaaggtc gttggcagac gaaggccaaa atctttctca ccgacataga tacggtctac
    12061 gccagcttcg agtagttgtt caacttgttc aatactttca gcagttgctg taatgacaat
    12121 cttttccata agaaaaatta taccacattt ctccaaaatc agccatgctt tgaaaatgaa
    12181 aagggacttt attctttttg taacctttct gtaataattc tctgccgaaa aaatgataaa
    12241 ataggtatgt actgttaagg agagaatcat gcccgtaaga aaattacaat cctatgaggt
    12301 agactatcaa gaagaattaa accagcagct tcctcattac caagcttata cacctgaagc
    12361 gcaatctgat gccaatctca aggaaatcct attttttatt aatatcgctg ttttttgtat
    12421 ctgtattgct atctttagtt ttatcttttt agcattaaaa ttatcaactg ctcttgcctt
    12481 tgccgcagca atcggattca gcttacttgt tttaaaagtt caacggtcta ttatcaaacg
    12541 aaaacgtaga agataaatcc aaaatatcgc cccaattgga gcgatatttt tattttttct
    12601 ttttagatgg tgtctgaatg gcaatcttca cttcaaagat tgttcccttg ggtttattat
    12661 ctttgacagt aacagttcct tttagagcat ctacaatttg cttggctagg gataatccta
    12721 aaccaaaacc acctttttgc cgggttctag ctttgtctac tcgataaaaa cggtcaaaaa
    12781 ttttcttttt atcttctgtc gaaataccga ttccattatc agaaacaagt aaataaagat
    12841 tgcgatcggt cgccgagata agaaaatcaa tttcaccatc ctcctcagta tacttgacgg
    12901 cattatcgaa aagaatggtc atcagttgtt tcagaagaag ctgatctgtg acaattgttc
    12961 gatggatacg attttcaaaa cggaagacac gattattttc cgaagcaatc atctcatagt
    13021 ttgtgaaagt tgtattaaaa aagctagttg gaacttctgc aagctccggc ttaatcccat
    13081 catctctccg agctaagttc agcaagcttg tcgttaaaaa acgcatattt cggacttctt
    13141 ccaaactcga tgcaatgctt tcgctcacat ccataatggt agcttctggc ttacgaaaaa
    13201 gggtctctaa gcgattttgc aaaactgcga gtggagttcg taactcatga ctggcatttt
    13261 ccacaaaaga ctgttgcttc tgcatactct caagcagggg cctaacactg accctagcta
    13321 gatagagact ggcaagtaaa gacaaaatcc agaaactagc catcacgacc acaatcaatt
    13381 gctcatgctt ttgactagcc tgctccaact gactggtatt aatcaagaca gcagcatact
    13441 tgatattggt tgaaaccgaa ctaatattgg tctccatcaa aatcacacga tagatttctt
    13501 cctgcccata gctattaaaa acctgaatct ggtagatatg tcctagttct ttcttttcta
    13561 acttaatctt atccaagccc aaaaatcgat ttccagaaag aagttgagta aagtctttat
    13621 caaagagaat gacttccgta ttggaactga cattaggttt tatttctact ttactagcgt
    13681 ccgccctagc attttctaaa tctttaatct cttctgttgc cctatttata gccagctgaa
    13741 taactgcttg aggattttca ctcagtccat gaagcttatc gtccaccgaa gtataaagac
    13801 tcgaatgcat gacttgtaaa ataatcagag tcattgtaga aaaaatcagg gtgaagacac
    13861 cgaagttgcg gataaaataa ctaaagtcat ccgcatacca tgttttttta agtttactga
    13921 acatctttta aaagataccc aacactacgc aaggtttgca aattctctgc aaaagtggtt
    13981 ccctttaatt tcttacggac ttttgaaaca tagacttcga caaccgaaat cgttgtatca
    14041 ctatcaaatc cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac attttgattt
    14101 tgaaggaaat aaactagtaa atcgaactct ttccccagca attcgacagg agtatcttca
    14161 actttaacgg tattggttga taaattaacc acgatattcc cataagtcaa ggtgttttca
    14221 ttaaacttcc ctgaacgttt gagaagggcc tgaattcgca ttttaagttc ttctaggtag
    14281 aaaggtttgg tcagataatc atccgctccc agttcaaatc catgtccctt gtcatccaaa
    14341 ctttccttgg cagtcataat cagaactggt gtcgtaattc ccttttcacg caattctttt
    14401 aagacttgga aaccattttt ttctggcaac atcaaatcca gcaaaatcaa gtcatagaca
    14461 ccactctcag cttcgtagag accttcttct ccatcaaata cctgcataac atccgcaaaa
    14521 tcgtctaaaa agtcaaatac tgaatttgac agacctaggt catcctcaac caataagatt
    14581 tttatcatga gaaactcctc cttattaaaa ctattatacc aaatttgcct taaaaaaaac
    14641 tcaactctct gcattttaca tgagatagct gagttttctt
//
LOCUS       DAYYQN010000049        14157 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYYQN010000049 DAYYQN010000000
VERSION     DAYYQN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.049"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(25..924)
                     /locus_tag="ACHBRQ_001549"
     CDS             complement(25..924)
                     /locus_tag="ACHBRQ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9593186.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(996..1556)
                     /gene="rnmV"
                     /locus_tag="ACHBRQ_001550"
     CDS             complement(996..1556)
                     /gene="rnmV"
                     /locus_tag="ACHBRQ_001550"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9593187.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1556..2329)
                     /locus_tag="ACHBRQ_001551"
     CDS             complement(1556..2329)
                     /locus_tag="ACHBRQ_001551"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9593188.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMIISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2634..3386)
                     /locus_tag="ACHBRQ_001552"
     CDS             complement(2634..3386)
                     /locus_tag="ACHBRQ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HFM9593189.1"
                     /translation="MCPVIILFYYKVEVGGDGTATITFPDGSVVTILGKDTVQQSAKG
                     ESVTQEATPEYKLETTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASK
                     QLATEKESAKNAIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTM
                     EDVKEAETIGVQAIAMVTVPKRLVAPNAAPKTTSAPQATAGTMQNVTYQSPAGKQLPN
                     TGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            complement(3562..3696)
                     /gene="rpmH"
                     /locus_tag="ACHBRQ_001553"
     CDS             complement(3562..3696)
                     /gene="rpmH"
                     /locus_tag="ACHBRQ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9593190.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(3841..5055)
                     /locus_tag="ACHBRQ_001554"
     CDS             complement(3841..5055)
                     /locus_tag="ACHBRQ_001554"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9593191.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5211..5504)
                     /locus_tag="ACHBRQ_001555"
     CDS             complement(5211..5504)
                     /locus_tag="ACHBRQ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593192.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVVKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            5667..6119
                     /locus_tag="ACHBRQ_001556"
     CDS             5667..6119
                     /locus_tag="ACHBRQ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9593193.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6154..7542)
                     /locus_tag="ACHBRQ_001557"
     CDS             complement(6154..7542)
                     /locus_tag="ACHBRQ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9593194.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            7608..8570
                     /locus_tag="ACHBRQ_001558"
     CDS             7608..8570
                     /locus_tag="ACHBRQ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9593195.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(8776..9786)
                     /gene="ccpA"
                     /locus_tag="ACHBRQ_001559"
     CDS             complement(8776..9786)
                     /gene="ccpA"
                     /locus_tag="ACHBRQ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9593196.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(10211..10810)
                     /locus_tag="ACHBRQ_001560"
     CDS             complement(10211..10810)
                     /locus_tag="ACHBRQ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9593197.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(10812..11909)
                     /locus_tag="ACHBRQ_001561"
     CDS             complement(10812..11909)
                     /locus_tag="ACHBRQ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9593198.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(11909..12646)
                     /locus_tag="ACHBRQ_001562"
     CDS             complement(11909..12646)
                     /locus_tag="ACHBRQ_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593199.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(12648..13532)
                     /locus_tag="ACHBRQ_001563"
     CDS             complement(12648..13532)
                     /locus_tag="ACHBRQ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9593200.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(13519..13713)
                     /locus_tag="ACHBRQ_001564"
     CDS             complement(13519..13713)
                     /locus_tag="ACHBRQ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593201.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(13710..13916)
                     /locus_tag="ACHBRQ_001565"
     CDS             complement(13710..13916)
                     /locus_tag="ACHBRQ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593202.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         4193 a         3269 c         2505 g         4190 t
ORIGIN      
        1 atttttgtca ctgattcact atgtttataa atattttttg acatcctctt gccattcctc
       61 tgtcaatttc tttactagaa atccatctcc aattagcttc gcaaatgttt tagaagactg
      121 ataaaatgat tcagctaaac taaaatctct ttcttctctt aatgcttgct tccacaaaaa
      181 cataaataca agaggtttct tttgataatc ttgagtttta tccataattt cttgtaaaca
      241 cgatagataa tcattaaaac aatcagttac tcctaccttt tcaagacaac acattcctga
      301 aataagagta tctctaatta taaagaagtt ttctaattta gttacctttt tctgttccaa
      361 aagaaagagc attagctttt cgatttgatt tataaatttg gcaagatctt ttctaatgag
      421 catttgataa aaaaacaaac gaatcaacaa catatcattt gttgaataaa cttctttttc
      481 tatgatctga gacatatgtt cctcaagtat taactctgca tattcaggcc ttctagttct
      541 agaagtatcc aaacacgcct gcaatacttc acatgctaat tgttcttctt taggaagatt
      601 atcaaagtat gttttataaa tctcttccaa acacgcttcc ttcttatcgt actcctcttc
      661 tttaccatag attggttcac gtaaaatctg gtatttcaat tctaaataag caggaggaag
      721 agctgaaaaa tccggcataa ggctataaac tggaactcct agccggcgag caatatagtc
      781 taacttgggc aaactcggtt gcgaagctcc actttcaatt cgcgacagtt gacgaactgt
      841 cagttcttgc tcatctccac aaaaatctgg acgactaatt cctttttcta tccgaatctc
      901 tttaattttt tgaccaatta acatcagacg cctcctcttc tacctgcttc gaaaaatact
      961 gtgaaactta aaaaattgta acggctacat ctttcctact cataagattt catagcttct
     1021 tccacttctg ccaaagtaac cccaaacaac tctaggcgtt tgaggagttg cttgccgttg
     1081 gaatagccga ttcggagagt ctctccgaga tattctctac gcttacggct gtctgcccct
     1141 gctagaaaac caaggcgaat caaatcgcta cgactaatgt caaactgact ctcatgttca
     1201 aattgttctg tcacctgagc tagagccgtt ttcaggtctt catagctggc atgctcaatt
     1261 cccagagaac gccccttggt cttggacttg ggaacagctt catctcgctt gagaaaggca
     1321 tgctgaactg ttggaatgac cgtcatgatc atgcgccgaa tccgttcccc attaaaatct
     1381 gggtctgtaa agacaatgac tccatgacgt tggtgtagac gctgaattcg ctctatatcc
     1441 tgatcattga tggcagaacc tcgtgtctca taggtctcca catcgaaata acgcttgaga
     1501 ttgaccgtat catagcgacc ttcaaccacg ataacttgag aaattttctc tttcattact
     1561 tgctgtccaa tccaaaaatt cgttctgcat ttgcagtcgt tgctaccgcc agctcttctg
     1621 tcgtcatacc acgcaagtca gcgataaagt cgaccacata gcgagtatag gctgttttat
     1681 tttcacgacc acgcttgggt acaggtgcta agtaaggcgc atctgtttcc accaacatct
     1741 tgtccaaagg taactcttta gctgcttctt ggaggtcagt tgccttcttg aaagtcacca
     1801 ctcctgagaa ggaaatgatc ataccaagat ccacaaactt ctctgcccac tcaagcgtcc
     1861 ctgaaaatga atgcatgata ccaccacgag gaccaacgcc ctcactcttg ataatctcat
     1921 aggtatcttc cagcgcatca cgggtatgga caacaaaagg caaatccaag tccttagata
     1981 gctgaatctg acggcgaaaa acctgctcct gcacctcttt gggcgctgtc atccaatggt
     2041 agtctaagcc aatttcacct aaagccacaa ccttgggatg ttttaactta tccaacaagt
     2101 aagcctcaac ttcctctgta taagtaccag cttctgtagg atgccaacca atagtcgcat
     2161 agagctgctc atactcatct accaactcca aggcacgctc aatcgtcggt ttatcaaaac
     2221 caacaatatt catctgtatc acacccatct cagcagccaa ggcaatttct tctgcctcac
     2281 gacctgcaaa ttcttctaca ttcaagtgtg tatgtgtatc aaaaatcatc tcttctaacc
     2341 tcgttttcta tcttctatta taccaaaaaa gaggaggggc acctaatttt tcggtttccc
     2401 ctcctctctt caatagagag ctattctgct atcttttcta tccgatattg cctatctcct
     2461 attccacagt tagagacaga agagattggc tacattgata gaatattcct attcagctaa
     2521 agtatgtgat tatcttgggt tctcactttt ctcacccatc actaggattg atgacttctc
     2581 aaaatccccc ttataatcta actttaagta gagaatagaa ttttctagct gttctatttt
     2641 ctacgtctag tctttcttcc tagcaaagca aaaccacttg ttgccaccac tagaccaaga
     2701 ctagcaagtg ctgcacttga tgctgaacct gtgttaggta attgtttgcc agcaggtgac
     2761 tggtaggtaa cattttgcat tgttcctgca gttgcttgcg gtgcacttgt tgtcttagga
     2821 gcagcattag gagccactag tctcttagga actgtaacca tggcaatggc ttgcactcca
     2881 atcgtttctg cttccttcac atcttccata gttttcgcat tttcaatctc tttgagagct
     2941 gcttgttttt ctgcttccac tcttgctaaa agttctgctt tttctttatc agaaagcggt
     3001 gcgcctttga tttcatcctg cttgttcttg gctgcttttt caatggcatt tttagctgat
     3061 tctttttcag tagctaattg cttagaagct tgtgattgag ctgagttttg tgaacctgtg
     3121 tgagctgatc cacccgcctg gctaccaccg ccttcattcg cattagcatc tgagcttcca
     3181 gtattgcctc ccttatctcc acctggtgta gtttctagct tatactctgg tgtagcttct
     3241 tgagttacag attcaccttt cgcagattgt tgaactgtat cttttcctag aatcgtcact
     3301 actgaaccat ctgggaatgt gattgttgct gtaccatctc cacctacttc aactttatag
     3361 taaaataaaa taataacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt
     3421 tttagaaaca gaggtgtact attctagttt caatctactg taacatcgct tccgaatcca
     3481 tttgcatcta ctgaactttt ttacaaaaat aaaaatcagt agactcgagt tcctactgat
     3541 ttttgataga attcgtttgg attatgcagc caaaactttg cgtcctttac gacgacgagc
     3601 tgccaatacg cgacgaccat ttttagttga catacggtta cggaatccgt gtttgcgcgc
     3661 acgacgaagt ttacttggtt gataagtacg tttcacgatg aatacctcct catagatttt
     3721 gtattcgttt agccggctat aaagtgatca gttactaaac atactttact attctatctg
     3781 attctttcac ttttgtcaat agctctaggc aaatgtttcc agctttttcg aatgcaagcc
     3841 ctatctacga tactgtttca agaaacgagt catcttttct tggatttggg ctaactcatc
     3901 aacacgagga aggtaaacga tacggaagtg gtctggctcc tgccagttaa agcctcgacc
     3961 atgaaccaag agaacctttt cctgcttcaa gaaatcaagg acaaactgct catcatcatc
     4021 gatacggtac atattgcggt cgatttttgg gaagatatag agccccgcct tgggtttaac
     4081 ggcagacaaa cctggaatat cttgaatggc attatagatg aaatttcttt gctcgtagat
     4141 tcgtccacca ggaagaagca attcatcgac tgattggtgc ccccccaagg aagtttgtac
     4201 gacttgttgg gccaaaacgt tagagcaaag gcgcatattg gacagcatat tgagcccttc
     4261 gatatagccc ttaacatgag tcttagggcc agacaagacc atccaaccca cacgaaaacc
     4321 tgctatgcgg tgggattttg acagaccatt catgctgaca cagaagacat ctggtgccaa
     4381 gctcgccaca ggcgtatgca catgtccgtc cattaccatg cggtcataga tttcatccgc
     4441 aaagataatc aaatcgtttt gacgggcaat ctcaataatc tccagcaaga gttccttagg
     4501 ataaagggct ccagttgggt tatttggatt gataaggacg attgccttgg tattggaagt
     4561 gatttttgac ttgatatcgt caatatctgg gtaccattca acagcttcat cacagatata
     4621 gtgaacggca tttcctccag ctaggctgac agccgccgtc catagaggat agtctggcat
     4681 tggcactaag acctcatccc cattgtccaa aagcccctgc atggacatga caatcaactc
     4741 actgacacca tttccaaggt aaatatcatc aatatctacg tttgggaatt tcttcagctg
     4801 gcaatactgc atgatggcct tacgggctga gaaaatccct ttggagtcag aatagccttc
     4861 actatcacgc gcattcataa tcaagtcatg aatgacctcg tctggcgctg taaagccaaa
     4921 ttctgctgga tttcctgtat tcagacgtaa aatcttttct ccattcgctc gcatccgcat
     4981 ggcttcttcc aatacaggac cacggatatc ataagcaaca tgctctaact tactagactt
     5041 gttatattct ttcatcttgt ttcctcaatt catcaggata gtaacattat accactagaa
     5101 agagaatgcg acaagtttac tgaaatgtgt tactagtttc gtatagaaga aaaaccttgc
     5161 taaaggcgag gttaagaacg tagagatagc aggtattttg caaagacaca tcacatacca
     5221 aaagataaac ctagaaaaag caagcctaga aaggagtcat aaagaagatt aaacatgagg
     5281 aaaagccccc atatcatcaa gtaatcccaa cgcccacttc gttttacaac tagaaataag
     5341 attagatggt agagtacatg aaagactaaa aatccaataa aacttggata aagaagcggt
     5401 accagactct ctaattgaca gatcatgatc acttctacca agactgaaac taggataatt
     5461 ccataataaa ggaaatttga tttttgaaaa gagtttttca tcattctccc ttccttcact
     5521 tctttcctgc tatcctttct tttattttat catatattta tatagagttt aaaccctaat
     5581 attcttttga aaaatacttt tttttgcttt acttctatag tgaaaaggtt tacaattata
     5641 gtaagaaaat ttcaggaggt ttcattatgg cacaacgtta ccaaaatatc atggtcgcaa
     5701 tcgatggttc taaggaagcg gacttggctt ttgtcaaggg agttcattct gctctacgaa
     5761 acgacgctaa actcaccatc gcacatgtca ttgacacacg cgctctccaa agcgtatcca
     5821 cctttgatgc tgaagtttac gaagaactcc aagtcgacgc tgaaagtctg atgaaagagt
     5881 acgaaaaacg tgctaaagat gctggggtag cagatgttca tatcgtcatt gaaatgggaa
     5941 atccaaagac cctgctagca cgtactattc cagatgccga ggaagtggac ctcatcctcg
     6001 ttggcgcaac tggtctcaac gcctttgaac gcctcttagt cggctcttca tctgaataca
     6061 tactccgcca tgctaaggtc gatttgctgg ttgtgagaga acaagaaaaa accttataat
     6121 cacaaagaaa aggagcccct agctcctttt tgtttacgat ttatttctct ctttatggcg
     6181 ttcgtaagcc ttgagctggc gctgcagttc ctttttaata gcaggttctg gagcatattt
     6241 ttcttcccaa ttatctggtt ttaagatttt atgggtcact ggatcaaaat gagccttgcc
     6301 atctggaaaa attttcccca tattggcctg atggacaata tcaaaaatac gttctgggtc
     6361 cacccccatc aagacaaaac tgccgtaggt gaagtaaagc gtgtcaatca aggcatccac
     6421 ttgccctatc aaatcttgct gagcaggtgt cttcttggct actttacctg ctgccttatc
     6481 aagggcctga tgaagttgcg atacagcttg accaaaatct tcttcagaag gactggctga
     6541 tcgaacaaac tccaccaatt cttctatttt aaaaccagcc ctatgggttg caccctctaa
     6601 atcccaagct cgaggttctt cttgggttcg ttcatccatc atgtggtgga aggtcttgac
     6661 cttattgaaa tgatagtcac ggctgacaaa aactttttct gaagacaaaa caccaaaatg
     6721 ttccaaggcc ttgtggatcc catcttgttg attgctagtg gtaatgtgct tggcagcttc
     6781 cttgacactg ctactaccat ttcccatagc gaccgacata ccgacgccag ccagcatttc
     6841 aaggtcatta tctgagtcac caaaagccat gacttggttg aggtcaaagc catattcttt
     6901 cccaactcgg cgaatgcctt ctaatttaga atttccctga ttgatgacat ccgatgcaaa
     6961 aggattgcta cgtgtcaatt tcaagtcttc aaaatcagct gccgccttct cagattcttc
     7021 tggtgtcatc agcatcaaaa cttggtagat aggctgattc atcaggtgaa gcaggtcctc
     7081 ttccttttgg ggaacaacct tgctgaccat gcgattaaaa gactgactca ccgtccgagt
     7141 taaaacagag ggaacgaagc gactaattcg ttgggaaaaa gaacccagac caaaggacat
     7201 gattttagaa cccaacatgg catccttggt ccctagagca atctccgtgc cctctttttt
     7261 agcatagcta attagatggc gcaaatgtaa cttggaaata gggctcgtga acaagactct
     7321 gtctttacta aagatatact ggccattata ggttaccgca aaatccagat ccaaatcgtc
     7381 catcaattcc ttaacaaaaa aaggtcctcg ccctgtcgct acgccaacta gtaccccttg
     7441 ttctttgaca atcttaatcg catccttagt ggatttcaaa acactcttgc gattgttgac
     7501 caaggttcca tcgatatcaa aaaaaacagc tttgacttcc atcctatccc aatctcccct
     7561 tttgtgatac aatgattata ccacatttca gaaagagtga gtaaatcatg cctaagaaaa
     7621 tccttgtttt acatacgggt ggaactattt ccatgcaggc cgatgcttct ggcgctgttg
     7681 tgacgagttc agataatccc atgaaccatg tgtccaaccc acttgaagga atccaagtcc
     7741 acgccttgga cttttttaac cttccaagtc cccatatcaa acccaaacat atgctggtcc
     7801 tctaccagaa aattaaagag gaagcagata actacgatgg agtggtgatc acacacggaa
     7861 ccgatacttt agaggaaaca gcctatttcc ttgataccat ggaagttccc catatgccta
     7921 tcgttctaac aggagccatg cgtagctcca atgagctcgg tagtgatggt gtttataatt
     7981 acctaagtgc tttacgagtg gccagcgatg acagggctgc tgacaaagga gttttggtcg
     8041 ttatgaacga tgaaatccac gctgccaagt atgtcaccaa aacacatacg actaatgtca
     8101 gcaccttcca gactccaaca catggccccc tcggtctcat catgaaacag gaaatcctct
     8161 acttcaaaac agctgaacct cgtgttcgct ttgaccttga tcacatacaa ggtttagtcc
     8221 ctatcatctc ggcttatgct ggtatgacag atgagctgat tgatatgctg gatttagaac
     8281 acttggacgg tttgattatc caagccttcg gagctggtaa tattcccaaa gaaacggctc
     8341 aaaaattaga aagccttctg caaaaaggaa tcccagtcgc tctggtatca cgatgcttta
     8401 acggtattgc cgagcctgtt tatgcctacc agggtggggg cgtacagttg caaaaagcag
     8461 gcgttttctt tgttaaagaa ctcaatgccc aaaaagctcg cttgaaactc ctcatcgccc
     8521 tcaatgccgg actaacagga caggctttga aagactatat ggaaggctaa tactcttcga
     8581 aaatctctgc aaaccacgtc acgtcgcctt accgtatgta tggttactga cttcgtcagt
     8641 ttcatccaca acctcaaaac agtgtgttgc gcagccttta accagccgcc tagtccgctc
     8701 tatggtattc attaagtcaa catctcttgt ttaagagcac aaaatcagga aatcttctcg
     8761 attccctgat tttttctatt tacgttttcg tgttgagcta cgttctgtca aaccatgagg
     8821 taagagaact tcacgttctt ccaactcttc cttatgcata atcttggtca acatacgcat
     8881 actaatggca ccaaggtcat aaagaggttg ggcaatcgtt gtcaagtttg gacgggtaaa
     8941 gcgtgagatt tgtgaatcat cactagtaat aatttcaaaa tcttctggca cagaaacacc
     9001 cttatcagcc aaaccgttca agactcctgc tgccaactca tcacctgtca caactgctgc
     9061 agttgcattt gatgaaatca aacgctctgc taaggcgtaa ccatcatcat agctatattt
     9121 agattcaaat accaaaccct cactataagt gattcctgct tttttcaagg tttccttgta
     9181 gccaactaaa cgaaccttac cattgatgtc atccactagc ggaccgctaa cgaaagcaat
     9241 acgctcattt tctttagcaa ggtaactcac tgcatcaatt gttgcttgct tatagtcaat
     9301 attgacactt ggcaactggt gctcaacatc gacagttcct gcgagaacaa tcggagtacg
     9361 tgaacgcgaa aattctgagc gaattttatc tgtcaagtga taccccatat agataatgcc
     9421 atctacctgc tttgaaaaga gggtattgac aacagaaact tctttctcgt tatcttcatc
     9481 gctattagct aggacaatat tgtacttgta catttctgca atatcatcaa tccccttagc
     9541 caaactcgaa aaataaccat tggtaatatt tggaatcacg acaccgacag tggttgtctt
     9601 tttacttgca agaccacgcg caactgcatt tggacgataa tccaaacgat caattacctc
     9661 tagcactttt ttacgggtat tctcttttac atttttattg ccattgacca cacggctgac
     9721 cgtcgccatg gaaacacctg cttcacgagc gacatcataa atggttactg tatcatctgc
     9781 attcattcct tttcctgtcc tttctatctc acacattctt ttacaagtag aggtactgat
     9841 tgaagctcta tatctactta caaaagtgaa gatgtgaaaa tttcgttttc atatttctac
     9901 ttattccatt ctatcactaa ttgtaaacac tttcaagtgt tttttgaaga ttgattgaaa
     9961 aaatttcata gaaaacctag gtttagctcc ttgctaccac cttagactaa acaaaaagga
    10021 ggaaactaag ccctcctaaa gttatagtaa aatgaaataa gaacaggata aatcgatcag
    10081 gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa
    10141 tctactatag gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa
    10201 tattatatca tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac
    10261 cagcatccag ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt
    10321 aaagctgatc tgcgatttct tggttggata agccccgggc gattcccttt aagacagcaa
    10381 tctcttgttc tgtcaacgga ttgggacggg tcatcaccat ttccatcaat tcaggcgaat
    10441 actccttgcg tccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcgtt
    10501 cctttaatac ataagcatcc actccagcct tgaccgcacg ttcaaaatac ccagcacgct
    10561 tgaaggtcgt caccacaacc acctttgttt caagcttttc tgatcgtatc cactccaaga
    10621 cttcaagacc tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct
    10681 tttctaatag ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg
    10741 gttgaagcgt gagcaattga cacatggcat ctcgcaacat actttgatct tctgcgacta
    10801 atactttcat cttagtttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt
    10861 ttctgactga ttacacttac ttctcctgaa aatggaaaaa cacgatctcg gactgtatgg
    10921 agctcatctc cttttagaaa agcaaagccg cagccatcat cacttactgt taaaatgagt
    10981 tccttctctg tccgttctaa ttttaagtaa gctttagacg ctttggcatg tttgatgata
    11041 ttggtcacca actcaagcaa aatcatagaa gccattgact ccaattcttg agtaaggcta
    11101 gcagtatcta gttggttatc cgtttccacc tcaattccag caatttctaa catctttttc
    11161 acagtctcta gttcggatgt caaagttcta gacttaagat tttccacgat agttcgaact
    11221 tcattcatgg aatccttgct gatctggtga atttctttta attccttttc cacctgtgga
    11281 taagcctcca tctgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag
    11341 gtatgcccca gactatcatg caaatcttga ccgatacgat tgcgttcatt ttcagcaagc
    11401 aatagattta tctgggcatt ttgcttagcc tgtgcttctt tcaaatcctc cacaattcga
    11461 atccgaacca agccaaaagt cattaaatcg acaaaagcaa gaattacaag tagatagaat
    11521 agaaactcaa cttcgattct ctgaaaaatc aacagttgac ccacaacaag gacttgagca
    11581 agaagaaaag tccagacatg taaagacttt aaaccaccta cgctgaaatg atagcttaag
    11641 agattggata ggaaaaagaa aaaccagata taattaacag ccacaaaggc agtattccca
    11701 actacataag tcagcaggat gccccaatat agccaagata gacgctggct cttagttgtt
    11761 aaaacaccca aatacgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc
    11821 cacccagtca ctacagttag gatggggaaa accataaaaa ttaaactgat ccaaaacata
    11881 taatgtatgc ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa
    11941 tggtcaaacc aaacaaaact actgaaaaaa caagtaaata aactgtggct gatagattga
    12001 tgccaccctc atttaagaag gtcttgagca actccatcaa ctgataggtc gggagacact
    12061 tacctactac ttgcatccag tctggaaata aagagatagg catccagagt ccacctaaaa
    12121 cagccaaccc tagataaaga agattgccca cgacagacat caactgacta gttggtaaga
    12181 gagtcaaggt caaaccaagc gctacgaagg caatacttcc tactatcagc aaaagtgcag
    12241 ccccaatcca atttccaaga gacatgtcca cacctcttac aaaatgccca actgagaaaa
    12301 ccaccaagat tgaaaccaaa taatcaacca gcatacttgt tatctttgat agataatatt
    12361 ctaccatatt tacagggcta tgacgcaatg ttttctgcca gttgttgatc ttgtcggtat
    12421 gtaaaacaac tgggaatgag aagatagctg ttgacatcat ggaaaatgca gtcatggaga
    12481 taagataatc acgcataaaa ttcgcgagtt cacctggtgt gtcctgatag ataccagaaa
    12541 aaaataaata gaaagccgtc ggcatcccta ccgacaatag ataatagatt aattgtcgtt
    12601 tggtcaataa aaattctatc ttactaagtg ctatccatcg tttcatctta gttatctccc
    12661 ttctgcgtct cttcaaagat tgtattcaac aaactacgat tattaacttc gatttcttgc
    12721 atcctacatc ctgcttgggc taatagttcc cagaaagcat ctgcttctcg agtgactact
    12781 tgtaaagaat cttgctttag ggtccagttt tcaaccaaat ttgactgctc tacgacttcc
    12841 ttgtaagcta taggaaggat aaagtgcttt tctatttcct cgctacgcat agctagaggc
    12901 gtcgtatctc gaatcaactc tcccttattt aagagcaaaa tgcggtctgc agtgtgttcc
    12961 acttcctcaa tataatggga cgaatagaga atggtgactc cttgcgcttt taactcctgg
    13021 acaatttccc aaaaacgttg acgagttgag gtatccatgg acgcagtagg ctcatctaaa
    13081 aaaacaatct ttggtcgccc aattaaggtc aagatgaaag aaaagagacg cttttgaccg
    13141 cctgataatt tttctgccaa ctgttctttt tgttgcttga caaactgcaa tagttgatca
    13201 atttcctgat tgctcaaagg atttggatag atacgttgaa agaaagcaat caactcttta
    13261 acttttaatt tctgaacgat tatattttct tgagggaggt aagccctagt atagtctaac
    13321 ttagaactcg tcactggtaa accttgaata gatacttgac cgcttgtgac cagtttatct
    13381 ccaagcaaac aatctaagag tgttgtcttt ccggcaccat ttggaccaat taaggcaaca
    13441 cattcaccat cgttgatttc aaaggagatg ttccgcaaaa tttccttgtc ttttattttc
    13501 ttactcaatt tttcaacttt aaccacagtc atgagactct cctttcaacc actccattcc
    13561 catagggaaa acgacgaaaa tcataaatcc aaaccccaaa gcaccacaaa tgaattggcg
    13621 aagcaaggct tgatcaaacc aatcagtaaa catttccact aaccatacca agagtgacag
    13681 gccgataaag aaatagatga tccctctctt cattcctcaa actccttttt cacatctctg
    13741 actaatttca aaccttctct aacaagccaa gacatgattc caaagccagc aaagaccttc
    13801 caaggaaaat ggtagaaatc ctcatccaat cccgaaaaca tgagataggt cataactcct
    13861 gctactacta aactcattgc aataatcatt ttatttctca tctcttcttc ccccatttca
    13921 tactacaatt atagtctttt aaaatcagag gaggcagaag cttctgtcac tagaaaatat
    13981 gacaaatgtc ataaaaattc tgttcaaaac aagcaagata cactatacaa taaaacacaa
    14041 tcagaaaaat ctaaggcaac ttcctcaaaa gagatatcaa acccaattca caccataatg
    14101 taaactaata cttatttaaa atcaaaaaga gtagaaattt ttatcagaca aacacat
//
LOCUS       DAYYQN010000050        13364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYYQN010000050 DAYYQN010000000
VERSION     DAYYQN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.050"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            137..2317
                     /locus_tag="ACHBRQ_001566"
     CDS             137..2317
                     /locus_tag="ACHBRQ_001566"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9593203.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2369..3184
                     /locus_tag="ACHBRQ_001567"
     CDS             2369..3184
                     /locus_tag="ACHBRQ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9593204.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            3324..4667
                     /locus_tag="ACHBRQ_001568"
     CDS             3324..4667
                     /locus_tag="ACHBRQ_001568"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9593205.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            4882..6072
                     /gene="metK"
                     /locus_tag="ACHBRQ_001569"
     CDS             4882..6072
                     /gene="metK"
                     /locus_tag="ACHBRQ_001569"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9593206.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6625..7560
                     /locus_tag="ACHBRQ_001570"
     CDS             6625..7560
                     /locus_tag="ACHBRQ_001570"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9593207.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7594..8631
                     /gene="holA"
                     /locus_tag="ACHBRQ_001571"
     CDS             7594..8631
                     /gene="holA"
                     /locus_tag="ACHBRQ_001571"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9593208.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8772..9377
                     /gene="sodA"
                     /locus_tag="ACHBRQ_001572"
     CDS             8772..9377
                     /gene="sodA"
                     /locus_tag="ACHBRQ_001572"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HFM9593209.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9535..10065
                     /locus_tag="ACHBRQ_001573"
     CDS             9535..10065
                     /locus_tag="ACHBRQ_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9593210.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10086..10988
                     /gene="rlmN"
                     /locus_tag="ACHBRQ_001574"
     CDS             10086..10988
                     /gene="rlmN"
                     /locus_tag="ACHBRQ_001574"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9593211.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            10988..11470
                     /locus_tag="ACHBRQ_001575"
     CDS             10988..11470
                     /locus_tag="ACHBRQ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9593212.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            11472..13013
                     /locus_tag="ACHBRQ_001576"
     CDS             11472..13013
                     /locus_tag="ACHBRQ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9593213.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(13068..>13364)
                     /locus_tag="ACHBRQ_001577"
     CDS             complement(13068..>13364)
                     /locus_tag="ACHBRQ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9593214.1"
                     /translation="FHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKNKEKIVNAL
                     QLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTNFVLSRA"
BASE COUNT         4014 a         2467 c         2936 g         3947 t
ORIGIN      
        1 ctagaaattt tttagaaaaa agtgtaattt ttttataaaa gcctttacaa aaaaaattaa
       61 agtggtataa ttaaatcata gaaagtgcaa acgttttcgt aaaacgtttg cctaaataaa
      121 aataaaggag atttgaatga tgaaagatac attcaaaaat gtcttgtctt tcgaattttg
      181 gcaaaaattc ggtaaggctt tgatggtagt tatcgcggtt atgccggctg ctggtttgat
      241 gatttcaatc ggtaagtcta tcgtgatgat taacccaacc tttgcaccac ttgtcatcac
      301 aggtggaatt cttgagcaaa tcggttgggg ggttatcggt aaccttcaca ttttgtttgc
      361 cctagccatt ggaggaagct gggctaaaga acgtgctggt ggtgctttcg ccgctggtct
      421 tgctttcatc ttgattaacc gtatcactgg tacaatcttt ggtgtatcag gcgatatgtt
      481 gaaaaatcca gatgctatgg taactacttt ctttggtggt tcaatcaaag ttgctgatta
      541 ctttatcagt gttcttgaag ctccagcctt gaacatgggg gtattcgtag ggattatctc
      601 aggttttgta ggggcaactg cttacaacaa atactacaac ttccgtaaac ttcctgatgc
      661 actttcattc ttcaacggga aacgtttcgt accatttgta gttattcttc gttcagcaat
      721 cgctgcaatt ctacttgctg ctttctggcc agtagttcaa acaggtatca ataacttcgg
      781 tatctggatt gccaactcac aagaaactgc tccaattctt gcaccattct tgtatggtac
      841 tttggaacgt ttgctcttgc catttggtct tcaccacatg ttgactatcc caatgaacta
      901 cacagctctt ggtggtactt atgacatttt aactggtgca gctaaaggta ctcaagtatt
      961 cggtcaagac ccactatggc ttgcatgggt aacagacctt gtaaacctta aaggtactga
     1021 tgctagtcaa tatcaacact tgttagatac agtacatcca gctcgtttca aagttggaca
     1081 aatgatcggt tcattcggta tcttgatggg tgtgattgtt gctatctacc gtaatgttga
     1141 tgctgacaag aaacataaat acaaaggtat gatgattgca acagctcttg caacattctt
     1201 gacaggggtt actgaaccaa tcgaatacat gttcatgttc atcgcaacac ctatgtatct
     1261 tgtttactca cttgttcaag gtgctgcctt cgctatggct gacgtcgtaa acctacgtat
     1321 gcactcattc ggttcaatcg agttcttgac tcgtacacct attgcaatca gtgctggtat
     1381 tggtatggat atcgttaact tcgtttgggt aactgttctc tttgctgtaa tcatgtactt
     1441 tatcgcaaac ttcatgattc aaaaattcaa ctacgcaact ccagggcgca acggaaacta
     1501 cgaaactgct gaaggttcag aagaaaccag cagcgaagtg aaagttgcag caggctctca
     1561 agctgtaaac attatcaacc ttcttggtgg acgtgtaaac atcgttgatg ttgatgcatg
     1621 tatgactcgt cttcgtgtaa ctgttaaaga tgcagataaa gtaggaaatg cagagcaatg
     1681 gaaagcagaa ggagctatgg gtcttgtgat gaaaggacaa ggggttcaag ctatctacgg
     1741 tccaaaagct gacattttga aatctgatat ccaagatatc cttgattcag gtgaaatcat
     1801 tcctgaaact cttccaagcc aaatgactga agcacaacaa aacactgttc acttcaaaga
     1861 tcttactgag gaagtttact cagtagcaga cggtcaagtt gttgctttgg aacaagtaaa
     1921 ggatccagta tttgctcaaa aaatgatggg tgatggattt gcagtagaac ctgcaaatgg
     1981 aaacattgta tctccagttt caggtactgt gtcaagcatc ttcccaacaa aacatgcttt
     2041 tggtattgtg acggaagcag gtcttgaagt attggttcac attggtttgg acacagtaag
     2101 tcttgaaggt aaaccattta cagttcatgt tgctgaagga caaaaagttg cagcaggaga
     2161 tctccttgtc acagctgact tggatgctat ccgtgcagca ggacgtgaaa cttcaacagt
     2221 agttgtcttc acaaatggtg atgcaattaa atcagttaaa ttagaaaaaa caggttctct
     2281 tgcagctaaa acagcagttg ctaaagtaga attgtaatat acttgaggtt ggaagctgta
     2341 ttccaacctc ttattttggg agaaaagaat gaaattttta acactcaata ctcacagttg
     2401 gatggagaaa gaagcagagg aaaaattcca gattttgctt gaagatattc ttgaaaagga
     2461 ctatgatttg atttgtttcc aagaaatcaa tcaggagatg acctcgtcag aggtggaggt
     2521 taatgacctt tatcaagctt tgccagcagc tgagcctatt caccaagacc attatgttag
     2581 actcttggtt gaaaagttgt tagagcaagg gaaaaattac tactggacct gggcttataa
     2641 ccatatcggc tatgaccgct accacgaagg tgtggctatc ttgtctaaaa cacctattga
     2701 agccagagaa attttggttt cagatgtgga tgatccaaca gactatcata ctcgccgtgt
     2761 tgccctagct gaaactgtag tcgatggcaa ggagctagca gttgccagtg ttcatctctc
     2821 ttggtgggat aaaggtttcc aagaagaatg ggcacgattt gaggctgtct tgaaaaaatt
     2881 gaacaagcca cttttactag ctggagattt taacaaccca gcaggtcagg aaggttacca
     2941 agctatttta gctagtccat taggcttaca agacgcattt gaagttgctc aagagaaaag
     3001 tggtagctat actgttccgc ctgaaattga tggctggaaa gggaacactg aaccccttcg
     3061 aatcgattat gtctttacta ccaaagagtt agcggtggaa aatttacatg tcgtatttga
     3121 tggtaacaag agtccacaag tgagtgatca ctatggcttg aatgctatat taaactggaa
     3181 ataataactg aaaagaggtt ggaactataa aattccagcc ttttcttact agagaagcta
     3241 ctggaaatag cctaaataag tgagactact gtaatggaat aaaatatggt ataattgata
     3301 agatagatag aatcgaggat gttatgtcat ttacgaaatt tcaatttaaa aactatatta
     3361 gagaagcctt gaaggagtta aaatttacaa ctccaacaga ggtgcaagac aagttgattc
     3421 ctattgtttt ggcaggtcgt gacctagtag gagaatcaaa aacaggttca ggtaagactc
     3481 atactttctt gttaccgatt ttccagcaat tagatgaagc tagcgatagt gtacaagcag
     3541 tgattactgc accgagtcgt gagttggcta ctcaaattta ccaagtagcg cgtcagattt
     3601 cagctcactc agatgtcgaa gttcgtgtgg ttaattatgt gggtggtacg gataaggctc
     3661 gccagattga gaaattggaa agcaatcagc ctcatattgt tattggaaca ccaggccgta
     3721 tctacgactt ggttaaatct ggtgatttag ctattcataa agccaagaca tttgttgttg
     3781 atgaagcaga tatgaccttg gatatgggat tcttggaaac tgttgataag attgctggca
     3841 gtcttccaaa agacttgcaa ttcatggtct tctcagcgac tatcccacaa aaactgcaac
     3901 cattcttgaa aaaatactta tcaaatcctg ttatggagaa aattaagacc aaaacggtta
     3961 tttctgacac cattgataat tggttgattt cgaccaaggg acatgataag aatgctcaaa
     4021 tttaccagtt gactcagttg atgcagccgt atttggcaat gatttttgtt aacactaaaa
     4081 cgcgtgctga tgaattgcat tcatatctga ctgctcaagg cttgaaggtt gcaaaaatcc
     4141 atggagatat tgcccctcgt gaacgcaagc gaatcatgaa tcaggtgcaa aatctggatt
     4201 ttgagtatat tgtcgcaaca gatttagcag cgcgtgggat tgacattgaa ggtgtcagcc
     4261 atgtcatcaa tgatgccatt ccgcaagact tatctttttt tgttcatcgt gttggtcgta
     4321 ctggacgaaa tggcctacca ggtacagcta ttacccttta tcagccaagt gatgactcgg
     4381 atatccgtga gttggagaaa ttgggaatca agtttagtcc taagatggtc aaagacgggg
     4441 aatttcaaga tacctatgac cgtgatcgtc gtgccaaccg tgagaaaaaa caagataaac
     4501 ttgatatcga aatgattggt ttggttaaaa agaaaaagaa aaaagtcaaa ccgggttata
     4561 agaagaaaat tcaatgggcg gttgatgaaa agcgccgtaa aaccaagcgt gctgaaaatc
     4621 gcgctcgcgg tcgtgcagag cgtaaagcta aacgccaaac attttaatag aaattgttgg
     4681 agtattgagc tccaactttt ttatttatga gaacgaacta tctaaaccga aacactacat
     4741 taaagactgc aaattgcgat taaaaatggt ataatgataa agttatatag tcccgataag
     4801 atggtaggta tttattacga agagttttcc tatcagtact ttgtaactct ataacaatat
     4861 tttttaaggg gggacatttt tatgtcagaa cgtaaattat tcacgtctga atctgtatct
     4921 gaggggcatc cggataagat tgcagaccaa atttcagatg cgattttgga tgctatttta
     4981 gcaaaggatc cagaggcgca cgttgctgct gaaacagctg tatatactgg ttctgtccac
     5041 gtttttggtg aaatttctac aaatgcctat gtggatatta accgtgtggt tcgtgatacc
     5101 attgcagaga ttggttatac caatacagaa tatggatttt ctgctgagac ggtgggagta
     5161 cacccatctt tggtggaaca atctcctgac atcgctcaag gtgttaacga agccttggag
     5221 gttcgtggaa atgctgatca agatccactg gacttgattg gagcaggtga ccaagggctc
     5281 atgtttggat ttgcagtaga tgaaacagaa gagctcatgc cattgccaat tgcactcagt
     5341 cataaattgg ttcgtcgtct ggcagaactt cgtaagtctg gagaaattag ctatctccgt
     5401 ccagatgcaa aatcacaagt tacagttgag tacgatgaaa atgaccgtcc ggtacgtgta
     5461 gatacagtcg ttatttctac tcagcatgat ccagaggcca ctaatgaaca aatccatcaa
     5521 gatgtgatta acaaggtcat caaagaagtt attccatctt cttatcttga tgataagaca
     5581 aaattcttta tcaatccgac aggtcgtttt gtaatcggtg gtcctcaagg ggactcaggt
     5641 ttgactggtc gtaagattat tgtagatact tatggtggct actctcgtca tggtggtggt
     5701 gccttctctg gtaaagatgc gactaaggtg gatcgttcag cctcttatgc ggctcgctat
     5761 attgccaaga atatcgttgc agcaggcctt gctaagaagg cagaagtgca gttggcctat
     5821 gctatcggtg ttgcgcaacc tgtttctgtt cgtatcgata ctttcggtac aggaacagta
     5881 gctgaaagtc aacttgaaaa agcggctcgt caaatctttg accttcgccc tgcagggatt
     5941 atccaaatgc tggacctcaa gcgtccaatt taccgtcaaa catcggctta cggtcacatg
     6001 ggacgtacag atattgatct tccatgggaa cgtttggata aggtagatgc tttgaaagaa
     6061 gcagtaaaat aagattttaa gaggggaacg tcctctcttt tttatagttt ttaactatac
     6121 tgggatactg ttctgaaaat ccattttgcg aaagtagaga tttacatgta tagtagattg
     6181 aaactagaat agtacacctc aacttctaaa acattgttag caatcaattt gactgtcctg
     6241 atcgatttct cctgttcttg tttcatttta ctatatttct ttaaaaatga taaaggttga
     6301 gatttctcct cgtaatagat aatcatgggg atatttcaat ccaaagtttt attcgttatc
     6361 acttgactat tgcaaggttt tctagagcaa cagagtcatg gaatggactc atggttgaga
     6421 tttctccttg ttgcttggac ttcattcaaa agtctgttac ccaagccttg ttcaaacttc
     6481 caatacacta gctgttttca tagcatgact tctgtactag actttctttt ccgaataaat
     6541 agatagaacc acagaatcta gtaaacctag aattaaaatt atggtataat attagcaata
     6601 aaagaaatct ggaggattag aatcatggta tcaacgaaaa cacaaattgc tggttttgag
     6661 tttgacaatt gcttgatgaa tgcagcaggt gtggcttgta tgacgataga ggagttagaa
     6721 gaggtcaaaa actcagcggc aggaaccttt gttactaaga cagcgacctt ggacttccgt
     6781 caggggaatc ctgagccacg ctaccaagat gttccacttg gttccatcaa ctctatgggc
     6841 ttgccaaata atggcttaga ctattatttg gattatcttt tagatttgca ggaaaaagag
     6901 tcgaaccgaa ctttcttctt atctctggtc ggcatgtctc cagaggaaac ccatactatt
     6961 ttgaaaaaag ttcaagagag tgattttcgt ggtctgactg agctaaatct ttcctgtcca
     7021 aatgttccag gtaaacctca gattgcctat gattttgaga caacagaccg gattttggca
     7081 gaagtgttta cttacttcac caaacctctt ggaattaaat tgccacctta ttttgatatt
     7141 gttcactttg atcaagcggc agctattttc aacaaatatc cgctcaagtt tgtcaactgc
     7201 gttaactcta tcggaaacgg cctctatata gaagacgaat ctgtcgttat tcggcctaag
     7261 aatggttttg gtggaattgg tggagaatac atcaaaccga ctgctctagc caatgttcac
     7321 gccttttatc aacgtttaaa tcctcaaatc caaattatcg gaacaggtgg cgttctgact
     7381 ggtcgagatg cctttgaaca catcctctgt ggagcaagta tggtgcaggt gggaacgacc
     7441 cttcacaaag aaggcgtcag tgcttttgac cgcattacca atgaactgaa agcaatcatg
     7501 gtggaaaaag gctacgagag cttagaagat ttccgtggga aattgcgcta tattgactaa
     7561 attaaatcga aaaatctgaa gaaaggagag acgatgctag ccattgaaga aagtcagaag
     7621 ttgactttat caaatttacc gagcctgagc ctatttacag ggacagatca gggtcagttt
     7681 gaagtgatga agagtcaagt gttgaaacag attgggtatg attctgctga cctcaacttt
     7741 gcctactttg atatgaaaga agtagtttac aaggatgtgg aactggagtt ggtcagcctt
     7801 cctttctttg cggatgagaa aatcgtgata ttagattatt ttatggatat cacgactgct
     7861 aagaaacgct ttttgacaga tgatgagctt aagtcatttg aggaatacct tgacaatcct
     7921 tctccaacaa ccaagttgat aatctttgca gaaggaaagc tggatagcaa aagacggtta
     7981 gtcaaattac ttaagcgtga tgccaaggcc ttcgatgcag tagaagtaaa agaacaagaa
     8041 ttgcgccagt atttccaaaa gtggagtcag aaacaaggtc tgcagtttac caatcattct
     8101 tttgaaaatc tcctcatcaa gtcggggttt caatttagcg aaatccagaa aaatcttctc
     8161 tttttacagt cctataaggc ggattctgtt atcgaggaag aggatattgt taacgcaatt
     8221 cccaagacct tgcaggacaa tatttttgat ttaactcagt ttattctgac taaaaagatg
     8281 gatcaggcgc gcgatttggt gagagacttg accttgcaag gggaagatga aatcaaacta
     8341 attgcagtca tgctgggaca atttcggact tttactcagg tgaagatttt ggcggagtct
     8401 ggccaaacag aatcgcagat tgcaagtagt ttaggtagtt atctgggacg taacccaaat
     8461 ccttatcaaa tcaagtttgc attaagagat tcgagaggac tttctttgag ctttctgaag
     8521 caagctattt cctatttgat tgagacagac tatcagatta agacaggtct ttatgaaaaa
     8581 ggtttccttt ttgaaaaggc actcttacag attgctagtc aggtcaattg acatttgttg
     8641 aaactacaac ccgcgtcagg attatcttgt cgtgggtttc ttgctgattt gcttatctat
     8701 aagcaaaaaa cttgaaaaaa gtgaatgttt gcgttatcat tttcatatag aaagaaaaag
     8761 aggtattaca gatggctatt atcttaccag aacttccata tgcatacgac gctttggaac
     8821 catacatcga tgcggaaaca atgcatttgc accatgacaa acaccatcaa acttatgtca
     8881 acaatgccaa tgcagcttta gaaaaacacc ctgaaatcgg tgaagacctt gaagccttgc
     8941 ttgctgatgt agaatctatc ccagctgata tccgtcaagc acttatcaac aatggtggcg
     9001 gacacttgaa ccacgctctt ttctgggaat tgatgactcc cgagaaaaca gctccttcag
     9061 cagaactggc agcagcaatc gatgcaacat ttggttcatt tgaagaattc caagcagcct
     9121 tcactgcagc agcaacaact cgttttggtt caggttgggc atggttggtt gtcaacaaag
     9181 aagggaaact tgaagtgact tcaacagcaa accaagacac accaatctca gaaggtaaaa
     9241 aaccaatctt gggcttggac gtttgggaac atgcttacta cgtgaaatac cgcaacgtgc
     9301 gtcctgacta catcaaagct ttcttttcag taatcaactg gaataaagta gatgaattgt
     9361 acgcagctgc taaataatga tagttggagg gaagaattgt tcttctcttt tttaggttat
     9421 aagattctgg tctgacaaaa tcgttagact tttttcattt tttatgagaa acgtgctaac
     9481 tgctagaaat tatggtagaa taaagtatta agtaatcgtg gaaaaaggat atctatgcga
     9541 aaagaaattg cacctgaatt atacaactat aacaagtttc ctggtccgga gttccattta
     9601 cacggggaca aggtcgaaac ggaagggata gctttttcct tggttgaaaa tatcaaggat
     9661 gcctttgatg tgacgacttt taatcagcgt ttttcagaag tcttaaccaa gtttgattat
     9721 atcgtggggg actggagcaa cgaacagctt cgcctacgag gtttttacaa ggatgaccga
     9781 acagaagaaa aacttgaaaa aatcagtcgt ttacaagact accttttaga gtattgtagt
     9841 tatggttgtg cctattttgt cttagaaaat gaagccccta agcgagcatc atttgacaag
     9901 aaaatgcgta agacggaaga agaaacacct tctagaaaag gaaagaaacc gactcaaact
     9961 aaacgaaagt cgaatgcaga taagaaaaat agacgtcgtc agaaagacca gcattctcag
    10021 aaagaggaca aggaacaacg tcattttgtc attcgtcaga agtgagtaga gaataaggag
    10081 aagaaatgaa accctcaatt catagtttgg tacatcaaac catgcaggaa tgggtattgg
    10141 agcagggaga aaagaaattc cgtgcagatc aaatctggga atggctctac cgtaaacgtg
    10201 tgcagtcatt tgaagaaatg accaaccttt ccaaggattt gattgctaag ctcaatgacc
    10261 agtttgtggt caatcccttg aaacaacgta ttgtgcaaga gtctgctgat ggtacagtca
    10321 aatatctctt tgaattgcct gacggtatgt taattgagac tgtactcatg cgtcaacact
    10381 atggtttatc tgtctgtgtg accactcagg tcggctgtaa tatcggttgt accttctgtg
    10441 cctctggttt gattaagaag cagcgtgacc tcaataacgg tgaaatcgta gcgcaaatca
    10501 tgctggttca gaaatacttt gctgatgaag gggttcaggt caatcttgcc gtgtctcttc
    10561 acgcacccaa caatgaactt cgttcaagta tcatgaagat taaccgtgcc tttccgattg
    10621 aaaaactctt tgctgctatt gaatactaca ttgagacaac aaaccgtcgt gtaacctttg
    10681 aatatatcat gcttaatgaa gtcaacgacg gtgtagaaca agccttggaa ttggctgaat
    10741 tgctcaagaa catcaagaaa ttgtcttatg taaacttgat tccttataac ccagttagtg
    10801 agcatgacca atatagccgt agtcccaaag agcgcgtgtt ggctttctat gatacgctta
    10861 agaaaaaagg ggtcaactgt gtggttcgtc aagagcatgg tacagatatt gacgcagctt
    10921 gtggacaatt gcgctctaac acaatgaaac gtgaccgcca gaaggcagtc gcagcggtaa
    10981 atccttaatg atgaagaaga cttataatca tattttggtc tggggagtca ttttctatag
    11041 catttgcatt gtctgttttt gctttactcc tcaagaacaa tctaccgtgg gagtgggaac
    11101 tccaggtatt cagcatcttg gacgcctggt ttttcttttg actcctttca attctctctg
    11161 gaaactgggc gaagtgagtg acattggaca attatgttgg atttttttac aaaatgtcct
    11221 caatgtcttc ttgttttttc ctctgatttt ccaactcctt tatctatttc caaatttgcg
    11281 gaaaacaaaa aaggtccttc tttttagttt tcttgtgagt cttggaatcg agtgtacgca
    11341 attaatcttg gactttttct ttgatttcaa tcgcgtcttt gagattgatg atttgtggac
    11401 caatactttg ggtggctatc tggcttggct cctttataaa cgattacata aaaacaagat
    11461 aaggaattaa aatgagtatt ttagaagtta aaaatctgag tcacggtttt ggtgaccgtg
    11521 caatttttga agatgtgtcc ttccgtctcc tcaagggaga acatatcggc ctggtcggtg
    11581 ccaatggtga aggaaaatca acctttatga gtatcgtgac tggtaaaatg ctgccagatg
    11641 aaggaaaggt tgagtggtcc aaatatgtga cggctggtta cttggatcag cactctgtcc
    11701 ttgctgaaag acagtcggtg cgtgatgttc tccgtacggc ttttgatgag cttttcaaag
    11761 ctgaagctcg tatcaatgac ctctatatga aaatggctga agacggcgcg gatgttgatg
    11821 ctctcatgga agaagtagga gaacttcaag accgtctgga gagtcgtgat ttctatacct
    11881 tggatgctaa gattgacgaa gtagcgcgtg ctcttggtgt tatggacttt ggcatggata
    11941 cggatgtaac ttctttgtca ggtgggcaaa gaaccaaggt gcttttggca aaacttctcc
    12001 ttgaaaagcc tgatatcttg ctgttggacg agccgaccaa ctacttggat gctgagcata
    12061 ttgattggct caagcgctat ctccaaaact atgagaatgc ctttgttctc atttcgcacg
    12121 atattccatt cctcaatgac gttattaata ttgtctatca tgtggaaaat caacagctga
    12181 cgcgttactc tggtgactac taccagttcc aagaagttta tgctatgaag aaatctcagc
    12241 tagaggcagc ctacgaacgc cagcagaaag agattgcaga cctcaaggac tttgtggctc
    12301 gtaataaagc ccgtgttgca actcgtaata tggctatgtc tcgtcaaaag aaattggata
    12361 agatggatat tatcgaactc caaagtgaga aaccaaaacc atcctttgat ttcaaaccag
    12421 ctcgtacacc agggcgcttt atcttccaag ccaagaactt gcaaattggt tacgaccgtc
    12481 ctcttactaa gcctttaaat cttaccttcg aacgcaatca aaaggttgcg attattggtg
    12541 ctaatggtat tggaaaaaca actctcttga agagtctctt gggcattatc tcgccaatcg
    12601 ctggggaagt ggagcgtgga gattatttag aacttggtta ttttgagcag gaagtagaag
    12661 gtggtaatcg ccaaactcct cttgaagctg tctggaatgc ctttcctgcc cttaatcaag
    12721 cagaagtccg tgcagccctt gcccgttgtg gtttgacaac caaacatatt gaaagccaga
    12781 ttcaagtatt atcaggggga gagcaagcca aggttcgttt ctgtctcttg atgaatcgtg
    12841 aaaacaacgt tttagtgctg gacgagccga ccaaccattt ggatgtggat gcaaaggatg
    12901 agctcaaacg cgctctcaaa gaatacaggg gatctatcct tatggtctgc cacgagccag
    12961 acttttatga aggttggata gaccaaatat gggattttaa taatttaact taagaatgag
    13021 agtaaaagaa atacggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
    13081 aggacaaaat tcgtcctttc ttttttgatg ttcagagcga taaaaatcct ttttttgaag
    13141 ttttcaaagt tccgaaatcc aaaggcgttt cgtttgataa gtttgatgag attattggtc
    13201 gcttccaatt ttgcgttgga ataaggtaat tgaagagcgt tgacgatttt ctctttgttc
    13261 tttagaaagg ttttaaagac agtctgaaaa ataggatgaa cctgcttcag attgtcctca
    13321 atgagtccga aaaatttctc tggttcctta ttctgaaagt gaaa
//
LOCUS       DAYYQN010000051        12868 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYYQN010000051 DAYYQN010000000
VERSION     DAYYQN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.052"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            112..267
                     /locus_tag="ACHBRQ_001578"
                     /pseudo
     CDS             112..267
                     /locus_tag="ACHBRQ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            467..3742
                     /gene="rexB"
                     /locus_tag="ACHBRQ_001579"
     CDS             467..3742
                     /gene="rexB"
                     /locus_tag="ACHBRQ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HFM9593215.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3739..7389
                     /gene="addA"
                     /locus_tag="ACHBRQ_001580"
     CDS             3739..7389
                     /gene="addA"
                     /locus_tag="ACHBRQ_001580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9593216.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TIRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7604..8782
                     /locus_tag="ACHBRQ_001581"
     CDS             7604..8782
                     /locus_tag="ACHBRQ_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593217.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLLLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            8991..>12868
                     /locus_tag="ACHBRQ_001582"
     CDS             8991..>12868
                     /locus_tag="ACHBRQ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HFM9593218.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGT
                     NNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHA
                     DGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLID
                     KSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLW
                     DPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEI
                     LTKDSTVQRGEVLEDAQIKEKKATFVSKNT"
BASE COUNT         4266 a         2454 c         2706 g         3442 t
ORIGIN      
        1 taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat tttagcatcc
       61 acggtgtact attatagttt caatttacta tagttcaatc attctatcaa gaatattgaa
      121 tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc cgatgttgat
      181 ggcgataaat cagacaatct accagtcgac attaagatac tgggacaaca cattgaagtg
      241 tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg cttttttatt
      301 atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa gtatttaaga
      361 tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat ttacgaggct
      421 aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga aattacttta
      481 tactgatatt cggacttctt tgacagaaat tctaacaaga gaggcagaag agctagttgc
      541 agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa aggaacgcgc
      601 cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc gctttgctca
      661 aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg atgatatcgg
      721 tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact tgcgtgttta
      781 tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt accatgagat
      841 gaccaaatcc cagatgagtt ttttggactt ggagaattta acagatgagg ataagagggc
      901 ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaaggtc agttagccca
      961 gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa gtagtgattt
     1021 taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg aagagcgggt
     1081 tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg ctagtaagaa
     1141 agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa aatttctcca
     1201 tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc atgagaagat
     1261 ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt cagaactcgc
     1321 tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt tgacgcaaaa
     1381 ggaggagttg gagctagtag cccgtagtat tcgtcagaaa ttacatgaga actcagacct
     1441 gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt tatctctcaa
     1501 aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag ccatggctca
     1561 tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc gttttcgtca
     1621 ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc agtctgatat
     1681 tgatgctttt gagcaatata tccgctatct tggtatcaat ggcttgccag cctttcagca
     1741 aatcttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg tcctccgcct
     1801 gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg ctgaaaacct
     1861 cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt tacaagattt
     1921 gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga aggctttctg
     1981 ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc tggaagactt
     2041 cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc cagcaacagt
     2101 ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg actttgtcta
     2161 tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca gtcttctgac
     2221 agatgaagaa aggcaaaacc taaaccaagc gaccgaagaa ggcgttcaat tactgattgc
     2281 cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca attctgctcg
     2341 taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta aggaatctgc
     2401 ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga tgaatcacaa
     2461 aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc tggttgccta
     2521 tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca aggttctgtc
     2581 gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta tcccaaccag
     2641 tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg ccaaacagga
     2701 gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca gttatttcct
     2761 acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc gtagtcacgg
     2821 gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag attcctttga
     2881 ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg aagctattta
     2941 tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg cacggacgac
     3001 tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag caaattttgg
     3061 tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg tacgaggcaa
     3121 ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag actacaaatc
     3181 cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc agttaccaac
     3241 ctatcttgct gccctaaaaa gagaagggaa gcagaacttt ttcggcgcca tgtacttgga
     3301 aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag cagtggtaga
     3361 agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca gttatttagg
     3421 cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc agctcctact
     3481 ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag gccggttcgc
     3541 catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc aacatcaggc
     3601 tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag aaaaattgga
     3661 cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt ttgaaaaaat
     3721 aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga gattcaaaaa
     3781 ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga acaaatcgaa
     3841 gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc tggaaagacc
     3901 tttgtcatgg cagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat ttctcaactc
     3961 tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt agagaaaaaa
     4021 atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt gggtcgccag
     4081 ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca aaaattcctt
     4141 ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca aaaccaaagc
     4201 gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca ttaccaaggt
     4261 aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg caaggacgaa
     4321 cggggtctgc ggcagcaagt ctataaaatc tatgacttcc tccaatccac cagtaatcct
     4381 caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt taccagtgaa
     4441 aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag ctttttccgt
     4501 taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga aaatgttcag
     4561 ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc ttatcaggca
     4621 gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct gactaatgcc
     4681 agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag aaagacccag
     4741 tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca agaacgttat
     4801 catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga ttttgtagag
     4861 gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat cagccattac
     4921 accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga gcgcttccat
     4981 gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat gctggaattg
     5041 ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat ctatcgtttc
     5101 cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca aaatccccaa
     5161 gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt gctgtcagca
     5221 accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa ctatgataac
     5281 aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa caaggcagaa
     5341 tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac agaaacgaaa
     5401 ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca agaaaaaggt
     5461 gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga ccagattctc
     5521 ctcgccctgt ctgagtacgg gattcctgtc aaaactgacg gagagcaaaa caattatctc
     5581 caatccctag aagtgcaagt catgctagac actattcgtg tcattcacaa tcccctgcaa
     5641 gactatgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga ggatgagcta
     5701 gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta tgagaaactg
     5761 gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc tctagctgaa
     5821 aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa aacccactct
     5881 ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta tgttggggct
     5941 ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg tgctgatcaa
     6001 tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga ccaagtctta
     6061 gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc agtagagctc
     6121 atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct caatatggat
     6181 caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca gaatggtctt
     6241 ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta tcctaaaacc
     6301 atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt acagctagca
     6361 agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga gaaaaagctc
     6421 tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc agcagccaaa
     6481 aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga ttggctttgg
     6541 gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt tattggcgaa
     6601 gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct ccaagatagc
     6661 tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat gctgaaggag
     6721 gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt tcaaacccca
     6781 agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga gattgctggt
     6841 caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc aaccaaagaa
     6901 aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag aattgacctc
     6961 agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca aactagccaa
     7021 gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac agtactcggt
     7081 caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc catgctcaaa
     7141 cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg ctatctgctt
     7201 tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga accaagtcaa
     7261 ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc acgagcctat
     7321 tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt tcaagttgta
     7381 aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg ccttgctgta
     7441 tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagctgac
     7501 ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgattgcgg ctagctttct
     7561 agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac ttccagttag
     7621 aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg aacaagctac
     7681 caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca attgtgtctg
     7741 tggaaatgcc atcaaatacg cttatgaact ctttaacggt gtcacaggcc aacgcctctt
     7801 tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc agcaattgga
     7861 agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc agaaaaagga
     7921 aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc tctgggaaaa
     7981 aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact accagctttt
     8041 actagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa aggctcggat
     8101 ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg atgaccaact
     8161 ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc gtatccaggc
     8221 agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca atctggttct
     8281 agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag agaccctgac
     8341 tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag aacgggctat
     8401 caaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca gtcaggatcc
     8461 aattgaaaga aaacagaagc agacttggct cctcaattcc tattttcgtg agcttcctga
     8521 agaaaaagct cgattttcta ggctcttatt agaatatcga aaaagtggtg aagtaccctt
     8581 ttcaactgaa tacctgtccg atcatctcat cgactttttc tataaaatga aagcctttga
     8641 atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc aggaagacca
     8701 tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc tcaaaccatt
     8761 tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt aggtgttatt
     8821 gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa gacttttttg
     8881 taaattaaga gtataattaa ggtatactgc cttttctata gataatagat aatacactaa
     8941 tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata atggaaaagt
     9001 attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga cttgtatctg
     9061 caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg gatgctcaag
     9121 aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca gaagaaaaga
     9181 agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact tattatcttg
     9241 tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga agcaagaatg
     9301 agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta atttttgctg
     9361 tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt gcgggaatgg
     9421 gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta aattacaata
     9481 cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt tcaggttaca
     9541 cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta agtaatcaag
     9601 aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt acaccaaatt
     9661 ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt tcttcaacta
     9721 agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc aatccaagaa
     9781 aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag aatctagaaa
     9841 cgaagaaaga ggagaagatt tctccaaagg aaaagactgg ggtaaataca ttaaatccac
     9901 aggatgaagt gctatcaggt cagttgaaca aaccggaact cttatatcgt gacgaaacta
     9961 tagaaacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta gcagaaggaa
    10021 ctgtaagagt aaaacaagaa ggtgcattag gtaagaaagt tgaaatcgtc agaatatttt
    10081 ctgtaaacaa tgaagaagtt tcacgagaaa ttatttcaac ttcaacgact gcgcctgttt
    10141 caagaatagt cgaaaaaggt actaaaaaag ctcaagttat aaaggaacaa gctgagactg
    10201 gtgcagaaca taaggaagta cagtctggag ctattgttga acccgcaatt cagcctgaac
    10261 taccagcagc tgtattaacc gacaaaggtg agtcagcagt ccaaccagag ttgccagaag
    10321 ctgtagtaag tgataaaggc gtaccagaag ttcaaccagc gttaccagag gcagtcatga
    10381 ctgacaaggg tgatccagag caggtagaac cgctaccaga atatacagga gtacaagctg
    10441 gagctattgt tgaacctgaa aaagttgaac cggaatatgc tggagtacaa gctggcgcta
    10501 ttgttgaacc agagcaggta gcaccactac cagaatacac aggggtgcaa gccggagcga
    10561 tagtcgagcc agagaaagta gaagcaccga aggaatacac aggagtgcag gctggagcga
    10621 tagtcgaacc agagaaggta gaagcaccga aggaatacac aggagtgcaa gccggagcaa
    10681 tagtagaacc agaaaaagta gaaccatcaa aagaatacac aggggtgcag gctggcgcta
    10741 ttgttgaacc agagcagata gcaccactac cagaatacac aggggtgcaa gccggagcga
    10801 tagtcgagcc agagaaagta gaagcactga aagaatatac tggtaaaatc gaacaaccta
    10861 gtgcagaaga tactaaacca aataatgaaa atactaatac accagaagaa atgagtattc
    10921 agaaaaaatc tagtgcatta attaatatga actttattac agattcgtcg aaggttactg
    10981 gagtaggatc agctactttt attgcaccaa atgtactttt aacagtagca cataatttca
    11041 ttaataattc tactgataat actactggcg aatttagagg tgataaatct aaaaacgtat
    11101 atgagtgggt gactcctgat ggacaaaaag gaacatttac tgctaacaat attcattttt
    11161 ataataaaaa agattatcca aaaggattta tctatgattt agcagtgatt aagctacctg
    11221 aaacaactgg cagagaacat gtagaattag ttaaaaatta ttcgaaagta aatcttaatg
    11281 acaaactaaa tgttcatgga tatccagctg ggaaatatac gcacttaaaa gatgctacgg
    11341 tagaaatgga acaagaatat gcgaataaca cctacggtgt tcaatatcaa ggtggaaatc
    11401 ctggtatgag tggtggagga atatttaatg ctaatggtga agttattggt gtgcatcaaa
    11461 atggagcaca aaatcgctca ggcggattga ttttatctcc tacacaatta gcttggatta
    11521 agagtattat agcaggtaat gaaattccac cagtatacga tgaactttat cgtcataaag
    11581 atgagaaaaa agatgacgct aaagatgaaa aagaagttat taagaaatta gaacttagaa
    11641 atatatcttc tgtagaacta tattcaaaag atggtaataa gtatcgacat gtaacttcac
    11701 ttgcttcttt accaagtaat gcagaaaatt actttatgaa agttaaatca gagaacttta
    11761 aagatgtgat gctaccagtt acaagcatta ctaatgatac taaagataat agagatgttt
    11821 ataagatagt agcaagtgca aatagtctga tacaacatga aaataataat gtactagaaa
    11881 attatacata ctatttacca aaaactcagc aaagtgaaac tggggtatat acatcattta
    11941 aaaatttagt agatgcaatg aatagcaatc ctaatggcac attccgttta ggagcaacta
    12001 tggatgctcg tgaagttgaa cttccagatg gtcaagaaag ttatgtgaat aatgtattcc
    12061 atggaatatt agtaggaaca aataatgaga aatattatgc tatttataat ttgaaaaaac
    12121 cattatttgg ggaattaaat ggtgctacag tagaaaaact ttcactaaaa gatgttaata
    12181 tttccgcaaa ggacgatact gcaacacttg cgaaagaagc aaataacaat actcatattg
    12241 ataacgttca tgctgatgga gcgatagctg gggaacgtag cataggagga ttagtatcac
    12301 aagttaataa ttctactatt tctaacagta gctatacagg tagaataact aatacatata
    12361 aaacagttgc tagttatcaa atcggaggtt tagtaggcaa actttctggt ccgaggggat
    12421 taatagataa gtcgttcgca tcaatcgatt tatcttcaaa tgcaacacaa ggagaccagt
    12481 ctattggtgg aatagttggt gcagtagaaa atagtgcgct tattagtaat agttatgctg
    12541 aaggaaatct aaataatgtt caacgttttg caaacgttgg tggagtagtt ggtaatcttt
    12601 gggatccggt tggaggatta gaaaaatccg gtcgactttc taatgtacta agcgatgtta
    12661 atgtaacgaa tggtaatgct attgcaggtt ataattttaa tggtataaaa gcaaatggca
    12721 catacagtaa taaaaataat aaggttgtga acgtagtaca agaagatgat gaaatcttaa
    12781 ctaaggattc tactgttcaa agaggagaag tattagaaga tgcacaaata aaagagaaaa
    12841 aagctacttt tgtatctaaa aatactat
//
LOCUS       DAYYQN010000052        12631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYYQN010000052 DAYYQN010000000
VERSION     DAYYQN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.053"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            444..1487
                     /locus_tag="ACHBRQ_001583"
     CDS             444..1487
                     /locus_tag="ACHBRQ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HFM9593219.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1654..3741
                     /gene="topA"
                     /locus_tag="ACHBRQ_001584"
     CDS             1654..3741
                     /gene="topA"
                     /locus_tag="ACHBRQ_001584"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9593220.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3847..4206
                     /locus_tag="ACHBRQ_001585"
     CDS             3847..4206
                     /locus_tag="ACHBRQ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFM9593221.1"
                     /translation="MRLIYLIIGFLSLGLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4299..4931
                     /locus_tag="ACHBRQ_001586"
     CDS             4299..4931
                     /locus_tag="ACHBRQ_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9593222.1"
                     /translation="MIYEFCAENMTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4975..5631
                     /locus_tag="ACHBRQ_001587"
     CDS             4975..5631
                     /locus_tag="ACHBRQ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9593223.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5707..6744
                     /locus_tag="ACHBRQ_001588"
                     /pseudo
     CDS             5707..6744
                     /locus_tag="ACHBRQ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            6756..7793
                     /gene="leuB"
                     /locus_tag="ACHBRQ_001589"
     CDS             6756..7793
                     /gene="leuB"
                     /locus_tag="ACHBRQ_001589"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFM9593224.1"
                     /translation="MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7790..8059
                     /locus_tag="ACHBRQ_001590"
     CDS             7790..8059
                     /locus_tag="ACHBRQ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9593225.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8063..8422
                     /gene="leuD"
                     /locus_tag="ACHBRQ_001591"
     CDS             8063..8422
                     /gene="leuD"
                     /locus_tag="ACHBRQ_001591"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFM9593226.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8584..9207
                     /locus_tag="ACHBRQ_001592"
                     /pseudo
     CDS             8584..9207
                     /locus_tag="ACHBRQ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899133.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            9373..11304
                     /locus_tag="ACHBRQ_001593"
     CDS             9373..11304
                     /locus_tag="ACHBRQ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFM9593227.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQTVADDNEGDENDDADY"
     gene            11291..12607
                     /locus_tag="ACHBRQ_001594"
     CDS             11291..12607
                     /locus_tag="ACHBRQ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFM9593228.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
BASE COUNT         4035 a         2130 c         2706 g         3760 t
ORIGIN      
        1 ttgagaaagg agaaatgatt gtgattgatg ttggctaggt tatgttcaat gattcctacc
       61 gtctcaaatc ttgtcagtaa ggaaaaataa attcttcaaa agtagagatt acaaggcttg
      121 tttaagaaag aattcaaaga ccttgacaaa taaaaataaa atggttatta taaaaaatgg
      181 tctaaaatag atgatgatac ttttcgaaaa tctttttacg tcagctcagc tttgccttgc
      241 tgtgttttga gcaagctacg gttagcttcc gagtttgatt ttcatttact agaaatgaaa
      301 ctgatgagag atatcagtag acatttgagt caggatatta tggaaaatga taaaaagagc
      361 tcgtgagatt ggcatatcag actactaaag tattgagttt gttaggattt tagcgactag
      421 ttagctggga aaggaagata tttgtgacaa ataataaact gtattcgttg atagaattta
      481 gaaataaaat atatgaagaa ttagaacttt ccagaagtga tttagcgatt ttactatgtg
      541 ccatgcttat cgcctctatc ggattaaata tggattcgac tcccgtgatt attggagcca
      601 tgttaatctc tcctttgatg acacctattc tgggagtggg gctctctcta gctatatttg
      661 attttaaatt gttaagaaaa tcttttaaaa tattagctat tcaaattctt gccagtctaa
      721 tagcttcaac actttatttt tatctttctc ccatttcgta tgctagttcg gagattgttg
      781 ctagaacctc tccgactatt tgggatgttc tcattgcttt tgtaggaggg atagcaggta
      841 ttattggtgc taggaaaaaa gagaccaata atattgttcc tggtgttgct attgcaaccg
      901 ccttgatgcc tcctctttgt acagtaggtt atgctattgc ttctgctaat ctaaaattta
      961 tcataggctc ctcttaccta ttcctcatca attgtagctt tattgtcatt gcgacttata
     1021 taggtgttag gttgatgatg gttaagaaac attattttaa agataatgaa gaagactcta
     1081 aaatgcgtag gattttgctt ctagttgctg ttttgctgat gattccgagt ttcatctctg
     1141 caacgacttt agtgagagaa acgttgaaaa aagagtccct taagaaattt atatcagagc
     1201 agtttcaggg gtataatatt ttgaaaaaaa cctattctaa aaagactcat accctaaagc
     1261 taaccatttc aggaaattat ttgacagaag aagaactcga tatgatttca agtaagagag
     1321 gtgactatgg tttaagtgat gtttctgttc aagtttcaca attgtctgat tcagaacaac
     1381 ttagtaagga agagctggtg gagtatttct tccagtatat caaggataag gaagcaaaag
     1441 aaaaggagaa agccaataaa tttgatacag agtctgagga gcaataattt cttgagaata
     1501 gctggtttct ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt ttaagtataa
     1561 aacttttctt ctattatagt agctaagtct tgacagggtt caaaaaatgg tttacacttt
     1621 ataaagttta ttactttgaa aaggtgtgat actgtggcta cggcaacaaa aaagaaaaaa
     1681 tcaacagtta aaaaaaatct agtcatcgtg gagtcgcctg ctaaggccaa gacgattgaa
     1741 aaatatctag gcagaaacta caaggtttta gccagtgtcg ggcatatccg tgatttgaag
     1801 aaatccagta tgtccgtcga tattgaaaat aattatgaac cgcaatatat caatatccga
     1861 ggaaaaggcc ctcttatcaa tgacttgaaa aaagaagcta aaaaagctaa taaagttttt
     1921 ctcgcgagtg acccggaccg tgaaggagaa gcgatttctt ggcatttggc ccatattctc
     1981 aacttggatg aaaatgatgc caaccgtgtg gtcttcaatg aaatcaccaa ggatgcagtc
     2041 aaaaatgctt ttaaagaacc tcgtaagatc gatatggact tggtcgatgc ccaacaagct
     2101 cgtcggatct tggatcgctt ggtagggtat tcgatttcgc ctattttgtg gaagaaggtc
     2161 aagaagggct tgtcagcagg tcgcgttcag tccattgccc ttaaactcat cattgaccgt
     2221 gaaaatgaaa tcaatgcctt ccagccagaa gaatactgga cagttgatgc tgtctttaaa
     2281 aagggaacca aacaatttca tgcttccttc tatggagtag atggtaaaaa gatgaaactg
     2341 accagcaatg acgaagtcaa ggaagtcttg tctcgtctga ctagtaaaga cttttcagta
     2401 gatcaggtgg ataagaaaga gcgcaagcgc aatgctcctt taccctatac cacttcatct
     2461 atgcagatgg atgctgccaa taaaatcaat ttccgtactc gaaaaaccat gatggttgcc
     2521 caacagctct atgaaggaat taatattggt tctggtgttc aaggtttgat tacctatatg
     2581 cgtaccgatt cgactcgtat cagtcctgta gcgcaaaatg aggcggcaag cttcattacg
     2641 gatcgttttg gtagcaagta ttctaagcac ggtagcaagg tcaaaaacgc atcaggtgct
     2701 caggatgccc atgaggctat tcgtccgtca agtgtcttta atacaccaga aagcatcgct
     2761 aagtatctgg acaaggatca gctcaagcta tataccctta tctggaatcg ttttgtggct
     2821 agccagatga cagcggccgt ttttgatacc atggctgtta aattgtctca aaatggagtt
     2881 caatttgctg ccaatggtag tcaggttaag tttgatggtt atcttgccat ttataatgat
     2941 tctgacaaga ataagatgtt accggacatg gttgttggag atgtggtcaa acaggtcaat
     3001 agcaaaccag agcaacattt cacccaaccg cctgcccgtt attctgaagc aacactgatt
     3061 aaaaccttag aggaaaatgg ggttggacgt ccatcaacct acgcgccaac cattgaaacc
     3121 attcagaaac gttattatgt tcgcctggca gccaaacgtt ttgaaccgac agagttggga
     3181 gaaattgtca ataagctcat cgttgaatat ttcccagata tcgtaaacgt gaccttcaca
     3241 gctgaaatgg aaggtaaact ggatgatgtc gaagttggaa aagagcagtg gcgacgggtc
     3301 attgatgcct tttacaaacc attctctaaa gaagttgcca aggctgaaga agaaatggaa
     3361 aaaatccaga ttaaggatga accagctgga tttgactgtg aagtgtgtgg cagtccaatg
     3421 gtcattaaac ttggtcgttt tggtaaattc tacgcttgta gcaatttccc agattgccgt
     3481 catacccaag caatcgtgaa agagattggt gttgagtgtc caagctgtca tcagggacaa
     3541 attattgagc gaaaaaccaa gcgtaatcgc ctattctatg gttgcaatcg ctatccagaa
     3601 tgtgaattta cctcttggga caagcctgtt ggtcgtgact gtccaaaatg tggcaacttc
     3661 ctcatggaga aaaaagtccg tggtggtggc aagcaggttg tttgtagcaa aggcgactac
     3721 gaggaagaaa agattaaata agaggagggg cccgaaagta aatttcaggc tccttttggt
     3781 tagaacttga caaaatacat cattttatgc gaaactagaa gaagattatt ttaactagga
     3841 gaagttatgc gtcttatcta tctgataatt ggttttttat cactgggctt ggctattgtt
     3901 ggggttgttt tacccttgtt gccgacaacg cctttccttt tgttgtctat tgcttgtttc
     3961 tccagaagtt ccaagcgttt cgaagactgg ctttatcata ccaagctcta tcaaacatat
     4021 gtagctgatt ttcgcgagac taagtctatt acgcgtgaac gaaagaaaaa aatcatcgtc
     4081 tctatctacg tcttgatggg aatttctatt tattttgcac ctcttttacc agtcaaaatc
     4141 ggtctgggtg ctttgaccat ctttatcact tattatctct tcaaggtcat tccagacaaa
     4201 gaatagttaa aacagtagtt atttgccttg ataaaattga aagcatattc ataacaatat
     4261 gatataataa atttgaagta atattcaagg agaatcaaat gatttacgaa ttttgtgctg
     4321 aaaatatgac tttacttgaa aaagcgatgc aggctggagc tcgtcggatt gaactctgtg
     4381 ataatctagc agttggtggg acaacaccca gctatggagt gactaaggca gcggttgaac
     4441 tggcagctaa ctacgataca accatcatga ccatgattcg gccacgtggt ggtgactttg
     4501 tctataatga cctagaaatt gctatcatgc tagaagacat tcgtttgact gctcaggctg
     4561 gaagtcaagg ggttgtattt ggagctttaa ctgctgataa aaagttggat aagcctaatc
     4621 tggaaaagtt aattgctgca tcaaaaggaa tggaaattgt ctttcacatg gcctttgatg
     4681 aactaagtga tgaagatcaa ccggaagcta ttgactggct cagtcaagcc ggtgtcactc
     4741 gtatcctaac tcgtgctggt gtgtctggcg actccttaga aaaacgtttt gttcactatc
     4801 acagaatttt ggagtacgct aaaggtaaaa ttgaaattct accaggtggg gggattgacc
     4861 ttgaaaaccg tcaaaccttt atcgaccagg tgggggtaac acaattgcat ggtactaagg
     4921 ttgtttttta aaaaatagaa aggaactgct agcttttggt agcagttttc acttatgttt
     4981 gaaattttta aatcctatca atttaatcaa gaaaaggctc atgattatgg ttttatagaa
     5041 aatagcgaag tctggacata tagttgccag attttgcaag gtgactttgt catgactgtg
     5101 tccatcactg ctgataatgt gaactttcaa gtctttgacc aagagactgg tgacctctat
     5161 cctcacgttc atatggaaag catgagggga agttttgtcg gaaatgtccg tgaggcttgt
     5221 ctggagattc tttaccagat tcggaaggct tgttttgatg tgcaagattt tatctgtcat
     5281 cagactaagc gtatcatgac tcaagttcag gaaaagtatg gaaaccagtt ggagtatctg
     5341 tgggaaaaat cgcctgatac agctgtattg cgccatgaag gcaatcaaaa gtggtatgcc
     5401 gtcttgatga aaatctcttg gaataagctg gaaaagggca gagaaggaca agtggaagca
     5461 gtcaacctca agcatgacca agtagctaat ttgctttcac aaaaggggat ttatccagcc
     5521 ttccatatga gcaagcgcta ctggattagt gtgtcccttg atgatacttt atcagatgaa
     5581 gaagtactgg aattgataga aaaaagttgg aacttaacct ctaaaaaatg aaatatttta
     5641 ataattttca tgaactttca attagctaaa tattctttac tgaagaaatt ttcagaaaat
     5701 atagaattta ccacaccaga ggaatatggt gccatcttca aatacctgat tgagaatgtc
     5761 aagacggatc gtcagatcat ctattcgcct cactgtcatg atgaccttgg aatggcagtg
     5821 gcaaatagcc ttgctgctgt caagaatggt gcaggacgtg ttgaagggac tatcaatggt
     5881 attagggagc gagctggaaa tgctgctttg gaagaaattg cagtggctct caatattcgc
     5941 caagattact accaagtaga aaccagtatt gtcctaaatg agaccatcaa tacgtcagaa
     6001 atggtttctc gcttctctgg tattccagtt cctaaaaaca aagccgtcgt tggtggcaat
     6061 accttctccc acgaatctgg tattcactaa gatggagtcc ttaaaaatcc tctcacttat
     6121 gagatcatca cacctgaatt ggttggtgtt aagattccgc ttggaaaatt atctggtcgc
     6181 catgcttttg ttgagaaact gagagaattg gccctagatt ttacagaaga ggatatcaaa
     6241 tcactctttg ctaagttcaa ggcactggcc gataagaagt aagaaatcac agatgcagat
     6301 attcgagctt tggtagctgg aaccatggtt gaaaatccag aaggcttcca ctttgatgat
     6361 ttacaacttc aaactcatgc agataatgac attgaagcgc tcgttagcct agccaatatg
     6421 gatggtgaga aagtcgaatt taatgcgaca gggcaaggtt ccgttgaagc aatctttaac
     6481 gctatcgata agttctttaa ccaatctgtt cgtttggtgt cctacactat cgatgcagta
     6541 acagatggaa tcgatgccca ggatcgggtt ttggtcactg ttgaaaacag agatacagaa
     6601 accatcttta atgcagcagg gcttgatttt gatgtgttga aggcttctgc tattgcctat
     6661 ataaacgcta atacctttgt tcaaaaagag aatgcaggtg agatgggatg cagtgtttct
     6721 taccatgata tgcctagtgt gtaaaggaga aggctatggc aaagaaaata gtagctctag
     6781 caggagacgg aattggccca gaaatcatgg aggttggttt agaagttctg gaggctctag
     6841 ctgaaaaaac aggttttgac tatgagattg acagacgacc gttcggaggt gcagatattg
     6901 atgcagcatg gcctccctta cctgatgaaa cccttaaggc aagtagggaa gcagatgcta
     6961 tcctactagc agctatcggt agtcctcagt atgatggagc agtggttcgc cctgaacaag
     7021 gcctgatggc tctccgtaag gaactcaatc tttacgctaa tattcgtcct gtaaaaatct
     7081 ttgacagtct caagcatttg tcaccactca aactggaacg aattgctggt gtagactttg
     7141 tcgtggtgcg tgaattgaca ggcgggattt actttggaga tcatattctt gaagagcgca
     7201 atgcgcgtga tatcaacgac tatagctatg aggaagtgga gcggattatt cgcaaagcct
     7261 ttgaaattgc aagaaatcgc agaaaaatcc ttactagtat cgataagcaa aatgttctag
     7321 cgacctcaaa actctggcgg aaagtagctg aggaagtcgc acaggatttc ccagatgtaa
     7381 ccttggaaca ccagctggta gactcagctg ctatgcttat gattaccaat cctgctaagt
     7441 ttgatgttat tgtaacggag aatctttttg gagatatttt atctgatgaa tcaagcgtct
     7501 tatctggtac acttggggtt atgccatcag ccagtcattc cgaaaatgga ccaagtctct
     7561 atgaacctat tcacggttca gcacctgata ttgcaggtca aggaattgcc aatcctattt
     7621 ccatgatttt atcagtttcc atgatgttga gagatagttt cggacgttat gaggatgcag
     7681 agcgtatcaa acgtgctgtt gagacaagtc tggcggcagg aattttaacg agagatatag
     7741 gaggtcaggc ttcaacaaag gaaatgacgg aagctattat tgcaaggtta tgaagttaga
     7801 cgaaaaaatt actctagtcc ttttgatttg gaatgtcatc attttcttga tttatggcat
     7861 tgacaaattc aaggcaagaa gaagagcttg gcgcatccaa gagaaaatct tactcatttt
     7921 agcccttact tgtggtggtt ttggggcctg gttagctgga atcacttttc accacaagac
     7981 tcgaaaatgg tactttaaaa cagtttggtt tcttgggatg gtgaccacac tagtagcctt
     8041 atattttatt tggaggtaat ggatggcagg gtcttcgagg gaacacgctg cttgggctct
     8101 agcggactat ggttttaagg tcgtgattgc aggatctttc ggtgacattc attacaataa
     8161 tgaactcaat aatggcctgt tgccaatcgt tcagcctaga gaggttagag agaaactagc
     8221 ccagctaaaa ccaaccgacc aggtaactgt ggacttggaa caacaaaaaa tcatctcacc
     8281 agttgaagaa ttcaccttcg agatagatag cgagtggaaa cataaactcc taaatagttt
     8341 ggatgatatc ggtattacct tgcagtatga agagttgatt gctgcttatg aaaaacaacg
     8401 accagcctac tggcaggatt agaaaaaata gaaaaggaaa tatagtaaac tgaaataaga
     8461 tgtaaacaaa tgaattagag cttaacatcc atttccagca attttttaga aactacagtg
     8521 gactattctg gattcaacac attataaaat tatgacaaaa cacattcaca agaaggctac
     8581 gacattttaa aaggtgaggg cggatgtatc gtttgcccta ctaaagttgg ttacattatc
     8641 atgaccagca acaaggcagg acttgagcgt aagttcgcag ccaaagaacg taagcgtaac
     8701 aaaccaggtg ttgttctctg cggtagcatg gatgaacttc gcgctttagc gcaactcaac
     8761 ccagaaattg aagcattcta ctaaaaacat tggatgaaga tattcttctt ggttgtatcc
     8821 ttccttggaa accagaagcc tttgaaaaac tcaaagcata cggggatggc cgtgaagaac
     8881 ttatgactga tgtacgtggt actagctgtt ttgttatcaa gtttggaaaa gcaggtgaac
     8941 aattggctgc caagctttgg gaagaaggta aaatggtcta cgcctcatat gcttcaatga
     9001 caaaacgatt gaaactcgct atgagagcaa ggtgtaatgg tgtctatggt cgataaggac
     9061 ggcaaactca tcccagaaca aggaggagca cgttcaactt caccagcacc agttgtgatc
     9121 cgtaaagggc ttgacattga taaaatcatg atgcacctgt cagatacttt taactcatgg
     9181 gactaccgtc aggttgagta ttattaagat aaaaaaagaa gtctagtgtt atgagatatt
     9241 aaagctccta acactggact ttttgtttag aatttctttt ctttttctat aggatatggt
     9301 attctatgta gaaaatatat gttaataagt aatgccaata tttaaacatc attagtaaaa
     9361 ggagttagat tgatgaataa aagaaaagtt agtttagaag atttttataa atggtatagt
     9421 ctaaataaag aagagttatt aaataaggca actgttggtg aaaagtttaa tgataaatta
     9481 aaagaagagt ttctccagga atggcctttg gataggattt taacaatgtc aatcgatgaa
     9541 tatgtaatag gaaagggaca gcaaaataag tctttatgct acgctcttga gaagggaaaa
     9601 tacaaaaatc tatttcttgg aatttctggt ggctcagctt caaaatttgg tatttattgg
     9661 aataaaaaaa caaacaaata taaagatcaa gctaataatg agatttcaga gttggatcag
     9721 cgattttcaa aattaaaatc agatttgtat gaaattatca aagaaggtat tcgttttaac
     9781 tttgaaaatc ctatttttga tatgaaaaga tcaacaaatg aatttattgg tcgttctgct
     9841 atggtgacaa aattactttg tatctatact gagggagatc ctttctttgg tgtaaatatt
     9901 aatagtcaga aagaattttg gaaccacttt gtttctcaga caaatcaagg tggaccttat
     9961 ctgcaaaatc ataaaataat tgaactagtg tccaaaactt atcctgagtt ggagccatcg
    10021 aaattaggaa ctatgctttt tgagtattct aagcttttta tggaaaataa ggaagacaat
    10081 agtacaatgg attcatcaaa caattttcgt catcaattaa ctcaatctct attaaagtct
    10141 ccaaacctta tccttcgtgg tgctcctggc acaggaaaga cttatcttgc taaagaaatt
    10201 gccaaagaat taacggatgg caacgaagac caaatcggat ttgtacaatt tcacccatcc
    10261 tatgattata cggattttgt agaaggttta agaccagtat caaatgggga tggagctatt
    10321 gagtttaggc tacaggacgg tatttttaaa gatttttgtc agaaagcaaa agaaacccaa
    10381 ttgattggag gacaagataa ttttgatgag gcttgggatt cttacttaga atatataaat
    10441 gttgctgaag aaaaagaata tataacaaaa acatcttact tatctgttaa tagtagacaa
    10501 aatttgtcag taaattatga tagtggtgtt ccaggatggt cactacctag caaatatgtt
    10561 tacgagttgt ataaagataa aaattataat aagcaagaat actacaaaag tggtggaaaa
    10621 actgtcctag aaacattgag aaagagattt ggtttgaaag actatgtttc cccaacagaa
    10681 attgatactg ataagaaatt cgtcttcatc atcgatgaga tcaatcgtgg ggagatttct
    10741 aagatttttg gcgaactctt tttttctatc gaccctggct atcgtggtga aaaaggaagt
    10801 gtttctaccc aatatgcaaa tctacacgaa actgatgaaa agttctatat ccccgaaaat
    10861 gtttacatca tcggaactat gaatgatatt gatcgttcag tggatacctt tgattttgct
    10921 atgcgtcgtc gttttcgttt tgttgaagtt actgccgagg gtcaagttgg catgttggat
    10981 aaagagttga atatccatgc agaagaagca aaaattcgtc taagaaactt gaacgctgct
    11041 atcgaaaatg ttcaggaatt aaacagtcat tatcatattg gaccaagtta ttttcttaag
    11101 ttgaaggatg tagattttga ctatgaatta ctctggtctg attatattaa gcctctccta
    11161 gaagactact tgcgaggttc ttatgatgag gttgaaactt tggaaacttt gaaaaaagca
    11221 tttgaactga caaataatga gcaaaaagat cagacagtag ctgatgacaa tgaaggtgat
    11281 gaaaacgatg atgcggatta ctgataatca acacaagatt attaaagaaa aatttgttga
    11341 agaatatcct aaactaagca atcttctttt agacagaacc ttggaaagtc tatcccaaga
    11401 tgaacgtatt ttcatttttc caaatgattt gactcatact cctgatttgg ataaggacca
    11461 aaagattttt gaaacagtca atcagaaaat caagacaggg aacgtgattg gttttcttgg
    11521 atatggtcag gaaagattaa cgatttcctc acgattttct gatgagagta atgaccattt
    11581 tttgcattat ctcttaaaca aggttcttca tatcaatctc actagtttag atgttgcttt
    11641 gtctcgtgaa gagaggcttt atcaactttt gatgtatctc ttccccaagt atctacaagc
    11701 tgctattcga aaaggtcttt ataaggaata tcatcgattt tctcataacg acagtcatgt
    11761 taagggagtg attgatgtaa gaagccatct caagaaaaat cttcctttca cgggaaatat
    11821 tgcctacgca acgagagagt tcacctatga taatcccctc atgcagttgg tccgtcacac
    11881 tattgaatac attaagaatc agaaaagcat tggtcaaggg gtactagata atctctcaac
    11941 tagtcgtgaa aacgtatctg aaatcgtgcg tgtaacgccc tcttataaac tagctgatcg
    12001 tgctaagatt actcggagaa atcaatctaa acctatacgt catgcatact ttcacgagta
    12061 cagaaactta caagaacttt gtctgatgat cctaaaccaa gaaaagcacg gtttagggta
    12121 tcaagatcaa aaaatctatg gtattctctt tgatgttgcc tggctttggg aagagtatgt
    12181 ttacaccttg ttgccaaaag gttttgtaca tcccagaaat aaggataaga cggatggaat
    12241 ttcagtattt tctgttggga aacgaaaagt atatccagat ttttatgaca gagaacgaaa
    12301 gattgttcta gatgcaaaat ataaaaaact ggaattgact gaaaaaggaa tcaaccgtga
    12361 ggacttattc cagctgattt cctattctta tattttaaaa gctgagaagg ctggacttgt
    12421 ttttccgagc aaggacaagg tcattgataa tgagatagga aacctagcag gttatggagc
    12481 tttgttgaag aaatggtcta tccaaattcc tcagaagtct tcatcttata gtgcattttg
    12541 taaaatgatg gaaaattcag aagaaaattt taaagcgatt attgatgaag aagtggggag
    12601 aaaataattt cccctctttc ttttattttt a
//
LOCUS       DAYYQN010000053        12573 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYYQN010000053 DAYYQN010000000
VERSION     DAYYQN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.054"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            19..411
                     /locus_tag="ACHBRQ_001595"
     CDS             19..411
                     /locus_tag="ACHBRQ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593229.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            433..1224
                     /locus_tag="ACHBRQ_001596"
     CDS             433..1224
                     /locus_tag="ACHBRQ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593230.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1217..1858
                     /locus_tag="ACHBRQ_001597"
     CDS             1217..1858
                     /locus_tag="ACHBRQ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593231.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            1862..2494
                     /locus_tag="ACHBRQ_001598"
     CDS             1862..2494
                     /locus_tag="ACHBRQ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9593232.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2522..2809
                     /locus_tag="ACHBRQ_001599"
     CDS             2522..2809
                     /locus_tag="ACHBRQ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593233.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            2955..5468
                     /gene="secA"
                     /locus_tag="ACHBRQ_001600"
     CDS             2955..5468
                     /gene="secA"
                     /locus_tag="ACHBRQ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9593234.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5549..6580
                     /locus_tag="ACHBRQ_001601"
     CDS             5549..6580
                     /locus_tag="ACHBRQ_001601"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9593235.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6582..7613
                     /locus_tag="ACHBRQ_001602"
     CDS             6582..7613
                     /locus_tag="ACHBRQ_001602"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9593236.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7658..8020
                     /gene="acpS"
                     /locus_tag="ACHBRQ_001603"
     CDS             7658..8020
                     /gene="acpS"
                     /locus_tag="ACHBRQ_001603"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9593237.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            8010..9113
                     /gene="alr"
                     /locus_tag="ACHBRQ_001604"
     CDS             8010..9113
                     /gene="alr"
                     /locus_tag="ACHBRQ_001604"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9593238.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFIEASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9132..11147
                     /gene="recG"
                     /locus_tag="ACHBRQ_001605"
     CDS             9132..11147
                     /gene="recG"
                     /locus_tag="ACHBRQ_001605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9593239.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11463..12443
                     /locus_tag="ACHBRQ_001606"
     CDS             11463..12443
                     /locus_tag="ACHBRQ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HFM9593240.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         3670 a         2355 c         2870 g         3678 t
ORIGIN      
        1 tgaaaggaaa cagaaaaaat gaaaattaag aaattattga aaatggttat tcctgtttta
       61 atgataagtg ctgttggaac tactttcgta gaagcaaatc agataggtgc ttttagtaat
      121 tttgttatta ctaccagtta taagagaaca ggttatttga ctaaagaaaa tgaaggtgcg
      181 gaatatatca tgaacttaaa tccttgtaga aatttacatc ccatgactgt taagcatcgt
      241 atagtaaatt ctaatggaga agctcgtagt ggagaatcgt taacaacctg tggtactcgt
      301 tcaactcatg gtaattgggc aacggttggc tatgtttatg cagcagatat ggcaagacaa
      361 aattggtggg atttatcagc tgccatttca ggtagttggt caccaaatta attcgtaaag
      421 taggtataat ttatgaagat tcctctctta acttttgcaa ggcataaatt tgtttatgtc
      481 ttgcttactt tgctttttct tgctttggtt tatcgtgatg ttttgatgac ttatttcttt
      541 tttgatattc atgcgcccga tctagctaaa ttcgatggac aagcaattaa aaatgactta
      601 ttaaaatcag cattagattt tcgtattctc cagttcaatc taggttttta tcaatcattt
      661 attattccaa tcatcattgt tttgctaggt tttcaatata ttgagctgaa aaataaagtt
      721 ttacgattga gtattggaag agaagtgagt tatcaagggt taaaaagaaa gttgactttg
      781 caagttgcaa gtatcccttg tttgatatat ttagtgactg tgctgataat tgcaattata
      841 acctatttct ttgggacttt ttctcctctt ggatggaatt ctctattttc tgatggaagt
      901 ggtttacaaa gactcctaga tggagagata aaaagctatt tgttctttac ttgtgtccta
      961 ctaatcggta ttttcatcaa tgcaatctat tttttacaaa tagttgatta tgtggggaat
     1021 gtgactcgtt cggcaatcac ctatttgatg tttctttggc ttggttctat gctgctctat
     1081 agtgccttgc cttactatat ggttcctatg acgagtttga tgcaagctag ctatggggat
     1141 gtaagtttga tgaaactctt tactccttat atcctttata ttgtccctta tatggtgctt
     1201 gaaaaatatg aagataatgt ttaagaattt taacaatatt tggctaaaga gaaagatcgt
     1261 tttactactt cgtatagttc tgatgatgat tttgataaac tatctattgt caacagcggt
     1321 tcaaaagcag gatgctgtta tctttttcaa gagagaattg atttcaatct tttcctataa
     1381 tgactattct gaagcgaatt tagaaatccc caaactcttg ttaaaccttt cgcttttcat
     1441 ggtaggatgg ctctctgtca ttttacttga aagtgatttg gcagaccatt accatcactt
     1501 gattcgctat caatcaagct cctttttcga ttatacaagg aaacgattgg ttgtcatttc
     1561 taaatttttt actcaagatt tacttgtctg gttccttggt ttacttccgc taggaattca
     1621 tttcaaaaca gtcgcacttt tctttttact tgctcagtta atgattttgt acttactact
     1681 gtcttatctg atagcactga ttagtgcggg cgctggtttt tccttttttc tctatttttt
     1741 agcatttgtg ggacaagaat ggatgatgga tcatattgta acagtgtatt tactactctt
     1801 aagcttatta gttatgttga ttgttagtcg cttggaagag aaatttaaga aaggataaac
     1861 gatgagactt gaaattataa atggacagaa aatttatggg aaaagaccta ttttaaatca
     1921 gttgaatttg gtgtttcaat caggaaaaat ttatggactt aaaggtgata atggatctgg
     1981 caagacggtt cttttaaaga tacttgctgg ttatattaag cttgacaaag gaaaagttct
     2041 tcaagacggg aaagtctacg ggataaaaaa tcattatatt caggatgcag gaattttaat
     2101 tgaaaaagtc gagtttttat ctcatttatc cctgagagaa aatttggaac tgttaaggta
     2161 tttttcatct aaagttacgg aaaaaagaat tgcctattgg attcaatact atgatttaca
     2221 ggaatttgaa gacattgaat accgtcattt atccttagga acaaagcaaa aaatggcctt
     2281 gattcaagcc tttatttcct ctccttctat actctttctc gatgaaccta tgaatgcttt
     2341 ggatgagaag agtgtgaggt taaccaaaca ggtcatttta tcttacctga aaaaagaaaa
     2401 tggtctggtt atcctgacat cgcacatatc ggaagatatt tcagaccttt gtacagatgt
     2461 attagttgtc gaaaatggac atatacaaat gtaaaggata tacaatccta ggagatggct
     2521 tatggcacat ctaaaatcat ttattacacg ctattccaag gtttatattg gtttagttct
     2581 gctgatctgg ctgtctttct tctttatccc ttgggataga gcgattctgg gggtaaggat
     2641 tgacatcttc atcatacaga aaatcttgct agcttttgga attctgtcca ttctcatggc
     2701 cttgctgtcc aagaaagtca gtctctttgt ttttggactg atttgctgtc tttctctttg
     2761 gattaactta tttatcacat ttgccatttt gccgattttt ggcaattaaa cagtcataaa
     2821 agtcggagag gttagcttga aaactaacct ctttttcctt ttcaaaatgg ggattcttcc
     2881 ttgaaaataa tcagtaattg tgctaaaatt aaaggaacat tctaaaatat tcggaattta
     2941 aagtaaggaa aaacatggct aatattttaa aaacaattat cgaaaatgat aaaggagaaa
     3001 tccgtcgtct ggaaaagatg gctgacaagg ttttcaaata cgaagaccaa atggctgctt
     3061 tgactgacga ccaactaaaa gcaaaaacag ttgaatttaa ggaacgttat caaaatggag
     3121 aatcactgga ttcattgctt tacgaagcat ttgcggttgt ccgtgaaggt gccaaacgtg
     3181 tcctaggtct cttcccttat aaggttcagg tcatgggggg gattgttctt caccatggtg
     3241 acgtgccaga gatgcgtaca ggggaaggga aaaccttgac tgcgaccatg ccggtatacc
     3301 tcaatgccct ttcaggtaaa ggggttcacg tagttacggt taatgaatac ctgtcagaac
     3361 gtgacgcgac tgagatgggt gaattgtact cttggcttgg tttgtcagta gggattaact
     3421 tggctaccaa atctccaatg gagaaaaaag aagcctatga gtgtgatatt acttactcaa
     3481 ctaactcaga aatcggattt gactaccttc gtgacaacat ggtcgttcgc gccgaaaaca
     3541 tggtacaacg tccgcttaac tatgccttgg tcgatgaggt tgactctatc ttgattgacg
     3601 aggctcgtac acctttgatt gtatcaggtg ccaatgcggt tgaaaccagt cagttgtatc
     3661 acatggcaga ccactatgta aaatctttga acaaagatga ctacatcatc gatgtgcagt
     3721 ctaagactat tggtttgtct gattcaggga ttgacagggc tgaaagctac ttcaaacttg
     3781 aaaacctcta tgacatcgaa aacgtggctt tgactcactt tatcgataac gcccttcgtg
     3841 ccaactacat catgcttctc gatattgact atgtggtgag cgaagagcaa gaaatcttga
     3901 ttgtcgacca atttacaggt cgtaccatgg aaggtcgtcg ttattctgat ggattgcacc
     3961 aagctattga agccaaagaa ggtgtgccaa tccaggatga aaccaagaca tctgcctcaa
     4021 tcacgtacca aaacctcttc cgtatgtaca agaaattgtc tggtatgacg ggtacaggta
     4081 agactgagga agaagaattc cgtgaaatct acaacattcg tgttattcca atcccaacaa
     4141 accgtcctgt tcaacgtatt gaccactcag accttcttta tgcaagtatc gaatctaagt
     4201 ttaaagcggt tgtcgaagac gttaaggctc gttaccaaaa gggtcaacct gtcttggttg
     4261 gtacagtagc ggttgaaact agtgactaca tttctaagaa attggttgca gctggtgttc
     4321 ctcacgaagt cttgaatgcc aaaaaccact atagagaagc ccaaatcatc atgaatgctg
     4381 gtcaacgtgg tgccgttacc atcgcaacca acatggcggg tcgtggtacc gacatcaagc
     4441 ttggtgaagg tgttcgtgaa cttggaggac tttgtgttat tggtacagaa cgtcatgaaa
     4501 gtcgtcgtat cgataaccag cttcgtggac gttcaggtcg tcaaggagat ccaggtgagt
     4561 cacaattcta cctatctctt gaagatgatt tgatgaaacg ttttggttct gaacgcttga
     4621 agggaatctt tgaacgcttg aacatgtctg aagaggccat tgagtctcgc atgttgacgc
     4681 gtcaggttga agcagctcag aaacgtgtcg aaggaaataa ctacgatacc cgtaaacaag
     4741 tccttcaata cgatgatgtc atgcgtgaac aacgtgagat tatctatgct caacgttacg
     4801 atgtcatcac tgcagatcgt gacttggcac ctgaaattca gtctatgatc aaacgcacga
     4861 ttgaacgtgt cgttgatggt catgcgcgtg ccaaacaaga tgaaaaacta gaggcaattt
     4921 tgaactttgc taagtacaac ttgcttcctg aagattctat tacgatggaa gacttgtcag
     4981 gcttgtctga taaggccatc aaggaagagc ttttccaacg ttccttgaag gtttacgata
     5041 gtcaggtttc aaaactacgc gatgaagaag cagttaaaga attccaaaaa gttttgattc
     5101 tacgagtggt ggataacaag tggacagatc atatcgatgc ccttgatcaa ttgcgtaacg
     5161 cggttggact tcgtggctat gctcagaaca accctgttgt tgagtatcag gcagaaggtt
     5221 tccgtatgtt taatgatatg attggttcga ttgagtttga tgtgacacgc ttgatgatga
     5281 aagcacaaat tcatgaacaa gaaagaccac aggcagaacg tcatatcagt acaacagcga
     5341 ctcgcaatat cgctgctcac caagcaagta tgccagaaga tttggatttg agccagattg
     5401 gacgcaatga actttgccca tgtggttctg gtaagaaatt taaaaactgt cacggtaaaa
     5461 gacaataaaa tgagatagtt tagaggcgga tatcttgtga aaagtaaatt tttactgggt
     5521 atccgtttgc tttataagga gatgagttat ggtatttaca gcaaaaagct ctaaaataaa
     5581 tatagaagaa gttcgtgcct tgtcaaaatt agaaggtcag gctttggaga ggaaatcaca
     5641 gagagatcaa gagctagaag ccattatacg tggagaagac cagcgaattc tcttggtaat
     5701 cgggccatgc tcatctgata acgaagaagc tgtccttgaa tacgctaagc gtttggcagt
     5761 cctacaagaa gaagtggcag atcgtatctt tatggttatg cgtgtttata ctgccaaacc
     5821 ccgtaccaac ggagatggct ataaaggctt gattcaccag cctaacgcga cagaagcgcc
     5881 tagtcttatc aatggaatca aagccgttcg ccatcttcac tatcgtgtca tcacagaaac
     5941 agggatgaca actgctgatg aaatgcttta tcctgaaaac cttccgcttg tagatgattt
     6001 gatttcttac atggcagttg gtgcccgttc agttgaagac cagcaacacc gctttgtggc
     6061 aagtggggca ggattttcta ctggttttaa aaatccaacc tctggaaatc tcaatgtcat
     6121 gtttaatggg atttatgctg ctcaaaacaa acaaagtttc cttttcttag gaaaagaagt
     6181 agaaacaact gggaacccgc tttcacacgc tattcttcgt ggtgctctta atgagtatgg
     6241 aaaaaatatt cccaactact attatgacaa tttaattgat accattgccc agtatgagaa
     6301 aatgggcttg gaaaatcctt ttatcatcat tgataccaat catgacaatt ctggtaagca
     6361 gtatattgaa cagatccgaa ttgtccgcca gaccttgatt aaccgtgctt ggaatgaaaa
     6421 aattaagcag ttcgttcgtg gttttatgat tgagtcttat ctagaagatg gtcgacaaaa
     6481 tgagccagaa gtatttggta agtctatcac agacccttgc ctgggttggg ataacacaga
     6541 agctcttgtc agagaaattt acaaaacgtt aggagaataa gatggcattt attgaaaaag
     6601 gtcaagaaat cgatatggaa gtcatcaagg ctgaaaccca attgtctgcg gaagccttga
     6661 gactcaagga aagccgtgac agggaattgg cagatattat ttcaggggaa gatgaccgta
     6721 ttctcttggt gattggtcct tgctcttctg ataatgaaga ggcggtcttg gaatatgctc
     6781 gccgtttatc cgccttgcaa aagaaggtag cggacaagat tttcatggtc atgcgcgtgt
     6841 atactgctaa gcctcgtacc aatggagacg gctataaagg attggttcac cagccagata
     6901 cttctaaggc tccaagcctg attaacggct tgcaggctgt gcgccagttg cactaccgcg
     6961 tgattacaga gactggtttg acaacggcag atgagatgct ttatccgtca aatctgatct
     7021 tggtggatga cttggtcagc taccatgccg ttggagctcg ttctgtggaa gaccaagagc
     7081 accgctttgt ggcttctggg attgatgcac cagtagggat gaaaaatcca acctcaggaa
     7141 atttgggtgt tatgtttaac gccatctatg ctgctcaaaa caagcaaacc ttcctttatc
     7201 atgggcagga agttgagaca tcaggtaatc ctttggccca tgttatcctc cgtggagcag
     7261 tcaacgagta tggcaattat atgccgaatt actactatga aaacctactc caagccattg
     7321 aacgctatga aaccatggga cttgaaaatc cttttatcct cattgacacc aaccatgata
     7381 actcaggcaa gcaatatatg gagcagattc gaattgttcg ccagaccttg cagaatcgtg
     7441 attggaatga gaaaattaaa aagacggttc gaggatttat gattgaatct tacctagcag
     7501 atggtcgtca aaaccaacca gagatctttg gttgctctat tactgaccct tgcctaggtt
     7561 gggaaaatac agaggccttg gtagaagaga tttatgttac cttgacaaaa taagtgaaaa
     7621 ggatggagtt ggggaatctc aactcctttt gatgagaatg atagttggac acggaattga
     7681 catcgaagaa ttggcttcga tagaaagcgc agttacacga catgaaggat ttgctaagcg
     7741 tgtactgacc gctcaggaaa tggagcgctt caccagtctc aaaggacgca ggcaaataga
     7801 atatttagct ggtcgctggt cggctaagga ggccttttcc aaggctatgg gaacgggcat
     7861 tagcaagctc ggttttcagg atttggaagt cttgaacaat gaacgtgggg cgccttattt
     7921 tagtcaggca ccattttcag gaaagatttg gctgtctatc agccacaccg atcagtttgt
     7981 gacagccagt gtcattttgg aggaaaatca tgaaagctag tccacataga ccaaccaagg
     8041 ctctgattca tctgggagct attcgacaaa atattcagca aatgggggct catatccctc
     8101 aaggaacgct caagttggct gtggtcaagg ccaatgctta tggtcatgga gctgttgccg
     8161 ttgccaaggc aattcaagat gatgttgatg gcttttgcgt ttccaatatc gatgaagcca
     8221 ttgaactcag acaagctgga ctcagcaagc caatcctcat tttaggagtt tctgaaatcg
     8281 aagctgttgc tctagctaaa gaatatgact tcaccttgac agtggctgga ctggagtgga
     8341 ttcaagcact cttagataag gaagtggacc taactggatt gacagtccac ctcaagattg
     8401 attcagggat gggacggatt ggttttatag aggcaagtga ggttgagcag gctcaagatt
     8461 tgctccaaca acacggtgtt cgtgttgaag gaatctttac ccactttgct actgctgatg
     8521 aggaatcaga tgactatttt aatgcccagt tagaacggtt taaaactatt ttagctagta
     8581 tgaaggaagt tccagagctg gttcatgcta gcaattctgc aacgactctt tggcatgtag
     8641 agactatttt caatgcggtt cgtatgggag atgccatgta tggtctcaat ccaagtggag
     8701 cggtcttgga tttgccttat gatttgatac cggccttgac cttggagtct gctctggttc
     8761 atgtcaagac agttccagct ggagcttgca tgggctatgg agcaacttat caagcggata
     8821 gcgagcaagt catcgcgacc gtgccaatcg ggtatgcaga tggatggaca agagacatgc
     8881 aaaatttctc tgtcttggta gatggccaag cttgcccaat tgttggcagg gtttcgatgg
     8941 accaaatcac tattcgattg cctaagcttt atccgctagg aaccaaggta accttgattg
     9001 gctccaatgg ggataaggaa atcactgcaa ctcaggtagc gacctaccgc gtaaccatta
     9061 actatgaggt ggtttgcctc ctcagcgacc gtattccgag agaatattat tagaaaagaa
     9121 aggagtggag catgaatcta catcaaccct tgcatgtctt gcctggtgtg ggaccaaagt
     9181 cagcagaaaa atacgccaaa ctaggaattg aaaacttgca agatctcttg ctctactttc
     9241 ctttccgtta tgaagacttc aaaaccaagc aggtgctgga gctggaagac ggtgagaagg
     9301 cagttctttc tggtcaggta gtgactcctg ctagtgtcca gtattatggt ttcaagcgca
     9361 atcgcctgcg ttttagtctc aagcagggag aggtcgtttt tgcggtgaat ttctttaacc
     9421 agccctatct ggctgataaa atagagttgg gagcaaccct tgctgtcttt ggaaaatggg
     9481 accgcgctaa ggctagtctg actgggatga aggttctggc tcaggtagaa gatgacctcc
     9541 agcctgttta tcgtctggct cagggaatca gtcaggccag tctggttaag gtcatcaaga
     9601 cggcttttga tcagggactg gacctcttga tagaagaaaa tctgccccag tctttactag
     9661 acaaatacaa actcatgtcc cgttgtcagg cagtccgagc tatgcatttt ccaaaggatt
     9721 tggcagaata caagcaggct cttcgccgta taaagtttga ggaactcttt tatttccaaa
     9781 tgcagctgca gacgctcaag tctgaaaata gaattcaggg aagtggtctg gttctggatt
     9841 ggtctcagga aaaagtgaca gcagttaaag caagtcttcc ttttgccctg acccaagctc
     9901 aggaaaagag tttgcaggaa attttaactg atatgaagtc cgaccaccac atgaatcgtc
     9961 tcctacaagg ggatgtgggg agtggaaaaa cggtagtcgc tggcttggcc atgtttgcgg
    10021 cagtgacagc aggttatcag gctgccctaa tggtaccaac agaaatcctc gcagagcaac
    10081 actttgagag tttacagaac ctttttccca atttgaaact ggctctcttg acaggttcct
    10141 tgaaagctgc agaaaagaga gaagtcttgg agaccattgc caagggtgag gctgatttga
    10201 ttataggaac tcacgctctg atacaagatg gggtggagta tgctcgtctt ggtttgatta
    10261 ttatcgatga gcagcaccgt tttggtgtag ggcaaaggcg tattttacgg gaaaaaggtg
    10321 acaatccaga tgtcctcatg atgacggcga ctcccattcc acggacgctt gccatcacag
    10381 cctttggaga tatggatgtt tccattatcg accagatgcc agcaggtcgg aagcctattg
    10441 tgacgcgctg gatcaaacat gagcaactac ctcaggtctt gacttggtta gagggggaaa
    10501 ttcaaaaagg ttcccaagcc tatgtcatct ctcctttgat tgaagaatca gaagctctag
    10561 atttgaaaaa tgccattgcc ttatcagaag agctgacggc tcattttgca ggcaaggcag
    10621 aggtggctct tctacatggt aggatgaaga gtgacgaaaa agaccagatc atgcaggatt
    10681 tcaaggagag aaagacggat attctggttt cgacgacggt tattgaggtt ggggtcaacg
    10741 ttcccaatgc gactgtcatg attatcatgg atgccgatcg cttcgggctc agccagcttc
    10801 accagcttag aggtcgtgtc ggtcgggggg acaagcagtc ctacgctgtt ctcgttgcca
    10861 atcccaagac ggattctggg aaagaccgca tgcgcatcat gacagaaacg accaatggat
    10921 ttgtccttgc ggaggaagat ttgaaaatgc gtggttctgg tgagattttt ggaaccagac
    10981 agtcaggact tccagagttc caagtggctg atattatcga agattttccg attttagaag
    11041 aagcaagaaa ggttgctagc tacattagtt ctatagaagc ttggcaagaa gatccagagt
    11101 ggcgcatgat tgcccttcat ctggaaaaga aagaacatct ggattaagct ttctctaagg
    11161 aaaacttaag ctagttttta ggtttggctc ttatactaaa gtcatcaaaa agaaacgagg
    11221 actctcatat gacagtaacg attaaagtaa attaccaaac cactttccaa aagaaggaag
    11281 caaaaaacta gtataaacag aagagagagc gaaatgctct ttttcgtttc taaaactact
    11341 ttcagcccat catcctaaaa gtaaagaatc taaattcact ttctatttat ccttctttct
    11401 tgcattgatt acatagatat gctacagttg tggtaacgat tacaaaataa aaggagcatg
    11461 ctatgaaaaa tccagctttg ctagaagaaa ttaagaccta tagaggaagg gatgaggttc
    11521 cggaagactt tgatgctttc tgggatgggg aagtaaaaaa tgtttccacg cttccatcct
    11581 accacttgga ggaaagagat ttccacattc ctcaagtcaa gtgctatgag ttaacatttg
    11641 aaggaagcaa ggaaggaaag gtctatgcac gcattgttct tccaaagagt gaggagaagg
    11701 ttccactaat attccatttt catggttata tgggacgtgg ctgggactgg gccgacatgc
    11761 tgggcttcac cgtagctggt tacggtgttg tttccatgga tgtgcggggc cagtcaggtt
    11821 actcacaaga cggcttgcgt tctcctttag gaaataccgt gaaggggcat attatccgtg
    11881 gtgctatgga aggtcgggac cacctctttt ataaggatgt ttatctggat atttaccagt
    11941 tggtcgaaat tgttgctagt ctgtctcagg ttgatgagaa gcgtctttct agctatggtg
    12001 cctcacaagg aggggctcta gctctagttg cagcagcgct caatcctcga attcagaaaa
    12061 cagttgccat ttatcccttc ttgtcagact tcagacgggt gattgagatt ggtaatacta
    12121 gcgaggctta cgacgaactt ttccgttatt tcaagtttca cgaccccttc catgaaacag
    12181 aggaggaaat catggcgacc cttgcctata tcgatgtcaa aaatcttgcc catcgtatcc
    12241 aaggtgaggt taagatgatt acgggcttgg acgacgatgt ttgctatccc attacccagt
    12301 ttgcgattta taatcgtctg acctgcgata aaacctatcg catcatgcct gagtatgctc
    12361 acgaagccat gaatgtattt gtcaatgacc aagtctacaa ctggctctgt ggaagtgaga
    12421 ttccttttaa atatctaaaa taaggagtcg actctaagca caaaatctta aaaattacaa
    12481 acacgcatag tatcagggga ttaaaaaaac ttgatactat gcgttttatc atggaaatat
    12541 agtaaaatga aacaagaata ggacaaaacg atc
//
LOCUS       DAYYQN010000054        12198 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYYQN010000054 DAYYQN010000000
VERSION     DAYYQN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.055"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(112..549)
                     /locus_tag="ACHBRQ_001607"
     CDS             complement(112..549)
                     /locus_tag="ACHBRQ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9593241.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="ACHBRQ_001608"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="ACHBRQ_001608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9593242.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEF
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKSYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3523..4405
                     /locus_tag="ACHBRQ_001609"
                     /pseudo
     CDS             3523..4405
                     /locus_tag="ACHBRQ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            4532..4813
                     /locus_tag="ACHBRQ_001610"
                     /pseudo
     CDS             4532..4813
                     /locus_tag="ACHBRQ_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(4947..5915)
                     /locus_tag="ACHBRQ_001611"
     CDS             complement(4947..5915)
                     /locus_tag="ACHBRQ_001611"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9593243.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6060..6843)
                     /locus_tag="ACHBRQ_001612"
                     /pseudo
     CDS             complement(6060..6843)
                     /locus_tag="ACHBRQ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
     gene            complement(6868..7365)
                     /locus_tag="ACHBRQ_001613"
     CDS             complement(6868..7365)
                     /locus_tag="ACHBRQ_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFM9593244.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(7438..8799)
                     /gene="radA"
                     /locus_tag="ACHBRQ_001614"
     CDS             complement(7438..8799)
                     /gene="radA"
                     /locus_tag="ACHBRQ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9593245.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(8813..9328)
                     /locus_tag="ACHBRQ_001615"
     CDS             complement(8813..9328)
                     /locus_tag="ACHBRQ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9593246.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(9330..9773)
                     /locus_tag="ACHBRQ_001616"
     CDS             complement(9330..9773)
                     /locus_tag="ACHBRQ_001616"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9593247.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            10078..10227
                     /locus_tag="ACHBRQ_001617"
     CDS             10078..10227
                     /locus_tag="ACHBRQ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593248.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            10369..10548
                     /locus_tag="ACHBRQ_001618"
     CDS             10369..10548
                     /locus_tag="ACHBRQ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593249.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(10768..11133)
                     /locus_tag="ACHBRQ_001619"
                     /pseudo
     CDS             complement(10768..11133)
                     /locus_tag="ACHBRQ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
     gene            11153..11320
                     /locus_tag="ACHBRQ_001620"
     CDS             11153..11320
                     /locus_tag="ACHBRQ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM9593250.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(11475..11678)
                     /locus_tag="ACHBRQ_001621"
     CDS             complement(11475..11678)
                     /locus_tag="ACHBRQ_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593251.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(11701..11892)
                     /locus_tag="ACHBRQ_001622"
     CDS             complement(11701..11892)
                     /locus_tag="ACHBRQ_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9593252.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
BASE COUNT         3752 a         2866 c         2174 g         3406 t
ORIGIN      
        1 gtatctacaa cctcaaaaca gtgttttgag caacccgcag ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt tcgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag ccttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gttactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaatcctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttgg tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agctcttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac ggtcgtttgc agtcacatca tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaataat cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcaca tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaaaacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccggccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaaactca ttttcagcct gcatctcaag caaggtctct ttcttgacca taatcccagc
     1801 aatttccatc ttggcaagga caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatat caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 gtaattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaact ctcagacaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga agcccatctc atcatagaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcattg atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccagcgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagca agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactgctaaa atatttctat aaattaattt gactttcctg atagagttat
     3481 tcacatctta tttcaactca ctatagaagg aggaatagga ggattctcag acatccgggc
     3541 atcagcccaa ctaatgattt gattgctaag aaaatattca gcaatccaga aatcacttgt
     3601 caatttattc gcgatatgct ggacttgcca gcaaaaaatg tgaccatttt ggagggaagc
     3661 gatattcacg tattactctc catgccttac tcggtgcagg atttttatac cagtatagac
     3721 atcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtggacttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagagctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttgaaca
     4261 aggactagag cgtgggaaag ttgaaggaag ggaagaaagg aaactttttg ccttcctaga
     4321 catagtacgc caaggtcttc tgacttctga ggttgccagc cagcaattag gtatgtcagt
     4381 atctgaattt gaggcactgt tgtaaaatgg ctccataata tccatagtgg gtaaatcccc
     4441 tatggatatt atggagccta ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa
     4501 agcgacaaaa caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt
     4561 actagacatt aaagacccta atgtccagat tttaaacatc atcaataagg atacacacaa
     4621 ggaaatcatc gccaaactgg actacgacgc cccatcttgc cctgagtgcg gaaaccaatt
     4681 gaagaaatat gactttcaaa aaccttctaa aattccttat cttgaaacga ctggtatgcc
     4741 tactagaatt ctccttagaa agcgtcgatt caagtgttat cactgttcaa aaatgatggt
     4801 cgctgaaact tctgatgacg tacagtcata tttcttctct ttttattata tcacagtttt
     4861 aaatctagct ttactagatt caccgctact atctatttat tcggaaaaaa gacgaaaaaa
     4921 cctgagaatc atctcaggct tggtcattaa atttttttct caatatcgaa aagtggagaa
     4981 agtggtcgtt tttcatgaat acgtacgata gcatccccta ggagatgagc gattgaaatc
     5041 tgctcaatct tatcaatcaa acgctcttct ggaagataga tggtatccaa aacaaccaat
     5101 ttcttaatag ctgatttttg gatattgtcc gtagcaggac cagaaagaac tgggtgcgta
     5161 cagcttgcat agactgcaac agcaccagct tccgcaagag catctgccgc atgacaaatc
     5221 gttccagcgg tatcaatcat atcatcaatc aagatacaag tcttgccttc aaccttaccg
     5281 atgatgttca taacttcact agtattcatc ttatcaacgc tacgacgttt atcaataata
     5341 gcgatagatg ttttcaaaaa ttctgccaac ttacgagcac gagtcacccc tccatggtcc
     5401 gggctgacaa ccacatagtc agaaccaacc ataccacgcc gctcaaaata atctgcaatt
     5461 agaggagcac ccatcaaatg atccacagga atatcaaaga atccttgaat ttgcgcagca
     5521 tgcaagtcga tggtcaataa acgatccact ccagctactt caagcatatt tgcgacaagt
     5581 tttgaagtga ttggctcacg cgctctcgcc tttctatcct gacgtgcata cccatagtaa
     5641 ggcatgacaa cattgacaga ctctgcactc gcacgcttca aagcatctac cataatcaaa
     5701 atttcaagca gattgtcatt tacaggcgaa ctagttgatt gtaagataaa gacgtgtttc
     5761 ccacggattg attcttcaat gttgacctga atctctccat ctgaaaattg gcgaacactt
     5821 gatttcccca actctatccc aatctcctgc gccacacgtt ctgccaattc tttattagaa
     5881 gaaagggcaa acagctttaa atcagaaaaa gacatgattt cctccggtat atatgtatcg
     5941 cttgtgcttt tcacaagatt ttccatctac cattgtagcg ctttttgcac tatttttcaa
     6001 tcaaaaataa aagaagggca ccatatttgt acccttgcat cattcttttg aaaaatattc
     6061 taggtcatca actcattgtg tttctcaaca aagcaataag catgataata accatagaga
     6121 gcaatagccg taaccactgg aatcgctaaa ggcaactctg tttccaactc cacaaaagga
     6181 gagttaaaca ataagaccct gtttcttgcc aaccttctgt cctttatagg ctctgtaaag
     6241 caagtaaaca cctactacag ctagacctga aaaagtccag tgagaggcaa ttcctgagat
     6301 gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat ccgtacgaat
     6361 ataaccaata tcctcaatca tttggaatcc caaaccggag gcaattccaa gtaaaaacaa
     6421 aaattttaat tttcgcacag gaatcaaagc caaaacaaaa acaagtggca ataatttcaa
     6481 gggttcttct accaaaggag ccacaatagc actttcaaag gcatttaaaa atggactatc
     6541 tgggaaaaga acccccagta aatcatgtat ataagtatta gcaaaactag acaaccagcc
     6601 tgaaaggaac atccctccca ataaagacag aatcaaaacc ttctttggca attcccattt
     6661 tttcccaata cggaagagaa aataaagagc cggaatcatg taaaagagag ctagaaagat
     6721 agaaactccc attagtccat attccgcacc tgacctcgaa ccgtccgtat agtagatggt
     6781 ttcatactgt aaaccaatac atagcaataa aataaaaata aataaaatac tgcttttctt
     6841 catacacttt ctttctaaat gaagtattta taattctacg actgtcatac ttcctgtatc
     6901 aacattgtaa atagcaccag agataatgac atcgtctggt attaggggag actcgataag
     6961 cagttgcata tcctcgcgta cactctcttc tatatcttgg aagggcaaga agtcctggtc
     7021 tgacacatct acacctaatt cctcttttaa atactcctga aaaggttcat tttcaaaggt
     7081 ctgagcacca cagtctgtat ggtgcaatac cacaatctct cttgtcccca tttgttgctg
     7141 ggaaataact agcgaacgaa tcatgtcttc agtcactcga ccacctgcat tccgcaagat
     7201 atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg tgcaaacgtg agtccataca
     7261 ggtcacaatg gctactctgg ttttaggttt aagtggcaga tttaactgcc catgtagggc
     7321 aacataagcc tgattggctt gcataaactg ttcaaaatac gacacgattc cctccttgaa
     7381 aatttgatag tcaaatattt ctcctatctt atcattttta agagaatttg tcacggatta
     7441 tgcaaagacc tttttcaaga cttcctgaat cgttgtcacg ccaatgacct gaatttcctt
     7501 aggcagagtg attcctgtca aggaattctg aggtacataa atcttagtaa agcccagttt
     7561 agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc cgaatctctc ctgtcaagcc
     7621 cagttctccg acaaaacatt cctgaggatt agttggcttg tctttgtagc tcgaagcaat
     7681 agcaactgca acagccaagt caatggcagg ttcatccaat ttaacaccac cagcagattt
     7741 gagataggca tcctgatttt gcaagagaag ccctgcccgt ttttccaaaa cagccataat
     7801 caagctagca cggttaaaat caagtcctgt cgtagtacgc ttggcatttc caaacatggt
     7861 cggtgttacc aaagcctgaa cctccgccaa aatcggacgc gtcccttcca tggttacaac
     7921 gatggaggaa ccagtcgccc catccaaacg ctcttctagg aaaacttgac tcggattgag
     7981 tacctcaacc aagccgcccg actgcatctc aaaaatccca atctcattag tggaaccaaa
     8041 acgatttttg accgctctca aaatacgaaa ggtgtggtga cgctcccctt caaagtaaag
     8101 caccgtatcc accatatgct ccaacatacg aggcccagcc aaggttcctt ctttggtcac
     8161 atgacctacg ataaagatgg caatgttatt ggtcttggcc aactgcatga gttcagcggt
     8221 cacttcacgt acctgagaaa cagacccctg cacccctgaa atctcaggag acatgatggt
     8281 ctggatggaa tcaataatga gaaagtctgg ctggatacgc tccacttctg cacgaacact
     8341 ctgcatattg gtctctgcat agagataaaa ctcactatca atatcaccta agcgctctgc
     8401 acgtagttta atctgctggg cagactcctc cccactgaca tagagaactg tccccacttg
     8461 ggacaactgg gttgagactt gtaggagaag agttgatttc ccaatcccag gatccccacc
     8521 gatgaggacg agacttcctg gtaccactcc gcctccaagc acacggttga attcctccat
     8581 ctccgtcttg gttcgattga cattgatgga agtcacctca gctagtttca tgggcttggt
     8641 tttctcacct gtcaaggaca cacgcgcatt cttgacctcg gcaacctcaa cctcttccac
     8701 aaaagaagac caagacccac agttggggca acgtcccaga tatttagggg aattataccc
     8761 acaattttga catacaaatg tcgctttttt ctttgcgatg acaaacctct ttctatatct
     8821 ctaactcaca ctcaatcact tggcaaaaat caatcttctc atttggcaca aactggcgca
     8881 tgagcattcg atgagcaaca actaccacag tctgatgttc tcgatactta gacatacatt
     8941 ctagaaaccg agacttcatt tccgtagctg tctcatattg aataggacta ttaggaagca
     9001 actccccctt gttttctaaa aacagtcttc tagctgtttc aaagttttct attcctgttt
     9061 tatagacctg ccattcatgt aataaaggct ctactcttaa aggaagaccc gtagcacaga
     9121 ccacatacga agccgtttct aaagctcttg tgactgcaga agatacgatt atttcagctg
     9181 acgagagtaa aggatttttg ctcaatttct ggacttgctg ccgtcccatc tcagacaagg
     9241 gtgccaaatc tatcccaaat cctatataag aacgctcctc taactcacgg taatctggct
     9301 ccccatgacg tacaaagata atcttcattc tagtgccctg tcgatccaaa tccaccagtt
     9361 cgaacgccat cagctgcatc tccatctgca attaagaaag tagcaaaaac agcctggaca
     9421 atacgttccc caacttcaag aacaacctct tgatctgtga tattcttcat ctgcgcaaaa
     9481 atatgccctt catttccagg atttccataa taatccccat caatgacccc aactgagtta
     9541 attaaaacca agcccttctt acgaggattt gaagaacgat catagaggta gagaacctca
     9601 gtcggctgca tataagcctt aacccctgtc ggaaccaaga caatctctcc tggcgcaaca
     9661 actgtacgca cagcaacctt taagtcgtaa ccagccgcat gcgctgtctc acgcttgggc
     9721 aataaatttt catctgtaaa actcgaaacc aattcaaaac cacgaatttt cataattttc
     9781 tcttttctat tatcatttat tctagattat tctatcttat ttattcggaa aaagcacgaa
     9841 aaaaagagca cacaacaatt ataggcgata cgataattta cactgtttca caatacgttg
     9901 aaatttagag ctttaaagcg agggcaaagt tgatttttta catcgtttta cagaggttta
     9961 cgacactttt gccccttttt tgcccctttg tttttcaaaa aaacttcatc aaatcgcttg
    10021 actttcacgg tttaccgtga tataatataa tcaagataag gaaaggaggt gaggaagttg
    10081 aacaaagaag attggcttag gttactggaa aaggcgatag ataatatccc tgaaacggta
    10141 actgctatag caagtctggt gactgcaata acggtcgcaa ggcaaaacaa aaagcgtaaa
    10201 ccgaaatccc gtcaaagaaa aaggtgaacg ctaagaggtt ggggcgaaag cccctcacac
    10261 ctctatttta tcaaatgaaa agaggaaaag caatggttag tgcaatagct atttttataa
    10321 ttgcggtcaa tgtatatatt tatctaaaaa ataaaaagga caaataagat gagagaagtt
    10381 attcaagaat tgttagacag ttcgatgtct acatctgcta tttcccaagg tgctggagtt
    10441 ccatggacta ctgtttctga cctcagaaaa ggaaaaacaa gcatggacaa aatggcactt
    10501 ctcacagcag aaaaacttta tgaatttgct acaactgata agcagtgatt tcggtcactg
    10561 tttttatttt tgacaaaaca ccgtttttga caataatcaa aaaataaaaa ggagctatta
    10621 ttaacaaaat ggcgtttttg gcaataatac cctgccattc ctcctcccta ttcttcaaga
    10681 aaacgctttt ttgaagaatc agagcaaata aaaaaccgca agcctgagcc tgcggtgaaa
    10741 gaacaattta gaaagtttcc tttctattta tttaactgta atcaagccat ctggctctac
    10801 tgtgaactct ggcttgtctg ccagtgttcc gtctggtttg aggtagtacc agcctgttcc
    10861 gtccgctgac tggataaagg catttgatac catggcgcct tctttagcgt ctaagtagta
    10921 ccaagtgtcc ttgtacttga cccagcctgt cttcatggca ccttctacat caaaatagta
    10981 ccacttgtca gcgattttct tccagcctgt ggccatttcg cctgattggt caaagtagta
    11041 ccaattaccg tctgtgtgct tcttccagcg gtctgaaagc atatagcctg agccatcgaa
    11101 ataataccag ataccgttga ttttctcaaa cttatagaaa ggaggagata gtatggcaaa
    11161 tactccaata aaacctggaa cagataatca gaaacctggt cgctatgtag aggtaggacc
    11221 tcgtggcgga aaagttacta atggtcatac cgcaactatc ggaaaaggtg atcggctccc
    11281 tccgacatct gctaaaggca acggttggaa aaaagtctaa tcttcgtttg cgtacaatcg
    11341 ttcaatggtt gtacgctttt tccatacaca aaagcacatt cccaaaaaat caatttgaat
    11401 ccatgcttcg gcgtaatctt tcccattgct ggcatagtga gttatataat ggtgaatcat
    11461 tttattcctt cctcctattg agttacttga gaagtatcat gaatggcttc atagctaaga
    11521 cgcttaaatt cttctgagtc tatctgaaaa ttgataggct ttttttgtaa acactcaaga
    11581 aaactagtgt ttcttaaaag tttttcaact aactcagggt ctgcctttac aaaggtggac
    11641 tctttttgtc cactatacgg atatcgtttt ggtctcattt tcctactcct caaatctttc
    11701 ctactcaatc ccataatctt caataacctg aagaatgaaa ctgttcgctc gtggaccttt
    11761 agtcgttcca cttagaatgt ttgtcacttc ctgtcgttta aagccgtaag caaccgctag
    11821 agttgctttt ttaatgcctt tctctttcaa gaaagcaata actctttcgc gaccgtttgc
    11881 gatatctggc atattttctc ctttcttttt ctttcttctt tttctgctat aatataagca
    11941 gaaaggagtt aaccttatga cttttaaaga atatttactc aaagcaagca aacgcgacat
    12001 ctacgatgat ggtaaagatt ttgagaaatc aatttcagat tctcactaat cgagcgtgac
    12061 ttttttgata tggcaatctc agaaatttac caccagcgct gactacctta atgacttttt
    12121 caaggtggtc tttttcttgt tctaaatttt taagaatact taccatcagc acgttcctcc
    12181 tctttactta tttgtaaa
//
LOCUS       DAYYQN010000055        12183 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYYQN010000055 DAYYQN010000000
VERSION     DAYYQN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.056"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(1..1203)
                     /gene="rpsA"
                     /locus_tag="ACHBRQ_001623"
     CDS             complement(1..1203)
                     /gene="rpsA"
                     /locus_tag="ACHBRQ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9593253.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1365..1436)
                     /locus_tag="ACHBRQ_001624"
     tRNA            complement(1365..1436)
                     /locus_tag="ACHBRQ_001624"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1402..1404),aa:Gln,seq:ttg)
     gene            complement(1446..1526)
                     /locus_tag="ACHBRQ_001625"
     tRNA            complement(1446..1526)
                     /locus_tag="ACHBRQ_001625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1490..1492),aa:Tyr,seq:gta)
     gene            complement(1576..1806)
                     /locus_tag="ACHBRQ_001626"
     CDS             complement(1576..1806)
                     /locus_tag="ACHBRQ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9593254.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(1974..2618)
                     /gene="pcp"
                     /locus_tag="ACHBRQ_001627"
     CDS             complement(1974..2618)
                     /gene="pcp"
                     /locus_tag="ACHBRQ_001627"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9593255.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2633..3556)
                     /locus_tag="ACHBRQ_001628"
     CDS             complement(2633..3556)
                     /locus_tag="ACHBRQ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HFM9593256.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3553..4242)
                     /locus_tag="ACHBRQ_001629"
     CDS             complement(3553..4242)
                     /locus_tag="ACHBRQ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HFM9593257.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4307..5011)
                     /locus_tag="ACHBRQ_001630"
     CDS             complement(4307..5011)
                     /locus_tag="ACHBRQ_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9593258.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(5067..6089)
                     /locus_tag="ACHBRQ_001631"
     CDS             complement(5067..6089)
                     /locus_tag="ACHBRQ_001631"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9593259.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6221..8692)
                     /gene="parC"
                     /locus_tag="ACHBRQ_001632"
     CDS             complement(6221..8692)
                     /gene="parC"
                     /locus_tag="ACHBRQ_001632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9593260.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQIGIVPILSSYIAHRREVILARSRFDKEKSEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(8893..9097)
                     /locus_tag="ACHBRQ_001633"
                     /pseudo
     CDS             complement(8893..9097)
                     /locus_tag="ACHBRQ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(9112..11055)
                     /gene="parE"
                     /locus_tag="ACHBRQ_001634"
     CDS             complement(9112..11055)
                     /gene="parE"
                     /locus_tag="ACHBRQ_001634"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9593261.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLATVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11191..11832
                     /gene="plsY"
                     /locus_tag="ACHBRQ_001635"
     CDS             11191..11832
                     /gene="plsY"
                     /locus_tag="ACHBRQ_001635"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9593262.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(11875..>12183)
                     /locus_tag="ACHBRQ_001636"
     CDS             complement(11875..>12183)
                     /locus_tag="ACHBRQ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9593263.1"
                     /translation="ENTILHSDQGWQYQHDSYHRFLESKGIQASISRKGNSPDNSMME
                     SFFGILKSEMFYGYEKSFQSLKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG
                     "
BASE COUNT         3495 a         2767 c         2145 g         3776 t
ORIGIN      
        1 ttaaagttcg atatcaccaa acaaatcagc cattgaaaat cctgtttgtg tttctggaag
       61 ttcgaaatca cgcttttctt gacgtcttgg acgacgtgga cgagcagcac gtttttcttc
      121 tttttgtcct tcttcttggg ctggacgttc ttcaagagct ttaatagaaa gtgacacgcg
      181 ttctgcatct gcgttaactt caagaacttt aacttgaact tcttgaccaa ctttaagagc
      241 ttcttttgga ttttcaatcc gtttgtgtga aatttgtgat acgtgaacaa gtccatcgat
      301 acctggcaat acttcaacaa atgcaccgaa gtcagtcaaa cgtttaactg ttccttctac
      361 tacatcacct ttagccaatt tttgctcaac gccatcccat ggtcctggta ctgttgcttt
      421 aagtgaaagt gatacacgtc cttcttcttc gttaagatca aggattttca cttcaatttc
      481 ttcaccaaca gttacaactg atttgggtga tacgttacgt tcatgtgaca attcagtcaa
      541 gtgaaccaat ccgtcaacac caccaaggtc gatgaaagcg ccgaagcttg tgatacgagc
      601 aactttacca gttacaacat caccaacagc caatttaccg aatacttcag cgcgagctgc
      661 tgcagtagct gcttcaacaa cttcacgacg tgaaaggatg aagcggtttt ctttagcgtt
      721 aacttctttg attttagtat caaattcttg acctacaaaa cgctcagcgt tacgtacgaa
      781 acgagtatcc aacattgaag ctgggataaa tccacgaaca ccttcaaatt ctactgaaag
      841 tccaccttta acggcacgcg ttcctttaac agtaacaact tcttcttcgc gaccaacaag
      901 tttgtcccat gctttgcgag cttcaaggcg ttttttagat acaaggtatg taactgtatc
      961 agtatcttta ccaactactt gacgaagtac aagaacatcc aatacttctc ctactttaac
     1021 aaagtcattg atatctgcat cacgatcgtt tgtcaattcg cgaagagtca agacaccttc
     1081 aacaccagtt ccagagattg caacgttagc ttgagtcgca tcaactgtca atacttcagc
     1141 actaacaaca tcaccagtct caacttgact aacgctattt agcaaatctt caaattcgtt
     1201 catctaaaaa atcctccaac aatcaagttt ttcttaaact tgacatactt ataatttttt
     1261 tcctaagcac ccgcaaggac atgttcatat cattcacgtc tttcaattat aaaaataaaa
     1321 taccctaaga tattttgctt tttatcttga ttattaccta agaactgggg tagctggatt
     1381 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccattaa
     1441 tgaaatggag agagagggat tcgaaccccc gaacccgaag gagcggattt acagtccgcc
     1501 gcgtttagcc tcttcgctat ctctcctaca atcaacatgg actattatat catgaaaatt
     1561 tcaaaaaaac aagacttatt ttgctaaatt ataattttca atcaaaattt ccacagcttt
     1621 ttccaatttt ttataaaaac tatcgacatt cccattcttt ataatggcaa attgtccgtc
     1681 taattcggca atttctccga taactttttt accgatagtc aatgtataac cttcaaaact
     1741 gtctttacca acattaactt tggcatccgc tacttgaatt tcgatttttt tatctttctt
     1801 gctcatttca tacctctctt cactttttct attttacaag aaaatcctaa aactagcaag
     1861 aaaaaactct atatcaggct tctctgatat agagttttat gtaaacaacc tcaatcgatt
     1921 tagcatagct aatagttagt aaacaagcat aattgactat cctttttagg ttatcagtga
     1981 gtctcaccgc ctactaactg caaatcctta tctttgtagt ctacaatagc ttcaatagct
     2041 acctctatgc ctcttacaat atcacataga ctcattcctg cagtattcgg cttatttact
     2101 acttgttcca tcatatatgg aatatgcatg aaacctgctc tcatattagg gaatttttta
     2161 tccactaaat aaagagcttg gtacatcaaa tgattgcaaa caaaggttcc tgcactattg
     2221 gatacaactg ccggaagtcc ctgttttttg atagcttgta ccatcgcttt gataggtaaa
     2281 ctactaaaat aggccgatgc tccatcaata cgaatcggtg tatcaattgg ttgattgcct
     2341 tcgttatcag gtatgcgagc atcatcttga ttaatagcta ctcgttcagg tgttaagccg
     2401 gtcctgccgc ctgcttgtcc aatacaaagt acagcatctg gttgatatcg taatatttct
     2461 gcctctaaaa cttctgacga cttataaaaa accgttggaa tttctaccca gcgaacttca
     2521 gccccattaa tctcagatgg taataatttt acagcctcca aagctggatt aatcttttca
     2581 cctccaaaag gattaaaacc tgtaaccaat attttcattt tattttcctt tactaaaatg
     2641 cgagaaagta cattaagaat atgtgaataa caatcattac tagagcaaca cctgcttgag
     2701 cctttataac gccattctga tctttcatat ccatcaatgc tgctggtaga gcgttaaaat
     2761 tagcagccat tggggtcaat aaggtcccac aataacctgc tgtcatggca agagcaccag
     2821 ccacaattgg attagctccc agagcaaata caaagggaac tccaacacct gctgtaataa
     2881 cggtgaatgc tgcaaaagca tttcccataa tcattgtgaa tagaaccatt ccaagaacat
     2941 aggccaaaac tcctataaag cgactatctg aaggaacaat accgctaatc agatgagaga
     3001 taacatcacc aacacctgct acagtaaaaa tagcccccaa agcccctaat aattgaggaa
     3061 caataccact tgttgaaact tgctgagtca ttcgattatt ttctgataac agactcttag
     3121 ggtgactatt ggtaatcaca agaacagaaa ttgtagcaaa caaggcggca aggctaatcg
     3181 aaatcttgct aaattctgga atcatttgcg ctaagaccaa cgcaagtatt gccatcagca
     3241 taactggaat aaaaatttta tttttcaacc tgttagattc aatattggct ttcatttcat
     3301 ctaaggatgg caaggttccg atacggactt gcttaaacaa tgttaacagc gataatagga
     3361 ttacaataat accaatactc atatttggca tataggaacc acctataaac gtaatagaca
     3421 atagagtcca aaatgcagat gtcccaagtc gaactgggtt tgttttatct ttataactac
     3481 aataggctgt atggagaaat tgacaaccaa tcacaatata ggtcaactct aatagttgct
     3541 ttgccaactc tgtcattttt gttctcctcc cctagtcttt tttgatatca attttttatc
     3601 aaataaataa ttgtaaatcc ccactacaat aagtgttata acagcaacaa taatagatgt
     3661 agaagcaatc cctgcataat tgctttcata gcctaactga tctaatgttc cccctatcaa
     3721 gaggactccc ccagcaccta caaacgtatt ttgagcaaag aaattcccaa aattttcatt
     3781 cgcagccgca cgcgctttta ttgtctcatc ttcaacctct gttaactttc tacctaattg
     3841 agactctgca gctgcttctc ccataggttg aaccaaaggt ctgacaaact gagggtgtcc
     3901 tcctagacga attgaaaaga aaccagctaa ctctcgaata aagaaataaa ctgtatagaa
     3961 gtttccaact gtcagacctt taatctttcg aatcaaatcg attgatcgtt gcttgagtcc
     4021 aaaggtttct gacagcccca caagaggcaa ggtaaccata aaaatcgtga gcactcgctg
     4081 attgctaaat tcttttccca aaatctccaa aaattcaacg agagaaacac ctgaaactaa
     4141 agctgtaacc aaaccagcta agactactgt tgcaattgta tcaaatttta aaataaaacc
     4201 cacaacaatg attgctattc ctattaatct aatccactcc atatcaaact cctttatatt
     4261 caaaatgaca gtattttaaa aattttatca agatcaatac cattccttat ttaatgtatt
     4321 tttctagttc tttttggtat ttgctattgg attccaattt ttctttttgc cattttttaa
     4381 aaacctcgtt atattctttt gttgtaacaa tatctttttg caatttcatt cctttaaaga
     4441 tatatggttc ccccttaata ccaacttgtg agtatggttt tgagaatggt actacgttac
     4501 ttacaactgg agaaccacca gatgaagctg ttggcatcaa taatgaacta tctgtcgacc
     4561 aagcttgagc tttggcatat ttttcatatc tcttctctag gtcagtggtc tcagaaacag
     4621 catcttctaa caatttctta tatttatcca aaccaggttt agctacaaca tccttatctt
     4681 ttcctttcgt aatgccaagg tgtttcatgg cagaaccaga ttttggatcc ataatattca
     4741 agtaagacgc tggatcttga tagcttggag cccatcctgt actattcaaa tcatagtctt
     4801 tttgagaagg agcaacattg ccgtatttat cattttccat caaaccatca ataacatttc
     4861 caataacgtc tgtcctcgat gttcgagtcg ctatactgta gcccaatgat gctggatcta
     4921 ctgcatagac ataagaaaat gttgtcggtg catctgcttc tttatcagtt tttccacaag
     4981 ccactaaaat agctgacgtg ctcaggacca ctcctgctgt taagagccac tttttctatt
     5041 tcataaaaaa tctcctttgg tttattttaa tctactttta caatccaacc ttctggcgct
     5101 tcaatatcgc caaactgaat acccgtcaat tcattatata atttacgcgt cacaggacct
     5161 acttctgttt cactatagaa tacatggaaa tcatcaccat gttgaatacc tccaattgga
     5221 gaaataaccg ctgctgtacc acaggcacct gcctctacaa aacggtcaag attatcaatt
     5281 ggaacatcac cctcaatagg agttaatccc aagcgatgtt ctgccaaata aagcaaggaa
     5341 tacttggtaa tagatggcaa gatagatgga ctcaatggtg ttacaaattc attatcagct
     5401 gtaattccaa agaagttagc tgatccgact tcttcaatct ttgtatgagt tgatgggtcc
     5461 agatagataa catctgagaa atgacgtgac ttggccattt ttcctggtaa gagacttgca
     5521 gcatagtttc caccaacctt agccgcacct gtaccatttg gtgctgcacg gtcgtactca
     5581 tcctgaatca agaagttggt tgggaccaaa ccacctttaa agtaatttcc aactggcata
     5641 gcaaagatgg tgaaaatgta ctcttctgcc ggttttaccc cgataatatc tccgacacca
     5701 atcaaaagag ggcgaagata taaggttcca cctgttccgt atggtggtac gtattcttca
     5761 ttcgcacgga caactgcttt acaagcttct acaaacatgt ctgtcggaac ttgtggcatc
     5821 aagagacggt cacatgtacg ttgcagacgt ttagcatttt catcaggacg gaacagttga
     5881 acactgccat ccttagtacg ataagctttc aaaccttcaa atgcttgttg tccatagtga
     5941 agacttggag aagactctga aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc
     6001 cattgtccat ttttgaaatg agcaagatag cgataaggta atttcatata ggaaaaaccg
     6061 aggttttccc aatcaatcgt tactgtcata ttccactcct tattctaaaa acctatttct
     6121 tatattctac actatttttc taaaatagca agtatatttt gtaattttca gaaaatttct
     6181 ccaataaaaa ccagctctta gaactgattc ttcatttcac ttatttatct tcagtaacta
     6241 cttcctgaag ataagcgtca aaaacttctt catctgaaat cgtgtcagaa atgaagcttc
     6301 cattgctagt gcgttctgac aagttcaagt cttgcaatcg gctttcatag attgttcctt
     6361 tattggattg gacaagcaga gtttggtcgt tcacatccac ttccgtactg aagaaatcgc
     6421 caacaaatcc ttgctctgca actgctcctg ccaagaagac acgatgcggt ttgtttttca
     6481 actcacgcaa gacttgtaat cctcgtttgg cacggctggt tgctagaatt tcctcaatgg
     6541 aaacacgttt caagcttcca cgctgggtca agaggtagaa ggacgaagta ttacagataa
     6601 agccagattg gaggacatca tcttctttca aattcatagc cttgacacct gctgccttag
     6661 caccgacaac cggaacctct tcgatattga aacgcagggc ataaccattt tgactaacca
     6721 agacaacatc atctagttta atcggagcca ctgctacaat ctgatctgta tcgtctttga
     6781 gcttagcata cttgacagac ttagatctat aggtccgcca tggagtgaat tcttttcgct
     6841 ctacccgttt gatttgacca aggcgagtca ctgcaaagta ggttgtcgca tcgtcaaact
     6901 gatccagtac ttccacataa aggatttctt cattcgtttc aaagtttgtg atggtttggc
     6961 tcagatgctc tccgatgtcc ttccaacgaa tatctgccaa ctcatggatt ggtctgtaga
     7021 tgacatttcc aagacttgtg aacatcaaga ggtgctgggt tgtcttggca gattgaacaa
     7081 aaatcaaacg gtcatcatca cgcttgccaa tttcttccaa ggtggaagcc gcaaaggaac
     7141 gtggactggt acgcttgatg taacctgcct tggtcacgct gacgtaggta tcttcctcag
     7201 cgataagact agctgtatca atctcaattg ctttcgcagt gtcttctaaa gaactcaaac
     7261 gaggagttgc aaatttcttc ttgacctcac gaagttcttt cttcatgaga ttgtacatag
     7321 tcctttcatc accgataata gccgccagca tagcaatctt ctcacgaagc tctgcttctt
     7381 cttcctgcaa gacaaccaca tcggtattgg tcaaacggta cagttgcaaa gttacgatag
     7441 cctcagcctg ttcttccgta aaatcatagc taactttgag gttttccttg gcgtccgcct
     7501 tattctcaga agcacggata agagcaatga cttcatccaa aatcgaaatc acacgaatca
     7561 aaccttcgac gatatggaga cgtttctcag acttttcttt gtcaaagcgt gaacgcgcca
     7621 aaatcacttc tcgacggtga gcgatatagc tagacaggat tggaacaatc ccaatctgac
     7681 gaggtgtgaa attgtcaatc gccaccatat taaagttgta gttgatttgt aggtcggtgt
     7741 acttaaataa gtagttgaga acaagctcag tattagcgtc tttcttaagt tcgatagcga
     7801 tacgaagacc atcacggtca gactcatcac gaacctcagc aatcccagct accttgttat
     7861 taacacgaac atcatcgatt ttcttgacta gattggcctt attgatttca taaggaatct
     7921 cagtaataac gatttgttcc ttaccacctt ttagcttttc aatttcagtc ttggaacgaa
     7981 caaccacgcg ccctttccca gtctcataag ctttcttgat ttcatcacga ccctgaataa
     8041 tagcccctgt agggaagtct ggtccaggca agaattccat gagtttatca atctttgcag
     8101 ttgggtggtc aatcatgtaa actgcagcat ctatgacctc agctaaatta tggggaggaa
     8161 tgtctgtggc ataaccagcc gaaatcccag tcgaaccatt gaccaagagg tttggaaagg
     8221 ctgctggcaa gaccgttggt tctttctccg tatcgtcaaa gttccatgca aaaggaactg
     8281 tctttttctc gatatcctga agaaggtagc ctgcaatttc agacaaacgt gcctcagtat
     8341 aacgcatagc cgcaggagga tctccgtcca tagaaccgtt attaccgtgc atttcaacta
     8401 gaatctcacg atttttccag ttctgtgaca tacgaaccat ggcatcatag atagaagaat
     8461 ccccgtgtgg gtggaaattc cccatgatgt tcccgactga cttggccgac ttacggtagc
     8521 tcttgtcaaa agtattgcta tccttattca tagaataaag aatacggcgt tgaaccggct
     8581 tcaacccatc acgaatatct ggcaaagccc ggtcttgaat aatgtacttg gagtagcgac
     8641 caaagcgctc tcccatgatg tcctccaggg acatgttttg aatgttagac ataagataca
     8701 aagcccataa aataccaagt gaaaatagaa aattcttgaa gtaagcaaac tcacaagaga
     8761 atttatcttt ttcacacagt atctagggcg tgttcaactc ctttcaaaga atgtagagta
     8821 ggtttttatg cagtaaaaga tattttacgg gaattcctcc cgtgttcagt tacgataagt
     8881 aaccaaacta tcctgtttgt atttttcaat atgaaaatct ggttttccaa aattagtctt
     8941 agtttgtgtc ttaaccgctc ccttaagcgc ctctttaaga taagcactca tagcagattc
     9001 ttcattaata atcctgcaat tttttcaaac caagattttc aaactgcttt ttcacatagt
     9061 cattcacatc cgactctaat ttccagttta ctaacatatt attttctttc attaaaacac
     9121 tgtcgcttct tctagcgtaa acttgacatt atcttcaatc catttacggc gtggttctac
     9181 cttatctccc atgagaacat tgacgcggcg ttcggcgcgc gctaaatctt caattgtgac
     9241 acggatgagg gtacgtgttt ctgggttcat ggttgtttcc cagagctggt ccgcattcat
     9301 ctcaccaagt cctttgtatc gttggagggt agcgccttta ccgaactgtt tacggagttc
     9361 ttctagttct ccatccgtcc aagcgtaggc cacttcttct ttcttgcctt tacctttgga
     9421 catcttgtaa agaggtggga gggcaatata gacatgacct gcctcgacta gcggacgcat
     9481 gtaacggtag aaaaatgtca agagcaaggt ctggatatgg gcaccgtcgg tatccgcatc
     9541 ggtcatgata atgatcttat catagttggc atcttcaata gagaagtctg ctccaacacc
     9601 cgcaccaatg gtataaatca tggtattgat ctcttcattt ttgaggatat ccgccatctt
     9661 ggccttggct gtattgacaa ccttaccacg aagaggtaga atagcctgga acttgcggtc
     9721 acgaccttgt ttggcagaac caccggcaga gtccccctca actagataga gttcattctt
     9781 agcaggattc ttagattggg ctggggtcaa tttcccagac aacaagccct tatctttctt
     9841 gtttttcttc ccatttcggc tctcatcacg cgccttacgt gctgcttcac gagcatcacg
     9901 ggccttgata gccttgcgga tgaggttaga agctaattcc ccattttcca taaggaaaaa
     9961 ggtcaactta tcagccacta ttccatccac aactgggcga gctagggggc ttcctagttt
    10021 atccttggtc tgtccttcaa actgcaagtg ttcttcagga actaagatag aaagaacggt
    10081 cgctagtccc tcacgatagt ctgaaccttc aaggttttta tctttttcct tgagaagacc
    10141 tgttttacgt gcatagtcat tcatgacctt ggtaatggca gacttgagtc ctgtctcgtg
    10201 cgttccaccg tccttggtgc gaacgttatt gacaaaggat agaatgttat ctgagaatcc
    10261 gtcattgtac tggagggcta cttccacttg aaaaccattg tcttcccctt caaagtaaag
    10321 aactggcgtc aagatttcct tatcttcgtt gagataagaa acaaaatctt gtactccatt
    10381 ctcatagtgg aactcaatcg cttcatctgt tcgcttgtcc gttaaagaca aggtcacatt
    10441 tttcaagaga aaggctgatt cattaaggcg ctctgaaatg gtattgtact tgaaatctgt
    10501 cgtagaaaag atagtcgcgt caggcataaa agtaactttg gtgcctgttt tagacttggg
    10561 tgctgtaccg attttcttca aagtcgtgac aggttttcca ccattttcaa aacgttgctt
    10621 gtaaactgcg ccatcacggg taatttcaac ttctaaccag ctagaaaggg cgttaacaac
    10681 ggaagaaccc actccgtgaa gtccacctga tgtcttatag ccaccttgac cgaatttccc
    10741 tccggcatga agaatggtaa agataacctc aacagttgga attcccatag cgtgcatacc
    10801 tgtcggcatc ccacgtccat ggtcttgaac cgttagacta ccgtctttat tgatagttac
    10861 atcaatacga tcaccaaacc cagacaaggc ttcatcgact gcattatcaa cgatttccca
    10921 aactaggtga tgaagaccag cgccatcggt cgatccaata tacatccctg gacgttttcg
    10981 gaccgcatcc aacccttcta gcacctgaat agcatcatca ttataattgt taatattgat
    11041 ttcctttttt gacacaagga acctcctatt cgttcatctt tactattcta caggttttcc
    11101 aaggattttg caaaattttt ctttctccga tgtgacaatt ttagcagaga ttctctgctt
    11161 ttctttccca attcatgata taataggagt atgattacaa tagttttatt aatcctagcc
    11221 tatctgctgg gttcgattcc atctggtctc tggattggac aagtattctt tcaaatcaat
    11281 ctacgcgagc atggttctgg taacactgga acgaccaata ccttccgcat tttaggtaag
    11341 aaagctggta tggcaacctt tgtgattgac tttttcaaag gaaccctagc aacgctgctt
    11401 ccgattattt ttcatctaca aggcgtttct cctctcatct ttggactttt ggctgttatc
    11461 ggccatacct tccctatctt tgcaggattt aaaggtggta aggctgtcgc aaccagtgct
    11521 ggagtaattt tcggatttgc gcctatcttc tgtctctacc ttgcgattat cttctttgga
    11581 gctctctatc ttggcagtat gatttcactg tctagtgtca cagcatcgat cgcggctgtt
    11641 atcggggttc tgctctttcc actttttggt tttatcctga gtaactatga ctctctcttc
    11701 atcgctatta tcttagcact tgctagtttg attatcattc gtcataagga caatatagct
    11761 cgtatcaaaa ataaaactga aaatttggtc ccttggggat tgaacctaac ccatcaagat
    11821 cctaaaaaat aaaacgccag ttctgtactg cccccaaaca gttagacaat taatttatcc
    11881 gaaggattta gttctgtatt gcacagggct aagtcctttt agttttacct taattctctt
    11941 attgttgtaa taatcaatat agtctataat ggcttgttcc aattgcttaa gcgactgaaa
    12001 cgacttctca taaccgtaaa acatttccga tttcagaatc ccaaagaagg actccatcat
    12061 actattgtct gggctgtttc ccttgcgtga tatggatgct tgaattccct tactctctag
    12121 gaaccgatga taagaatcgt gttggtattg ccagccttgg tcactatgga gaatcgtatt
    12181 ctc
//
LOCUS       DAYYQN010000056        12015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYYQN010000056 DAYYQN010000000
VERSION     DAYYQN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.057"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            11..1012
                     /locus_tag="ACHBRQ_001637"
     CDS             11..1012
                     /locus_tag="ACHBRQ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9593264.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1683..2051
                     /locus_tag="ACHBRQ_001638"
     CDS             1683..2051
                     /locus_tag="ACHBRQ_001638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593265.1"
                     /translation="MDRTDEVSSKYCFEVVDRTDEVSLKHCFEVADRTDEVSLKHCFE
                     VADRTDEVSLKHCFEVADRTDEVSSKHCFEVADRTDEVSSKHCFEVADRTDEVSNHTY
                     DKATLTRFEEFFEEYKGVPR"
     gene            2423..3784
                     /gene="trpE"
                     /locus_tag="ACHBRQ_001639"
     CDS             2423..3784
                     /gene="trpE"
                     /locus_tag="ACHBRQ_001639"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFM9593266.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3781..4347
                     /locus_tag="ACHBRQ_001640"
     CDS             3781..4347
                     /locus_tag="ACHBRQ_001640"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFM9593267.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4358..5362
                     /gene="trpD"
                     /locus_tag="ACHBRQ_001641"
     CDS             4358..5362
                     /gene="trpD"
                     /locus_tag="ACHBRQ_001641"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFM9593268.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5359..6126
                     /gene="trpC"
                     /locus_tag="ACHBRQ_001642"
     CDS             5359..6126
                     /gene="trpC"
                     /locus_tag="ACHBRQ_001642"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFM9593269.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKKLYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            6113..6712
                     /locus_tag="ACHBRQ_001643"
     CDS             6113..6712
                     /locus_tag="ACHBRQ_001643"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFM9593270.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCVSIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQTFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPHAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6690..7913
                     /gene="trpB"
                     /locus_tag="ACHBRQ_001644"
     CDS             6690..7913
                     /gene="trpB"
                     /locus_tag="ACHBRQ_001644"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFM9593271.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7906..8682
                     /gene="trpA"
                     /locus_tag="ACHBRQ_001645"
     CDS             7906..8682
                     /gene="trpA"
                     /locus_tag="ACHBRQ_001645"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFM9593272.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8654..9313)
                     /locus_tag="ACHBRQ_001646"
     CDS             complement(8654..9313)
                     /locus_tag="ACHBRQ_001646"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9593273.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQIFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFRLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9383..9829
                     /locus_tag="ACHBRQ_001647"
     CDS             9383..9829
                     /locus_tag="ACHBRQ_001647"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9593274.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLDSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYIMKKGR"
     gene            complement(10035..10238)
                     /locus_tag="ACHBRQ_001648"
     CDS             complement(10035..10238)
                     /locus_tag="ACHBRQ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9593275.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLHKEEK"
     gene            complement(10269..10877)
                     /locus_tag="ACHBRQ_001649"
     CDS             complement(10269..10877)
                     /locus_tag="ACHBRQ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9593276.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(10916..11086)
                     /locus_tag="ACHBRQ_001650"
     CDS             complement(10916..11086)
                     /locus_tag="ACHBRQ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9593277.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(11098..11664)
                     /gene="amaP"
                     /locus_tag="ACHBRQ_001651"
     CDS             complement(11098..11664)
                     /gene="amaP"
                     /locus_tag="ACHBRQ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9593278.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(11748..11912)
                     /locus_tag="ACHBRQ_001652"
     CDS             complement(11748..11912)
                     /locus_tag="ACHBRQ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9593279.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT         3654 a         2276 c         2679 g         3406 t
ORIGIN      
        1 gaggactgaa atgaaaaata taaatgggaa aaaggtaact atttatgata ttgcacgttt
       61 atcaggtttt tctccaaaaa cagtttcacg agttattaat ggtggagtaa atgttaaaga
      121 agaaacttat caagcgattc aaaaagttat agaggaattg tcgtatattc caaatgcgta
      181 tgcaaaaaat ttaactaaaa aagaagctat aaatatattg atttctgtaa aaaaaatcga
      241 ctcttttcct ttaatttggt ttcatacgct gttagataaa gtgttacgga cgtgtaaaga
      301 atttggtgtt aatgctatag ttgagtactt tggagaggag gatacaatta gtaattcaat
      361 tatatcaagc acaggtagcc tagtagatgg tgttattgtt ttttatgaaa gtgtagatga
      421 tatcagaatt cagtatttaa agaagaacca tatgcctttt cttgttttcg gtgaatctca
      481 aacgtctgga gtagtctatg tatctaataa taattttcaa gctacttatg atatgatgaa
      541 agctgtaact gaagaaaaat ttaaaaatat gtggttgctt atgggaggag aatctcatgt
      601 taataaggat cgtgaaagag gtgtgcgttc ttttctgaat gataaaaatt actttatgga
      661 tttaaaagtc atttatggct tatctacaat tgattcagtt tattcttatg caatgcagca
      721 cttgactacc caaaattatc cggacattat ttttgtttca ggagatgaaa aggttcaggg
      781 cttgattcgt gcgtgctatg agaaaggaat tcttattcca gatgatattt ctattattgg
      841 atttgataat attcctattt ctcagtatta tactccagct ttgtctacaa ttgctcccaa
      901 ttatgttaaa ttggctaaag aaatgataga aggtgtttta gcaattatta aaggggaaag
      961 tgtcacatct gttgaagttt ctcctaaatt tgttaggaga cagagtttct agtattgttt
     1021 aattcttgaa aatcctagtg gaaaagatgg agtgttctaa aataagttct gtttagcctc
     1081 cattcagttt ctattgagtt ggatccaatt tctaacaagt gggacatgtt catgttcgat
     1141 ctaaggaaga catggtcaca ttcgaatgat attttcaaaa aaatcgtaaa aaagtcttga
     1201 caaagaaaaa acaaagtatt aaaataagtt caacaaatca gaaaagtaac tatttttgat
     1261 cttcagggag cctgtggtga ttgtgaatag gtggttggaa gtagtgaaag tgggctgatt
     1321 ttgaaaatga atttgaaaca atgaaaattc ggtgcgcaca ccttacagtg cagcttgttg
     1381 ttaaacaagg cagagatgta aagggatagt ccctttataa ttgaggtggc accgcgttac
     1441 caacgccctc acacggaagt atattctgtg tgtgggcttt tttctatccg tcgtttggtt
     1501 tatcttttat tagggcctta agtcgctttg atgaacttga gttctatcta cagctccttg
     1561 cctactacta aaagcaaaca aaaaggctgg actaatacga aaaaagctag taatactcaa
     1621 tgaaaatcaa agagtaaact aggaaactag ccgcaggttg ctcaaaacac tgttttgagg
     1681 ttgtggatag aactgacgaa gtcagctcaa aatactgttt tgaggttgtg gatagaactg
     1741 acgaagtcag cttaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagcttaa
     1801 aacactgttt tgaggttgca gatagaactg acgaagtcag cttaaaacac tgttttgagg
     1861 ttgcagatag aactgacgaa gtcagctcaa aacactgttt tgaggttgca gatagaactg
     1921 acgaagtcag ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagtaacc
     1981 atacctacga taaggcgacg ttgacgcggt ttgaagagtt tttcgaagag tataaaggtg
     2041 taccgagatg aacatctctt catcaatgtc tctggtcgtg gaggcaagga cctagctgcg
     2101 attgcagact actagaagct aaaaaataat agatggaaaa attacagttc tttctctcac
     2161 agagagcttg tggttgctga aaacaagcag agaaagctgt aaagttgggt tcatctgaaa
     2221 cgagagaaga aaacatcatt ctcaagggag tgccctttat cgcacagcct gttacaggag
     2281 atatctgaga caggaatgag agataggaga aatcctataa ttgaggtggc accgcgaatt
     2341 tcgtcctcac gcaagttatt ttgcgtgggg atttttcata cagtcaccac ggactagaag
     2401 tagaactgaa agggaaatta caatggaacg aatcattcat ggagatgtct tatcaccaat
     2461 cttggcttat atgcgcctaa aggggcaaca caaggttatc ttagagagta ttccgagaga
     2521 caaggaaaca gctcgttttt ctatcctagc ctataatcct gtttttgaga ttaagtttga
     2581 aaatggagtt ctttatcaaa atggtcaagc gattgatcgg gatcccttgg atttccttta
     2641 tgaagtgact cataagagcc agcaccattc agacctccct tttggtgggg gagccattgg
     2701 ttttgttggt tacgatatga tttcgcttta tgaagaaatt ggtcaaatcc ctgaggatac
     2761 aattgggacg ccagacatgc atttctttgt ctatgagagc tatatggtct ttgaccacaa
     2821 gaaggagaag atacatgtca tagaggatgc actttatagt gagcgcagtc aagaggcctt
     2881 ggaaaaatcc ttgaaccaag tgcttgagga attacgcatt cctgctccaa atgaatttga
     2941 agacttggat ttatcaccac ttgacttcaa accgcatatc gctccacata agtttgaggg
     3001 aatggtagaa acagctcgtg acttgattcg taacggagat atgttccaat gtgtactcag
     3061 ccagcgtttc tcagcagaag ttactggaaa tccatttgac ttctacagaa atctccgcgt
     3121 gactaatcct tctaattacc tttatttcta tgattttggg gattatcaaa tcatcggagc
     3181 cagtccagaa agtttggttt ctgtcaaaaa tggcatcgtg acaaccaatc cgattgcagg
     3241 gacgcgacca agaggggcta cggatgaaga agacaaggcc ttggcgactg acctcctctc
     3301 tgatgagaag gaaacagcag agcatcgaat gttagtagac ctggggcgta acgatattgg
     3361 ccgcatctct gaaacggcca gtgtccaagt tactaagtat atggaggtgg agctcttccg
     3421 ctacgtcatg catttgacca gtgtggtcaa gggacgtttg cttgcagaac tcactgccat
     3481 ggatgccttg aaagcaacgc ttcctgctgg aaccgtttct ggagcaccaa aaattcgagc
     3541 gatgagacgc atctatgaac tggaaacgga aaaacggggc gtatatgcag gagcaatcgg
     3601 ctacttgtct gcgacgggtg atatggattt ggccattgcc atccgaacca tgattctcaa
     3661 aaatcaaaga gcctatgtgc aggctggggc agggattgtc tacgactcta tcgcccaaaa
     3721 cgaataccaa gaaaccatta acaaagcaaa atctatgact agaattggag aactaagacc
     3781 atgattttat tgattgacaa ctatgattct tttacctata acttggccca atacattggg
     3841 aattttgcag aagttcaggt tctgagaaat gatgattcca agctgtatga agaagctgaa
     3901 aaagcagatg gtctggtctt ttctcctggt cctggttggc cagttgatgc tggaaagatg
     3961 gaagacatga tttgtgattt tgcaggcaag aagccgattc tagggatttg tttgggtcac
     4021 caagccattg cagaagtctt tggtggtaag ctaggtttgg ctccaaaagt catgcatggg
     4081 aaacagagca atatcaactt tgaagcgcca tctgttttgt atcaaggtat tgaggatgga
     4141 cgtgcggtca tgcgttatca cagtattttg attgaggaaa tgccagaaga ctttgaagtg
     4201 acagctcgtt cgactgatga ccaagccatc atggggattc aacataaaaa cctaccgatt
     4261 tatggcttcc agtaccatcc agagagcatt ggaacgccag acggcttgtc ttctattcgg
     4321 aattttatcg agaaggttgt aaagtgagga aactaggatg aaagagatta ttgaaaaact
     4381 agcaaaattt gaaaatttat caggtgtgga aatgacggat gtcattgagc gtatcgtaac
     4441 tgggcgtgta actgaagcgc agattgcttc tctcctctta gctcttaaga tgaaggggga
     4501 aacacctgaa gaacgcacag ccattgccca agtcatgaga ggacatgccc agcatattcc
     4561 aactgagatt catgatgcca tggacaactg tggtacaggt ggggacaagt ctttcagttt
     4621 taatatttcc acaactgcag cctttgtctt ggctggtggc ggtattcaca tggccaagca
     4681 tggtaaccgc tcgatttctt ctaaatctgg ctccgcagat gtcctcgaag ccttgggaat
     4741 caatcttgac ctcaaaccag ctgaactagg taaggtcttt gataaaactg gaatcgtctt
     4801 tctcttcgct aaaaatatgc acccagctat gaaatacatc atgccagctc gtttggaatt
     4861 gggaattcca acgattatga acttgactgg tccactgatt catccaatgg ctttagaaac
     4921 acagcttctt ggaattagtc gtccagaact cctagaaagt acagctcagg ttttgaaaaa
     4981 tatgggtcgc aaacgtgcca tcgtggttgc tggaccagaa gggttggatg aagctggctt
     5041 gaacggaaca accaagattg cacttcttga aaatggcgaa atcagcttgt caagctttac
     5101 tccagaggat ttgggaatgg aaggctatgc tatggaagat attcgtggtg ggaatgctca
     5161 ggaaaatgca gaaattttgc ttagcgttct gaaaaacgaa gcaagtccat tcttggaaac
     5221 gacagtcttg aatgctggtc ttggtttcta tgctaatggt aagattgata gcatcaagga
     5281 aggagttgcc ttggcccgtc aagtgattgc tagaggcaag gcccttgaaa aactcagact
     5341 gttacaggag taccaaaaat gagtcaggaa tttttagcac gaatcttaga gcagaaggtg
     5401 cgtgaggtgg agcagatgaa gctggagcaa atccagcctc tgcgccagac ctatcgcttg
     5461 gcagaatttt tgaagaatca tcaggaccgc ttgcaggtaa tcgctgaggt caagaaagct
     5521 agccctagtt tgggagatat caatctcgat gtggatattg tgcaacaggc ccagacttat
     5581 gaagaaaacg gagcagtgat gatttcggtg ttgacagatg aggttttctt taaagggcat
     5641 ttggattatc tacgggaaat ttccagtcag gtagagattc cgacgctcaa caaggacttt
     5701 atcatcgatg aaaagcaaat catccgcgct cgcaatgcag gtgcgacagt tatcttgctt
     5761 attgtggcag ccttgtccga agaacgcctc aagaaactgt atgactacgc gacagagctt
     5821 ggtctggaag tcttagtgga gactcacaat ctagctgaac tagaggtagc ccacagactt
     5881 ggtgctgaga ttatcggggt caacaaccgc aacttgacta cctttgaggt cgacttgcag
     5941 accagtgtag atttagcccc ttactttgag gaaggtcgct attacatttc tgaatctgcc
     6001 attttcacag ggcaggatgc ggaacgacta gccccatact ttaacggaat tttggtagga
     6061 acagctctta tgcaggcaga aaatgtggcc cagagaatca aggagttgca gattgacaaa
     6121 ggttaaaatt tgtggactat cgaccaaaga agcggtggaa acagccgttt cagcaggagc
     6181 cgactatatc ggttttgtct ttgcacctag taaaagacag gtgactttag aagaggcagc
     6241 tgagttggca aagcttattc ctgcagatgt gaaaaaggtt ggagtatttg tttcaccaag
     6301 tcgggtagaa ctgctggaag cgattgacaa agttggcttg gacttggttc aagttcacgg
     6361 tcaggtggca gctgatttat ttgagaattt gccttgtgtc agtattcagg ctgtgcaggt
     6421 agatggaaat gggcatgtcc ccaattctca ggcagactat ctactctttg atgccccagt
     6481 ggcagggagt ggccagacct ttgactgggg tcaactggat acgactggac tagcacagcc
     6541 cttctttatc gcaggtggcc ttaatgaaga taatgtagta aaagcaattc aacattttac
     6601 tccccatgca gtagatgtat cgagcggagt ggagacagat ggacaaaaag atcatgaaaa
     6661 gattagaaga tttatagaga gggtaaagca tggcatatca ggaaccaaat aaagatggat
     6721 tttacggaaa attcggcgga cgttttgtcc cagaaacatt gatgacagca gttttggagt
     6781 tggagaaggc ctaccgtgaa agtcaggcag acccaagttt ccaagaggaa ttaaaccagc
     6841 ttttgcgcca gtacgtagga cgtgaaactc ctctttatta cgcaaaaaac ttgacccagc
     6901 atatcggcgg agccaagatt tatctcaaac gggaagacct taaccataca ggagcccaca
     6961 agattaacaa tgccttggga caagtttggc ttgccaaacg catgggtaaa aagaaaatta
     7021 tcgcagaaac tggtgctggt cagcacggtg tggcaactgc aactgctgcg gccctcttta
     7081 acatggaatg taccatctac atgggtgagg aagatgtcaa acgccaagcc ctcaatgtct
     7141 tccgtatgga gcttttggga gctaaggtcg aggccgtgac agatggttcg cgcgtgctca
     7201 aggatgcggt caatgcagcc cttcgttcat gggtggctaa tatagatgat acccactata
     7261 tccttggttc tgccttgggg cctcatccat ttccagaaat tgttcgtgac ttccaaagtg
     7321 tcattggtcg agaggctaaa caacagtacc gtgacttgac aggacaaaat ctgccagatg
     7381 ccctagtagc ctgtgttggt ggtgggtcga atgccatcgg tctcttccat ccctttgtag
     7441 aagatgagtc agtagccatg tatggagctg aagcggctgg atttggtgtg gatacggagc
     7501 accacgcagc taccttgacc aagggtcgtc caggtgtcct tcacggttcc ctcatggatg
     7561 tgctccaaga tgcccatggt caaattcttg aagccttctc tatctcagca ggtttggact
     7621 atcctggtat cggtccagag cattctcact accacgatat taaacgtgcc agctatgtcc
     7681 ctgtgacaga cgaagaagcc ttggaaggat tccaactctt gtctcgtgtg gaagggatta
     7741 tcccagcttt ggaatctagc catgctatcg cctttgcggt gaaattggcc aaagaacttg
     7801 gaccagaaaa gtctatgatt gtatgcctat caggtcgtgg ggacaaggat gtggttcaag
     7861 tcaaagaccg cttggaagca gatgcagcaa agaagggaga agctcatgcc taagacacta
     7921 acaaaaaaat taaatgctat taaagcagct ggaaaaggaa tttttgttcc ctatatcatg
     7981 gctggagacc acgagaaagg tttggatgga ctaggagaaa caatccactt tctagaagat
     8041 ttgggtgtct ctgcaattga agtgggtatt cccttttcag accctgttgc agatggacct
     8101 gttatcgaag aagctggctt gcgcagttta gcccacggga cctctaccca ggctttagtt
     8161 gaaaccttga aaaccattga aacagagatt ccactggtca tcatgaccta cttcaacccc
     8221 ctctttcagt acggtgtgga gaactttgtc aaagatctgg ctgatacagc tgtgaagggc
     8281 ttgattatcc cagacttgcc tcatgaacat gccaactttg tagaaccatt tttggcagat
     8341 acagatatcg ccttgattcc tttagtaagc ttgaccacag gaattgagcg ccagaaagag
     8401 ttgattgaag gcgcggaggg attcgtctat gccgttgcca tcaatggggt gacagggaaa
     8461 tcaggcaatt accgtgcaga tttggacaag cacttggcgc aacttcatca agtggccgac
     8521 atcccagtct tgacaggttt tggtgtatct agtcaagccg atctggaacg cttcaatgcg
     8581 gtgtcagatg gtgttatcgt cggttcaaaa atagtaaaag ctctccacca aggagagccg
     8641 attcaggatt ttatcagaca agcagtagct taccaaaaat aatcaaacaa gtagcaagta
     8701 agaggaaagg cacgaaagga agtctttcct ttttcttttg caggcgaaag gccaggatac
     8761 ccgtcgcaga agcgaactga atcaagatca gtaactccgt tacgctaaag acgagagcac
     8821 aagaagctaa aaagaggaaa tcccctgcac ccatgcggat atcaataaaa tgagccaaaa
     8881 ttccaagtat gaggaaggag accatgacca gattccagcc agaggaagct attaggatta
     8941 ggtggaaagt catccagacc agtaagggat attcctgatg gtgaaagtcg tatataccca
     9001 aggtcaaacc agcggtgatt aggacgactt gccccaagga aagcaattcc caagagtaaa
     9061 gcagaaagag gagtcctaag cctagttcaa agagggcata ccagacagga tagcgaactt
     9121 tgcagtagcg acagcgaaag cgattgaaga tctgtgagag aatcggaatc aaatctaagg
     9181 gacgcaaggg agtctgacag gaatcgcagt gactggctga actgataatg gattgctctg
     9241 gaaaacggtc aatgaccaaa ccaagaaagg aagctagaat gctcccgacg agaaaaaaat
     9301 aaaaatcaat catacttatc tattcgtaaa aaatgggaga aatagtataa tggagaaaca
     9361 tagataagat ggtgaggaaa agatgacaat tcgttttgaa gaaaaggtga gtatagaaaa
     9421 cgctcagctc gtatgccaat ggtccaactc cttaggcaaa gtctttcaag aacaatggat
     9481 gggaccaagg attccttttc cactgaccct tcaagccttg gaaggagtct tttcaatctt
     9541 tgatgaacaa gagtttgtgg gacttatcca gaaaatcagg ctagaagaca gcaatcttca
     9601 tatcgggaga ttttttatca acccccagaa acaggggcaa ggcttagata gccaggcttt
     9661 aaggaaattt gttagtttgg cctttgaaaa tgaagatata gatagtattt ctctaaatgt
     9721 cttcgaggca aatcaaagag ctcagaatct ttaccaaaaa gaaggatttg aaatcgttca
     9781 aatggttgaa gcacctgtac gaaaatatat catgaaaaag gggagataaa catcaaaaaa
     9841 gccttgattc caaggctttt cctgattgtt atatgacttt cagtctgtct cgctctgtta
     9901 gatgcgaggc aaaaaatcac ccgctattgt actgaccgca acccccaaaa gttagataga
     9961 aaaatctaac ttttgggatg cagttcaata acgttctctt ttttggtctt aaaagaatat
    10021 ataaagtcta aatcttattt ctcttcttta tgaagcatgt ttttaacacc ttctacagca
    10081 ccttctacag cgtctttagc gtcttcggca acttctttta ctttagaaac aactttttca
    10141 gctgctcctt ctttttccat tttttcatca ccgatggctt tcccaacacc ttctttaata
    10201 gaacctttag cttgatttaa tttttcttct actgacatga taatttctcc tgttatattt
    10261 attttatctt agtttacacg agtgttttcg tgagatacta caccatttgc tgcaccttta
    10321 acagcttcta caccttcgct aactttttct tgaacagttg agaaaccaga tccaagacca
    10381 gatttagctt tttcgaattg ttctgaagtg aattctcctg ttgattcagc aacgtcagat
    10441 acgcgatctt gaaggcttac tgagtctgct tcatgctgtt ctttagtttt gatgtcgaca
    10501 acgtttacgt tgatttcaac aatttccaag tcagtcattt tagcaacttc tgaagatacg
    10561 atttctctga tttctgaata taaagctgga acattttttt ggtactcaac aataacgttt
    10621 aagtcaactg caacttgtgt tttaccaact tctacgttaa caccacttgt tacgtcatcg
    10681 ctgttaacga ttttttcttt aagatttgag aagaaaccac catcgattcc caaaagacct
    10741 gaaacgtttt ctagtgaaag accaatgatt ttttggataa ctttatcttc gtaagtaagt
    10801 tcccctttga tttcgtgagc tacaacagta gcatctttct tttctacgtt agtgtttgta
    10861 tttttttcgt ttgacatatg aaactccttt aattaaatta gtaattttta tttttttatt
    10921 tatctatata gtttttttcg atatataatc cagctgcaac tcccagtgct cctaaaatca
    10981 atacaaatat tgttttgaag aagccaaagg agacaatcaa acaagccaga aatacgccta
    11041 tgagaccagc gataattgga tatcgatatt gtttaagcca ttccattttt tacttcctta
    11101 cttcacacga ctaacagtct ttttgttttg aggttttggt tggtaatttt ttactgcaac
    11161 ttcaagtttt acttgacgct caataccaaa aaactgcttc aatccattag ttatttcatt
    11221 ttgaattatt tggcatctgt cagcgatgtt gtctgaagga agaattttac cttctacatg
    11281 aacgaaacat ttattttttc gtaaatttac atgaacagtt gggttcttga tcaatctatg
    11341 atcactcacc aaacttctaa caaaaccttc gattgccgaa ttctttaatt ttaatgtatc
    11401 gtttttagtt tcaagttgga tttccaagta acgtttagga taaaacatca caactaaaat
    11461 ggataataaa actaaagttg atagaaccac tgtcccccaa aagacatatc tagcaagata
    11521 gaattcaact acggagttct gtctccagct aagtagatga atacctagat cactaacttg
    11581 atgataatct atcaaaacag gaaggaaaat tgtcaagatt aaaatacaga aaataatgaa
    11641 acatattttc tttgcttttg acatatacaa tcctttcgat ttaacattta aactattttt
    11701 cccgtcattg atttaaaaat agtttgctaa ctttactggc gaaaagttta ctttctacct
    11761 gtaaagaatg acactacagt aatcacaatc gctgcaccta caatagatgg gagcaaagcc
    11821 attccagcga ttgatggacc ccaactacct aaaagagatt gtcctgcata tgccccgact
    11881 aaaccagcga atacatttgc gatgattccc atagaactac ttttttagtg attttacctg
    11941 ccaagagacc aataagacct ccagcaataa ttgatcacaa cactgcctac cctccttttt
    12001 tattttaata tcaaa
//
LOCUS       DAYYQN010000057        11678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYYQN010000057 DAYYQN010000000
VERSION     DAYYQN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.058"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            110..763
                     /locus_tag="ACHBRQ_001653"
     CDS             110..763
                     /locus_tag="ACHBRQ_001653"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9593280.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            773..1237
                     /locus_tag="ACHBRQ_001654"
     CDS             773..1237
                     /locus_tag="ACHBRQ_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9593281.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1250..2518
                     /locus_tag="ACHBRQ_001655"
     CDS             1250..2518
                     /locus_tag="ACHBRQ_001655"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9593282.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     NATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2532..2792
                     /locus_tag="ACHBRQ_001656"
     CDS             2532..2792
                     /locus_tag="ACHBRQ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593283.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2803..4164
                     /gene="pdrM"
                     /locus_tag="ACHBRQ_001657"
     CDS             2803..4164
                     /gene="pdrM"
                     /locus_tag="ACHBRQ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HFM9593284.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4318..5286
                     /locus_tag="ACHBRQ_001658"
     CDS             4318..5286
                     /locus_tag="ACHBRQ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFM9593285.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5302..6294
                     /locus_tag="ACHBRQ_001659"
     CDS             5302..6294
                     /locus_tag="ACHBRQ_001659"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9593286.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6512..7555
                     /locus_tag="ACHBRQ_001660"
     CDS             6512..7555
                     /locus_tag="ACHBRQ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HFM9593287.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7601..9304
                     /gene="lpdA"
                     /locus_tag="ACHBRQ_001661"
     CDS             7601..9304
                     /gene="lpdA"
                     /locus_tag="ACHBRQ_001661"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9593288.1"
                     /translation="MALEIIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAALPEASPVPVASTS
                     NDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHSAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            9368..10357
                     /locus_tag="ACHBRQ_001662"
     CDS             9368..10357
                     /locus_tag="ACHBRQ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9593289.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="ACHBRQ_001663"
     CDS             complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="ACHBRQ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9593290.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAKNIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3455 a         2193 c         2632 g         3398 t
ORIGIN      
        1 tcaaatcgta tatatagtcg atttattaaa tctttagata aactgtaaaa agtggtttcc
       61 atagccactt tttgctataa tagaggcagt aaaaatgaag gagtcagcta tggaacactc
      121 gtcttggcat gctttgatta aggcgcaatt acctgagggt tatttcggga aaatcaatca
      181 gtttatggag caggtctatt ctcaggggat tatttatcca cccaaggaaa aggtttttca
      241 ggctctcttg acaacactgc ttgaagaagt taaggtggta attctagggc aagaccccta
      301 tcacggacca ggtcaagcgc agggcttgag tttttctgta cctgactcta tcccagctcc
      361 accatccttg caaaatatct tgaaagaatt gtcagatgat atcggagtta agaaatctca
      421 tgatttgaca gcttgggctg agcaaggagt cttgcttctt aatgcttgtt tgactgttcc
      481 tgctggacag gccaatggtc atgctggtca aatctgggag ccttttactg atgctgtgat
      541 tcaggtggtc aatcatctag atagaccagt cgtttttgta ctctggggag cttatgcacg
      601 taagaagaag gccttagtta ccaatcctca tcacttgatt attgaatcag cccatccaag
      661 tcctttgtcg gtttatagag gattttgggg ttccaagccc ttttccaagg ccaatgcatt
      721 cttaaaagag acaggacaag agccaatcga ttggcttaga taaggagaaa atatgcctca
      781 gttagcgacg atttgctaca ttgataatgg gaaagaactg ctcatgctcc atcgtaataa
      841 gaaacccaat gatgtccatg aagggaaatg gattggtgtg ggtggtaagc tagagagagg
      901 agagacgccc caggaatgcg cggcgcgtga aatccttgaa gaaacagggc tcaaagccaa
      961 gccagttcta aaaggtgtca tcacttttcc tgaatttaca ccagatttag actggtacac
     1021 ctatgttttt aaggtgacgg agtttgaggg cgacttgatt gactgcaatg aggggacgct
     1081 agaatgggtt ccctatgacg aggttttgag caagccgact tgggaaggtg accacacctt
     1141 tgttgagtgg cttttagagg ataaaccctt cttttcagcc aagtttgttt atgatgggga
     1201 taaattgttg gatacccaag ttgatttcta tgaataaagg agaaagcaga tgctactaat
     1261 caaaaatggt cgtgtaatgg atcctaagtc tggtttggat caagtttgtg atgtcttagt
     1321 tcaagatggg aaaattatca aaattgcgcc tgagatcacg gaagaaggag cagaaacgat
     1381 taatgctact ggtcttgtgg ttgctcctgg tttggtcgat attcatgtgc atttccgtga
     1441 acctggtcaa acacataaag aagacattca tactggtgcc ctagcagccg ctgcaggtgg
     1501 ttttactact gtcgtcatga tggctaatac cagtccaacc atttcagacg tggagacttt
     1561 gcaagcagtt ctccagtcag ctgccaaaga gaagattaat gtcaagacag ttgcgaccat
     1621 tactaaaaac tttaatggta aaaacttgac tgactttaag gcgctcttag aagctggtgc
     1681 ggttggtttc tctgatgacg gtattccgct tgagagcagt aagattgtca aggaagccat
     1741 ggaggaagcc aaaaaactca atacctttat cagccttcat gaggaagatc caggtttgaa
     1801 cggtgttctt ggctttaatg aaaatattgc tagagaacat tttcatatct gcggtgctac
     1861 tggggtggct gagtacgcta tgatggcgcg tgatgtcatg attgcctatg caactaaagc
     1921 ccatgttcac atccagcatt tgtctaagga agaaagtgtt aaagtagtgg agtttgctca
     1981 ggggttaggt gcagaagtca cagcagaagt agcgccacag catttctcta agacagaagc
     2041 acttctttta acacaaggta gcaatgctaa gatgaatcca ccgcttcgtt tggaatcaga
     2101 ccgtcgtgcc gttatcgaag gtctcaaatc aggtgtcatc acagttattg cgactgacca
     2161 cgcgcctcat catgtagatg aaaaaaatgt tgaggatatt accaaagcgc catctggtat
     2221 gactggctta gaaacatccc tgtctctcag cttgacctat ttagtagaag ctggtgagtt
     2281 gagcttgatg gaattacttg aaaaaatgac atacaaccca gccaagcttt acaactttga
     2341 agcaggttac ttggctgaga atggtccagc agatatcact atttttgatg ccaaggctga
     2401 ccgccttgtg gactcccatt ttgcttccaa agcagctaat tcaccattca tcggtgaaac
     2461 cttaaaaggg caggttaaat ataccatctg taagggacaa atcgtctatc aagcttgata
     2521 ggagttagaa tatgaattat tttcgaaaac gtagggagag acaagcgaaa tctaatagtg
     2581 gaatttactg tcccgcagct aatcgttcca aaattcaata tgaaactaaa gaaaaagctg
     2641 accatgcggt tcaatatgat aacggaggtt tagttagaag ctattattgt cgtacatgtg
     2701 cgtgttggca tactacttca aaatctaata aacctccgct aagtttgaaa aactatggtc
     2761 tgaaactctt tggtttagat aaatcagaat agaaagagaa gtatgtataa gacaaagtgt
     2821 ttacgagaga agttagtatt atttttaaaa attttcttcc caatactgat ctaccaattt
     2881 gccaattatt ctgcctcttt tgttgatact gcaatgacag gtcaatacaa cactatggac
     2941 ttggctggtg tatctatggc aaccagtatc tggaatcctt tctttacatt tctaacaggg
     3001 attgtgtcag ccttggtgcc tatcattggt caccatcttg gtcgaggaaa aaaggaagaa
     3061 gttgcgtctg atttttacca atttatttat ttggccttgg gcctatctgt ggtcttgctg
     3121 gggatggtac ttttcttggc accaacaatc ttgaatcata ttgggttaga agcagcagta
     3181 gcggcagtag cggttcgcta tctttggttt ttatctatcg ggattatccc cttgttgctc
     3241 tttagcgtca ttcgttcctt gctggattcg ctgggcttga ccaaactgtc catgtacctc
     3301 atgcttttgt tactccctct caatagcgga tttaactatc tcttgattta cggtgccttt
     3361 ggtgttccag aactgggagg ggctggtgct ggtttaggaa catccttggc ctactgggtc
     3421 ttgcttggga tttctgttct ggttttattt aaacaggaga agctcaaagc cttacacctt
     3481 gagaaacgaa ttccacttaa tatggataaa attaaggaag gagttcgttt aggtctgcct
     3541 attgggggaa ctgtcttcgc ggaagtggct gtcttttcag tggttggctt gattatggct
     3601 aagttttcgt ccttgattat agctagtcac cagtcagcta tgaacttttc aagtcttatg
     3661 tacgcctttc ctatgagtat ctcatcggct atggctattg tcgtttccta tgaagtggga
     3721 gccaagcgat ttgatgatgc gaaaacctat attggtctag gaagatggac tgccctcatt
     3781 tttgcggcct tcaccttaac cttcctttac atttttaggg gaaatgtggc cagtctttat
     3841 ggtaacgacc caaaatttat cgatttgaca gcgcgttttt taacttatag tcttttcttc
     3901 cagttagcag atacctttgc ggcgccgctt cagggaattt tgcgggggta taaggataca
     3961 gttattcctt tttaccttgg tttgcttggt tattggggcg tagcaatccc tgtggctacg
     4021 ctatttgatt ccctaacaga ttttggagct tattcttact ggatcggctt gattattagt
     4081 ttgattgtga gcggggcgct ctaccgttgg cgtttaactg tgattatgaa gagatttgaa
     4141 tctttagcaa aatccaaacg ctaaaaagtt tgtgaaaaaa tattcttgac aagaaaatcc
     4201 cttgttttca cggggttttc ttttttattt tgtgaaattt cttttagaaa aactttgaca
     4261 gtatttgcca aaagcggtaa aatagtaagg taagaagaaa gttagaaagg caagaagatg
     4321 tcaactttag ataaaaatct tttgctagaa atgttccgta agatggaaga aatccgtcgc
     4381 atggacttaa aaattgcaca attagtaaag aaagggaaag tgccaggaat gacgcacttt
     4441 tctgttggtg aagaggcagc taacgtgggg gctatgttag ctctcaatcc agatgatctg
     4501 attacctcaa accaccgtgg acacgggcaa gctattgcta aagggattga cctcaacgga
     4561 atgatggctg aaatccttgg taaatacact ggaacctgta aagggaaagg tggatctatg
     4621 catatcgctg accttgatgc tgggaacctt ggtgccaatg gtatcgtagg tggtggtatg
     4681 gggatcgctg tcggtgcagc cctcagtcag caaatgcaaa ataccggtaa aatcgttgtt
     4741 tgcttctttg gagatggtgc gaccaatgaa ggtgttttcc acgaagcagt gaacatggct
     4801 tctatctgga acctgccagt cattttctat tgcattaaca acggttacgg tatctctgcg
     4861 gatatcaaga aaatgaccaa tatagaacat atccatcaac gtagcgccgc ttatggaatt
     4921 cctggaatgt tcatcgaaga cggtaacaat gtcatcgatg tctatgaagg atttcagaaa
     4981 gctgtagacc atgttcgcag tggcaatggt tcagtcttga ttgaaagtgt aacttatcgc
     5041 tggctcggtc actcatcatc tgaccctggt aaatatcgta cgcgtgaaga agtggaattg
     5101 tggaaacaaa aagatccaat cgaaaacctc cgcaattacc ttattgaaaa taacattgca
     5161 agtgccgaag aattggaaga aatccaagcg caagtaaagg aagcagtaga agcttctgtt
     5221 aaatttgcag aggaaagccc attcccaccg cttgaatcag cctttgaaga tatttacgca
     5281 gactaaggag aaagagataa aatggaaaca aaaacaatgt ccttccgtga caccattatc
     5341 cttgctatgt ctgaggaaat gcgtcgcgat gaaaatgtgt tcttgatggg agaagatgtc
     5401 ggtgtcttcg gaggagactt cggtacttct gttggaatgc ttgaagaatt tggtccagaa
     5461 cgtgtccgtg actgtccgat ttctgaagct gccatctctg gagcagcagc aggagcagcc
     5521 atgacaggac ttcgtccaat cgtcgatatg accttcatgg acttctctgt tattgccatg
     5581 gacaatatcg tcaaccaagc tgctaaaaca cgttacatgt ttggtggtaa aggtcaggtt
     5641 ccaatgactg ttcgttgtgc agctggtaac ggagttggct ctgccgccca gcactcacaa
     5701 tctctagagt cttggtttac tcacattcca ggacttaagg ttgtggcacc tggaacacct
     5761 gcggacatga aaggcctgct taagtcttct atccgtgata acaacccagt tattatactt
     5821 gagtacaagt cagaatttaa ccaaaaaggg gaagtgccag ttgatccaga ctacacaatt
     5881 ccacttgggg ttggtgaaat caaacgccaa ggaacggatg taacagttgt tacttatgga
     5941 aaaatgcttc gccgtgtggt tcaagctgct gaagaattag cagaagaaga aatttcagtt
     6001 gaaattgttg acccacgtac ccttgttccg cttgataagg atatcatcat aaactcagtg
     6061 aagaagactg gtaaggttat tctggtcaac gatgcccaca aaacaagtgg ctatatcggt
     6121 gaaatttcag ctattatttc agaatcagaa gcatttgact atctagatgc accaatacgc
     6181 cgttgttcag gagaagatgt gccgatgcct tatgcacaaa acctagaaaa tgcaatgatt
     6241 ccaacagttg aaagtatcaa agatgcaatc cgaaaaactt ataacaaaga ctagagttac
     6301 ataatataag ttatgtaacc aaaataaagc tgacgagaaa ctttgtatct tttaggtgca
     6361 gagctcttcg tcattaatat cttatattcg agcacgggct aaaagctcgg aaaaaagata
     6421 aatctccttg gcttcatcga agccatcgtc gatttcctat ttttcagtcg cttttgacgc
     6481 cctttgtatc ataaatagaa ttggagaggt catggctgat gacaagctaa gagcgactcc
     6541 tgcagctaga aagttagcgg atgatttagg gataaacctc tacgacgttt ctggctcagg
     6601 tgcaaacggt cgtgtccaca aagaagacgt ggaaacttat aaagacacaa acgtggttcg
     6661 catttcgcca cttgcaaaac gaattgccct cgaacataac attgcttggc aggaaatcca
     6721 aggaaccggt catcgtggta aaatcatgaa gaaggatgtt ttggccctgc ttcctgaaaa
     6781 tatcgaaaac gatagcatca aatctcctgc tcagattgaa aaagtggaag aagtccctga
     6841 taacgtaaca ccatatggtg aaatcgagcg tattccaatg acaccaatgc gtaaggttat
     6901 tgcccaacgc atggttgaat cttacttgac tgcgccaacc ttcaccctca actatgaagt
     6961 tgatatgact gaaatgttgg ctcttcgtaa gaaggttctt gagccaatca tggaagcaac
     7021 tgggaagaag actactgtaa cagaccttct ttcgcttgca gttgttaaga ctcttatgaa
     7081 acacccatac atcaacgctt cattgacaga agatggcaag actattatca ctcacaacta
     7141 tgtcaatctt gccatggcag ttgggatgga taatggattg atgacacctg ttgtttacaa
     7201 tgctgagaag atgagtcttt cagaactggt ggtagccttt aaggatgtta ttggccgtac
     7261 cttggatggt aaattggctc caagtgagtt acaaaattca acattcacaa tcagtaattt
     7321 gggaatgttt ggtgttcagt cctttggtcc gattattaac caacccaact cagctatcct
     7381 tggtgtcagt tcgacaatcg agaagccagt tgtcgtcaat ggtgaaattg tgattcgccc
     7441 aatcatgagt ttaggattaa ccattgacca ccgtgtcgta gatggtatgg ctggtgctaa
     7501 gtttatgaag gacttgaaag agttgattga aacgccaatc tcaatgttaa tttaagagca
     7561 agagtattca tttttaagat atagataaag gaaggaagac atggccttag aaataattat
     7621 gccaaaagcc ggcgtggata tgacagaagg acaaatcgtc caatggaata aaaaagtcgg
     7681 agaatttgta aaagaaggag aaatcctttt ggaaatcatg actgataaag tcagcatgga
     7741 attggaagcc gaagaagatg ggtacttgat tgccattctc aaaggagatg gtgaaactgt
     7801 ccctgtaacg gaagttatcg gttaccttgg tgaagaaagg gaaaacatcc caacagctgg
     7861 agcagcattg ccagaagcta gccctgtacc tgtagcaagt acctcaaacg atgatggtaa
     7921 gagcgatgat gcttttgata tcgttgtgat tggtggaggt cctgctggtt atgttgcagc
     7981 cattaaagct gcccaactcg gcggtaaggt tgcccttgtt gagaaatctg aacttggtgg
     8041 aacctgctta aaccgtggat gtattccaac caagacctac cttcatagcg ctgaaattat
     8101 tgaaaatatc ggtcatgctg caaatcgtgg tatcgtgatt gaaaatccta acttcactgt
     8161 tgatatggaa aaacttttag aaactaaatc taaagttgtt aatactcttg ttggtggtgt
     8221 tgcaggactt cttcgtagct acggagttgc tgttcataaa ggaattggta caatcactaa
     8281 agacaagaac gtcttggtaa atggttctga attgcttgaa accaagaaaa tcattcttgc
     8341 tggtggttca aaagtcagca agatcaacgt ccctggtatg gaatctccac ttgtcatgac
     8401 tagtgatgac attcttgaaa tgaacgaagt gccagaaagc cttgttatca tcggtggtgg
     8461 agttgtcggt atcgaactcg gtcaggcctt catgacattt ggttcaaaag tgactgttat
     8521 cgaaatgatg gaccgtatag ttccagctat ggatgtggaa gtttctaaga accttcgctt
     8581 gatccttgag cgtaaaggaa tgaccatctt gactggtact aaactgcaag aaatcattga
     8641 ggaaaatggt caacttcgta tcaaggttga aggaaaagac gatatcatcg caagcaaagc
     8701 tcttctttca attggtcgta tgccagacct tgaaggtatt ggagaggttg agtttgaatt
     8761 ggatcgtggt tgtatcaagg tcaacgaata catggaaact tcagttccag gcatttatgc
     8821 accaggtgac atcaacggta ctaagatgtt ggctcacgca gctttccgca tgggtgaagt
     8881 ttccgctgaa aatgccctta aaggaaatca tgcagttgcc aaattgaatt tgactcctgc
     8941 agccatctac actcttcctg aagtagcagc agtaggtttg acagaagaac aagcccgtga
     9001 gaaatacgat gttgccatcg gtaagtttaa ctttgctgct aacggtcgtg ctattgcatc
     9061 tgacgcagct caaggtttcg taaaagttat cgctgataag aaatacggag aaatccttgg
     9121 tgtacacatt attggtcctg cagccgcaga attaataaac gaagcatcaa gcatcatcga
     9181 aatggaaatc actgttgagg aaatgctgaa gaccatccac gggcacccaa cttactctga
     9241 agtgatgtac gaagcgtttg cagatgttct aggaatggcc atccattcac ctaagaaaaa
     9301 ataaaacgaa gatagattag actggaaggt tatttccagt ttctatcata taaagtaaag
     9361 ggatatcatg aaatacatta tcaatcattc aaatgacact gcttttaata ttgccttgga
     9421 agaatacgcc tttaaacacc ttttggatga ggatcaaatc ttcctacttt ggattaacaa
     9481 gccatctatc attgttggtc gtcaccagaa cactattgaa gaaatcaacc gtgattatgt
     9541 tcgcgaaaat ggtattgagg tagttcgccg tattagtggt ggtggagctg tttaccacga
     9601 tttaaataac ctcaactaca cgatcatctc caaagaagat gaaaataagg catttgactt
     9661 caagagcttc tcaactccgg ttatcaatac cttggctcaa ctcggggtta aagctgagtt
     9721 cacaggtcgt aatgaccttg agattgatgg taagaagttc tgtggcaatg cccaagccta
     9781 tatcaacggc cgtatcatgc accacggttg cttgctcttt gacgttgatt tgtcagtcct
     9841 cgcaaatgcc cttaaggttt caaaagataa atttgaatca aaaggtgtga aatccgtccg
     9901 tgcccgtgta accaatatta tcaatgaatt accaaaaaaa atcacagtcg aaaaattccg
     9961 tgatttactc ttggaataca tgaaaaaaga gtacccagag atgactgaat acgtcttttc
    10021 agaagaagaa ttggccgaaa tcaatcgcat caaggatact aagtttggaa cttgggactg
    10081 gaactatggt aaatcacctg aatttaacgt ccgtcgtgga accaaattca ctagtggtaa
    10141 ggttgaagtc tttgctaacg ttactgaatc aaaaatccaa gacatcaaga tttatggtga
    10201 cttctttggt attgaagacg ttgctgcagt agaagatgtc cttcgtgggg tgaaatacga
    10261 acgcgaagat gttcttaagg cgctaaaaac cattgatatc acacgctact tcgctggtat
    10321 tagccgtgaa gaaatcgctg aagcggtagt tggataaatc aaaaagaagt tggccaacta
    10381 accaacttct ttttttgata tttatttaca taatttaaaa tacgtaaaat cataaactat
    10441 ccagagcatt cttttgttca tcactaacaa tatgggtata gaggtcagtg acttgtgtgc
    10501 tagcatgtcc tagttggtga ctgactaaaa cttgtgattt agtcgcatca tagagcctag
    10561 ttgctagtgt atggcgcagt ttatggggtg ttacacgcac tttaaaatcc tctgagtatt
    10621 tagcaaccat tttctcaacg ctagaagcat cgatacgatt aggaacacct ctgtagagag
    10681 ttaaaaaaag ggctgtatct gttttttccg ttttatagcg ttgattccga atggccagat
    10741 aattctctaa ataaggttta gcaaaagcag cgacattgac tgagtcacgt ttgccacctt
    10801 ttcgagtaac atcaataacc atcattttta gattgagatc tcttagatct agattaacag
    10861 cttcagataa gcgaacacca gatgccaaga gaagggcaat aatggctaaa tctcgttctt
    10921 tatttttgtt gaatgatgag agagctcgat ttgaaagctg ttgtgggtgc tcttgatcga
    10981 tataagttag aaaaccttct gtttcatcac ctagaaagag tttttgcttg atatttttag
    11041 ctctggcagc aagggtttct ttctttttct tggttgaaac ttttttcatt acattacgat
    11101 agaaataagg ttccccctga tcgttttcaa cctcttcggt tagatacttg taaagactag
    11161 aaagtgctga taaggttcga ttgatagttg tctgtgaaac accttgtttt gttgtattag
    11221 cattcagcaa gggacgttca cgtagataaa ggataaagga ttccatgtct ttcttagaca
    11281 tattttccaa aactgataaa ggaatatcgg atattttatc agcgtttgaa ataccagact
    11341 ccaaaaccca gctgaaaaat cggtcatatt ccttaaggta ttcgtacagg gttgtaaaac
    11401 tgtagggcac agccagctta gattggtagt attccagaac ataccagggc atgagttgtt
    11461 ttagtttgtc gattcgttcc agtaaaatct cacgtttcat gtattttctc cataagtaag
    11521 tatactagta gtatagcata gacttgtata tttttcaaga aaattatagt ttttcagaaa
    11581 gatgttatag gagtttttca aagtgataaa aagacttaga cctacaaaca aaacagaata
    11641 aggaaaataa taaaaatctc acccactaaa cttctgtt
//
LOCUS       DAYYQN010000058        11527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYYQN010000058 DAYYQN010000000
VERSION     DAYYQN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.059"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..432
                     /locus_tag="ACHBRQ_001664"
                     /pseudo
     CDS             1..432
                     /locus_tag="ACHBRQ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            605..802
                     /locus_tag="ACHBRQ_001665"
                     /pseudo
     CDS             605..802
                     /locus_tag="ACHBRQ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            830..1246
                     /locus_tag="ACHBRQ_001666"
     CDS             830..1246
                     /locus_tag="ACHBRQ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HFM9593291.1"
                     /translation="MKAALNSLKDVQANASDSKSAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLVRRKKESEIQQLSTELTKILGQLDAEKVDKKVLAKA
                     QNLLQETLDFVKEENGSAETEAKLVEELKAILAKLK"
     gene            1475..2182
                     /gene="ccdA2"
                     /locus_tag="ACHBRQ_001667"
     CDS             1475..2182
                     /gene="ccdA2"
                     /locus_tag="ACHBRQ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HFM9593292.1"
                     /translation="METIVFSISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHFKKLEVQKSFTFKKSEANRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMAIPFLVLALASGVVMPYFSKIKRHMMLLKKIGGFLIILM
                     GILLILGQVNVLAGIFE"
     gene            2193..2750
                     /gene="sdbB"
                     /locus_tag="ACHBRQ_001668"
     CDS             2193..2750
                     /gene="sdbB"
                     /locus_tag="ACHBRQ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HFM9593293.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAPQQPKQQTPVQQIA
                     VGKEAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILSVIAPGIQGEKTVEQFPQWFQDQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGVISNADAEAAFKEMN"
     gene            3128..4519
                     /locus_tag="ACHBRQ_001669"
     CDS             3128..4519
                     /locus_tag="ACHBRQ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9593294.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4662..5579
                     /gene="adcAII"
                     /locus_tag="ACHBRQ_001670"
     CDS             4662..5579
                     /gene="adcAII"
                     /locus_tag="ACHBRQ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HFM9593295.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5587..8148
                     /gene="phtD"
                     /locus_tag="ACHBRQ_001671"
     CDS             5587..8148
                     /gene="phtD"
                     /locus_tag="ACHBRQ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HFM9593296.1"
                     /translation="MKINKKYLAGSVAVLSLSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTVRGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKIDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVPSDKVKLVDDILAFLAPIRHP
                     ERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWI
                     KKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRM
                     PYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHP
                     NERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHP
                     ESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEA
                     LLEKVTDPSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIP"
     gene            8358..11477
                     /gene="phtE"
                     /locus_tag="ACHBRQ_001672"
     CDS             8358..11477
                     /gene="phtE"
                     /locus_tag="ACHBRQ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HFM9593297.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         3807 a         2081 c         2372 g         3267 t
ORIGIN      
        1 ataatccaga cgcttatttg actgaaatag cttctgaatt tggctgtcat ccaactacca
       61 tccactatgc gctcaaagct atgggctaca ctcgaaaaaa agaaccacac ctactatgaa
      121 caaaacccag aaaaagtagc cttatttctt aagaatttta atagtttaaa gcacctagca
      181 cctgtttaga ttgatgaaac aggatttgat acttattttt atcgagaata tggtcgctca
      241 ttaaaaggtc agttaagaag aggcaaagta tctggaagaa gatatcagag gatttctttg
      301 gttgcaggtc taacaaatgg tgaattaatc gctccaatga cttacgaaga gacgatgacg
      361 agcgactttt ttgaagtatg gtttcagaaa tttctcttac caacattaac cacaccatcg
      421 gttattatag taaaatggaa taagaacagg acaaatcgat caggacaatc aaatcaattt
      481 ctagcaatgt tttagaagtc catatgtact attctagttt caatatacca taactgctcc
      541 tagtcaacct gcaagtcctg ccccagctcc aagttcagct ccgactcaag ctcctagagc
      601 ggcagcaagc gcttcggcta gctcaacgag cacgcaagct ccagctcaag agcaagttga
      661 taagtctgaa cttcgtgcct tgactcaaga gttagaccaa cgcttgaaag ctttggcaac
      721 agtatctgat ccgaaaatcg atgcagccaa agctatcctc ctagatgctc aaaaagcttt
      781 ggaagatagt tccttgacag agcaaggtct gcgtactgca gtagagtctg tgaaggctgc
      841 cttgaactct ctcaaagatg tgcaagcaaa tgctagcgac agcaagtctg cacaggacaa
      901 gaaagatgct aagcaaggaa cggaagatag taaggattca gataagatga ctgaaacaaa
      961 ctcagttcca gcaggagtga ttgtagtcag tctacttgct ctcctaggcg tgattgcctt
     1021 ctggttggtt cgccgtaaga aagagtcaga aatccagcaa ttaagcacgg aattgaccaa
     1081 gattctcgga cagctagatg cagaaaaagt agataagaaa gtccttgcca aagcccaaaa
     1141 ccttctccaa gaaacccttg atttcgtgaa agaagaaaat ggctcagcag agacagaagc
     1201 taaactagta gaggagctta aagcaatcct tgccaaactc aagtaaaaca tgagttatga
     1261 aatgtcaaat ctaaaagacg tttccgttca caaaagcagc gacgagtaac ccttgtcgtt
     1321 gctttttggc tattttgggg gataagatcc gccttgacta gccagaaaag attagtccaa
     1381 tagtgcccga tacgattttg cgtagcagaa agcttacaaa taaaattgat tatagtaaaa
     1441 taggattaga aatcttaaga aagttggttc tgtattggaa acgatagtat tttcaatctc
     1501 tgtttttcta gcgggtgttt tatccttttt ttctccttgt atttttcctc ttctgccagt
     1561 atatgctggg attttattgg atgatcagga aagcgcaaaa agtttttcct tgtttgggag
     1621 aaaggttctc tggtcaggct tgattcgaac actttgcttt atcgcaggta tttcactcat
     1681 tttctttatt ctaggctttg gtgctggtta ctttggtcat attctctatg caaattggtt
     1741 tcgatatgtc atgggagcta ttattatcat tttgggtctt caccagatgg aaatttttca
     1801 ttttaagaaa ttagaggttc aaaaaagctt tacttttaag aaatcagaag ccaatcgtta
     1861 ttggtcagca tttttacttg gtattacctt tagctttggt tggacgcctt gtattggtcc
     1921 agttttaagt tctgttttag cacttgcggc ttctggtggt aatggagctt ggcaaggagc
     1981 catctatact ctcatttaca ctctggggat ggccattcct ttcttggttt tggctctagc
     2041 ttcaggagta gtgatgcctt attttagtaa aatcaagcgt catatgatgc tactgaagaa
     2101 aattggtggt ttcctcatta ttttaatggg aattttgtta atactgggac aagtaaatgt
     2161 tctagctgga atttttgaat aaaggagaac aaatgaaaaa agtaatgttt gctggcttaa
     2221 gtctcttgtc attagtcgta ttgatggcct gtggtgagga agaaactaaa aagactcaag
     2281 ctccacaaca gccaaaacaa caaacgcctg tacaacaaat tgctgttgga aaagaagctc
     2341 cagacttcac cttgcaatcc atggatggca aagaagtcaa gttatctgat tttaagggta
     2401 aaaaggttta cttgaagttt tgggcttcat ggtgtggtcc atgtaagaaa agtatgcctg
     2461 agttgatgga attggctgca aaaccagatc gcgatttcga aattcttagt gtcattgcac
     2521 caggaattca aggtgaaaaa actgttgagc aattccccca atggttccaa gatcaaggtt
     2581 ataaggatat tcccgttctt tatgatacta aagcaaccac cttccaagct tatcaaatta
     2641 gaagcattcc tacagaatac ctaattgata gtcaagggaa gattggaaag attcaatttg
     2701 gtgttatcag caacgcggat gcagaagcag cctttaaaga aatgaactag catagataaa
     2761 gagaatctga ttacaagatg tagtcagatt ttggtaactt ctaaaactaa cttccagttt
     2821 cccacaacct agcttttagt atgagaaaaa cgcgtgacat cagtggaaat ccgtcttaga
     2881 ctaacttcca ctggttttct ttttcttgat atataagggt tcaaaagcga aaagactcag
     2941 aaaaagtgca cgacaaatag ccctaaaaca gagtcgtctt agagtaactt ccagttttgg
     3001 gagagagatg gaagttactt tgagaagtta cggtagtcag atttttttag aaaaacattt
     3061 tataaatatt cacatatctc ctatatttat ggtaaaatag aatcatcagt ttattttgga
     3121 gtcaaagatg aatatattta gaacaaagaa tgttagttta gataaaacag agatgcatag
     3181 gcatttgaag ttatgggatt tgattttgct gggtatcgga gccatggtag ggacaggcgt
     3241 ctttacaatc acaggtactg cagctgcaac acttgctggc ccagccctag tgatttcaat
     3301 cgttatttct gccttgtgtg tgggattatc agccctcttt tttgcagaat ttgcctcgcg
     3361 agtacccgct acaggaggtg cctatagtta cctctatgct atcttaggag aattccctgc
     3421 ctggttggct ggttggttaa ccatgatgga gttcatgaca gccatatcag gcgtagcttc
     3481 gggttgggca gcttatttta aaggcttgct ctctcaatac gggatagcct ttcctcaggc
     3541 tttaaatggt acctttaatc ctcaagcagg gacatttgtt gatttattgc ctattcttgt
     3601 cttggtcttg gtgacttcgc ttgttttact aaatgccaag gcagccttac gctttaattc
     3661 gattctagtt attttgaaat tttccgcttt agctctcttt gttttagtag gaatttggaa
     3721 tattaagttt gataattgga gcaattttgc tccttacggt tttggacaaa tctatggagc
     3781 tagcactggt attatggctg gtgcgtcctt gatgttcttc ggttttttgg gatttgaatc
     3841 catctctatg gctgtagatg aagtcaagac tcctcaaaaa aatattccta gagggattgt
     3901 cttatcgctt tctatcgtaa ccattctcta tgccttggtg acccttgttt tgactggtgt
     3961 ggttcactat agtcatttaa atgtcgatga tgccgttgcc tttgcccttc gtagtgttgg
     4021 gattagttgg gcagccaact atgtgtcatt agtggctatc ttgaccttga ttacagtttg
     4081 tatttcgatg acctatgccc tatcgcgtat gatttacagt ttagcacgtg acggcttagt
     4141 gcctgctgcc tttaaggaac tgacgaagac tagcaagata ccaaagaatg ctactatctt
     4201 gacaggtcta gcttcagcag tagcagcagg aatgttccca ctagccagta ttgcagcctt
     4261 tttaaatatt tgtaccttag cctacttgat catgttggct tatggtctga ttcgcttacg
     4321 gaaagaaaaa ggaatgccca aagctgggga atttaaaaca ccattggtac ctctgttacc
     4381 aattttatcg atcatcattt gtttatcctt catgttacag tataatatga atacctggat
     4441 tgctttccta gttgcgttgt tagtaggaag tattatttac ttcacttatg gctataagca
     4501 ttctaccata gaagaataaa gtgaaagtct attgagttgt tgacactcag cagattttta
     4561 tatgtgaaag aaatttcaaa ttttttctaa aaatatcttg acagattaaa ttttcaggag
     4621 tagaatattt actagttaat taaatggtta aggagttgtt catgaagaaa caaaatttat
     4681 ttttagtcct gttaagtgtc tttcttttgt gcttgggggc ttgtggtcaa aaggaaagtc
     4741 agacaggaaa ggggatgaaa attgtgacca gtttttatcc tatctacgct atggttaagg
     4801 aagtatctgg tgacttgaat gatgttcgga tgattcagtc aagtagtggt attcactcct
     4861 ttgaaccttc ggcaaatgat atcgcagcca tctatgatgc agatgtcttt gtttaccatt
     4921 ctcatacact cgaatcttgg gcaggaagtc tggatccaaa tctaaaaaaa tccaaagtga
     4981 aggtcttaga ggcttctgag ggaatgacct tggaacgtgt ccctggacta gaggatgtgg
     5041 aagcagggga tggagttgat gaaaaaacgc tctatgaccc tcacacatgg ctagatcctg
     5101 aaaaagctgg cgaagaagct cagattatcg ctgataaact ttcagaggtg gatagtgagc
     5161 ataaagagac ttatcaaaaa aatgcgcaag cctttatcaa aaaagctcag gaattgacta
     5221 agaaattcca accaaaattt gaaaaagcga ctcagaaaac atttgtaaca caacatacag
     5281 ccttttctta tctagcgaag agatttgggc ttaatcaact tggtattgca ggtatctctc
     5341 ctgaacaaga accaagtcca cgacaactaa cagaaattca ggaatttgtt aagacctata
     5401 aggttaaaac gatttttaca gaaagtaacg cttcttcaaa agtagctgaa actcttgtca
     5461 aatcaacagg tgtgggtctt aaaactctga atcctttaga gtcagaccca caaaatgaca
     5521 agacctattt agaaaatctt gaagaaaata tgagtattct agcagaagaa ttaaagtgag
     5581 gaaagaatga aaatcaataa aaaatatcta gcaggttcag tggcagtcct ttccctaagt
     5641 gtttgttcct atgaacttgg tcgtcaccaa gctggtcagg ttaagaaaga gtctaatcga
     5701 gtttcttata tagatggtga tcaggctggt caaaaggcag aaaatttgac accagatgaa
     5761 gtcagtaaga gagaggggat caacgccgaa caaattgtta tcaagattac ggatcaaggt
     5821 tatgtgacct ctcatggaga ccattatcat tactataatg gcaaggttcc ttatgatgcc
     5881 atcatcagtg aagaacttct catgaaagat ccgaattatc agttgaagga ttcagacatt
     5941 gtcaatgaaa tcaagggtgg ctatgtgatt aaggtagacg gaaaatacta tgtttacctt
     6001 aaagatgcgg cccatgcgga caatattcgg acaaaagaag agattaaacg tcagaagcag
     6061 gaacgcagtc ataatcacaa ctcaagagca gataatgctg ttgctgcagc cagagcccaa
     6121 ggacgttata caacggatga tgggtatatc ttcaatgctt ctgatatcat tgaagatact
     6181 ggtgatgctt atatcgttcc tcacggcgac cattaccatt acattcctaa gagtgatttg
     6241 tctgccagtg aattagctgc tgcccaagcc tattggaatg ggaagcaggg atctcgtcct
     6301 tcttcaagtt ctagtcataa tgcaaatcca gctcaaccaa gattgtcaga gaaccacaat
     6361 ctgactgtca ctccaactta tcatcaaaat caaggggaaa acatttcaag ccttttacgt
     6421 gaattgtatg ctaaaccctt atcagaacgc catgtagaat ctgatggcct tattttcgac
     6481 ccagcgcaaa tcacaagtcg aaccgtcaga ggtgtagctg tccctcatgg taaccattac
     6541 cactttatcc cttatgaaca aatgtctgaa ttggaaaaac gaattgctcg tattattccc
     6601 cttcgttatc gttcaaacca ttgggtacca gattcaagac cagaacaacc aagtccacaa
     6661 tcgactccgg aacctagtcc aagtccgcaa cctgcaccaa atcctcaacc agctccaagc
     6721 aatccaattg atgagaaatt ggtcaaagaa gctgttcgaa aagtaggcga tggttatgtc
     6781 tttgaggaga atggagtttc tcgttatatc ccagccaagg atctttcagc agaaacagca
     6841 gcaggcattg atagcaaact ggccaagcag gaaagtttat ctcataagct aggagctaag
     6901 aaaattgacc tcccatctag tgatcgagaa ttttacaata aggcttatga cttactagca
     6961 agaattcacc aagatttact tgataataaa ggtcgacaag ttgattttga ggctttggat
     7021 aacctgttgg aacgactcaa ggatgtccca agtgataaag tcaagttagt ggatgatatt
     7081 cttgccttct tagctccgat tcgtcatcca gaacgtttag gaaaaccaaa tgcgcaaatt
     7141 acctacactg atgatgagat tcaagtagcc aagttggcag gcaagtacac aacagaagac
     7201 ggttatatct ttgatcctcg tgatataacc agtgatgagg gggatgccta tgtaactcca
     7261 catatgaccc atagccactg gattaaaaaa gatagtttgt ctgaagctga gagagcggca
     7321 gcccaggctt atgctaaaga gaaaggtttg acccctcctt cgacagacca tcaggattca
     7381 ggaaatactg aggcaaaagg agcagaagct atctacaacc gcgtgaaagc agctaagaag
     7441 gtgccacttg atcgtatgcc ttacaatctt caatatactg tagaagtcaa aaacggtagt
     7501 ttaatcatac ctcattatga ccattaccat aacatcaaat ttgagtggtt tgacgaaggc
     7561 ctttatgagg cacctaaggg gtatactctt gaggatcttt tggcgactgt caagtactat
     7621 gtcgaacatc caaacgaacg tccgcattca gataatggtt ttggtaacgc tagcgaccat
     7681 gttcaaagaa acaaaaatgg tcaagctgat accaatcaaa cggaaaaacc aaacgaggag
     7741 aaacctcaga cagaaaaacc tgaggaagat aaggaacatg atgaagtaag tgagccaact
     7801 caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc agataatctt
     7861 tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac tacagatgag
     7921 gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga tgcggaggcc
     7981 ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac attgactggt
     8041 ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc agaagtagat
     8101 agtctcttgg ctttgttaaa aaaaagtcaa ccggctccga taccgtagta aaatgaatgg
     8161 agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa aaacgtaaca
     8221 gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta aatggttaaa
     8281 taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac ttcatcattt
     8341 attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga tcagctgtta
     8401 tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag gaaaataagg
     8461 acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt gaaaacttga
     8521 caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta atcaaaatta
     8581 cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat gggaaagttc
     8641 cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat caacttaaag
     8701 acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat ggaaaatatt
     8761 atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat gaaatcaatc
     8821 gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt gctgtagcaa
     8881 ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct gatattatcg
     8941 aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac attcccaaaa
     9001 gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga aaaaatatgc
     9061 aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa tctgtagcaa
     9121 aaggatcaat tagcaagcca gcaaataaat ctgaaaatct ccagagtctt ttgaaggaac
     9181 tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc tttgaccctg
     9241 ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac cattaccact
     9301 ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg gtgcctatca
     9361 gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg tctagtctag
     9421 gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct tcagcatctg
     9481 atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct tatattgtaa
     9541 gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa ccgactcttc
     9601 caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga acttcacatg
     9661 agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct gaagatgaat
     9721 caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag gacttgacag
     9781 aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt cataatggat
     9841 tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa atgaaagatt
     9901 tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt gtcaaacgtg
     9961 aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga gatcaccatc
    10021 atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac agtaactatg
    10081 aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt tatactggag
    10141 aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat caaaacttta
    10201 ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg gagaaaaaat
    10261 taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg gagaaagtat
    10321 ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta gatcaacctt
    10381 atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca gaagtaagtt
    10441 atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt caaacgattt
    10501 tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt gcagtgccta
    10561 aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct tatttagaaa
    10621 ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa ggaacaacca
    10681 gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg gacaatcaat
    10741 cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat aaaccaagta
    10801 ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat gaaaaggtag
    10861 aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact gggaatagta
    10921 ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa aaagtagcaa
    10981 aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg gacggaacaa
    11041 ttgaattata tttaccatca ggagaagtca ttaaaaagaa tatggcagat tttacaggag
    11101 aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta tctactggaa
    11161 cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag gcaccaaaca
    11221 aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca gaagggaatg
    11281 tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta gatcctgtac
    11341 aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt gttatattca
    11401 atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa aagaatttat
    11461 ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat gaagttctct
    11521 caaaagt
//
LOCUS       DAYYQN010000059        11211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYYQN010000059 DAYYQN010000000
VERSION     DAYYQN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.060"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            123..503
                     /locus_tag="ACHBRQ_001673"
     CDS             123..503
                     /locus_tag="ACHBRQ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593298.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            697..987
                     /locus_tag="ACHBRQ_001674"
     CDS             697..987
                     /locus_tag="ACHBRQ_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593299.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            1038..1730
                     /locus_tag="ACHBRQ_001675"
     CDS             1038..1730
                     /locus_tag="ACHBRQ_001675"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HFM9593300.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1783..4194
                     /locus_tag="ACHBRQ_001676"
     CDS             1783..4194
                     /locus_tag="ACHBRQ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HFM9593301.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     CEAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4444..5145
                     /locus_tag="ACHBRQ_001677"
     CDS             4444..5145
                     /locus_tag="ACHBRQ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9593302.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5147..7855
                     /locus_tag="ACHBRQ_001678"
     CDS             5147..7855
                     /locus_tag="ACHBRQ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9593303.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7951..8442)
                     /gene="tpx"
                     /locus_tag="ACHBRQ_001679"
     CDS             complement(7951..8442)
                     /gene="tpx"
                     /locus_tag="ACHBRQ_001679"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFM9593304.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8565..9494)
                     /gene="psaA"
                     /locus_tag="ACHBRQ_001680"
     CDS             complement(8565..9494)
                     /gene="psaA"
                     /locus_tag="ACHBRQ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HFM9593305.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9526..10374)
                     /locus_tag="ACHBRQ_001681"
     CDS             complement(9526..10374)
                     /locus_tag="ACHBRQ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9593306.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10371..11093)
                     /locus_tag="ACHBRQ_001682"
     CDS             complement(10371..11093)
                     /locus_tag="ACHBRQ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9593307.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3301 a         2069 c         2544 g         3297 t
ORIGIN      
        1 gaaaagtatt atattttatt tcatattata caaattttta ttttataata tcagaaaata
       61 ctttttttaa aagcaaatat gatacaattt tatttgaaaa aaataaaaaa ggagatttta
      121 ttatgaaatt aaaaacactt gctttaatta gtggtatcgt cggtcttgtg ggaggaattt
      181 tacttcttat tggtcctttt gtcttgttgg gaacagcggt aaacacagtt gctacaactc
      241 ttaatggagg agctactgca ggggcttttt caggtgttgc cttactcttg aatgccttga
      301 agattgcaaa tcttgttctt ggtatcattg ctattgttta ctataaagga gataatcgtg
      361 taggtgcagc tccgtctgta ctaatgattg tttttggtgg agttagtctc attccattct
      421 taggatgggt tggggggatt cttgttatta tcggaggatc tctattcctt gcaacattga
      481 agaaattcaa atcagaagaa taaaaggtat tttagcatga aaagaacaaa aaagtttatc
      541 ggtataggag tagctctatt atctcttttt cttctagttg catgtggaac ataaagttca
      601 aaggatactt caacaagtaa tgatgagaag acagtagcaa catccaatag ttcaaaagaa
      661 acaatcactt tcgatacacc ggttgtaaca gacgatgtga ttgaatcaat acgcacttat
      721 gcagattata tagatcttta taaaaatatt tttgatgatt attttactaa agctgaggaa
      781 ggtttcaaag gcacagctat ggaaaataat gactcgttta ctaaactaaa agagtcaact
      841 caaaaattat tcgatgcgca gaaaaaaagg ttaaataatg aagatagaat agaaacaact
      901 aaaaacaatg tgattgccaa acattgtcaa acagtccttt cctttttggt tttgactagc
      961 ttttttgtga aaaattgtgt aaaatagaat agataaacga ggggaaacct cggaaaattt
     1021 aaaggagaat ccatctaatg gtaaaattgg tttttgctcg ccacggtgag tctgaatgga
     1081 acaaagctaa ccttttcact ggttgggctg atgttgattt gtctgaaaaa ggtacacaac
     1141 aagcgattga cgctggtaaa ttgatcaaag aagctggtat caaatttgac caagcttaca
     1201 cttcagtatt gaaacgtgct atcaaaacaa ctaacttggc tcttgaagct tctgaccaat
     1261 tgtgggttcc agttgaaaaa tcatggcgct tgaacgaacg tcactacggt ggtttgactg
     1321 gtaaaaataa agctgaagct gctgaacaat ttggtgatga gcaagttcac atctggcgtc
     1381 gttcatacga tgtattgcct ccaaatatgg accgtgatga tgagcactca gctcacacag
     1441 accgtcgtta tgcttcactt gacgactcag taatcccaga tgctgaaaac ttgaaagtga
     1501 ctttggaacg tgctcttcca ttctgggaag ataaaatcgc tccagctctt aaagatggta
     1561 aaaacgtatt cgtaggagct cacggtaact caatccgtgc ccttgtaaaa cacatcaaag
     1621 gtttgtcaga tgacgagatc atggacgtgg aaatccctaa cttcccacca ttggtattcg
     1681 aattcgacga aaaattgaac gtcgtttctg aatactacct tggaaaataa aaaattgtaa
     1741 gtctagaatt gatttctagg ctttttatgt tagtatggaa gtatgataag gaataaaaaa
     1801 caagattatg tactggccta caagcaacca gtttcaacca cttacatggg ttgggaagaa
     1861 gaagctttac cgataggcaa tggttcttta ggagcaaaag tatttggcct tataggggct
     1921 gaacggattc aatttaatga aaaaagtctc tggtctggag gtccacttcc tgatagttca
     1981 gattatcagg gtggaaatct tcaggatcag tatgtttttt tagctgagat tcggcaggct
     2041 ttggagaaga gagattacaa tctggctaag gaactggctg agcagcacct aattgggcca
     2101 aaaacgagtc aatatgggac ctatctgtct tttggggata ttcacattga gttcagccag
     2161 caaggtacga ctttgtctca ggtgacggac tatcagagac agctgaatat tagtaaggca
     2221 cttgtgacga cttcttatgt ctataaggga acgcgatttg aatgtgaagc ttttgcgagt
     2281 tttccagatg atctcttggt tcagcgtttt actaaggaag gggcggaaac tctagatttt
     2341 actatagaac tatccttgac ctgtgatttg gcttctgatg gaaagtatga gcaggaaaaa
     2401 tctgattaca aggagtgtaa gttggatatt actgattctc atatcttgat gaagggaaga
     2461 gttaaggata atgatctgcg gtttgctagt tatctagctt gggaaacgga tggagatatt
     2521 agagtttggt cagatagggt tcagatatca ggagccagtt atgccaatct cttcttggcc
     2581 gctaagacgg attttgccca aaatcctgct agcaattatc gcaagaaact agatttagag
     2641 caacaggtga tagacttggt ggacacagct aaagaaaagg gctataccca attgaaatca
     2701 aggcatatcg aggactacca agccttattc cagcgtgttc aattggattt ggaagctaat
     2761 gttgacgcat tcactacaga tgatttgtta aaaaattata agccacaaga agggcaggct
     2821 ttggaggagc tgttcttcca gtatggacgg tatttattga ttagttcgtc cagagactgc
     2881 ccagatgctc taccagctaa cctacaggga gtctggaatg cggtcgacaa tcctccttgg
     2941 aattcggact atcacttaaa tgtcaatctg cagatgaatt attggccagc ctatgttacc
     3001 aatctcctag aggcggtctt tccggtcatc aactatgtag atgatttgcg tgtctatggt
     3061 cgtctagcgg ctgtaaagta tgcaggaatc gtctctcaaa aaggtgagga aaatggttgg
     3121 ttggttcata ctcaagcgac tccctttggt tggacggcac ctggttggga ttactattgg
     3181 ggttggtcac cagctgccaa tgcgtggatg atgcaaactg tttatgaagc ctattcattt
     3241 tatagggacc aagactatct cagggagaaa atttatccca tgttgaggga aacggttcgt
     3301 ttttggaatg cctttttaca taaggatcag caggcgcagc gttgggtgtc ttctccgtct
     3361 tattccccag aacatgggcc gatttcgatt ggcaatactt atgaccaatc tctgatttgg
     3421 cagttatttc atgattttat tcaggctgct caggaattgg gactggatga ggacttgttg
     3481 actgaggtta aggagaagtc tgatttacta aatcctttgc aaatcactca atctggtcga
     3541 atcagggagt ggtatgagga ggaagagcag tattttcaaa atgagaaagt ggaggcccag
     3601 catcggcacg cttcccatct agtgggactc tatcctggca atctctttag ctacaaggga
     3661 caagagtata ttgaagcggc acgtgctagc ctcaatgatc gtggagatgg cggcacaggc
     3721 tggtccaagg ctaataagat caatctctgg gcgcgtttgg gagatggcaa tcgagcccat
     3781 aaattattgg cagagcagtt aaaaacatcc accttgccaa atctttggtg tagccatcct
     3841 ccttttcaga tagatggtaa ttttggtgct actagtggca tggcagaaat gttactccag
     3901 tctcatgcag cttatctggt acctctagct gccctacctg atgcttggtc aacaggttct
     3961 gtttcaggct taatggcacg tggacatttt gaagtgagca tgagctggga agataaaaaa
     4021 ctcttacagt tgaccatttt atcaaggagt ggaggagatt tgcgagtttc ttatccagat
     4081 attgagaaga gtgtgattaa aatgaatcaa gaaaaaataa aagcgaaatg catggggaaa
     4141 gattgtattt cggtggcaac agcagaaggt gatcttgttc aattttattt ttaagaagat
     4201 gttataaggc agtaatttga aacttccttt taataaggat ttaagaatat aggcagtttc
     4261 caactagttg aaaaagcgtt ataatgataa taggaagtaa tactcaatga aaatcaaaga
     4321 gcacaaacta ggaagctagc cgcaggttgc tcaaaacagt gttttgaggt tgtagatgga
     4381 agctgacgtg gtttgaagag agattttcga ggagtataat ttgtttgata gagggtgggt
     4441 ctgatggctt atattgagat gaaacactgt tacaagcgtt atcaggttgg ggacacggag
     4501 attgtggcca atcgtgatgt gaattttgag attgaaaagg gggagctggt tattatcctt
     4561 ggtgcttcag gtgcaggcaa gtcaacagtt cttaaccttc ttgggggaat ggataccaat
     4621 gatgaagggg aaatctggat tgatggtgtt aatattgcgg attatagttc ccaccagcgc
     4681 accaattacc gtagaaatga tgtggggttt gtttttcagt tttataatct agtttctaat
     4741 ctgacagcta aggaaaatgt ggaactggct tctgaaattg tgacagatgc cttgaatcct
     4801 gatcaggtct tgacagatgt aggtctggct catcgtctca ataactttcc agcccagctt
     4861 tctggagggg agcaacagcg agtctccatt gcacgcgcgg tagccaaaaa tcctaaaatt
     4921 ctcctttgtg atgaaccgac tggagccttg gattatcaga cgggcaagca ggttttgaaa
     4981 attctccaag acatgtctcg tcaaaaggga gcgacggtga tcatcgtgac tcataatgga
     5041 gctttggcgc ccattgctga tcgcgtgatt catatgcacg atgccagtgt caaggatgtg
     5101 gtgctcaacc agcatcctca ggatattgac agtttggagt actagcatga tcaagcgaaa
     5161 aacttattgg aaggacttaa ttcagtcctt cacagactcc aaggggcgtt ttttatccat
     5221 cttgatcctg atgatgttgg gatctctagc cttagtaggc ctcaaagtaa ccagtcccaa
     5281 catggaggcg acagctaatg cttatttaac aactgctcaa accttggatt tggcagtcat
     5341 gtctaactat ggcttggatc aagcagacca agaagaacta aaacagacgg agggcgcaga
     5401 ggtcgagttt ggctatttga cagatgtgac tatggataat gggcaggatg ccattcgact
     5461 gtactccaaa ccagagcgaa tttcaacctt tcagctaaga aagggacgac ttcctcagtc
     5521 agacagggaa atcgctttgg ccactcattt gcaaggccaa tacagcgtgg gacaggagat
     5581 tagttttaaa gaaaaagaag agggtcattc ctctttaaaa gaccatactt ataccattac
     5641 tggttttgtg gattcggctg aaatcctctc ccagcgagat atgggctacg caggaagtgg
     5701 aagtgggact ctgacagcct atggggtgat tttacctagt caatttgatc agaaagtcta
     5761 caatatagct cgtttgaaat atcaagattt agcaggttta aatgcctttt catcagctta
     5821 tgaagaaaaa tccaagcaac atcaggaaga ccttgaacaa actttatcag ataatggcaa
     5881 ggtacgtctg caacttttga aaaaagaagg acaagagtct ctagacaagg ggcaagagac
     5941 tcttgacaag gctcagacta atttgcagga aggcaagcgt cgtttagcag ctgctcaagc
     6001 tcgtatacag gctcaagaaa gtcaactagc cttgtttcct caagctcaga gagagcaggc
     6061 tagtgctcaa cttacccaag ccaagcagga actgggcaag gaagaggaca aactaaagca
     6121 agctgaacaa aatctagccc aagaaaagga aaaattagaa aaacatcagc aagtcttgga
     6181 tgatttggcg gagccaaggt atcaggttta taatcgtcag accatgccag gtggtcaggg
     6241 ctatcttatg tatagcaatg cttcatccag tattcgagca gtgggcaata tctttcctgt
     6301 ggtactttat gccgtagcag ccatggtgac ctttacgacc atgactcgct ttgtagacga
     6361 agagcgaact catgcaggga tttttaaggc cttgggttat cgtagtaagg atattatcgc
     6421 caagtttctc ctttatggac tagtagctgg gactgtcgga acggctctag gtagtatact
     6481 tggtcattat ttgctagcca gtgtaatttc aagtgtcatt acaaaaggca tggtggtggg
     6541 agaaacccag attcagttct attggaccta tagcttacta gctcttgtct tgagctggtt
     6601 ggcgagtgtg ttaccagcct atctggtggc ttggagggaa cttcatgacg aagcagccca
     6661 gcttctactt cctaaacctc ctgtcaaagg agctaaaatc ttattggagc gtatcggttt
     6721 tatctggcgt cgtctcagtt ttactcataa ggtaacagcc cgcaacatct ttcgttataa
     6781 gcagagaatg ttgatgacaa tctttggtgt ggcaggttct gtagctctgc tctttgcagg
     6841 tttgggaatc caatcttctg tagcaggagt tccgtctaaa cagtttcaac aaatccaaca
     6901 gtatcagatg cttgtctctg aaaatcctag tgcgaccaat caggacaagg tagagctagc
     6961 agaagtgttg aaagggcagg agatactagc ctaccagaaa atctattcta aaacgctaga
     7021 caaggatttc aagggcaggg ctggtcttca aaacattact cttatgatga tagagaagga
     7081 agatttgact ccctttatcc atcttcaaca tcatcagcag gagctgacat taaaagatgg
     7141 catcgttatt acagctaaac tcgcccagct ggcaggtgtc aaggttgggc agactttaga
     7201 aattgaaggt aaggaactaa aggtcgctgc tattactgag aactacgttg gtcactttat
     7261 ttatatgagt caggctagct atgagcaact ttacggacag ctaccccaag ccaacactta
     7321 tctggtctca ttaagggata ccagtgcaac tagtatcgaa agtcaggcgg gcttgcttat
     7381 gaatcaatct gcggtgtcca gtgttgtcca aaatgcttca gccattcgac tcttcgactc
     7441 tatcgctagc tcactcaatc agaccatgac catcttggtc atcgtatcgg ttctattagc
     7501 tattgtcatc ctttacaatc tgaccaatat caacgtagct gagagaatcc gtgaactctc
     7561 cactatcaag gttctcggtt ttcataataa tgaagtcacc ctctacattt accgtgagac
     7621 gattgtgctg tcccttgtgg gaatcgtact tggtctggta gctggtttct atttacacca
     7681 atttttgatt caaatgattt cgcctgcgac tattctcttt tatccgcagg taggctggga
     7741 agtctatgta atcccagtgg cagcagtaag catcattttg accttgcttg gtttcttcgt
     7801 caattattat ctgagaaagg ttgacatgtt agaagccctg aaatctgtag agtaaggtag
     7861 ttatttttag ctgattgaac ttctatttac taatattcaa aaatcctccg tttcaaagag
     7921 cagggaactc tttgtgacag aggatttttt ctatagggct ttagcagctg caattgcggc
     7981 ttcgaagttt ggctcagaat tgatattatc cacgtattca acgtagcgaa tcgtattgtc
     8041 agtatcgagg acaaagactg cgcgtgctaa taggtgccat tcgttgatca agagggcata
     8101 atcgcgtccg aaagaatggt caaagtagtc tgaaagcata atggcattgt caaggccttc
     8161 agcaccgcac caacgttttt gagcaaaagg taggtccatt gaaacagtca atacgaccgt
     8221 gttgtccagt ccagccaatt cttcattaaa acgacgtgtt tgagttgagc agatgcctgt
     8281 atcgatagaa ggaacgacac tcaagacttt tttcttgcca tcaaaatcag ccagagattt
     8341 tttagaaaga tctgttgtag taagagaaaa atcaagcgcc ttgtcgccga cttgtagttg
     8401 tttacctgta aagctcacag gatttccgag aaaagttacc ataggatact ccaatctttt
     8461 ttcttccatt gtatctgaaa cagtcagaat tttccaatga tttgaccgga aatgtgtgca
     8521 tagaaaaaac gccagctcac atgagaatga cgtttttcag aggcttattt tgccaatcct
     8581 tcagcaatct tgtcaaggtt gtatttcatc atgctgtagt agctgtcgcc ttctttacct
     8641 tgttctgcga tagagtcagt aaagatttgt gcgtagattg ggatgtttgt gtcttgagaa
     8701 acagttttca ttggacggtc atccacactt gattctacaa agagtgatgg aacttttgtt
     8761 tggcgaagtt tttcaaccaa ggtcttgatt tgttcaggag ttccttcttc ttcagtattg
     8821 atttcccaga tgtaggcact tgggacacca taggctttag agaagtattt gaatgctcct
     8881 tcgctggtta caatgagttt cttttcagca gggatcttat taaatttatc cttactttct
     8941 ttatcaagtt tgtctaactt atcagtatat tctttgagat ttttttcata gaattcttta
     9001 ttgttagggt ctttggcgct caattgtttg gcgatatttt tagcaaaaat aataccgttt
     9061 tcaaggttaa gccaagcgtg tgggtcttct tttccttttt cattttgacc ttcaaggtag
     9121 ataacatcaa cgccgtcgct gactgcgaag tagtctttgt tttcagtttt cttggcattt
     9181 tctaccaatt ttgtaaacca agcattgcca cctgtttcaa ggttgatacc gttatagaaa
     9241 atcaaatcag cctcagaagt tttcttaacg tcttcaggaa gtggttcgta ttcgtgtggg
     9301 tcttgcccaa tcggaacgat actatgaagg tcaattttgt caccagcaat atttttagta
     9361 atatcagcga tgattgagtt tgtagcaaca acttttagtt tttgaccaga agttgtatct
     9421 ttttttccgc tagcacatgc tacaagaatg attgcagaaa gaaagagaac gagtaatgta
     9481 cctaattttt tcattagatc ctccaattta ttagggcttt gccccttatt ttaacaaatg
     9541 tttatttttc agtttcaaat atcgttgttt gggagcgata aagaagctaa tgagaaagaa
     9601 actagcagct gtaagcacga tactagaacc tgccgcaaca ttaaaactat agccaataaa
     9661 gagtcccaaa actgaagcag tagctccgaa ggttgaggaa aggaaaatca tgcttttcag
     9721 actattagca tacagataag cagttgcagc tggggtaatc agcatggcta caatcaggat
     9781 agttccgaca ctttgcatgg ctgtcacaga cacgagagtc aggagtacca tgagaaggta
     9841 gtgatagaaa ttgacaggca ttcccatggc tttagccaag agttcatcaa aggaagttat
     9901 caagagttgc ttgaagaaaa tccagattaa caagaggatg gctgccccca cacccatagt
     9961 aataaacata tccgtatctt ggacggccag aatattacca aaaaggatat ggaaaaggtc
    10021 agttgaactt ttagcgacac caatcaagat gataccgagg gctaagaaag aagaaaaggt
    10081 aatgccgatg gcggtatcgc ttttgataat cgagtttcct ttgatgtagg taatgatgat
    10141 ggcagctagc aatccaaaga caatggctcc gataaagaag tcaaggccca agatgaagga
    10201 gagggctaca cctggtaaga cagcatgtga aatggcatct cccatgagtg acatcccgcg
    10261 tagaataatg aaacatccca cggctccagc tacaatcccg acgacaatag ctgttatcaa
    10321 ggcattttgt aggaaatgga atttttgcaa tccatcgata aattctgcaa tcataggtca
    10381 cctccattga aaaagagttg attaccgtaa gcttctttta gattggtttc ggtaaaagtt
    10441 tcttttgttg gaccaaaggc aatcacttct cgattgacaa gtaagacttg atcgaagtag
    10501 tggggaatct tgctgaggtc gtggtgaacg atgagaaccg tcttcccagc ttttttcaaa
    10561 tctctcagcg tattcatgat gatttcctca ctgacagagt caatcccagc aaagggttca
    10621 tccaagagga tatagtcggc ttcctgcacc aaacatctgg caatcaagac ccgctggaat
    10681 tgacctccag acagttgact aatttgacgt tcagcgtagt cagctaggcc gacgatttca
    10741 agggcctctt gcactttctt ccaatgttta gcctttaaac ttcgaaagag aggaatagag
    10801 ggaaatagtc ctaacgagac gcattccttg accttgatgg gaaagttgta gtcgatattg
    10861 attttttgtt cgacataggc aattcggtgt aaggattttt taacttcctt gtcatcgaga
    10921 aatgcctgac cttgatgtgg gataattccc agcatacctt ttaatagtgt tgatttccca
    10981 gcgccgtttg gaccaatgat gccggtaatt gttggtccat ggagcactag tgaaatatcc
    11041 ttaagtgcca acgtttcttt gtaggagaca ctgaggtttt cgatacgtat cataaacttg
    11101 tattcctcct gtctcttaat atacattaaa aaaaaaatta agtcaagtta atttttgaaa
    11161 aaattaaaat aataactgaa aaatagattc taaagataac tttcaggata a
//
LOCUS       DAYYQN010000060        10743 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYYQN010000060 DAYYQN010000000
VERSION     DAYYQN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.061"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(105..1529)
                     /locus_tag="ACHBRQ_001683"
     CDS             complement(105..1529)
                     /locus_tag="ACHBRQ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9593308.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAIEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="ACHBRQ_001684"
     CDS             complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="ACHBRQ_001684"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HFM9593309.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4472..6430)
                     /gene="ligA"
                     /locus_tag="ACHBRQ_001685"
     CDS             complement(4472..6430)
                     /gene="ligA"
                     /locus_tag="ACHBRQ_001685"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9593310.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6523..7701)
                     /locus_tag="ACHBRQ_001686"
     CDS             complement(6523..7701)
                     /locus_tag="ACHBRQ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9593311.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7781..8644)
                     /locus_tag="ACHBRQ_001687"
     CDS             complement(7781..8644)
                     /locus_tag="ACHBRQ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HFM9593312.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8647..10548)
                     /locus_tag="ACHBRQ_001688"
     CDS             complement(8647..10548)
                     /locus_tag="ACHBRQ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9593313.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3231 a         2362 c         1906 g         3244 t
ORIGIN      
        1 gtttcaatct attataatac caaaaagttc gtattttttc tataattatt attattttat
       61 agcaaaaaaa taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa
      121 acaatggatt tgacatttgt catcgcttca atgctatatt taattccttg cactccagct
      181 ccagaacctt tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta
      241 atgtggactg tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt
      301 gtaaagactg atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct
      361 acactagcca cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc
      421 atatcttttg taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt
      481 ggtgtaagag cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg
      541 gctgaagcat tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt
      601 aatttagaaa cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca
      661 atgacacgtt taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt
      721 tgcttggcag catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca
      781 agttccaaca tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt
      841 gaacctgtaa agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct
      901 gaaccacgac ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct
      961 ttagccaaca agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc
     1021 cctgcaatca aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga
     1081 gcaatcgcta gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt
     1141 gctgcctcaa aaccaccacc ttctattgct tgtccagtga tacggagacc ttcctcagca
     1201 gcataacgaa tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt
     1261 gctacttctt tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct
     1321 gttttatgta aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct
     1381 gcacgcgcag cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc
     1441 aattcttctt gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt
     1501 ccatttacta aattctgata tcttgtcaaa ttcttgtcct cctgaaaatg attaagatat
     1561 attttaccaa attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca
     1621 caaacttgat ttttcaaagt atactcaata aaaataaaaa aaatccacta cgtcaaggcg
     1681 aggctaatgt ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa
     1741 taaaaaaact gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa
     1801 acaagggtgt gctactccaa tttcagccta ctataactgt catagattgc tgaaacaaag
     1861 tctaggtaaa agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag
     1921 gaggtcttgt tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg
     1981 ttctttctat tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat
     2041 ttgtctgtgt aaatatagcg agatagatgg tatttataaa acactcaaga cagctagact
     2101 aatatcattt aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt
     2161 tcctgcattt tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc
     2221 tttgccgtga acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac
     2281 atgatggtaa atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg
     2341 gatgacttga cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac
     2401 ttggttcaca ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt
     2461 caactcacca ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc
     2521 cataaaggcc atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat
     2581 gatttgctct gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt
     2641 gtcatgggct tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg
     2701 acttcctagg attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga
     2761 cttgatagca ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa
     2821 accaatattt ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc
     2881 tgttcccata tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct
     2941 ttgacgaatc atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc
     3001 gtcaatatta tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat
     3061 ttcgtgttca ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg
     3121 attcatacga taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa
     3181 ggtatggtta tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac
     3241 catcaccttc aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt
     3301 ttctggcgcg ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc
     3361 atcgtattct ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt
     3421 cttgatgtaa tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc
     3481 acccaagaaa gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc
     3541 acgaatatgc atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc
     3601 cgaaccgtgc ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga
     3661 acgtttgcca tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg
     3721 agggaattgg acttgatact gataagcctt acctaccaaa tcttcctcaa catccaaact
     3781 ccagacaccg attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc
     3841 aaaagtcttc caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc
     3901 tgtcgctgta ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa
     3961 ttctccttgg taaccccaat gatggtcaaa actagcactg ttaatggcct tatcaaaggc
     4021 aaaaggattt tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat
     4081 cctatcatcg ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat
     4141 agaatattct ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg
     4201 tgaacttggg tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa
     4261 atcaattcgt tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg
     4321 atggtaatga atgcgcatag ggtagttata cattttttat tttttatttt tcctttttac
     4381 tttgtttcta tttcactaat aaatttttgt caatctcgtc tcaattaaca gacatagtca
     4441 tattctctaa actctgtttt taaacgatcc attacaaact ttctagccat gcctcatctc
     4501 tgacctggat accaagttct tgtgcttttt gcagtttact tccagcgtct gcacctacca
     4561 cgacgaggtc ggtcttttta gaaatactac ctgtcacttt ggcacccaga ctttcgagtt
     4621 tacttttagc ttctgagcgc ttgagtcgtt ccaattttcc tgtcaatacc acggtcaaac
     4681 ctgacaaggc cgcatccgct actaccgtct gtcctttata gtccagattg accccagttt
     4741 ctttcaattc tctgagcaga atttcagagc cttctgtcgc aaaataagtc tgaagacttt
     4801 tggcaatcac gccacctaga ctttcaatac tagccacttc ctctgaatct gcctgagaca
     4861 gattttcaat tgaatggaaa tattgaagta aaagctgact aaccttgctt ccgacatgac
     4921 gaattcccaa accaaataag agcttctcgg cagaattttc ctttgatgct tggatagcct
     4981 gatacagttt agcagcggac ttttccttaa ccccctctaa aaggaggaaa tcctcttctt
     5041 gcaaacgata aatatccgcc acatccttga ctaaattagc agcaaaaagc ttctcaacaa
     5101 tagatggacc aaggcctgta atattcatag catcacgaga agcaaagtga atcaagcctt
     5161 ccatgatttg agcagggcaa cgcggattga tacaacgtag ggccacttca tcttcaaagt
     5221 gcaacaagtc agagttacaa cttggacagt ttgtagggat atctagtttt tcttcagaaa
     5281 cccgtttgga ctctaccaca cgtaaaacgg cagggatgat gtcaccagcc ttatatacaa
     5341 tgaccgtatc gtcttttcgg atatcttttt cagcaatata atctacattg tgcagggtcg
     5401 cacggctaac agtcgtaccg gcaagttgta ctggtgttag attagcagtt ggagttacaa
     5461 caccggtacg gccaactgtc cagtcaactg ataagagttg agcttctttt tcttcggcag
     5521 ggaacttgta ggctactgcc cactttggag ccttaactgt aaaaccaagt tcttcttgac
     5581 ttgctaggtc gttgaccttg attaccactc catcaatatc gtaaggcaga ttttcccgtt
     5641 cctgtcctac ttcttggata aaattccaga tttcatctat gttttcagcc aagattcgct
     5701 taggattgac cacaaaacct agttgttcta ggtacttcaa acccttttct tggctatcac
     5761 gagttgaagg gctggcttct tgatagagaa acgttgcaag attacgcttg gcaactactg
     5821 ctgtatccaa ctgacgcaga gttcctgctg ccgcattacg aggattagca aattcaggct
     5881 ctccattttc ttggcgcact tggttaactt ggtcaaagga agcgcgtggc atgtaacatt
     5941 ccccacgaac tgtgatatct agttcttctg gcaaagtcaa agggatgtcc ttaacacgct
     6001 tgaggttttc tgtgatattt tcaccaattg aaccatctcc acgtgttacc ccagcaacca
     6061 aaatcccctt ttcataagtc agcgagatag ataagccatc gattttcagc tcacaaatat
     6121 aggtcggatg agccacttcc ttacgaacac gcgcatcaaa agcatctagc tcctcacgtg
     6181 aaaaagcatc ctgcaaacta taaagaggat actgatgact gtatttttca aaaccatcta
     6241 aaaccttgcc accaacacga tgagtcggac tgtctgctag cacttgctct ggataagcag
     6301 tttctaactc gaccaactca cggtaaaggc ggtcatactc actgtctgca accgagggat
     6361 tatcgctggt atagtactca gtcgcatagc gattgagcaa agcgactaac tcattcattc
     6421 ttttattcat aagaccattt taccataaaa caagccctcc tcacaaacga gaagggcgga
     6481 aaaaacactt agtttgaaat tatttttgaa actcaagcaa ccttatatca atttttcaaa
     6541 atgagttcga acataaataa acgatataca agacaagatg ataacaccac ttccaattat
     6601 caggaaagaa gagagatgta cacttggcaa gactgtcata aatccttttg caataggcat
     6661 aaatagaata gctaaggtaa aaattgtact cagtactctt ccaagaaatt cgctctcaac
     6721 cttggtttgt acttgagtaa aaaagtgaat attaaaaatc gtcataaaca attcacaaac
     6781 taaatttcca gaaaaggaaa gaaaagttgg aagtggtaat cccatcataa aaactccgac
     6841 acctgtcaaa gccagtaaaa tcaaaagatt ataaatatta gctttaattt tactagctag
     6901 aagagcccca atgatggaac caatagcccc catagttaaa atacttgcat aggctccttc
     6961 tgacccgtaa agctgattcg aaaagggaag tagaaattca aaagctgcaa aaaagaaatt
     7021 aacgctggaa gctaccagca aaaggaagaa aatttcttgc tgatgccaga tatagtgtaa
     7081 cccatccttg atatctacaa aaatatctct cccagtaaaa gcctttttct cttgaacttt
     7141 tgcttcctct tttggaagga aagccactag aacaaaagca atgaaaaaag tcagcgagtc
     7201 tagcagtagc gtcatattga gacttgcaaa ctgtaaaaca aggaaggaaa gaacaggaga
     7261 gctaacacct acaacctgca aaaccagctc taagcgagaa ttatagatca caatctcatc
     7321 tttctccacc acttcagtta tgatagcttt attggctgtg cgagaaaagg caaaagcaat
     7381 agcctgcaca atgttagcaa caatcaaagc gccaatcatc cagctatcat tccttatgaa
     7441 agaaatagcc agacaaagaa tcccacaaac aagatctgcc gtcattaaaa tcttacgacg
     7501 agaaaaacgg tctgaaataa ctccgccaaa gggattgacg agaatagatg tgacgagctc
     7561 agaaatctga tacattccta aaactgtctg tcctatagtc cccatagaag ccaaccagac
     7621 actatttcca taatcataga gcatatttcc cattttattg atagccccac ggctaatcaa
     7681 ctgcactgca tagcgattca tattaaagct cctctcaaat tttgaaacta ttgtatcaaa
     7741 accgaaagga gctttttatt ttttccctta tttgggaaaa ttaacttttg acaaattttt
     7801 cgtagtgttc ctgataatag gctacttgct ctggaagacc taacacatca aaaatatgca
     7861 tggccttttg catctgctta cagccttctt tacactgtcc tttttgatat aaggcaaaac
     7921 cttttaaata atggaaaaca ttacgctcat aaagcttaat acctttgtca ataatcttct
     7981 ctgtataagc ctcaaaatag ttggcattat aaaaagaaga atgctctaaa caatgctggt
     8041 aacaattgag ggccaaaatc aaaactaatc tcttatggcg actaatctct tggtaaaatt
     8101 cctccctctc cataacttct ctaccaatcc gagtgacata gtctacatcg tagaaactat
     8161 agaggttacc gaaaagaatc aactcataca tggtccattc ttctgttttg aagagataat
     8221 ctgctacctt atccaaatca tcctgcttca tatcataagt cgcatctctt tgacaaatca
     8281 gaccttgtag caaaatccag ttcagctcaa aataaaggga agtcgtcgaa ctcttagact
     8341 tttcaagttg ttctctttga agcttttgaa aacctgcaat atcgtttgaa tagtaaagtg
     8401 ggataatctg tgccatcata gacacatgtt catgattatg aaaattcctt gccttatcca
     8461 tgaaattttc gattgttaca tgaatgttat ccaaaatctc aaagaaacgg gagactgcca
     8521 ggtcagactc cccaagctca aagcgagata actgagaggt agagcaggat tcgcctgctg
     8581 cttcctttaa agaataattt ccacttgttc gaaattcacg aaatactttt ccaagatgtt
     8641 ccatctttac acctgatctg ataattcttc ccactcaagc atagcttctt cctgacgatg
     8701 gctgattttg tccagctcag cctgtaattc catgagtttg tcggcatcgt ttgtttccaa
     8761 catttgttca gaaatggctt ggctttgact ttctagctct tcaatttcag cttctagact
     8821 ttcgatttgt cgcatgagtt tgcgaacttc tttttgactt tctttctggg cctgatagtc
     8881 attgactgga cttgcttcct ttgcttgatt gctagttgaa gcttcctcag tctgactcat
     8941 ttctgctgtt gctttcttct caacatagta gtcgtaatct ccaaggtaga gagttgaacc
     9001 attctcagac aattccaaaa catgagttgc cacacgattg ataaagtaac gatcatgact
     9061 gacaaaaagc aaggtaccat caaagtcaat caaggcattt tctagcactt ccttactatc
     9121 aatatccaag tggttggtcg gctcatccag aatcaaaaag ttattgtttt ccatagacaa
     9181 tttagctaaa agcaaacgag ctttttcgcc accagatagc atgccgactg attttttaac
     9241 atcatctcct gagaaaagga aggccccaag acggttgcgg atttcaactt ctggtgtcag
     9301 tttgaaatca ttccagagtt catccagcac cgtattactt ggtgtcagct tgctttgggt
     9361 ttggtcatag taaccaacct caacattagc gccaaagcgc ttttcacctt tgataaaagg
     9421 aatctggtcc acaatagact tgataaaggt tgacttgccg ataccatttg gaccaacgat
     9481 agcgacagca ttcatcttac gaagatctag gttgataggt tgtgacaaaa cttccccgtc
     9541 ataaccaata gcagtatttt caacagtcaa aacaacattg cccgacgttt tttcagactg
     9601 gaaggtcatg ttggctgctt tcttgccagc ttcaggcttg tccaaacgct ccattttttc
     9661 tagttgttta cggcgagatt gagcacgttt ggtcgttgaa gcacgaacta gattgcgatt
     9721 gacaaagtct tccagagcag cgatttcctt ctgttgcttt tcatagtttt ttgcctcagt
     9781 agctagcttt tgctccttca actcaacaaa acgagagtaa ttccccacat agcgatccaa
     9841 ggaatacttg gtcaaatcta gcgtaattgt cgcaaccttg tccaagaaat aacggtcgtg
     9901 gctgacgata atgagggcac cgctatagtt taccaagtaa ttctctagcc aggagatggt
     9961 ttcaatatcc aagtggttag ttggctcgtc caagaccaag agattgggct tttcaaggag
    10021 cattttagct agagccaaac gagtattttg accaccagaa agctcagcaa ttttcatctg
    10081 ccacatagac tcgtcaaact tgaatccatt caaaatcgct cgaatatcag cttcataggt
    10141 aaagccacct gcttggcgaa aattttcaga taagcggtca taatctgaca tcagtttatc
    10201 caaatcctca ccagactttt cacccatctc cagctccatc tgacgcagtt gtttatctgt
    10261 ccgacgtaag tcatcaaaga catgaagcat ctcatcatag atggtatttt cagactcaaa
    10321 acggctatct tgagctaggt aagacagaga aatatctttt ttcttattga tttctccact
    10381 agttggctcc tcttctccaa ctaaaatctt caaaagagta gacttacctg caccattttt
    10441 cccaacaagg gcaatccgat ctcgttcatc aacctgcagg ttgatattat cgaaaagaac
    10501 ctctcctgca aaagaacgtt caattttatt agcttgtaaa ataatcatac aagtagtata
    10561 gcatgtttcc ctaaggcatt caagataatc gtaagtcttt tagtacaact tttataacat
    10621 aaaataaact aaattatgta tattttatat tagattcctt cactatcttg ttggattttc
    10681 taaccagcta atcttgtttc aaatagttat cgcacaagcc tattatttaa ttcttttcat
    10741 tat
//
LOCUS       DAYYQN010000061        10737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYYQN010000061 DAYYQN010000000
VERSION     DAYYQN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.062"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            150..647
                     /locus_tag="ACHBRQ_001689"
     CDS             150..647
                     /locus_tag="ACHBRQ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9593314.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            647..2302
                     /gene="dnaX"
                     /locus_tag="ACHBRQ_001690"
     CDS             647..2302
                     /gene="dnaX"
                     /locus_tag="ACHBRQ_001690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9593315.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2330..2524
                     /locus_tag="ACHBRQ_001691"
     CDS             2330..2524
                     /locus_tag="ACHBRQ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HFM9593316.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2674..3444
                     /gene="sufC"
                     /locus_tag="ACHBRQ_001692"
     CDS             2674..3444
                     /gene="sufC"
                     /locus_tag="ACHBRQ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9593317.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3472..4734
                     /gene="sufD"
                     /locus_tag="ACHBRQ_001693"
     CDS             3472..4734
                     /gene="sufD"
                     /locus_tag="ACHBRQ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9593318.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGDDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4745..5971
                     /locus_tag="ACHBRQ_001694"
     CDS             4745..5971
                     /locus_tag="ACHBRQ_001694"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9593319.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5958..6398
                     /gene="sufU"
                     /locus_tag="ACHBRQ_001695"
     CDS             5958..6398
                     /gene="sufU"
                     /locus_tag="ACHBRQ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9593320.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6452..7864
                     /gene="sufB"
                     /locus_tag="ACHBRQ_001696"
     CDS             6452..7864
                     /gene="sufB"
                     /locus_tag="ACHBRQ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9593321.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8095..9336)
                     /gene="pbp3"
                     /locus_tag="ACHBRQ_001697"
     CDS             complement(8095..9336)
                     /gene="pbp3"
                     /locus_tag="ACHBRQ_001697"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HFM9593322.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKNLIGQGYITTERPSFEMIADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9439..10548
                     /gene="cozE"
                     /locus_tag="ACHBRQ_001698"
     CDS             9439..10548
                     /gene="cozE"
                     /locus_tag="ACHBRQ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HFM9593323.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3180 a         1960 c         2338 g         3259 t
ORIGIN      
        1 catatgggac tttttttcta caaaaaaata ggctccataa tatccatagg ggatttaccc
       61 actacaaata ttatagagcc caaaatcctt tgtttactaa acaagggatt tttcttttgt
      121 ctctgctcct tttttgatat aatagttcta tgttaaaatc agaaaaacaa tcacgttatc
      181 aaatgttaaa tgaagaattg tccttcctat tggaaggcga aaccaatgtt ttggctaatc
      241 tttccaacgc cagtgctctc ataaaatcac gttttcctaa taccgtattt gcaggctttt
      301 atttgttcga tggaaaggaa ttggttttag gtcccttcca aggaggtgtt tcctgcatcc
      361 gtattgcact aggcaagggt gtttgtggtg aggcagctca ctttcaggaa actgttattg
      421 ttggagatgt gacgacctat ctcaactata tttcttgtga tagtctagct aaaagtgaaa
      481 ttgtggtgcc gatgatgaag aatggtcagt tacttggagt tctggatctg gattcttcag
      541 agattgagga ttacgatgct atggatcgag attatttgga acaatttgtc gctattttgc
      601 ttgaaaagac agcatgggac tttacgatgt ttgaggaaaa atcttaatgt atcaagcact
      661 ttatcgaaaa tatagaagtc aaaacttctc ccagttagtt ggtcaagaag ttgtggctaa
      721 gactcttaaa caagcggtgg agcaagagaa aataagtcac gcttatcttt tttctggtcc
      781 tcgtggaacg ggaaaaacca gtgttgctaa aatctttgcc aaggctatga actgtcccaa
      841 tcaagtgggt ggcgaacctt gcaataactg ctatatttgt caagcagtga cggacggtag
      901 tttagaagat gtcattgaaa tggatgcagc ttctaataat ggggtagatg aaattcgcga
      961 aattcgtgat aaatctacct atgcgcctag ccttgctcgt tataaggttt atatcataga
     1021 tgaggttcac atgctgtcta caggggcttt taatgccctc ctaaagacgc tggaagaacc
     1081 aacacagaat gtagtcttta ttttggccac tactgaattg cacaagattc ctgctactat
     1141 tctatcccgt gtgcaacgtt ttgagtttaa atcaattaag acacaggata ttaaggaaca
     1201 tattcactat atcttagaaa aagaaaatat cagttctgaa ccagaggctg tggaaatcat
     1261 tgccagacgg gcggaaggtg gaatgcggga cgccttgtct attttggatc aagccctgag
     1321 tttgacacag ggaaatgagc tgacgactgc tatctctgaa gaaattactg gcaccattag
     1381 cctatcagcc ttggatgatt atgtggcggc cttgtctcaa caggatgttc ccaaagcttt
     1441 gtcttgcttg aatcttcttt ttgacaatgg taagagcatg actcgttttg tgaccgatct
     1501 tttgcactat ttaagagact tgttaattgt tcaaacaggg ggagaaaata ctcatcatag
     1561 ttcagtcttt gtagaaaatt tggcacttcc tcaaaaaaat ctgtttgaaa tgattcgctt
     1621 agcaacagtg aatttagcag atattaagtc tagtttgcag cccaagattt atgctgaaat
     1681 gatgaccgtc cgtttggcgg aaatcaagcc cgaaccagct ctatcaggag cggttgaaaa
     1741 tgaaattgct acgctgagac aggaagttgc ccgtctcaaa caagagcttt ctaatgcagg
     1801 tgcggttcct aaacaagttg caccagctcc tagtcgacca gctacgggca aaacagtcta
     1861 tcgtgtcgat cgcaataaag tgcaatctat cttacaagag gccgtcgaaa atcctgattt
     1921 agcacgtcaa aatctaattc gtttgcagaa tgcctgggga gaggtaattg aaagtctagg
     1981 tgggccggac aaggctctgc tagttggttc tcaaccggtt gctgccaatg aacaccatgc
     2041 tattcttgct tttgagtcta acttcaatgc tggtcaaact atgaaacgag acaatctcaa
     2101 taccatgttt ggtaatatcc tcagtcaggc ggcaggtttt tcacctgaga ttttagctat
     2161 ttccatggag gaatggaaag aagttcgcgc agccttttca gccaaagcca aatcttctca
     2221 aactgaaaaa gaagtagaag aaagcctgat tccagaagga tttgaatttt tggctgataa
     2281 agtgaaggta gaggaagact aaagaaagat ttcatgatac aataagttta tgaataaaca
     2341 acaatttatt attatggcgc tatttacagc tgctgagacc tattttttca atgaagcctg
     2401 gatgactggc cgctatatta tggcagcctt ttgggcaatt ttactcttta gaaatttccg
     2461 agtcagttat gtgatgggca aaatcgttga tgtcatcgat cagcatttta ataggaaaga
     2521 ctagccctca gcttccagac aaaatcaaag ccttttaggc ttttttttgt tatactagaa
     2581 aagtatattt atagaatttt tgctctattt ttggggaaat cagacgtttt tctagtaagt
     2641 actgtaaaag ttttgaaaaa gaaaggaact atcatgtcag tattagagat caaagatctt
     2701 catgttgaga ttgaaggaaa agaaatttta aaaggggtta acctgaccct gaaaacagga
     2761 gaaattgccg ctatcatggg accaaatggt acaggtaaat cgactctttc tgccgctatc
     2821 atgggaaatc caaactatga agtaactaaa ggtgaagttt tgtttgatgg cgtaaacatc
     2881 cttgagttgg aagtggatga gcgtgcgcgt atgggacttt tccttgctat gcaataccca
     2941 tcagaaatcc ctggaattac caatgctgag tttcttcgtg ccgctatgaa tgcgggtaaa
     3001 gaagatgatg agaagatttc agttcgtgag tttattacta agctagatga aaaaatggaa
     3061 ttgctcaaca tgaaagaaga aatggcagag cgttacctca acgaaggctt ctctggtggt
     3121 gagaaaaaac gcaatgaaat tcttcaactt ttgatgttgg agccaacatt tgctcttttg
     3181 gatgagattg actcaggtct tgatattgac gctcttaaag ttgtgtctaa aggtgtcaat
     3241 gccatgcgtg gtgaaggttt tggtgctatg atcattactc actaccaacg tcttttgaac
     3301 tatatcacac ctgatgtggt acacgtgatg atggaaggtc gtgttgtcct ttctggtggt
     3361 ccagaattgg ctgcgcgttt ggaacgtgaa ggatacgcaa aactagctga agaacttggc
     3421 tacgactaca aggaagaatt gtaattccct cgtatctttt aggagaagta aatgactaga
     3481 gaaaatatta aacttttttc agaaatgcac gctgaaccaa gctggttggc tgatctccgt
     3541 caaaaagctt ttgacaagat tgagactttg gaattaccag ttattgagcg tgtcaaattc
     3601 caccgttgga atctgggtga tggaacgatt acagaaaatg agccatcagc aaatgttcca
     3661 gatttcacag ctttagatca tcacttgaag ttggtgcaag taggaactca aactgttttc
     3721 gaacaaactc cagttgagtt agctgaacag ggtgttgtct tcacagactt tcactcagct
     3781 ttagaagaaa ttccagagct gatcgaagaa ttcttcatgt catctgttaa gtatgatgat
     3841 gacaagttgg cggcttacca cacagcttac tttaacagtg gtgctgtact ctatattcca
     3901 gataacgtag aaattacaga gccaattgaa ggaattttct accaagatag cgatagcaat
     3961 gtgccgttta acaagcatat tatgattatc gctggtaaaa attctaagat tagttatctg
     4021 gagcgtttag agtcacgcgg tgaaggaagt gacaaagcaa ctgccaatat cacagtggaa
     4081 gtgattgcac gttctggtgc gcaagtcaag tttgctgcta tcgaccgtct aggtgaaaac
     4141 gtcactgcct acattagccg tcgtggtaaa ttaggcgacg atgcaagtat tgactgggct
     4201 atcggtgtca tgaacgaagg aaatgtcgtt gctgattttg atagtgactt gattggtaat
     4261 ggtagccatg ctgacctgaa ggttgtagct ctttcaagtg gtcgtcaggt acaagggatt
     4321 gatactcgtg taactaacta tggctgcaac tctatcggaa atatcctaca acatggggtt
     4381 atccttgaaa aagcaacttt gactttcaat ggtatcggcc acatcatcaa gggtgctaag
     4441 ggagcagatg cgcaacaaga gagccgtgtt cttatgcttt cagaccaagc gcgttcagat
     4501 gctaacccaa ttcttttgat tgatgaaaat gacgtaactg caggacatgc agcctctatt
     4561 ggtcaggtag atccagaaga catgtattac ctcatgagtc gtggcttgga taaggcaact
     4621 gcagagcgtt tggttgttcg tggtttcctt ggatctgtta tcgtggagat tccagtcaag
     4681 gaagttcgtg atgaaatgat tgcaactatc gaagagaaat tgtcaaaacg ctaaggggaa
     4741 gcctatgtta gatgtagaag cgattcgcaa ggattttcca attttagatc agattgtcaa
     4801 tgatgaacct ctggtctatc tggacaatgc tgcgacgaca caaaaaccac tagtagttct
     4861 gaaagctatt aacagctact atgagcagga caatgccaat gttcaccgtg gtgtccatac
     4921 cttagcggaa cgagcgacag cttcttatga agctgctcgt gaaaccattc gtaagtttat
     4981 taatgcaggc tctacaaagg aagttctctt taccagagga acgacaacca gccttaactg
     5041 ggtggcacgc tttgctgagg aaattctcgc tgagggagac caggtcttga tttcagtaat
     5101 ggaacaccat tctaatatca ttccatggca ggaagcttgt cgaaagactg gagcagagct
     5161 tgtctatgtc tatcttaaag acggtgcctt ggatatggag gatttgcgag ctaaattgac
     5221 tgataaggtt aaatttgttt ccctagctca tgcctccaat gttcttggtg tggtcaatcc
     5281 gatcaaggaa atcactcaat tagcccacca agttggggca attatggtag tggatggtgc
     5341 tcaatctaca cctcatatga agattgatgt ccaggacttg gatctggact ttttcgcctt
     5401 ttcgggtcac aagatggctg gtccgactgg tatcggtgtc ctttacggca aagaaaagta
     5461 tcttgagcaa atgtctccag tagaatttgg cggcgagatg attgattttg tctacgagca
     5521 atttgctagt tggaaggaat tgccttggaa atttgaggct ggaacgccaa atatggcagg
     5581 agctattgga cttgcgactg cagttgatta tctggaaaag attggtatgg atgccgttga
     5641 agctcatgaa caggaattga ttgcgtacgt ctatccaaaa ctgcaggcaa ttgagggatt
     5701 gaccatttac ggttctcagg atttggctca acgttcgggt gttattgcct ttaacctagg
     5761 tgatctccat cctcacgatc ttgcgacggc tctggattat gaaggagtgg ctgttcgtgc
     5821 tggtcaccat tgtgcgcaac ccttgcttca gtatttggaa gtcccagcaa cagctcgtgc
     5881 aagtttttat atctacaata ccaaggcaga ttgcgacaaa ctagtcgatg ccctacaaaa
     5941 gacaaaggag tttttcaatg gcactttcta aactagatag cctttatatg gcagtggtag
     6001 cagaccattc gaaaaatcca catcaccaag ggaagttaga agatgctgag caaaccagtc
     6061 tcaacaatcc gacttgtggg gatgtcatca acctctctgt caagtttgat gcagaggacc
     6121 gtttggaaga tattgctttt ctaaattcag gatgcacgat ttcaactgct tctgctagta
     6181 tgatgacaga tgccgtttta ggaaaaacca aacaagaaat tttagaactg gcgactattt
     6241 tttctgaaat ggttcaaggg caaaaagatg agcgtcaagg ccaacttgga gacgcggcat
     6301 tcttgtcagg tgttgccaaa ttccctcaaa gaatcaagtg tgcaacccta gcttggaatg
     6361 cccttaagaa aacaattgaa aatcaagaaa aacagtaaga caagtttctt ttgtcttatg
     6421 aattattaga aatgaagaaa gaaaggatac tatggctgaa gaaagagtag aaccaaaacc
     6481 aattgacctt ggtgaatata aatttggttt ccatgacgat gtagagcctg tcttatcgac
     6541 aggaaaagga ctcaacgaag gtgttattcg tgaattatct gctgctaagg gtgagcctga
     6601 gtggatgttg gagttccgtt tgaagtctta tgaaaccttc aaaaaaatgc ccatgcaaac
     6661 ttggggagca gacttgtcag agattgactt tgatgactta atctactacc aaaaaccatc
     6721 tgacaaacca gcccgttctt gggatgatgt acctgaaaag attaaagaaa cctttgaacg
     6781 tatcgggatt ccagaagctg aacgtgctta tttagcaggg gcttctgccc agtacgagtc
     6841 agaagtggtt taccacaaca tgaaggaaga gttccaaaaa ttaggtatta tctttacaga
     6901 tacagattcc gcactcaagg aatatccaga cttatttaaa caatactttg cgaagttggt
     6961 accgccgaca gataacaagt tggcagccct caactcagca gtatggtcgg gtggaacttt
     7021 tatctacgtg ccaaaaggtg tcaaggtaga tattccactt caaacttatt tccgtatcaa
     7081 taacgaaaat ataggtcagt tcgaacgtac cttgattatc gttgatgagg gagcaagcgt
     7141 ccactacgta gaaggatgta cagcaccaac atattcaagc aatagcttac acgctgccat
     7201 tgtagaaatt tttgctttgg acggagctta tatgcgttat acaactatcc aaaactggtc
     7261 tgataacgtc tataacttgg taacaaagcg tgctaaggct caaaaggatg ccactgttga
     7321 gtggattgat ggaaacttgg gtgccaaaac gactatgaaa tatccatctg tttaccttga
     7381 tggagaagga gcgcgtggta ccatgctctc tatcgccttt gctaatgcag ggcaacacca
     7441 agacacgggt gctaagatga ttcacaatgc tccacatacc agctcgtcta ttgtgtctaa
     7501 atccatcgct aaaggtggag gaaaggttga ctaccgtgga caagtcacct ttaacaagaa
     7561 ctctaagaaa tctgtttccc acattgaatg tgataccatt atcatggatg acttgtcagc
     7621 atcagatact attccattta atgaaattca caactcgcaa gtggctttgg aacacgaagc
     7681 caaagtatct aagatttcag aagagcaatt gtattatctc atgagccgtg gattgtcaga
     7741 atctgaggca actgaaatga ttgtcatggg atttgtagaa ccctttacaa aagaacttcc
     7801 aatggaatac gcagttgagc tgaaccgctt gattagctat gaaatggagg gatcagttgg
     7861 ataaaatttg attttatact cttcgaaaat ctcttcaaac cacgtcagca tcgccttacc
     7921 gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaacagt gttttgagca
     7981 acctgcggct agcttcctag tttgttcttt gattttgagt attagattta ctcaaaatca
     8041 aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga ttttttataa
     8101 tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga aggctttttc
     8161 aattttcttg tctgctatca tttcgaaact agggcgctct gtggtgatgt aaccttgacc
     8221 aatcaagttc ttgtcttcat aagtcaaatg gccaaccact gttccagctt caagtggtgc
     8281 tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat tcccaacacg
     8341 ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt ttccatcttg
     8401 tacaggggct ttgctatctt gataggcatc gccttgttga acgattttgc gaagtgtaaa
     8461 tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt aaggattatt
     8521 gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt cgacagtagt
     8581 accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat caaaaccacc
     8641 acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca tcccagcaaa
     8701 agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga tgaggttgcg
     8761 agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt tagaacctgg
     8821 gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa cagtggcatc
     8881 ctgaattccc cattccaaga gttttgcccg catcatatcg acgaaatctt tttctgagcc
     8941 agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca gagttgcttc
     9001 aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg cttcagaatt
     9061 tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg taatacttcc
     9121 gttttccaaa gcttcataga ccagataaac agtaatcaat tttgttatgg aagcaatttc
     9181 gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct caacagcaat
     9241 cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac cccctaaaag
     9301 agagacagtt aacaaagtta aaaatatttt tttcatagta gttttattct atcataaaga
     9361 aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata tggtaaaata
     9421 aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac cacagaaatt
     9481 ttactcttaa ccatcatctt ttacctatgg agacagatgg ggtctttgat taaccctttt
     9541 gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct ttattatttg
     9601 acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt gcttggtatt
     9661 ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta tctcttacct
     9721 attttgatta atcagttatc tagtttgatt atatctagtc aaactattta tagtcgagta
     9781 caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga tgtagaagct
     9841 acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct aaatagcgta
     9901 tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat tttgattatg
     9961 actccagttt ttttggttta tttcttatta gatggacata aattcttgcc catgcttgaa
    10021 agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa tttaaatgcg
    10081 acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg ttgtttagct
    10141 tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat tttttctggt
    10201 gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat gatcatcgca
    10261 aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct tgttgttcag
    10321 caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa ggttcatcca
    10381 atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt tggaatgatt
    10441 gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc ccgtttgtat
    10501 gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa gtcaggagaa
    10561 ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat aagttaaagt
    10621 cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa tcgaaaaaac
    10681 cagtggaatt ccgtgtcagg gtaagttcca ctggttttca tagtctatta aagttcg
//
LOCUS       DAYYQN010000062        10673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYYQN010000062 DAYYQN010000000
VERSION     DAYYQN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.063"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..656
                     /locus_tag="ACHBRQ_001699"
                     /pseudo
     CDS             1..656
                     /locus_tag="ACHBRQ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(784..1806)
                     /gene="cbpF"
                     /locus_tag="ACHBRQ_001700"
                     /pseudo
     CDS             complement(784..1806)
                     /gene="cbpF"
                     /locus_tag="ACHBRQ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1825..2646)
                     /gene="cbpG"
                     /locus_tag="ACHBRQ_001701"
     CDS             complement(1825..2646)
                     /gene="cbpG"
                     /locus_tag="ACHBRQ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HFM9593324.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVNTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGNWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2727..2966)
                     /locus_tag="ACHBRQ_001702"
     CDS             complement(2727..2966)
                     /locus_tag="ACHBRQ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593325.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3116..3822)
                     /locus_tag="ACHBRQ_001703"
                     /pseudo
     CDS             complement(3116..3822)
                     /locus_tag="ACHBRQ_001703"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3885..4517)
                     /locus_tag="ACHBRQ_001704"
     CDS             complement(3885..4517)
                     /locus_tag="ACHBRQ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9593326.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4531..5526)
                     /locus_tag="ACHBRQ_001705"
     CDS             complement(4531..5526)
                     /locus_tag="ACHBRQ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9593327.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5523..6221)
                     /gene="liaF"
                     /locus_tag="ACHBRQ_001706"
     CDS             complement(5523..6221)
                     /gene="liaF"
                     /locus_tag="ACHBRQ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9593328.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6298..7308)
                     /gene="fni"
                     /locus_tag="ACHBRQ_001707"
     CDS             complement(6298..7308)
                     /gene="fni"
                     /locus_tag="ACHBRQ_001707"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9593329.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7292..8299)
                     /locus_tag="ACHBRQ_001708"
     CDS             complement(7292..8299)
                     /locus_tag="ACHBRQ_001708"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9593330.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRADIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFFAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8286..9239)
                     /gene="mvaD"
                     /locus_tag="ACHBRQ_001709"
     CDS             complement(8286..9239)
                     /gene="mvaD"
                     /locus_tag="ACHBRQ_001709"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9593331.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(9221..10099)
                     /gene="mvk"
                     /locus_tag="ACHBRQ_001710"
     CDS             complement(9221..10099)
                     /gene="mvk"
                     /locus_tag="ACHBRQ_001710"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9593332.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQAVLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10221..10550)
                     /locus_tag="ACHBRQ_001711"
     CDS             complement(10221..10550)
                     /locus_tag="ACHBRQ_001711"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9593333.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNDTLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         3149 a         2486 c         1883 g         3155 t
ORIGIN      
        1 gacctactac tatcacttga aacagctaga caaaccagat aaggaccaag agcttaaagc
       61 tgaaattcaa tccattttta tcaaacacaa gggaaattat gcttatcgcc gggttcattt
      121 agaactaaga aatcgtggtt atctggtaaa tcataaaaga gttcaaggct tgatgaaagt
      181 actcaattta caagctaaaa tgcgacagaa acgaaaatat tcttctcata aaggagacgt
      241 tggcaagaag gcagagaatc tcattcaagg ccaatttgaa ggctctaaaa caatggaaca
      301 gtgctacaca gatgtgactg aattttccat tccagcaagt actcaaaagc tttacttatc
      361 accagtttta gatggcttta acagcgaaat tatcgcctat aatctttcaa cttcacccaa
      421 cttagaacaa gtacaaacaa tgttggaaca ggcattcaca gagaagcact atggtggagt
      481 ccttctttgg gattctgaaa tcggaaatgt tttatggcta tgagaaaaca tttaaatcac
      541 ttaatcaatt ggaacaagcc attatagact atattgatta ttacaacaac aagagaatta
      601 aggtaaaact aaaaggactt agtcctgtgc agtacagaac taaatccttt ggataaatta
      661 tttgtctaac ttttgggggg cagtacagaa aaaccttgat actatgcgtt ttttgattgt
      721 gaagactttt atccacctat tatacaaagt taagtatcca tcacagttta caattagcct
      781 tcattactta acccattcac cattatagtt taagtagtaa ccacctactg tggtattaac
      841 agctaaagca cctgaatcat aggcatagta ccagttacca ttgacttgga accagcctgt
      901 tttcatatct ccattacttg catttagata gtatcaagtc gaaccttcct gataccaacc
      961 agttgccata gctcctgatg aacggagata gtaccatttg ttcccaaggt tttgccagcc
     1021 agttctcata tcaccatttt cagcatctag atagtaccaa gttgagcctt ccttatacca
     1081 gccagttgcc atagctcctg acgaatggag gtagtaccac ttattaccta gatattgcca
     1141 acctgtttgc ataatgccag ttgctggatc tagatagtac catggagctg cttttagctt
     1201 ctcttcatca tacgtaagag ggtaatcctt aacccaacca cgtgctagct ctccaaccgt
     1261 caatctgttg atgcgagaat caaagccacc atccttctgt aaataatacc aatgaccctc
     1321 ttcataaatc caaccagttt ttaaagtatg ataactacgc tgatcttcaa aataatagac
     1381 tcgtttctct gcttggctat catattcttc cccatgatgt ttgttggtat tcgtagcagt
     1441 ttttgcttct aaaacttgct tgccaacaaa ttcttgtaag acaccatctt gaccaaaata
     1501 aaaccaatct ggtctaagag caatctctat tcttggagaa ggaccaatcg taaccccctt
     1561 gtgtggagca ggtatatatt gccagccgac aaccatctct ccggataaag gatcaaaata
     1621 atagtacttc ccatctatca ctctccagta ggtttctttt agagccccct ttttgtagta
     1681 ggttctacca ttttctttga caaactgcca tccttcagaa tcatctgcaa atactgtact
     1741 ggtccctagc aaaccgaaga aaaatgtggc tagtgcgact tgcatcattt ttttcaaaag
     1801 cttcatttta tatatcctcc aacatcaaat ccactcacca gatgaagcga agttataaac
     1861 tttaccatcg atagtttggc tacctgtaac cattgctcca tttgaattga gataatacca
     1921 gtttccattt actttttgcc aatctgtaag catcttaccg gaactgtcta aataatacca
     1981 agtatcattt atctcctgcc aacccgtttg tttatcatgt tgtctataat agtaccaact
     2041 accatttatt ttcttccatc cttcaagaga gtaaccttta agaaccgaat aaataaatgg
     2101 caaatttcgt tcatttaatt taactgcact gttaatttga ttagctccat ctcctaaagt
     2161 atgcactcct actacacggt gactagcatc ataaactgta gatccactag acccctctaa
     2221 agtattaact tggtaatcca agaacatgcc atcatcactt aaaacttgtt ttttatctgt
     2281 atacatttga tgaattttaa aattatatga tagatagcct gtgatagtca cagttattcc
     2341 tgtcaaattt ttttgactag taggaagacc caaagtccct aattttgcac caatgggctc
     2401 ttctagaatt aataaagcca agtcatattc ccttgcatcc ttagaattta aatttctaaa
     2461 ttccttcaaa taacgaactt cctttacttt gatctttcca aatagttctt gacttggact
     2521 aacagccgga agaacataaa tatcatccgc ttctttccca tagtcatgtc tgtagtagtt
     2581 gtgagccgct gtaattaaca cattatcttt aataaaattc gcacttcccc tcccttctac
     2641 attcattcca tcggctactc cataatactt agataaaacc atagctgaag tcgaatacgg
     2701 atactgtaaa gtattatcaa ttttaatcaa atcatcatta ccgataatac ttctgattgc
     2761 ttttggtagt atgaaccata cgttggtgaa atctcagata atgaagaatc attagactct
     2821 ggaccttttt ctagtgtctc acttacctca tattcttcac ctgtactaga aataacactc
     2881 aaagcagata ctgtcgataa ctggctagcc aataaagtac tcgcaataat tgaaataccc
     2941 aattttttat aaacagtttt cttcattatt gtatcctcct aatgtaatta tagcgtacta
     3001 ttctaaattt cttaatctac tatagaatca agaaatctac caccttcttt aaataccctc
     3061 cattatcaca taaacaggta aacttttcaa ttaatgactg cgcttttcaa tcacgctaga
     3121 ggtacttgct tgcttctttg atactaagtt cagccattct ttccttgttt ttctcaataa
     3181 agcatgttac ccaagtggga ttcgttttgg agtagtctcg cagagtccag ccaatggctt
     3241 tattgataaa aaattctgtt tggttcaagt tatgaagtag aatcttttcc attaattgag
     3301 tattggtctt ctcttttctt aacaactggt ggtcaatagc gacacgtctc agccagatat
     3361 tatctgatag gctccatttt atactcaatg aaaatcaaag agcaaactag gaagctagcc
     3421 gcagattgct caaaacactg ttttgaggtt gcagatagag ctgacgtggt ttgaagagat
     3481 tttcgaagag tattaagatt atttcttcta gttcagagtg ttcatacacc aaactcccta
     3541 ctactcgatc taggatatct accgtgtccc accaggattt tgtcacgact aactgctcta
     3601 gcttaggcaa atcggtttcc tttagataag actgcattgc tttcaaatag ttagcagcca
     3661 catattggta ttctctagga tccttttccc agcaagtgtc tgcaaaatcc caatcgataa
     3721 tctttgtttt tttcgcttct ggaaaatatt tttatagagt ttatttcttt caggcaccgc
     3781 aatacctaga aaagaaaatt gatggcgcat ataggcttcc atgggccttg ctttttagag
     3841 tcttttgctg cttctagctc ctcaagtaaa tctgctaaac tcatctaaaa ctcctcatgc
     3901 cccaccaaat ggtgctgaaa ggcatagaca gccgcctggg tacgatcgct gacttcaagt
     3961 ttggcaagaa tgttggacac gtgggtcttg accgtcttga gagagataaa gaggtcatct
     4021 gcgatgcgct gattttcgta gcccttggcg atgagttgga gaacatctcg ctcacgcgca
     4081 gtcaattctt catgaagttc catatgattg cggtggtatt caaccttctt gctaacctct
     4141 tgctcaatgg ccagctcgcc agcagctacc ttactgacgg catgaagcaa ttcatctgca
     4201 ctagaagtct tgagcatata gcctttggca ccagcatcta agactggcat gattttttca
     4261 ttgtccaaat aagaggtcac aatcaaaatc ttggcttcag gccattcttt aaggattgct
     4321 aaggtcgcgt caatcccatt catctcaggc atgacaatat ccatgacaat gacatctgga
     4381 cgcagttcca aggccaagtc aatcccttga gacccgttgg acgcctcacc tacaacttct
     4441 acatcgtctt ggaggtcaaa gtagcttttc aagcccaatc ggaccatttc atggtcatct
     4501 actagtaaaa ttttcatctt tactccttta tcattcctta tctaacaggg gaatacggat
     4561 atcaaccgcc agcccttgct tgggagctgt caagagttga actgttccag ccatatcttc
     4621 aacccgctcc ttgatatttc gcagtccata actcaagtcg tctaagctcc ctaactggaa
     4681 accaatccca ttgtccacca ccttcagttg caattcaaca tctgtctgat agaggtagac
     4741 atctaggcaa gatgcctggg catggcggag cgtattgcta atcaactctt gcaggatacg
     4801 gaagatatgc tcctcgattt tcttaggcaa tttcgtcata ttctgcttga gactaaccct
     4861 aagatcactc ttgtcctcaa gctcttttaa aagaatttga atcccttcta tcaagctctt
     4921 ctgctccagt tcaactggtc gcaaatgcaa gagcaaaacc cgcaaatcct tctgggctgt
     4981 ttctaaaata gctgtgacac tctgcaactg ggtctgcatc ttttctctat ccaatttcaa
     5041 agcctgctga ctgatacccg ataaaatcat gtgggccgca aacaactcct gactgactgt
     5101 atcgtgcaaa tcccgagcaa ttcgcttccg ttccttctcg atgatttcct cttcctgagc
     5161 aaggctatga ttttcagctt tttgaagagc ctctgtcaaa aggttaagtt tacctgataa
     5221 ggacttgaaa ctggcatcca aatctggatc tacaacctga accacttctt gccctgccaa
     5281 taaacgcttg agattagcct gcatttttct tagagaaagc tcttcgatcc ctcgccaaaa
     5341 cagggctaag agacaggtca tggacatgct gaaaaccaac aataaaaaga aaaatttttc
     5401 tgttttttcg acatcgtgca aaaagataga ccagtcaaaa tcaagtattt ccagcaagct
     5461 atgggagaaa aaaaagacaa acaggaagga ggtgagagcg ataatgacat aggcttgttt
     5521 tttcatcctc taaccacctc aacatcacca atcatagtgg tcaagaaaat cttgacactc
     5581 ttgttactct tgagatagtc ttttgtttct tgatgatagt gttcattgcg gagggctcgc
     5641 ttgggctggt tgaaaaaagt caaatcccca tagagacagt taacgctgag actgacttcc
     5701 acatctacag gtacgatgat tttggtcgtt cctaccatct ttctgaggat aatgacattg
     5761 tcatgattgg ttaagatgac cctctccaga tgaatagtgt ccttgcccat gaagcgaaag
     5821 agattgatat catcaaattg gcaagtctgg tagcttgaaa aatgatgaag atttccaaac
     5881 caacgatttt tctccttctt aaccgtcaca acctcttcaa aaaccaaatt ggtctgctct
     5941 ttttcctggt tcatcatcgg gtaaagaaga aagagactat agataaccgc aacaaaaata
     6001 gctagaatca caaaaggatt gagcataacg atgaaaaaga agagaatggt tgccgctact
     6061 aaaagaagat tattcccctc tttaccagtg tagtagcgaa tcaaaagcaa aaagaggaat
     6121 agtatcagca gaaaacgcga aaaatgctct gataccatca aaatcagagc tcctgtcaga
     6181 agacaggctt cgataaataa aaagatttta aattttctca taggttcatc ctctcccttc
     6241 tattttatca caattcaaaa aagtcacctc agtctgagga tggaaaaaag gcgctggtta
     6301 cgcctttttc atctgatcct ttgcttcttt taattttcca taaagaagat agtctacttt
     6361 ttgtagatct gctatggtgg cacagttaag ggaacacata atcaagcgta ggtctgcttt
     6421 ccagccttgg acaatgccaa tcacttcttc aactgtgtag gtttcaacca attccagaac
     6481 ggttcgtgac aatcccacag acttagcacc aaaaaccaag cacttaatca tatccagcgg
     6541 attccgaacc cctccactaa ccaagagttc gaccttatct ttccattctt gggcattgag
     6601 aagggcctgc atggtagact gaccccattg attgaggtaa tcacgctggc cactacgacg
     6661 gttttcgata taggcaaagc tggtgccacc acgacccgat aggtccactg tacgaacacc
     6721 gaattcatag gctctttcga ttgtcttggc atccattcca aagcccactt ccttgaggac
     6781 aataggaacg ggaatttgct cgctataatc tgctagatgc gattgccagc ttctaaactt
     6841 cctttctccc tcgggcatga gtaattcctg catgacattg acatgcactt gcaatagaac
     6901 aggattcatc tcttctacag tctgaagtcc taactcgaca ggcttgtcca atccaatatt
     6961 ggttccaagg aggagattgg gatgactaga cttgacagaa aaagaatcat ccgttggatt
     7021 tttgagggct gcgctataag aacccgttac aaataaaata ccacaggatt ccgccacctg
     7081 agccagcttt tgattgattt ctcttccctt attacttcca ccagtcatgg cattgatata
     7141 aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa agatcgattt catccagatt
     7201 gtaaagaggc aaggaagaat gaatcagctc cacctcatca aagctattat aggaactttt
     7261 ctgctcaagg gcatagagga tatgctcgtc cttacgattt gtcgtcatgt cctatccttt
     7321 cttgatataa gagctcaatc cccagatcgg cccaacgatt ttttaaggtt tcggttgatt
     7381 gcgcatcaaa actcaaggcg atgccacagt caccaccacc agcaccacta ctcttggcaa
     7441 cggcctgcaa atcttgactg gcttctttca actgtctaag caaaggcgtg taaatatctg
     7501 tactcaagcc ttctaaaagc ttgctggcta cttctacttg ctcgataact ttttcggctt
     7561 tcccctgctc caaggcttcg accaaagaaa ccaccgtttc ttttgaggaa cttaaaaaat
     7621 tttgattgat attttgcttg atttgctgga ccatgtgact cgatacagcc acttccttgg
     7681 tccatcccac taagaaatca cattctaaag ttggtttcac ttgtgagata aaaaatcccc
     7741 aatcacgctc cagaactgtc gccaagtttt cttcttctaa ccaagcagcc accttctggc
     7801 gatcaaatga ctggtagaga accaaatcct ctgccacaat acaggcaagg tcgcccatgg
     7861 aaccattgtc tcctcgcttg agcaagacag cgctagtcag cttgaacaag agctcctgat
     7921 caacagaaac atcatacaga gccagtaaag ccttgacaac caagacaacg acgctgccac
     7981 tagaacctag accaaacttt ttcccttctc gttccatttt gccacagatt tctagggaaa
     8041 aaggtcttaa attctgaccg cgaacagcga agaagtctcc catcaaagca atcgtttctt
     8101 gaatcaagct gtagtcagga ttgggcctta agtccactgc gaaatcaaac atatctgaat
     8161 agatacggta gctgtcagaa aaagcaatat cagccctcat atagatggga atatccttta
     8221 tcaaagctaa ctgccctggc tctaaaatag catattcacc tgcccaatag agttttccgc
     8281 aagttttaac agcaatcatc ttgactcaaa tcctttgttt ttgacacaat caagcgataa
     8341 cgctgaccga aaatttctga taaatgctcc aagtctttct cctgacagaa gaccttaaca
     8401 ttgggaccag catccatggt aaagtagcag gcctctcctt tctcacgaag ctggcgaaca
     8461 aagtccatag cctcataaga ggcatccgtc agataagaaa aggctggact agcagtcttt
     8521 gtcgtagcat gcatagccag ggcatttttc tccgttaatt ctccaatctt ggcaaaatca
     8581 ttttccttga gataaatcag catatcctga tagtccttct cagactgacg aacccagtcg
     8641 tcaaaagtcg tcgaggtttc cacacaaagt ttcatcccat cacggctaga gattggtttt
     8701 ttcttgtcct ctagcaccaa cataatcata gctagtttca agtctgtctc tacagggtaa
     8761 atttctccac tatccttatc ccaggctcct agtggtccat aaaaactccg agaagaagag
     8821 cctgaggcaa acttggcttc ctgcgctaac tgacttctat ccaatccaag cttgaaataa
     8881 gcattacaag ccttgaccag ggcggacaaa ccactagaac ttgaggacag gcccgctgcc
     8941 gtaggcatat tgttttgagt atcgatacgg acaaagccct caccagctgg acgataacgg
     9001 tcaataatct tactcatctt ggcatgctcg acctcatttt gtagctgacc attgatgtaa
     9061 aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg tcgtctctgt atacatattt
     9121 tccaaagtta gagaaatact gctagtagca ggcaccatct ctttttcttt tttctttccc
     9181 caatatttga taatagcaat atttgcgtag gaacgtactg ttacaggctc tctatccatg
     9241 tctgaacagc tcctttctct tctaatcttt ctgctagttc ttgtgcgtgt gtcaaattgg
     9301 ttaccaaggc tatgatacaa cctcctagcc caccaccgct catcttggca cccagagcac
     9361 catggctaag agtcgtttca accaaaaagt ctgcctcagg gctactgact ccaatttctt
     9421 ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc ttcagcatct ttttgtgaaa
     9481 tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg caaaaacggt agggcatcct
     9541 tgcccttatt ttgaaccact tggatggctt cacgagtatg accataaaca cccgtatcgg
     9601 caatcaccaa ataggcggat aaatccatct caagttctgt aaatcctacg ttcttgataa
     9661 agcgaatagg ttggtcactg agacaggtct tagcatccaa accactagga ttcatatggg
     9721 caatcatttc agctcgattg accaagattt ctagtacatc atgaggcaga acagcctgat
     9781 agtagtcaaa taccgcacga atggccgcta tgctgatagc cgctgacgaa cccatccccc
     9841 gtttctcagg gatagccgag tcaatctcac aacgaatgca ggcttctgtg atatccaaat
     9901 actccagtga ggcataaacc gccatggaca aggtatcctc ctcataaagg cgccaaggac
     9961 tctctgcagg aactacctta caggtcacct ccacctccaa aagaggcagg gaaatggcag
    10021 gataaccgta aacgaccgca tgttccccta ttaaaattat cttactatgt gcctggccga
    10081 caccaacttt ttttgtcatt ttttcctttt actagacgaa aaaacgtctt atttttcata
    10141 caaatattaa ttctttccta tctattttat tatattttca caaaaaaagc gattgtttcc
    10201 attcacaatc gcttctttca ttattgaacc cattcgccat tataattgac agaatagcca
    10261 tctacggtcg tattcactgc caaggcacct gagctataag cgtagtacca tctgccattg
    10321 acctggaacc aacctgtcgt catagaacga cgaaagaaac tccataccat taagtaaaga
    10381 ggaaagtcgt gagggagcat gcgccattga caacctgttt tagtgacgta caaagtatca
    10441 ttaacaagta ctcgtttcgg ccatttatag gtgcggtgtt tggagaaata gggttcaatc
    10501 ttcgcccatt cttgatcgtt taaatcagta tcatatgctt tacgtatcat aactctagtt
    10561 taacattttt ttgtgaatac aggttctaaa taatcgacca cgaaaatttc ttaagtggaa
    10621 aacgccttat gaagtatgct acgggaaagt tatgcactta atttgacaat tca
//
LOCUS       DAYYQN010000063        10460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYYQN010000063 DAYYQN010000000
VERSION     DAYYQN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.064"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..432)
                     /locus_tag="ACHBRQ_001712"
     CDS             complement(<1..432)
                     /locus_tag="ACHBRQ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593334.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGI"
     gene            complement(432..1196)
                     /locus_tag="ACHBRQ_001713"
     CDS             complement(432..1196)
                     /locus_tag="ACHBRQ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593335.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LLIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKRKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1292..1414)
                     /locus_tag="ACHBRQ_001714"
     CDS             complement(1292..1414)
                     /locus_tag="ACHBRQ_001714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593336.1"
                     /translation="MYYNGGGDNYIGSGTRLAMAPQFGNHVRIHTSGSWSPDSY"
     gene            complement(1875..2507)
                     /gene="pyrE"
                     /locus_tag="ACHBRQ_001715"
     CDS             complement(1875..2507)
                     /gene="pyrE"
                     /locus_tag="ACHBRQ_001715"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM9593337.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2541..3242)
                     /gene="pyrF"
                     /locus_tag="ACHBRQ_001716"
     CDS             complement(2541..3242)
                     /gene="pyrF"
                     /locus_tag="ACHBRQ_001716"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9593338.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3475..4695)
                     /gene="divIB"
                     /locus_tag="ACHBRQ_001717"
     CDS             complement(3475..4695)
                     /gene="divIB"
                     /locus_tag="ACHBRQ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HFM9593339.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEE
                     ESNRNQTNQRSSRR"
     gene            complement(4705..5763)
                     /locus_tag="ACHBRQ_001718"
     CDS             complement(4705..5763)
                     /locus_tag="ACHBRQ_001718"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HFM9593340.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5767..7119)
                     /gene="murD"
                     /locus_tag="ACHBRQ_001719"
     CDS             complement(5767..7119)
                     /gene="murD"
                     /locus_tag="ACHBRQ_001719"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9593341.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(7223..7864)
                     /locus_tag="ACHBRQ_001720"
     CDS             complement(7223..7864)
                     /locus_tag="ACHBRQ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9593342.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7866..9887)
                     /locus_tag="ACHBRQ_001721"
                     /pseudo
     CDS             complement(7866..9887)
                     /locus_tag="ACHBRQ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(9970..10269)
                     /locus_tag="ACHBRQ_001722"
                     /pseudo
     CDS             complement(9970..10269)
                     /locus_tag="ACHBRQ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         3197 a         2277 c         1822 g         3164 t
ORIGIN      
        1 tataccataa aattctctta aaaaatcaac ctcctttatc tccaaagaga agctaaaacc
       61 attactaaat gcaattagaa aaatcaataa aaataaagtc gccgtccaaa tccccgtact
      121 aagagctgct aatttgaaac taaaactggt aaagtgctta attgatttca gacgaatgcg
      181 acactccaac ctattaaaat agttattcat caaataaaaa aagaacaata tatatgtgaa
      241 cggaaagcaa tatactccag tcgtcatatc ttgaagtaaa actaaaatcc attctaatac
      301 atttggatgg attgaatatt ggcgacagcg caataaatat actgtactag ataaaacaca
      361 ggatagcagt aatataaaat aaaccaatac tgataaaaaa tcttttttgt aaaattgaac
      421 aaattgtttc attatacata gtcctctgaa tgtagaaaaa atgtaccata aacaaccaaa
      481 caactaacaa ataaaataaa agcaagatgc ccactaacta aggaaagact gatatctttc
      541 tgatatccca aagctaatgt tgtcacaggt tctaagtaag atagccctaa aatagcccaa
      601 aaaataccac caaccatcat ataggcaact gggatgaaaa tagctcctat ttttctcttc
      661 actagcaaag cactagctag tccaaaaata gagaacacag cgccccaaac tccataccag
      721 agagtcgtca caagactata gagcaactga ttagaatcaa ataattcttt taaggcacca
      781 ctataatctc caatataaat ttcctgataa ggagtcacta aaagattaat tcctaataat
      841 aataaatagg ggagaaaaaa gactagaaaa gaagaaataa ttgcagtact acctacaata
      901 gccagatact tctttttaga aattcggcac aattgtgctg ttagaaaatg actctcagca
      961 tcctctatta tctgactaga atagggcagt gtacagataa gtgcagctac taggctaatc
     1021 ggtgaaaatc ctcgaataga agaagcggca aaaaaaatca ataaaccttc aattttataa
     1081 ataccattga aagcaaggaa atttcctaaa ctcatgcaaa gaagggtcaa aaataaagac
     1141 atataaaatc gaggtgattg aacgactcgt acaagattac aaatgaaaaa ttccatcctt
     1201 actcctcctt ataataaaaa tagggtgtag cattcttttt tcatgctaca cccacaatca
     1261 accatcttta aggcttactc tgacaagtaa gttaataaga atctggactc caagaacctg
     1321 aagtatgaat tcttacatga tttccaaatt gtggcgccat agctaatcta gtaccagaac
     1381 caatataatt gtcaccacct ccattatagt acatgacaat cctagagcca gaccccaatg
     1441 aatataccgg ggtaatatct gacccactat aggcgctacg aatagaggta tttaaccttt
     1501 taccgccacc agtgctgtca ctattattaa ttccagcaga ggcgttttct ttctcaagct
     1561 cactagttgt atccgaaccc caacgcggca acgttaaatc ataatttcca atctgtctag
     1621 ctaaaactgt agggagaaac aaactagatg caattgtaac tacgcaaaaa aggaatatat
     1681 ttttcttctt ttcatgacat cgtcctctta agaagtgata aacaagatag ttgattgatc
     1741 aatttgatac atattacatt ttcattatat caataaaaga aagcgttgtc aattatttta
     1801 tttaaaatat tgtcatattt taaataaaat ggtaaaagag actcggtagc ctcgtctctc
     1861 tttatcttac tgacctaacc ttcttgccaa ttttcttggt cttctttaaa gcgttttaga
     1921 agatcaaggc cctctggcgt gatgtaacct tcttcttggg ctagatggat aagctcgcta
     1981 tagtttgaaa gcgtcacaag tttaacacca gcatctgcaa agttcttatc tgcttttggc
     2041 aattggtagc tgaaaatcgc tacaactcca agtacatctg ctccttctcg cttggctgct
     2101 gctacagctt caagaactga accacccgtt gaaataaggt cttcaaccac taccattttt
     2161 tgaccttgag ctacgcgacc ttcgatttga ttaccagctc cgtggtcttt tggtttgcta
     2221 cggatgtagg caaaaggcaa gtccatctta tcagcaataa tggctccgtg tggaatccct
     2281 gctgttgcag ttcctgcaat cacttctact tcaggaaagg cttctttgat agcttccaca
     2341 aaaccatttt caattagggt acgagtttct ggataggcta gtgtcacacg attatcagtg
     2401 taaatcggtg acttgatacc agatgcccaa gtgaagggtt cctctggttt gaggtaaacg
     2461 gcttggattt tcaagaggtg gctagcgata tctttagcaa gtgtcatggt attctccttt
     2521 tatttttcta atctagttct ttaattccag tcctgtgtcc attcatcctt gatggcatga
     2581 taagctgcaa caggatcctc agcttgggta atgggacgtc ccactacgat atagtcactg
     2641 ccgatttgat aggcatcagc aggtgtcatg actcgttttt gatctccaac tgcaacacca
     2701 gctggacgaa tccctggtgt cagacagata aaatctggat tggtagcctg cttgatgact
     2761 tgtacttcct gagccgagca aacaacacca tccaagccag cttcagctgt cttcttggca
     2821 tagtgaatca cagactcttg cagactggtt tggatatttt gaaactcctg catctgagct
     2881 tctgacgttg atgtgagctg agttacagcg atcaatttgg cttgactccc aagaccttca
     2941 cgcgccgcct tcatcatctc tacaccacca gccgcatgga cattagtcat atcgacacca
     3001 agctgagaca agaccttcat ggctgacttg actgtattag gaatgtcatg aagtttgaga
     3061 tccaaaaaga cactatgacc caaacctttt aagtaggaca caatctcagg ccccgctgcg
     3121 taataaagct ccatccctac cttgagataa aggctttctt ctgctgggaa aagagctaaa
     3181 aattccttga ccgcctcaaa actaggaaaa tcaagagcaa tgattggacg atgttctcgc
     3241 ataggtttct ccttttacct ttactagtga gagagtctgg ccaacagaag ccacgaaaaa
     3301 aggaacctgc caacagcaag gttccacgaa aaatgggtag tctccaccct ttcaccgtct
     3361 aaccttagct gcctctctgg actgctttaa aggtttttta ctattctatt atttctacta
     3421 gcacttgtca agtaaaaaca tggtcactaa agaactataa gagaaaaggt aaacctagcg
     3481 acgcgatgaa cgctgatttg tttgatttcg attgctctct tcctcttgtt ttttctgttc
     3541 ttcttctaaa cgtttctgtt cttcttcctg ttttttctcg gcttccttgg cctcttgttt
     3601 ggctttttcc tcagcttcca taattaattt atccgccaca gtgtaactgt aaattccagc
     3661 ttccatgtca atcacactcg gttctgacaa ttgaggctta attttactgt aatatggcag
     3721 tttcttactc atttcagata gaggaaccaa gacttcgtcc gaatcattca tggtcaatcg
     3781 aattaaatcg gatgtcacct tgcttggggc taattccacc ttttggatag ccgccttgag
     3841 ttctgggcta atttgagcaa gttctgagac aaaaaccttg atttgttcac tatcattaaa
     3901 gagaactgat aaataagttt ctggtaaact gttcaggctc acagaactag tctcaagctg
     3961 accactggaa agaataggat aatgattttc accagaaata tagtaggcta caatatcata
     4021 ttccttgacc ttaatagtga acttagttgg aaattgatag acaagttgag ctgattcaac
     4081 ccaatagtta gacttaatct gcttttcata ttttgccttg tctagcagaa ggttaatcgt
     4141 ataatccgaa tcctgaatgc ctgaagcctg tcgaatatca tcagctgtag tttgcaccgt
     4201 tccctcaaca cgaatatctt tcatggtcgc ataaggactg agcaagtagg cagagacaat
     4261 caataaaagc agacttggaa ataaaatcgt gaaggctcgc aagatatgga taccaggaat
     4321 ctttgctttg gctggttttt cctttgtagc ctttttagaa agctttttat cctgttcctc
     4381 cttctcttta gactctggtt cttctttctc ttctttctct ttgtcagcct ctgaggatgc
     4441 tactttttct tcagactctt ccttagctga ttctgaatct tcctggtctg tttcactctc
     4501 ctggtcctgt ttatcctctg acttctcaga ttcttctccc attcgagctt gtctttcctt
     4561 ttccttctcc tcagctagag ccgcctcttc ttcagccttc ttttttagat attcttggtt
     4621 tcgtttctgc cattctgata actctttcaa ttcttcgagg atttctttgt cctcattttt
     4681 cttatctttt gacatttact ttccttatga taaatctttt ttcaacaatt gataaaaatc
     4741 tgctagagat ttcaattcct tagaagcttt catcttagct tggtaatctt ccttgtgact
     4801 tagtaagtga gaaagcttct cttccaaact atccaaggtc aaatcgcttt cttgaaggtc
     4861 ttctgcatag ccttttttta caaagtaagc tgcattttca atctggtcac cacgactagc
     4921 ttcacgacca agcggcacaa tgacatgcaa ttttgctatc gccaagagct caaaaatcgt
     4981 attggcacca cctcgtgtca caacaatatc agccaattcc aacaagggtt gatagagatc
     5041 ggtcacatag tcaacacgaa aaagattttg gctcaactca ttcaggctag aatctccagt
     5101 tagattgata atattgtagc gctctgttag ttctttctta tggtctgtca ccaattggtt
     5161 aaagacacga gcacctgcag aaccgccaac aaacaataca gtcggcaatt tatgattaaa
     5221 gtgggtttga atatccacca attcatctgg ttctggattt ttttgatctg aaaccttggt
     5281 cactgctccc acatgctcaa ccttagacaa actcgaagct tgctcaaagg ttgaatacat
     5341 cttagtcgca aatttatagg cgattttatt ggccaagccc atagacaggt cagattcgtg
     5401 aataaagaca ggcactcctg acacacgcgc tgcgataaca ggcggtactg agacaaagcc
     5461 cccctttgaa aaaagggtct gtggacgaag tcgcaacatg ataaagagcg attggacgat
     5521 tccccagcca actttgaaga cgtccagcat attttgccaa gagaaatagc gacgcaattt
     5581 cccagtcgca atggagtgga aagtgacatc caaacctgac ttaaggattt cttggtgttc
     5641 gataccacgc ttgtccccga tatagtggac ttcccaacca tcttcgatga acttgggcat
     5701 taacaaaaga ttgagggtaa cgtgtccaac cgtcccccca cctgtaaaga caattttttt
     5761 catattttat tcttttaact ccgctactgt gtcgataaag aggtcgccac gtacttcaaa
     5821 gttagcatac atatcccagc tggcattggc aggactaaga agaaccacat ctccttgagt
     5881 cgcaagctca taggccttgc gggtcgcatc tgcaatatct gtcgcctcca cataagcgac
     5941 accagccttg tctgctgccc gtttgacacg ttctgcagat tgacccagga tgaccatctt
     6001 cttgagtcca gtaatgtctg gcaccaattc gtcaaactca ttgccacggt ccaaaccacc
     6061 tgcaatcaag acgaccttgc tgttgtcaaa tcctgataag gctttttgag tagccaagat
     6121 attagttgat ttactgtcgt tatagaattt aacacccttg atgtcatcca caaactggag
     6181 acggtgtttg acaccaccga aggctgaaag agtttccttg atggtttgat tgtccacatc
     6241 acgaagcttg gctacagcaa tagtcgcaag ggcattttcc acattgtggc tacctggaac
     6301 accgatttca ttcgctgcca tgactacttc accacggaag tagagttgac catcttccag
     6361 ataagctcca tcaacctttt caagtgttga aaatggtaca acagtggctt ctgtcttgga
     6421 agccaagtct tttaccaagt cttgattaaa gttcaagaca aggaaatcag ctgctgtcat
     6481 cttgttctgg atattccact tggctgctac atattcctca aataatccat gataatcgat
     6541 atgagttggc atgaggttgg taataaccgc aatctctgga tggaattctt gaacacccat
     6601 gagttggaaa gaagaaagtt ccataacaag cgtgtcctta tctgatgcta tttgagcaac
     6661 ctgactggca ggatagccga tattccctga taaaagacca tgttgcccag cagcagtcaa
     6721 aacttcccca atcatagtcg ttgtggttgt cttaccgttc gatcctgtga taccaataat
     6781 cggtgcttct gaaatcaaat aagccaattc cacctcagtc aagactggaa ttcccttggc
     6841 caaagccttt tcaatcatgg gattgctgta ggggatacct ggatttttta ccatgagggc
     6901 aaactcttca tccaagagtt ccaaaggatg gccacctgta atgaccttga tcccttcttc
     6961 cagcaaacat tgggcagctg gattgtcctc gaatggtttc ccatcattta ctgtcacaat
     7021 ggcacctaac ttgtccaaca aacgagccgc agattcacca gacttggcca aacctaaaac
     7081 aagaactttc ttatttttaa attgatctat tactttcatg tctcgaactc catttctact
     7141 cctactattt taccattttt atggaaataa aaaagccaca aagtgtgttt gtgactcttt
     7201 cttctaactg aatcttacca tatcatctat gtgataaatc ggtaactcga atgacctgat
     7261 ccacttgctc ccaaatcaga ggattgtggg tcgcaataat aatggtccga ttcggatttt
     7321 ttaaagattc taggatggaa agtaattcct cagaattttt ggggtctaag gaagcggttg
     7381 gttcatctgc aagaatcaaa ggcggatcct ttaaaattat cttcgctagt gcaacacgtt
     7441 gtgcttctcc tcctgataac tcaaatatag gttgcttcaa atccaaataa gagaggttta
     7501 cacagtttag agcttgtttc atcaaagaga ttttctcttt ttccttcaac tttttaccaa
     7561 ctaaacccag attgagattc tctttgacgg tttggctttc aattaagcca aaatcttgaa
     7621 ataagtatcc taagtaatct ctaaagaaaa cagaaggctt gatgtcctta agagaagtgc
     7681 catcatagat gatttgccct ttgtcatatg gctccaatcg tccaatcata ttcaagagtg
     7741 ttgtcttacc acagccactt gtaccgatta aggcataaat tttcccacct tcaaaatgaa
     7801 gattcgtatc tgaaaatagc tgacggcttc caaatttttt agatatattc tttagttcaa
     7861 tcatcctatt ttcctttcat aattgtcata gaaacacgag attctttctg cgcttgacgg
     7921 taaagcgtca aaactgcact agctagaaag accaataaag tgagcaagcc aatcaccaag
     7981 tctcgactgc ttaaaataaa gagactagca ccaaatacaa aactggcaaa ttggctaacc
     8041 atatactgag catgtgtttc aaaaaatcgt aaacctgaaa ttcgtttaat caagatatct
     8101 cggcggaatt gctcgaaata tagaagattg acagaataaa atagtaataa ggaactagct
     8161 atccccacaa tagctcctaa gattaaagtt gctgtttcag tttgaacttc attataacga
     8221 gttagataaa cacttcttcc ttctttaaga taggatactt gctcataaat tccagctttc
     8281 ttcaagagtt ctagcccact ctcatatcct ttgataaaga gttgttttcc agcattgata
     8341 gaccaactag ataaggatat aaaactatca cctgtagaag tcggcgtgaa taccactaaa
     8401 atcggatcag tcaaatactg agtagatacg ggattctcac cgttattata aacaaaccgc
     8461 ttttctccca ttgaaagata actaacgtgc gctttcatct cataatccaa aggagcactt
     8521 gcctcctcac cagattttcc ataataactc aatctttctt caaaaacttt cttaagttct
     8581 gcttctcgag agcgcaaatg ttctgggagc aagaggataa actcaccttt ttggagatgg
     8641 gctaacttct gtttggtctc agcatctacc acgacctttt ccttgtccaa ataactggga
     8701 ctaacataga gcgtattagc atctggacta taggtatcca gtgtctctcc ctgttcattt
     8761 tttccttgtg gattggcaaa atggagcaga ttatccttta cataaagagc ttgttcctct
     8821 tcgattgctt ccttggcaaa ggcataccac ttgctctgat tttctgtatc ttttcctcta
     8881 tcacctaagc caaaagaaat ttgataatag tctgctctat cctgccatgc ttgttttgaa
     8941 atttcaagtt ctttcaatcg ttggtaagac gtcaaacctg tcttaacagc gtatcctact
     9001 gtaaagacag caaccaactg acacaatagg gttaaaatca tcaggcgttt aaggggtaat
     9061 ctccccttaa taacgggaac taatgctttg taactcaaac tcattagata aagaagcatt
     9121 agtaaaattg aaatcgccaa taaaaacaac agatagaaac taatcccaaa accataggtg
     9181 gctaacaaga taggataaaa caaaccttga ctaaaaagaa cgactccccc acctaggaag
     9241 gaaaggaggg ctgatagaag gagctatttg atatcagtag ataaagaatg ccccatgatg
     9301 gataagagag tctgaccaga aaagagtttt atacctgctg ctctcatttc cttaatccga
     9361 gtgataatca ctaaagcaaa gaaagataag ccaaatattg ctaaactaat taaaataagg
     9421 ggatttagta atattcgaaa agcaagaaaa tagggcggta tctttcggtc agcacttgct
     9481 ttataaccca aatctcctaa tttatcggca agcttttctt tcgtcaagga gcctgacaaa
     9541 aggagataac tatttagcgg atcactacgt tcacgacttt cttggctagc ttcttggaat
     9601 tcttttggta aagttccctg accataagtt gcataagtaa agtgagtcgt cccatcctta
     9661 ctcggctcta caattcttct agctattaaa ctctgttctg agtttgcaaa attctccaat
     9721 tcctgttcaa atacctcacg cgtcggttcc tgagtatctt ttttgacacg aagtaaagaa
     9781 acggaatcat agcttgcata taaatattgt ggcgcacgta agacaataat ccaagcaagg
     9841 aagaagctga gaaaaaaagt tgataataat atgaatagtt tcttcatagt agactccttg
     9901 gtagactcct tgtaaacaaa attccccctg taatttctta caaggggaac gatttaaatc
     9961 aatgaacgat tagtcataat cacagtaaaa tgctacttgt tctccccatt tagtccaaat
    10021 ccatgcatag gaggtttttc cagcaggagc ttctccttta tcagaatgac catcccatct
    10081 gctcacgatg gatgaataat gatatttttt accatgatag taatttgaaa aagcctaacc
    10141 acctcctgaa ccttctccat atgtccatac tcctccatct ggatattata cagcagctga
    10201 tgcagctccc aataatgtaa aacttgaaat aagagctaga gcaagtaatc tatgtttttt
    10261 cgttttcatt ttattttttc tttcaaaaaa atcacacctt gagcaacaat gcaacaaaat
    10321 aaatcctcct ctctctttta ttgaaaccgc tttcttatgt gataagaata acatttttat
    10381 tatttgttgt caaggaaaaa atcgaatttt ttagatattt tactatatta cctctatgaa
    10441 taatattata tagtagtttt
//
LOCUS       DAYYQN010000064        10424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYYQN010000064 DAYYQN010000000
VERSION     DAYYQN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.065"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..389
                     /locus_tag="ACHBRQ_001723"
                     /pseudo
     CDS             1..389
                     /locus_tag="ACHBRQ_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            475..687
                     /locus_tag="ACHBRQ_001724"
                     /pseudo
     CDS             475..687
                     /locus_tag="ACHBRQ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..1033
                     /locus_tag="ACHBRQ_001725"
     CDS             587..1033
                     /locus_tag="ACHBRQ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9593343.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1171..2475)
                     /gene="eno"
                     /locus_tag="ACHBRQ_001726"
     CDS             complement(1171..2475)
                     /gene="eno"
                     /locus_tag="ACHBRQ_001726"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HFM9593344.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2639..3085
                     /locus_tag="ACHBRQ_001727"
     CDS             2639..3085
                     /locus_tag="ACHBRQ_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9593345.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3082..3885)
                     /locus_tag="ACHBRQ_001728"
     CDS             complement(3082..3885)
                     /locus_tag="ACHBRQ_001728"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9593346.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3894..4223)
                     /locus_tag="ACHBRQ_001729"
     CDS             complement(3894..4223)
                     /locus_tag="ACHBRQ_001729"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM9593347.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4168..4539)
                     /locus_tag="ACHBRQ_001730"
     CDS             complement(4168..4539)
                     /locus_tag="ACHBRQ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HFM9593348.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="ACHBRQ_001731"
     CDS             complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="ACHBRQ_001731"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFM9593349.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="ACHBRQ_001732"
     CDS             complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="ACHBRQ_001732"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFM9593350.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7157..8299)
                     /locus_tag="ACHBRQ_001733"
     CDS             complement(7157..8299)
                     /locus_tag="ACHBRQ_001733"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFM9593351.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="ACHBRQ_001734"
     CDS             complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="ACHBRQ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HFM9593352.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3194 a         2293 c         1831 g         3106 t
ORIGIN      
        1 caccaagtaa aaggaacaaa accaagaaaa gttgatagag atagacttaa aaactatctt
       61 actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg tcatccaact
      121 accatccact atgcgctcaa agctatgggc tacactcgaa aaaaaagaac cacacctact
      181 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      241 tagcacaggt ctaacaaatg aatggtgagt taatcgctcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaaatttct cttaccaaca ttaaccgcac
      361 cattggttat tatattaaaa cgaagtaaga ataggacaaa tcgatcagga cagtaaaatc
      421 taattctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta
      481 atggacaatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      541 cataaacttt tacctcttcc tccctactcg cctgagtaca atcttattga gaaaacatgg
      601 gctcatatca aaaagcacct caaaagggta ttaccaagtt gcaatacctt ttatgaggct
      661 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      721 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      781 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      841 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      901 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      961 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
     1021 aaagattttt taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg
     1081 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
     1141 gttcgggata aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa
     1201 tccacgatat tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt
     1261 agcgatgcgg tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac
     1321 tgcaatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt
     1381 gtaaccagct tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg
     1441 gttaactttg ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc
     1501 agtgtttgtt acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt
     1561 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1621 gtatttgtta accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc
     1681 accttcacct tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga
     1741 tgaagcacag tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat
     1801 cgcagcaagg atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc
     1861 ttcgtcacct acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa
     1921 gatttcagca ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat
     1981 gaactcttgg aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat
     2041 tggagttgga agaactttag tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag
     2101 gtagtcagca gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa
     2161 tttacctttg ttaggagtac cgtcaagtgc aatcatagca cggtcaatag cttgttgatc
     2221 acgtacatcg tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt
     2281 ttgtgtacca agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg
     2341 ttcaccagta gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta
     2401 aacttctact tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc
     2461 agtaataatt gacatttttt actctcctta tgagttaaat tttttacacc tctataatac
     2521 cttaaaaccc ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt
     2581 ataaagtaat cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat
     2641 gacagattta tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga
     2701 tgagcaaaga cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga
     2761 cacagcaaat agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga
     2821 aaaagcaaag ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt
     2881 ctacttaaaa gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat
     2941 tacttctcct tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga
     3001 ccttcgactt gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc
     3061 cttatggaag aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc
     3121 agccgtgcgc tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt
     3181 ctccaaaata gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc
     3241 aagactaccg caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat
     3301 aggcgctttc ccagcaaagc tttgactatc aagtcttcct tctccaacaa caaccaagtc
     3361 agcatctgaa actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac
     3421 gatacttgcc tgagcaaagg cacacaaacc agcagcaatc cccagtcctc catcattact
     3481 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga gatggagaat
     3541 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     3601 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     3661 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     3721 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     3781 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     3841 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     3901 aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     3961 tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga cttcaacatt
     4021 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     4081 tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg tccaactaat
     4141 tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata ggcaatacca
     4201 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     4261 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     4321 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     4381 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     4441 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     4501 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     4561 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     4621 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     4681 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     4741 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     4801 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     4861 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     4921 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgctg
     4981 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     5041 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     5101 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     5161 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     5221 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     5281 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     5341 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     5401 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     5461 tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt ccttctgaaa
     5521 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc tcataggctt
     5581 gaatccaaca gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     5641 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     5701 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     5761 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     5821 actgtctacg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     5881 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     5941 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     6001 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     6061 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccaa gctcaacttc
     6121 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     6181 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     6241 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     6301 agatgtatta gcatagtaag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     6361 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatattcg cttgataata
     6421 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcaa aagctccctc
     6481 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     6541 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     6601 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     6661 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     6721 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     6781 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     6841 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     6901 tcctagatag taatggctaa gaagggttga taagccccac tcgcgccctg aacgaatatg
     6961 gtcaaagact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     7021 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     7081 tgctactgga cggtggtccg tcaatgtcga catatcgtga aaaccaactg tatttcctaa
     7141 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     7201 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     7261 caataatggc acgtttaatc ttagctcctt gaccaatgat agctccactc atgataactg
     7321 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     7381 cagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     7441 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     7501 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     7561 taccaacatc tttccaataa ccactaaatt cataagcata aacactttca cctgactcaa
     7621 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     7681 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     7741 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     7801 tcatgatacc aaaacggctt gcttctttaa gagggacgtc taaaactgct actgtcaagc
     7861 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtgat
     7921 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     7981 aaatagcgtg actagtcccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     8041 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     8101 tgttgagagc aagtggttga tactgtgtaa cgaccccaac attatgaatc cctgagttgg
     8161 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     8221 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     8281 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     8341 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     8401 aaggtctgct cataatcttt ccatagtcct tcttgcgttt gaacagtttg attatgttct
     8461 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     8521 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa gatacagact
     8581 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
     8641 gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca gcgatgatct
     8701 ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat tgggtcttct
     8761 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
     8821 cccatgaaga gcaatttctt accaggatga caaatttggt acgtatagag attgcgcaag
     8881 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
     8941 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
     9001 aaagtcacca ggttaaagtc atatttacga tagatcggat cttcttcgta gaaacggagg
     9061 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
     9121 attcccgtaa tcttggtcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
     9181 tctaacttaa taacctcatt caagcgctga aggaaataat aaccttcata gttgagattt
     9241 ccgccatctt tattaggtgt ccatggagca tcatcatagt ccaaatagag catgttgcta
     9301 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
     9361 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
     9421 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
     9481 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
     9541 gtatgacact cctcgacaaa atcttgaaac tcctctggtc ggccataagc atgctctaaa
     9601 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
     9661 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
     9721 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
     9781 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
     9841 tccttccatt tcttctcacg aagctctgtt aagattgccc ctgttcctgg acgagcctca
     9901 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
     9961 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    10021 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    10081 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    10141 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    10201 gcttctctat tatccatata ctcctcttct cctgtaagcg ttttctatgt ttttattata
    10261 ctaccttttt agagaagatt caagtaaatt actatacttc tttaattatt ttgaaaatct
    10321 acaacaagtt cacttactcg ttcaattgta aatcaatatt ttttcaaaaa attgcgaaaa
    10381 ctcctttctt tttctactat agtgaaatga aataaaacat gcgc
//
LOCUS       DAYYQN010000065        10097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYYQN010000065 DAYYQN010000000
VERSION     DAYYQN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.066"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            68..199
                     /locus_tag="ACHBRQ_001735"
     CDS             68..199
                     /locus_tag="ACHBRQ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9593353.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            220..1770
                     /gene="dltA"
                     /locus_tag="ACHBRQ_001736"
     CDS             220..1770
                     /gene="dltA"
                     /locus_tag="ACHBRQ_001736"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9593354.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPRLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            1767..3011
                     /gene="dltB"
                     /locus_tag="ACHBRQ_001737"
     CDS             1767..3011
                     /gene="dltB"
                     /locus_tag="ACHBRQ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9593355.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGIVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            3025..3264
                     /gene="dltC"
                     /locus_tag="ACHBRQ_001738"
     CDS             3025..3264
                     /gene="dltC"
                     /locus_tag="ACHBRQ_001738"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9593356.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            3257..4525
                     /gene="dltD"
                     /locus_tag="ACHBRQ_001739"
     CDS             3257..4525
                     /gene="dltD"
                     /locus_tag="ACHBRQ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9593357.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            4680..5120
                     /gene="adcR"
                     /locus_tag="ACHBRQ_001740"
     CDS             4680..5120
                     /gene="adcR"
                     /locus_tag="ACHBRQ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HFM9593358.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            5120..5824
                     /locus_tag="ACHBRQ_001741"
     CDS             5120..5824
                     /locus_tag="ACHBRQ_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9593359.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            5817..6623
                     /locus_tag="ACHBRQ_001742"
     CDS             5817..6623
                     /locus_tag="ACHBRQ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9593360.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            6633..8138
                     /gene="adcA"
                     /locus_tag="ACHBRQ_001743"
     CDS             6633..8138
                     /gene="adcA"
                     /locus_tag="ACHBRQ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HFM9593361.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQSLDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(9267..10040)
                     /locus_tag="ACHBRQ_001744"
     CDS             complement(9267..10040)
                     /locus_tag="ACHBRQ_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9593362.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ"
BASE COUNT         2926 a         1947 c         2163 g         3061 t
ORIGIN      
        1 gaaaaataag ctagtaagaa tcactcttat tttagcgaaa aattacagaa atgaatggtt
       61 tttcttgatg aaacagagaa aagaattgta cctctttctt ggtcggacag ccttgtattt
      121 tcttatcttt ctagggctgc tttacttctt tagctatctt ggtcagggtc aaggaagctt
      181 tatctataat gaattttaac ttgaaggaga atccctattg tgtcaaataa accaatagca
      241 gatatgattg aaaccattga gcattttgct cagacacagc ctagctatcc tgtctataat
      301 gttttggggc aggaacacac ttatggcgat ttaaaggctg attcggatag tttggctgca
      361 gtcattgacc aactaggctt gcctgagaag tctcctgtgg ttgtttttgg tggccaagaa
      421 tatgaaatgt tggcaacctt tgtagcgctg actaagtcag gtcatgccta cattccaatt
      481 gatagccatt cggccttgga gcgagtttca gctattttag aagtagcaga gccaagattg
      541 attattgcca tttcagcctt tcccttggag caggtttcta caccaatgat aaatctagct
      601 caggttcaag aagcctttgc ccaagggaac aactatgaaa tcacgcatcc agtcaaggga
      661 gatgataatt actacattat ctttacttct ggtacgactg gtaagcctaa gggagtgcag
      721 atttcacatg ataatctcct cagctttaca aactggatga ttacggataa ggaatttgcg
      781 acaccgagtc gtccgcaaat gctggcacag ccaccttatt cttttgactt gtctgtcatg
      841 tattgggcac cgaccttggc acttggtggt acgcttttca ctcttccttc agtcatcact
      901 caggacttta agcaactctt tgcggctatc ttttcattgc caatcgctat ctggacatca
      961 acaccatcct ttgcagatat ggccatgttg tctgaatact tcaacagtga gaaaatgcct
     1021 ggaatcacgc atttctactt tgatggtgaa gaattgacgg tcaaaacagc tcaaaaactg
     1081 cgcgagcgtt tcccaaatgc ccgtatcatc aatgcttacg gcccaacaga agcgacagta
     1141 gctctgtcag cagttgccgt gacagacgag atgttagcga ctctcaaacg cctaccaatc
     1201 ggctatacca aggctgattc tccaaccttt atcattgacg aggaaggaaa taaactgcca
     1261 aatggtgagc agggagaaat cattgtttct gggccagctg tttcaaaagg ttatatgaac
     1321 aatcctgaaa aaacagcaga agccttcttt gagtttgaaa atctgccagc ctatcacaca
     1381 ggcgatgtgg gaaccatgac agatgagggc ttgcttctct acggcggacg catggacttc
     1441 cagattaagt ttaacggtta ccgcattgag ttagaagatg tctctcaaaa cctcaacaag
     1501 tctcgcttta tcgaatctgc tgtcgcagta ccgcgctata acaaggacca caaggtacaa
     1561 aatctattgg cttatgtcat cttaaaagac ggtgttcgtg agcagtttga gcgagatatc
     1621 gatattacca aggccatcaa ggaagacctg acagacatca tgatgtccta catgatgcca
     1681 tctaaattcc tttaccgaga cagtttgcca ctgactccaa atggaaagat tgacatcaaa
     1741 ggattgatta acgaggtgaa taagagatga tggagttttt tcaacagctt cctcatttag
     1801 agccatatgg caatcctcag tattttgttt atgtgattgc tgcaaccttg cccatcttta
     1861 taggtctctt tttcaagaaa cgctttgcct ggtatgaagt gttggtaagt ctcttcttta
     1921 ttgtcaccat gttggtgggt ggaaagacca atcaactagc tgccttgggt atttaccttt
     1981 gctgggaaat attgctcctg cttttctaca agcattatcg aaaaagcaag gatggcaagt
     2041 gggtcttcta cttagttagt tttctgtccc tacttccgat tatctttgtc aaggtgcaac
     2101 cagctatcaa tggaacgcag tctttgcttg ggttcttggg aatttcttac cttacctttc
     2161 gttcggttgg aattgtcatc gagctgagag atggagtgat taaggatttt accctctggg
     2221 aattcctccg tttccttctc ttcatgccaa ctttctcgag tggtccaatc gatcgcttta
     2281 agcgatttaa tgaaaattat caggctattc ctgagcgaga tgagttgatg gatatgctgg
     2341 atgaatctgt ccgctatatc atgtggggct ttttgtataa gtttatccta gctcatgttt
     2401 taggagagac cttactacct cctctgaaga atttagcctt gcagtcaggt ggcttcttta
     2461 atctctatgc cttggcagtt atgtatactt ttggtctgga gctcttcttt gactttgcag
     2521 gttattctat gtttgctttg gccatctcaa acttgatggg aatccgtagc cctatcaact
     2581 ttaacaagcc ctttttatca agggatttaa aggagttttg gaatcgctgg catatgagtc
     2641 tgtccttctg gttccgtgac tttgtcttta tgcgaatggt gatggtgtta accagaaaga
     2701 aagtctttaa aaatcgtaat gtaacctcaa gcatggccta cattgtaaat atgctgatta
     2761 tgggattttg gcatggcgtg acctggtact atatcgccta tggactcttt catggactag
     2821 gcttggtcat caatgatgcc tgggttcgca agaaaaaaac gctcaataag gaacggaaaa
     2881 aagcagggaa ggctgcccta cctgagaatc gctggattca gttgcttggt atagttgtca
     2941 ctttccatgt tgtcatgttg tcattcttaa tcttttctgg attcttgaat aatctatggt
     3001 ttaaaaaata aaaggaaata aaatatggat atcaaatcag aagttattga aattattgat
     3061 gagttgttta tggaagatgt ttctgacatg atggatgaag atctttttga tgcaggtgtc
     3121 ttggatagta tgggaacggt tgagttgatt gtggagattg agaaccgttt tgacattcgt
     3181 gtccctgtaa cagagtttgg tcgtgacgac tggaatacag ctaataaaat catagctggt
     3241 attgtggagc tacaaaatgc ttaaacgctt atggatgatc ttcggaccgg tcttgatagc
     3301 tggtttgttg gtttttctgc tcattttctt ttatcctact gagatgcatc ataatctagg
     3361 agctgaaaag cgttcagcag tggctactac tatcgatagt tttaaggagc gaagtcaaaa
     3421 agtcagagca ctatctgatc caaatgtgcg ttttgttccc ttctttggct ctagtgaatg
     3481 gcttcgtttt gacggtgctc atcctgcggt attagctgag aaatacaatc gttcctaccg
     3541 tccttatctt ttaggacagg ggggagctgc atcgcttaac caatattttg gaatgcaaca
     3601 gatgttacca cagctggaga ataaacaagt tgtgtatgtt atctcacctc agtggttcag
     3661 taaaaatggc tatgatccag cagccttcca gcagtatttt aatggagacc agttgactag
     3721 ttttctgaaa catcaatctg gggatcaggc tagtcaatat gcagcgactc gcttactgca
     3781 acagttccca aacgtagcta tgaaggacct ggttcagaag ttggcaagta aagaagaatt
     3841 gtcgacagca gacaatgaaa tgattgaatt attggctcgt tttaatgaac gccaagcctc
     3901 cttttttggt cagttttcgg ttagaggcta tgttaactac gataagcatg tagctaagta
     3961 tttaaaaatc ttgccagacc agttttctta tcaagcaata gaagatgttg tcaaagcaga
     4021 tgctgaaaaa aatacttcca ataatgagat gggaatggaa aattatttct ataatgagca
     4081 gatcaagaag gatttgaaga aattaaagga ttctcagaaa agctttacct atctcaagtc
     4141 gccagagtat aatgacttgc agttggtttt aacacagttt tctaaatcta aggtaaaccc
     4201 gatttttatc attccacctg ttaataaaaa atggatggac tatgctggtc tacgagagga
     4261 tatgtaccaa caaacggtgc agaagattcg ctaccagtta gaaagtcaag gttttaccaa
     4321 tatagcagat ttttctaagg acggcgggga gcctttcttt atgaaggaca ccattcacct
     4381 tggttggttg ggttggttgg cttttgacaa ggcagttgat cctttcctat ccaatcccac
     4441 accagctccg acttaccatc tgaatgagcg ctttttcagc aaagattggg cgacttatga
     4501 tggagatgtc aaagaatttc aatagataat ataatataga agagttcaag aatgaaacta
     4561 gttttcacgt tttttcttga ctcttttttt gcttgtgata taattaactg gtaaacaaaa
     4621 tgtcaaagaa tagtattttt ctcttaaaaa agagaagtct aaaaggaaag gagtcagata
     4681 tgagacagct agcaaaggat atcaatgctt ttttgaatga ggtgattttg caggcggaaa
     4741 atcagcatga aatcctaata ggtcattgca ctagcgaggt ggccctgacc aatactcagg
     4801 agcatatcct tatgctcttg tcagaggaat ctttaacaaa ttcagaattg gcccgtcgtc
     4861 tcaatgtcag tcaggcggca gttaccaagg ccattaagtc tttggtcaag gaagggatgt
     4921 tggaaacatc taaagattct aaagatgcgc gtgtgatttt ttatcagttg actgacttgg
     4981 ctcgtccaat cgctgaggag caccaccatc accatgagca tacactttta acctatgaac
     5041 aagtggcgac tcagtttact ccaaatgaac aaaaagtgat tcagcggttt ttgactgctt
     5101 tagtaggaga aatcaaataa tgagatatat tacggtagag gatttgtcct tctattatga
     5161 taaggagcct gttcttgaac atatcaatta tagtgttgat agtggggaat ttgttacctt
     5221 gactggggaa aatggagcgg ctaagacgac gctcatcaag gccagtcttg gaattctgca
     5281 accacgcatt ggaaaggtgg ctatttcaaa gacaaatacg caaggtaaga aattgagaat
     5341 agcctatctt cctcaacaaa ttgccagttt taatgctggt tttccaagta cggtctatga
     5401 atttgtcaag tcgggtcgct atccgagaaa aggctggttc cgtcgtttga atgctcatga
     5461 tgaggagcat atcaaggcta gtctggactc agttggcatg tgggaacatc gagacaaacg
     5521 cttggggtct ctatctgggg gacaaaagca gcgagcggtg attgcgcgta tgtttgcttc
     5581 tgaccctgat gtgtttatcc tagacgagcc gacaacgggg atggatgcag gaagtaaaaa
     5641 tgaattttac gaactcatgc accacagcgc ccatcatcat ggcaaggctg ttttgatgat
     5701 tacccatgac cctgaagaag ttaaggatta tgcggatcgc aatattcatc tagtccgtaa
     5761 ccaagactcg ccatggcgtt gtttcaatgt tcatgagaat ggccaggagg tgggccatgc
     5821 ttagtttatt atcttacgac tttatacaac gcgccttttt ggcggttatt gctatgagtc
     5881 ttttctcacc ggtattggga accttcctca tcttgcgtcg tcagagtttg atgagtgata
     5941 cccttagcca cgtctcactt tcaggtgtag cctttggtct ggttttgggg atttctccaa
     6001 ctgtttctac tattgccatt gtcttgattg cggcggtctt tctggagtat ctccgtacgg
     6061 tttacaagag ctttatggaa atcgggacag ctatcctcat gtcaacaggt ctggctgttt
     6121 ctctgattgt catgagcaag ggtaaaagct cgagttcaat gagtttggac caatatctct
     6181 ttggttcgat cgtgactatc agtgaagaac aggtcatttc cctctttgtc attgcggcgg
     6241 ttgttttgat tttgaccttt ctctttcttc gtcctatgta tatcttaact tttgacgaag
     6301 atacggcctt tgtggatggc ttgccagttc gtaccatgtc cattcttttt aacatggtga
     6361 caggggtggc tattgccctt atgattcctg cagcaggagc tcttctggta tcgaccatta
     6421 tggtcttgcc agctagtatt gccctgcgtc tggggaaaaa ctttaaatcg gttatgctgc
     6481 ttgccagtgc gattggcttt ttgggaatgg tagcaggact ttacatttcc tactatgcag
     6541 aaacacctgc aagtgcaagt attaccatta tttttgtaac tgtctttata ctaatcagtt
     6601 tagtaagacg ttttatcaaa taggagacga acgtgaaaaa aattagctta ttactagcca
     6661 gtctatgtgc cttgttttta gtggcttgtt ccaatcaaaa acaggcagat ggtaaactca
     6721 atatcgtgac aaccttttac cctgtctatg aatttaccaa gcaagtcgca ggagatacgg
     6781 ctaatgtaga actcctaatc ggtgctggga cagaacctca tgaatacgaa ccatctgcca
     6841 aggcagttgc caaaatccaa gatgcagata ccttcgttta tgaaaatgaa aacatggaaa
     6901 catgggtacc taaattgcta gataccttgg ataagaaaaa agtgaaaacc atcaaggcga
     6961 caggcgatat gttgctcttg ccaggtggcg aggaagaaga gggagaccat gaccatggag
     7021 aagaaggtca tcaccatgag tttgaccccc atgtttggtt atcaccagtt cgtgccatta
     7081 aactagtaga gcacatccgc gacagcttgt cagcagatta tcctgataaa aaagagacct
     7141 ttgagaagaa tgcagctgcc tatatcgaaa aattgcaatc cttggataag gcttacgcag
     7201 aaggcttgtc tcaagcaaaa caaaagagct ttgtgactca acacgcagcc tttaactatc
     7261 ttgccttgga ctatggactc aaacaagtcg caatctcagg cctttctcca gatgcagagc
     7321 catcagctgc tcgcttggca gaattgacag agtatgtcaa gaaaaataaa atcgcctata
     7381 tctattttga agaaaatgcc tcacaagccc ttgctaacac actttcaaaa gaagcaggtg
     7441 tcaaaactga tgtcctcaat cctttagaaa gtctgacaga agaggacacc aaggctggag
     7501 aaaattacat ctctatcatg gagaaaaacc tcaaggcttt gaaacaaaca acagaccaag
     7561 aaggcccagc aatcgagcct gaaaaggcag aggataccaa gacagtccaa aatggttact
     7621 tcgaggatgc agctgtcaag gaccgcacct tgagtgacta tgcaggtaac tggcaatcag
     7681 tttatccttt ccttgaagac ggcacgtttg accaagtctt tgactacaag gctaagttga
     7741 ctggtaagat gacccaggct gagtacaagg cttactatac aaaaggctat cagacagatg
     7801 tgactaagat taacattact gataatacta tggaatttgt tcaaggtgga caaagcaaga
     7861 aatatactta caagtatgtc ggtaagaaaa ttttgactta caagaaaggc aatcgtggcg
     7921 tgcgtttcct ctttgaagcc acagatgctg acgctggaca attcaagtat gttcagttta
     7981 gtgaccacaa tgttgcccca gttaaggcag aacatttcca tatctttttt ggaggtacaa
     8041 gccaagaagc tctatttgaa gaaatggata attggccaac ctactaccca gataacctat
     8101 ctggccaaga aatcgctcaa gaaatgttgg cgcattaatg agaaatcgac tagttcataa
     8161 gagctagtcg gtttttgggt actagatggc taacttcatt atagactttt cagaactaag
     8221 gaactttaaa aaaataaata aaactcttga aatatctgga cttatatgct agaatgtagt
     8281 tagtgtataa ggttcattag aacaccaaag cccctaacta gtgccatagt taagggcttt
     8341 tttgacgtat cttagcgatt taacgggtgt ttataggcta gtcagtcggt ctacttctta
     8401 ccatctagcc atttgcagac gaaatacaag ataattccag ctataacatc tgctataata
     8461 gtaagagcaa gctctggtat aaggctcatt agtttcacct cctctcacga accgtaagga
     8521 aggtaatcgg taaccgatga caaaagtatt ataccacact aaatttagat catcaacctc
     8581 acttaatgct tgaactattg tagattatat gctagaatgt agttaattga taaggttcat
     8641 tagaacacca aagcccctaa ctatggccgt agttaagggc ttttttgacg tatcttagcg
     8701 atttaacggg tgtttatagg ctagtcactc ggtctactcc ttactatcta gccatctgca
     8761 gacgaaatac aagataattc cagctataac atccgctata atagtaagag cgagctctat
     8821 gataaggctc attagtttca cctcctctca cgaacccata ggaacgtaat cggtaaccga
     8881 tgacaaaaat agtataccac aatacattta gatcatcaag gtcacttaat tcttgaaata
     8941 tagggtctag gcgacaaagt ctttaaaatc agaaaaacgc ataatatcag gtgtgcaaaa
     9001 acttgatact atgcgtttta ttgtgggaag gtttactcca ttttctcctg aaattgagtt
     9061 tttgtccagc ctctgttttt agaattgcta agaaaataat gtcatgtggt gaatatttgt
     9121 aaatcagtca gcagacagaa cgaatactct tcgaaaatct cttcacatca tgtcagcttc
     9181 gtcttgccgt gtccatgggg gacttgactt catcagtttt caaatctgta ctttgagcaa
     9241 gctgagacta gcttcctatt tgattttcat tgaatatcag aaacccattc tccatcaaat
     9301 aattcgactg cgtctaataa tttttgattt ggcaaggtgt cagaaataaa ggttgtgtat
     9361 ttggagaggg gattaatttt aaaaaatcca gtcttgtaaa atttagaact atcaatcagt
     9421 aagatggttt catgggcttt gtcaataata ttcttttttg aaatagcttg gctgagagaa
     9481 gcttcataaa catattggtc atcaatacct cttgctgaac aaaatgctaa atcgatatta
     9541 aaatgatcta ataaagaatt ttccttatca tagttgacca cggaacagga ttgatgtttg
     9601 acctcgccag atgtgataaa gattttggag ctatctttaa cagtttcaga tagggtttgt
     9661 gcagtatgta aaccatttgt aaaaataatc aaattatcaa gttcagaaag atagggacaa
     9721 agttcgtaga cagtcgtact ggaatccaga tagatacaca tgcctgaccg aataaagtct
     9781 ttagcgagac tagcgattag tcttttttgc ctagtacttt ctccttcccg tatttgatga
     9841 gaaagttcaa ttgtgttcat agaggacagg gtcacgtatc cgtgctttct tttgataaga
     9901 ccttgatttt ctaagaaaat taaatcacga cgtaaggtac ttgtgctgga gaaagtgatt
     9961 tctgccagct cttttacggc aattcttttt ttctttttga tgatttcaat caattcaagt
    10021 acacgttcat cttttatcat aagctcctcc taatttatca tttcaactat attatagcac
    10081 aaattggagg aatttga
//
LOCUS       DAYYQN010000066         9699 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYYQN010000066 DAYYQN010000000
VERSION     DAYYQN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.067"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..721
                     /locus_tag="ACHBRQ_001745"
     CDS             <1..721
                     /locus_tag="ACHBRQ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HFM9593363.1"
                     /translation="PDPDGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVD
                     EATYKRNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQ
                     YEKEAKAQGKVITSYGKQIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKEREDKFGKLN
                     KVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLKLKKA
                     IFKAYLDQTGDFRSSIFENKK"
     gene            765..5978
                     /gene="zmpD"
                     /locus_tag="ACHBRQ_001746"
     CDS             765..5978
                     /gene="zmpD"
                     /locus_tag="ACHBRQ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease D"
                     /protein_id="HFM9593364.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNMDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            complement(5983..6168)
                     /locus_tag="ACHBRQ_001747"
     CDS             complement(5983..6168)
                     /locus_tag="ACHBRQ_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593365.1"
                     /translation="MMSKQFLFYDIEIYFILYHAFFILSDDSLLPYLLAYLLPYIEKS
                     HQMMTFQREQKTVPSIF"
     gene            6250..7101
                     /gene="ylqF"
                     /locus_tag="ACHBRQ_001748"
     CDS             6250..7101
                     /gene="ylqF"
                     /locus_tag="ACHBRQ_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9593366.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7088..7867
                     /locus_tag="ACHBRQ_001749"
     CDS             7088..7867
                     /locus_tag="ACHBRQ_001749"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9593367.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7883..9433
                     /locus_tag="ACHBRQ_001750"
     CDS             7883..9433
                     /locus_tag="ACHBRQ_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFM9593368.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNILITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3296 a         1612 c         1992 g         2799 t
ORIGIN      
        1 acctgatcct gatggaaatt ctgtttatgc aacgaatgtt gtacgtagat taaaaccaga
       61 agaacttact aaattaacta catttaacag tttgatagaa catgatatta taacaagaag
      121 aggttatgta gatgaagcaa cttataagcg gaatggctac tatacaatta atctattctc
      181 accaatttat tcagcattga gcagtaaaat aggaacacca ggagatttaa tgggacgacg
      241 tattgcattt gagttattgg cagccaaggg gtacaaagac ggtatggttc catatatttc
      301 taatcagtat gaaaaagaag cgaaagctca aggaaaagtt attacatcat atggtaaaca
      361 aataggttta gttactgatg aaattgttct ttcaaaagta tttaataacc agtataattc
      421 ttggatagat ttcaagaaag atatgtataa agaaagagaa gataaatttg gaaagttaaa
      481 taaagttagt tttatcgatc caaacggttc ttgggctcga caacagaaag tgactattga
      541 taatattaat agacttgaaa aaatgattga agatgcagta aaatttgatg ctgaggatga
      601 agttgccaaa ttatatcctg agacaaatag tagagttctt aaactgaaaa aagcaatctt
      661 taaagcttat cttgaccaaa ccggtgattt tagaagttca atttttgaga ataaaaaata
      721 gtgtttacta ttaggaaata aaaagtgaag gtggaggatt agaattgtca ttattaaaaa
      781 aagataaatt ttccattcgg aaaataaagg gtattgttgg ttcggtattc ttggggagtc
      841 ttttatttgc accgtcagtt gttggtgcat cgacttatca ttacttagat tatagtagtt
      901 tgacacaaac tgaacgtgat caactcaaac aaggtagacc tgatgaatcc aaagagtcat
      961 atgctttgga ttatgaaaaa gacgcactac caaacacagg aagttctcaa tctattatga
     1021 ctgcattggg tttgttagct atcgggagtc ttattgtgat tatcacaaaa gataatagaa
     1081 ataaaaaaat agctacattt ttgattgtag gggcgacagg tttagttaca ctatcaactg
     1141 cttcagcact taatttgaat gctaacatcc atgagtctgg acgtgatggt gtgttgcaaa
     1201 tttctggtta cagatatgtt ggttacttgg agcttgatga taaaactgtt tcgtcggttt
     1261 ctccagcatc aactgtttcg ccagttgagc aacccaaagt tgtaacagag aaaggcgaac
     1321 cggaagttca tgagaagcct gactatacgc aaccaatagg tgcaaacctt gttgagcctg
     1381 aagttcatga gaaactagca tacactgaac cagtagggac aacaggagta gatgaaaatg
     1441 gaaacttgat tgaaccacct gttaacgaca tcccagaata tactgagcca gtaggtacaa
     1501 caggagtgga tgaaaatgga aacttgattg agccacctgt tagcgacatt ccagagtata
     1561 ctgaaccaat atctacagtt tcagaagttg catcagaaag agaagagttg ccttctttac
     1621 ataccgatat tcggacagag actattccta aaaccactat agaagaatct gatcctagca
     1681 aatttatagg cgatgattct gttagacaag ttggtgagga tggcgaacgt caaattgtta
     1741 ctagctatga agagttacat ggcaaaaaaa tcagtgaccc agttgaaaca gtaactattt
     1801 tgaaagaaat gaagcctaaa attcttgtaa aaggtaccaa agaaaaacct aaagaaaaaa
     1861 cggctcctgt tttgacctta gatagaacaa atacaaatgt attaaaccga tccgcaactc
     1921 tttcttatca tttagttaat acagatggag tcacaatcaa taaaattact gcgacaatta
     1981 aagatggaaa tgaaattgtc aaaacagtag atttgacttc agagcaatta gataagcagg
     2041 tagaagattt aaaattctac aaggactata agatagagac cactatgacc tatgaccgtg
     2101 gcaagggaga agaaactgca acattagaag agaaaccact tcgcttggat ttgaaaaaag
     2161 ttgaaattaa aaatatcgca tctactaatt tagtgaaggt aaatgacgat ggtactgaaa
     2221 cacctagtga ttttatgact gaaaaacctt cagatgaaga tgtgaagaaa atgtacttaa
     2281 aaattactag tcgtgataat aaagtaacac gtttagcagt tgataaaatt gaagaagtga
     2341 ctgaagaagg taagaaactt tataaaatca ctgccgaagc tcaagatttg attcaacata
     2401 cagacccaac gaaggttcgt aacaagtatg ttcattatat tgaaaaacca gttccaaaag
     2461 ttgatgacgt ttattataac tttaaagagt tagtagatgc catgaatgct gataaaaatg
     2521 gtacatttaa aatcggagca gatttgaatg caacgaatgt accaacacct aacaaacaat
     2581 atgttccagg tacgtttaag ggacatctat caagtgttga tggtaaacaa tatacaattc
     2641 ataacattgc ccgtccctta tttgaccgtg ttgaaaatgg ttctgtgaaa aatattaatt
     2701 taggtaatat ggatatcaat atgccttggg ctgacggaat agcacctgtt gccaatatgg
     2761 tgaaaaatgc aactgttgaa gatgttaagg taactggtaa tgtagttgct aataacaata
     2821 ttgcgggtat cgtcaataaa atcgatagtg gtggtcagtt gacaaatgta gccttcatcg
     2881 gtaatcttac aggtgttggt gataaaggtc aatatatggc tggcattgcc ggtgagattt
     2941 ggagaggtaa tcttgcaaaa gcatatgtcg aggctgatat tgttgctaac agagcccgta
     3001 taggtggttt agttgctaaa acagataatg gtaacgactc tatgggaatt ggtaaatacg
     3061 gttcaattcg taaatcagtc accaaaggaa ccattaagac taaagttttg tttgaaacag
     3121 gtggatttat caatagcaat cttccttttg gtaaacttga agataatatt tcaatgatga
     3181 gagttgaaaa tggtgaagag ttctttggtt cttccgattt ggactacgat ggtggttatt
     3241 tcacaaatgg ctggttagag cgtaactttg ttgtcaaagg tgtcagttca ggtaagcatt
     3301 catacaaacg ttctcgtgac aaaattaaag aaattagtca agatgaagct aataagagaa
     3361 ttgcaaattt tggacttact gctgataaat acgaaatcaa cgaacctgtg gtcaatcgct
     3421 taaatcgttt aaccagaaga gaagatgaat ataagtcaac tcaagactac aaatccgaac
     3481 gtgatttagc ttatcgtaat attgagaaac ttcaaccatt ttataataaa gaatggattg
     3541 tcaaccaagg taacaaactt gcagaagatt caaacttagc taagaaagaa gtcctttctg
     3601 ttacggggat gaaggatggt caattcgtga ccgatttatc agatattgat aagattatgg
     3661 ttcactatgc tgatggtact aaggaagaaa tggatgttac taagaatact gattctaaag
     3721 tccaacaagt gcgtgaatat agtgtatctg gtcttggtga tgttgtttat acaccgaata
     3781 tggttgttaa aaatcgtgat aaattgattg ctgatgttaa atctcaatta tcaagtgtgg
     3841 aattgatttc acaagaagta agagatttaa tgagtagacg tgataaacct gcagagaata
     3901 cggatgaacg taagaatggt tatattaaag atctatactt agaagaaagc tttgccgaag
     3961 tgaagcaaaa tctagataag ttagtcaaat ccttggtcga aaatgaagac catcaattga
     4021 atggtgatga agctgcgata aaatcacttc tcaagaaagt ggaaaccaat aaagctaaaa
     4081 tcatgatggc tctgacttat ctcaatcgtt actatgatat caaatacggt gacataagta
     4141 taaaaaacat catgatgttc aaacctgatt tttatggtaa aacaccaagt gttattgaca
     4201 gactcatcaa catcggttca agtgaaaaga acttgaaagg ggatcggact caggatgctt
     4261 accgtgaaat catcgcaggt aatactggta aaagtaattt gcgtaacttc ttagaataca
     4321 acatgcgtct gtttacagag gataaagaca tcaatgattg gtttatccac tcagctaaga
     4381 atgtttatgt ttcagaacct aagacaacaa atacagagtt gaaagataaa cgtcatcgtg
     4441 tgttcgatgg tttagataac ggtgttcatg gtcgtatgat tttaccactt ctaacgctta
     4501 aagatgccca tatgttctta atttcaacat ataacacgat ggcgtacagt tcatttgaga
     4561 aatatggaaa acatacagaa gaagctcgta atgagttcaa aactaagatt gatgaagttg
     4621 cacatgctca acaaacttat ttagatttct ggtccagatt agctctgcct aatgttcgtg
     4681 atagacttct taagagtcaa aatatggtac cgacaccagt ttgggataac cagacctaca
     4741 atggttctcc tgtaggtcgt cgaggatttg acagtaaagg taaccctata gctccaattc
     4801 gtgaattata tggtccaaca tggcgacacc atgatagaga ctggcgtatg ggcgctatgg
     4861 catcaatatt ccctaatcca aataatgatg acaaggtctt gtttatggtg acggatatga
     4921 ttagcccatt tggtatttca gcctttactc acgaaacaac acacgtaaac gatagaatgc
     4981 tttacttcgg tggtcataag catcgtcaag gtacagacgt tgaagcctat gctcaaggta
     5041 tgttgcaaac acctgacagc tcaacgacta atggtgaata cggcgcactt ggaattaaca
     5101 tggcttacca tcgtccaaat gatggtaacc aatggtacaa cccagatcct gacaaattaa
     5161 agacacgtga tgacattgac cgctacatga gaaactacaa tgaagctatg atgttgcttg
     5221 atcatgtaga agctgatgct gtacttccta aaattaaagg agacaatagt aagtggttca
     5281 agaagattga caaagaaatg cgctctaaga ttcagtataa tgatctttta ggtccaaacc
     5341 aatgggatag tatccgtgat ttgaaagatg aagaaaaggt aatgacttta tctagcgtca
     5401 atgatttagt ggataataac ttcatgacaa aacatggaaa tccaggtaat ggacgttata
     5461 gaccagaaga ttttacacca aactcagctt acgtaaatgt caatatgatg gcaggaattt
     5521 acggtgggaa tacgagtcaa ggtgctcctg gttcattatc atttaagcac aatgctttcc
     5581 gtatgtgggg ctattatggt tatgaaaatg gctttattag ttatgtatca aataaataca
     5641 aagcagaagc agataaaaac aaccacggtt tactaagcga taagctaata attaataaag
     5701 tatcgaaagg aaactttaat actcttgaag agtggaagag acattggtac ggggaagttc
     5761 ttgctaaagc taagaaaggt tttgaggcta tcgatattga tggcgtccat atcagtaact
     5821 atgatgagtt aaggccttta tttgataaag cagttgaaga agatttgaag aaaccagatg
     5881 acttctctca cactgttgct cttaaatcta aggtcttcaa agcccttctt aaaaacacag
     5941 acggtttctt taataaactg tttaaagaag acatttaaat gtttagaata tagaaggaac
     6001 agttttttgc tccctctgaa aagtcatcat ttgatggctt ttttctatat agggtaaaag
     6061 ataggctaaa agatagggta aaaggctatc atcggacaaa ataaagaagg catgatataa
     6121 tataaagtag atttctatgt cataaaacaa gaactgtttg gacatcattc atttgaaaac
     6181 tctctatgtt caaactatag taaaataaaa taggggatct aaatccttgc tacgaaagga
     6241 aaaaactcaa tggctactat tcaatggttt cctggtcaca tgtctaaagc gcgtcgacag
     6301 gtgcaggaaa atttaaaatt tgttgatttt gtgacgattt tagtagatgc acgcttgcct
     6361 ctatctagtc aaaatcctat gttgaccaag attgttggtg ataaaccaaa actcttgatt
     6421 ttaaacaagg ccgacttggc tgatccagca atgaccaagg aatggcgtca gtattttgaa
     6481 tcacaaggaa tccagacgct agctatcaac tccaaagagc aagtgactgt aaaagttgta
     6541 acagatgcgg ccaagaagct catggctgat aagattgctc gccagaaaga acgtgggatt
     6601 cagattgaaa ccttgcgtac catgattatt gggattccaa acgctggtaa atcaactctg
     6661 atgaaccgtt tggctggtaa aaagattgct gttgttggaa acaagccagg ggtcacaaaa
     6721 ggtcaacaat ggcttaaaac caataaagat ctggaaatct tggatacacc ggggattctc
     6781 tggcctaagt ttgaggatga aactgttgca cttaagttgg cattgactgg agctatcaag
     6841 gatcagttgc ttcctatgga tgaggttacc atttttggta tcaattattt caaagaacat
     6901 tatccagaaa agctggctga acgcttcaaa caaatgaaaa ttgaagaaga agcgcctgtg
     6961 attattatgg atatgacccg cgccctcggt ttccgtgatg actatgaccg tttttacagt
     7021 ctcttcgtga aggaagtccg tgatggcaaa ctcggtaact ataccttaga tacattggaa
     7081 gacctcgatg gcaacgatta aagaaatcaa agaactcctt gtgacagtca aggagttaga
     7141 aagccctatt tttttagagc ttgaaaagga taatcgctca ggagttcaaa aggaaatcag
     7201 caagcgtaaa agagccattc aagctgaatt agatgaaaat ttgcgcttgg aatccatgct
     7261 ttcttatgaa aaagaacttt ataagcaagg attgacctta attgcaggta ttgatgaggt
     7321 tggtcgtggt cctcttgctg gtcctgtagt cgctgcggcc gttattttac ctaaaaattg
     7381 taagattaaa ggtctcaacg acagcaagaa aattcctaaa aagaaacatc tggagatttt
     7441 ccaagccgtt caagaccaag ccttgtcgat tggaattggt atcatagata atcaggtcat
     7501 cgaccaagtc aacatctatg aagcaaccaa actagccatg caagaagcaa tctcccagct
     7561 cagccctcaa ccagagcacc ttttgattga tgccatgaaa ctggacttgc ccatttcaca
     7621 aacctccatt atcaaaggag atgccaactc cctctctatc gcagcagcat ctatagtagc
     7681 caaggtaaca cgtgatgaat tgatgaaaga atacgatcag cagttctctg gctatgattt
     7741 cgctactaat gcaggatatg gcacagctaa acatctggaa ggcctcacaa aactaggagt
     7801 taccccaatt caccgaacca gctttgaacc cgttaaatca ctggttttag gtaaaaaaga
     7861 aagttaattg aaaggaaata ccatggagga acagtcggaa atagtccgtt ctaagaaaga
     7921 attcgccttt gcatccagca ctatactatc ccaagttggt cgaggaatca ttgtcggcct
     7981 catcgttgga attatcgtcg gatcctttcg tttcttaatt gaaaagggct tccacctgat
     8041 acaaggagtt tatcaagatc aagggtactt agtgcgcaat ctttttgtac tggttttgtt
     8101 ttatatactc atctgttggc tcagtgccaa actaacacgg tcagaaaaag atattaaagg
     8161 ctcaggaatt cctcaagtcg aagccgaact gaaaggcctc atgtccctca actggtgggg
     8221 cattctttgg aaaaaatatg tgctaggtat tcttgctatt gccagtggac tcatgctggg
     8281 tcgagaggga cccagcattc aacttggagc agttggtggt aaaggaattg ccaagtggct
     8341 caaatccagt ccagtagagg aacgttcctt gattgccagt ggagctgcag caggtttagc
     8401 cgcagccttt aatgctccta ttgcagcact tctctttgtt gtagaagaag tctatcacca
     8461 tttttcgcgc tttttctggg tctcaactct agcagccagc atcgtagcaa actttgtgtc
     8521 tctactcatg ttcggtttga caccagtatt ggatatgcca gataacattc ctcccatgac
     8581 cctagaccag tattggatat atctcgtcat gggaattttc cttggatttt caggttttct
     8641 ctatgagaaa gctgtattaa acgttggaag agtttatgac ttgattggtc aaaaaatcca
     8701 tttggatagg gcttattatc ccatcttggc ttttatcctt atcataccag tcggaatctt
     8761 cttacctcaa atcattggtg gcggaaatca gcttgtcctt tctttaactg aacaaaattt
     8821 tagtttccaa gttttattag cttacttttt aatccgcttt atttggagta tgattagcta
     8881 tggaagtgga ctgccaggag gaattttcct ccccatttta gctcttggtt ctttgcttgg
     8941 tgccttagtt ggtgttatct gtgtcaatct tggacttgtc agtcaagagc aattccctat
     9001 atttgtcatt ctaggaatga gtggctattt tggagccata tcaaaagctc ccttaaccgc
     9061 tatgatcctc gtaactgaga tggtaggaga tattcgcaac cttatgccac ttggtcttgt
     9121 cactcttgtt tcttatatta tcatggattt gctcaaaggt acgccagtct atgaagccat
     9181 gctggaaaaa atgcttccag aagaagtatc tagcgaagga gaagttacac ttatcgaaat
     9241 accagtttct gataaaattg ctgggaaaca agttcatgaa ctcaacttac cacacaacat
     9301 cctcatcaca actcaagtcc ataatggcaa gagccaaaca gttaacggct caaccagaat
     9361 gtatctgggt gatatgattc acctggttat tccaaaaagt gaaattggaa aagtcaaaga
     9421 tttgttgttg tagtatgatc atttacataa tttatgttat gtaaatgatc ggtttgattt
     9481 atttagaaaa ccgattctca gaaatgagat cggttatttt ttactgatga ggaattttac
     9541 atataaataa ttgaacttta ttaaaaataa gactataatt aagttagaaa tgataaagca
     9601 taaagctaga aaggagttta ctgtatcaaa tctgtacagt aagattaaaa tcatgaaaaa
     9661 gaaaacaata gcaattatat agtgaaatga aatagaaat
//
LOCUS       DAYYQN010000067         9590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYYQN010000067 DAYYQN010000000
VERSION     DAYYQN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.068"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            90..389
                     /locus_tag="ACHBRQ_001751"
     CDS             90..389
                     /locus_tag="ACHBRQ_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593369.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLKVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            379..867
                     /locus_tag="ACHBRQ_001752"
     CDS             379..867
                     /locus_tag="ACHBRQ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593370.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYAIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     AKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            867..2744
                     /locus_tag="ACHBRQ_001753"
     CDS             867..2744
                     /locus_tag="ACHBRQ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9593371.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQESDERWWNYQINPLNQRQENYLDDLRIRDLTFESSL
                     K"
     gene            2765..3007
                     /locus_tag="ACHBRQ_001754"
     CDS             2765..3007
                     /locus_tag="ACHBRQ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593372.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            3024..3878
                     /locus_tag="ACHBRQ_001755"
     CDS             3024..3878
                     /locus_tag="ACHBRQ_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HFM9593373.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTLSGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            3932..4291
                     /locus_tag="ACHBRQ_001756"
     CDS             3932..4291
                     /locus_tag="ACHBRQ_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM9593374.1"
                     /translation="MNTCVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            4242..6599
                     /locus_tag="ACHBRQ_001757"
     CDS             4242..6599
                     /locus_tag="ACHBRQ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM9593375.1"
                     /translation="MKRKSNTLKKQKTSMTNKKEEVKGKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDQTNFQLTIFNKR
                     LNLEKFRQSVLYEEKEDGYDTYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFESLAGEDRVNLLADMLRGEHHLPFSYRDLTRSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGIFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNVAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRAFGGEMIDIAP
                     DSQTYLNVLDLSDENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQPEEEAQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            6611..9424
                     /locus_tag="ACHBRQ_001758"
     CDS             6611..9424
                     /locus_tag="ACHBRQ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9593376.1"
                     /translation="MKDKRKIIRARKAFRRSLKDEKKFLKKGKKEVRKQKKDSAVLDE
                     KAWKKEIKEKLEEMREASKERVKQANEDYNHILQNSPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFREAKVEAKEERKESRKERKTNQKFLYGQETKAKSNFFFQGKSLEELKAKKE
                     VKDAKENLKSTKQAYKSKKVSRKAKTFLYVLGREGGELASENEDLEGYRTLQETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLAKDNPSSFKNR
                     YRKLKKQGLTSVRNIYQKLKAAFSFFTFAAGNPATWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELHKPV
                     HLFSSETYKDYLSTLWHDLNDGEDLKSMQDLYETPKYKLSKDDQEEMKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQAIVAPMDGVVSL
                     DGDNVILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKFKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYEFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATASSWDDETWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYAHSKNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VSTASSLGGETGSIPRAGAIVSFVGGTHGTPAIYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISQADSAISFAYTVK"
BASE COUNT         3189 a         1595 c         2005 g         2801 t
ORIGIN      
        1 acaagacgtg tgtgagaatt ttctaacaga cacggagttt atcgttacca gacttaaacg
       61 atgcgacaaa ctagataaag gagttaatca tgaaggtagt aaacttgtat gatttgaaac
      121 aaatgggaaa taaaggtggt tgtaccatcc aattgattca tcactttcct tttggtatgg
      181 gcttaggaca tctcaaaaaa gactacattg aatttaaacg tgttggtatc gttgatggga
      241 aggcagtaga agtcactctt cgagaacctt attcgagaga tctactgaag gttgtcaagt
      301 cgattaagca acgtcagaaa ttgatagctt atcgttataa agaaggaaag ctgctatttg
      361 tgaagaagga ggcatcagat gtcctctgaa caacaggaac gaatggcagt tcaatatgca
      421 gagcgtagtc ttttatttac tgtaaaaagt cttttaaaga ttctagaatg gtctagacgt
      481 caggctttag cacaggattc cgcctataag ataggagtgc agaaattaga agaattgcta
      541 caatctccct atgcgattga tacgattaat ctgaaaaaag attttttaga caaaccaatt
      601 gatatagaga aatttaaagc ttttttagaa aaagaagaga ttcctttagc catcgcttgg
      661 caaggggatt ctctacattt ctacgcgaaa gaccgttcga ttttagacaa tcatttagac
      721 catcttttag aaaaaatggt taatgatccg gagaaattag ctgattttac catggataag
      781 tcattagatg atgctattga tgaagctaaa tcccaaatta cctttagaca agaaggagcc
      841 gtcaaacaga aagagatggt gagatgatgt acagtggaaa aaaattccta ctattctcac
      901 tgttaggtat cttactaggc tatctttttc atcgtttgac gcttttgtat gattcctata
      961 ctggaaatac cttagataaa tggactcatc ttctgatgga aggtcaagat gaagttcttc
     1021 agtcgccatg gaatgtttct tttactggaa aatcaagtgc tttttttcta ctaggttttg
     1081 tgatgatgtt actggtttat ctctatctag agactggtaa aaaacaatac cgagaagggg
     1141 tagaatacgg gagcgcccgt tttggaactc taaaagaaaa gaagctcttt tacggtaagg
     1201 aattttctca tgatacgatc ttagcacaag atgttcgttt gacattatta gataaaaaac
     1261 caccccaata tgatagaaat aagaatattg cggtgattgg aggttcagga agtgggaaga
     1321 catttcgctt tgtgaaaccc aatctgattc agatgaatag ttctaatatt gtagtggatc
     1381 ctaaagatca cttggccgag aaaacaggca aactcttttt agaacatggc taccaagtaa
     1441 aggtgttaga tttagtcaat atgaagaact cagatggctt taatcctttt cgctatatag
     1501 aaacagaaaa cgatttgaat cgcatgctga ctgtttattt taataacacc aaaggctctg
     1561 gctcccgaag tgatccattt tgggatgaag cttctatgac tttggttcga gctttagcct
     1621 cttacttagt cgatttctat aatccaccta aaacaagaga acagctcata gaagaaagtc
     1681 gtttaagtca aaaggaatat caaaacttgt tgaaacgtca aaaaaaagaa gtggaagagc
     1741 gaaaaaaacg aggtcgttat ccaagttttg ctgaaatctc aaaactcatt aaacacttat
     1801 ccaagggtga aaaccaggaa aaaagtgtct tagaaattct atttgaaaat tatgctaaaa
     1861 agtatgggac tgaaaatttt accatgcgaa attgggcaga ttttcaaaat tataaggata
     1921 agactctgga ttctgttata gctgtaacca ctgctaaatt tgccctcttc aatattcaaa
     1981 gtgtcatgga cttgaccaaa agagataccc ttgatatgaa gacatggggc aaggaaaaat
     2041 caatggttta cttagttatc ccagataacg atagtacctt tcgctttctt tcagccctct
     2101 ttttttcaac cgtatttcaa accctaacaa gacaagcaga tattgatttt aagggtcaat
     2161 tgcctcttca tgtgagagtt tacttagatg agttcgcaaa tatcggagaa attccagatt
     2221 ttgctgaaca aacctcaaca gtccgttctc gaaatatgag tctcgttcct attcttcaaa
     2281 atattgccca acttcaaggg ctctataaag aaaaagaagc ttggaaaacc attttgggga
     2341 actgtgatag cttagtctac ttaggtggta atgatgaaga tacctttaaa tttatgagtg
     2401 gcttactcgg taaacaaacc attgatgttc gaaatactag tcgttccttt ggccaaacag
     2461 gttcaggatc cctttctcat caaaagattg ctcgtgactt aatgacaccc gatgaggtcg
     2521 gaaatatgaa acggcatgaa tgcttggttc gaattgccaa tatgcctgtc tttaaaagca
     2581 aaaaatacaa ttccactaag catccaaact ggaagtacct agctaatcaa gaaagcgatg
     2641 aacggtggtg gaactatcaa atcaatcctt tgaatcaaag acaagaaaat tatcttgacg
     2701 accttagaat tcgtgattta acttttgaat ctagtttaaa ataaaaatag aaaagaggaa
     2761 atagatgatt acgcatttta aaggttttgt ttatggagta gacgcaagtg ctatgtttgc
     2821 acaagctatg tctttgttac agaagggatt gattgcagtt ggtgcctttc tcgttgttgt
     2881 ggggattgtc aaccttgcaa ccaacattaa agatggtgga ccaggtgttc ggaatgccat
     2941 tcttgaaatt gtcggtggtg ttatggtagg ggctgctgga gcctttgtaa cccagatttc
     3001 aatttaggag gataaacaat gacatgaatc ttagtttagt ctcacccttt gtttaccttg
     3061 catctgaaaa aatatcagct gaaaatttat ttgaaggatt taatgtggat ttacaatcta
     3121 cggtagatct gattaaatcc ctatctagct acaatccaac agtttggact tatatgtcta
     3181 gtattactaa aagtgtcatg cagcctcttg gagttgcgat tttatcagtt gttctcatct
     3241 tagaattttc gaagatggca aagaagattg ctaactctgg tggagcgatg acctttgaag
     3301 cattagcgcc gatgttgatt agttatatta tggtcgcagt ggtcattacc aatactaccg
     3361 ttatagtaga agctatcatc gggattgcga gtcacgccat cgaacaagtg gcctcgattg
     3421 tggctcacgg tggggcgaag tatgatacac tctctggatt aaaaggttca ggatttattg
     3481 gccggatgat tgtgggcttt ttcgctctcc ttatctggct tgttcggata gtaagtgcag
     3541 ccatggttaa tcttttggta tctattcgat ttattcaact ctaccttatg atcccatttg
     3601 cccctcttac gattccaaca tttttaagtg atgaatggaa gtctattggt attggctatt
     3661 taaaaaatat tatggtctat gcggtacaag gggttctcat ttttctgatt gtttctcttg
     3721 ttcctttgtt tgaatctgct gggaaaatag ctgtttcaaa tggtgcagga gtcttgcaat
     3781 cacttgcgat tatgtttggt agtttagtac aagctatctt actgattatt gccctcgttg
     3841 gttctcaacg tacggctcgc tcaatcttag gtatgtaatt agataaaggc taggaagtga
     3901 ttgcttctta gcctttttag aaaggaaagt catgaataca tgtgtcttta aagacatctc
     3961 aaaataccaa cacagggctt ggttaggttt caccacaaga caaatcatct ttgttttacc
     4021 agcctttatt gtcacaatta ttgttttggg cttgaatctc tttttctggc aatttggaga
     4081 ttggtttgtt tacggttttg tgtttgcttt taccatcccc ctaatgcttt ttggagtcta
     4141 taaacccaat gatttatatt ttgaacatta tttgaaatac cgtcttcatt ttgaattaac
     4201 ggttccccta cgcacaatta caggaaagaa aggacctgaa catgaaaaga aaatcaaata
     4261 cattaaagaa acaaaaaact tcaatgacta ataaaaagga agaagttaaa gggaaaaaag
     4321 aggaagtgtt accctcaacg gctaatactc tttcctatca agccctgtat caaaatggtc
     4381 tgatgcaggt aaaagaagat tatttctcac aaagctattt acttggtgat gtcaattacc
     4441 agaccgttgg tttagaagat aagggcgcaa tcattgagaa gtattctgat ttgattaact
     4501 cactagatga ccaaaccaac ttccaattga ctatctttaa taaaagattg aatttagaaa
     4561 agtttagaca aagtgttttg tatgaggaaa aagaagatgg atatgatacc tatcgtaaag
     4621 aattgaatcg gatgatgaat cagaatttag atagtggtga aaataacttt tcggctgtaa
     4681 aactgattag ctttggaaga aaggattcta atcccaaaca agcctatcgt tccttgtctc
     4741 aaataggaga atatttcaag agtggtttct cagaaattga tgctcgattt gaatccttgg
     4801 ctggagaaga tcgtgtgaac ttgttggccg acatgcttag aggagaacac catcttcctt
     4861 tttcttaccg tgatttaacg agatctggtc agacaactcg tcacttcata gcccctaatc
     4921 tcttggattt taaaaacaag aattacctac aaatcaatga ccgcttatta cagattgtct
     4981 atgtcagaga ctacggcatg gaattagggg atcagtttat tcgagacctt atgcaaggag
     5041 atttggaatt gattgtaagc cttcatgctc aaagttcgac caaggcagat gccatgaaga
     5101 aactacgaac aaagaagacc ttgatggaat cccaaaagat tggggaacaa caaaaactag
     5161 ctcgtacagg tatctatttg gaaaaagtag gtcatgtatt agaaagtaat atcgatgaag
     5221 ctgaagaact tttaaaaacc atgaccgaga caggtgataa actgtttcaa acggtcttct
     5281 tgattgggat ctttggtcag gatgaagaag aactcaaaca agctctagat acgattcaac
     5341 aagtggccgg ctcaaatgac ctaatgattg ataaacttcc atatatgcaa gaagcagcct
     5401 ttaatagttt gctgccattt ggttgtgatt ttttagaggg agtatcacgg agtttattaa
     5461 catctaatgt agcagtgaac tcaccttgga cttcagtaga cttacaagac cgcagtggga
     5521 aatattacgg tatcaatcaa atctcaagca atattattac tattgatcga agcttattaa
     5581 atacaccgtc tggtctgatt ttagggacat ctggagctgg gaaagggatg gcaactaagc
     5641 atgagattat cacgaccaaa atcaaggaat ctggtgaaaa tactgaaatt atcatcgtgg
     5701 atccagaagc agaatacagt gtcattggac gagcttttgg gggagaaatg attgatattg
     5761 cgccagattc ccaaacctat ctcaatgtac ttgacttgtc tgatgaaaat atggatgagg
     5821 atcctgttaa ggtaaaatca gaatttcttt tatcctttat cggcaagtta ttggatagga
     5881 aaatggatgg aagagaaaaa tcgattatcg accgagtcac cagactcacc tatcagtcat
     5941 ttaaagagcc ttctttggaa gaatgggtct ttgtcttgag tcaacaacca gaagaagaag
     6001 cgcagaattt ggcacttgat atggaactgt atgtcgaagg ttctcttgat attttttctc
     6061 ataagaccaa tattcagaca ggatctaact tcttaatcta taacgttaaa aagttaggag
     6121 atgagctgaa acaaatcgct cttatggtag tgtttgatca gatatggaat cgtgttgttc
     6181 ggaaccaaaa attagggaag aagacctgga tttattttga tgaaatgcag cttctcttat
     6241 tagataaata tgccagtgat ttcttcttta aattgtggag tcgtgtcaga aaatatggag
     6301 ctagtccgac tgggataact caaaacgtcg aaaccttatt gttagatcca aatggtagac
     6361 ggattattgc aaatagtgaa tttatgattc tcctcaagca agcaaaaaat gaccgagaag
     6421 aactggttca actcttaggc ttgtcaaaag aactcgaaaa ataccttgtc aatccagaaa
     6481 aaggggcagg actgataaaa gctggttcag ttgtcgtacc ctttaaaaat aagattcctc
     6541 aaggtactca attgtttgat atcatgagta cggatcctga taaaatggct tctaattaag
     6601 gggaagtaga atgaaggata aaagaaaaat catacgtgcc cgaaaggcat ttagaagaag
     6661 cctaaaagat gagaaaaaat tcttgaaaaa aggaaagaag gaggtaagga aacagaaaaa
     6721 agattccgct gtactggatg aaaaagcatg gaaaaaagag ataaaagaaa agctagagga
     6781 gatgagagag gcttcaaagg agagagtaaa acaagcaaat gaagactaca atcatattct
     6841 tcaaaatagt cctccatctc ttttgaatcg caaagaatta agagacagac gattacctca
     6901 tgctaggaaa cgattgaaaa tagctaagaa gcaatttaga gaagccaagg tagaagcaaa
     6961 agaagaaaga aaagagagtc gtaaagaaag aaaaaccaat caaaaatttc tttatggtca
     7021 ggaaacaaaa gcaaaatcca attttttctt tcaagggaag agtttagaag aattaaaagc
     7081 taagaaagaa gtcaaggacg ccaaagaaaa tttaaaatct actaaacaag cctataaatc
     7141 caaaaaggtt agtaggaaag ccaaaacttt cctttatgtc cttggacgtg aaggtggaga
     7201 gttagcttca gaaaatgaag atttagaagg ctatcgcaca cttcaagaga ccattagaaa
     7261 agggaaacgc tacagtcggc tttcttataa tcttggaaaa gctagtgtca aaacaggaca
     7321 agcaacaggt cgttttacca agaaaagact gaccaacaca aaagagcgat accatcattt
     7381 taaggatgga aaaggatgga aactagcgaa agataatcca agttccttta aaaatcggta
     7441 tcgaaaatta aagaaacaag gccttacaag tgtacgaaat atctatcaaa aactaaaagc
     7501 agccttttcc ttctttacat ttgcggctgg gaatcctgca acctggatag ttggaggaat
     7561 agtctttctt cttttactta tgatgagctt ctttttagga ttttcatctg ctagtttaat
     7621 tcaacaagat gaatttgaat taacaaaagc ttatacccac ctaacttggg aagacgcaga
     7681 acatacccga accaatgaca aaggaattac ctattacaca aaagttgatg atgtgatggg
     7741 gtatatgaac tttaaattcc atgactatga gttacacaaa ccagttcact tatttagttc
     7801 agaaacttac aaggattatc tatctacttt gtggcatgat ttaaacgatg gggaagattt
     7861 gaaatccatg caagaccttt atgaaactcc taagtataaa ctatcgaaag acgatcaaga
     7921 ggaaatgaag gaactaaaag aagaaggtgt gtatgcttcc atgcaggaat tggacaatcc
     7981 atttgaaggg aaaagtaacg aagatagttt aaccatgact tatcgttatg gatactatga
     8041 tttagacgga aaacctactc ttcaggagta cattctacta gaagctaaag ctcaccaagc
     8101 aattgtcgca ccaatggatg gagttgtatc tctagatggc gacaatgtta ttctcactaa
     8161 cggaaaagga gagaatgaga gtcgattaac cttgtattct attcataatg gccgtgcgat
     8221 tgaggggaca agagtcctaa cgggtgatat tattggtgaa acaccagacg atacaggttt
     8281 gaaagtttcc tatcaaaagt ttaagaacaa gaaagaaaaa ttggtgtacg tcaatccgca
     8341 attttatttt ccaaaagtca ttcaacttca gacaactatc ttacctgcca ttggtcagtt
     8401 tggtggggat gagtttgaac gagcgaaaca tatttatgag tttttgaaat ctcaaggggc
     8461 aagtccccaa gccattgcag ctattttagg aaattggtcg gtagagtctt ctatcaatcc
     8521 taaacgagct gagggagatt atttatctcc tccagttggc gctaccgctt cctcatggga
     8581 tgatgaaacc tggttagcga ttggaggtcc agccatttat agtggtgctt atcctaatat
     8641 ccttcataga ggtttggggt taggtcaatg gacggatact gcagatggtt caacacgtca
     8701 tacagcttta ttgaattatg cacatagcaa aaataagaag tggtatgatt tagacctcca
     8761 acttgatttt atgcttcatg gggatagtcc ttactatcaa agttggttaa aagatttctt
     8821 taaaaatacg ggcagtgcag ccaatctcgc ccaactcttc ctcacctatt gggagggaaa
     8881 ttctggtgac aaactactgg aaagacaaac cagagcaacg gaatggtatt accaaattga
     8941 aaaaggcttt agccaaacaa acggaggaca agcaaagagt gacccacaat cccttgaagg
     9001 ggttcgtggg gacttgtatg atcattctgt tcctggtggt ggagatggta tggcctatgc
     9061 ctatggacaa tgtacatggg gagttgcggc tcgtatgaac cagttaggct taaaattaaa
     9121 aggaagtaat ggagaaaaga tttcaatcat taataccatg ggaaatggtc aggattgggt
     9181 ttcgacagct tcaagtcttg gaggggaaac gggctctata ccaagagcag gtgctattgt
     9241 ttcttttgtg ggaggtacac atggcacacc agccatctac ggtcatgtgg cttttgtaga
     9301 gaaagtctat gatgatggtt ctttccttgt gtctgaaacc aactatggtg gcaaccctaa
     9361 ctataccttt agaaaaatct cgcaagcaga tagtgccatc agttttgctt atacggtgaa
     9421 ataaaagaat catgtcatca gtttaggtga catgattctt attttttata gagtgacata
     9481 tttaatgcca taggctgata agttacttga tgatcgtctt taatcaattg actgtaagca
     9541 acaaatccga actgttcata aaattgaatg actttaggat gattgatact
//
LOCUS       DAYYQN010000068         9343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYYQN010000068 DAYYQN010000000
VERSION     DAYYQN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.069"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            149..291
                     /locus_tag="ACHBRQ_001759"
                     /pseudo
     CDS             149..291
                     /locus_tag="ACHBRQ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            490..900
                     /locus_tag="ACHBRQ_001760"
     CDS             490..900
                     /locus_tag="ACHBRQ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HFM9593377.1"
                     /translation="MEAIYQRDSDQDGLTDAQELALGTNPLSADSDGDGRSDLVEVEE
                     GTNPLEKDLQDIDQTSITEPSSVFMEMKQKISDMMESHYKEFIQALISIETGIENQQD
                     LEDLYTYYMRTDSVSLLSSDLETSPQEVEMEIEL"
     gene            948..7172
                     /locus_tag="ACHBRQ_001761"
     CDS             948..7172
                     /locus_tag="ACHBRQ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM9593378.1"
                     /translation="MEVMQLLAMFRGTIPKDREKMDLFLRYQAQHFDEKWQDLVENFL
                     TEEGKIEEIPHVYSFDQDIVSFLEASSENNDQDLESYTRSFGQAGLGKLSQLSNFEKN
                     LVLEVAIYNLSTRFYIQSEKEKLTPLSELVCHQNQDVNLVNVYRVANNLSDRISRDIE
                     EFLLMVDSKEVKKEVLEIHFEGKEGDVLAYLGSELMATLDSVTELVHHEENYQQLPLT
                     QKLKIITHFDDVKAKIEKSKRVAEVVLPLDNIEQVEENVEVHSLSKVDKIVEEALREY
                     PIGSQVSYRGQVFQLVSIENAQLNDLVRLELFNDSNQLFEENPILYLNSLEEIEQVLS
                     LVELEKEDSEIEIESPSESQEIDLFSYLEEENEKDKDKETKPLISGIEETEVPVQDFV
                     FPDDLEDFYPKTNREKIETNIAAIELVKRLEKEGRQANPEEQELLAKYVGWGGLANEF
                     FDELNPKYETERLTLRSLVSKSEYSTMKQSSLTAYYTDPMIIRQIWQKLLDDGFGGGR
                     ILDPSMGTGNFFAAMPRSIRDKSELYGVELDSVTGAIAKQLHPNVHIEVRGFEEVPYQ
                     NNSFDLVLTNVPFGNFRIADKNYDKPYMIHDYFVKHSLDLVRDGGQVSIISSIGTMDK
                     RTDNVLQEIKSNTHFLGGVRLPDTAFKKIAGTRVTTDLLFFQKDQAKNLNEEELVFSG
                     SIPFEEDKRVWINPYFDGKYNTQVLGKYEVRNFNGGTLNVKGGSETLATDIMKALENV
                     EEPKPIDNSLKAPVFIQEEVDNSIPSRIREDLALYSFGYEGNQIYYRDTHGIRKSSKV
                     DEISYYVDEKGDFKAWDSSLSEHKIDRFVKLHLTDEEALDVYKSEEASKRGKYKGLFK
                     KTVFYENPLSDKDISRIKGMVDLRETYQSLIEIQRHQDYSRTDFQVLLSKLNHDYDRF
                     VSQFGYLNASVNRNLFDSDDKYSLLASLEDEYIDSKDQKVKYKKSLAFEKALVRPERV
                     ITRVSTALDALNSSLSDGRGVDLDYMVSIYPEHSQAAILDELGDQVLMDPESYLRGER
                     KYLSKNQFLSGDILNKIEVVQLLVEENNQEYDWNHALDLLESVRPPRIHLADIEFKIG
                     SRWIPQSVYGKFAFECFTNREFELSSPDVEQVIEVNPVDGQVHLRTSFAYRYPSAKDS
                     SLGVSGSRYDTGRKIFENLLNSNQPTITMTFTEGEKKKTITDLEKTSVLRAKEQHLQE
                     LFQEFVSRYPEVQQVIEESYNRLYNRTVSREYDGSHLVIDGLAQNISLRPHQENAIQR
                     IVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLSAQFGQEIMKFFP
                     TKKVFVTTKKDFVKARRKQFVSRIITGDYDAIVIGDSQFEKIPVSKERQMNYIEDKLN
                     ELREIKTHSENKYTVKEAEQSISGLEKQLEELQRFNRDSFIDFENLGIDFLFVDEAHH
                     FKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQIQEEHDFKNIVFATGTPVSNSISEL
                     YTMMNYIQPDILKRYQVDYFDSWVGAFGEIQNSMELAPTGDKYQPKKRFKKFVNLPEL
                     MKIYKETADIQTQDMLDLPVPEAHIIPIESELTENQKLYLEELVMRSDMVKCGTVDPS
                     QDNMLKITGEARKLAIDMRLLDSSYSLADNHKLLQVVDNVERIYREGMENKATQMIFS
                     DIGTPKKKDNGFDVYSEIKALLVDRGIPSKEIAFVHDANSDEKKNSLSRKVNAGEVRI
                     LLASTEKGGTGLNVQSKMKAVHHLDVPWRPSDIQQRNGRIIRQGNENKEVDIYHYITK
                     GSFDNYLWATQENKLRYIKQIMTSKEPIRAAEDIDEQTMTASDFKALATGNPYLKYKM
                     ELENDLTLLENQRRAFQRSKDHYRHTISYCEENMPILEKRLSKYEGDIQQSEMSKDQA
                     FSMTVGKQAFEQRAEAGESLHRLIRHNQSDSKEFRTLASYRGFDIKMLSLPTNQPLPE
                     TFSVKIVGENQYSVSLDLYSPLGTIQRLQHTIDHIKEDQVKTQNLLDELQDKLTTAKV
                     EIEKNFPKEEDYQTKKAEYDVLAPLIETETDLDIIDQALRQFHEKGKEKQEQLSFELD
                     "
     gene            7247..7531
                     /locus_tag="ACHBRQ_001762"
     CDS             7247..7531
                     /locus_tag="ACHBRQ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5962 family protein"
                     /protein_id="HFM9593379.1"
                     /translation="MMEDTYYQLEEALVQGFQTPEEYQAYKELKEHYEEVTGDYSFSK
                     RELTSQLEIALQNHRGVDFEDYEKEDYLELVQKLEEFDSSLATHYRQLID"
     gene            7545..7865
                     /locus_tag="ACHBRQ_001763"
     CDS             7545..7865
                     /locus_tag="ACHBRQ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5966 family protein"
                     /protein_id="HFM9593380.1"
                     /translation="MNDLLLIPVIFLAVGGILILLWRIFLIASGLFLIGFVSFLIFVE
                     VYGIYLFFTETELYTEDLAQNGLFGFTTFFIIFNLTLLVLAGWAGYKRKRHNNVLANQ
                     RDFW"
     gene            8576..>9343
                     /locus_tag="ACHBRQ_001764"
     CDS             8576..>9343
                     /locus_tag="ACHBRQ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593381.1"
                     /translation="MENNHSEVQKNKLTIDKVPVQNKKLTEALRVFHETLNHSRLIEV
                     QNSMLQANKAAETALASFNFSKQIAETTKPFLTEINSATSILNNQAYLKILSETEAAV
                     SSILLKTQIDNIGQILDPIHNIFDKSTFNFTESLKEFLKEYHAPYSEIEAEAITLNIE
                     KLAEEGWVIYHSYSDDYRTICTIDFEELIKEWQTLLKEDLEDKELIQELKDLTYYSEP
                     LIDSMIESYRSQNYYAAYTLATIAIDGALNRFSEPFSN"
BASE COUNT         3235 a         1465 c         1876 g         2767 t
ORIGIN      
        1 tctctttttc atgaacacaa atctcctcta atcgtctctc tgacttttta ggactgaatc
       61 accttcaaag accacgaaga cgactttttt gaatgtccat ctggttcatc atcttaggag
      121 gtttatccac tatgcttaga cgttttgtaa cccaaaagca tctcacgctc tactttctag
      181 cctagaatct ttaaaaatcc gaatcgaact attattattg cgacccacaa tcctcttatt
      241 tgggaacaag tggaccaagt tattcgagtt accgatttat cacatagatg atatggcaag
      301 attcagttag aagaaagttt ttgattatat acagcataaa tttctaaaat ctgtagtact
      361 tgtttataat cacaatatta tttgagaacg cagttttggc tcgttctctt tttttgttgt
      421 gatacttctt tcaaggaata tttggtagga ttggagtgaa aaaagattaa ctaggaaaga
      481 aggaagtata tggaagccat ttatcaacgt gattcggatc aagatggact gactgatgct
      541 caagaattgg cgctaggaac caatcctctt agcgcagatt ctgatggtga tggtcgttca
      601 gatttagtgg aagtagaaga aggaaccaat cccttagaaa aggatttaca agacatagac
      661 caaacaagta taactgaacc gtcttcagta tttatggaaa tgaaacaaaa gatttcagat
      721 atgatggaga gtcactacaa ggaatttata caggctctga ttagtattga aacagggatt
      781 gaaaaccaac aagacctaga agacttatat acttactaca tgagaacaga ttctgtttct
      841 cttttgtcta gtgatttaga aaccagtcct caagaggttg aaatggagat agagttgtag
      901 gggaatcatg tgattctcct attttcgtat agatgaaagg agcgagtatg gaagtaatgc
      961 aattattggc tatgtttcgt ggaacaattc caaaagatag ggagaaaatg gatctatttc
     1021 ttcgttatca agcgcaacat tttgatgaga aatggcagga tttggtagag aattttttga
     1081 ctgaagaggg caagatagaa gagattcctc acgtctattc ttttgatcaa gatattgttt
     1141 ctttcctaga ggctagttct gaaaataatg accaagattt agaaagttac acaagaagtt
     1201 ttgggcaagc gggtctaggt aaattatctc aattaagtaa ttttgagaaa aacttggtgc
     1261 tagaagtcgc aatctataac ctttccactc gattttacat acaatctgaa aaagaaaagc
     1321 taacaccatt aagtgagctt gtatgtcatc agaatcagga tgtcaattta gtcaatgtct
     1381 atcgggttgc gaataatcta tctgaccgca ttagtagaga tatagaggag tttcttctaa
     1441 tggttgattc caaagaggtt aaaaaagaag ttcttgagat tcattttgaa ggaaaagagg
     1501 gagatgttct agcctatttg ggttctgaat taatggctac tttagatagt gttacggaac
     1561 ttgtccatca tgaagaaaac taccaacaac tcccactgac acaaaagctg aagattatta
     1621 ctcactttga tgatgtaaaa gctaaaatcg aaaagtctaa gcgagtagcg gaagtggtct
     1681 tacctttaga taatattgag caggtagaag aaaatgtgga agttcattcc ctatctaaag
     1741 tagataaaat tgtagaagaa gctttgaggg aatatccaat cggttcacaa gtaagttata
     1801 gaggacaagt atttcagttg gtttcgattg aaaatgcaca gttaaatgac ttagttcgcc
     1861 tagagctatt caatgattcc aaccagttat ttgaagagaa tcctatttta tatttaaaca
     1921 gtttggaaga gattgaacaa gtattgtctc tggtagaact tgaaaaagaa gattcagaga
     1981 ttgagattga atcaccaagt gaaagccagg aaatagattt gttttcctat ctggaagaag
     2041 aaaatgaaaa agataaggat aaggaaacaa aacctttaat ttcaggtata gaagagacgg
     2101 aagtccccgt tcaagatttt gtttttccag atgatttaga ggacttttat cctaagacaa
     2161 atcgtgaaaa aattgaaacg aatatcgccg caattgaact tgttaaaaga ttagaaaaag
     2221 agggacgaca agcgaatcca gaagaacaag agctactagc caagtatgtc ggctggggtg
     2281 gtcttgccaa tgaatttttc gatgaactca atccaaagta tgaaacagaa cgtttaactc
     2341 ttaggagttt agtaagtaaa tcagaatact ccaccatgaa acaaagttct ctcacggcct
     2401 attatacaga cccaatgatt attcgccaga tttggcaaaa attactggat gatggttttg
     2461 ggggaggaag gatattagat ccttctatgg ggactgggaa cttctttgcg gctatgccta
     2521 gaagtatacg agataaatca gaactctatg gggttgaatt agacagtgtg acaggcgcaa
     2581 tcgcaaaaca actccatcca aatgtccata ttgaagtgcg aggatttgaa gaagttccct
     2641 atcaaaataa tagttttgat ttagtcttaa cgaatgtacc atttggaaat tttcgcattg
     2701 ccgataaaaa ctatgataaa ccttatatga ttcatgatta ctttgtcaaa cactcacttg
     2761 atttagtaag agacggagga caagtgtcga ttatctcatc tatcgggaca atggataaac
     2821 ggacagataa tgtcttacaa gagattaaat ccaacactca ttttttaggg ggagttcggt
     2881 tgccagatac ggcttttaaa aagattgcag gtacccgagt gactacagat ctcctcttct
     2941 ttcaaaagga ccaagcaaag aatcttaatg aggaggaact tgtctttagt ggctctattc
     3001 cctttgagga ggataagcgt gtctggatca atccttattt tgatgggaaa tacaatacac
     3061 aagttttggg taaatatgaa gtacgtaatt ttaatggagg aaccctcaat gttaaggggg
     3121 gatcagaaac attagctact gacataatga aagcattaga gaatgtggaa gaacctaaac
     3181 caattgataa ttctttgaaa gcacctgttt ttatccaaga agaagtggat aattcaatcc
     3241 caagtcgtat acgtgaggac ttagcgctct attcttttgg atatgaggga aatcaaattt
     3301 attaccgaga tacgcatggc attcggaaaa gttcaaaagt agacgaaatt agttattatg
     3361 tagatgagaa gggagatttt aaagcttggg acagttcttt gtctgaacat aaaatagatc
     3421 gattcgtgaa acttcatttg acagatgagg aagcactaga tgtttacaag tcagaagaag
     3481 cgagtaaaag agggaaatat aagggcttgt tcaaaaaaac tgtcttttat gaaaatccct
     3541 tatcggataa ggatattagt cgtattaagg gcatggttga tttgagagag acctatcaat
     3601 ccttaattga aattcaacgt catcaagatt atagtcggac agattttcag gtattactta
     3661 gtaaactcaa tcatgactat gaccgctttg taagtcaatt tggatacttg aatgcctcag
     3721 tcaatcggaa cttatttgat agtgacgata agtattcttt actagcaagt ttagaagatg
     3781 aatacattga ttctaaagat cagaaagtaa aatataaaaa atctttagcc tttgagaaag
     3841 cattggttag gccagagaga gtgattacaa gagtatcaac ggctttagat gccttaaact
     3901 ctagtttatc ggatggtaga ggggttgatt tagactatat ggtatcaatt tatcctgaac
     3961 atagccaagc tgctattttg gatgagttag gagaccaggt tttaatggat ccagaaagct
     4021 atttaagagg agaaagaaaa tatctttcta agaaccagtt tttatcagga gacattctca
     4081 acaagataga agtagttcaa ctattagtag aggaaaacaa ccaagaatat gattggaacc
     4141 atgctttaga tttgttagaa tctgttcgcc ctccacggat tcatctggca gatattgagt
     4201 ttaaaatagg gtcacgttgg attcctcaat ctgtttatgg taaatttgct tttgaatgtt
     4261 ttaccaatcg tgaatttgaa ttgtcttcgc cagatgttga acaagtcatt gaagtgaatc
     4321 ctgtcgatgg ccaggttcat ttaaggacat catttgctta tcgctatcca agtgcaaaag
     4381 atagtagtct tggagtcagt gggtcacgtt atgatacagg aagaaagatt tttgagaatt
     4441 tacttaattc gaaccaaccg acgattacca tgacttttac ggaaggagaa aagaaaaaga
     4501 ccatcacaga tttggaaaaa acctctgttc taagagcgaa agagcagcat ttacaagaac
     4561 tctttcaaga atttgtctca cggtatcccg aagtccaaca agtcattgag gaaagctata
     4621 atcgtcttta taatcgaacg gttagtcgag agtatgatgg tagccatcta gtcattgatg
     4681 gcttggcaca aaacatcagt cttcgtcctc accaagagaa tgccattcaa agaatcgtag
     4741 aagaaaaaag agccttgtta gctcatgagg taggttcagg aaagaccttg accatgcttg
     4801 gtgctgggtt taaattaaag gagttgggga tggttcataa gcccttgtat gtggtgcctt
     4861 ctagtttgtc tgctcaattt ggtcaagaaa tcatgaaatt tttccctact aaaaaggtct
     4921 ttgtgaccac taagaaagat tttgtgaagg cgagaagaaa acaatttgta tcacgtatta
     4981 ttacaggaga ttacgatgcc attgtcattg gggattctca atttgaaaaa atccctgtca
     5041 gtaaagaaag acagatgaat tatattgagg ataagctcaa tgaactacga gagattaaaa
     5101 cacattctga aaataagtat actgtcaaag aagcagaaca atcaataagt ggtctagaga
     5161 aacaattgga agaactgcaa cgctttaatc gtgatagttt tattgatttt gagaacttag
     5221 gaattgattt tctctttgtg gatgaagcac atcactttaa aaatattcgt ccgattacgg
     5281 gacttggaaa tgtagcaggg attaccaata caacctctaa gaagaacgtg gatatggaaa
     5341 tgaaggttcg acagattcag gaagaacatg attttaagaa tattgttttt gcgacaggaa
     5401 cacctgtttc caattctatt agtgagttgt atactatgat gaactacatt caaccggata
     5461 tcttaaaacg ctatcaagtt gattattttg actcttgggt aggtgctttt ggagaaattc
     5521 aaaactctat ggaattagct cctacagggg ataagtacca acctaagaaa cgatttaaaa
     5581 agtttgtcaa tctacctgag ttgatgaaaa tctataaaga aacagccgac attcaaacac
     5641 aagatatgtt ggatttacct gttccagaag ctcatattat ccctattgag agtgagttaa
     5701 ctgaaaacca gaaactctat ttagaagaat tagttatgag gtcagatatg gtcaaatgtg
     5761 gaacagttga tccgagccag gataacatgt taaagataac aggtgaggca cggaaactag
     5821 ctattgatat gcgtttattg gactctagct atagtctagc agacaatcat aaactgcttc
     5881 aggtagttga taatgttgaa agaatttatc gtgagggaat ggaaaataag gctactcaga
     5941 tgattttttc agatattggc acccctaaga agaaggataa tggctttgat gtctattctg
     6001 aaataaaggc tttattagtg gatagaggaa tccctagtaa ggaaattgcc tttgtacatg
     6061 atgccaatag tgatgaaaag aagaatagct tgtctcgaaa ggtcaatgca ggagaggtgc
     6121 ggattctcct tgcttcaact gaaaaaggag gaacaggttt aaatgttcag agcaagatga
     6181 aagcagttca tcatctggat gtcccgtgga gaccaagtga cattcagcaa cgcaatggcc
     6241 gtattatccg acagggaaat gaaaacaagg aagtggatat ttaccactac attacaaaag
     6301 gttcatttga taattatcta tgggcaactc aggagaacaa actccgttat attaagcaga
     6361 ttatgacttc taaggagccg attcgtgctg cagaagacat tgatgaacag actatgacag
     6421 cttctgattt taaggcacta gcaacaggta atccttatct caaatataag atggaattgg
     6481 agaatgatct aaccctatta gaaaatcaaa gacgcgcctt tcaacgcagc aaggatcact
     6541 atcgtcatac aatctcttac tgtgaagaaa atatgcccat tcttgagaaa cgattaagca
     6601 agtatgaagg cgacattcaa cagtctgaaa tgtcgaaaga ccaggcattt tctatgacgg
     6661 taggtaagca agcttttgag caacgagctg aagcaggtga atctctacat cgcctcatcc
     6721 gtcataacca atctgacagc aaagaattcc gtaccctagc aagctatcga ggatttgaca
     6781 ttaaaatgct tagtcttcca acaaaccaac ctcttcctga aaccttctct gttaagattg
     6841 taggagaaaa tcagtattct gtcagtttag atttgtattc tcctttgggg acaattcaaa
     6901 ggcttcagca tacgattgat cacattaaag aggaccaagt gaaaactcag aatttattgg
     6961 atgaattaca ggataaattg actactgcta aagttgaaat tgagaaaaat tttccaaagg
     7021 aagaggatta tcaaactaaa aaggccgaat acgatgtact cgcgccattg attgaaacag
     7081 aaacggattt agatattatt gatcaggcct taagacaatt ccacgaaaaa ggaaaagaaa
     7141 agcaagaaca actttctttt gaattagatt aaaaaataga tacaaatagc ggacaagaaa
     7201 agaaaagcgc ggtaaaataa agatagaaag aaacgaggta acgattatga tggaagatac
     7261 ctattatcaa ttagaagagg ctttggtaca gggatttcaa acacctgaag aataccaagc
     7321 ctacaaggag ttaaaggaac attatgagga agtgacaggg gattacagtt tttccaaacg
     7381 agaacttact agccaattgg aaatcgctct tcagaatcat cgaggtgtgg actttgaaga
     7441 ttatgagaaa gaggactatc tggaattggt tcaaaaacta gaagagtttg attcctctct
     7501 tgccacccat tatcgtcagt taattgatta ggaaggaaca agtcatgaat gatttactcc
     7561 ttatcccagt aattttttta gcagtaggag gaattctcat tcttttatgg aggatcttcc
     7621 ttattgccag tggacttttc cttattggtt ttgtcagttt tcttattttc gtggaggtct
     7681 atggaattta tttgttcttt acagagaccg aactttacac ggaagattta gcacagaatg
     7741 gattatttgg ttttacgact ttctttatca tctttaacct tactttgctt gtcctggcag
     7801 gctgggcagg gtataagcgg aagaggcata ataacgtttt agcaaatcaa cgcgattttt
     7861 ggtgagttct tcattttcgg tatcttccta tctattattt ttatgcttgg tacagtatta
     7921 gttatctact ataaatctct gaggattatg aagactgcaa acgctttatc ctcttgtaaa
     7981 atataggtct agatcaaaag caaatcttgg gagaatatcc tatatccaaa agttactaga
     8041 tataaagaat tgttgatgag gattcgatgg tggcgggtta accaatcact actagctcag
     8101 cttaactaaa atataaaaaa tagtctggga taatattccc ttatctccag aaaaagaaac
     8161 tgcaaatcca ttgctattta acatggttgc gatagtcttg gtaaaataga attgcccaat
     8221 aaaccattta cagatgccgc tttgatgccg ataaaagaaa accatatgaa gagtggtaaa
     8281 cttaaggcta aaactaaaaa tttttgtact ttagttaact cttgataaaa ctgggttaag
     8341 tcttaatata tactctttaa aaaatgtact ttattttaag gtcggataaa gtacattttt
     8401 tgatttagaa gtgttcaata agtatcattt ataaagtaca ttttggtaaa aagtattaat
     8461 actttcaatt gtacattttt aatgaaaata tttcttttct aatcattaca aaactcgtga
     8521 atgttgaaca cttagtggta taattaaaac atgacatata gaaagaaata gccctatgga
     8581 aaacaatcat agtgaagttc aaaaaaacaa attgacaatt gataaagtcc cagttcaaaa
     8641 caaaaagctt acagaagccc ttagagtttt tcatgagaca cttaatcatt caaggttgat
     8701 tgaggttcaa aatagtatgc tacaagcaaa taaagctgct gagactgcgt tagcaagttt
     8761 taatttttct aagcaaatag ctgaaaccac taaacctttt ctaacagaaa taaactcagc
     8821 cacgtctatt ttaaataacc aagcatatct taaaattcta tctgaaacag aagcagcagt
     8881 tagttcaatt cttttaaaaa ctcaaataga taatattgga cagatacttg atcctattca
     8941 caatattttt gataaatcca cttttaattt taccgaaagt ttaaaagaat ttttaaagga
     9001 ataccatgca ccttattctg aaatagaagc agaagcaatc actttaaata ttgaaaaact
     9061 tgctgaagaa ggttgggtaa tctaccatag ctatagtgat gactatagaa caatctgcac
     9121 tatagatttt gaagaattga taaaggagtg gcaaactcta ttaaaagaag atcttgaaga
     9181 taaggagctt atacaagagt taaaagactt aacatattac tcagaaccct tgatagattc
     9241 tatgatcgaa agttatagaa gccaaaatta ttacgctgct tatacgcttg ctactatagc
     9301 tattgacggt gcattaaaca gattttcaga accattttca aat
//
LOCUS       DAYYQN010000069         9026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYYQN010000069 DAYYQN010000000
VERSION     DAYYQN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.070"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..456)
                     /locus_tag="ACHBRQ_001765"
     CDS             complement(<1..456)
                     /locus_tag="ACHBRQ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM9593382.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPK"
     gene            complement(623..1426)
                     /locus_tag="ACHBRQ_001766"
     CDS             complement(623..1426)
                     /locus_tag="ACHBRQ_001766"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9593383.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1510..1731)
                     /locus_tag="ACHBRQ_001767"
     CDS             complement(1510..1731)
                     /locus_tag="ACHBRQ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593384.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(2210..2419)
                     /locus_tag="ACHBRQ_001768"
     CDS             complement(2210..2419)
                     /locus_tag="ACHBRQ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593385.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            2589..2861
                     /gene="rpsP"
                     /locus_tag="ACHBRQ_001769"
     CDS             2589..2861
                     /gene="rpsP"
                     /locus_tag="ACHBRQ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9593386.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            2881..3120
                     /gene="kphA"
                     /locus_tag="ACHBRQ_001770"
     CDS             2881..3120
                     /gene="kphA"
                     /locus_tag="ACHBRQ_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HFM9593387.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            3269..3592
                     /locus_tag="ACHBRQ_001771"
                     /pseudo
     CDS             3269..3592
                     /locus_tag="ACHBRQ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            3696..4445
                     /locus_tag="ACHBRQ_001772"
     CDS             3696..4445
                     /locus_tag="ACHBRQ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9593388.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSE"
     gene            4515..5033
                     /gene="rimM"
                     /locus_tag="ACHBRQ_001773"
     CDS             4515..5033
                     /gene="rimM"
                     /locus_tag="ACHBRQ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9593389.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            5023..5742
                     /gene="trmD"
                     /locus_tag="ACHBRQ_001774"
     CDS             5023..5742
                     /gene="trmD"
                     /locus_tag="ACHBRQ_001774"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9593390.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELVAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            5754..6092
                     /locus_tag="ACHBRQ_001775"
     CDS             5754..6092
                     /locus_tag="ACHBRQ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HFM9593391.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(6123..6443)
                     /locus_tag="ACHBRQ_001776"
     CDS             complement(6123..6443)
                     /locus_tag="ACHBRQ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593392.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            6541..6756
                     /locus_tag="ACHBRQ_001777"
     CDS             6541..6756
                     /locus_tag="ACHBRQ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9593393.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(6753..6899)
                     /locus_tag="ACHBRQ_001778"
     CDS             complement(6753..6899)
                     /locus_tag="ACHBRQ_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593394.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKAKLMRFEEI
                     FEEY"
     gene            complement(6961..7497)
                     /locus_tag="ACHBRQ_001779"
     CDS             complement(6961..7497)
                     /locus_tag="ACHBRQ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9593395.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7923..8261
                     /locus_tag="ACHBRQ_001780"
     CDS             7923..8261
                     /locus_tag="ACHBRQ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593396.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            8327..>9026
                     /locus_tag="ACHBRQ_001781"
     CDS             8327..>9026
                     /locus_tag="ACHBRQ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593397.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDL"
BASE COUNT         2917 a         1494 c         1847 g         2768 t
ORIGIN      
        1 ctttggaagc cgagaaaaat catgctcaaa gtgaaagtca ttgagcttgc gaatgacagt
       61 tgaagttgaa atggccagct gatgggcaat atcagtcata gaaatttttt caattaactt
      121 ttgagcaatt ttttggttga tgatacgagg gatttggtga tttttcttta ccaggggagt
      181 ctcagcaacc atcatttttg aacagtgata gcacttgaaa cggcgttttc taaggagaat
      241 tctagaaggc ataccagttg tttcgaggta agggatctta gacggttttt gaaagtcata
      301 tttcttcatt agacttccac aatcagggca agatggagcc tcataatcca gcttagcgat
      361 aatttctttg tgggtatcca tattgatgat atctagaatc ttgatgtttg ggtctttaat
      421 atcgagcagt tttgtgataa aatgtaattg ttccatatga atctttctaa tgagttgttt
      481 tgtcgctttt cattataggt catatgggac tttttttcta caataaaata ggctccataa
      541 tatctatagt ggatttaccc actatagata ttatagagcc agaaatacag taccgaatgt
      601 ggatactgta tttttttggt ttttaagatt taaaatcgta gtctttcacc acttcgatgc
      661 tgtcgattgt gatagcagta gttggcttgt ctttttcatc tttttcggct ttagcaatct
      721 tatccacaac atccataccg tcaatcactt gaccaaagac tgggtgtttg ccatctagac
      781 tagggtttcc accttctttg taggcttcga taattttctg tggatacttg cttgtaggga
      841 gtttagaaga ggtatctgta gagttttggt tgatgaagaa ctggctgcca ttggtgtttg
      901 gttgaccagt attagccata gcaagagcac cacggatgtt atacaaataa ggagtaatct
      961 cgttcttgaa accagttcct ttgtctttag tcttatcctt gtcatgccat atggactgac
     1021 cacctgtacc gtcccctttt ggatctccag tttggaccat aaagccatcg atgacacggt
     1081 ggaaggtaat accgttatag tagccttctt tggcgtgagt gaggaaattt tcaaccgcta
     1141 gaggagcgag tttagggaag agtttaatgc gaatatcacc ttggcttgtg tggagaatca
     1201 cttcggcttc atcttcagca acttccttag atagttgtgg gaaattggcg ttttcgtttg
     1261 ttaaagcatc gtttaactcc ttggcagatt gggcagctac tttggaactt tcttcagcag
     1321 caagactgga atcaacataa tcatcaccac gcagactgcg ttggacgctg ctacacccag
     1381 ctagggctac agtagacagt aaaagaaggg ttgctagttt tttcattctg atcctctcaa
     1441 aaattcattt ataaccattg taccctaaaa ggaagtggat ttcaaatatt agtagtttac
     1501 agaactggct tagataatag ttgggatttc atcatcatct tgaaaatctt caacttcttc
     1561 aacttcttcc ccagtaaatg gtttctgaaa agaacttagt ataaaacggg caattggttc
     1621 aacaattagg aaatttagag gaagagctgc aataaagttt acgaaccagg cgcttagata
     1681 ggtattgggt gtgatagcaa tgccattgta aaaaagtcca tagaaggaca tgaaagtgac
     1741 cagctaattc ttcttctgaa aaatgctcat gaagggcttg atccaggtcg caagatttca
     1801 ttttagaaag tccagtaact cccttcttct tcaggatatt ctttttcatg gtagtattta
     1861 gatggtcaag tgagtcaaaa gaagtttcga caagagcata ccctttttct accagagttt
     1921 ttagatgttt aggggcatca ataccataag tgtactcaaa gtattttgga aaccagatct
     1981 taagaaagct cgtaaagcta gccagttctc taaacgctaa gaactagcta caacacagca
     2041 tttacaacac ttcatggttc acaccatggg gtgtttttta atgattttaa gcaaaattca
     2101 cacttaattg aaagttattt aaattagatg aaaatttatt ttttgaaaaa gtgggaaaac
     2161 attgattcta agcgaatttt gaatcatatt cgaatgggag tttttaaaat catgtttcag
     2221 tggtaaaagt accaaactgg attctccata gtaaaatgat atctcctgct agtgaatcat
     2281 ctactaataa gtccatattt cgcttgggaa cgggcttggg acttagaagc caagtatcca
     2341 actctcgttc aggcgatatg aagggtttga cttggtgttc tttataaaat ttttctaaaa
     2401 tagtattcac ggatagcatc tttcaaacat ttatttccta ttctaacaaa tttctaaata
     2461 ctttaagaaa tttatagtct gtcaagtttt tttcttgaca catgccagaa atctgctata
     2521 atagaacatg tgctagatag ctcagctatt tcaccgaaat aaaaaattaa agaaatgaga
     2581 ctttaaaaat ggcagttaaa atccgtttga ctcgtatggg ttctaagaaa aaacctttct
     2641 accgtatcaa cgtagcagat tcacgttcac cacgtgacgg acgtttcatc gaaacagttg
     2701 gaacttacaa cccacttgtt gctgaaaacc aagtaacttt gaaagaagac cgcgttcttg
     2761 catggttggc taatggagct caaccttcag acacagtacg taacatcctt tcaaaagaag
     2821 gcgtattgaa aaaattccac gattctaaat tttcaaaata agtttaaagt aggttgacag
     2881 atggatacga ttgaaaatct cattattgcg attgtgaaac ccttaatttc acaaccagat
     2941 gccttaacta tcaagattga ggatacacca gaatttttgg aatatcattt gaatcttgat
     3001 caaagcgatg tgggtcgtgt aatcggtcgt aagggtcgca ctatttctgc gataagaacg
     3061 attgtctact ctgtcccaac tgaatacaaa aaagtaagaa tcgttattga cgaaaaataa
     3121 gaagggcggg acggatgtct cgcttttttg tgtggaatag aagatatagt aaaatgaaat
     3181 aataacagga caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtaa
     3241 aggtgtacta ttctagtttc aatatactat aaaagattga acgtttaggc aattccaaga
     3301 ctacttactc gaacattaac agcagtctcg gactgatgaa ggtctcttta tgaagttagt
     3361 ggagaagtcg gagaagtagc cgaggttttg aataggcgct ctggtcgaaa agaagggata
     3421 ggattcgaac gaggaacttg ccagagaact ggctgatatc atttactaca ccgtcgcaat
     3481 cgtagctatt aatcatattg accttaccaa gactgtcttt gagaaagaca aaaccgcagc
     3541 cgttaattac caacataaac atgatttgga aggatttttg aagggaaagt agagatgttg
     3601 tccccttcct ccaacttgag aaaatccttg gaacgtgata aaataaaaga taaggatttt
     3661 taataattca ttatatagaa tgagtggatt tctttatgaa gaggtttgaa gtatctacag
     3721 aaattggtag cctctctgtt acttatcaaa agcaaaagaa agtgctagtt tgtctaaacg
     3781 gagcaggttt gctaccaagt tatgaaaatt tttcacttat acttgaaaaa cctcctccta
     3841 caattggtta tttgacaatt gattttccga acacaggtag gagtccgatt catgaccaag
     3901 ctggaaaaaa tctagataat cttgcagatg cggtttatga agtacttgaa gaattaggaa
     3961 tttctgaata tatactttgt gcacatagtt ggagtggaat tttagcttgc aaattactcg
     4021 agaaaccaat taagcgtcag actttagtag caattgaacc gacaactaaa aaagtcatgt
     4081 ttgctgattt ttcagaaaat ccttatccag aaatggaaga gcagatgagg ctgattgacg
     4141 agtgtggtcc tgaactttat tttaagaact taactcaagc aacatttagt cctgaaacga
     4201 ataaaaaaat ctgggaatta atgcaagaaa aaggcttaga gttggaaaat caagatccag
     4261 aatttcagat atctggagag attactgagg aagattttga gaatttgtcg atagaatctc
     4321 atgtccctgt atttattttt tgtcagactt atagagaaaa agagtacaga gaatcagaat
     4381 attggacttc caatactaaa ctcattttag gaaggaatca ccattattta caatggtcag
     4441 aataggaaaa aattgcggct attattcgag aattgtcaga ataagatgga aaaaaggaga
     4501 ttacaggaga caagatgaac tactttaatg ttgggaaaat cgttaatacg cagggattac
     4561 agggtgagat gcgagtcttg tctgtgacgg attttgcaga agaacggttt aaaaaaggag
     4621 ctgagctggc tttgtttgat gaaaaagatc agtttgtcca aacagtgacc atcgctagcc
     4681 accgtaaaca gaagaacttt gacattatta aattcaaaga tatgtaccat atcaatacta
     4741 tcgaaaagta caagggatac agtctcaagg tcgctgagga agatttgaat gacctagacg
     4801 atggtgaatt ttactatcac gagattatcg gtttggaagt ctatgagggt gatagcttgg
     4861 ttggaaccat caaggaaatc ctgcaaccag gtgctaatga tgtctgggtg gtcaaacgaa
     4921 aaggcaaacg tgatttgctt ttaccttata tcccaccagt ggttctcaat gttgatattc
     4981 caaataaacg ggtcgatgtg gaaatcttag aagggttaga cgatgaagat tgatatttta
     5041 accctctttc cagagatgtt ttctccactg gagcactcaa tcgttggaaa ggctcgagaa
     5101 aaagggctct tggatatcca gtatcataat tttcgagaaa atgctgaaaa ggcccgtcat
     5161 gtagatgatg agccctacgg aggcggtcag ggcatgttgc tcagagcaca acctattttc
     5221 gattcctttg atgctattga aaagaaaaat ccgcgcgtta ttctcctcga tcctgctgga
     5281 aagcagtttg atcaggctta tgctgaagat ttggctcaag aggaagagct aatctttatc
     5341 tgtgggcact atgagggtta tgatgagcgc attaagacct tggtaacaga tgagatttcc
     5401 ctaggcgact atgtcctcac tggtggagaa ttggtagcta tgaccatgat tgatgctaca
     5461 gttcgcctga ttccagaagt gattggcaag gagtctagcc accaagatga tagtttttct
     5521 tcaggtcttt tagaatatcc tcagtacaca cgtccctatg attatcgagg catggtcgtg
     5581 ccagatgtat tgatgagtgg ccaccatgaa aagattcgtc agtggcgatt gtacgagagt
     5641 ttaaagaaaa cctacgagcg cagaccggat ttacttgaac attatcaact gacagtagaa
     5701 gaagaaaaaa tgctggcaga aatcaaagaa aacaaagaat aaaggagaaa cctatgcaag
     5761 taatcaaacg taatggcgaa attgctgaat ttaatccaga taagatttac caagccatct
     5821 tgaaggcagc ccaaactgtt tatgttttga cagatgattt gcgtcaaaat cttgctcaag
     5881 tcactaagaa ggtagttttg gatttacaag aagccaaggt ggaacgtgcg actatcagta
     5941 tgattcaatc tatggttgaa catcgtttat tgggcgcagg ttacattacc attgcagaac
     6001 actacatttc ctatcgttta caacgtgact tggaaagaag tggttatgga gatcatattg
     6061 cagttcattt acattttgaa caaattcgct aagaaaaaag agtgggatga agcaactaca
     6121 tctcactctt tcttaaatct atttttttgg gctgtttgga cagttgaggg gacaaatcgc
     6181 aggcgttgaa tatcgcttat acggataatc cggctcacat ttttggcaaa atttttcacg
     6241 ataatttgct ggccttgctg atcatatttg atgatatcac ctgtaaaact tgtctcagac
     6301 aagataaggt gaacagcggt ttgtttctgg atagcctctt caatagtggc tgtaagggag
     6361 ttggtttcag tctggtcagg ttggtcatgt ccatttaaaa aatcatgtat tttatctaga
     6421 acttgctgga atttatgtct catattggcc tcccttcttt tatatcaata ttatataaaa
     6481 ttttcctaaa atctacaaga ttttacgaat aaactaatga aactaaaaaa ggagaagaaa
     6541 atggcagaat ttacatttga aatcgaagag cacttgttga ctctttctga aaacgaaaaa
     6601 ggttggacca aggaaattaa ccgtgtgagc tttaatggtg cccctgcaaa gtttgatatt
     6661 cgtgcttgga gtccagacca tactaaaatg ggcaaaggga ttactctctc aaatgaagaa
     6721 tttcaaacga tggtggatgc ctttaaaggc aactaatact cttcgaaaat ctcttcaaac
     6781 cgcatcagct ttgccttgcc gtatgtatgg ttactgactt cgtcagtttc atccacaacc
     6841 tcaaaacagt gttttgagca gcctgcggct agcttcctag tttgctcttt gattttcatt
     6901 gagtataagt tttcaaaata aaaaaaagga tattgagtca tgataactcg gtatcctttt
     6961 ttagttagta aagtaagcct gatttttaag gcgttgaagt aggggacggc taataaaact
     7021 aatagccaga agtttgccaa ggtctggaat gataaaggga agaaccccca caacaagagc
     7081 tttttcaaat gccattccag ctaggaaatg caagctgaga atcccgccga caaagacaag
     7141 ggcatcaccc aagaggtttg caagaaaaat cttaacaaca ccactcttgc tgttggttag
     7201 agaggaagta agtccagagt aaacgagata aaaccaaaga tagcctgcag tagggccaac
     7261 taaagcctga aaaccagctc cacctcctgc aaagacagga agaccgatag cacctagaag
     7321 aagatagagt ccagcagaaa gtacagcctc tctcggtcta aagacagtag caatcaagcc
     7381 gattgcaaag ttttgcagag tgaagggaac aggtccaatt ggaagactga tttgtgccaa
     7441 aacagcaatg agagcagccc caatagcagg gatagcataa acgtgagctt ttttcaaaat
     7501 agttaacctc ttttctaatg tatagtaact attatactca tataggagtc gatgtcaacc
     7561 attttttaaa aacaaggtaa caaaaactgt aacagccgat tttcgtgact agtcaagttg
     7621 gataattttt ttgttgattc gaaaattgtc tgtatagata atcctatttt attaataaaa
     7681 aatatactag taaatacttg acaaattaaa aaataagtgt tacattaaaa atgtaaacgg
     7741 atacatttag atgtgttcaa ttttaaaagg aggggtagtt atgagatatt ttaacgactc
     7801 tagataccat aacggtgatt aaaaacctaa atataatcaa ttcatattaa agtaatatgg
     7861 agggactaaa aagagactat tatttaatct tcatcaaata tttaaattca aggagaagta
     7921 aaatgaaaaa aagaattaat aagaaagcat ctgtttttct agttgcaagt gttttcagtt
     7981 ttagctttgc tactacagct agtgtcgtta acgcagtaac ggcacaccct gctccaagtc
     8041 ccattattga tgtatgggaa tatggtgtaa atgataacta tggttattcg aattactttg
     8101 ttagttctcc aagtaatatt ggatcaaaat cttcagtaac gaatttttgg ggaacagtta
     8161 aagctcagga taaaaaagat tatggatggg catattcaag tgcaacaaaa tcttggaacg
     8221 atgttcgctt aaatgcatat tgggattatt atagatttta aaaataattt atgaaaacaa
     8281 gagacccaat tataagattg ggtctcttgt aatatggtaa ttgcttatga aattaaaatt
     8341 atgtatcatt ggattcttct tttgtttgat tgctacaatt ggtctggtca ctattagtga
     8401 taccgaaatc cccatacctt taccaattga tggagctttt tctatccaag gaaaaagtaa
     8461 tctttctaat aacgaaatat acgaaatggt tcgtgactta tcaaaaacgg aaaaggttac
     8521 catttacaag ccaatcgttc agagttcggg tcagttgaag tatgtaaatt ttgatgatgt
     8581 aaataatgaa caattaaagt cagcaccaat agtagggatg tattacacac ttgggaaaat
     8641 ggatgtagac agtttgaaac cacttacgat gacgggacta caaacagtct atatggctta
     8701 tccttggtat ataggaggaa tattacaatt cactggaact ttaagaatac tgttaatggg
     8761 ttcaatttat ttaactttat tagttgtttt atttgttgtt aggacgaggc agatcaaaga
     8821 aggagtgata cgacgttctt tgggcttgcc tatatacgac ttaagaagag agtatggcat
     8881 ttctcttatt tttgaactga ttatgatggc tttattgatg atttcttaca gttctttttt
     8941 gggtaatggt ttcttcacct atagttctaa gttatttttc tctctgcttt taacgaattt
     9001 tatactattt caaatcattg atctta
//
LOCUS       DAYYQN010000070         8996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYYQN010000070 DAYYQN010000000
VERSION     DAYYQN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.071"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            12..821
                     /locus_tag="ACHBRQ_001782"
     CDS             12..821
                     /locus_tag="ACHBRQ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9593398.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            826..1527
                     /locus_tag="ACHBRQ_001783"
     CDS             826..1527
                     /locus_tag="ACHBRQ_001783"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9593399.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1552..2469
                     /locus_tag="ACHBRQ_001784"
     CDS             1552..2469
                     /locus_tag="ACHBRQ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFM9593400.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2497..4038
                     /locus_tag="ACHBRQ_001785"
     CDS             2497..4038
                     /locus_tag="ACHBRQ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFM9593401.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4057..4515
                     /locus_tag="ACHBRQ_001786"
     CDS             4057..4515
                     /locus_tag="ACHBRQ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFM9593402.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4517..6739
                     /locus_tag="ACHBRQ_001787"
     CDS             4517..6739
                     /locus_tag="ACHBRQ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HFM9593403.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6779..7888
                     /locus_tag="ACHBRQ_001788"
     CDS             6779..7888
                     /locus_tag="ACHBRQ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFM9593404.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDCYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8045..8935
                     /locus_tag="ACHBRQ_001789"
     CDS             8045..8935
                     /locus_tag="ACHBRQ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9593405.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
BASE COUNT         3021 a         1337 c         1804 g         2834 t
ORIGIN      
        1 aaggagctca tatgttactg caaaaagaac taattccaat gatagaagct aacttaccaa
       61 atatggcata tgctgaaaaa gacattgcta aattcttctt aaaacagcaa cctctgaatg
      121 attattcatc taaagcattg tgcgaatacc ttaatgtatc caaagcaaca ttgactcgat
      181 ttgcgaaaaa atgtggtttt aaaggtttta gacaattcat tttcaaatac caagagatga
      241 ttcatgagaa agaaaagttg gcattatata cagaggcaac agaaaaagtt ttatccgact
      301 atgaggaaat gttgagaaaa acttacacgg ttcttgatga agttcaactt gagcgtattg
      361 ctgagatgat agaaactgct gagcgtgtat atctctacgg taaaggaagt tctgttcttg
      421 ctttacaaga aatgaagatg agatttatgc gtctcggagt gattggtgaa gtattatcag
      481 acgaggatat gattttgtgg agtagcttac tacttaatga aaattgcctt gtcattggag
      541 catccatttc aggtcaaact gatattgtac tagaaggtct acaaaaagct gcagataaag
      601 gcgctaaaac agttttaatg actacaagaa aatttgacga agaagattgt ttctttgatg
      661 aactattgtt attagcttcg accgatcatc tctcgtatgg caatcgcata tcacctcagt
      721 ttccaatact tttaattaca gactgcttat tctctaatta tctggaaagt ccagagagac
      781 aatattatta caatcaaact attatccata aggaggaata ataaaatgag ccaaatttgg
      841 actaaagaaa aatttataag ccaagttcaa ggtggagtca ttgtttcttg tcaagcttta
      901 cctggtgaag ccctttataa tgaagaattt agcttgatgc cttttatggc taaagcagct
      961 ttagaggcag gagcagtggg cattcgcgca aattctgtgc gtgatattaa agcaattcag
     1021 aaagtagtag atttaccaat aattggaatt atcaaaaggg attatccacc tcaagaacca
     1081 tatattactg ctacgatgaa agaagtagat gaacttgtag aatgcggaac aacagtcatt
     1141 gcatttgatg caactttaag accaagatat gatggcttag ttgtcagtga atttatcaaa
     1201 aaaataaaag aaaaatatcc gaatcaattg ctgatggcgg atgtaagtaa tttagatgaa
     1261 ggtctctatg catttaaatc aggcgttgat tttgttggta caacattatc aggttacaca
     1321 agtacaagtg tacaatcaga tgagcctgat tttgaactaa tgaaaaaatt ggctgatttt
     1381 aatattccgg taattgccga aggaaaaatt cattatccag aacaattaaa aaaagcttat
     1441 agtttaggtg ttaccagtgt agtcattggt ggagcgatta cacgtccaaa agaaattgct
     1501 cagcgattta ttaatgtcat caaataaaaa acaatataaa ggagaaatct gatgaaagat
     1561 ttaactaaat acaaaggcgt tatccctgca ttttatgctt gctatgatga aaatggtgaa
     1621 attagccaag atcgtgtaaa atctctggta caatatttca ttgacaaagg tgtaaaaggt
     1681 atctatgtaa atggttcttc aggtgaatgt atttaccaaa gtgtagaaga tcgtaaacaa
     1741 attattgaag ctgttatgga agttgctaaa ggtaaattaa cagttatcaa ccatattgca
     1801 tgtaataaca cgaaagatag tatcgaattg gcaaaacatt cagaaagtgt tggagtcgat
     1861 gctattgcag ctatcccacc tatttatttc aaattgccag agtattcaat cgcagcatat
     1921 tggaatgcaa tgagtgaagc tgcgtcaaat acagatttta ttatctataa tattccacaa
     1981 ttggcagggg ttgcgttgac tggtagtttg tatgcaacaa tgcgtcaaaa tcctcgtgtg
     2041 attggagtta aaaattcttc tatgcctgta caagatattc aaatgtttgt agctgcaggt
     2101 ggagaagatt acattgtatt caatggtcca gatgaacaat atcttggtgg tcgcttgatg
     2161 ggagcagaag ctggtattgg tggtacttat ggcgttatgc cagatttgtt cttgaaattg
     2221 gaaagtttga ttcaagaacg agatttagat acagctaaaa aacttcaata tgctatcaat
     2281 gaagttatct ataagatgat atcaggtaag gcaaatatgt atgctgtagc aaaagaagtt
     2341 ttgcgtctaa atgaaaaact tgatttaggt tctgttcgtc aacctttaga agcattggca
     2401 gaaggtgact tggaagttgc aaaacaagca gcagaactta ttcaacaagc acgaaaagaa
     2461 tttttataat aaataaaatc aatatggaga tatgttatgg gcacaacagg atttacaata
     2521 attgacttaa ttatcttgat tgtttattta cttgcggtgt tggttgcagg tatctatttc
     2581 tctaaaaaag agatgaaagg aaaagagttc tttaaaggag atggttcggt tccttggtat
     2641 gttacttcgg tatccatttt tgccacaatg ctcagtccga tttccttctt gggactcgct
     2701 ggtagctctt atgcaggtag ctggatttta tggtttgctc aattagggat ggtagtagct
     2761 attccactga caattcgttt tatcttacct atctttgcac ggatagacat cgatacggca
     2821 tatgattact tggataaacg ttttaattct aaagcacttc gtattatttc agcactcttg
     2881 tttattattt atcaattggg acgtatgtct atcattatgt acctcccatc agctggttta
     2941 tcagtattga caggaattga catcaatatt ttgattattt tgatgggtgt agttgcaatt
     3001 gtttattctt atactggtgg tctaaaatcc gtattatgga cagactttat tcaaggtgtg
     3061 attctgatta gtggtgtcgt tttagcttta tttgtactga ttgctaatat taaaggtggc
     3121 tttggtgcag tagcagaaac attagcaaac gggaaattcc ttgctgcaaa tgaaaaactt
     3181 ttcgatccta acttgctttc aaactccatc tttttaattg tgatgggttc aggctttaca
     3241 atcttgtctt cctatgcttc atctcaagat ttggttcaac gttttactac aacacaaaat
     3301 attaagaaac ttaataagat gttgttcaca aacggtgttt tgtcacttgc aactgcaaca
     3361 gtcttttact tgattggtac aggcttgtac gtattctatc aagtacaaaa tgcagatagt
     3421 gcagctagca atatccctca agaccaaatc tttatgtact ttattgcata ccagttacca
     3481 gtaggtatca caggtttgat cttggcagcg atttatgcag catctcaatc aactatttca
     3541 acaggtttga actctgttgc aacttcatgg acattggata ttcaagatgt catttctaaa
     3601 aatatgtcag acaatcgtcg tacgaaaatt gcacaattcg tatctctagc agtaggttta
     3661 ttctcaattg gtgtttccat tgtcatggct cactcagata ttaaatctgc atacgaatgg
     3721 ttcaatagtt tcatgggact tgtacttggt ctacttggtg gtgtatttat tcttggattt
     3781 gtttctaaaa aagcaaataa acaaggtgct tatgcagcgc tgattgtatc aaccatcgtc
     3841 atggtattta ttaaatactt ccttcctcca acagctgtta gctactgggc atattcattg
     3901 atttcaatct ctgtatcagt agtttcaggt tatattgtat ctgttcttac tggaaataaa
     3961 gtatctgcac ctaaatatac aacgattcat gatattacag aaattaaagc ggattcaagt
     4021 tgggaagttc gtcactaata gaaaagagaa aataatatga ttatttctga acaatctgat
     4081 tttaaacgat atgcttctgt caataaatat ttttcaaaag tctgtgattt tttagaaaat
     4141 acaaatttga cagatttagt tgatggaaaa attgatattg acggggaaaa tgtttttgca
     4201 aattgtatga cttatctagc tgatggagtt ccaggagata tttttgaaac tcataagaaa
     4261 tatttagata ttcatattgt tgttgaaaat actgaaaaaa tggcagtaac ttccccagtt
     4321 cgtgcccagt cacgtgtacc ttttagcgaa gagaaggata ttgcttttta cgatagcaaa
     4381 gactatcaaa ttgttgaatt gcttccaggg aatatgttgg tgacttttga agaagatctt
     4441 caccaaccaa agattcattg caatgatgaa acggttaaaa aacttgttat caaagtttta
     4501 aacgaggaaa aataaaatga aaaaaaatat taaacaatat gtaaccttag gtactgtagt
     4561 ggtattatca gcatttgttg ctaactcagt tgcagctcag gagactgaaa cttctgaagt
     4621 atcaacacca aagttggtgc aacctgttgc accaacgact ccgatttcgg aagtacaacc
     4681 tacatcggat aactcttcgg aagttactgt acaacctcga acagttgaaa ctactgttaa
     4741 ggatccatct tctacagcgg aagaaactcc tgtcttagaa aaaaataatg ttactttaac
     4801 agggggcgga gaaaatgtta ctaaagagtt aaaggataaa tttactagcg gtgactttac
     4861 tgtagtgatt aagtacaatc agtcaagtga gaaaggctta caagctctgt ttggaatatc
     4921 taattccaaa cccggtcaac aaaatagtta tgtagatgtg ttccttagag acaatggtga
     4981 gttggggatg gaagcgcgtg atacttcttc caataaaaat aacctagtat ccagacctgc
     5041 ttcagtttgg ggtaagtaca aacaagaggc tgtgactaac actgttgcag tagtagcaga
     5101 ttcagtcaaa aaaacatatt ctttatacgc aaatggtaca aaagtagtag aaaagaaagt
     5161 ggataatttc ctaaacatca aggatattaa aggtattgat tactatatgc ttgggggagt
     5221 gaaacgtgca ggaaaaacgg cgtttggttt taacggaaca ctagaaaata tcaaattctt
     5281 taatagtgca ttggatgaag aaactgttaa aaagatgaca acaaacgctg ttactggaca
     5341 tttaatttat acggctaatg atacaacagg ttctaactat ttccgtattc cagttctgta
     5401 tacttttagc aatggtcggg tattttcaag cattgacgct cgttacggtg gaactcatga
     5461 tttcttgaat aaaattaata ttgctacaag ttatagtgat gataatggta agacatggac
     5521 taaaccaaaa ttaacattgg cattcgatga ttttgcgcca gtaccattag aatggcctcg
     5581 tgaagttggt ggacgtgact tacaaatcag cggtggtgca acctatattg actctgttat
     5641 tgttgaaaaa aagaacaaac aagtactcat gtttgctgat gtgatgcctg ctggagtaag
     5701 ttttagagaa gcaactagaa aagattcagg ttataaacaa attgatggta attattacct
     5761 taaattaagg aaacaaggtg atactgatta caattatact attcgtgaga atggtactgt
     5821 atacgacgat cgtaccaaca gaccaactga attttcagta gataaaaatt tcggtattaa
     5881 acaaaatggt aattatttga cggtagagca atattcggtt tcatttgaaa ataataaaaa
     5941 gactgaatat cgtaatggga ctaaagtcca tatgaatatc ttctacaaag atgcattgtt
     6001 taaagtagtg ccaacgaact atattgcata tatttctagc aatgatcatg gcgaatcttg
     6061 gtctgcacca actttattac ctcctataat gggacttaat cggaatgcgc catatttagg
     6121 tcctggacgt ggaatcattg aaagctcaac tggacgtatt cttattccgt cttacactgg
     6181 taaagagtct gcgttcattt atagtgacga taatggagca tcttggaaag ttaaagtagt
     6241 gccacttcct tctagttggt cagcagaagc acaatttgta gaattgagtc caggagtaat
     6301 tcaagcatat atgcgtacaa ataatggtaa aattgcatat ttaacaagta aagacgcagg
     6361 tactacttgg agtgcaccgg aatatttgaa atttgtttca aatccaagtt atggaacaca
     6421 attatcaatc atcaattata gccaattgat tgatggtaaa aaggctgtca ttttaagtac
     6481 tccaaactcc acaaatggtc gtaaacacgg acaaatttgg attggtctaa ttaatgatga
     6541 taatacaatt gattggcgtt atcatcacga cgttgattat agtaactatg gatactcata
     6601 ttcaacattg acagagttac caaatcatga aattggattg atgtttgaaa aatttgattc
     6661 atggtctcgt aatgaacttc atatgaaaaa tgttgtacca tatataacat ttaagattga
     6721 agatctgaaa aagaattaaa gctgaaattt gaaaatatat aaaaagagga taaaaattat
     6781 ggtaaattac ggtattgttg gagctggata ttttggagct gatttagctc gctcaatgaa
     6841 caaaattgaa gatgcaaaag tggttgcggt atttgaccca aatcatggag aagaagttgc
     6901 tcaagagttg ggatcagatg tttgtgcaag tttagatgaa cttgtagcac gtgaagatat
     6961 tgattgtgtg atcgtagctt cacctagcta ccttcaccgt gaaccagttg tgaaagctgc
     7021 tcaacatggc aaacacgtat tttgtgaaaa gccaattgca ttgtcttatg aagattgtaa
     7081 agccatggtt gacgcatgta aagaaaataa tgtcatcttt atggctggtc acatcatgaa
     7141 cttctttaac ggtgtacacc atgctaaaga attgattact caaggtaaaa tcggtaaagt
     7201 tctttattgc catgctgctc gtacaggttg ggaagaacaa caaccaactg tatcatggaa
     7261 gaaacttcgt tctcaatctg gaggacattt gtaccaccat attcatgaat tagattgcat
     7321 tcagtttatc atgggaggac ttcctgaaaa agcgacaatg gtaggaggca atgtatatca
     7381 taaaggtgaa aactttggtg atgaagatga tatgctcatt gtaaacttag aatactctga
     7441 tgattgttat gctgttttgg aatatggtaa tgctttccgt tggggtgaac actacgtctt
     7501 gattcaagga actgaaggag ctatcaaact tgacttgttc aatactggcg gtactcttcg
     7561 tgttaaaggt gaaggagaat cacacttctt agttcatgaa actcaagagg aagatgatga
     7621 tcgtacagct atctataccg gtcgtggtat ggatggagca attgcgtacg gtaaaccagg
     7681 agtacgttgc ccattatggt tgcaaacatg tattgataaa gaaatggaat atctacatga
     7741 catcattaaa ggtggagaaa ttacagaaga atttgaaaaa cttctcaatg gtgtagctgc
     7801 tttagaatca atcgctaccg ctgatgcatg tactttatca gttaaagaag atcgaaaagt
     7861 aagtctttca gaaatcacaa atgcttaact tttgtaaaac agaatagtaa attcttgtca
     7921 ttatataatt tctaaagttc tgtgatacaa ctcattgaat aaagaaatag agatgggact
     7981 gggataatgc ccagtcccat tttttatcaa aaagtaatga gatcaaaaat gtgggagtgt
     8041 tgaaatgaag attataggta tcgatattgg cggaacaaca attaaggcag atttatacga
     8101 tgagtttgga acgagtttga atcatttcaa agagatagaa acaattattg actatgattt
     8161 gggaacgaat cagatattaa atcaggtctg tgatttaatt ggtgagtata ctttaaatca
     8221 ttcaattgat ggtgttggga tttccactgc tggagttgtt aatgctaata ctggagaaat
     8281 catctatgca ggctatacaa taccagggta tatcggagta aactttactg ccgaaataga
     8341 aaaacgtttt gggttgtata cttttgttga aaatgatgtt aattgtgctg cattaggtga
     8401 attgtggaag ggacaagcca aagataagaa aaatgtagta atggttacta ttggaacagg
     8461 tataggaggc agtattattg tcaacggaca aattgttaac ggatttaact atactgctgg
     8521 tgaagtaggt tatattcctg taggtaattc ggattggcaa agtaaagcct caacaaccgc
     8581 attgattcat ttatatcaaa aaaagagctt gaaaactaat caaactggac gtactttctt
     8641 cactgattta agatctggag ataaagttgc tgaagaaact tttgaaattt ttgtagaaaa
     8701 tctaacaaaa ggtttattaa cgatttctta tctacttaat ccagaaattc tcatattagg
     8761 aggtgggatt ctggatagta aggatatttt gttacctgaa attcaaagtt ctttagctaa
     8821 aaatgcaatg gataataggt ttttacctaa aaatcttgtg gcagctacat taggaaatga
     8881 agctggtcgt ataggagctg taaaaaattt cttagataga atttctaata aatagtatgt
     8941 aagataagga ggtgtcacaa tgactaactc tgtattttcg acaatgcaag atattg
//
LOCUS       DAYYQN010000071         8903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYYQN010000071 DAYYQN010000000
VERSION     DAYYQN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.072"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(261..1109)
                     /gene="dprA"
                     /locus_tag="ACHBRQ_001790"
     CDS             complement(261..1109)
                     /gene="dprA"
                     /locus_tag="ACHBRQ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9593406.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1205..1894)
                     /locus_tag="ACHBRQ_001791"
     CDS             complement(1205..1894)
                     /locus_tag="ACHBRQ_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFM9593407.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1906..2784)
                     /locus_tag="ACHBRQ_001792"
     CDS             complement(1906..2784)
                     /locus_tag="ACHBRQ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFM9593408.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2774..3643)
                     /gene="licA"
                     /locus_tag="ACHBRQ_001793"
     CDS             complement(2774..3643)
                     /gene="licA"
                     /locus_tag="ACHBRQ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HFM9593409.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3660..4682)
                     /locus_tag="ACHBRQ_001794"
     CDS             complement(3660..4682)
                     /locus_tag="ACHBRQ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HFM9593410.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4687..5394)
                     /locus_tag="ACHBRQ_001795"
     CDS             complement(4687..5394)
                     /locus_tag="ACHBRQ_001795"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFM9593411.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5730..7217
                     /gene="tacF"
                     /locus_tag="ACHBRQ_001796"
     CDS             5730..7217
                     /gene="tacF"
                     /locus_tag="ACHBRQ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HFM9593412.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7227..8030
                     /locus_tag="ACHBRQ_001797"
     CDS             7227..8030
                     /locus_tag="ACHBRQ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HFM9593413.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKI
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8032..8841
                     /locus_tag="ACHBRQ_001798"
     CDS             8032..8841
                     /locus_tag="ACHBRQ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HFM9593414.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2792 a         1791 c         1506 g         2814 t
ORIGIN      
        1 agcactgctt tgaggttgta gataagactg acgaagtcag ctcaaaatac tgttttgagg
       61 ttgcagatgg aagctgacgt agtttgaaga gattttcgaa gagtattata tgagaaaaat
      121 cctctactct actaatccac ttagaaaaaa cgcatattat caagtgttaa aaacttgcta
      181 atatgcgttt tattatggaa atgttgggtt cattttctct taaaattgat tttttcttag
      241 aattttagct taggtcattt ttaaaattca aattccgcaa gaacatcttg cccactggtg
      301 accaattttg ctccttcttg aatcaaatga tggcaaccgt ctgatagtcc atctaaaatg
      361 ctaccaggaa tagcaaagac atcgcgtcct tcttccattg ctcgctcaca cgtaatgaga
      421 ctacctgaac gcatcttagc ctctgctaca atcacaccac gacaaagtcc agcaatgatg
      481 cgattacggg caggaaaatg aaatttcaga ggttgttcgc caggtccata ttcacttaga
      541 accagatgat cattgccgat gtagtcttgc aagcgtttat tggctttagg ataaaacaca
      601 tccagtcctg ttccaatcac tgcaatggtt tttccgccat tctgaagagc tgccatatga
      661 gctgctgtgt caatgccctt ggccagacca ctgacaataa ccagttcatt ttccaagcct
      721 tgaatgactt tttcaactga cttagctccc tgtttgctac aagcacgact gcccacgacc
      781 gctaccttcg ggaatttcag gaggtcaaga tttcccttgt aaaataaaag tacaggcgca
      841 tcatatattt cactcaaatc ccaaggataa cagtcatcta aaatagagaa agatggaaat
      901 ttttgaaact ctttcgacaa atgcgcatcg tctatctgaa aataacgttc cataaaaacg
      961 gctggattac ggcaacctga tatatctgca atatcaccca acaaaagctc ctgatcaaca
     1021 ttttcaccgt attctagcac tttcaaaatc tgttgattgg tcaaacctga tttttttaac
     1081 ttatagattt catagtttgt gattttcata aataactcca tttctttttc tactcatcta
     1141 tctattcgta aaagttcgta aaaaatcaaa aaacatccga ctattttgtc agatgttgga
     1201 atctttaatt ttcgttttta agaatttctt ctaatttacg atagtcttgg acactatcga
     1261 tctcataaat gctattgcct tctaattctt caacatagac atctagctct ttgatattat
     1321 ccttaaccat attgtcccaa tagagatcaa caaattcacc acttgcataa gccttgtcga
     1381 taaagctgac aatcttttct gcagttggag catcccagaa ggatacacca ctaaggatgc
     1441 gacctgcctt gctatcaaca ataatgtctt gaaccttgta gtcatctcca tagaccaaga
     1501 accattcgtt ggtacaatct tcacgataaa cactaaaata agtcgaacgt gtcaaatcat
     1561 tgcggaacat atttttaaag agataattgt cagcatcaat aacatagctg ttggccaatt
     1621 cttcttttac aagatagaga gagtaaaagt tattgtagtc agcgtattta tcattgaaaa
     1681 cgaggcgaac accgtatttt tcttttaagt aatcgaattg ttctttaaga taaccaacaa
     1741 tgatgatgat gtcattgatt cctttttctt tgagaaactc aatttggtat tcaatcaaag
     1801 gtttttgatt aacctgaacc aaggctttag gggtattttc agtcatagga cgcaagcgag
     1861 ttcccaatcc cgctgctaag ataatggctt tcacacgaat ctcctttatt ctttaataat
     1921 aatataaact ccagcaatga cgacaagtga cgtcataatg gtcagcatat ctagcggtgc
     1981 acccaaaaaa acaactgcaa acaagaccgt ccatactaca tagctcacgt tcaagcctgt
     2041 agccttggct ggttgcaagc gattgatagc gatataataa gccaagtagg aaatcatatc
     2101 aaaggctgca aaaacaatca tgagacctag caattgtcca ttggctactg cagtaaatga
     2161 ctgatgagag aagagcacaa tcacaagata ggacaagaac gaagtcactt gacggattaa
     2221 gagggcttcg atttcactca gttcactttc catagcaaag gagctaagaa cactctcact
     2281 tccccatgca atagcacaaa ccaaagcaca gagaatccca atgtagaagg aattaacctg
     2341 ttcaacctta taggtctggg caataatccc tccaataatc aagacaatcc caaatacagt
     2401 atttttcgaa atcttgtgct tcaaaaagaa aaaagccaat agaactgaaa tcgcagggta
     2461 aatagccgat acagatgaag ctaaagaact tccgatatac ttaactgcat aaagattggc
     2521 ctgcataccg atagggcctg ctagcaaggc tccgatgata acactgacat tgcgaatatt
     2581 taagaaaatt gagaggcgaa ctttcccttc ttttaccaag agaaaagcta gtaagataaa
     2641 gatgctcaaa aaatcatgag ctgcagccac cacaaagggt gacaaatctg taaaaatcga
     2701 aaagatataa gcactaaccg ttagacccaa gccccagaaa atacctgaga gaaggccaaa
     2761 aggaactccg tttttacttt tcatctgaac ctccataaga agccaaacct ttaatagctc
     2821 tttggtaacg attcacacca tagtcaccaa aatcttcacc ttgctcttcc ttatagacag
     2881 tccatagact ccaaatagta tcttgtaaaa ttttataaat agcaatcttt tcatgagaaa
     2941 ccggtgtttg gtcactctca tagtgagata agaaagtttc ttcctcttgg gaagtgaatt
     3001 cagactctaa aaagagggca gccaaatccc acattggatc attcattgat gaatattccc
     3061 agtcaatcaa ataaagtcgt ccttgaggtg attcgataaa gttttcaggc accaaatcga
     3121 tatgacaaga ttttctgtca acacctaagt cagccagtct tttctctaag gagaagactg
     3181 catttctaac agattcatag ttggcataag gaatttgttc ttcaatcaag gattcgtatt
     3241 ttttgatttc ttcaaaagga gcaaattctc ctcttaattc cttagcagac gtatgaatag
     3301 tttgtaatat tggagtaatt ttgtcgaact tggtcttgat tgacgttgaa tcaagcgtaa
     3361 tcgcagattc gatatactca tttactttga taccagcttc aatatcaaaa agataatttt
     3421 ttacatctaa gcctaaatcc tttagtagtt caagattgta cttttcatct tgtcgattga
     3481 taagcttttc tgtcccttta ccaaagaatt taacaatgta ttgcttattt gttgttttgg
     3541 ccaaatagtt ttgattggtc attccaccca gttgttcaac actgaggact tcctcttctt
     3601 gactaagtaa ggaagaaatt ttttctttaa tgattttctc cacaattaac ctccagtact
     3661 tatacttccc acttaaacac tgttttaaag gctgtgttta aatcggttgc aaagacacga
     3721 tgaatatctt taatttctct tacaggttct tctagataaa ggatattttt aagacgatta
     3781 gcaaatttct tgacttccat catttggata gcattttcaa aatcaatgcg accagaacga
     3841 gatgacccaa ccaaaatcaa gcccttttct aaggcatcgc gagtattgag attgacttta
     3901 tattcgctaa ctcccatcat gagaatcgtt ccctgaggac gaatgtagcg aatcaagtca
     3961 ttaatagctg gtccagtacc atcaccacca caacattcaa aagcatggtc aaaggccaaa
     4021 tcttcaggaa tattatccgt aatatagcat tctttggcaa atgagaagag ttccaacttt
     4081 tcccaatgac gaccaataac cacaatctct gcttctggca aagtatagtt gataatattg
     4141 gcaaccacaa aagctaaact tccatctcca ataacggcga tccgctcccg cttgctatga
     4201 gcaagagtca atagacgatt catagcgtgc atgcccacac tgacaaactc tgtaatggct
     4261 gcaaccgtat cttcaatagc atcataagcc accacacgat ctttagggag agaaacaaac
     4321 tctctcataa agccatcaaa tccactagac aagaaatggg tccctgtcat gtagttttca
     4381 tagaattctt catcactctg cataggagac tgattgggaa tcatgacaac tttttgacca
     4441 acctcgtagg ttccggtcgg gtcagaaatg acggttccac atgactcgtg aatcattgcc
     4501 attggaagct ttttattcaa aatcttggga tcacgttttc cctgatagta acgctgatcc
     4561 gcatgacaga cagccatgta gttgggacgg ataaggatat gattctcttg gtcaatagcc
     4621 tcttcctgat atttgacatt gataaactta ggcttagtta gttgataaat ttgattaatc
     4681 attttactag tctttctcaa tcatactttt tgcaatcttc aaatctgtta cggttgtaat
     4741 cttcagattt gagtattcac ctttggccaa agccacatct tttcctttga tcacaaagat
     4801 tttacatgca tctgtcaaga tttccttctc ttcatcagaa agagatccat aaaggtccat
     4861 gaagtccttg caacggaatg tttgaggtgt ttgtccttga taaaggtgag cacgatttgg
     4921 aatatctgta ataaattgac cattggtact ttcaacgata gtatcaaccg cttctaccac
     4981 tgtgtccact gcgtcatgat tttgggcaag ttggatattg tcctgaatca tgcgaagtgt
     5041 aataaatgga cgaacagaat cgtgggtaac aacgatatcc tctggagtaa gcggacgata
     5101 agcatcaatg gcttcaatga tgttcttaat acttgtattg cggtcagcac caccctttgt
     5161 aatgatgata cgttccttat aaagaggaag atatttatct acaagatctt ctgcatgaga
     5221 aacccagtct ccatgaacac caactacaat tttttcaata cttggctcca agacaaattt
     5281 ttcaattgta tgaatcaaaa taggtcgatc acctagctct aaaaattgtt ttggcaagtt
     5341 actgatcccc atgcgtgtgc cagttccacc ggcaagaatt cctgcataaa tcatctattt
     5401 tctcctttgt cttactaaaa aaacttattc tctctattat accacaatct agtcctatca
     5461 taaaagaaat actggtcctc cccttctaga ataggagaat tatgcagttc tattattcaa
     5521 agaaactagt ccatttctag taatgactag gaattctgta gcaattacta ataaatggct
     5581 gcgaaatttt agagtccttc agaataatat actttcctta aagaaaactt aaaacttaag
     5641 atttgaaact tcaaagtgat tacttgactt actgcccttc atttcttatt agctaacttt
     5701 atgatataat gaaaaacgag gtaaattgaa tgaaaagtat aaaattaaat gctctatctt
     5761 acatgggaat tcgtgtcttg aatattattt ttcccatcct aactggaacc tatgtcgcgc
     5821 gtgtcttgga ccgaactgac tatggttact tcaactcagt cgacactatt ttgtcatttt
     5881 tcttgccctt tgcaacttat ggtgtctata actacggttt aagggctatc agtaatgtca
     5941 aggataacaa aaaagatctt aacagaacct tttctagtct tttttatttg tgcatcgctt
     6001 gtacgatttt gaccactgct gtctatatcc tagcctatcc tctcttcttt actgataatc
     6061 caatcatcaa aaaggtctac cttgttatgg ggattcaact cattgcccag attttttcaa
     6121 tcgaatgggt caatgaagct ctggaaaatt acagttttct cttttacaaa actgccttca
     6181 tccgtatcct gatgctggtc tctattttct tatttgttaa aaatgaacac gatattgttg
     6241 tctatacact tgtgatgagt ttatcgacgc tgattaacta cctgattagt tatttttgga
     6301 ttaaaagaga catcaaactt gttaagattc acctaagtga ttttaaacca ctctttctcc
     6361 ctctgacagc catgttagtc tttgccaatg ccaatatgct cttcactttt ttagatcgcc
     6421 tcttcctcgt taaaacaggg attgatgtca acgttagtta ctataccata gctcagcgaa
     6481 ttgtgaccgt tatagctggg gttgtaacag gtgcaattgg agtgagtgtg cctcgtctca
     6541 gttactatct ggggaaagga gacaaagaag cctatgtttc tctggttaat agaggtagtc
     6601 gaatctttaa cttctttatc attccactga gttttggact catggtttta ggaccaaatg
     6661 ccatcctact ttacggtagt gaaaaatata tcggaggcgg catcttgacc tctctcttcg
     6721 cttttcgtac gattatcctg gccttagata ccattcttgg ttcccaaatt ctctttacca
     6781 atggctatga aaaacgtatc acagtttata cagtctttgc tgggctactc aatttgggct
     6841 tgaatagtct cctctttttc aaccgtatcg tggctcccga atactactta ctgacaacta
     6901 tgttatcaga ggcttctcta cttgttttct atatcatttt catccataga aaacaactca
     6961 tccacttggg acatatcttt agctatactg ttcgatactc tctcttttca ctttcctttg
     7021 tagcaattta tttcctgatt aatttcgtgt atcctgtgga tatggtcatt aatttgccat
     7081 ttttgattaa tactggtttg attatcttgc tatcagctat ctcttatatt agtctacttg
     7141 tcttcacaaa agatagcatt ttctatgaat ttttaaacca tgttctagcc ttaaaaaata
     7201 aatttaaaaa atcataggag tttaaaatga aacaactaac cgttgaagat gccaaacaaa
     7261 ttgaattaga aattttggat tatattgata ctctctgtaa aaagcacaat atcaactata
     7321 ttattaacta cggtactctg attggggcgg ttcgacatga gggctttatc ccttgggacg
     7381 acgatattga tttatccatg cctagagaag actaccaacg atttattaac atttttcaaa
     7441 aggaaaaaag caagtatgaa ctcctatcct tagaaactga taagaactac tttaacaact
     7501 ttatcaagat aaccgacagt acgactaaaa ttattgatac tcgaaataca aaaacctatg
     7561 agtctggtat ctttatcgat attttcccta tggatcgctt tgatgatcct aaggtcattg
     7621 atacttgtta taaactggaa agcttcaaac tgctgtcttt cagtaaacat aaaaatattg
     7681 tctataagga tagcctttta aaagattgga tacgaacagc cttttggttg ctccttcgac
     7741 cagtttctcc tcgttatttt gcaaataaaa tcgagaaaga aattcaaaaa tatagtcgtg
     7801 aaaatgggca gtatatggct tttatccctt caaaatttaa ggaaaaggaa gtcttcccaa
     7861 gtggtacctt tgataaaata atcgatttac cctttgagaa tttaagcctt cctgcacctg
     7921 aaaaatttga tactattttg acacaatttt atggagatta tatgacccta ccaccagaag
     7981 aaaaacgctt ctacagtcat gaatttcacg cttataaatt ggaggattag gatgcaatat
     8041 ttagaaaaaa aagaaattaa agaaattcaa ctagccctgc tggactatat tgatgagact
     8101 tgtaagaaac atgatattcc ttattttctc agttatggaa ccatgcttgg agccatccgc
     8161 cacaaaggta tgattccttg ggatgatgat attgatattt ccctttatcg tgaggattat
     8221 gagcgtttac tgaagattat tgaagaagaa aatcaccctc gctacaaggt tctttcctac
     8281 gatacatctt cttggtactt ccataatttc gcatcgattt tggacacttc tactgttata
     8341 gaagaccatg ttaagtacaa gcgtcatgat accagccttt tcatcgatgt cttcccaatt
     8401 gatcgattta cagacttgag cattgtcgac aagagctata agtatgtggc tcttcgtcaa
     8461 ctagcttata tcaaaaaatc acgagcagtt cacggtgata gcaaactaaa agattttctt
     8521 agattatgta gctggtacgc tctccgattt gtcaatcctc gctactttta caagaaaatt
     8581 gatcaactag tcaaaaatgc tgtaaccaac attcctcaat atgaaggagg agttgggatc
     8641 ggtaaggaag ggatgaaaga aatcttccca gttgatacct ttaaagaact gattttaact
     8701 gagtttgagg gccgtatgtt gcctgttccc aaaaaatatg accaattttt aacccagatg
     8761 tatggcgatt atatgacacc accatcaaaa gaaatgcaag agtggtatag tcatagcatt
     8821 aaagcttatc gcaaaaactg attgaggggg attatacaaa ctactaagat agaggttatt
     8881 caaaaacata attttagtag aaa
//
LOCUS       DAYYQN010000072         8655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYYQN010000072 DAYYQN010000000
VERSION     DAYYQN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.073"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            420..1421
                     /locus_tag="ACHBRQ_001799"
     CDS             420..1421
                     /locus_tag="ACHBRQ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9593415.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            1571..2488
                     /locus_tag="ACHBRQ_001800"
     CDS             1571..2488
                     /locus_tag="ACHBRQ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593416.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKVDFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            2499..3389
                     /locus_tag="ACHBRQ_001801"
     CDS             2499..3389
                     /locus_tag="ACHBRQ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9593417.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            3418..5034
                     /locus_tag="ACHBRQ_001802"
     CDS             3418..5034
                     /locus_tag="ACHBRQ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9593418.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            5044..6363
                     /locus_tag="ACHBRQ_001803"
     CDS             5044..6363
                     /locus_tag="ACHBRQ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9593419.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(6574..6646)
                     /locus_tag="ACHBRQ_001804"
     tRNA            complement(6574..6646)
                     /locus_tag="ACHBRQ_001804"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6611..6613),aa:Lys,seq:ctt)
     gene            complement(6911..8182)
                     /locus_tag="ACHBRQ_001805"
     CDS             complement(6911..8182)
                     /locus_tag="ACHBRQ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9593420.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            8474..>8655
                     /locus_tag="ACHBRQ_001806"
     CDS             8474..>8655
                     /locus_tag="ACHBRQ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593421.1"
                     /translation="MEMMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEI
                     LDLYENIKSKLDFAYQ"
BASE COUNT         2789 a         1479 c         1792 g         2595 t
ORIGIN      
        1 acacggtctc actaatatat ttgttaaatg ccatgatgtt tattcgatta gatgagtaac
       61 caaaaataac aaaaaacgct atttcgcgtt tgaactgtac ctcaaaagtt agacagaaaa
      121 agtctaattt ttggggtgtt tttgttaaga aaagaccttc aatcatttaa gcaaaatgta
      181 catctagatt tatagccatc taaaactctt gctttttttg tagaagatac gaaaaaagcc
      241 atgaaaccaa ggcttttcct gttttatgta gatgtttaca catgaaaatt gaagtatttt
      301 caaaagctaa ttggagtgat tttattcatg atgggataaa tgatctggcc aaaaatgatt
      361 gagaaatagt tgcttaaaaa gcatttgcat gatactatat attgaaagga ggtgataata
      421 tggtagaaca aagaaaatca attaccatga aagatgttgc tttagaagca ggagttagtg
      481 ttggaactgt ttcacgtgta attaataaag aaaaaggcat taaagaagta actttgaaaa
      541 aagtggaaca agcgattaaa actttgaatt acattccaga ttactacgct agaggaatga
      601 aaaaaaatcg aacagaaacg attgcaatca ttgtaccaag tatctggcat cccttctttt
      661 cagaatttgc tatgcatgtg gaaaatgaag tctataagag aaataacaaa ttactcttat
      721 gttctataaa tggtacaaat agagagcaag actatctgga gatgttgcgt cataataaag
      781 ttgatggagt ggttgccatt acctatagtc caattgaaca ttacttgacg tcaggaattc
      841 cctttgttag tattgaccgc acatactcag atattgccat tccttgtgtt tcatcagata
      901 atgatgcagg tggaagagaa gcagctaaac aattaattag taaaggatgt cagcatttag
      961 cctttgtggg cggacataat acaaccatta atgaaaccaa acgtcgtaga atttcatttg
     1021 aaaagtatgt ccaagagcat aaaatacctc ttagtatttt tgatttggat gagacagtta
     1081 ctgactatta tggaaagcta gagagtttct tggaagaaaa ttcttctata gatggaattt
     1141 ttacaatcaa cgattttaca gctttggatg tgattgaaat actagaaaaa aatggcaaac
     1201 gtattcctca tgatgtccaa attattggat acgatggaat taaaatggct ggagatagag
     1261 attatctact ttcaactatt gaacaaccat tggaagaaat ggcaaaagaa gcggttcgta
     1321 ttttgtttga tataattgat ggaaagactg ttaatttgca gacaatccta ccagtaaaat
     1381 ttgttgaagg aaaaacaaca aaaaatgaaa ataagtcttg acagaaagcg ttatcaatga
     1441 tagaatgaat tcagataaaa agatttattt ttaaaacaaa aatgaaacgt ttcaaaaaaa
     1501 gaaataaaaa gacagcgcca agcgctatct tttctagaaa aaatgaaacg tttcaaaaaa
     1561 ggaggttgct atgaatagca aagcgaagca agtttctctt tgggaaagaa tcaagaaaca
     1621 aaaactcttg ttattgatga ctgtccccgg tttagtttta acctttatct ttaaatacat
     1681 ccctatgtat ggggttttaa tcgcatttaa agattacaat cctttaaaag gaattttagg
     1741 gagtgattgg attggttttt ctgagtttac aaaattcata tcctctccca actttggtat
     1801 cttgttagcc aacacattaa aattaagtat ctatggttta ttgcttggct ttttaccacc
     1861 aatcattctt gcgattatgc tcaatcaact cttgagtgaa aaagtcaaaa aacgaattca
     1921 gctcatttta tacgcaccaa actttatctc agtcgttgtt attgtcggta tgattttcct
     1981 cttcttttca gtgggaggac caatcaacaa ttttctttct atgtttggaa tgaaggttga
     2041 cttcttgaca aatccagact tctttagacc tttatacatc tttagtggta tctggcaagg
     2101 aatgggctgg gcttcaacgc tctacacggc aacattggta aatgtagatc cagccttagt
     2161 agaagcagcc cgactggatg gagccaatat cttccaacga atctggcaca ttgatattcc
     2221 agctcttaag cctattatgg ttatccaatt tgttttagct gcaggtggaa ttatgaatgt
     2281 cggatatgaa aaagcattct tgatgcagac atcgttaaat ttgccaactt ctgaaattat
     2341 ctcgacatat gtctataaag ttggtcttgt atcaggagac tattcttact caacagcggt
     2401 tggtttgttt aatgcagtga ttaacgtagt attgcttgtt gcagttaacc aaatcgttaa
     2461 acgcatgaat aatggtgaag gaatttaagg aggaaagtat gaaaaattcg attatggata
     2521 caaaatttga tagacgtatc ttactcttaa ataaaatcat tattgtcttt atcgttttga
     2581 tgactttgct tcctttactt tatatcgtcg tagcatcctt tatggatcct aaggttctgg
     2641 ttagtagagg gattagcttt aatccagccg attggactgt agaaggttac cagcgtgtat
     2701 tcagtgacca atctattcta agaggtttta tcaattctct actatactct tttggatttg
     2761 cagctttaac agtattgcta tctgtgttta cagcttatcc tctttctaag aaagacttgg
     2821 ttggacgtcg ttggattaac tacttcttga ttgtgactat gttctttggt ggtggtttag
     2881 tcccaactta cttgctcgta aaagaattgg gaatgctcaa tactccatgg gctatcattg
     2941 ttccaggtgc tgtcaacgtt tggaatatta ttcttgctag ggcctatttc caaggattgc
     3001 ctgaagaatt agttgaagct gctgtcattg atggtgcaaa tgatttacag attttcttca
     3061 aaatcatgct tcctcttgca aaaccaatta tgtttgttct cttcctttat gcttttgtag
     3121 gacagtggaa ctcatacttt gatgcaatga tttatatcaa ggatccaaac ttggaaccat
     3181 tgcaacttgt acttcgtaaa attctcattc agagccaacc aggtcaagac atgattggag
     3241 cacaagcggc tatgaatgaa atgaaacgtt tagctgaatt gattaaatac gcaactattg
     3301 tcatttccag cttgccattg attgttatgt atccattctt ccaaaaatac tttgataaag
     3361 gaattatggc tggttcactt aaaggataaa aaaagaaaaa ataaaaggag ttttctcatg
     3421 aaattcaaaa cattctcaaa atcagcagtt ttgttgacag ctagtttagc agtacttgca
     3481 gcctgtggct caaaaaatac agcttcaagt ccagattata agttggaagg tgtaacattc
     3541 ccgcttcaag aaaagaaaac attgaagttt atgacagcca gttcaccgtt atctcctaaa
     3601 gacccaaatg aaaagttaat tttgcaacgt ttggagaagg aaactggcgt tcatattgac
     3661 tggaccaact accaatccga ctttgcagaa aaacgtaact tggatatttc tagtggtgat
     3721 ttaccagatg ctatccacaa cgacggagct tcagatgtgg acttgatgaa ctgggctaaa
     3781 aaaggtgtta ttattccagt tgaagatttg attgataaat acatgccaaa tcttaagaaa
     3841 attttggatg agaaaccaga gtacaaggcc ttgatgacag cacctgatgg gcacatttac
     3901 tcattcccat ggattgaaga gcttggagat ggtaaagagt ctattcacag cgtcaacgat
     3961 atggcttgga ttaacaaaga ttggcttaag aaacttggtc ttgaaatgcc aaaaactact
     4021 gatgatttga ttaaagtcct agaagctttc aaaaacgggg atccaaatgg aaatggagag
     4081 gctgatgaaa ttccattttc atttattagt ggtaacggaa acgaagattt taaattccta
     4141 tttgctgcat ttggtatagg ggataacgat gatcatttag tagtaggaaa tgatggcaaa
     4201 gttgacttca cagcagataa cgataactat aaagaaggtg tcaaatttat ccgtcaattg
     4261 caagaaaaag gcttgattga taaagaagct ttcgaacatg attggaatag ttacattgct
     4321 aaaggtcatg atcagaaatt tggtgtttac tttacatggg ataagaataa tgttactgga
     4381 agtaacgaaa gttatgatgt tttaccagta cttgctggac caagtggtca aaaacacgta
     4441 gctcgtacaa acggtatggg atttgcacgt gacaagatgg ttattaccag tgtaaacaaa
     4501 aacctagaat tgacagctaa atggattgat gcacaatacg ctccactcca atctgtgcaa
     4561 aataactggg gaacttacgg agatgacaaa caacaaaaca tctttgaatt ggatcaagcg
     4621 tcaaatagtc taaaacactt accactaaac ggaactgcac cagcagaact tcgtcaaaag
     4681 actgaagtag gaggaccact agctatccta gattcatact atggtaaagt aacaaccatg
     4741 cctgatgatg ccaaatggcg tttggatctt atcaaagaat attatgttcc ttacatgagc
     4801 aatgacaata actatccaag agtctttatg acacaggaag atttggacaa gattgcccat
     4861 atcgaggcag atatgaatga ctatatctac cgtaaacgtg ctgaatggat tgtaaatggc
     4921 aatattgata ctgagtggga tgattacaag aaagaacttg aaaaatacgg actttctgat
     4981 tacctcgcta ttaaacaaaa atactacgac caataccaag caaacaaaaa ctagaggttg
     5041 attatgggag ataagaaata cacagtagaa aaagccaatc gttttatagc agaaaataaa
     5101 catctcgtta atactcaata taagcctgaa gaacattttt cagctgagat tggttggatc
     5161 aatgatccaa atggatttgt ctattttcgt ggagaatacc atctctttta tcaattctat
     5221 ccatatgata gtgtttgggg gcctatgcac tggggacatg ctaaaagtaa ggacttggtg
     5281 acttgggagc acttgccagt ggcacttgct cctgaccaag attatgaccg aaatggttgt
     5341 ttctcaggct ctgccattgt caaggatgat cgcctctggc tcatgtacac tgggcatatc
     5401 gaagaaaaaa ccggtgtccg ccaagtgcaa aatatggcat tttcagatga cgggattcac
     5461 tttgaaaaga tttcccaaaa tccagttgta actggaacag acttgccaga tgagttgatt
     5521 gctgctgatt tccgtgaccc aaaactcttt gaaaaagatg gacgctatta ctccgtagta
     5581 gctgccaaac acaaggataa tgtgggctgt atcgttctac tagggtccga taacctagta
     5641 gaatggcagt tcgaatccat ctttttaaaa gggggagaac accaaggctt tatgtgggaa
     5701 tgcccagatt acttcgagtt agatgggaaa gattgcctta ttatgtcacc catgcgttat
     5761 cagcgtgagg gagactcata tcataacatc aactcatcgc ttttgttcac gggtaaggta
     5821 gattggggag aaaaacgttt tatcccagaa tcagttcaag aaattgatca tggccaagac
     5881 ttctatgcgc ctcaaacatt gttggacaat caaaatcgtc gtatcctgat tgcttggatg
     5941 cagacatggg ggcgtaccct tccaacccat gaccaagaac acaaatgggc atgtgccatg
     6001 actttaccta gaattctaag attggaagat ggcaaactaa gacaattccc tgttaaaaaa
     6061 ggccaatatc aaatccaaat agataaagat tgtcattacc acttaggaaa tgatatagat
     6121 tatcttgaat ttggttatga cagtaatgcg cagcaagttt acattgatcg tagccatctt
     6181 attcaaaaaa ttctaggtga agaagaacag gacactagtc gacggtatgt agatattgaa
     6241 gctaaagaat tggaagttgt tctagataaa aattccatcg agatttttgt caatcaaggt
     6301 gaagcaagct tgactgcaac ttattactta acggtgccag ctgagctatc acgaattgat
     6361 taaaaattaa gttatttctc ctaaagaaaa agttctcttt ctaaaatagt ggaaagagga
     6421 ctttttgtgt tttgggtata taagcttagt ttatggtatt tgtaaaattg gtgttggatt
     6481 atgatttaaa ctagttttct aaagaatttg aaaaaaattt tatttaagca aaaaaaagcc
     6541 ctatattata agacttttcc tgttgtattt aaatgccccc tacagggatc gaacctgtga
     6601 cccacggatt aagagtccgc tgctctgcca gctgagctaa ggaggcaaag aaaaaagctg
     6661 tattggtgcc gaaacttcac ggtttgtatt gaacccgcgc aattaagcag gtgggcaact
     6721 cgctctaact gaagctgttt ccgtgtgaga cggcctacat gctgttagaa gacttttgtt
     6781 tccctaataa tacaaaaaat agtcggtcaa cacttaagtg tgaagtcgta caccacagcg
     6841 tttctatatt tatatgatac cactttttca aaaaaaatca agaggaaagt gcaatttttt
     6901 gaaaaggatt tcagattttt cgcaaatggt cgatagcttc ctttctttga gccaaagccc
     6961 gttcatattt accagtatct tctgcttgga aatagtgatg attacgaatt ttttctggca
     7021 gatagtcttg ctttacccaa tttccaggat agttgtgtgg atagagatag tcttgggcat
     7081 tccccagttc cttgcttcca ctgtagtgcc catcacgtag gtgtcgcgga ataggcaagt
     7141 gccctgatgt tttgaggtca gcaagtgcct tatccatagc tacataggct gagttggatt
     7201 ttggagaaag ggccaaatca atcacgacat tggcaatgag aatgcgggct tctgggaacc
     7261 caatcttctg ggcagcatcc agagcagtca cggtatgaat ctgggcctca ggattggcta
     7321 agccgatatc ttcataggcg ataacagtca agcgacgagc gagactaggc agatccccag
     7381 cctcaatcaa gcgggcagta tagtggagac tggcatccac atctgagcca cggatagact
     7441 tttgcagggc agatagaaca tcatagtgac cgtctccatc cttgtccata gtgatgtagc
     7501 ttctctgaag actattttcc atgatgtcta aggtgatgtg gcgaatgccc tcgtcattct
     7561 caggggtaga gagaacagcc aagtccagtg agttaaaggc agagcgaagg tctccgtttg
     7621 tagaggttgc gatgaaatcc agcgcatcct catctagttc tattggaaaa tcaaaaccac
     7681 gttcagggtt acttagagct atctgcaggg cctctttgac gtcttggtta gacagaggtt
     7741 ccaactcaaa aatttgaaca cggctacgaa tggcaggagt gacggaaaag aaaggatttt
     7801 cagttgttgc tccaatcatg atgaccagtc cactttccaa gagaggcaag aggaagtctt
     7861 gcttggtctt atctagtcga tgaatttcgt ctagcaatag gacgagacca ccagaaaatt
     7921 tagcttcttc cgagatttct tgcagtcgct ttttactatc aactgtcgca ttgaaagttc
     7981 gaaaggcata cttggtcgtt ccagcgatgg cagaggcaat actggttttg ccgattcccg
     8041 gagggccata tagaatcatg gaggacaggc ggttggcttc caccatgcgg cggatgattt
     8101 ttccaggtcc gaccagatgc tcctgaccga tgacctggtc gatggtttta gggcgcatgc
     8161 gaagcgcgag attgtctggc atagcagtcc tttctagcgt ggatttgagt agaatgactc
     8221 atctactata tcttactatg tccattctag cctattagtt gtgatatttc aatggttttg
     8281 taataatcag ggagtgagat agaatctttt ttactattta atttaatgag gagaggtatc
     8341 agcatgagag atttgatttt ccttaaagct caaaatagga tagttgagcc taaagaagtg
     8401 gaagtgctga cttgagaaat aatattgctg aatgtcattg tatgaactat aatgaaaaga
     8461 gaggtgataa gcaatggaaa tgatggaact accaagtcag gaaattttga tttttacaaa
     8521 acaaattcgt cattggattc ttagtgatca agttatttca gggaaaagaa aacttttctt
     8581 ccgggaagat actccaaaag agattttaga tttgtacgaa aacattaaat ctaaacttga
     8641 ttttgcttac caaga
//
LOCUS       DAYYQN010000073         8578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYYQN010000073 DAYYQN010000000
VERSION     DAYYQN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.074"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..387
                     /locus_tag="ACHBRQ_001807"
     CDS             <1..387
                     /locus_tag="ACHBRQ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM9593422.1"
                     /translation="FENYNVFVPEANGSGALGEVLSTPLIGEPLIGHTDTFLSIGNFK
                     TKFEADACIKFIKTKFARVLLGVLKVTQHNSRKTWYYVPLQDFTVNSDIDWTQSVTDI
                     DRQLDQKYDLSPEEIAFIENHVREMD"
     gene            552..3806
                     /locus_tag="ACHBRQ_001808"
     CDS             552..3806
                     /locus_tag="ACHBRQ_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9593423.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(4192..5178)
                     /locus_tag="ACHBRQ_001809"
     CDS             complement(4192..5178)
                     /locus_tag="ACHBRQ_001809"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9593424.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            5372..7840
                     /gene="gyrA"
                     /locus_tag="ACHBRQ_001810"
     CDS             5372..7840
                     /gene="gyrA"
                     /locus_tag="ACHBRQ_001810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9593425.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            7840..>8578
                     /gene="srtA"
                     /locus_tag="ACHBRQ_001811"
     CDS             7840..>8578
                     /gene="srtA"
                     /locus_tag="ACHBRQ_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HFM9593426.1"
                     /translation="MSQKNNKKKNKRKNLLTNILARFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQF"
BASE COUNT         2765 a         1478 c         1837 g         2498 t
ORIGIN      
        1 ttcgaaaatt ataatgtttt tgtaccagaa gctaatggaa gtggtgcctt aggtgaagtc
       61 ttatcaacac ccctaatcgg ggaaccccta atcgggcata cagatacttt tttatctatt
      121 ggtaatttta aaacaaaatt tgaagccgat gcttgtatta aatttattaa aactaaattc
      181 gctagagtat tattaggtgt tttgaaagtt actcagcata attcacgcaa aacttggtat
      241 tacgtccccc tccaagactt tacggtcaat tcggacattg attggacaca atcagtgact
      301 gatattgacc gccagcttga tcaaaaatat gacctttccc ctgaagaaat tgcctttatt
      361 gagaatcatg taagggagat ggattagaaa agtattttta tttgacaaat agtgctcaat
      421 gatctaaaat gactatatag gattaggtca ggaagcatac gatgccctga ccctttttgt
      481 acttatgaga tgagaaagtc atttgttaga taaattgact tcgttagcaa acgttcaaaa
      541 aaggaaaact tatgccagta gaaattaaaa ccactaaaga aattcatcct aaaatctatg
      601 cctacaccac accgacagta accagtaatg aaggctggat taagattggg tatacagaac
      661 gtgatgtcac acaacgtatc aaggagcaaa cgcatacagc tcatatagct acagatgtct
      721 tatggactgg tgatgcagct tatacagaag agcctgataa ggggaaaact ttcaaggacc
      781 atgatttcca ccatttcctt tctttccatg atgtagaacg tcgtcccaag acggaatggt
      841 tctattttaa tggaactcct gaaaaatcaa aaaatctttt tgataagttt gttcagcatg
      901 atttgtctgg ttatcagcct ggaaaaggac aggactatac tctgcgacaa gagcaagaag
      961 aagcagttgc taagacatta gcttatttcc aagaacatgc tggaggcaag tttctctgga
     1021 atgccaagcc acgctttggt aaaaccttgt ctacctatga cctagctcga cggatggaag
     1081 ctgtcaatgt cctaattgta acaaaccgcc ctgccattgc taactcatgg tatgatgatt
     1141 ttgaaacatt catagcaggt caaacgactt acaagtttgt ttctgaatca gatagcctta
     1201 agagtcgtcc aatcttgtca cgacaagaat ttcttggtat tttagctgac gatgtaagac
     1261 aacttgcttt tatcagtctc caagacttga aaggatctgt ttatttaggt ggagagcacg
     1321 ataaactcaa atgggtaact gatctgcatt gggacttgtt ggttattgac gaggctcatg
     1381 aaggagttga taccttcaag actgaccaag cctttaataa gattcgacga aattttactc
     1441 tgcatttgtc aggtacatca tttaaagcat tggctaaagg agattttaca gaggaacaaa
     1501 tctacaactg gtcttatgct gatgagcagg ctgctaagta ttcgtggtct cttgagcaag
     1561 aagaggaaaa tccttatgaa agcttgcctc agttgaatct ctttacctat caaatgtctc
     1621 agatgattgg cgaaaagtta gaaaaaggcg ctcagatcga tggtgaaaat attgactatg
     1681 tttttgactt aagtgaattt ttcgctacag atgataaagg gaaatttatt catgagcatg
     1741 atgtcagaaa ttggttagat actctatcaa gcaatgaaaa atatccattt tcaaccaaag
     1801 aactccgtaa tgaactcaag catacttttt ggcttttaga acgtgtcgct tcggccaaag
     1861 cattaaaagc cctactagaa gaacacccaa tctatgaaaa ctatgagatc gttctagctg
     1921 ctggtgacgg acgtatgtcc gaagaagacg ataaagtcaa actcaaatcc ttggacttgg
     1981 ttagaaaagc gatagcagag aatgacaaaa ccattaccct atccgttggt cagctgacga
     2041 caggtgtcac tatccctgaa tggacaggtg tattgatgtt atcaaatttg aaatcaccag
     2101 ctctttatat gcaggccgcc ttccgtgctc aaaatcctta ctcatggagc gataacaaag
     2161 gaaatcactt tcgcaaagaa agagcctatg tatttgactt tgcgccggaa agaaccttga
     2221 ttctctttga tgagtttgcc aacaacttat cgcttgtaac tgcagctggt agaggaactt
     2281 cagctacacg cgaagaaaat attagagaat tattaaactt ctttccaatt attgccgaag
     2341 accgtgctgg taagatggtt gaaattgatg caaaggcagt tctaaccact cctcgccaga
     2401 taaaagctag agaagttctt aaacgaggtt ttatgtccaa tctcttattt gataatatta
     2461 gtggtatttt ccaagcaagt caaacagttt tagatatttt aaatgagctg ccagttgaaa
     2521 aggaagggaa ggtacaagat agttctgatt tattagattt ttcagatgtt acagtcgatg
     2581 atgagggaaa tgcagtagta gaccatgaaa ttgtagttaa tcagcaaatg cgactttttg
     2641 gtgaaaaagt ttatggactt ggtgaatctg ttgctgagtt agtcacaaaa gatgaggaac
     2701 gaactcaaaa acagctggtc aatgacttga gtaagaccgt ttcttcagtg attgtagagg
     2761 aattgaaagc agattattct ctaaaaacaa gggaaactga gcaaattaag aaacaaatta
     2821 cagcaacact tgagaatgaa attcgaaaaa atgatatcga aagaaaaatt tctgaagctc
     2881 atatcaagca agagttgcaa cagcagctca aagaagcaaa tgataaagcg caaaaagata
     2941 agattcaaga agatttggaa aaacgtttag aagaaaataa actcattcat aaagaaaaac
     3001 tagaacaaac actcaaaaaa gaagtggaaa aaatgcctga gaaatttatc gaacaggttg
     3061 agataaaacg tgtggaacag ttgaaacaat cagctcaaga tgaaattcgt gaccatttac
     3121 gagggtttgc aagaacaatt ccaagtttta ttatggctta cggtgatcaa actctaacac
     3181 ttgataattt tgatgccttt gttcctgaac atgtttttta tgaagtaaca gggattacga
     3241 ttgatcagtt tagatatttg cgagatggtg ggcaggattt tgcagggcat ctctttgata
     3301 aagcaacatt tgacgaagct attcaagaat ttcttcgcaa gaaaaaggag ttggcggatt
     3361 attttaaaga tcaaaaagaa gacatttttg actatattcc accgcagaag accaaccaaa
     3421 ttttcactcc taaacgagtg gtgaaaagga tggtagatga tttggaaaag gaaaatccag
     3481 ggatttttga tgatccatct aagactttta ttgatttata tatgaagtca ggcctctata
     3541 ttgcagaact tgtgaagcgg ttatataata gcaatggctt gaaagaggcc tttccaaatc
     3601 ctgaagaacg cttaaaacat attttggaaa agcaagttta tggatttgct ccgtctgaga
     3661 ttatctataa catttccact aattttatat ttggtaatct ttctaaagat atcagtagga
     3721 agaattttgt tttagcagat accattccag cggctaaaga agggagcatt caaaagttgg
     3781 ttgattccta ttttgaaaat aattaaaaag aaggccgagt caaaattctt tgaaatcaga
     3841 aaaaacgcat aatattgagt gcttttgtac tgccccccaa aagttagaca gaaaaaatct
     3901 aacttttggg gggcagttca gacaatcctt ggtattatgc gttttattgt gggaagatgt
     3961 ataatggatt gaaataagat atgaacaaat cgattaggaa aggaaaattg atttatagaa
     4021 atgttttagt agtcgatgcg tactgttata gattcaacga actataaaat ctgaagaaag
     4081 caattttaac cagatcttag aagcagttgt gtactactct aacttcaata gactatatca
     4141 tatagataaa aaacaactcc ctgatgattc aaggagttgt ctatagttaa attagttttt
     4201 agaagcttct tggaattctg gatttttcca tgcttcgtca ataatagctt gcaattcttt
     4261 agcagatgct tgcatttttt gagtttctgc atcgttcaat ggaatgttta ctggacgaac
     4321 gataccatgt gcaccaacaa cagctggttg accgataaag acattctcaa ctccgtattg
     4381 accttcttgg aatactgaaa gtggaagtac tgcgttttca tcgtcaagga ttgctttagt
     4441 gatacgagca agggctactg cgataccgta gtatgttgca ccttttttgt tgatgattgt
     4501 gtaggctgca tcacgaacac cttcgaacaa ttcaatcaat tcagcttctt gaacattttg
     4561 agtgtcttta aggaattctt caaggtttac accagcgatg ttagcgtgtg accaaacagc
     4621 gaactcagag tcaccgtgtt cacccatgat gtaggcgtgc actgaacgag catccacatc
     4681 caatttttca gcaagtgctt gacggaaacg agctgagtca agtgaagtac ctgaaccgat
     4741 aacgcgttct ttagggaaac cagagaattt ccaagttgag taagtcaaaa cgtcaactgg
     4801 attagcagca acaaggaaga tacctttgaa accagattca acaacttgag ttacgattga
     4861 tttgttgata gcaaggtttt tacctacaag gtcaagacga gtttcacctg gtttttgagg
     4921 tgcacctgca gtgatcacaa caaggtcagc gtctgcacag tcagagtatt gagctgcata
     4981 gattttttta ggtgaagtga aggcaagggc gtgactaagg tcaagcgcat caccaacagc
     5041 tttttcatgc aattgtggaa tttcgataat tccaagctct tgtgcaattc cttggttaac
     5101 aagtgcaaaa gcgtaagatg aacctacagc accatcaccg acaaggataa cttttttgtg
     5161 ttgtttagtt gaagtcattg ttttaaacat ctccttaatt ttattagggg attttcccta
     5221 gacaacttca ttctatcact tttaaaaaac tttgtcacga atatgcctta tagttctcga
     5281 tgtaaacgtt ttagtggttt agaggctgaa atagatggga atttatggta taatgttgtt
     5341 acttactaat tgtgaaatga ggcatttatt aatgcaggat aaaaatttag tgaatgtcaa
     5401 tctgacaaag gagatgaagg caagttttat cgactacgcc atgagtgtta tcgtagcgcg
     5461 agctcttcct gatgttcgag atggcttaaa acctgttcac cgtcgcattc tctacggaat
     5521 gaatgaattg ggtgtgaccc cagacaaacc ccataaaaaa tctgctcgta ttacagggga
     5581 tgtcatgggt aaatatcacc cacacgggga ttcctctatt tatgaagcca tggtccgtat
     5641 ggctcaatgg tggagctacc gttacatgct tgtagatggt catgggaatt ttggttccat
     5701 ggatggagat agtgctgccg ctcaacgtta taccgaggca cgtatgagca agattgctct
     5761 ggaaatgctt cgtgatatca acaaaaatac agttgatttc gttgataact atgatgccaa
     5821 tgaacgggaa cccttggtct tgccagcgcg ttttccaaac cttttggtta atggagcaac
     5881 tggtatcgcg gttgggatgg caaccaatat tccacctcat aatctgggtg aaaccattga
     5941 tgcagtgaag ttggtcatgg ataatcctga agtgactacc aaggacttga tggaagtctt
     6001 gcctggacca gattttccaa ctggtgctct tgtcatgggg aaatcaggta tccataaggc
     6061 ttatgaaaca ggtaaaggtt cgattgtcct acgttctcgt acagagattg aaacgactaa
     6121 gactggtcgt gagcgcatcg ttgtaacaga atttccttac atggtcaata aaaccaaggt
     6181 gcatgagcat attgttcgct tggttcagga aaaacgcatt gagggtatca cagcagtacg
     6241 tgatgagtca aaccgtgaag gtgttcgatt tgttattgaa gtcaagcgcg acgcctcagc
     6301 caatgttatt ctcaataacc tcttcaaaat gacccaaatg caaaccaatt ttggtttcaa
     6361 tatgctcgct atccaaaatg gtataccgaa aattttgtct cttcgtcaga ttttggatgc
     6421 ttatatcgag caccaaaaag aagtggttgt tcgtcgtaca cgttttgata aggaaaaagc
     6481 ggaagcgcgc gctcatatct tagaaggtct cttgattgcg ctagaccata tcgacgaagt
     6541 gattcgtatc atccgtgcta gtgaaacgga tgcggaagct caagctgagt tgatgagcaa
     6601 gtttaagctt tctgaacgtc aaagtcaagc tatccttgat atgcgtcttc gtcgtttgac
     6661 aggtttggaa cgcgataaga ttcaatctga gtatgatgac ctcttggctc tgattgcgga
     6721 tttagcagat attcttgcta agcctgaacg tgtttctcaa attatcaaag acgaattgga
     6781 tgaagttaaa cgtaaatttt ctgataaacg ccgtacagag ttgatggttg gacagatttt
     6841 gagtctcgag gatgaggact tgattgaaga atcggatgtc ttgattaccc tttctaacag
     6901 aggctacatt aagcgtttgg atcaggacga gttcactgct caaaaacgtg ggggtcgtgg
     6961 tgtccaagga acgggagtga aagatgatga ctttgttcgt gagttagtgt caactagcac
     7021 acatgatcat ctgctcttct tcacaaacaa gggacgtgtc tatcgtctta aaggttatga
     7081 aattcctgag tatggtcgga ctgccaaagg gctaccagta gtcaatctct tgaaattgga
     7141 tgaagacgaa agtattcaga cggttatcaa tgttgagtct gatcgcagtg atgatgctta
     7201 tctcttcttt acaacccgtc acggtattgt gaagagaact agtgttaagg agtttgccaa
     7261 tattcgtcaa aatggtctca aagcgctgaa tttaaaggat gaagatgagt taatcaatgt
     7321 cttgttggca gaaggagata tggatattat cattggtacc aagtttggtt atgcagttcg
     7381 ctttaatcaa tcagccgttc gtggtatgag ccgtatcgcc actggtgtga aaggtgttaa
     7441 ccttcgtgaa ggagacacag ttgttggtgc cagcttgatt actgatcaag atgaagttct
     7501 tattatcaca gaaaaaggat atggtaagcg tacagtcgct actgaatacc caacaaaagg
     7561 tcgtggtggt aagggaatgc agacagctaa aattaccgaa aaaaatggct tgctggccgg
     7621 tcttatgact gttcaagggg atgaggattt gatgattatc actgatacag gtgtcatgat
     7681 tcgaaccaat cttgccaata tttcacaaac aggacgtgca actatgggag ttaaagtaat
     7741 gcgcctggat caagatgctc agatagtgac tttcacaacg gttgcggtgg cagaaaaaga
     7801 agaagttggg acagaaaacg aaacagaagg tgaagcataa tgtctcaaaa aaataataaa
     7861 aagaaaaaca agcgaaaaaa tctgctgaca aatatcctag caagatttct gatattactg
     7921 tcactggctt tgatttttaa tactcaaatt cgaaatattt tcatagtctg gaataccaat
     7981 aagtatcaag ttagccaggt atcaaaagaa aaattagaag aaaatcagga tacagaaggc
     8041 aattttgact ttgattctgt caaagctatc tcttcggaag ctgttctaac ttctcaatgg
     8101 gatgctcaaa aattaccagt tattggggga attgcaattc ctgaattgga aatgaatttg
     8161 ccgattttta aaggacttga taatgttaat ctcttctacg gagctggtac aatgaaacgc
     8221 gagcaagtaa tgggagaagg aaattatagt ctagctagtc accatatctt tggtgttgat
     8281 aatgctaata aaatgttatt ttctccttta gataatgcta aaaatggcat gaagatttat
     8341 ctaaccgata aaaataaagt ttatacttat gaaatacgtg aagtcaaacg tgtgacaccg
     8401 gatcgtgttg atgaagttga tgatagagat ggggtcaatg aaatcacatt agtaacctgt
     8461 gaagaccttg ctgctacaga acgtattatt gtcaaaggtg atttgaaaga aacaaaagat
     8521 tattcacaaa catctgatga aatcctaaca gctttcaatc aaccatataa acaatttt
//
LOCUS       DAYYQN010000074         8166 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYYQN010000074 DAYYQN010000000
VERSION     DAYYQN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.075"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..2304
                     /gene="mfd"
                     /locus_tag="ACHBRQ_001812"
     CDS             <1..2304
                     /gene="mfd"
                     /locus_tag="ACHBRQ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9593427.1"
                     /translation="QSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRH
                     GFHFVDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQ
                     YLGIETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHF
                     KKAKQKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRS
                     IEEIKRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHY
                     TNFKERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLG
                     LMIIDEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTN
                     RYPVQTYVLEKNDSVIHDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGY
                     VHGRMSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQ
                     LRGRVGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAG
                     NLLGKSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTY
                     ISDQRHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVF
                     VQRVERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKK
                     DYEILEGLLIFGESLLEIKEFKEENSI"
     gene            2362..2628
                     /locus_tag="ACHBRQ_001813"
     CDS             2362..2628
                     /locus_tag="ACHBRQ_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9593428.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            2621..2989
                     /locus_tag="ACHBRQ_001814"
     CDS             2621..2989
                     /locus_tag="ACHBRQ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9593429.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            2994..3116
                     /locus_tag="ACHBRQ_001815"
     CDS             2994..3116
                     /locus_tag="ACHBRQ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HFM9593430.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            3109..4377
                     /locus_tag="ACHBRQ_001816"
     CDS             3109..4377
                     /locus_tag="ACHBRQ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9593431.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            4374..5651
                     /gene="tilS"
                     /locus_tag="ACHBRQ_001817"
     CDS             4374..5651
                     /gene="tilS"
                     /locus_tag="ACHBRQ_001817"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9593432.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            5655..6197
                     /gene="hpt"
                     /locus_tag="ACHBRQ_001818"
     CDS             5655..6197
                     /gene="hpt"
                     /locus_tag="ACHBRQ_001818"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9593433.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            6213..>8166
                     /gene="ftsH"
                     /locus_tag="ACHBRQ_001819"
     CDS             6213..>8166
                     /gene="ftsH"
                     /locus_tag="ACHBRQ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9593434.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         2810 a         1211 c         1695 g         2450 t
ORIGIN      
        1 cagtctagca attcaatggg aagtaaaaca ttggaggata tgttagagga atatcagatt
       61 aaattggatt ctagagataa gacaaatatc tgtaaagaat ctgtaaactt aatagagggt
      121 aatctcagac atggttttca ttttgtagat gaaaagattt tattgataac tgaacatgag
      181 atttttcaaa agaaattaaa gcgtcgtttt cgaagacaac atgtttcaaa tgcagagaga
      241 ttaaaagatt acaatgaact tgaaaaaggg gactatgttg tccatcatat ccatgggatt
      301 ggtcaatatc taggaattga aaccattgaa atcaagggaa ttcatcgcga ttatgtcagt
      361 gtccaatacc aaaatggtga tcaaatttct atccccgtgg aacagattca tctactgtcc
      421 aaatatattt caagtgatgg taaagctcca aaactcaata aattaaatga cggtcatttt
      481 aaaaaggcca agcaaaaggt taagaaccag gtagaggata tagctgatga tttaatcaaa
      541 ctctactctg aacgtagtca gttgaagggt tttgctttct cagctgatga tgatgatcaa
      601 gatgcctttg atgatgcttt cccttatgtt gaaacggatg atcaacttcg tagtattgag
      661 gaaatcaaga gggatatgca ggcttctcag ccaatggatc gacttttagt tggggatgtt
      721 ggttttggaa agactgaagt tgctatgcgt gcagccttta aagcagtcaa tgatcacaaa
      781 caggttgtca ttctagttcc gacgacggtt ttagcgcaac agcactatac gaattttaag
      841 gaacgattcc aaaattttgc agttaatatt gatgtgttga gtcgctttag aagtaaaaaa
      901 gagcagactg caacacttga aaaattgaaa aacggtcaag tcgatatttt gattggaaca
      961 catcgtgttt tgtcaaaaga tgttgtgttt gctgatttgg gcttgatgat tattgatgag
     1021 gaacagcgat ttggtgtcaa gcataaggaa actttgaaag aactgaagaa acaagtggat
     1081 gtcctaacct tgaccgctac gccaatccct cgtaccctcc atatgtctat gctgggaatc
     1141 agagatttat ctgttattga aactccgccg actaatcgct atcctgttca gacctatgtt
     1201 ttggaaaaga atgatagtgt cattcatgat gctgtcttgc gtgaaatgga gcgtggaggt
     1261 caagtttatt atctttacaa caaagttgac acaattgttc agaaggtttc agaattacag
     1321 gagttgattc cggaggcttc gattggatat gttcatggtc gaatgagtga agtccagttg
     1381 gaaaatactc tattagactt tattgaggga caatacgata tcttggtgac gactactatt
     1441 attgagacag gggtggacat tccaaatgct aatactttat ttattgaaaa tgcggaccat
     1501 atgggcttgt caaccttata tcagttaaga ggaagagtcg gtcgtagtaa tcgtattgct
     1561 tatgcttatc tcatgtatcg tccagaaaaa tcaatcagtg aagtctctga aaagagatta
     1621 gaagcgatta aaggatttac agaattgggc tctggcttta agattgcaat gcgagatctt
     1681 tcgattcgtg gagcaggaaa tcttttagga aaatcccagt ctggtttcat tgattctgtt
     1741 ggttttgaat tgtattcgca gttattagag gaagctattg ctaaacgaaa cggtaatgct
     1801 aacgctaaca caagaaccaa agggaatgct gagttgattt tgcaaattga tgcctatctt
     1861 cctgatactt atatttctga tcaacgacat aagattgaaa tttacaagaa aattcgtcaa
     1921 attgacaacc gtgtcaatta tgaagagtta caagaggagt tgatagaccg ttttggagaa
     1981 tacccagatg tagtagccta tcttttagag attggtttgg ttaaatcata cttggacaag
     2041 gtctttgttc aacgtgtgga aagaaaagat aataaaatta caattcaatt tgaaaaagtc
     2101 actcaacggc tgtttttagc tcaagattat tttaaagctt tatccgtaac gaacttaaaa
     2161 gcaggcatcg ctgagaataa gggattaatg gagcttgtat ttgatgtcca aaataagaaa
     2221 gattatgaaa ttttagaagg tctgctgatt tttggagaaa gtttattaga gataaaagag
     2281 tttaaggaag aaaattccat ttgatatttt tcttctataa aatagataaa aatggtacaa
     2341 taataaattg aggtaataag gatgagatta gataaatatt taaaagtatc gcgaattatc
     2401 aagcgtcgta cagtcgcaaa ggaagtagca gataaaggta gaatcaaggt taatggaatc
     2461 ttggccaaaa gttcaacgga cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat
     2521 aagttgctgc ttgtaaaagt actagagatg aaagatagta caaaaaaaga agatgcagca
     2581 ggaatgtatg aaattatcag tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca
     2641 attgaataat tcttttattc aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg
     2701 acaaaaacgg aatcgtttta tgggaggggt attgattttg attatgctat tatttatctt
     2761 gccaactttt aatttagcgc agagttatca gcaattactc caaagacgtc agcaattagc
     2821 agacttgcaa actcagtatc aaactttgag tgatgaaaag gataaggaga cagcatttgc
     2881 taccaagttg aaagatgaag attatgctgc taaatataca cgagcgaagt actattattc
     2941 taagtcgagg gaaaaagttt atacgattcc tgacttgctt caaaggtgat aaaatggaaa
     3001 atttattaga cgtaatagag caatttttga gtttgtcaga tgaaaagctg gaagaattgg
     3061 ctgataaaaa tcaattattg cgtttacaag aagaaaagga aaggaagaat gcgtaaattc
     3121 ttaattattt tgttgctacc aagttttttg accatttcaa aagtcgttag cacagaaaaa
     3181 gaagtcgtct atacttcgaa agaaatttat tacctttcac aatctgactt tagtatttat
     3241 tttagagaaa aattaagttc tcccatggtt tatggagagg ttcctgttta tgcgaatgaa
     3301 gatttagtag tggaatctgg gaaattgact cccaaaacaa gttttcaaat aaccgagtgg
     3361 cgcttaaata aacaaggaat tccagtattt aagctatcaa atcatcaatt tatagctgcg
     3421 gacaaacgat ttttatatga tcaatcagag gtaactccaa caataaaaaa agcatggtta
     3481 gaatctgact ttaaactgta caatagtcct tatgatttaa aagaagtgaa atcatcctta
     3541 tcagcttatt cgcaagtatc aatcgacaag accatgtttg tagaaggaag agaatttcta
     3601 catattgatc aggctggatg ggtagctaaa gaatcaactt ctgaagaaga taatcggatg
     3661 agtaaagttc aagaaatgtt atctgaaaaa tatcagaaag attctttctc tatttatgtt
     3721 aagcaactga ctactggaaa agaagctggt atcaatcaag atgaaaagat gtatgcagcc
     3781 agcgttttga aactctccta tctctattat acgcaagaaa aaataaatga gggtctttat
     3841 cagttagata cgactgtaaa atacgtatct gcagtcaatg attttccagg ttcttataaa
     3901 ccagagggaa gtggtagtct tcctaaaaaa gaagataata aagaatattc tttaaaggat
     3961 ttaattacga aagtatcaaa agaatctgat aatgtagctc ataatctatt gggatattac
     4021 atttcaaacc aatctgatgc cacattcaaa tccaagatgt ctgccattat gggagatgat
     4081 tgggatccaa aagaaaaatt gatttcttct aagatggccg ggaagtttat ggaagctatt
     4141 tataatcaaa atggatttgt gctagagtct ttgactaaaa cagattttga tagtcagcga
     4201 attgccaaag gtgtttctgt taaagtagct cataaaattg gagatgcgga tgaatttaag
     4261 catgatacgg gtgttgtcta tgcagattct ccatttattc tttctatttt cactaagaat
     4321 tctgattatg atacgatttc taagatagcc aaggatgttt atgaggttct aaaatgaggg
     4381 aaccagattt tttaaatcat tttctcaaga agggatattt caaaaagcat gctaaggcgg
     4441 ttctagctct ttctggtgga ttagattcca tgtttctatt taaggtattg tctacttatc
     4501 aaaaagagtt agagattgaa ttgattctag ctcatgtgaa tcataagcag agaattgaat
     4561 cagattggga agaaaaggaa ttaaggaagt tggctgctga agcagagctt cctatttata
     4621 tcagcaattt ttcaggagaa ttttcagaag cgcgtgcacg aaattttcgt tatgattttt
     4681 ttcaagaggt catgaaaaag acaggtgcga cagctttagt cactgcccac catgctgatg
     4741 atcaggtgga aacgattttg atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag
     4801 gaattaagga gaagcaagta gtcggagaga tagaaatcat tcgtcccttc ttgcattttc
     4861 agaaaaaaga ctttccatca atttttcact ttgaagatac atcaaatcag gagaatcatt
     4921 attttcgaaa tcgtattcga aattcttact taccagaatt ggaaaaagaa aatcctcgat
     4981 ttagggatgc aatcttaggc atcggcaatg aaattttaga ttatgatttg gcaatagctg
     5041 aattatctaa caatattaat gtggaaaatt tacagcagtt attttcttac tctgagtcta
     5101 cacaaagagt tttacttcaa acttatctga atcgttttcc agatttgaat cttacaaaag
     5161 ctcagtttgc tgaagttcag cagattttaa aatctaaaag ccagtatcgt catccgatta
     5221 aaaatggcta tgaattgata aaagagtacc aacagtttca gatttgtaaa atcagtccgc
     5281 aggctgatga agaggaagat gaatttgtgt tacactatca aaatcaggta gcttatcaag
     5341 gatatttatt ttcctttgga cttccattag aaggtgaatt aattcaacaa atacctgttt
     5401 cacgtgaaac atccatacac attcgtcatc gaaaaacagg agatgttttg attcaaaatg
     5461 ggcatagaaa aaaactcaga cgtttattta ttgatttgaa aatccctatg gaaaagagaa
     5521 actctgctct tattattgag caatttggtg aaattgtctc aattttggga attgcgacca
     5581 ataatttgag taaaaaaacg aaaaatgata taatgaacac tgtactttat atagaaaaaa
     5641 tagataggta aaaaatgtta gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa
     5701 ttacagaagc agctaaaaaa ctaggtgctc aattaactaa agactatgca ggaaaaaatc
     5761 caatcttagt tgggatttta aaaggatcta ttccttttat ggctgaattg gtcaaacata
     5821 ttgatacaca tattgaaatg gacttcatga tggtttctag ctaccatggt ggaacagcaa
     5881 gtagtggtgt tatcaatatt aaacaagatg tgactcaaga tatcaaagga agacatgttc
     5941 tatttgtaga agatatcatt gatacaggtc aaactttgaa gaatttgcga gatatgttta
     6001 aagaaagaga agcagcttct gttaaaattg caaccttgtt ggataaacca gaaggacgtg
     6061 ttgtagaaat tgaggcagac tatacctgct ttactatccc aaatgagttt gtagtaggtt
     6121 atggtttaga ctacaaagaa aattatcgta atcttcctta tattggagta ttgaaagagg
     6181 aagtgtattc aaattagaaa gaataatctt taatgaaaaa acaaaataat ggtttaatta
     6241 aaaatccttt tctatggtta ttatttatct ttttccttgt gacaggattc cagtatttct
     6301 attctgggaa taactcagga ggaagtcagc aaatcaacta tactgagttg gtacaagaaa
     6361 ttaccgatgg taatgtaaaa gaattaactt accaaccaaa tggtagtgtt atcgaagttt
     6421 ctggtgtcta taaaaatcct aaaacaagta aagaaggaac aggtattcag tttttcacgc
     6481 catctgttac taaggtagag aaatttacca gcactattct tcctgcagat actaccgtat
     6541 cagaattgca aaaacttgct actgaccata aagcagaagt aactgttaag catgaaagtt
     6601 caagtggtat atggattaat ctactcgtat ccattgtgcc atttggaatt ctattcttct
     6661 tcctattctc tatgatggga aatatgggag gaggcaatgg ccgtaatcca atgagttttg
     6721 gacgtagtaa ggctaaagca gcaaataaag aagatattaa agtaagattt tcagatgttg
     6781 ctggagctga agaagaaaaa caagaactag ttgaagttgt tgagttctta aaagatccaa
     6841 aacgattcac aaaacttgga gcccgtattc cagcaggtgt tcttttggag ggacctccgg
     6901 ggacaggtaa aactttgctt gctaaggcag tcgctggaga agcaggtgtt ccattcttta
     6961 gtatctcagg ttctgacttt gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct
     7021 ctctttttga ggatgccaaa aaagcagcac cagctatcat ctttatcgat gaaattgatg
     7081 ctgttggacg tcaacgtgga gtcggtctcg gcggaggtaa tgacgaacgt gaacaaacct
     7141 tgaaccaact tttgattgag atggatggtt ttgagggaaa tgaagggatt atcgtcatcg
     7201 ctgcgacaaa ccgttcagat gtacttgatc ctgccctttt gcgtccagga cgttttgata
     7261 gaaaagtatt ggttggccgt cctgatgtta aaggtcgtga agcaatcttg aaagttcacg
     7321 ctaagaacaa gcctttagca gaagatgttg atttgaaatt agtggctcaa caaactccag
     7381 gctttgttgg tgctgattta gagaatgtct tgaatgaagc agctttagtt gctgctcgtc
     7441 gcaataaatc gataattgat gcttcagata ttgatgaagc agaagataga gttattgctg
     7501 gaccttctaa gaaagataag acagtttcac aaaaagaacg agaattggtt gcttaccatg
     7561 aggcaggaca taccattgtt ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta
     7621 caattgtacc acgcggccgt gcaggcggat acatgattgc acttcctaaa gaggatcaaa
     7681 tgcttctatc taaagaagat atgaaagagc aattggctgg cttaatgggt ggacgtgtag
     7741 ctgaagaaat tatctttaat gtccaaacta caggagcttc aaacgacttt gaacaagcga
     7801 cacaaatggc acgtgcaatg gttacagagt acggtatgag tgaaaaactt ggcccagtac
     7861 aatatgaagg aaaccatgct atgcttggtg cacagagtcc tcaaaaatca atttcagaac
     7921 aaacagctta tgaaattgat gaagaggttc gttcattatt aaatgaggca cgaaataaag
     7981 ctgctgaaat tattcagtca aatcgtgaaa ctcacaagtt aattgcagaa gcattattga
     8041 aatacgaaac attggatagt acacaaatta aagctcttta cgaaacagga aagatgcctg
     8101 aagcagtaga agaggaatct catgcactat cctatgatga agtaaagtca aaaatgaatg
     8161 acgaaa
//
LOCUS       DAYYQN010000075         7993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYYQN010000075 DAYYQN010000000
VERSION     DAYYQN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.076"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            172..597
                     /gene="lacA"
                     /locus_tag="ACHBRQ_001820"
     CDS             172..597
                     /gene="lacA"
                     /locus_tag="ACHBRQ_001820"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM9593435.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            628..1143
                     /gene="lacB"
                     /locus_tag="ACHBRQ_001821"
     CDS             628..1143
                     /gene="lacB"
                     /locus_tag="ACHBRQ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM9593436.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQIDANFFTEF
                     LEKWDRGEYHD"
     gene            1154..2083
                     /locus_tag="ACHBRQ_001822"
     CDS             1154..2083
                     /locus_tag="ACHBRQ_001822"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM9593437.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2085..3065
                     /gene="lacD"
                     /locus_tag="ACHBRQ_001823"
     CDS             2085..3065
                     /gene="lacD"
                     /locus_tag="ACHBRQ_001823"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9593438.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3299..4135
                     /locus_tag="ACHBRQ_001824"
     CDS             3299..4135
                     /locus_tag="ACHBRQ_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9593439.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4171..4488
                     /locus_tag="ACHBRQ_001825"
     CDS             4171..4488
                     /locus_tag="ACHBRQ_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9593440.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4490..6184
                     /locus_tag="ACHBRQ_001826"
     CDS             4490..6184
                     /locus_tag="ACHBRQ_001826"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9593441.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            6399..7805
                     /gene="lacG"
                     /locus_tag="ACHBRQ_001827"
     CDS             6399..7805
                     /gene="lacG"
                     /locus_tag="ACHBRQ_001827"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9593442.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDAPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            7848..7993
                     /locus_tag="ACHBRQ_001828"
                     /pseudo
     CDS             7848..7993
                     /locus_tag="ACHBRQ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
BASE COUNT         2567 a         1354 c         1656 g         2416 t
ORIGIN      
        1 atagaagttt tataaaaaag taaaaaataa ataacaaaaa caaacacaaa ctgttgactt
       61 cttcaaacaa tagtagtata ttatgtttgt gaggactaat tgactgtttt ttaaagacag
      121 ccaatacact gctggaatcc agcatagaaa aaataaaaag gagtatacaa tatgtctatt
      181 gttatcggtg cagatgctgc aggtttgaga ttgaaagaag ttgtgaaaga cttcttagaa
      241 aaagaaaact tccaccttgt ggatgttaca gctgaaggtc aagactttgt tgatgtgact
      301 ctcgctgttg ctgcagaagt aaacaaagaa gaacaaaacc ttggtatcgt gattgatgct
      361 tatggagctg gtccatttat ggttgcaact aagatcaaag gaatggttgc tgcagaagtt
      421 tctgatgaac gttcagctta tatgactcgt ggccacaaca actcacgtat gatcactatg
      481 ggtgcacaac ttgttggtga tgaattggct aaaaacatcg ctaaaggatt tgttaatggt
      541 aaatacgacg gtggtcgtca ccaaatccgg gttgacatgt tgaacaaaat gggctaatta
      601 gattatagta agaaaggtaa gttaaaaatg agaattgcaa ttggatgtga ccacatcgta
      661 acagatgaaa aaatggcggt ttcagaattt ttgaaatcaa aaggatatga agtcattgac
      721 tttggtacat atgaccatac acgtactcac tacccaatct ttggtaaaaa agtaggggaa
      781 gctgtaacta gcggtcaagc tgatcttgga gtatgtatct gtggtactgg tgttggtatc
      841 aacaacgctg taaataaagt tccaggtgtt cgttctgcct tggttcgtga tatgacaaca
      901 gccctttatg ctaaagaaca attgaacgct aacgttattg gttttggtgg taagattact
      961 ggtgaattgc ttatgtgtga tatcatcgaa gctttcatcc atgctgaata caaaccaact
     1021 gaagaaaaca aaaaattgat tgcgaaaatt gaacatgttg aaagtcacaa tgctcaacaa
     1081 atagacgcaa acttcttcac tgaattcctt gaaaaatggg atcgcggaga ataccacgac
     1141 taagaggtaa cctatgattt taacagttac aatgaaccca tccattgata tttcctatcc
     1201 cttggatgag ttgaagattg atactgtcaa tcgtgtggtg gatgtaacca aaacggctgg
     1261 tggtaaggga ctcaatgtta cccgagtact ttcagaattt ggcgattctg ttcttgctac
     1321 tggtttagtg ggtggcaaac ttggtgagtt tttggttgaa catatcgata atcaagtaaa
     1381 gaaagatttc ttctcaattc agggagaaac tcgtaactgt atcgctattc tccacggaga
     1441 caaccaaaca gaagttcttg aaaaaggtcc tgaagtattg gaacaggaag gtcaagactt
     1501 tttggaacat ttcaaaaaac tcttggagtc agttgaagta gtggccatct caggtagtct
     1561 gccagctggc cttccagttg attactatgc gagcttggta gaacttgcta atcaagctgg
     1621 caagcctgta gtcttggact gctcaggtgc agcacttcag gctgttcttg aatcacccca
     1681 taaaccaaca gtcatcaaac caaataatga agaattgtct cagcttcttg gaagagaagt
     1741 ttctgaggat ttggatgaat taaaagaagt acttcaagaa cctttgtttg cagggattga
     1801 atggattatc gtttcacttg gtgccaacgg tacttttgcc aaacatggtg acactttcta
     1861 caaggtagat attcctagaa ttcaggtggt aaatcctgtt ggatctggag actctactgt
     1921 ggcaggaatt tcttcaggac ttcttcacaa agaatcggat gcagaattac tcatcaaggc
     1981 aaatgtcctt ggtatgctca atgctcaaga aaaaatgact ggtcatgtca acatggccaa
     2041 ctatcaagtt ctatatgatc aattaatagt aaaagaggta taaaatggct ttaacagaac
     2101 aaaaacgtgt acgcttagaa aaactttctg atgaaaatgg tatcatctca gctcttgcat
     2161 ttgaccaacg tggtgctttg aaacgcctca tggctcaaca ccaaacagaa gaaccaactg
     2221 tggcccaaat ggaagaactt aaagtcttgg tagcagatga attgactaaa tacgcttcat
     2281 ctatgcttct tgaccctgag tatggacttc cagcaactaa agctcttgat gaaaaagctg
     2341 gtcttctcct tgcttatgaa aaaacaggtt atgacacaac aagcacaaaa cgcttgccag
     2401 actgcttgga tgtctggtct gcaaaacgta ttaaagaaga aggtgcagat gcagttaaat
     2461 tcttgcttta ctatgatgta gatagctcag acgaactcaa tcaagaaaaa caagcctaca
     2521 tcgaacgcat cggttctgag tgtgtggctg aagatatccc attcttcctt gaaattcttg
     2581 cttacgatga aaaaattgcg gatgcaggtt ctgtagaata cgcgaaagta aaaccacaca
     2641 aagttatcgg cgctatgaaa gtcttttcag acccacgctt taacattgat gtcttgaaag
     2701 ttgaagttcc tgttaacatt aaatatgttg aaggcttcgc tgaaggtgaa gtagtttata
     2761 cacgtgaaga agcagcagcc ttcttcaaag cgcaagatga agcaacgaac ttgccataca
     2821 tctacttgag tgctggtgta tcagctaaac tcttccaaga tactcttgta tttgctcatg
     2881 aatcaggtgc aaactttaac ggagttcttt gtggccgtgc aacatgggct ggatcagttg
     2941 aagcatacat caaagatggt gaaacagcag ctcgcgaatg gcttcgcaca actggatttg
     3001 aaaacattga tgagctcaac aaagttcttc aaacaacagc aacttcatgg aaagaacgtg
     3061 tgtaagaaag tcctcctagt ttaggaacat gaatctaaaa aaatttaaaa aaagttgtat
     3121 gtaaaggttt acaaaataac ttacttgtgc tatacttaaa tcacaagtta atacaaggtg
     3181 agtgttacta agtaatatta ggcatgatca caggtgaatt agaaatcagc tgattttcta
     3241 gttcatttgt ggtcattttt tgtactcata tacctttaag atataaaagg aggttgacat
     3301 gtatcgaatt ctaaatccaa tgaatcacaa tgtctcgctt gtcagaaatg ataagggaga
     3361 agaggtgatt gtaattggta aggggattgc attcggaaag aagaaggggg atttgattgc
     3421 tgaaaatcag gttgagaaaa tctttcggat gaagaccgaa gagtccagag aaaactttat
     3481 ggctcttctc aaagatgttc cgcttgattt tatcacagtg acctatgaaa tcattgataa
     3541 gctatcaaag aaatatcatt atccgattca agagtatctc tatgtaacct tgacagatca
     3601 tatttactgt tcttatcaag ctctaactca aggaaggtac aaggatagta atctgccaga
     3661 tatttctgct aagtatcctg tcgcttttca aatcgcaaat gaagcttttg aaatttactg
     3721 tcagaagcta gcagatcatt ttcctgagga cgaaattatt cggattgctt atcatttcat
     3781 taatgctgaa ggtgaaaatg aagtggaact tgtggagtcg attgataaga ggaaagaaat
     3841 tctcaggaat gttgaagaag ttttaacgga ctatgcaatt caacgaacta aaaagaataa
     3901 ccatttctat gatcgcttta tgatccattt gaattatttc ttggattatt tagacagatc
     3961 tagagatgat aaccaatcac ttctggatat ggaagatcat attaaacaat cctatccaaa
     4021 agccttcgag attggttcca agatctatga tgtgattacg caacatacgg gtcttgattt
     4081 gtataaaagt gaacgagttt atctagttct acatatccaa cgtttattgt cataaaaatt
     4141 tatttaaaac tatataagga gaattctatc atgaatagag aagaagtaac attgttaggt
     4201 tttgaaatcg tagcctatgc tggcgatgct cgttcaaaac tattggaagc cttgaaggct
     4261 gctgaagctg gtgattttga aaaagcggac gctctggtag aggaagctgg tagctgtatt
     4321 gcagaggctc accacgcgca aacaagtcta ttgactaagg aagcttctgg tgaggactta
     4381 gcgtatagtg tgaccatgat gcatggtcaa gatcacttga tgacaactat cttgttaaaa
     4441 gacctaatgc accatttaat tgaactttac aagagaggag ttaaataaaa tggataagtt
     4501 aataattttt attgagaaag gaaagccttt ctttgagaaa ttatcgagaa atatatactt
     4561 aagagcgata aaagatggtt ttatttccag tatgccagca gtattattct caagtatttt
     4621 tatactaata gctgctgtgc ccaatatttt tgggtttaaa tggtctgatg aacagttagc
     4681 ttttattttg aaaccttaca actactcaat ggggatttta gctctcttag tagctgggac
     4741 aactgcaaaa tcgttaacag attcagttaa tacacgtagt atggagaaaa ccaaccaaat
     4801 caactacatg tcaacttttt tagcagctgt tgttggattg ttaatactgg cagctgatcc
     4861 aattgaaggt ggatttgcta atggtctttt aggtactaga ggattactta ctgcattttt
     4921 ggcagctttt attacagtaa atatctataa agtatgtatt aaaaataatg taaccattag
     4981 acttcctgaa gaggttccac caaatattgc acaagttttt aaagatgtta ttccgtttgc
     5041 cttatctgtt ctttcaatat atggattgga tttaattgtt agaaatattt ttggtacaaa
     5101 tgtagcagaa tctgttggta aaatattagc ccctctattt tcagctactg atggttatat
     5161 cggtttagct attgtatttg gtgcttatgc tttcttttgg tttgttggaa ttcatgggcc
     5221 atctgttgta gaacctttaa ttgtagctat cagttatgca aatatagaag ctaatgttca
     5281 gcttgtgcaa gctggtatgc acgcagataa aattttaaat ccagttactc aaacttttgt
     5341 tgtaacaatg ggtggtactg gtgcaacact agtagttcca tttatgttta tgtggttatg
     5401 taaatcaaaa agaaatagaa ttgtcggacg tgcgtctgta gttcctactt tctttggcgt
     5461 aaatgaacca atcctatttg gtgctccaat tgttttaaat cctatcttct ttattccgtt
     5521 tgttactgct cctattatca atgtttggat tatgaaattt tttgtagatg ttctacaaat
     5581 gaatagtttc agtattattt taccttggac aacacctgct ccaattggta ttgttatggg
     5641 aacggcgctg gctccattgt cattcgtctt ggctataact ttaatcatta tcgacacttt
     5701 aatctattat ccatttgtta aagtttatga ccatcaaatt ttagaagaag aacggaaagg
     5761 aaactcttca tctgaattga aagaaaaagt tgctgcaaac ttcaacactg caaaagcgga
     5821 tgctattctt gaaaaagcag gtgtcgatgc agcacaaaat accatcactg aagaaacaaa
     5881 tgtcctcgtt ctctgtgctg gtggaggtac aagtggactc cttgcaaatg ctttgaataa
     5941 ggcagcagca gaatacaatg tccctgtgaa agcagcagca ggcggctatg gtgctcaccg
     6001 tgaaatgtta ccagagtttg atttggttat ccttgctcct caagttgctt caaactttga
     6061 agatatgaag gcagaaacag ataagctcgg tattaaacta gcgaaaacag aaggcgctca
     6121 atacatcaaa ttaactcgtg atggaaaagg tgccctagca ttcgtacaag aacaattcga
     6181 ttaaggctag gaactgtgaa acagtctcct atcgttacgg aaatcgctat ggcgaatttc
     6241 ctaatcgcct tattaattcg tcggtaaaaa gatatcgttt ttacctcctc atgtcacaat
     6301 tcggtgactt ggtacaagaa gtgagatgga gaaggatggc tcactgactc ctctcctctc
     6361 acttttactt tatttaaatc aagaaatagg tgaaaaaaat gacaaaaaca cttccaaaag
     6421 actttatttt tggtggcgca acagctgcct atcaagcaga aggtgctaca catactgatg
     6481 gtaaaggacc agtagcgtgg gataaatatc ttgaagataa ctactggtac actgcagaac
     6541 cagccagtga tttttacaat cgatatccag ttgatctcaa gctagcagaa gagtatggtg
     6601 tcaatggcat tcgaatttct attgcctggt cacgtatttt cccgactggt tatggtcaag
     6661 taaatgctaa aggtgttgag ttttatcata atttatttgc agagtgtcac aaacgtcatg
     6721 ttgagccctt tgtaactctt catcactttg acgcgccaga agctctccac tcaaatggag
     6781 acttcttaaa ccgtgaaaat atcgaacatt ttgtagacta cgctgccttc tgttttgaag
     6841 aatttccaga agtaaactat tggacaacct tcaatgaaat tggaccaatc ggtgatggtc
     6901 aatatttggt tgggaaattc cctccaggta tccagtacga ccttgccaaa gtctttcaat
     6961 cacaccacaa tatgatggtg tctcatgcat gcgcggtaaa attgtacaaa gagaaaggct
     7021 ataaagggga aattggtgtt gttcacgccc tgcctactaa atatcctcta gatcctgaaa
     7081 atccagcaga tgttcgtgca gcagagttgg aagatatcat ccacaataaa ttcatcttag
     7141 acgcaactta tctaggtcgc tattcagctg aaaccatgga aggtgtcaac catatcttat
     7201 cagtcaatgg tggtagtttg gatcttcgtg aagaagattt tacagcatta gaagctgcaa
     7261 aagacttgaa tgatttccta ggaatcaact actatatgag tgactggatg gaagcctttg
     7321 atggagaaac tgaaattatc cataatggta aaggtgaaaa aggaagctct aagtatcaaa
     7381 tcaaaggtat tggtcgtcgt gtagctcctg actatgtatc acgcacggat tgggattgga
     7441 ttatctaccc tcaaggtttg tatgaccaaa tcatgcgtgt gaagaaagat tatcctaact
     7501 acaagaagat ttacatcact gaaaatggtc tcggctataa agatgagttc gttgataaca
     7561 ctgtttacga tgatggtcgt attgattacg tgaaacaaca cttggagatc ttgtctgatg
     7621 cgattgcaga tggagctaat gtaaaaggtt acttcatttg gtcattaatg gatgtcttct
     7681 catggtcaaa cggttatgag aaacgttatg gtctcttcta cgtagatttt gaaactcaag
     7741 aacgttatcc taagaaatca gctcactggt acaagaaagt agcggaaact cagattatag
     7801 actagtagaa ttagtcatta gatatagaat tttagtgagt caaaaagatg ttcaaagatt
     7861 ttatccaatc tatttatgaa aaaaagttta tattataaat ttcgaaaaat gctctcaaat
     7921 accgtgtttg acgagtgaag aattgaaaag tcttggaaaa tggtatgtct cgactggtaa
     7981 agaatggatt tgt
//
LOCUS       DAYYQN010000076         7657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYYQN010000076 DAYYQN010000000
VERSION     DAYYQN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.077"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            176..439
                     /locus_tag="ACHBRQ_001829"
     CDS             176..439
                     /locus_tag="ACHBRQ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9593443.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            445..2178
                     /gene="ptsP"
                     /locus_tag="ACHBRQ_001830"
     CDS             445..2178
                     /gene="ptsP"
                     /locus_tag="ACHBRQ_001830"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9593444.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2433..4883
                     /gene="phtA"
                     /locus_tag="ACHBRQ_001831"
     CDS             2433..4883
                     /gene="phtA"
                     /locus_tag="ACHBRQ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HFM9593445.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            5041..7500
                     /gene="phtB"
                     /locus_tag="ACHBRQ_001832"
     CDS             5041..7500
                     /gene="phtB"
                     /locus_tag="ACHBRQ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HFM9593446.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPSPQPAPSNPIDEKLVKEAVRKVGDGYV
                     FEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDL
                     LARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKP
                     NAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLS
                     EAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQY
                     TVEVKNGSLIIPHYDHYHNIKCEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHS
                     DNGFGNASDHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPKPT
                     EEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGT
                     QDNNTIMAEAEKLLALLKESK"
BASE COUNT         2612 a         1465 c         1616 g         1964 t
ORIGIN      
        1 ctatatcctg tgttttgtta tctaggatag aagattttca gcaagttatc tgaaaggaaa
       61 ttgaaggaaa atccagaaaa tgcttgaaaa aaatcctaga agatggtata atactaaatt
      121 gtaagggtta tcacatataa ctcaaaaaaa gaaagaacaa aaggagagtc aaactatggc
      181 ttctaaagat ttccacgtag tggcagaaac aggtattcac gcacgtccag caacattgtt
      241 ggtacaaact gctagcaaat ttgcttcaga tatcactctt gagtacaaag gtaaatcagt
      301 taaccttaaa tcaattatgg gtgttatgag tcttggtgtt ggccaaggtg ctgacgtaac
      361 tatctcagct gaaggtgcag atgcagatga cgctatcgct gcaatctcag aaacaatgga
      421 aaaagaagga ttggcataag ggaaatgaca gaaatgctta aaggaatcgc agcatctgac
      481 ggtgttgcag ttgcaaaagc atatctactc gttcagccgg atttgtcatt tgagactatt
      541 acagtcgaag atacaaacgc agaagaagct cgccttgatg ccgctctaca ggcatcacaa
      601 gacgagcttt ctgttattcg cgagaaagca gtaggtacgc tcggtgaaga agcagctcaa
      661 gtttttgatg ctcacttaat ggttcttgct gacccagaaa tgatcagcca aatcaaggaa
      721 actatccgtg cgaagaaagt gaatgcagaa gcaggtctga aagaagttac agatatgttt
      781 atcactatct ttgaaggcat ggaagacaac ccatacatgc aagaacgcgc agcggatatc
      841 cgcgacgtga caaaacgtgt attggcaaac cttcttggta aaaaattgcc aaacccagct
      901 tctatcaatg aagaagtgat tgtgattgcg catgacttga caccatctga tacagctcaa
      961 ttggacaaaa actttgtaaa agcttttgta accaacattg gtggacgtac aagccactca
     1021 gctatcatgg cacgtacact tgaaattgct gctgtattag gtacaaataa catcactgaa
     1081 atcgttaaag acggtgacat ccttgctgtt aacgggatca ctggagaagt gattatcaac
     1141 ccaacagatg aacaagcggc agaatttaaa gcagctggtg aagcctatgc gaaacaaaaa
     1201 gctgaatggg cacttttgaa agatgctcaa acagtgactg ctgacggtaa acacttcgag
     1261 ttggctgcca atattggtac tccaaaagac gttgaaggtg ttaacaacaa cggtgcagaa
     1321 gctgttggac tttaccgtac agagttcttg tacatggatt ctcaagactt cccaactgaa
     1381 gatgagcagt atgaagcata caaggctgtt cttgaaggaa tgaacggtaa acctgttgtc
     1441 gttcgtacaa tggatatcgg tggagataag gaacttcctt acttcgatat gcctcacgaa
     1501 atgaacccat tccttggatt ccgtgctctt cgtatctcta tctctgagac tggagatgct
     1561 atgttccgca cacaaatccg tgctcttctt cgtgcgtctg ttcacggtca attgcgtatc
     1621 atgttcccaa tggttgcgct cttgaaagaa ttccgtgcag cgaaagcagt ctttgatgaa
     1681 gaaaaagcaa accttcttgc tgaaggtgtt gcagttgcgg ataacatcca agttggtatc
     1741 atgatcgaga ttcctgcagc ggctatgctt gcagaccaat ttgctaaaga agttgacttc
     1801 ttctcaattg gtacaaacga cttgatccaa tatacaatgg cagcagaccg tatgaacgaa
     1861 caagtttcat acctttacca accatacaac ccatcaatcc tacgcttgat taacaatgtg
     1921 atcaaagcag ctcacgctga aggtaaatgg gctggtatgt gtggtgagat ggctggtgac
     1981 caacaagctg ttccacttct tgtcggaatg ggcttggatg agttctctat gtcagcaaca
     2041 tctgtacttc gtacacgcag cttgatgaag aaactcgaca cagctaagat ggaagagtac
     2101 gcaaaccgtg cccttacaga atgctcaaca atggaagaag ttcttgaact tcaaaaagaa
     2161 tacgttaatt ttgattaatc gaaaagtccc tgcaactcag ttacagggac tttttttgat
     2221 attttaaaaa gaattttcaa gaaaatcttt cttataaaaa gtccaacctt gaaaaagtag
     2281 tggtcagaac aaaaaatact taaatggttc ataaaattct tgacaagttg gagatttagg
     2341 agtaaactat taaccagtta agtaatagag aggagtttct gcaatttaga aatgaattgc
     2401 aactagaaat atcaaataga aagagagttt cgatgaaaat taataagaaa taccttgttg
     2461 gttctgcggc agctttgatt ttaagtgttt gttcttacga gttgggactg tatcaagcta
     2521 gaacggttaa ggaaaataat cgtgtttcct atatagatgg aaaacaagcg acgcaaaaaa
     2581 cggagaattt gactcctgat gaggttagca agcgtgaagg aatcaatgct gagcaaatcg
     2641 tcatcaagat aacagaccaa ggctatgtca cttcacatgg cgaccactat cattattaca
     2701 atggtaaggt tccttatgat gcgattttca gtgaagaatt actcatgaaa gatccaaact
     2761 ataagctaaa agatgaggat attgttaatg aggtcaaggg tggatatgtt atcaaggtag
     2821 atggaaaata ctatgtttac cttaaggatg ctgcccacgc ggataacgtc cgtacaaaag
     2881 aggaaatcaa tcgacaaaaa caagaacata gtcaacatcg tgaaggtggg actccaagaa
     2941 acgatggtgc tgttgccttg gcacgttcgc aaggacgcta tactacagat gatggttata
     3001 tctttaatgc ttctgatatc atagaggata ctggtgatgc ttatatcgtt cctcatggag
     3061 atcattacca ttacattcct aagaatgagt tatcagctag cgagttggct gctgcagaag
     3121 ccttcctatc tggtcgggga aatctgtcaa attcaagaac ctatcgccga caaaatagcg
     3181 ataacacttc aagaacaaac tgggtacctt ctgtaagcaa tccaggaact acaaatacta
     3241 acacaagcaa caacagcaac actaacagtc aagcaagtca aagtaatgac attgatagtc
     3301 tcttgaaaca gctctacaaa ctgcctttga gtcaacgaca tgtagaatct gatggccttg
     3361 ttttcgaccc agcgcaaatc acaagtcgaa cagctagagg tgttgcagtg ccacacggag
     3421 atcattacca cttcatccct tactctcaaa tgtctgaatt ggaagaacga atcgctcgta
     3481 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaaggcca gaacaaccaa
     3541 gtccacaacc gactccggaa cctagtccag gcccgcaacc tgcaccaaat cttaaaatag
     3601 actcaaattc ttctttggtt agtcagctgg tacgaaaagt tggggaagga tatgtattcg
     3661 aagaaaaggg catctctcgt tatgtctttg cgaaagattt accatctgaa actgttaaaa
     3721 atcttgaaag taagttatca aaacaagaga gtgtttcaca cactttaact gctaaaaaag
     3781 aaaatgttgc tcctcgtgac caagaatttt atgataaagc atataatctg ttaactgagg
     3841 ctcataaagc cttgtttgaa aataagggtc gtaattctga tttccaagcc ttagacaaat
     3901 tattagaacg cttgaatgat gaatcgacta ataaagaaaa attggtagat gatttattgg
     3961 cattcctagc accaattacc catccagagc gacttggcaa gccaaattct caaattgagt
     4021 atactgaaga cgaagttcgt attgctcaat tagctgataa gtatacaacg tcagatggtt
     4081 acatttttga tgaacatgat ataatcagtg atgaaggaga tgcatatgta acgcctcata
     4141 tgggccatag tcactggatt ggaaaagata gcctttctga taaggaaaaa gttgcagctc
     4201 aagcctatac taaagaaaaa ggtatcctac ctccatctcc agacgcagat gttaaagcaa
     4261 atccaactgg agatagtgca gcagctattt acaatcgtgt gaaaggggaa aaacgaattc
     4321 cactcgttcg acttccatat atggttgagc atacagttga ggttaaaaac ggtaatttga
     4381 ttattcctca taaggatcat taccataata ttaaatttgc ttggtttgat gatcacacat
     4441 acaaagctcc aaatggctat accttggaag atttgtttgc gacgattaag tactacgtag
     4501 aacaccctga cgaacgtcca cattctaatg atggatgggg caatgccagt gagcatgtgt
     4561 taggcaagaa agaccacagt gaagatccaa ataagaactt caaagcggat gaagagccag
     4621 tagaggaaac acctgctgag ccagaagtcc ctcaagtaga gactgaaaaa gtagaagccc
     4681 aactcaaaga agcagaagtt ttgcttgcaa aagtaacgga ttctagtctg aaagccaatg
     4741 caacagaaac tctagctggt ttacgaaata atttgactct tcaaattatg gataacaata
     4801 gtatcatggc agaagcagaa aaattacttg cgttgttaaa aggaagtaat ccttcatctg
     4861 taagtaagga aaaaataaac taatgaaaaa tgaaagtctc gataaagagg ctttcatttt
     4921 tattatgtat atatgtaaaa ttcttgacaa gcaatattaa aaagagtaaa ctattaacta
     4981 gttaattaac cggtttatta ctttatagtg aatcaaatat acttaagaaa agaggaaaga
     5041 atgaaaatca ataaaaaata tctagcaggt tcagtggcag tccttgccct aagtgtttgt
     5101 tcctatgagc ttggacgtta ccaagctggt caggataaga aagagtctaa tcgagttgct
     5161 tatatagatg gtgatcaggc tggtcaaaag gcagaaaact tgacaccaga tgaagtcagt
     5221 aagagggagg ggatcaacgc cgaacaaatt gttatcaaga ttacggatca aggttatgtg
     5281 acctctcatg gagaccatta tcattactat aatggcaagg ttccttatga tgccatcatc
     5341 agtgaagagc tcctcatgaa agatccgaat tatcagttga aggattcaga cattgtcaat
     5401 gaaatcaagg gtggttatgt cattaaggta aacggtaaat actatgttta ccttaaggat
     5461 gcagctcatg cggataatat tcggacaaaa gaagagatta aacgtcagaa gcaggaacgc
     5521 agtcataatc ataactcaag agcagataat gctgttgctg cagccagagc ccaaggacgt
     5581 tatacaacgg atgatgggta tatcttcaat gcatctgata tcattgagga cacgggtgat
     5641 gcttatatcg ttcctcacgg cgaccattac cattacattc ctaagaatga gttatcagct
     5701 agcgagttag ctgctgcaga agcctattgg aatgggaagc agggatctcg tccttcttca
     5761 agttctagtt ataatgcaaa tccagctcaa ccaagattgt cagagaacca caatctgact
     5821 gtcactccaa cttatcatca aaatcaaggg gaaaacattt caagcctttt acgtgaattg
     5881 tatgctaaac ccttatcaga acgccatgtg gaatctgatg gccttatttt cgacccagcg
     5941 caaatcacaa gtcgaaccgc cagaggtgta gctgtccctc atggtaacca ttaccacttt
     6001 atcccttatg aacaaatgtc tgaattggaa aaacgaattg ctcgtattat tccccttcgt
     6061 tatcgttcaa accattgggt accagattca agaccagaac aaccaagtcc acaaccgact
     6121 ccagaaccta gtccaagtcc gcaaccagct ccaagcaatc caattgatga gaaattggtc
     6181 aaagaagctg ttcgaaaagt aggcgatggt tatgtctttg aggagaatgg agtttctcgt
     6241 tatatcccag ccaaggatct ttcagcagaa acagcagcag gcattgatag caaactggcc
     6301 aagcaggaaa gtttatctca taagctagga gctaagaaaa ctgacctccc atctagtgat
     6361 agagaatttt acaataaggc ttatgactta ctagcaagaa ttcaccaaga tttacttgat
     6421 aataaaggtc gacaagttga ttttgaggct ttggataacc tgttggaacg actcaaggat
     6481 gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc tccgattcgt
     6541 catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga tgagattcaa
     6601 gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga tcctcgtgat
     6661 ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag ccactggatt
     6721 aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc taaagagaaa
     6781 ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc aaaaggagca
     6841 gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg catgccttac
     6901 aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca ttatgaccat
     6961 taccataaca tcaaatgtga gtggtttgac gaaggccttt atgaggcacc taaggggtat
     7021 actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa cgaacgtccg
     7081 cattcagata atggttttgg taacgctagc gaccatgttc aaagaaacaa aaatggtcaa
     7141 gctgatacca atcaaacgga aaaaccaagc gaggagaaac ctcagacaga aaaacctgag
     7201 gaagaaaccc ctcgagaaga gaaaccgcaa agcgagaaac cagagtctcc aaaaccaaca
     7261 gaggaaccag aagaatcacc agaggaatca gaagaacctc aggtcgagac tgaaaaggtt
     7321 gaagaaaaac tgagagaggc tgaagattta cttggaaaaa tccaggatcc aattatcaag
     7381 tccaatgcca aagagactct cacaggatta aaaaataatt tactatttgg cacccaggac
     7441 aacaatacta ttatggcaga agctgaaaaa ctattggctt tattaaagga gagtaagtaa
     7501 aggtagcagc attttctaac tcctaaaaac aggataggag aacgggaaaa cgaaaaatga
     7561 gagcagaatg tgagttctag ttctcatttt tttcatgaaa atgtgcaaaa tatagtagat
     7621 tgaaactaga atagtatacc tctacttcta aaacatt
//
LOCUS       DAYYQN010000077         7619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYYQN010000077 DAYYQN010000000
VERSION     DAYYQN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.078"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..282)
                     /locus_tag="ACHBRQ_001833"
     CDS             complement(<1..282)
                     /locus_tag="ACHBRQ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HFM9593447.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAK"
     gene            complement(825..1718)
                     /locus_tag="ACHBRQ_001834"
     CDS             complement(825..1718)
                     /locus_tag="ACHBRQ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9593448.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(1739..1933)
                     /locus_tag="ACHBRQ_001835"
     CDS             complement(1739..1933)
                     /locus_tag="ACHBRQ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593449.1"
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPL
                     FSLIVLYMIDKRIRKKKKSL"
     gene            complement(2344..2469)
                     /locus_tag="ACHBRQ_001836"
     CDS             complement(2344..2469)
                     /locus_tag="ACHBRQ_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HFM9593450.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            complement(2472..2702)
                     /gene="blpU"
                     /locus_tag="ACHBRQ_001837"
     CDS             complement(2472..2702)
                     /gene="blpU"
                     /locus_tag="ACHBRQ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HFM9593451.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRIWQGAATGAVGGAILGGVAYAATCWW"
     gene            complement(2905..3195)
                     /locus_tag="ACHBRQ_001838"
                     /pseudo
     CDS             complement(2905..3195)
                     /locus_tag="ACHBRQ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3241..3474)
                     /locus_tag="ACHBRQ_001839"
     CDS             complement(3241..3474)
                     /locus_tag="ACHBRQ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9593452.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(3480..4472)
                     /gene="plsX"
                     /locus_tag="ACHBRQ_001840"
     CDS             complement(3480..4472)
                     /gene="plsX"
                     /locus_tag="ACHBRQ_001840"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9593453.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(4469..5239)
                     /gene="recO"
                     /locus_tag="ACHBRQ_001841"
     CDS             complement(4469..5239)
                     /gene="recO"
                     /locus_tag="ACHBRQ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9593454.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(5236..6405)
                     /locus_tag="ACHBRQ_001842"
     CDS             complement(5236..6405)
                     /locus_tag="ACHBRQ_001842"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9593455.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6554..7564
                     /locus_tag="ACHBRQ_001843"
     CDS             6554..7564
                     /locus_tag="ACHBRQ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFM9593456.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
BASE COUNT         2202 a         1797 c         1210 g         2410 t
ORIGIN      
        1 tttagcagca cctttctttt tcaatatata aagaactgtt gctgcaatac ccgaaccaat
       61 agaacctact cctgtgattg cacctacaat tgaaattact gctgtaactg tactataagc
      121 actaatcaaa ttgactacag tagcagcagc ttttgtcgaa attcctaatg tcccagccaa
      181 cataggaaaa gaaacaacac ctaagcttaa ccaactaatg actgctaaca ttgctgaaac
      241 aataagaaat ttattatgaa attccattct tttagatttc attttagtgt actccttttg
      301 tatattatgt atgttttatt gagagttaat tcgcacatgt tgtattccta actctcttca
      361 aatgttcgtc tatattttat actactttta ttaacatatc tataataaat aaattactac
      421 tacattttcc tagattatca aattactttt atatcactaa gactcaataa aagataatgt
      481 ctatttaact tttatacgat tactacctcc tttttagttt acacttccta catcataaat
      541 aaacatactc aaccctcaca atctccaaat aactcttcta tatcattgaa aattgagttt
      601 catcctctat actaatgtag gaaacctatt aaaacattcc tttaatccaa tataagaata
      661 ccttgttcca ttaatctcta cccgatatta tagatagaac caaattccta tacttaaaag
      721 gacaaataac gttccaccca taataaacct cctttctttt tgagagcgtt ttctttatag
      781 gtttagtata gcaaaaacat gaaaaaaaca acatgactta gaagtcaggt tgttgggaaa
      841 tctatctgtt tttctgctaa aattctctga actaatacct gatcgtctag caattctgct
      901 agagaaatcg ccttttcata gaaactttct gcctctaaaa tattcttgtc tacaaataaa
      961 gcatattttg cctttagcaa aaatagatga ggtcggtaaa aaacatgtct tgttttagca
     1021 ataattttct caaatgtttg aatatatatc agactatctt ctgtctgaat ttggataaat
     1081 aggctcagaa gaactttttc catttgaact aggctagcct tatcttcata ttctgaacat
     1141 agcaatactt tttgagctaa ctcctcaaaa tgttctttat cttctaatcc catagcacaa
     1201 caaaagaaat acaattcaat aatcaacaaa tcatttacag aaaaatattt cttttttaat
     1261 aactgctgga aatattcttc cactaaccct aaaccgtagg tcacatctga actaccataa
     1321 atatcaaaac gagcttgtgt cacatccaca attaactgct cttcctcagg caaattctca
     1381 taatagtttt catatatttc ctcaagtata gcttcttttc gttcgattct tcccttatca
     1441 gcgtaggttt gagaatgaat cagctcatct ttgagtttta ggaatccttt tggaatctct
     1501 ataaccttaa catccacaat attctgtaat gatactccaa gtttatccgc aatcaataat
     1561 aatttaggaa gtgttgctaa agcttgccca ttttcaatac gtgctagctg acgaatcgtc
     1621 aactctgatt catcaccaca aatatctatc tgagtttttc cacaagatcg cctaatagtt
     1681 ttgattttat aaccaatttc acgtttcaac tcatccatag taatactcct taagttgatt
     1741 acaaagattt ctttttcttc ctaattcttt tgtcaatcat atataaaacg ataagcgaaa
     1801 acagcggtag aacaatataa ctcttttcac tcattatcag ctgaaatccc agggttgcta
     1861 gtatcttaaa gatgaaaata ttcatcaaaa atagaaaaac aaatactaaa aaatatttta
     1921 aaaccttctt catttctttt cttcaacttt cctaatttct acacctattt tatcgaaaat
     1981 atatataaaa ctccaaataa acacttcgac tgcacttttt ttggcaaaaa atagacagaa
     2041 caaacatcct acctactaaa aatatgatat gaaaggaaaa tgatttttta ctgacaccct
     2101 aggcaagctt gggacaaata aatcattgat agcaacattt acgacatcct atttaaagtt
     2161 ccttcaattt ctaatgtccc cgctatttct aagccacctc tggtatgatc tattcctagt
     2221 cagaatagat aacaccgttc taaattacga ttcatagtta ctcctttttt gactcgttac
     2281 tataagactt ctatttacat gcgcaaaata attttgattc agattcatca tttatagttt
     2341 tgattatttt ttaacagatt tttctgtttt cttctccgaa gactttaaaa atttttttct
     2401 aattaaatca tccatataag gaccaaatat accaactact aaaccaataa taaaactttt
     2461 aaaatccata attaccacca acatgtcgct gcataggcca cacctccaag tatagctcct
     2521 cccacagcac cagttgctgc accttgccat attcttgttt taattcctag ctgaagacct
     2581 cgtgctgctc ctcctccaac acctgctttg gcaaaatctc cccaattgca tccgccacct
     2641 tcaacgcaag caagcatctc agtatccata atttcaaatt gtgacatcgt ttttgtattc
     2701 ataacgaata cctctttttt atttttaata tttgtcttgt tacaaacttg acaagtttag
     2761 tataacagta tctattaatt tttttcatcc aaatcttgaa ttggcatcga aacgtcttga
     2821 attagctttt ttgtttcaaa atcatctcta tttttaaaaa agatgttttc taatcacttt
     2881 tttactatta gacttcctgc aaaactagaa tcctagttca tgattgataa taccagcaat
     2941 caaattcatt cgtaatccga agcgtttacg atgatttcga taggttgttg aaaacatttt
     3001 aaacgttttt actttggcaa agatattctc aaccttgctt ctctccttag atagcgcatg
     3061 attacaggct ttatcttcaa ctgttagcgg cttgagtttg ctggatttac gtggagtttg
     3121 tgcttgagga tatatcttca tgagcccttg ataaccactg tcagccaaga tttttccagc
     3181 ttgtccgata tttctcattt ctaaaaacca tctacttccg ttgacttgga actccgattg
     3241 ctattttcct tgaatgattt taaccacatc tcctacgttt tggagttggt caatttcttc
     3301 atcgctgatt tcgatactaa attcatcttc cagagtcaag ataaactcca tcaagtcaac
     3361 agaatccgca tccaaatcgt ctttcagact caaggattct gtcacgacaa agtcctctcc
     3421 ctgtcgctct tggataatgg tcacaatacg gtcaaaaatt tctttttctg tcatctcttt
     3481 tattctcctg aaaattcacg cgcagtctgg gcaactacgt ctgtttctag catggtacga
     3541 atctggcgaa tcgtactata aacagccttg gcatcgcttg agccatgagt cttgacaaca
     3601 ggtgccttaa caccaaacaa gaccgctcca ccaacatctg aataattgag ctgttttttc
     3661 aaaccactga ggctgtcctt gagaaggagg gcacctagtt tcgctcgaag accaccacct
     3721 gtaatagctg tcttgagcaa gcccatgatt cccatagctg tcccttcgat ggatttgagc
     3781 acagcgtttc ccgtgaaacc atctgccaca acaacatctg caacgccatt catcaaatca
     3841 cgcgcttcca cgtttccgat aaagttcaaa ctttcatcag ccgccagtaa ttcataagtt
     3901 tccttacgaa gcgggtcgcc cttgctactc tctgttccgt tgttgagcaa accaacgcgt
     3961 ggttgcgcaa tgccacggac atttttagca tagaaggaac ctagaaccgc atattgatgg
     4021 aggtgctggg ctgtattttc tgcattggca ccaaggtcta gcatgtcaaa accttttcca
     4081 tcaacggtag gcaaggtaga catgagtcca ggacggtcga tattcttgat acgacccacg
     4141 atgaagaatc cagcagccaa caaggcacct gtattcccag ccgaaaggac agcgtctgct
     4201 tcaccatctt tgacagcctt ggctgccaat accatactgg catttttctt attcctaata
     4261 gctctcgtag gttcatcatc cgaatcaatc ttctcatccg tatggataat gctgacgcgc
     4321 tctgtcgctg tcagatattg cttgatttta gcttcatctc cgtaaagttg aacctcgata
     4381 tctgaaaagt cagatagggc ttgattgaca ccctcaacaa tggcctgagg tgcgtaatcg
     4441 ccccccatgg catctactgc gatttttttc atttcttttc ctcttttagt aattgtcccc
     4501 agtctgctag ggaatcaata aatttctttg attttaggtg aatcccaacg tactcttcat
     4561 ataattgatc cataaattgg cgtagctctt gcttgattcc aggcttgagc gaaatggtct
     4621 ccaaagtctc aaaatcaata gcttgaaatt gattgagcag ataggggata ttgggattga
     4681 gatgacaacg tctcttatcc tcatgataat gctctggaca gaggcaggct ccatatttga
     4741 aagaaaagtc aaaagcctga ccaacccgat ggcagaagac acactcatta aaattgaggc
     4801 tgattccaaa tcgagtcaaa atttgaattt caaaaatatt ggtcaaaacc tgataatcca
     4861 agcctgcttc catcaactcc aaagtctttt gcaaaaaagc aaacaaggga gcatcctgct
     4921 gattgtcctg caaactagca tctgcaagag ctgccacata ggtcgcatag gccatgacaa
     4981 agaggtcact attaatcttg ggaaaagtca tgacctcatg atagtcttcg atgtaactga
     5041 gtccgtcatc attgattcgc aatagaaatc gtgccagcac caagggctga ataacaggcg
     5101 ccagcttaga ctgaccagcg tgtttgacaa aaaacatgcg tttgccaacc tgctctgtaa
     5161 aaattttgac gagcttgtca tcctcacgaa aattgcgatt gtaaagcacc aagccttgac
     5221 tcgtgataga ctgaatcatg cttctctcat gtactcctca agtcgtttca tggcttcttt
     5281 gatagtctcc atgctggctg cataagatag gcggacgtag ccttccccgt aacgtccaaa
     5341 ggctgcacca gggataaagg caacggcctt cttctgagca aaatccttca gaaaagcaaa
     5401 ggagtcttga ttgtagcccg ctggaatttt agcaaaaata tagaaggcac cgtctggttt
     5461 gataatctca aaaccaagag cagtcatttt ttcgatgata tagtcccgac gctggatata
     5521 ttccttcttc atgggctccg catcattttt accagccgtc aaggcttcta ccgcagcatg
     5581 ttgcgccatg gtatttgcgg cagtgaccaa gtattggtga ctcttgatta actgggctgt
     5641 gaaggccgca ggagcgaaaa tcagccccaa acgccaacct gtcatggcat gcgatttaga
     5701 caaaccattg ataataatag cctggtctct caacatcgtt cctagagaca catgggcttc
     5761 gcctgtgtag gtcaattctg agtaaacctc atcacagaca acaaaaattt cgtacttgcg
     5821 taaaacagct gccaaggcct ctaactgctc tcgactgtag gtaattcctg tcggattggc
     5881 tggatagttg agaataaccg ccttgagctt atcaccctgc tccaaaatgg ccttctccaa
     5941 catctcagga gtcaagacaa aaccattttc agtcgtatca atctcaacaa tttctgcccc
     6001 aactaagtta acaatcggtt catagcctgg ataagcagga gctggcaaaa gtaccttgtc
     6061 gccctcttcc aaaatagccg tcaaagtcgc agataaagcc tctgtcgccc caattgtaac
     6121 caagatttca ttttcaggag catagtccag ttggtacttt tccttaacaa agtcactggc
     6181 tgcctgacgt agagtcagca gaccactcat ccctgtatag taggattggt tctgatcaat
     6241 cgctcgcttg gccgcctcct tgacatggtc tggcgttgta aaatcaggtt cccccaaggt
     6301 caaacgcaag accccaggaa tctccgaaat agcctggtca aactgacgaa tcaacgaaac
     6361 ttgaatttta tctaactgtt tattaaagcg cttagttaag tccatagata cctcctactt
     6421 tcaaaacgta tctctattat actacaaaag ccccccgacc gcaaaaatac attagagaat
     6481 agctttccac tttctatttt acttttcctg tttacaggtc tataatggta acagattcta
     6541 tttggaggtt tttatgtcat ttctatcaaa aaatggagca ggcatcttgg tctgccttct
     6601 catttccatc ctatcttggt acttaggagg attcttccct gtgattggcg cacccgtttt
     6661 tgcgattttt atgggcatgc tcctacatcc ctttctctcg tcttataaac aactggatgc
     6721 tggattgacc tttagttcca agaaattgct ccagtatgcc gttatcttac ttggttttgg
     6781 tctcaatatc tctcaagtct tcgcagttgg gcaatcttca ctccctgtca tcctttccac
     6841 catttcaata gccttgattg ttgcctacct cttccagcgc ttctttgccc tggatacaaa
     6901 actggctacc ttgattggag taggttcttc tatctgtggg ggttctgcca ttgcagcgac
     6961 agcgcctgtt atccatgcta aggaaaagga agtagcccaa gccatctccg ttatcttttt
     7021 cttcaatgtc ttggctgcgc tcatctttcc aaccctcggc acctggcttc atctatccaa
     7081 tgacggcttc gccctctttg caggaactgc ggtcaatgat acttcctctg taaccgccac
     7141 agctagcgct tgggacagtc tctaccagac caataccctc gaatctgcaa ccattgtcaa
     7201 actcacgcgc accctagcta tcatccccat cactctcttt ctctcctact ggcaaagtcg
     7261 cgagcaaaaa aataagcaag gcttacaact gaaaaaagtc ttcccacttt ttatccttta
     7321 cttcatcctt gcatcgctcc taaccaccct tctgacctct ctcggtgtgt ctagtagttt
     7381 ctttacccct ctcaaacaac tctctaaatt cctcattatc atggccatga gtgccatcgg
     7441 tctcaaaact aatctggtcg ctatggtcaa atccagtgga aaatccattg ttctcggagc
     7501 cgtctgctgg attgccatca tcctcaccag ccttggtatg cagaccctta tcggcatttt
     7561 ctaatactct tcgaaaatct cttcaaacca cgtcaacgtc gccttgccgt atatatgtt
//
LOCUS       DAYYQN010000078         7410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYYQN010000078 DAYYQN010000000
VERSION     DAYYQN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.079"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            74..319
                     /locus_tag="ACHBRQ_001844"
     CDS             74..319
                     /locus_tag="ACHBRQ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593457.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFIKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            720..1712
                     /locus_tag="ACHBRQ_001845"
     CDS             720..1712
                     /locus_tag="ACHBRQ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9593458.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="ACHBRQ_001846"
     CDS             1714..2532
                     /locus_tag="ACHBRQ_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593459.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="ACHBRQ_001847"
     CDS             2534..3319
                     /locus_tag="ACHBRQ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9593460.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3636
                     /locus_tag="ACHBRQ_001848"
     CDS             3358..3636
                     /locus_tag="ACHBRQ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFM9593461.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="ACHBRQ_001849"
     CDS             3621..3818
                     /locus_tag="ACHBRQ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9593462.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(4136..4543)
                     /locus_tag="ACHBRQ_001850"
     CDS             complement(4136..4543)
                     /locus_tag="ACHBRQ_001850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593463.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDGTDVVSSKHCFEVVDGTDVVS
                     SKHCFEVVDGTDVVSSKHCFEVVDGTDVVSSKHCFEVVDRTDEVSSKHCFEVVDRTDE
                     VSSKHCFEVVDRTDEVSNHTHGKAALTWFEEIF"
     gene            4776..>7410
                     /locus_tag="ACHBRQ_001851"
     CDS             4776..>7410
                     /locus_tag="ACHBRQ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFM9593464.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARVKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDT"
BASE COUNT         2372 a         1230 c         1506 g         2302 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgcatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tttccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ttggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccga aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactagatt tgtaagctta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3841 gagtgccata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggagttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     4021 caattatgcc tgttttcgtg tttccggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactcttaga
     4141 aaatctcttc aaaccacgtc aacgccgcct tgccgtgcgt atggttactg acttcgtcag
     4201 ttctatccac aacctcaaaa cagtgttttg agctgacttc gtcagttcta tccacaacct
     4261 caaaacagtg ttttgagctg acttcgtcag ttctatccac aacctcaaaa cagtgttttg
     4321 agctgactac gtcagttcca tctacaacct caaaacagtg ttttgagctg actacgtcag
     4381 ttccatctac aacctcaaaa cagtgttttg agctgactac gtcagttcca tccacaacct
     4441 caaaacagtg ttttgagctg actacgtcag ttccatctac aacctcaaaa cagtgttttg
     4501 agcaacctgc ggctagtttc ctagtttgct ctttgatttt cattgagtac taatcttact
     4561 ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat taaatttttt
     4621 taaatgatat actgttttta tagagagata acaattatat ataaatgtac tactctattt
     4681 cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa agtttattat
     4741 ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca gtattgtcat
     4801 taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc ttagcagaca
     4861 catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta ttagataagg
     4921 aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact tcacaggatt
     4981 ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa aatcctgtga
     5041 cagacaataa cactagcaat gaagaagcaa gagtcaaaga agattccaat caatcccaag
     5101 gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa gaagataaag
     5161 ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc aagcaactat
     5221 ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac ggtagtgcca
     5281 tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt tcatcggttg
     5341 aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt ggagttgagg
     5401 aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt gatggtagag
     5461 gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct atgagaatcg
     5521 atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga actgataaaa
     5581 attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt ggcaaaatca
     5641 ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg catattgcag
     5701 ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc atagatggaa
     5761 ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga tctgggtttg
     5821 cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac gttgatgttg
     5881 tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat tggcaagcta
     5941 ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac tatgcgactt
     6001 ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg accgacactg
     6061 gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct gctaaaaatc
     6121 aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac agaaatatag
     6181 gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa gctcctagta
     6241 aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt ttggatctta
     6301 ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat gcttttaaaa
     6361 aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat tactacaata
     6421 gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt actaaaagtc
     6481 aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt aatcctgata
     6541 aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg gagcaatact
     6601 atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac gaaaaagaga
     6661 ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat atcatcgttc
     6721 cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc gatgtttcag
     6781 cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca acttatggct
     6841 atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt ttgattagac
     6901 cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga gatgacaaaa
     6961 tagatcttac aagtcttaca aaaattgctc tacaaaatac tgcgcgacct atgatggatg
     7021 caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag ggagcaggcc
     7081 taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa aacacggatt
     7141 ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa ggtgataaaa
     7201 aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt aaagtttcag
     7261 catcagcgat aactacagat tctttaactg acagattaaa acttgatgaa acatataaag
     7321 atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa aaagtcaaag
     7381 gagcaaatat cacatttgag catgatactt
//
LOCUS       DAYYQN010000079         7229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYYQN010000079 DAYYQN010000000
VERSION     DAYYQN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.080"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            165..341
                     /locus_tag="ACHBRQ_001852"
     CDS             165..341
                     /locus_tag="ACHBRQ_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593465.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            742..2955
                     /locus_tag="ACHBRQ_001853"
     CDS             742..2955
                     /locus_tag="ACHBRQ_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HFM9593466.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(3177..3653)
                     /locus_tag="ACHBRQ_001854"
     CDS             complement(3177..3653)
                     /locus_tag="ACHBRQ_001854"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9593467.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQR
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            3876..7079
                     /locus_tag="ACHBRQ_001855"
     CDS             3876..7079
                     /locus_tag="ACHBRQ_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HFM9593468.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
BASE COUNT         2398 a         1145 c         1555 g         2131 t
ORIGIN      
        1 ttccacaata aaacgcatat tatcagatgg ttaatgcgtt ttatatttgg gtttgaggct
       61 tataaaatag gataagcgta ctgaaaactt agatatgaca aacaaaataa aatttttaaa
      121 agctacagag aaacatggta taatgtatct aaaggaggta gcatatgtcc ttagatatag
      181 ataaagaaaa gatgacaatc atgggaatag cttttgaaaa ccgatccgtt tttaagagtg
      241 tttggtatgc tttaagtaca aatatgattg aaggatggcg acctacggtt agtgatgttg
      301 aaaaattacg agatgaagct cttgctctgg ggatgactta atgaagcgca actgttatga
      361 gtcttatgac tatgttgatc cggataattt atataccgat ccgggttctt cagttctaag
      421 aaataagcag gaaggaaaaa agcacgtgag cttgaatatc gtatgattgc tagtaagagt
      481 ttgaaattat ttatcaatcc tatcttagtt aatagtttaa aagtagtttg tttcacaact
      541 gcataagctt agaaaagttg aaaagtgata actctttaat aaactcttgg tttgttaaaa
      601 gatattttgt ccactacatt ttttcaattg actattattt gtcatagata taacagatag
      661 aacattgcat tcagaataat gaaaaacgca tcaaaaatgg tgcgttctgt ttttatgcta
      721 ccataaaagg aggaaaacaa attgaaaatt tttaagggag agttttatcg aatctctgta
      781 ttaacagaca agctagtaag gttagaatac tctcgaactg gaaattttga ggatagaacg
      841 acacaactta tctataatag agattttggc caaatttcgt tagattatat cgagacatca
      901 aacgtactag atattatgac ggactatttt catctgcact ttaataaagg agaatttaac
      961 gccgaaaatt tatttataga attaaaagga aattttgccg tatatggtag tcgctggtat
     1021 tttggtgaat ctattgaaac gttaaaagga acagctcgga ctctggataa ggcagatgga
     1081 gcaatctcgt tagaagatgg aattattagc cgaaatggta tagccttatt ggatgattct
     1141 caaggattta tttgggatga acaatctggt tatattgaga gagaaaatca aattgacctg
     1201 tatttctttg cctatgggca tgattataga ggagcaatca gagactttta ccatttgact
     1261 ggttcaacac ccttgttgcc aagatatgct ttaggcaatt ggtggagtag atattggcct
     1321 tatacgtcgg atgaatactt ggatttaata gacagatttg aaacagagaa aattccatta
     1381 tctatcggtg tgttagatat ggattggcat ataactgaca ttccagctcg ttttggaagt
     1441 ggctggacag gatatagttg gaatagaaac ttaataccaa atccagaaca gttattgcaa
     1501 caacttcatg atagaaagtt aaaactctcc ttaaatgtcc atcctgctga tgggatacgg
     1561 gcttatgaag aagcttatcc tcaaatcgca aaacggttgg ggttaaatgt agaactagaa
     1621 gaacctgcta tttttgattt ttttaatccc tcttttaggg aagcctactt taaagatgtt
     1681 cattatgagc tagaaaagca gggagtagat ttttggtgga ttgactggca acaagggaca
     1741 caaggtatgc tagatccact ttggctttta aaccattatc actatcagga tagttgtaaa
     1801 aatgcagaag gtggtttgat tttatcaaga tatgcaggtc ctggtagtca ccgctaccct
     1861 gttggttttt caggggatac tattattagt tggaattcct taaggtttca accctatttt
     1921 acagcgacag catctaatat cggttatagt tggtggagtc atgatatcgg tggacatatg
     1981 ttgggggatt atgacgaaga gctacaaact agatggctac agtttggtgt ttttagtccg
     2041 attactcgat tacatagttc tagaagtcct tttaatagta aagaaccttg gtttttttca
     2101 gaaacaacat ctaagattat gaagaaatac cttcgtttga gacatcagat gattccttat
     2161 ctatatacca tgaatgtaaa gacacatgag gaaggtgccc cattaatcag tccaatctat
     2221 tatttctatc cagagaatga tgagagctat aatgttccaa accaatactt ttttggaaca
     2281 gaactgatgg tggctcccat tgtagaaaag atggatttga cattccaatc tgcaaaagta
     2341 gatgtatggt tccctgaagg tgaatggtat gacttctttt cagagaaaaa atacacaggt
     2401 ggtgtgaagt taagtgttta tagggacatc tcgactacgc ctgtgtttgc aaaaagtggt
     2461 gcaatcattc ccttggttgg ttctgagata ggtatgggtg ttgatttacc tgaggttgta
     2521 gattggtatg tattcccagg gaaacaacat tcttttgaaa tgcttgaaga tcaaaatggt
     2581 caaagatata aaacaagatt atcaatcgat tgggaaatgg gaatggtaga gttagcatta
     2641 caaggagatt ctagtatcgt tccaagcaat agaaaacata gaattcattt taaaggaacg
     2701 aatgtgtcta taattgaatt gccaaataag aatgatacag ctaaatttga atggaaagat
     2761 aataaaagga catctttaaa tgacgaagtt tttagactac taaagacagc ttctcttcca
     2821 tatgaattaa aagatagatt gttaaatcaa ttcatcaatg cgaaaaactc tcatgactta
     2881 atgaatatct tgcatcatca ggataaggaa ttgagagggc gtttgttgga aatgatattt
     2941 actagccaaa actaattgaa ataattagaa agtcatttgt ttagtgtata tgaagattct
     3001 gtatggtatt cgttatcact ggtcacattg aaaatgtgaa ataagcacct acaacataga
     3061 ctttacaaca cttcatgttt catatcatgg ggtgtttttc tatttaagtc aaagccaatc
     3121 aaaaaatctc actattcatc aggttaaagg aaacctaatc atagtgagat tgtgaattat
     3181 agtagagttt gtatcgcctc ttcaatttgt tttgggtctg tttttgaaga aaagcgttca
     3241 aagacttgcc catcacgacc aatgagaaac ttagcgaaat tccattcgat tcgttttcct
     3301 agtgggccag atttatggtc ttttaaccag acatagagag ggtctgcttc cttaccgttg
     3361 accttaatct tggcaaaacg tgggaaggtg gtttgaaaat gtaggctaca gaaggtgttg
     3421 atttcctctg cgctgccggg tgcttgtccc ataaactgat tgcaagggaa atccaatatt
     3481 tcgaagccct gatcttgata gcgttcatag agttcttgaa gtctctggta ctggggcgtt
     3541 aaaccacatc cagtagcagt gttgacaatc aagagaactt tgccacgata gctatccaag
     3601 ggagttgctt ggttgttttg gttcaaaacg gaaaaatcat aaagtgaagt catgagggcc
     3661 tcttttcttt ttctacattg taacagtttt tacttttttc gtcgagtcgg atggaagata
     3721 ttgttgaaga ataagccctc aatatgatag aaaccgttta caaataactt taaaaatggt
     3781 atgatttaat taaaccggtt tactttgttg ctataaatct ctaatttgag atttagttcc
     3841 tattgatttt acaatatgtt tattggagtg tatacatgca aacaaaaaca aagaagctca
     3901 ttgtgagttt gtcttcactt gttttatcag gatttttatt aaaccattat atgacagttg
     3961 gagcggaaga aacgactacg aataccattc agcaaagcca gaaggaagtt cagtatcagc
     4021 aaagggatac aaaaaattta gttgaaaatg gtgattttgg tcagacggag gacggaagca
     4081 gtccgtggac aggaagcaaa gctcaggggt ggtcagcttg ggtagaccag aagaatagtt
     4141 ctgcagatgc ctcaactcga gtcattgagg ctaaggatgg ggctatcact atctcaagcc
     4201 ctgagaaatt aagggcagcg gttcaccgta tggttcctat tgaagctaag aaaaagtata
     4261 aactgcgttt caagattaaa acagataata aagtcgggat tgccaaggtt cgtatcattg
     4321 aggaaagtgg taaggacaag cgattgtgga attctgcaac gacgtcagga acaaaggact
     4381 ggcagaccat tgaagcagac tatagcccga ctttagatgt tgataaaatc aagctggagt
     4441 tattctatga aacaggaact gggactgttt cctttaagga tattgagctg gtagaggtag
     4501 cagcccagct ttctgaggat tctcaaacag ataaacagct tgaggaaaag attgatttac
     4561 caattggaaa aaaacatgtt ttttctcttg cggactatac ttataaggta gaaaatcctg
     4621 acgttgcttc agtcaaaaat ggaattttag aacctcttaa ggaagggaca accaatgtca
     4681 ttgtcagtaa agatggcaag gaagtgaaaa agattccttt gaagattcta gcctctgtta
     4741 aggatgcata cacagcccgt ttggatgact ggaatggcat catcgctggg aatcaatact
     4801 atgattctaa aaatgaacag atggccaaat taaaccagga attggaagga aaggtagctg
     4861 atagcctatc tagtatttca agtcaggcgg accgcaccta tttgtgggaa aaattttcaa
     4921 attataaaat gtctgcaaat ctgactgcca cttatcggaa attggaggag atggccaagc
     4981 aagtgaccaa tccttcttct cgttattatc aagatgaaac tgtcgttcga acagtcaggg
     5041 attccatgga atggatgcat aaacatgtct acaatcgtga aaagagcatt gttgggaact
     5101 ggtgggatta tgaaatcggt acacctcgtg ccatcaacaa taccttgtct ctgatgaaag
     5161 aatacttctc tgatgaggaa attaaaaaat atacagatgt gattgaaaaa tttgtaccag
     5221 atcccgaaca tttccgaaag acgactgata acccatttaa ggctctaggt ggaaacttag
     5281 ttgatatggg aagagtaaaa gtaatagctg gtttactgcg taaggatgat caagaaattt
     5341 cttctaccat tcgctcgatt gagcaagtgt tcaagttggt agaccaaggt gaaggttttt
     5401 atcaagatgg atcctatatc gaccacacca atgttgccta tacgggtgct tatgggaatg
     5461 ttttgattga tggcctgtct caactgttgc cagtcattca aaagaccaag aatccaatcg
     5521 ataaagataa aatgcaaacc atgtaccact ggattgataa atcgtttgct cctttgctgg
     5581 tgaatggaga gctgatggat atgagtcgtg gacgctcgat cagtcgtgca aatagcgagg
     5641 ggcacgtggc cgcagtagaa gtactaagag ggattcaccg aatagcggat atgtctgaag
     5701 gagaaaccaa acaacgtttg cagagtcttg tgaagaccat tgttcaatcg gatagttatt
     5761 atgatgtctt taagaatttg aagacttata aggatatcag tttgatgcaa tccttgttaa
     5821 gtgatgcagg agtcgcaagt gttccaagaa caagttacct atctgccttt aacaagatgg
     5881 ataaaacagc catgtacaat gcagagaaag ggtttggatt tggcttgtca ctcttttcca
     5941 gtcgtacctt gaattacgaa cacatgaaca aggaaaataa acgtggttgg tatacgagtg
     6001 atgggatgtt ctatctttac aatggcgatt tgagtcacta tagcgatggc tactggccaa
     6061 cagttaatcc atataagatg cctggtacaa cagagacgga tgctaagaga gcggatagcg
     6121 atacaggtaa agttttaccg tctgctttcg ttggaacgag caaactagat gatgccaatg
     6181 cgacagcaac catggatttc accaactgga atcaaacatt gactgctcat aagagctggt
     6241 ttatgctaaa ggataagatt gcctttttag gaagcaatat ccaaaacact tcaacagata
     6301 ctgctgcaac tacaatcgac cagagaaaac tggaatcaag taatccatat aaagtctatg
     6361 tcaatgataa agaagcctcc cttacagaac aagaaaagga ttatcctgaa acccaaagtg
     6421 tctttttaga atcgtccgat tcgaaaaaga atattggtta ctttttcttt aagaagagtt
     6481 caatcagcat gagtaaggct ttgcaaaagg gagcctggaa ggatatcaat gaaggacagt
     6541 cagacaagga agttgaaaat gaatttctta cgattagtca ggctcataag caaaatggag
     6601 attcttatgg ctatatgctc attcctaacg tggatcgtgc caccttcaat caaatgataa
     6661 aagagttaga aagtagcctc atcgaaaata acgaaaccct tcagtctgtt tatgatgcca
     6721 aacaaggagt ttggggcatt gtgaaatatg atgattctgt ctctactatt tccaaccaat
     6781 tccaagtttt gaaacgtgga gtctatacca ttcgaaaaga aggggatgaa tataagattg
     6841 cctactataa tcctgaaacc caggaatcag ctccagatca ggaagtcttt aaaaagctag
     6901 agcaagcagc tcagccacaa gtacagaatt caaaagaaaa ggaaaaatct gaagaggaaa
     6961 agaaccattc ggatcaaaag aatctccctc agacaggaga aggtcagtca atcttggcaa
     7021 gtctagggtt cttgctactt ggggcgtttt atctattccg tagaggaaag aacaactaat
     7081 ttgttcatca tctagatgaa ttacatgaaa ctgtggaaaa tagaaaaaat ccgaagaagg
     7141 ccaaatatct tcggattttt cttttaaaaa aggataagtt gatacttatc ctttttactt
     7201 cattatgtga ctatagcgtt ttgaaacta
//
LOCUS       DAYYQN010000080         7205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYYQN010000080 DAYYQN010000000
VERSION     DAYYQN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.081"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            141..1010
                     /gene="rfbA"
                     /locus_tag="ACHBRQ_001856"
     CDS             141..1010
                     /gene="rfbA"
                     /locus_tag="ACHBRQ_001856"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9593469.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFIGDDSV
                     ALILGDNIYHGPGLSKMLQKAAKKEKGATVFGYHVKDPERFGVVEFDENMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            1011..1607
                     /locus_tag="ACHBRQ_001857"
     CDS             1011..1607
                     /locus_tag="ACHBRQ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFM9593470.1"
                     /translation="MTDNFFGKMLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPASFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            1617..2666
                     /gene="rfbB"
                     /locus_tag="ACHBRQ_001858"
     CDS             1617..2666
                     /gene="rfbB"
                     /locus_tag="ACHBRQ_001858"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9593471.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKTEKE
                     AVEANYAKTQEIITV"
     gene            2732..3583
                     /gene="rfbD"
                     /locus_tag="ACHBRQ_001859"
     CDS             2732..3583
                     /gene="rfbD"
                     /locus_tag="ACHBRQ_001859"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9593472.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNIAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            3646..4729
                     /gene="glf"
                     /locus_tag="ACHBRQ_001860"
                     /pseudo
     CDS             3646..4729
                     /gene="glf"
                     /locus_tag="ACHBRQ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709511.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5050..7032
                     /locus_tag="ACHBRQ_001861"
     CDS             5050..7032
                     /locus_tag="ACHBRQ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9593473.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         2362 a         1230 c         1564 g         2049 t
ORIGIN      
        1 gatttaatat taataatttt ttaaagtgaa taaaatcaaa gcaataaaag attaaaaaat
       61 aaattgctat ctattatgtt tttctcaaac taagtaaaac ataacttatg aaaacaaatt
      121 taaaaaggag ggactatagt atgaaaggta ttattcttgc aggtggttcg gggacacgat
      181 tatatccttt gactcgggct gcatcaaaac aacttatgcc ggtttatgat aaacccatga
      241 tttactatcc actttcaaca ttgatgttgg ctgggattag ggatattttg ataatctcaa
      301 ctcctcaaga tttgcctcgt tttaaagagc ttcttcaaga cggatctgag tttggtatta
      361 aactttctta tgcagagcaa ccaagtccag atggtttggc acaagccttt atcattgggg
      421 aagagtttat tggtgatgat agtgttgctt tgatcttggg ggacaatatc tatcacggtc
      481 ctggtttgag caaaatgctt caaaaagcag ccaagaaaga gaaaggtgcg actgtctttg
      541 gctaccatgt caaggatcca gaacgctttg gtgttgtaga atttgatgaa aatatgaatg
      601 ctatctccat cgaagaaaag ccagaatacc ctcgttcaaa ctatgcagtg acaggactct
      661 atttctatga taatgatgtt gtcgaaattg ccaagagtat caaaccaagt cctcgcggag
      721 aattagaaat cacagatgtc aacaaggctt acttggatcg tggagactta tccgttgagc
      781 ttatgggacg tggttttgct tggttggata ctggcactca tgaaagttta ctagaggctt
      841 cgcagtacat cgaaacagtc caacggatgc aaaatgttca ggtagcaaac ttggaagaaa
      901 ttgcctatcg catgggttat atcagtcgtg aagatgtatt gaccttagcc caaccactta
      961 agaaaaatga atacggacag tatctgctcc gtttgattgg agaagcatag atgacagata
     1021 attttttcgg taagatgctt gcggcacgca aggttgaagc tattccaggc atgttggagt
     1081 ttgatatccc cgttcatgga gataatcgtg gctggtttaa agaaaatttc caaaaggaaa
     1141 aaatgcttcc acttggattt ccagcgtctt tctttgcagc aggaaaattg caaaacaatg
     1201 tatccttctc acgtaaaaat gtccttcgag gcctccacgc agagccttgg gataagtaca
     1261 tctctgtagc agatggaggg aaagttctgg gttcttgggt tgatctacgc gagggtgaaa
     1321 cctttgggaa tacctatcag acagtgattg atgcgagtaa gggaatcttt gttcctcgag
     1381 gcgtagctaa tggcttccaa gttttatcag atacagtgtc atatagctat ctggtcaatg
     1441 attactgggc tcttgaactc aaacccaagt atgcctttgt gaactacgct gatccaagcc
     1501 ttggtattga atgggaaaat attgcagaag cagaggtttc agaagcagat aaacatcatc
     1561 ccctacttaa ggacgtgaag cctttgaaaa aagaagattt ggaataagga aagaatatga
     1621 ctgaatacaa aaatattatc gtgacaggtg gagctggctt tatcggttct aactttgtcc
     1681 attatgttta cgagaacttt ccagatgttc acgtgacagt cctagataag ttgacttatg
     1741 ctggaaaccg cgcgaatatt gaggaaattt taggtaatcg tgttgagtta gttgttggtg
     1801 acattgctga tgcggagttg gtagacaagt tggctgctca agcagatgct atcgttcatt
     1861 atgcagcgga aagccacaat gataattcgc tcaatgatcc atcgccattt attcatacta
     1921 acttcatcgg aacctatact cttttagaag ctgctcgtaa gtatgatatt cgcttccacc
     1981 atgtatcgac agatgaagtt tatggagatc tccctttacg cgaagatttg ccaggtcatg
     2041 gagaagggcc gggtgagaaa tttacggctg aaaccaagta caatccaagc tcgccttact
     2101 catcaaccaa ggcagcctca gatttgattg tcaaagcctg ggtgcgttct tttggagtca
     2161 aggcaacgat ttccaactgt tcaaataact acggtcctta tcaacatatc gaaaaattca
     2221 tcccacgtca gattactaac atcctaagtg gtatcaagcc aaaactttac ggtgaaggta
     2281 aaaacgttcg tgactggatt cataccaatg accattcttc aggagtttgg acaatcttga
     2341 caaaagggca aatcggtgaa acctacttga ttggggctga tggtgagaag aacaataagg
     2401 aagttttgga acttatcctt aaggaaatgg gacaagctgc ggatgcctat gatcatgtga
     2461 ctgaccgtgc aggacatgac cttcgctatg cgattgatgc tagcaagctc cgtgatgagt
     2521 tggggtggaa acctgaattt accaactttg aagctgggct caaggcaaca atcaagtggt
     2581 atacagataa ccaagaatgg tggaaaacag aaaaagaagc tgttgaagcc aattatgcta
     2641 agactcagga gattattaca gtataaaaag caggaaatag ctgcttttta ttgctatatt
     2701 gggaagagtt acatattaga aaggtctaga gatgatttta attacagggg caaatggcca
     2761 attaggaacg gaacttcgct atttattgga tgaacgtaat gaagaatacg tggcagtaga
     2821 tgtggctaag atggacatta ccaatgaaga aatggttgag aaagtttttg aagaggtgaa
     2881 accgacttta gtctaccatt gtgcagccta caccgctgtt gatgcagcag aggatgaagg
     2941 aaaagagttg gacttcgcca tcaatgtgac ggggacaaaa aatatcgcaa aagcatctga
     3001 aaagcatggt gcaactctag tttatatttc tacggactat gtctttgacg gtaagaaacc
     3061 agttggacaa gagtgggaag ttgatgaccg accagatcca cagacagaat atggacgcac
     3121 taagcgtatg ggggaagagt tagttgagaa gcatgtgtct aatttctata ttatccgtac
     3181 tgcctgggta tttggaaatt atggcaaaaa cttcgttttt accatgcaaa atcttgcgaa
     3241 aactcataag actttaacag ttgtaaatga ccagtacggt cgtccgactt ggactcgtac
     3301 cttggctgag ttcatgacct acctagctga aaatcgtaag gaatttggtt attatcattt
     3361 gtcaaatgat gcgacagaag atacaacatg gtatgatttt gcagttgaaa ttttgaaaga
     3421 tacagatgtc gaagtcaagc cagtagattc cagtcaattt ccagccaaag ctaaacgtcc
     3481 gctaaactca acgatgagcc tggccaaagc caaagctact ggatttgtta ttccaacttg
     3541 gcaagatgca ttgcaagaat tttacaaaca agaagtgaga taagtagtag aatgattttc
     3601 tagtctaata aaagaggcag agaatgaact ccaaaggagc ttaagatgta cgattatctt
     3661 gttgttggtg cagtctttgc ccatgaagca gtcttaaaag gaaaaaaagt aaaagttatt
     3721 gaaaaatgaa atcatatcgc gggtaatatc tatactcgtg aagaggaagg aattcaagtt
     3781 catcaatatg gtgctcatat cttccatact tctgataagg agatctggga ttatgtaaat
     3841 cagtttgcag actttaaccg ttataccaat tctcctgttg caaactataa gggtgagatt
     3901 tataaccttc cttttaatat gaataccttc aataaactct ggggagttgt gacgccagca
     3961 gaagcacaag ctaagattga tgaacaacgt gctattttaa atggtaaaac tcctgaaaat
     4021 ttggaagaac aggcgatttc tcttgtaggt acagacatct acgaaaaatt aatcaaagac
     4081 tatacagaga aacagtgggg caaaccaact actgaacttc cagcctttat tattgtttgc
     4141 cagtacgcct gacctatgat aacaactatt ttaacgatac ctatcaaggg attccaattg
     4201 gtggatacac tcaaatagtt gaaaaatgtt ggatcatgaa aatattgatg tagaaacaaa
     4261 tgttgatttc tttgtgaata aagagcaata tctgaaagat tttcctaaga ttgtctttac
     4321 tggtatgatt gatgaattct ttgactataa gttgggcgaa ctagagtacc gtagtcttcg
     4381 ttttgaaaat gagaccttgg atatggaaaa ttaccaagga aatgcagttg tgaactatac
     4441 ggatgcagaa atcccatata ctcgcattat tgaacacaaa cattttgagt ttgggagtca
     4501 agcaaagact atcattacta aagaacattc taaaacatgg gaaaaaggtg atgagcctta
     4561 ttatccagtt aataatgatc gtaataatca tttgtataaa tcgtataaaa aacttgctga
     4621 tgagcaaggg aatgttatct ttggtggtcg cttaggacac tatcgttatt acgatatgca
     4681 ccaagtaatt ggagcagctt tgcagtgcgt gagaaatgag ttagattaat actcaatgaa
     4741 aatcaaagag caaactagga agctagccac aggttgctca aaatactgtt ttgaggttgc
     4801 agatggaagc tgacgcggtt tgaagagatt ttcgaagagt ataaacaagt aaaactgact
     4861 accagttatt atttagaaat agtattaaaa attccttgac tatgtgatat agttgaggga
     4921 tttttaaatg atattcatat tttttgcaaa gatgttgttt gaaaaataat tttcaaaaat
     4981 tctgaaaatt ctgttgacat ctttctgaaa agagtttata atggagagaa agttttaaag
     5041 gagaaaatga tgaaaagttc aaaactattt gcccttgcgg gcgtgacatt attggcggcg
     5101 actactttag ctgcatgctc tggatcaggt tcaagcacta aaggtgagaa gacattctca
     5161 tacatttatg agacagaccc tgataacctc aactatttga caactgctaa ggctgcgaca
     5221 gcaaatatta ccagtaacgt ggttgatggt ttgctagaaa atgatcgcta cgggaacttt
     5281 gtgccgtcta tggctgagga ttggtctgta tccaaggatg gattgactta cacttatact
     5341 atccgtaagg atgcaaaatg gtatacttct gaaggtgaag aatacgcggc agtcaaagct
     5401 caagactttg taacaggact aaaatatgct gctgataaaa aatcagatgc tctttacctt
     5461 gttcaagaat caatcaaagg gttggatgcc tatgtaaaag gggaaatcaa agatttctca
     5521 caagtaggaa ttaaggctct ggatgaacag acagttcagt acactttgaa caaaccagaa
     5581 agcttttgga attctaagac aaccatgggt gtgcttgcgc cagttaatga agagtttttg
     5641 aattcaaaag gggatgattt tgccaaagct acggatccaa gtagtctctt gtataacggt
     5701 ccttatttgt tgaaatccat tgtgaccaaa tcttctgttg aatttgcgaa aaatccgaac
     5761 tactgggata aggacaatgt gcatattgac aaagttaaat tgtcattctg ggatggtcaa
     5821 gataccagca aacctgcaga aaactttaaa gatggtagcc ttacagcagc tcgtctctat
     5881 ccaacaagtg caagtttcgc agagcttgag aagagtatga aggacaatat tgtctatact
     5941 caacaagact ctattacgta tttagttggt acaaatattg accgtcagtc ctataaatac
     6001 acatctaaga ccagcgacga acaaaaggca tcgactaaaa aggctctctt aaacaaggat
     6061 ttccgtcagg ctattgcctt tggttttgat cgtacagcct atgcctctca gttgaatgga
     6121 caaactggag caagcaaaat cttacgtaat atctttgttc caccaacatt tgttcaagca
     6181 gatggtaaaa actttggcga tatggtcaaa gagaaattgg tcacttatgg ggatgaatgg
     6241 aaggatgtta atcttgcaga ttctcaggat ggtctttaca atccagaaaa agccaaggct
     6301 gaatttgcta aagctaaatc agccttacaa gcagaaggtg tgactttccc aattcatttg
     6361 gatatgccag ttgaccagac agcaactaca aaagttcagc gcgtccaatc tatgaaacaa
     6421 tccttggaag caactttagg agctgataat gtcattattg atatccaaca actacaaaaa
     6481 gacgaagtaa acaatattac atattttgct gaaaatgctg ctggcgaaga ctgggattta
     6541 tcagataatg tcggttgggg tccagacttt gccgatccat caacctacct tgatatcatc
     6601 aaaccatctg taggagaaag tactaaaaca tatttagggt ttgactcagg ggaagataat
     6661 gtagctgcta aaaaagtagg tctatatgac tacgaaaaat tggttactga ggctggtgat
     6721 gaggctacag atgttgctaa acgctatgat aaatacgctg cagcccaagc ttggttgaca
     6781 gatagtgctt tgattattcc aactacatct cgtacagggc gtccaatctt gtctaagatg
     6841 gtaccattta caataccatt tgcattgtca ggaaataaag gcacaagtga accagtcttg
     6901 tataaatact tggaacttca agacaaggca gtcactgtag atgaatacca aaaagctcag
     6961 gaaaaatgga tgaaagaaaa agaagagtct aataaaaagg ctcaagaaga tctcgcaaaa
     7021 catgtgaaat aactgttgca aaatataaga aaggatttag tatttctctt gaatgctgaa
     7081 tcctttttta catttgtaaa gaaagattct aaatgtactg acccccaaaa gttggagcct
     7141 ctttttgtca gaatagagaa aatttttgtt aattttactt gtttcctatt gctttctcag
     7201 ctatt
//
LOCUS       DAYYQN010000081         7193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYYQN010000081 DAYYQN010000000
VERSION     DAYYQN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.082"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..245
                     /locus_tag="ACHBRQ_001862"
     CDS             <1..245
                     /locus_tag="ACHBRQ_001862"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9593474.1"
                     /translation="RSTNHKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLSLPPL
                     ALQNEFADFVALVDKSEFACEIAIKVWRNSLKFSII"
     gene            275..3625
                     /locus_tag="ACHBRQ_001863"
     CDS             275..3625
                     /locus_tag="ACHBRQ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9593475.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRDIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRARMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3963..4187
                     /locus_tag="ACHBRQ_001864"
     CDS             3963..4187
                     /locus_tag="ACHBRQ_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593476.1"
                     /translation="MEADEVSSKHGFEVVDETDEVSSKHGFEVVDETDEVSSKHGFEV
                     VDETDEVSNHTYGKAMLTRIEEIFEEYKSS"
     gene            complement(4304..4774)
                     /locus_tag="ACHBRQ_001865"
     CDS             complement(4304..4774)
                     /locus_tag="ACHBRQ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9593477.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFTPPFFFEE"
     gene            complement(4791..7064)
                     /locus_tag="ACHBRQ_001866"
     CDS             complement(4791..7064)
                     /locus_tag="ACHBRQ_001866"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HFM9593478.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVSLPHKIELEKPQLNLVQPQGKAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFATLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNDRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2217 a         1218 c         1555 g         2203 t
ORIGIN      
        1 ccaggtcgac aaatcacaaa tttattatcc atgttttaag gaataataat tatagtcgag
       61 tgatatcagg aagtgctcag cctcagttac caattacaaa attaaaaaaa atacttctct
      121 cccttccccc actagccctc caaaatgagt tcgcagactt tgtagccctg gtcgacaaat
      181 cagaatttgc ttgtgagata gctataaaag tgtggagaaa tagcttgaaa tttagtataa
      241 tatagctaaa ctatttgttt aaagtgagaa aaaaatggga aattttagct ttcttttaaa
      301 aaatgacgaa tatgaatctt tttcaaaacc ttgcattgaa gctgagaata tgattgctac
      361 atcaactgtg gctactgcct ttatggcgcg tcgtgcttta gagcaggctg tccattggat
      421 atatagtcac gattcatatt tagaagctcc ctatcgtgct actctatctt ctttagtatg
      481 ggatgatgat tttagggata tcgtagattc tgaactccac aagcagatag ttctgttgat
      541 tcggtgggga aaccatgctg ctcatggtgg tgaaattaag gaacgagaag cgattttagc
      601 tttgcatcat ttgtatcagt ttgttaattt tatcgattat tgttacagca atgagtttgt
      661 ggagcgttat tttgatgaga agtgcttacc actttcagca aacatcaaat accgagaaac
      721 tccacaatct atgataaagt tacaagacag tttaccagaa ctgcctgatt ttcatgaaca
      781 gatggctgct cagtccgtag aagttcaaga gacttatact gaaaaacgtg aaactgcagc
      841 gcaacggcaa gatgtgcctt tccatattga tcaattatct gaggcagaga caagaaagct
      901 ctttattgat atcgatctcc gtttagcagg atggatattt gaagaaaact gtcgtgttga
      961 gatagccgtt gatggtctca agcacggttc aggaattggt tactgtgact atgtacttta
     1021 tggtaaaaat gggaaaattt tagcgattgt ggaggctaaa aaagcctctg tcaatccaga
     1081 agtaggggaa gtacaggtca aagaatatgc tgaagctttg gagaaacata tcggctatca
     1141 gccaatttgc tttattacaa atgggttgaa gcactatata cttgatggtc cgaaccgccg
     1201 ccagattgca ggcttttact ctcaagaaga attgcaatta gtgatggata gacgtcatct
     1261 tcaaaaaccg cttgaggata tttctagtaa aattagggac gatatttccg ggcgtcacta
     1321 ccaaaaacat gccattgcaa gcgtttgtga agctttctct aatcatcgta gacaggcact
     1381 tttggttatg gcaactgggg cggggaaaac tcgtacagca gtttctctag ttgatatctt
     1441 atcacgtcat aactgggtaa aaaacgttct cttcttagcc gatagaactt ccttggttaa
     1501 gcaagcctat gattcgttta gaaaattact cccagatctt tccgtttgta acttcttaga
     1561 agataaagaa ggagctcaat caagtcgcat ggtcttttca acttatccga ccatgattgg
     1621 agcgattagt ggtcaagaag aagtaaatca acgccctttc actgttgggc attttgacct
     1681 tatcataatt gacgaatctc accgttctat ttatcagaaa tacaagtcca tttttgatta
     1741 ttttgatgca aggattgtag gcttaacagc tactccgcgt caagatttag ataaaaacac
     1801 ctatggattc tttaatttgg agaatggggt tccaacatat gcatatgatt tggaagaggc
     1861 tgttaaagac ggatatttag tagcctatca ttctatcgaa accaaactga aactacctac
     1921 ggatggtcta cattatgatg atttgtccga agaagaaaag gaacattttg atagcaaatt
     1981 tgaagacgat agctgtgaaa aagatattga tgggagtgta tttaattcct ttattttcaa
     2041 taaaagtaca gtagaaattg ttttaaatga actcatgaca agaggaattc agacagcctc
     2101 gggtgatgaa attggtaaaa ctattatttt tgctaaaaat catgatcatg cggaatatat
     2161 cagagatatt tttaacaacc gctatcctga aaaagggagc gactatgctc aggtaattga
     2221 ttatagtatt aagcattatc agaccttgat tgatgatttt aaaattaagg agaagtatcc
     2281 tcaaattgcg atttctgtag atatgttaga tacaggtatt gatgtaccag aggttgttaa
     2341 tttagtcttc ttcaagaaag tacgctctaa aactaagttt tggcagatga ttggtcgagg
     2401 aacccgtctg tgtaaagatt tatttggacc tgagcaggat aaggaaaact tcttggtatt
     2461 tgattatggg gacaattttg attattttcg tgcagatcca agagatggag agggtcgtca
     2521 cattgtttcg ttgactcagc gtttatttaa tatcaaagtg gacttgattc gagaacttca
     2581 gggactccaa taccaagaag atcagtttgc gagagcatac cgtcagcagc ttgtctcgga
     2641 acttcaaggt cgtatagaga gcttaaatga gttggacttc agggctcgta tggttttaga
     2701 tacagtttat agctatagga aattggaaag ttggcagaat ctaactgctg ttacaagtga
     2761 aaccattcaa aaaaatcttt ctccgctttt atttgatgaa gataaagaag atgagatggc
     2821 gaggagattt gatttgtggt tgcttcatat tcagttgggg caactgacag ctaaatcttc
     2881 cactgttcat atttcccaag tgatgaagac ggctagagct ctttctgcta ttggcaatat
     2941 cccgcaggtt tttgagcagg ctgaaattat caggaaagta caggaacctg aattttggaa
     3001 agaagttaac ttgtctgatt tggaaaaaat tcgtcttgct attcgagatt tattacagtt
     3061 tttggataaa acagaccgta aaccctacta tgttaacttt gaagatcgta tactctccac
     3121 tgttcacgag accacagcat ttttgcaggt caacgatctt cggtcttaca atgaaaaagt
     3181 tgagcattat ttgaaaactc atctggatga ggagtccatt tctaagctat accataataa
     3241 aaagttgaca tctgatgata tgcttgcact tgaaaaattg ctctgggaaa aattaggtag
     3301 taaagcagac taccaaagtc attatgaaaa taaggcaatt ccgagattgg ttcgtgagat
     3361 tattggttta gatagagagt ctgccaatcg tattttttct aaatttttgt cggatgagaa
     3421 tcttaatgcc aggcagattt catttgtaaa attgattgta gactacattg tagaaaatgg
     3481 ttttttagag acgaaagtgt taacgcaaga gccgtttaaa tcttatggtt ctgttcaact
     3541 actcttccaa caccaactac cagtacttcg taatattgtt caaatcattg aacttatcaa
     3601 taatcgagct ggagaagcgg cttaaattct aaagtgattg ccatgctgag actcatttaa
     3661 aattaaaaag agtagaaatt tatgctatat atgagaagtt ttattaggaa gaatgtcatc
     3721 gttttcctag aatacagtat cagttgttaa gtggttgata aatttcaaag tagatacttg
     3781 taccacgatg tttgttgatc gagttattaa caaaagagct actttgattt taaagaaata
     3841 gaaaacaaaa agccgagcaa gaattcaatt gcaggagaaa atgaaataat actcaatgaa
     3901 aatcaaagag caaactagga aactagctgc aggctgctca aaacactgtt ttgaggttgc
     3961 agatggaagc tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg
     4021 aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agctcaaaac
     4081 atggttttga ggttgtagat gaaactgacg aagtcagtaa ccatacctac ggcaaggcga
     4141 tgttgacgcg gattgaagag attttcgaag agtataaatc ttcctaggat aaagcaaaac
     4201 gcatagtatc aagggttttc aacacttgat actatgcgtt ttctgatgtt aaagactttc
     4261 taccaggttt tttaaaagca taattgttag ttgtagtcat ttattattct tcaaagaaaa
     4321 atggtggggt gaattttttc agttcttcaa agcacttttg agcagtatct gcatcttcac
     4381 agatgataag acagacatca ttaccacaaa gggtagcgat agcgtcaggg aagctcaaag
     4441 tatcaatgat agaaccaaag gattgagcca gtccaggaag ggtttttagt aggacttggt
     4501 gttgaactgg gcgcatccag acaagggcgt cttccatgta gagttcgaga cgtttttccc
     4561 attttgagat ggaaccattg ttaagaacat aataagcgct atcttcttcg cggacttttg
     4621 ataggttcat atttttgatg tcgcgtgaga gggttgcctg ggttacttga atgtcgttct
     4681 tagcaagaag ggcttgcaac tcagcctgtg tatgaatctt gttttttgtg ataagagcgc
     4741 gtataagttg gtggcggtgt tctgatttat tcataataat gtaactcctt ttagcaaggt
     4801 aaggtaagca tggactgagc gaggtcgaca gtcaagtggt agtctgtatt gtcacggatg
     4861 gtgatttcaa agtcagtagt atagaggact aaacggagag tgtctccttc ttttagcttg
     4921 taaatagttg gctgcagttc aaattgaacg tccatccatt catctgcagt aatatcctct
     4981 actaacagta aatcatttct attttgtaaa ttaaggtaac cttttgtcac gactcgttgt
     5041 gcctctggtc taaatggcaa ttcacagaga ttttccaaca tgtgatagcg accgttgtca
     5101 atggttctag cacttaaaat agctggataa ggttgtaggt atttcttttg cccaaattct
     5161 agcagttggg cagataagag ccccttgttt gtactggatt tgatacgaag attgagctga
     5221 gcgcgaccgt ttaggtggag atctttagtc acaggaaggt taatagtaat ctgattagct
     5281 ttcccttgat agagctctgt attgaaggtt tggtatgtct taccatagcg ctcaaaatcc
     5341 ttatctgggt actggttttg aatagcttgc tcttcttgac caagtgagaa ggtttcacag
     5401 ttttcttgcc caccgaagtt atcaagtgat aaccaagtct gtggagctgt attgtcctgc
     5461 cagataacag taggaagttg aaagtctgtt tcctgtccta gtaatttctt ggtcaataag
     5521 gcatttatgg actcacggaa gtcaattgat tgccaattgt tcatgtaaac atgggcacca
     5581 ttatggaaaa agagatgctt gtgtatatga gtaggaagag catggaacat ctggtaaaca
     5641 tgaagtggtt tgacattcca atcctgagaa ccatgagtaa agacaacctc tgcctttact
     5701 ttatgggcat tgagcagata attgcggtca tgccaaaact gattgtagtc cccagttttt
     5761 cggtctagct gagctttcac tttttctaag tcagcttggt gagcttcatt gccacggata
     5821 tagtcgccag ctaagagatt acgagaatag gttaactcag caagggagtc aaagtcctca
     5881 cctggataac cacctgggct agtcaccaga ccgttttcac ggtagtagtt gtaccatgat
     5941 gaaattcctg cctcggcaat gataacttct aaaccatcga ctcctgtagt cgcaagacca
     6001 ttggacatgg tacctagata ggaaagtccc gttgtagcaa cttttccgtt tgaccaatca
     6061 gccttgactt gacgctggcg cgtgtgatca gtaaaggcac ggcaacggtc gttaagccaa
     6121 tcgatgacat ttttataagc ctcgatttgc tggtagtctc cattagtcat gaaacctgtc
     6181 gagtctttgg taccaacacc tgagacatag agagtggcaa agcctctcgg aaggaagtag
     6241 tcgtttagtg tatagctaga gttgatgtga gttagctttt cctcagcctc tgctacaagc
     6301 tcagctttac cttggggttg gacgagattt agttgaggtt tctctagctc aatcttgtga
     6361 ggaagagaaa cctcaagctc gccctccatc ttgtagagag ccttgtcact agccttatca
     6421 ttggttccct gatgataagg gctggctgtc atgatggcag ggatttttcc atcaaaacga
     6481 gggcgaataa tgctaacctt tactaggtct gatagccctt tttggtcagt atcgacacga
     6541 gactcaacgt aaacgacttc acgaatgaca tcctggttag aaaaagtagc caaactcttg
     6601 ccgttaaagt agtggtagtc attatcctcc ggaataagac catcactaac aagttggtcg
     6661 ataagagtat ttcctttttt ggtgcgagta ttgagtaact gatagagatt ttcaatcaag
     6721 tcaccatata taatgggaaa tccagtttct ttacgaaaaa cgtcactatc ttcgaagtca
     6781 accaaataag caaagcctaa aagttgaaaa gcaacagtat aaaaaatatc tgctgtcagt
     6841 tcatcttctg attgaaaaaa tgtcagcagg tctgtttttt tatcagctgc taggatggaa
     6901 agtgggtagt tggtgtcttg ataagtgaaa aagaaacgac gtaaaaaggt ttcaagtgag
     6961 tctttgtgat tggctgtatt ttgtaaatca aagccacatt tttttagttc agataagaca
     7021 ttttcttttg gaaaattgat ataactatat tgattaaaac gcatagaacc tccatataga
     7081 atgacagtta aggttattat atcaaaaaaa aagcagaaag ggaattgtta acttcaaaag
     7141 gaaataatcc aataaaaatg aataaagtac taaattcaat atagagaaca gag
//
LOCUS       DAYYQN010000082         6746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYYQN010000082 DAYYQN010000000
VERSION     DAYYQN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.083"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..324
                     /locus_tag="ACHBRQ_001867"
     CDS             <1..324
                     /locus_tag="ACHBRQ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9593479.1"
                     /translation="TNGELIAPMTYEEMMTSDFFEAWFQKFLLPTLTTPSVIIMDNAR
                     FHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLS
                     CSCFN"
     gene            385..1360
                     /locus_tag="ACHBRQ_001868"
                     /pseudo
     CDS             385..1360
                     /locus_tag="ACHBRQ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1537..2544
                     /gene="gap"
                     /locus_tag="ACHBRQ_001869"
     CDS             1537..2544
                     /gene="gap"
                     /locus_tag="ACHBRQ_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9593480.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2612..3487)
                     /locus_tag="ACHBRQ_001870"
     CDS             complement(2612..3487)
                     /locus_tag="ACHBRQ_001870"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9593481.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVRWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTF
                     TTLSNTFEKELKKNG"
     gene            3574..5769
                     /gene="pbp2a"
                     /locus_tag="ACHBRQ_001871"
     CDS             3574..5769
                     /gene="pbp2a"
                     /locus_tag="ACHBRQ_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HFM9593482.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5822..5974
                     /gene="rpmG"
                     /locus_tag="ACHBRQ_001872"
     CDS             5822..5974
                     /gene="rpmG"
                     /locus_tag="ACHBRQ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9593483.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5984..6160
                     /gene="secE"
                     /locus_tag="ACHBRQ_001873"
     CDS             5984..6160
                     /gene="secE"
                     /locus_tag="ACHBRQ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9593484.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6215..>6746
                     /gene="nusG"
                     /locus_tag="ACHBRQ_001874"
     CDS             6215..>6746
                     /gene="nusG"
                     /locus_tag="ACHBRQ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9593485.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2098 a         1181 c         1457 g         2010 t
ORIGIN      
        1 acaaatggtg agttaatcgc tccaatgact tacgaagaga tgatgacgag cgactttttt
       61 gaagcttggt ttcagaagtt tctcttacca acattaacca caccatcggt tattattatg
      121 gataatgcaa gattccatag aatgggtaag ttagaacttt tatgcgagga gtttgggcat
      181 aaacttttac ctcttcctcc ctactcacct gagtacaatc ctattgagaa aacatgggct
      241 catatcaaaa agcacctcaa aaaggtatta ccaagttgca atacctttta tgaggctctt
      301 ttgtcttgtt cttgtttcaa ttgactatag ttcacggata cagttgggaa agaagctatt
      361 aaatatttgg agagtccttt tgggatgaac atgtcaaata ttactataga tgggcaagaa
      421 aagatgggca agaaatggat ctgagtcaaa cgatgcttct tatgctcaag tagtgagtct
      481 ttatgatgat acaagtatct ctgttagtaa taatgagacg gacaaagtgt tgactggatc
      541 cctctatact gaaacaaatg aacagggatt aacgattcca agcagtttac taaaaaattg
      601 gaatgaacag acaggaaaaa atttgacaac taatgatctt attggcaaat cagtctcagc
      661 cagcattgta gaaagtgctg ccgaaactag taagattgct caatttcaaa cgaagattgt
      721 acgtgtaatc aatgatgaag atgacatgga ggacagcaac agtttcatgc tgtctcatca
      781 aatggaaacg attttgaaag aggctggatt aacgaaagct gtatcttatt ttatcttgga
      841 actcaaagat ccatcacaga caaaagtagt aacagaagaa ttacagaaaa ataagaagta
      901 tactgtgctt tctcaacaga gagttcttga tattgtgatt acctttattc gtgttattca
      961 gggattattg attgtgcttt catcacaagc tattgtggta gcggcggtta tgattggtat
     1021 cattatttac atcaatatca tgcaacgttc caaggaaata ggtgtcatga aagcagttgg
     1081 ttatcagaat cgtggtgtca aaggaatttt tatttacgag gctatctgga ttgtaggcat
     1141 cgccttgctg ctggcatttt tggttgcaca aggggtggga agtttggcga atgcgattgt
     1201 aagtcacttt tacccatcca tcactaaggt ttttgaatta aatcttttat ctgttttagg
     1261 aactctagtt ttcgctctat tacttggtta tgtctcagcc tacttcccgg cgcgtaagat
     1321 tagtaaaatg gatcctgtag aatcgttacg ctatgaataa taagggattc cttgatttac
     1381 agtcttaaaa agaaactcac atagaaaaac aatggccata cttgcaattc aactcaaata
     1441 gttgtataat aggtttgttg aaaggtgatt tgtagtgatt caagttactc ttttcacaat
     1501 aaaaatttca tgattttcat aaggaggaaa tcactaatgg tagttaaagt tggtattaac
     1561 ggtttcggac gtatcggtcg tcttgctttc cgtcgtatcc aaaacgtaga aggtgttgaa
     1621 gttacacgca tcaacgacct tacagatcca gttatgcttg cacacttgtt gaaatacgac
     1681 acaactcaag gtcgtttcga cggtactgtt gaagttaaag aaggtggatt cgaagttaac
     1741 ggtaaattcg ttaaagtttc tgctgaacgt gatccagagc aaatcgactg ggctactgac
     1801 ggtgtagaaa tcgttcttga agctactggt ttctttgcta agaaagaagc agctgaaaaa
     1861 caccttaaag gtggagctaa aaaagttgtt atcactgctc ctggtggaaa cgacgttaaa
     1921 acagttgtat tcaacactaa ccacgacgtt cttgacggta ctgaaacagt tatctcaggt
     1981 gcttcatgta ctacaaactg cttggctcca atggctaaag ctcttcaaga taactttggt
     2041 gttgttgaag gattgatgac tactatccac gcttacactg gtgaccaaat gatccttgac
     2101 ggaccacacc gtggtggtga ccttcgccgt gctcgcgctg gtgctgcaaa catcgttcct
     2161 aactcaactg gtgctgcaaa agctatcggt cttgtaatcc cagaattgaa tggtaaactt
     2221 gatggatctg cacaacgcgt tccaactcca actggatcag ttactgaatt ggtagcagtt
     2281 cttgaaaaga acgttactgt tgatgaagtg aacgcagcta tgaaagcagc ttcaaacgaa
     2341 tcatacggtt acacagaaga tccaatcgta tcttcagata tcgtaggtat gtcttacggt
     2401 tcattgtttg acgcaactca aactaaagtt cttgacgttg acggtaaaca attggttaaa
     2461 gttgtatcat ggtacgacaa cgaaatgtca tacactgcac aacttgttcg tactcttgaa
     2521 tacttcgcaa agattgctaa ataattcttg agttgataga aagcaaggct ttgtggtctt
     2581 gcttttttat atggaaaaat ggatgacacg atcatccatt cttttttaat tctttttcaa
     2641 atgtatttga aagggtagtg aaagttagcc tctctaaagt aagtgggtgg gtaaaggaaa
     2701 gtcggaaggc atgaagcata agccggcttg tctttgattt actattatag agagggtctc
     2761 ccaggatagg aagattatga tgcaaaaggt gcacacgaat ctgatgggtt cgccctgtct
     2821 ttagcttgca atgagccaag gaagtcttgt ttgagaattg ctttaatctg cttacatgcg
     2881 tttcagcata tttcccattt tttgcatcaa ctattctttt tctacgatca tggcgatcac
     2941 gtccaatttt gtctctgaaa acaagttctt ttctgttgat atttccatca actagagccc
     3001 aatattctct agaaatctct tttttctcca ataagcgatt gagaatgggc aggataaaag
     3061 gatttttggc aaagagaact aagccactgg tttccatgtc cagacgatga acgacatagc
     3121 aggtttggcc aacataggta ctgacatggt taagaagggc aatttcgttt ggttgattac
     3181 catgcgtttt catcccctct ggtttgttta caataatcaa gtgttgatct tgataaactt
     3241 cctgcactaa gtctgggttg ccccaaggga tcgtcttttg gggataatct tcctcgtcaa
     3301 aagtcaactg gcaaacatct ccaggattta cgatttcgtt ccagcggact tcttcttgat
     3361 ttatcaaaat atgtttcttg attctcaaaa aatgacggat ttttctaggg atgaggagtt
     3421 gttcctcaag taattgcttt accgtcattt gaggtagaga ggcgggtaat gtaaatgtga
     3481 attgcataca gatattgtaa caaaaaaagc cctatttgga taggaaatag ctaaattctt
     3541 gtcttcctat gatgaagatg ataaaataaa cgcatgaaat tagataaatt atttgagaaa
     3601 tttctttctc tttttaaaaa agaaacaagt gaactagagg actctgattc tactatctta
     3661 cgtcgctctc gtagtgatcg aaaaaaatta gcccaagtag gtccgattcg aaaattctgg
     3721 cgtcgttatc atctaacaaa gattatcctt atactaggtt tgagtgcagg cttgctagtt
     3781 ggaatctatt tgtttgctgt agccaagtcg accaatgtca atgatttgca aaatgccttg
     3841 aaaactcgga ctcttatttt tgaccgtgaa gaaaaagagg ctggtgcctt gtctggtcaa
     3901 aagggaacct atgttgagct gactgacatc agtaaaaact tgcagaatgc tgttattgcg
     3961 acagaagacc gttctttcta taaaaatgac gggattaact atggccgttt cttcttggct
     4021 attgtcactg ctggacgttc aggtggtggc tctaccatta cccaacagct ggctaaaaac
     4081 gcctatttat cgcaggatca aactgttgag agaaaagcga aagaattttt ccttgcctta
     4141 gaattaagca aaaaatatag taaggagcaa attctaacca tgtaccttaa caacgcttat
     4201 tttggaaatg gtgtgtgggg tgtagaagat gcgagtaaga aatactttgg agtttctgca
     4261 tcagaagtga gtctggatca agctgcgact ctggcaggga tgctcaaggg gccggaactg
     4321 tataatccct tgaattccgt agaagattct actaatcggc gcgatactgt cttgcagaat
     4381 atggttgcag caggatatat tgataaaaac caagaaaccg aagctgctga agttgatatg
     4441 acttcgcaat tgcacgataa gtatgaagga aaaatctcag attaccgtta cccctcttat
     4501 tttgatgcgg tggttaatga agctgtttcc aagtataatc taacagagga agagattgtc
     4561 aataatggct accgcattta cacagagctg gaccaaaact accaagcaaa tatgcagatt
     4621 gtttatgaaa acacatcgct atttccgagg gcagaggatg gaacgtttgc tcaatcagga
     4681 agtgtagctc tcgaaccgaa aacaggggga gttcgtggag ttgtcggtca agttgctgac
     4741 aatgataaaa ctggattccg gaatttcaac tatgcaaccc aatcaaagcg tagtcctggt
     4801 tctacaatta agcctttagt tgtttataca ccagcagttg aagcaggctg ggctttgaat
     4861 aagcagttgg ataaccatac catgcagtat gatagctata aggttgataa ctatgcaggg
     4921 atcaaaacaa gtcgagaagt tcctatgtat caatccttgg cagaatcgct taatctacct
     4981 gctgttgcca ctgttaatga tttgggtgtt gacaaggctt ttgaggcagg cgaaaaattc
     5041 ggactcaaca tggaaaaggt cgaccgtgtt cttggtgtcg ccttgggaag cggtgttgaa
     5101 accaaccctc ttcaaatggc tcaagcatac gctgcctttg caaatgaagg tttaatgcct
     5161 gaagctcatt ttattagtag aattgaaaat gctagtggac aagttattgc gagtcataaa
     5221 aattcacaaa aacgggtgat tgataagtct gtagctgaca agatgaccag tatgatgttg
     5281 gggactttca ccaacggtac cggtattagt tcatcgcctg cagactatgt catggcaggg
     5341 aaaactggaa caactgaagc agttttcaat ccggagtaca caagtgacca gtgggtaatt
     5401 ggttatactc cggatgtagt gattagccac tggcttggct ttccgaccac tgatgaaaat
     5461 cactatctag ctggctctac ttcaaacggt gcagctcatg tctttagaaa cattgccaat
     5521 accattttac cttatacgcc aggaagtacc tttacggttg aaaatgctta taagcaaaat
     5581 ggaattgcac cagccaatac aaaaagacaa gtacaaacca atgataatag ccagacagat
     5641 gataatttgt ctgatattcg agggcgtgcg caaagtctag tagatgaggc tagccgggct
     5701 atctcagatg cgaagattaa ggaaaaggct caaacaatat gggattcgat agtcaatcta
     5761 tttcgctaag atgcttgtca aagcctagct ttcttgttat aatggataag atggaggcgt
     5821 tatggcacta aaaaaagcaa gcctagcttg tgcggtttgt ggttcgagaa actattcaat
     5881 caagatcagc ggaaacccca agcctacacg actagaagta aataaatttt gtaagcattg
     5941 tggcaagtac actacacaca gagaaacgag ataggagaga gcgatgcgtt ttattggaga
     6001 tatttttaga cttcttaaag acacaacatg gccaactcgc aaggaaagct ggagagattt
     6061 tcgttctatc atggaataca cagctttctt tgtagtaatt atttacattt ttgaccagtt
     6121 gattgtttca ggtttgattc gatttattaa cattttttag aagattagtg gagttaatta
     6181 cactagaaat cttctattta tgaaaggaaa tatcatggat agttttgata aaggatggtt
     6241 tgttttacaa acttattctg gttatgaaaa taaggtgaaa gaaaatctat tacaacgtgc
     6301 acaaacctac aatatgttgg ataatattct acgcgttgaa attccaacac aaacagtgca
     6361 agttgaaaaa aatggaaaga gaaaagaagt agaagaaaat cgctttccag gttatgttct
     6421 tgtagaaatg gtcatgacag atgaagcttg gtttgttgtt cgaaacacac caaatgttac
     6481 aggatttgtc ggatcacacg ggaacagatc aaaaccaact ccattattgg aacaagaaat
     6541 tcgtgacatt ttggtatcta tgggacaaac tgttcaagaa tttgatttcg atgttgagat
     6601 tggtcaaact gtacgtatta ttgatggtgc ttttgcagac tacactggta agattacaga
     6661 aattgataat aacaaagtga aaatgattat ctctatgttt ggtaatgaca cagttgcaga
     6721 agtaaaccta aaccaaatcg cagaat
//
LOCUS       DAYYQN010000083         6088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYYQN010000083 DAYYQN010000000
VERSION     DAYYQN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.084"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            19..1869
                     /locus_tag="ACHBRQ_001875"
     CDS             19..1869
                     /locus_tag="ACHBRQ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9593486.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(1955..2167)
                     /locus_tag="ACHBRQ_001876"
     CDS             complement(1955..2167)
                     /locus_tag="ACHBRQ_001876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593487.1"
                     /translation="MKIKEQTRKLAAGSSKHCFKVVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(2217..2849)
                     /locus_tag="ACHBRQ_001877"
     CDS             complement(2217..2849)
                     /locus_tag="ACHBRQ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9593488.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2871..3743)
                     /gene="sdaAA"
                     /locus_tag="ACHBRQ_001878"
     CDS             complement(2871..3743)
                     /gene="sdaAA"
                     /locus_tag="ACHBRQ_001878"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9593489.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3752..4423)
                     /gene="sdaAB"
                     /locus_tag="ACHBRQ_001879"
     CDS             complement(3752..4423)
                     /gene="sdaAB"
                     /locus_tag="ACHBRQ_001879"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9593490.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4665..5252
                     /locus_tag="ACHBRQ_001880"
     CDS             4665..5252
                     /locus_tag="ACHBRQ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9593491.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(5305..5625)
                     /locus_tag="ACHBRQ_001881"
     CDS             complement(5305..5625)
                     /locus_tag="ACHBRQ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9593492.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
BASE COUNT         1761 a         1170 c         1318 g         1839 t
ORIGIN      
        1 aaggaggagt agatttctat gaataaaaaa ctaacagatt atgtgattga tctggtggaa
       61 attttaaata aacaacaaaa gcaggttttc tggggaatat ttgatatttt cagtatggtg
      121 gtttccatca ttgtatctta tattttattt tatgggctga ttaatccagc acctgttgac
      181 tacattatct atacgagttt ggccttcctg ttctatcaat tgatgattgg tttttggggg
      241 ttgaacgcga gcattagtcg ttacagcaag attacggatt tcatgaaaat cttttttggt
      301 gtgactgcta gcagtgtctt gtcatatagt atctgttatg ccttcttgcc actcttctcc
      361 atccgtttca tcattctctt tatcttgttg agtaccttct tgattttatt gccacggatt
      421 acttggcagt taatctactc cagacgcaaa aaaggtagtg gtgatggaga acaccgtcgg
      481 accttcttga ttggtgccgg tgatggtggg gctcttttta tggatagtta ccaacatcca
      541 accagtgaat tagaactggt cggtattttg gataaggatt ctaagaaaaa gggtcaaaaa
      601 cttggtggta ttcctgtttt gggctcttat gacaatctgc ctgaattagc caaacgccat
      661 caaatcgagc gtgtcatcgt tgcgattccg tcgctggatc cgtcagaata tgagcgtatc
      721 ttgcagatgt gtaataagct gggtgtcaaa tgttacaaga tgcctaaggt tgaaactgtt
      781 gttcagggcc ttcaccaagc aggtactggc ttccaaaaaa ttgatattac ggaccttttg
      841 ggtcgtcagg aaatccgtct tgacgaatcg cgtctgggtg cagaactgac aggtaagacc
      901 atcttagtca caggagctgg aggttcaatc ggttctgaaa tctgtcgtca agttagtcgc
      961 ttcaatcctg aacgcattgt cttgctcggt catggggaaa actcaatcta ccttgtttat
     1021 catgaattga ttcgtaagtt ccaagggatt gattatgtac ctgtgattgc ggacattcaa
     1081 gactatgatc gtttgttgca agtctttgag cagtacaaac ctgctattgt ttatcatgcg
     1141 gcagcccaca agcatgttcc tatgatggag cgcaatccaa aagaagcctt caaaaacaat
     1201 atccgtggaa cttacaatgt tgctaaggct gttgatgaag ctaaagtgtc taagatggtt
     1261 atgatttcga cagataaggc agtcaatcca ccaaatgtta tgggagcaac caagcgcgtg
     1321 gcggagttga ttgtcactgg ctttaaccaa cgtagccaat caacctactg tgcagttcgt
     1381 tttgggaatg ttcttggtag ccgtggtagt gtcattccag tctttgaacg tcagattgct
     1441 gaaggtgggc ctgtaacggt gacagacttc cgcatgaccc gttactttat gaccattcca
     1501 gaagctagcc gtctggttat ccatgctggt gcttatgcca aagatgggga agtctttatc
     1561 cttgatatgg gcaaaccagt caagatttat gacttggcca agaagatggt gcttctaagt
     1621 ggccacactg aaagtgaaat tccaatcgtt gaagttggaa tccgcccagg tgaaaaactc
     1681 tacgaagaac tcttggtatc aaccgaactc gttgataatc aagttatgga taagattttc
     1741 gttggtaagg ttaatgtcat gcctttagaa tccatcaatc aaaagattgg agagttccgc
     1801 actctcagtg gagatgagtt gaagcaagct attatcgcct ttgctaatca aacaacccac
     1861 attgaataaa aaagaaaaac gcatagtatc aagttacaca accttggtaa tatgcgtttt
     1921 attatgtaga gacttatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc
     1981 gtatatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt tttgagctga
     2041 cttcgtcagt tccatccaca accttaaaac agtgttttga gctgacttcg tcagttccat
     2101 ctacaacctt aaaacagtgt tttgagctgc ccgcagctag tttcctagtt tgctctttga
     2161 ttttcattga gtattacttc attttcttct gaaatggaat tgttacccag tctatgctat
     2221 tgaaaatacg ccaaaacttc taagggtttg tgagcgatat aatcaggttg atagtttagt
     2281 agatctgctt gctctccaaa tccccaagtg atggccaatt tctgaatacc tgtttctcga
     2341 gctcccagca tatcaaactt ggtatctccg atgatgatgg cttgttctgg tgctagttga
     2401 tgtgtctgca aggcttggtg aatgacatct gccttatggg gtgcttcagg gctagaacca
     2461 taaatgccat caaagaaatg atggatttcc aagttttttg ccatgtcttg agcagtagat
     2521 gtatcctttg tcgtggtgat gtagagtgga taactgctcg ataactcctc aagcaagtct
     2581 ataatctgag gaaagagttg agcttcatag atgccttttg ccttatagta agaacgatat
     2641 atctgcacgg cttcagaaat ttggtctttg gacaggcagg tcgcaaaact actttcgaga
     2701 ggtggtccca taaaaccacg aatagttttg gcatcagggc taggcacccc cagctcttta
     2761 aaggtatagg taaaggcatt gtgaatcccg atagaactat caacgagggt tccatccaaa
     2821 tcgaaaaaaa tcgctgtgat agaggtcatg gtttctccta tttgataagc ttattctccg
     2881 aaaatttctt tttggaggcg acgaccagta ggggtggtag cgagtccacc ttcagctgtt
     2941 tcacgaaagg cagttggcat gcttgctcct acttggtaca tggcatcgat cacttcatcc
     3001 acagggattt tagattcgat acctgccaag gccatgtctg ctgcgatgaa agcaaagcta
     3061 gctcccatgg cattacgttt gacacaggga acttcgacca aacctgcaac agggtcacag
     3121 atgaggccta gcatattttt aatgacaaag gcaatagctt gactggcctg ataaggtgtt
     3181 ccacctgcag ccagagtcaa ggcggcagca ctcatagcag aggctgaacc aacttcagct
     3241 tgacacccac cctcagcacc tgagatggag gcattgtttg cgatgactag tccaaaggca
     3301 ccagcagcaa agaggaaatc caattgttgc tcgtggctga ggtctaattt ttcaatagca
     3361 gcagtgagaa cggatggcag acagccagca cttccagcgg ttggagtggc acagaccaag
     3421 cccattttgg cattgtgttc attgactgcg atggcatttc gggcagccga gagaatcgta
     3481 taatctgaca gagtttttcc gttttcgatg tagtgatcca atttggcagc atctccacct
     3541 gtcaggccac tacgagattt attttcattg aggccaagtt ggacagaggc tttcataact
     3601 tccagattgc gttccatgag aaggaagact tcttcacgtt cgcgaccggt caattcaaac
     3661 tctgttgtaa tcatgagttc tgcgacattt ccttgaaagt ccagatctgc ttgctcgacc
     3721 aattctttga tagaataaaa catgcttcct cctatttaaa gaaattgaca ttgtggagat
     3781 gagggatttt tcgaatttct tcgatagcct catcacagtt gcgactgtca acttcgataa
     3841 tcataatggc tttttcacca gctttttcac gagtgacatt catctgggcg atattgatac
     3901 catagcggga aagcgcctct gtaacaaggg caatcatacc tggaatatct tgatgaacga
     3961 tgatgatagt cggtgtattc atattgagag agacggcaaa accattgagt tcggttacct
     4021 gaatatttcc tccaccgata gaaataccag tcacgctgat ggtcttgtgg gcatttttaa
     4081 cagtaatttt agtggtgtta gggtgagggg cattgctgtc tttctgaatg gtccagacaa
     4141 tcttgatacc acgcttgtgg gcaatttcca gactatttgg aatttcagga tcatctgtat
     4201 ccattcctaa aatacctgca acaagggcta ggtctgttcc gtgaccacga taggtcttgg
     4261 caaatgagtt aaaaagttgg aattcaactt ctgtcggagt atcatcaaaa atggaagaga
     4321 caatcttccc aatacgaaca gcaccagcgg tatggctact agatgggcca atcataactg
     4381 gtccgatgat atcaaagaca gattgaaaac gaagtgattt catcagtttc cccttataaa
     4441 aattcttatc tctattatat caaagaatga ggggcttggc tttaattgtg gatgaaaacc
     4501 tttctaatac ctcaaatagc ataaaaatag tatcttttat gacaaaaaac accttattta
     4561 gggaaataaa aaataatttt gtaatatttc tacataaaag tgtcaagaaa cggtaatatt
     4621 taaagggtat gatagaacta tagaaagaag gagaattttc gaatatgaaa tcaataacta
     4681 aaaagattaa agcaactctt gcaggagtag ctgccttgtt tgcagtattt gctccatcat
     4741 ttgtatctgc tcaagaatca tcaacttaca ctgttaaaga aggtgataca ctttcagaaa
     4801 tcgctgaaac tcacaacaca acagttgaaa aattggcaga aaacaaccac attgataaca
     4861 ttcatttgat ttatgttgat caagagttgg ttatcgatgg ccctgtagcg cctgttgcaa
     4921 caccagcgcc agctacttat gcggcaccag ccgctcaaga tgaaactgtt tcagctccag
     4981 tagcagaaac tccagtagta agtgaaacag ttgtttcaac tgtaagcgga tctgaagcag
     5041 aagccaaaga atggatcgct caaaaagaat caggtggtag ctatacagct acaaatggac
     5101 gttatatcgg acgttaccaa ttaacagatt catacctgaa cggtgactac tcagctgaaa
     5161 accaagaacg tgtagcagat gcctacgttg caggacgtta cggttcatgg actgctgcta
     5221 aaaacttctg gcttaacaat ggctggtatt aagaactaac gaacaaataa tatgtaaaga
     5281 gtcgaggaaa tccctcgact ttttttataa tttctttggt tgataatggg tctctacttc
     5341 ttttttgaga tgagacagca tagaagtttc ctcggtcagc tctaggagtg agaacccttt
     5401 ttggataagt ttcgcatcat ccctacaaat cccaatacgg acatagcaga tagccagcct
     5461 atcgtagctt ttcttattct tggcgtcctc cagtaaagtg tagcagattt gcttacacat
     5521 gtttttatct tgattgtata gatagagtgt ggaaaggttt aatagaattg tcattcgcaa
     5581 gtcatataaa tgttgatagt ttttatagac ttctaagcgt tgcaagattt tctcggatta
     5641 ttgacaaata aacggacatt ctgatagacg attgccctag cggcttagtg taaactttac
     5701 aaatttggct tgtaattttt ttataaaatt cacttcagtt acttgacagt tattatattt
     5761 tcgcttatgc ttattatata gataaaagga cgggatagga gtttaaaaat ttgaaagcgc
     5821 tatccataat cataacgcat aattttctcc ttttcgtgaa aaaagttaac gtaattttga
     5881 aatgagctta ataagaggta ctatgtaggt agaaaataaa gataggagaa aaagtatgaa
     5941 taaaaaaata ttttgtattc tagtatgtat tatatcattg attagtctag ctataatctt
     6001 tccatggggc tggtcgatat aatgtaaggg agcttgagtt gttagtggag gataaaatgt
     6061 taagagtaat agtagttatt caatcttt
//
LOCUS       DAYYQN010000084         5901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYYQN010000084 DAYYQN010000000
VERSION     DAYYQN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.085"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            122..436
                     /locus_tag="ACHBRQ_001882"
     CDS             122..436
                     /locus_tag="ACHBRQ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9593493.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            452..838
                     /locus_tag="ACHBRQ_001883"
     CDS             452..838
                     /locus_tag="ACHBRQ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9593494.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            867..2252
                     /locus_tag="ACHBRQ_001884"
     CDS             867..2252
                     /locus_tag="ACHBRQ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9593495.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2255..2407
                     /locus_tag="ACHBRQ_001885"
     CDS             2255..2407
                     /locus_tag="ACHBRQ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9593496.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2430..3632
                     /gene="mobT"
                     /locus_tag="ACHBRQ_001886"
                     /pseudo
     CDS             2430..3632
                     /gene="mobT"
                     /locus_tag="ACHBRQ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
     gene            3681..3845
                     /locus_tag="ACHBRQ_001887"
                     /pseudo
     CDS             3681..3845
                     /locus_tag="ACHBRQ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
     gene            4028..4822
                     /locus_tag="ACHBRQ_001888"
                     /note="aph(3')-IIIa"
     CDS             4028..4822
                     /locus_tag="ACHBRQ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9593497.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            5306..5799
                     /locus_tag="ACHBRQ_001889"
                     /pseudo
     CDS             5306..5799
                     /locus_tag="ACHBRQ_001889"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1952 a         1028 c         1323 g         1598 t
ORIGIN      
        1 ccgattttga aaggaagtga acttatgaaa acaaaaaatc aagaatcaaa aggtcgttcc
       61 ccactcttta agaccatcaa acattcattc agccaataaa aaagaaagga taggtaaaaa
      121 tatggaactt aaatttgtga ttcccaacat ggaaaaaaca ttcggcaatt tagaatttgc
      181 tggcgaggat aaagtcgttc agcgaagaat caacggacgg ctaactgtct tatcaagaag
      241 ctataatctc tattctgatg ttcaaagagc agatgatatt gtggtggtgc ttcctgctga
      301 agctggcgaa aaacatttcg gctttgagga acgtgtgaag ttagtcaatc cacgtattac
      361 cgcagagggc tacaaaatcg gcactcgtgg ttttacaaat taccttttac atgctgacga
      421 catgataaaa gaataaagaa agagaggaaa aatgatgaga ttagcaaatg gcattgtatt
      481 agataaagac acgacttttg gagaattgaa attctctgct ctacgtcgtg aagtgagaat
      541 ccaaaatgaa gacgggtcgg tttcagatga aatcaaggaa cgtacctatg acttaaaatc
      601 caaaggacaa ggacgcatga ttcaagtaag tattcctgcc agcgtgcctt tgaaagagtt
      661 tgattataac gcacgggtgg aacttatcaa tcccattgcg gacaccgttg ctactgccac
      721 ctatcaagga gcagatgttg actggtatat caaggcagac gatattgtgc tgacaaagga
      781 ttctagttca ttcaaagctc aaccacaagc aaagaaagaa ccgacacaag acaaatagtc
      841 gctaggtaga aaggagactt tttcgcatga aacagcgtgg taaaaggatt cgcccatctg
      901 gtaaagattt agtctttcat tttacgatag cgtcactcct gcctgttttc ctgctggttg
      961 tcggactgtt tcatgtgaag acaatccagc agatcaactg gcaggatttt aacctatcac
     1021 aagcagataa gattgacatt ccctatttaa ttatcagttt cagtgtcgca attcttatct
     1081 gcttgctggt agcgtttgta ttcaaacggg ttcgctatga tacggttaaa caactttacc
     1141 accgtcaaaa actggcaaag atgatacttg aaaacaagtg gtatgaatct gaacaggtca
     1201 aaacagaggg tttctttaaa gatagtgctg gtcgtacaaa ggaaaagata acctacttcc
     1261 ctaaaatgta ttatcgactt aaaaatggct tgatacagat acgggtggaa atcacgctgg
     1321 gaaaatatca agaccaactc ttacacttgg aaaagaaatt agagagtggc ttgtactgtg
     1381 agctgacgga taaagagtta aaggattcct atgtggaata tactttgctc tatgacacca
     1441 tagccagtcg tatttctatt gatgaagtag aagctaaaga tggtaaactt cgcttaatga
     1501 aaaacgtatg gtgggaatat gataagctcc ctcatatgtt gattgctggt ggtacaggtg
     1561 gcggtaaaac ttactttata ctgacactga ttgaagcctt gcttcataca gattcaaaac
     1621 tgtatattct tgacccgaaa aatgctgacc ttgcggactt aggttctgtg atggcaaatg
     1681 tctactatag aaaagaagac ttgctttctt gcattgaaac attctatgaa gaaatgatga
     1741 aacgtagtga ggaaatgaag cagatgaaga actataagac tggcaaaaat tatgcttact
     1801 taggtctccc ggcacacttc ttaatctttg atgaatacgt cgctttcatg gaaatgctgg
     1861 gaacaaaaga aaacaccgca gttatgaata agctgaaaca gattgtcatg ttaggtcgtc
     1921 aagctggctt ctttctaata ctggcttgtc aacgtccaga cgcaaaatat ttaggcgacg
     1981 gaatccgtga tcagtttaat ttcagagtgg ctttaggtcg tatgtctgaa atgggctatg
     2041 gcatgatgtt tggcagtgac gtacaaaagg atttcttctt aaagcgaatc aaaggtcgtg
     2101 gctatgttga tgtaggaaca agtgtcatat cagagtttta tactcccctt gtaccaaaag
     2161 gatatgattt cttggaggaa attaaaaagt tatccaacag cagacagtcc acgcaggcga
     2221 cgtgcgaagc ggaagtcgca ggtgtggact gatcttgctg gctggtgtgg caatagccac
     2281 gccagcactt aaccccccgt atctaacagg ggggtacaaa tcgacaggaa acagtcaaaa
     2341 aaacattaga aaatcctttg gttacaaggg atttacaaaa tttcagcgta tgtcaaatgg
     2401 gctttaaaag ttgacatacg cctttttgat tggagggatt tttactgaat gaacaaactt
     2461 ggttacagca tttaaaagaa aaacgcttgg cttatggact atctcaaaac cgtttagctg
     2521 ttgcgactgg tattacaagg cagtatctaa gcgatattga aacaggaaaa gtcaagccat
     2581 cagaggattt acagcagtcc ctttgggaag ctctggaacg cttcaatccc gacgctcccc
     2641 ttgaaatgct gtttgattat gtaaggattc gctttccgac aacagacgta cagcaggtgg
     2701 tcgaaaacat cttacaactg aaactgtcct attttcttca tgaggactat ggtttctatt
     2761 cttattcaga gcattatgct ttaggcgaca tattcgtcct ttgctcccat gaactggaca
     2821 aaggagttct ggtggaattg aaaggtcgtg ggtgcagaca atttgaaagc tatcttctgg
     2881 cacaacaaag aagctggtat gagttcttta tggacgtttt ggtggctggc ggtgtgatga
     2941 aacgccttga ccttgccatt aacgataaga cagggatttt aaatatccct gtactcactg
     3001 aaaagtgcca acaggaagaa tgtatctccg tcttccgcag ttttaaaagc tatcgcagtg
     3061 gcgaactggt acgcaaagag gaaaaggaat gtatgggaaa caccctctat atcggttcat
     3121 tacaaagtga agtttatttc tgtatctatg aaaaggacta cgagcagtac aagaaaaatg
     3181 atattcccat tgaagacgca gaagtaaaaa accgttttga gattcgattg aaaaatgagc
     3241 gtgcctatta tgcagtccgt gatttactcg tctatgacaa tccagagcat accgccttta
     3301 aaattatcaa tcggtatatc cgttttgtag ataaagacga ttccaaacct cgttctgatt
     3361 ggaaactgaa tgaagaatgg gcttggttta ttgggaacaa tcgtgaacga ttaaaactaa
     3421 ccacaaaacc agagccttac tccttccaaa ggacgctgaa ctggctatct catcaagttg
     3481 ccccgacctt aaaggttgcg attaaacttg atgaaatcaa ccagacgcag gttgtaaaag
     3541 acattctcga ccatgcgaaa ctgacagacc gacacaagca gattttgaag caacagtcag
     3601 taaaagaaca ggacgtgata acaacaaaaa aaggatatct gtcaaccata ccagttgaca
     3661 gatatccaaa aaaagatata atgggagata agacggttcg tgttcgtgct gacttgcacc
     3721 atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt atggaaataa
     3781 gacttagaag caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata
     3841 ggagtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     3901 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     3961 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
     4021 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     4081 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     4141 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     4201 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     4261 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     4321 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     4381 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     4441 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     4501 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     4561 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     4621 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     4681 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     4741 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     4801 tttactggat gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag
     4861 ctttttagac atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt
     4921 atatcttttc taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt
     4981 gaagaggttt gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc
     5041 tagcttaaga acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg
     5101 aacaggatta acagagtaac ctctattctt attcagctgc aatcaaaaaa aaataattcc
     5161 tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca gagatatccg
     5221 gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat attttcttgt
     5281 agagggcttc ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg cattgattac
     5341 agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt ttgattcagc
     5401 tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac aggagttgga
     5461 gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg ataacgttat
     5521 tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat atcctgcatc
     5581 aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt tttatgaaca
     5641 gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt tcgtgtggat
     5701 cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg ctctttggaa
     5761 caaatattaa aacaaatgct ttcatataaa aaactatatg ccctacaatg ccgttgtaaa
     5821 aattggggaa aagggatata ttgattttat atctttctca aaataaatat aaaagccctt
     5881 taaagagggc ttttatatat t
//
LOCUS       DAYYQN010000085         5621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYYQN010000085 DAYYQN010000000
VERSION     DAYYQN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.086"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            384..563
                     /locus_tag="ACHBRQ_001890"
                     /pseudo
     CDS             384..563
                     /locus_tag="ACHBRQ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(778..1833)
                     /locus_tag="ACHBRQ_001891"
     CDS             complement(778..1833)
                     /locus_tag="ACHBRQ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9593498.1"
                     /translation="MTLLLNKIYRQDVESSLETIVNINDLNGKSILITGASGLIGSSV
                     IDQLLLLNEIRNFNIKIFCCGREFESLEKRFGRQTEYLHFVFYDATKAPHFDFAVDYI
                     IHAASPASPELYVNQPVETMASNFLGMYNLLEYAKNAKVSNLVYISSSEVYGISTSNN
                     PLDENYIGTVDHLSVRSSYASSKRATETLCVSFASEYGIKIMIVRPGHIYGPSAKDSD
                     NRVSSFFMTEAIAGRDIVIKSTGSQLRSYCYSLDCASAILSVLLSGGSGQAYNVSNPN
                     SIITIKQMATCIADASGVRVLYDIPITQERKRENPMVNASLDSEKIESLGWSGLFSAE
                     VGFHHTFSILKELSSEK"
     gene            complement(1836..2555)
                     /locus_tag="ACHBRQ_001892"
     CDS             complement(1836..2555)
                     /locus_tag="ACHBRQ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFM9593499.1"
                     /translation="MKVAILTASGIGSRIGQDIPKQFIHVENKPVIIYTLEKFQNHPE
                     IDEICIVILKGWDQMVKAYAEQFGITKLKMITFGGKSGQESIYNGLREVKKAHPNDDV
                     TVLIHDGNRPLVSNDIISNALATYQQFGNAVAAIPTTEVVFVLENPQSTSSTEALNRD
                     LLRRTQTPHVYHLDNILSLHEKALENGITDVAASCQLMQLFGEKSYFSLGTEKNLKIT
                     TVEDLDIFKALLSSTRDKWIK"
     gene            complement(2556..3452)
                     /locus_tag="ACHBRQ_001893"
     CDS             complement(2556..3452)
                     /locus_tag="ACHBRQ_001893"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFM9593500.1"
                     /translation="MAVKLKYDKSDITFLDDDELKKYQSELLKMISDVFTFFDENRIA
                     YSLSGGSILGAIRHKGFIPWDDDVDINIPRESYDKLFSLFELDNSLSRKYYLQSAKSH
                     PELGLHVSQIRKKGTVARRKYDHSAEECGISIDLYIVENVYNNPVKRFFQGYTSMFLT
                     FALASVRETKNHALMKEMFRLEGRKLNYSAGKLMVGWFFGIIPIEKWLNWLDKCNSSC
                     KDSHTKYVSIPTGRKHFRRETYLRENMNIYKKVPFETLMVNVPVWSEEYLEMFYGKDY
                     MVVPPVDKREQHLFLELSYGEE"
     gene            complement(3458..4630)
                     /gene="cps2T"
                     /locus_tag="ACHBRQ_001894"
                     /gene_synonym="wchF"
     CDS             complement(3458..4630)
                     /gene="cps2T"
                     /locus_tag="ACHBRQ_001894"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HFM9593501.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKAIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIVYDIAAINRAIKIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQAIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     DLLVCDSKNIEKYIQEDYKQYQPKTTYIAYGTDTTRSILKSSDEKVRSWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASNSKKDFVLITNVEQNKFYNQLLAKTGFDKDPRV
                     KFVGTVYEQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKMIDELDRQSNQRIADSFTWEKIVTDYEKVFKG"
     gene            complement(4661..>5621)
                     /locus_tag="ACHBRQ_001895"
     CDS             complement(4661..>5621)
                     /locus_tag="ACHBRQ_001895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9593502.1"
                     /translation="LKVTKNMEKVLDKLLESDELSWKLVAVSVLDKSDFQHDKIPVIE
                     KEKIIEFATHEVVDEVFVNLPGESYDVGEIISRFETMGIDVTVNLKAFDKNLGRNKQI
                     HEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLILFAIASLVLVPLIRKDGGPAI
                     FAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTMQGGMFKIDNDPRVTKIGRFI
                     RKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPEQKRRLSFKPGITGLWQVSGR
                     SKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
BASE COUNT         1786 a         1155 c          783 g         1897 t
ORIGIN      
        1 ttttttatta caagtcttct attcataaaa tctccgtcaa tcattacttt tcctatttga
       61 aactctaata tttttcaatg aatctctgta attagcaaat gtgtactcca tccgtgttct
      121 gtttgcgggt tacatccatt taccatatgt acatacccta atcaatccat tccacggagt
      181 tcaatatatc caaataaata cagttttccc ttctctataa aattccatca gtggaatctc
      241 tatctttcta tgtactctca taatattcat aacaaaagat actctcgcat gtttttaaca
      301 tatagtccta acatccccat tgataatata gtgtcgtttt ttcaatagat ttcctgcgaa
      361 ataaaatatg gtataatagt tctatgaatt atgaagcaag taaacaacta actgatgtac
      421 gatttaagcg tcttgttgac gttcagcgta ccatttttga agagatgtta gctgtattaa
      481 aaacagctta tcaacttaaa cacgcaaaag gtggacaaaa acctaaatta agcctagaag
      541 accttcttaa ggccactctt caataaaaaa acttctccaa aatcctcttg gatagcttat
      601 atcaaaaatt gactatcttt ttcttcgtat tctttaataa ttgctcttat ttgatcacta
      661 gaattgtcat cacgaatcta tagtttccat gctgtaatgg tttgaaaaag gatactctcc
      721 aactgttcac gtataaattc ttctccattg tatgttgtca tcatgattaa aatactatca
      781 tttttcactt gataactcct ttaaaatact aaatgtgtga tggaagccta cttcagctga
      841 aaatagacct gaccatccca aggactcaat tttctcacta tctaagcttg catttaccat
      901 aggattttcc cgctttcgct cttgagttat aggtatatca taaagaactc tgactcctga
      961 agcatctgca atgcaagttg ccatctgttt tatagtaata attgaatttg gattggaaac
     1021 attataagct tgtcctgatc cacccgaaag aagaacactt agaattgcac tggcacaatc
     1081 caaactataa caataggatc ttaactgact acctgtactt tttatgacaa tatcacgccc
     1141 agcaatcgct tctgtcataa aaaatgatga tacacgatta tcagagtcct tggcactagg
     1201 accatagata tgtcctggcc ttactatcat tattttgatt ccatactcgg aagcaaaact
     1261 aacacaaagt gtttcagtag ctctttttga actggcataa gaagaccgta cgctaagatg
     1321 atcaacagtt ccaatataat tttcgtccaa aggattatta ctagtgctaa ttccataaac
     1381 ttcactagat gagatataaa ctagatttga aactttagca tttttggcgt attccagtag
     1441 attatacata cccagaaaat tactagccat tgtttctaca ggctgattaa cgtataattc
     1501 aggactagca ggactagcag catgaataat gtagtccact gcaaaatcaa agtgaggtgc
     1561 tttagtcgca tcatagaaca caaagtgtaa atactctgtt tgtctaccaa atcgtttttc
     1621 taatgattca aactctcttc cacaacaaaa aattttaatg ttaaaattcc ttatttcatt
     1681 tagcaataat aactggtcaa tgacacttga accaatcaaa ccagaagctc cagtaattaa
     1741 aattgacttg ccattcaagt cattaatatt gacgattgtt tctagagaag attctacatc
     1801 ttgtctatat attttattta ataataatgt cattactatt taatccactt atctcgagtt
     1861 gaactcaata aggctttaaa aatatcgaga tcttctacag tcgtaatttt caagtttttc
     1921 tctgttccaa gagagaaata actcttttca ccaaatagtt gcatgagttg gcatgatgct
     1981 gcaacatcag ttatcccatt ttctaatgct ttttcatgaa gcgatagaat attatcaaga
     2041 tggtaaacat gaggagtttg tgttcgtctc aacaagtcac gattcaatgc ttctgtggac
     2101 gaagttgatt gtgggttttc taaaacaaat actacctcag tcgttggtat cgctgcaaca
     2161 gcatttccaa actgttggta tgttgctaaa gcgttgctaa taatatcatt gctcactaaa
     2221 ggacgattcc cgtcatgaat taatacagtc acatcatcat tgggatgagc tttcttaact
     2281 tctctaagac cgttataaat tgattcttga ccagacttcc ccccaaaagt tatcatcttt
     2341 aactttgtaa ttccaaattg ttccgcataa gctttaacca tctgatccca gccttttaaa
     2401 ataacaatac agatttcatc aatttctgga tgattttgaa atttttcaag ggtatagata
     2461 atgactggct tattttcaac atgaataaat tgtttaggga tatcttgacc aattcggctt
     2521 ccaatgcctg aagctgttaa aatcgctact ttcatctatt cttctccgta actcaattct
     2581 aaaaacaaat gttgctctct cttatcaaca ggaggaacga ccatataatc tttaccgtaa
     2641 aacatttcta aatactcttc tgaccaaaca ggaacattca ccatcaaagt ttcaaaagga
     2701 acctttttat aaatattcat attctctcgc aagtaagtct cgcgtctaaa gtgtttcctt
     2761 cctgtaggta tggatacata ttttgtatgt gaatccttac agcttgaatt acatttatca
     2821 agccaattca accacttttc aattggaata attccaaaaa accaaccaac cattaacttt
     2881 ccagctgaat aatttagttt tctaccttcc agtctaaaca tttccttcat cagggcatga
     2941 ttctttgttt ctcgtacaga agccaaggca aaagtcaaaa acatacttgt ataaccttga
     3001 aagaatcgtt tcactggatt attgtataca ttttctacaa tgtaaagatc tatagaaata
     3061 ccgcactcct cagccgaatg atcgtatttc ctacgagcaa ctgttccttt ttttctaatt
     3121 tgagaaacat gaagtcccaa ttctggatga ctttttgctg attgaagata atatttacga
     3181 ctaagactat tgtctaactc aaatagagaa aatagtttat cgtaactttc cctcggaata
     3241 ttgatatcaa catcatcatc ccaagggata aaccctttat gacggattgc ccctaaaatg
     3301 ctacctccac ttaaagaata cgctatccta ttttcatcaa aaaaagtaaa cacatctgat
     3361 atcattttca acaattctga ttggtatttt ttcaattcgt catcatctaa aaatgtaata
     3421 tctgatttat catattttaa ttttactgcc attaactcta acctttaaat actttctcgt
     3481 agtctgtgac aatcttttcc caagtgaaag aatccgcaat tctctgattt gattgtctat
     3541 ctaactcatc aatcattttt gcatcaaatt gttcaacttt ctcgattact tgggaaagct
     3601 catctttttt ccaataaatc gcactttgct ccccaacttc tcggttaaag ccaacatcta
     3661 gcaataaatt cagtttagtt gatgccagag cttctaaaag tgaaggattg gttccaccaa
     3721 cttcatgccc gtggaagtaa gcaaaggcgt tttcacgaat atatttaaga agctcttgct
     3781 catagactgt acctacaaat tttactcgtg ggtccttatc aaaaccagtt tttgccaaca
     3841 actgattata aaatttattc tgttctacat ttgtgattaa gacaaagtcc ttcttagagt
     3901 tagatgccaa aaagccacga atcatagatt catagttgtt ttctggtaca aaacgtccta
     3961 caactagata atactcgttc tcagaaacat tcttctcttt gaaccaagaa cgtacttttt
     4021 cgtcactact ctttaatatg gagcgtgttg tatcagtccc ataagcgata taggttgtct
     4081 taggttgata ctgtttataa tcttcttgga tatatttttc aatattctta ctatcacaca
     4141 ctaaaagatc tgcatgtttg accataagac cttcggaaat tttccaataa cgacgaactg
     4201 gagcgctcca cttagctcgt aaccactcgt gaccatctgg attaactaga agagtcccac
     4261 ctatcgcctg gatttttttc ttaattccat ggatgaacgg accgattcga caagctagaa
     4321 tatagaagat tggatcttca tccttatttt ctttggcaat tttaatagct ctgttaattg
     4381 ccgcgatatc atatactatg gctcgcgctg gaccaatatt aggaacatcg acgttataac
     4441 agatagcacc attatgttca aaaacatcct cagtagtccc tgattttgca gagttttcac
     4501 gcatacaagc cacataatat tggatagcct tatcttgttg gaaggctgtt aatttttcaa
     4561 caaaagtttc aaatcctcca tacttagcag gaattccttt tgaaccaatg atataaactg
     4621 actttttcat ttctctccta atatgatgga tatacagaaa ttacttcgct ccatctctca
     4681 taaatacaac tttaacagtt ttaagcaaaa tttcaatatc tttccagatt gtccaattat
     4741 caatataagc cacatctaat tttacaacat catcaaaatc ggttatttta ctacggccgc
     4801 taacctgcca taaacctgta ataccaggtt taaagctgag tcgacgtttc tgttctggag
     4861 tatactgatc atactcgtct actgtaggtg gacgtgttcc caccaaactc atatctccta
     4921 taaagacatt ccaaaactgc ggcaattcat ccaaactggt tttacgaata aagcgaccaa
     4981 tttttgtaac acgaggatca ttgtctatct taaacatacc accttgcatc gtattttgat
     5041 ccatcaactg ttctttgata gcttcagcat cgatccgcat cgaacggaat ttataaaagg
     5101 taaaatgtcg accatttttc cctatacgag tttgagcaaa aatagctggt ccgccatctt
     5161 tacgaatcaa tggaactaaa actagactag ctatagcaaa aagaataagg ccaatagtgg
     5221 caccacaaat atcgagaatt ctctttgaaa tcacatgact agttttataa aaatttgtag
     5281 agaaagtgac tacattcaat cccaccatct catgaatttg tttattgcga cccaaattct
     5341 tatcaaatgc tttaagattt acagttacat ctatccccat tgtctcaaac ctagagataa
     5401 tttctccaac atcgtagctc tctcctggaa gattgacaaa cacctcatcc acaacttcat
     5461 gcgttgcaaa ttcaataatt ttttcctttt caattacagg tattttatca tgttgaaaat
     5521 cagatttatc caaaacactt actgctacca atttccatga aagttcatca gattctagca
     5581 atttatcaag aactttttcc atatttttcg ttacttttaa c
//
LOCUS       DAYYQN010000086         5354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYYQN010000086 DAYYQN010000000
VERSION     DAYYQN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.087"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..2688)
                     /gene="ppc"
                     /locus_tag="ACHBRQ_001896"
     CDS             complement(<1..2688)
                     /gene="ppc"
                     /locus_tag="ACHBRQ_001896"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFM9593503.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRN"
     gene            complement(2708..3937)
                     /locus_tag="ACHBRQ_001897"
     CDS             complement(2708..3937)
                     /locus_tag="ACHBRQ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9593504.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(4065..4389)
                     /locus_tag="ACHBRQ_001898"
                     /pseudo
     CDS             complement(4065..4389)
                     /locus_tag="ACHBRQ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(4835..5056)
                     /locus_tag="ACHBRQ_001899"
     CDS             complement(4835..5056)
                     /locus_tag="ACHBRQ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9593505.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
BASE COUNT         1683 a         1091 c          929 g         1651 t
ORIGIN      
        1 attacgcaat cctgtcgcaa ttccgttgat ggtgatatga atcaatcgtt cttgatcact
       61 ggacaattct ccacgacgtt ggcgtttaat caactctaac tgaatatagt tgagaatatt
      121 aaagtaaggc atacggtaat ccagactagc ttttagatat ggattgtcag ctaagagttc
      181 gtcatgtcct tcaatagcca agataacgtt cttagtaact tgccattcat ttaaaatagt
      241 ctcatagatg gccttaactt gctcgtcttc acaaagttta gcatattcaa aagcaatatt
      301 catatttgat tttgacaaaa ccatatcaac atttgaaaga agcgattgga agaaaggcca
      361 attttggtac atatctcgta agatagcaat attctctgga tttttattga taaattcctt
      421 gaagcttgaa ccaaccccgt accatccagg gaacataaca cgactctgtg accatgagaa
      481 tacccaaggg atggcacgca aaccaccgat ttcagtaata gtcttacgag cggctggacg
      541 agaaccaata ttaaaacttg aaatagcctt gattggactt gactcgaaga aataatcata
      601 gaaatgctca ttaccaaaga ccaaatcacg gtagatatcg taactacggt ccactacttg
      661 atccataatg gcttcataac gatttggggt attggtatcg ctcttcttct gagtaatcat
      721 acggttaata gctgccgata ctagcatttc aaggttatag taagcggcgt ctttgttacc
      781 gtatttattc ccaattactt caccctgctc cgtcaagcgg atacgatcct tgatagactt
      841 gagcggttga gatgtaatgg cttcataggt tggcccacca ccacgaccga cagtaccacc
      901 acgaccatgg aagaaggtaa ccttaacgcc aaattcatct ccaatagcag tcaattgttg
      961 ttgagccttg tagagggtcc aacatgatga caagtaaccg ccatctttat tactgtcaga
     1021 gtagccaagc atgatttctt ggtagttatt tcgtgagtca atccattttt tggcaaggct
     1081 aagagaaaga tattttctca ttgtttcttc tgaatgatcc aagtcttcaa ttgtttcaaa
     1141 aaggggaaca atctgaacac gcgccctttc cgtatccacc agtcctactt cttttaacag
     1201 aatagctaat tctagcatat cagaaaggct ggttgcatgt gaaatgatgg tctgacggat
     1261 gacatcatct cccaacttat ctttcaaaac acgagccgtc ttaaaaatag ctaattcttt
     1321 tgctaataat tctgattttt ctgcgtgagt cgcagaaaga attcggggat cttcttctaa
     1381 ttctttcaag agaaggtcac acttttcttc ttcgctcaac tcgctataac gagaatgaat
     1441 tcctgctgat ttcaagagtt ctgccacaca ggcttcatag acgctagagt cttgtcgcat
     1501 atcaattgat gctaggtaaa aaccaaatat ctctactgcc tgcaataatt ccacaaaatc
     1561 acctgaaatc aaggactcac ccttattttc tagtagagaa tctcgaatgg caatcaaatc
     1621 cttgtagaaa tcattggcgg tttcataacg agtcccaact tcttcatcct caatcagata
     1681 ggttttagtt gcctgaattt ttgattgaat atcaaacaag gcacgacggt aaagctcttt
     1741 ttcgcggtaa atcgagttat ccttggattg acgagccatt tctctgactt gcttgctgac
     1801 gttgacaatg ctagttgaaa gagaaaattc acgataaagt tggtaaatct ttttatcata
     1861 gtagttcatg atgacttcac attgagtgag tgcagactgc ttcaaggtct ctgctgtaac
     1921 aaatggattt ccatcacggt caccacctat ccacataccc atggtgattg gtttagcctg
     1981 ttttaaattc agaccatgcg cttgcgctaa gcgcttatac tccgtcgtca aatgaggtac
     2041 agctttcaaa aaggagctgt tataatattc catagcattc gtgatttcgt tagtcacttt
     2101 taattttttc tcacgaatca tgtctgtctg catgataatt tcgatgtaac gacgcaaatc
     2161 attgtaccat ttatctttat tgatcaaccc caacttaaca tcacggtatt tacgcaaaag
     2221 actatgaata tgatttgtta aatccaacat acttttgcgt tgcacttgtg ttggatgggc
     2281 tgtcaaaaca gggacaacat tcaagtgttc taggatctca acggcatttt ccttttctgc
     2341 taccaattta atcgttgtag ataatttacc taaatagtcc tgatcaatat tattttgatg
     2401 attgatttca taagctaaat ccacatcctc tgaaatattg atcaaaagag gtaagataga
     2461 gaaatagcgt gaaatataga ccatttcatc atttgataag ctagtcacta gacggtttag
     2521 accttgataa tcttcctgcg ttgataattc tttcaactgt attatttttt caaaggtctc
     2581 tggggcaagc atatttttag taatatcttc cagtaattct gttagaatca agacttcttc
     2641 ttgcacaaca cttttattac tataattttc taatttttga agagacataa actatccttt
     2701 ctttatccta cttcaacaga aggttcattg gttgattttc caattctcgg tacaacttag
     2761 cgcgtttttc actggcatca atatttaaga caaaggctac tgccactgat aagactagaa
     2821 gactatttcc accctgggat aagaagggga aggttactcc tgtagatgga atcaagcccg
     2881 aaatccctcc gatattgaca aatacctgaa ccaacatcat ccctccgaca ccgagtgcaa
     2941 ccatggcatt gaaaggattc tccgctcgaa taccgaccaa gataatccgc aaaatcatga
     3001 aaaacaagag agctaaaata agactggcac cagcaaagcc aaattcttca atcacgatag
     3061 aaaagacaaa gtctgtatga gcttctggca aataacctcg tttttcaatc gagtttccaa
     3121 gacctagacc aaaccaacca ccattgacca tggcaaaata agaattagct aactggtgac
     3181 ctgcatcagc acgatcggca aaaggattaa aaaaggcact aaagcgcttg gctacatagc
     3241 caaatactgg aatttttgaa aaggtctcaa caccgattag gctgatagtg gtcaagacaa
     3301 agacagaagc ggcagatacg agcgccagaa tggttgaaaa ccagcgataa gcgattccac
     3361 taactgtata cataatcaag gaaaccaaga ctaaaatagt cgcatttcct aaatcaggga
     3421 aaattcccaa acttccaatc agaactagga gaacgaatcg ccaatcatta aaagcacggg
     3481 gaagccattg attttgagtc aaaacttgaa aatcataagt agctatttct tcttgctgtt
     3541 tggagaatcg gtgagctaaa taccaaataa taatgatttt taagtactca gctggctgaa
     3601 tagttactcc tgcaaccgaa atccaaccgt atgccccgtt tacggaaata ccaataaaac
     3661 gagccaagaa caataaaagc atttctatta atataactaa aatgattagt cgctcatttc
     3721 tcaaaaaatc tagtctcaat ttataaatta aggcaatcag tatcaaacta acaatccaaa
     3781 agattccttg gtttcgaacc aactgcaagg cgctcttgcc ttcttcaatt aaaatagcac
     3841 tggtggtcga atagaccaca atcaagccca aaatagataa aagcaagtag ggaatcaaga
     3901 tggaataatt taataagtgc ctcttactaa tcttcatagc atcaccactc tactaggata
     3961 cagacaaatc tgttccaaat tccgttttat tttctagttt tgattgctat tataccattt
     4021 tttcagaaag aaaaaaactc ttaccttaca atccttcgaa ttttactgtt tttcataatt
     4081 agggcagaat ttgaaatcgt aaaaccattt tgaacaagag ttacttcaac ccattggctc
     4141 cgacggatta agttgctttc gtgaatgcca aaatcagccg caatttgttc ataagtgcgc
     4201 tattctcgca tgtattgaat agttaccatg aggagatcgt ctaggcttaa tttggttttt
     4261 cgtccacctt ttgcgcgttt acgttgcgtt gataagctgt ttttaacaca gctaacatct
     4321 cgtgcgctga acacctacaa ggctcttgaa acggctatcg cttaattgtt tacttgcttc
     4381 atcatccata gttttattgt attgtatttt tgtttcgcag gaagtctatt attcctttcg
     4441 ttccaaggtt tttacgcttt tagcaacaag cttacacact ccacgtgatg cgtctgtgga
     4501 actcaaaagg ttcggggaac cttttgagga ttaattgcgt ttctctaaca aactcacaca
     4561 tttaacgtgg catgactgat gaagtcatca taagaatttc acttgctgct cttttacgat
     4621 ggcagcctga acggtcagaa gtatcattgt atatcatttg tatcatggca tataaagtat
     4681 cgctctattt tattgatggt tttaaaatcg ctcttatcat ggcgagccat tcaaatttgc
     4741 tccacaaatg aggaaagtac cattttggac tatttaattt tcaaagaaca aataaaaaat
     4801 ctcctcattt tcacactcta tgaaattgag gagattattt tctaaagcga attttatcag
     4861 gaaaatctgt caattccaat aggtagtctg tactgacatc gtaaaagttt gagagagtta
     4921 ccaatacatc tgctgttaac gtatgctcac cccgttcaat tttagcataa gctgagtttg
     4981 taaacgaaag tattttagct atttgttttt gggtcaaatc gtgatcttcc ctcaaatctc
     5041 tcaaacgtct gtacatctag ctttctcctg agaaaagtat aatatataaa actaagaaat
     5101 cattcaaagt gtcctaactg gacattttga actaaaatta atttattaaa catatctcat
     5161 aagcatatct attataggta agacaatatt aagaaagacg gttaaaaaac aacctgactc
     5221 cactggaatt caggaatcag gtcgcttaag attttatatt attttactgt ctacttgaca
     5281 gggagctgtt caccaatggg aatctatagc ttttttgtga ctgagaacta ttttatctca
     5341 gacttcttca acac
//
LOCUS       DAYYQN010000087         5009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYYQN010000087 DAYYQN010000000
VERSION     DAYYQN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.088"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            133..351
                     /gene="nrdH"
                     /locus_tag="ACHBRQ_001900"
     CDS             133..351
                     /gene="nrdH"
                     /locus_tag="ACHBRQ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9593506.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            432..2591
                     /gene="nrdE"
                     /locus_tag="ACHBRQ_001901"
     CDS             432..2591
                     /gene="nrdE"
                     /locus_tag="ACHBRQ_001901"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9593507.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDEEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2779..3741
                     /gene="nrdF"
                     /locus_tag="ACHBRQ_001902"
     CDS             2779..3741
                     /gene="nrdF"
                     /locus_tag="ACHBRQ_001902"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9593508.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            4029..4790
                     /locus_tag="ACHBRQ_001903"
     CDS             4029..4790
                     /locus_tag="ACHBRQ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9593509.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         1608 a         1019 c          904 g         1478 t
ORIGIN      
        1 acacaaacac tatatattgt gtttatatat aaaaacaaca aaagaacacc actatattta
       61 gtttaaatca ttgacaaaat tttattttct gatatactaa gatagtatta aattttgaag
      121 aggagttaca caatggtaac cgtttattct aaaaacaatt gtgtccaatg taaaatgacc
      181 aagcgtttct tggacagtaa taatgtctct tatcgtgaaa tcaatcttga tgagcaacct
      241 gagtacgtcg atcaagttaa agagctcggt tttagcgcag ctcctgttat ccaaacacca
      301 actgaagtct tttcaggttt ccaaccagga aaactgaaac aattagcata atcttagtac
      361 atcatccaga agaaactgct tttagggcta acttagaagc ctttcttttg taattagata
      421 agggaaattt tatgggatta aaacatcttg aagacgtgac ttacttccgt cttaataacg
      481 aaattaaccg tcctgttaat ggacaaatca tgcttcataa agatgaggaa gccttggatg
      541 ctttctttaa agaaaatgta gttccaaaca ctatggtttt tgattcaatc aaagacaaaa
      601 tcaattacct cattgaacac aactacatcg aaacagcctt tatcaagaaa taccgtccag
      661 agttcttgga agaattggct caatttatca aagaccaaaa cttccaattc aagtcattca
      721 tggcagctta taaattttac aatcaatatg ccttaaagac taatgacggt gaatactatc
      781 ttgaaaatat ggaagaccgt gtcttcttca acgcccttta tttcgctgat gggaatgaag
      841 ctgttgcaat cgatattgcc aatgaaatca tccaccaacg ctaccaacct gctactcctt
      901 ccttcttgaa tgctggacgt gctcgtcgtg gggagttggt atcttgtttc ctaatccaag
      961 tgacggatga tatgaactct atcggacgtt ctatcaactc agctcttcaa ctttcacgta
     1021 ttggtggtgg tgtgggaatt accctcagca accttcgtga agctggtgca cctatcaaag
     1081 gctatgaagg agctgcttca ggtgtcgttc ctgttatgaa gctttttgaa gacagcttct
     1141 cttactccaa ccaattgggg caacgtcaag gtgccggcgt tgtctacctc aacgtctttc
     1201 acccagatat catcgctttc ctttcaacta agaaagaaaa cgctgatgaa aaagtacgtg
     1261 tcaagactct atcacttggt gttgtagtac ctgataaatt ctacgaattg gctcgtaaaa
     1321 atgaagaaat gtacctcttc agcccttact ctgtagaaaa agaatacggt gtgccattca
     1381 attacatcga cattactgaa aaatacgatg aattggtcgc aaatccaaat attcgcaaga
     1441 caaaaatcaa ggcgcgggat ttggaaactg aaatctctaa attgcaacaa gagtctggct
     1501 atccttatgt agtcaacatc gatacggcta accgtgcaaa tcctgtcgat ggaaagatta
     1561 tcatgagtaa cttgtgttct gagattcttc aagttcaaga accaagcttg atcaacgatg
     1621 ctcaagaatt ccttcaaatg ggaacagatg tttcatgtaa cctgggttca accaacgtgg
     1681 ttaacatgat gacttcacct gattttggtc gttctatccg tgctatggtt cgtgccctta
     1741 ctttcgttac agatagttca catatcgtag ctgtaccaac gattgaccat ggaaatagcc
     1801 aagcccacac ctttggtctc ggtgccatgg gactgcacag ctaccttgcc caacaactca
     1861 ttgaatatgg ttcacctgag tctgttgaat ttacaagcat ctactttatg cttatgaact
     1921 actggacctt ggtagagtca aacaatattg cgcgtgaacg tggtattacc ttccacaact
     1981 ttgaaaaatc agactatgct aacggaagtt actttgacaa gtatgtaact ggtgaatttg
     2041 ttccaacatc agaccgtgtt aaagaactct tcaaaaatgt ttttatccct ggtgttgctg
     2101 attggactga acttcgcgac aaggttcaag aggatggtct ttaccaccaa aatcgccttg
     2161 ctgtagcgcc aaatggttct atcagctaca tcaacgatgt ttctgcttct atccacccga
     2221 ttacgcaacg tattgaagaa cgtcaggaaa agaaaattgg taaaatctac taccctgctg
     2281 ctggcttgtc tacagaaacc attccttact acacttctgc ctacgatatg gatatgcgta
     2341 aagtcatcga tgtttacgct gctgcgactg aacacgtgga tcaaggactt tcactcaccc
     2401 tcttcatgcg tagtgacatt ccaaaaggcc tttacgaatg gaagagggaa aataaacaaa
     2461 cgacacgtga cttgtccatc cttcgtaact atgcctttaa caagggaatc aagtctatct
     2521 actacgtccg tacctttaca gatgacggtg gagaagtcgg cgccaaccaa tgtgaaagct
     2581 gtgtgattta gtttctagca tagaaacact atttttccct aagcagcact gataaaatca
     2641 aatctactat gtcataacat aatttatgta acattttaaa agtcgggctt ccgacttttt
     2701 gtgcgatact cctatagaat tatggtaaag tagaaataat tgtgagttcg caatactaaa
     2761 cacagaaaga gattttaaat ggaaacttac tacaaagcca ttaactggaa tgccatcgaa
     2821 gatgtcatcg acaaatcaac ttgggaaaaa ctgacggagc aattttggct cgatacacgt
     2881 attcccttat caaacgactt ggatgactgg agaaagctat ctaacaaaga gaaagacttg
     2941 gtcggaaaag tttttggtgg tttaaccctt cttgacacta tgcaatctga aactggggtt
     3001 caagcccttc gcgcagacat ccgtacacca catgaggaag ctgttttcaa taacatccaa
     3061 tttatggaat ctgtccacgc taaatcttac tcatcaatct tttctacctt gaatactaag
     3121 gctgagattg aagaaatttt cgaatggacc aataccaatc cttacctaca aaagaaggct
     3181 gagattgtca acgaaatcta cctaaacggc agcccacttg aaaagaaagt tgccagcgtc
     3241 ttcctcgaaa ccttcctctt ctactctggt ttcttcactc ccctctacta tctcggtaac
     3301 aacaaacttg ccaacgttgc agaaatcatt aaattgatta ttcgtgacga gtctgttcac
     3361 ggaacctaca ttggttacaa attccaactt ggtttcaatg aattacctga agaagagcaa
     3421 gaaaaactca aagaatggat gtacgaccta ctctatactc tttatgaaaa tgaagaaggc
     3481 tacacagaga gtctttatga cggtgttggt tggactgaag aggttaaaac cttccttcgc
     3541 tacaatgcca ataaagctct catgaacatg ggacaagatc cacttttccc agattcagct
     3601 gaagatgtca acccaatcgt tatgaacggt atttcaacag gaacttccaa ccatgacttc
     3661 ttctctcaag tcggaaatgg ttaccttctt ggcgaagttg aagccatgca agatgatgac
     3721 tacaactacg gtttggacta atactcttcg aaaatcaaat tcaaaccacg tcagcttcgc
     3781 cttgccgtac tcaagtacag cctgcggcta gcttcctagt ttgctctttg attttcattg
     3841 agtataattc tccaccactc ttaaccatca aaaagtaaac tagaaacaac aaaataataa
     3901 cccaacaaaa atatcgaaag ttttgaaaac aaacttcgat atttttgttt ttgtttcatt
     3961 ttgttgtttt tgtatttgaa tgatgagttt gtttatggta agatagtagt aggaaacgag
     4021 gtgataatat gctcaaacaa gaaaaattaa ccaaaatttt agagatagta aatagtaaag
     4081 gaaccataac agttaaacag attatggacg aaatagccgt ttcagatatg actgcaaggc
     4141 gctatttaca ggaattagct gataaagatt tgctgattcg tgtgcatggt ggagctgaaa
     4201 aacttcgaac caactccctt ttgactaatg agcgatcaaa tattgaaaaa caagccctcc
     4261 aaacggcaga aaaacaagaa atagcccatt ttgcaggcag tctagtagaa gaaagagaaa
     4321 ctattttcat tggaccagga acaacattag agttttttgc gcgtgagttg cctattgaca
     4381 atatccgcgt cgtaaccaac agtctacctg tttttctgat tttaagcgaa cgaaaattaa
     4441 cagatttgat tttgataggt ggaaattatc gcgatattac aggtgctttt gttggcacat
     4501 tgaccctaca aaatctctct aatctccaat tttctaaagc tttcgttagc tgtaatggta
     4561 ttcaaaacgg agctctagct acttttagcg aggaagaggg agaggctcaa cgcatcgctt
     4621 taaataattc taataaaaaa tatttactcg cagatcatag taagttcaat aagtttgatt
     4681 tttatacttt ttataatata tcaaatcttg atactattgt ttcagattct aaactaagtg
     4741 attcaatcct ttttaagcta tctaaacaca ttaaagtcat caagccttaa taaactgaaa
     4801 gagcctgggt aacaactcaa tttcagaaga aaaaaagtaa atcttcccac aataaaaagc
     4861 atagtatcag gtgtttcaac acctgatact atgctttttt ctatatcaaa gactttttgc
     4921 ccaattcctc aaagctatgc attgagaaat ctgcgactcg cttcctagtt taaagttgct
     4981 ctttgatttt cattgagtat aagcaacgt
//
LOCUS       DAYYQN010000088         4812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYYQN010000088 DAYYQN010000000
VERSION     DAYYQN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.089"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(252..803)
                     /locus_tag="ACHBRQ_001904"
     CDS             complement(252..803)
                     /locus_tag="ACHBRQ_001904"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9593510.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1201..2025)
                     /gene="nadE"
                     /locus_tag="ACHBRQ_001905"
     CDS             complement(1201..2025)
                     /gene="nadE"
                     /locus_tag="ACHBRQ_001905"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9593511.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRSETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2022..3482)
                     /locus_tag="ACHBRQ_001906"
     CDS             complement(2022..3482)
                     /locus_tag="ACHBRQ_001906"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9593512.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEGLRFSDSDIAYLELLGYHGAFLNYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3598..4086)
                     /locus_tag="ACHBRQ_001907"
     CDS             complement(3598..4086)
                     /locus_tag="ACHBRQ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HFM9593513.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4076..4285)
                     /locus_tag="ACHBRQ_001908"
     CDS             complement(4076..4285)
                     /locus_tag="ACHBRQ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9593514.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1404 a         1081 c          886 g         1441 t
ORIGIN      
        1 taacaacagc taggattaca ctataaaaag tacattacca cctgttcggc tggagaatct
       61 agtcgattag ctccctctta gttacaagct taaagcgatt ttttaactaa cactaaacca
      121 gattaaagaa ggaactagaa catctgtctt gtactaatca atatgctatt ttaaagtttc
      181 aaataaactt ggaatgctaa ttattatatt tacaataaat agttgacttc gatagaaaca
      241 gaaaatagaa ttcaacattc ttccctactt ataccataat aaataagatc cgcaagatag
      301 ccttttctct ctacaccatt aacaatggtt tgtagatagg acattccagc cttttccatg
      361 acacgacctg aagctgggtt gagactggca taacgtgctc tgaccttttg aaaacctgct
      421 tgagtaaaac aaaagtccaa gatagctttc aaagtctctg tcatcatacc atttccccag
      481 taagccttgc ctaacacata gccaatttca cagcttaaat cagcctcgtc tatcttaaca
      541 atgctgatat ctcctatcac ttgctctggg ttttctttta gacaaatggc ccatttataa
      601 tagttgagat tagtatagga agcaacccaa ttgcaaatcg agtttcgagt gatttcgaca
      661 tcaggatggg gatcccaggt aacataggtc agattctcag cggatgaagc ccaattttga
      721 aacatggctt ctgcatcact ctccacaaat cttcttaaaa tcaaacgttc tgtctgtaat
      781 atttgcgtac cgatagattt catgatgcta cctcacttcc ttatttctat actcgtagta
      841 aaaaggctca actcagtaac agctagaaaa cgagtttgtc ttctaatttc ttgacgcagt
      901 tgctgcctgg tttgaaatgc gctctagcaa gctttttcaa acctagtcat cgttgcaggg
      961 gtaagacgat gaaatcgaac tcttcgagtt actgaatctg cctcattttc ttatttctaa
     1021 actcggggtt caagatcccc cagaccttct ctcactttct tatttcaaga tgagaggctg
     1081 aaaacctcca gtggaggttt ttcttgcttt ttaatttcag ggcaagaagc taaaaagtcc
     1141 cccggacctt cttcgctttc tcatttctag gctcagggta aaaacctcca atggaggttt
     1201 ttactcccaa aagtcatcaa atacagtgat gggtaagtgg cgtttgtgtt ggcctttgtg
     1261 ccaccagttt tcaatggtcg cttgagcttc tgggctgatt gttttgcctt ctaggtagtc
     1321 gtcaatctct gcgtaggtga ctccaagtgc gacttcgtca gctaggcctg gtttatcttc
     1381 ttctaggtct gccgttggga ttttttcata aagggctggc tctgcgccaa gtttctgcaa
     1441 gagctgtttt ccttggcgtt tattgaggcg gtaaagaggg agaatatccg caccgccgtc
     1501 accaaacttg gtaaagaaac ctgtgatatt ttccgcggcg tggtctgttc caatgaccgc
     1561 tccgctatgg gaaccagcaa gggcatactg agcaatcata cggcaacgtg ccttgatatt
     1621 ccccttgttg aagtctgaaa caggactacc tgtcgcttca actgcagctg tcatggcatc
     1681 agctgattcc ttgatattca caaccaagct gacatctggc tggatgaagg ctagagcttt
     1741 ttgagcatct gcttcatcag cttgcactcc gtatggcagg cggacagcga taaatttgta
     1801 gctgtcgtct cccgtttcag atcgcagttc ttccatagct aattgcgcca aacgccctgc
     1861 caaggttgag tcttgtcccc cagaaatccc tagtacaaag gtttttagga agggatgttt
     1921 tttcagatat ctttttaaga aatcaataga acgacggatt tcttcctggg catcaatcac
     1981 tggtttgaca cccagctctt ggataatcgt ttcttgcaaa ctcattcttc ttctccttca
     2041 ccaagggctt ccttgcgcat cttatcaatc aagtccatct tatcttgcca tacatcacga
     2101 gccaaatcca ctggatagtg ctgcggattg agcacacgct tatactcatc ccacaacttg
     2161 tcaaattcct tacgggcata atcctgaatg tcagtcaaac taggcaagtt gtaaactaat
     2221 attccttctt tgaagatatc caccaagaga ggaacggcat caaaattacg aaccgtcttc
     2281 ttgatgtatg tataggtcgg atggaacatc ttgatttctg tcatgtcgct aatatccaca
     2341 ccatcataag tgatatagtc gccttctgac ttgccttttt cacgactggt aatgcgccac
     2401 acctgcttct tacctggcgt agagactttt tccgcattat tagacagctt aatcgtattg
     2461 cgcatctgac cagtttcatc ttcgattgca acaatcttgt aaactgcacc aagagctggc
     2521 tggtcatagg ctgtaatcag cttggtaccc acgccccaga catcaatctt ggccttttgc
     2581 atcttgaggt tgaggatagt attttcgtcc aaatcattag aagcataaat cttagcctct
     2641 gtaaatccag cctcgtccag ttgttgacgg actttcttag aaatgtaggc aatatcccca
     2701 gagtcaatcc gcacacccat aaagttaatc tgatcaccca gctcacgcgc cacctgaatg
     2761 gcagctggta caccgatgcg aagggtgtca taggtatcca caagaaagac acaatttttg
     2821 tgggtcgcag cgtaagcctt gaaagcttca tagtcattgc cataaacctg taccaaggca
     2881 tgggcatggg ttcccaaaac aggaatgtca aagagcttac ccgcacgcac gttgctggtt
     2941 ccattggcgc caccaatcac cgctgcgcgt gttccccaga tggccgcatc catttcttga
     3001 gcccgacgtg tcccaaactc catcaagggt tcatcttcga taaccgaacg aatacgagct
     3061 gccttcgtcg ccaccaaggt ctggtagttg acgatgttca aaagagccgt ttcgaccaac
     3121 tgacattggg ctagaggtcc ttccacctgc acaatcggtt cattagcaaa aaccaaatcc
     3181 ccttcttggg cagaacgaac ggtcaactcc aacttgaaat tgcgaaggta attcaagaac
     3241 gccccatgat aaccaagcaa ctccaaatag gctatatcac tatctgaaaa acgcaagcct
     3301 tcaagatagt tcacaattct ttccaaacct gcaaaaaccg catagccgtt cttaaaaggc
     3361 tgttggcgga aatacacctc aaagaccgcc ttcttattgt gaatcccttg gtcaaagtaa
     3421 acctgcatca tgttgatctg gtacaagtcc gtgtgcaatg tcaaactatc atctggatac
     3481 atacttttcc tacttcctta gctagaaacc catgaaaatt ttcaagaact ttcatgtatt
     3541 ccaataaatt agtactatta tatcacattt tagctggatt gagaaaagag taacaagcta
     3601 ttctccactc tccaattcat ccatatcttg ttcaaatttt ttctgagccc attcgccata
     3661 gctcttaaga ccaagattgc caataaagat ccacggaagg taaatgatat aagtaatgac
     3721 ccaagcagac aggtatttaa aattcaaagg attgtgctga taaatttcta tgttgaattg
     3781 ataattctgc aacatcaaaa gagccgtaat agccaaggtt aggaaaaaac aacccaaaat
     3841 cgtaaaatga aaacgactat agtaggtcac tcccagataa cgggcacgat tgaaaaagta
     3901 aaatgtccct atgatgataa cgattagcag catattagaa ttaaaaaggc ttggtgctaa
     3961 tactgaaatg atataagata ggagcgacaa agcaatgcag atatagaaac tttcagagcc
     4021 cgctttattg aacagttgtt cttctctttc gtctagtaat tgataataat aaaatctatt
     4081 tttcatcttc ttcctcccaa aatagttggt ctagggtttt ccctaaacat ctgcaaatag
     4141 actggcagag cgagagactg ggattgtatt ttcccgcctc tattaaacca atagtctggc
     4201 gtgtcacccc gacagcctct gccagttgac cttgtgttaa atcacgctct acccgagcta
     4261 attttaattt taaattttta gccaccttcg tcctccttat agttttaata ctcatctact
     4321 cttaaaaaat ccaaaaccaa cacaagctat cagctattta ttataacccc attaatttgc
     4381 attccagtat tatcttcagc cgaaaatcag tattttaggt tgtttgaaat aaacttccta
     4441 gtttactctt ttatttttat ttagtattag ctttagtaag atcttttaaa cgaattttta
     4501 ttgcttatat ttcttgttta gtttagttgg tttcatatca ctattatata atgcttttgg
     4561 aaatgagcct agtataagtc aaaaacgtgt tgaatagtga aagcaggagc ataccaagat
     4621 gactgatttt ttactgtatt ttaggtatgc aaaataatac taaatgaaac taaaatgaat
     4681 acttcttgct tttcttgaga gtgaaaaagt acaataaaat gattgtagtg attaccacaa
     4741 gatactgact aatagacatc gtatttgaag ccacaaatac aggtaaagaa taaaaaggaa
     4801 gaagttcaat tg
//
LOCUS       DAYYQN010000089         4778 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYYQN010000089 DAYYQN010000000
VERSION     DAYYQN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.090"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            29..733
                     /locus_tag="ACHBRQ_001909"
     CDS             29..733
                     /locus_tag="ACHBRQ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9593515.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    792..879
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            915..1229
                     /gene="rplU"
                     /locus_tag="ACHBRQ_001910"
     CDS             915..1229
                     /gene="rplU"
                     /locus_tag="ACHBRQ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9593516.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1245..1589
                     /locus_tag="ACHBRQ_001911"
     CDS             1245..1589
                     /locus_tag="ACHBRQ_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9593517.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1606..1899
                     /gene="rpmA"
                     /locus_tag="ACHBRQ_001912"
     CDS             1606..1899
                     /gene="rpmA"
                     /locus_tag="ACHBRQ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9593518.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2225..2373)
                     /locus_tag="ACHBRQ_001913"
                     /pseudo
     CDS             complement(2225..2373)
                     /locus_tag="ACHBRQ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            2852..3769
                     /locus_tag="ACHBRQ_001914"
     CDS             2852..3769
                     /locus_tag="ACHBRQ_001914"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFM9593519.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3862..4710)
                     /locus_tag="ACHBRQ_001915"
     CDS             complement(3862..4710)
                     /locus_tag="ACHBRQ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9593520.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1513 a          820 c          974 g         1471 t
ORIGIN      
        1 tggtacaata tttctaagag aaaatacaat gggaggtaaa atgaggttat tacctataag
       61 aaaaatatca cgtcagtcta aaaggttagc actttttttg acgttttgtg ctggatatgt
      121 ggatgcttac acttttattg ttcgcgggaa tacccttgta gctggacaaa ctggaaatgt
      181 tgtctttctt tcagtagaat taattaaaaa taatgtttcg gatgttaggg acaaggttct
      241 caccttgcta gcgtttatga tgggagtctt tttattaacg atttataagg aaaaattgag
      301 aattgtgaaa aaacctattc tgtccttgat tcccttggca atcttatcaa tcattattgc
      361 ttttgtgccg caaactgtgg ataatatcta tctagtgccg cccttggcct tctgtatggg
      421 actggtgaca actgcttttg gagaagtgtc gggtattgcc tataataacg cttttatgac
      481 agggaatatc aaacggacca tgctggcttt tggagattat ttccgaacca agcacactcc
      541 ttttttgcgt gaaggattca tatttgttag cctgcttagt agttttgtcc ttggcgttgt
      601 cttttcagcc tatttgacga ttttctatca tgaaaagacc attcttggtg ttcctattat
      661 gatgagcgtt ttttacctca gcatgctttt tgcctcttgg cagaaaaaag taaaagaaaa
      721 agcttcattt taggtgttat tacttgtaag aaaataggag atatgctata cttgaagagt
      781 tgactatgca cgatcctgtg caaccgcacg aacatcgttg cttgattatt caagtttaag
      841 tggttcgtcc cacgatgcta ggcgagtctt cacaaaaaat cggggaaacc cataaaaatc
      901 ataggaggtg cataatgagc acatacgcaa ttatcaaaac tggcggaaaa caagttaaag
      961 ttgaagttgg tcaagcagtt tacgttgaaa aattgaacgt tgaagctggt caagaagtta
     1021 cttttaacga agttgttctt gttggtggtg aaaacactgt tgtcggaact ccacttgttg
     1081 ctggagctac tgtagttgga actgttgaaa aacaaggaaa acaaaagaaa gtggttactt
     1141 acaagtacaa acctaaaaaa ggtagccacc gtaaacaagg tcaccgtcaa ccatatacaa
     1201 aagttgtcat caacgcaatc aacgcttaat tttaaggaga acacatgata caggcagtct
     1261 ttgagagagc cgaagatggc gagctgagga gtgcggaaat tactggacac gccgagagtg
     1321 gcgaatacgg cttagatgtc gtgtgtgcat cggtttctac gcttgccatt aactttatca
     1381 attctattga gaaatttgca ggctatgaac caatcctaga attaaacgaa gatgaaggtg
     1441 gctatctgat ggttgaaata ccaaaagatc ttccttcaca ccagagagaa atgacccagt
     1501 tattctttga atcatttttc ttaggtatgg caaacttatc ggagaactat tctgagttcg
     1561 tccaaaccag agttatcaca gaaaactaac acggaggaaa acattatgtt aaaaatgact
     1621 cttaacaact tgcaactttt cgcccacaaa aaaggtggag gttctacatc aaacggacgt
     1681 gattcacaag caaaacgtct tggagctaaa gcagctgacg gacaaactgt aacaggtgga
     1741 tcaatccttt accgtcaacg tggtacacac atctatccag gtgtaaacgt tggtcgtggt
     1801 ggagacgata ctttgttcgc taaagttgaa ggcgtagtac gctttgaacg taaaggacgc
     1861 gataaaaaac aagtttctgt ttacccaatc gcaaaataaa aaggttcatt gaacctttta
     1921 tcccgaacct tgaaatgtaa aggtgaggaa gctagaaaca gcttaaaata aggctttccg
     1981 agttttcgga gagcctgttt ttttattttg atacccatac tttttaatat gtatagtgga
     2041 ttgatactct tcgaaaatca aattcaaacc acgtcaattt caccttgccg tactcaagta
     2101 cagcctgcgg ctaacttcct agtttgcact ttaattttca ttgagtatga atctggaata
     2161 gaacactgtt aaaaattgat atgagtttcc tactcgattt cttcagattt tatttcaatt
     2221 gactataaga actgctatct ggtttcaaat attggcttgt cgtcccttca cttgtctgaa
     2281 tggtttgagc cgttccttct gataagggaa aagatattct ttagctgaaa cggtctgaga
     2341 atccaagcta acagatggag agaaaaatag cccagtctga agcacactag atgggagttg
     2401 ttggaaagca gtcatctagt ttttctttgc tgttttggct tttttctttg tcatcaggga
     2461 aagtgacttg cttcaaatca gggagtttag gaaattcttc aaacatcttg cccaagttgt
     2521 ctcattttgt aaattttaca gccataggtg tacctcgatt ctaaattcta cctttttggc
     2581 accaattagt aatttaaagt tctcaggagt tagttgcatt gctttttcat acataacata
     2641 gtatgacact ttatggttac cgaacaagtc taatcactcc ttgcaaaatt ttgaagatgt
     2701 cctacaagtt tttgagaaga atattgcaaa gttcatagtt aagcaacgat agaataaagt
     2761 gctttttgaa aacaggtaca cttcatgtga aaaaaacaac atttcagatt ttctgttcca
     2821 gaaatagcta tcgctatgat ataattattt tatgattatt actattccta taaaaaacca
     2881 aaaagatatt ggcacaccat ctgattcagt cgttgttctc ggctattttg atggcataca
     2941 taaggggcat caagaattat ttcgtgttgc caacaaggct gcgagaaagg atttattgcc
     3001 tatcgtcgtt atgaccttta atgaatctcc aaagatcgct ttagagcctt atcatccaga
     3061 tttgtttttg catattttga accctgctga acgtgaaaga aaattaaagc gcgaaggtgt
     3121 agaagaatta tatctccttg attttagtag tcaattcgct agtctcactg cacaagaatt
     3181 ctttgcaact tatatcaagg ctatgaatgc caaaattatt gttgcaggtt ttgattatac
     3241 atttggttct gacaaaaaaa cagcagaaga tttaaaggat tactttgatg gagaagttat
     3301 cattgttcca cctgtagaag atgagaaagg aaagattagt tcaactcgta tccgtcaagc
     3361 tattttagat ggaaatgtga aagaagcagg aaaacttttg ggggcaccgc ttccatcaag
     3421 aggtatggtg gttcatggta atgctcgtgg tcgtacaatt ggttatccga cagcgaattt
     3481 agtgctttta gaccgtactt atatgccagc agatggcgtt tatgtcgttg atgttgagat
     3541 tcaaagacag aagtatcgtg ctatggctag tgtcgggaaa aatgtgacct ttgatggaga
     3601 agaagcacgt tttgaagtca atatttttga ttttaatcaa gatatttatg gggaaaccgt
     3661 catggtttat tggcttgatc gcattcgtga tatgaccaaa tttgactcag ttgaccaatt
     3721 agtggatcag ttaaaggctg atgaagaagt aactcggaat tggtcttaag agcttgagta
     3781 aataaaacaa aaaagaggtt gtctgtaacc caaaagatag atgatttagt ctaacttttg
     3841 aggtcacgac attacctctt tttattcttt ttcaaaggtg aagccttctc ctaggatttc
     3901 atgggcttct gtaatagtta taaaggcttg aggatcgatt cgatgaatca tttccttcgt
     3961 tttcacaatt tcatttcttc cgacaataca gtagatgatt ttcaaatttt ctttactata
     4021 gtagccttga ccagaaataa aagtaacacc tcttccgagg tcatcattaa tcgccttagc
     4081 aagttggtca ggacgttttg tgataatcat aaagcctttg ccggcatatc ctccttcacc
     4141 aatcaaatca ataacacgag aaacaataaa atcaaacaaa agcgtgtagg aaaccaatct
     4201 caaatccttg aagattagga gaatcaacat gagaatacaa aaatctaaga taaagagcag
     4261 ttttcctatg gatatatgag tgtatttgtt gagaatacga gctagaatat cagttccgcc
     4321 agttgtacct ccagcattaa aaataattcc aaggccaatt cccaatagga ttcccgctat
     4381 aagggctgtg attagtaaat caccttgaag atcaatatga aggggaatat gctcaaaaaa
     4441 agctaaccag gcggacaaag ctaaggttcc tagtaaacta gaatagaggg atttggctcc
     4501 aaagatcttc caagctagga tgaaaagggg aatattaatc agcaggttca tgagggaaac
     4561 agggatttta aaaagataaa aggtgatgag ggtaatgcct gtcgcccctc cttcaaagag
     4621 atgatgggga actacaaaat aagtcagtcc aaaagcataa atagcagcac ctagtaaaat
     4681 ggtaaaaatg gggtaaattt ttttaatcat aggacacctc ttttcttata agttgattat
     4741 atcaaatttt acggaaaatt tgattgactc cattagga
//
LOCUS       DAYYQN010000090         4775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYYQN010000090 DAYYQN010000000
VERSION     DAYYQN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.091"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            185..1730
                     /locus_tag="ACHBRQ_001916"
     rRNA            185..1730
                     /locus_tag="ACHBRQ_001916"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1782..1854
                     /locus_tag="ACHBRQ_001917"
     tRNA            1782..1854
                     /locus_tag="ACHBRQ_001917"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1815..1817,aa:Ala,seq:tgc)
     gene            1979..>4775
                     /locus_tag="ACHBRQ_001918"
     rRNA            1979..>4775
                     /locus_tag="ACHBRQ_001918"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1333 a          980 c         1413 g         1049 t
ORIGIN      
        1 gttgacaagt gaaagtggct gtgatatact aatatagttg tcgcttgaga gaagcaagtg
       61 acaaagacct ttgaaaactg aacaagacga accaatgtgc agggcgctac aacataagtt
      121 gtagtactga acaatgaaaa aacaataaat ctgtcagtga cagaaatgag taagaactca
      181 aacttttaat gagagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat
      241 gcaagtagaa cgctgaagga ggagcttgct tctctggatg agttgcgaac gggtgagtaa
      301 cgcgtaggta acctgcctgg tagcggggga taactattgg aaacgatagc taataccgca
      361 taagagtgga tgttgcatga catttgctta aaaggtgcac ttgcatcact accagatgga
      421 cctgcgttgt attagctagt tggtggggta acggctcacc aaggcgacga tacatagccg
      481 acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc tacgggaggc
      541 agcagtaggg aatcttcggc aatggacgga agtctgaccg agcaacgccg cgtgagtgaa
      601 gaaggttttc ggatcgtaaa gctctgttgt aagagaagaa cgagtgtgag agtggaaagt
      661 tcacactgtg acggtatctt accagaaagg gacggctaac tacgtgccag cagccgcggt
      721 aatacgtagg tcccgagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggtta
      781 gataagtctg aagttaaagg ctgtggctta accatagtag gctttggaaa ctgtttaact
      841 tgagtgcaag aggggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga
      901 ggaacaccgg tggcgaaagc ggctctctgg cttgtaactg acgctgaggc tcgaaagcgt
      961 ggggagcaaa caggattaga taccctggta gtccacgctg taaacgatga gtgctaggtg
     1021 ttagaccctt tccggggttt agtgccgtag ctaacgcatt aagcactccg cctggggagt
     1081 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg
     1141 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatccct ctgaccgctc
     1201 tagagataga gctttccttc gggacagagg tgacaggtgg tgcatggttg tcgtcagctc
     1261 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccctattgt tagttgccat
     1321 cattcagttg ggcactctag cgagactgcc ggtaataaac cggaggaagg tggggatgac
     1381 gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg ctggtacaac
     1441 gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc agtctcagtt cggattgtag
     1501 gctgcaactc gcctacatga agtcggaatc gctagtaatc gcggatcagc acgccgcggt
     1561 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg
     1621 aagtcggtga ggtaaccgta aggagccagc cgcctaaggt gggatagatg attggggtga
     1681 agtcgtaaca aggtagccgt atcggaaggt gcggctggat cacctccttt ctaaggataa
     1741 ggaactgcgc attggtcttg tttagtcttg agaggtcttg tggggcctta gctcagctgg
     1801 gagagcgcct gctttgcacg caggaggtca gcggttcgat cccgctaggc tccattggtg
     1861 agagatcacc aagtaatgca cattgaaaat tgaatatcta tatcaaatag taacaagaaa
     1921 ataaaccgaa aacgctgtag tattaataag agtttatgac tgaaaggtca gaaaataagg
     1981 ttaagttaat aagggcgcac ggtggatgcc ttggcactag gagccgacga aggacgtgac
     2041 aaacgacgat atgccttggg tagctgtaag taagcgatga tccagggatt tccgaatggg
     2101 ggaacccaac aggtaatacc tgttacctac atctgttaag gatgtgagga ggaagacgca
     2161 gtgaactgaa acatctaagt agctgcagga agagaaagca aaagcgattg ccttagtagc
     2221 ggcgagcgaa acggcagaag ggcaaaccga agagtttact cttcggggtt gtaggactgc
     2281 aatgtggact caaagattat agaagaatga tttgggaaga tcagccaaag agagtaatag
     2341 cctcgtattt aaaatagtct ttgtacttag cagtatcctg agtacggcgg gacacgtgaa
     2401 atcccgtcgg aatctgggag gaccatctcc caaccctaaa tactccctag tgaccgatag
     2461 tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga aatagaacct
     2521 gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg ccttttgtag
     2581 aatgaaccgg cgagttacgt tatgatgcga ggttaagttg aagagacgga gccgtaggga
     2641 aaccgagtct gaatagggcg ccttagtatc atgacgtaga cccgaaacca tgtgacctac
     2701 ccatgagcag gttgaaggtg cggtaagacg cactggagga ccgaaccagg gcacgttgaa
     2761 aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt
     2821 tctctccgaa atagctttag ggctagcgtc gacattagag attcttggag gtagagcact
     2881 gtttgggtga ggggtccatc ccggattacc aatctcagat aaactccgaa tgccaatgaa
     2941 ttatggtcgg cagtcagact gcgagtgcta agatccgtag tcgaaaggga aacagcccag
     3001 accaccagct aaggtcccaa aataattgtt aagtggaaaa ggatgtgggg ttgcacagac
     3061 aactaggatg ttagcttaga agcagctatt cattcaaaga gtgcgtaata gctcactagt
     3121 cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacaa tttaccgaag ctgtggatac
     3181 ctttataggt atggtaggag agcgttctat gtgtgatgaa ggtataccgt gaggagtgct
     3241 ggaacgcata gaagtgagaa tgccggtatg agtagcgaaa gacaggtgag aatcctgtcc
     3301 accgtaagac taaggtttcc aggggaaggc tcgtccgccc tgggttagtc gggacctaag
     3361 gagagaccga aaggtgtatc cgatggacaa caggttgata ttcctgtact agagtatgta
     3421 gtgatggagg gacgcagtag gctaactaaa gcagacgatt ggaagagtct gtctaagcag
     3481 tgaggtgtga attgagtcaa atgcttaatt ctataacatt gagctgtgat ggggagcgaa
     3541 gtttagtagc gaagttagtg acgtcacact gccaagaaaa gcttctagcg tttaaacata
     3601 ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag gtgagcgaga
     3661 gaactctcgt taaggaactc ggcaaaatga ccccgtaact tcgggagaag gggtgctgac
     3721 ttaaagtcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac agctctctgc
     3781 taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg ttaagaggag
     3841 tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg taactataac
     3901 ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa aggcgtaatg
     3961 atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga agatgcaggt
     4021 tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata ttgagtgtct
     4081 gtaccacatg tacaggatag gtaggagtct aagagatcgg gacgccagtt tcgaaggaga
     4141 cgctgttggg atactaccct tgtgttatgg ccactctaac ccagataggt gatccctatc
     4201 ggagacagtg tctgacgggc agtttgactg gggcggtcgc ctcctaaaag gtaacggagg
     4261 cgcccaaagg ttccctcaga atggttggaa atcattcgca gagtgtaaag gtataaggga
     4321 gcttgactgc gagagctaca actcgagcag ggacgaaagt cgggcttagt gatccggtgg
     4381 ttccgtatgg aagggccatc gctcaacgga taaaagctac cctggggata acaggcttat
     4441 ctcccccaag agttcacatc gacggggagg tttggcacct cgatgtcggc tcgtcgcatc
     4501 ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg cacgcgagct
     4561 gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag gaaatttgag
     4621 aggatctgct cctagtacga gaggaccaga gtggacttac cgctggtgta ccagttgtct
     4681 tgccaaaggc atcgctgggt agctatgtag ggaagggata aacgctgaaa gcatctaagt
     4741 gtgaaaccca cctcaagatg agatttccca tgatt
//
LOCUS       DAYYQN010000091         4658 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYYQN010000091 DAYYQN010000000
VERSION     DAYYQN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.092"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(293..1021)
                     /locus_tag="ACHBRQ_001919"
     CDS             complement(293..1021)
                     /locus_tag="ACHBRQ_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9593521.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1179..2588
                     /locus_tag="ACHBRQ_001920"
     CDS             1179..2588
                     /locus_tag="ACHBRQ_001920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9593522.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2606..3901
                     /locus_tag="ACHBRQ_001921"
     CDS             2606..3901
                     /locus_tag="ACHBRQ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9593523.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3906..4214
                     /locus_tag="ACHBRQ_001922"
     CDS             3906..4214
                     /locus_tag="ACHBRQ_001922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9593524.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4211..4519
                     /locus_tag="ACHBRQ_001923"
     CDS             4211..4519
                     /locus_tag="ACHBRQ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9593525.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1575 a          796 c          859 g         1428 t
ORIGIN      
        1 ctatatgtgt tacaagaaaa gactcaaaaa cttctcgtca gtgtaagaat atttgaaaca
       61 ctatttaaaa ctaaataact ataagaagta gatcaggtga acaatttcag tacctactct
      121 ataagctata cgaagatatt tttcaacatg atggataaaa cgatcactct tttaaagcca
      181 tcttatttag aaaagagaaa ccatttaagc atagatggga ccttcttgcc ctgttttaaa
      241 tttttcttaa actagagtct gtaaaagatt ggcttcattt tcacattttc tcctaggagg
      301 aatgtcgtaa tgcaaaagaa ttaaattcaa ataaatcata gcgatgacga ctaatcgagt
      361 actcaaacgg agttccgtta tccaaataag ccacttgttc tacttcaaat accggttccg
      421 ttggaaggag ctgcaagtaa tgttgctcat tttctgaact agaagaagct cgtaagattt
      481 ttgtagcact ggcaatatgc aatcctaact ttccttcaat gtaattgtaa atcgattttt
      541 gtaaaatatc ttcagtaata cctggaataa catcggtact catataagtt tgttccagaa
      601 cataaggttt accgtcaata atacgcaggc ggtaaatatt ataaactgga ctctgcaaag
      661 cgacctgtag tttttctgct aaaaattcat ttgcaaattc taatttgaag tgaataatcc
      721 tcgagtcaat tgtagaatga gcatcttcag atatttttgt cagtccccgg atatctcttt
      781 ctggaacgat taattttctt tttgagctgc cacgagagag aacaaaggtt ccctgcccac
      841 gttttctaaa aattaagccc tcagaaacta acaagtctaa agcttttttg atcgtcattc
      901 gactacaggc aaactgttta gctaatgtca tttcatcagg taattgttcg ttcatggtat
      961 atttattaca aagaatcttt tcacgaatct cattgtaaat aaataaatat tttgtactca
     1021 tttaatctgg ttctctctat aatattctaa tagtagtata acaaagttca agaaatgagg
     1081 caaacacaag atatgtatca acaattttta aaaatgtcta gacaaatttt aaaattatgc
     1141 tatactttat gtatagaata atagaaagga ggttgagcat gaaaaaaaag ttagtatttc
     1201 ctaatctgtt ttggtgggga gcagcttcta gcggacctca gacagaaggt caatatggaa
     1261 aagtacatga aaatgtgatg gactactggt tcaaaacgca tccagaagat tttttcgata
     1321 atgtcggacc tcttgtagcc agtaactttt ttcatactta caccgaagat ttccacttga
     1381 tgaaggaaat tggagttaat tctttccgca cttccatcca atggagtcga ctcatcaaga
     1441 atttagagac aggtgagcct gatccaaaag gtattgcttt ctacaatgcc atcattgaag
     1501 aagctaaaaa gaaccagatg gatcttgtga tgaatttaca tcattttgat ttaccagtgg
     1561 aacttcttca aaaatacggt ggttgggaaa gcaaacatgt agtggagtta ttcgtgaagt
     1621 ttgccaagac tgctttcaca tgctttggag ataaggttca ttactggaca actttcaatg
     1681 agccaatggt cattccagaa gcaggatact tatatgcttt ccattatcca aatctaaaag
     1741 gaaagggaaa agaggccgta caagtcatct ataatctaaa ccttgctagt gcaaaagtga
     1801 ttcaactata tcgctcatta gaacttgatg gaaagattgg gattatttta aacttgacac
     1861 ctgcttatcc aagaagtaat tctccagaag acttagaagc aagtcgattt acagatgact
     1921 tctttaacaa agtcttcttg aatccagctg ttaaaggaac tttcccagaa agattggtaa
     1981 aacagctaga gagagatggc gtgttatgga gtcataccga aaaagagctt caactgatga
     2041 aatcaaatac ggttgatttt cttggagtaa actactacca tccaaaacgt gttcaagcac
     2101 aagcaaatcc tgaggaatat cagacgccct ggatgccaga ccaatacttc aaagagtatg
     2161 aatggccgga gcgtcgcatg aatccatatc gtggttggga aatttttccg aaatccattt
     2221 atgatattgc tatgattgtg aaggaagaat atggtaatat cccatggttt atcagtgaaa
     2281 acggaatggg tgttgaaaac gaagcacggt ttatcgatga aaatggagtt atcgatgacg
     2341 tgtatcgtat tgaattttat gaagaacatt taagatggct acataaagcc attgaagagg
     2401 gaagtcactg ttttggatac cacgcttgga ccgcatttga ttgctggtct tggaataatg
     2461 catataagaa tcgttacgga tttatctccg ttgatttaga aacgcaaaag agaaccatca
     2521 agagctcagg aagatggtat cgcaaagtaa gtgacaataa cggttttgaa gtagaaattg
     2581 aggagtaaag agaggagata ttcatatgaa aaactcaaaa tttatagacc aatttgccac
     2641 ctttgctggt aaactaggga accaaattca tttaaaaacc ctaagagatg cattcgtaac
     2701 agtaatgcca ttatatattt tggcaggttt aatcgttctt ttgaacaaca cggtatttaa
     2761 gtggattttc caaggggata cattaacaag attccaatat tggggaataa caattgcaaa
     2821 cggtacttta agtatttcag gtatgattat tgctgtaatg gttggttatt tcttagctaa
     2881 aaacagagat ttcgaaaacc cgttagcagc atcaatgcta tcattagttt ctttaattgt
     2941 gatgatgcca aatacagttt ctgtagttcc tgacggagca aaagatgctg taaacatttc
     3001 aggtgttctt tcattcaaca acacaggtac aggcgcaatg ttcgccgggg ttatcgtagc
     3061 gattattgca acagaattat tcattgaatt atcaaatgtt aaagctttac aaatgaacct
     3121 tggtgaaaat attccaccag ctgttaatag atcatttagc gtattacttc cagtcatgac
     3181 cgtcatctcc ttatttgggg ttgtttcagc attattattc aatataactg gaatgaactt
     3241 aatctcaatc attacaatct ttattcaaga accaattcgt catattggta caagcttaat
     3301 cggggtcatt attatttact ctttaggaaa tatgttatgg ctatttggta ttcaccaagc
     3361 agttatttac agtgccatcc tagaaccatt actattaatt aacattactg aaaacatcac
     3421 tgcagcaaat aatggacaag ccattccaca catcatcaac ctatcacaaa tacaaacatt
     3481 cgctttaatg ggtggtagtg gatctacatt atgtttatta atagcaacat tcttagtgag
     3541 tcgcaatgct gtctctaaaa acgtggctaa attatctttt ggacctggta tcttcaatat
     3601 caatgaacca gtattattcg gttacccaat cgtttataac atttcattag ctattccatt
     3661 tatcacagtt ccagtccttg gtattttaat cagctactta gcaacagtta caggattcat
     3721 gagtcctgca tttatacaag ttccttggac tacaccagta ttcttaaatg catggttagc
     3781 aacagcaggg gacgtgagag cagttctagt tcaattcatc atctttgcac ttggagttct
     3841 tctatacatt ccatttatca aagttaatga caaagttgtt gaacaagaaa tggaaggtta
     3901 agattatgaa acaaatttta ttagtatgta atgcgggaat gtcgacaagt atgcttgtta
     3961 aaaaaatgca acaaagtgcg actgaacgtg ggattgaaat ctctatccaa gcaaaatcaa
     4021 tgacagaagc taaaaagaat atccatgaag cagatgtcat tctaattggg ccacaaattc
     4081 gttatgagtt acttgctgtg aaagaaattg caggaaatat ccctgtagat accattgata
     4141 tgcgggatta cggaatgatg aatggagcaa aagttctgga acaagcactt gcatggatag
     4201 gagaaataag atgacagaag aaatggaata tatctgtttt cagctcattg ccaatagtgg
     4261 agcagccaaa tcatccttta ttgaagccat ccaactagct aaggcaggaa atttaaaaga
     4321 agcgaaaatt aaagttgaag aagcggaaga ctccttagta gaagcgcaca agattcactc
     4381 taatctcata caaaaagaag ctactggaga aaaaatagga ttctccttgc tatttatgca
     4441 tgcagaagac caaatggctt caacagagat tattcagttg ctatctaaag aattcatcga
     4501 tctctatcaa aataaatagg aacactgtca ttcccatctg tctttattaa tgacgactac
     4561 attgtaggaa cctccaaaag ttggacaaaa tgattgaaag gattggatcc tgcattacac
     4621 aagatccaat ccttttcttt tactctagtt tttataat
//
LOCUS       DAYYQN010000092         4550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYYQN010000092 DAYYQN010000000
VERSION     DAYYQN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.093"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            257..2590
                     /gene="hsdR"
                     /locus_tag="ACHBRQ_001924"
     CDS             257..2590
                     /gene="hsdR"
                     /locus_tag="ACHBRQ_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFM9593526.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMNTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTIAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2603..4066
                     /locus_tag="ACHBRQ_001925"
     CDS             2603..4066
                     /locus_tag="ACHBRQ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFM9593527.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4066..>4550
                     /locus_tag="ACHBRQ_001926"
     CDS             4066..>4550
                     /locus_tag="ACHBRQ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9593528.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     N"
BASE COUNT         1596 a          686 c          939 g         1329 t
ORIGIN      
        1 gtgattattt atttttgaaa aatagctcac cagcccgact gaaagtgctt atagaatgat
       61 aataagtcgc ctgccgaaaa cagcggaaaa tagcggtgtt atgcggagat aatctgacgc
      121 gatgcgaaag tatattgcat acttattttc aacaatttag cagagtattt ttataagtgt
      181 gatataatag aagtataatt tgttctgata gtttatttta tggagaagta gatttttaga
      241 atgcggaggg ttcaatatgg ttgagtttat aaagtctaag aaagaaatga gtgaggagga
      301 tattaaagca aatttcatca ctcctgctat tgtatccaaa ggatggaaaa atggtgagca
      361 tatcgcttac gaagaatact tcactgatgg tcgaattgag gttagaggag ataaggctcg
      421 tcgtaaagaa ggaaaaaaat cagactattc actgtattac caatttggaa ctcgaattgc
      481 aattgttgag gcaaaggata ataaacacag cgttcgagca ggattacaac aagctattga
      541 atatggagag attttagatg ttccatttgt ttattcttcg aatggtgatg gctttattga
      601 acacgaccgt atcacgagag aagaacgtga gctggagtta gacgaattcc ctactcgtga
      661 agaattattt tctcgtatga cgaaggaaaa aggattgacg tacgaaatta cagaagctat
      721 ctcaactcca tactatacag acgccttctc aatgaacacg ccacgctatt atcagcaaat
      781 agctatcaac cgtactattg aaacagttgc cagaggacaa aaacgagtaa tgtttgtgat
      841 ggcaacagga acggggaaaa cgttcatggc ttttcaaatt attcatcgcc ttcgaaaagc
      901 tggtttggct aaacgagttt tattcttagc agatagaaac atcttagtag accaaacgat
      961 agctgaagac tttaagccat tcgaaaaggt aatgacgaaa attacaccaa aacttttgac
     1021 tgctcctgaa aaattaaatt cttttgaaat ttatctaggg ctttatcagc aactaactgg
     1081 tgaagatgga actgaaacac attatcaaaa atttgacaaa gacttctttg atttaatcgt
     1141 aattgatgaa gcgcaccgtg gttcagctaa ggaaaacagt aactggcgta agataattga
     1201 ttatttcagt tctgcgacac agattgggat gaccgctact cctaaagaaa ccaagaatgc
     1261 ttccaatacg gaatactttg gtgagccaat ctatacttat agtttaaaac agggaatcga
     1321 ggatggtttt ttggctccat atcgtgttat gagagttaat ttagatgtag atgtggatgg
     1381 ttatcgtcca gaaactggaa aagttgatgc taacggacaa ttaatagaag ataggtacta
     1441 cggcaggaaa gattttgata aaaccattgt cattgatgat agaacgcaaa gagttgccaa
     1501 gtttgtttct gattatatga agcaaaacaa tgcacgattt gataaaacaa ttgttttttg
     1561 tgttgatatt gaccatgccg agcgaatgcg tgctgcactt gtaaaagaga atctagactt
     1621 agtccaagaa gactatcgtt atgtcatgca agtaactggt gacaacgctg aaggaaaagc
     1681 tcaactggat aactttatgg atgtcaattc taattttccc gctattgtaa caacgtctaa
     1741 attattaacg acaggagtta atgctaaaac atgtcgtttg attgttttag actctaatat
     1801 ccaatccatg actgaattta aacaaattat tggtcgtggc acacgtcttt atcctcaaaa
     1861 ggggaaagaa ttttttacga ttattgattt tcgaaatgtt accaatttgt ttgctgaccc
     1921 tgattttgat ggtgatccag tgaaggtgct ggaaacaggt gcgaaaacag tcagtggttc
     1981 tacgcccggt ttcgtaagtg aggaaggtga cccagtagaa aaatatatcg ttacagacaa
     2041 gcaggttacc attcttaatt ctactgttca agtattggat gaaaacggga aactgattac
     2101 cgaaagcctg accgactaca ctcggaagaa tatcttaggt agctacgcca ctttgaacga
     2161 ttttatcaca gtttggcata cggcggataa aaagaagctt atcttggacg aactttataa
     2221 aaaaggagtt tatctagatg ctattcgaga gtcggaggga atatcagaac aagaaatcga
     2281 tgattttgat ttactcctaa aacttgccta tggtcaaaaa gaattaacca aaacggaacg
     2341 tatcaataag ctcaaacaaa gcggatattt atataaatat agtgaggaag cgcgtgctgt
     2401 tttggaaatt ttactgaaca aatacatgga taaaggtatt ggagaactcg aaagcattga
     2461 aacattaaaa cttccagaat ttcagatata tggtggaacc ttcaaaatca tcaatactta
     2521 ttttggagat aaaaaacgat atttacaagc aattaaagaa ttggagcaag agctatttac
     2581 agtagcttaa tgaaaggaaa gtatgtcaat tacatcattt gtaaaaagaa ttcaagatat
     2641 cactcgaaac gatgctggtg ttaatggtga tgctcaacgt attgagcaaa tgtcttggtt
     2701 attattctta aaaatttatg atagccgtga aatggtttgg gaattagaag aagacgagta
     2761 tgagtcaatt atcccagagg aattaaaatg gcgaaattgg gctcatgctc aaaatgggga
     2821 acgggtattg acaggcgatg aattacttga ttttgtcaat aacaagttat tcaaagagtt
     2881 gaaagagctt gaaataactt caattatgcc tattcgaaaa acgattgtta aatcagcttt
     2941 tgaagatgcg aacaactata tgaaaaatgg cgtcttgtta cgccaagtca tcaatgttat
     3001 tgatgaagtt gatttcaata gccctgaaga tcgtcattcg tttaatgata tttacgaaaa
     3061 aattcttaaa gatattcaaa atgctgggaa ctcaggagaa ttttatacgc cacgtgcagc
     3121 gactgatttt attgccgaag ttcttaaccc aaaacttgga gaatcaatgg cagaccttgc
     3181 ttgcggaaca ggaggcttct tgacttcgac tctgaaccgt ttaagtagtc aacgtaaaac
     3241 tagtgaagat accaaaaaat ataatacagc tgtttttggt attgaaaaga aagcatttcc
     3301 tcatctttta gcagttacaa atctgtttct tcacgaaatt gatgacccta aaattgttca
     3361 tggaaatact ttggagaaaa atgttcgtga atatacggat gatgaaaaat ttgacattat
     3421 tatgatgaat ccaccttttg gagggtcaga attagaaaca ataaaaaata actttccagc
     3481 agaattacgg agttctgaaa cagctgattt atttatggct gtcattatgt atcgtttgaa
     3541 agaaaatggt cgtgttggag ttattttacc tgatggtttt ctatttggtg aaggtgtaaa
     3601 aactcgcttg aaacaaaaac tggtagatga gttcaacttg catacgatta ttaggttgcc
     3661 tcatagtgtc tttgcaccgt atacaggaat ccatacgaac attcttttct ttgataaaac
     3721 aaagaaaaca gaagaaactt ggttttatcg tttagatatg ccagatggtt ataaaaattt
     3781 ctcgaaaact aagccgatga agtcagaaca cttcaatcct gttcgtgact ggtgggaaaa
     3841 tcgtgaagag attctggaag gtaagttcta caaatctaaa tcatttacac ctagtgaatt
     3901 ggctgagttg aattataatt tagaccagtg tggctttcca aaagaggaag aggaaatctt
     3961 aaatcccttt gagttgattc agaattatca agcggaaaga gcaactttaa atcataagat
     4021 tgataatgta ttagctgata ttttgcagtt gttggaggac aaataatgac accagaacaa
     4081 cttaaagcaa gtattctcca aagagcgatg gaagggaaat tagtgccgca aaatcccaat
     4141 gacgaacctg caagtgaatt attaaagaga attaaagctg aaaaagaaaa acttatcagt
     4201 gaaggaaaaa tcaaacgaga taaaaaggaa actgagatat ttcgtggtga tgatgggaaa
     4261 cattatggga agtttgctga tggaagcact caagaaattg atgttcctta tgatattcct
     4321 gatacttggg agtgggtgag gataaaatca atttattgga attttgggca aaataagcca
     4381 gagaaatcct ttaggtatat agatacgtct agtattgata gaaaaaagaa cataatcaac
     4441 tacaaaaatc tacaatatct ttcacctgaa caagcgcctt cccgtgctag aaaattagtt
     4501 tcgcagaata gtgtcttatt ttcaacagtt agaccatatc taaaaaatat
//
LOCUS       DAYYQN010000093         4393 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYYQN010000093 DAYYQN010000000
VERSION     DAYYQN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.094"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(254..670)
                     /locus_tag="ACHBRQ_001927"
     CDS             complement(254..670)
                     /locus_tag="ACHBRQ_001927"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9593529.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(682..1377)
                     /locus_tag="ACHBRQ_001928"
     CDS             complement(682..1377)
                     /locus_tag="ACHBRQ_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9593530.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1571..4072)
                     /gene="leuS"
                     /locus_tag="ACHBRQ_001929"
     CDS             complement(1571..4072)
                     /gene="leuS"
                     /locus_tag="ACHBRQ_001929"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9593531.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
BASE COUNT         1235 a         1009 c          832 g         1317 t
ORIGIN      
        1 ctttatttaa taactacatg atacaagacg aaacttaaaa ctagcttaac ttttctaaaa
       61 ttttactatt ttgcaaaaaa tttctatcac cagcacctca ccacgcaaaa aagccacctg
      121 attgggtggc ttcattagga gattgtgatg aaaaaagttt aggattttat taaataaagt
      181 taggaggtct ttatttaatg actatataat actagaccat ccttaaattt tgcttaagaa
      241 aaaacaagtt ttgttatttt ttacgattca tccaagtcat ccctatacaa ttataggtgg
      301 ataagatttc aaagcccata gaacgataga agcccaaggt tctttctgtc tcttctgtca
      361 ccagctggac ttgataggca tctttgtaat cctctaaagc ctctttcatc aaggcactac
      421 caattccttg acgctgatag attggtaaaa cgattaaatc ctgaaccaat actgatgaga
      481 atccatctcc aaccaaacga atcaagccca ccacagcatc accatcaagt gccagataaa
      541 ttactaatga atgagataag gcctgctcca gcatctctgg ttgatgggta taatttgtcc
      601 aaccgacagc ctgatagaga tgtaaaacat cctctagctt gacaatttct tgctttttaa
      661 tagtaatcat ctcaacacct cttaaagttc tctcaagctc ttgtactgct gtccattttt
      721 atcaaaattt tcaggacgca accatgtttc caaacgatct ttgacttgag gccagtcctt
      781 atcaatcata gacaaccaat ccgtatctct cgtacgtccc ttataaacca ctgcctgacg
      841 gaaggttcct tcataaataa agctcaaacg ctccgcagct cgtcttgatg gcagatttaa
      901 ggcatcgcat ttccactcgt agcgacgata gtttagctcc tcaaaaacat agcgagctag
      961 gagatactga gcttcagttc ctatccgtgt accccttagc tctggagaaa aagtgactgc
     1021 tccgacttct attactcggt tattctggtc aatgcgcatg agagaaaaag atcccaaagc
     1081 cttaccagtc gccttgtcta tgattgcata gtaaaaacgg tccttacgag ccaacatctg
     1141 atgtaaaagg ctaaccagct cccccatatc tgctactggc tcctgaaaaa ggtaggtcca
     1201 catctcccga ggagtatccg ggccataaac agctagcaaa tcctccgcat gcttttccac
     1261 tgaaagagcc tctatcctag cataacgccc ttctaagaaa tcaatagaag gcagttcacc
     1321 tggtgtataa ccttccattg actccccaat catttgacca tattcattta ctggcatagc
     1381 tttctccttt tttcactttt caaactttat agttgcccat agtataccat actttcataa
     1441 gaagattcaa aaaatcctcc agattctact ctgaaggatt cctaccttat ttcacaacga
     1501 tattaacgag tttagttggc tcaaacagtt tgggaaactg tttgaggttg gaaatcagca
     1561 aataaattgg ttattttaca acaatattaa ccaatttatt tggtacacta atcactttca
     1621 cgatttcctt accgtcaatt tctgctttga ctttttcatc cgctagagca atttcttgca
     1681 attcttcacg tgataggtct ttagcgacca tgagtttggc acggacttta cctttgattt
     1741 ggacaacgat ttcgatttcg tcttcaacca gtttactttc gtcccaagtt ggccaagcta
     1801 cataagagat tgactcacct gtttctgcga ctgtttgcca gagttcttct gccaagtgag
     1861 gtgcaaatgg tgcaatcaat tgaataaagc ctttggcata gtcaacataa agcttatctt
     1921 ccttgttagc agcattgaca aagaccataa gttgggcaat agctgtgttg aatttgagag
     1981 actcaatttg ctcagtaaca gctttgactg tttcgttgta agccttgtca agagcaccat
     2041 tgttttccgc aaggatttct ttacttgtaa tcaaacggta aactcggtca aggaacttac
     2101 ggcttccttc caaaccttct tctgaccaag caatcgaagc atcgagtggt cccataaaca
     2161 tttcataaac acgaagggta tcggcaccgt attgttccac cacatcatct gggttaacaa
     2221 cgttcttgag cgatttagac atcttggctg gcgcttgctc caactcttcc cctgtttcta
     2281 tatgaaagaa ggaaccatca cgtttttcaa ccttgtcggt tgccacaaga gcaccacggt
     2341 ggtcacggta gcttgttccc aaaatcatcc cttggttaaa gagtttttgg aatggttcct
     2401 tagtcggaac aacaccgagg tcatagagga atttatgcca gaaacgagca taaagcaagt
     2461 gaagtacagc atgttccgca ccacccacgt agatatctac tggcaaccat tgtttgagga
     2521 ggtcctcatc agccaatttc tcagtattgt gcgggtcaat atagcggagg tagtaccagc
     2581 ttgaaccagc ccattgtggc atggtgttag tttcacgacg acctttgaca ccatcttcac
     2641 gagtcacttc aagccaatct gtcaagttag ctagtggact ttcaccagta cctgaaggac
     2701 ggatatcctt ggttacaggc aagacaagcg gcaattcagt ttcaggaaca gctgttgaag
     2761 ttccatcttc ccaatgaatg attggaattg gctcacccca gtaacgttga cggctaaaga
     2821 gccagtcgcg gaggcggtag gtaaccttct cctgaccaca gcctttttct tccaagcaag
     2881 ccacaatctt ggcaatagcg tcttctttgt tcaatccatc taggaagtct gaattgacat
     2941 gcaggccatc ctctgtgtag gcagcttctt cgacatttcc accttcaagt acttcgacga
     3001 ttggaaggtc aaattgtttg gcaaattccc agtcacgttg gtcgtgggca ggcacagcca
     3061 taaccgcacc tgttccataa ctagcaagaa catagtccgc aatccagatt ggcatttcct
     3121 taccattgac agggttgatg gcataggcac ctgtccaaac accagttttt tccttagcaa
     3181 ggtctgtacg aaccaagtca gacttaaggc tggcttggtg tttatagtct gctacagctt
     3241 ctgcttgctc tgaacttgtg atagcgtcta ctaattcatg ttcaggagcc aagacagtga
     3301 aagtcgcacc gaaaagtgtg tccggacgag tagtaaagac tgtaaattcc ttgtctgttc
     3361 cttttacttt gaaagttaca ttggcaccag ttgatttacc aatccagttg cgttgcatat
     3421 ccttgataga ctctgaccaa tctagttcat ctaagtcatt gagcaagcgc tctgcgtaag
     3481 ccgtgatttt gagcatccat tggcgcattg gtttgcggac aactggatag cctccacgct
     3541 cagaagttcc gtcaggaagc acttcttcat tggcaatggc agttcccaat tcctcaaccc
     3601 agtttactgg cacttcagct tcataggcca agcctttttc gtaaagcttg gtgaaaatcc
     3661 attgagtcca cttgtagtag tttggatctg ttgtgttgac ttcacgatcc cagtcataag
     3721 aaaatccaag cgcattgatt tgacgtttga agttggcaat gttttccgct gtaaattctg
     3781 ctgggtcatt accagtatcc atagcgtatt gctctgcagg caaaccaaaa gcatcccaac
     3841 ccattgggtg aagaacattg tagccttgcg cacgtttgta acgactgagg atatcggttg
     3901 cagtataacc ttctgggtgt cctacgtgca gaccagctcc agacggataa gggaacatat
     3961 caagcgcata aaacttaggt tttgatgcat ctgttcctgt cttaaatgta tgatgttctg
     4021 cccagtagcc ctgccactta ggctcaattt ctttatgatt ataaaaactc atggtctctc
     4081 tccaattttg tgatgttact attataccat ttttgaggga atttgaaatg tgtttaaagg
     4141 aaaaactgta tactgtatct cattaatttt gatgactatg aattgctcta tctcccactt
     4201 tttatcccat tgttatgcag taaatcaagg agtaagtcaa gaagcttatc tcgtatagca
     4261 caaaatactt attttagacc gaagataaaa ctggaatgct aattaatgta ttcacaataa
     4321 atagccgaca acctatgtcg ctttttgatt tatgaagaac gtatagtgca ctgaatctga
     4381 aatagtacga aac
//
LOCUS       DAYYQN010000094         4364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYYQN010000094 DAYYQN010000000
VERSION     DAYYQN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.095"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(47..1135)
                     /locus_tag="ACHBRQ_001930"
     CDS             complement(47..1135)
                     /locus_tag="ACHBRQ_001930"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HFM9593532.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1153..1821)
                     /locus_tag="ACHBRQ_001931"
     CDS             complement(1153..1821)
                     /locus_tag="ACHBRQ_001931"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM9593533.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(1840..4278)
                     /locus_tag="ACHBRQ_001932"
     CDS             complement(1840..4278)
                     /locus_tag="ACHBRQ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFM9593534.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
BASE COUNT         1370 a          911 c          666 g         1417 t
ORIGIN      
        1 aggatcaata aaagactacc atttggaaaa cagtagtcct tattgtttat tttgaattta
       61 cataggcatc tacagcgata atagcttgag caacatctga aggcgaaatc ttaaacggca
      121 tctgatgaat tgtctcaccc tccatagttg cttgtttacc aacttttatt aaatcatcat
      181 atccaacttg atccaaatgc atttctttta gagttgttgg cataccaatt tttttgtaaa
      241 actcaatata cttatcaagt tcttctttag gtctattttc caataatagt tgtactaaag
      301 ttccataagc tactttttca ccatgtgtta aatgatgaat gtcacctgtc aatgcagtaa
      361 aaccattatg aattgcatgc gccgcagcta atcctccact ttcaaaacct agaccactca
      421 ataaagtatt agcttcaaca atattttcta atgctggtgt caccacttta gcttcacaag
      481 ctgccatagc ctgtaaacca tctgcaaaca gcgtttcttc acatttcttc gcaattgcaa
      541 ctccagccaa tgtttgctgt tgtcccaaca tagtttttcc atttgcctgc ataaccgcac
      601 gcgcctcaac ccaagttgct aaaccatctg caataccaga cgctaataaa cgcttagggg
      661 cttgtgaaat aacttttgta tcaaccaaaa ctaaatctgg atttttagaa taaaatagat
      721 aatgatcaaa tgcaccttca tctgtataaa taacagataa agcagataca ggtgcgtcgg
      781 tcgatgcaat tgttggagca ataataacag gcttttcaat caaatctgca atagcttttg
      841 cgctatcaat cgtctttccc ccaccaagac cgataatact atcacaattt tctttctcag
      901 ccaaggcaac aactcgattg atttcattgt cagaagcttc accattaaat agcgccagaa
      961 caatatggaa accataccta tgtagataat cttcaaatcg ttttccaaca atatcataaa
     1021 ccaactgatc gcataataga atagggcaat ttcccaaatc caaaattgat ttggcatttt
     1081 caaacaaggc attttccccc tgaatatatc tagaaggact agcaaaaatt ctcatatgta
     1141 tttcctctct atctaaatgg aacgactatt ttgaataaca aaccagtcgt cagaaaagtc
     1201 atcaaccgcc ttttggatag atggcatggc gaaagctgat tcaaaaacat ctgctcctgc
     1261 tgtaacagca tgcgcacctg cagctaaagc attatttact tgtgctacat ttttaaagga
     1321 tgcagctaaa atcttactag gagagttctg tctatcaata gcaagagcta attgacgaat
     1381 gacagaattt gaatcaatgt tcagattttc cattctatta taatatggag ctaggtaatc
     1441 cgctcctgct tcgatagcta ataatccctg aataactgta taaatagctg ttgcagtgat
     1501 atggtagccc tcttttttta gcacctttat tgcacgtaat ccagctggag taacaggtac
     1561 tttgataaat atatcatctc ctgcttgtct tcgaatttca tgagcatcct ttaagatgcc
     1621 ttcaaaatct tgagaaatca cctgaacatg aatagagggt gtagagccaa tcaattctct
     1681 tacatctttg attcgttcaa aaaaattaat agaaccctct ctttttgcaa tagtgggatt
     1741 tgaagttacc ccagctagcg gcaaaatttc agaccacttt ttaatctcat ctaaatttaa
     1801 tgtgtcaagc ataaattcca taaaaagaac ctctttattt tacaaagtat gctcagtacg
     1861 tcctataatg tcatcttggg ttgccttaga aagaacattg aagaatgcag agtatcctgc
     1921 aacacgaaca attaagtctc tgtgtttttc aggatgtttc tgagcgtcaa tcaacgtctc
     1981 tctggaaaca acattgtatt gaatatggta cccatgtaaa cgattaaaga atgttcgtaa
     2041 caaagcaatt agttttaatt tatcttcttc tttggctaac gtttgaggat ttactttctg
     2101 atttaagaga accccaccta cgatttcatc tgttggtaat tttgaaacag attttaaaac
     2161 agatgtaggg ccgtgttgat ccatattatg tgatggtgaa caaccctctg ctaacggtgt
     2221 acccgcgttg cgtccatctg gagttgctaa tgttccacgt ccctgcccta cgttggctga
     2281 tatagaagat gttcctgaat aacgaattcc tccaataggc cctcttccat aacgtgtatt
     2341 aggatattta gcaatttcat caacataaat gtcataagca gcagtaacca atttgtcagc
     2401 ataatcatca tcattaccat acttaggtgc atcatgaatc aacatttctt gaatgacctt
     2461 accttcttct ccggcataat ctgtttccag tgcatgccaa agctgacttg ggcttatacg
     2521 ttcttcctca aacaccaatt ttttaattgc agctaatgaa tccgacaaat ttgcaattcc
     2581 aacttgcaat cctgatatat aatcatatac tgctccacct tctttaaggt gttttccacg
     2641 accaatacaa tcatcagtca atgctgaaca tagaatatca ggaacttctc gttccaatga
     2701 taaatcaata gaattttcaa caataacact cattcgtgtc aaatatctta gtgttttatc
     2761 ccaagcattt tctaattcag aaaagttctt catatcctta aaatgaccaa agcttggtgc
     2821 aaaccgttta cccgaagccg gatcaattcc atcattcatc gtgataagta gaaccttagg
     2881 gaagttcata taactcatac ctgtgcaacg atagccccat ttccctggaa ctgccgtttc
     2941 aacacatcca atggcactgt aatcataagc atcatcttcc aatactcctt ttgcaataaa
     3001 agaaggaata ataatctcat cattattaaa tgcaggcata ccaaaaccaa gtttcatcac
     3061 ttcaatacac tcattcatga aacgagcatc taaacctgca tggtaacgta cagttagatt
     3121 aggttgcggt agatgggttt gtgcaactga ttttaatacc aaataagata atgggttaac
     3181 agcatccttc ttatctcgag tctgtccacc aattgtaaca ttttgatata aaggacttcc
     3241 tgctgaagaa aatgtatgtg attgactgcg aaccttatta attgtaattg tcttaatcca
     3301 aagatttgtc agacgttcaa caatgctatc ttctgtttct ttaccacttt ctaaatcagc
     3361 cttcatatat ggatacatat attgatcaaa acggccatat gaaagagagt ggccattaga
     3421 ttcaatttgt aaaatacatt gaataaacca aactgattga atagcttctg caaaagtagt
     3481 tgccggttca tatgggactc tagagcaaat atctgcaatc tcaagtaatt ctttcttacg
     3541 tttaggattt gcattttcgg ctaaactttt agcaagagca acaaagcgct ttgcatatac
     3601 tttaatagca tcgattacga taaatataga gtcgtaaaaa tgatatttat caatacttgc
     3661 tggatctgtt aaatctagag ctaccttcgc tttacgagcc cgctcttcaa aacctcttaa
     3721 accaaattgc aaaagtttct gatagttaac tgctaagtga gcatctccag aattcatctt
     3781 accttccata ccgaagaatc ctgtttccat ataaacagac acttcttcag gtaataaggc
     3841 accagctcta gcacgtaaat tattattttc ccaaaacgga gcaatacttc taagttgttc
     3901 ttttgtttct tctgtaatat agaaaacatc tccatcacgc ttttcaaaaa gatccaactc
     3961 attgagaaca aattctagcg tatattccgg aaaaatagga gcatctttat tggaagaagc
     4021 ttgatttccc gcaatcatag attcttcttc aatatagata gacatatttt ccaaaatttc
     4081 tttcagcata tatgcacgtt ttaggacatt aggttgttcc ttgtatcgtt cataggcgcg
     4141 tgttgctaga actgctctct cagcatcaat ataaggtttt ttatttaaaa catcttctcg
     4201 atatttgttc atcctttcag taaggctgcc aaaatattct gtcttgattg ttgttcttgc
     4261 tacttctgta tttaccatcg taagcacctt tctttcgaaa atatttttat ttctttcgaa
     4321 aatataatac cgatttttat agaaattttc aagcttttaa ctag
//
LOCUS       DAYYQN010000095         4296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYYQN010000095 DAYYQN010000000
VERSION     DAYYQN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.096"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(172..933)
                     /gene="pezT"
                     /locus_tag="ACHBRQ_001933"
     CDS             complement(172..933)
                     /gene="pezT"
                     /locus_tag="ACHBRQ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HFM9593535.1"
                     /translation="MGIQDYTDSEFKQALERNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(933..1409)
                     /gene="pezA"
                     /locus_tag="ACHBRQ_001934"
     CDS             complement(933..1409)
                     /gene="pezA"
                     /locus_tag="ACHBRQ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HFM9593536.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(1480..1752)
                     /locus_tag="ACHBRQ_001935"
     CDS             complement(1480..1752)
                     /locus_tag="ACHBRQ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593537.1"
                     /translation="MFTKLFKKNQDNSDIFKKLIHRLSDMSVQDLEKIDRLLDIIFTP
                     DQGSEQLKTESTYREETLDDTLKEAKNQLHKEQLEKNLERFRKNSQ"
     gene            complement(1790..2173)
                     /locus_tag="ACHBRQ_001936"
     CDS             complement(1790..2173)
                     /locus_tag="ACHBRQ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5945 family protein"
                     /protein_id="HFM9593538.1"
                     /translation="MTKDWNFNQPLESKSENQEDPDKIAALFGNHQGGNDVNYEAAFQ
                     KRKQAPVTESNSSSKPKVTEVRTGKETDITTSYQQHLKRLIADNNSDIQSSQKKIEEL
                     HTLIDTKNKDNKKLQSIYDAISELH"
     gene            complement(2170..2403)
                     /locus_tag="ACHBRQ_001937"
     CDS             complement(2170..2403)
                     /locus_tag="ACHBRQ_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5965 family protein"
                     /protein_id="HFM9593539.1"
                     /translation="MTIIERLEEKVTRQENKVARETEKLAAYKEQLETAMFATFKRRQ
                     SISHMSFEEALDHAFGRERQFDDSEFRKDEMSE"
     gene            complement(2454..3539)
                     /locus_tag="ACHBRQ_001938"
     CDS             complement(2454..3539)
                     /locus_tag="ACHBRQ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9593540.1"
                     /translation="MEIEECKQISILDVASRLGISFKQVSSSIYELPEHDSFRIFSNT
                     NTFKWFSRDIQGDVIDFVRLVKGISFKEALGFLSQEPFQKEAVQEKRERPFYYPLKRI
                     EDSNCSLARYYLTECRGISEEIIQKMIQQGLIAQASWKTNETVEPVIVFKSFNHRHKL
                     QAASLQGIYKNPALSRERLKTILKGSHGNVGISFDIGKPNRLVFCESFIDLMSYYELH
                     QQSLSDVRLVSMEGLKRSVVAYQTLRLIAEENQKLEFLDTVTPSKLLPLINTIRDTTT
                     YFNNHPDLLTLAVDCDDAGKDFSDKLSQEGFPVLLDLPNNEFGKEKIDWNDILREKKS
                     DLQLMIETAKETLRNQPVRQTSQCLEL"
     gene            complement(3549..4187)
                     /locus_tag="ACHBRQ_001939"
     CDS             complement(3549..4187)
                     /locus_tag="ACHBRQ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9593541.1"
                     /translation="MLNKVKTKALISVGAVATTSFILMMGYTVGQHSTAKQSRKEIEL
                     AAAKLVEDKQAEDKASILSSDTVKEFLTQYYTKEKLGENNTRIQPYMTESAYSQELTS
                     QNDAMNQVYKDYILDYHFEKADIFVNQTTNQAIAMVSYNVTYVSDLKNANQSKTNQTE
                     TRTVQLSYSKLPGKLLVNQVQVWKSGLDDLDKATTKNLEESSIPSLPNTTTK"
BASE COUNT         1233 a          823 c          637 g         1603 t
ORIGIN      
        1 tgcagtccaa ttagaaaata gcttgtaatc ataagaaaca ggtgtatgca aatgaattaa
       61 agtcttgtga aagctaccga atttctcttt ggaatttgtt agttcttgat aaacagactt
      121 tatcttgtta ttaaaccctc tcatttctat tctcctaaaa atatcaattg tttatttttc
      181 aagtaattca ttaagtctct tttcccccac ctgcaacatc tccttctcta cctgactcca
      241 ctccccaaag agtaactctt gaagaacatc tgctgctgaa gttgtatttt cttttgaatc
      301 atatacacaa cttctatctc gttggtaaat ttgaatgctt tcaaagatag ccagttcttc
      361 caattgtcgt gtattatcaa ctagatgatt tacaatgaaa tcatgatgtt cttttggagt
      421 tgcgcgtgct tgatttggat tgataatgta cagttcttca taacggataa gagtacttag
      481 atacgacaat tcaggctttg tcgcaattaa ggccaattgt acttcatatc ccttattttt
      541 caagagttgt gctgttttct ttggaacatc aactgttcgt aaagttccct cgatcaaaag
      601 attgtatctc aaactactca attttgttac taaagactct accatttttc ctgcaaaatc
      661 tttggtatat tctacactgt ctttgccata ttcttgctga agttctaaat agtgtggatg
      721 ctgagaacga aaactatcgc catctatgat aacaatattt ccttgaaact ctttctgttt
      781 aatacgatga attgtagtct ttccagcacc actttgccct ccaagcaaaa tcgctatagg
      841 ttgcttactg gacttttttc ctcttgtcag agagcgaagg tttcgctcta aagcctgttt
      901 gaattcacta tcggtataat cttggattcc cattaggcta ccatccgttt ttcagatatc
      961 tctaacatac gttcaattcc atccaaatag ccgctatacc tctctatttc atcaaaagtt
     1021 tctactaaat agatattcgt atgaatcaag tcagatagat catcactcat taaaatccaa
     1081 ggattagatt catcatcaat gctaattccc tgactatctt gataacgata gagtcgagat
     1141 agtagattag cacctctttc tttcacaatt tcaattttta aagtcaattc ataatcttca
     1201 acaggattaa gcattttatc ttctcctaca atatcgacat aagatacatt aaacttctga
     1261 caaatgatgt ctattagttc cgtagagact gaactcgttc cattttcata ccggctcaag
     1321 ctatttcgag aaattcctac aattcgggca aagtcgtctt gtgttaagtc atgtgtttta
     1381 cgtaaggatt ttatgttctt tccaatcatg gcaaactcct tttattttta taccaccatt
     1441 ataacattta gaaaaagtaa atgcaccatt tttggtgcat tattggctat ttttcctaaa
     1501 tctctctaaa ttcttttcca attgttcctt atgaagttga ttctttgctt cctttaatgt
     1561 gtcgtccaaa gtttcttctc tataagttga ttctgttttt agttgttctg atccttgatc
     1621 tggagtgaaa atgatatcta gcagtcggtc tattttttca agatcttgaa cggacatatc
     1681 agatagtcga tgaattagct tcttaaatat atcactattg tcttgattct tcttaaaaag
     1741 ttttgtaaac atctatagcc tctcaaaaaa agcagcctct taggactgct taatgtaatt
     1801 ctgaaatcgc atcataaatt gactgcaatt tcttattatc tttattcttt gtgtcaatta
     1861 acgtatgtag ctcttcaatt ttcttctgac tgctttgaat atcgctattg ttatccgcaa
     1921 tcagtctttt aagatgttgc tgataacttg tagtgatatc tgtttctttt cctgttctaa
     1981 cctctgtaac tttgggttta gaactagaat ttgattctgt cacaggtgct tgttttctct
     2041 tttgaaaagc tgcttcataa tttacatcat tacctccttg atgatttcca aataaggccg
     2101 caattttatc tggatcttct tgattttctg atttactttc taatggttga ttaaaattcc
     2161 aatcttttgt cattcactca tttcatcctt tctaaattca gaatcatcga attgtctttc
     2221 tctaccgaag gcatggtcaa gagcttcttc aaaactcatg tgactaatgc tttgacgcct
     2281 tttgaacgtc gcaaacatcg ctgtctccag ttgctctttg taagcagcaa gtttttctgt
     2341 ctctcttgct accttgtttt cttgcctagt gaccttttct tctaggcgtt caataatggt
     2401 catatacgaa cctccttcat tttaatatta gctaaaacat cttgattttg ttatcacaat
     2461 tctaaacatt gagaggtttg tcttactggt tgattcctca atgtctcttt tgcagtctcg
     2521 atcatcaatt gtaaatctga tttcttttct cgaagaatat cgttccagtc tattttttct
     2581 ttcccaaatt cattattagg taaatccagt aaaacaggaa atccttcttg agataactta
     2641 tcagaaaaat cttttcctgc atcatcacaa tctaccgcaa gtgtcaataa atcaggatga
     2701 ttattgaaat aggtagtggt atcacgaatt gtattgatca aaggcaataa ctttgaaggt
     2761 gttactgtat ctaagaattc caacttctga ttttcttcag ctatcagtcg taaagtttga
     2821 taagcaacaa cagacctttt taatccctcc atagatacca agcgaacatc tgatagactt
     2881 tgttgatgaa gttcgtaata gctcatcaag tcgataaacg attcacaaaa gaccaatcta
     2941 tttggtttac caatgtcaaa ggatattcca acatttccat ggcttccttt tagaatcgtt
     3001 tttaacctct ctctagaaag agcgggattc ttataaatcc cttgtaagct tgctgcctgc
     3061 agcttgtgac gatgattaaa gcttttaaaa acgataacag gttcaactgt ttcatttgtt
     3121 ttccagctgg cttgtgctat taaaccttgt tgaatcatct tttgtatgat ttcttctgag
     3181 attcctctac attctgttaa gtaatatctg gccagactac agtttgaatc ttctattctc
     3241 tttaaaggat aataaaatgg tctctctctc ttttcttgaa cagcttcttt ttgaaaaggt
     3301 tcttgagaaa gaaaacctaa agcttcttta aaagagattc ccttaacaag tcgaacaaaa
     3361 tcaatgacat caccttgaat atctcttgaa aaccatttaa aagtattggt attcgaaaaa
     3421 atccgaaatg aatcgtgttc aggaagctca tatatactgc tagaaacttg tttaaaggag
     3481 atacctaaac gacttgctac atcaagaatt gaaatttgct tacattcttc tatttccatg
     3541 caatatcatc attttgtcgt agtatttggc agtgatggta ttgatgattc ctctaaattt
     3601 ttagtagttg ccttatctaa atcatctaac ccagatttcc aaacctgcac ttggttgacc
     3661 aataacttac caggaagttt agaataagat aattgaactg ttctggtttc tgtctgattg
     3721 gtctttgatt ggttggcatt ctttaaatca gatacatagg ttacattata agacaccatg
     3781 gcaatggctt gattcgtagt ctgattgaca aaaatatcag ctttttcaaa atgataatcc
     3841 aaaatatagt ccttataaac ttggttcata gcatcatttt gacttgtcaa ttcttgagaa
     3901 taagcagatt cagtcatata gggctgaatg cgtgtattat tttctccaag cttttctttc
     3961 gtataatact gtgtcaaaaa ttcttttaca gtatctgatg acaaaatgct tgctttatct
     4021 tctgcttgtt tgtcctctac aagtttagct gcagccaatt cgatttcttt gcgactttgt
     4081 ttagcagtag aatgttgccc aacagtatat cccatcatga gaataaagct tgttgtggct
     4141 actgctccaa cactaattaa ggctttagtt ttgactttat ttaacatctt agaccttctt
     4201 tctataaaaa ctaggtcaag aataacaaaa agaacttgta agttttatca cataatgagt
     4261 taaacatatc tattaaaatt ttaacacctg tatcct
//
LOCUS       DAYYQN010000096         4242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYYQN010000096 DAYYQN010000000
VERSION     DAYYQN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.097"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..385
                     /locus_tag="ACHBRQ_001940"
     CDS             <1..385
                     /locus_tag="ACHBRQ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9593542.1"
                     /translation="KSHGDILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLDSI
                     FSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLR
                     MIISALQNTIPFESIKERIRPHYGS"
     gene            375..695
                     /locus_tag="ACHBRQ_001941"
     CDS             375..695
                     /locus_tag="ACHBRQ_001941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9593543.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            758..2533
                     /locus_tag="ACHBRQ_001942"
     CDS             758..2533
                     /locus_tag="ACHBRQ_001942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9593544.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTISIGQKVSTGDILGTVKETEVVNHKIMVPYGISGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQALGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDDKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSENERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIIEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIQLILETGGL"
     gene            2534..3919
                     /locus_tag="ACHBRQ_001943"
     CDS             2534..3919
                     /locus_tag="ACHBRQ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9593545.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVKFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3942..>4242
                     /locus_tag="ACHBRQ_001944"
     CDS             3942..>4242
                     /locus_tag="ACHBRQ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9593546.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKAFAVAKSLKNSLMVEELFSIPSKEIELFVEKENIMS"
BASE COUNT         1383 a          630 c          906 g         1323 t
ORIGIN      
        1 taagagtcat ggggatattt tacaattact ttcagatgaa ggaagtattt ctgctaaaga
       61 atttatatac attgtagaaa atcaagaaat atttgtgtgg ttcaataaaa taaatccaag
      121 cttagattca atcttttcaa cttatgaatt gaagatgcag gacgcaacaa tttcatcttc
      181 tgagttagaa tttttatgtg atttactatt gtataaaact ttagatcaag gaaggtacaa
      241 tgtagagggg ccgttagttc ttgctagata tttattggga tgtgagtttg aagtaaagaa
      301 tctcagaatg atcatatcag ctcttcaaaa tacaattccc tttgaatcaa taaaagaaag
      361 gatacgccca cattatggaa gctaataagt ataaaattgg cataattggt agccgtgata
      421 ttattttacc atttagcatg attgggtttg atatatttcc tgcctaccaa gaacaagaag
      481 ctataaatac actaagaaaa ttagctcaat ctgattatgg tgtcatttat atcactgaag
      541 acattgcttc aatgatatta gatacaattc gccattatga ttcccaagtt gtgcctgcta
      601 ttattttatt accgactcat aaacaaggtt taaatttagg attaaaacgt atagaggata
      661 atgtagagaa agcagtagga cacaatattt tataataatg tacaaaattg tctgtaatat
      721 tattctataa tttttggact tagtaaggag aataactttg actcaaggga agattataaa
      781 agtatcggga cctctagtta ttgcatcagg tatgcaggag gctaatattc aagatatttg
      841 ccgtgtaggt aagctagggt taatcggtga aattattgaa atgagaagag atcaggcatc
      901 tatccaagtc tatgaagaaa catctggtct tggtccggga gaacctgttg ttacaactgg
      961 agaacctctc tcggttgaat tagggcctgg attgatttct caaatgtttg atggcataca
     1021 acgcccatta gatcgattta aattggctac tcataatgat tttctagttc gtggggtaga
     1081 agttccaagt ttggatagag atattaagtg gcattttgat tccactatat caattggtca
     1141 aaaagtgagt acgggtgata ttcttggaac tgtcaaggaa accgaagtag ttaatcataa
     1201 aattatggtt ccttatggaa tatctggcga agtcgtttct attgcctctg gcgattttac
     1261 aattgatgaa gttgtatatg aaataaaaaa attggacggt agtttctata aaggaacact
     1321 tatgcaaaaa tggcctgtcc gcaaggcgcg tcctgtttct aaacgtttaa tcccagaaga
     1381 accattaatc acaggtcaac gagttattga tacattcttt ccagtaacca aagggggagc
     1441 agcagctgtt cctggaccgt ttggagcagg aaagacagtt gtacaacacc aagtagctaa
     1501 atttgccaat gttgatattg ttatttatgt cggttgtgga gaacgtggaa atgaaatgac
     1561 ggatgtactg aatgagtttc ctgagttgat tgaccctaat acaggacaat caattatgca
     1621 acggacagtt ctgattgcta atacttcaaa tatgcctgtt gctgctcgtg aggcttcaat
     1681 ttatacagga attaccatgg ctgagtattt tcgtgatatg ggctactctg tcgccattat
     1741 ggctgattcg acttcacgtt gggcagaagc gctacgtgaa atgtcaggac gtctagaaga
     1801 aatgcctggt gatgagggtt atcctgctta tcttggaagt cgtatcgctg aatattatga
     1861 aagagcggga cgttctcagg ctctagggct tccagaacgt gaaggaacga ttactgctat
     1921 tggagctgta tcgccacccg gtggagatat ttcagaacca gttactcaaa acactttacg
     1981 gattgtgaaa gttttttggg ggcttgatgc tccgttggca cagcgacgtc attttcctgc
     2041 aattaactgg cttacctctt attcactcta taaagacagt gtgggaactt atatagatga
     2101 taaagagaag acagattgga atagtaaaat aactcgtgcg atgaactact tacaacggga
     2161 atctagttta gaggaaattg ttcgtcttgt tggaattgat tctctgtctg aaaatgaacg
     2221 actaacgatg gaaattgcta aacaaattag agaagattat ttgcaacaga acgcttttga
     2281 ttcggtagat acattcactt cgtttgcaaa acaagaagca atgttaagta atatcctaac
     2341 ttttgctgat caggcaaatc atgctttaga gttgggttct tactttacag agattataga
     2401 aggtaccgtg gcagttcgag accgtatggc gagaagtaaa tatgtttcag aagatagatt
     2461 agatgaaatc aaaattattt caaatgagat tacacatcaa attcaattga tattagaaac
     2521 aggaggtcta taaatgagtg ttataaaaga atatagaact gctagtgaag ttgttgggcc
     2581 tcttatgatt gttgaacaag ttaataatgt gtcttacaat gagttagttg aaattcaact
     2641 tcataatgga gaaattcgtc gtggacaagt tttagagatc cacgaagata aagcaatggt
     2701 tcagcttttt gaaggatcta gtggaataaa tttagaaaag tctaaaattc gttttgctgg
     2761 tcatgcacta gaattggctg tatctgagga tatggttggt agaattttta atgggatggg
     2821 aaaaccaatt gatggtggac cagatttaat tccagagaaa tatttagata ttgatggtca
     2881 agctattaat cctgtatcta gagattatcc agatgaattt attcagacag ggatctcctc
     2941 tattgatcat ttgaatactc ttgtacgtgg tcaaaaatta ccagtatttt caggttcggg
     3001 cttacctcat aatgaattag cagctcagat agcaagacaa gcgactgttt taaattctga
     3061 tgaaaatttt gcggttgtat ttgcagcaat gggtattact tttgaagaag ctgagttctt
     3121 tatggaagaa ctcagaaaaa caggagcgat cgatcgttca gtcttattta tgaacttggc
     3181 aaatgatcct gcaattgagc gtattgcaac tccccgcatt gctttaactg cggcagagta
     3241 tctagctttt gaaaaagata tgcatgttct agttatcatg acggatatga ctaactattg
     3301 tgaagcgtta cgtgaagtct cggcagctcg ccgtgaagtt ccaggtagac gaggctatcc
     3361 gggatattta tatacaaatt tatcaactct atacgaaagg gctggtcgct tagttggtaa
     3421 aaaaggttca gtgacacaga ttcctatttt aacaatgcca gaagatgaca taacacatcc
     3481 aattcctgat ttaactggat acattactga agggcaaatt attttgtcgc atgagttgta
     3541 taatcaaggt tatcgtccac caatcaatgt tttaccttct ctctctcgat taaaagataa
     3601 gggatctgga gaaggtaaaa ctcgtggaga tcatgctcca actatgaatc aactgtttgc
     3661 agcctatgcc caagggaaaa aggttgaaga gttagcagta gtattaggag aatcggcttt
     3721 atctgatgta gataaattgt atgtgaagtt tacaaagcgt tttgaagaag agtacatcaa
     3781 ccaaggattt tataaaaatc gaaatattga agatacgttg aatcttgggt gggaattact
     3841 atcaattctt cctagaacag agttaaaacg tatcaaagat gatttgcttg ataaatactt
     3901 acctttggta gaagtttaat ccagaaatgg agtgattatc tatggtgcgt ttgaatgtaa
     3961 aaccaactcg tatggaattg aataacttaa aggaacgttt gaaaacagct gaacgtggac
     4021 ataagttatt aaaggataaa agagatgaat tgatgaggcg atttatttct ttgattcgtg
     4081 agaataatca acttcggaaa gaagtagaaa gttatctaat tgataatcta aaagcctttg
     4141 cagttgctaa atcattaaag aactctctaa tggtggagga attattttca attccatcga
     4201 aagaaattga attatttgtt gagaaagaaa atatcatgag tg
//
LOCUS       DAYYQN010000097         4229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYYQN010000097 DAYYQN010000000
VERSION     DAYYQN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.098"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..1078
                     /gene="dnaN"
                     /locus_tag="ACHBRQ_001945"
     CDS             <1..1078
                     /gene="dnaN"
                     /locus_tag="ACHBRQ_001945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9593547.1"
                     /translation="AISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNED
                     AGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQ
                     YPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVAT
                     DSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENI
                     SFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKD
                     GVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAV
                     RPFTLVPADTDEDFMQLITPVRTN"
     gene            1143..1337
                     /locus_tag="ACHBRQ_001946"
     CDS             1143..1337
                     /locus_tag="ACHBRQ_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HFM9593548.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANCWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1421..2536
                     /gene="ychF"
                     /locus_tag="ACHBRQ_001947"
     CDS             1421..2536
                     /gene="ychF"
                     /locus_tag="ACHBRQ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9593549.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2608..3177
                     /gene="pth"
                     /locus_tag="ACHBRQ_001948"
     CDS             2608..3177
                     /gene="pth"
                     /locus_tag="ACHBRQ_001948"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9593550.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3178..>4229
                     /locus_tag="ACHBRQ_001949"
     CDS             3178..>4229
                     /locus_tag="ACHBRQ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9593551.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVT"
BASE COUNT         1451 a          660 c          844 g         1274 t
ORIGIN      
        1 agctattagt tctaaaaatg ccattcctat tttatcaaca gtaaaaattg acgtgaccaa
       61 tgaaggtatt actttaattg gttcaaatgg tcaaatttca attgaaaatt ttatttctca
      121 aaaaaatgaa gatgctggtt tgttaattac ttctttaggt tcgatccttc ttgaagcttc
      181 tttctttatc aatgtagtat ctagtttacc tgatgtaact cttgatttta aagaaattga
      241 acaaaatcaa attgttttaa ccagtggcaa atcagaaatt accctaaaag gaaaagatag
      301 cgaacaatat ccacgaatcc aagaaatttc agcaagcact cctttaatac ttgaaacaaa
      361 attactcaag aaaattatta atgaaacagc ctttgctgca agtacacaag agagtcgtcc
      421 gattttaaca ggtgtccact tcgtattgag tcaacacaaa gagttaaaaa cagttgcaac
      481 agactctcat cgcctaagcc agaaaaaatt gactcttgaa aaaaatagtg atgattttga
      541 tgtcgtaatt cctagccgtt ctctacgcga attttcagcg gtatttacag atgatatcga
      601 aactgtagag attttctttg ccaataacca aatcctcttt agaagcgaaa atattagctt
      661 ctatactcgt ctcctagaag gaaactatcc tgatacagat cgcttgattc caacagactt
      721 taacactact attactttta atgtggtaaa cttacgccag tcaatggagc gtgcccgtct
      781 tttatcaagt gcgactcaaa atggtactgt gaaacttgaa attaaggatg gggttgttag
      841 cgcccatgtt cactctccag aagttggtaa agtaaacgaa gaaatcgata ctgatcaggt
      901 tactggtgaa gatttgacca ttagtttcaa cccaacttac ttgattgatt ctcttaaagc
      961 tttaaatagc gaaaaggtga ccattagctt tatctcagct gttcgtccat ttactcttgt
     1021 gccagcagat actgacgaag acttcatgca gctcattaca ccagttcgta caaattaagt
     1081 gaaagaggtt gagcctggct cgcctctttt atgatataat cgaaaaagaa aaggagagta
     1141 gtatgtatca agttggaaat tttgttgaga tgaaaaaatc acacgcttgt acaatcaagt
     1201 cgactggtaa aaaggctaat tgttgggaaa ttacacgtgt aggagcagat atcaaaataa
     1261 aatgcagtaa ttgtgagcat gttgtcatga tggggcgata tgattttgag cgaaaaatga
     1321 ataaaattat tgactgagaa cccttagtta gagggttagc actttatccc tttttgtgtt
     1381 ataatattag ggattgaaat gaaaacggag aatgagaaat atggctttga cagcaggtat
     1441 cgttggtttg ccaaacgttg gtaaatcaac actatttaat gcaattacaa aagcaggagc
     1501 agaggcagca aactacccat ttgcgacgat tgatccaaat gttggaatgg tggaagttcc
     1561 agatgaacgc ctacaaaaac taactgaaat gataactcct aaaaaaacag ttcccacaac
     1621 atttgaattt acggatattg cagggattgt aaaaggagct tcaaaaggag aagggctagg
     1681 gaataaattc ttggccaata ttcgtgaagt agatgcgatt gttcacgtag ttcgtgcttt
     1741 tgatgatgaa aatgtgatgc gcgagcaagg acgtgaagac gcctttgtag atccacttgc
     1801 agatattgat acaattaatc tggaattaat tcttgctgac ttagaatcag tgaacaaacg
     1861 atatgcgcgt gtagaaaaga tggcacgtac gcaaaaagat aaagaatcag tagcagaatt
     1921 caatgttctt caaaagatta aaccagtcct agaagacggg aaatcagctc gtaccattga
     1981 atttacagat gaggaacaaa aggttgtcaa aggtcttttc cttttgacga ctaaaccagt
     2041 tctttatgta gctaatgtgg acgaggatgt ggtttcagaa cctgactcta tcgactatgt
     2101 caaacaaatt cgtgaatttg cagcgacaga aaatgctgaa gtagtcgtta tttctgcgcg
     2161 tgctgaggaa gaaatttctg aattggatga tgaagataaa aaagagtttc ttgaagccat
     2221 tggtttgaca gaatcaggtg tagataagtt gacgcgtgca gcttaccact tgcttggatt
     2281 gggaacttac ttcacagctg gtgaaaaaga agttcgcgct tggactttca aacgtggtat
     2341 gaaggctcct caagcagctg gtattatcca ctcagacttt gaaaaaggct ttattcgtgc
     2401 agtaaccatg tcatatgaag atctagtgaa atacggatct gaaaaggccg taaaagaagc
     2461 tggacgcttg cgtgaagaag gaaaagaata tatcgttcaa gatggcgata tcatggaatt
     2521 ccgctttaat gtctaaaaat taataaatgg tgtcaattag gttggaaaaa aaattccaac
     2581 ccttttggct tttgaaagga aaaataaatg accaaattac ttgtaggctt gggaaatcca
     2641 ggggataaat attttgaaac aaaacacaat gttggtttta tgttgattga tcaactagcg
     2701 aagaaacaga atgtcacttt tacacacgat aagatatttc aagctgacct agcatccttt
     2761 ttcctaaatg gagaaaaaat ttatctggtt aaaccaacga cctttatgaa tgaaagtgga
     2821 aaagcagttc atgctttatt aacttactat ggtttggata ttgacgattt acttatcatt
     2881 tacgatgatc ttgacatgga agttgggaaa attcgtttaa gagcaaaagg ctcagcaggt
     2941 ggtcataatg gtatcaagtc tattattcaa catataggaa ctcaggtctt taaccgtgtt
     3001 aagattggaa ttggaagacc taaaaatggt atgtcagttg ttcatcatgt tttgagtaag
     3061 tttgacaggg atgattatat cggtatttta cagtctattg acaaagttga cgattctgta
     3121 aactactatt tacaagagaa aaattttgag aaaacaatgc agaggtataa cggataaatg
     3181 gtgaccttat tagatttatt ctcagaaaat gatcagatta aaaaatggca tcaaaattta
     3241 acagataaga aaagacaact aatacttggt ttatcaacat ctactaaggc tcttgcaatt
     3301 gcaagcagtt tagaaaaaga agataggatt gtgttattga cgtcaactta tggagaagca
     3361 gaaggacttg ttagtgatct tatatctatc ttgggtgagg aactcgtcta tccatttttg
     3421 gtagatgatg ctcctatggt ggagtttttg atgtcttcac aggaaaaaat tatttcacgg
     3481 gttgaagcct tgcgtttttt gactgattca tctaagaaag ggattttagt ttgtaatatc
     3541 gtagcaagtc gattgatttt accgtctccc aatgcattca aagatagtat tgtaaaaatc
     3601 tcagttggtg aagaatatga tcaacacgcg tttatccatc agttaaagga aaatggctat
     3661 cgaaaagtta ctcaagtaca aactcagggc gaatttagtc ttcgaggaga tattttagat
     3721 atttttgaaa tatcccagtt agaaccttgt cgaattgagt tttttggtga tgaaattgat
     3781 ggtatcaggt catttgaagt agaaacacaa ttatcgaaag aaaataagac agaactcact
     3841 atctttccag ctagtgatat gcttttgaga gaaaaggatt atcaacgagg acagtcagct
     3901 ttagaaaaac aaatttcaaa aactttatca cctattttga aatcatacct agaagaaatt
     3961 ctttcaagtt ttcaccaaaa acaaagtcat gcagactctc ggaagttttt atctttgtgc
     4021 tatgataaga catggactgt ctttgattat attgaaaaag ataccccaat attctttgat
     4081 gattatcaaa aattgatgaa tcagtatgaa gtctttgaaa gagacttagc gcagtacttt
     4141 acagaagaat tacagaatag taaagcattt tctgatatgc agtatttttc tgatattgaa
     4201 caaatctata aaaaacaaag tccagtgac
//
LOCUS       DAYYQN010000098         3757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYYQN010000098 DAYYQN010000000
VERSION     DAYYQN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.099"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..380
                     /locus_tag="ACHBRQ_001950"
     CDS             <1..380
                     /locus_tag="ACHBRQ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecF"
                     /protein_id="HFM9593552.1"
                     /translation="LGQKVVAMLDFLLAYSDYSKDFRPLIIDQPEDNLDNRYICRHLV
                     QQFRDVKAQRQIILATHNATIVTNSMTDQVVIMESDGVNGWIESQGYVSEKYIKNHII
                     NQLEGGKDSFKHKMSIYETALSE"
     gene            1199..1558
                     /locus_tag="ACHBRQ_001951"
     CDS             1199..1558
                     /locus_tag="ACHBRQ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HFM9593553.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1568..1933
                     /gene="mobC"
                     /locus_tag="ACHBRQ_001952"
     CDS             1568..1933
                     /gene="mobC"
                     /locus_tag="ACHBRQ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9593554.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            1920..>3757
                     /locus_tag="ACHBRQ_001953"
     CDS             1920..>3757
                     /locus_tag="ACHBRQ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
                     /protein_id="HFM9593555.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKTYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEEIARRLDLFVKIINTNKFTVL"
BASE COUNT         1351 a          576 c          759 g         1071 t
ORIGIN      
        1 gtttgggaca aaaagttgta gccatgcttg attttttatt agcatatagt gattattcta
       61 aagacttcag accattgatt attgatcagc ctgaagacaa tctagacaat cgttatattt
      121 gcaggcattt agttcagcag tttagagatg tgaaagctca acgtcaaatt attttagcaa
      181 cacataatgc tacaattgta acaaattcta tgacagatca agttgttatt atggagtcag
      241 atggagttaa cggatggatt gaatcacagg gatatgttag tgaaaaatat ataaaaaatc
      301 atatcatcaa tcaattagag ggaggaaaag attccttcaa gcataaaatg tctatatatg
      361 agacggcttt atcagagtag agtcagaaaa agtaggttag aaatttagcc tacttttttc
      421 tttgtccgac aggcatagtg tgcatctgag gtccaagtcc tctgtggata tttgctgcag
      481 atgaaaccaa tagcgactcc taagcctgaa tatcgtgagg taggggggat aggaaggaat
      541 tagcgaaatc aaggttctac aaacagaatc gtgacttgaa gccatatata gcggatgagg
      601 aactctaaaa tccaaatagg tgtcgtaacc tatatacgta aattacgaga gtaaactagg
      661 aaagatgtac ggcttattcc gtgagcgttt aggacgtagt acaacgaatc atgggagtca
      721 gctgaacaca tagtattgaa gaaatttctg taatggaaat ggagcgaaga agtgaacaat
      781 taaatgaata cctctctaat taaatttgtc aattctaatt cctggtatga aaagacagtg
      841 acctgaaaat gtaaacgatg ggagctgatt ataaatatag gacggtacat gcagtggtgt
      901 tagagattag tccctacttg atttgtgata acttccccaa atttcttctg ctatactttt
      961 ctcaactttt aaaaatccaa ctaagaattt tacctggggg tttgggggcg aagccaccaa
     1021 gttatcttat cgttggctgt caaaactgga aggttttggt tagctggcga tatgattttt
     1081 tgggtattgt aaccacaatg cttgagctcg caaagaccga acaaggactt taagaaattt
     1141 cccgtatgac acacggggtc ggggtttacc cgagaaagga tgaaagtgag gaaaagagat
     1201 gtctgaacag taccgtgaca ttcgaaaaga ggtaaatcta accgcagatg agctaaaaca
     1261 gattgaaaaa atgatggagg ttgataatta tcgtcacttt tcaccattcg tcagggataa
     1321 aattttgatg actgatgaca aacagttagc tgccaaagaa tggttttccc tttggcagtc
     1381 tcaaaagttt gaacagatca gtcgagatgt gcatttggtt ttgattatcg caagagagaa
     1441 tcaccaagtg actcaagaac acgtgtcaat cttattgacc tgcgttcaag aattgattgc
     1501 ggaagtcaat caagtacagt cactcagtcg tgggtttcgt gaaaaataca tgagatagga
     1561 ggctactatg gtttatcgtt atcgtaccaa tctcaaaaaa gtatttctaa cagattcaga
     1621 attacaccaa ttgaatgaac ggattgctaa gagcaattgt caaaatttct cagtctatgc
     1681 cagaaaagtt ttgcttaatc ccaatatgtc atttgtcacg attaacactg atacttatga
     1741 acagttagtg tttgaattga ggaggattgg aaataacatc aatcaaattg cgcgtgcgat
     1801 taatcaaagc catctgattt ctcagaacca attacaagaa ttgagtaaag gagtcggaga
     1861 gttaattaag gaagtggaca aagaatttca agtggaggtg aaaagactga aggagtttca
     1921 tggtagttac taaacacttt gcgacgcacg gaaaaaaata tcgtaggcgt ttaattaagt
     1981 atattctcaa ccctgataaa acggacaatt tgaaattggt atctgatttt ggcatgagca
     2041 attacttgga cttccctagt catgcagaaa tggtagagat gtacaatgtc aacttcagca
     2101 ataatgacaa gttgtacgaa tccagaaacg accgacaaga aaaacaccaa caaactattc
     2161 atgctcatta cctcattcaa tcattttctc ctgaagataa tctgacacct gaagaaatta
     2221 accgcattgg ttatgagatc atgatggaat tgacaggagg tcgttttaag tttatcgtag
     2281 caactcacac agacaaagat catgttcata atcacatcct aatcaacgct attgaccgca
     2341 attcagataa aaagttgata tggaactatg ccttggaacg aaacctccgt atgatttcag
     2401 accgtatttc taaagtgact ggtgcaaaaa ttattgagaa acgttattcc tatcgagatt
     2461 ataaaaaata tagggaatct agtcataagt ttgaattgaa acaacgtctt tattttttgc
     2521 tacagcaatc taaatcgttt gatgattttt tagagaaagc aaagcagttg catgttcaga
     2581 ttgattttag tcaaaagcat agccgttttt tgatgacaga tagaacgatg ataaaaccaa
     2641 ttcgaggacg ccaactcagc aaacgagatt tatatgatga agactttttt aaaacatatt
     2701 ttgccaaaca agagattgaa agtcgtttaa aatttttact aaagtctgtt tattcattag
     2761 aagaattgca tgtaaaggct aaagagttga atctaaccat cgagttaaaa cagaaaaatg
     2821 tgatgtttac cctagaagaa gatggcaaaa agataagcct gagtcataaa aaaattagcg
     2881 ataagaaatt gtacgatgtt caatttttta atcgttattt tgaagataga gaagttagag
     2941 atattcaagc attagaaaat cttcaggaag attttcagac ttttcgagag gagcagcata
     3001 aggagaaagt ctccgctgaa gaaatcgagg aagccttcaa gaaatacaag gaaaaacgag
     3061 atgctatcca tgagtttgag attgaactta cggataatca gatagagaag ttggttgatg
     3121 atggtgtcta tatacaggtt tcgtttggta tcaaacagag tggactcatc tttattccta
     3181 actaccagct agatattttt gaggaagaca atcataaaaa atataaagtc tacattcgag
     3241 aaacgtcttc ttactttgtc tataataaag aaaacatgga taataattgt ttcatcaagg
     3301 gtaggacctt aatcagacaa ttgagcaacg atagccaaaa actcccctat agacgaccaa
     3361 ctttaaaaag tcttaaagag aagatttctg aaattaatct tatgattgaa ctatctaaca
     3421 cgaacaaaca ataccaagaa atcaaagatg agcttgtatt agaaattgca gagattgata
     3481 tgcagctaga agaaacccaa gaaaaaatcg ccaccttaaa caagatggcg gaagtcctta
     3541 tcaatttgaa gagtgaagat catgagacta gaaaattagc taagtatgat tttgaccaaa
     3601 tgaacatgac ggaatcaata atgttagatc gactcaatac ggatatttta aaattacaac
     3661 aggaacttgg taatgaaatt aataaatatg aggagatagc tagaagattg gatttgtttg
     3721 tgaaaattat taataccaat aaatttacag ttctaaa
//
LOCUS       DAYYQN010000099         3735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYYQN010000099 DAYYQN010000000
VERSION     DAYYQN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.100"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            67..1248
                     /locus_tag="ACHBRQ_001954"
     CDS             67..1248
                     /locus_tag="ACHBRQ_001954"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HFM9593556.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1306..2064
                     /locus_tag="ACHBRQ_001955"
     CDS             1306..2064
                     /locus_tag="ACHBRQ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9593557.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            2277..3638
                     /gene="dnaA"
                     /locus_tag="ACHBRQ_001956"
     CDS             2277..3638
                     /gene="dnaA"
                     /locus_tag="ACHBRQ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9593558.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1328 a          647 c          669 g         1091 t
ORIGIN      
        1 tgtgtttaag ttataattaa gctagtcagt ttatactttc agtaattcaa acatatggag
       61 gcaaatatga aacatctaaa aacattttac aaaaaatggt ttcaattatt agtcgttatc
      121 gtcattagct tttttagtgg agccttgggt agtttttcaa taactcaact aactcaaaaa
      181 agtagtgtaa acaactctaa caacaatagt actattacac aaactgccta taagaacgaa
      241 aattcaacaa cacaggctgt taacaaagta aaagatgctg ttgtttctgt tattacttat
      301 tcggcaaaca gacaaaatag cgtatttggc aatgatgata ctgacacaga ttctcagcga
      361 atctctagtg aaggatctgg agttatttat aaaaagaatg ataaagaagc ttacatcgtc
      421 accaacaatc acgttataaa tggcgccagc aaagtagata ttcgattgtc agatgggact
      481 aaagtacctg gagaaattgt cggagctgac actttctctg atattgctgt cgtaaaaatc
      541 tcttcagaaa aagtgacaac agtagctgag tttggtgatt ctagtaagtt aactgtagga
      601 gaaactgcta ttgccatcgg tagcccgtta ggttctgaat atgcaaatac tgtcactcaa
      661 ggtatcgtat ccagtctcaa tagaaatgta tccttaaaat cggaagatgg acaagctatt
      721 tctacaaaag ccatccaaac tgatactgct attaacccag gtaactctgg cggcccactg
      781 atcaatattc aagggcaggt tatcggaatt acctcaagta aaattgctac aaatggagga
      841 acatctgtag aaggtcttgg tttcgcaatt cctgcaaatg atgctatcaa tattattgaa
      901 cagttagaaa aaaacggaaa agtgacgcgt ccagctttgg gaatccagat ggttaattta
      961 tctaatgtga gtacaagcga catcagaaga ctcaatattc caagtaatgt tacatctggt
     1021 gtagttgttc gttcggtaca aagtaatatg cctgccaatg gtcaccttga aaaatacgat
     1081 gtaattacaa aagtagatga caaagagatt gcttcatcaa cagacttaca aagtgctctt
     1141 tacaaccatt ctatcggaga caccattaag ataacctact atcgtaacgg gaaagaagaa
     1201 actacctcta tcaaacttaa caagagttca ggtgatttag aatcttaatt gacatctatg
     1261 taaagaaagc tttacataag agaaaagatg tgttagtgta gaatcatgga aaaatttgaa
     1321 atgatttcta tcacagatat acaaaaaaat ccctatcaac cccgaaaaga atttgataga
     1381 gaaaaactag atgaactagc acagtctatc aaagaaaatg gggtcattca accgattatt
     1441 gttcgtcaat ctcctgttat tggttatgaa atccttgcag gagagagacg ctatcgggct
     1501 tcacttttag ctggtctacg gtctatccca gctgttgtta aacagatttc agaccaagag
     1561 atgatggtcc agtccattat tgaaaattta cagagagaaa atttaaaccc aatagaagaa
     1621 gcacgcgcct atgaatctct cgtagagaaa ggattcaccc atgctgaaat tgcagataag
     1681 atgggcaagt ctcgtccata tatcagcaac tccattcgtt tactttcctt gccagaacag
     1741 attctttcag aagtagaaaa tggcaaacta tcacaagccc atgcgcgttc cctagttggg
     1801 ttaaataagg aacaacaaga ctatttcttt caacggatta tagaagaaga tatttctgta
     1861 aggaaattag aagctcttct gacagagaaa aaacaaaaga aacagcaaaa aactaatcat
     1921 ttcatacaaa atgaagaaaa acagttaaga aaactactcg gattagatgt agaaattaaa
     1981 ctatctaaaa aagacagtgg aaaaatcatt atttcttttt caaatcaaga agaatatagt
     2041 agaattatca acagcctgaa ataaggctgt tcttttattt ttttatctca caaggttatc
     2101 cactatgttt ttcgataaaa agcttaataa atcaataatt tcttctttta tccccaacct
     2161 gtggataaag tttggtaaca ttgtggatta tttttcacag cttgtggaaa attcttgcta
     2221 tctatggtaa aatatctcta gtattaaact tttaaatagt aaaggaggag aaaggattga
     2281 aagaaaaaca attttggaat cgtatattag aatttgcaca agaaagactg actcgatcca
     2341 tgtatgattt ctatgctatt caagctgaac tcatcaaggt agaggaaaat gttgccacta
     2401 tatttctacc tcgctctgaa atggaaatgg tctgggaaaa acaactaaaa gatattattg
     2461 tagtagctgg ttttgaaatt tatgacgctg aaataactcc ccactatatt ttcaccaaac
     2521 ctcaagatac gactagctca caagttgaag aagctacaaa tttaactctt tataactata
     2581 gtccaaagtt agtatctatt ccttattcag atacgggatt aaaagaaaag tatacctttg
     2641 ataactttat tcaaggggat ggaaatgttt gggctgtgtc agccgcttta gctgtctctg
     2701 aagatttggc tctgacctat aaccctcttt ttatctatgg aggaccaggc cttggtaaga
     2761 ctcacttatt aaacgctatt ggaaatgaaa ttctaaaaaa tattcctaat gcgcgtgtta
     2821 aatatatccc tgccgaaagc tttattaatg actttcttga tcacctaaga cttggggaaa
     2881 tggaaaagtt taaaaagacc tatcgtagtc ttgatctttt gttaatcgat gatatccagt
     2941 cactcagcgg aaaaaaagtc gcaactcagg aagaattttt caataccttt aacgcccttc
     3001 atgacaagca aaaacagatt gtcctaacga gtgatcgtag tccaaaacat ctagaagggc
     3061 tcgaggagag gcttgtcacg cgttttagtt ggggattgac acaaactatc acaccccctg
     3121 actttgaaac acgtattgcc attttacaaa gtaaaacgga acatttaggc tacaatttcc
     3181 aaagtgatac tctagaatac ctagctgggc aatttgattc aaatgttcga gatcttgagg
     3241 gagccatcaa cgacatcact ttaattgcca gagtaaaaaa aatcaaggat atcactattg
     3301 atattgctgc agaagccatt agagcccgca aacaagatgt tagccaaatg ctcgtcatcc
     3361 caattgataa aatccaaact gaagttggta acttttatgg tgttagtatc aaagaaatga
     3421 agggaagtag acgccttcaa aatattgttt tggcccgtca agtagccatg tatttatcta
     3481 gagaactaac agataatagt cttccaaaaa ttgggaagga atttggggga aaagatcata
     3541 ccacagtcat tcatgcccat gccaaaataa aatctttgat tgatcaagac gataatttac
     3601 gtttagaaat tgaatcaatc aaaaagaaaa tcaaataatt tgtggataac ttttagtttt
     3661 ttatcttttt tatccacatt ttttaaacaa gctaaaaaac ttgatatgac ttgtttaaag
     3721 gctgttttcc acaga
//
LOCUS       DAYYQN010000100         3730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYYQN010000100 DAYYQN010000000
VERSION     DAYYQN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.101"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(155..3067)
                     /gene="nanA"
                     /locus_tag="ACHBRQ_001957"
     CDS             complement(155..3067)
                     /gene="nanA"
                     /locus_tag="ACHBRQ_001957"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HFM9593559.1"
                     /translation="MNRSIQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKENEYFTMSVLDNTALIEGRGANGEQFYDKYTDAPLKVRPGQWNSVTFTVEQPTAE
                     LPHGRVRLYVNGVLSRTSLKSGNFIKDMPDVNQAQLGATKRGNKTVWGSNLQVRNLTV
                     YDRALSPEEVQTRSQLFERGDLEKKLPEGAKISEKEDVFEGGMNNKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKERTIREEF"
     gene            complement(3355..3582)
                     /locus_tag="ACHBRQ_001958"
     CDS             complement(3355..3582)
                     /locus_tag="ACHBRQ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593560.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
BASE COUNT          984 a          852 c          628 g         1266 t
ORIGIN      
        1 cacatcttat ttcaatctac tatattttat agaacagact actctgaaag tagtttcaga
       61 cctcttatga tttcgtatca gcctgaatgt catcaaaaaa agatagcagg cttgaaaacc
      121 tgctatctcc ttctattttt acaaaatcaa atgtttagaa ttcttctctt attgttctct
      181 cttttttccc tagcgtaaac agaccaagga agaaagctgt tagtcctagt gaagctagga
      241 ggtcactctc cttgttccct gtttcaggaa gtgcctgctg agcaagtgga gctttgtaag
      301 tataatcttc ttttgtagta agagttacaa gcgaatcaga tcccttatac tctgcgattt
      361 catgaacagc tggctctgta ccattaactc cccctgtaaa ttctggaact tcatgcaccg
      421 ctgctttgct accatttact ccgccaggaa ctctggcacc tgctagattt acaggaagat
      481 tatgcatact ttcgatgctt cctttagcta taccaataat ttcctgtccg atatcttcct
      541 tatctactgc aaacaacaag gttttgctat catactgggt taggaaagtc gctgttttac
      601 catttgccaa ttgaagagtt ggagccttgt tgaccaatac ttctgagtcg aactccaagc
      661 caataactcc tttgcccatc tctcctttgc ccatctctct agttcgcttc actttcgctt
      721 cggtaggaga aatcagattt ttgctcaaaa attcccaatt aaattttcta aatgataggg
      781 tataggcatt ttgtcctttt tcagtatgtt catacaagat gccatactcc ccatttccta
      841 attcttggag cgaattatag gcaaactctc ctttttgaat tggattgtgt ttgagccaag
      901 tcaactcacc attttcttcg acacgtgcca agtggaccat cccattttca cgtttcggtc
      961 cacctgcatt actgaggatg atgtattctt ttccttcgtg catcgtatgg atagcagaca
     1021 tttgaacata gacatcttta acctgtggat aacgtttgat atccttctcc caagtcactc
     1081 ctccgtcttt acttgtagca acctgaagat ctccagtcaa accacgcata aagagtttaa
     1141 catctccatt gtttagttgt accaccgttg attctgtatt ttgcgcacgt ttattgttca
     1201 tcgtagaaga gtggatcttt tgaccgtcta cctgacggtt atcgttgacc gcttctccag
     1261 catgccaagt ttttccatga tcatctgaat agatgacacg agaagattgc gagccattta
     1321 agtgagatac attattagtc gtataaaccg gtatcaaaat ccgtcccttg tgaggcccat
     1381 tccgaagtac aattcctgtt ccaggaccta cacccaagaa tttcatccaa tcggctttga
     1441 ccatcggagt aatatcttga ggagctgacc atgtcttccc gtcgtcatca ctgtaggaca
     1501 tccatagata gctatccttg gcaattctaa atggggaagt tttgtttgtt gtgaagtaga
     1561 tattacctag taattggtca cccttgtata gatcaccctt gtcgctatag gctggtttaa
     1621 caggatctac aacaacgcga tagtctgtcg ccttgccatc tggtgtgtag acagtaccat
     1681 tttcacgaat ggtataagct cccttttctc cttcacgata gaggatttga taggtttttc
     1741 cgtcaatttc tttgtaggct tcttcttttt gcgaagacat tccaaagatt cccttccctt
     1801 ctgggaacat gtcatagatg gaaaagattc gtttggtttc aggatcttga accaacacca
     1861 tatcgatatt cactggtgaa ccgatcgatg gatcagaagc ttttggattg tcacgtaagt
     1921 tggtaatggt tactcggtct ccccaagttt taccattgtc ttcactacgt ctgataacca
     1981 taccgatatc accccagtca ctagaatgaa gacggcgttc atctgcacct gcaatcagag
     2041 ttcctttatc tgtcttgaga agagctggaa tacgataact cttaattcca tctttatttg
     2101 gtttgttgtt cattccccct tcaaagacgt cttctttctc tgagattttc gcaccttctg
     2161 gaagtttctt ctccaaatca cctctttcaa atagttggct tctcgtctga acttcttctg
     2221 ggcttaaggc acgatcatag acggttaggt ttcgaacttg tagattgctt ccccaaactg
     2281 tcttattgcc ccgtttggtt gctccaagtt gagcttgatt aacatctggc atatctttga
     2341 taaagttacc agatttgaga cttgttcgag ataagacacc gttcacatag agacgaactc
     2401 gtccatgagg taactccgct gtcggttgtt cgacagtaaa ggtcacggaa ttccattgtc
     2461 ctgggcgaac tttcaaagga gcatctgtat acttatcata gaactgctct ccgttagcgc
     2521 cacgcccttc aataagagct gtattatcaa ggaccgacat agtaaagtac tcattttcct
     2581 tggtgtcact ggatacagag aagagattgt aaaagcgtgg agctgatgca tctggcttaa
     2641 actccatgtg aactgttgcg ttttcaagtt tctttagttt atctagttca cttgataaat
     2701 caactctctg accatttgaa gcattggttt caacatcttc tttttctatc actgtttcga
     2761 cattttccaa atctcgtgca tagtaatctc ttggaatttt ttcttcttgc ttatctttcc
     2821 tttcttgttc ttgcttattg ccagaaagtt cagtctctga agaaggctgg ctcttttctg
     2881 tatcagttag ggttgagctc ccccccgaaa gttgagtttc atttgccaga ggttgctcac
     2941 ttgccccttc ttgagctaaa acaggagacg ttccaaatac cactgctcct acaatcatag
     3001 aaaccgctcc taccgatagt ttcctaatgc tataacgaca cttacgttct tgaatactcc
     3061 gattcatact gatcctctct atatctatat cccgatttct gaaataagac atttactcca
     3121 gaaaatgcac ttagtttgtc ttttattaac tttcttttac tacagttcat ttaaatgaca
     3181 aaaatgaact aaacaaacta tctcaaattg gtgccaattt tcccctctcc tttctccaat
     3241 cagactcctg ccaatctagt ttatctacca gggcaccgct gcaattaaac taaaatatct
     3301 ctttataagc aaatgatgga catcagctta tgctatttat accatacttc ctgctcaatc
     3361 cttatcgaac actttttcaa atagcttttc ataagcataa atacttgtag aaataatggg
     3421 aaatataatc aagctcacaa tcagaatctt atccagctta aaggggacaa tcccaaataa
     3481 gtgaagaaat aaatcaatca gaaaactgtc gaaaaaaact gtcaaaagta gttcaacttg
     3541 tctttttctc tgcttttttg tcttgaatgc cttaaatatc atattattcc ccttttctaa
     3601 gcttcaatcc cttctctcta tctgttattt cgcatagcta ctactatttt tacagcaatc
     3661 tatctttaaa tatacataaa gatagattct atcacttttt ataagcgtta gtaaatttat
     3721 tatacctcat
//
LOCUS       DAYYQN010000101         3674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYYQN010000101 DAYYQN010000000
VERSION     DAYYQN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.102"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            211..909
                     /locus_tag="ACHBRQ_001959"
     CDS             211..909
                     /locus_tag="ACHBRQ_001959"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9593561.1"
                     /translation="MNYIDTNEMLFVETPRKVITSDELRKKNTKYLDQKEFKLFIQNL
                     KDEALCDYRITKYIRIAKVLFLTGMRYGELAALNYKEDIDFSKKTIHIKHTYDFRQKE
                     RTTPKTIKSDRVITAPQKVLDIIKEQIIENATNGFDTDFIFINTLGEPITNARVICAL
                     KRHGQKIGIEKNITTHTFRHSHISLLAELGIPLTAIMDRVGHSDSKTTLEIYSHVTQK
                     MVSDISSKLDKIKF"
     gene            complement(992..1085)
                     /gene="ffs"
                     /locus_tag="ACHBRQ_001960"
     ncRNA           complement(992..1085)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBRQ_001960"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1118..1585)
                     /gene="tadA"
                     /locus_tag="ACHBRQ_001961"
     CDS             complement(1118..1585)
                     /gene="tadA"
                     /locus_tag="ACHBRQ_001961"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9593562.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(1786..3072)
                     /locus_tag="ACHBRQ_001962"
     CDS             complement(1786..3072)
                     /locus_tag="ACHBRQ_001962"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9593563.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(3303..3539)
                     /gene="comW"
                     /locus_tag="ACHBRQ_001963"
     CDS             complement(3303..3539)
                     /gene="comW"
                     /locus_tag="ACHBRQ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HFM9593564.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         1298 a          816 c          530 g         1030 t
ORIGIN      
        1 gaaacacggc gtaaaaaact caaccgtcta cgcttcaaag aatgtaaaaa aagagatttt
       61 aaagcagata gagggcgact acctagttag aaatttgata gacgtttatt acaaaaagta
      121 atagatcaac tattacaaga tgggagatct cataactatg tttctaaaat caaattcaag
      181 ctcaatcaga taatgaaatt tgctatcaga atgaattata ttgatacaaa tgaaatgcta
      241 tttgttgaaa cgcctagaaa agtaattaca tccgacgaac tcagaaagaa aaatacaaaa
      301 tacttagacc aaaaagagtt taagttattc atccaaaatt taaaagacga ggccctatgt
      361 gattatcgaa ttacaaagta tatccgaata gctaaagttc tttttcttac tggcatgagg
      421 tatggagagc tggcagcctt aaactacaag gaagatatag atttttctaa aaagactatt
      481 cacatcaagc atacatacga tttcagacaa aaagagagaa ctacaccaaa gacaatcaag
      541 tccgataggg ttataacagc acctcaaaaa gtgttagata ttatcaaaga gcaaataata
      601 gagaatgcga caaatggatt tgatacagat tttattttca tcaatactct aggagaacca
      661 ataacaaatg ccagggttat ttgtgcattg aaaagacatg gtcaaaaaat cggcatagaa
      721 aaaaacataa ctacacatac atttagacat tctcacatat ccctacttgc tgagctgggc
      781 attcccttga ctgccatcat ggacagagta gggcatagtg actcaaagac cacactagag
      841 atttattctc acgttactca aaaaatggta tcagacatat ctagcaagtt agacaagata
      901 aaattttaaa ttgtgcccct cgtctgcccc tttttcttat acaagacaaa caaaaacccc
      961 ttaaagtgtt gattttaaag ggtttttaaa gtgcacgaaa aaagagcaca caattcaaat
     1021 cgcttagggc tgctggattc ctcccctgac ccgcttcacg cagaactgtt gctccactat
     1081 ttattatatc acattcctat tcattttaaa agcaaaatta ttttttccgt ctatttctaa
     1141 aaaaatcctg catgatagct gcgcattcat cttccaaaat tcctatttca acctccacac
     1201 gatgattgag acgctcatct gtcaagatat cgtacaaact cccagcagcg ccaaatttct
     1261 ggtttttagc cccatagacc acatttggaa tgcgggcaag cccaatcgcc ccactacaca
     1321 tgacacaagg ttcaatggtc acaaaaagtg tgcaatccag caagcgccag ctctcctcac
     1381 tcaagttcgc atcctctata gccataattt ccgcatgcat aaccgctcgc tgtaattcct
     1441 cacgcgcatt atgcccacga ccaatgattt ccccatcttt gacaatcaca caaccaattg
     1501 gaatttcatc gtgttcaaga gcaatctcag cctctctcaa agcctccctc ataaagactt
     1561 ctttttcttc aagcgtataa tacatctctt ttctcttttc ctacttatcg attttattat
     1621 tatatcatga atcccaacac aaaagaagcc accgaatgcg gtgactctat agggagatta
     1681 ttatgaaaaa gaaaagttta ggatatttgt tacaacaagt taggaggtct tcttgtaact
     1741 gtctatagta tacccgacct atcttaaaca aatcttaaaa atctcttagg accaaacact
     1801 ttctaaaata tttgtttgtt cacgaccagg acctactgag aaagtagaaa tacgaacgcc
     1861 aaccaattca ctcacacgac gaacatagtt acgcgcattc tcaggaagat cttccaaatt
     1921 gcgaactccg gtaatatctt ctgaccaacc tggcaactct tcatagatag gcttgcaacg
     1981 tttcaattgc tcaagactag ctggatagta gtcaatacgt tgaccgtcaa gatcataggc
     2041 cacacagatt ttcacagtat ccaaaccgct caaaacatca atagagttca aagaaaggtt
     2101 agtaatacca gaaacacgac ggctatgacg catcacaact gagtcaaacc aacctacacg
     2161 acgtggacga ccagttgttg taccatactc atgacccact tcacggatac gttctcccac
     2221 ttcatcaaac aactcagttg ggaaaggacc atctcctaca cgactcgtat aagctttaca
     2281 tacacctaca accttgtcaa tcttgcttgg accgacacca gaaccaattg tcacaccacc
     2341 agctacaggg tttgatgacg taacaaatgg ataagtacct tggtcgatat ctagcataac
     2401 accttgtgca ccttcaaaaa gcacacgttt gccattatca agcgcatcat tcaagataac
     2461 agatgtatct atcacgtatt tcttgatttg ttgaccatat tcgtaatatt cttcaaaaat
     2521 atcatcgaaa acaatcgctt tactgtcata caatttttca aaaagacgat tcttttcagc
     2581 aaggttacgt tctaaacgct cacggaaaat atctttatct aaaagatctg caatacgaat
     2641 tccaacacga gcagccttgt ccatataagc tggaccaatt cccttaattg tcgtaccaat
     2701 cttattgtcg cccttagctt cttcttgcaa gcgatccaac tcgatatgat aaggcaaaat
     2761 aacatgcgca cgatcagaaa tacgcaagtt atcagttgtt acaccttcct catgaagata
     2821 gctcaactct tttacaagag atttaggatt tacaaccata ccattcccaa tgacagatat
     2881 tttttcaggg aagaaaatcc cagatggaat caagtgcaac ttaaatttct taccgtcaat
     2941 cacaatcgtg tgaccagcat tatcaccacc ttggtaacgt gcaatcactt ctgcattcgc
     3001 tgaaaggaag tctgtaatct tccctttacc ttcatcaccc cattgggtac ctacaacaac
     3061 aactgaagtc ataattttgt ctgagccctc aggctcttcc tttctcacat acatggcagg
     3121 actctcacct gcaattatat cttacaattt attataataa aaaatcgcct ttttatcaag
     3181 aagaaacaat agaaagattt gctatttcca actattaaaa aatgatttag aaaaattact
     3241 agctatttac tattatcttt acataaaaga gtaaattagt tcggaaattt actaaaatta
     3301 cctcaacaag aaataaaacc ccgattcatt accaattttt caagatacaa acgataagca
     3361 acacgataat ggtaaacgat aaaatcccta tgacaaccaa tgccatatct cactaaataa
     3421 taaattaaaa atttaaaatg aacatgttcc cagtcaaaat tatcaccaaa tgtaggacca
     3481 tactcttctt caatactatc atagaaatta gccatctgct cataaatttt ttgtaacata
     3541 atcaaatact ccttttcttt tttataaact tattctaaca aaaaatttta cacattcact
     3601 atcaattcct gaattgttaa aataccagcc tcaacaagat aaaaatagga aaagttgaca
     3661 aaatagaaac aaat
//
LOCUS       DAYYQN010000102         3410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYYQN010000102 DAYYQN010000000
VERSION     DAYYQN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.103"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            48..242
                     /locus_tag="ACHBRQ_001964"
     CDS             48..242
                     /locus_tag="ACHBRQ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9593565.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(364..530)
                     /locus_tag="ACHBRQ_001965"
                     /pseudo
     CDS             complement(364..530)
                     /locus_tag="ACHBRQ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(608..1918)
                     /locus_tag="ACHBRQ_001966"
     CDS             complement(608..1918)
                     /locus_tag="ACHBRQ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFM9593566.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2155..3027
                     /gene="nadC"
                     /locus_tag="ACHBRQ_001967"
     CDS             2155..3027
                     /gene="nadC"
                     /locus_tag="ACHBRQ_001967"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFM9593567.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3158..>3410
                     /locus_tag="ACHBRQ_001968"
     CDS             3158..>3410
                     /locus_tag="ACHBRQ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFM9593568.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKPRKVDRDRLKNYLTDNPDAYLTEIASEF"
BASE COUNT         1074 a          565 c          757 g         1014 t
ORIGIN      
        1 catttaaaaa aattttattt ttttattttt cattacgaat aatatagatg aaggggaaag
       61 agtatgaaaa cagaactgtt tcttttgcta ttagttcaaa aggagaaaaa atgaaagtag
      121 aaaatatttc gtatagggtg gatcatcgta tattgtttga taatatttct tttgatactt
      181 cgagttcagg cgtgacatta attactggta aaaatggtac aggaaagtca actttactat
      241 agtagattga aactagaata gtacacatct acttctaaaa tattgttaga aatcgatttg
      301 actatcctga tctatttgtc ctgttcttat ttcatttcac tatatctcaa attgagtatg
      361 acgaagtgcg ctcccatgtc ctgggaacgc actttcttca tatttttcat attcttgaat
      421 ccatcgataa agactattgg gatgaatttt taaagttgaa ctaatcattt ttacaggatg
      481 agatttacag cagagagttt gaaggcttta tcaaaggttt ttcttggcat aatgactttt
      541 cctcgtttcc acttaatttt gtgtctactt tattatacca agtccaccct taagttagat
      601 aataaatcta acttaaggaa gctagaagga tgagaatcca ggtggtcaag agtcccaaac
      661 ttaagctgat ggggacaccc agaataattt gctttttgaa ggcaaggcca cgttcctcta
      721 tattgggaag tgagagttga atgagagaac cagctgatga aaagggtgag atattagtag
      781 atagagcgcc aataacggtg gctgttgtga gtaagtgaat atcaatctga ggattttgag
      841 cactgatgat agcaatgatg ggaaagaggg ctggagctac aacggatagg gtggaactaa
      901 agagtgacat cactccggct atcacacaaa agaacagagg taaccagaaa tgaggaatgg
      961 ttgttgtcat gaggtgccct atcagtgtga ctaaacctga cttgaccgct agagacatta
     1021 gtaagctcat gccgcagagc atgataattg tagcccaggg aaccttagct aaaatggctt
     1081 cttgcttccc taatttgagc cttaaggcga ggcagaccat gagtattgag acaaagccaa
     1141 tatcaaatgt tttttgataa gtagctatcc aggcgatgtt tgggaaaatg agatgcaaca
     1201 agggaaaaag ccaaaccaaa accatgctgc tgatcatgag caaggtggtt tgtctttgaa
     1261 ccttgctgag gagtggtggt tggtcaatag tcaaggatga gtttgttctt cccttactat
     1321 agtgactgta acaggataat aaaagcaaga cgatgagtgg gtagataatg ctgacgataa
     1381 agatatgatt gccaagtgaa aaagcttgct cttcccatcc catttgctta aacaggcctt
     1441 gaaagacaat gcctgagcta ctggttatca aattagcccc tcctgaagct ccccaattga
     1501 cggcttgagc tccaatcaaa gggtgtttgt ccgctttttg acagagggta atcgctagag
     1561 gacagcaaac ggccatagta gtgaaaaatc cagcacctaa agcagacaaa agggttgcca
     1621 tcaggtataa aatcatgtag agggcgttag ggtgggtgcg tgtgcggtag agaatgtgtt
     1681 gagccaaaac atcaagagta ccgttagttg ttgcaacgtt ataaaagaga gagacgctaa
     1741 aaatggtaaa aaagagtgag gttggccaaa aatgaagaag ttctttgggg cttaatccca
     1801 tgagagtggt tgcgatgagg taagaaaaag caatagccag caggccaata ttgattttgg
     1861 tgcggtaacc aattccaatg gctagagcaa tggcgctaat cattattaaa tgaatcattg
     1921 aattgtcctt tcagttagaa tatggaaata ggttggcaat tttggcgctc aaaagaccac
     1981 ctgtttccta ttggtacttg actattatac acaaaaaaaa tacaattgtc ttgacaattg
     2041 tattgacact tgttagaatt aaaaatgagg tattcaggct tgtgatttcc tatggaagtt
     2101 aatagtaatt gcctctagtg cttacaagtg atattaaaaa tagaggactt agtgatgtca
     2161 atcatttcaa ctgatttaac cccttttcaa atagatgata cattgaaagc agccttgcga
     2221 gaagatgttc attccgaaga ttacagtacc aatgccattt ttgatcatca tggccaagcc
     2281 aaggtgtcgc tttttgccaa ggaagctggt gttttagcgg gactaaccgt ttttcaaagg
     2341 gtttttaccc tatttgatgc cgaggtgacc ttccagaatc ctcatcaatt taaggatggg
     2401 gatcgtttga ctagtggcga tttggtttta gaaatcatag gctcggtgag aagtctctta
     2461 acatgtgaac gcgttgcctt gaatttttta caacatttat cagggattgc ttcgatgaca
     2521 gctgcttatg tagaagcctt aggcgatgat tgcattaagg tatttgatac tcgaaaaact
     2581 actcctaatt tacgtctttt tgagaaatat gccgtgagag ttggcggtgg ctataatcat
     2641 cgctttaatt tatcagatgc tatcatgcta aaagacaatc acattgcggc agtaggtagt
     2701 gttcagaagg caattgctca agcgcgtgcc tatgcccctt ttgtgaaaat ggtcgaggtg
     2761 gaagtggaaa gccttgctgc tgccgaagaa gctgcggcgg cgggtgctga tattatcatg
     2821 ttggataata tgtcattgga acagattgaa caggccatta ccctaattgc aggacgttct
     2881 cggattgaat gttctggaaa tattgatatg accactatta gccgttttcg tggtttagcg
     2941 attgattacg tctccagtgg tagtttaacc catagtgcta agagtcttga tttttccatg
     3001 aagggtttaa cctaccttga tgtctaagtt gtaaaataaa ctaacttttt aaaggatgtc
     3061 tttcctctag aacgagtttt atgtcagata gtttaaacgc ctcttcaaat atagtaaaat
     3121 gaaccaaaaa tagtacacaa tgtggtataa tcttcttatg gcatattcaa tagattttcg
     3181 taaaaaagtt ctctcttatt gtgagcgaac aggtagtata acagaagcat cacacgtttt
     3241 ccaaatctca cgtaatacca tttatggctg gttaaagcta aaagagaaaa caggagagct
     3301 aaaccaccaa gtaaaaggaa caaaaccaag aaaggttgat agagatagac ttaaaaacta
     3361 tcttactgac aatccagacg cttatttgac tgaaatagct tctgaatttg
//
LOCUS       DAYYQN010000103         3332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYYQN010000103 DAYYQN010000000
VERSION     DAYYQN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.104"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..923)
                     /locus_tag="ACHBRQ_001969"
     CDS             complement(<1..923)
                     /locus_tag="ACHBRQ_001969"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9593569.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGREIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYG
                     SGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLF
                     YFCQLYNFEKLNKAVTIPSLTKSDLLNISIPL"
     gene            complement(958..1923)
                     /gene="xerA"
                     /locus_tag="ACHBRQ_001970"
     CDS             complement(958..1923)
                     /gene="xerA"
                     /locus_tag="ACHBRQ_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HFM9593570.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(2041..2454)
                     /locus_tag="ACHBRQ_001971"
     CDS             complement(2041..2454)
                     /locus_tag="ACHBRQ_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9593571.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(2451..2687)
                     /locus_tag="ACHBRQ_001972"
     CDS             complement(2451..2687)
                     /locus_tag="ACHBRQ_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9593572.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(2779..>3332)
                     /locus_tag="ACHBRQ_001973"
     CDS             complement(2779..>3332)
                     /locus_tag="ACHBRQ_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9593573.1"
                     /translation="NKNILLDLQLELLGIEEQENIICILNTIKRLITKRKLQLDELNL
                     LVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKNVTKNG
                     FSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGMVI
                     LRPKTPNLNHNWQSKNLWKNLKL"
BASE COUNT         1000 a          644 c          446 g         1242 t
ORIGIN      
        1 agggggatgg aaatatttaa taaatctgat ttagttaaac taggtattgt tacagcctta
       61 tttaacttct caaaattgta taattgacaa aaatagaata aatattctga gtttattttt
      121 tctaaaacag gctctaatcc aaatgctgta tctacattcc aaaaattttc tctaactaaa
      181 ataggtttat ttatatttcc tttacgtcca ataataactg aatttttctt cactatccaa
      241 tctttagcat atcccataat tcccccactt ccataaatag gaaatttccc atcagcatct
      301 tcaacctgtt tttgattttt accatttctt attgttagaa tctcgttcca tttgctaact
      361 ttccattctt tttcatttag aataacatcc ccaaacatct cgttaaatcg ggattttaca
      421 agtagattaa gctcttcaag ttgttcttgt ctccttaaaa tcagcttgct taacaaatcc
      481 agctcacttg caattctttg ttgctctccc aaatttgtat atggaactat aatattatca
      541 aaatattttt tagttaaatg tttcattgtg gaaccatgag tatgttttac agcatcttgt
      601 aaaccttttt caacaacata tttaaaatac gatttatcta tatctatctt atcaaaaacc
      661 actttaaaaa tgtgctgatt taatactgcg gatcttccac accactgaaa aacccctaat
      721 gtacccgacc acgaaataag gatatcacca gcttcaacaa tgtatttttt atcaatcgta
      781 cccgaataat aatttatctc tgttgaagtt ttagtcagat tctgtattct gataatctct
      841 ctcccttcag atgaccaatc ttgaggttta aaagcatagc cattgataaa agtagccact
      901 tcccctaatt tcactttctt catgcaacca cctcctcgga atatcgggat tttgctttta
      961 agagatgaat ttttggtgtg aatgcttgac attattttga ttgaccatgg cataggccag
     1021 ggttgtgtca atcttgctat gacctagcag tttttggact tgttcgatag gcataccttt
     1081 atcaattgcc ttagtcgcta aagttcttct gaacttatgt gggtgaacct tttgtatgcc
     1141 gagtttgtct cctaactttc ttaagcgaat ctctacacca gcaattccaa gtctctgggc
     1201 ttttccaact agcgttacaa aaagagcagg gtgactatct tttctgtcgt taagataatt
     1261 tcttaaatga attttcgtcc gagcatcaaa atatactggt ctctccttct ttcctttacc
     1321 aaagacaaca cactctctgt tttcaaaatc aatatctgaa cgattcaact gtacaagctc
     1381 ccctacacgc atacctgtcg atgctagtag gtctattatt gccaaatctc tcaaattttc
     1441 acagttatca cgcataattt ccaaatgttc atcagtataa gtttccttca cattttgctc
     1501 agtcttaatt ttctttatcc gtcgaatggg atttttaatg atatattcct cttgctcaag
     1561 ccaagcaaaa aaagaagaca atatacgcct aatattgtct aaatttgcct tactacaatc
     1621 cttttcagac tggtaatttg ctaaataact ccttaaatca tctgttgtga gttgtgtcac
     1681 agactcttta atagcattaa acaagttttc aatcgtcctc tggtaataac gaattgtttt
     1741 tgagctacag ccctctacct gcttggcggt aataaatagt tgcaacaggt tattattcga
     1801 atacttctgc tgcttttttc cgtctactag atgatgttct aaactctctc tcaacgctag
     1861 taattgttca ttatttaaaa gaggtaacat ttcttgcatg ataattgtaa cttttgattc
     1921 cataatttct cactttctta ttcatcaata aaaagagaca atatcagttt ctgcattaca
     1981 gaattctaat attgtctgtt catttatttt cttgtgctca ttcaaatcca gcatactagg
     2041 ttactttttg accttttctc taacagaatg ttcagaaacc catttggagt agcttgtcat
     2101 tttttcatca gttaaagctt ctactgcgat ggttacacac atttttactg cttcttctac
     2161 cgttacagaa aaacgatagc cgtttaattg taaaaatttg accaaaacga agaaagcagt
     2221 tcttttatta gcattagcaa aaacatgctt ctttatcaat tggacaaata gtatcgttgc
     2281 tttatcaaaa attgttggat aaagagactt cccaaagaca aattgttctg gtaagttcac
     2341 aatcatattt aaggcagaag aactaactgt ttgaattttc tcatttggag aataccgttg
     2401 aattgctaaa gcatttattt tttcaatttg cttttctgtc agatagattg tcatttttcc
     2461 accaaagctt tgaaaacatc gtcgtattcg tcaaatattt tatctaaatt taagtcaaag
     2521 gcatcatcag atacataaga aacctgctgc cccaattctt caggagtaaa gataatctca
     2581 ccacttggta acaatttcgc ttcgtattta acaccacttg gaatattaaa ttcacttgga
     2641 atcgtaatgg tgattgaatt tccttgtttt cttgttttta ctaccatctg atttcctcct
     2701 ttgaagtatt ataacaaatc attaccgtaa ttacaatggc agaatatcag ccaaaatact
     2761 cctgcatcag agatttcttc aaagtttcaa gttcttccag agatttttgg attgccaatt
     2821 gtgatttaga tttggtgtct tgggacgtaa tattaccata cctgaattta tacgtaaatg
     2881 tttatatttt attaattcat cgtagtacgc tacatttcca acagtacctc ttgttgtcaa
     2941 gactatatca taacgctcaa gtttgccttt tcgaagtaat ttatcctttg ttttagtgat
     3001 aaattgcttt gtatcgaatg aaaatccgtt tttagtaaca ttctttgtat ttaaaaataa
     3061 acagtactcc tcactaaaca actcatctga tttaggataa tttttgcccc tatcaccatc
     3121 tataatatca aataagttat caatgctttc aaatatttta ttttccccaa acatctcgtt
     3181 aaatcgggat ttgacgagca agtttagttc atctaactgc aattttcttt tagtaataag
     3241 ccttttaatc gtattaagaa tacagataat gttctcttgt tcttcgatac ctagcaattc
     3301 taattgtaaa tcaagtaata tattcttgtt ta
//
LOCUS       DAYYQN010000104         3210 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYYQN010000104 DAYYQN010000000
VERSION     DAYYQN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.105"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..1288)
                     /locus_tag="ACHBRQ_001974"
     CDS             complement(join(<1..847,847..1288))
                     /locus_tag="ACHBRQ_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSpn4 family transposase"
                     /protein_id="HFM9593574.1"
                     /translation="MKLSYEDKVQIYELRKQGYSLEKISNKFEINNSNLRYMIKLIDR
                     YGIEFIKKGKNRYYSPELKQEMIDKVLHENWSQDRVSLKYALPNRGMLPNWLAQYKKN
                     GYTIVEKTRERVPKMGRKRKKTWEEMTELERLQEENERLRTEVAYFKKVKRAGRQGRS
                     PRARKAEIVREMASGGFQLDLLLETARLARSTYYYQLKGLGGLDKDKDLKAEIQAIFT
                     EHKGNYGYRRVTLELRNRGFVVNHKKVQRLMKVLGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPASSQKLYLSPVLDGFNSEIIAYNLSTSPNLEQ
                     GKAMLNQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKTFQSLNQLEQAIVDYIDYYNN"
     gene            1873..2091
                     /locus_tag="ACHBRQ_001975"
                     /pseudo
     CDS             1873..2091
                     /locus_tag="ACHBRQ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2391..2678)
                     /locus_tag="ACHBRQ_001976"
                     /pseudo
     CDS             complement(2391..2678)
                     /locus_tag="ACHBRQ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2677..2940
                     /locus_tag="ACHBRQ_001977"
                     /pseudo
     CDS             2677..2940
                     /locus_tag="ACHBRQ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2963..3210
                     /locus_tag="ACHBRQ_001978"
                     /pseudo
     CDS             2963..3210
                     /locus_tag="ACHBRQ_001978"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT         1017 a          571 c          563 g         1059 t
ORIGIN      
        1 ttgttgttgt aataatcaat atagtctaca atagcttgtt ccaattgatt aagcgactga
       61 aatgtcttct cataaccata aaacatttcg gatttcaaaa tgccaaagaa ggactccatc
      121 atgccattgt ctgggctgtt tcccttgcgt gacatggatg cttgaattcc cttactctct
      181 aggaaccgat gataagaatc gtgttggtat tgccagcctt gatcgctatg gagaatggta
      241 ttctcataat gtttctctgt aaaggcctgg tttaacatag ctttcccttg ttctaagttt
      301 ggtgaagttg aaagattata ggcgataatt tcgctgttaa agccatctaa aactggcgat
      361 aaatagagct tttgactgct tgctggaatg gcaaactctg tcacatccgt atagcacttt
      421 tccattggtc tcgatgcttc gaactggcgt tgaatgagat tctctgcttt cttgccaatc
      481 tctccttggt aggaggaata ctttcgtttc cgacgaattc gagctgttaa accaagaact
      541 ttcatcagac gttgaacttt cttatgattc accacaaaac cacgatttct tagttcaaga
      601 gtaactctac gatagccata atttcctttg tgctcagtaa aaatagcttg aatttcagct
      661 ttaagatctt tatctttgtc aagcccacct agtcccttca actgatagta gtaagtcgag
      721 cgagctaaac gtgctgtttc aagaagtaaa tctaattgaa atcctcctga agccatttct
      781 ctaactatct ctgcctttct cgctctaggg cttcgtccct gtcttccagc tcttttaact
      841 tttttaagta agccacctcg gtacgtaagc gttcattctc ctcttgaagt cgctctagtt
      901 ctgtcatttc ttcccaagtt ttcttacgtt tacgtcccat tttaggtact ctctctcttg
      961 ttttctcaac aatagtatac ccgtttttct tgtattgcgc cagccaattt ggaagcatac
     1021 cacgatttgg gagagcatat ttgagagaaa ctctatcttg agaccagttt tcatgtaaaa
     1081 ctttatcaat catttcttgt tttagttctg gagaatagta tcgatttttc ccttttttga
     1141 tgaactctat tccgtaacga tcaatcaatt taatcatgta cctaagatta gaattgttta
     1201 tctcaaattt atttgaaatc ttctctaagc tatatccttg ctttcttagt tcatagatct
     1261 gaactttatc ctcataactc aatttcataa taaaacaccc caaaaagtag attttttctg
     1321 tctaactttt ggggtgcagt tcatcttgta ctgctttttt cttttacgac ttacgctgtt
     1381 tggcttcatg agcaagccct caaagtgaac taataacaaa tcatgagaag tcaaagtgat
     1441 gaaatttctg taacagaagt agagcgaatt gctgaaccat taaatgagta tatctcgaag
     1501 tagaagcgtc tattttgact tttagtctga tagaatggta gtttgaaaat gtaaatgata
     1561 gctactgttc ataaaacata aaaggtaagt acggtgttgt gagaagagct agaaattagc
     1621 acttacctga ttattccaat aacctcaaag atgtgataaa aatttcaact actcttatct
     1681 ataataaagc tacctgttct ttgaaaattg aattcactcc gtcttgatta gcgtgcctgt
     1741 gtaagtaggg actgagagta tttccctgtg aagggcaact ggcacaagac gtgtgtgaga
     1801 attttctaac agacacggag tttatcgttt ccagactcaa acgatgcgac aaactagata
     1861 aaggagttaa tcatgaaggt agtaaacttg tatgatttga aacaaatggg aaataaaggt
     1921 ggttgtacca tccaattgat tcatcacttt ccttttggta tgggcttagg acatctcaaa
     1981 aaagactaca ttgaatttaa acgtgttggt atctttgatg ggaaagcagt agaagttact
     2041 cttcgagaac cttattcgag agatctactt caggttgtca agtcaataaa gtaactgaca
     2101 ccacgagtat aattattaga aacgtggaat cttattaaat tttataataa atatattaaa
     2161 tgaaaaatta ttagatataa acttatgtaa taaaacattg aggaaataat aaaaatctat
     2221 gggtaatgtt ctgaaagtac ttttaggtta ggagagaaat ataatgattt ttgaaaattg
     2281 attcaaaaaa tagcgaattg tagaaaacgc ttaacaaatg gtgtataata gacttccagc
     2341 gaaacaaaat atgctacaat aaacctatga attataaagc aaccttatac tcatctgtct
     2401 tcacaaagca ttctagtcca tctccgatta acgatggact ttatcatctc cttctccagt
     2461 ccttgtatga catcttccaa agttcgaaag gctttattct taaatccacg tttacgaatc
     2521 tctttccaca cttattcaat ggggttcatc tctggtgtgt atggaggaat aaatgcaaag
     2581 ccaatattag tcggaatctt taaggcactt gatttatgcc atatagcatt gtccataacg
     2641 agtaaaatat aatcatctgg ataagcttgt gaaagtttgg ctgacagtgg ttatcaaggg
     2701 ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta
     2761 acagttgaag ataaagccta taaccatgcg ctatctaagg agagaagcaa ggttgagaac
     2821 atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa
     2881 cgttttggat tacgaatgaa tttgagtgct ggtattatca atcatgaact aggattctag
     2941 ttttgcagga agtctattat caaaaatacc atcaggatta tataagattg atacaggaaa
     3001 agttttattt gatggtgtaa atattaatca aatagataaa aaaatattaa gtcaaaattt
     3061 aggagtagtt ccacaggatt cgtttttatt gaacagaagt attcttgata acataacttt
     3121 aaagcacgaa gttacttcac aaaagataga ggaagtttgt aaagcagttc aaatctatga
     3181 tgaaatcatg gctatgccga tgaaatttaa
//
LOCUS       DAYYQN010000105         3112 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYYQN010000105 DAYYQN010000000
VERSION     DAYYQN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.106"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(143..676)
                     /locus_tag="ACHBRQ_001979"
     CDS             complement(143..676)
                     /locus_tag="ACHBRQ_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9593575.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(765..1541)
                     /gene="recX"
                     /locus_tag="ACHBRQ_001980"
     CDS             complement(765..1541)
                     /gene="recX"
                     /locus_tag="ACHBRQ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9593576.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1579..2934
                     /gene="rlmD"
                     /locus_tag="ACHBRQ_001981"
     CDS             1579..2934
                     /gene="rlmD"
                     /locus_tag="ACHBRQ_001981"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9593577.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            2996..3067
                     /locus_tag="ACHBRQ_001982"
     tRNA            2996..3067
                     /locus_tag="ACHBRQ_001982"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3029..3031,aa:Glu,seq:ttc)
BASE COUNT          986 a          559 c          579 g          988 t
ORIGIN      
        1 ttttacgaat tctgcataaa tctctttcta gtaatgtgtt gtaactctgc tataatggat
       61 ttattccttt ttgtatttac acaatttatt ttatactacc aaaaaaacaa ggtcaggatt
      121 ttgttcctga cctttgacaa ctttaccgat tctttagttc tacatagcgc ttgtaccaaa
      181 tgtttacata ggcttctgag aaaggaccac gtccattgtt aatccaatca acaagaattt
      241 tgacatgttc ttttaaaata tagtccaagt catcagaata attcattttg cgtttgtgac
      301 gctcgtattc ttcaacgtcc aagagacgtt tttccccatc tgtaaaaatt ttaacatcca
      361 aatcgtaatc aatatacttc agtgcttctt catccagata gtaggggcta gccatattgc
      421 aatagtaaga agttccatta tcacgaatca tggcaatgat attaaaccaa tatttcttgt
      481 gaaagtaaac aatagccggt tctcgagtga cccaacgacg accatcactt tcggtaacaa
      541 gtgtatgatc gttgacacca ataatggcgt tttctgttgt ttttagtacc atggtgtccc
      601 gccaagttcg gtggagactc ccatcatgct tataactttg aattgtaata aagtcgcctt
      661 cttttggaag cttcataact aaccaacttt ctacaattta taagtttatc atttactatt
      721 gtaccataaa attacctaaa atctgtgaat ttcacttgga aatattaaag atattctcta
      781 agagcgcttg ctatatccga aaaatcgtag ccctttcgtg ctaaaacttg agttaaacgc
      841 tgcttcagtt cgtatccttc atactttcga gcatacttag tatattgctt atcaagttcc
      901 ttgaagatga gttcttgagt cgtttcttgg tcaacttggc tatctaagtc gtcaaaggca
      961 attttagcat cagaataaga gaagcccttg ttagtcaagt tctggataat cttatcttgc
     1021 agggcacgag ctggaagttt tccctcatat tttttcaata gtttattggc tacacgttga
     1081 gcaacttcag aaaaatcaaa ttctttcaag ttctcttcta tagtagattt tgaaatccct
     1141 ttttgagata gtttctgagt cagcacataa ggacccttgt ctcctgaaag ttgattggta
     1201 ttgatgatag cataagcgta ctgaccatca ttaatccact tatcttcttt aagattagca
     1261 ataacttgag aaacgatgtt tttatcaata tcgtattttt tcagatattc tctgacttct
     1321 ttttcagtgc gtgctttaaa ggataagtgg tagagagcga gattcttacc ataagaaaat
     1381 tgagcaaagt cttgaatctc tttcaattcc tcttcgctta tcaccttatc tctcgataac
     1441 ataaaacgaa caattgtatc ttcggtgata tagcatttgt cgccattatc aagctccatc
     1501 aggtagagtc tttttttctt ttcaagtttt gtgattttca tagctctatt ataactcaaa
     1561 atgtgataag ataggggtat gaatctgaaa gtgaaacaaa aaataccatt aaaaatcaag
     1621 cgcatgggaa ttaacggtga gggaatcggc ttttaccaaa aaacattagt ctttgtacca
     1681 ggagctctca aaggcgaaga tatctattgt cagattactt ctattagacg caactttgtt
     1741 gaagcaaaat tactgaaggt caacaagaag tctaaatttc gaattgtgcc atcttgtact
     1801 atttataatg aatgcggagg ctgccaaatc atgcacctgc attatgataa gcagctggag
     1861 ttcaagacgg acttacttca tcaagcgctg aaaaaatttg ctcctgcagg atatgaaaat
     1921 tatgaaattc gtccaactat tggaatgcag gaaccaaaat attacagagc taagttacaa
     1981 tttcagactc gaaaatttaa aaatcaggtc aaggcgggct tatatgcaca aaactctcac
     2041 tatttagtag agttgaaaga ctgcctggta caagataagg aaacccaagt gattgctaat
     2101 cgcttagcag aattacttac ttatcaccag attccaatca cggatgagag aaaagttcta
     2161 ggtgtccgta ctattatggt ccgacgcgcg agaaagaccg gacaggttca gattattatt
     2221 gttacaaacc gccagcttaa tttaactcaa ttggtaaaag agttggttaa agatttccca
     2281 gaagttgtga cagtagctgt taatacaaat acagctaaaa ccagtgagat atatggtgaa
     2341 aagacagaga ttatctgggg gcaagagagt attcaagaag gtgtactcaa ttatgaattt
     2401 tcactatccc ctcgagcttt ttatcaacta aatcctgagc aaacagaagt cctctatagc
     2461 gaagcagtaa aagcgctgga tgttgataaa gaagaccatt tgattgacgc ttattgtgga
     2521 gttggaacga ttggatttgc ctttgcaaag aaagttaaaa cactcagagg tatggatatt
     2581 attccagaag ctattgaaga tgccaagcga aatgctaaaa gaatgggatt tgacaatact
     2641 cattatgaag ctggaacggc agaagagatt attcctcgtt ggtacaagga aggctaccga
     2701 gcagatgctt tgattgttga cccaccacgt acaggtctgg atgataagtt attagatact
     2761 attcttactt atgtaccaga aaaaatggtt tatatttctt gtaatgtttc gaccttggct
     2821 cgtgatttgg tacgcttagt agaagtctat gatcttcatt atatccagtc ggtcgatatg
     2881 ttcccacata cagctcgaac tgaagctgtt gtaaaattaa taaaaaaagt ttaaaaaagt
     2941 agttgacaaa gtttgaaaag actgtataat agtaagagtt gaaaataaca actcaggtcc
     3001 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
     3061 cggactatgg tatgttgcgt caggaccact tgatgaaaaa aagtttaaaa aa
//
LOCUS       DAYYQN010000106         2925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYYQN010000106 DAYYQN010000000
VERSION     DAYYQN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.108"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..535
                     /locus_tag="ACHBRQ_001983"
     CDS             <1..535
                     /locus_tag="ACHBRQ_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HFM9593578.1"
                     /translation="RYRELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLD
                     TPLQEIHDYFAKQLEIFPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSE
                     KAGVRFPNHDRYQFHISIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDY
                     TVFEDMRQFVPYHEKFK"
     gene            560..1627
                     /locus_tag="ACHBRQ_001984"
     CDS             560..1627
                     /locus_tag="ACHBRQ_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9593579.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLSGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMSPEIGVPFVTEQTMILS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1730..2740
                     /locus_tag="ACHBRQ_001985"
     CDS             1730..2740
                     /locus_tag="ACHBRQ_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9593580.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2752..>2925
                     /locus_tag="ACHBRQ_001986"
     CDS             2752..>2925
                     /locus_tag="ACHBRQ_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HFM9593581.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQ"
BASE COUNT         1013 a          395 c          589 g          928 t
ORIGIN      
        1 acgttataga gaattaccat ttatcgataa gtttacttta actccaagga attctattca
       61 catgactgta attgaattgt tgtgccatga aaatcgtgaa acggagtttt ggagcagtaa
      121 tcttcctctt gatacacctt tacaggaaat acatgattac tttgctaaac aacttgaaat
      181 ttttccattg ttggatgaag aaattcatat gcgtgtgact gaaatgggaa aacaaaacat
      241 actagttgaa cctgcagatg aagcttctgc aaagagatta gaagaaattc gtacttatgt
      301 ctcagaaaaa gcaggtgttc gtttccctaa tcatgataga tatcaattcc atatttcaat
      361 tgggtatctt cggattcctc taaccgaaga ggaggaagaa gagtttacta aagtcagagc
      421 agaattaact gaaattttat tagagaagat tccaactatt actgtaaacc gtattgacta
      481 tactgtattt gaagatatga gacaatttgt tccatatcat gaaaaattta aataaaataa
      541 taaaaaagga gaacttatca tgaaaaaaat gaaagtttgg tctactgtac ttgcaacggg
      601 agttgctctt actacacttg ctgcttgctc tggaggttca aattctacga ctgcttcttc
      661 atctgaagaa aaagctgata aaagtcaaga attagtaatc tattcgaact cagtctcaaa
      721 tggtcgtggt gattggttaa ctgctaaagc aaaagaagct ggttttaata taaaaatggt
      781 tgatatccct ggcgctcaat tagcagaccg tgttattgcc gaaaagaata atgcagttgc
      841 agatatggta tttggaattg gtgctgttga ttcaaataaa attagagatc aaaaattact
      901 agtacagtac aagcctaaat ggctagataa aattgatcaa tctttatcag ataaagataa
      961 ttattataat cctgtgattg ttcaaccatt agttttaatt ggggcgcctg atgtaaaaga
     1021 aatgcctaaa gattggactg aattaggtag taagtataaa ggtaaatatt caatttctgg
     1081 gctctcaggt ggaacaggac gggcaattct agcaagtatc ttagttcgat accttgatga
     1141 taaaggtgaa ttaggtgttt ccgaaaaagg ttgggaagta gcaaaagaat atttgaaaaa
     1201 tgcatacact cttcaaaagg gagaaagttc aattgttaag atgttagaca aagaagatcc
     1261 aatacaatat ggaatgatgt ggggttctgg tgctttagtt ggacaaaaag aacaaaatgt
     1321 tgtttttaaa gtgatgtctc ctgaaattgg ggttccattt gtaactgagc aaacaatgat
     1381 tttgagtaca agcaagaagc aagcattggc taaagaattt attgattggt ttggtcaatc
     1441 tgaaattcaa gtagagtaca gtaaaaactt tggctctatc cctgccaata aagatgccct
     1501 caaagagcta ccagaagata ctaagaaatt tgttgatcaa gtgaaaccac aaaatattga
     1561 ctgggaagct gttggaaagc atttggatga atgggtagaa aaagctgaat tagaatacgt
     1621 aaaataagaa ataagagtta ttttttatgt ccaagtctct ctattatttc taaatttaag
     1681 gaagaaataa agagaggctt ttggtttagt aatagaggga gttagtgaga tgattaaatt
     1741 tgataatatt caaattaaat atggtgattt tgttgcaatt gataatctga atttagatat
     1801 acatgaaggg gaattcttta catttcttgg gccttcagga tgtggtaaat caactacttt
     1861 gagagcattg gtaggttttt tagatccatc atcaggaagt attgaagtta atggaacaga
     1921 tgtcactcat ttggaacctg aaaagcgtgg aattggtatt gtatttcaat cttatgcgct
     1981 atttccaact atgactgttt ttgataatat tgcatttggt ttaaaagtta agaaggtagc
     2041 tccagatgtt attaaagcta aagtatcagc agtggcagca aaaattaaga tctctgatca
     2101 acagttacag cgtaatgtat cagaattatc tgggggtcaa caacaaaggg tagcattggc
     2161 tcgtgctctg gttcttgaac ctaaaattct ttgtctagat gaaccattgt caaaccttga
     2221 cgcaaaatta cgtgtagatt tgagaaaaga gttgaaaaga cttcaaaaag agttaggtat
     2281 tactacttta tatgttactc atgatcaaga ggaagccttg actttatctg atagaattgc
     2341 agtctttaac aatggataca tcgaacaggt cggtacacca gtagagattt atcataattc
     2401 tcaaactgaa tttgtatgtg attttattgg agatattaat gttttgaccg atgaaacagt
     2461 ccacgaagta ttactgaaaa atacaagcgt tttcttagaa gataaaaaag gttacattcg
     2521 attagagaaa gttcgattca atcgtgaaac tgaacaagat tttattctaa aagggacaat
     2581 tattgatgtt gagttttctg gagttacaat tcactataca ataaaagttt ctgaaagtca
     2641 gattcttaat gtaacaagta ttgatagtca ggctgctatt agatctgtcg gagaaagtgt
     2701 ggaattattt atcacaccat cagacgttct gcaattttaa ggaggtgcag tatgcgtcat
     2761 aaattaaatt taaaagattg gcttattcgt ttaggattaa tctggttctt agtaacattt
     2821 attatttatc caaactttga tctagtagtg aatgtatttg taaaaggagg agaattttcc
     2881 cttgatgctg tacatcgtgt tctaaaatct cagagggcac ttcag
//
LOCUS       DAYYQN010000107         2898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYYQN010000107 DAYYQN010000000
VERSION     DAYYQN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.109"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            58..600
                     /locus_tag="ACHBRQ_001987"
     CDS             58..600
                     /locus_tag="ACHBRQ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9593582.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(641..714)
                     /locus_tag="ACHBRQ_001988"
     tRNA            complement(641..714)
                     /locus_tag="ACHBRQ_001988"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Asn,seq:gtt)
     gene            complement(720..791)
                     /locus_tag="ACHBRQ_001989"
     tRNA            complement(720..791)
                     /locus_tag="ACHBRQ_001989"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(756..758),aa:Glu,seq:ttc)
     gene            complement(843..1595)
                     /gene="comE"
                     /locus_tag="ACHBRQ_001990"
     CDS             complement(843..1595)
                     /gene="comE"
                     /locus_tag="ACHBRQ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HFM9593583.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1592..>2898)
                     /gene="comD"
                     /locus_tag="ACHBRQ_001991"
     CDS             complement(1592..>2898)
                     /gene="comD"
                     /locus_tag="ACHBRQ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HFM9593584.1"
                     /translation="TVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTEIVFDFPL
                     YILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNFLSVTFSS
                     ITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLL
                     LHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFE
                     QKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHK
                     LRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRI
                     MSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKG
                     RNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
BASE COUNT         1005 a          533 c          417 g          943 t
ORIGIN      
        1 acaagataaa aaatctattt ttagacatat agtctaattt gtttacaagg aggaaatatg
       61 caagaaagta acaaacgctt aaaaacaaag cgaactattg aaaatgctat ggtacaatta
      121 ctgatggaac agccatttga taaaatttct actgtcaaat tagtagaaaa agccggaatt
      181 agtcgttcca gcttctatac tcactataag gataagtatg atatgattga gcactatcaa
      241 agcaagctat tccatacatt tgaatatatt tttcaaaaac atgctcatca caaaagagac
      301 gctattttag aagtttttga atatctagag tcagaaccac ttctggctgc ccttctttct
      361 gaaaatggga ctaaagaaat ccaaaatttc ttacgaaata aacttcatat catgcttagt
      421 acagatttac aaaagcgatt tatgcaactg aatctaaata ccaccgaatt agaatatagt
      481 agcatctatc taactcatgc actttttggt gtctgccaaa cttggattgc acatggaaaa
      541 aaagaaagtt ctcaagaaat aacagacttc cttatgaaaa tgcttggtga tacaaattga
      601 tacaaaaaga ggaacacagc tgtgttccct tttatctata ctccgccagt aggactcgaa
      661 cctacgacat catgattaac agtcatgcgc tactaccaac tgagctatgg cggataaaat
      721 agtccgtacg ggattcgaac ccgtgttacc gccgtgaaaa ggcggtgtct taaccccttg
      781 accaacggac cttctatctg tagcagatat aaccattata tcaatttctt gctaattgtc
      841 aatcactttt gagatttttt ctctaaaata tcttttaatt ttctaatttt taatcttgaa
      901 ataggacaac gatggtcttc atagaaaaca atttctaagt tttttcgatc aatttctctg
      961 atattgccta tatttaccaa aaatgacttg tgaggagaat aaaatcgctg agtatgtttg
     1021 tccttttcct gaatatctgt catggtacca taaaactctt ttgcaaaatt cttaccaata
     1081 atgcgcaatt tatgagagac ccctgttgtt tcaatataca aaatatcatg gtaaggaatt
     1141 tttaaatcat ttcctttgta attgtagtcg aaataatcta caacatcttc attttcaagt
     1201 aacatactct tcgtgtagaa gatattttgc tcaattctct tcttaaacat ctcatcattg
     1261 atatccttat caacaaaatc tagggctgat acctggtatt tataggttag agtcgcaaac
     1321 tctgatcgac tagtgataaa gacgataata gcgtaaggat tgtaatgacg aatgagctga
     1381 gccacttcaa atcccttttt ctcaattcca tgaatatcga tatctaggaa ataaagctga
     1441 tttacttcat cattttcaat gtattcttca aattcacgga cttttcccgt tgtcttgtat
     1501 gatattggaa tattcgattc tttcgaaatt tcatccaata ttctctctag tctcacttga
     1561 tgttcaataa catcttctaa aattaaaact ttcattcaaa ttccctctta aatctaatga
     1621 tttgtctaaa tgtactgcct tccatctctg tttctaaaat aatattgttg tacttatcta
     1681 gtagttcttt cacattattt aatccgactc cgcgatttct tcccttagtg gagaatccta
     1741 aggcaaatag atctcctgaa ggagtcatcg tcattttaca tgaattctga atcacaataa
     1801 ctgtttcagt ttccatctta ataactgcta cttccatctg ctttttatag ctatcagccg
     1861 atccttcgac agcattattc aataaaacgc tcatgatacg aaccaaatcc aatagttcaa
     1921 ttggaagctt ggtaatcgta tcttttactt ccagtgtaaa ctctacacca ttatttcgag
     1981 catagacaat tgactgagca accaaacttc gtaaagctga gtcttctatg ttgttcaaat
     2041 caaagtaagt gtacttatct gaacgcaatt tatgatttgc tttgactaaa acttcattgt
     2101 aaattctgtc aatttcctgt aaattaccac tgtcaattgc catctgcatg ctgacaagca
     2161 ttccagcata atcatgtcga aaaccacgga tttcattata cagaccaaca atttcatctg
     2221 tgtaattctg taaatgtttc tgttcaaatt tcttctgctt caaagcaatc tctttctcca
     2281 tttgaacttt atgagaattc attgcaaaga aggtcaaaag gagagagata aagacaatag
     2341 atgacaaaat acttccaaaa ctattcaaat gtttaatcgt acttaccata tctgaaacga
     2401 aagatacaat atgtagcaat agtaaagcaa aaaatacttt tttcaagaaa ggataaaggt
     2461 agtcctcgtc aaaataggct agttccaaat ggaaatagta aatgattttt aatgtaacaa
     2521 aataggttaa caccgtcaca acgaaaaaga atgggaaatg atattgtaaa acaaaattat
     2581 ctcctgttat agaggagaaa gttacggaca gaaagttatg agtgctctca tataaaagag
     2641 atagtagtaa acttaggaat agtcctctat ccctctcata ctgtttcatc catcgaaaat
     2701 aggaatataa tcccaaagga aataaaaatc tttcaatccc taacccatct aaatatagaa
     2761 tatataaggg aaaatcaaat actatttctg ttagtaatgt ataagcacca aaaacgaata
     2821 attcttttct atttatttga cctttacaaa taaaatggta actgtgacta ataattaaaa
     2881 aatgaacaat aaccgtcc
//
LOCUS       DAYYQN010000108         2869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYYQN010000108 DAYYQN010000000
VERSION     DAYYQN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.110"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..644
                     /locus_tag="ACHBRQ_001992"
     CDS             <1..644
                     /locus_tag="ACHBRQ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HFM9593585.1"
                     /translation="GFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTL
                     IYEMSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSN
                     KGKHQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNI
                     PQSLAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            839..1159
                     /locus_tag="ACHBRQ_001993"
     CDS             839..1159
                     /locus_tag="ACHBRQ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9593586.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            1205..1513
                     /locus_tag="ACHBRQ_001994"
     CDS             1205..1513
                     /locus_tag="ACHBRQ_001994"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9593587.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            1530..2828
                     /locus_tag="ACHBRQ_001995"
     CDS             1530..2828
                     /locus_tag="ACHBRQ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9593588.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPTIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
BASE COUNT          902 a          377 c          591 g          999 t
ORIGIN      
        1 ttggcttttc ttgggggaaa agtttaagta atttagtaga tttaattcac agtaaaagtg
       61 tccgaaatgt tcacttctat cctctagcag gtggtcctag tcacatacac gctaaatacc
      121 atgtgaatac actgatttat gaaatgtcta gaaaatttca tggagagtgt acatttatga
      181 atgcaacgat tgtgcaagaa aataaattgt tagcagatgg tattttgcaa tcaagatatt
      241 ttgaaaattt gaaaaatagt tggaaagatt tagatatagc tgtagtcgga attggtgatt
      301 ttagcaataa aggaaagcat caatggttag acatgcttac agaggatgac tttaaagaat
      361 tgaccaaagt taaaactgta ggagaaattt gttgtcgatt ttttgattca aaaggtaaag
      421 aagtctatga aaacttacag gaaagaacga tagcaatctc tttggaagat ttaaaaaata
      481 ttcctcaaag tttagctgtt gcttacggtg atacgaaagt atcttcgatt ctttctgtct
      541 tgcgtgctaa tttagtaaat catttgatta cagacaaaaa tacaatttta aaagttttgg
      601 aagaagatgg ggatttgact tttagagaga ttctaggtga gtgaaaatga tagactgatt
      661 cagtttatcg tttttctttt tagttgattg cacatttgtg cttatataaa caaaaatagt
      721 ttatctgttg tttttggatt gacaacttta ttatgtagtt gtattctata gttacaaaag
      781 aaaattttaa aatttcaaat gaaaaaagct ttttacatag tgaaatgagg aggaatttat
      841 ggaaattatt gttccagatc aaattatcat gggtttaatt ttatatgctg gcgatgcgaa
      901 acaacatatt tataaagcat tagattacat aaagaacggt acatgtgaac gatgtgaaga
      961 agaaatccag ttagctgatg cagccttatt agaagctcat aatctacaaa caaaattctt
     1021 ggcacaggaa gcgtctggta caaagacaga aattacagct ctctttgttc attcacaaga
     1081 tcatctcatg actagtatga cggagattaa tttaatcaaa gaaattatta gtttgagaaa
     1141 agaacttcat aaaaaataat actagagtat tatcattgtt attaacatag aggaggaaaa
     1201 cataatggtg aagattggtt tgttttgtgc agcaggtttt tctactggta tgcttgtaaa
     1261 taatatgaaa attgcagcgc aatctagtgg agttgaggca gaaatagagg cgttttctca
     1321 gtctaaatta gcggattatg cgccaaatat agatgttgca ctattgggtc cacaagttgc
     1381 ttatacatta gataaatcaa aagaaatttg tgataagtgt gatgttccga tagctgttat
     1441 tccgatgatg gactatggta tgttagatgg gaaaaaagta ttagatttgg ccctatcttt
     1501 gattagtggg taagaaaagg agatttatta tgtcaaagat ggatgttcag aaaatcattg
     1561 caccgatgat gaagtttgtg aatatgcgtg gcattatagc tctaaaagat gggatgttag
     1621 caattttacc attgacagta gttggtagtt tgttcttgat tatgggacaa ttgccgttcg
     1681 aaggattaaa taagagcatt gctagtgttt ttggagctaa ttggacagag ccgtttatgc
     1741 aagtatattc aggaactttt gctattatgg gtctaatttc ttgtttttca attgcctatt
     1801 cttatgctaa gaatagcgga gtagaggctt taccagctgg agttctatct gtatctgcat
     1861 tctttatttt gctaagatcg tcttatatcc ctaaacaagg tgaggcgatt ggggacgcta
     1921 ttagtaaagt ttggtttgga ggccaaggaa ttatcggtgc tatcattata ggtttggtag
     1981 taggaagtat ttataccttc tttataaaga gaaaaattgt tattaagatg ccagaacaag
     2041 ttccacaagc tattgccaaa cagtttgaag caatgattcc agcatttgta attttcttat
     2101 cttctatgat tgtatatatt ttagcgaagt cattgactaa tggcggaaca ttcatagaaa
     2161 tgatttattc tgctattcaa gttccgttgc aaggtttaac tggatctttg tatggtgcta
     2221 ttggaattgc attctttata tcatttttgt ggtggtttgg tgttcatggg caatcggtag
     2281 taaatggagt agtgacagct ctgcttttat ctaatcttga tgctaataaa gctatgttag
     2341 cctctggtaa tctatcatta gaaaatggtg cacatattgt tactcaacaa tttttagatt
     2401 catttttaat tctatcaggt tcagggatta cgtttggtct tgtagttgcc atgctttttg
     2461 cagcaaaatc aaaacaatac caagccttag gaaaagttgc agcttttcca acaatattta
     2521 acgtaaatga gccagttgta tttggatttc cgattgtcat gaatccagtt atgtttgtac
     2581 ctttcattct tgttcctgta cttgcagctg tgatagtata tggagctatt gcaacaggtt
     2641 tcatgcaacc attctcaggg gtaacattgc cttggagtac accagctatt ctatcaggat
     2701 ttttggtggg tggatggcaa ggtgttgtga ctcagttatt agtattggtg atatctacag
     2761 tggtttactt tccattcttt aagatacaag atcgtttagc ttaccaaaat gaaatcaaac
     2821 aatcttagag gtatttgtgt gttactgtta aactcacaca tttgtgcta
//
LOCUS       DAYYQN010000109         2812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYYQN010000109 DAYYQN010000000
VERSION     DAYYQN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.111"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(151..879)
                     /locus_tag="ACHBRQ_001996"
                     /pseudo
     CDS             complement(151..879)
                     /locus_tag="ACHBRQ_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
     gene            complement(1125..1436)
                     /locus_tag="ACHBRQ_001997"
     CDS             complement(1125..1436)
                     /locus_tag="ACHBRQ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HFM9593589.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1451..2737)
                     /locus_tag="ACHBRQ_001998"
     CDS             complement(1451..2737)
                     /locus_tag="ACHBRQ_001998"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9593590.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          821 a          626 c          501 g          864 t
ORIGIN      
        1 aggctttgat acctaagctc cgactttatc acttaaaaaa taaaaaagga cagctaatct
       61 cttgcgcatc aagggatttc gccattcttt aatctcgttt aagtgttgta tatggagctt
      121 tgcataattt tttaaaacta aacaactaca ttaaacacga actgtgacgc tggttccatc
      181 ttccttataa agattgatga gtccttcttt taatgcacga accatgtctc ctgattgaac
      241 gggttgaggc accttaatag tcaatagttc cattggattt ggagcgcggt cgattttatt
      301 gcctttggca tcatgcaaat cttcaatata ggtttcaaaa tgacggaaac ctggaccata
      361 aaactcaact tggtcccctt cgttaatgac atttcgttga cgaatagttg ctgtttgtgc
      421 cgcatcatca taagaaacca cttcagcgac aaacttgtac tcaggaattt tacgacgagc
      481 accaaacaac tgctcatttt cagatggtgt accatagtaa aatcctgtag ccagttcacg
      541 ttgggcaacc ttccacatct cgtccaccaa gtcttgtttg atagcttcaa acttttcagg
      601 actttcaaga taggcatcca cagccgcctt gtagcagttg gttactgtta atacatagtg
      661 aatagactcc atacgtcctt cgatttttag actgtccaca ccattttcaa tcatatctgg
      721 aatgtggtca atcatagaca tgtcaacggc tgacattgaa aattcttctg gaatctcacc
      781 ctgcaaactc ttacgttctt tcccaaatgg catatcgtaa aggtcgtact tccaacggca
      841 tgactgagaa catccaccac ggttggcatc acgcatactc tagacggatt gttttgacaa
      901 ttctaatctt ttagactttg cataataact gttaaacagt tgaaaattga cccagtttta
      961 acaagtagtc aaaataccag ataaaggaaa aaagtcttta aaatcttaaa aacgcatagt
     1021 atcaggtatt gaacaatctt gatattatgc gttttattgt gggaagattt acttcatttt
     1081 ctcttggaat tgagttttct cctacttcaa aactagatga tttgctattt aaaatagcgt
     1141 tccttatgat tttggcaacc tggattaaag cgagaaaaac aaaaggggca acttaagctt
     1201 tctttatatt gcttatatgt tagtagtttt agacaaacac cacataaaat aggcttatcc
     1261 tgtatcaaag ataagggata cggctcaaaa gggtgatttt ctaatgaatc atgacaccga
     1321 taacaagcat aatacttttt acaatcatag cattgtaaag caataatatc cagcttgcta
     1381 tggtaatgaa tgcatcttgt ttgcttatcc actaagaaac cctgtgcctc tatcatttcc
     1441 ttttcctttc ttaagctcga actgtgacgc tggttccatc ttccttataa agattgatga
     1501 gtccttcttt taatgcacga accatgtctc ctgattgaac gggttgaggc accttaatag
     1561 tcaatagttc cattggattt ggagcgcggt cgattttatt tcctttggca tcatgcaaat
     1621 cttcaatata ggtttcaaaa tgacggaaac ctggaccata aaactcaact tggtcccctt
     1681 cgttaatgac atttcgttga cgaatagttg ctgtttgtgc cgcatcatca taagaaacca
     1741 cttcagcgac aaacttgtac tcagggattt tacggcgagc accaaacaac tgctcatttt
     1801 cagatggtat accgtagtag aaacctgttg ccaattcacg ttgggcaacc ttccacatct
     1861 cgtccactaa gtcttgtttg atagcttcaa acttttcaga actttcaaga taggcatcca
     1921 cagccgcctt gtagcagttg gttactgttg atacgtagtg aatagacttc atacgtcctt
     1981 cgatttttag actgtccaca ccattttcaa tcatatctgg aatgtggtca atcatagaca
     2041 tgtcaacggc tgacattgaa aattcttctg gaatctcacc ctgcaaactc ttacgttctt
     2101 tcccaaatgg catatcgtaa aggtcgtatt tccaacggca tgactgagaa catccaccac
     2161 ggttggcatc acgcatactc atgtggtttg aaagagtaca acgtccagag tatgaaatac
     2221 acatagctcc atggacaaag gcttcaattt caacatctgt acgtttgcgg atctcagcta
     2281 attcttccat tgaaacctca cgcgctaaaa cgacacgagt caagcctagc tctttccaga
     2341 actcaagggt ttcatagtta gtggcactgg cttgggtaga aaggtggatt tcaaggcctg
     2401 gtgcttcagt cgctgcaatc ataatcaagg ctgggtcaga tacgataact gctgcaatcc
     2461 cgatatcacg cagtttacgg aaccactcac cagcaccagc ttcatttcct tcgtgcataa
     2521 ccatattagc cgctacatag accttggcac catacttggc cgcaaactgc acgccttctt
     2581 ccatctgttc gaaagtaaag tttcccgcac ggctacgaag accataggcc tgaccaccga
     2641 taaagacagc atctgctcca tactgaacag ctacctttag cttctctaaa gtccctgcag
     2701 gtgataaaac ctcaggacgt tttaatgttt ttgtcataat ttctcctatt tgcaatataa
     2761 aagtttgaca tttttccttc tcattttata gtaaataaag gataaaatca ag
//
LOCUS       DAYYQN010000110         2772 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYYQN010000110 DAYYQN010000000
VERSION     DAYYQN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.112"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..186)
                     /locus_tag="ACHBRQ_001999"
     CDS             complement(<1..186)
                     /locus_tag="ACHBRQ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9593591.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKIKQKLGEKQHSV"
     gene            complement(241..1311)
                     /locus_tag="ACHBRQ_002000"
     CDS             complement(241..1311)
                     /locus_tag="ACHBRQ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFM9593592.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(1328..1708)
                     /locus_tag="ACHBRQ_002001"
     CDS             complement(1328..1708)
                     /locus_tag="ACHBRQ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9593593.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(1721..1984)
                     /locus_tag="ACHBRQ_002002"
     CDS             complement(1721..1984)
                     /locus_tag="ACHBRQ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9593594.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(1984..2217)
                     /locus_tag="ACHBRQ_002003"
     CDS             complement(1984..2217)
                     /locus_tag="ACHBRQ_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593595.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(2217..2585)
                     /locus_tag="ACHBRQ_002004"
     CDS             complement(2217..2585)
                     /locus_tag="ACHBRQ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9593596.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
BASE COUNT          720 a          466 c          554 g         1032 t
ORIGIN      
        1 aacagaatgt tgtttttctc ctaacttttg ttttatcttt tcctgaagta aaatcgcagc
       61 ttgatttctt gcttggggag tttttttctc catcgttact gagacttttt ttaatttctc
      121 agtatatgga tctttgtatc tctcaaaaaa tttatatttg ccgtttggca attcttccat
      181 ccacattgat tttacctcac ttttttgata aaatgggtat agtaaagagg gcttttttta
      241 ttatgagaat ttcttgatta cttctaaaat ttcattggca taatttgcga cttcgagagg
      301 ggtagacacc gggaagatgc ctttatcacg gatttcgata gtgctttttt ttgaatttga
      361 acgataacgt aatacccatt ttttgatgtt atcgtcaacc aaaacattga aatagctacg
      421 gttatctctg taaaatacac gttctggaga aactacatcc ctagcaagca ttttaacaac
      481 agtataaact tctaattcag caggagttgt gataatttcg tcagttactt cgacaatctc
      541 ttcagcttca gcttcaactt ttggaatatc ggttgttact ttggtttcaa cgcttgtatt
      601 aagtgcagca cttaattttt cattaactcg ctctgtgatg aattgattaa accctttcac
      661 aataatggga gaaaatgtcg ttaagatatt ttgagtaaca cgcccttcat agatttcaga
      721 agttagatat ctgaggaagc tatctgacgg tgtagtgata ttttcagtaa ggaacgcttt
      781 aagattgttg aggtatttca attcagaagc tgatgaaaca atattatcaa tatcaaaatt
      841 ctctttgtgg aatttgatga tttcagtaaa ttgattttct ttgatatcag tcacatcgat
      901 tgttaagaat ggagttgtgt ccattttatt tggctcatct aaatcagtaa agaatttata
      961 ttctctacca tttgtcaaga taccgaattt tgatttagta gttacgaaat atctgaatag
     1021 ttgagagtcg tgcttagtaa ggttttctgt aattgattta cattcaatta ggatttgagg
     1081 ctcgccatcc aaaatgatcg catagtcgac tttttcgcct tttttaatgc ccacgtcggc
     1141 agtaaattcc gggacaaatt caagtggatt gaagatatca tatccaagcg cttggaagaa
     1201 cggcataatg aaggcattct ttgtttgttc ttcattcgta atgctttggc tgaggtctgc
     1261 tacacgtttg ccgacttgtt ttaaatcggc ttttacttta tcaatttcca tattgatact
     1321 ccttttttta atttactaat gctaaatact cttctttaac catgatttca tttgtcatgg
     1381 tttttagatt gtagtaagac atgaatttga ggtaatcaaa ctctgtgggg tcgtctaagt
     1441 tttctagagc gtcttttacg agatgatgga tcatattcct atcagcttcg ttttcacagc
     1501 gtagacgggc gttctggtgc tctgagcgtg tgtggtcttt gtgtccaagt tcgtgtaggg
     1561 cgacttggat ttgttgttca ggagtcaagt tgtggtcaat agcgagcacg tttgtgtctg
     1621 gattgtagaa accgctattg tgccagtttg aaccgtcgaa gagacaaagt tctacttgat
     1681 attcttcgca gagtttagcg agtgtcatag ttctccttcg ttatttatag acatctctgc
     1741 ggtgggcgat ttccacggct aggacgacta gtttatcgtc ttggatatca cagatgatgc
     1801 ggtaattctc tactctgtat cgccagtaac ctgcaaggtt ggcttttagt gcttttccgt
     1861 gttgtcgtgg attggtcgtg ttttcaatgt ttttagcaag ccaggatagg atttgttttc
     1921 tggttggggt atctagtttt ttaagttgct tgagagcttt tttatcaata tctagccgat
     1981 acattaagca atatcctctc gagttagtcc tagttcatcc aagacctcat ccatggtata
     2041 agtagctggg tcggctaaat actccatata ggcttggtcg gctgctcgtg cgtcttcgat
     2101 atcttccatg agtgccatga agtcgtcaaa atccatggtc gttgtgtcga taccgtgttt
     2161 gtttaggtag tccgtaatgt aggaattttt ttctgtgaag ttgatagtga tagtcattag
     2221 cgttctcctt tgcttttgaa gtgggcggat aggacggatg taatgaagtc gatatcatct
     2281 tcatttagtg gtttcccgtc aaataacatg gtgttggctg ctgctttgcg taagtctatg
     2341 atttgtccgt ttacttgggc aaattcatca ctcccagcga tagcagggtt atctgttcgt
     2401 ccgagcaggt agtcggtgga tacgttgaaa taattagcaa tttctgcgat acgttcagcg
     2461 tttggcgtag aattttttat cttatagagt gtatttctac cataacctaa gtcttcttct
     2521 acttgtccaa gagcttttcc acgctttttt gccaattctt ttattttttc aaatgtctca
     2581 aacattgtta aatcaacctt tctaagacat cacaaaaaaa atttaacaaa tttggtgtaa
     2641 aaaggttgac taattatccc aaaaggtgta aaatgttttt tgtaagtgat aaacaactaa
     2701 aaaaacaact aagaaataaa ttataaaaat gttttggcga acggtattta tagatttatt
     2761 ggtgttttta tt
//
LOCUS       DAYYQN010000111         2609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYYQN010000111 DAYYQN010000000
VERSION     DAYYQN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.114"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(108..1118)
                     /gene="galE"
                     /locus_tag="ACHBRQ_002005"
     CDS             complement(108..1118)
                     /gene="galE"
                     /locus_tag="ACHBRQ_002005"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9593597.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIEAAIHFAGYKAVGESVQKPVMYYENNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGVHNHSPLIETMPTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPFIAQVAVGKRPELSVFG
                     DDYDTVDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKVPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            complement(1131..2606)
                     /gene="galT"
                     /locus_tag="ACHBRQ_002006"
     CDS             complement(1131..2606)
                     /gene="galT"
                     /locus_tag="ACHBRQ_002006"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9593598.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
BASE COUNT          781 a          550 c          363 g          915 t
ORIGIN      
        1 agcgctttca aacatttttt taaaaaaatt tatctttcat taaatatgca tttcattaaa
       61 aggttaagta atttttccaa aatacagcac tcatatagac agtatcatca attatagcca
      121 ttgggatttt ttgattgcca attccatgta tctctacaca tgtcttcaat cgattttgtt
      181 gtcctccaat ttaattcctt atatgctttg tctgcatttg cataacaagt cgcaacgtct
      241 cctgatcgtc ttggtacaat tttatacgga attggaacct tattaacact ttcaaatgta
      301 tttacaagtt gtaatacact tgtgccttct cctgaaccaa ggttataaat ataaacatct
      361 gttttttcag atactttttc taaagctttt atatgcccta tcgctaaatc cactacatgg
      421 atataatcgc gaacaccagt accatctacc gtatcataat catctccaaa aacacttagc
      481 tctggtcgct tacctaccgc tacttgtgca ataaaaggca ttaagttgtt aggaatgcct
      541 gatggatctt ctccaatcaa ccccgactca tgagcaccaa tcggattgaa ataacgaagc
      601 aacgcaatac tccactctga atctgctaca tgaacatctt ttaaaatttg ttcaagcatc
      661 acttttgtgt acccatacgg attcgttgca cttgtcggca tcgtctcaat aaggggagaa
      721 tgattgtgaa cgccgtatac agtcgcactc gatgaaaata caatcttttt aacattaaac
      781 tctgacatca cttcaacaag agctaatgtg ctcataatat tattttcgta atacattaca
      841 ggcttttgca cggattcgcc gacagctttg tatcctgcaa aatgaattgc agcctcaata
      901 gattcttgtt caaaaacttt tctcaatgct ggtttatcac aaacatctaa ttcgtaaaac
      961 acgggacgta ttcctgtaat tgcttcaata cggtctagca ccaagatgct agagttcgaa
     1021 aggttgtcga caatgatgac ttcctttcct aaatttagta attctactac ggtatggcta
     1081 ccaatataac cagctccgcc tgttaccaat attgccattt ttgtttcctc ctaattaatt
     1141 ccaaccgact taacaaatct cataaacgct tcatgcccag acggtgtatt cttataaact
     1201 cctgcatctt ccagaactct cgcaaacact tgtcctgctt cgtgttgaac tacgctatta
     1261 acctcttctt tattaatgcg aggatatttt tctttcaatt ggtcggccca ttctaaatga
     1321 taatccgcaa ttgcattatc ctctcctaaa agatattttc caacttcttc taactctggt
     1381 ttcaaacgag gtggtaatat cgcaagtccc atcacttcga ttaacccgat attttccttt
     1441 ttaatatgtt gtacatcttg atgaggatgg aaaacaccat ctgggtattg ttcagtagta
     1501 tgattatctc ttagaacaat atctaattcg tatctcccgt ccactttacg agcaatagga
     1561 gtcaccgtat ggtgtgggac atcttcagtc atagcaatga tgtctacttc taaatctgaa
     1621 tattctctcc acttatttag aattttagta gctaaatcta acaagcgatt tttattttca
     1681 ctttgtaacc taattactga cattggccat tttacaatac cagcattaac atcctcaaag
     1741 tctttaaaac aaaattcact ctcaaatttt gctttttcca ttgggaaaat atgtttccct
     1801 ccctggtagt ggttatgact aagaatggag cctcctgaga taggaagatc agaatttgaa
     1861 ccagcaaaat atcctggcaa aatatcaaca atctccaata attgttcaaa tgttttagag
     1921 gtaatagcca ttggtacatg ttgactattc aaaaatatcg catgctcatt aaagtatgag
     1981 tagggagaat actggaatcc ccatacttcg tcaccaagtt tcaaccgaat aattctatga
     2041 ttcgaacgtg ctggataatt tattcgcccc tgatatcctt cattttccat acatagtaaa
     2101 catttgggat aattagttgc ttttactaat ttttcagcag caattgtttt tggatctttt
     2161 tcgggttttg acaaatttat cgtaatctct agctctccgt atttagttga tgctcgaaac
     2221 tcaatattct tagcaatagc agaagtttta atataatcac tatctttact taacttataa
     2281 aactcttcaa ctgcttcttg aggtgatata tcatatgaac tccaaaaaat atcatttaat
     2341 cgactaggta aaggaactat gaaattcatt aactctgctc ctaaacattc cttttcctcg
     2401 attaaatctt taattttacc gttttttaag gcgatttcca ctaagtaatc ttttatttgt
     2461 ttcaggtcat tttcatcgga aatgcgatca attccctcct cacctattaa cgctagtact
     2521 ctatttttca catatatttt gtcaatttca ttatacattc cgtattcaat tactctatca
     2581 acaaaattat caataattgt tttcatata
//
LOCUS       DAYYQN010000112         2102 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYYQN010000112 DAYYQN010000000
VERSION     DAYYQN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.116"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..1062
                     /locus_tag="ACHBRQ_002007"
                     /pseudo
     CDS             1..1062
                     /locus_tag="ACHBRQ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1178..1981)
                     /locus_tag="ACHBRQ_002008"
     CDS             complement(1178..1981)
                     /locus_tag="ACHBRQ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFM9593599.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYENATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          736 a          386 c          383 g          597 t
ORIGIN      
        1 attcataaag tctgacatga aggctggact aaagatagag tttctcttga atacggtctc
       61 ccaagtcgta cgatacttct taactggcta gcacaataca ggaaaaacgg gtatactatt
      121 gttgagaaaa caagagggag agtacctgag agcggagaat gccatcctaa aaaagttaag
      181 agaactccga ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt tcaagaatta
      241 atgactgagt tttcgttagt tcttcttcta aaagccatta aactagctcg ttggacctac
      301 tactatcact tgaaacagct agacaaaaca gataaggacc aagagcttaa agctgaaatt
      361 caatccattt ttatcgaaca caagggaaat tatggttatc gtcggattta tttagaacta
      421 agaaatcgtg gttatctggt aaatcataaa agagttcaag gcttgatgaa agtactcaat
      481 ttacaagcta aaatgcgaca gaaacgaaaa taaaggagac gttggtaaga aggcagagaa
      541 tctcattcaa ggccaatttg aaggatctaa aacaatggaa aagtgctaca cagatgtgac
      601 agaatttgcc attccagcaa gtactcaaaa gctttactta tcaccagttt tagatggctt
      661 taacagcgaa attatcgcct ataatctttc aacttcaccc aacttagaac aagtacaaac
      721 aatgttggaa caggcattca cagagaagca ctacgagaat acgattctcc atagtgacca
      781 aggctggcaa taccgacacg attcttatca tcggttccta agagtaaggg aattcaagca
      841 tctatgtcac gcaagggaaa cagccaagac aacggtatga tggagtcctt ctttgggatt
      901 ctgaaatcgg aaatgtttta tggctatgag aaaacattta aatcacttaa tcaattggaa
      961 caagctattg tagactatat tgattactac aacaataaac gaattaaggt aaaactaaaa
     1021 ggacttagcc ttgtgcaata cagaactaaa tccttcggat aaattatttg tctaactttt
     1081 tggggtcagt acacctaaaa ctttgatgat atacgttttc ttgtgagaat atttacttca
     1141 tttttgccta aaattcaatg tttactcagt atttggatta tgaaaaatcg aggtctaaat
     1201 ctagatacat tttttctgaa gacaaatcat tttgaccacc gagcaagaga ttttcaaaaa
     1261 aagctgttaa aaactcagaa cgtcgctgta aaatctttgc attatctaat accaaggcat
     1321 cacgaaaata tttggaatgt tgctgaaatg gtgtattatc aatatcaaaa ccaaactcac
     1381 gaagatactg aatcaaaaag accgttactg tccgagtgtt tccttcgcga aatggatgaa
     1441 tctgccagat tcctgaaata aaatgctgga tttgtttaac cacatccgcc tgagttagtg
     1501 tcgcatatgc aacttgtttt tcctgattaa aatcataatc taaggtcatt tgaatcatgg
     1561 agtaatcaga gtacacaaca ctttcaccat tcaaaacagg ttcattcttt gtgatattgg
     1621 tctgacgaaa ttgccccacc ggaatagagg gttcaaatat atcttgaaac aactccttat
     1681 gaatagcaag taaggtcgca ggactaaagc taaagcctct tcgagacaat agttctacaa
     1741 tacgtagaga aaccaagtct gcctcttccg tacttgcatc aatagtatgg tgataagccg
     1801 ttgcattctc ataaacctgc tcataagtca gttctccccg ggactgtttc tcagccaaag
     1861 attccatata cgctgatggc actagattgt caactttctg cagaccaaaa cctatccgcc
     1921 ataaatcacg cttcgcttca taagacaagt ttggattgtc aatgttgtaa gttgattgca
     1981 tagaaatatc ctttcaattg tttttcttca ttaccagtaa atcacgaaat tggttggaaa
     2041 ctggcttaaa ctataaaaag tcctcacaat aggttctatg atatatatat atacttcatg
     2101 ta
//
LOCUS       DAYYQN010000113         1873 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYYQN010000113 DAYYQN010000000
VERSION     DAYYQN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.119"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(105..818)
                     /locus_tag="ACHBRQ_002009"
     CDS             complement(105..818)
                     /locus_tag="ACHBRQ_002009"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9593600.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1276..1656)
                     /gene="dhaM"
                     /locus_tag="ACHBRQ_002010"
     CDS             complement(1276..1656)
                     /gene="dhaM"
                     /locus_tag="ACHBRQ_002010"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9593601.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
     gene            complement(1656..1730)
                     /locus_tag="ACHBRQ_002011"
                     /pseudo
     CDS             complement(1656..1730)
                     /locus_tag="ACHBRQ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
BASE COUNT          614 a          375 c          291 g          593 t
ORIGIN      
        1 atgtaaattc ataattaaca gcttcattaa aatcaacttc agacatttca atgtactctt
       61 caatagtaat attagctttt tttgtagtca ttagctcatc tcctttaaaa tgtgatcttt
      121 tcaagaatat ttttatctaa acaatccagc aagtcttggt aagaatagac ttcgtaagtc
      181 ggctgggctt gtgtgtgatt ttcgaggtga tgaggattat accagatagt gtcaatcccc
      241 gcattattgc caccttgaat gtcggcggtt agagaatctc caatcatcag cgtcttttct
      301 ttactaaatc cagcaatttg ctggccaatc ttttcataaa agagagcatc cggcttttga
      361 gtttgcaact gttctgagat aaagacttga ttgaaataag gtactagacc agattgagcc
      421 aaacgtcctg tctgaatggc agtaatgcca tttgtcgcag catacaagtc ataatcacgc
      481 tcaatgaggc tgtccaagag atcatgagcg cccgatagtg tttgtccctg ctgagcgaga
      541 taaaattggt aacgctgggc aagaaaacta ccgtcttttt cctgtccaaa atgagaaaat
      601 aaacgagaaa agcgcgtgtt aaccaactct tgtttactga ttttcttcag ctccaagtct
      661 ttccagagag ccttgttcat aggaacgtaa taatctttat aagcctgaat atccgcaact
      721 ccttcttctt ttagaagttg ggtcaaagcc acatcctcag cagcatcaaa atcaagaaga
      781 gtgtggtcga ggtcgaagag tagaaatttg tagaacaatt tgaggttttc ctttctgaaa
      841 attcattaag aacattatat cataaagcac ctcatacaat taactaattt aatcacttaa
      901 aaaaattcga acactttcta tacaactgac agctcaaatc tttcagaata gaacaatact
      961 aactatcgaa caccccgtct tcataaatac atatgtaatt ctagacctag aattcctata
     1021 aactaaatgc tttcatactc ttccaagtaa ttgattgcct taaattttaa tttttgaagg
     1081 attctaaagc tagaatagcc ccatcacaat cagttttgat tgattcacaa tttagaaaca
     1141 ctatagtttc actcctgtta aaataaaaag gaactgcata aagcaatccc tttctgattt
     1201 tgaaatcatt tacttaacat tttatagttg agataatcaa tagcttatct ataaaaagag
     1261 ttatagtaaa attccttatt tattgatttc aagctccgct aactgtattt gaataactga
     1321 cagttctgca ccagcctgaa gaagagcagc tgcattatag gcaccttcta caattggaac
     1381 cctgttgatg atgatacttt tatcactgaa atcagtcacc atttttaagt tcattttagc
     1441 agaacctagg tcaaaaaagg caagtaaagt atctgctgga ttttcggaaa caaccctatc
     1501 tacttgatca aaactcgttc caattcctcc gccctcggtt cctcctacat aagtaatcgg
     1561 aacatcttta gctactttac taatcagttc aacaacacct tctgcaatat gtttggaatg
     1621 tgaaacgata acaagaccaa taccaatact ttccatcaaa ccactccagt ttctaaaata
     1681 gcagtaaaga gtaatcctga tgagaatgat ccaggatcaa tatgtccaag aaaccacatg
     1741 ctcctaagac aagagctaac agactggcca tcaataatag tattgttctt tttttcatca
     1801 ttactcctta actagtgttt aactgattaa ttagccagta aatagtttat ctttatttac
     1861 actatctgtt aag
//
LOCUS       DAYYQN010000114         1455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYYQN010000114 DAYYQN010000000
VERSION     DAYYQN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.126"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(201..>1455)
                     /locus_tag="ACHBRQ_002012"
     CDS             complement(201..>1455)
                     /locus_tag="ACHBRQ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HFM9593602.1"
                     /translation="YWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSL
                     EKTKKRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVI
                     KNLYYMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEA
                     PMVHLPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANL
                     KNLAGINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKK
                     DSPDMMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTR
                     ATGYTMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQR
                     EIEVAQEDPEYTDLLLKKALEFFEF"
BASE COUNT          475 a          292 c          241 g          447 t
ORIGIN      
        1 cacattgtgt actatttttg gttcatttta ctatatttta gaagttatag tgtagcattc
       61 caacttcaaa gcactataaa gtaaattgaa acaagaacaa tacaaacaat tctcgtaaac
      121 ggattgcaac cacaaaaaag caagcattca caagaatact tacctatcat gggaggaaca
      181 accgttcctc ttttttatta ctaaaattca aagaattcca atgctttttt caagagcaaa
      241 tccgtatatt ctggatcttc ttgggctact tctatttccc gctgaacttt ttccaaatca
      301 tctgtaatca ctccatctac tcctaagtga agagatttgc tgatagcttc tgaatcattg
      361 acagtccaga cataaagttt ctgatccgtt gtccatagtt tgcttacaaa atattcatcc
      421 aaggttgagt actccatagt atatcctgtc gctcttgttt taggaaagac agaattgtag
      481 ggcatgatga aataaactgg cagttcggca tcatactgtc ttactttttc gacaacatgg
      541 tagtctaaag actggatttg atgtccataa atcttgagct ttgcagcata acgggctaaa
      601 aagcggttca tcatgtctgg actatctttt ttactggttt taatttcaat tagtaatttt
      661 tgaccaagtt cgttggctcg actgagataa tcttcaaagc ttgaaatttt agtctggtag
      721 ccattttcaa aaatatcaat ccctttaagc tcctccaagt ttaagtcttg aggactttta
      781 ttgatacctg ctagattttt caagttagca tcatgcatca tgacaaactg cccatctttt
      841 gtttcctgca cgtccgtctc caccaagtct ggtttgagtt gtgctgtagt ttccaaggac
      901 tctactgtat tttgaatccc atttgcattg gaaacccctc ggtgagaaat aagttgaggt
      961 agatgaacca tgggagcctc cagataaata taaccttcta aggcaaagaa aagactggca
     1021 caagtcatga caccccatcg cacgatgtga tctttttctc tcctaggaag catatccagc
     1081 tcctttcctg tcaaaaatga aacaaattta accaaaaaat aagtcagagc catataatag
     1141 agatttttaa tcacgacaaa attcaaaata ccaagaatca gagactctct ctgagtgata
     1201 tcatctacca aagtttgagc caataataaa ggaatcaaag gaagataaaa taataaatgt
     1261 gctttgagca agatgtaaaa taaattccaa gcataaaaag taactctctt cttggttttc
     1321 tccaagctaa acatcactgc ttctcgaaca gtcagctgat catatacaat cttcggaagg
     1381 gcaaacatca atctgacaga gacatagaga aagataagag atagaagtag gatgctcagc
     1441 caccacatcc aatat
//
LOCUS       DAYYQN010000115         1432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYYQN010000115 DAYYQN010000000
VERSION     DAYYQN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.127"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            42..1388
                     /gene="gdhA"
                     /locus_tag="ACHBRQ_002013"
     CDS             42..1388
                     /gene="gdhA"
                     /locus_tag="ACHBRQ_002013"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9593603.1"
                     /translation="MTSAKEYIQSVFETVKTRNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          428 a          286 c          309 g          409 t
ORIGIN      
        1 atgtcttatg taataaaata tgacaataaa aggagaacga tatgacatct gctaaagaat
       61 atatccaaag cgtgttcgaa actgtaaaaa ctcgtaacgg gcacgaggct gaattcctcc
      121 aagctgttga agaatttttc aacactttgg aacctgtatt tgaaaaacac cctgagtata
      181 tcgaagaaaa tatcttggca cgtattactg agcctgagcg cgtggtttct ttccgtgttc
      241 cttgggttga ccgtgatgga aaaattcaag taaaccgtgg ttaccgtgtt caattcaact
      301 cagctgttgg accatacaaa ggtggacttc gtttccaccc aactgtaaac caagggatct
      361 tgaaattcct cggatttgaa caaatcttta aaaatgtctt aactggactt cctatcggtg
      421 gaggtaaagg tggatcagac ttcgatccta aaggtaaaac agatgctgaa gtgatgcgct
      481 tctgccaaag cttcatgact gaattgcaaa aacacatcgg accatcactt gacgtacctg
      541 ctggtgatat cggtgttggt ggacgtgaga ttggttacct ttacggtcaa tacaaacgtc
      601 ttaaccaatt tgatgctggt gtcttgactg gtaaacctct tggatttggt ggtagcttga
      661 ttcgtccaga agcaactggt tacggtttgg tttactatac tgaagaaatg ctcaaagcta
      721 acggtaacag ctttgctggt aagaaagtcg ttatttcagg ttctggtaac gttgctcaat
      781 atgctcttca aaaagcaact gaactcggtg caactgttat ttctgtttct gactcaaatg
      841 gttatgtcat cgatgaaaat ggtatcgact tcgatctttt ggttgatgtt aaagaaaaac
      901 gtcgtgctcg tttgactgag tatgcagctg aaaaagcaac tgcaacctac cacgaaggta
      961 ctgtatggac ttacgctgga aactatgaca ttgctcttcc atgtgccact caaaacgaaa
     1021 tcaacggtga agcagctaaa cgtttggttg ctcaaggcgt tatctgtgta tctgaaggtg
     1081 ccaacatgcc gagcgacctt gatgccatca aagtttacaa agaaaatggt atcttatacg
     1141 gacctgcaaa agctgccaac gctggtggtg tagccgtttc agctcttgaa atgagccaaa
     1201 acagtcttcg cctctcatgg actcgtgaag aagttgatga ccgtctcaaa gacatcatga
     1261 caaacatctt caacacagct aaaacaactt cagaaacata cggtcttgat aaagactacc
     1321 ttgcaggagc taacattgct gcctttgaaa atgtagcaaa tgccatgatt gcacaaggta
     1381 ttgtttaaga tttatttact caatcctcaa tcactacgat tggggatttt ta
//
LOCUS       DAYYQN010000116         1306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYYQN010000116 DAYYQN010000000
VERSION     DAYYQN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.129"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(<1..230)
                     /locus_tag="ACHBRQ_002014"
     CDS             complement(<1..230)
                     /locus_tag="ACHBRQ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9593604.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQILDLDYEDLFR"
     gene            complement(486..623)
                     /locus_tag="ACHBRQ_002015"
     CDS             complement(486..623)
                     /locus_tag="ACHBRQ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HFM9593605.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            complement(825..>1306)
                     /locus_tag="ACHBRQ_002016"
     CDS             complement(825..>1306)
                     /locus_tag="ACHBRQ_002016"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFM9593606.1"
                     /translation="LYGSSLISFLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLC
                     FSLIHAQESTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVA
                     LTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
BASE COUNT          447 a          292 c          172 g          395 t
ORIGIN      
        1 ctccgaaata aatcttcata atctaaatct aatatctgca caatcctttc tacccatgga
       61 ctttgaggca ttcgttgttc catcttgtag tggcgaatct tttgatataa acgattcaat
      121 tcacttggat agtgaaactc tcccgcaaac atttttctgg ttaactcaat ccagctgata
      181 tttctttcag ccaaaataat ggacaagttc tcccaaaatc gttcagccat attacttctc
      241 ctttagttaa atcttgaccc gttaaatttg tactgaataa actaaatgaa acttttatcc
      301 cactcactaa tactattata tcaacccact acagttgaca aaaagacaaa ccagcctaat
      361 gactagcttg tcttcaatca agagaggatt ctgttccccc atataagcac taacattcca
      421 acgtgcacat attggaacga catccataac tccagagaat ctctaaagtt tacaatttaa
      481 atgaattaac aattttccca actaaaagca ctccagttac cgcaacggtt tgtactgaat
      541 gtactaaatc gcattccatc aacttcatct gtttcgtcaa cttgaacaga tactaattga
      601 agatttaata cttcttctgc catagctaac tcctcctatt taaatttttg ggattaagtg
      661 ctttatccac cttcattata ctctctccag cagtaaaatg caagcaatga tacaatgttg
      721 tcacgtagaa aataatgttt ccgtaacttt tcaaagtaac ttccatctct ctcccaaaac
      781 tggaagttag ttttagaagt tacctaaaaa tcaggttacc tattttaaaa aagcagcaaa
      841 ctataaacta gtaggctcca caccaaatgt agccccatac tgccccataa gtccaatttg
      901 tagcgtacaa gccctaaaaa catccccagc gatacataca aacaccaagc tataatggtt
      961 cctggatgat gtgctaaggc aaataaaaca cttgtcaaag caactcgaat atctaatttt
     1021 ctaaccaagt tccataaaat ttctctatac agaaattctt caaccatact cgcattgatt
     1081 aaaaacaata aaaatgaaaa ccaaggaact tgatgttgaa gtccaattaa gtttgcttga
     1141 ttcgtgcttt cttgcgcatg gattaggcta aaacatagac ttataatcag taggctaacg
     1201 aatccaatac caagccattt catcctagat ttcatattta ccttatgagc ttgtttgcgt
     1261 tggccataca tccataaaaa agaaatgagt gacgaaccat agagaa
//
LOCUS       DAYYQN010000117         1285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYYQN010000117 DAYYQN010000000
VERSION     DAYYQN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.130"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            21..860
                     /locus_tag="ACHBRQ_002017"
     CDS             21..860
                     /locus_tag="ACHBRQ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9593607.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            967..1242
                     /locus_tag="ACHBRQ_002018"
     CDS             967..1242
                     /locus_tag="ACHBRQ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9593608.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          422 a          203 c          279 g          381 t
ORIGIN      
        1 aaaatagaaa gattgatact atgacaaaaa ttaagattgt aaccgattca tctgttacta
       61 ttgaacctga actagtaaag caattagata ttacaattgt tccattatct gtaatgattg
      121 ataatgttgt ttattctgat gcggatttga aagaagaagg taaatttctt cagttgatgc
      181 aagaaagtaa gaatcttccg aaaacaagtc agccacctgt aggtgtcttt gctgagattt
      241 ttgaagacct atgcaaagat ggtggccaga ttcttgctat tcatatgtcc catgctcttt
      301 cgggtacggt agaagcagca cgccaaggtg ctagcctatc tactgcagat gtgactgttg
      361 ttgatagttc cttcactgac caagccctga aattccaagt tgttgaggct gcgaagttag
      421 ctcaagaagg taaagatatg gaggcaattt tatctcatgt agaagaggtt aaaaaccata
      481 cagagcttta tattggtgtt tcaactttgg aaaatcttgt caaaggtgga cgaattagcc
      541 gtgtaactgg cttgttgagc tctcttctca atatccgtgt tgtcatgcaa atgaaagacc
      601 atgaattgca gccaatggtt aaaggtcgtg gaactaaaac atttaaaaaa tggttagatg
      661 agttgataac atcgctttct gaacgtgctg tagcagagat tggaatttca tattctggta
      721 gtgatgattg ggcaaaagag atgaaagaaa gcttacaagc ttatgttgaa aagccaattt
      781 ctgttttgga aacgggttcc attattcaaa ctcacacggg tgagaacgct tgggctattt
      841 tgatacgtta ccactcctaa aaaaataaat aaaatgggaa gaaatagcgt ttttaacttg
      901 acctaaaagg gattttagga tatgattata tttgttaatt agaaattatt tggaggaatc
      961 attaacatgg caaacaaaca agatttgatc gctaaagtag cagaagctac agaattgact
     1021 aagaaagact cagcagcagc agttgaagct gtatttgcag cagtagctga ctatcttgca
     1081 gctggtgaaa aagttcagtt gatcggtttt ggtaactttg aagttcgtga gcgcgcagaa
     1141 cgtaaaggtc gcaacccaca aactggtaaa gaaatgacaa ttgcagcttc taaagtacca
     1201 gcattcaaag ctggtaaagc tcttaaagac gctgttaaat aatcagtctt taaaaagcct
     1261 attgtatcaa gctttctagc ttggt
//
LOCUS       DAYYQN010000118         1202 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYYQN010000118 DAYYQN010000000
VERSION     DAYYQN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.131"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            18..899
                     /locus_tag="ACHBRQ_002019"
     CDS             18..899
                     /locus_tag="ACHBRQ_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9593609.1"
                     /translation="METVVLQEILNIQNPEDFYVKLNKIDQFGIDTKSLYINDQPKLL
                     EQMGYLYDTLRKTNKPHFNYVMDRPYTIHLIPYDEANKLWLFVGAYSQSGTYQQTYED
                     RVTTYYKLNLAPEHSKLKGRLIVKFERPDGSQHVRIGLESATAQGFKLHSILEREISS
                     VEFQDYRNVRLTYQELKSIIKNQNPTWKTALSHLNAIYLQTDTKTGKQYVGSAYGKQK
                     LWGRWTEYVETYHGVNKALKELFKKEGASYFEDYFTYMLLEVLPSDNKEIGNTVISRE
                     SWWKIALQTREFGYNCN"
BASE COUNT          432 a          193 c          223 g          354 t
ORIGIN      
        1 attgaaagga atttaaaatg gaaacagtag tattgcaaga aatcttaaac attcaaaacc
       61 cagaagattt ttacgtaaaa ttgaataaaa tcgatcaatt tggaattgat acgaaaagcc
      121 tttatattaa tgaccaacca aaattattgg aacaaatggg gtatctctat gacactttgc
      181 gtaagactaa taaacctcat tttaactatg taatggaccg cccttatacc attcacctaa
      241 tcccatatga cgaagctaat aaattgtggc tctttgtagg ggcttactct caaagcggta
      301 cttaccaaca aacgtatgaa gaccgtgtaa cgacttatta caagttgaat ttagctcctg
      361 agcacagtaa gttgaaaggc cgtttaattg ttaaatttga gcgtcctgac ggttcacaac
      421 atgtacgaat tggtttggaa tctgctacag ctcaaggatt taagctgcac tctattttag
      481 agcgtgaaat ctcaagtgtt gaatttcaag attatcgaaa cgttcgccta acttatcaag
      541 agttgaaaag cattattaaa aatcaaaacc ctacatggaa aacagctttg tctcatttga
      601 atgctattta ccttcaaaca gatacaaaga caggaaagca atatgtaggt tctgcttacg
      661 ggaaacagaa gctttggggg cgttggacag agtatgttga aacctaccat ggtgtaaata
      721 aggccttgaa agaactcttt aaaaaagagg gtgcaagtta ttttgaagat tacttcactt
      781 atatgcttct agaagttctt ccaagcgaca ataaagaaat tggaaataca gtaatcagtc
      841 gcgaatcttg gtggaaaatt gctcttcaaa ctcgtgaatt tggatataac tgtaattaaa
      901 tgcgaatact tgctcaacaa ttatgtaata tactaatcga taaaaaacca acaaaataaa
      961 gagcctgaaa caaaatagtt ctcaatcaca taaaagctag agttccgcca actggaaagc
     1021 tctagctttt ttataactga ggaccatttt gtcttagaca ctttgtaagt ataatgagaa
     1081 tattgtttat tgaggtgttt taaccatcta gtctagagtt agactaacgc tagatagtgt
     1141 agaaaggaag agggagttaa actattgttt tacgaatata taatgtgtat gaaaggatac
     1201 ag
//
LOCUS       DAYYQN010000119         1178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYYQN010000119 DAYYQN010000000
VERSION     DAYYQN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.132"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(488..1060)
                     /locus_tag="ACHBRQ_002020"
     CDS             complement(488..1060)
                     /locus_tag="ACHBRQ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593610.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          363 a          210 c          189 g          416 t
ORIGIN      
        1 attctattta tccaagcgat tcaacatttc ttgcttcttc gcttcaagtt ctgcacgctt
       61 ttcttcgatt tcggcatgtt ttttctcgag ttcagaacaa cttgcaccat tgctaaattc
      121 ttttcgccat caggagatag ggtgagtcga catgtctatt actcacccaa agcagtccta
      181 caaagcagga attttctgtt acttttttgg aaatagtaac gtttatacag ctttgacact
      241 tcgtatcaaa gcgccaaaca cactccgagg gatttacaga aagcagaaaa ggaatgatct
      301 ggtataagat cattcctttt ctctctttct ctctttttct ttaagtaatt atatacaatg
      361 tacgacgaag tcgtcattgc aatgctgatc caccacctaa agggaacttt aaacaacatt
      421 gataagataa agaatataaa caacgaaaat acgttatacc caattaattt tattgtatat
      481 ctcatgatta aaagttaatc cttccgttgt taggaatggc atcattttta tcccataatt
      541 gtgctaaata agtccccggt gataataaat tcatagcgaa ttctaaagca acatcattta
      601 caaaccaact acctagatat ctagaaattg ctgaacgaat agcacttttt gctgcatgtt
      661 ttccttttac tttaattaga tttgcaaggc ctgcagtagt tcctcctaat gctaaagcta
      721 ttgcagtatc taatagagca cccatttgat taactgtaat accttgccaa gctgctctaa
      781 atggagagta tgtaggtggg attgtataat cgccttgtaa ttgtcggtta attacttctt
      841 tgatccattg ttgtgagacg tctggatgaa aagattggat ttcgtttgca agtgtattga
      901 tttgttcttc tgttagagaa gtgacaggtt gaagttccat atttgtttca atttgtgata
      961 cttgttcaga agcgtataca gctgaaacac ttggaatcgc tgatacaatt aacacaattg
     1021 acgtcaaaaa aaccgaaata aatttcatta atttgttcat gagcttttct cctttttatt
     1081 tgtatctgct tacattttat catatactgt tattatagtc aaaaaaatat gctattatgt
     1141 taaaaaaata tttttcaaaa tataaatgga cggattta
//
LOCUS       DAYYQN010000120         1040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYYQN010000120 DAYYQN010000000
VERSION     DAYYQN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.138"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..>1040
                     /locus_tag="ACHBRQ_002021"
     CDS             <1..>1040
                     /locus_tag="ACHBRQ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9593611.1"
                     /translation="DAPSCPECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKC
                     YHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLND
                     FHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLKYD
                     RAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQLD
                     RKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEILNKLLSYSQDLKHHYQ
                     LYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAK
                     LEATNNLIKLIK"
BASE COUNT          330 a          224 c          167 g          319 t
ORIGIN      
        1 cgacgcccca tcttgccctg agtgcggaaa ccaattgaag aaatatgact ttcaaaaacc
       61 ttctaaaatt ccttatcttg aaacgactgg tatgcctact agaattctcc ttagaaagcg
      121 tcgattcaag tgctatcact gttcaaaaat gatggttgct gaaacttcta tcgtcaagaa
      181 gaatcaccaa atccctcgta tcatcaacca aaagattgct caaaagttaa ttgaaaagat
      241 ttctatgact gatattgccc atcagctttc catctcaact tcaactgtta ttcgtaagct
      301 caatgatttt cactttaaac atgatttttc ttgtcttcct gagattatgt cttgggatga
      361 gtatgctttt acaaagggaa agatgagctt cattgcgcaa gattttaaca atctcaatat
      421 catcactgtt cttgagggaa gaacacaagc tatcattcga aatcactttc ttaaatatga
      481 tagagccgtc cgatgtcgcg tcaaaattat tactatggat atgtttagtc cttactatga
      541 cttagctaga caacttttcc catgtgctaa aatcgtgttg gatcgctttc acattgtaca
      601 acatcttagc cgtgctatga gtcgtgtgcg tgttcaaatc atgaatcaat tggatcgaaa
      661 gtctcatgaa tacaaggcta tcaagcgcta ctggaagctc atacaacagg atagtcgtaa
      721 actcagcgat aaacattttt atcgccctac ttttcgtatg catttaacca ataaagagat
      781 tttaaacaag cttttgagct attcacaaga cttgaaacac cactatcagc tctatcaact
      841 cttgctgttt cactttcaga ataaggaacc ggagaaattt ttcggactca ttgaggacaa
      901 tctaaagcag gttcatcctc tttttcagac tgtctttaaa acctttctaa aggacaaaga
      961 gaaaatcgtc aacgcccttc aactacacta ttctaacgcc aaattggaag cgaccaataa
     1021 tctcatcaaa cttatcaagc
//
LOCUS       DAYYQN010000121          993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYYQN010000121 DAYYQN010000000
VERSION     DAYYQN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.141"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..993
                     /locus_tag="ACHBRQ_002022"
                     /pseudo
     CDS             1..993
                     /locus_tag="ACHBRQ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          293 a          213 c          179 g          308 t
ORIGIN      
        1 cttgtcctga gtgcggaagt caaatgaaga aatatgactt tcaaaaacct tctaaaattc
       61 cttatcttga aacgactggt atgcctacta gaattctcct tagaaagcgt cgtttcaagt
      121 gctatcattg ttctaaaatg atggtcgctg aaacttctat cgtcaagaaa aatcaccaaa
      181 ttcctcgtat tatcaaccaa aaaattgcgc aaaagttgat tgagaagatt tctatgaccg
      241 atattgctca tcagctggcc atttcaactt caactgtcat tcgcaagctc aatgattctc
      301 actttgagca tgatttttcg cgtcttcctg agattatgtc ctgggacgtt gaaacagtcc
      361 ggggagtgac tgtttcaatc gggagatgga gatgagcttt attgcgcaag attttaacaa
      421 tctcaatatc atcactgttc ttgaaggtag aacacaagct gtcatccgag atcactttct
      481 taaatatgat agagccgtcc gatgtcgcgt caaaatcatt acgatggata tgtttagccc
      541 ttactatgac ttggctaaac agcttcgctt tcaaatttct aggctcaggc tgaaacagtc
      601 tcccaggctg tttcactccc gaatgctaaa atcgttcttg atcgctttca tattgtacaa
      661 catcttagcc gtgctatgag tcgtgtgcgt gttcaaatca tgaatcagtt tcatcgaaaa
      721 tcccatgaat acaaggctat caagcgctac tggaagctca tacaacagga tagtcgtaaa
      781 ctcagcgata aacattttta tcgccctact tttcgtatgc atttaaccaa taaagagatt
      841 ttaaacaagc ttttgagtta ttcggaagac ttgaaacacc actatcaact ctatcagctc
      901 ttgctttttc acttccagaa taaggaacca gagaaatttt tcggactcat tgaggacaat
      961 cttaagcagg ttcatcctat ttttcagact gtc
//
LOCUS       DAYYQN010000122          932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYYQN010000122 DAYYQN010000000
VERSION     DAYYQN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.144"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            1..815
                     /locus_tag="ACHBRQ_002023"
                     /pseudo
     CDS             1..815
                     /locus_tag="ACHBRQ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238800.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          312 a          193 c          149 g          278 t
ORIGIN      
        1 agtataaaaa aattcaaact agaattcttc ttagaaaagg ccgttttaag tgctatcatt
       61 gctcaaaaat ggagctcgct gagacttcta tcgtcaagaa gaatcatcaa attccttgta
      121 tcatcaacca aaagattgct caaaagttaa ttgaaaagat ttctatgacc gacattgatc
      181 atcagttgtc tatttcaact tcaactgtca ttcgcaagat caataatttt cactttgagc
      241 atgatttttc gcgtcttcct gagattatgt cctaggacgt tgaaacagtc cggggagtga
      301 ctgtttcaat cgggagatgg agatgagctt tattgctcag gactttgata aactcaatat
      361 cataactgtt cttgagagca gaacacaagc catcatccga aatcccatga atacaaggct
      421 atcaagcgct actggaagct cattcaacaa gatagtcaga aactgagtga taaacgattt
      481 tatcgcccta cttttcgtat gcacttgact aataaagaaa ttctagataa acttttgagc
      541 tattccgaag acttgaaaca ccactataat ctctatcagc tcttgctttt ccacctctgt
      601 ttcagacttt gtttaaaacc ttcctcaaag ataaagagaa aatcgtcaac gcccttcaat
      661 taccttattc caacgccaaa ctggaagcca ccaataatct catcaaactt atcaagcaca
      721 atgcctttgg ttttaggaac tttgaaaact tcaaaaaaga aaggacgaaa tttgtccttt
      781 ctagatcttc gctttcttca acccactaca gttgacaaag aaccgaaaaa tgtctcgcct
      841 ctaatatagt caattgaaac aagaacaaga gaaaagagcc tcgtaaaagg tattgcaact
      901 tggtaatacc tttttgaggt tctttttgat at
//
LOCUS       DAYYQN010000123          878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYYQN010000123 DAYYQN010000000
VERSION     DAYYQN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.147"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(30..776)
                     /locus_tag="ACHBRQ_002024"
     CDS             complement(30..776)
                     /locus_tag="ACHBRQ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9593612.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNMATLENAGIMVKVVS"
BASE COUNT          266 a          187 c           92 g          333 t
ORIGIN      
        1 ttcctatctt gattcataca cttaacctct tacgaaacta ccttaaccat tatcccagca
       61 ttttctaatg tagccatatt ttgtttagaa agtttttcgt ctgttattac ttcatagact
      121 tgacttaaag caaatcttct tactgtaccc cttttatcaa atttacttga gtcagttagc
      181 acaatgactt tatccgacgc tgctgaaata tattgaacta cctcactgcg cattaaatct
      241 tttcctgtaa agcccatctc tttatcgtag ccatctgtcc caacaaaagc ttgacgcaca
      301 tgaaaagtct gtatcatttc ttttaataaa ggtcccacag taacctgtga atccttctga
      361 aactctccac caagaacaat aatacgacat gaatcataag ctctcacaaa atttgctata
      421 aaaaatgaat ttgttacaat cgtaacattt cttttttgct tgcaaatttc ctcagcaagt
      481 aaagcacagg tcgatccaga ttctatcatt attgtttcat tatctgacac caattttact
      541 gcttcctgaa caatttttct cttaatttca tagttaattg acaaacgtac atttaagtca
      601 tctccactat ttaatacagc atatccatgc tctctgtgta ataaaccttt tgactctaat
      661 ttatctaaat cttttctaat cgttactttc gatacattta atttttccga taatgtatta
      721 acgtcgatct tttcatattc tgatactaat ttaataattt gttccaatct tttcatttta
      781 cacctccgtt ttattctacc aaaataaaaa gcaaaaaaca acaaattaac ctttcgttcg
      841 taattgtttt tctttcgttt ttgtgatagg atagactt
//
LOCUS       DAYYQN010000124          690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYYQN010000124 DAYYQN010000000
VERSION     DAYYQN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.158"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            complement(180..>690)
                     /locus_tag="ACHBRQ_002025"
     CDS             complement(180..>690)
                     /locus_tag="ACHBRQ_002025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFM9593613.1"
                     /translation="CIGELTILQVLYGASLFSFLWMYGQRKQVVKVNMKTRMKCLGIG
                     LASLLIISLCFSLIHAQGSTNQTNLIGLQHQVPWFSFLLFLINASMVEEFLYREILWN
                     LVRKLDIRVALTSVLFALVHHPGTILAWCLYVSLGMFLGMVRYKSDLWGSMGLHLVWN
                     FLVYSLLLF"
BASE COUNT          265 a          125 c           86 g          214 t
ORIGIN      
        1 aatattttat tttggtttta ttatttcttt tcggaatttc tatataatat tttatttcta
       61 aaaaaattga aaaaatattt ctagtttctt tattttatat aggtaatata ttttatttct
      121 aaattaaaag agaatcccat aaaaactaca gatttatgag ataaatcagg tcacctattt
      181 taaaaaagca gcaaactata aactaaaaag ttccacacca aatgtaaccc catacttccc
      241 cataagtcag atttatagcg caccatacct aaaaacattc caagtgaaac gtacagacac
      301 caagctagaa tggttcctgg atgatgtact aaggcaaata aaacacttgt caaagcaact
      361 cgaatatcta attttctaac caagttccat aaaatttcac gatacagaaa ttcttcaacc
      421 atactcgcat tgattaagaa caataaaaat gaaaaccaag gaacttgatg ttgaaggcca
      481 attaaatttg tttgattcgt gcttccttga gcatgaatca ggctaaaaca tagacttata
      541 atcagtagac tagctagtcc aataccaagg catttcatcc tagttttcat attgaccttg
      601 accacttgtt ttcgttgacc atacatccat aaaaaagaaa aaagagacgc accatagaga
      661 acctgtagta tagttaactc accgatacaa
//
LOCUS       DAYYQN010000125          631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYYQN010000125 DAYYQN010000000
VERSION     DAYYQN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.163"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            105..584
                     /locus_tag="ACHBRQ_002026"
     CDS             105..584
                     /locus_tag="ACHBRQ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9593614.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          237 a           80 c          147 g          167 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggagcaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caagaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aaacaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 atgaaaaaaa ttagaaaaag tagttgacaa a
//
LOCUS       DAYYQN010000126          620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.denovo.165, whole genome shotgun sequence.
ACCESSION   DAYYQN010000126 DAYYQN010000000
VERSION     DAYYQN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.denovo.165"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            263..562
                     /locus_tag="ACHBRQ_002027"
     CDS             263..562
                     /locus_tag="ACHBRQ_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9593615.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          231 a          120 c          106 g          163 t
ORIGIN      
        1 agcatacgaa cctttagtaa aatcatttcc atgaaactag aatagagccc tcttagcaaa
       61 aatcattatt ttaatttatt tctaatcact ccttgacata aataactctc accaataaaa
      121 gactatgtct taaaaaaatg gtataataaa atcaatactt gggcttgatg gctatgctac
      181 taataacaat taggagagaa aatcaggcac ttgttaacaa caaggattat ccccttgaga
      241 tgaaaggaac tttagaaatc ttatgatgaa catgcaaaac atgatgcgtc aagcacaaaa
      301 acttcaaaaa caaatggaac aaagccaagc tgaacttgct gctatgcaat ttgttggcaa
      361 atctgctcaa gatcttgtcc aagcgacctt aactggcgat aagaaagttg tcagcattga
      421 tttcaatcca gctgtcgttg acccagagga ccttgagact ctttctgata tgaccgttca
      481 agccatcaac tctgctcttg aacaaatcga tgaaactacc aagaaaaaac tgggtgcttt
      541 cgctgggaaa ttacctttct aaaaacaagg agctagaaca atgcttgtcg ataacaaagg
      601 ctaagaaagg tgcaaaaatg
//
LOCUS       DAYYQN010000127        12909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.guided.051, whole genome shotgun sequence.
ACCESSION   DAYYQN010000127 DAYYQN010000000
VERSION     DAYYQN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12909
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.guided.051"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            <1..684
                     /gene="erm"
                     /locus_tag="ACHBRQ_002028"
     CDS             <1..684
                     /gene="erm"
                     /locus_tag="ACHBRQ_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFM9593616.1"
                     /translation="NQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSH
                     LFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRAS
                     DIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRH
                     TTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTITYEQVLSIFN
                     SYLLFNGRK"
     gene            complement(941..2188)
                     /locus_tag="ACHBRQ_002029"
     CDS             complement(941..2188)
                     /locus_tag="ACHBRQ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9593617.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            2368..2589
                     /locus_tag="ACHBRQ_002030"
     CDS             2368..2589
                     /locus_tag="ACHBRQ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9593618.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            2706..3203
                     /locus_tag="ACHBRQ_002031"
     CDS             2706..3203
                     /locus_tag="ACHBRQ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9593619.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3292..3684
                     /locus_tag="ACHBRQ_002032"
     CDS             3292..3684
                     /locus_tag="ACHBRQ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9593620.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            3668..6115
                     /locus_tag="ACHBRQ_002033"
     CDS             3668..6115
                     /locus_tag="ACHBRQ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9593621.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6118..8295
                     /locus_tag="ACHBRQ_002034"
     CDS             6118..8295
                     /locus_tag="ACHBRQ_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9593622.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8292..9293
                     /locus_tag="ACHBRQ_002035"
     CDS             8292..9293
                     /locus_tag="ACHBRQ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9593623.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9290..10225
                     /locus_tag="ACHBRQ_002036"
     CDS             9290..10225
                     /locus_tag="ACHBRQ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9593624.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10539..10586
                     /locus_tag="ACHBRQ_002037"
                     /pseudo
     CDS             10539..10586
                     /locus_tag="ACHBRQ_002037"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10602..12521
                     /gene="tet(M)"
                     /locus_tag="ACHBRQ_002038"
     CDS             10602..12521
                     /gene="tet(M)"
                     /locus_tag="ACHBRQ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM9593625.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            12640..12807
                     /locus_tag="ACHBRQ_002039"
     CDS             12640..12807
                     /locus_tag="ACHBRQ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9593626.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT         4143 a         2314 c         2658 g         3794 t
ORIGIN      
        1 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
       61 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
      121 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact
      181 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
      241 gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccgt
      301 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
      361 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca
      421 gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat
      481 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc
      541 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc
      601 aaagtaaaca atttaagtac cattacttat gagcaagtat tgtctatttt taatagttat
      661 ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg gaaagttaca
      721 cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt ccaagaagct
      781 aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa ttcccactaa
      841 gcgctcggga ccccttgtag gaaaatgtcc taagtgggat atctgtcaac tggtatggtt
      901 gactaaaaat acttcctacg aaaatgtagg gggtattttt ttacgaaaaa atacaatcga
      961 ttcttaaaaa gaaaaatttt tgattggcaa aaccataaca agttcgtttt agggttttga
     1021 ttttgcgatt gatgccttct aaaggaccat tagagtattc aaatttagcg ctatttaaga
     1081 catattttct gttttgacga agggtttgaa tagcagtatc catttctgta ttggtttttt
     1141 ggtagtctaa gatggttgac tctagtaatt cactattgcg ctcgtttagg gctttcgtga
     1201 tatcttggta agtttggtat acttcagcga acttggaaaa tttactagta atgagatcaa
     1261 cagcattttg gcgagtcata tattgtttaa cgccgcgaag aaaaactact tcttcagggt
     1321 gaagatcttc agctttttta tggaatagct tccaatgtga cttcataatt ttatattctt
     1381 ggctctgttt atcaagttgc tttaggatag agatacgaca attgtccaaa gcgcgaccag
     1441 ctaattgtac aaggtggaag cgatcaataa tgatattggc attagggaaa aggcgataga
     1501 taaaactttg atattgagca tttaaatcaa ttacaactga ttgaacgcat tcgcgttcgg
     1561 cttttgaata acgactttca aaataatcaa caatggtagg tgatagacga tcctgtaact
     1621 ttgtgacaat ttggtgggtt tcagcgtcac aacagataaa ggacatcaca gacttaattg
     1681 aacgaaactc gtcaaaacat agatgcttag gcaacttagc cacacgatag tgtggttcca
     1741 tgcgctctaa gattgttcga cgaacactgc taggagagca gtgacacatt tcagcaataa
     1801 gctgaccaga taagccttta cgagctaaaa gcatgatttg atttttgaga tcactggata
     1861 aggtttgatt ttctttggtt aaattagtaa tagcaccaaa agtagtatgg catgatttac
     1921 atttatagcg ttgtttacga agctctagtt catatcttct cccatttaaa cttgccagtc
     1981 gtacatgagt tttgcgaaag ccatccttat taactgtggg aaagccacag ttacgacaac
     2041 gattaatcgg ataagaaaga gtagctgtta ttagcgttat atactcttta acagaatcgt
     2101 tgttgtgttc agcttcttca acagaaataa ttttaatatt tttatcttta attccaagaa
     2161 tatttaggat agaatcatta tgggacattt gtttaacctt ctttcatgat ttttgtggtg
     2221 aattgattgt ataacgaggg gacagcaaat gtcctctttt ttgtataaaa aaatctggca
     2281 tggaatctct atccatacca gaaagtgtat accccaaaaa aataactcaa atacaaattc
     2341 attgaatata gagaggagaa catttttatg aattttggac aaaaccttta taactggttt
     2401 ctatcaaacg ctcaatcact ggtgctttta gcaatcgttg tgattggctt gtatcttggc
     2461 ttcaagcgtg agtttagcaa actgattggc tttttaatta ttgcgattat tgcggttggc
     2521 ttagtcttca acgctgctgg agtaaaagac attttactag agctattcaa tcgcattatt
     2581 ggtgcttaaa taaaaccgtt cttttgtgga atataagtgg ttttcttatg ttccgcaaag
     2641 gaatggtaca ccaaacgaag tgcggtaggg atttttgaat ctctacaaag aaaggacgtg
     2701 aatatatgga cgatatgcaa gtctatattg cgaatttagg caaatacaat gagggcgaat
     2761 tggtcggtgc gtggtttacc tttcccattg actttgagga agtcaaagag aaaatcggct
     2821 tgaatgatga atatgaggaa tacgccattc atgactacga gttacccttt acggttgacg
     2881 aatacacttc cattggcgaa ctcaatcgac tatgggaaat ggtatcggaa ttacccgaag
     2941 aattacaatc ggagctatct gctctgctca ctcatttttc aagcattgaa gaactaagcg
     3001 aacatcaaga ggatattatc attcattccg attgtgatga tatgtatgac gtggcacgct
     3061 actacattga agaaacgggt gctttaggcg aagtaccagc tagtcttcaa aactatattg
     3121 attatcaagc ctatggtcgg gatttagacc tttcaggaac gtttatctca accaatcatg
     3181 ggatttttga aatcgtctat taaatctgtc ggtacattac tactggcaga ttttctattt
     3241 tacggggtgg ctcaatcagc tacccctatt ttttatgaaa ggattgatta catgaagaaa
     3301 atacgaagct ataccagtat ctggtctgtg gaaaaggtac tgtattctat caatgatttt
     3361 agacttccgt ttcccataac ctttacgcaa atgacatggt ttgtcgtgtc actctttgca
     3421 gtgatgatac ttggcaactt gccccctctt tccatgatag agggagcatt tctcaaatac
     3481 tttgggattc ctgtggcttt cacatggttt atgtctacaa aaacttttga tggtaaaaag
     3541 ccttatggat ttttgaagtc tgtcattgct tatgcactgc gaccaaagct gacctatgca
     3601 ggaaaaaaag taacgcttgg cagaaaccag ccacaagaag ccattacagc agttaggagt
     3661 gaattttatg gcatatccaa ttaaatacat tgaaaacaat ctcgtctgga ataaagacgg
     3721 ggaatgttat gcttactatg agcttgttcc ttacaattac tcatttctaa gtccagaaca
     3781 gaaaatacaa gtgcatgatt ctttcagaca gcttatcgca caaaatcgtg atggcaaaat
     3841 tcatgcttta caaatcagta cagaatccag catacgttct gcacaagagc gttccaaaaa
     3901 tgaagtcact ggcaagctca aagcggttgc ctatgacaaa atcgaccaac agacagacgc
     3961 tttaatatcc atgattggcg aaaatcaagt gaactaccgt ttctttatcg gctttaagtt
     4021 gcttctcaac gatcaggagt tttctatgaa aagtcttacc gttgaagcaa aaaatgcttt
     4081 gtctgatttt gtctatgatg tgaaccataa gctgatgggc gattttgtta gtatgagtaa
     4141 tgatgaaatc ctgcgttttc agaagatgga aaagctctta gaaaataaaa tctctcgtcg
     4201 tttcaaaatc cgcaggttag ataaggacga cttcggctat ctgattgaac acctttacgg
     4261 acagacaggc actgcctatg aagagtatga gtaccatcta tcaaagaaaa agctggataa
     4321 tgaaacgctg attaaatact atgacttgat taagcctact cgctgtttgg tggaagaaaa
     4381 acagcgatat ttgaaaatcc agcaggaaga tgaaaccgtc tatgtagctt actttaccat
     4441 taacagcatt gtcggagaac tggacttccc gtcctctgaa atcttctact accagcaaca
     4501 gcaatttaca ttcccgattg atacgtcaat gaatgtggaa attgtagcga atcgtaaagc
     4561 cctatctact gtccgcaata aaaagaaaga actgaaagac ttggataacc acgcttggca
     4621 aagtgataat gaaaccagct ccaatgtggc ggaagctctg gaaagtgtga atgagctgga
     4681 aaccaattta gaccaaagca aggaatctat gtacaagctg tcttatgtgg taagggtatc
     4741 agcaaatgat cttgacgaac tcaaacgtcg ttgtaatgaa gtgaaagatt tttatgacga
     4801 tttaagcgta aaactggtac gaccatttgg ggatatgctc ggcttacatg aagaattttt
     4861 acctgccagc aagcgttata tgaatgatta tattcaatac gtgacctctg atttcctcgc
     4921 tggtttaggt tttggtgcta ctcaaatgct gggggaaaat gaggggattt atgttggcta
     4981 cagcttagat actggacgca atgtctatct gaaacctgct cttgccagtc aaggggttaa
     5041 gggttcagta accaatgcgt tagcgtcggc ttttgttggt tcgctgggtg gtggtaaatc
     5101 ctttgcgaat aaccttatcg tctattatgc ggtgctttat ggggcacaag cagtgattgt
     5161 agacccaaaa gcagaacgtg gcagatggaa agaaaccttg ccagagattt cccatgaaat
     5221 caatatcgtc actctgactt ctgatgagaa aaacaaaggc ttacttgacc cttatgtgat
     5281 tatgaaaaat cccaaagatt ctgaatcact ggctattgat attctgacat tccttacggg
     5341 gatttcctct cgtgatgggg aacgcttccc aatccttaga aaagccattc gtgcagtaac
     5401 caatagtgaa gtacgagggt tgatgaaagt gattgaggaa ttacgggttg agaatacgcc
     5461 actaagtacc agtatagccg accatatcga aagttttaca gactatgact ttgcacattt
     5521 attattcagt aatggttatg tggagcagtc tatcagctta gaaaaacaac tgaacattat
     5581 acaggttgcg gacttggtac ttcccgacaa ggaaacttcc tttgaggaat ataccactat
     5641 ggagctttta tccgttgcta tgctgattgt cattagtacc tttgctttag actttatcca
     5701 tacagaccga agcattttca agattgtaga tttagacgaa gcatggagct ttttacaggt
     5761 agcacaagga aaaacactat ctatgaagct ggttcgggct ggtcgtgcta tgaacgctgg
     5821 ggtatatttc gtgacccaaa atacagacga cctcttagat gaaaaactga aaaataacct
     5881 cggcttaaaa tttgcatttc gttccactga ccttaacgag attaaaaaga ccttagcctt
     5941 ttttggtgta gacccagagg acgaaaacaa tcagaagcga ttgcgtgatt tggaaaacgg
     6001 gcaatgcctt atcagtgatt tatatggtcg tgtcggtgtg atacagttcc accctgtatt
     6061 tgaagaactg ctccatgcct ttgataccag accacctgtg cgaaaagagg tgtaaatgtg
     6121 aaaccatcaa tagtaaacag aataaaatca aactggacgc tgaaacgtct aggtaaagtg
     6181 gcaatgacag tggctttcac acttgtgatt gccatttttc ttttagccat gctgggaacg
     6241 gtggttcaag ctgcgggctt ggtagatgat acggtcaatg tggcaaatga atacagccga
     6301 tacccacttg aaaactatca actggatttt tatgtggata atagctgggg ctggcttccg
     6361 tggaactggt cggacgggat tggaaaacag gtcatgtatg gactatatgc cattaccaat
     6421 tttatttgga caatcagttt gtatgtttcc aatgcgacag gttacttagt acaggaagcc
     6481 tattccttag acttcatttc cgctacagca gattccattg gtaagaatat gcagacctta
     6541 gctggtgtga gtgcaaacgg attttcaaca gagggtttct atgttggatt cctcttactc
     6601 ttgattttgg ttcttggggt ttatgttgcc tatacgggac tgataaagag agaaaccaca
     6661 aaggcaattc atgccattat gaattttgtg ctggtgttta tcctatcggc ttcctttatt
     6721 gcctacgctc ccgactacat taaaaaaatc aatgactttt catcagacat cagtaatgcc
     6781 agtttatcac ttggcacgaa gattgtcatg ccccattccg atagtcaagg caaggacagc
     6841 gtggacttaa tcagagatag cctgttttcc atacaggttc agcaaccgtg gctactgctt
     6901 caatacaaca gttcagacat tgaaagtatc ggtattgacc gtgtggaaag cctgctctcc
     6961 accagcccag attccaacaa tggcgaagac agagaaaaaa ttgttgcgga agaaattgaa
     7021 gacagaagca ataccaatct aaccattaca aagaccatta accgtttagg tacagtcttc
     7081 ttcctatttg tcttcaatat tgggatttcc atatttgtat tcctattaac aggaatcatg
     7141 attttctcgc aggtactttt tatcatctat gctatgtttc tgcctgtgag ctttatttta
     7201 agcatgattc catcatttga tggtatgtca aaacgagcca taacaaagct ctttaatacc
     7261 attttgacac gagctggaat cacattgatt attacgacag catttagtat ttcaaccatg
     7321 ctctatacct tatcggctgg ttatccgttc tttttgattg cttttctaca gattgtgacc
     7381 tttgcaggaa tctacttcaa gctgggcgat ttaatgagta tgttttctct acagagtaac
     7441 gattctcaaa gtgtgggaag tcgtgtgatg agaaaacctc gtatgcttat gcacgctcac
     7501 atgcaccgtc tacagcggaa acttggacgt tccatgacta ctctaggggc tgggtctgcc
     7561 attgttacag gtaaaaaagg acagtcgggt tcggggagtt ctgcaaggac acaagcagat
     7621 cactcccgac cagacggaaa ggaaaaatca acacttggaa aacgtatcgg tcaaaccatc
     7681 ggtacagtag ctgataccaa agacagaatg gtagacactg ctagtggttt gaaagaacag
     7741 gttaaagatt tgccgaccaa tgcaagatat gcagtatatc aaggaaaatc caaagtaaaa
     7801 gagaatgtcc gtgatttaac cagtagtatt tctcaaacca aagcggacag agccagtgga
     7861 cgcaaggaac agcaggaaca aaggcgaaaa accattgcga agcgtcgctc tgaaatggaa
     7921 caggtcaaac agaaaaaaca gcctgcttct tctgttcatg aaagaccgac tacaagacaa
     7981 gaacaatatc atgatgaaca gacctcaaaa cagtctaata ttcagacttc atataaggaa
     8041 tctcaacaag ccaaacaaga gcgtccagca gttaagtccg atttttcaag tccaaaagtg
     8101 gaacgccaag gcaataccgt tcaagaaaaa accgttcaaa agccagcaac ttcaaccact
     8161 acagcagata gaacttcaca acgtccaatc acaaaagaac gtccgtctac tgttcaaaga
     8221 gtaccactac aaaatacaag aagtagacca ccaatcaaaa ccgccaccat taagaaagtc
     8281 ggtaagaaac catgaagttg aaaactttag tgattggtgg ttctggatta ttcttgatgg
     8341 tcttctcact gcttctgttt gttgccattt tattttcaga tgaacaggac agcggaattt
     8401 ccaatattca ttatggaggt gtgaatgttt ccgcagaagt gctggctcat aagcctatgg
     8461 tagaaaaata tgccaaagaa tatggcgttg aagaatatgt caacatactt cttgcgatta
     8521 tacaggtgga atcgggcggt actgcggaag atgttatgca gtcctcggaa tccctcggtc
     8581 ttccacctaa ttcattgagt acagaagaat ccattaagca aggtgtgaag tatttcagtg
     8641 aattattagc cagtagcgaa aggctcagtg tagatttaga atcggttatc cagtcctaca
     8701 attatggtgg tggtttctta gggtatgtgg ctaatcgtgg aaataaatat acctttgaac
     8761 tggctcaaag tttctcaaaa gagtattcag gtggcgaaaa agtgtcttac cccaatccca
     8821 tagccatacc tatcaatggg ggctggcgat acaactatgg caatatgttt tatgtgcaac
     8881 tggtaacgca gtatcttgtc acaacagagt ttgatgatga tacggtacaa gccatcatgg
     8941 acgaagcact gaaatatgag ggctggcgat acgtttacgg tggagcttcc ccgactactt
     9001 cttttgattg tagcggactg acacaatgga cgtatggaaa agctggaatt aacttaccac
     9061 gaaccgcaca acagcaatat gatgtgaccc agcatatccc actatcggaa gcacaagctg
     9121 gcgatttggt tttctttcat tctacctata acgctggctc ttatattact catgttggga
     9181 tataccttgg caataaccgt atgtttcatg caggcgaccc aatcggttat gccgacttaa
     9241 caagccccta ctggcaacag catttagtgg gagcaggacg aatcaaacaa tgagaaagga
     9301 agatttaatg atgaaattta gaaaaaatca gaataaagaa aaacagatac caaaggaaaa
     9361 gaaacctcgt gtctactata aggtcaatcc tcataaaaag gttgtgattg ccttgtgggt
     9421 acttttaggg cttagtttca gctttgcgat attcaagcac tttacagcta tagatactca
     9481 tactattcac gaaacaacta tcatagaaaa ggaatacgtt gatactcatc atgtagaaaa
     9541 ttttgtagag aactttgcga aagtctacta ttcatgggag caatccgata agtccattga
     9601 taatcgaatg gaaagtctaa aaggctatct gacagatgaa cttcaagctc tcaatgttga
     9661 tacagtacgc aaagatattc ctgtatcgtc ttctgtaaga ggatttcaga tatggacggt
     9721 agagccaact ggcgacaatg agtttaatgt aacctacagt gtagaccagc tcattacaga
     9781 gggagaaaat acaaagaccg tccactctgc ttatatagtg agtgtctatg tagatggttc
     9841 tggaaatatg gtactggtta agaatccgac cattaccaac atacctaaga aatcaagtta
     9901 taaaccaaaa gccattgaaa gtgaggggac ggttgattcc attacaacca atgaaatcaa
     9961 tgagttttta acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta
    10021 ctatgtgaat gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt
    10081 aaatcctatt cacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat
    10141 cgaccagcag accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg
    10201 gagtaattgg aagattatag aataacaaat attggtacat tattacagct attttgtaat
    10261 cacgtactct ctttgataaa aaattggaga ttcctttaca aatatgctct tatgtgctat
    10321 tatttaagta tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
    10381 tgtcaatttg atagtggaaa caaataattg gatgtccttt tttaggaggg cttagttttt
    10441 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta
    10501 tgctttgtat gcctatggtt atgcataaaa atcccagtga taagagtatt tatcactggg
    10561 atttttatgc ccttttgggc ttttgaatgg aggaaaatca catgaaaatt attaatattg
    10621 gagttttagc tcatgttgat gcgggaaaaa ctaccttaac agaaagctta ttatataaca
    10681 gtggagcgat tacagaatta ggaagcgtgg acagaggtac aacgaaaacg gataatacgc
    10741 ttttagaacg tcagagagga attacaattc agacggcgat aacctctttt cagtggaaaa
    10801 atactaagat aaacatcata gacacgccag gacatatgga ttttttagca gaagtatatc
    10861 gttcattatc agtattagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag
    10921 cacaaactcg tatattgttt catgcactta ggaaaatagg tattcccaca atctttttta
    10981 tcaataagat tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga
    11041 aactttctgc ggaaattgta atcaaacaga aggtagaact gcatcctaat atgcgtgtaa
    11101 tgaactttac cgaatctgaa caatgggata tggtaataga aggaaatgat taccttttgg
    11161 agaaatatac gtctgggaaa ttattggaag cattagaact cgaacaagag gaaagcataa
    11221 gatttcataa ttgttccctg ttccctgttt atcacggaag tgcaaaaaac aatataggga
    11281 ttgataacct tatagaagtg attacgaata aattttattc atcaacacat cgaggtcagt
    11341 ctgaactttg cggaaaagtt ttcaaaattg agtattcgga aaaaagacag cgtcttgcat
    11401 atatacgtct ttatagtggc gtactgcatt tgcgagattc ggttagaata tcggaaaagg
    11461 aaaaaataaa aattacagaa atgtatactt caataaatgg tgaattatgt aaaatcgata
    11521 aggcttattc cggggaaatt gttattttgc agaatgagtt tttgaagtta aatagtgttc
    11581 ttggagatac aaagctattg ccacagagag agagaattga aaatcccctc cctctgctgc
    11641 aaacgactgt tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag
    11701 aaatctccga cagtgacccg cttctgcgat attatgtgga ttctgcgaca catgaaatca
    11761 tactttcttt cttagggaaa gtacaaatgg aagtgacttg tgctctgctg caagaaaagt
    11821 atcatgtgga gatagaaata aaagagccta cagtcattta tatggaaaga ccgttaaaaa
    11881 aagcagagta taccattcac atcgaagttc caccgaatcc tttctgggct tccattggtc
    11941 tatctgtagc accgcttcca ttagggagcg gagtacagta tgagagctcg gtttctcttg
    12001 gatacttaaa tcaatcgttt caaaatgcag ttatggaggg gatacgctat ggctgtgaac
    12061 aaggattgta tggttggaat gtgacggact gtaaaatctg ttttaagtat ggcttatact
    12121 atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag
    12181 tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc
    12241 cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag
    12301 acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc
    12361 aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt
    12421 taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc
    12481 ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata
    12541 taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt
    12601 gtaaaggagg agatagttat tataaactac aagtggatat tgtgtcctgt atgtggaaat
    12661 aaaacacgat taaagataag ggaagatact gaattaaaaa aattccccct ctattgtccg
    12721 aaatgcagac aagaaaattt aattgaaata aagcagttca aagtaactgt gattacagag
    12781 ccagacgcaa agacgcagag ccgataaaat gagattaata caatctcatt ttatcggctc
    12841 tttccgttat gtatggattc ttttaattag tcttcgatgt ttcttgcttc gttgataccg
    12901 ctggctaaa
//
LOCUS       DAYYQN010000128         2110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 24 isolate GC127473
            SAMN42747947-rid22605673.guided.115, whole genome shotgun sequence.
ACCESSION   DAYYQN010000128 DAYYQN010000000
VERSION     DAYYQN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN42747947
            Sequence Read Archive: SRR29924709
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002291582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002291582.1
            Genome Coverage       :: 434x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 18:41:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,858
            CDSs (with protein)               :: 1,858
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 71 of 133
            Pseudo Genes (incomplete)         :: 70 of 133
            Pseudo Genes (internal stop)      :: 44 of 133
            Pseudo Genes (multiple problems)  :: 46 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN42747947-rid22605673.guided.115"
                     /strain="24"
                     /isolate="GC127473"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2022"
                     /collected_by="UZ Leuven"
     gene            251..1045
                     /locus_tag="ACHBRQ_002040"
                     /note="aph(3')-IIIa"
     CDS             251..1045
                     /locus_tag="ACHBRQ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9593627.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1529..2022
                     /locus_tag="ACHBRQ_002041"
                     /pseudo
     CDS             1529..2022
                     /locus_tag="ACHBRQ_002041"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          705 a          332 c          489 g          584 t
ORIGIN      
        1 taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa attttgtata
       61 ataggagtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
      121 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
      181 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
      241 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
      301 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
      361 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
      421 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
      481 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
      541 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
      601 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
      661 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
      721 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
      781 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
      841 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
      901 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
      961 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     1021 tattttactg gatgaattgt tttagtacct agatttagat gtctaaaaag ctttaactac
     1081 aagcttttta gacatctaat cttttctgaa gtacatccgc aactgtccat actctgatgt
     1141 tttatatctt ttctaaaagt tcgctagata gagttctata ttagagatgt aaagagtttt
     1201 ggtgaagagg tttgtgagta tggcttttat aaccagggtg caatgagaag cactaacagc
     1261 agctagctta agaacgcata ggatatactt tttatggaag tcacagagga ggaataaaat
     1321 ttgaacagga ttaacagagt aacctctatt cttattcagc tgcaatcaaa aaaaaataat
     1381 tcctgccaaa gaaattgcac agcgatttaa tataagctta aggacagttt acagagatat
     1441 ccggacactt gaagaagccg gaataccaat tggatctgag gccggaaaag gatattttct
     1501 tgtagagggc ttcctacttc cgccggtaat gtttacagcg gcggaagtgg gtgcattgat
     1561 tacagcaggg aaatttttaa attgccatgg agatgaatca tttataaagg attttgattc
     1621 agctatgtat aaaatcaaat ctattttgaa gcatggtgaa aaaaactatg cacaggagtt
     1681 ggagaacagt attaacgtgt acagcacatc tggacagaaa aatacattgg cggataacgt
     1741 tattgcagca attcagactg caatctgcaa taaaagggta atatcaattc aatatcctgc
     1801 atcaggaggg caagaaccgg aaagcagaat gatagagcct atatcactgg ggttttatga
     1861 acagaactgg tacttaatcg gttttgcagg ctaagaaatg aatataggaa ttttcgtgtg
     1921 gatcggatta aaagcatttg catagaagaa gagctctgtc aaagccatga tggctctttg
     1981 gaacaaatat taaaacaaat gctttcatat aaaaaactat atgccctaca atgccgttgt
     2041 aaaaattggg gaaaagggat atattgattt tatatctttc tcaaaataaa tataaaagcc
     2101 ctttaaagag
//
